title: Nesterenkonia sp. strain F, a halophilic bacterium producing acetone, butanol, and ethanol under aerobic conditions.
abstract: The moderately halophilic bacterium Nesterenkonia sp. strain F, which was isolated from Aran-Bidgol Lake (Iran), has the ability to produce acetone, butanol, and ethanol (ABE) as well as acetic and butyric acids under aerobic and anaerobic conditions. This result is the first report of ABE production with a wild microorganism from a family other than Clostridia and also the first halophilic species shown to produce butanol under aerobic cultivation. The cultivation of Nesterenkonia sp. strain F under anaerobic conditions with 50 g/l of glucose for 72 h resulted in the production of 105 mg/l of butanol, 122 mg/l of acetone, 0.2 g/l of acetic acid, and 2.5 g/l of butyric acid. Furthermore, the strain was cultivated on media with different glucose concentrations (20, 50, and 80 g/l) under aerobic and anaerobic conditions. Through fermentation with a 50 g/l initial glucose concentration under aerobic conditions, 66 mg/l of butanol, 125 mg/l of acetone, 291 mg/l of ethanol, 5.9 g/l of acetic acid, and 1.2 g/l of butyric acid were produced. The enzymes pertaining to the fermentation pathway in the strain were compared with the enzymes of Clostridium spp., and the metabolic pathway of fermentation used by Nesterenkonia sp. strain F was investigated.
authors.affiliation: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 81746-73441, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Amiri
authors.affiliation: Molecular Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Azarbaijani
authors.affiliation: Molecular Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Parsa Yeganeh
authors.affiliation: Molecular Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran.|Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, ACECR, Tehran, Iran.
authors.forename: Abolhassan
authors.initials: A
authors.lastname: Shahzadeh Fazeli
authors.affiliation: Molecular Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran.|Agricultural Biotechnology Research Institute of Iran (ABRII), AREEO, Karaj, Iran.|Biofuel Research Team (BRTeam), Karaj, Iran.
authors.forename: Meisam
authors.initials: M
authors.lastname: Tabatabaei
authors.affiliation: Molecular Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran.|Agricultural Biotechnology Research Institute of Iran (ABRII), AREEO, Karaj, Iran.
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
authors.affiliation: Industrial Biotechnology Group, Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.|Department of Chemical Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Keikhosro
authors.initials: K
authors.lastname: Karimi
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000096:Acetone / Q000378:metabolism*; D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways; D000440:Butanols / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism*; D005798:Genes, Bacterial; D060106:Lakes / Q000382:microbiology; D008835:Micrococcaceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D000440:Butanols; D002087:Butyrates; D000096:Acetone; D000431:Ethanol; D019342:Acetic Acid