title: Secretome profile analysis of hypervirulent Mycobacterium tuberculosis CPT31 reveals increased production of EsxB and proteins involved in adaptation to intracellular lifestyle.
abstract: Epidemiological information and animal models have shown various Mycobacterium tuberculosis phenotypes ranging from hyper- to hypovirulent forms. Recent genomic and proteomic studies suggest that the outcome of infection depends on the M. tuberculosis fitness, which is a direct consequence of its phenotype. However, little is known about the molecular and cellular mechanisms used by mycobacteria to survive, replicate and persist during infection. The aim of this study was to perform a comprehensive proteomic analysis of culture filtrate from hypo- (CPT23) and hypervirulent (CPT31) M. tuberculosis isolates. Using two-dimensional electrophoresis we observed that 70 proteins were unique, or more abundant in culture filtrate of CPT31, and 15 of these were identified by mass spectrometry. Our analysis of protein expression showed that most of the proteins identified are involved in lipid metabolism (FadA3, FbpB and EchA3), detoxification and adaptation (GroEL2, SodB and HspX) and cell wall processes (LprA, Tig and EsxB). These results suggest that overrepresented proteins in M. tuberculosis CPT31 secretome could facilitate mycobacterial infection and persistence.
authors.affiliation: Genomic Sciences Program, Autonomous University of Mexico City, 03100, Mexico.
authors.forename: Fernado
authors.initials: F
authors.lastname: Vargas-Romero
authors.affiliation: Medical Proteomics Unit, National Institute of Genomic Medicine (INMEGEN), 14610, Mexico.
authors.forename: Nora
authors.initials: N
authors.lastname: Guitierrez-Najera
authors.affiliation: Medical School, Universidad Nacional Autónoma de México, 04510, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Mendoza-Hernández
authors.affiliation: Genomic Sciences Program, Autonomous University of Mexico City, 03100, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ortega-Bernal
authors.affiliation: Department of Experimental Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán (INCMNSZ), 14080, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.affiliation: Genomic Sciences Program, Autonomous University of Mexico City, 03100, Mexico castanon.mauricio@gmail.com.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Castañón-Arreola
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D005838:Genotype; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D014376:Tuberculosis / Q000382:microbiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Mycobacterium tuberculosis; hypervirulent; lifestyle; secretome profile