title: Beneficial effect of medicinal plants on the contractility of post-hypoxic isolated guinea pig atria - Potential implications for the treatment of ischemic-reperfusion injury.
abstract: Context Ischemic-reperfusion injury is accompanied by a decreased contractility of the myocardium. Positive-inotropic agents have proven useful for treating this condition but may exert serious side-effects. Objective In this study, aqueous preparations from Abelmoschus esculentus L. Moench (Malvaceae), Annona muricata L. (Annonaceae), Bixa orellana L. (Bixaceae), Cecropia peltata L. (Moraceae), Erythrina fusca Lour. (Fabaceae), Psidium guajava L. (Myrtaceae) and Terminalia catappa L. (Combretaceae) were evaluated for their ability to improve the decreased contractility of isolated guinea pig atria after hypoxic stress. Materials and methods Guinea pig atria isolated in Ringer-Locke buffer gassed with 100% O2 at 30 °C were exposed for 5 min to hypoxia, then allowed to recover in oxygenated buffer alone or containing a single plant extract (0.001-1 mg/mL). The contractility (g/s) and beating frequency (beats/min), as well as troponin C contents of the bathing solution (ng/mL), were determined and expressed as means ± SDs. Results The extracts of A. muricata, B. orellana, C. peltata and T. catappa caused an increase in the contractility compared to untreated atria of 340 ± 102%, 151 ± 13%, 141 ± 14% and 238 ± 44%, respectively. However, the latter two preparations increased the troponin C contents of the bathing solution to 36 ± 11 and 69 ± 33, compared to the value of 11 ± 3 ng/mL found with untreated atria. Conclusions Preparations from A. muricata and B. orellana may possess positive-inotropic properties which may improve the contractility of the post-hypoxic myocardium. Studies to assess their usefulness in ischemic-reperfusion injury are warranted.
authors.affiliation: a Department of Physiology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Robbert
authors.initials: R
authors.lastname: Bipat
authors.affiliation: a Department of Physiology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Jerry R
authors.initials: JR
authors.lastname: Toelsie
authors.affiliation: a Department of Physiology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;|b Department of Pharmacology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Indira
authors.initials: I
authors.lastname: Magali
authors.affiliation: a Department of Physiology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;|b Department of Pharmacology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Rubaina
authors.initials: R
authors.lastname: Soekhoe
authors.affiliation: c Department of Pharmacy , University of Utrecht , Utrecht , The Netherlands ;
authors.forename: Karin
authors.initials: K
authors.lastname: Stender
authors.affiliation: d Department of Clinical Chemistry , University of Groningen , Groningen , The Netherlands.
authors.forename: Angelique
authors.initials: A
authors.lastname: Wangsawirana
authors.affiliation: a Department of Physiology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;|b Department of Pharmacology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Krishan
authors.initials: K
authors.lastname: Oedairadjsingh
authors.affiliation: a Department of Physiology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;|b Department of Pharmacology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Jennifer
authors.initials: J
authors.lastname: Pawirodihardjo
authors.affiliation: b Department of Pharmacology, Faculty of Medical Sciences , Anton de Kom University of Suriname , Paramaribo , Suriname ;
authors.forename: Dennis R A
authors.initials: DR
authors.lastname: Mans
mesh_terms: D000818:Animals; D016275:Atrial Function / Q000187:drug effects*; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006325:Heart Atria / Q000187:drug effects* / Q000503:physiopathology; D000860:Hypoxia / Q000150:complications*; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D013997:Time Factors
chemical_list: D002316:Cardiotonic Agents; D010936:Plant Extracts
keywords: Hypoxia; positive-inotropism; troponin C