title: Characterization of the general stress response in Bartonella henselae.
abstract: Bacteria utilize a general stress response system to combat stresses from their surrounding environments. In alpha-proteobacteria, the general stress response uses an alternate sigma factor as the main regulator and incorporates it with a two-component system into a unique regulatory circuit. This system has been described in several alpha-proteobacterial species, including the pathogens Bartonella quintana and Brucella abortus. Most of the studies have focused on characterizing the PhyR anti-anti-sigma factor, the NepR anti-sigma factor, and the alternate sigma factor. However, not enough attention is directed toward studying the role of histidine kinases in the general stress response. Our study identifies the general stress response system in Bartonella henselae, where the gene synteny is conserved and both the PhyR and alternate sigma factor have similar sequence and domain structures with other alpha-proteobacteria. Our data showed that the general stress response genes are up-regulated under conditions that mimic the cat flea vector. Furthermore, we showed that both RpoE and PhyR positively regulate this system and that RpoE also affects transcription of genes encoding heme-binding proteins and the gene encoding the BadA adhesin. Finally, we identified a histidine kinase, annotated as BH13820 that can potentially phosphorylate PhyR.
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. Electronic address: ntu@health.usf.edu.
authors.forename: Nhan
authors.initials: N
authors.lastname: Tu
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. Electronic address: alima@health.usf.edu.
authors.forename: Amorce
authors.initials: A
authors.lastname: Lima
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. Electronic address: zbandeal@health.usf.edu.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bandeali
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. Electronic address: banderso@health.usf.edu.
authors.forename: Burt
authors.initials: B
authors.lastname: Anderson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D018416:Bartonella henselae / Q000502:physiology*; D017124:Conserved Sequence; D042783:Endothelial Cells / Q000378:metabolism / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D005798:Genes, Bacterial; D054730:Protein Interaction Domains and Motifs; D040641:Quantitative Trait Loci; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins
keywords: Alternate sigma factor; Bartonella henselae; Gene regulation; General stress response; Histidine kinase; Two-component system