title: Spatial localization of ryanodine receptors in human cardiac cells.
abstract: We present a novel image processing method to determine the location of ryanodine receptors in cardiac cells. A semi-automatic manual validation by an expert has been used in order to establish the performance of the segmentation method. The approach provides high accuracy under different experimental conditions and it is robust to common sources of noise including experimental, molecular and biological fluctuations.
authors.forename: A
authors.initials: A
authors.lastname: Vallmitjana
authors.forename: C
authors.initials: C
authors.lastname: Nolla
authors.forename: A
authors.initials: A
authors.lastname: Herraiz-Martínez
authors.forename: L
authors.initials: L
authors.lastname: Hove-Madsen
authors.forename: R
authors.initials: R
authors.lastname: Benítez
mesh_terms: D019084:Fluorescent Antibody Technique, Indirect; D006325:Heart Atria / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012372:ROC Curve; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D059010:Single-Cell Analysis
chemical_list: D019837:Ryanodine Receptor Calcium Release Channel