title: Evaluation of antibacterial activity of plant mediated CaO nanoparticles using Cissus quadrangularis extract.
abstract: An assessment of antibacterial activity of greenly synthesized nanoparticles using aqueous stem extract of Cissus quadrangularis was carried out. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, SEM, XRD, FTIR and further subjected for antibacterial activity against the pathogens Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae and Vibrio cholerae. The SEM photograph represents cubic and hexagonal shape of NPs about 58nm respectively whereas the XRD indicated the pure phase of the product and no impurity in peaks of well crystallized products. The FTIR spectrum of nanoparticles showed intensive peaks with blue shift indicating the crystalline and shorten the distance of crystal lattice. The plant mediated CaO nanoparticles showed maximum inhibition on E. coli followed by other strains. In MIC, the plant mediated CaO NPs possess high activity against all the test organisms whereas the CaCl2 and CaO compounds were moderately active. The approach to the synthesis of plant mediated CaO NPs has many advantages as scaled up, economic viability, etc. Application of such ecofriendly nanoparticles in bactericidal, wound healing and other medical applications makes this method potential existing for the large scale synthesis of the inorganic materials.
authors.affiliation: Department of Microbiology, Periyar University, Salem 636011, India. Electronic address: gowdhamimarq@gmail.com.
authors.forename: Gowdhami
authors.initials: G
authors.lastname: Marquis
authors.affiliation: Department of Microbiology, Periyar University, Salem 636011, India; Centre for Nanoscience and Nanotechnology, Periyar University, Salem 636011, India. Electronic address: balaguru@gmail.com.
authors.forename: Balagurunathan
authors.initials: B
authors.lastname: Ramasamy
authors.affiliation: Department of Microbiology, National Institute of Cholera and Enteric Diseases, Kolkata 700010, India. Electronic address: bl_sarkar@hotmail.com.
authors.forename: Sarkar
authors.initials: S
authors.lastname: Banwarilal
authors.affiliation: Department of Microbiology, Periyar University, Salem 636011, India. Electronic address: pmayyasamy@gmail.com.
authors.forename: Ayyasamy Pudukadu
authors.initials: AP
authors.lastname: Munusamy
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry*; D032422:Cissus / Q000737:chemistry* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D017610:Calcium Compounds; D010087:Oxides; D010936:Plant Extracts; C016538:lime
keywords: Antibacterial activity; CaO nanoparticles; Cissus quadrangularis; FTIR; SEM; XRD