title: Cordycepin Suppresses Thymic Stromal Lymphopoietin Expression via Blocking Caspase-1 and Receptor-Interacting Protein 2 Signaling Pathways in Mast Cells.
abstract: Cordycepin (3'-deoxyadenosine) is one of the active components isolated from Cordyceps militaris, and has been shown to have anti-inflammatory, anti-oxidant, anti-aging, and anti-cancer effects. Mast cell-derived thymic stromal lymphopoietin (TSLP) plays an important role in the pathogenesis of allergic inflammatory reactions. Here, we investigated the regulatory effect and mechanisms of cordycepin on the expression of TSLP in the human mast cell line, HMC-1 cells, and in the human keratinocyte cell line, HaCaT cells. Cordycepin significantly decreased the production and mRNA expression of TSLP through the inhibition of caspase-1 and nuclear factor-κB activation. Cordycepin also significantly reduced the phosphorylation of receptor-interacting protein 2 and inhibitory kappa B (IκB) kinase β. Cordycepin significantly decreased the production and mRNA expression of interleukin (IL)-8, IL-1β, IL-6, and tumor necrosis factor-α in activated HMC-1 cells. Moreover, cordycepin significantly decreased the levels of TSLP in activated HaCaT cells. Our studies suggest that cordycepin can be applied to the treatment of allergic inflammatory diseases exacerbated by TSLP.
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University.
authors.forename: Myoung-schook
authors.initials: MS
authors.lastname: Yoou
authors.forename: Mu Hyun
authors.initials: MH
authors.lastname: Jin
authors.forename: So Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Sang Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Byunghyun
authors.initials: B
authors.lastname: Kim
authors.forename: Seok Seon
authors.initials: SS
authors.lastname: Roh
authors.forename: In Hwa
authors.initials: IH
authors.lastname: Choi
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Lee
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D020170:Caspase 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003839:Deoxyadenosines / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051550:I-kappa B Kinase; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects*; D016328:NF-kappa B; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D003839:Deoxyadenosines; D016328:NF-kappa B; D000001:Calcimycin; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D051550:I-kappa B Kinase; D020170:Caspase 1; C058120:cordycepin; D013755:Tetradecanoylphorbol Acetate; D000094632:Thymic Stromal Lymphopoietin