title: The phylogenetic placement of Odontobutis obscura base on complete mitochondrial DNA sequence.
abstract: In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Odontobutis obscura. The aim of our research was to provide some important genetic background information for conservational practice of these valuable fish species. The total length of the mitochondrial genome is 16 864 bp and deposited in the GenBank with accession numbers KT438552. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The nucleotide skewness for the coding strands of O. obscura (GC-skew = -0.26) is biased toward G and the negative GC-skew ranges from -0.51(ND2) to -0.24(CO1). The phylogenetic analysis showed that the Gobiiformes could be divided into three groups, Eleotridae, Odontobutidae and Rhyacichthyidae. The O. Obscura and O. Potamophila are sister species and clustered together.
authors.affiliation: a Hunan Academy of Forestry , Changsha , China.|b Hunan Dongting Lake Wetland Ecosystem Research Station , Changsha , China.
authors.forename: Yandong
authors.initials: Y
authors.lastname: Niu
authors.affiliation: a Hunan Academy of Forestry , Changsha , China.|b Hunan Dongting Lake Wetland Ecosystem Research Station , Changsha , China.
authors.forename: Xiquan
authors.initials: X
authors.lastname: Li
authors.affiliation: a Hunan Academy of Forestry , Changsha , China.|b Hunan Dongting Lake Wetland Ecosystem Research Station , Changsha , China.|c Hunan Cooperation Center of Water Resources Research and Development , Changsha , China.
authors.forename: Canming
authors.initials: C
authors.lastname: Zhang
authors.affiliation: d Forestry Department of Hunan Province , Changsha , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004272:DNA, Mitochondrial; D005399:Fishes / Q000235:genetics*; D050259:Genes, Mitochondrial; D059646:Genome Size; D054629:Genome, Mitochondrial; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: GC-skew; Odontobutis obscura; mitochondrial DNA; phylogenetic analysis