title: Mechanical Integrity of a Decellularized and Laser Drilled Medial Meniscus.
abstract: Since the meniscus has limited capacity to self-repair, creating a long-lasting meniscus replacement may help reduce the incidence of osteoarthritis (OA) after meniscus damage. As a first step toward this goal, this study evaluated the mechanical integrity of a decellularized, laser drilled (LD) meniscus as a potential scaffold for meniscal engineering. To evaluate the decellularization process, 24 porcine menisci were processed such that one half remained native tissue, while the other half was decellularized in sodium dodecyl sulphate (SDS). To evaluate the laser drilling process, 24 additional menisci were decellularized, with one half remaining intact while the other half was LD. Decellularization did not affect the tensile properties, but had significant effects on the cyclic compressive hysteresis and unconfined compressive stress relaxation. Laser drilling decreased the Young's modulus and instantaneous stress during unconfined stress relaxation and the circumferential ultimate strength during tensile testing. However, the losses in mechanical integrity in the LD menisci were generally smaller than the variance observed between samples, and thus, the material properties for the LD tissue remained within a physiological range. In the future, optimization of laser drilling patterns may improve these material properties. Moreover, reseeding the construct with cells may further improve the mechanical properties prior to implantation. As such, this work serves as a proof of concept for generating decellularized, LD menisci scaffolds for the purposes of meniscal engineering.
authors.forename: Emily H
authors.initials: EH
authors.lastname: Lakes
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Matuska
authors.forename: Peter S
authors.initials: PS
authors.lastname: McFetridge
authors.forename: Kyle D
authors.initials: KD
authors.lastname: Allen
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D019245:Compressive Strength; D007834:Lasers; D008422:Materials Testing; D055595:Mechanical Phenomena; D008592:Menisci, Tibial / Q000166:cytology*; D013314:Stress, Mechanical; D013552:Swine