title: Orai1 and STIM1 in ER/PM junctions: roles in pancreatic cell function and dysfunction.
abstract: Membrane contact sites (MCS) are critical junctions that form between the endoplasmic reticulum (ER) and membranes of various organelles, including the plasma membrane (PM). Signaling complexes, including mediators of Ca(2+) signaling, are assembled within MCS, such as the ER/PM junction. This is most evident in polarized epithelial cells, such as pancreatic cells. Core Ca(2+) signaling proteins cluster at the apical pole, the site of inositol 1,4,5-trisphosphate-mediated Ca(2+) release and Orai1/transient receptor potential canonical-mediated store-dependent Ca(2+) entry. Recent advances have characterized the proteins that tether the membranes at MCS and the role of these proteins in modulating physiological and pathological intracellular signaling. This review discusses recent advances in the characterization of Ca(2+) signaling at ER/PM junctions and the relation of these junctions to physiological and pathological Ca(2+) signaling in pancreatic acini.
authors.affiliation: Epithelial Signaling and Transport Section, Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland;
authors.forename: Aran
authors.initials: A
authors.lastname: Son
authors.affiliation: Department of Physiology, School of Medicine, Ewha Womans University, Seoul, Korea;
authors.forename: Seonghee
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Oral Biology, BK 21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: Dong Min
authors.initials: DM
authors.lastname: Shin
authors.affiliation: Epithelial Signaling and Transport Section, Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland; shmuel.muallem@nih.gov.
authors.forename: Shmuel
authors.initials: S
authors.lastname: Muallem
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D008565:Membrane Proteins; D002118:Calcium
keywords: ER; PM; junctions; pancreas; signaling