title: Cellular localization and effects of ectopically expressed hepatitis A virus proteins 2B, 2C, 3A and their intermediates 2BC, 3AB and 3ABC.
abstract: In the course of hepatitis A virus (HAV) infections, the seven nonstructural proteins and their intermediates are barely detectable. Therefore, little is known about their functions and mechanisms of action. Ectopic expression of the presumably membrane-associated proteins 2B, 2C, 3A and their intermediates 2BC, 3AB and 3ABC allowed the intracellular localization of these proteins and their possible function during the replication cycle of HAV to be investigated. In this study, we used rhesus monkey kidney cells, which are commonly used for cell culture experiments, and human liver cells, which are the natural target cells. We detected specific associations of these proteins with distinct membrane compartments and the cytoskeleton, different morphological alterations of the respective structures, and specific effects on cellular functions. Besides comparable findings in both cell lines used with regard to localization and effects of the proteins examined, we also found distinct differences. The data obtained identify so far undocumented interactions with and effects of the HAV proteins investigated on cellular components, which may reflect unknown aspects of the interaction of HAV with the host cell, for example the modification of the ERGIC (ER-Golgi intermediate compartment) structure, an interaction with lipid droplets and lysosomes, and inhibition of the classical secretory pathway.
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Seggewiß
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.
authors.forename: Hedi Verena
authors.initials: HV
authors.lastname: Kruse
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Weilandt
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.|Department of Medicine Huddinge, Karolinska Institutet, Center for Infectious Medicine (CIM), Karolinska University Hospital Huddinge, F59, 141 86, Stockholm, Sweden.
authors.forename: Erna
authors.initials: E
authors.lastname: Domsgen
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Dotzauer
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany. paulmann@uni-bremen.de.
authors.forename: Dajana
authors.initials: D
authors.lastname: Paulmann
mesh_terms: D000818:Animals; D002460:Cell Line; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D000882:Haplorhini; D030041:Hepatitis A virus / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins