title: FSH supplementation to culture medium is beneficial for activation and survival of preantral follicles enclosed in equine ovarian tissue.
abstract: This study investigated the effect of adding different concentrations of bovine recombinant follicle-stimulating hormone on the IVC of equine preantral follicles enclosed in ovarian tissue fragments. Randomized ovarian fragments were fixed immediately (fresh noncultured control) or cultured for 1 or 7 days in α-MEM(+) supplemented with 0, 10, 50, and 100 ng/mL FSH and subsequently analyzed by classical histology. Culture media collected on Day 1 or Day 7 and were analyzed for steroids (estradiol and progesterone) and reactive oxygen species (ROS). After Day 1 and Day 7 of culture, 50-ng/mL FSH treatment had a greater (P < 0.05) percentage of morphologically normal follicles when compared to the other groups, except the 10-ng/mL FSH treatment at Day 1 of culture. The percentage of developing follicles (transition, primary, and secondary), and follicular and oocyte diameters were higher (P < 0.05) in the 50-ng/mL FSH treatment compared to the other groups after Day 7 of culture. Furthermore, estradiol secretion and ROS production were maintained (P > 0.05) throughout the culture in the 50-ng/mL FSH treatment. In conclusion, the addition of 50 ng/mL of FSH promoted activation of primordial follicles to developing follicles, improved survival of preantral follicles, and maintained estradiol and ROS production of equine ovarian tissue after 7 days of culture.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: F L N
authors.initials: FL
authors.lastname: Aguiar
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: F O
authors.initials: FO
authors.lastname: Lunardi
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: R M P
authors.initials: RM
authors.lastname: Rocha
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: J B
authors.initials: JB
authors.lastname: Bruno
authors.affiliation: Postgraduate Biotechnology, Potiguar University/Laureate International Universities, Natal, Rio Grande do Norte, Brazil.
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.affiliation: Federal University of Pampa, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: F W S
authors.initials: FW
authors.lastname: Cibin
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: D C S
authors.initials: DC
authors.lastname: Nunes-Pinheiro
authors.affiliation: Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA.
authors.forename: M O
authors.initials: MO
authors.lastname: Gastal
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
authors.affiliation: Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Apgar
authors.affiliation: Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA. Electronic address: egastal@siu.edu.
authors.forename: E L
authors.initials: EL
authors.lastname: Gastal
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D003470:Culture Media; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006736:Horses; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D027724:Reproductive Techniques, Assisted / Q000662:veterinary; D046509:Tissue Culture Techniques / Q000662:veterinary
chemical_list: D003470:Culture Media; D017382:Reactive Oxygen Species; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone
keywords: Equine ovary; FSH; IVC; Preantral follicle