title: Evaluation of Curcumin Capped Copper Nanoparticles as Possible Inhibitors of Human Breast Cancer Cells and Angiogenesis: a Comparative Study with Native Curcumin.
abstract: Synthesis of metal nanoparticles for improving therapeutic index and drug delivery is coming up as an attractive strategy in the mainstream of cancer therapeutic research. In the present study, curcumin-capped copper nanoparticles (CU-NPs) were evaluated as possible inhibitors of in vivo angiogenesis, pro-angiogenic cytokines involved in promoting tumor angiogenesis along with inhibition of cell proliferation and migration of breast cancer cell line MDA-MB-231. The antiangiogenic potential was assessed using in vivo chorioallantoic membrane (CAM) model. 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)-based cytotoxicity assay was used to assess the effect of CU-NPs against proliferation of breast cancer cell line. The wound healing migration assay was used to evaluate the effects of CU-NPs on the migration ability of breast cancer cell line. Native curcumin (CU) was used as a reference compound for comparison purpose. The result of the present investigation indicates that CU-NPs could not demonstrate impressive antiangiogenic or anticancer activities significantly as compared to native CU. The possible mechanisms of experimental outcomes are discussed in the light of the methods of nanoparticle synthesis in concert with the current state of the art literature.
authors.affiliation: School of Life Sciences, Swami Ramanad Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Kamble
authors.affiliation: National Centre for Cell Sciences, University of Pune Campus, Pune, 411007, MS, India.
authors.forename: Bhimashankar
authors.initials: B
authors.lastname: Utage
authors.affiliation: School of Chemical Sciences, Swami Ramanad Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Pratima
authors.initials: P
authors.lastname: Mogle
authors.affiliation: School of Chemical Sciences, Swami Ramanad Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kamble
authors.affiliation: School of Chemical Sciences, Swami Ramanad Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Hese
authors.affiliation: School of Chemical Sciences, Swami Ramanad Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Dawane
authors.affiliation: School of Life Sciences, Swami Ramanad Teerth Marathwada University, Nanded, 431 606, MS, India. rngacche@rediffmail.com.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Gacche
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage*; D009389:Neovascularization, Pathologic / Q000188:drug therapy*
chemical_list: D020533:Angiogenesis Inhibitors; D004337:Drug Carriers; D003300:Copper; D003474:Curcumin
keywords: angiogenesis; breast cancer; chorioallantoic membrane; curcumin; nanoparticles