title: Suppression of GLTSCR2 expression in renal cell carcinomas.
abstract: Nucleolar protein PICT-1/GLTSCR2 (GLTSCR2) has both tumor suppressive and oncogenic activities, depending on the types of cancer tissue and its expression level. The role of GLTSCR2 in renal cell carcinoma (RCC) has not yet been addressed. The aims of this study were to evaluate GLTSCR2 expression in RCC tissue and to determine pathological significance of GLTSCR2 in terms of tumor grade. RCC and adjacent normal tissue from 84 different patients was retrieved from nephrectomy specimens. The expression level of GLTSCR2 in RCC tissues was determined via immunohistochemical staining and invasion was determined using transwell chambers with Matrigel-coated membranes. The expression of GLTSCR2 was suppressed in about 80% of the carcinoma specimens compared to noncancerous renal tissue and inversely correlated with Fuhrman nuclear grade (r=-0.40, p<0.05). Knockdown of GLTSCR2 expression increased the invasiveness of SNU267 RCC cells. The expression of GLTSCR2 was suppressed in RCCs and its downregulation accentuated the malignant phenotype.
authors.affiliation: Department of Pathology, College of Medicine, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Cho
authors.affiliation: Department of Urology, College of Medicine, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Hyung-Lae
authors.initials: HL
authors.lastname: Lee
authors.affiliation: Department of Pathology, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, 134-727, Korea.
authors.forename: Sung-Jig
authors.initials: SJ
authors.lastname: Lim
authors.affiliation: Department of Pathology, College of Medicine, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Youn-Wha
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Department of Preventive Medicine, College of Medicine, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Choe
authors.affiliation: Department of Pathology, College of Medicine, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Sun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Pathology, College of Medicine, Kyung Hee University, Seoul, 130-701, Korea. Electronic address: jhpark@khu.ac.kr.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D010641:Phenotype; D034622:RNA Interference; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C500712:NOP53 protein, human; D025521:Tumor Suppressor Proteins
keywords: Glioblastoma tumor suppressive candidate region gene 2; Immunohistochemistry; Invasion; Renal cell carcinoma