title: [Effect of Toll-like receptor 4 signaling activation on the biological behavior of gastric cancer cell lines].
abstract: OBJECTIVE
  To investigate the effects of Toll like receptor (TLR) signaling activation on the malignant biological behavior of gastric cancer cell lines.
  
  
  METHODS
  RT-PCR was employed to detect the basal expression of TLRs in the gastric cancer cell lines. According to the basal RNA expression, the proteic expression of TLR4 of the cell lines was examined using Western blot analysis. BGC823 and AGS cell lines were chosen for investigating the effects of TLR4 signaling activation on the biological behavior due to the high level of TLR4 proteic expression.
  
  
  RESULTS
  TLR4 RNA expression was higher than any other TLRs in the gastric cancer cell lines. Higher relative proteic expression of TLR4 was observed in BGC823 (1. 41 ± 0. 05) and AGS (1. 22 ± 0. 05) (all P <0. 05). Activation of the TLR4 signaling pathway by LPS increased proliferation, migration and invasion of BGC823 and AGS cell lines (all P < 0. 05).
  
  
  CONCLUSIONS
  TLR4 may participate in the metastasis of gastric cancer through promotes the malignant biological behavior of gastric cancer cell, and may be a potential therapeutic target.
authors.forename: Nina
authors.initials: N
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: An
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Guibin
authors.initials: G
authors.lastname: Yang
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D006801:Humans; D015398:Signal Transduction; D013274:Stomach Neoplasms; D051197:Toll-Like Receptor 4
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4