title: Real-time PCR validation of visually identified snapper Chrysophrys auratus (Sparidae) eggs.
abstract: In this study, a total of 212 eggs were visually identified as snapper Chrysophrys auratus. Real-time PCR confirmed visual identification in 69% of cases but corroboration varied widely among plankton samples. The use of molecular tools to support visual identification prior to adopting daily egg production stock assessment methods should be considered.
authors.affiliation: Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.|School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Dias
authors.affiliation: Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.
authors.forename: C B
authors.initials: CB
authors.lastname: Wakefield
authors.affiliation: Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.
authors.forename: D V
authors.initials: DV
authors.lastname: Fairclough
authors.affiliation: Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Jackson
authors.affiliation: Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Travers
authors.affiliation: Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Snow
mesh_terms: D000818:Animals; D001315:Australia; D018533:Biomass; D010063:Ovum / Q000145:classification*; D010473:Perciformes / Q000145:classification* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
keywords: Pagrus; daily egg production method; fisheries; spawning aggregation; spawning stock biomass