title: [Effects of Paris polyphylla saponin VII plus silica nano composite on ovarian cancer drug resistance in vitro].
abstract: OBJECTIVE
  To explore the drug resistance of Chonglou saponin VII plus silica nano complex for ovarian cancer.
  
  
  METHODS
  The method of thiazolyl blue tetrazolium blue (MTT) was employed for determining the proliferation inhibition rate of Chonglou saponin VII plus silica nano composite for SKOV3/DDP drug-resistant human ovarian cancer cell. Flow cytometry and Hoechst 33342 staining were used for detecting apoptosis rate and cell cycle phase distribution. And the changes of apoptosis-related protein were examined by immunoblot assay.
  
  
  RESULTS
  There were obvious inhibitory effects of Chonglou saponin VII on cell proliferation. The inhibition rate increased with rising drug concentration with an obvious dose-effect relationship (P < 0.05). At the same time, the cell proliferation inhibition rate significantly improved at 48 h versus 24 h (P < 0.05). Flow cytometry and Hoechst 33342 staining showed that, after a 24 h treatment of SKOV3/DDP cells with 50 and 100 µmol/L Chonglou saponin VII, early apoptotic cell ratio significantly increased compared with control group.
  
  
  CONCLUSIONS
  The composite of Paris polyphylla saponin VII and silica nano has obvious proliferation inhibitory effects on SKOV3/DDP cells. And there is a significant concentration dependence and it also has some correlation with time. The above composite can increased the proportion of early apoptotic cell ratio.
authors.affiliation: Department of Obstetrics & Gynecology, Second Hospital, Jilin University, Changchun 130041, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
authors.forename: Jialing
authors.initials: J
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Email: fanlimei1971@126.com.
authors.forename: Limei
authors.initials: L
authors.lastname: Fan
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D049329:Nanostructures; D010051:Ovarian Neoplasms; D010297:Paris; D012503:Saponins; D012822:Silicon Dioxide
chemical_list: D012503:Saponins; D012822:Silicon Dioxide