title: MicroRNA-542-3p suppresses cellular proliferation of bladder cancer cells through post-transcriptionally regulating survivin.
abstract: OBJECTIVE
  To investigate the clinical significance of microRNA-542-3p (miR-542-3p) and its target gene survivin in human bladder cancer, and to determine their functions in malignant phenotypes of this disease.
  
  
  METHODS
  Expression levels of miR-542-3p and survivin mRNA in bladder cancer and adjacent normal tissues were detected by quantitative RT-PCR. Cell proliferation and cell cycle were assessed by cell viability assay, flow cytometry and colony formation assay based on two human bladder cancer cell lines.
  
  
  RESULTS
  MiR-542-3p expression was downregulated, while survivin mRNA expression was upregulated in bladder cancer tissues, compared to adjacent normal tissues (both P<0.001). Importantly, the expression level of miR-542-3p in bladder cancer tissues was negatively correlated with that of survivin mRNA. MiR-542-3p-low and/or survivin-high expression were all significantly associated with tumor stage (all P<0.05) and tumor recurrence (all P<0.05) of patients with bladder cancer. Moreover, the enforced expression of miR-542-3p remarkably inhibited cell proliferation by inducing G1 phase arrest in both T24 and J82 cells, and decreased the expression level of survivin protein. In contrast, the knock-down of miR-542-3p dramatically promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression level of survivin protein.
  
  
  CONCLUSIONS
  MiR-542-3p and its target gene survivin may play crucial roles in the aggressive progression of human bladder cancer. More interestingly, miR-542-3p may function as a tumor suppressor and inhibit the proliferation of bladder cancer cells, implying that miR-542-3p-survivin signal axis might be a novel therapeutic target of this disease.
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Medical Examination of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xue
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China.
authors.forename: Changyuan
authors.initials: C
authors.lastname: Dai
authors.affiliation: Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, China. Electronic address: 13865059474@163.com.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis*; D000077022:Survivin; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C572357:MIRN542 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D000077022:Survivin
keywords: Bladder cancer; Cell proliferation; Clinicopathological characteristics; MicroRNA-542-3p; Survivin