title: Calcium Signaling Is Required for Erythroid Enucleation.
abstract: Although erythroid enucleation, the property of erythroblasts to expel their nucleus, has been known for 7ore than a century, surprisingly little is known regarding the molecular mechanisms governing this unique developmental process. Here we show that similar to cytokinesis, nuclear extrusion requires intracellular calcium signaling and signal transduction through the calmodulin (CaM) pathway. However, in contrast to cytokinesis we found that orthochromatic erythroblasts require uptake of extracellular calcium to enucleate. Together these functional studies highlight a critical role for calcium signaling in the regulation of erythroid enucleation.
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Christina B
authors.initials: CB
authors.lastname: Wölwer
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Luke B
authors.initials: LB
authors.lastname: Pase
authors.affiliation: Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.|Immune Signaling Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.|Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Melbourne, Victoria, Australia.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Russell
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.|Department of Pathology, University of Melbourne, Melbourne, Victoria, Australia.|Department of Biochemistry and Molecular Biology, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Patrick O
authors.initials: PO
authors.lastname: Humbert
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D002147:Calmodulin / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004900:Erythroblasts / Q000378:metabolism*; D004920:Erythropoiesis / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D002147:Calmodulin; D002118:Calcium