title: RNA Binding Proteins in the miRNA Pathway.
abstract: microRNAs (miRNAs) are short ~22 nucleotides (nt) ribonucleic acids which post-transcriptionally regulate gene expression. miRNAs are key regulators of all cellular processes, and the correct expression of miRNAs in an organism is crucial for proper development and cellular function. As a result, the miRNA biogenesis pathway is highly regulated. In this review, we outline the basic steps of miRNA biogenesis and miRNA mediated gene regulation focusing on the role of RNA binding proteins (RBPs). We also describe multiple mechanisms that regulate the canonical miRNA pathway, which depends on a wide range of RBPs. Moreover, we hypothesise that the interaction between miRNA regulation and RBPs is potentially more widespread based on the analysis of available high-throughput datasets.
authors.affiliation: Faculty of Science 1, University of Technology, Sydney, NSW 2007, Australia. patrick.connerty@student.uts.edu.au.
authors.forename: Patrick
authors.initials: P
authors.lastname: Connerty
authors.affiliation: Faculty of Engineering and Information technology 2, University of Technology, Sydney, NSW 2007, Australia. alireza.ahadi@uts.edu.au.
authors.forename: Alireza
authors.initials: A
authors.lastname: Ahadi
authors.affiliation: Faculty of Engineering and Information technology 2, University of Technology, Sydney, NSW 2007, Australia. gyorgy.hutvagner@uts.edu.au.
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Hutvagner
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism*; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D043244:Ribonuclease III / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D035683:MicroRNAs; D043244:Ribonuclease III
keywords: Argonaute; Dicer; Drosha; RNA binding protein; cross-linking and immonoprecipitation (CLIP); miRNA