title: Serum metabolomics study of Traditional Chinese medicine formula intervention to polycystic ovary syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a most common, heterogeneous, complex endocrinopathy disease. Traditional Chinese medicine (TCM) has been used in the treatment of PCOS for many years. However, the mechanism underlying TCM remains obscure and challenging. In this study, 30 PCOS subjects were separated into normoinsulinemic group (NI=13) and hyperinsulinemic group (HI=17), and treated for three menstrual cycles with TCM Formula, Bushen Huatan Formula (BHF). A metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) coupled with linear ion trap Orbi-trap mass spectrometer (LTQ Orbi-trap MS) is used to investigate serum metabolic changes of TCM intervention to PCOS. After BHF intervention for three menstrual cycles, the serum levels of glycerophosphorylethanolamine (GPEA), creatine, creatinine decreased in both NI and HI groups. Furthermore, in NI group, the main manifestation was the changes of phospholipid metabolism. While in HI group, lysine, phenol sulfate, phe-phe etc. decreased, and ornithine, proline, betaine, acetylcholine etc. increased. Combined with clinical biochemical data, BHF was proved effective to PCOS by reducing the inflammatory reaction and oxidative stress. This study also illustrates that the LC-MS based metabolomic approach is a helpful tool to evaluate curative effect and to understand the mechanisms of TCM.
authors.affiliation: Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Lu
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xj_zhao1@126.com.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Chengkun
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China. Electronic address: houlihui2007@sina.com.
authors.forename: Lihui
authors.initials: L
authors.lastname: Hou
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects; D013058:Mass Spectrometry / Q000379:methods; D008516:Medicine, Chinese Traditional / Q000379:methods; D055432:Metabolomics / Q000379:methods; D018384:Oxidative Stress / Q000187:drug effects; D010743:Phospholipids / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*; D055815:Young Adult
chemical_list: D004365:Drugs, Chinese Herbal; D010743:Phospholipids
keywords: Insulin resistance; LC–MS; Metabolomics; Polycystic ovary syndrome; Traditional Chinese medicine