title: Apoptosis-related microRNA changes in the right atrium induced by remote ischemic perconditioning during valve replacement surgery.
abstract: We previously found that remote ischemic perconditioning (RIPerc) was effective in attenuating myocardial injury during cardiac surgery. Given that microRNAs (miRs) act as an important player in ischemic/reperfusion (I/R) injury and apoptosis, this study aimed to investigate whether RIPerc reduces apoptosis in atrial myocardium and which apoptosis-related miRs are involved during valve replacement surgery. Here, we demonstrated that RIPerc inhibited apoptosis in atrial myocardium during cardiac ischemia and that 17 miRs showed at least a 1.5-fold change in expression after ischemia. Of the 17 miRs, 9 miRs, including miR-1, miR-21, miR-24, and miR-195, which are related to apoptosis, exhibited different expression patterns in the RIPerc group compared with the control. Using qRT-PCR and Western blotting, we demonstrated that miR-1 and miR-195 were downregulated and that their common putative target gene Bcl-2 was upregulated in the RIPerc group. However, the differences in miR-21 and miR-24 expression, together with programmed cell death 4 (PDCD4), which is the target gene of miR-21, were not significant. These findings provide some insight into the role of miRs in the cardioprotective effects induced by RIPerc.
authors.affiliation: Department of cardiovascular surgery, Xiangya Hospital, Central-South University, Changsha, Hunan, China, 410078.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of cardiovascular surgery, Xiangya Hospital, Central-South University, Changsha, Hunan, China, 410078.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of cardiovascular surgery, Xiangya Hospital, Central-South University, Changsha, Hunan, China, 410078.
authors.forename: Lingjin
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of cardiovascular surgery, Xiangya Hospital, Central-South University, Changsha, Hunan, China, 410078.
authors.forename: Rimao
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of cardiovascular surgery, Xiangya Hospital, Central-South University, Changsha, Hunan, China, 410078.
authors.forename: Ri
authors.initials: R
authors.lastname: Chen
mesh_terms: D000328:Adult; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006325:Heart Atria / Q000378:metabolism*; D006349:Heart Valve Diseases / Q000378:metabolism / Q000601:surgery*; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism; D034622:RNA Interference; D016896:Treatment Outcome
chemical_list: D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs