title: Waste cooking oil: A new substrate for carotene production by Blakeslea trispora in submerged fermentation.
abstract: The objective of this study was to evaluate a waste, waste cooking oil (WCO) as substrate for carotene production by Blakeslea trispora in shake flask culture. WCO was found to be a useful substrate for carotene production. B. trispora formed only pellets during fermentation. The oxidative stress in B. trispora induced by hydroperoxides and BHT as evidenced by increase of the specific activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly the production of carotenes. The highest concentration of carotenes (2021 ± 75 mg/l or 49.3 ± 0.2 mg/g dry biomass) was obtained in culture grown in WCO (50.0 g/l) supplemented with CSL (80.0 g/l) and BHT (4.0 g/l). In this case the carotenes produced consisted of β-carotene (74.2%), γ-carotene (23.2%), and lycopene (2.6%). The external addition in the above medium glucose, Span 80, yeast extract, casein acid hydrolysate, l-asparagine, thiamine. HCl, KH2PO4, and MgSO4·7H2O did not improve the production of carotenes.
authors.affiliation: Laboratory of Food Engineering and Processing, Department of Food Science and Technology, Aristotle University, Box 250, 54124 Thessaloniki, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Nanou
authors.affiliation: Laboratory of Food Engineering and Processing, Department of Food Science and Technology, Aristotle University, Box 250, 54124 Thessaloniki, Greece. Electronic address: roukas@agro.auth.gr.
authors.forename: Triantafyllos
authors.initials: T
authors.lastname: Roukas
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D002374:Catalase / Q000378:metabolism; D003296:Cooking; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation / Q000502:physiology; D000077276:Lycopene; D009090:Mucorales / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014865:Waste Disposal, Fluid; D019207:beta Carotene / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D004042:Dietary Fats, Unsaturated; D019207:beta Carotene; D002338:Carotenoids; D002374:Catalase; D013482:Superoxide Dismutase; D000077276:Lycopene
keywords: Blakeslea trispora; Carotenes; Culture morphology; Oxidative stress; Waste cooking oil