title: LAPONITE®-stabilized iron oxide nanoparticles for in vivo MR imaging of tumors.
abstract: We report the synthesis, characterization and utilization of LAPONITE®-stabilized magnetic iron oxide nanoparticles (LAP-Fe3O4 NPs) as a high performance contrast agent for in vivo magnetic resonance (MR) detection of tumors. In this study, Fe3O4 NPs were synthesized by a facile controlled coprecipitation route in LAP solution, and the formed LAP-Fe3O4 NPs have great colloidal stability and about 2-fold increase of T2 relaxivity than Fe3O4 NPs (from 247.6 mM(-1) s(-1) to 475.9 mM(-1) s(-1)). Moreover, cytotoxicity assay and cell morphology observation demonstrate that LAP-Fe3O4 NPs display good biocompatibility in the given Fe concentration range, and in vivo biodistribution results prove that NPs can be metabolized and cleared out of the body. Most importantly, LAP-Fe3O4 NPs can not only be used as a contrast agent for MR imaging of cancer cells in vitro due to the effective uptake by tumor cells, but also significantly enhance the contrast of a xenografted tumor model. Therefore, the developed LAP-based Fe3O4 NPs with good colloidal stability and exceptionally high transverse relaxivity may have tremendous potential in MR imaging applications.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Ding
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Yilun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Radiology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, People's Republic of China. cpengrr@gmail.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Peng
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn and State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, People's Republic of China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. ruiguo@dhu.edu.cn xshi@dhu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
mesh_terms: D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008280:Magnetics; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D017640:Silicates / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D017640:Silicates; C000499:ferric oxide; D011094:Polyethyleneimine; C524813:laponite