title: Molecular detection of Porcine astrovirus in Sichuan Province, China.
abstract: BACKGROUND
  Porcine astrovirus (PoAstV) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (PoAstV1-PoAstV5) within the genus Mamastrovirus. However, our knowledge of the diversity and epidemiology of PoAstV in China is limited.
  
  
  RESULTS
  In this study, fecal samples from 21/120 (17.5 %) domestic pigs, including 18/100 (18 %) diarrheic and 3/20 (15 %) healthy pigs, and from 1/9 (11.1 %) healthy wild boars tested in Sichuan Province were positive for PoAstV on reverse transcription-PCR. Of the 22 positive samples, 13.6 % were positive for PoAstV only, whereas 40.9 % also contained Porcine epidemic diarrhea virus (PEDV), 22.7 % also contained porcine group A rotavirus (PRoVA), and 22.7 % also contained PEDV and PRoVA. A phylogenetic analysis of the RdRp gene revealed genetic heterogeneity among the PoAstV sequences and two lineages were detected in this study, with PoAstV-2 predominant. PoAstV-5 was detected in wild boars for the first time.
  
  
  CONCLUSIONS
  PoAstV infections exist in Sichuan Province regardless of the disease status in the pig population, either alone or in combination with other enteric viruses, and may be associated with diarrhea.
authors.affiliation: Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Veterinary Biologicals engineering and technology Research Center of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Institute of Animal Nutrition, Sichuan Academy of Animal Science, Chengdu, 610299, China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Veterinary Biologicals engineering and technology Research Center of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Institute of Animal Nutrition, Sichuan Academy of Animal Science, Chengdu, 610299, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Yin
authors.affiliation: Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China. abtczyc@163.com.|Veterinary Biologicals engineering and technology Research Center of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China. abtczyc@163.com.|Institute of Animal Nutrition, Sichuan Academy of Animal Science, Chengdu, 610299, China. abtczyc@163.com.
authors.forename: Yuanchen
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Veterinary Biologicals engineering and technology Research Center of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Institute of Animal Nutrition, Sichuan Academy of Animal Science, Chengdu, 610299, China.
authors.forename: Bi
authors.initials: B
authors.lastname: Li
authors.affiliation: Livestock and Poultry Biological Products Key Laboratory of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.|Veterinary Biologicals engineering and technology Research Center of Sichuan Province, Huashen Veterinary Biological Products Co., LTD, Chengdu, 610299, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Ai
authors.affiliation: Institute of Animal Nutrition, Sichuan Academy of Animal Science, Chengdu, 610299, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Pan
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611134, China.
authors.forename: Wanzhu
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D019350:Astroviridae Infections / Q000662:veterinary; D002681:China; D005243:Feces / Q000821:virology; D001257:Mamastrovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D012367:RNA, Viral