title: First Evidence of a Hybrid of Leishmania (Viannia) braziliensis/L. (V.) peruviana DNA Detected from the Phlebotomine Sand Fly Lutzomyia tejadai in Peru.
abstract: The natural infection of sand flies by Leishmania was examined in the Department of Huanuco of Peru, where cutaneous leishmaniasis caused by a hybrid of Leishmania (Viannia) braziliensis/L. (V.) peruviana is endemic. A total of 2,997 female sand flies were captured by CDC light traps and Shannon traps, of which 2,931 and 66 flies were identified as Lutzomyia tejadai and Lu fischeri, respectively. Using crude DNA extracted from individual sand flies as a template, Leishmania DNA was detected from one Lu. tejadai. The parasite species was identified as a hybrid of L. (V.) braziliensis/L. (V.) peruviana on the basis of cytochrome b and mannose phosphate isomerase gene analyses. The result suggested that Lu. tejadai is responsible for the transmission of the hybrid Leishmania circulating in this area.
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Kato
authors.affiliation: Sección de Entomología, Instituto de Medicina Tropical "Daniel A. Carrión" y Departamento Académico de Microbiología Médica, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Peru.|Laboratorio de Entomología, Instituto Nacional de Salud, Lima, Peru.
authors.forename: Abraham G
authors.initials: AG
authors.lastname: Cáceres
authors.affiliation: Centro de Biomedicina, Facultad de Medicina, Universidad Central del Ecuador, Quito, Ecuador.|Prometeo, Secretaría Nacional de Educacion Superior, Ciencia, Tecnologia e Innovacion (SENESCYT), Quito, Ecuador.|Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hashiguchi
mesh_terms: D000818:Animals; D005260:Female; D006824:Hybridization, Genetic; D007891:Leishmania / Q000145:classification / Q000235:genetics*; D010568:Peru; D010802:Phylogeny; D011576:Psychodidae / Q000469:parasitology*