title: Novel Polyanions Inhibiting Replication of Influenza Viruses.
abstract: Novel sulfonated derivatives of poly(allylamine hydrochloride) (NSPAHs) and N-sulfonated chitosan (NSCH) have been synthesized, and their activity against influenza A and B viruses has been studied and compared with that of a series of carrageenans, marine polysaccharides of well-documented anti-influenza activity. NSPAHs were found to be nontoxic and very soluble in water, in contrast to gel-forming and thus generally poorly soluble carrageenans.In vitroandex vivostudies using susceptible cells (Madin-Darby canine kidney epithelial cells and fully differentiated human airway epithelial cultures) demonstrated the antiviral effectiveness of NSPAHs. The activity of NSPAHs was proportional to the molecular mass of the chain and the degree of substitution of amino groups with sulfonate groups. Mechanistic studies showed that the NSPAHs and carrageenans inhibit influenza A and B virus assembly in the cell.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Krakow, Poland Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Ciejka
authors.affiliation: Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Milewska
authors.affiliation: AGH University of Science and Technology, Academic Centre for Materials and Nanotechnology, Krakow, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wytrwal
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Zabrze, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Wojarski
authors.affiliation: Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Golda
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Zabrze, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Ochman
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Krakow, Poland szczubia@chemia.uj.edu.pl k.a.pyrc@uj.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szczubialka
authors.affiliation: Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland szczubia@chemia.uj.edu.pl k.a.pyrc@uj.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pyrc
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D048271:Chitosan / Q000138:chemical synthesis / Q000494:pharmacology*; D004285:Dogs; D004847:Epithelial Cells / Q000187:drug effects / Q000821:virology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D009981:Influenza B virus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D020128:Inhibitory Concentration 50; D061985:Madin Darby Canine Kidney Cells; D011073:Polyamines / Q000138:chemical synthesis / Q000494:pharmacology*; D000071228:Polyelectrolytes; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology*; D012367:RNA, Viral / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013329:Structure-Activity Relationship; D013463:Sulfuric Acid Esters / Q000138:chemical synthesis / Q000494:pharmacology*; D019065:Virus Assembly / Q000187:drug effects; D053585:Virus Attachment / Q000187:drug effects; D038301:Virus Inactivation / Q000187:drug effects; D053586:Virus Internalization / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D048271:Chitosan; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D012367:RNA, Viral; D013463:Sulfuric Acid Esters; C063994:polyallylamine