title: Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi.
abstract: The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.
authors.affiliation: Department of Soil Science, North Carolina State University, Raleigh, NC 27695-7619, United States.
authors.forename: Megan Y
authors.initials: MY
authors.lastname: Andrews
authors.affiliation: University of Minnesota, Department of Earth Sciences, Minneapolis, MN 55455-0231, United States.
authors.forename: Cara M
authors.initials: CM
authors.lastname: Santelli
authors.affiliation: Department of Soil Science, North Carolina State University, Raleigh, NC 27695-7619, United States. Electronic address: owen_duckworth@ncsu.edu.
authors.forename: Owen W
authors.initials: OW
authors.lastname: Duckworth
mesh_terms: D000362:Agar / Q000737:chemistry; D003470:Culture Media; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D008828:Microbiological Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods*; D017262:Siderophores / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D017262:Siderophores; C015076:chrome azurol S; D000362:Agar
keywords: CAS assay; Fungi; Quantitative assay; Siderophores