title: Simultaneous Drug and Gene Delivery from the Biodegradable Poly(ε-caprolactone) Nanofibers for the Treatment of Liver Cancer.
abstract: In this study, we present anti-cancer drug containing nanofiber-mediated gene delivery to treat liver cancer. Electro-spun nanofibers have big potential for local delivery and sustained release of therapeutic gene and drugs. We reported a temperature-responsive nanofibers mainly compounded by branched poly(ε-caprolactone) (PCL) macro-monomers and anti-cancer drug paclitaxel. The nanofiber could be administrated into liver tumors to dramatically hinder their growth and prevent their metastasis. As a result, paclitaxel encapsulated PCL (PTX/PCL) nanofibers with diameters of around several tens nanometers to 10 nm were successfully obtained by electro-spinning and observed in scanning electron microscopy (SEM). Nanoparticles composed of disulfide cross-linked branched PEI (ssPEI) and anti-cancer therapeutic gene miRNA-145 were complexed based on the electrostatic interaction and coated over the paclitaxel-loaded nanofiber. MicroRNA 145/ssPEI nanoparticles (MSNs) immobilized on the PTX/PCL nanofiber showed time-dependent sustained release of the microRNA for enhanced uptake in neighboring liver cancer cells without any noticeable cytotoxicity. From this study we are expecting a synergistic effect on the cancer cell suppression since we have combined the drug and gene delivery. This approach uses the nanofibers and nanoparticles together for the treatment of cancer and the detailed investigation in vitro and in vivo must be conducted for the practicality of this study. The polymer is biodegradable and the toxicity issues must be cleared by our approach.
authors.forename: Hui-Lian
authors.initials: HL
authors.lastname: Che
authors.forename: Hwa Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Koichiro
authors.initials: K
authors.lastname: Uto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ebara
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Kim
authors.forename: Takao
authors.initials: T
authors.lastname: Aoyagi
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
mesh_terms: D016503:Drug Delivery Systems / Q000379:methods*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics; D057139:Nanofibers / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D011091:Polyesters; C016240:polycaprolactone; D017239:Paclitaxel