title: Methods for studying RNA localization in bacteria.
abstract: The subcellular localization of RNA transcripts provides important insights into biological processes. Hence, understanding the mechanisms underlying RNA targeting is a high priority aim of modern cell biology. The advancements in imaging techniques, such as in situ hybridization and live-cell imaging, coupled with the evolution in optical microscopy led to the discovery that bacterial RNAs, despite the lack of nucleus, are specifically localized. Here we describe the methods used to study RNA localization in bacteria and their applications and discuss their advantages and limitations.
authors.affiliation: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University - Faculty of Medicine, P.O. Box 12272, Jerusalem 91120, Israel.
authors.forename: Shanmugapriya
authors.initials: S
authors.lastname: Kannaiah
authors.affiliation: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University - Faculty of Medicine, P.O. Box 12272, Jerusalem 91120, Israel. Electronic address: ornaam@ekmd.huji.ac.il.
authors.forename: Orna
authors.initials: O
authors.lastname: Amster-Choder
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001419:Bacteria / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: Bacterial cell; Fluorescence microscopy; Imaging techniques; Quenching; RNA localization; Super-resolution microscopy