title: Simultaneous Chiral Separation of Flavanone, Naringenin, and Hesperetin Enantiomers by RP-UHPLC-DAD.
abstract: A rapid and effective RP-UHPLC-DAD method for enantioseparation of three flavanones, i.e., flavanone, naringenin, and hesperetin, was developed and validated. Chromatographic separation of the analytes was performed using a Chiralpak AD-3R analytical column under reverse phase conditions with methanol as the mobile phase. The method was validated in the concentration range of 0.2 to 50 µg/mL for enantiomers of flavanone and 0.5 to 50 µg/mL for enantiomers of naringenin and hesperetin. The limits of quantification were between 0.03 to 0.5 µg/mL. Intraday and interday precision were below 14% and accuracy varied from 0.04 to 8.17%.
authors.affiliation: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Baranowska
authors.affiliation: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland.
authors.forename: Judyta
authors.initials: J
authors.lastname: Hejniak
authors.affiliation: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Magiera
mesh_terms: D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000737:chemistry*; D006569:Hesperidin / Q000737:chemistry*; D015394:Molecular Structure; D048448:Phenylcarbamates / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D044950:Flavanones; D048448:Phenylcarbamates; C080033:Chiralpak AD; D000688:Amylose; D006569:Hesperidin; C005273:naringenin; C013015:hesperetin; C028610:flavanone
keywords: chromatography; enantiomers; flavonoids