title: Biomimetic Mineralized Hierarchical Graphene Oxide/Chitosan Scaffolds with Adsorbability for Immobilization of Nanoparticles for Biomedical Applications.
abstract: Biomimetic calcium phosphate mineralized graphene oxide/chitosan (GO/CS) scaffolds with hierarchical structures were developed. First, GO/CS scaffolds with large micropores (∼300 μm) showed high mechanical strength due to the electrostatic interaction between the oxygen-containing functional groups of GO and the amine groups of CS. Second, octacalcuim phosphate (OCP) with porous structures (∼1 μm) was biomimetically mineralized on the surfaces of the GO/CS scaffolds (OCP-GO/CS). The hierarchical microporous structures of OCP-GO/CS scaffolds provide a suitable environment for cell adhesion and growth. The scaffolds have exceptional adsorbability of nanoparticles. Bone morphogenetic protein-2 (BMP-2)-encapsulated bovine serum albumin (BSA) nanoparticles and Ag nanoparticles (Ag-NPs) were adsorbed in the scaffolds for enhancement of osteoinductivity and antibacterial properties, respectively. Antibacterial tests showed that the scaffolds exhibited high antibacterial properties against both Escherichia coli and Staphylococcus epidermidis. In vitro and in vivo experiments revealed that the scaffolds have good biocompatibility, enhanced bone marrow stromal cells proliferation and differentiation, and induced bone tissue regeneration. Thus, the biomimetic OCP-GO/CS scaffolds with immobilized growth factors and antibacterial agents might be excellent candidates for bone tissue engineering.
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, Sichuan 610031, China.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Xie
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, Sichuan 610031, China.|National Engineering Research Center for Biomaterials, Genome Research Center for Biomaterials, Sichuan University , Chengdu, Sichuan 610064, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Lu
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, Sichuan 610031, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, Sichuan 610031, China.
authors.forename: Jielong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, Sichuan 610031, China.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, Sichuan 610031, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.affiliation: National Engineering Research Center for Biomaterials, Genome Research Center for Biomaterials, Sichuan University , Chengdu, Sichuan 610064, China.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, Southwest University of Science and Technology , Mianyang, Sichuan 621000, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Materials Science and Engineering, South University of Science and Technology , Shenzhen, Guangdong 518055, China.
authors.forename: Fuzeng
authors.initials: F
authors.lastname: Ren
authors.affiliation: Centre for NanoScale Science and Technology, School of Computer Science, Engineering and Mathematics, Flinders University , Tonsley, South Australia 5042, Australia.
authors.forename: Youhong
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D020811:Biomedical Technology / Q000379:methods*; D040761:Biomimetic Materials / Q000737:chemistry*; D002130:Calcium Phosphates / Q000494:pharmacology; D048430:Cell Shape; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008826:Microbial Sensitivity Tests; D008903:Minerals / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000473:pathology; D012996:Solutions; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D008903:Minerals; D012996:Solutions; C022045:octacalcium phosphate; D006108:Graphite; D048271:Chitosan
keywords: adsorbability; antibacterial property; biomimetic scaffold; graphene oxide; hierarchical structure; osteoinductivity