title: Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification.
abstract: Gene targeting via homologous recombination, albeit highly inefficient in human cells, is considered a powerful tool for analyzing gene functions. Despite recent progress in the application of artificial nucleases for genome editing, safety issues remain a concern, particularly when genetic modification is used for therapeutic purposes. Therefore, the development of gene-targeting vectors is necessary for safe and sophisticated genetic modification. In this paper, we describe the effect of vector structure on random integration, which is a major obstacle in efficient gene targeting. In addition, we focus on the features of exon-trapping-type gene-targeting vectors, and discuss a novel strategy for negative selection to enhance gene targeting in human cells.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University.
authors.forename: Shinta
authors.initials: S
authors.lastname: Saito
authors.forename: Noritaka
authors.initials: N
authors.lastname: Adachi
mesh_terms: D005786:Gene Expression Regulation / Q000502:physiology*; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans