title: Nitrogen-doped carbon nanoparticle modulated turn-on fluorescent probes for histidine detection and its imaging in living cells.
abstract: In this work, nitrogen-doped carbon nanoparticle (N-CNP) modulated turn-on fluorescent probes were developed for rapid and selective detection of histidine. The as synthesized N-CNPs exhibited high fluorescence quantum yield and excellent biocompatibility. The fluorescence of N-CNPs can be quenched selectively by Cu(II) ions with high efficiency, and restored by the addition of histidine owing to the competitive binding of Cu(II) ions and histidine that removes Cu(II) ions from the surface of the N-CNPs. Under the optimal conditions, a linear relationship between the increased fluorescence intensity of N-CNP/Cu(II) ion conjugates and the concentration of histidine was established in the range from 0.5 to 60 μM. The detection limit was as low as 150 nM (signal-to-noise ratio of 3). In addition, the as-prepared N-CNP/Cu(II) ion nanoprobes showed excellent biocompatibility and were applied for a histidine imaging assay in living cells, which presented great potential in the bio-labeling assay and clinical diagnostic applications.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China. niezhou.hnu@gmail.com and Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China. liu-yang@mail.tsinghua.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China. liu-yang@mail.tsinghua.edu.cn.
authors.forename: Tingbi
authors.initials: T
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China. niezhou.hnu@gmail.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Miao
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China. liu-yang@mail.tsinghua.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China. niezhou.hnu@gmail.com.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D002244:Carbon / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006639:Histidine / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009584:Nitrogen; D061848:Optical Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D006639:Histidine; D002244:Carbon; D003300:Copper; D009584:Nitrogen