title: Immunoproteomics-Based Analysis of the Immunocompetent Serological Response to Lomentospora prolificans.
abstract: The filamentous fungus Lomentospora prolificans is an emerging pathogen causing severe infections mainly among the immunocompromised population. These diseases course with high mortality rates due to great virulence of the fungus, its inherent resistance to available antifungals, and absence of specific diagnostic tools. Despite being widespread in humanized environments, L. prolificans rarely causes infections in immunocompetent individuals likely due to their developed protective immune response. In this study, conidial and hyphal immunomes against healthy human serum IgG were analyzed, identifying immunodominant antigens and establishing their prevalence among the immunocompetent population. Thirteen protein spots from each morph were detected as reactive against at least 70% of serum samples, and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Hence, the most seroprevalent antigens were WD40 repeat 2 protein, malate dehydrogenase, and DHN1, in conidia, and heat shock protein (Hsp) 70, Hsp90, ATP synthase β subunit, and glyceraldehyde-3-phosphate dehydrogenase, in hyphae. More interestingly, the presence of some of these seroprevalent antigens was determined on the cell surface, as Hsp70, enolase, or Hsp90. Thus, we have identified a diverse set of antigenic proteins, both in the entire proteome and cell surface subproteome, which may be used as targets to develop innovative therapeutic or diagnostic tools.
authors.affiliation: Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.
authors.forename: Aize
authors.initials: A
authors.lastname: Pellon
authors.affiliation: Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.
authors.forename: Andoni
authors.initials: A
authors.lastname: Ramirez-Garcia
authors.affiliation: Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.
authors.forename: Idoia
authors.initials: I
authors.lastname: Buldain
authors.affiliation: Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.
authors.forename: Aitziber
authors.initials: A
authors.lastname: Antoran
authors.affiliation: Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.
authors.forename: Aitor
authors.initials: A
authors.lastname: Rementeria
authors.affiliation: Fungal and Bacterial Biomics Research Group. Department of Immunology, Microbiology and Parasitology. Faculty of Science and Technology. University of the Basque Country (UPV/EHU) , Leioa 48940, Spain.
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Hernando
mesh_terms: D000946:Antigens, Fungal / Q000276:immunology / Q000378:metabolism; D001203:Ascomycota / Q000276:immunology* / Q000502:physiology; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D019084:Fluorescent Antibody Technique, Indirect; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D025301:Hyphae / Q000276:immunology / Q000378:metabolism; D015151:Immunoblotting; D007121:Immunocompetence / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D009181:Mycoses / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D020543:Proteome / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013172:Spores, Fungal / Q000276:immunology / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000946:Antigens, Fungal; D005656:Fungal Proteins; D007074:Immunoglobulin G; D020543:Proteome
keywords: Lomentospora prolificans; Scedosporium; immunome; proteome; serum IgG; surfaceome; two-dimensional electrophoresis (2-DE)