title: Effect of Ionic and Nonionic Carriers in Electrical Field-Flow Fractionation.
abstract: A major limitation of electrical field-flow fractionation (ElFFF) is the polarization of the electrodes that occurs when using an ionic carrier liquid. As there is great interest in using ElFFF with biological materials and biological materials typically have high ionic strengths and high osmotic concentrations, we explore the effect of concentration for phosphate buffered saline (PBS), a typical ionic medium for biological samples, and for two nonionic materials common in bioparticle analysis: isopropanol (IPA) and sucrose. Their effect on retention and separations in ElFFF for increasing concentrations was observed. The results suggest that modifying the carrier solution with PBS, sucrose, and/or IPA would enable characterization and separation of biological samples in ElFFF. Specifically, changes of elution time and electrical parameters such as current, conductivity, and bulk channel resistance were observed as functions of carrier ionic and osmotic strength for the different carrier additives. PBS can be used in the micromolar range, equivalent to about 0.1% 1× PBS (150 μM). These concentrations are far from the isotonic condition of PBS (∼ 150 mM) that is normally used with biological samples. However, the nonionic additive carriers IPA and sucrose show quality retention even when added in high concentrations. The results show that IPA could be used in ratios up to 60% and that sucrose can be used in concentrations up to 0.3 M. Concentrations of 2% IPA (0.26 M) and 0.30 M sucrose are biologically isotonic conditions (275-299 mOsm/kg), and retention was readily obtained in these conditions using both DC ElFFF and cyclical ElFFF (CyE1FFF). Carriers of this type may make it possible to use ElFFF with biological samples.
authors.affiliation: Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University , Rama VI Rd., Bangkok 10400, Thailand.|Branches of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University , Bangna-Trad Rd., Samut Prakarn 10540, Thailand.
authors.forename: Mathuros
authors.initials: M
authors.lastname: Ornthai
authors.affiliation: Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University , Rama VI Rd., Bangkok 10400, Thailand.
authors.forename: Atitaya
authors.initials: A
authors.lastname: Siripinyanond
authors.affiliation: Department of Mechanical Engineering, University of Utah , 1495 E 100 S, Room 1550 MEK, Salt Lake City, Utah 84112, United States.
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Gale
mesh_terms: D019840:2-Propanol / Q000737:chemistry*; D004560:Electricity; D004566:Electrodes; D031521:Fractionation, Field Flow; D007477:Ions / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry*
chemical_list: D007477:Ions; D010710:Phosphates; D012965:Sodium Chloride; D013395:Sucrose; D019840:2-Propanol