title: The Anti-Adipogenic Potential of COUP-TFII Is Mediated by Downregulation of the Notch Target Gene Hey1.
abstract: BACKGROUND
  Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) belongs to the steroid/thyroid hormone receptor superfamily and may contribute to the pathogenesis of obesity. It has not conclusively been established, however, whether its role is pro- or anti-adipogenic.
  
  
  RESULTS
  Gene silencing of Coup-tfII in 3T3-F442A preadipocytes resulted in enhanced differentiation into mature adipocytes. This was associated with upregulation of the Notch signaling target gene Hey1. A functional role of Hey1 was confirmed by gene silencing in 3T3-F442A preadipocytes, resulting in impaired differentiation. In vivo, de novo fat pad formation in NUDE mice was significantly stimulated following injection of preadipocytes with Coup-tfII gene silencing, but impaired with Hey1 gene silencing. Moreover, expression of Coup-tfII was lower and that of Hey1 higher in isolated adipocytes of obese as compared to lean adipose tissue.
  
  
  CONCLUSIONS
  These in vitro and in vivo data support an anti-adipogenic role of COUP-TFII via downregulating the Notch signaling target gene Hey1.
authors.affiliation: KU Leuven, University of Leuven, Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, B-3000, Leuven, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Scroyen
authors.affiliation: KU Leuven, University of Leuven, Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, B-3000, Leuven, Belgium.
authors.forename: Dries
authors.initials: D
authors.lastname: Bauters
authors.affiliation: KU Leuven, University of Leuven, Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, B-3000, Leuven, Belgium.
authors.forename: Christine
authors.initials: C
authors.lastname: Vranckx
authors.affiliation: KU Leuven, University of Leuven, Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, B-3000, Leuven, Belgium.
authors.forename: H Roger
authors.initials: HR
authors.lastname: Lijnen
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D050154:Adiposity / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D051837:COUP Transcription Factor II / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D017576:Daptomycin / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D020868:Gene Silencing / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D051880:Receptors, Notch / Q000378:metabolism*
chemical_list: D051837:COUP Transcription Factor II; D018797:Cell Cycle Proteins; C120420:Hey1 protein, mouse; D051880:Receptors, Notch; D017576:Daptomycin