title: Analysis of Assembly and Budding of Lujo Virus.
abstract: The recently identified arenavirus Lujo virus (LUJV) causes fatal hemorrhagic fever in humans. We analyzed its mechanism of viral release driven by matrix protein Z and the cell surface glycoprotein precursor GPC. The L domains in Z are required for efficient virus-like particle release, but Tsg101, ALIX/AIP1, and Vps4A/B are unnecessary for budding. LUJV GPC is cleaved by site 1 protease (S1P) at the RKLM motif, and treatment with the S1P inhibitor PF-429242 reduced LUJV production.
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Shuzo
authors.initials: S
authors.lastname: Urata
authors.affiliation: Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Weyer
authors.affiliation: Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa.
authors.forename: Nadia
authors.initials: N
authors.lastname: Storm
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan The Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Miyazaki
authors.affiliation: Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa.
authors.forename: Petrus Jansen
authors.initials: PJ
authors.lastname: van Vuren
authors.affiliation: Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa.
authors.forename: Janusz Tadeusz
authors.initials: JT
authors.lastname: Paweska
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan The Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan j-yasuda@nagasaki-u.ac.jp.
authors.forename: Jiro
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D063927:Lujo virus / Q000254:growth & development / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism; D019065:Virus Assembly; D057074:Virus Release
chemical_list: D014764:Viral Proteins