title: HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID mice.
abstract: Human adenovirus (HAdV) vectors are promising tools for cancer therapy, but the shortage of efficient animal models for productive HAdV infections has restricted the evaluation of systemic effects to mainly immunodeficient mice. Previously, we reported a highly efficient replication of HAdV-2 in a non-tumorigenic mouse mammary epithelial cell line, NMuMG. Here we show that HAdV-2 gene expression and progeny formation in NMuMG cells transformed with the SV40 T antigen (NMuMG-T cells) were as efficient as in the parental NMuMG cells. Injection of HAdV-2 into tumours established by NMuMG-T in SCID mice caused reduced tumour growth and signs of intratumoural lesions. HAdV-2 replicated within the NMuMG-T-established tumours, but not in interspersed host-derived tissues within the tumours. The specific infection of NMuMG-T-derived tumours was verified by the lack of viral DNA in kidney, lung or spleen although low levels of viral DNA was occasionally found in liver.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Immunology, Genetics and Pathology, Uppsala University, Sweden.
authors.forename: Di
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden.
authors.forename: Elisabeth J M
authors.initials: EJ
authors.lastname: Huijbers
authors.affiliation: Department of Immunology, Genetics and Pathology, Uppsala University, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Essand
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Akusjärvi
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden.
authors.forename: Staffan
authors.initials: S
authors.lastname: Johansson
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden. Electronic address: cath@imbim.uu.se.
authors.forename: Catharina
authors.initials: C
authors.lastname: Svensson
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000821:virology*; D005260:Female; D006801:Humans; D008321:Mammary Glands, Animal / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D009369:Neoplasms / Q000503:physiopathology / Q000821:virology*
chemical_list: D000952:Antigens, Polyomavirus Transforming
keywords: Anti-tumour activity; Murine cells; Oncolytic adenovirus; Permissive infection; Viral replication