title: Protective effect of bone marrow mesenchymal stem cells on PC12 cells apoptosis mediated by TAG1.
abstract: OBJECTIVE
  This study aims to explore the protection effect of bone marrow mesenchymal stem cells (BMSCs) on PC12 cells apoptosis mediated by transient axonal glycoprotein 1 (TAG1).
  
  
  METHODS
  PC12 cells were divided into control group, Aβ25-35 group and BMSCs + Aβ25-35 group. The effects of BMSCs on PC12 cells treated by Aβ25-35 were detected using MTT, Hoechst 33258 and Annexin V-FITC/PI staining methods. The expression levels of TAG1, β-amyloid precursor protein (APP), AICD and p53 were determined by RT-PCR and Western blotting methods. The expression levels of Bax and Bcl-2 were determined by Western blotting method. The activity of Caspase 3 was detected by spectrophotometric method.
  
  
  RESULTS
  MTT results showed that cell activity decreased after the treatment of 20 μM Aβ25-35 for 48 h (P<0.01) while it increased in BMSCs + Aβ25-35 group (P<0.01). Hoechst 33258 and Annexin V-FITC/PI staining results showed that Aβ25-35 could induce the apoptosis of PC12 cells while the apoptosis of PC12 cells was inhibited in BMSCs + Aβ25-35 group. RT-PCR and Western blotting methods showed that 20 μM Aβ25-35 could increase the expression levels of TAG1, APP, AICD and p53 (P<0.01) while they decreased in BMSCs + Aβ25-35 group (P<0.01). 20 μM Aβ25-35 could increase the expression levels of Bax and decrease the expression levels of Bcl-2 (P<0.01), while the expression levels of Bax decreased and the expression levels of Bcl-2 increase in BMSCs + Aβ25-35 group (P<0.01). 20 μM Aβ25-35 could enhance Caspase 3 activity while it decreased in BMSCs + Aβ25-35 group (P<0.01). Conclusions BMSCs with Aβ25-35 could inhibit the apoptosis of PC12 cells, which maybe related with TAG1/APP/AICD signal pathway.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, P. R. China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, P. R. China.
authors.forename: Ji-Yu
authors.initials: JY
authors.lastname: Lou
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, P. R. China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Bai
authors.affiliation: Department of Neurology, 148 Hospital of PLA Zibo, P. R. China.
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Neurology, 148 Hospital of PLA Zibo, P. R. China.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Li
authors.affiliation: Department of Neurology, 148 Hospital of PLA Zibo, P. R. China.
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Yin
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism; D018920:Coculture Techniques; D058969:Contactin 2 / Q000378:metabolism*; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; D058969:Contactin 2
keywords: BMSCs; PC12 cells; TAG1; apoptosis