title: Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis.
abstract: BACKGROUND
  ImmunoCAP ISAC 112, is a commercially available molecular allergy IgE multiplex test. Data on the comparison of this rather novel test with extract-based as well as molecular ImmunoCAP singleplex IgE tests is missing.
  
  
  OBJECTIVE
  To perform a comparison between the ISAC multiplex IgE assay and the ImmunoCAP singleplex test results.
  
  
  METHODS
  Serum samples of 101 adults with grass pollen allergy were analysed for sIgE to 112 allergenic molecules represented on the ISAC test as well as to common atopy-related extract-based allergy tests with the ImmunoCAP System (house dust mite [d1], cat [e1], dog [e5], cow's milk [f2], hen's egg [f1], hazelnut [f17], celery [f85],Alternaria alternate[m6], as well as pollen from birch [t3], hazel [t4], mugwort [w6], and ragweed [w1]). Subsequently statistical analysis was performed with the Spearman rank correlation test and the Clopper-Pearson method in order to compare the ISAC multiplex results with the sIgE singleplex results.
  
  
  RESULTS
  The positive percent agreements (PPA) and negative percent agreement (NPA) of corresponding allergens between the ISAC sIgE test and the extract-based singleplex ImmunoCAP results at cutoff 0.1 kUA/l varied between 60-100 % for PPA and 78-97 % for NPA.
  
  
  CONCLUSIONS
  When taking into account corresponding allergens molecular testing with the ISAC multiplex test correlates well with ImmunoCAP singleplex results.
authors.affiliation: Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany ; Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany ; AbbVie Deutschland GmbH & Co. KG, Mainzer Straße 81, 65189 Wiesbaden, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Huss-Marp
authors.affiliation: Department of Dermatology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Gutermuth
authors.affiliation: Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Schäffner
authors.affiliation: Department of Dermatology and Allergy, Technische Universität München, Munich, Germany ; ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Darsow
authors.affiliation: Department of Dermatology and Allergy, Technische Universität München, Munich, Germany ; ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Pfab
authors.affiliation: Department of Dermatology and Allergy, Technische Universität München, Munich, Germany ; ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Brockow
authors.affiliation: Department of Dermatology and Allergy, Technische Universität München, Munich, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Ring
authors.affiliation: ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
authors.forename: Heidrun
authors.initials: H
authors.lastname: Behrendt
authors.affiliation: Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany.
authors.forename: Thilo
authors.initials: T
authors.lastname: Jakob
authors.affiliation: University Freiburg Medical Center, Department of Exercise Medicine and Sport, University of Freiburg, Freiburg, Germany ; Department of Pneumology, University of Freiburg, Freiburg, Germany ; Department of Pneumology, Freiburg University Medical Center, Albert-Ludwigs-University, Freiburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Ahlgrim
keywords: ISAC; Immuno-CAP; Molecular allergy diagnostics; allergens; component resolved diagnosis; extract-based diagnostics; singleplex multiplex; specific IgE