title: Spatial and temporal expression of histone demethylase, Kdm2a, during murine molar development.
abstract: The histone demethylase, lysine (K)-specific demethylase 2A (Kdm2a), is highly conserved and expressed ubiquitously. Kdm2a can regulate cell proliferation and osteo/dentinogenic, adipogenic and chondrogenic differentiation of mesenchymal stem cells (MSCs) derived from dental tissue. We used quantitative real-time RT-PCR analysis and immunohistochemistry to detect Kdm2a expression during development of the murine molar at embryonic days E12, E14, E16 and E17 and postnatal days P3 and P14. Immunohistochemistry results showed no positive staining of Kdm2a at E12. At E14, Kdm2a was expressed weakly in the inner enamel epithelium, stellate reticulum cells and dental sac. At E16, Kdm2a was expressed mainly in the inner and outer enamel epithelium, stratum intermedium and dental sac, but weaker staining was found in cervical loop and dental papilla cells adjacent to the basement membrane. At E17, the strongest Kdm2a staining was detected in the ameloblasts and stronger Kdm2a staining also was detected in the stratum intermedium, outer enamel epithelium and dental papilla cells compared to the expression at E16. Postnatally, we found that Kdm2a was localized in secretory and mature ameloblasts and odontoblasts, and dentin was unstained. Real-time RT-PCR showed that Kdm2a mRNA levels in murine germ cells increased from E12 to E14 and from E14 to E16; no significant change occurred at E16, E17 or P3, then the levels decreased at P14 compared to P3. Kdm2a expression may be closely related to cell proliferation, to ameloblast and odontoblast differentiation and to the secretion of extracellular enamel and dentin during murine tooth development.
authors.affiliation: a Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology , Beijing , China.|d Xiangya Stomatology Hospital, Central South University , Changsha, Hunan , China.|e School of Stomatology, Central South University , Changsha, Hunan , China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yi
authors.affiliation: a Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology , Beijing , China.|f Department of General Dentistry , Capital Medical University School of Stomatology , Beijing , China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.affiliation: d Xiangya Stomatology Hospital, Central South University , Changsha, Hunan , China.|e School of Stomatology, Central South University , Changsha, Hunan , China.
authors.forename: O S
authors.initials: OS
authors.lastname: Liu
authors.affiliation: d Xiangya Stomatology Hospital, Central South University , Changsha, Hunan , China.|e School of Stomatology, Central South University , Changsha, Hunan , China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Lu
authors.affiliation: b Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology , Beijing , China.|c Department of Biochemistry and Molecular Biology , Capital Medical University School of Basic Medical Sciences , Beijing , China.
authors.forename: J S
authors.initials: JS
authors.lastname: Wang
authors.affiliation: b Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology , Beijing , China.|c Department of Biochemistry and Molecular Biology , Capital Medical University School of Basic Medical Sciences , Beijing , China.
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.affiliation: g Department of Pediatrics , Stomatological Hospital of Nankai University , Tianjin , China.
authors.forename: R
authors.initials: R
authors.lastname: Yao
authors.affiliation: a Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology , Beijing , China.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Fan
mesh_terms: D000565:Ameloblasts / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009805:Odontogenesis / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D014083:Tooth Germ / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C547579:FBXL11 protein, mouse; D056484:Jumonji Domain-Containing Histone Demethylases
keywords: Kdm2a; development; expression patterns; histone demethylase; mouse; tooth