title: Monitoring time course of human whole blood coagulation using a microfluidic dielectric sensor with a 3D capacitive structure.
abstract: This paper reports on the design, fabrication, and testing of a microfluidic sensor for dielectric spectroscopy (DS) of human whole blood during coagulation. The sensor employs a three-dimensional (3D), parallel-plate, capacitive sensing structure with a floating electrode integrated into a microfluidic channel. Using an impedance analyzer and after a 5-point calibration, the sensor is shown to measure the real part of complex relative dielectric permittivity of human whole blood in a frequency range of 10kHz to 100MHz. The temporal variation of dielectric permittivity at 1MHz for human whole blood from three different healthy donors shows a peak in permittivity at ~ 4 to 5 minutes, which also corresponds to the onset of CaCl2-initiated coagulation of the blood sample verified visually.
authors.forename: Debnath
authors.initials: D
authors.lastname: Maji
authors.forename: Michael A
authors.initials: MA
authors.lastname: Suster
authors.forename: Evi
authors.initials: E
authors.lastname: Stavrou
authors.forename: Umut A
authors.initials: UA
authors.lastname: Gurkan
authors.forename: Pedram
authors.initials: P
authors.lastname: Mohseni
mesh_terms: D001777:Blood Coagulation / Q000502:physiology*; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*