title: Apigenin inhibits TGF-β1-induced proliferation and migration of airway smooth muscle cells.
abstract: It is well known that the proliferation and migration of ASM cells (ASMCs) plays an important role in the pathogenesis of airway remodeling in asthma. Previous studies reported that apigenin can inhibit airway remodeling in a mouse asthma model. However, its effects on the proliferation and migration of ASMCs in asthma remain unknown. Therefore, the aim of our present study was to investigate the effects of apigenin on ASMC proliferation and migration, and explore the possible molecular mechanism. We found that apigenin inhibited transforming growth factor-β1 (TGF-β1)-induced ASMC proliferation. The cell cycle was blocked at G1/S-interphase by apigenin. It also suppressed TGF-β1-induced ASMCs migration. Furthermore, apigenin inhibited TGF-β1-induced Smad 2 and Smad 3 phosphorylation in ASMCs. Taken together, these results suggested that apigenin inhibited the proliferation and migration of TGF-β1-stimulated ASMCs by inhibiting Smad signaling pathway. These data might provide useful information for treating asthma and show that apigenin has potential for attenuating airway remodeling.
authors.affiliation: Department of Respiratory Medicine, People's Hospital of Zhengzhou Zhengzhou 450003, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, People's Hospital of Zhengzhou Zhengzhou 450003, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Respiratory Medicine, People's Hospital of Zhengzhou Zhengzhou 450003, China.
authors.forename: Hong-Ke
authors.initials: HK
authors.lastname: Wu
authors.affiliation: Department of Respiratory Medicine, People's Hospital of Zhengzhou Zhengzhou 450003, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, People's Hospital of Zhengzhou Zhengzhou 450003, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Yao
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects*; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001249:Asthma / Q000503:physiopathology*; D015153:Blotting, Western; D001980:Bronchi / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D018927:Anti-Asthmatic Agents; D053773:Transforming Growth Factor beta1; D047310:Apigenin
keywords: Asthma; airway smooth muscle cells; apigenin; transforming growth factor-β1