title: Development of a 3-hydroxypropionate resistant Escherichia coli strain.
abstract: 3-hydroxypropionate (3HP) is an important platform chemical, and its biosynthesis is severely restricted by the toxicity of 3HP on cell growth and survival. To improve Escherichia coli resistance to 3HP and reduce the total production cost in industrial applications, we have identified variations in protein expression level exposed to sub-lethal concentration of this chemical using 2-dimensional gel electrophoresis. Under 3HP stress, the amount of 46 proteins was increased while the amount of 23 proteins was reduced. According to the proteomic results, overexpression of some identified proteins significantly increased the E. coli survival rate under 3HP stress. This study shed light on clues for developing E. coli strains with higher resistance to 3HP toxicity and lower production cost for industrial applications.
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.|b University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.|b University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Han
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.affiliation: c Institute of Oceanology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Ding
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D060847:Metabolic Engineering / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects; D011422:Propionates / Q000378:metabolism* / Q000633:toxicity; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D029968:Escherichia coli Proteins; D011422:Propionates; D011994:Recombinant Proteins
keywords: 3-hydroxypropionate; 3-hydroxypropionate resistance; Escherichia coli; bacterial stress response; comparative proteomics