title: Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells.
abstract: Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.
authors.affiliation: Baker IDI Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.
authors.forename: Nigora
authors.initials: N
authors.lastname: Mukhamedova
authors.affiliation: Department of Microbiology, Immunology and Tropical Medicine, George Washington University School of Medicine and Health Sciences, Ross Hall, Room 624, 2300 Eye Street, N.W., Washington, DC, 20037, USA.
authors.forename: Beda
authors.initials: B
authors.lastname: Brichacek
authors.affiliation: Department of Microbiology, Immunology and Tropical Medicine, George Washington University School of Medicine and Health Sciences, Ross Hall, Room 624, 2300 Eye Street, N.W., Washington, DC, 20037, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Darwish
authors.affiliation: Office of VP for Research, George Washington University Center for Microscopy and Image Analysis, Washington, DC, 20037, USA.
authors.forename: Anastas
authors.initials: A
authors.lastname: Popratiloff
authors.affiliation: Baker IDI Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Sviridov
authors.affiliation: Department of Microbiology, Immunology and Tropical Medicine, George Washington University School of Medicine and Health Sciences, Ross Hall, Room 624, 2300 Eye Street, N.W., Washington, DC, 20037, USA. mbukrins@gwu.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Bukrinsky
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D015658:HIV Infections / Q000378:metabolism*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods*; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D054311:nef Gene Products, Human Immunodeficiency Virus; D002784:Cholesterol
keywords: ABCA1; Cholesterol efflux; Confocal microscopy; HIV-1; Image analysis; Nef