title: Consumer acceptability of liquorice root (Glycyrrhiza glabra L.) as an alternative sweetener and correlation with its bioactive content and biological activity.
abstract: Consumer acceptability and sensory properties of liquorice root (Glycyrrhiza glabra L.) were evaluated. Quantitation of total polyphenolics and glycyrrhizic acid (GA), as well as the antioxidant capacity of liquorice extracts, was conducted and their biological effects (cytotoxic, antioxidative/pro-oxidative activity, lipid peroxidation on human laryngeal carcinoma cell line) compared to the ones of their predominant bioactive compound - GA. Conducted consumer survey revealed poor familiarity with liquorice (12.37% of correspondents), but willingness towards its use as an alternative sweetener (77.32% of consumers). Polyphenolic content of evaluated extracts ranged from 1018.18 to 1277.27 mg gallic acid equivalents (GAE)/l while GA content varied between 2179.53 and 2944.13 mg/l. The most pronounced cytotoxic effect (60%) and lipid peroxidation were exerted by treatment with the highest applied extract concentrations (10 mg/ml). Pure GA exhibited cytotoxic and pro-oxidative effects at concentrations of 0.12-0.6 mg/ml. Due to high GA content, coupled with its pronounced cytotoxic activity, the intake of liquorice root should be limited.
authors.affiliation: a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
authors.forename: Draženka
authors.initials: D
authors.lastname: Komes
authors.affiliation: a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Belščak-Cvitanović
authors.affiliation: a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
authors.forename: Slaven
authors.initials: S
authors.lastname: Jurić
authors.affiliation: a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
authors.forename: Arijana
authors.initials: A
authors.lastname: Bušić
authors.affiliation: a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Vojvodić
authors.affiliation: a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
authors.forename: Ksenija
authors.initials: K
authors.lastname: Durgo
mesh_terms: D000293:Adolescent; D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D003258:Consumer Behavior; D003603:Cytotoxins / Q000032:analysis / Q000494:pharmacology*; D005260:Female; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000494:pharmacology; D013549:Sweetening Agents / Q000032:analysis / Q000494:pharmacology*; D013649:Taste; D055815:Young Adult
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D003603:Cytotoxins; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013549:Sweetening Agents; D019695:Glycyrrhizic Acid
keywords: Antioxidative/pro-oxidative activity; cytotoxicity; lipid peroxidation; liquorice; sensory properties