title: Preparation, characterization and in vivo investigation of blood-compatible hemoglobin-loaded nanoparticles as oxygen carriers.
abstract: Although many attempts have been made to design advanced hemoglobin-based oxygen carriers (HBOCs), no clinically viable product has been widely approved, because they do not perform normal blood functions, such as coagulation, hematologic reactions and stability. Additionally, the in vivo oxygenation of hemoglobin-loaded nanoparticles (HbPs) encapsulated with polymers has seldom been proved. Herein, HbPs of approximately 200nm with good stability were successfully fabricated and exhibited oxygen-carrying capacity. The HbPs preserve the biological and structure features of hemoglobin according to UV-vis spectrophotometry, Fourier transform infrared (FTIR) spectroscopy and circular dichroism (CD) spectral analysis. In vitro, the HbPs showed a viscosity comparable to that of blood with no obvious effects on red blood cell aggregation. At the same time, blood compatibility was characterized in terms of platelet function, clot strength, speed of clot formation, degree of fibrin cross-linking and hemolysis rate. After intravenous administration of HbPs to mice with controlled hemorrhages, blood flow recovery and maintenance of systemic oxygenation were observed.
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Lu
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Caiyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: You
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Hongzhang
authors.initials: H
authors.lastname: Deng
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Gan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xia
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Xianlei
authors.initials: X
authors.lastname: Li
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Leihou
authors.initials: L
authors.lastname: Shao
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China. Electronic address: wuy@nanoctr.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: zhaolian@bmi.ac.cn.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: zhouhtt1966@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D001802:Blood Substitutes / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002417:Cattle; D004903:Erythrocyte Aggregation / Q000187:drug effects; D005337:Fibrin / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006461:Hemolysis; D006470:Hemorrhage / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010316:Particle Size; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014783:Viscosity; D014914:Whole Blood Coagulation Time
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D005337:Fibrin; D010100:Oxygen
keywords: Blood compatibility; Hemoglobin; In vivo oxygenation; Nanoparticles; Oxygen carrier