title: Radiation-synthesized protein-based drug carriers: Size-controlled BSA nanoparticles.
abstract: Nanotechnology has broadened the options for the delivery of agents of biotechnological and clinical relevance. Currently, attention has been driven towards the development of protein-based nanocarriers due to high the biocompatibility and site-specific delivery. In this work we report radiation-synthesized bovine serum albumin nanoparticles as an attempt to overcome limitations of available albumin particles, as a novel route for the development of crosslinked protein nanocarriers for the administration of chemotherapic agents or radiopharmaceuticals. Albumin containing phosphate buffer solutions were irradiated using γ-irradiation at distinct cosolvent concentrations-ethanol or methanol. Nanoparticle size was followed by DLS and bityrosine crosslinking formation using fluorescence measurements and SDS-PAGE. In addition, computational experiments were performed to elucidate the mechanism and pathways for the nanoparticle formation. The synthesis of BSA nanoparticles using γ-irradiation in the presence of a cosolvent allowed the formation of the nanoparticles from 7 to 70 nm without the use of any chemical crosslinker as confirmed by SDS-PAGE and DLS analysis. The combination of cosolvent and γ-irradiation allowed a fine tuning with regard to protein size.
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), Av. Prof. Lineu Prestes, 2242, Cidade Universitária, 05508-000 São Paulo, SP, Brazil. Electronic address: rodgqueiroz@gmail.com.
authors.forename: R G
authors.initials: RG
authors.lastname: Queiroz
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), Av. Prof. Lineu Prestes, 2242, Cidade Universitária, 05508-000 São Paulo, SP, Brazil. Electronic address: varca@usp.br.
authors.forename: G H C
authors.initials: GH
authors.lastname: Varca
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590, Lodz, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Kadlubowski
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590, Lodz, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Ulanski
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), Av. Prof. Lineu Prestes, 2242, Cidade Universitária, 05508-000 São Paulo, SP, Brazil.
authors.forename: A B
authors.initials: AB
authors.lastname: Lugão
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D005720:Gamma Rays; D008958:Models, Molecular; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D011827:Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012997:Solvents; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D004337:Drug Carriers; D011506:Proteins; D012997:Solvents; D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; Gamma-irradiation; Nanocarrier; Protein crosslinking; Protein-based nanoparticles