title: Assessment of a Flavone-Polysaccharide Based Prescription for Treating Duck Virus Hepatitis.
abstract: Because polysaccharide and flavone ingredients display good antiviral activity, we developed a flavone/polysaccharide-containing prescription that would be effective against duck viral hepatitis (DVH) and investigated its hepatoprotective effects. Flavones were derived from Hypericum japonicum (HJF) (entire herb of Hypericum japonicum Thunb) and Salvia plebeia (SPF) (entire herb of Salvia plebeia R. Br.), and polysaccharides were derived from Radix Rehmanniae Recens (RRRP) (dried root of Rehmannia glutinosa Libosch). This prescription combination was based on the theory of syndrome differentiation and treatment in traditional Chinese veterinary medicine. In vitro and in vivo experiments were conducted using the three single ingredients compared to the combined HRS prescription to determine their anti-duck hepatitis A viral (anti-DHAV) activity. The results showed that all experimental conditions displayed anti-DHAV activity, but the HRS prescription presented the best effect. To further investigate the hepatoprotective effect of the HRS prescription on DHAV-induced hepatic injury, we tested the mortality rate, the hepatic pathological severity score, plasma biochemical indexes of hepatic function, blood DHAV gene expression levels and peroxidation damage evaluation indexes and then analyzed correlations among these indexes. The results demonstrated that the HRS prescription significantly decreased the mortality rate, reduced the severity of hepatic injury, decreased the hepatic pathological severity score, depressed blood DHAV gene expression levels, and returned the indexes of hepatic function and peroxidation almost to a normal level. These results indicate that the HRS prescription confers an outstanding hepatoprotective effect, and we expect that it will be developed into a new candidate anti-DHAV drug.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Du
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Shuaibing
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Meiyun
authors.initials: M
authors.lastname: Song
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Fangke
authors.initials: F
authors.lastname: Yao
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000627:therapeutic use*; D004372:Ducks / Q000821:virology*; D047309:Flavones / Q000737:chemistry / Q000627:therapeutic use*; D004371:Hepatitis Virus, Duck / Q000187:drug effects*; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy* / Q000473:pathology; D020902:Hypericum / Q000737:chemistry; D000068378:Lamiales / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000821:virology; D010850:Picornaviridae Infections / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use*; D027544:Salvia / Q000737:chemistry; D019155:Veterinary Drugs / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000998:Antiviral Agents; D047309:Flavones; D011134:Polysaccharides; D019155:Veterinary Drugs