title: Structural Studies of Component of Lysoamidase Bacteriolytic Complex from Lysobacter sp. XL1.
abstract: The lysoamidase bacteriolytic complex (LBC) comprising five enzymes (L1-L5) is secreted into the culture liquid by gram-negative bacterium Lysobacter sp. XL1. The medicinal agent lysoamidase has a broad-antimicrobial spectrum. Bacteriolytic protease L1 belongs to the LBC. Recombinant L1 protease of Lysobacter sp. XL1 was expressed, purified to homogeneity and crystallized. The X-ray structure of L1 at 1.35 Å resolution has been determined using the synchrotron data and the molecular replacement method. L1 protease is a thermostable whose thermal unfolding proceeds in one step without forming stable intermediates. Structural information concerning L1 will contribute to the development of new-generation antimicrobial drugs, whose application will not be accompanied by the selection of resistant microorganisms.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Institutskaya str. 4, Pushchino, 142290, Moscow Region, Russian Federation. sveta@vega.protres.ru.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tishchenko
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Institutskaya str. 4, Pushchino, 142290, Moscow Region, Russian Federation.
authors.forename: Azat
authors.initials: A
authors.lastname: Gabdulkhakov
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Institutskaya str. 4, Pushchino, 142290, Moscow Region, Russian Federation.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Melnik
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Moscow Region, Russian Federation.
authors.forename: Irina
authors.initials: I
authors.lastname: Kudryakova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Moscow Region, Russian Federation.
authors.forename: Oleg
authors.initials: O
authors.lastname: Latypov
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Moscow Region, Russian Federation.
authors.forename: Natalya
authors.initials: N
authors.lastname: Vasilyeva
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Moscow Region, Russian Federation.
authors.forename: Alexey
authors.initials: A
authors.lastname: Leontievsky
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D053528:Lysobacter / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D058767:Protein Unfolding
chemical_list: C060332:lysoamidase; D010447:Peptide Hydrolases
keywords: Bacteriolytic protease L1; Circular dichroism; Crystals; Lysobacter sp. XL1; Structure; Thermal unfolding