title: Epigenetic Silencing of the Putative Tumor Suppressor Gene GLDC (Glycine Dehydrogenase) in Gastric Carcinoma.
abstract: BACKGROUND
  The metabolic enzyme, glycine dehydrogenase (GLDC), involved in glycine metabolism, is known to be involved in non-ketotic hyperglycinemia but not in cancer. Herein, we investigated GLDC expression and its promoter methylation in gastric cancer (GC).
  
  
  METHODS
  GLDC expression and epigenetics were investigated using GC cell lines and tissues. Functional studies were also performed for identification of a correlation between methylated GLDC genes and gastric cancer progression.
  
  
  RESULTS
  The results of the study can be summarized as follows: (i) GLDC was silenced in GC cell lines and tissues. The down-regulation of GLDC was closely linked to promoter methylation. (ii) Knockdown of GLDC increased cell proliferation, migration, invasion, colony formation and reduced apoptosis. (iii) In GC tissues, hypermethylation of GLDC had a significant correlation with down-regulation of the GLDC protein compared to normal gastric tissues.
  
  
  CONCLUSIONS
  GLDC is a putative tumor suppressor gene involved in gastric cancer progression and hypermethylation of the GLDC promoter regulates its transcriptional silencing.
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea Department of Cancer Biology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyae Lim
authors.initials: HL
authors.lastname: Min
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea Department of Cancer Biology, Seoul National University College of Medicine, Seoul, Republic of Korea Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Woo Ho
authors.initials: WH
authors.lastname: Kim
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Bo Gun
authors.initials: BG
authors.lastname: Jang
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea everest@snu.ac.kr.
authors.forename: Min A
authors.initials: MA
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000379:methods*; D005260:Female; D020868:Gene Silencing / Q000502:physiology*; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D050789:Glycine Dehydrogenase / Q000235:genetics*; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D050789:Glycine Dehydrogenase
keywords: DNA methylation; Glycine dehydrogenase; stomach neoplasms; tumor suppressor gene