title: Monitoring the microbial community shift throughout the shock changes of hydraulic retention time in an anaerobic moving bed membrane bioreactor.
abstract: An anaerobic moving bed membrane bioreactor (AnMBMBR) fed with synthetic domestic wastewater was investigated under hydraulic retention time (HRT) shocks to assess the effects on the microbial (bacteria and archaea) community and reactor performance. 16S rDNA targeted polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach was optimized to relate the metabolic and community composition with biogas generation, methane content and COD removal efficiency. From the drastic decrease of HRT (from 8 h to 4 h), the methane production was significantly reduced due to the HRT shock, while the COD removal efficiency was not affected. The enhanced growth of homoacetogenic bacteria, Thermoanaerobacteraceae competes with methanogens under shock period. When the HRT was recovered to 8 h, the methane generation rate was higher than the initial operation before the shock HRT changes, which would be ascribed to the activity of new emerging hydrogenotrophic archaea, Methanocella sp. and Methanofollis sp.
authors.affiliation: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Biotechnology Research Department, Ministry of Science and Technology, Myanmar.
authors.forename: Theint Theint
authors.initials: TT
authors.lastname: Win
authors.affiliation: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Kangwoo
authors.initials: K
authors.lastname: Cho
authors.affiliation: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. Electronic address: kgsong@kist.re.kr.
authors.forename: Kyung Guen
authors.initials: KG
authors.lastname: Song
authors.affiliation: Department of Civil and Environmental Engineering, Yonsei University, Republic of Korea.
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D016172:DNA Fingerprinting; D058645:Denaturing Gradient Gel Electrophoresis; D008567:Membranes, Artificial; D008697:Methane / Q000378:metabolism; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D008697:Methane
keywords: AnMBMBR; Biogas; HRT shock; Microbial community; PCR–DGGE