title: Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens.
abstract: We evaluated a multiplexed PCR panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. Panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. Overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively.
authors.affiliation: University of Utah Department of Medicine, Division of Infectious Diseases, School of Medicine, Salt Lake City, Utah, USA University of Utah Department of Pathology, Clinical Microbiology, Salt Lake City, Utah, USA kim.hanson@hsc.utah.edu anne.blaschke@hsc.utah.edu.
authors.forename: K E
authors.initials: KE
authors.lastname: Hanson
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Slechta
authors.affiliation: University of Utah Department of Pediatrics, Division of Pediatric Infectious Diseases, Salt Lake City, Utah, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Killpack
authors.affiliation: Intermountain Primary Children's Hospital, Salt Lake City, Utah, USA.
authors.forename: C
authors.initials: C
authors.lastname: Heyrend
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: T
authors.initials: T
authors.lastname: Lunt
authors.affiliation: University of Utah Department of Pathology, Clinical Microbiology, Salt Lake City, Utah, USA Intermountain Primary Children's Hospital, Salt Lake City, Utah, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Daly
authors.affiliation: BioFire Diagnostics, LLC, Salt Lake City, Utah, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Hemmert
authors.affiliation: University of Utah Department of Pediatrics, Division of Pediatric Infectious Diseases, Salt Lake City, Utah, USA kim.hanson@hsc.utah.edu anne.blaschke@hsc.utah.edu.
authors.forename: A J
authors.initials: AJ
authors.lastname: Blaschke
mesh_terms: D001331:Automation; D002494:Central Nervous System Infections / Q000175:diagnosis* / Q000209:etiology*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic