title: Real-time Recordings of Migrating Cortical Neurons from GFP and Cre Recombinase Expressing Mice.
abstract: The cerebral cortex is one of the most intricate regions of the brain that requires elaborate cell migration patterns for its development. Experimental observations show that projection neurons migrate radially within the cortical wall, whereas interneurons migrate along multiple tangential paths to reach the developing cortex. Tight regulation of the cell migration processes ensures proper positioning and functional integration of neurons to specific cerebral cortical circuits. Disruption of neuronal migration often leads to cortical dysfunction and/or malformation associated with neurological disorders. Unveiling the molecular control of neuron migration is thus fundamental to understanding the physiological or pathological development of the cerebral cortex. In this unit, protocols allowing detailed analysis of patterns of migration of both interneurons and projection neurons under different experimental conditions (i.e., loss or gain of function) are presented.
authors.affiliation: GIGA-Neurosciences, University of Liège, Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, Liège, Belgium.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Tielens
authors.affiliation: GIGA-Neurosciences, University of Liège, Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, Liège, Belgium.
authors.forename: Juliette D
authors.initials: JD
authors.lastname: Godin
authors.affiliation: GIGA-Neurosciences, University of Liège, Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, Liège, Belgium.|Walloon Excellence in Life Sciences and Biotechnology (WELBIO), University of Liège, Liège, Belgium.
authors.forename: Laurent
authors.initials: L
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D007395:Interneurons / Q000502:physiology; D049408:Luminescent Agents / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000502:physiology*; D009488:Neurosciences / Q000379:methods*
chemical_list: D049408:Luminescent Agents; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases
keywords: focal electroporation; live imaging; migration; neuron; organotypic slices