title: The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during Arabidopsis seed development.
abstract: The seed expressed gene DELAY OF GERMINATION (DOG) 1 is absolutely required for the induction of dormancy. Next to a non-dormant phenotype, the dog1-1 mutant is also characterized by a reduced seed longevity suggesting that DOG1 may affect additional seed processes as well. This aspect however, has been hardly studied and is poorly understood. To uncover additional roles of DOG1 in seeds we performed a detailed analysis of the dog1 mutant using both transcriptomics and metabolomics to investigate the molecular consequences of a dysfunctional DOG1 gene. Further, we used a genetic approach taking advantage of the weak aba insensitive (abi) 3-1 allele as a sensitized genetic background in a cross with dog1-1. DOG1 affects the expression of hundreds of genes including LATE EMBRYOGENESIS ABUNDANT and HEAT SHOCK PROTEIN genes which are affected by DOG1 partly via control of ABI5 expression. Furthermore, the content of a subset of primary metabolites, which normally accumulate during seed maturation, was found to be affected in the dog1-1 mutant. Surprisingly, the abi3-1 dog1-1 double mutant produced green seeds which are highly ABA insensitive, phenocopying severe abi3 mutants, indicating that dog1-1 acts as an enhancer of the weak abi3-1 allele and thus revealing a genetic interaction between both genes. Analysis of the dog1 and dog1 abi3 mutants revealed additional seed phenotypes and therefore we hypothesize that DOG1 function is not limited to dormancy but that it is required for multiple aspects of seed maturation, in part by interfering with ABA signalling components.
authors.affiliation: Wageningen Seed Laboratory, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB, Wageningen, The Netherlands.|Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708, PB Wageningen, The Netherlands.|Department of Molecular Plant Physiology, Utrecht University, NL-3584 CH, Utrecht, The Netherlands.
authors.forename: Bas J W
authors.initials: BJ
authors.lastname: Dekkers
authors.affiliation: Wageningen Seed Laboratory, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB, Wageningen, The Netherlands.|Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708, PB Wageningen, The Netherlands.
authors.forename: Hanzi
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Molecular Plant Physiology, Utrecht University, NL-3584 CH, Utrecht, The Netherlands.|Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187, Umeå, Sweden.
authors.forename: Johannes
authors.initials: J
authors.lastname: Hanson
authors.affiliation: Wageningen Seed Laboratory, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB, Wageningen, The Netherlands.|Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708, PB Wageningen, The Netherlands.
authors.forename: Leo A J
authors.initials: LA
authors.lastname: Willems
authors.affiliation: Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708, PB Wageningen, The Netherlands.
authors.forename: Diaan C L
authors.initials: DC
authors.lastname: Jamar
authors.affiliation: INRA, Institut Jean-Pierre Bourgin (IJPB), UMR 1318 INRA/AgroParisTech, ERL CNRS 3559, Université Paris-Saclay, 'Saclay Plant Sciences' - RD10, F-78026, Versailles, France.|Chair of Plant Physiology, AgroParisTech, 16 rue Claude Bernard, F-75231, Paris Cedex 05, France.
authors.forename: Gwendal
authors.initials: G
authors.lastname: Cueff
authors.affiliation: INRA, Institut Jean-Pierre Bourgin (IJPB), UMR 1318 INRA/AgroParisTech, ERL CNRS 3559, Université Paris-Saclay, 'Saclay Plant Sciences' - RD10, F-78026, Versailles, France.|Chair of Plant Physiology, AgroParisTech, 16 rue Claude Bernard, F-75231, Paris Cedex 05, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Rajjou
authors.affiliation: Wageningen Seed Laboratory, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB, Wageningen, The Netherlands.|Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708, PB Wageningen, The Netherlands.
authors.forename: Henk W M
authors.initials: HW
authors.lastname: Hilhorst
authors.affiliation: Wageningen Seed Laboratory, Wageningen University, Droevendaalsesteeg 1, NL-6708 PB, Wageningen, The Netherlands.|Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, NL-6708, PB Wageningen, The Netherlands.
authors.forename: Leónie
authors.initials: L
authors.lastname: Bentsink
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018525:Germination; D008954:Models, Biological; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D057445:Plant Dormancy; D010937:Plant Growth Regulators / Q000378:metabolism*; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: C091601:ABI3 protein, Arabidopsis; C409743:ABI5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C532384:DOG1 protein, Arabidopsis; D010937:Plant Growth Regulators; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: ABA insensitive; Arabidopsis thaliana (Arabidopsis); DELAY OF GERMINATION; abscisic acid; dormancy; seed development; seed maturation