title: Efficient butanol recovery from acetone-butanol-ethanol fermentation cultures grown on sweet sorghum juice by pervaporation using silicalite-1 membrane.
abstract: We investigated butanol recovery by pervaporation separation, using a silicalite-1 membrane, from batch cultures of butanol-producing Clostridium beijerinckii SBP2 grown on sweet sorghum juice as a fermentation medium. The pervaporation system yielded 73% (w/v) butanol from intact feed cultures containing 1% (w/v) butanol, and had a butanol permeation flux of 11 g m(-2) h(-1). Upon neutralization and activated charcoal treatment of the feed cultures, butanol yield and total flux increased to 82% (w/v) and 40 g m(-2) h(-1), respectively. This system is applicable to refining processes for practical biobutanol production from a promising energy crop, sweet sorghum.
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; Ibaraki University College of Agriculture, 3-21-1 Chuou, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Kanemoto
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Negishi
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Sakaki
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Ikegami
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; Ibaraki University College of Agriculture, 3-21-1 Chuou, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chohnan
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; Ibaraki University College of Agriculture, 3-21-1 Chuou, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Nitta
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; Ibaraki University College of Agriculture, 3-21-1 Chuou, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan.
authors.forename: Yasurou
authors.initials: Y
authors.lastname: Kurusu
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; Ibaraki University College of Agriculture, 3-21-1 Chuou, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan. Electronic address: hiroyuki.ohta.1494@vc.ibaraki.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
mesh_terms: D020001:1-Butanol / Q000302:isolation & purification* / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D002606:Charcoal; D046970:Clostridium beijerinckii / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008567:Membranes, Artificial; D045868:Sorghum / Q000737:chemistry*; D014835:Volatilization
chemical_list: D008567:Membranes, Artificial; D000096:Acetone; D002606:Charcoal; D000431:Ethanol; D020001:1-Butanol
keywords: Biobutanol; Clostridium beijerinckii; Membrane; Pervaporation; Separation; Sweet sorghum