title: Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis.
abstract: Therapeutic strategies for the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS) have not yet provided satisfactory results. Interest in stem cells for the treatment of neurodegenerative diseases is increasing and their beneficial action seems to be due to a paracrine effect via the release of exosomes, main mediators of cell-cell communication. Here we wished to assess, in vitro, the efficacy of a novel non-cell therapeutic approach based on the use of exosomes derived from murine adipose-derived stromal cells on motoneuron-like NSC-34 cells expressing ALS mutations, and used as in vitro models of disease. In particular, we set out to investigate the effect of exosomes on NSC-34 naïve cells and NSC-34 cells overexpressing human SOD1(G93A) or SOD1(G37R) or SOD1(A4V) mutants, exposed to oxidative stress. The data presented here indicate for the first time that exosomes (0.2 µg/ml) are able to protect NSC-34 cells from oxidative damage, which is one of the main mechanism of damage in ALS, increasing cell viability. These data highlight a promising role of exosomes derived from stem cells for potential therapeutic applications in motoneuron disease.
authors.affiliation: Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Bonafede
authors.affiliation: Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Scambi
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Trento, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Peroni
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Trento, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Potrich
authors.affiliation: Department of Computer Science, University of Verona, Verona, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Boschi
authors.affiliation: Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Benati
authors.affiliation: Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Bonetti
authors.affiliation: Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy. Electronic address: raffaella.mariotti@univr.it.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Mariotti
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009154:Mutation; D018696:Neuroprotective Agents; D018384:Oxidative Stress / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D018696:Neuroprotective Agents; D006861:Hydrogen Peroxide
keywords: ALS; Extracellular vesicles; Mesenchymal stem cell; Motoneuron disease; Motoneuron-like NSC-34; Paracrine effect