title: Upregulated P2X3 Receptor Expression in Patients with Intractable Temporal Lobe Epilepsy and in a Rat Model of Epilepsy.
abstract: Purinergic P2X3 receptors (P2X3Rs) play extensive roles in nerve cells in the central nervous system, particularly in hyperexcitability and calcium (Ca(2+)) influx. However, the role of P2X3Rs in epilepsy has not been previously investigated. To determine the relationship between P2X3Rs and epilepsy, the expression and cellular location of P2X3Rs in patients with intractable temporal lobe epilepsy (TLE) and in a lithium chloride-pilocarpine-induced chronic rat model of epilepsy were assessed. Furthermore, the function of P2X3Rs was assessed in vitro. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis were used to evaluate the expression levels of P2X3Rs in brain tissues from TLE patients and an epileptic rat model, whereas immunofluorescence labeling was applied to determine the distribution of target proteins. Whole-cell recording was subsequently performed to identify the influence of P2X3Rs on seizure-like discharges. P2X3Rs were located at the cell bodies and dendrites of neurons with significantly increased expression in the TLE patients and epileptic rat model. In vitro, P2X3R activation accelerated sustained repetitive firing, whereas P2X3R inhibition led to relatively low-frequency discharges. To the best of our knowledge, this is the first study provide evidence that upregulated P2X3R expression exists in both epileptic humans and rats and may aggravate the epileptic state in vitro. Thus, P2X3Rs may represent a novel therapeutic target for antiepileptic drugs.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Ma
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Jin-Xian
authors.initials: JX
authors.lastname: Yuan
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Ruo-Han
authors.initials: RH
authors.lastname: Li
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China.
authors.forename: Jia-Ni
authors.initials: JN
authors.lastname: Li
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing, 400010, China. chenym1997@sina.com.
authors.forename: Yang-Mei
authors.initials: YM
authors.lastname: Chen
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000293:Adolescent; D000328:Adult; D000818:Animals; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010862:Pilocarpine / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D058477:Receptors, Purinergic P2X3 / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology*; D055815:Young Adult
chemical_list: D058477:Receptors, Purinergic P2X3; D010862:Pilocarpine
keywords: Calcium; Epileptic rat model; Hyperexcitability; P2X3 receptor; Temporal lobe epilepsy