title: Effect of caveolin-1 on the expression of tight junction-associated proteins in rat glioma-derived microvascular endothelial cells.
abstract: Caveolin-1 affects the permeability of blood-tumor barrier (BTB) by regulating the expression of tight junction-associated proteins. However, the effect is still controversial. In the present work, we studied the regulative effect of caveolin-1 on the expression of tight junction-associated proteins and BTB via directly silencing and overexpressing of caveolin-1 by recombinant adenovirus transduction of glioma-derived microvascular endothelial cells in rat brain. The results show that the caveolin-1 downregulation resulted in decreased expression of tight junction-associated proteins, opening of tight junctions, and increasing the permeability of BTB, whereas the overexpression of caveolin-1 presented the opposite effects. Therefore, we conclude that caveolin-1 regulates the expression of tight junction-associated proteins in a positive manner, which further plays a role in the regulation of BTB permeability. This finding provides a novel therapeutic target for selectively opening of BTB.
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University Shenyang 110122, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University Shenyang 110122, PR China.
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Liu
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University Shenyang 110122, PR China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University Shenyang 110122, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University Shenyang 110122, PR China.
authors.forename: Yi-Xue
authors.initials: YX
authors.lastname: Xue
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000648:ultrastructure*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002199:Capillary Permeability / Q000502:physiology; D051242:Caveolin 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017208:Rats, Wistar; D062725:Tight Junction Proteins / Q000096:biosynthesis*
chemical_list: C495537:Cav1 protein, rat; D051242:Caveolin 1; D062725:Tight Junction Proteins
keywords: Caveolin-1; ZO-1; blood-tumor barrier; claudin-5; occludin; tight junction-associated proteins