title: Brucella abortus Omp19 recombinant protein subcutaneously co-delivered with an antigen enhances antigen-specific T helper 1 memory responses and induces protection against parasite challenge.
abstract: The discovery of effective adjuvants for many vaccines especially those with limited commercial appeal, such as vaccines to poverty-related diseases, is required. In this work, we demonstrated that subcutaneous co-administration of mice with the outer membrane protein U-Omp19 from Brucella spp. plus OVA as antigen (Ag) increases Ag-specific T cell proliferation and T helper (Th) 1 immune responses in vitro and in vivo. U-Omp19 treated dendritic cells promote IFN-γ production by specific CD4(+) T cells and increases T cell proliferation. U-Omp19 co-administration induces the production of Ag specific effector memory T cell populations (CD4(+) CD44(high) CD62L(low) T cells). Finally, subcutaneous co-administration of U-Omp19 with Trypanosoma cruzi Ags confers protection against virulent parasite challenge, reducing parasitemia and weight loss while increasing mice survival. These results indicate that the bacterial protein U-Omp19 when delivered subcutaneously could be a suitable component of vaccine formulations against infectious diseases requiring Th1 immune responses.
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires, Argentina.
authors.forename: Lorena M
authors.initials: LM
authors.lastname: Coria
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires, Argentina.
authors.forename: Andrés E
authors.initials: AE
authors.lastname: Ibañez
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires, Argentina.
authors.forename: Karina A
authors.initials: KA
authors.lastname: Pasquevich
authors.affiliation: Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Buenos Aires, Argentina.
authors.forename: Paula L González
authors.initials: PLG
authors.lastname: Cobiello
authors.affiliation: Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Buenos Aires, Argentina.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Frank
authors.affiliation: Instituto de Inmunología, Genética y Metabolismo (INIGEM), Laboratorio de Inmunogenética, Hospital de Clínicas "José de San Martín", CONICET-UBA, Buenos Aires, Argentina.
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Giambartolomei
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires, Argentina. Electronic address: jucassataro@iibintech.com.ar.
authors.forename: Juliana
authors.initials: J
authors.lastname: Cassataro
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002003:Brucella abortus; D002417:Cattle; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007156:Immunologic Memory; D008074:Lipoproteins / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014349:Trypanosoma cruzi
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000953:Antigens, Protozoan; D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; C097319:OMP19 protein, Brucella abortus; D011994:Recombinant Proteins; D010047:Ovalbumin
keywords: Adjuvant; Brucella; Outer membrane protein; T helper 1; Vaccine