title: Anion-selective channelrhodopsin expressed in neuronal cell culture and in vivo in murine brain: Light-induced inhibition of generation of action potentials.
abstract: Anionic channelrhodopsin slow ChloC was expressed in the culture of nerve cells and in vivo in mouse brain. We demonstrated ability of slow ChloC to suppress effectively the activity of the neuron in response to the illumination with the visible light. It has been shown for a first time that slow ChloC works equally efficiently in both neuronal culture and in the whole brain being expressed in vivo. Thus, slow ChloC could be considered as an effective optogenetic tool capable in response to light stimulation to inhibit the generation of action potentials in the neuron.
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russia.|Moscow State University, Faculty of Biology, Moscow, Russia.|Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.affiliation: Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Malyshev
authors.affiliation: Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Salozhin
authors.affiliation: Moscow State University, Faculty of Biology, Moscow, Russia.|Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Nekrasova
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russia.|Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: L E
authors.initials: LE
authors.lastname: Petrovskaya
authors.affiliation: Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Roshchin
authors.affiliation: Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Borodinova
authors.affiliation: Moscow State University, Faculty of Biology, Moscow, Russia.|N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: T B
authors.initials: TB
authors.lastname: Feldman
authors.affiliation: Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: P M
authors.initials: PM
authors.lastname: Balaban
authors.affiliation: Moscow State University, Faculty of Biology, Moscow, Russia.|Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
authors.affiliation: Moscow State University, Faculty of Biology, Moscow, Russia. ostrovsky3535@mail.ru.|N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia. ostrovsky3535@mail.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Ostrovsky
mesh_terms: D000200:Action Potentials; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D008027:Light; D051379:Mice; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D062308:Optogenetics / Q000379:methods; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D012243:Rhodopsin