title: Correlation analysis of cytokine gene expression changes and antiulcer effects of Pro-Gly-Pro and N-Acetyl-Pro-Gly-Pro in experimental models of ulceration.
abstract: Direct correlation of the cytokine gene expression level in peripheral blood mononuclear cells (BMNCs) and gastric mucosa (GM) cells with the development of gastric ulcers of various etiologies was shown for the first time. Ethanol-induced ulceration causes an increased transcription of IFNa, IL-8, and IL-12 mRNA in BMNCs. GM damages caused by water immersion stress were accompanied by an increased transcription of TNFa. The sizes of acetate-induced damages were positively correlated with the expression of IL-10 and IL-8 genes in BMNCs and with the expression of IFNa, IL-2, IL-12, and TNF genes in GM cells. Intranasal administration of Pro-Gly-Pro (PGP) reduced ethanol-induced ulceration, activating the transcription of IFNγ, IL-2, and IL-4 mRNA in BMNCs and prevents the formation of stress- and acetateinduced ulcers by inhibiting the expression of IL-8 and IL-10 genes, respectively.
authors.affiliation: Pirogov Russian National Research Medical University, RF Ministry of Health Care, ul. Ostrovityanova 1, Moscow, 117997, Russia. bakaeva_z@mail.ru.
authors.forename: Z V
authors.initials: ZV
authors.lastname: Bakaeva
authors.affiliation: Pirogov Russian National Research Medical University, RF Ministry of Health Care, ul. Ostrovityanova 1, Moscow, 117997, Russia.
authors.forename: I G
authors.initials: IG
authors.lastname: Kozlov
authors.affiliation: Moscow State University, Moscow, 119991, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Sangadzhieva
authors.affiliation: Moscow State University, Moscow, 119991, Russia.
authors.forename: G E
authors.initials: GE
authors.lastname: Samonina
authors.affiliation: Gamaleya Federal Center of Epidemiology and Microbiology, Russian Academy of Medical Sciences, ul. Gamalei 18, Moscow, 123098, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Mezentseva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Andreeva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Myasoedov
mesh_terms: D000085:Acetates / Q000633:toxicity; D000818:Animals; D000431:Ethanol / Q000633:toxicity; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology* / Q000627:therapeutic use; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000209:etiology; D013315:Stress, Psychological / Q000150:complications; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D016898:Interferon-alpha; D007378:Interleukins; D009842:Oligopeptides; D014409:Tumor Necrosis Factor-alpha; C113164:prolyl-glycyl-proline; D000431:Ethanol; D011392:Proline