title: Clinical characterization for proliferation and metastasis in advanced hepatocellular carcinoma patients.
abstract: Here we reported that association between drug resistance and carcinomatosis in advanced liver cancer cases. All subjects (n=4) were periodically received chemotherapeutic agents when clinical manifestation being defined serologically. Hepatic specimen was harvested via biopsy and further prepared as paraffin-slice before conducting immunohistochemistry. As a consequence, more detectable biomarkers, such as AST, AFP, GGT2, were high expressed in plasma when compared to clinical standards. DNA topoisomerase II (TOPO II), Ki-67 were immunoreactively labeled in cytoplasm/membrane and nucleolus of liver cancer cells, while hepatocellular tumor protein p53 was negative or non-detected. Additionally, we found that hepatobiliary cancer showed epithelial differentiation with pronounced CK19 immunoreactivity when metastasizing. Our clinicopathologic findings demonstrate that correlation between carcinomatous proliferation/metastasis and drug resistance protein expression. Furthermore, these evidences indicate that TOPO II may be a biomarker for advanced hepatocellular carcinoma patient receiving chemotherapeutics.
authors.affiliation: Department of Pathology, Guigang City People's Hospital, The Eighth Affiliated Hospital of Guangxi Medical University Guigang 537100, Guangxi, PR China.
authors.forename: Cuirong
authors.initials: C
authors.lastname: Wei
authors.affiliation: Department of Oncology, Guigang City People's Hospital, The Eighth Affiliated Hospital of Guangxi Medical University Guigang 537100, Guangxi, PR China.
authors.forename: Qijin
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Department of Pharmacy, The Second People's Hospital of Nanning City Nanning 530031, Guangxi, PR China.
authors.forename: Ka
authors.initials: K
authors.lastname: Wu
authors.affiliation: Guilin Medical University Guilin 541004, Guangxi, PR China ; Department of Biology, Hong Kong Baptist University Hong Kong, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D049109:Cell Proliferation; D004250:DNA Topoisomerases, Type II / Q000032:analysis / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D004250:DNA Topoisomerases, Type II
keywords: Hepatocellular carcinoma; biomarker; metastasis; proliferation