title: Generation of Arbas Cashmere Goat Induced Pluripotent Stem Cells Through Fibroblast Reprogramming.
abstract: Various factors affect the process of obtaining stable Arbas cashmere goat embryonic stem cells (ESCs), for example, the difficulty in isolating cells at the appropriate stage of embryonic development, the in vitro culture environment, and passage methods. With the emergence of induced pluripotent stem cell (iPSC) technology, it has become possible to use specific genes to induce somatic cell differentiation in PSCs. We transferred OCT4, SOX2, c-MYC, and KLF4 into Arbas cashmere goat fetal fibroblasts, then induced and cultured them using a drug-inducible system to obtain Arbas goat iPSCs that morphologically resembled mouse iPSCs. After identification, the obtained goat iPSCs expressed ESC markers, had a normal karyotype, could differentiate into embryoid bodies in vitro, and could differentiate into three germ layer cell types and form teratomas in vivo. We used microarray gene expression profile analysis to elucidate the reprogramming process. Our results provide the experimental basis for establishing cashmere goat iPSC lines and for future in-depth studies on molecular mechanism of cashmere goat somatic cell reprogramming.
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .|2 These authors contributed equally to this work.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Tai
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .|2 These authors contributed equally to this work.
authors.forename: Pengxia
authors.initials: P
authors.lastname: Liu
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .
authors.forename: Muzi
authors.initials: M
authors.lastname: Jin
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .
authors.forename: Teng
authors.initials: T
authors.lastname: Xu
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: 1 Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University , Inner Mongolia, Hohhot, 010021, China .
authors.forename: Dongjun
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D065150:Cellular Reprogramming; D005333:Fetus / Q000166:cytology* / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D006041:Goats / Q000196:embryology* / Q000502:physiology; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D000090062:Kruppel-Like Factor 4; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D019076:Transgenes