title: Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest.
abstract: Recently, a lectin was purified from the potato cultivated in Bangladesh locally known as Sheel. In the present study cytotoxicity of the lectin against Ehrlich ascites carcinoma (EAC) cells was studied by MTT assay in vitro in RPMI-1640 medium and 8.0-36.0 % cell growth inhibition was observed at the range of 2.5-160 μg/ml protein concentration when incubated for 24 h. The lectin-induced apoptosis in EAC cells was confirmed by fluorescence and optical microscope. The apoptotic cell death was also confirmed by using caspase inhibitors. Cells growth inhibition caused by the lectin (36 %) was remarkably decreased to 7.6 and 22.3 % respectively in the presence of caspase-3 and -8 inhibitors. RT-PCR was used to evaluate the expression of apoptosis-related genes Bcl-X, p53, and Bax. An intensive expression of Bcl-X gene was observed in untreated control EAC cells with the disappeared of the gene in Sheel-treated EAC cells. At the same time, Bax gene expression appeared only in Sheel-treated EAC cells and the expression level of the p53 gene was increased remarkable after the treatment of EAC cells with the lectin. The lectin showed strong agglutination activity against EAC cells. Flow cytometry was used to study the cell cycle phases of EAC cells and it was observed that the lectin arrested the G2/M phase. In conclusion, Sheel lectin inhibited EAC cells growth by inducing apoptosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science, Rajshahi University, Rajshahi, 6205, Bangladesh. rashelkabir@ru.ac.bd.
authors.forename: Syed Rashel
authors.initials: SR
authors.lastname: Kabir
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science, Rajshahi University, Rajshahi, 6205, Bangladesh.
authors.forename: Md Musfikur
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Bangladesh Council of Scientific and Industrial Research Laboratories, Rajshahi, Bangladesh.
authors.forename: Ruhul
authors.initials: R
authors.lastname: Amin
authors.affiliation: IFST, Bangladesh Council of Scientific and Industrial Research Laboratories, Dhaka, Bangladesh.
authors.forename: Md Rezaul
authors.initials: MR
authors.lastname: Karim
authors.affiliation: Environmental Microbiology Laboratory, icddr,b, 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.
authors.forename: Zahid Hayat
authors.initials: ZH
authors.lastname: Mahmud
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science, Rajshahi University, Rajshahi, 6205, Bangladesh.
authors.forename: M Tofazzal
authors.initials: MT
authors.lastname: Hossain
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D051379:Mice; D037121:Plant Lectins / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D037121:Plant Lectins; C041202:potato lectin
keywords: Agglutination; Apoptosis; Caspase inhibitors; Cell cycle; Gene expression; Lectin