title: Development of a colloidal gold immunochromatographic strip assay for simple and fast detection of human α-lactalbumin in genetically modified cow milk.
abstract: The qualitative and quantitative declaration of food ingredients is important to consumers, especially for genetically modified food as it experiences a rapid increase in sales. In this study, we designed an accurate and rapid detection system using colloidal gold immunochromatographic strip assay (GICA) methods to detect genetically modified cow milk. First, we prepared 2 monoclonal antibodies for human α-lactalbumin (α-LA) and measured their antibody titers; the one with the higher titer was used for further experiments. Then, we found the optimal pH value and protein amount of GICA for detection of pure milk samples. The developed strips successfully detected genetically modified cow milk and non-modified cow milk. To determine the sensitivity of GICA, a quantitative ELISA system was used to determine the exact amount of α-LA, and then genetically modified milk was diluted at different rates to test the sensitivity of GICA; the sensitivity was 10 μg/mL. Our results demonstrated that the applied method was effective to detect human α-LA in cow milk.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Wuhan 430070, Hubei, PR China.
authors.forename: Chenyu
authors.initials: C
authors.lastname: Tao
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Qingde
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Wuhan 430070, Hubei, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Deshi
authors.initials: D
authors.lastname: Shi
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Wuhan 430070, Hubei, PR China. Electronic address: liubang@mail.hzau.edu.cn.
authors.forename: Bang
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D017979:Gold Colloid; D006801:Humans; D007768:Lactalbumin / Q000032:analysis* / Q000276:immunology; D008892:Milk / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D007768:Lactalbumin
keywords: ELISA; colloidal gold immunochromatographic strip assay; genetically modified cow milk; human α-lactalbumin