title: Drosophila MOF regulates DIAP1 and induces apoptosis in a JNK dependent pathway.
abstract: Histone modulations have been implicated in various cellular and developmental processes where in Drosophila Mof is involved in acetylation of H4K16. Reduction in the size of larval imaginal discs is observed in the null mutants of mof with increased apoptosis. Deficiency involving Hid, Reaper and Grim [H99] alleviated mof (RNAi) induced apoptosis in the eye discs. mof (RNAi) induced apoptosis leads to activation of caspases which is suppressed by over expression of caspase inhibitors like P35 and Diap1 clearly depicting the role of caspases in programmed cell death. Also apoptosis induced by knockdown of mof is rescued by JNK mutants of bsk and tak1 indicating the role of JNK in mof (RNAi) induced apoptosis. The adult eye ablation phenotype produced by ectopic expression of Hid, Rpr and Grim, was restored by over expression of Mof. Accumulation of Mof at the Diap1 promoter 800 bp upstream of the transcription start site in wild type larvae is significantly higher (up to twofolds) compared to mof (1) mutants. This enrichment coincides with modification of histone H4K16Ac indicating an induction of direct transcriptional up regulation of Diap1 by Mof. Based on these results we propose that apoptosis triggered by mof (RNAi) proceeds through a caspase-dependent and JNK mediated pathway.
authors.affiliation: Agricultural Research Station, Prof. Jayashankar Telangana State Agricultural University, Tandur, 501141, Telangana, India.
authors.forename: Sreerangam N C V L
authors.initials: SN
authors.lastname: Pushpavalli
authors.affiliation: Centre for Chemical Biology, Indian Institute of Chemical Technology, Uppal Road, Hyderabad, 500007, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Sarkar
authors.affiliation: Centre for Chemical Biology, Indian Institute of Chemical Technology, Uppal Road, Hyderabad, 500007, India.|School of Chemical and Biotechnology (SCBT), SASTRA University, Tirumalaisamudram, Thanjavur, Tamil Nadu, 613401, India.
authors.forename: M Janaki
authors.initials: MJ
authors.lastname: Ramaiah
authors.affiliation: Centre for Chemical Biology, Indian Institute of Chemical Technology, Uppal Road, Hyderabad, 500007, India.
authors.forename: G
authors.initials: G
authors.lastname: Koteswara Rao
authors.affiliation: Centre for Chemical Biology, Indian Institute of Chemical Technology, Uppal Road, Hyderabad, 500007, India.|Functional Genomics and Gene Silencing Group, Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India.
authors.forename: Indira
authors.initials: I
authors.lastname: Bag
authors.affiliation: Functional Genomics and Gene Silencing Group, Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India.
authors.forename: Utpal
authors.initials: U
authors.lastname: Bhadra
authors.affiliation: Centre for Chemical Biology, Indian Institute of Chemical Technology, Uppal Road, Hyderabad, 500007, India. manikapb@gmail.com.
authors.forename: Manika
authors.initials: M
authors.lastname: Pal-Bhadra
mesh_terms: D000107:Acetylation; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D005123:Eye / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D060227:Imaginal Discs / Q000002:abnormalities* / Q000166:cytology; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D007814:Larva; D020930:MAP Kinase Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: C111891:DIAP1 protein, Drosophila; D029721:Drosophila Proteins; C108880:HID protein, Drosophila; D006657:Histones; D051034:Inhibitor of Apoptosis Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; C108881:grim protein, Drosophila; C086973:rpr protein, Drosophila; D051548:Histone Acetyltransferases; C111063:mof protein, Drosophila; D020930:MAP Kinase Kinase Kinases; C000593602:TAK1 protein, Drosophila; D020169:Caspases
keywords: Apoptosis; Diap1; Diap1 promoter; Grim; H4K16Ac; Hid; Mof; Reaper