title: Apigenin-7-O-β-D-(-6''-p-coumaroyl)-Glucopyranoside Treatment Elicits Neuroprotective Effect against Experimental Ischemic Stroke.
abstract: Stroke is the major cause of permanent disability and mortality in China. Apigenin-7-O-β-D-(-6''-p-coumaroyl)-glucopyranoside (APG) is a glycoside subtype of apigenin and has the antioxidant activity; however, whether and how it plays a neuroprotective role following cerebral ischemia remains unknown. In present study, we adopted the oxygen glucose/reperfusion model in PC12 cells, bilateral common carotid artery occlusion model in C57B6 mice and middle cerebral artery occlusion model in SD rats to observe the therapeutic effects of APG on ischemic stroke. We also discussed the underlying mechanism. Treatment with 0.4 μg/ml or 0.8 μg/ml APG promoted cell viability and proliferation, reduced LDH release and apoptotic cell death levels in PC12 cells. Treatment with 50 mg/kg or 100 mg/kg APG at 30 minutes after reperfusion improved neurological outcomes in vivo, as demonstrated by elevation of neurological scores in both mice and rats. It also increased the number of survival neurons in mice and reduced infarct volume in rats. APG also increased the contents of Mn-SOD and the phosphorylation level of STAT3, elevated the antioxidant activity and reduced oxidative productions. These findings revealed a neuroprotective effect of APG, which possibly induced by the STAT3 phosphorylation-mediated Mn-SOD up-regulation.
authors.affiliation: 1. Department of Psychiatry;
authors.forename: Min
authors.initials: M
authors.lastname: Cai
authors.affiliation: 2. Department of Anesthesiology;
authors.forename: Yulong
authors.initials: Y
authors.lastname: Ma
authors.affiliation: 3. Department of Pharmacology; Xijing hospital, the Forth Military Medical School.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: 2. Department of Anesthesiology;
authors.forename: Shiquan
authors.initials: S
authors.lastname: Wang
authors.affiliation: 1. Department of Psychiatry;
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 2. Department of Anesthesiology;
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.affiliation: 1. Department of Psychiatry;
authors.forename: Zhengwu
authors.initials: Z
authors.lastname: Peng
authors.affiliation: 1. Department of Psychiatry;
authors.forename: Huaning
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1. Department of Psychiatry;
authors.forename: Tan
authors.initials: T
authors.lastname: Qingrong
mesh_terms: D000818:Animals; D047310:Apigenin / Q000627:therapeutic use*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000201:enzymology; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival; D005434:Flow Cytometry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000188:drug therapy* / Q000201:enzymology
chemical_list: D018696:Neuroprotective Agents; D047310:Apigenin; D007770:L-Lactate Dehydrogenase
keywords: Apigenin-7-O-β-D-(-6''-p-coumaroyl)-glucopyranoside (APG); cerebral ischemia/reperfusion; manganese-superoxide dismutase; oxidative stress