title: Prime-booster vaccination of cattle with an influenza viral vector Brucella abortus vaccine induces a long-term protective immune response against Brucella abortus infection.
abstract: This study analyzed the duration of the antigen-specific humoral and T-cell immune responses and protectiveness of a recently-developed influenza viral vector Brucella abortus (Flu-BA) vaccine expressing Brucella proteins Omp16 and L7/L12 and containing the adjuvant Montadine Gel01 in cattle. At 1 month post-booster vaccination (BV), both humoral (up to 3 months post-BV; GMT IgG ELISA titer 214±55 to 857±136, with a prevalence of IgG2a over IgG1 isotype antibodies) and T-cell immune responses were observed in vaccinated heifers (n=35) compared to control animals (n=35, injected with adjuvant/PBS only). A pronounced T-cell immune response was induced and maintained for 12 months post-BV, as indicated by the lymphocyte stimulation index (2.7±0.4 to 10.1±0.9 cpm) and production of IFN-γ (13.7±1.7 to 40.0±3.0 ng/ml) at 3, 6, 9, and 12 months post-BV. Prime-boost vaccination provided significant protection against B. abortus infection at 3, 6, 9 and 12 months (study duration) post-BV (7 heifers per time point; alpha=0.03-0.01 vs. control group). Between 57.1 and 71.4% of vaccinated animals showed no signs of B. abortus infection (or Brucella isolation) at 3, 6, 9 and 12 months post-BV; the severity of infection, as indicated by the index of infection (P=0.0003 to <0.0001) and rates of Brucella colonization (P=0.03 to <0.0001), was significantly lower for vaccinated diseased animals than appropriate control animals. Good protection from B. abortus infection was also observed among pregnant vaccinated heifers (alpha=0.03), as well as their fetuses and calves (alpha=0.01), for 12 months post-BV. Additionally, 71.4% of vaccinated heifers calved successfully whereas all pregnant control animals aborted (alpha=0.01). Prime-boost vaccination of cattle with Flu-BA induces an antigen-specific humoral and pronounced T cell immune response and most importantly provides good protectiveness, even in pregnant heifers, for at least 12 months post-BV.
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan. Electronic address: tabynov_81@mail.ru.
authors.forename: Kaissar
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Bolat
authors.initials: B
authors.lastname: Yespembetov
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Sholpan
authors.initials: S
authors.lastname: Ryskeldinova
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Zinina
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Zhailaubay
authors.initials: Z
authors.lastname: Kydyrbayev
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Yerken
authors.initials: Y
authors.lastname: Kozhamkulov
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Dulat
authors.initials: D
authors.lastname: Inkarbekov
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Abylai
authors.initials: A
authors.lastname: Sansyzbay
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002004:Brucella Vaccine / Q000627:therapeutic use*; D002003:Brucella abortus; D002007:Brucellosis, Bovine / Q000517:prevention & control*; D002417:Cattle; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007117:Immunization, Secondary / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D053118:Influenza A Virus, H1N1 Subtype; D053124:Influenza A Virus, H5N1 Subtype; D007371:Interferon-gamma / Q000276:immunology; D011247:Pregnancy; D012269:Ribosomal Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12; D007371:Interferon-gamma
keywords: Antibody and T-cell-mediated immune response; Brucella abortus; Cattle; Influenza viral vector; Protectiveness; Vaccine