title: Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice.
abstract: To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300 mg/kg body weight) for 14 days and were injected with saline or LPS at 15 days. Intestinal samples were collected at 4 h post-challenge. The results showed that ASPS ameliorated LPS-induced deterioration of digestive ability of LPS-challenged mice, indicated by an increase in intestinal lactase activity (45%, p<0.05), and the intestinal morphology, as proved by improved villus height (20.84%, p<0.05) and villus height:crypt depth ratio (42%, p<0.05), and lower crypt depth in jejunum (15.55%, p<0.05), as well as enhanced intestinal tight junction proteins expression involving occludin-1 (71.43%, p<0.05). ASPS also prevented intestinal inflammation response, supported by decrease in intestinal inflammatory mediators including tumor necrosis factor α (22.28%, p<0.05) and heat shock protein (HSP70) (77.42%, p<0.05). In addition, intestinal mucus layers were also improved by ASPS, as indicated by the increase in number of goblet cells (24.89%, p<0.05) and intestinal trefoil peptide (17.75%, p<0.05). Finally, ASPS facilitated mRNA expression of epidermal growth factor (100%, p<0.05) and its receptor (200%, p<0.05) gene. These results indicate that ASPS can prevent intestinal mucosal barrier injury under inflammatory conditions, which may be associated with up-regulating gene mRNA expression of epidermal growth factor and its receptor.
authors.affiliation: College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, China .
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, China .
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, China .
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology Research, Liaoning Academy of Agricultural Sciences, Shenyang 1108-66, China .
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, China .
authors.forename: Zishan
authors.initials: Z
authors.lastname: Bai
authors.affiliation: College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, China .
authors.forename: Lianquan
authors.initials: L
authors.lastname: Bian
keywords: Epidermal Growth Factor; Herbal Extract; Inflammation; Intestinal Barrier; Metabolism; Pro-inflammatory Cytokines