title: Proteomics analysis of a long-term survival strain of Escherichia coli K-12 exhibiting a growth advantage in stationary-phase (GASP) phenotype.
abstract: The aim of this work was the functional and proteomic analysis of a mutant, W3110 Bgl(+) /10, isolated from a batch culture of an Escherichia coli K-12 strain maintained at room temperature without addition of nutrients for 10 years. When the mutant was evaluated in competition experiments in co-culture with the wild-type, it exhibited the growth advantage in stationary phase (GASP) phenotype. Proteomes of the GASP mutant and its parental strain were compared by using a 2DE coupled with MS approach. Several differentially expressed proteins were detected and many of them were successful identified by mass spectrometry. Identified expression-changing proteins were grouped into three functional categories: metabolism, protein synthesis, chaperone and stress responsive proteins. Among them, the prevalence was ascribable to the "metabolism" group (72%) for the GASP mutant, and to "chaperones and stress responsive proteins" group for the parental strain (48%).
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Assunta
authors.initials: A
authors.lastname: Gagliardi
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Egidio
authors.initials: E
authors.lastname: Lamboglia
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Bianchi
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Landi
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Armini
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Ciolfi
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Marri
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D048168:Escherichia coli K12 / Q000378:metabolism* / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome
keywords: Aging; Escherichia coli; GASP; Mass spectrometry; Microbiology