title: Gene expression profiles in the intestine of lipopolysaccharide-challenged piglets.
abstract: Bowel diseases are common in human and animals and are characterized by intestinal dysfunction and injury. A well-established porcine model of intestinal injury can be induced by lipopolysaccharide (LPS), an endotoxin released from the cell wall of pathogenic bacteria. LPS affects the expression of genes associated with intestinal immune response, mucosal growth, energy metabolism, absorption, mucosal barrier function, and antiviral function. Transcriptional analysis of intestinal genes reveals that the duodenum, jejunum, ileum and colon respond to LPS challenge in a similar pattern. Moreover, the jejunum and ileum exhibit greater responses to LPS challenge than the duodenum and colon with regard to gene expression. Additionally, over 85% of genes are co-expressed along the small and large intestines and there is a clear distinction in gene expression patterns amongst the different intestinal segments in pigs. These findings have important implications for underlying molecular mechanisms responsible for endotoxin-induced intestinal injury and dysfunction.
authors.affiliation: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University.
authors.forename: Dan
authors.initials: D
authors.lastname: Yi
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China, houyq777@yahoo.com.cn.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Zhao
authors.forename: Binying
authors.initials: B
authors.lastname: Ding
authors.affiliation: Institute of Digestive Disease, Longhua Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China, 2Department of Animal Science, Texas A&M University, College Station.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chen
authors.forename: Yulan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Kang
authors.affiliation: Department of Animal Science, College Station, TX, USA 77843-2471.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000033:anatomy & histology; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D013552:Swine
chemical_list: D008070:Lipopolysaccharides