title: Schizophrenia-Associated MIR204 Regulates Noncoding RNAs and Affects Neurotransmitter and Ion Channel Gene Sets.
abstract: As regulators of gene expression, microRNAs (miRNAs) are likely to play an important role in the development of disease. In this study we present a large-scale strategy to identify miRNAs with a role in the regulation of neuronal processes. Thereby we found variant rs7861254 located near the MIR204 gene to be significantly associated with schizophrenia. This variant resulted in reduced expression of miR-204 in neuronal-like SH-SY5Y cells. Analysis of the consequences of the altered miR-204 expression on the transcriptome of these cells uncovered a new mode of action for miR-204, being the regulation of noncoding RNAs (ncRNAs), including several miRNAs, such as MIR296. Furthermore, pathway analysis showed downstream effects of miR-204 on neurotransmitter and ion channel related gene sets, potentially mediated by miRNAs regulated through miR-204.
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Applied Molecular Genomics Unit, Department of Molecular Genetics, VIB, Antwerp, Belgium.
authors.forename: Sophia
authors.initials: S
authors.lastname: Cammaerts
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Applied Molecular Genomics Unit, Department of Molecular Genetics, VIB, Antwerp, Belgium.
authors.forename: Mojca
authors.initials: M
authors.lastname: Strazisar
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Centralized Service Facility, Department of Molecular Genetics, VIB, Antwerp, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Smets
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Neurogenetics Group, Department of Molecular Genetics, VIB, Antwerp, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Weckhuysen
authors.affiliation: Division of Psychiatry, Department of Clinical Sciences, Umeå University, Umeå, Sweden.
authors.forename: Annelie
authors.initials: A
authors.lastname: Nordin
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Neurogenetics Group, Department of Molecular Genetics, VIB, Antwerp, Belgium.|Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: De Jonghe
authors.affiliation: Division of Psychiatry, Department of Clinical Sciences, Umeå University, Umeå, Sweden.
authors.forename: Rolf
authors.initials: R
authors.lastname: Adolfsson
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Applied Molecular Genomics Unit, Department of Molecular Genetics, VIB, Antwerp, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: De Rijk
authors.affiliation: University of Antwerp, Antwerp, Belgium.|Applied Molecular Genomics Unit, Department of Molecular Genetics, VIB, Antwerp, Belgium.|Multiplicom N.V., Niel, Belgium.
authors.forename: Jurgen
authors.initials: J
authors.lastname: Del Favero
mesh_terms: D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D018377:Neurotransmitter Agents / Q000235:genetics*; D009928:Organ Specificity; D012559:Schizophrenia / Q000235:genetics*
chemical_list: D007473:Ion Channels; C549404:MIRN204 microRNA, human; C588068:MIRN618 microRNA, human; D035683:MicroRNAs; D018377:Neurotransmitter Agents