title: TGF-β1 Upregulates the Expression of Triggering Receptor Expressed on Myeloid Cells 1 in Murine Lungs.
abstract: Triggering receptor expressed on myeloid cells 1 (TREM-1) increases the expression of TGF-β family genes, which are known as profibrogenic cytokines in the pathogenesis of pulmonary fibrosis. In this study, we determined whether TGF-β1 regulated the expression of TREM-1 in a mouse model of pulmonary fibrosis. The expression of TGF-β1 and TREM-1 was increased on day 7, 14, and 21 after single intratracheal injection of bleomycin (BLM). And there was positive correlation between the expression of TGF-β1 and TREM-1. TGF-β1 increased expression of TREM-1 mRNA and protein in a time- and dose-dependent manner in mouse macrophages. The expression of the activator protein 1 (AP-1) was increased in lung tissues from mouse after BLM injection and in mouse macrophages after TGF-β1 treatment, respectively. TGF-β1 significantly increased the relative activity of luciferase in the cells transfected with plasmid contenting wild type-promoter of TREM-1. But TGF-β1 had no effect on the activity of luciferase in the cells transfected with a mutant-TREM1 plasmid carrying mutations in the AP-1 promoter binding site. In conclusion, we found the expression of TREM-1 was increased in lung tissues from mice with pulmonary fibrosis. TGF-β1 increased the expression of TREM-1 in mouse macrophages partly via the transcription factor AP-1.
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Rui-Qi
authors.initials: RQ
authors.lastname: Chen
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Sun
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Wen-Zhuo
authors.initials: WZ
authors.lastname: Ran
authors.affiliation: Department of Neurology, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Xiang
authors.initials: X
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Trauma, Burns, and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Jiang
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Cha-Xiang
authors.initials: CX
authors.lastname: Guan
mesh_terms: D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D051379:Mice; D011658:Pulmonary Fibrosis / Q000276:immunology / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D053773:Transforming Growth Factor beta1 / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D011971:Receptors, Immunologic; D018808:Transcription Factor AP-1; D053773:Transforming Growth Factor beta1; C471763:Treml1 protein, mouse