title: Human Urine-derived Stem Cells Seeded Surface Modified Composite Scaffold Grafts for Bladder Reconstruction in a Rat Model.
abstract: We conducted this study to investigate the synergistic effect of human urine-derived stem cells (USCs) and surface modified composite scaffold for bladder reconstruction in a rat model. The composite scaffold (Polycaprolactone/Pluronic F127/3 wt% bladder submucosa matrix) was fabricated using an immersion precipitation method, and heparin was immobilized on the surface via covalent conjugation. Basic fibroblast growth factor (bFGF) was loaded onto the heparin-immobilized scaffold by a simple dipping method. In maximal bladder capacity and compliance analysis at 8 weeks post operation, the USCs-scaffold(heparin-bFGF) group showed significant functional improvement (2.34 ± 0.25 mL and 55.09 ± 11.81 µL/cm H2O) compared to the other groups (2.60 ± 0.23 mL and 56.14 ± 9.00 µL/cm H2O for the control group, 1.46 ± 0.18 mL and 34.27 ± 4.42 µL/cm H2O for the partial cystectomy group, 1.76 ± 0.22 mL and 35.62 ± 6.69 µL/cm H2O for the scaffold group, and 1.92 ± 0.29 mL and 40.74 ± 7.88 µL/cm H2O for the scaffold(heparin-bFGF) group, respectively). In histological and immunohistochemical analysis, the USC-scaffold(heparin-bFGF) group showed pronounced, well-differentiated, and organized smooth muscle bundle formation, a multi-layered and pan-cytokeratin-positive urothelium, and high condensation of submucosal area. The USCs seeded scaffold(heparin-bFGF) exhibits significantly increased bladder capacity, compliance, regeneration of smooth muscle tissue, multi-layered urothelium, and condensed submucosa layers at the in vivo study.
authors.affiliation: Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Jun Nyung
authors.identifier: 0000-0002-6342-9846
authors.initials: JN
authors.lastname: Lee
authors.affiliation: Bio-Medical Research Institute, Kyungpook National University Hospital, Daegu, Korea.
authors.forename: So Young
authors.identifier: 0000-0003-4500-4956
authors.initials: SY
authors.lastname: Chun
authors.affiliation: Bio-Medical Research Institute, Kyungpook National University Hospital, Daegu, Korea.
authors.forename: Hyo-Jung
authors.identifier: 0000-0002-1179-0373
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Neural Development and Disease, Korea Brain Research Institute, Daegu, Korea.
authors.forename: Yu-Jin
authors.identifier: 0000-0001-5660-3001
authors.initials: YJ
authors.lastname: Jang
authors.affiliation: Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Seock Hwan
authors.identifier: 0000-0003-3796-2601
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Laboratory Animal Research Support Team, Yeungnam University, Daegu, Korea.
authors.forename: Dae Hwan
authors.identifier: 0000-0002-2083-0154
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Nanobiomedical Science & WCU Research Center, Dankook University, Cheonan, Korea.
authors.forename: Se Heang
authors.identifier: 0000-0002-4635-6809
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Urology, Yeungnam University College of Medicine, Daegu, Korea.
authors.forename: Phil Hyun
authors.identifier: 0000-0002-3801-258X
authors.initials: PH
authors.lastname: Song
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, Korea.
authors.forename: Jin Ho
authors.identifier: 0000-0002-1528-3416
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Emergency Medicine, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Jong Kun
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Tae Gyun
authors.identifier: 0000-0002-4390-0952
authors.initials: TG
authors.lastname: Kwon
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage; D006493:Heparin / Q000008:administration & dosage; D006801:Humans; D008422:Materials Testing; D023421:Models, Animal; D020442:Poloxamer; D011091:Polyesters; D051381:Rats; D019651:Plastic Surgery Procedures; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D001743:Urinary Bladder / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D014556:Urine / Q000166:cytology*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D016222:Fibroblast Growth Factor 2; D020442:Poloxamer; C016240:polycaprolactone; D006493:Heparin
keywords: Basic Fibroblast Growth Factor 2; Bladder Regeneration; Surface Modified Scaffold; Urine-derived Stem Cells