title: Comparative evaluation of PLGA nanoparticle delivery system for 5-fluorouracil and curcumin on squamous cell carcinoma.
abstract: OBJECTIVE
  The purpose of this study is to assess the effect of 5-fluorouracil nanoparticles and curcumin naoparticles on cell proliferation and the expression of the apoptotic marker (caspase 3) in squamous cell carcinoma cell line.
  
  
  METHODS
  PLGA 5-fluorouracil nanopartciles and PLGA curcumin nanoparticles were prepared and applied for 24 and 48h on human laryngeal squamous carcinoma cell line (Hep-2) as regard IC 50 concentration. MTT assay was used for evaluation of cytotoxicity of prepared nanoparticles. Quantitaive reverse transcriptase polymerase chain reaction (QRT-PCR) was used for the assessment of caspase-3 expression in the treated cell line.
  
  
  RESULTS
  The drug release rate profiles was dependent upon polymer to drug ratio, noting that the higher PLGA polymer ratio to 5-fluprouracil or curcumin drug showed faster release rates. On the other hand, the least PLGA polymer ratio to 5-fluprouracil or curcumin drug showed the slowest release rates. MTT assay revelaed that 5-fluorouracil nanoparticels or curcumin nanoparticels showed a clear cytotoxic effect on Hep-2 cell line compared to non treated cancer cells. The RT-PCR assessment of caspase-3 expression revealed that there was a significant increase in caspase-3 expression in Hep-2 cell line treated with 5-fluorouracil nanoparticles or curcumin compared to non treated cancer cells.
  
  
  CONCLUSIONS
  Curcumin nanoparticles could be more active in inducing apoptosis in short term assays (24h) than long term assays (48h) due to differential cellular uptake. While 5-fluorouracil nanoparticles induced higher significant apoptosis in long term (48h) compared to curcumin group.
authors.affiliation: Oral Pathology Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
authors.forename: Shaimaa M
authors.initials: SM
authors.lastname: Masloub
authors.affiliation: Oral Pathology Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Elmalahy
authors.affiliation: Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt. Electronic address: dinasabry@kasralainy.edu.eg.
authors.forename: Dina
authors.initials: D
authors.lastname: Sabry
authors.affiliation: Polymer and Pigment, National Research Centre, Cairo, Egypt.
authors.forename: Wael S
authors.initials: WS
authors.lastname: Mohamed
authors.affiliation: Lab Technology Department, Faculty of Applied Medical Science, Misr University For Science &Technology, Egypt.
authors.forename: Sahar H
authors.initials: SH
authors.lastname: Ahmed
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004354:Drug Screening Assays, Antitumor; D005472:Fluorouracil / Q000008:administration & dosage* / Q000737:chemistry; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage*; D007822:Laryngeal Neoplasms / Q000188:drug therapy*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D053148:Caspase 3; D003474:Curcumin; D005472:Fluorouracil
keywords: Apoptosis; Caspase-3; Hep-2; Nanotechnology