title: Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation.
abstract: To evaluate the clinical potential of high nitrogen nickel-free austenitic stainless steel (HNNF SS), we have compared the cellular and molecular responses of human umbilical artery smooth muscle cells (HUASMCs) to HNNF SS and 316L SS (nickel-containing austenitic 316L stainless steel). CCK-8 analysis and flow cytometric analysis were used to assess the cellular responses (proliferation, apoptosis, and cell cycle), and quantitative real-time PCR (qRT-PCR) was used to analyze the gene expression profiles of HUASMCs exposed to HNNF SS and 316L SS, respectively. CCK-8 analysis demonstrated that HUASMCs cultured on HNNF SS proliferated more slowly than those on 316L SS. Flow cytometric analysis revealed that HNNF SS could activate more cellular apoptosis. The qRT-PCR results showed that the genes regulating cell apoptosis and autophagy were up-regulated on HNNF SS. Thus, HNNF SS could reduce the HUASMC proliferation in comparison to 316L SS. The findings furnish valuable information for developing new biomedical materials for stent implantation.
authors.affiliation: Institute of Biotechnology, Northeastern University, Shenyang, China.|Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: An
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Yifu
authors.initials: Y
authors.lastname: Guan
mesh_terms: D017209:Apoptosis; D015415:Biomarkers; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D009532:Nickel; D013193:Stainless Steel; D015607:Stents; D013499:Surface Properties; D059467:Transcriptome; D014469:Umbilical Arteries / Q000166:cytology*
chemical_list: D015415:Biomarkers; C426023:austenitic steel; D013193:Stainless Steel; D009532:Nickel