title: Efficacy of Dietary Lipid Control in Healing High-Fat and High-Cholesterol Diet-Induced Fibrotic Steatohepatitis in Rats.
abstract: Nonalcoholic steatohepatitis is related to lifestyle, particularly to dietary habits. We developed diet-induced fibrotic steatohepatitis model stroke-prone spontaneously hypertensive 5/Dmcr (SHRSP5/Dmcr) rats showing steatosis, hepatic inflammation, and severe fibrosis induced by high-fat and -cholesterol (HFC) diet feeding. We aimed to clarify the efficacy of dietary intervention on the disease before and after the appearance of fibrosis. Male SHRSP5/Dmcr rats were divided into 9 groups; of these, 6 groups were fed control or HFC diet for several weeks and the remaining 3 groups represented the dietary intervention groups, which were fed the control diet after HFC diet feeding for 2 (before the appearance of fibrosis) or 8 (after the appearance of fibrosis) weeks. Dietary intervention before the appearance of fibrosis significantly improved the steatosis and reset the increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and serum total cholesterol (TC) levels. However, dietary intervention after the appearance of fibrosis was unable to reset the levels of hepatic TC, serum ALT, and fibrogenesis-related markers and had only a minor influence on hepatic fibrosis, although it reset the increased expression of transforming growth factor (TGF)-β1 and α-smooth muscle actin (SMA). It was noted that dietary intervention improved the increased AST levels; however, aggregated CD68-positive cells were still observed around the fibrosis area, which may be related to the findings of inflammatory cytokine mRNAs. Taken together, dietary intervention for fibrotic steatohepatitis improved steatosis, although it could not completely improve fibrosis.
authors.affiliation: Department of Human Life and Environment, Kinjo Gakuin University, Nagoya, Japan.
authors.forename: Hazuki
authors.initials: H
authors.lastname: Tamada
authors.affiliation: Department of Public Health, Fujita Health University School of Medicine, Nagoya, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Naito
authors.affiliation: Department of Human Life and Environment, Kinjo Gakuin University, Nagoya, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kitamori
authors.affiliation: Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Hayashi
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Yamagishi
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.affiliation: College of Life and Health Sciences, Chubu University, Kasugai, Japan.
authors.forename: Tamie
authors.initials: T
authors.lastname: Nakajima
mesh_terms: D000410:Alanine Transaminase / Q000096:biosynthesis; D000818:Animals; D015703:Antigens, CD / Q000097:blood; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001835:Body Weight; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D016207:Cytokines / Q000235:genetics; D004041:Dietary Fats / Q000008:administration & dosage* / Q000009:adverse effects; D018836:Inflammation Mediators / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D009929:Organ Size; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D000097382:CD68 Molecule
chemical_list: D000410:Alanine Transaminase; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001219:Aspartate Aminotransferases; D015415:Biomarkers; D002791:Cholesterol, Dietary; D016207:Cytokines; D004041:Dietary Fats; D018836:Inflammation Mediators; D008055:Lipids; D012333:RNA, Messenger; C539355:CD68 protein, rat; D000097382:CD68 Molecule