title: Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application.
abstract: An advanced optical biosensor was developed based on the enzymatic reaction with halogenated aliphatic hydrocarbons that is accompanied by the fluorescence change of pH indicator. The device is applicable for the detection of halogenated contaminants in water samples with pH ranging from 4 to 10 and temperature ranging from 5 to 60°C. Main advantages of the developed biosensor are small size (60×30×190mm(3)) and portability, which together with short measurement time of 1min belong to crucial attributes of analytical technique useful for routine environmental monitoring. The biosensor was successfully applied for the detection of several important halogenated pollutants under laboratory conditions, e.g., 1,2-dichloroethane, 1,2,3-trichloropropane and γ-hexachlorocyclohexane, with the limits of detection of 2.7, 1.4 and 12.1mgL(-1), respectively. The continuous monitoring was demonstrated by repetitive injection of halogenated compound into measurement solution. Consequently, field trials under environmental settings were performed. The presence of 1,2-dichloroethane (10mgL(-1)) was proved unambiguously on one of three potentially contaminated sites in Czech Republic, and the same contaminant was monitored on contaminated locality in Serbia. Equipped by Global Positioning System, the biosensor was used for creation of a precise map of contamination. Concentrations determined by biosensor and by gas chromatograph coupled with mass spectrometer exhibited the correlation coefficient of 0.92, providing a good confidence for the routine use of the biosensor system in both field screening and monitoring.
authors.affiliation: Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: bidmanova@sci.muni.cz.
authors.forename: Sarka
authors.initials: S
authors.lastname: Bidmanova
authors.affiliation: Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: KotlanovaM@seznam.cz.
authors.forename: Marketa
authors.initials: M
authors.lastname: Kotlanova
authors.affiliation: Global Change Research Centre, Academy of Sciences of the Czech Republic, Drasov 470, 664 24 Drasov, Czech Republic. Electronic address: rataj.t@czechglobe.cz.
authors.forename: Tomas
authors.initials: T
authors.lastname: Rataj
authors.affiliation: Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: jiri@chemi.muni.cz.
authors.forename: Jiri
authors.initials: J
authors.lastname: Damborsky
authors.affiliation: Photon Systems Instruments, spol. s r.o., Drasov 470, 664 24 Drasov, Czech Republic. Electronic address: martin@psi.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Trtilek
authors.affiliation: Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: zbynek@chemi.muni.cz.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Prokop
mesh_terms: D001419:Bacteria / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D005453:Fluorescence; D006843:Hydrocarbons, Chlorinated / Q000032:analysis* / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D006843:Hydrocarbons, Chlorinated; D014874:Water Pollutants, Chemical; D014867:Water; D006867:Hydrolases; C046137:haloalkane dehalogenase
keywords: Dehydrochlorinase; Environmental monitoring; Field-testing; Haloalkane dehalogenase; Halogenated pollutant; Optical biosensor