title: Micropropagation of Salvia wagneriana Polak and hairy root cultures with rosmarinic acid production.
abstract: Salvia wagneriana Polak is a tropical species native to Central America, well adapted to grow in the Mediterranean basin for garden decoration. Micropropagation has been assessed from axillary shoots of adult plants using a Murashige and Skoog basal medium, with the addition of 1.33-μM 6-benzylaminopurine for shoot proliferation; the subsequent rooting phase occurred in plant growth regulator-free medium. The plants were successfully acclimatised with high survival frequency. Hairy roots were induced after co-cultivation of leaf lamina and petiole fragments with Agrobacterium rhizogenes and confirmed by PCR. The establishment and proliferation of the selected HRD3 line were obtained in hormone-free liquid medium and the production of rosmarinic acid (RA) was evaluated after elicitation. The analysis of RA was performed by LC-ESI-DAD-MS in the hydroalcoholic extracts. The addition of casein hydrolysate increased the RA production, whereas no enrichment was observed after the elicitation with jasmonic acid.
authors.affiliation: a Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria CREA FSO- Unità di ricerca per la floricoltura e specie ornamentali-corso degli Inglesi , Sanremo , Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ruffoni
authors.affiliation: b Dipartimento di Farmacia , Università di Pisa , Pisa , Italy.|d Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute" , Università di Pisa , Pisa , Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bertoli
authors.affiliation: c Dipartimento di Scienze agrarie, alimentari e agro-ambientali , Università di Pisa , Pisa , Italy.|d Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute" , Università di Pisa , Pisa , Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Pistelli
authors.affiliation: b Dipartimento di Farmacia , Università di Pisa , Pisa , Italy.|d Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute" , Università di Pisa , Pisa , Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Pistelli
keywords: Agrobacterium rhizogenes; LC-ESI-DAD-MS; Salvia wagneriana Polak; casein hydrolysate; elicitation; rosmarinic acid