title: bFGF and VEGF improve the quality of vitrified-thawed human ovarian tissues after xenotransplantation to SCID mice.
abstract: OBJECTIVE
  The aim of this research is to study whether basic fibroblast growth factor (bFGF) alone or in combination with vascular endothelial growth factor (VEGF) could improve the quality of vitrified-thawed human ovarian tissue xenotransplanted to severe combined immune deficiency (SCID) mice.
  
  
  METHODS
  After collection and cryopreservation, thawed human ovarian tissue were cultured in vitro for 2 days and then xenografted to severe combined immune deficiency (SCID) mice for 7 days. The in vitro culture medium was separated into six groups, including (A) the blank control group, (B) the human recombinant bFGF (150 ng/ml) group, (C) the bFGF (150 ng/ml)+human recombinant VEGF (25 ng/ml) group, (D) bFGF (150 ng/ml)+VEGF (50 ng/ml) group, (E) bFGF (150 ng/ml)+ VEGF (75 ng/ml) group and (F) bFGF (150 ng/ml) + VEGF (100 ng/ml) group. In addition, eight pieces of thawed ovarian tissue were transplanted without in vitro culture, which serve as the fresh control group. The effect of transplantation was assessed by histological analysis, immunohistochemical staining for CD34, Ki-67, and AC-3 expression, and microvessel density (MVD).
  
  
  RESULTS
  There was no significant difference between the fresh and blank control group. Compared to the blank control group, the number of follicles, MVD, and rate of Ki-67-positive cells increased significantly in groups B, C, D, E, and F, while apoptosis decreased significantly. Compared to the bFGF treatment group, no significant difference appeared in group C, D, E, and F.
  
  
  CONCLUSIONS
  The administration of bFGF alone or in combination with VEGF improved the quality of postgraft human ovarian tissue, though VEGF, regardless of different concentrations, did not influence effect of bFGF.
authors.affiliation: Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Bei-Jia
authors.initials: BJ
authors.lastname: Kang
authors.affiliation: Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Zhun
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, Sichuan, 610041, People's Republic of China. lswart01@126.com.
authors.forename: Shang-Wei
authors.initials: SW
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D015925:Cryopreservation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000235:genetics; D064593:Heterografts; D006801:Humans; D051379:Mice; D055806:Microvessels / Q000187:drug effects / Q000254:growth & development; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000254:growth & development* / Q000637:transplantation; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage* / Q000235:genetics; D058989:Vitrification
chemical_list: C100666:KCB-1 protein, recombinant; D011994:Recombinant Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2
keywords: Angiogenesis; Human; Ovarian preservation; VEGF; Xenotransplantation; bFGF