title: Antibody- and Label-Free Phosphoprotein Sensor Device Based on an Organic Transistor.
abstract: An antibody- and label-free detection of a phosphoprotein (α-casein) has been achieved using an organic field-effect transistor (OFET)-based sensor. The fabricated OFET device possesses an extended-gate electrode functionalized with an artificial phosphoprotein receptor (Zn(II)-dipicolylamine complex, Zn(II)-DPA). It is shown that the OFET responds to the molecular recognition processes involving the Zn(II)-DPA at the extended-gate electrode. The binding of α-casein to the receptor in a HEPES solution results in an analyte-specific changes of the drain current of the OFET. The successful demonstration of the antibody- and label-free detection using the OFET could pave the way to the development of low-cost, disposable, and portable electronic sensor devices.
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University , 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Tsukuru
authors.initials: T
authors.lastname: Minamiki
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University , 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Minami
authors.affiliation: Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University , Bowling Green, Ohio 43403, United States.
authors.forename: Petr
authors.initials: P
authors.lastname: Koutnik
authors.affiliation: Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University , Bowling Green, Ohio 43403, United States.
authors.forename: Pavel
authors.initials: P
authors.lastname: Anzenbacher
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University , 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Tokito
mesh_terms: D000588:Amines / Q000737:chemistry; D000906:Antibodies; D002364:Caseins / Q000032:analysis* / Q000378:metabolism; D004566:Electrodes; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010848:Picolinic Acids / Q000737:chemistry; D014173:Transistors, Electronic; D015032:Zinc / Q000737:chemistry
chemical_list: C501847:2,2'-dipicolylamine; D000588:Amines; D000906:Antibodies; D002364:Caseins; D009942:Organometallic Compounds; D010848:Picolinic Acids; D015032:Zinc