title: Amentoflavone protects against psoriasis-like skin lesion through suppression of NF-κB-mediated inflammation and keratinocyte proliferation.
abstract: Psoriasis is a one of the most common chronic skin diseases, which affects 0.6-4.8% of the general population. Amentoflavone (AMF) belongs to the biflavonoid class of flavonoids, possessing various biological effects, such as anti-inflammatory, antioxidant, and anti-apoptotic effects. In the present study, we aimed to investigate the effect of AMF on psoriasis in imiquimod (IMQ) psoriasis-like lesions in mice and keratinocyte proliferation in HaCaT cells. We showed that AMF reduced skinfold thickening, and improved erythema and scaling scores and histological lesions in IMQ-treated mice. AMF exerted potent anti-inflammatory effect via influencing a variety of proinflammatory cytokines, including tumor necrosis factor α, interleukin (IL)-17A, IL-22, and IL-23 in local skin lesions and the whole body. In M5 (a cocktail of cytokines)-treated HaCaT cells, AMF significantly inhibited cell proliferation, promoted apoptosis, and inhibited the increase of expression of cyclin D1, cyclin E, IL-17A, and IL-22. In addition, AMF inhibited the upregulation of p65 NF-κB under psoriatic condition. Moreover, overexpression of p65 NF-κB significantly suppressed the effect of AMF on keratinocyte proliferation, apoptosis, and expression of cyclin D1, cyclin E, IL-17A, and IL-22. These results demonstrated that suppression of NF-κB was involved in AMF-resulted anti-proliferative, apoptosis-promoting, anti-inflammatory effects in keratinocytes. The data demonstrate that AMF may serve as potential therapeutic option for patients with psoriasis.
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710004, Shaanxi, People's Republic of China.
authors.forename: Jingang
authors.initials: J
authors.lastname: An
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710004, Shaanxi, People's Republic of China.
authors.forename: Zhengxiao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710004, Shaanxi, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710004, Shaanxi, People's Republic of China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710004, Shaanxi, People's Republic of China. jia_huo555@126.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Huo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D044946:Biflavonoids / Q000008:administration & dosage* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000494:pharmacology*; D011565:Psoriasis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D012885:Skinfold Thickness
chemical_list: D000893:Anti-Inflammatory Agents; D044946:Biflavonoids; D016207:Cytokines; D016328:NF-kappa B; C011164:amentoflavone
keywords: Amentoflavone; Inflammation; Keratinocyte proliferation; NF-κB; Psoriasis