title: Comparisons of Ribosomal Protein Gene Promoters Indicate Superiority of Heterologous Regulatory Sequences for Expressing Transgenes in Phytophthora infestans.
abstract: Molecular genetics approaches in Phytophthora research can be hampered by the limited number of known constitutive promoters for expressing transgenes and the instability of transgene activity. We have therefore characterized genes encoding the cytoplasmic ribosomal proteins of Phytophthora and studied their suitability for expressing transgenes in P. infestans. Phytophthora spp. encode a standard complement of 79 cytoplasmic ribosomal proteins. Several genes are duplicated, and two appear to be pseudogenes. Half of the genes are expressed at similar levels during all stages of asexual development, and we discovered that the majority share a novel promoter motif named the PhRiboBox. This sequence is enriched in genes associated with transcription, translation, and DNA replication, including tRNA and rRNA biogenesis. Promoters from the three P. infestans genes encoding ribosomal proteins S9, L10, and L23 and their orthologs from P. capsici were tested for their ability to drive transgenes in stable transformants of P. infestans. Five of the six promoters yielded strong expression of a GUS reporter, but the stability of expression was higher using the P. capsici promoters. With the RPS9 and RPL10 promoters of P. infestans, about half of transformants stopped making GUS over two years of culture, while their P. capsici orthologs conferred stable expression. Since cross-talk between native and transgene loci may trigger gene silencing, we encourage the use of heterologous promoters in transformation studies.
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California, United States of America.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Poidevin
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California, United States of America.
authors.forename: Kalina
authors.initials: K
authors.lastname: Andreeva
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California, United States of America.
authors.forename: Careen
authors.initials: C
authors.lastname: Khachatoorian
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California, United States of America.
authors.forename: Howard S
authors.initials: HS
authors.lastname: Judelson
mesh_terms: D020868:Gene Silencing / Q000502:physiology; D017930:Genes, Reporter / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D055750:Phytophthora infestans / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics*; D000080882:Ribosomal Protein L10; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics*
chemical_list: D038621:Regulatory Sequences, Ribonucleic Acid; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D012343:RNA, Transfer