title: Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.
abstract: Oleanolic acid (OA) and its several derivatives possess chemopreventive and chemotherapeutic functions against a series of cancer types. Many chemotherapeutic compounds are effective in improving the quality of life and prolonging the survival of patients with gastric cancer, therefore progress in the treatment of gastric cancer, especially the anticancer effects of OA derivatives must be achieved. The inhibitory effect of SZC017, a newly synthesized derivative of OA, on cell viability was determined by MTT assay. Furthermore, flow cytometry, transmission electron microscopy, and western blot analysis revealed that the inhibition of cell viability by OA was mediated by triggering the intrinsic apoptosis of gastric cancer cells, and inducing S phase arrest of SGC7901 cells. Mechanistically, SZC017 was effective against gastric cancer cells via inhibiting Akt/NF‑κB signaling and topoisomerase I and IIα proteins. Taken together, our data indicate that SZC017 may be a potential chemotherapeutic agent against gastric cancer cells.
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, Liaoning 116024, P.R. China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Bining
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Guozhu
authors.initials: G
authors.lastname: Han
authors.affiliation: College of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, Liaoning 116024, P.R. China.
authors.forename: Shisheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Zeyao
authors.initials: Z
authors.lastname: Tang
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis / Q000235:genetics; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020672:I-kappa B Proteins / Q000096:biosynthesis / Q000235:genetics; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D010880:Piperidines / Q000138:chemical synthesis / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology*; D059003:Topoisomerase Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D009363:Neoplasm Proteins; D010880:Piperidines; C000611552:SZC017; D059003:Topoisomerase Inhibitors; D000072000:NF-KappaB Inhibitor alpha; D009828:Oleanolic Acid; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt; D004264:DNA Topoisomerases, Type I