title: Effect of apolipoprotein B mRNA-editing catalytic polypeptide-like protein-3G in cervical cancer.
abstract: Cervical cancer is one of the most common gynecologic cancers. The role of apolipoprotein B mRNA-editing catalytic polypeptide-like protein-3G (APCBEC-3G) in cervical cancer has yet to be elucidated. This study intends to explore the effect of APCBEC-3G on cervical cancer cell proliferation and invasion. In vitro, the cervical cancer cell line Hela was transfected by APCBEC-3G plasmid. The mRNA and protein expression levels of APCBEC-3G were detected by Real-time PCR and Western blot, respectively. Cervical cancer cell proliferation was determined by MTT. Transwell assay was applied to measure the effect of APCBEC-3G on cell invasion. APCBEC-3G mRNA and protein increased significantly after transfection (P<0.05) and cervical cancer cell proliferation and invasive ability were decreased significantly (P<0.05). APOBEC-3G serves as a suppressor of cervical cancer cell proliferation and invasion. Our research provides theoretical basis for further investigation APOBEC-3G effect in cervical cancer occurrence and development.
authors.affiliation: Department of Gynecology, Jinan Maternity and Child Health Care Hospital Jinan 250001, Shandong, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Gynecology Ultrasound, Jinan Maternity and Child Health Care Hospital Jinan 250001, Shandong, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Leng
authors.affiliation: Department of Gynecology, Jinan Maternity and Child Health Care Hospital Jinan 250001, Shandong, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xue
authors.affiliation: Department of Pharmacy, Jinan Maternity and Child Health Care Hospital Jinan 250001, Shandong, China.
authors.forename: Ruiqian
authors.initials: R
authors.lastname: Dong
mesh_terms: D000071479:APOBEC-1 Deaminase / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D000071479:APOBEC-1 Deaminase
keywords: APOBEC-3G; cervical cancer; invasion; proliferation