title: Sequencing and analysis of the complete mitochondrial genome of Hyla ussuriensis (Anura: Hylidae).
abstract: In this study, the complete mitogenome sequence of Hyla ussuriensis (Anura: Hylidae) is first determined using long PCR. It is a circular molecule of 18 023 bp in length (GenBank accession no. KT964710). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of Hyla ussuriensis contained two rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs), and a control region (D-loop). The nucleotide composition was 29.9% A, 25.4% C, 14.5% G, and 30.2% T. Mitochondrial genomes analyses based on NJ method yield phylogenetic trees, indicating 13 reported Anura frogs belonging to five families (Hylidae, Bufonidae, Microhylidae, Ranidae, and Rhacophoridae). These molecular data presented here provide a useful tool for systematic analyses of genus Hyla and family Hylidae.
authors.affiliation: a College of Life Science and Technology, Harbin Normal University , Harbin , Heilongjiang , PR China.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Sun
authors.affiliation: a College of Life Science and Technology, Harbin Normal University , Harbin , Heilongjiang , PR China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Xie
authors.affiliation: a College of Life Science and Technology, Harbin Normal University , Harbin , Heilongjiang , PR China.
authors.forename: Wenge
authors.initials: W
authors.lastname: Zhao
authors.affiliation: a College of Life Science and Technology, Harbin Normal University , Harbin , Heilongjiang , PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D050259:Genes, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C075526:RNA, ribosomal, 12S
keywords: Hyla ussuriensis; Hylidae; mitogenome; phylogenetic tree