title: Orthotopic Injection of Pancreatic Cancer Cells.
abstract: Pancreatic ductal adenocarcinoma is an aggressive disease with a 5-yr survival rate of only 5%. The location of the pancreas in the abdomen, where it is obscured by other organs, makes it a difficult tissue to study and manipulate. This protocol describes in detail how to orthotopically inject cancer cells into the pancreas in mice. This technique is particularly useful when the cells must be manipulated in ways that cannot be modeled genetically.
authors.affiliation: Department of Gastroenterology and Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Aiello
authors.affiliation: Department of Gastroenterology and Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Rhim
authors.affiliation: Department of Gastroenterology and Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
authors.forename: Ben Z
authors.initials: BZ
authors.lastname: Stanger
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D004847:Epithelial Cells / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000473:pathology*