title: Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes.
abstract: Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased toward those that can be stably produced in commonly used mesophilic host systems. Two main strategies are currently being explored for the development of low-temperature expression in bacterial hosts: (i) low-temperature adaption of existing mesophilic expression systems, and (ii) development of new psychrophilic hosts. These developments include genetic engineering of the expression cassettes to optimize the promoter/operator systems that regulate heterologous expression. In this addendum we present our efforts in the development of such low-temperature expression systems, and speculate about future advancements in the field and potential applications.
authors.affiliation: a University of Tromsø, Norstruct, Department of Chemistry, Faculty of Science and Technology , Tromsø , Norway.
authors.forename: Gro Elin Kjæreng
authors.initials: GE
authors.lastname: Bjerga
authors.affiliation: b Norwegian University of Science and Technology , Department of Biotechnology , Trondheim , Norway.
authors.forename: Rahmi
authors.initials: R
authors.lastname: Lale
authors.affiliation: a University of Tromsø, Norstruct, Department of Chemistry, Faculty of Science and Technology , Tromsø , Norway.
authors.forename: Adele Kim
authors.initials: AK
authors.lastname: Williamson
mesh_terms: D001110:Arctic Regions; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D003080:Cold Temperature; D015964:Gene Expression Regulation, Bacterial; D007218:Industrial Microbiology; D060847:Metabolic Engineering / Q000379:methods; D055550:Protein Stability; D031161:Pseudoalteromonas / Q000235:genetics* / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins
keywords: AraC/PBAD; Heterologous expression; Pseudomonas; T7 RNA polymerase; T7 promoter; XylS/Pm; cold-adapted; cspA promoter; psychrophilic