title: MiR-130b inhibits proliferation and induces apoptosis of gastric cancer cells via CYLD.
abstract: A role of microRNA-130b (miR-130b) in the carcinogenesis of gastric cancer remains undetermined. In this study, we studied the effects and mechanism of miR-130b to the gastric cell proliferation and apoptosis. We found that the levels of miR-130b significantly up-regulated in gastric cancer tissue, compared to the paired adjacent non-tumor gastric tissue. The miR-130b levels in gastric cancer cell lines were significantly higher than those in control normal gastric tissues. Transfection with the miR-130b mimic enhanced the cell proliferation and suppressed cell apoptosis in gastric cancer cells, while transfection with the anti-sense of miR-130b (anti-miR-130b) suppressed cell proliferation and induced cell apoptosis in gastric cancer cells. Bioinformatics analyses showed that cylindromatosis gene (CYLD) was a potential target gene of miR-130b. The luciferase activity assay and western blot verified that miR-130b targeted CYLD messenger RNA (mRNA) to modulate its protein levels. Together, our study suggests that aberrantly expressed miR-130b may regulate cell apoptosis and proliferation of human gastric cancer cells via CYLD, which appears to be a promising therapeutic target for gastric cancer.
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250014, China.
authors.forename: Baoyou
authors.initials: B
authors.lastname: Sun
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250014, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250014, China.
authors.forename: Wendong
authors.initials: W
authors.lastname: Ma
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250014, China.
authors.forename: Shikang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Huadong Hospital of Fudan University, 221 Yananxi Road, Shanghai, 200040, China. chunjin_huang@163.com.
authors.forename: Chunjin
authors.initials: C
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D000074749:Deubiquitinating Enzyme CYLD; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; C409984:CYLD protein, human; D000074749:Deubiquitinating Enzyme CYLD
keywords: Apoptosis; CYLD; Gastric cancer; Proliferation; miR-130b