title: [Establishment of Humanized Mouse Model by Using Transplantation of Mobilized Peripheral Blood Stem Cells].
abstract: OBJECTIVE
  To investigate the hematopoietic reconstitution in immunodeficiency NPG(TM) mice after transplantation of G-CSF-mobilized peripheral blood CD34(+) hemopoietic stem cells.
  
  
  METHODS
  CD34(+) cells were isolated from peripheral blood stem cells (PBSC) by magnetic activated cell sorting (MACS), and then were transplanted into NPG(TM) mice irradiated with sublethal dose of X ray by marrow cavity transplantation. The hemogram of mice after transplantation for 2, 4 weeks was observed; human cell populations (CD45(+), CD19(+)) in the peripheral blood of mice were dynamically analyzed by flow cytometry (FCM) at 4, 6, 8, 10 and 12 weeks after transplantation. Until the planned harvest at the 12 week after transplantation, the CD45(+), CD19(+) level in bone marrow, liver, spleen from each mouse were detected by flow cytometry; the expression of human Alu gene in the bone marrow cell of mouse was detected by PCR.
  
  
  RESULTS
  The purity of CD34(+) cells accounted for 96.3%; after irradiation, the nucleated cells and megalokaryocytes in the marrow cavity of NPG mice were reduced significantly or were lost, and reached the myeloablative effect. At week 4 after transplantation, components of blood cells in peripheral blood of transplanted mice were recovered to the level before irradiation; all the mice survived, human CD45(+), CD19(+) cells were found by FCM in the peripheral blood of all the surviving mice in transplantation group at week 4, 6, 8, 10, 12 after the transplantation; at the 12th week, the human Alu gene could be detected in the bone marrow of all the mice in transplantation group.
  
  
  CONCLUSIONS
  The human-mouse chimeric model is successfully established in irradiation-induced NPG mouse by transplantation of CD34(+) HSC from G-CSF-mobilized peripheral blood via marrow cavity.
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.|Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Xu
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.|Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. E-mail: chenliyhhd@sina.com.
authors.forename: Yu-Hang
authors.initials: YH
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratial Examination, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Chen
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.|Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Bing-Han
authors.initials: BH
authors.lastname: Zou
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.|Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.affiliation: Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Kong
authors.affiliation: Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Sheng
authors.affiliation: Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Hu
authors.affiliation: Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.|Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. E-mail: zb307ctc@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.|Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. E-mail: huliangding@sohu.com.
authors.forename: Liang-Ding
authors.initials: LD
authors.lastname: Hu
mesh_terms: D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D036101:Cord Blood Stem Cell Transplantation; D004195:Disease Models, Animal; D016179:Granulocyte Colony-Stimulating Factor; D006412:Hematopoietic Stem Cells; D006801:Humans; D051379:Mice; D013154:Spleen
chemical_list: D016179:Granulocyte Colony-Stimulating Factor