title: Lateral ventricle injection of orexin-A ameliorates central precocious puberty in rat via inhibiting the expression of MEG3.
abstract: BACKGROUND
  Central precocious puberty (CPP) is characterized as increasing gonadotropin-releasing hormone (GnRH) release. Orexin-A has also been shown to affect GnRH release. However, there are few reports about the effect of orexin A on the treatment of CPP.
  
  
  METHODS
  After establishing the precocious puberty model, the rats were divided into four groups: normal control, precocious puberty rats, precocious puberty rats treated with normal saline and precocious puberty rats treated with orexin-A. The vaginal opening time, second estrus cycle, ovarian index and uterus index of rats in each group were detected. qRT-PCR was performed to examine the expression of MEG3 and kisspeptin in rats. HT22 cells were transfected with pcDNA-MEG3 to detect the expression of Kisspeptin.
  
  
  RESULTS
  In this study, we found that orexin-A not only delayed the day of vaginal opening and regular estrus cycle days but also decreased the ovarian index and uterus index in rats with CPP. In addition, orexin-A reversed the up-regulation of MEG3 and kisspeptin in rats with CPP. In HT22 cells, the mRNA and protein level of kisspeptin were enhanced by pcDNA-MEG3.
  
  
  CONCLUSIONS
  Our results suggest that orexin-A ameliorates central precocious puberty in rat and MEG3 might be involved in this effect, suggesting that MEG3 might be a novel target in treating central precocious puberty.
authors.affiliation: Department of Pediatrics, Northern Jiangsu People's Hospital Yangzhou 225001, China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Tao
authors.affiliation: Department of Pediatrics, Northern Jiangsu People's Hospital Yangzhou 225001, China.
authors.forename: Nazir
authors.initials: N
authors.lastname: Sharif
authors.affiliation: Department of Pediatrics, Northern Jiangsu People's Hospital Yangzhou 225001, China.
authors.forename: Bi-He
authors.initials: BH
authors.lastname: Zeng
authors.affiliation: Department of Pediatrics, Northern Jiangsu People's Hospital Yangzhou 225001, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Cai
authors.affiliation: Department of Pediatrics, Northern Jiangsu People's Hospital Yangzhou 225001, China.
authors.forename: Yu-Xiu
authors.initials: YX
authors.lastname: Guo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D004195:Disease Models, Animal; D005260:Female; D007276:Injections, Intraventricular; D059648:Kisspeptins / Q000096:biosynthesis; D020547:Lateral Ventricles; D051379:Mice; D000068797:Orexins / Q000008:administration & dosage*; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012741:Sexual Maturation / Q000187:drug effects* / Q000502:physiology*; D014162:Transfection
chemical_list: C491406:Kiss1 protein, rat; D059648:Kisspeptins; D000068797:Orexins; D062085:RNA, Long Noncoding
keywords: MEG3; Orexin-A; central precocious puberty; hypothalamic-pituitary-gonadal (HPG) axis; kisspeptin