title: Sydnone 1: A Mesoionic Compound with Antitumoral and Haematological Effects In Vivo.
abstract: This study evaluated the antitumour activity of the mesoionic compound sydnone 1 (Syd-1) against Walker-256 carcinosarcoma. Tumour cells were subcutaneously inoculated in the hind limb in male Wistar rats. The animals were orally treated for 12 days with Syd-1 (75 mg/kg) or vehicle. At the end of treatment, considerable decreases in tumour volume and tumour weight were observed in treated animals. Samples of these tumours presented increases in apoptotic bodies and pro-apoptotic protein expression (Bax and p53), while the expression of the anti-apoptotic protein Bcl-2 was reduced. A decrease in reduced glutathione levels and an increase in glutathione peroxidase activity were observed in tumour after Syd-1 treatment. However, significant splenomegaly was evident in animals that received Syd-1, most likely attributable to the induction of haemolysis. This study demonstrated the antitumour activity of Syd-1 against Walker-256 carcinosarcoma. Its mechanism of action is linked to the activation of apoptotic pathways that lead to tumour cell death.
authors.affiliation: Department of Pharmacology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Larissa Favaretto
authors.initials: LF
authors.lastname: Galuppo
authors.affiliation: Department of Pharmacology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Francislaine Aparecida
authors.initials: FA
authors.lastname: Dos Reis Lívero
authors.affiliation: Department of Pharmacology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Gracianny Gomes
authors.initials: GG
authors.lastname: Martins
authors.affiliation: Department of Pharmacology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Cibele Campos
authors.initials: CC
authors.lastname: Cardoso
authors.affiliation: Department of Veterinary Medicine, Federal University of Parana, Curitiba, Brazil.
authors.forename: Olair Carlos
authors.initials: OC
authors.lastname: Beltrame
authors.affiliation: Department of Basic Pathology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Liliane Maria Bacaro
authors.initials: LM
authors.lastname: Klassen
authors.affiliation: Department of Chemistry, Federal Rural University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: André Vinicius Dos Santos
authors.initials: AV
authors.lastname: Canuto
authors.affiliation: Department of Chemistry, Federal Rural University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Aurea
authors.initials: A
authors.lastname: Echevarria
authors.affiliation: Department of Medical Pathology, Federal University of Parana, Curitiba, Brazil.
authors.forename: José Ederaldo Queiroz
authors.initials: JE
authors.lastname: Telles
authors.affiliation: Department of Basic Pathology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Giseli
authors.initials: G
authors.lastname: Klassen
authors.affiliation: Department of Pharmacology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Acco
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002279:Carcinoma 256, Walker / Q000188:drug therapy*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013558:Sydnones / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C494828:Bax protein, rat; D013558:Sydnones; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; C505410:Casp3 protein, rat; D053148:Caspase 3; D005978:Glutathione