title: Lymphocytes with multiple chromosomal damages in a large cohort of West Siberia residents: Results of long-term monitoring.
abstract: Cells with specific multiple chromosome aberrations, defined as rogue cells (RC) have been described in different populations, predominantly those exposed to radiation. The frequency, etiology and related health risks have still not been elucidated due to their low frequency of occurrences and rarely performed studies. This study reports RC frequency using chromosome aberration (CA) assay in peripheral lymphocytes in the group of 3242 subjects, during a 30-year long follow-up study in a general rural and urban population, children environmentally exposed to radon, occupationally exposed population and lung cancer patients from the Kemerovo region (Siberia, Russian Federation). Results show that the highest RC frequency was present in children environmentally exposed to radon and the lowest in the general urban population. Total frequency of CA did not correlate with frequency of RC. Genotoxic analysis of air and water samples excluded anthropogenic pollution as a possible cause of genome damage and RC frequency. In 85% of RCs, double minutes, observed in a large number of human tumors, were present. Results of CA analysis suggested that radon and its decay products (alpha-emitters) were the leading factors causing RC in subjects exposed to high LET radiation. Thus, RC may be a candidate biomarker for exposure to this type of radiation.
authors.affiliation: Kemerovo State University, Kemerovo, Russia; Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Kemerovo, Russian Federation.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Druzhinin
authors.affiliation: Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Kemerovo, Russian Federation.
authors.forename: Maria
authors.initials: M
authors.lastname: Bakanova
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia. Electronic address: afucic@imi.hr.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Fucic
authors.affiliation: Kemerovo State University, Kemerovo, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Golovina
authors.affiliation: Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Kemerovo, Russian Federation.
authors.forename: Yana
authors.initials: Y
authors.lastname: Savchenko
authors.affiliation: Kemerovo State University, Kemerovo, Russia.
authors.forename: Maxim
authors.initials: M
authors.lastname: Sinitsky
authors.affiliation: Kemerovo State University, Kemerovo, Russia.
authors.forename: Valentin
authors.initials: V
authors.lastname: Volobaev
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002869:Chromosome Aberrations; D015331:Cohort Studies; D004249:DNA Damage; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D018499:Linear Energy Transfer; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects; D011886:Radon / Q000633:toxicity; D012424:Rural Population; D012800:Siberia; D014505:Urban Population; D055815:Young Adult
chemical_list: D011886:Radon
keywords: Chromosome aberration; Double minute; High LET radiation; In vivo irradiation; Lymphocyte; Radon; Rogue cell