title: Altered microRNA Expression and Immunosuppressive Cytokine Production by Regulatory T Cells of Ulcerative Colitis Patients.
abstract: Regulatory T (Treg) cells are essential for maintenance of peripheral tolerance and prevention of autoimmune diseases in part by producing immunosuppressive cytokines. Recently, microRNAs (miRNAs) have also been involved in autoimmune disorders, not least for their crucial role in the regulation of Treg biology and function. We simultaneously investigated the concentration of IL-35, IL-10, TGF-β, and sCD25 in supernatant of cell culture and the expression patterns of several miRNAs in CD4(+)CD25(+) CD127(-/low) FoxP3(+) Tregs of ulcerative colitis (UC) patients. Significantly lower levels of IL-10 and IL-35 were observed in Treg cultures of UC patients. miR-21, miR-146a, and miR-155 levels were downregulated and miR-31 level was upregulated in Tregs of patients. Our results suggest that microRNAs may serve as a novel regulator in function and homoeostasis of UC Treg cells, providing a key role for them in pathophysiology of UC.
authors.affiliation: a Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Mousa
authors.initials: M
authors.lastname: Mohammadnia-Afrouzi
authors.affiliation: a Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Ahmad Zavaran
authors.initials: AZ
authors.lastname: Hosseini
authors.affiliation: b Department of Immunology , Mazandaran University of Medical Sciences , Sari , Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khalili
authors.affiliation: c Immunogenetics Research Center, Mazandaran University of Medical Sciences , Sari , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Abediankenari
authors.affiliation: d Department of Immunology, Faculty of Medicine , Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Amari
authors.affiliation: e Department of Immunology , School of Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Babak
authors.initials: B
authors.lastname: Aghili
authors.affiliation: b Department of Immunology , Mazandaran University of Medical Sciences , Sari , Iran.
authors.forename: Hadi Hossein
authors.initials: HH
authors.lastname: Nataj
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers; D016022:Case-Control Studies; D016000:Cluster Analysis; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D056747:Immunomodulation; D016130:Immunophenotyping; D018655:Lymphocyte Count; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D005810:Multigene Family; D012720:Severity of Illness Index; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D016207:Cytokines; D035683:MicroRNAs
keywords: IL-10; IL-35; microRNA; regulatory T cells; ulcerative colitis