title: miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition.
abstract: Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significantly increased in cervical cancer cells and tissues, and upregulation of miR-27b was negatively associated with its direct target, cadherin 11 (CDH11). Upregulation of miR-27b significantly accelerated the proliferation, cell cycle transition from G1 to S phase, migration and invasion of C33A cells, while downregulation of miR-27b suppressed the proliferation and invasion of HeLa cells. Moreover, CDH11 cDNA transfection impaired the oncogenic effect of miR-27b on cancer cells. Knockdown of CDH11 attenuated the suppressive effect of an miR-27b inhibitor on cervical cancer cells. In addition, we found that CDH11 was involved in miR-27b-induced epithelial-mesenchymal transition (EMT) by regulating expression of E-cadherin, vimentin and N-cadherin. Our results for the first time indicate that miR-27b acting as an oncogene may play an important role in the progression of cervical cancer by modulating CDH11 and EMT.
authors.affiliation: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Jihang
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Boya
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Le
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dou
authors.affiliation: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Guo
mesh_terms: D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D015820:Cadherins; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; C086960:osteoblast cadherin