title: Screening of new antileukemic agents from essential oils of algae extracts and computational modeling of their interactions with intracellular signaling nodes.
abstract: Microalgae are very rich in bioactive compounds, minerals, polysaccharides, poly-unsaturated fatty acids and vitamins, and these rich constituents make microalgae an important resource for the discovery of new bioactive compounds with applications in biotechnology. In this study, we studied the antileukemic activity of several chosen microalgae species at the molecular level and assessed their potential for drug development. Here we identified Stichococcus bacillaris, Phaeodactylum tricornutum, Microcystis aeruginosa and Nannochloropsis oculata microalgae extracts with possible antileukemic agent potentials. Specifically we studied the effects of these extracts on intracellular signal nodes and apoptotic pathways. We characterized the composition of essential oils of these fifteen different algae extracts using gas chromatography-mass spectrometry (GC-MS). Finally, to identify potential molecular targets causing the phenotypic changes in leukemic cell lines, we docked a selected group of these essential oils to several key intracellular proteins. According to results of rank score algorithm, five of these essential oils analyzed might be considered as in silico plausible candidates to be used as antileukemic agents.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey. Electronic address: belkisatasever.arslan@uskudar.edu.tr.
authors.forename: Belkis
authors.initials: B
authors.lastname: Atasever-Arslan
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey.
authors.forename: Kaan
authors.initials: K
authors.lastname: Yilancioglu
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey; Neuroscience Program, Health Sciences Institute, Uskudar University, Istanbul, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Kalkan
authors.affiliation: Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey.
authors.forename: Ahmet Can
authors.initials: AC
authors.lastname: Timucin
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey.
authors.forename: Hazal
authors.initials: H
authors.lastname: Gür
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey.
authors.forename: Fatma Busra
authors.initials: FB
authors.lastname: Isik
authors.affiliation: Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey; Sabanci University Nanotechnology Research and Application Center-SUNUM, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Deniz
authors.affiliation: Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey; Sabanci University Nanotechnology Research and Application Center-SUNUM, Turkey.
authors.forename: Batu
authors.initials: B
authors.lastname: Erman
authors.affiliation: Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Cetiner
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D006801:Humans; D007938:Leukemia; D058086:Microalgae; D008954:Models, Biological; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011485:Protein Binding
chemical_list: D000970:Antineoplastic Agents; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Algae; Essential oils; GC–MS; Leukemia