title: In vivo wound healing activity of gels containing Cecropia pachystachya leaves.
abstract: OBJECTIVE
  To evaluate the in-vivo wound healing and in-vitro antioxidant activity of gels containing the ethyl acetate extract of Cecropia pachystachya (ECP) 2% and 5%, and to perform the chemical fingerprint of ECP by HPLC-DAD.
  
  
  METHODS
  The wound healing activity of the gels was evaluated for 21 days, using the excision model in rats followed by histopathological and histomorphometric analysis. The in-vitro antioxidant effect of ECP was investigated using 1-diphenyl-2-picrylhydrazyl, reducing power, β-carotene bleaching and thiobarbituric acid reactive substances assays. Also, a HPLC analysis was performed to identify the chemical markers orientin, iso-orientin and chlorogenic acid.
  
  
  RESULTS
  The group of animals treated with ECP 5% presented oedema and inflammatory infiltrate with less intensity than the other groups. Both ECP 2% and 5% gels showed less neovascularization and cellularity, and better tissue repair when compared to the control, which showed a younger and homogeneous tissue.
  
  
  CONCLUSIONS
  This study had demonstrated that the ECP gels promoted the acceleration of the healing process when compared to the control group. Wound contraction, angiogenesis, epithelialization and the collagen deposition support further evaluation of C. pachystachya leaves in the topical treatment and management of skin wounds.
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Ana Paula do Nascimento
authors.initials: AP
authors.lastname: Duque
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Nícolas de Castro Campos
authors.initials: Nde C
authors.lastname: Pinto
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Renata de Freitas
authors.initials: Rde F
authors.lastname: Mendes
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Josiane Mello
authors.initials: JM
authors.lastname: da Silva
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Danielle Maria de Oliveira
authors.initials: DM
authors.lastname: Aragão
authors.affiliation: Department of Morphology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Maria Christina Marques Nogueira
authors.initials: MC
authors.lastname: Castañon
authors.affiliation: Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Elita
authors.initials: E
authors.lastname: Scio
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D035702:Cecropia Plant / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D005782:Gels / Q000737:chemistry* / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002726:Chlorogenic Acid; D005419:Flavonoids; D005782:Gels; D005960:Glucosides; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C010574:biphenyl; C065886:orientin
keywords: Cecropia pachystachya; antioxidant; phenolic compounds; wound healing