title: Developmental and hormone-induced changes of mitochondrial electron transport chain enzyme activities during the last instar larval development of maize stem borer, Chilo partellus (Lepidoptera: Crambidae).
abstract: The energy demand for structural remodelling in holometabolous insects is met by cellular mitochondria. Developmental and hormone-induced changes in the mitochondrial respiratory activity during insect metamorphosis are not well documented. The present study investigates activities of enzymes of mitochondrial electron transport chain (ETC) namely, NADH:ubiquinone oxidoreductase or complex I, Succinate: ubiquinone oxidoreductase or complex II, Ubiquinol:ferricytochrome c oxidoreductase or complex III, cytochrome c oxidase or complex IV and F1F0ATPase (ATPase), during Chilo partellus development. Further, the effect of juvenile hormone (JH) analog, methoprene, and brain and corpora-allata-corpora-cardiaca (CC-CA) homogenates that represent neurohormones, on the ETC enzyme activities was monitored. The enzymatic activities increased from penultimate to last larval stage and thereafter declined during pupal development with an exception of ATPase which showed high enzyme activity during last larval and pupal stages compared to the penultimate stage. JH analog, methoprene differentially modulated ETC enzyme activities. It stimulated complex I and IV enzyme activities, but did not alter the activities of complex II, III and ATPase. On the other hand, brain homogenate declined the ATPase activity while the injected CC-CA homogenate stimulated complex I and IV enzyme activities. Cumulatively, the present study is the first to show that mitochondrial ETC enzyme system is under hormone control, particularly of JH and neurohormones during insect development.
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: V
authors.initials: V
authors.lastname: VenkatRao
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Chaitanya
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: D
authors.initials: D
authors.lastname: Naresh Kumar
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: M
authors.initials: M
authors.lastname: Bramhaiah
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: apdgsl@uohyd.ernet.in.
authors.forename: A
authors.initials: A
authors.lastname: Dutta-Gupta
mesh_terms: D000818:Animals; D003335:Corpora Allata / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007605:Juvenile Hormones / Q000378:metabolism / Q000494:pharmacology; D007814:Larva; D007915:Lepidoptera / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D008675:Metamorphosis, Biological / Q000187:drug effects; D008726:Methoprene / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D011679:Pupa / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D003313:Zea mays / Q000469:parasitology*
chemical_list: D007605:Juvenile Hormones; D024101:Mitochondrial Proteins; D008726:Methoprene
keywords: Corpora allata; Corpora cardiaca; Electron transport chain; Insect brain; Juvenile hormone; Mitochondria; Neurohormone