title: [Construction of host-vector balanced lethal system of Salmonella typhimurium SL1344Δcya mutant and immune protection test of chickling].
abstract: OBJECTIVE
  To develop a host-vector balanced lethal system of Salmonella typhimurium adenylate cyclase mutant, and detect its biological characteristics.
  
  
  METHODS
  We constructed SL1344ΔcyaΔasd mutant strain by recombinant suicide plasmid (pREAasd), and screened by two-step method, transformed pYA3493 plasmid containing the asd gene without resistance electric into the lack of SL1344 AcyaΔasd, then the recombinant strains SL1344 ΔcyaΔasd (pYA3493) was constructed successfully.
  
  
  RESULTS
  The biochemical characteristics and growth rate of the mutant were different from that of the wild strain SL1344, but almost the same as that of the parent strain SL1344Δcya. The mutant strain could neither ferment maltose, lactose, and sorbitol, nor decompose H2S, galactose and rat lee sugar, but still retained the ability to use glucose. The one-day chicken lethal test showed that SL1344ΔcyaΔasd (pYA3493) mutant was at least 104 times lower than SL1344 strain. The protection rate induced by the SL1344ΔcyaΔasd (pYA3493) mutant was 62. 5%.
  
  
  CONCLUSIONS
  The SL1344ΔcyaΔasd (pYA3493) mutant was successfully constructed, and had good immune protection, it laid a foundation for developing potential oral vaccines.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Songbiao
authors.initials: S
authors.lastname: Chen
authors.forename: Zuhua
authors.initials: Z
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.forename: Xiaokang
authors.initials: X
authors.lastname: Li
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jia
authors.forename: Chuan
authors.initials: C
authors.lastname: Yu
authors.forename: Chunjie
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiangchao
authors.initials: X
authors.lastname: Cheng
authors.forename: Yinju
authors.initials: Y
authors.lastname: Li
authors.forename: Tingcai
authors.initials: T
authors.lastname: Wu
authors.forename: Zhanqin
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D002645:Chickens; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D008297:Male; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated