title: Survival of Cochlear Spiral Ganglion Neurons Improved In vivo by Anti-miR204 via TMPRSS3.
abstract: OBJECTIVE
  Sensorineural hearing loss (SNHL) is caused by damage to hair cells followed by degeneration of the spiral ganglion neurons (SGNs), and cochlear implanting is an effective treatment. Unfortunately, the progressive hearing loss is still found due to ongoing degeneration of cochlear SGNs. The aim of this study was to investigate the neuroprotective effect of anti-miR204 on SGNsin vivo.
  
  
  METHODS
  Our recentin vitrowork suggested that anti-miR204 could be a potential therapeutic strategy in SNHLviarescue cochlear SGNs. In order to further our knowledge of miR204 on SGNsin vivo, we made a kanamycin ototoxicity model and then virus containing the anti-miR204 gene (AAV1-anti-miR204) was microinjected into the cochlear of the model to monitor the effect.
  
  
  RESULTS
  The SGNs were rescued by anti-miR204 in the kanamycin ototoxicity mouse group compared to the sham group. Moreover, expression of TMPRSS3 in SGNs was saved by anti-miR204 treatment.
  
  
  CONCLUSIONS
  Anti-miR204 might be an alternate way to alleviate the degeneration of cochlear SGNs of kanamycin ototoxicity mice.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: A
authors.initials: A
authors.lastname: Peng
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: X
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: S
authors.initials: S
authors.lastname: Ge
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China.
authors.forename: G
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital of Central South University, Hunan, China, e-mail: jiajialiu2014@163.com.
authors.forename: J
authors.initials: J
authors.lastname: Liu
keywords: Kanamycin ototoxicity; MiR204; sensorineural hearing loss; spiral ganglion neurons TMPRSS3