title: Hsa-miR-1246 and hsa-miR-1290 are associated with stemness and invasiveness of non-small cell lung cancer.
abstract: OBJECTIVE
  Cancer stem cells (CSCs) are considered to play a pivotal role in the process of invasion, metastasis and chemotherapy resistance. Diverse aberrantly expressed microRNAs (miRNAs) have been reported in lung cancer cells. However, there have been few reports about miRNAs that were associated with stemness and invasion of lung cancer. We investigated the role of miRNAs associated with characteristics of CSCs in non-small cell lung cancer (NSCLC).
  
  
  METHODS
  We cultured A549 cells (lung adenocarcinoma) and HCC1588 cells (lung squamous cell carcinoma) in serum free media condition. We isolated sphere-forming NSCLC cells and examined the microRNA expression by microarray and qRT-PCT. By inhibition of CSC-associated microRNAs, we identified the changes of stemness and invasiveness in NSCLC.
  
  
  CONCLUSIONS
  We discovered 44 over-expressed, 42 down-regulated miRNAs in the sphere-forming cells compared with the parent cells of NSCLC. By in-silico database search, we selected miR-1246 and miR-1290 that were suspected to be associated with CSCs among aberrantly expressed miRNAs. Inhibition of miR-1246 and miR-1290 showed decreased stemness markers and epithelial-mesenchymal transition (EMT) markers in NSCLC. Anti-miR-1246 and anti-miR-1290 suppressed proliferation, sphere-formation, colony formation and invasion of NSCLC. CSCs-associated miR-1246, or miR-1290 may be important in the invasion or metastasis of NSCLC.
authors.affiliation: Department of Pathology, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea.
authors.forename: Gwangil
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Pathology, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: An
authors.affiliation: Institute for Clinical Research, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea.
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Institute for Clinical Research, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea.
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Song
authors.affiliation: Institute for Clinical Research, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea.
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, CHA Bundang Medical Center, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, CHA Bundang Medical Center, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea. Electronic address: jhcmd@hanmail.net.
authors.forename: Hye-Cheol
authors.initials: HC
authors.lastname: Jeong
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D016895:Culture Media, Serum-Free; C577700:MIRN1246 microRNA, human; C574604:MIRN1290 microRNA, human; D035683:MicroRNAs
keywords: Cancer stem cell; MicroRNA; Non-small cell lung cancer