title: A simple novel approach for real-time monitoring of sodium gluconate production by on-line physiological parameters in batch fermentation by Aspergillus niger.
abstract: In this paper, approach for real-time monitoring of sodium gluconate (SG) fermentation was established for the first time by the equations which can calculate real-time key-parameters by on-line physiological data. Based on this approach, limiting factors were found out in initial fermentation F1 and then step-wise agitation increase and improved medium recipe were proposed in fermentation F2 and F3, respectively. The highest average SG production rate (16.58±0.91 g L(-1) h(-1)) was achieved in fermentation F3, which was 104.2% and 48.0% higher than those in fermentation F1 and F2, respectively. Meanwhile, due to shorter fermentation period (decreased from 34 h to 18.7 h), lower biomass (about 1.5 g L(-1)) and less by-product accumulation, the overall yield of 0.943±0.012 (mol mol(-1)) in fermentation F3 increased more than 16.0% compared to fermentation F1. This approach had been successfully applied to industrial fermentation and greatly improved SG production.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. Box 329, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. Box 329, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Zejian
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shan Dong Fuyang Biological Technology Co., Ltd, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. Box 329, 130 Meilong Road, Shanghai 200237, China. Electronic address: juchu@ecust.edu.cn.
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. Box 329, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
mesh_terms: D001234:Aspergillus niger / Q000502:physiology*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D003199:Computer Systems; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism*; D009862:Online Systems; D012212:Rheology; D013997:Time Factors; D014783:Viscosity
chemical_list: D005942:Gluconates; C030691:gluconic acid
keywords: Aspergillus niger; On-line parameters; Oxygen limited; Real-time monitoring; Sodium gluconate