title: Co-Carriage of blaKPC-2 and blaNDM-1 in Clinical Isolates of Pseudomonas aeruginosa Associated with Hospital Infections from India.
abstract: Global spread of KPC poses to be a serious threat complicating treatment options in hospital settings. The present study investigates the genetic environment of blaKPC-2 among clinical isolates of Pseudomonas aeruginosa from a tertiary referral hospital of India. The study isolates were collected from different wards and clinics of Silchar Medical College and Hospital, India, from 2012-2013. The presence of blaKPC was confirmed by genotypic characterization followed by sequencing. Cloning of the blaKPC-2 gene was performed and the genetic environment of this gene was characterized as well. Transferability of the resistance gene was determined by transformation assay and Southern hybridization. Additionally, restriction mapping was also carried out. Two isolates of P. aeruginosa were found to harbor blaKPC-2, were resistant towards aminoglycosides, quinolone and β-lactam-β-lactamase inhibitor combination. In both the isolates, the resistance determinant was associated with class 1 integron and horizontally transferable. Both the isolates were co-harboring blaNDM-1. The first detection of this integron mediated blaKPC-2 coexisting with blaNDM-1 in P. aeruginosa from India is worrisome, and further investigation is required to track the gene cassette mediated blaKPC-2 in terms of infection control and to prevent the spread of this gene in hospitals as well as in the community.
authors.affiliation: Department of Microbiology, Assam University, Silchar, India.
authors.forename: Deepjyoti
authors.initials: D
authors.lastname: Paul
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.
authors.forename: Debadatta
authors.initials: D
authors.lastname: Dhar Chanda
authors.affiliation: Department of Microbiology, Assam University, Silchar, India.
authors.forename: Anand Prakash
authors.initials: AP
authors.lastname: Maurya
authors.affiliation: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Chakravarty
authors.affiliation: Department of Life science and Bioinformatics, Assam University, Silchar, India.
authors.forename: Gauri Dutt
authors.initials: GD
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, Assam University, Silchar, India.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Bhattacharjee
mesh_terms: D000293:Adolescent; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003428:Cross Infection / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D007194:India; D008297:Male; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D062606:Tertiary Care Centers; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase