title: Improved Xylitol Production from D-Arabitol by Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter oxydans.
abstract: Gluconobacter oxydans is used to produce xylitol from D-arabitol. This study aims to improve xylitol production by increasing the coenzyme regeneration efficiency of the pentose phosphate pathway in G. oxydans. Glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) were overexpressed in G. oxydans. Real-time PCR and enzyme activity assays revealed that G6PDH/6PGDH activity and coenzyme regeneration efficiency increased in the recombinant G. oxydans strains. Approximately 29.3 g/L xylitol was obtained, with a yield of 73.2%, from 40 g/L d-arabitol in the batch biotransformation with the G. oxydans PZ strain. Moreover, the xylitol productivity (0.62 g/L/h) was 3.26-fold of the wild type strain (0.19 g/L/h). In repetitive batch biotransformation, the G. oxydans PZ cells were used for five cycles without incurring a significant loss in productivity. These results indicate that the recombinant G. oxydans PZ strain is economically feasible for xylitol production in industrial bioconversion.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology , Nanjing 210009, People's Republic of China.|College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology , Nanjing 210009, People's Republic of China.|College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology , Nanjing 210009, People's Republic of China.|College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Feng
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D050542:D-Xylulose Reductase; D020600:Gluconobacter oxydans / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D013402:Sugar Alcohols / Q000378:metabolism*; D014993:Xylitol / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D013402:Sugar Alcohols; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D050542:D-Xylulose Reductase; D014993:Xylitol; C014999:arabitol
keywords: Gluconobacter oxydans; coenzyme regeneration; pentose phosphate pathway; xylitol