title: Inhibition of Hedgehog-Signaling Driven Genes in Prostate Cancer Cells by Sutherlandia frutescens Extract.
abstract: Sutherlandia frutescens (L) R. Br. (Sutherlandia) is a South African botanical that is traditionally used to treat a variety of health conditions, infections and diseases, including cancer. We hypothesized Sutherlandia might act through Gli/ Hedgehog (Hh)-signaling in prostate cancer cells and used RNA-Seq transcription profiling to profile gene expression in TRAMPC2 murine prostate cancer cells with or without Sutherlandia extracts. We found 50% of Hh-responsive genes can be repressed by Sutherlandia ethanol extract, including the canonical Hh-responsive genes Gli1 and Ptch1 as well as newly distinguished Hh-responsive genes Hsd11b1 and Penk.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America.|MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.|Xiphophorus Genetic Stock Center, Texas State University, San Marcos, TX 78666, United States of America.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America.|MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Starkey
authors.affiliation: MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.|Division of Animal Science, University of Missouri, Columbia, MO 65211, United States of America.
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.affiliation: MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.|Computer Science Department, University of Missouri, Columbia, MO 65211, United States of America.|Informatics Institute, University of Missouri, Columbia, MO 65211, United States of America.
authors.forename: Jilong
authors.initials: J
authors.lastname: Li
authors.affiliation: MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.|Computer Science Department, University of Missouri, Columbia, MO 65211, United States of America.|Informatics Institute, University of Missouri, Columbia, MO 65211, United States of America.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America.|MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.
authors.forename: William R
authors.initials: WR
authors.lastname: Folk
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America.|MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America.
authors.forename: Dennis B
authors.initials: DB
authors.lastname: Lubahn
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D007887:Fabaceae / Q000737:chemistry*; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D015398:Signal Transduction / Q000187:drug effects*; D059467:Transcriptome / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D053823:Hedgehog Proteins; D010936:Plant Extracts