title: Growth, photosynthesis, and defense mechanism of antimony (Sb)-contaminated Boehmeria nivea L.
abstract: Ramie (Boehmeria nivea L.) is the oldest cash fiber crop in China and is widely grown in antimony (Sb) mining areas. To evaluate the extent of Sb resistance and tolerance, the growth, tolerance index (TI), Sb content in plant parts and in Hoagland solution, bioaccumulation factor (BF), photosynthesis, and physiological changes in Sb-contaminated B. nivea (20, 40, 80, and 200 mg L(-1) Sb) grown hydroponically were investigated. The Sb tolerance and resistance of ramie were clearly revealed by growth inhibition, a TI between 13 and 99 %, non-significant changes in the maximum quantum efficiency of photosystem (F v /F m ), energy-harvesting efficiency (photosystem II (PSII)) and single-photon avalanche diode (SPAD) value, a significant increase in Sb in plant parts, BF >1, and an increase in catalase (CAT) and malondialdehyde (MDA) at 200 mg L(-1) Sb. Under increasing Sb stress, nearly the same non-significant decline in the maximum quantum efficiency of photosystem (F v /F m ), energy-harvesting efficiency (PSII), relative quantum yield of photosystem II (φPSII), and photochemical quenching (qP), except for F v /F m at 20 mg L(-1) Sb, were recorded. SPAD values for chlorophyll under Sb stress showed an increasing trend, except for a slight decrease, i.e., <2 %, than the control SPAD value at 200 mg L(-1) Sb. With a continuous increase in MDA, superoxide dismutase (SOD), peroxidase (POD), and CAT activities were suppressed under Sb addition up to 40 mg L(-1) Sb and the addition of Sb enhanced enzyme production at 80 and 200 mg L(-1) Sb. A continuous decrease in SOD, POD, and CAT up to 40 mg L(-1) Sb and enhancements at ≥80 mg L(-1), along with the continuous enhancement of MDA activity and inhibited biomass production, clearly reveal the roles of these enzymes in detoxifying Sb stress and the defense mechanism of ramie at 80 mg L(-1) Sb. Thus, B. nivea constitutes a promising candidate for Sb phytoremediation at mining sites.
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, Hunan, 410083, China.|National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China.
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Chai
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, Hunan, 410083, China.|National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China.
authors.forename: Hussani
authors.initials: H
authors.lastname: Mubarak
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, Hunan, 410083, China.|National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Yang
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, Hunan, 410083, China.|National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yong
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, Hunan, 410083, China.|National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China.
authors.forename: Chong-Jian
authors.initials: CJ
authors.lastname: Tang
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, Hunan, 410083, China. nosheen_mirza@ciit.net.pk.|National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China. nosheen_mirza@ciit.net.pk.|Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan. nosheen_mirza@ciit.net.pk.
authors.forename: Nosheen
authors.identifier: http://orcid.org/0000-0003-0899-7370
authors.initials: N
authors.lastname: Mirza
mesh_terms: D000965:Antimony / Q000633:toxicity*; D001673:Biodegradation, Environmental; D032402:Boehmeria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002681:China; D002734:Chlorophyll / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008906:Mining; D009195:Peroxidase / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D012989:Soil Pollutants; D002734:Chlorophyll; D008315:Malondialdehyde; D000965:Antimony; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: Antimony (Sb); Antioxidant enzymes; Chlorophyll fluorescence; Phytoremediation; Ramie