title: Comprehensive systems biology analysis of a 7-month cigarette smoke inhalation study in C57BL/6 mice.
abstract: Smoking of combustible cigarettes has a major impact on human health. Using a systems toxicology approach in a model of chronic obstructive pulmonary disease (C57BL/6 mice), we assessed the health consequences in mice of an aerosol derived from a prototype modified risk tobacco product (pMRTP) as compared to conventional cigarettes. We investigated physiological and histological endpoints in parallel with transcriptomics, lipidomics, and proteomics profiles in mice exposed to a reference cigarette (3R4F) smoke or a pMRTP aerosol for up to 7 months. We also included a cessation group and a switching-to-pMRTP group (after 2 months of 3R4F exposure) in addition to the control (fresh air-exposed) group, to understand the potential risk reduction of switching to pMRTP compared with continuous 3R4F exposure and cessation. The present manuscript describes the study design, setup, and implementation, as well as the generation, processing, and quality control analysis of the toxicology and 'omics' datasets that are accessible in public repositories for further analyses.
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Sam
authors.initials: S
authors.lastname: Ansari
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Karine
authors.initials: K
authors.lastname: Baumer
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boué
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Sophie
authors.initials: S
authors.lastname: Dijon
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Remi
authors.initials: R
authors.lastname: Dulize
authors.affiliation: Zora Biosciences Oy, Biologinkuja 1, Espoo 02150, Finland.
authors.forename: Kim
authors.initials: K
authors.lastname: Ekroos
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Elamin
authors.affiliation: Philip Morris International Research Laboratories, 50 Science Park Road, Science Park II, Singapore, Singapore 117406.
authors.forename: Clement
authors.initials: C
authors.lastname: Foong
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guedj
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Julia
authors.initials: J
authors.lastname: Hoeng
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ivanov
authors.affiliation: Philip Morris International Research Laboratories, 50 Science Park Road, Science Park II, Singapore, Singapore 117406.
authors.forename: Subash
authors.initials: S
authors.lastname: Krishnan
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Patrice
authors.initials: P
authors.lastname: Leroy
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Martin
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Celine
authors.initials: C
authors.lastname: Merg
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Peck
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Manuel C
authors.initials: MC
authors.lastname: Peitsch
authors.affiliation: Philip Morris International Research Laboratories, 50 Science Park Road, Science Park II, Singapore, Singapore 117406.
authors.forename: Blaine
authors.initials: B
authors.lastname: Phillips
authors.affiliation: Biology consultant, Max-Baermann-Str. 21, 51429 Bergisch Gladbach, Germany.
authors.forename: Walter K
authors.initials: WK
authors.lastname: Schlage
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schneider
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Marja
authors.initials: M
authors.lastname: Talikka
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Bjoern
authors.initials: B
authors.lastname: Titz
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Vanscheeuwijck
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Emilija
authors.initials: E
authors.lastname: Veljkovic
authors.affiliation: Zora Biosciences Oy, Biologinkuja 1, Espoo 02150, Finland.
authors.forename: Terhi
authors.initials: T
authors.lastname: Vihervaara
authors.affiliation: Philip Morris International R&D, Philip Morris Products S. A., Quai Jeanrenaud 5, Neuchâtel 2000, Switzerland.
authors.forename: Gregory
authors.initials: G
authors.lastname: Vuillaume
authors.affiliation: Philip Morris International Research Laboratories, 50 Science Park Road, Science Park II, Singapore, Singapore 117406.
authors.forename: Ching Qing
authors.initials: CQ
authors.lastname: Woon
mesh_terms: D000818:Animals; D001835:Body Weight; D005260:Female; D050356:Lipid Metabolism; D008168:Lung / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D040081:Protein Array Analysis; D040901:Proteomics; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D015208:Smoke Inhalation Injury / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D012907:Smoking / Q000009:adverse effects*