title: A Wash-Free Homogeneous Colorimetric Immunoassay Method.
abstract: Rapid and convenient biosensing platforms could be beneficial to timely diagnosis and treatment of diseases in virtually any care settings. Sandwich immunoassays, the most commonly used methods for protein detection, often rely on expensive tags such as enzyme and tedious wash and incubation procedures operated by skilled labor. In this report, we revolutionized traditional sandwich immunoassays by providing a wash-free homogeneous colorimetric immunoassay method without requirement of any separation steps. The proposed strategy was realized by controlling the growth of gold nanoparticles (AuNPs) to mediate the interparticle spacing in the protein-AuNP oligomers. We have demonstrated the successful in vitro detection of cancer biomarker in serum samples from patients with high clinical sensitivity and specificity.
authors.affiliation: 1. College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Huiqiao
authors.initials: H
authors.lastname: Liu
authors.affiliation: 2. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Rong
authors.affiliation: 3. Key Laboratory of Optical Information Science and Technology, Ministry of Education, Institute of Modern Optics, Nankai University, Tianjin 300071, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Jia
authors.affiliation: 1. College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: 1. College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.affiliation: 2. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Dong
authors.affiliation: 2. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Cejun
authors.initials: C
authors.lastname: Yang
authors.affiliation: 2. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Pengzhi
authors.initials: P
authors.lastname: Hu
authors.affiliation: 2. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: 3. Key Laboratory of Optical Information Science and Technology, Ministry of Education, Institute of Modern Optics, Nankai University, Tianjin 300071, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Liu
authors.affiliation: 1. College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Dingbin
authors.initials: D
authors.lastname: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053758:Nanoparticles / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D006046:Gold
keywords: clinical diagnostics; colorimetric immunoassay; gold nanoparticles