title: miR-26b-3p Regulates Human Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation by Targeting Estrogen Receptor.
abstract: Human umbilical cord-derived mesenchymal stem cells (hUC-MSC) have been considered as promising candidates for cell-based regeneration medicine. However, the application was limited to its poor in vitro proliferation ability against the huge demand of cells. MicroRNA plays important roles in the regulation of cell proliferation, apoptosis, and differentiation. The objective of this study is to explore the roles of miRNAs in regulating the in vitro proliferation of hUC-MSC and unveil their possible mechanism. In this study, we found that miR-26b-3p was significantly upregulated during serial in vitro passage of hUC-MSC and was correlated with cellular senescence and cell cycle genes. The overexpression of miR-26b-3p greatly inhibited the proliferation of hUC-MSC in vitro, which is indicated by 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay, cell cycle, and cell growth curve analyses. miR-26b-3p suppression partly rescued this phenotype by maintaining its proliferation ability in vitro. For mechanism studies, we predicted and validated that miR-26b-3p suppresses estrogen receptor 1 (ESR1) expression by directly binding to the coding sequence (CDS) region of its message RNA (mRNA), thus subsequently changing the expression of its downstream effector Cyclin D1. In conclusion, we found that miR-26b-3p played an important role in the regulation of hUC-MSC proliferation in vitro by targeting the ESR-CCND1 pathway.
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .|3 Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Xu
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Junfeng
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Binghao
authors.initials: B
authors.lastname: Yan
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Daolan
authors.initials: D
authors.lastname: Gu
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Minjuan
authors.initials: M
authors.lastname: Wu
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China .|2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Houqi
authors.initials: H
authors.lastname: Liu
mesh_terms: D050156:Adipogenesis; D001483:Base Sequence; D002454:Cell Differentiation; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000235:genetics; D019938:Cyclin D1 / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D019938:Cyclin D1; D035683:MicroRNAs; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C530104:CCND1 protein, human; C528163:MIRN26 microRNA, human