title: Expression of microRNAs in Horse Plasma and Their Characteristic Nucleotide Composition.
abstract: MicroRNAs (miRNAs) in blood plasma are stable under high levels of ribonuclease activity and could function in tissue-to-tissue communication, suggesting that they may have distinctive structural characteristics compared with non-circulating miRNAs. In this study, the expression of miRNAs in horse plasma and their characteristic nucleotide composition were examined and compared with non-plasma miRNAs. Highly expressed plasma miRNA species were not part of the abundant group of miRNAs in non-plasma tissues, except for the eca-let-7 family. eca-miR-486-5p, -92a, and -21 were among the most abundant plasma miRNAs, and their human orthologs also belong to the most abundant group of miRNAs in human plasma. Uracil and guanine were the most common nucleotides of both plasma and non-plasma miRNAs. Cytosine was the least common in plasma and non-plasma miRNAs, although levels were higher in plasma miRNAs. Plasma miRNAs also showed higher expression levels of miRNAs containing adenine and cytosine repeats, compared with non-plasma miRNAs. These observations indicate that miRNAs in the plasma have a unique nucleotide composition.
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Seungwoo
authors.initials: S
authors.lastname: Lee
authors.affiliation: Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, South Korea.
authors.forename: Seungwoo
authors.initials: S
authors.lastname: Hwang
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Yu
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Dayoung
authors.initials: D
authors.lastname: Oh
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Yu Jung
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Myung-Chul
authors.initials: MC
authors.lastname: Kim
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Yongbaek
authors.initials: Y
authors.lastname: Kim
authors.affiliation: BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
authors.forename: Doug-Young
authors.initials: DY
authors.lastname: Ryu
mesh_terms: D000818:Animals; D006736:Horses; D035683:MicroRNAs / Q000097:blood / Q000378:metabolism*; D009711:Nucleotides / Q000097:blood / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D009711:Nucleotides