title: A Novel Marker for Purkinje Cells, Ribosomal Protein MPS1/S27: Expression of MPS1 in Human Cerebellum.
abstract: BACKGROUND
  The ribosomal protein metallopanstimulin-1 (MPS1/S27) serves critical survival purposes in cell division, in normal and cancerous cells; for this reason, selective pressures of evolution have conserved the DNA sequences encoding MPS1/S27 in Archaea and Eukariotic cells. The expression of MPS1/S27 protein in human adult cerebellum has not been established.
  
  
  METHODS
  The presence of MPS1/S27, was screened in paraffin-embedded human adult brain specimens processed for tissue inmunohistochemistry. Affinity-purified specific antibodies were directed against the N-terminus of MPS1.
  
  
  RESULTS
  The antibodies to MPS1 detected Purkinje cells (PC) and their dendrites. In PC, MPS1 antigen-positive staining was found in: the nucleolus, which was strongly stained; ribosomes attached to the external nuclear membrane; cytoplasm of PC, with strong staining in a punctuate fashion; the soma-attached large dendrite trunks of PC, which were MPS1 antigen-positive; and the granular cell layer, where cellular staining in a few cells that appeared to resemble smaller PC was observed.
  
  
  CONCLUSIONS
  Since MPS1 is involved in cell division, DNA repair, and ribosomal biogenesis, it may be a useful antigen for studying processes such as protein synthesis, oncogenesis, regeneration, aging, and perhaps diseases of the human cerebellum.
authors.affiliation: Antagoras Agrobusiness, LLC, Chesterfield, MO, U.S.A. fernandezpol@att.net.
authors.forename: J Alberto
authors.initials: JA
authors.lastname: Fernandez-Pol
mesh_terms: D000368:Aged; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008667:Metalloproteins / Q000378:metabolism*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D008667:Metalloproteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D012269:Ribosomal Proteins
keywords: DNA repair protein; Metallopanstimulin-1; Purkinje cells; RP; cerebellum; immunohistochemistry marker; p53; ribosomal protein; ribosomal protein MPS1/S27; zinc-finger proteins