title: Phenotype-specific apoptosis induced by three new triterpenoid saponins from Albizia glaberrima (Schumach. & Thonn.) Benth.
abstract: As part of our search of new bioactive saponins from Cameroonian medicinal plants, phytochemical investigation of the roots of Albizia glaberrima led to the isolation of three new oleanane-type saponins, named glaberrimosides A-C (1-3). Their structures were established by direct interpretation of their spectral data, mainly HRESIMS, 1D NMR (1H, 13C NMR, and DEPT) and 2D NMR (COSY, ROESY, HSQC and HMBC) as 3-O-[α-L-arabinopyranosyl-(1 → 6)-[β-D-glucopyranosyl-(1 → 2)]-β-D-glucopyranosyl]-28-O-[β-D-glucopyranosyl-(1 → 6)-[β-d-glucopyranosyl-(1 → 2)]-β-D-glucopyranosyl]-oleanolic acid (1), 3-O-[α-L-arabinopyranosyl-(1 → 6)-[β-D-glucopyranosyl-(1 → 2)]-β-D-glucopyranosyl]-28-O-[β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl]-oleanolic acid (2), and 3-O-[β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl]-28-O-[β-D-glucopyranosyl-(1 → 6)-[β-D-fucopyranosyl-(1 → 2)]-β-D-glucopyranosyl]-oleanolic acid (3). The pro-apoptotic effect of the three saponins was evaluated on three human cell lines (pancreatic carcinoma AsPC-1, hematopoietic monocytic THP-1, and human fibroblast cell line BJ). Saponins 1-3 specifically induced apoptosis of pancreatic carcinoma cell (AsPC-1) in a dose-dependent manner. More interestingly, there were inactive on monocytic (THP-1) and normal human fibroblast (BJ) cell lines.
authors.affiliation: Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé, BP 812 Yaoundé, Cameroun. Electronic address: oliviernote1@yahoo.fr.
authors.forename: Olivier Placide
authors.initials: OP
authors.lastname: Noté
authors.affiliation: Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors.forename: Sarah Ali
authors.initials: SA
authors.lastname: Azouaou
authors.affiliation: Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé, BP 812 Yaoundé, Cameroun.
authors.forename: Line
authors.initials: L
authors.lastname: Simo
authors.affiliation: Service Commun d'Analyse, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Antheaume
authors.affiliation: Laboratoire de Chimie Thérapeutique, UMR7312, 51 rue Cognacq-Jay, 51100 Reims, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Guillaume
authors.affiliation: Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé, BP 812 Yaoundé, Cameroun.
authors.forename: Dieudonné Emmanuel
authors.initials: DE
authors.lastname: Pegnyemb
authors.affiliation: Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors.forename: Christian Dominique
authors.initials: CD
authors.lastname: Muller
authors.affiliation: Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors.forename: Annelise
authors.initials: A
authors.lastname: Lobstein
mesh_terms: D031289:Albizzia / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D012503:Saponins; D014315:Triterpenes; C000608979:glaberrimoside A; C000608980:glaberrimoside B; C000608981:glaberrimoside C
keywords: Albizia glaberrima; Mimosaceae; Pancreatic carcinoma AsPC-1; Pro-apoptotic activity; Triterpenoid saponins