title: Insights into the plant polysaccharide degradation potential of the xylanolytic yeast Pseudozyma brasiliensis.
abstract: In second-generation (2G) bioethanol production, plant cell-wall polysaccharides are broken down to release fermentable sugars. The enzymes of this process are classified as carbohydrate-active enzymes (CAZymes) and contribute substantially to the cost of biofuel production. A novel basidiomycete yeast species, Pseudozyma brasiliensis, was recently discovered. It produces an endo-β-1,4-xylanase with a higher specific activity than other xylanases. This enzyme is essential for the hydrolysis of biomass-derived xylan and has an important role in 2G bioethanol production. In spite of the P. brasiliensis biotechnological potential, there is no information about how it breaks down polysaccharides. For the first time, we characterized the secretome of P. brasiliensis grown on different carbon sources (xylose, xylan, cellobiose and glucose) and also under starvation conditions. The growth and consumption of each carbohydrate and the activity of the CAZymes of culture supernatants were analyzed. The CAZymes found in its secretomes, validated by enzymatic assays, have the potential to hydrolyze xylan, mannan, cellobiose and other polysaccharides. The data show that this yeast is a potential source of hydrolases, which can be used for biomass saccharification.
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Av Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brasil.
authors.forename: Antonio Adalberto
authors.initials: AA
authors.lastname: Kaupert Neto
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Av Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brasil.
authors.forename: Gustavo Pagotto
authors.initials: GP
authors.lastname: Borin
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Av Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brasil Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brasil.
authors.forename: Gustavo Henrique
authors.initials: GH
authors.lastname: Goldman
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Av Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brasil Departamento de Bioquímica e Biologia Tecidual, Instituto de Biologia, Universidade de Campinas, Rua Monteiro Lobato, 255, Caixa Postal 6109, CEP 13083-970, São Paulo, Brasil.
authors.forename: André Ricardo de Lima
authors.initials: AR
authors.lastname: Damásio
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Av Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brasil juliana.velasco@bioetanol.org.br.
authors.forename: Juliana Velasco de Castro
authors.initials: JV
authors.lastname: Oliveira
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000378:metabolism*; D014587:Ustilaginales / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D000431:Ethanol; D006026:Glycoside Hydrolases
keywords: Basidiomycete; CAZyme; Pseudozyma brasiliensis; second-generation bioethanol; secretome; xylanolytic yeast