title: Spectrophotometric and chemometric methods for determination of imipenem, ciprofloxacin hydrochloride, dexamethasone sodium phosphate, paracetamol and cilastatin sodium in human urine.
abstract: New accurate, sensitive and selective spectrophotometric and chemometric methods were developed and subsequently validated for determination of Imipenem (IMP), ciprofloxacin hydrochloride (CIPRO), dexamethasone sodium phosphate (DEX), paracetamol (PAR) and cilastatin sodium (CIL) in human urine. These methods include a new derivative ratio method, namely extended derivative ratio (EDR), principal component regression (PCR) and partial least-squares (PLS) methods. A novel EDR method was developed for the determination of these drugs, where each component in the mixture was determined by using a mixture of the other four components as divisor. Peak amplitudes were recorded at 293.0 nm, 284.0 nm, 276.0 nm, 257.0 nm and 221.0 nm within linear concentration ranges 3.00-45.00, 1.00-15.00, 4.00-40.00, 1.50-25.00 and 4.00-50.00 μg mL(-1) for IMP, CIPRO, DEX, PAR and CIL, respectively. PCR and PLS-2 models were established for simultaneous determination of the studied drugs in the range of 3.00-15.00, 1.00-13.00, 4.00-12.00, 1.50-9.50, and 4.00-12.00 μg mL(-1) for IMP, CIPRO, DEX, PAR and CIL, respectively, by using eighteen mixtures as calibration set and seven mixtures as validation set. The suggested methods were validated according to the International Conference of Harmonization (ICH) guidelines and the results revealed that they were accurate, precise and reproducible. The obtained results were statistically compared with those of the published methods and there was no significant difference.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: El-Kosasy
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
authors.forename: Omar
authors.initials: O
authors.lastname: Abdel-Aziz
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
authors.forename: N
authors.initials: N
authors.lastname: Magdy
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt. Electronic address: noha_morsy_elzahar@hotmail.com.
authors.forename: N M
authors.initials: NM
authors.lastname: El Zahar
mesh_terms: D000082:Acetaminophen / Q000652:urine*; D018712:Analgesics, Non-Narcotic / Q000652:urine*; D000900:Anti-Bacterial Agents / Q000652:urine*; D000893:Anti-Inflammatory Agents / Q000652:urine*; D002939:Ciprofloxacin / Q000652:urine*; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D015378:Imipenem / Q000652:urine*; D016018:Least-Squares Analysis; D057230:Limit of Detection; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D018712:Analgesics, Non-Narcotic; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; C004180:dexamethasone 21-phosphate; D000082:Acetaminophen; D002939:Ciprofloxacin; D015378:Imipenem; D003907:Dexamethasone
keywords: Extended derivative ratio; Human urine; Multivariate calibration; PCR; PLS-2