title: The complete mitochondrial genome sequence of the Dark-spotted frog Pelophylax nigromaculatus (Amphibia, Anura, Ranidae).
abstract: The dark-spotted frog (Pelophylax nigromaculatus) belongs to Ranidae. This species is known from the Russian Far East, central, northern and north-eastern China, the Democratic People's Republic of Korea, the Republic of Korea, and Japan. In this study, the complete mitochondrial genome of P. nigromaculatus was sequenced. The mitogenome was 17 567 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a non-coding control region. As in other vertebrates, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall base composition of the P. nigromaculatus is 29.2% A, 27.4% T, 28.4% C, and 15.0% G. Phylogenetic analysis showed P. nigromaculatus was closely related to P. plancyi and P. chosenicus. The complete mitogenome of P. nigromaculatus can provide important data for the studies on phylogenetic relationship and population genetics to further explore the taxonomic status of this species.
authors.affiliation: a Key Laboratory for Molecular Biology and Biopharmaceutics, School of Life Science and Technology , Mianyang Normal University , Mianyang , Sichuan , PR China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Jiang
authors.affiliation: a Key Laboratory for Molecular Biology and Biopharmaceutics, School of Life Science and Technology , Mianyang Normal University , Mianyang , Sichuan , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: b Department of Life Science and Engineering , Jining University of Shandong , Qufu , Shandong , PR China.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Key Laboratory for Molecular Biology and Biopharmaceutics, School of Life Science and Technology , Mianyang Normal University , Mianyang , Sichuan , PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Leng
authors.affiliation: a Key Laboratory for Molecular Biology and Biopharmaceutics, School of Life Science and Technology , Mianyang Normal University , Mianyang , Sichuan , PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: a Key Laboratory for Molecular Biology and Biopharmaceutics, School of Life Science and Technology , Mianyang Normal University , Mianyang , Sichuan , PR China.
authors.forename: Qiping
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D029845:Amphibian Proteins / Q000235:genetics; D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics; D005202:Asia, Eastern; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D008928:Mitochondria / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D010802:Phylogeny; D058974:Phylogeography; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D011898:Ranidae / Q000235:genetics*; D012426:Russia
chemical_list: D029845:Amphibian Proteins; D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Complete mitochondrial genome; Pelophylax nigromaculatus; Ranidae; protein-coding genes