title: Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation.
abstract: Sulfate-modifying factor 2 (SUMF2), a member of the formylglycine-generating enzyme family, was earlier found to play a role in the regulation of interleukin (IL)-13 expression and secretion in airway smooth muscle cells. IL-13 is a T helper 2 cytokine that plays important roles in the pathogenesis of asthma. However, there is little evidence of the potential role of SUMF2 in the cellular inflammatory responses in asthma. Here, using an ovalbumin-induced asthma rat model, we show that SUMF2 gene expression is significantly decreased in allergic asthma rats. Moreover, several pathological changes were observed in the lung tissue and IL-13 was found to be overexpressed in the ovalbumin-induced asthma model. Additional studies on the lung bronchial epithelial tissues, peripheral blood lymphocytes and bronchoalveolar lavage fluid of the OVA-induced asthma rats showed that SUMF2 mRNA and protein expression were attenuated. However, there was only a little significant correlation was found between SUMF2 and IL-13 expression. These results indicate that SUMF2 may mediate airway inflammation in allergic asthma by modulating the expression of IL-13. More data from in vivo experiments are needed to clearly understand the role of SUMF2 in asthma.
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Fang
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Guangqiang
authors.initials: G
authors.lastname: Gao
authors.affiliation: Department of Clinical Diagnosis, The Fourth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000485:Allergens / Q000633:toxicity; D000818:Animals; D001249:Asthma / Q000276:immunology*; D015153:Blotting, Western; D004195:Disease Models, Animal; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006967:Hypersensitivity / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D018793:Interleukin-13 / Q000096:biosynthesis*; D010047:Ovalbumin / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013429:Sulfatases / Q000096:biosynthesis* / Q000235:genetics; D059467:Transcriptome
chemical_list: D000485:Allergens; D018793:Interleukin-13; D010047:Ovalbumin; C000611408:SUMF2 protein, rat; D013429:Sulfatases
keywords: Allergic asthma; OVA; SUMF2 gene; airway smooth muscle cells; airways inflammation; interleukin-13