title: [Proteomic analysis of exhaled breath condensate for diagnosis of pathologies of the respiratory system].
abstract: Study of the proteomic composition of exhaled breath condensate (EBC), is a promising non-invasive method for the diagnosis of the respiratory tract diseases in patients. In this study the EBC proteomic composition of the 79 donors, including patients with different pathologies of the respiratory system has been investigated. Cytoskeletal keratins type II (1, 2, 3, 4, 5, 6) and cytoskeletal keratins the type I (9, 10, 14, 15, 16) were invariant for all samples. Analyzing the frequency of occurrence of proteins in different groups of examined patients, several categories of protein have been recognized: found in all pathologies (Dermcidin, Alpha-1-microglobulin, SHROOM3), found in several pathologies (CSTA, LCN1, JUP, PIP, TXN), and specific for a single pathology (PRDX1, Annexin A1/A2). The EBC analysis by HPLC-MS/MS can be used to identify potential protein markers characteristic for pathologies such as chronic obstructive pulmonary disease (PRDX1) and pneumonia (Annexin A1/A2).
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; Emanuel Institute of Biochemical Physics RAS, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Kononikhin
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Moscow, Russia; Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: K Yu
authors.initials: KY
authors.lastname: Fedorchenko
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Moscow, Russia; Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Ryabokon
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; Emanuel Institute of Biochemical Physics RAS, Moscow, Russia.
authors.forename: N L
authors.initials: NL
authors.lastname: Starodubtseva
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; Emanuel Institute of Biochemical Physics RAS, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Popov
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Zavialova
authors.affiliation: Research Institute of Pulmonology, Moscow, Russia.
authors.forename: E C
authors.initials: EC
authors.lastname: Anaev
authors.affiliation: Research Institute of Pulmonology, Moscow, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Chuchalin
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Moscow, Russia; Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: S D
authors.initials: SD
authors.lastname: Varfolomeev
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaev
mesh_terms: D000328:Adult; D000368:Aged; D001944:Breath Tests / Q000379:methods; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D012140:Respiratory Tract Diseases / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology
chemical_list: D003598:Cytoskeletal Proteins
keywords: chronic obstructive pulmonary disease (COPD); exhaled breath condensate; mass spectrometry; pneumonia; proteomics