title: TOMATOMA Update: Phenotypic and Metabolite Information in the Micro-Tom Mutant Resource.
abstract: TOMATOMA (http://tomatoma.nbrp.jp/) is a tomato mutant database providing visible phenotypic data of tomato mutant lines generated by ethylmethane sulfonate (EMS) treatment or γ-ray irradiation in the genetic background of Micro-Tom, a small and rapidly growing variety. To increase mutation efficiency further, mutagenized M3 seeds were subjected to a second round of EMS treatment; M3M1 populations were generated. These plants were self-pollinated, and 4,952 lines of M3M2 mutagenized seeds were generated. We checked for visible phenotypes in the M3M2 plants, and 618 mutant lines with 1,194 phenotypic categories were identified. In addition to the phenotypic information, we investigated Brix values and carotenoid contents in the fruits of individual mutants. Of 466 samples from 171 mutant lines, Brix values and carotenoid contents were between 3.2% and 11.6% and 6.9 and 37.3 µg g(-1) FW, respectively. This metabolite information concerning the mutant fruits would be useful in breeding programs as well as for the elucidation of metabolic regulation. Researchers are able to browse and search this phenotypic and metabolite information and order seeds of individual mutants via TOMATOMA. Our new Micro-Tom double-mutagenized populations and the metabolic information could provide a valuable genetic toolkit to accelerate tomato research and potential breeding programs.
authors.affiliation: Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan ezura@gene.tsukuba.ac.jp.
authors.forename: Masahito
authors.initials: M
authors.lastname: Shikata
authors.affiliation: Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Hoshikawa
authors.affiliation: Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Ariizumi
authors.affiliation: Agricultural and Forestry Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8577, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Fukuda
authors.affiliation: National Institute of Genetics, Mishima, 411-8540, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yamazaki
authors.affiliation: Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan ezura@gene.tsukuba.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
mesh_terms: D001947:Breeding; D030541:Databases, Genetic; D005020:Ethyl Methanesulfonate; D005638:Fruit / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D012639:Seeds / Q000235:genetics / Q000378:metabolism
chemical_list: D005020:Ethyl Methanesulfonate
keywords: Database; Fruit; Micro-Tom; Mutant; Resource; Tomato