title: Decreases in plasma MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios in uremic patients during hemodialysis.
abstract: BACKGROUND
  Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors (TIMPs) play important roles in the pathophysiology of renal diseases. Imbalanced MMPs/TIMPs are implicated in the vascular alterations of uremic patients on hemodialysis (HD). We have investigated the plasma levels of MMP-2, MMP-9, TIMP-1 and TIMP-2 in uremic patients and the effects of a course of HD on the changes in these factors.
  
  
  METHODS
  There were 382 uremic patients on regular HD treatment and 50 healthy controls enrolled in this study. The plasma MMP-2 and MMP-9 levels were detected by gelatin zymography, and TIMP-1 and TIMP-2 concentrations were determined by ELISA assay.
  
  
  RESULTS
  Significantly higher plasma MMP-2 and MMP-9 and decreased TIMP-1 in the uremic patients were detected compared with those in the controls. Therefore, there were markedly higher MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios in the uremic patients. In the course of a single HD session, the plasma MMP-2 level was significantly decreased from pre-HD to post-HD. TIMP-1 concentration was significantly increased from pre-HD to post-HD. Although the HD session did not have a significant effect on the levels of plasma MMP-9 and TIMP-2, both plasma MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios were significantly decreased from pre-HD to post-HD levels.
  
  
  CONCLUSIONS
  HD session could decrease MMP-2 and increase TIMP-1 level in the circulation of uremic patients. The physiological significance of reduced MMPs/TIMPs ratio due to a single HD session is required to further validate.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, No. 75 Bo-Ai Street, Hsinchu, Taiwan.|Division of Nephrology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
authors.forename: Li-Che
authors.initials: LC
authors.lastname: Lu
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, No. 75 Bo-Ai Street, Hsinchu, Taiwan.|Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital Hsinchu Branch, Hsinchu, Taiwan.
authors.forename: Chung-Wei
authors.initials: CW
authors.lastname: Yang
authors.affiliation: Division of Chest Medicine, Department of Internal Medicine, Mackay Memorial Hospital, Hsinchu, Taiwan.
authors.forename: Wen-Yeh
authors.initials: WY
authors.lastname: Hsieh
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, No. 75 Bo-Ai Street, Hsinchu, Taiwan.
authors.forename: Wan-Hsuan
authors.initials: WH
authors.lastname: Chuang
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, No. 75 Bo-Ai Street, Hsinchu, Taiwan.
authors.forename: Yi-Chang
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, No. 75 Bo-Ai Street, Hsinchu, Taiwan. lincs@mail.nctu.edu.tw.
authors.forename: Chih-Sheng
authors.initials: CS
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D006435:Renal Dialysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood*; D014511:Uremia / Q000097:blood* / Q000628:therapy
chemical_list: C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Hemodialysis; Matrix metalloproteinase; Tissue inhibitor of matrix metalloproteinase; Uremic patient