title: miR-31 Reduces Cell Growth of Papillary Thyroid Carcinoma by RNA-Binding Protein HuR.
abstract: BACKGROUND
  Increasing evidence suggests that microRNAs are widely involved in cancer progression and metastasis. However, the specific role of miR-31 in papillary thyroid carcinoma (PTC) is still largely unknown.
  
  
  METHODS
  The level of miR-31 and HuR was detected in 30 pairedcancerous and noncancerous tissue samples using real time PCR. The impact of miR-31 on PTC cell viability and apoptosis was explored using MTT assay and flow cytometry, respectively. To explore the effect of miR-31 on HuR expression, luciferase reporter assay was used.
  
  
  RESULTS
  In papillary thyroid carcinoma patients, miR-31 was significantly down regulated. Furthermore, down regulation of miR-31 increased the proliferation, migration, and invasion of ovarian carcinoma cells. Vice versa, over expression of miR-31 repressed cell invasion and viability. The luciferase reporter assay revealed that HuR was a target for miR-31. Further analysis defined that knockdown of HuR resulted in enhanced cell viability and decreased cell migration rate.
  
  
  CONCLUSIONS
  Down regulation of miR-31 contributed to the malignant progression of papillary thyroid carcinoma cells by targeting HuR.
authors.forename: Danping
authors.initials: D
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Shang
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Hongdan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D000067780:ELAV-Like Protein 1 / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; C534612:MIRN31 microRNA, human; D035683:MicroRNAs