title: Investigation of the composition and antibacterial activity of Ukrain™ drug using liquid chromatography techniques.
abstract: The greater celandine (Chelidonium majus L.) has been known for the centuries as a medicinal plant. One of the therapeutic agents based on C. majus is anticancer drug Ukrain™ known as a semi-synthetic C. majus alkaloid derivative. Although there are no doubts about antitumor properties of the drug, there is still controversy about its composition. In this study, Ukrain™ was subjected to TLC and LC-MS/MS analyses to compare it with C. majus alkaloid root extract and to determine its composition. Moreover, microbiological activity of both Ukrain™ and the alkaloid extract were tested against Bacillus subtilis strains using TLC-direct bioautography. Sanguinarine, chelidonine, α-homochelidonie and chelerythrine were found to have antibacterial properties. Besides chelidonine, sanguinarine, chelerythrine, protopine, allocryptopine, homochelidonie, berberine and coptisine reported earlier in literature, the presence of stylopine, norchelidonine, dihydrochelidonine and hydroberberine in Ukrain™ was detected, and here they have been reported for the first time.
authors.affiliation: Maria Curie-Skłodowska University, Department of Chromatographic Methods, Lublin, Poland.
authors.forename: Wioleta
authors.initials: W
authors.lastname: Jesionek
authors.affiliation: Laboratory of Separation and Spectroscopic Method Applications, Center for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Lublin, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Fornal
authors.affiliation: University of Life Sciences, Department of Veterinary Microbiology, Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Majer-Dziedzic
authors.affiliation: Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Ágnes M
authors.initials: ÁM
authors.lastname: Móricz
authors.affiliation: Nowicky Pharma, Vienna, Austria.
authors.forename: Wassil
authors.initials: W
authors.lastname: Nowicky
authors.affiliation: Maria Curie-Skłodowska University, Department of Chromatographic Methods, Lublin, Poland. Electronic address: irena.choma@umcs.lublin.pl.
authors.forename: Irena M
authors.initials: IM
authors.lastname: Choma
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects*; D001600:Berberine Alkaloids / Q000737:chemistry* / Q000494:pharmacology*; D002853:Chromatography, Liquid; D010617:Phenanthridines / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001600:Berberine Alkaloids; D010617:Phenanthridines; D010936:Plant Extracts; C060921:ukrain
keywords: Chelidonium majus L.; Isoquinoline alkaloids; LC–MS; TLC-DB; Ukrain™