title: A novel Mn(2+)-doped core/shell quantum dot-based intracellular probe for fluoride anions sensing in MC3T3-E1 osteoblastic cells.
abstract: In this paper, 3-aminobenzeneboronic acid functionalized Mn(2+)-doped ZnTe/ZnSe quantum dots (APBA-dQDs) were prepared. The APBA functional groups had strong binding ability with F(-), resulting in the quenchment of dQDs photoluminescence (PL). Under the optimal condition, the fluorescence intensity of APBA-dQDs was related linearly to the concentration of F(-) in the range of 0.25-1.5µmol/L with a detection limit of 0.1µmol/L. The selectivity of fluorescence quenching of APBA-dQDs for F(-) was enhanced. Moreover, the proposed methodology for the sensing of F(-) at EM 560nm in MC3T3-E1 osteoblastic cells was demonstrated and got a satisfactory results. The results indicate that the APBA-dQDs are promising candidates for intracellular in MC3T3-E1 osteoblastic cells. To the best of our knowledge, it was the first report of F(-) sensing by using the quenched fluorescence of APBA-dQDs in non-cancerous cells.
authors.affiliation: Institute of Endemic Disease of Jilin University, Changchun 130021, China; The Second Hospital Norman Bethune of Jilin University, Changchun 130041, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Hu
authors.affiliation: Institute of Endemic Disease of Jilin University, Changchun 130021, China.
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Zhao
authors.affiliation: Institute of Endemic Disease of Jilin University, Changchun 130021, China.
authors.forename: Xiu-Yun
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: The First Hospital Norman Bethune of Jilin University, Changchun 130021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Second Hospital Norman Bethune of Jilin University, Changchun 130041, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Duan
authors.affiliation: Institute of Endemic Disease of Jilin University, Changchun 130021, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Liu
authors.affiliation: Institute of Endemic Disease of Jilin University, Changchun 130021, China. Electronic address: jingling85619287@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: Qma@jlu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002460:Cell Line; D005459:Fluorides / Q000032:analysis* / Q000737:chemistry; D008345:Manganese / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts; D045663:Quantum Dots / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D018036:Selenium Compounds; D017967:Zinc Compounds; C028592:3-aminobenzeneboronic acid; D008345:Manganese; D013691:Tellurium; C044696:zinc selenide; D005459:Fluorides
keywords: F(–) sensing; Intracellular sensor; MC3T3-E1 osteoblastic cells; Quantum dots