title: Long-term low-level laser therapy promotes an increase in maximal oxygen uptake and exercise performance in a dose-dependent manner in Wistar rats.
abstract: The use of low-level laser therapy (LLLT) represents a new intervention modality that has been explored to enhance exercise performance. The aim of this study was to evaluate the influence of LLLT (GaAIAs-850 nm) at different doses on VO2max and on exercise performance in rats. Male Wistar rats were divided into three groups: "placebo" rats (P-LLLT, n = 10), rats at a dose of 0.315 J per treatment point of LLLT (8.7 J/cm(2)-LLLT, n = 10), and rats at a dose of 2.205 J per treatment point of LLLT (61.2 J/cm(2)-LLLT, n = 10). The LLLT was applied bilaterally at the biceps femoris, gluteus, lateral and medial gastrocnemius, iliopsoas, and adductor longus muscles. One spot in each muscle belly was applied, with a sum of 12 spots in each rat, once a day, for 10 days. All animals performed the maximal exercise test (ET) at a metabolic treadmill for rats, with simultaneous gas analysis. The distance covered was measured during ET, before and after the conclusion of the LLLT protocol. The data were compared by a repeated measures two-way ANOVA followed by the Student-Newman-Keuls post hoc tests (p < .05). The 61.2 J/cm(2)-LLLT group increased VO2basal (~40 %), VO2max (~24 %), VCO2max (~17 %), and distance covered (~34 %) after LLLT application on the skeletal muscle. No significant results were found comparing before and after conditions for the studied variables considering P-LLLT and 8.7 J/cm(2)-LLLT groups. The LLLT promoted in a dose-dependent manner an increase in oxygen consumption uptake and a performance increment of male Wistar rats.
authors.affiliation: Laboratory of Experimental Physiology, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
authors.forename: Júlia Luiza
authors.initials: JL
authors.lastname: Perini
authors.affiliation: Laboratory of Experimental Physiology, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.|Post-Graduation Program in Health Sciences, UFCSPA, Porto Alegre, RS, Brazil.
authors.forename: Vítor Scotta
authors.initials: VS
authors.lastname: Hentschke
authors.affiliation: Laboratory of Experimental Physiology, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.|Post-Graduation Program in Rehabilitation Sciences, UFCSPA, Porto Alegre, RS, Brazil.
authors.forename: Anelise
authors.initials: A
authors.lastname: Sonza
authors.affiliation: Laboratory of Experimental Physiology, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil. pdallago@ufcspa.edu.br.|Post-Graduation Program in Health Sciences, UFCSPA, Porto Alegre, RS, Brazil. pdallago@ufcspa.edu.br.|Post-Graduation Program in Rehabilitation Sciences, UFCSPA, Porto Alegre, RS, Brazil. pdallago@ufcspa.edu.br.|Physical Therapy Department, UFCSPA, Porto Alegre, RS, Brazil. pdallago@ufcspa.edu.br.|Universidade Federal de Ciências da Saúde de Porto Alegre, Sarmento Leite, 245, Porto Alegre, RS, 90050-170, Brazil. pdallago@ufcspa.edu.br.
authors.forename: Pedro
authors.initials: P
authors.lastname: Dal Lago
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D028022:Low-Level Light Therapy; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D010100:Oxygen / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010100:Oxygen
keywords: Exercise performance; Functional capacity; Maximal exercise test; Maximal oxygen consumption; Phototherapy; Skeletal muscle