title: Efficient extracellular expression of Bacillus deramificans pullulanase in Brevibacillus choshinensis.
abstract: In this study, the pullulanase gene from Bacillus deramificans was efficiently expressed in Brevibacillus choshinensis. The optimal medium for protein expression was determined through a combination of single-factor experiments and response surface methodology. The initial pH of the medium and the culture temperature were optimized. The pullulanase yield increased 10.8-fold through medium and condition optimization at the shake-flask level. From the results of these experiments, the dissolved oxygen level was optimized in a 3-L fermentor. Under these optimized conditions, the pullulanase activity and the specific pullulanase productivity reached 1005.8 U/mL and 110.5 × 10(3) U/g dry cell weight, respectively, with negligible intracellular expression. The Brevibacillus choshinensis expression system has proven to be valuable for the extracellular production of pullulanase.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.|School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.|School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
authors.forename: Xuguo
authors.initials: X
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China. jingwu@jiangnan.edu.cn.|School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China. jingwu@jiangnan.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D019149:Bioreactors; D058675:Brevibacillus / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D006026:Glycoside Hydrolases; C100181:pullulanase
keywords: Bacillus deramificans; Brevibacillus choshinensis; Fermentation optimization; Pullulanase; Response surface methodology