title: Paraclostridium benzoelyticumgen. nov., sp. nov., isolated from marine sediment and reclassification ofClostridium bifermentansasParaclostridium bifermentanscomb. nov. Proposal of a new genusPaeniclostridiumgen. nov. to accommodateClostridium sordelliiandClostridium ghonii.
abstract: Twenty-three rod-shaped, endospore-forming, Gram-stain-positive, obligately anaerobic bacteria were isolated from different marine sediment samples of Gujarat. All 23 strains shared 16S rRNA gene sequence similarity of ∼100 %. Strain JC272Twas designated the type strain and shared highest sequence similarity withClostridium bifermentansATCC 638T(99.8 %),Clostridium ghoniiJCM 1400T(98.0 %),Clostridium sordelliiATCC 9714T(97.9 %) and other members of the genusClostridium( < 96.4 %). C16 : 0, C18 : 0, C17 : 0, C16 : 1ω9cand iso-C16 : 0were the major (>5 %) fatty acids. Strain JC272Tcontained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified amino lipids. Genome-based analysis of average nucleotide identity (ANI) andin silicoDNA-DNA hybridization (DDH) of strain JC272TwithC. bifermentansATCC 638Tyielded values of 94.35 and 58.5 ± 2.8 %, respectively. The DNA G+C content of strain JC272Twas 28.3 mol%. Strain JC272Ttogether withC. bifermentanswere found to fall outsideClostridiumrRNA cluster I considered asClostridium senso stricto. Based on ANI value,in-silicoDDH, and distinct morphological and physiological differences from the previously described taxa, we suggest that strain JC272Trepresents a novel species of a new genus in the familyClostridiaceae, for which the nameParaclostridium benzoelyticumgen. nov., sp. nov. is proposed. The type strain is JC272T( = KCTC 15476T= LMG 28745T). It is also proposed to transferC. bifermentansto this new genus, asParaclostridium bifermentanscomb. nov. (type strain ATCC 638T= DSM 14991T= JCM 1386T). The genusPaeniclostridiumgen. nov. is proposed to accommodateC. sordelliiandC. ghoniiasPaeniclostridium sordelliicomb. nov. (type strain ATCC 9714T= LMG 15708T= JCM 3814T) andPaeniclostridium ghoniicomb. nov. (type strain ATCC 25757T= DSM 15049T= JCM 1400T).
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad,P.O. Central University, Hyderabad 500 046,India.
authors.forename: T S
authors.initials: TS
authors.lastname: Sasi Jyothsna
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad,P.O. Central University, Hyderabad 500 046,India.
authors.forename: L
authors.initials: L
authors.lastname: Tushar
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University Hyderabad,Kukatpally, Hyderabad 500 085,India.
authors.forename: Ch
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad,P.O. Central University, Hyderabad 500 046,India.
authors.forename: Ch V
authors.initials: CV
authors.lastname: Ramana