title: Protective effects of β-casofensin, a bioactive peptide from bovine β-casein, against indomethacin-induced intestinal lesions in rats.
abstract: METHODS
  β-casofensin, also known as peptide β-CN(94-123), is a milk bioactive peptide that modulates the intestinal barrier through its action on goblet cells. Here, we evaluated whether oral administration of β-casofensin can prevent indomethacin-induced injury of the jejunum in rats.
  
  
  RESULTS
  Rats received β-casofensin (0.01-100 μM) or tap water by daily gavage (4 μL/g) for eight days, then two subcutaneous injections of indomethacin (10 mg/kg, days 9 and 10) and were euthanized on day 12. In vitro, we investigated the effects of β-casofensin on the restitution of a wounded monolayer. Preventive administration of β-casofensin (100 μM) reduced intestinal macroscopic and microscopic damage induced by indomethacin. β-casofensin also prevented the depletion of goblet cells and increased myeloperoxidase activity, as well as tumor necrosis factor-ɑ (TNF-ɑ) expression and immunostaining of active caspase-3 in the jejunum of rats treated with indomethacin. In wound healing experiments, β-casofensin promoted epithelial restitution with no effect on cell proliferation. This effect was inhibited by pre-incubation with an anti-CC chemokine receptor 6 (CCR6) neutralizing antibody.
  
  
  CONCLUSIONS
  β-casofensin exerts protective effects in indomethacin-induced enteritis through preservation of goblet cells and improvement in wound healing. β-casofensin could therefore become vital in nutritional programs for the prevention of intestinal diseases.
authors.affiliation: INRA, UMR1397, Université Lyon 1, INSERM U1060, INSA-Lyon, CarMeN Laboratory, Villeurbanne, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Bessette
authors.affiliation: INRA, UMR1397, Université Lyon 1, INSERM U1060, INSA-Lyon, CarMeN Laboratory, Villeurbanne, France.
authors.forename: Bérengère
authors.initials: B
authors.lastname: Benoit
authors.affiliation: INRA, UMR1331 Toxalim, Group of Neuro-Gastroenterology and Nutrition, Toulouse, France.
authors.forename: Soraya
authors.initials: S
authors.lastname: Sekkal
authors.affiliation: INRA, UMR1397, Université Lyon 1, INSERM U1060, INSA-Lyon, CarMeN Laboratory, Villeurbanne, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Bruno
authors.affiliation: INRA, UMR1397, Université Lyon 1, INSERM U1060, INSA-Lyon, CarMeN Laboratory, Villeurbanne, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Estienne
authors.affiliation: INRA, UMR1253 Science et Technologie du Lait et de l'Œuf, AGROCAMPUS OUEST, Rennes, France.
authors.forename: Joelle
authors.initials: J
authors.lastname: Léonil
authors.affiliation: INRA, UMR1331 Toxalim, Group of Neuro-Gastroenterology and Nutrition, Toulouse, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Ferrier
authors.affiliation: INRA, UMR1331 Toxalim, Group of Neuro-Gastroenterology and Nutrition, Toulouse, France.
authors.forename: Vassilia
authors.initials: V
authors.lastname: Théodorou
authors.affiliation: INRA, UMR1397, Université Lyon 1, INSERM U1060, INSA-Lyon, CarMeN Laboratory, Villeurbanne, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Plaisancié
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002364:Caseins / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D004751:Enteritis / Q000139:chemically induced / Q000517:prevention & control; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D007213:Indomethacin / Q000009:adverse effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D007579:Jejunal Diseases / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002364:Caseins; D010446:Peptide Fragments; D020011:Protective Agents; C000613138:beta-casofensin; D007213:Indomethacin
keywords: Functional food; Inflammatory bowel disease; Intestinal protection; Milk bioactive peptide; Mucus