title: Proteomic and morphometric study of the in vitro interaction between Oncidium sphacelatum Lindl. (Orchidaceae) and Thanatephorus sp. RG26 (Ceratobasidiaceae).
abstract: Orchidaceae establish symbiotic relationships with fungi in the Rhizoctonia group, resulting in interactions beneficial to both organisms or in cell destruction in one of them (pathogenicity). Previous studies have focused mostly on terrestrial species with a few, preliminary studies, on epiphytes. To further our understanding of the molecular mechanisms involved in these symbioses, we evaluated the interaction between Oncidium sphacelatum Lindl. and the mycorrhizal fungus Thanatephorus sp. strain RG26 (isolated from a different orchid species) in vitro using morphometric and proteomic analyses. Evidence from the morphometric and microscopic analysis showed that the fungus promoted linear growth and differentiation of orchid protocorms during 98 days interaction. On day 63, protocorm development was evident, so we analyzed the physiological response of both organisms at that moment. Proteome results suggest that orchid development stimulated by the fungus apparently involves cell cycle proteins, purine recycling, ribosome biogenesis, energy metabolism, and secretion that were up-regulated in the orchid; whereas in the fungus, a high expression of proteins implicated in stress response, protein-protein interaction, and saccharides and protein biosynthesis were found in the symbiotic interaction. This is the first work reporting proteins differentially expressed in the epiphytic orchid-fungus interaction and will contribute to the search for molecular markers that will facilitate the study of this symbiosis in both wild orchids and those in danger of extinction.
authors.affiliation: El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, C.P. 30700, Tapachula, Chiapas, Mexico.
authors.forename: Mariana Yadira
authors.initials: MY
authors.lastname: López-Chávez
authors.affiliation: El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, C.P. 30700, Tapachula, Chiapas, Mexico. kguillen@ecosur.mx.
authors.forename: Karina
authors.identifier: http://orcid.org/0000-0001-5199-1156
authors.initials: K
authors.lastname: Guillén-Navarro
authors.affiliation: El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, C.P. 30700, Tapachula, Chiapas, Mexico.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Bertolini
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Col. Chamilpa, C.P. 62210, Cuernavaca, Morelos, Mexico.
authors.forename: Sergio
authors.initials: S
authors.lastname: Encarnación
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Col. Chamilpa, C.P. 62210, Cuernavaca, Morelos, Mexico.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Hernández-Ortiz
authors.affiliation: El Colegio de la Frontera Sur, Carretera Panamericana y Periférico Sur s/n, Barrio de María Auxiliadora, C.P. 29290, San Cristóbal de Las Casas, Chiapas, Mexico.
authors.forename: Irene
authors.initials: I
authors.lastname: Sánchez-Moreno
authors.affiliation: El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, C.P. 30700, Tapachula, Chiapas, Mexico.
authors.forename: Anne
authors.initials: A
authors.lastname: Damon
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000502:physiology*; D015415:Biomarkers; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D029595:Orchidaceae / Q000254:growth & development* / Q000382:microbiology* / Q000648:ultrastructure; D010802:Phylogeny; D040901:Proteomics; D013559:Symbiosis
chemical_list: D015415:Biomarkers
keywords: Ceratobasidium; Orchid mycorrhiza; Protocorm; Rhizoctonia; Symbiosis