title: Multiplex RT-PCR detection of H3N2 influenza A virus in dogs.
abstract: A multiplex RT-PCR (mRT-PCR) assay to detect H3N2 CIV genomic segments was developed as a rapid and cost-effective method. Its performance was evaluated with forty-six influenza A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously. The mRT-PCR has been successful in detecting the viral segments, indicating that it can improve the speed of diagnosis for H3N2 CIV and its reassortants.
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea.
authors.forename: EunJung
authors.initials: E
authors.lastname: Lee
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Kim
authors.affiliation: Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea. Electronic address: shinyk2009@korea.kr.
authors.forename: Yeun-Kyung
authors.initials: YK
authors.lastname: Shin
mesh_terms: D000818:Animals; D003362:Cost-Benefit Analysis; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology*; D004285:Dogs; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics*; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral
keywords: H3N2 CIV; Multiplex RT-PCR; Reassortants