title: Di-Sulfide Linked Polyethylenimine Coated Gold Nanoparticles as a Non-Viral Gene Delivery Agent in NIH-3T3 Mouse Embryonic Fibroblast.
abstract: Di-sulfide linked polyethylenimine coated gold nanoparticles (ssPEI-GNPs) of 20 nm size was prepared in order to deliver the genes to target site. DLS and TEM analysis demonstrated that the GNPs have average size of 13 nm in diameter. Upon coating the GNPs with ssPEI in the weight ratio of 1:3, the average hydrodynamic diameter of the ssPEI-GNPs was found to 19±1.14 nm and a zeta potential value 41±1.23 mV was observed. TEM analysis of ssPEI-GNPs demonstrated that the nanoparticles have spherical morphology. Thermogravemetric analysis of the prepared ssPEI-GNPs showed that the estimated composition of the ssPEI coated over the GNPs was approximately 5% (w/w). Gene expression capabilities of the nanoparticles were confirmed by fluorescent microscopy and luciferase assay, which demonstrated the transgene delivery capability of the ssPEI-GNPs. These results demonstrate that ssPEI-GNPs could be used as gene delivery agent.
authors.forename: Saji
authors.initials: S
authors.lastname: Uthaman
authors.forename: Myeong Ju
authors.initials: MJ
authors.lastname: Moon
authors.forename: Duhwan
authors.initials: D
authors.lastname: Lee
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Kim
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
mesh_terms: D000818:Animals; D004220:Disulfides / Q000737:chemistry*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D004220:Disulfides; D006046:Gold; D011094:Polyethyleneimine