title: Regulation of G0/G1 switch gene 2 (G0S2) expression in human adipose tissue.
abstract: The G0/G1 switch gene 2 (G0S2) protein attenuated adipose triglyceride lipase (ATGL) activity and decreased lipolysis in rodent and human adipocytes. We hypothesized that G0S2 mRNA expression in human adipose tissue is influenced by depot, adipocyte size, body weight and caloric intake. Adipose tissue samples were obtained during abdominal surgery and by needle biopsy before and 3 h after an extended glucose load in lean subjects. G0S2 mRNA was 7× higher expressed in mature human adipocytes compared to the stromavascular fraction. Cell size inversely correlated with G0S2 mRNA expression in both, subcutaneous and omental adipose depots. G0S2 mRNA expression was 75% higher in subcutaneous compared to omental adipose tissue. Obesity was associated with lower G0S2 mRNA expression in subcutaneous adipose tissue. Acute glucose ingestion after an overnight fast did not significantly increase G0S2 expression in subcutaneous adipose tissue. In conclusion, differences in G0S2 expression may explain depot-specific and obesity-associated differences in lipolysis on the molecular level.
authors.affiliation: a Institute of Clinical Pharmacology, Hannover Medical School , Hannover , Germany .
authors.forename: Alexander
authors.initials: A
authors.lastname: Skopp
authors.affiliation: a Institute of Clinical Pharmacology, Hannover Medical School , Hannover , Germany .
authors.forename: Marcus
authors.initials: M
authors.lastname: May
authors.affiliation: b Max-Delbrück Center for Molecular Medicine , Berlin , Germany .
authors.forename: Juergen
authors.initials: J
authors.lastname: Janke
authors.affiliation: c Department of Anatomy and Cell Biology , Martin Luther University Halle-Wittenberg , Halle (Saale) , Germany , and.
authors.forename: Heike
authors.initials: H
authors.lastname: Kielstein
authors.affiliation: d Surgical Department , Clementinenhaus , Hannover , Germany.
authors.forename: Ruth
authors.initials: R
authors.lastname: Wunder
authors.affiliation: d Surgical Department , Clementinenhaus , Hannover , Germany.
authors.forename: Ricarda
authors.initials: R
authors.lastname: Flade-Kuthe
authors.affiliation: d Surgical Department , Clementinenhaus , Hannover , Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kuthe
authors.affiliation: a Institute of Clinical Pharmacology, Hannover Medical School , Hannover , Germany .
authors.forename: Jens
authors.initials: J
authors.lastname: Jordan
authors.affiliation: a Institute of Clinical Pharmacology, Hannover Medical School , Hannover , Germany .
authors.forename: Stefan
authors.initials: S
authors.lastname: Engeli
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D018797:Cell Cycle Proteins / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000187:drug effects / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C508073:G0S2 protein, human; D012333:RNA, Messenger; D005947:Glucose
keywords: Adipose tissue; adipokines; lipid metabolism; lipolysis; obesity