title: MicroRNA-133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma.
abstract: Increasing evidence has suggested that microRNA-133b (miR-133b) is important in regulating the genesis of different types of cancer. However, the effects and the underlying mechanisms of miR-133b in the development of glioblastoma (GBM) remain largely unknown. The aim of the present study was to investigate the role of miR-133b in GBM and to determine the molecular mechanisms underlying its action. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression levels of miR-133b in 21 human GBM samples and 9 normal brain tissue samples. A wound healing assay, and Transwell migration and invasion assays were used to evaluate the effects of miR-133b on cell migration and invasion. Western blotting and a luciferase reporter assay were used to identify the target genes of miR-133b. It was found that miR-133b suppressed GBM cell migration and invasion, and matrix metalloproteinase 14 (MMP14) was identified as a direct target gene. In conclusion, miR-133b may suppress GBM migration and invasion through directly targeting MMP14, highlighting its potential as a novel agent for the treatment of GBM invasion.
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chang
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Lei
authors.affiliation: Department of Pathology, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Y U
authors.initials: YU
authors.lastname: Qin
authors.affiliation: Department of Pathology, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Xuexin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Guofu
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Tan
authors.affiliation: Department of Neurosurgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Su
keywords: glioblastoma; invasion; matrix metalloproteinase 14; microRNA-133b; migration