title: Aristolochia manshuriensis Kom ethyl acetate extract protects against high-fat diet-induced non-alcoholic steatohepatitis by regulating kinase phosphorylation in mouse.
abstract: Aristolochia manshuriensis Kom (AMK) is an herb used as a traditional medicine; however, it causes side effects such as nephrotoxicity and carcinogenicity. Nevertheless, AMK can be applied in specific ways medicinally, including via ingestion of low doses for short periods of time. Non-alcoholic steatohepatitis (NASH) induced the hepatocyte injury and inflammation. The protective effects of AMK against NASH are unclear; therefore, in this study, the protective effects of AMK ethyl acetate extract were investigated in a high-fat diet (HFD)-induced NASH model. We found decreased hepatic steatosis and inflammation, as well as increased levels of lipoproteins during AMK extract treatment. We also observed decreased hepatic lipid peroxidation and triglycerides, as well as suppressed hepatic expression of lipogenic genes in extract-treated livers. Treatment with extract decreased the activation of c-jun N-terminal kinase 1/2 (JNK1/2) and increased the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). These results demonstrate that the protective effect of the extract against HFD-induced NASH occurred via reductions in reactive oxygen species production, inflammation suppression, and apoptosis related to the suppression of JNK1/2 activation and increased ERK1/2 phosphorylation. Taken together, these results indicate that that ethyl acetate extract of AMK has potential therapeutic effects in the HFD-induced NASH mouse model.
authors.affiliation: Institute for Glycoscience College of Natural Sciences, Wonkwang University, Iksan 54538, Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Kwak
authors.affiliation: National Primate Research Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, Korea.
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: National Primate Research Center, Korea Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, Korea.
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
authors.affiliation: Institute for Glycoscience College of Natural Sciences, Wonkwang University, Iksan 54538, Korea.|Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 54538, Korea.
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D030022:Aristolochia / Q000737:chemistry*; D059305:Diet, High-Fat; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics* / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D000085:Acetates; D010936:Plant Extracts; C007650:ethyl acetate; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Aristolochia manshuriensis Kom; c-jun N-terminal kinase 1/2; extracellular signal-regulated kinase 1/2; inflammation; nonalcoholic steatohepatitis