title: [Expression of Chimeric Influenza Hemagglutinin Antigen (cH7/3) using the Baculovirus Expression System and Identification of its Biological Activities].
abstract: Hemagglutinin (HA) contains a head domain with a high degree of variability and a relatively conserved stem region. HA is the major viral antigen on the surface of the influenza virus. To define the biologic activities of chimeric HA bearing different head domains and stem regions or their potential use, a HA chimeric gene containing the head domain of the H7 subtype virus and stem region of the H3 subtype virus was modified and expressed using a baculovirus expression vector. Then, the secreted protein was purified and its biologic activities characterized. Approximately 1.4 mg/mL cH7/3 HA could be obtained, and its molecular weight was ≈ 70 kD. The trimer form of cH7/3 protein had hemagglutination activity and could be recognized by specific antibodies. The method described here can be used for further studies on the screening of HA stem-reactive antibodies or the development of vaccines with conserved epitopes.
authors.forename: Donghong
authors.initials: D
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Qin
authors.forename: Jinlei
authors.initials: J
authors.lastname: Guo
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Shuai
authors.initials: S
authors.lastname: Lu
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Shu
authors.forename: Jianfang
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006384:Hemagglutination; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology; D007251:Influenza, Human / Q000517:prevention & control / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines