title: Morphology engineering of bacteria for bio-production.
abstract: The concept of "morphology engineering" is proposed here. There are many genes involved in maintaining the bacterial shapes. The manipulations of these genes allow us to change the bacterial shapes from rods to fibers or to small spheres or large spheres. The advantages of morphology engineered bacteria for bio-production including accelerated growth, high cell density, simplification of downstream separation, enlarged space for more inclusion body accumulation and reduction on the cost of bio-production, have recently started to be exploited. So far only a few shape related genes have been manipulated for bioprocess benefits, many more genes are to be exploited for various cell morphologies. The limits of bacterial lengths and diameters may depend on how we manipulate relevant genes. Over time, these limits can be broken to enhance bioprocess competitiveness including improvements on the effectiveness of up- and downstream bioprocessing. Morphology engineering is just starting to show its promises.
authors.affiliation: MOE Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Xiao-Ran
authors.initials: XR
authors.lastname: Jiang
authors.affiliation: MOE Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China; Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China; Beijing Key Laboratory of Protein Therapeutics, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China. Electronic address: chengq@mail.tsinghua.edu.cn.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D057005:Bioengineering; D048430:Cell Shape / Q000235:genetics / Q000502:physiology; D005285:Fermentation; D054813:Polyhydroxyalkanoates / Q000378:metabolism; D058615:Synthetic Biology
chemical_list: D054813:Polyhydroxyalkanoates
keywords: Bioprocessing; Cell division; Downstream processing; Fermentation; Morphology; PHB; Synthetic biology; mreB; sulA