title: Long-term ex vivo and in vivo monitoring of tumor progression by using dual luciferases.
abstract: We propose a new concept of tumor progression monitoring using dual luciferases in living animals to reduce stress for small animals and the cost of luciferin. The secreted Cypridina luciferase (CLuc) was used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase was used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Thus, the new monitoring systems that use dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Hokkaido 062-8517, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Morita
authors.affiliation: Laboratory of Molecular and Functional Bio-Imaging, Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan.
authors.forename: Sanae
authors.initials: S
authors.lastname: Haga
authors.affiliation: DAILAB, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohmiya
authors.affiliation: Laboratory of Molecular and Functional Bio-Imaging, Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan. Electronic address: ozaki-m@med.hokudai.ac.jp.
authors.forename: Michitaka
authors.initials: M
authors.lastname: Ozaki
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003530:Cyprinidae / Q000235:genetics; D004195:Disease Models, Animal; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000032:analysis* / Q000235:genetics; D049408:Luminescent Agents / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000473:pathology*; D061848:Optical Imaging / Q000379:methods*
chemical_list: D049408:Luminescent Agents; C485195:luciferase, cypridina; D008156:Luciferases
keywords: Cypridina luciferase (CLuc); Dual reporter; Ex vivo real-time monitoring; In vivo real-time monitoring; Tumor progression