title: Design, Synthesis, Spectral Analysis, In Vitro Anticancer Evaluation and Molecular Docking Studies of Some Fluorescent 4-Amino-2, 3-Dimethyl-1-Phenyl-3-Pyrazolin-5-One, Ampyrone Derivatives.
abstract: The commenced work deals with the synthesis, characterization and evaluation of biological activities of 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one. The synthesis was done by the condensation of aromatic acid chlorides with 4-aminoantipyrine. The structures of synthesized derivatives were elucidated using IR, Mass,1H NMR and13C NMR spectroscopy, and their UV-Visible and fluorescence properties were studied. The compounds showed significant dual fluorescence. Molecular docking was used to understand the small molecule-receptor protein interaction. The derivatives were screened for their in vitro cytotoxic activity against the reference drug pazopanib on human cervical cancer cell line (SiHa) using MTT assay.
authors.affiliation: Department of Biotechnology, School of Health Science and Biotechnology, Karunya University, Coimbatore, Tamil Nadu, 641114, India. premnathdhanaraj@gmail.com.
authors.forename: D
authors.initials: D
authors.lastname: Premnath
authors.affiliation: Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore, Tamil Nadu, 641114, India.
authors.forename: Israel V M V
authors.initials: IV
authors.lastname: Enoch
authors.affiliation: Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore, Tamil Nadu, 641114, India.
authors.forename: P Mosae
authors.initials: PM
authors.lastname: Selvakumar
authors.affiliation: Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Indiraleka
authors.affiliation: Department of Biotechnology, School of Health Science and Biotechnology, Karunya University, Coimbatore, Tamil Nadu, 641114, India.
authors.forename: J Jannet
authors.initials: JJ
authors.lastname: Vennila
mesh_terms: D000675:Ampyrone / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007191:Indazoles; D009682:Magnetic Resonance Spectroscopy; D062105:Molecular Docking Simulation; D047069:Pyrazolones / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013449:Sulfonamides / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D007191:Indazoles; D047069:Pyrazolones; D011743:Pyrimidines; D013449:Sulfonamides; D000675:Ampyrone; C516667:pazopanib
keywords: 4-Aminoantipyrine; Cytotoxic activity; Fluorescence spectroscopy; Molecular docking