title: Pseudorabies virus glycoprotein gE triggers ERK1/2 phosphorylation and degradation of the pro-apoptotic protein Bim in epithelial cells.
abstract: ERK1/2 (Extracellular signal Regulated Kinase 1/2) signaling is a key cellular signaling axis controlling many cellular events, including cell survival. Activation of ERK 1/2 may trigger an anti-apoptotic response, and different viruses have been shown to benefit from this process. We have described recently that the viral glycoprotein gE mediates pseudorabies virus (PRV)-induced activation of ERK 1/2 in T lymphocytes. In the present study, we report that PRV gE-mediated ERK 1/2 phosphorylation also occurs in epithelial cells and that in these cells, gE-mediated ERK 1/2 signaling is associated with degradation of the pro-apoptotic protein Bim. Our results for the first time link the viral glycoprotein gE, an important alphaherpesvirus virulence factor, with the apoptotic signaling pathway.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Pontes
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Cliff
authors.initials: C
authors.lastname: Van Waesberghe
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Hans
authors.initials: H
authors.lastname: Nauwynck
authors.affiliation: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Belgium.
authors.forename: Bruno
authors.initials: B
authors.lastname: Verhasselt
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium. Electronic address: Herman.Favoreel@UGent.be.
authors.forename: Herman W
authors.initials: HW
authors.lastname: Favoreel
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D000072224:Bcl-2-Like Protein 11; D004847:Epithelial Cells / Q000821:virology*; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D054884:Host-Pathogen Interactions; D020935:MAP Kinase Signaling System; D008565:Membrane Proteins / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D059748:Proteolysis; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D000072224:Bcl-2-Like Protein 11; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
keywords: Bim; ERK1/2; Pseudorabies virus; gE