title: High frequency of multidrug-resistant Staphylococcus aureus with SCCmec type III and Spa types t037 and t631 isolated from burn patients in southwest of Iran.
abstract: Methicilin resistance Staphylococcus aureus (MRSA) infections are the major challenges in hospitals, especially in the burn units. The use of molecular typing methods is essential for tracking the spread of S. aureus infection and epidemiological investigations. The aim of this study was to find the profile of the spa types and also the prevalence of each SCCmec type of S. aureus strains in a central burn hospital in southwest of Iran. A total of 81 non-duplicate S. aureus were isolated from burn patients between April 2011 and February 2012. The susceptibility of the isolates against 13 different antibiotics was tested by disk agar diffusion (DAD) method. MRSA strains were identified by amplification of mecA gene. Multiplex-polymerase chain reaction (PCR) technique was used to determine the SCCmec types of MRSA strains and all the S. aureus isolates were typed by spa typing method. Detection of mecA gene showed that 70 (86.4%) of the isolates were MRSA. The highest rate of resistance was observed for penicillin (97.5%) and erythromycin (77.8%). None of the isolates were resistant to vancomycin. Sixty-seven of the 70 MRSA isolates harbored only SCCmec type III and three untypeable isolates. Five different spa types were detected. The most common spa types were t037 (42.5%) and t631 (34.5%) and were only found in MRSA isolates. Only SCCmec type III was found in burn patients which emphasizes the HA-MRSA origin of these strains. Only five different spa types identified in this study are in accordance with one SCCmec type which indicates that a limited number of bacterial colons are circulated in the burn unit in this hospital.
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Parhizgari
authors.affiliation: Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Seyed Sajjad
authors.initials: SS
authors.lastname: Khoramrooz
authors.affiliation: Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Seyed Ali Asghar
authors.initials: SA
authors.lastname: Malek Hosseini
authors.affiliation: Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Marashifard
authors.affiliation: Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Yazdanpanah
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
authors.affiliation: School of veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Gharibpour
authors.affiliation: Department of Microbiology, School of Medicine, Shahrood University of Medical Sciences, Shahrood, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mirzaii
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Darban-Sarokhalil
authors.affiliation: Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Moein
authors.affiliation: Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Naraki
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002056:Burns / Q000188:drug therapy / Q000382:microbiology; D003428:Cross Infection / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D005260:Female; D006801:Humans; D007492:Iran; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D010406:Penicillins / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C487789:mecA protein, Staphylococcus aureus; D004917:Erythromycin; D014640:Vancomycin
keywords: SCCmec type; Staphylococcus aureus; burn patient; spa type