title: A novel electrochemical immunosensor based on the rGO-TEPA-PTC-NH₂ and AuPt modified C₆₀ bimetallic nanoclusters for the detection of Vangl1, a potential biomarker for dysontogenesis.
abstract: The aberrant expression of Vangl1 is highly correlated with dysontogenesis, especially for neural tube defects. Therefore, the ultrasensitive detection of Vangl1 would provide a new approach for the specific early diagnostics in dysembryoplasia. However, no quantitative detection method is currently available. Herein, we describe the development of a new approach to fill this assay gap. We utilized C60-templated AuPt bimetallic nanoclusters for signal amplification because the promising C60 nanomaterial provides a large surface area for the in site reduction of bimetallic nanocomposites as well as excellent conductivity. To further amplify the electrochemical signal, reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) and a derivative of 3,4,9,10-perylenetetracarboxylicdianhydride (PTC-NH2) were selected for modification of the electrode to provide more amino groups for the immobilization of antibodies and to enhance the conductivity. The electrochemical signal was primarily derived from the catalysis of H2O2 by C60-AuPt. Chronoamperometry was applied to record the electrochemical signals. Under optimal conditions, the prepared immunosensor exhibited a wide linear range from 0.1 pg mL(-1) to 450 pg mL(-1) and a low detection limit of 0.03 pg mL(-1). Moreover, the proposed method exhibited good stability and recovery, suggesting its potential for use in clinical research.
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Qiutong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Rufei
authors.initials: R
authors.lastname: Gao
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Liuliu
authors.initials: L
authors.lastname: Gao
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Qingying
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Yuan
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: hejunlin_11@aliyun.com.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
mesh_terms: D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000097:blood*; D005030:Ethylenes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D008565:Membrane Proteins / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D005030:Ethylenes; D008565:Membrane Proteins; D010087:Oxides; D000644:Quaternary Ammonium Compounds; C488287:VANGL1 protein, human; C040648:pentamine; D006046:Gold; D006108:Graphite; C036216:ethylene; D006861:Hydrogen Peroxide
keywords: AuPt bimetallic; Dysontogenesis; Electrochemical catalysis; Reduced graphene oxide-tetraethylene; Vangl1