title: Chromatographic Evaluation and Characterization of Components of Gentian Root Extract Used as Food Additives.
abstract: Gentian root extract is used as a bitter food additive in Japan. We investigated the constituents of this extract to acquire the chemical data needed for standardized specifications. Fourteen known compounds were isolated in addition to a mixture of gentisin and isogentisin: anofinic acid, 2-methoxyanofinic acid, furan-2-carboxylic acid, 5-hydroxymethyl-2-furfural, 2,3-dihydroxybenzoic acid, isovitexin, gentiopicroside, loganic acid, sweroside, vanillic acid, gentisin 7-O-primeveroside, isogentisin 3-O-primeveroside, 6'-O-glucosylgentiopicroside, and swertiajaposide D. Moreover, a new compound, loganic acid 7-(2'-hydroxy-3'-O-β-D-glucopyranosyl)benzoate (1), was also isolated. HPLC was used to analyze gentiopicroside and amarogentin, defined as the main constituents of gentian root extract in the List of Existing Food Additives in Japan.
authors.affiliation: College of Pharmaceutical Sciences, Matsuyama University.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Amakura
authors.forename: Morio
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Sara
authors.initials: S
authors.lastname: Morimoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Atsuko
authors.initials: A
authors.lastname: Tada
authors.forename: Yusai
authors.initials: Y
authors.lastname: Ito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005503:Food Additives / Q000737:chemistry / Q000302:isolation & purification*; D030012:Gentiana / Q000737:chemistry*; D057888:Iridoid Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D005503:Food Additives; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; C012997:gentiopicroside; C102609:amarogentin