title: Complex of C60 Fullerene with Doxorubicin as a Promising Agent in Antitumor Therapy.
abstract: The main aim of this work was to evaluate the effect of doxorubicin in complex with C60 fullerene (C60 + Dox) on the growth and metastasis of Lewis lung carcinoma in mice and to perform a primary screening of the potential mechanisms of C60 + Dox complex action. We found that volume of tumor from mice treated with the C60 + Dox complex was 1.4 times less than that in control untreated animals. The number of metastatic foci in lungs of animals treated with C60 + Dox complex was two times less than that in control untreated animals. Western blot analysis of tumor lysates revealed a significant decrease in the level of heat-shock protein 70 in animals treated with C60 + Dox complex. Moreover, the treatment of tumor-bearing mice was accompanied by the increase of cytotoxic activity of immune cells. Thus, the potential mechanisms of antitumor effect of C60 + Dox complex include both its direct action on tumor cells by inducing cell death and increasing of stress sensitivity and an immunomodulating effect. The obtained results provide a scientific basis for further application of C60 + Dox nanocomplexes as treatment agents in cancer chemotherapy.
authors.affiliation: Taras Shevchenko National University of Kyiv, 64 Volodymyrska Str., 01601, Kyiv, Ukraine. psvit@bigmir.net.
authors.forename: Svitlana V
authors.initials: SV
authors.lastname: Prylutska
authors.affiliation: Taras Shevchenko National University of Kyiv, 64 Volodymyrska Str., 01601, Kyiv, Ukraine. realmed@i.com.ua.
authors.forename: Larysa M
authors.initials: LM
authors.lastname: Skivka
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU, Vasylkivska Str. 45, 03022, Kyiv, Ukraine. gennadij_d@mail.ru.
authors.forename: Gennadiy V
authors.initials: GV
authors.lastname: Didenko
authors.affiliation: Taras Shevchenko National University of Kyiv, 64 Volodymyrska Str., 01601, Kyiv, Ukraine. prylut@ukr.net.
authors.forename: Yuriy I
authors.initials: YI
authors.lastname: Prylutskyy
authors.affiliation: Belgorod State University, Pobedy Str.85, 308015, Belgorod, Russia. max_evstigneev@mail.ru.
authors.forename: Maxim P
authors.initials: MP
authors.lastname: Evstigneev
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU, Vasylkivska Str. 45, 03022, Kyiv, Ukraine. iris@onconet.kiev.ua.
authors.forename: Grygoriy P
authors.initials: GP
authors.lastname: Potebnya
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, 14-16 Drahomanov Str., 79005, Lviv, Ukraine. rpanchuk@ukr.net.
authors.forename: Rostyslav R
authors.initials: RR
authors.lastname: Panchuk
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, 14-16 Drahomanov Str., 79005, Lviv, Ukraine. stoika@cellbiol.lviv.ua.
authors.forename: Rostyslav S
authors.initials: RS
authors.lastname: Stoika
authors.affiliation: Technical University of Ilmenau, Institute of Chemistry and Biotechnology, 25 Weimarer Str., 98693, Ilmenau, Germany. uwe.ritter@tu-ilmenau.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Ritter
authors.affiliation: Technical University of Ilmenau, Institute of Chemistry and Biotechnology, 25 Weimarer Str., 98693, Ilmenau, Germany. peter.scharff@tu-ilmenau.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Scharff
keywords: Antimetastatic effect; Antitumor effect; C60 fullerene; Doxorubicin; Immune response