title: Co-expression of galectin-3 and CRIP-1 in endometrial cancer: prognostic value and patient survival.
abstract: Endometrial cancer is the sixth most common cancer in women. Galectin-3 (GAL-3) and CRIP-1 are multifunctional proteins which seem to be involved in many neoplasias. This study aims to point out correlations between clinicopathological findings and endometrial cancer patient survival to GAL-3 and CRIP-1 expression in order to enfold their diagnostic/prognostic potential. Tissues from 46 patients diagnosed with endometrial cancer were studied by immunohistochemistry, using monoclonal antibodies for GAL-3 and CRIP-1, and expression levels were correlated with clinicopathological findings and survival. Analysis was performed at single protein level or as co-expression. High expression of GAL-3 and CRIP-1 was independently associated with tumor depth and histological grade, respectively. Also, there was a significant correlation between high co-expression of the two proteins and the histological grade (aOR 2.66), the tumor depth (aOR 0.32) and the histological type (aOR 1.32), but not with the patients' age. Moreover, high expression of both proteins was observed in patients with shorter survival times. Interestingly, the co-expression of the two proteins exhibited some degree of monotony (Spearman's ρ = 0.768), indicating a common molecular pathway. This study provides evidence for a prognostic clinical potential of the combined study of GAL-3 and CRIP-1 in endometrial cancer. These factors are poorly studied in endometrium, and their role in the carcinogenetic process and on effective therapy awaits further elucidation.
authors.affiliation: Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Dragana, 68 100, Alexandroupolis, Greece. mlambro@med.duth.gr.
authors.forename: Maria
authors.identifier: http://orcid.org/0000-0001-8651-3680
authors.initials: M
authors.lastname: Lambropoulou
authors.affiliation: Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Dragana, 68 100, Alexandroupolis, Greece.
authors.forename: Theodora-Eleftheria
authors.initials: TE
authors.lastname: Deftereou
authors.affiliation: Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Dragana, 68 100, Alexandroupolis, Greece.
authors.forename: Sryridon
authors.initials: S
authors.lastname: Kynigopoulos
authors.affiliation: Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Dragana, 68 100, Alexandroupolis, Greece.
authors.forename: Anargyros
authors.initials: A
authors.lastname: Patsias
authors.affiliation: Laboratories of Biochemistry, School of Medicine, Democritus University of Thrace, 68 100, Alexandroupolis, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Anagnostopoulos
authors.affiliation: Private Radiodiagnostic Center of Alexandroupolis, Theagenio Anticancer Hospital, Thessaloniki, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Alexiadis
authors.affiliation: Laboratories of Medical Physics, School of Medicine, Democritus University of Thrace, 68 100, Alexandroupolis, Greece.
authors.forename: Athanasia
authors.initials: A
authors.lastname: Kotini
authors.affiliation: Laboratories of Department of Surgery, School of Medicine, Democritus University of Thrace, 68 100, Alexandroupolis, Greece.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Tsaroucha
authors.affiliation: Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Dragana, 68 100, Alexandroupolis, Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Nikolaidou
authors.affiliation: Pathology Laboratory, Theagenio Anticancer Hospital, Thessaloniki, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Kiziridou
authors.affiliation: Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Dragana, 68 100, Alexandroupolis, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Papadopoulos
authors.affiliation: Laboratories of Pharmacology, School of Medicine, Democritus University of Thrace, 68 100, Alexandroupolis, Greece.
authors.forename: Ekaterini
authors.initials: E
authors.lastname: Chatzaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D001798:Blood Proteins; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D005260:Female; D037502:Galectin 3 / Q000032:analysis / Q000096:biosynthesis*; D037161:Galectins; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D060588:LIM Domain Proteins / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; C486181:CRIP1 protein, human; D002352:Carrier Proteins; D037502:Galectin 3; D037161:Galectins; C428445:LGALS3 protein, human; D060588:LIM Domain Proteins
keywords: CRIP-1; Co-expression; Endometrial cancer; Galectin-3; Prognostic value