title: Interkinetic nuclear migration in the mouse embryonic ureteric epithelium: Possible implication for congenital anomalies of the kidney and urinary tract.
abstract: Interkinetic nuclear migration (INM) is a phenomenon in which progenitor cell nuclei migrate along the apico-basal axis of the pseudostratified epithelium, which is characterized by the presence of apical primary cilia, in synchrony with the cell cycle in a manner of apical mitosis. INM is suggested to regulate not only stem/progenitor cell proliferation/differentiation but also organ size and shape. INM has been reported in epithelia of both ectoderm and endoderm origin. We examined whether INM exists in the mesoderm-derived ureteric epithelium. At embryonic day (E) 11.5, E12.5 and E13.5, C57BL/6J mouse dams were injected with 5-bromo-2'-deoxyuridine (BrdU) and embryos were killed 1, 2, 4, 6, 8, 10 and 12 h later. We immunostained transverse sections of the ureter for BrdU, and measured the position of BrdU (+) nuclei in the ureteric epithelia along the apico-basal axis at each time point. We analyzed the distribution patterns of BrdU (+) nuclei in histograms using the multidimensional scaling. Changes in the nucleus distribution patterns suggested nucleus movement characteristic of INM in the ureteric epithelia, and the mode of INM varied throughout the ureter development. While apical primary cilia are related with INM by providing a centrosome for the apical mitosis, congenital anomalies of the kidney and urinary tract (CAKUT) include syndromes linked to primary ciliary dysfunction affecting epithelial tubular organs such as kidney, ureter, and brain. The present study showed that INM exists in the ureteric epithelium and suggests that INM may be related with the CAKUT etiology via primary ciliary protein function.
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Motoya
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ogawa
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nitta
authors.affiliation: Center for the Promotion of Project Research, Organization for Research, Shimane University, Matsue, Shimane, Japan.
authors.forename: Ashiq Mahmood
authors.initials: AM
authors.lastname: Rafiq
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Esrat
authors.initials: E
authors.lastname: Jahan
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Motohide
authors.initials: M
authors.lastname: Furuya
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Division of Anatomy and Cell Biology, Department of Anatomy, Shiga University of Medical Science, Otsu, Shiga, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Udagawa
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Shimane, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Otani
mesh_terms: D000015:Abnormalities, Multiple; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D007668:Kidney / Q000002:abnormalities / Q000196:embryology; D008297:Male; D051379:Mice; D008938:Mitosis; D014513:Ureter / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D014551:Urinary Tract / Q000002:abnormalities / Q000196:embryology
keywords: congenital anomalies of the kidney and urinary tract; embryo; interkinetic nuclear migration; mouse; ureter