title: Circulating microRNA-based screening tool for breast cancer.
abstract: Circulating microRNAs (miRNAs) are increasingly recognized as powerful biomarkers in several pathologies, including breast cancer. Here, their plasmatic levels were measured to be used as an alternative screening procedure to mammography for breast cancer diagnosis.A plasma miRNA profile was determined by RT-qPCR in a cohort of 378 women. A diagnostic model was designed based on the expression of 8 miRNAs measured first in a profiling cohort composed of 41 primary breast cancers and 45 controls, and further validated in diverse cohorts composed of 108 primary breast cancers, 88 controls, 35 breast cancers in remission, 31 metastatic breast cancers and 30 gynecologic tumors.A receiver operating characteristic curve derived from the 8-miRNA random forest based diagnostic tool exhibited an area under the curve of 0.81. The accuracy of the diagnostic tool remained unchanged considering age and tumor stage. The miRNA signature correctly identified patients with metastatic breast cancer. The use of the classification model on cohorts of patients with breast cancers in remission and with gynecologic cancers yielded prediction distributions similar to that of the control group.Using a multivariate supervised learning method and a set of 8 circulating miRNAs, we designed an accurate, minimally invasive screening tool for breast cancer.
authors.affiliation: University Hospital (CHU), Department of Medical Oncology, Liège, Belgium.|University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Frères
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Wenric
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Meriem
authors.initials: M
authors.lastname: Boukerroucha
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Corinne
authors.initials: C
authors.lastname: Fasquelle
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Thiry
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Molecular Angiogenesis, Liège, Belgium.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bovy
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Molecular Angiogenesis, Liège, Belgium.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Struman
authors.affiliation: University of Liège, GIGA-Research, Department of EE and CS, Liège, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Geurts
authors.affiliation: University Hospital (CHU), Department of Medical Oncology, Liège, Belgium.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Collignon
authors.affiliation: University Hospital (CHU), Department of Medical Oncology, Liège, Belgium.
authors.forename: Hélène
authors.initials: H
authors.lastname: Schroeder
authors.affiliation: University Hospital (CHU), Department of Gynecology, Liège, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Kridelka
authors.affiliation: University Hospital (CHU), Department of Senology, Liège, Belgium.
authors.forename: Eric
authors.initials: E
authors.lastname: Lifrange
authors.affiliation: Clinique Saint-Vincent (CHC), Department of Pathology, Liège, Belgium.
authors.forename: Véronique
authors.initials: V
authors.lastname: Jossa
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Vincent
authors.initials: V
authors.lastname: Bours
authors.affiliation: University of Liège, GIGA-Research, Laboratory of Human Genetics, Liège, Belgium.
authors.forename: Claire
authors.initials: C
authors.lastname: Josse
authors.affiliation: University Hospital (CHU), Department of Medical Oncology, Liège, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Jerusalem
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood / Q000235:genetics* / Q000556:secondary; D018275:Carcinoma, Lobular / Q000097:blood / Q000235:genetics* / Q000556:secondary; D016022:Case-Control Studies; D015331:Cohort Studies; D055088:Early Detection of Cancer; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human
keywords: biomarkers; breast cancer; circulating microRNAs; minimally invasive screening