title: Phospholipase A2 inhibitors modulate the effects of glutoxim and molixan on the intracellular Ca(2+) level in macrophages.
abstract: Using the fluorescent Ca(2+) probe Fura-2AM, the possible involvement of phospholipase A2, the key enzyme in the arachidonic acid cascade, in the effect of drugs glutoxim and molixan on the intracellular Ca(2+) concentration in macrophages was studied. It was shown for the first time that preincubation of macrophages with the classical phospholipase A2 inhibitor, 4-bromophenacyl bromide, as well as with glucocorticosteroids prednisolone and dexamethasone significantly inhibits Ca(2+) responses induced by glutoxim or molixan in macrophages. These results indicate the involvement of phospholipase A2 and arachidonic acid cascade in glutoximand molixan-induced signaling in macrophages.
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia. zk@bio.spbu.ru.
authors.forename: Z I
authors.initials: ZI
authors.lastname: Krutetskaya
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: L S
authors.initials: LS
authors.lastname: Milenina
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Naumova
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Antonov
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Nozdrachev
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D020013:Calcium Signaling; D002478:Cells, Cultured; D004338:Drug Combinations; D007155:Immunologic Factors / Q000494:pharmacology*; D007288:Inosine / Q000494:pharmacology* / Q000627:therapeutic use; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology* / Q000627:therapeutic use; D064801:Phospholipase A2 Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000098:Acetophenones; D004338:Drug Combinations; D007155:Immunologic Factors; D009842:Oligopeptides; D064801:Phospholipase A2 Inhibitors; C462699:glutoxim; C000611436:inosine glycyl-cysteinyl-glutamate disodium; D007288:Inosine; C006821:4-bromophenacyl bromide