title: Upregulation of p-Smad2 contributes to FAT10-induced oncogenic activities in glioma.
abstract: The human leukocyte antigen f-associated transcript 10 (FAT10) has a similar structure and function with ubiquitin, which efficiently mediate proteasome degradation in an ubiquitin-independent manner. FAT10 expression is upregulated in many tumor tissues and plays a vital role in cell cycle regulation and tumor genesis. However, its role in glioma has not been illuminated. The aim of this study was to evaluate the prognostic value of FAT10 and investigate its functional roles in glioma. The expression of FAT10 in glioma patient samples was examined using quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR), Western blotting and immunohistochemistry methods. Glioma cell lines with either FAT10 overexpression or knockdown were created. The effect of FAT10 on glioma cell migration and invasion was investigated using these cells. In the present study, we had shown that FAT10 was elevated significantly in glioma samples and correlated with tumor pathological grade. FAT10 high-expression glioma is associated with a poor clinical prognosis. Overexpression of FAT10 promoted proliferation, invasion, migration, and sphere formation of glioma cells, whereas downregulation of FAT10 had an opposite effect. Overexpression of FAT10 also promoted the growth of glioma cells in vivo. Moreover, FAT10 enhanced the phosphorylation of Smad2, which contributes to FAT10-induced oncogenic activities in glioma. In conclusion, these findings indicate that FAT10 is a critical regulator potential therapeutic target of glioma.
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Changcun
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Wan
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Junting
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Beijing Tian Tan Hospital, China National Clinical Research Center for Neurological Diseases, Capital Medical University, No.6 Tiantan Xili, Dongcheng District, Beijing, 100050, China. zhangliweitg@163.com.
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D005500:Follow-Up Studies; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010766:Phosphorylation; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051899:Smad2 Protein / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C497782:SMAD2 protein, human; D051899:Smad2 Protein; C109184:UBD protein, human; D014452:Ubiquitins
keywords: FAT10; Glioma; Proliferation; Smad2