title: SP, CGRP changes in pyridoxine induced neuropathic dogs with nerve growth factor gene therapy.
abstract: BACKGROUND
  Nerve growth factor (NGF) is known not only as a major factor for neuronal plasticity but also as a pain stimulator. Although there have been several trials with NGF for its application in the regeneration or protection of the nervous system, the pain induced by NGF remains a challenge to be overcome. In this study, the pain induced by NGF gene therapy was evaluated.
  
  
  RESULTS
  Vehicle or recombinant dog NGF plasmid was administered into the intrathecal space of dogs. Twenty-four hours after the vehicle or NGF plasmid inoculation, dogs were subcutaneously treated with 150 mg/kg pyridoxine every day for 7 days. For pain assessment, physical examination and electrophysiological recording were performed. Only in the vehicle-treated group, weight loss occurred, while NGF plasmid inoculation significantly improved this physical abnormalities. In the vehicle-treated group, electrophysiological recordings showed that H-reflex disappeared at 24 h after the last pyridoxine treatment. However, in the NGF plasmid inoculated group, the H-reflex were normal. In the results of immunohistochemistry, the NGF plasmid administration efficiently expressed in the dorsal root ganglia and significantly increased the pyridoxine-induced reduction of calcitonin gene-related peptide (CGRP) immunoreactive neurons, but not in substance P immunoreactive neurons, in the dorsal root ganglia.
  
  
  CONCLUSIONS
  Given these results, we reason that NGF gene therapy in pyridoxine induced neuropathic dogs does not induce neuropathic pain with this dosage, even with increasing the expression of CGRP.
authors.affiliation: Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine, Kangwon National University, Hyoja2-dong, Chuncheon-si, Gangwondo, 200-701, South Korea. jooyeon_02@naver.com.
authors.forename: Joo-Yeon
authors.initials: JY
authors.lastname: Kang
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Gwanak-ro 599, Gwanakgu, Seoul, 151-742, South Korea. yo4419@snu.ac.kr.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Yoo
authors.affiliation: Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Hyoja2-dong, Chuncheon-si, Gangwondo, 200-701, South Korea. tomotolky@kangwon.ac.kr.
authors.forename: Kwon-Young
authors.initials: KY
authors.lastname: Lee
authors.affiliation: Department of Neurology and Protein Metabolism Medical Research Center, College of Medicine, Seoul National University Hospital, 101 Daehakro, Chongno-ku, Seoul, 110-744, South Korea. imwooseok@gmail.com.
authors.forename: Wooseok
authors.initials: W
authors.lastname: Im
authors.affiliation: Department of Neurology and Protein Metabolism Medical Research Center, College of Medicine, Seoul National University Hospital, 101 Daehakro, Chongno-ku, Seoul, 110-744, South Korea. kimmanho@snu.ac.kr.
authors.forename: Manho
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Hyoja2-dong, Chuncheon-si, Gangwondo, 200-701, South Korea. jhchoi@kangwon.ac.kr.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Gwanak-ro 599, Gwanakgu, Seoul, 151-742, South Korea. hyyoun@snu.ac.kr.
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Youn
authors.affiliation: Department of Orthopedic Surgery, College of Medicine, Seoul National University Hospital, 101 Daehakro, Chongno-ku, Seoul, 110-744, South Korea. drjacobkim@gmail.com.
authors.forename: Sae Hoon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Gwanak-ro 599, Gwanakgu, Seoul, 151-742, South Korea. vetmed2@snu.ac.kr.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.affiliation: Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine, Kangwon National University, Hyoja2-dong, Chuncheon-si, Gangwondo, 200-701, South Korea. jychung77@gmail.com.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Chung
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D004285:Dogs; D005727:Ganglia, Spinal / Q000378:metabolism / Q000473:pathology; D015316:Genetic Therapy; D006181:H-Reflex; D006930:Hyperalgesia / Q000139:chemically induced; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D009437:Neuralgia / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010147:Pain Measurement; D011736:Pyridoxine; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D013373:Substance P / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D013373:Substance P; D020932:Nerve Growth Factor; D015740:Calcitonin Gene-Related Peptide; D011736:Pyridoxine