title: Highly oxygenated chromones from mangrove-derived endophytic fungus Rhytidhysteron rufulum.
abstract: Five highly oxygenated chromones, rhytidchromones A-E, were isolated from the culture broth of a mangrove-derived endophytic fungus, Rhytidhysteron rufulum, isolated from Thai Bruguiera gymnorrhiza. Their structures were determined by analysis of 1D and 2D NMR spectroscopic data. The structure of rhytidchromone A was further confirmed by single-crystal X-ray diffraction analysis. These compounds were evaluated for cytotoxicity against four cancer cell lines (MCF-7, Hep-G2, Kato-3 and CaSki). All compounds, except for rhytidchromone D, displayed cytotoxicity against Kato-3 cell lines with IC50 values ranging from 16.0 to 23.3μM, while rhytidchromones A and C were active against MCF-7 cells with IC50 values of 19.3 and 17.7μM, respectively.
authors.affiliation: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Supichar
authors.initials: S
authors.lastname: Chokpaiboon
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Siwattra
authors.initials: S
authors.lastname: Choodej
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Nattawut
authors.initials: N
authors.lastname: Boonyuen
authors.affiliation: Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under Royal Patronage, Pathumtani 13138, Thailand.
authors.forename: Thapong
authors.initials: T
authors.lastname: Teerawatananond
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: khanitha.p@chula.ac.th.
authors.forename: Khanitha
authors.initials: K
authors.lastname: Pudhom
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010100:Oxygen; D043982:Rhizophoraceae / Q000382:microbiology*; D013785:Thailand
chemical_list: D000970:Antineoplastic Agents; D002867:Chromones; C000610928:rhytidchromone A; D010100:Oxygen
keywords: Chromone; Cytotoxicity; Endophytic fungi; Rhytidhysteron rufulum