title: Protective effects of appropriate Zn(2+) levels against UVB radiation-induced damage in human lens epithelial cells in vitro.
abstract: As one of the crucial factors of cataract formation, ultraviolet B (UVB) can lead to apoptosis of human lens epithelial cells. Zinc, a cell-protective metal against various toxic compounds, plays an important role in protecting target cells from damage. Nevertheless, it is still unclear whether zinc exhibits protective effect on human lens epithelial cells (HLE B-3) against UVB-induced damage. In this study, we investigated the protective effect of zinc chloride (ZnCl2) on UVB-induced HLE B-3 cell damage, explored the molecular mechanisms using real-time cell electronic sensing system, flow cytometry, real-time quantitative PCR and enzyme-linked immunosorbent assay techniques. The results show that ZnCl2 is a potential inhibitor of UVB-induced HLE B-3 cell damage, and the underlying mechanisms are involved in decreasing the overproduction of reactive oxygen species and mitochondrial dysfunction, promoting intracellular calcium homeostasis recovery, and thus maintaining cell normal physiological functions. Taken together, our findings suggest that appropriate zinc levels have potential for protecting HLE B-3 cells against UVB-induced damage, and this finding may be clinically useful.
authors.affiliation: Shandong University of Traditional Chinese Medicine, 250002, Jinan, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, 250002, Jinan, China.
authors.forename: Dadong
authors.initials: D
authors.lastname: Guo
authors.affiliation: Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, 250002, Jinan, China.
authors.forename: Qiuxin
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Chemical and Molecular Technology, Qingdao University of Science and Technology, 266035, Qingdao, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.affiliation: Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, 250002, Jinan, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, 250002, Jinan, China. azuresky1999@163.com.|Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, 250002, Jinan, China. azuresky1999@163.com.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Bi
mesh_terms: D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002712:Chlorides / Q000008:administration & dosage; D006706:Homeostasis; D006801:Humans; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds / Q000008:administration & dosage
chemical_list: D002712:Chlorides; D017382:Reactive Oxygen Species; D017967:Zinc Compounds; C016837:zinc chloride; D015032:Zinc; D002118:Calcium
keywords: Calcium homeostasis; Plasma membrane calcium ATPase; Reactive oxygen species; Sarcoplasmic endoplasmic reticulum calcium ATPase; Ultraviolet B