title: [Progesterone Promotes Human Bone Marrow Mesenchymal Stem Cells to Synthesize Fibronectin via ERK Pathway].
abstract: OBJECTIVE
  To investigate whether the progesterone can promote fibronection (FN) synthesis by human bone marrow mesenchymal stem cells (MSCs) and to explore the potential underlying mechanism.
  
  
  METHODS
  The human bone marrow MSCs were cultured in a serum-free medium with progesterone for 72 hours, the MTT test was performed to observe the proliferation status and adhension ability of the treated cells. Western blot was used to detect the content of FN in MSDs with GAPDH as the internal reference, the phosphorylation of ERK1/2, as well as the FN content in MSC treated by PD98059, a specific inhibitor of ERK1/2.
  
  
  RESULTS
  The progesterone at a range of certain doses not effect on the proliferation of human bone marrow MSCs. Progesterone (25 µg/L) treatment enhanced the FN expression and adherent ability of marrow MSCs. Progesterone could induce prompt phosphorylation of ERK 1/2 and its promoting effects on FN synthesis was reversed by PD98059.
  
  
  CONCLUSIONS
  The progesterone can promote FN synthesis by human bone marrow MSCs via ERK 1/2 pathway, and it might be used to culture MSCs in serum-free medium.
authors.affiliation: Department of Clinical Laboratorial Examination, Hospital of Guangzhou Developmental District, Guangzhou 510730, Guangdong Province, China.
authors.forename: Zhen-Yong
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratorial Examination, Hospital of Guangzhou Developmental District, Guangzhou 510730, Guangdong Province, China.
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratorial Examination, Hospital of Guangzhou Developmental District, Guangzhou 510730, Guangdong Province, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratorial Examination, Hospital of Guangzhou Developmental District, Guangzhou 510730, Guangdong Province, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Bi
authors.affiliation: Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: guozk@bmi.ac.cn.
authors.forename: Zi-Kuan
authors.initials: ZK
authors.lastname: Guo
mesh_terms: D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005353:Fibronectins; D006412:Hematopoietic Stem Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells; D048052:Mitogen-Activated Protein Kinase 3; D010766:Phosphorylation; D011374:Progesterone
chemical_list: D005353:Fibronectins; D011374:Progesterone; D048052:Mitogen-Activated Protein Kinase 3