title: Distribution and activity levels of matrix metalloproteinase 2 and 9 in canine and feline osteosarcoma.
abstract: Overexpression of matrix metalloproteinases (MMPs) has been associated with increased tumor aggressiveness and metastasis dissemination. We investigated whether the contrasting metastatic behavior of feline and canine osteosarcoma is related to levels and activities of MMP2 and MMP9. Zymography and immunohistochemistry were used to determine expression levels of MMP2 and MMP9 in canine and feline osteosarcoma. Using immunohistochemistry, increased MMP9 levels were identified in most canine osteosarcomas, whereas cat samples more often displayed moderate levels. High levels of pro-MMP9, pro-MMP2, and active MMP2 were detected by gelatin zymography in both species, with significantly higher values for active MMP2 in canine osteosarcoma. These findings indicate that MMP2 is probably involved in canine and feline osteosarcoma and their expression and activity could be associated with the different metastatic behavior of canine and feline osteosarcoma.
authors.affiliation: Institute of Anatomy, Histology and Embryology (Gebhard, Walter), Institute of Pathology and Forensic Veterinary Medicine (Fuchs-Baumgartinger), VetCore Facility for Research (Razzazi-Fazeli), and Institute of Medical Biochemistry (Miller), University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Christiane
authors.initials: C
authors.lastname: Gebhard
authors.affiliation: Institute of Anatomy, Histology and Embryology (Gebhard, Walter), Institute of Pathology and Forensic Veterinary Medicine (Fuchs-Baumgartinger), VetCore Facility for Research (Razzazi-Fazeli), and Institute of Medical Biochemistry (Miller), University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fuchs-Baumgartinger
authors.affiliation: Institute of Anatomy, Histology and Embryology (Gebhard, Walter), Institute of Pathology and Forensic Veterinary Medicine (Fuchs-Baumgartinger), VetCore Facility for Research (Razzazi-Fazeli), and Institute of Medical Biochemistry (Miller), University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Razzazi-Fazeli
authors.affiliation: Institute of Anatomy, Histology and Embryology (Gebhard, Walter), Institute of Pathology and Forensic Veterinary Medicine (Fuchs-Baumgartinger), VetCore Facility for Research (Razzazi-Fazeli), and Institute of Medical Biochemistry (Miller), University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Miller
authors.affiliation: Institute of Anatomy, Histology and Embryology (Gebhard, Walter), Institute of Pathology and Forensic Veterinary Medicine (Fuchs-Baumgartinger), VetCore Facility for Research (Razzazi-Fazeli), and Institute of Medical Biochemistry (Miller), University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Walter
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary*; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary*
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9