title: Identification of a Hemagglutinin from Gallibacterium anatis.
abstract: Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149(T) secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis.
authors.affiliation: Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios # 1, Los Reyes Iztacala, 54090, Tlalnepantla, Estado de México, Mexico.
authors.forename: J F
authors.initials: JF
authors.lastname: Montes-García
authors.affiliation: Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios # 1, Los Reyes Iztacala, 54090, Tlalnepantla, Estado de México, Mexico. vacasergio@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Vaca
authors.affiliation: Centro de Investigaciones en Ciencias Microbiológicas, Instituto de ciencias, BUAP, Apdo. Postal 1622, 72560, Puebla, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Vazquez-Cruz
authors.affiliation: Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, 50200, Toluca, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Soriano-Vargas
authors.affiliation: CENID-Microbiología, Carr. Mexico-Toluca Km15.5, Cuajimalpa, 05110, Mexico, DF, Mexico.
authors.forename: F
authors.initials: F
authors.lastname: Aguilar-Romero
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland, 4067, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Blackall
authors.affiliation: Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios # 1, Los Reyes Iztacala, 54090, Tlalnepantla, Estado de México, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Negrete-Abascal
mesh_terms: D000818:Animals; D018441:Biofilms; D002241:Carbohydrates / Q000494:pharmacology; D002645:Chickens; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015661:Pasteurellaceae / Q000145:classification / Q000502:physiology* / Q000648:ultrastructure; D010802:Phylogeny
chemical_list: D002241:Carbohydrates; D006388:Hemagglutinins