title: Next Generation Sequencing Technologies: The Doorway to the Unexplored Genomics of Non-Model Plants.
abstract: Non-model plants i.e., the species which have one or all of the characters such as long life cycle, difficulty to grow in the laboratory or poor fecundity, have been schemed out of sequencing projects earlier, due to high running cost of Sanger sequencing. Consequently, the information about their genomics and key biological processes are inadequate. However, the advent of fast and cost effective next generation sequencing (NGS) platforms in the recent past has enabled the unearthing of certain characteristic gene structures unique to these species. It has also aided in gaining insight about mechanisms underlying processes of gene expression and secondary metabolism as well as facilitated development of genomic resources for diversity characterization, evolutionary analysis and marker assisted breeding even without prior availability of genomic sequence information. In this review we explore how different Next Gen Sequencing platforms, as well as recent advances in NGS based high throughput genotyping technologies are rewarding efforts on de-novo whole genome/transcriptome sequencing, development of genome wide sequence based markers resources for improvement of non-model crops that are less costly than phenotyping.
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource TechnologyPalampur, India; Department of Plant Science and Biotechnology, Imo State UniversityOwerri, Nigeria.
authors.forename: Chibuikem I N
authors.initials: CI
authors.lastname: Unamba
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, India.
authors.forename: Akshay
authors.initials: A
authors.lastname: Nag
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, India.
authors.forename: Ram K
authors.initials: RK
authors.lastname: Sharma
keywords: genomics; next generation sequencing; non-model; transcriptome; whole genome