title: Penicillin impregnation on oxygen plasma surface functionalized chitosan/Antheraea assama silk fibroin: Studies of antibacterial activity and antithrombogenic property.
abstract: Low temperature plasma can effectively tailor the surface properties of natural polymeric biomaterials according to the need for various biomedical applications. Non-mulberry silk, Antheraea assama silk fibroin (AASF) is a natural polymer having excellent biocompatibility and mechanical strength yet unlike mulberry silk, Bombyx mori silk fibroin, has drawn less interest in biomedical research. In the quest for developing as potential biomaterial, surface functionalization of plasma induced chitosan (Cs) grafted AASF ((AASF/O2-CS)g/O2) yarn is carried out using oxygen (O2) plasma. The (AASF/O2-CS)g/O2 yarn exhibits enhanced antithrombogenic property as well as antimicrobial activity against Gram positive (Bacillus subtilis) and Gram negative (Escherichia coli) bacteria as compared to AASF yarn. Moreover, impregnation of antibiotic drug (penicillin G sodium salt, PEN) on (AASF/O2-CS)g/O2 yarn further improves the observed properties. In-vitro hemolysis assay reveals that O2 plasma treatment and subsequent impregnation of PEN do not affect the hemocompatibility of AASF yarn. The present research findings demonstrate that plasma induced grafting of Cs followed by penicillin impregnation could significantly improve the potential applicability of AASF in the field of surgical research.
authors.affiliation: Department of Physics, Tezpur University, Napaam, Sonitpur 784028, Assam, India. Electronic address: arupjchoudhury@gmail.com.
authors.forename: Arup Jyoti
authors.initials: AJ
authors.lastname: Choudhury
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Garchuk, Guwahati 781035, Assam, India.
authors.forename: Dolly
authors.initials: D
authors.lastname: Gogoi
authors.affiliation: Drug Discovery Lab, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Raghuram
authors.initials: R
authors.lastname: Kandimalla
authors.affiliation: Drug Discovery Lab, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Sanjeeb
authors.initials: S
authors.lastname: Kalita
authors.affiliation: Molecular Biology and Microbial Biotechnology Lab, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Yogesh B
authors.initials: YB
authors.lastname: Chaudhari
authors.affiliation: Molecular Biology and Microbial Biotechnology Lab, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Mojibur R
authors.initials: MR
authors.lastname: Khan
authors.affiliation: Drug Discovery Lab, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Jibon
authors.initials: J
authors.lastname: Kotoky
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Garchuk, Guwahati 781035, Assam, India.
authors.forename: Joyanti
authors.initials: J
authors.lastname: Chutia
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D047011:Silk / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005343:Fibrinolytic Agents; D047011:Silk; D005349:Fibroins; D048271:Chitosan
keywords: Antheraea assama silk fibroin; Antibiotic drug; Antimicrobial; Antithrombogenic property; Chitosan; Oxygen plasma