title: Abundant and broad expression of transcription-induced chimeras and protein products in mammalian genomes.
abstract: The expression of transcription-induced chimeras (TICs) was underestimated due to strategic and logical reasons. In order to thoroughly examine TICs, systematic survey of TIC events was conducted in mammalian genomes using ESTs, followed by experimental validation using RT-PCR and real-time quantitative PCR (qPCR). The expression of ∼98% TIC events in at least one tissue or cell line from both mouse and human was verified. Besides, ∼40% TICs were broadly expressed, and ∼33% of TICs showed expression levels comparable to or higher than their upstream parental genes. We further identified putative chimeric proteins in public databases and validated two using Western blotting. GO analysis revealed that proteins resided in one multi-protein complex or functioning in metabolic or signaling pathway tended to produce fused products. Taken together, we have shown substantial evidence for the underestimated TIC events; and TICs could be a novel regulated way to further increases the proteome complexity in mammalian genomes. Possible regulation mechanisms and evolution of TICs were also discussed.
authors.affiliation: Department of Blood Transfusion, Tangdu Hospital, The Fourth Military Medical University, 710032, Xi'an, China.
authors.forename: Guanting
authors.initials: G
authors.lastname: Lu
authors.affiliation: Department of Clinical Oncology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Nanyang Institute of Technology, Henan, China.
authors.forename: Gangbin
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China. Electronic address: chenwh550@gmail.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Blood Transfusion, Tangdu Hospital, The Fourth Military Medical University, 710032, Xi'an, China. Electronic address: musj1963@163.com.
authors.forename: Shijie
authors.initials: S
authors.lastname: Mu
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation / Q000235:genetics*; D016678:Genome / Q000235:genetics*; D006801:Humans; D051379:Mice; D020543:Proteome / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D020543:Proteome
keywords: EST; Genome; TICS; qPCR