title: Generation of Skin Tissue Using Adipose Tissue-Derived Stem Cells.
abstract: BACKGROUND
  The aim of this study was to generate skin tissue using adipose tissue-derived mesenchymal stem cells.
  
  
  METHODS
  Thirty Wistar albino rats were used. A 2-cm-diameter full-thickness skin defect on the back of each rat was formed. A secondary wound healing model was constituted in group 1, fibrin matrix only was applied in group 2, a keratinocyte-coated fibrin matrix was applied in group 3, an adipose tissue-derived mesenchymal stem cell-seeded fibrin matrix was applied in group 4, and a keratinocyte-coated and adipose tissue-derived mesenchymal stem cell-seeded fibrin matrix was applied to the defects in group 5.
  
  
  RESULTS
  A similar form of wound healing, with contraction from the edges and ulceration at the center, was observed in groups 1, 2, and 3. The wound contraction was reduced in group 4, and the epidermis was creeping from the surrounding tissue but with some ulcerations in the central part of the wounds. In group 5, the defect area was almost totally epithelialized, with minimal wound contraction. By microscopic analysis, significant increases in the collagen volume ratios and vascular volume ratios were determined in groups 4 and 5. From the fluorescent micrographs, fibroblastic differentiation and extracellular matrix synthesis, endothelial differentiation of stem cells, and neoangiogenesis and epithelium derived from marked keratinocytes were observed in group 5.
  
  
  CONCLUSIONS
  Formation of the adipose tissue-derived mesenchymal stem cell-seeded and keratinocyte-coated autologous fibrin scaffold leads to significant skin replacement.
authors.affiliation: Adiyaman, Istanbul, Kirklareli, and Samsun, Turkey From the Department of Plastic, Reconstructive and Aesthetic Surgery, Government Hospital; the Department of Plastic, Reconstructive and Aesthetic Surgery, Bezmialem Vakif University; the Department of Plastic, Reconstructive and Aesthetic Surgery, Acibadem University; the Department of Haematolgoy, Acibadem University; the Department of Plastic, Reconstructive and Aesthetic Surgery, Government Hospital; and the Department of Histology, Ondokuz Mayis University.
authors.forename: Mustafa Aykut
authors.initials: MA
authors.lastname: Ozpur
authors.forename: Ethem
authors.initials: E
authors.lastname: Guneren
authors.forename: Halil Ibrahim
authors.initials: HI
authors.lastname: Canter
authors.forename: Mehmet Veli
authors.initials: MV
authors.lastname: Karaaltin
authors.forename: Ercument
authors.initials: E
authors.lastname: Ovali
authors.forename: Fatma Nilay
authors.initials: FN
authors.lastname: Yogun
authors.forename: Emre Gonenc
authors.initials: EG
authors.lastname: Baygol
authors.forename: Suleyman
authors.initials: S
authors.lastname: Kaplan
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology; D015603:Keratinocytes / Q000166:cytology / Q000637:transplantation; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D012680:Sensitivity and Specificity; D012867:Skin / Q000254:growth & development / Q000293:injuries*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous