title: Cdx2 represses Oct4 function via inducing its proteasome-dependent degradation in early porcine embryos.
abstract: Reciprocal repression of inner cell mass specific factor OCT4 and trophectoderm specific factor CDX2 promotes mouse first lineage segregation. Studies in mouse embryonic stem (ES) cells revealed that they bind to each other's regulatory regions to reciprocally suppress transcription, additionally they form protein complex for mutual antagonism. However, so far the molecular interaction of Oct4 and Cdx2 in other mammal's early embryo is not yet investigated. Here, over-expression of Cdx2 in early porcine embryo showed CDX2 represses Oct4 through neither the transcriptional repression nor forming repressive complex, but promoting OCT4 nuclear export and proteasomal degradation. The results showed novel molecular regulation of CDX2 on Oct4, and provided important clues for clarifying the mechanism of interaction between CDX2 and Oct4 in embryo of mammals other than mouse.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Gerelchimeg
authors.initials: G
authors.lastname: Bou
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Mingju
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Xue
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yanchang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Qingran
authors.initials: Q
authors.lastname: Kong
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: liu086@yahoo.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002478:Cells, Cultured; D018398:Homeodomain Proteins / Q000502:physiology*; D007976:Leupeptins / Q000494:pharmacology; D050810:Octamer Transcription Factors / Q000235:genetics / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine / Q000196:embryology*
chemical_list: D018398:Homeodomain Proteins; D007976:Leupeptins; D050810:Octamer Transcription Factors; D012333:RNA, Messenger; D046988:Proteasome Endopeptidase Complex; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde
keywords: Cdx2; Early embryo; Molecular interaction; Oct4; Pig; Proteasomal degradation