title: ZAP1-mediated modulation of triacylglycerol levels in yeast by transcriptional control of mitochondrial fatty acid biosynthesis.
abstract: The transcriptional activator Zap1p maintains zinc homeostasis in Saccharomyces cerevisiae. In this study, we examined the role of Zap1p in triacylglycerol (TAG) metabolism. The expression of ETR1 is reduced in zap1Δ. The altered expression of ETR1 results in reduced mitochondrial fatty acid biosynthesis and reduction in lipoic acid content in zap1Δ. The transcription factor Zap1 positively regulates ETR1 expression. Deletion of ETR1 also causes the accumulation of TAG, and the introduction of ETR1 in zap1Δ strain rescues the TAG level. These results demonstrated that the compromised mitochondrial fatty acid biosynthesis causes a reduction in lipoic acid and loss of mitochondrial function in zap1Δ. Functional mitochondria are required for the ATP production and defect in mitochondria slow down the process which may channeled carbon towards lipid biosynthesis and stored in the form of TAG.
authors.affiliation: Lipidomic Centre, Department of Lipid Science, CSIR-Central Food Technological Research Institute (CFTRI), Karnataka, Mysore, 570020, India.|Academy of Scientific & Innovative Research, CSIR-CFTRI, Mysore, India.
authors.forename: Neelima
authors.initials: N
authors.lastname: Singh
authors.affiliation: Lipidomic Centre, Department of Lipid Science, CSIR-Central Food Technological Research Institute (CFTRI), Karnataka, Mysore, 570020, India.|Academy of Scientific & Innovative Research, CSIR-CFTRI, Mysore, India.
authors.forename: Kamlesh Kumar
authors.initials: KK
authors.lastname: Yadav
authors.affiliation: Lipidomic Centre, Department of Lipid Science, CSIR-Central Food Technological Research Institute (CFTRI), Karnataka, Mysore, 570020, India.|Academy of Scientific & Innovative Research, CSIR-CFTRI, Mysore, India.
authors.forename: Ram
authors.initials: R
authors.lastname: Rajasekharan
mesh_terms: D001665:Binding Sites; D053898:Biosynthetic Pathways; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D050745:Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific) / Q000235:genetics; D005227:Fatty Acids / Q000096:biosynthesis*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014280:Triglycerides / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014280:Triglycerides; C103264:ZAP1 protein, S cerevisiae; D002244:Carbon; D050745:Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific); C541904:Etr1 protein, S cerevisiae; D015032:Zinc