title: Stem Cell-Associated Marker Expression in Canine Hair Follicles.
abstract: Functional hair follicle (HF) stem cells (SCs) are crucial to maintain the constant recurring growth of hair. In mice and humans, SC subpopulations with different biomarker expression profiles have been identified in discrete anatomic compartments of the HF. The rare studies investigating canine HF SCs have shown similarities in biomarker expression profiles to that of mouse and human SCs. The aim of our study was to broaden the current repertoire of SC-associated markers and their expression patterns in the dog. We combined analyses on the expression levels of CD34, K15, Sox9, CD200, Nestin, LGR5 and LGR6 in canine skin using RT-qPCR, the corresponding proteins in dog skin lysates, and their expression patterns in canine HFs using immunohistochemistry. Using validated antibodies, we were able to define the location of CD34, Sox9, Keratin15, LGR5 and Nestin in canine HFs and confirm that all tested biomarkers are expressed in canine skin. Our results show similarities between the expression profile of canine, human and mouse HF SC markers. This repertoire of biomarkers will allow us to conduct functional studies and investigate alterations in the canine SC compartment of different diseases, like alopecia or skin cancer with the possibility to extend relevant findings to human patients.
authors.affiliation: Institute of Animal Pathology, Dermfocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (NMG, BSS, AG, EJM, MMW, DJW)
authors.forename: Nora M
authors.initials: NM
authors.lastname: Gerhards
authors.affiliation: Institute of Animal Pathology, Dermfocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (NMG, BSS, AG, EJM, MMW, DJW)|Molecular Dermatology and Stem Cell Research, Institute of Animal Pathology and DermFocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (BSS, AG, EJM)
authors.forename: Beyza S
authors.initials: BS
authors.lastname: Sayar
authors.affiliation: Center for Fish and Wildlife Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland (FCO)
authors.forename: Francesco C
authors.initials: FC
authors.lastname: Origgi
authors.affiliation: Institute of Animal Pathology, Dermfocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (NMG, BSS, AG, EJM, MMW, DJW)|Molecular Dermatology and Stem Cell Research, Institute of Animal Pathology and DermFocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (BSS, AG, EJM)
authors.forename: Arnaud
authors.initials: A
authors.lastname: Galichet
authors.affiliation: Institute of Animal Pathology, Dermfocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (NMG, BSS, AG, EJM, MMW, DJW)|Molecular Dermatology and Stem Cell Research, Institute of Animal Pathology and DermFocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (BSS, AG, EJM)|Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland (EJM)
authors.forename: Eliane J
authors.initials: EJ
authors.lastname: Müller
authors.affiliation: Institute of Animal Pathology, Dermfocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (NMG, BSS, AG, EJM, MMW, DJW)
authors.forename: Monika M
authors.initials: MM
authors.lastname: Welle
authors.affiliation: Institute of Animal Pathology, Dermfocus, Vetsuisse Faculty, University of Bern, Bern, Switzerland (NMG, BSS, AG, EJM, MMW, DJW)
authors.forename: Dominique J
authors.initials: DJ
authors.lastname: Wiener
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D015415:Biomarkers / Q000032:analysis*; D004285:Dogs; D018859:Hair Follicle / Q000166:cytology*; D007150:Immunohistochemistry; D053569:Keratin-15 / Q000032:analysis / Q000235:genetics; D064231:Nestin / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000235:genetics; D055758:SOX9 Transcription Factor / Q000032:analysis / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D053569:Keratin-15; D064231:Nestin; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D043562:Receptors, G-Protein-Coupled; D055758:SOX9 Transcription Factor; C042739:antigens, CD200
keywords: Stem cells; canine; hair follicle; immunohistochemistry