title: Specific Metabolites in a Phaeodactylum tricornutum Strain Isolated from Western Norwegian Fjord Water.
abstract: We have searched for special characteristics in growth, protein expression, fatty acids and volatile organic compounds (VOCs) in a local Phaeodactylum tricornutum Bohlin strain (Bergen Marine Biobank), by comparing it with a common accession strain (CCAP). Differences in growth and expressed proteins were detected between the BMB strain and the CCAP strain, and the BMB strain reached the highest cell densities under the given growth conditions. Fatty acid (FA) analyses showed highest relative eicosapentaenoic acid (EPA) levels in the exponential phase (25.73% and 28.31%), and highest levels of palmitoleic acid (16:1 n-7) in the stationary phase (46.36% and 43.66%) in the BMB and CCAP strain, respectively. The most striking finding of the VOCs analyses was the relatively high levels of ectocarpene, 6-((1E)-butenyl)-1,4-cycloheptadiene, hormosirene, and desmarestene and structurally related compounds, which were exclusively detected in the BMB strain. Many of the VOCs detected in the CCAP and, in particular, in the BMB strain have been reported as antimicrobial agents. We suggest that the array of pheromones and antimicrobial substances could be part of an allelopathic strategy of the BMB strain, dominated by oval cells, thus reflecting the benthic life stage of this morphological form. These findings show the potential for bioactive metabolites in the BMB strain.
authors.affiliation: Bergen Marine Biobank, Department of Biology, University of Bergen, Thormøhlensgate 53A/B, N-5020 Bergen, Norway. siv.prestegard@uni.no.|Applied Biotechnology, Uni Research Environment, Thormøhlensgate 49B, N-5006 Bergen, Norway. siv.prestegard@uni.no.
authors.forename: Siv Kristin
authors.initials: SK
authors.lastname: Prestegard
authors.affiliation: Department of Biology, University of Bergen, Thormøhlensgate 53AB, N-5020 Bergen, Norway. svein.erga@uib.no.
authors.forename: Svein Rune
authors.initials: SR
authors.lastname: Erga
authors.affiliation: Department of Biology, University of Bergen, Thormøhlensgate 53AB, N-5020 Bergen, Norway. pia.steinrucken@uib.no.
authors.forename: Pia
authors.initials: P
authors.lastname: Steinrücken
authors.affiliation: Department of Chemistry, University of Bergen, Allégaten 42, N-5020 Bergen, Norway. svein.mjos@uib.no.
authors.forename: Svein Are
authors.initials: SA
authors.lastname: Mjøs
authors.affiliation: Department of Biology, University of Bergen, Thormøhlensgate 53AB, N-5020 Bergen, Norway. gjert.knutsen@uib.no.
authors.forename: Gjert
authors.initials: G
authors.lastname: Knutsen
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway. jens.rohloff@ntnu.no.
authors.forename: Jens
authors.initials: J
authors.lastname: Rohloff
mesh_terms: D017377:Diatoms / Q000737:chemistry* / Q000378:metabolism; D063366:Estuaries; D006801:Humans; D009664:Norway; D012623:Seawater; D055549:Volatile Organic Compounds / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D055549:Volatile Organic Compounds
keywords: Phaeodactylum tricornutum; ectocarpene; eicosapentaenoic acid (EPA); fatty acids; hormosirene; two-dimensional gel electrophoresis (2DGE); volatile organic compounds (VOCs)