title: Contribution of α-smooth muscle actin and extracellular matrix to the in vitro reorganization of cardiomyocyte contractile system.
abstract: Cardiomyocytes in culture undergo reversible rearrangement of their contractile apparatus with the conversion of typical myofibrils into the structures of non-muscle type and the loss of contractility. Along with these transformations, the cardiomyocytes gain the capacity to synthesize extracellular matrix. Here we show that during cultivation of rat neonatal cardiomyocytes, the inherent α-cardiac actin isoform is transiently replaced by α-smooth-muscle actin, whose expression is accompanied by transformation of myofibrils into stress-fiber-like structures. The following down-regulation of α-smooth muscle actin parallels restoration of myofibrillar system and correlates with the accumulation of extracellular collagen and laminin, initially missing from the cardiomyocytes culture.
authors.affiliation: Department of Cell Culture, Institute of Cytology RAS, Saint-Petersburg, 194064, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Bildyug
authors.affiliation: Department of Cell Culture, Institute of Cytology RAS, Saint-Petersburg, 194064, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Bozhokina
authors.affiliation: Department of Cell Culture, Institute of Cytology RAS, Saint-Petersburg, 194064, Russia.
authors.forename: Sofia
authors.initials: S
authors.lastname: Khaitlina
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009119:Muscle Contraction; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats
chemical_list: D000199:Actins; D003094:Collagen; D007797:Laminin; D020033:Protein Isoforms; C498862:Acta2 protein, rat
keywords: cardiomyocytes; cell culture; collagen; contractile apparatus; laminin; α-smooth muscle actin