title: An investigation into the association between HLA-G 14 bp insertion/deletion polymorphism and multiple sclerosis susceptibility.
abstract: Human Leukocyte Antigen G (HLA-G) gene polymorphism and expression rate have recently been suggested to have a potential role in susceptibility to Multiple Sclerosis (MS), a chronic inflammatory demyelinating and neurodegenerative disease of the central nervous system with unknown etiology. The aim of this study was to investigate the association of the frequency of HLA-G gene 14 bp insertion/deletion polymorphism and its plasma level with MS susceptibility. In this study, the HLA-G gene from 212 patients and 210 healthy individuals was amplified using real time PCR and screened for the 14 bp insertion/deletion polymorphism. In addition, HLA-G plasma levels of the patients were measured and compared to normal controls by ELISA method. Our results revealed that 14 bp insertion in HLA-G could result in lower plasma HLA-G level of the subjects, regardless of their health status and vice versa. Additionally, significant correlation of HLA-G genotype and its plasma level with MS susceptibility was observed. In conclusion, not only HLA-G 14 bp insertion/deletion polymorphism could be associated with expression rate of the HLA-G gene and its plasma level, but also could be considered as a risk factor for susceptibility to MS in our study population.
authors.affiliation: Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
authors.forename: Nabiallah
authors.initials: N
authors.lastname: Mohammadi
authors.affiliation: Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran. Electronic address: adib@med.mui.ac.ir.
authors.forename: Minoo
authors.initials: M
authors.lastname: Adib
authors.affiliation: Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Alsahebfosoul
authors.affiliation: Department of Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kazemi
authors.affiliation: Neurology Department, Faculty of Medicine, Isfahan University of Medical Sciences, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Etemadifar
mesh_terms: D000328:Adult; D005260:Female; D056726:Genetic Association Studies / Q000379:methods; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D059951:HLA-G Antigens / Q000235:genetics*; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D008297:Male; D009103:Multiple Sclerosis / Q000175:diagnosis* / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D055815:Young Adult
chemical_list: D059951:HLA-G Antigens
keywords: HLA-G; Insertion/deletion polymorphism; MS; Real time PCR.HRM