title: [RH Factor and Clinical Transfusion Effectiveness for β-Thalassemia Children with Long-Term Blood Transfusion].
abstract: OBJECTIVE
  To investigate the irregular antibody production and its relationship with Rh factor genotypes and the loci of thalassemia gene mutations for the β-thalassemic children with long-term transfusion, so as provide experimental basis for clinical safe and effective transfusions for thalassemic children.
  
  
  METHODS
  The peripheral blood from 246 children with β-thalassemia was collected in our hospital; the extraction of genomic DNA and Rh factor (C/c, E/e) genotypes were assayed by PCR-SSP method, the irregular antibodies were screened and identified by serological method, the genotypes for thalassemia and gene mutations were analysed by PCR-RD method.
  
  
  RESULTS
  The genotypes of Rh factors classified by PCR- SSP in the 246 cases of β-thalassemia children were as follws: Ce/Ce (143/246, 58.1%), CE/ce (59/246, 24%), cE/cE (14/24, 5.7%), Ce/ce (12/246, 4.9%); The positive rate of irregular antibody was 7.7% (19/246), including anti-E (7/19), anti-c (5/19), anti-C (2/19), anti-E and anti-c (2/19), anti-e (1/19), anti-D (2/19); Of the 19 cases with positive irregular antibody, the genotypings of Rh factor were: Ce/Ce (11/19), CE/ce (2/19), cE/cE (2/19), Ce/ce (2/19), cE/ce (2/19); the gene mutations location of thalassemia for 19 cases with positive irregular antibody: CD41-42M (13/19), CD71-72M (2/19), IVS-II-654M (3/19), -28M (1/19).
  
  
  CONCLUSIONS
  The irregular antibody production for β-thalassemic children with long-term transfusion may have some relevance with Rh factor genotypes and thalassemia genetic mutations. This study possesses a certain significance for effective prevention of RBC alloimmune response of β-thalassemia children and improvement of efficacy and safety of clinical transfasion blood.
authors.affiliation: Department of Blood Transfusion, The Second People's Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China.
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Wu
authors.affiliation: Institute of Evidence Identification of Chongqing Yuzhong District Public Security Bureau, Chongqing 400038, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Shenzhen Academy of Inspection and Quarantine, Shenzhen 518045, Guangdong Province, China.
authors.forename: Jian-An
authors.initials: JA
authors.lastname: He
authors.affiliation: Shenzhen Academy of Inspection and Quarantine, Shenzhen 518045, Guangdong Province, China. E-mail: wanhood@163.com.
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Gu
authors.affiliation: Department of Blood Transfusion, The Second People's Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China.
authors.forename: Xing-Tang
authors.initials: XT
authors.lastname: Dang
authors.affiliation: Department of Blood Transfusion, The Second People's Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Blood Transfusion, The Second People's Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China. E-mail: cpshaos@hotmail.com.
authors.forename: Chao-Peng
authors.initials: CP
authors.lastname: Shao
mesh_terms: D001789:Blood Group Antigens; D001803:Blood Transfusion; D002648:Child; D005838:Genotype; D006648:Histocompatibility; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin; D017086:beta-Thalassemia
chemical_list: D001789:Blood Group Antigens; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin