title: Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties.
abstract: In this study, we report the synthesis of copper oxide nanoparticles (CuO NPs) using a medicinal plant (Matricaria chamomilla) flower extract as both reducing and capping agent and investigate their antioxidant activity and interaction with plasmid DNA (pBR322).The CuO NPs were characterized using Uv-Vis spectroscopy, FT-IR (Fourier transform infrared spectroscopy), DLS (dynamic light scattering), XRD (X-ray diffraction), EDX (energy-dispersive X-ray) spectroscopy and SEM (scanning electron microscopy). The CuO NPs exhibited nearly mono-distributed and spherical shapes with diameters of 140 nm size. UV-Vis absorption spectrum of CuO NPs gave a broad peak around 285 and 320 nm. The existence of functional groups on the surface of CuO NPs was characterized with FT-IR analysis. XRD pattern showed that the NPs are in the form of a face-centered cubic crystal. Zeta potential value was measured as -20 mV due to the presence of negatively charged functional groups in plant extract. Additionally, we demonstrated concentration-dependent antioxidant activity of CuO NPs and their interaction with plasmid DNA. We assumed that the CuO NPs both cleave and break DNA double helix structure.
authors.affiliation: Erciyes University, Science Faculty, Biology Department, Kayseri 38039, Kayseri, Turkey. Electronic address: fduman@erciyes.edu.tr.
authors.forename: Fatih
authors.initials: F
authors.lastname: Duman
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039, Kayseri, Turkey; Erciyes University, Nanotechnology Research Center, 38039, Kayseri, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ocsoy
authors.affiliation: Erciyes University, Science Faculty, Biology Department, Kayseri 38039, Kayseri, Turkey.
authors.forename: Fatma Ozturk
authors.initials: FO
authors.lastname: Kup
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D020843:Chamomile / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D053837:DNA Cleavage / Q000187:drug effects; D035264:Flowers / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D003300:Copper; C000520:cuprous oxide
keywords: Characterization; CuO NPs; Green synthesis; Matricaria chamomilla flower extract; Plasmid DNA