title: [Effect of RYBP Gene Silencing on Sensitivity of HL-60 Cells to Chemotherapeutic Drugs].
abstract: OBJECTIVE
  To investigate the effect of RYBP gene on sensitivity of HL-60 cells to chemotherapy drugs by using RNA interference.
  
  
  METHODS
  Plasmid expressing RYBP specific shRNA was constructed and then was used to establish the RYBP knockdown stable HL-60 cell line. Q-PCR and Western blot were used to confirm the efficacy of RYBP gene silencing at mRNA and protein level respectively; then the DNA ladder and Annexin V labeled flow cytometry were used to detect cell apoptosis; CCK-8 was used detect the sensitivity of HL-60 cells to the chemotherapeutic drug cytarabine or daunorubicin.
  
  
  RESULTS
  The lentiviral-RYBP-shRNA vector was succesfully and effectively inhibit the expression of RYBP at mRNA and protein in HL-60 cells. It was found that without chemotherapy drug treatment the apoptosis rate of RYBP shRNA group was lower than that of the empty vector control group (NC group). When treated with cytarabine, the apoptosis rate and inhibitive rate of RYBP shRNA group were lower than those of NC group. Besides, when treated with daunorubicin, the apoptosis rate of RYBP shRNA group was lower than that of NC group, while the inhibitive rate had no significant difference.
  
  
  CONCLUSIONS
  RYBP gene silencing can inhibitive the apoptosis of HL-60 cells and significantly reduce the sensitivity to cytarabine, but this gene silencing can't affect the sensitivity to daunorubicin.
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zeng
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China. E-mail: drwangshq@medmail.corn. cn.
authors.forename: Shun-Qing
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Deng
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ying
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Chen
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Xu
authors.affiliation: Department of Hematology, Guangzhou Municipal First People's Hospital, Affiliated to Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Lin
mesh_terms: D017209:Apoptosis; D005822:Genetic Vectors; D018922:HL-60 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D016086:Lentivirus; D010957:Plasmids; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012097:Repressor Proteins
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C434957:RYBP protein, human; D012097:Repressor Proteins