title: Protein kinase profiling assays: a technology review.
abstract: Protein kinases have become one of the most intensively pursued classes of drug targets for many diseases such as cancers and inflammatory diseases. Kinase profiling work seeks to understand general selectivity trends of lead compounds across the kinome, which help with target selection, compound prioritization, and potential implications in toxicity. Under the current drug discovery process, screening of compounds against comprehensive panels of kinases and their mutants has become the standard approach. Many screening assays and technologies which are compatible for high-throughput screening (HTS) against kinases have been extensively pursued and developed.
authors.affiliation: Reaction Biology Corp., One Great Valley Parkway, Suite 2, Malvern, PA 19355, USA. Electronic address: yuren.wang@reactionbiology.com.
authors.forename: Yuren
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Reaction Biology Corp., One Great Valley Parkway, Suite 2, Malvern, PA 19355, USA.
authors.forename: Haiching
authors.initials: H
authors.lastname: Ma
mesh_terms: D055808:Drug Discovery / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: D047428:Protein Kinase Inhibitors; D011494:Protein Kinases