title: Next-generation sequencing yields the complete mitochondrial genome of the mummichog, Fundulus heteroclitus.
abstract: Fundulus heteroclitus (Actinopteri, Cyprinodontiformes, Fundulidae), with a remarkable tolerance to osmotic stress and water temperatures, are regarded as a significant evolution model. Herein, we report the assembled complete sequence of the mummichog mitochondrial genome based on the next-generation sequencing data. The mitogenome is determined to be 16 528 bp in length and shows an organization typical of vertebrate mitochondrial genomes, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region (D-loop). Overall GC content of the genome is 39.72%. Using Oryzias latipes as the outgroup, the phylogenetic analysis of 16 complete mitochondrial genomes from Cyprinodontiformes showed that F. heteroclitus together with other three Fundulus species form a cluster with strong bootstrap supports. The genus Fundulus is closely related to the genus Xenotoca.
authors.affiliation: a College of Life Science, Liaoning Normal University , Dalian , China and.|b Lamprey Research Center, Liaoning Normal University , Dalian , China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: a College of Life Science, Liaoning Normal University , Dalian , China and.|b Lamprey Research Center, Liaoning Normal University , Dalian , China.
authors.forename: Shaoshu
authors.initials: S
authors.lastname: Feng
authors.affiliation: a College of Life Science, Liaoning Normal University , Dalian , China and.|b Lamprey Research Center, Liaoning Normal University , Dalian , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: a College of Life Science, Liaoning Normal University , Dalian , China and.|b Lamprey Research Center, Liaoning Normal University , Dalian , China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004272:DNA, Mitochondrial; D005260:Female; D023781:Fundulidae / Q000235:genetics*; D050259:Genes, Mitochondrial; D059646:Genome Size; D054629:Genome, Mitochondrial; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: Illumina; Killifish; mitogenome; phylogenetics