title: Effect of p38 mitogen-activate protein kinase on MUC5AC protein expression of bile duct epithelial cells in hepatolithiasis patients.
abstract: Primary hepatolithiasis is a common bile duct disease with benign nature but complicated mechanisms. Current studies have revealed its correlation with cytokine release by chronic inflammation, which also increased mucin (MUC) synthesis. This study investigated the role of p38 mitogen-activated protein kinase (MAPK) in regulating cytokine release and mucin synthesis, in an attempt to elucidate the role of p38 signaling molecule in the pathogenesis of hepatolithiasis. In human intrahepatic bile duct endothelial cells (HIBECs), lipoprotein (LPS) was used to induce the high expression of MUC. Small interference RNA (siRNA) was then used to silencing p38 gene expression. Cytokines including interleukin (IL)-1β and tumor necrosis factor (TNF)-α were measured, along with MUC5AC protein and mRNA expression assay. The interference of p38 gene expression inhibited the release of IL-1β and TNF-α in cultured cells. It also depressed both mRNA and protein levels of MUC5A. P38 MAPK signal pathway may be involved in the formation and progression of hepatolithiasis. This study provides potential new strategy for treating hepatolithiasis using p38 MAPK signal pathway as the drug target.
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Institute of Materia Medica, South China Normal University Guangzhou, Guangdong, P.R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military University Chongqing, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Beiwang
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Canhua
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Rujin
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Shaoling
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Xianxian
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangdong, P.R. China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001649:Bile Duct Diseases / Q000378:metabolism* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D020935:MAP Kinase Signaling System; D055271:Mucin 5AC / Q000235:genetics*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Hepatolithiasis; lipoprotein; mucin; p38 MAPK