title: Inactivation of rabies virus by hydrogen peroxide.
abstract: Development of safe and protective vaccines against infectious pathogens remains a challenge. Inactivation of rabies virus is a critical step in the production of vaccines and other research reagents. Beta-propiolactone (βPL); the currently used inactivating agent for rabies virus is expensive and proved to be carcinogenic in animals. This study aimed to investigate the ability of hydrogen peroxide (H2O2) to irreversibly inactivate rabies virus without affecting its antigenicity and immunogenicity in pursuit of finding safe, effective and inexpensive alternative inactivating agents. H2O2 3% rapidly inactivated a Vero cell adapted fixed rabies virus strain designated as FRV/K within 2h of exposure without affecting its antigenicity or immunogenicity. No residual infectious virus was detected and the H2O2-inactivated vaccine proved to be safe and effective when compared with the same virus harvest inactivated with the classical inactivating agent βPL. Mice immunized with H2O2-inactivated rabies virus produced sufficient level of antibodies and were protected when challenged with lethal CVS virus. These findings reinforce the idea that H2O2 can replace βPL as inactivating agent for rabies virus to reduce time and cost of inactivation process.
authors.affiliation: Viral Control Unit, National Organization for Research and Control of Biologicals (NORCB), 51 Wezaret El-Zeraa Street, Agouza, Giza, Egypt. Electronic address: Ph.asmaa.ahmed@gmail.com.
authors.forename: Asmaa A
authors.initials: AA
authors.lastname: Abd-Elghaffar
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr Al-Aini Street, Cairo 11562, Egypt. Electronic address: amal.ali@pharma.cu.edu.eg.
authors.forename: Amal E
authors.initials: AE
authors.lastname: Ali
authors.affiliation: Viral Research and Control Lab., National Organization for Drug Control and Research (NODCAR), Kafr El-Gabal, Giza, Egypt. Electronic address: abeerboseila@gmail.com.
authors.forename: Abeer A
authors.initials: AA
authors.lastname: Boseila
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr Al-Aini Street, Cairo 11562, Egypt. Electronic address: magdy.amin@pharma.cu.edu.eg.
authors.forename: Magdy A
authors.initials: MA
authors.lastname: Amin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002522:Chlorocebus aethiops; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000737:chemistry*; D051379:Mice; D011420:Propiolactone / Q000737:chemistry; D011818:Rabies / Q000517:prevention & control; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology; D014709:Vero Cells; D038301:Virus Inactivation
chemical_list: D000914:Antibodies, Viral; D011819:Rabies Vaccines; D015164:Vaccines, Inactivated; D011420:Propiolactone; D006861:Hydrogen Peroxide
keywords: Hydrogen peroxide; Immunity; Inactivation; Rabies; Vaccine