title: [Effect of FCGR3A Polymorphisms on Antibody-dependent Cetuximab-mediated Cytotoxicity in A549 Cells].
abstract: OBJECTIVE
  To investigate the effect of FCGR3A polymorphisms on the antibody-dependent cell-mediated cytotoxicity (ADCC) activity induced by cetuximab against A549 cells.
  
  
  METHODS
  A549 cell line was used as target cells and NKTm cells as effector cells. FCGR3A polymorphisms were detected by direct sequencing. The ADCC activity mediated by cetuximab was assessed by CCK-8 assay.
  
  
  RESULTS
  Three genotypes of FCGR3A were detected:V/V,V/F,and F/F. The ADCC activity of NKTm cells with these three different genotypes mediated by cetuximab were significantly different (P=0.0015). NKTm cells with FCGR3A-158V/V genotypes had significantly higher ADCC activity than FCGR3A-V/F or F/F genotypes (P<0.01),whereas the ADCC activity between V/F and F/F genotype showed no statistical significance(P>0.05).
  
  
  CONCLUSIONS
  FCGR3A polymorphisms have an impact on ADCC activity mediated by cetuximab in NKTm cells.
authors.affiliation: Department of Medical Oncology,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Jin-yu
authors.initials: JY
authors.lastname: Li
authors.affiliation: Department of Medical Oncology,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Yan-yun
authors.initials: YY
authors.lastname: Zhu
authors.affiliation: Department of Medical Oncology,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Sheng-jie
authors.initials: SJ
authors.lastname: Sun
authors.affiliation: Department of Medical Oncology,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Shun-chang
authors.initials: SC
authors.lastname: Jiao
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D045744:Cell Line, Tumor; D000068818:Cetuximab; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D017452:Receptors, IgG
chemical_list: C496124:FCGR3A protein, human; D017452:Receptors, IgG; D000068818:Cetuximab