title: Protective role of adiponectin in a rat model of intestinal ischemia reperfusion injury.
abstract: OBJECTIVE
  To determine the potential protective role of adiponectin in intestinal ischemia reperfusion (I/R) injury.
  
  
  METHODS
  A rat model of intestinal I/R injury was established. The serum level of adiponectin in rats with intestinal I/R injury was determined by enzyme-linked immunosorbent assay (ELISA). The serum levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were also measured by ELISA. Apoptosis of intestinal cells was detected using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The production of malondialdehyde (MDA) and superoxide dismutase (SOD) and villous injury scores were also measured.
  
  
  RESULTS
  Adiponectin was downregulated in the serum of rats with intestinal I/R injury compared with sham rats. No significant changes in the expression of adiponectin receptor 1 and adiponectin receptor 2 were found between sham and I/R rats. Pre-treatment with recombinant adiponectin attenuated intestinal I/R injury. The production of pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α, in rats with intestinal I/R injury was reduced by adiponectin pre-treatment. The production of MDA was inhibited, and the release of SOD was restored by adiponectin pre-treatment in rats with intestinal I/R injury. Adiponectin pre-treatment also inhibited cell apoptosis in these rats. Treatment with the AMP-activated protein kinase (AMPK) signaling pathway inhibitor, compound C, or the heme oxygenase 1 (HO-1) inhibitor, Snpp, attenuated the protective effects of adiponectin against intestinal I/R injury.
  
  
  CONCLUSIONS
  Adiponectin exhibits protective effects against intestinal I/R injury, which may involve the AMPK/HO-1 pathway.
authors.affiliation: Xu-Hui Liu, Hai-Tao Dai, Rui-Han Chen, Zhi-Qiang Ye, Department of Emergency Care, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Liu
authors.affiliation: Xu-Hui Liu, Hai-Tao Dai, Rui-Han Chen, Zhi-Qiang Ye, Department of Emergency Care, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Yue-Wu
authors.initials: YW
authors.lastname: Yang
authors.affiliation: Xu-Hui Liu, Hai-Tao Dai, Rui-Han Chen, Zhi-Qiang Ye, Department of Emergency Care, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Dai
authors.affiliation: Xu-Hui Liu, Hai-Tao Dai, Rui-Han Chen, Zhi-Qiang Ye, Department of Emergency Care, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Song-Wang
authors.initials: SW
authors.lastname: Cai
authors.affiliation: Xu-Hui Liu, Hai-Tao Dai, Rui-Han Chen, Zhi-Qiang Ye, Department of Emergency Care, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Rui-Han
authors.initials: RH
authors.lastname: Chen
authors.affiliation: Xu-Hui Liu, Hai-Tao Dai, Rui-Han Chen, Zhi-Qiang Ye, Department of Emergency Care, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Ye
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D052242:Adiponectin / Q000008:administration & dosage* / Q000097:blood; D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005765:Gastrointestinal Agents / Q000008:administration & dosage*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D018836:Inflammation Mediators / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000098:blood supply / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D065666:Mesenteric Ischemia / Q000097:blood / Q000150:complications* / Q000473:pathology; D008641:Mesenteric Vascular Occlusion / Q000097:blood / Q000150:complications* / Q000473:pathology; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D052242:Adiponectin; C499920:Adipoq protein, rat; D015415:Biomarkers; D005765:Gastrointestinal Agents; C000594089:IL1B protein, rat; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D013482:Superoxide Dismutase; D055372:AMP-Activated Protein Kinases
keywords: Adiponectin; Intestine; Ischemia reperfusion injury