title: Alzheimer-associated presenilin 2 gene is dysregulated in rat medial temporal lobe cortex after complete brain ischemia due to cardiac arrest.
abstract: BACKGROUND
  Brain ischemia may be causally related with Alzheimer's disease. Probably, presenilin gene dysregulation may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both presenilin 1 and 2 genes in the medial temporal lobe cortex following 10-min global brain ischemia in rats.
  
  
  METHODS
  Global brain ischemia was induced by cardiac arrest in female rats that were allowed to survive for 2, 7 and 30 days. The expression of presenilin genes was evaluated in the rat medial temporal lobe cortex with the use of quantitative RT-PCR analysis.
  
  
  RESULTS
  Presenilin 1 gene expression tended to be downregulated from days 2 to 7 postischemia but at day 30, there was a reverse tendency. The greatest overexpression of presenilin 2 gene was noted at 2-nd day whilst on day 7, the expression of this gene was only modestly elevated. Eventually, at day 30 expression of presenilin 2 gene was modestly downregulated. Alterations of presenilin 2 gene expression between 2 and 7 days and between 2 and 30 days were statistically significant.
  
  
  CONCLUSIONS
  Thus, presented changes suggest that the significant dysregulation of presenilin 2 gene may be connected with a response of neuronal cells to transient global brain ischemia due to cardiac arrest. Finally, the ischemia-induced gene dysregulation may play a key role in the late onset of Alzheimer's-type dementia.
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warszawa, Poland. Electronic address: pluta@imdik.pan.pl.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Pluta
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocki
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warszawa, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Ułamek-Kozioł
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bogucka-Kocka
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Gil-Kulik
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warszawa, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Januszewski
authors.affiliation: Department of Rehabilitation and Orthopaedics, Medical University of Lublin, Lublin, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Jabłoński
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Petniak
authors.affiliation: Department of Clinical Psychology, Medical University of Lublin, Lublin, Poland.
authors.forename: Judyta
authors.initials: J
authors.lastname: Brzozowska
authors.affiliation: Warsaw Higher Humanistic School, Warszawa, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bogucki
authors.affiliation: Department of Neonate and Infant Pathology, Medical University of Lublin, Lublin, Poland.
authors.forename: Wanda
authors.initials: W
authors.lastname: Furmaga-Jabłońska
authors.affiliation: Department of Pathophysiology, Medical University of Lublin, Lublin, Poland. Electronic address: czuczwarsj@yahoo.com.
authors.forename: Stanisław J
authors.initials: SJ
authors.lastname: Czuczwar
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002545:Brain Ischemia / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006323:Heart Arrest / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D053766:Presenilin-2 / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013702:Temporal Lobe / Q000378:metabolism*
chemical_list: D053766:Presenilin-2
keywords: Alzheimer's disease; Brain ischemia; Presenilin 1 and 2; Temporal cortex; γ-Secretase