title: The plastidial retrograde signal methyl erythritol cyclopyrophosphate is a regulator of salicylic acid and jasmonic acid crosstalk.
abstract: The exquisite harmony between hormones and their corresponding signaling pathways is central to prioritizing plant responses to simultaneous and/or successive environmental trepidations. The crosstalk between jasmonic acid (JA) and salicylic acid (SA) is an established effective mechanism that optimizes and tailors plant adaptive responses. However, the underlying regulatory modules of this crosstalk are largely unknown. Global transcriptomic analyses of mutant plants (ceh1) with elevated levels of the stress-induced plastidial retrograde signaling metabolite 2-C-methyl-D-erythritol cyclopyrophosphate (MEcPP) revealed robustly induced JA marker genes, expected to be suppressed by the presence of constitutively high SA levels in the mutant background. Analyses of a range of genotypes with varying SA and MEcPP levels established the selective role of MEcPP-mediated signal(s) in induction of JA-responsive genes in the presence of elevated SA. Metabolic profiling revealed the presence of high levels of the JA precursor 12-oxo-phytodienoic acid (OPDA), but near wild type levels of JA in the ceh1 mutant plants. Analyses of coronatine-insensitive 1 (coi1)/ceh1 double mutant plants confirmed that the MEcPP-mediated induction is JA receptor COI1 dependent, potentially through elevated OPDA. These findings identify MEcPP as a previously unrecognized central regulatory module that induces JA-responsive genes in the presence of high SA, thereby staging a multifaceted plant response within the environmental context.
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Lemos
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.
authors.forename: Marta
authors.initials: M
authors.lastname: Bjornson
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Jin-Zheng
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Derrick
authors.initials: D
authors.lastname: Hicks
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Amancio de
authors.initials: Ad
authors.lastname: Souza
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Chang-Quan
authors.initials: CQ
authors.lastname: Wang
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Panyu
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Shisong
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA Genome Center, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Savithramma
authors.initials: S
authors.lastname: Dinesh-Kumar
authors.affiliation: Department of Plant Biology, University of California Davis, Davis, CA 95616, USA kdehesh@ucdavis.edu.
authors.forename: Katayoon
authors.initials: K
authors.lastname: Dehesh
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism*; D018087:Plastids / Q000187:drug effects / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; C073443:2-methyl-butan-1,2,3,4-tetraol-2,4-cyclopyrophosphate; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D020156:Salicylic Acid; D004896:Erythritol
keywords: Coronatine-insensitive1 (COI1); MEcPP (2-C-methyl-d-erythritol cyclopyrophosphate); hormonal interplay; jasmonic acid (JA); plastidial retrograde signaling metabolite; salicylic acid (SA); stress responses.