title: Use of molecular indicators of inflammation to assess the biocompatibility of all-ceramic restorations.
abstract: OBJECTIVE
  The purpose of this in vivo study was quantification of inflammatory reaction to ceramic restorations made from lithium disilicate and zirconia by measurement of the concentration of indicators of inflammation in the gingival crevicular fluid (GCF).
  
  
  METHODS
  Patients out of three prospective cohort-studies investigating three different all-ceramic restoration materials for crowns and fixed dental prostheses were included. Patients needed an associated, unrestored tooth to serve as control. GCF samples were taken from the sulcus of the restored teeth and the related controls (n = 59 pairs) and the concentrations of IL1-beta, IL-1ra and aMMP-8, as indicators of inflammation, were determined by use of ELISA tests. Periodontal status was also assessed clinically by measurement of pocket depth (PD), plaque index (PI) and bleeding on probing (BOP).
  
  
  RESULTS
  The concentrations of the inflammation indicators were not significantly different between restored teeth and controls or between lithium disilicate and zirconia restorations (p > 0.05). Furthermore, no significant difference between PD of restored teeth and controls or between groups could be shown.
  
  
  CONCLUSIONS
  Within the limitation of the study, treatment with all-ceramic restorations did not induce inflammatory reactions in a group of periodontal healthy patients. No differences between the gingiva reactions of lithium disilicate and zirconia restorations could be shown.
authors.affiliation: Department of Prosthodontics and Biomaterials, Centre for Implantology, Medical Faculty, RWTH Aachen University, Aachen, Germany.|Department of Prosthodontics, University of Heidelberg, Heidelberg, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Ariaans
authors.affiliation: Institute of Medical Statistics, RWTH Aachen University, Aachen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Heussen
authors.affiliation: Department of Prosthodontics and Biomaterials, Centre for Implantology, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Schiffer
authors.affiliation: Department of Prosthodontics and Biomaterials, Centre for Implantology, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Anna-Lena
authors.initials: AL
authors.lastname: Wienert
authors.affiliation: Institute of Immunology, RWTH Aachen University, Aachen, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Plümäkers
authors.affiliation: Institute of Immunology, RWTH Aachen University, Aachen, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Rink
authors.affiliation: Department of Prosthodontics and Biomaterials, Centre for Implantology, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Wolfart
mesh_terms: D000328:Adult; D002516:Ceramics; D003442:Crowns; D005260:Female; D005883:Gingival Crevicular Fluid; D006801:Humans; D007249:Inflammation; D053590:Interleukin 1 Receptor Antagonist Protein; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D011446:Prospective Studies
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein
keywords: Interleukin-1; Interleukin-1 receptor antagonist; aMMP-8; all-ceramic; biocompatibility; gingival crevicular fluid