title: Proteomics and bioinformatics analysis of mouse hypothalamic neurogenesis with or without EPHX2 gene deletion.
abstract: The aim of this study was to identify differently expressed proteins in the presence and absence of EPHX2 gene in mouse hypothalamus using proteomics profiling and bioinformatics analysis. This study was performed on 3 wild type (WT) and 3 EPHX2 gene global knockout (KO) mice (EPHX2(-/-)). Using the nano- electrospray ionization (ESI)-LC-MS/MS detector, we identified 31 over-expressed proteins in WT mouse hypothalamus compared to the KO counterparts. Gene Ontology (GO) annotation in terms of the protein-protein interaction network indicated that cellular metabolic process, protein metabolic process, signaling transduction and protein post-translation biological processes involved in EPHX2(-/-) regulatory network. In addition, signaling pathway enrichment analysis also highlighted chronic neurodegenerative diseases and some other signaling pathways, such as TGF-beta signaling pathway, T cell receptor signaling pathway, ErbB signaling pathway, Neurotrophin signaling pathway and MAPK signaling pathway, were strongly coupled with EPHX2 gene knockout. Further studies into the molecular functions of EPHX2 gene in hypothalamus will help to provide new perspective in neurogenesis.
authors.affiliation: Medical and Health Analytical Center, Peking University Health Science Center Beijing 100191, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Pathology, Peking University Health Science Center Beijing 100191, China.
authors.forename: Juntuo
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Cardiovascular Science, Peking University Health Science Center Beijing 100191, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Medical and Health Analytical Center, Peking University Health Science Center Beijing 100191, China.
authors.forename: Xiajuan
authors.initials: X
authors.lastname: Zou
authors.affiliation: Medical and Health Analytical Center, Peking University Health Science Center Beijing 100191, China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Medical and Health Analytical Center, Peking University Health Science Center Beijing 100191, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Medical and Health Analytical Center, Peking University Health Science Center Beijing 100191, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Physiology and Pathophysiology, Peking University Health Science Center Beijing 100191, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Physiology and Pathophysiology, Peking University Health Science Center Beijing 100191, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology; D004851:Epoxide Hydrolases / Q000235:genetics*; D017353:Gene Deletion; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055495:Neurogenesis / Q000502:physiology*; D025341:Principal Component Analysis; D060066:Protein Interaction Maps / Q000502:physiology*; D040901:Proteomics; D015398:Signal Transduction / Q000502:physiology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004851:Epoxide Hydrolases; C518648:Ephx2 protein, mouse
keywords: EPHX2; hypothalamic neurogenesis; protein network; proteomics profiling