title: Endothelial-like nitric oxide synthase immunolocalization by using gold nanoparticles and dyes.
abstract: Immunofluorescence is a biological technique that allows displaying the localization of the target molecule through a fluorescent microscope. We used a combination of gold nanoparticles and the fluorescein isothiocianate, FITC, as optical contrast agents for laser scanning confocal microscopy imaging to localize the endothelial-like nitric oxide synthase in skeletal muscle cells in a three-dimensional tissue phantom at the depth of 4µm. The FITC detected fluorescence intensity from gold-nanoparticles-labelled cells was brighter than the emission intensity from unlabelled cells.
authors.affiliation: Physics Department, University of Calabria, Rende, 87036, Italy.
authors.forename: Ramla
authors.initials: R
authors.lastname: Gary
authors.affiliation: Department of Biology Ecology and Earth Science (B.E.ST), University of Calabria, 87030 Arcavacata di Rende, CS, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Amelio
authors.affiliation: Department of Biology Ecology and Earth Science (B.E.ST), University of Calabria, 87030 Arcavacata di Rende, CS, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Garofalo
authors.affiliation: Physics Department, University of Calabria, Rende, 87036, Italy ; Institute of Cybernetics of the Georgian Technical University, Tbilisi, 0175, Georgia.
authors.forename: Gia
authors.initials: G
authors.lastname: Petriashvili
authors.affiliation: Physics Department, University of Calabria, Rende, 87036, Italy ; CNR-Nanotec UOS di Cosenza, c/o University of Calabria, Rende 87036, Italy.
authors.forename: Maria Penelope
authors.initials: MP
authors.lastname: De Santo
authors.affiliation: Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore 117543, Republic of Singapore.
authors.forename: Yuen Kwong
authors.initials: YK
authors.lastname: Ip
authors.affiliation: Physics Department, University of Calabria, Rende, 87036, Italy ; CNR-Nanotec UOS di Cosenza, c/o University of Calabria, Rende 87036, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Barberi
keywords: (170.1790) Confocal microscopy; (280.1415) Biological sensing and sensors