title: The role of structural proteomics in vaccine development: recent advances and future prospects.
abstract: Vaccines are the most effective way to fight infectious diseases saving countless lives since their introduction. Their evolution during the last century made use of the best technologies available to continuously increase their efficacy and safety. Mass spectrometry (MS) and proteomics are already playing a central role in the identification and characterization of novel antigens. Over the last years, we have been witnessing the emergence of structural proteomics in vaccinology, as a major tool for vaccine candidate discovery, antigen design and life cycle management of existing products. In this review, we describe the MS techniques associated to structural proteomics and we illustrate the contribution of structural proteomics to vaccinology discussing potential applications.
authors.affiliation: a GSK Vaccines , Siena , Italy.
authors.forename: Danilo
authors.initials: D
authors.lastname: Donnarumma
authors.affiliation: a GSK Vaccines , Siena , Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Faleri
authors.affiliation: a GSK Vaccines , Siena , Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Costantino
authors.affiliation: a GSK Vaccines , Siena , Italy.
authors.forename: Rino
authors.initials: R
authors.lastname: Rappuoli
authors.affiliation: a GSK Vaccines , Siena , Italy.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Norais
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D041961:Deuterium Exchange Measurement; D018604:Epitope Mapping; D006801:Humans; D013058:Mass Spectrometry; D011487:Protein Conformation; D040901:Proteomics / Q000379:methods*; D014612:Vaccines / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000941:Antigens; D014612:Vaccines
keywords: Structural proteomics; covalent labeling; cross-linking; hydrogen deuterium exchange; ion mobility; limited proteolysis; mass spectrometry; vaccines