title: Identification and characterization of chalcone synthase cDNAs (NnCHS) from Nelumbo nucifera.
abstract: Chalcone synthase (CHS) catalyzes the first committed step in flavonoids biosynthetic pathway. In this study, six full-length cDNAs (NnCHS) encoding CHS from Nelumbo nucifera were successfully isolated, using rapid amplification cDNA end (RACE) assay. The obtained cDNAs were 1426 bp in size, containing a 1167 bp open reading frame coding 389 amino acids. Exons-intron architecture of NnCHS gene was illustrated, consisting two exons inserted by a 426 bp intron. The putative NnCHS possessed all the conserved active sites for CHS function as well as the family signature. Phylogenetic analysis revealed that NnCHS shared high homology with CHS from high plants, and the homology-based structural modeling showed that NnCHS had the typical structure of CHS. Moreover, Real-time PCR assays demonstrated that NnCHS mRNAs were expressed in various tissues of N. nucifera, with the highest expression in red flower and lowest level in the leaves. Moreover, patterns of NnCHS expression illustrated short-time wounding or low temperature significantly induced the up-regulation of NnCHS mRNA.
authors.affiliation: Henan University of Technology College of Biological Engineering Zhengzhou China.
authors.forename: C
authors.initials: C
authors.lastname: Dong
authors.affiliation: Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China.
authors.forename: A Q
authors.initials: AQ
authors.lastname: Yu
authors.affiliation: Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China.
authors.forename: M L
authors.initials: ML
authors.lastname: Wang
authors.affiliation: Guangchang White Lotus Research Institute Fuzhou China.
authors.forename: X W
authors.initials: XW
authors.lastname: Zheng
authors.affiliation: Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China.
authors.forename: Y
authors.initials: Y
authors.lastname: Diao
authors.affiliation: Guangchang White Lotus Research Institute Fuzhou China.
authors.forename: K Q
authors.initials: KQ
authors.lastname: Xie
authors.affiliation: Wuhan University Lotus Center Wuhan China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Zhou
authors.affiliation: Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China huzhongli@whu.edu.cn.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Hu
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005091:Exons; D035264:Flowers / Q000201:enzymology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D007438:Introns; D008958:Models, Molecular; D008969:Molecular Sequence Data; D031653:Nelumbo / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D040681:Structural Homology, Protein
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D011994:Recombinant Proteins; D000217:Acyltransferases; C020141:flavanone synthetase