title: Trichocitrin, a new fusicoccane diterpene from the marine brown alga-endophytic fungus Trichoderma citrinoviride cf-27.
abstract: One new diterpene, trichocitrin (1), and four known secondary metabolites, nafuredin (2), 5-hydroxy-2,3-dimethyl-7-methoxychromone (3), 24-methylenecycloartanol (4) and citrostadienol (5), were isolated from the culture of marine brown alga-endophytic Trichoderma citrinoviride cf-27. Trichocitrin (1) represents the first Trichoderma-derived and furan-bearing fusicoccane diterpene, and its structure and relative configuration were identified by analysis of 1D/2D NMR and mass spectroscopic data. Compounds 1 and 2 exhibited 8.0- and 9.5-mm inhibition zones, respectively, against Escherichia coli at 20 μg/disc and 54.1 and 36.7% growth inhibition, respectively, of Prorocentrum donghaiense at 80 μg/mL.
authors.affiliation: a Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai , China.|b Department of Basic Sciences , Naval Aeronautical and Astronautical University , Yantai , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Liang
authors.affiliation: a Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai , China.
authors.forename: Feng-Ping
authors.initials: FP
authors.lastname: Miao
authors.affiliation: a Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai , China.|c University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Yin-Ping
authors.initials: YP
authors.lastname: Song
authors.affiliation: a Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai , China.
authors.forename: Zhan-Yong
authors.initials: ZY
authors.lastname: Guo
authors.affiliation: a Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai , China.
authors.forename: Nai-Yun
authors.initials: NY
authors.lastname: Ji
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D021241:Spectrometry, Mass, Electrospray Ionization; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes; C000617284:trichocitrin
keywords: Trichoderma citrinoviride; diterpene; nafuredin; trichocitrin