title: Preliminary fsLIBS study on bone tumors.
abstract: The aim of this study is to evaluate the capability of femtosecond Laser Induced Breakdown Spectroscopy (fsLIBS) to discriminate between normal and cancerous bone, with implications to femtosecond laser surgery procedures. The main advantage of using femtosecond lasers for surgery is that the same laser that is being used to ablate can also be used for a feedback system to prevent ablation of certain tissues. For bone tumor removal, this technique has the potential to reduce the number of repeat surgeries that currently must be performed due to incomplete removal of the tumor mass. In this paper, we performed fsLIBS on primary bone tumor, secondary tumor in bone, and normal bone. These tissues were excised from consenting patients and processed through the UC Davis Cancer Center Biorepository. For comparison, each tumor sample had a matched normal bone sample. fsLIBS was performed to characterize the spectral signatures of each tissue type. A minimum of 20 spectra were acquired for each sample. We did not detect significant differences between the fsLIBS spectra of secondary bone tumors and their matched normal bone samples, likely due to the heterogeneous nature of secondary bone tumors, with normal and cancerous tissue intermingling. However, we did observe an increase in the fsLIBS magnesium peak intensity relative to the calcium peak intensity for the primary bone tumor samples compared to the normal bone samples. These results show the potential of using femtosecond lasers for both ablation and a real-time feedback control system for treatment of primary bone tumors.
authors.affiliation: Center for Biophotonics, University of California, Davis, 95616, USA ; Department of Biomedical Engineering, University of California, Davis, 95616, USA ; These authors contributed equally to this work.
authors.forename: Ruby K
authors.initials: RK
authors.lastname: Gill
authors.affiliation: Center for Biophotonics, University of California, Davis, 95616, USA ; Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui, China ; These authors contributed equally to this work.
authors.forename: Zachary J
authors.initials: ZJ
authors.lastname: Smith
authors.affiliation: Department of Neurological Surgery, University of California, Davis, 95616, USA.
authors.forename: Ripul R
authors.initials: RR
authors.lastname: Panchal
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Davis, 95616, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Bishop
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Davis, 95616, USA.
authors.forename: Regina
authors.initials: R
authors.lastname: Gandour-Edwards
authors.affiliation: Center for Biophotonics, University of California, Davis, 95616, USA ; Department of Pathology and Laboratory Medicine, University of California, Davis, 95616, USA ; These authors contributed equally to this work ; swachsmann@ucdavis.edu.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wachsmann-Hogiu
keywords: (170.1020) Ablation of tissue; (170.4730) Optical pathology; (170.6510) Spectroscopy, tissue diagnostics; (300.0300) Spectroscopy; (300.6365) Spectroscopy, laser induced breakdown