title: UbcD4, an ortholog of E2-25K/Ube2K, is essential for activation of the immune deficiency pathway in Drosophila.
abstract: Ubiquitination is a key regulatory mechanism in the immune deficiency (IMD) pathway in Drosophila. In this study, we first developed a simple immunoblot method to identify components involved in this pathway. Considering the emerging roles of ubiquitin-conjugating enzymes (E2s) in determining ubiquitin chain types and ubiquitination speed, we screened for E2s required for IMD activation. We found that UbcD4, in addition to the previously reported E2s Effete and Bendless, was required for activation of the IMD pathway. RNAi-mediated knockdown of the UbcD4 ortholog, E2-25K/Ube2K, inhibited TNFα- and LPS-mediated activation of the NF-κB pathway, implying that UbcD4 and E2-25K/Ube2K play a conserved role as positive regulators in both pathways.
authors.affiliation: School of Life Sciences, Gwangju Institute of Science & Technology (GIST), Gwangju 500-712, South Korea.
authors.forename: Eun Sil
authors.initials: ES
authors.lastname: Park
authors.affiliation: School of Life Sciences, Gwangju Institute of Science & Technology (GIST), Gwangju 500-712, South Korea.
authors.forename: Muthukumar
authors.initials: M
authors.lastname: Elangovan
authors.affiliation: School of Life Sciences, Gwangju Institute of Science & Technology (GIST), Gwangju 500-712, South Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: School of Life Sciences, Gwangju Institute of Science & Technology (GIST), Gwangju 500-712, South Korea. Electronic address: yjyoo@gist.ac.kr.
authors.forename: Yung Joon
authors.initials: YJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D004330:Drosophila / Q000276:immunology*; D029721:Drosophila Proteins / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D044763:Ubiquitin-Conjugating Enzymes / Q000276:immunology*; D054875:Ubiquitination / Q000276:immunology*
chemical_list: D029721:Drosophila Proteins; C100879:UBE2K protein, human; C453059:Ubc4 protein, Drosophila; D044763:Ubiquitin-Conjugating Enzymes
keywords: E2-25K/Ube2K; IMD pathway; NF-κB pathway; UbcD4; Ubiquitin-conjugating enzyme (E2)