title: Differential proteome analysis along jejunal crypt-villus axis in piglets.
abstract: The brush-border membrane (BBM) of enterocytes is responsible for the digestion and absorption of nutrients and ions in the small intestine. To identify the BBM proteins involved in epithelial cell maturation along the crypt-villus axis, enterocytes were sequentially isolated from the villus tip to the crypt of the jejunum from 21-day-old suckling piglets. After preparation of BBM vesicles, we detected 194 proteins in the jejunal epithelial cells by isobaric tags using relative and absolute quantification (iTRAQ) techniques. Of these, 56 BBM proteins were differentially expressed along the crypt-villus axis. During differentiation, the expression of proteins related to digestion and absorption of nutrients was primarily downregulated at the upper, middle villus, or crypt compared to the villus tip, while expression of proteins related to structural and enzyme regulator proteins was largely upregulated. We verified the differences in Na(+)/K(+) -transporting ATPase, galectin-3, and an intestinal-type fatty acid binding protein by western blot or immunochemical analysis. Identification of BBM-associated proteins helps enhance our understanding of digestion and absorption in piglets and other mammals, including humans.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiong
authors.forename: Huansheng
authors.initials: H
authors.lastname: Yang
authors.affiliation: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Human Engineering and Research Center of Animal and Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific.
authors.forename: Xihai
authors.initials: X
authors.lastname: Hu
authors.affiliation: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Human Engineering and Research Center of Animal and Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Human Engineering and Research Center of Animal and Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific.
authors.forename: Biao
authors.initials: B
authors.lastname: Li
authors.affiliation: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Human Engineering and Research Center of Animal and Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific.
authors.forename: Lina
authors.initials: L
authors.lastname: Long
authors.affiliation: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Human Engineering and Research Center of Animal and Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Animal Science, College Station, TX, USA 77843-2471.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.affiliation: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center for Animal and Poultry Science, Key Laboratory of Agroecology in Subtropical Region, Scient, yinyulong@isa.ac.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D007583:Jejunum / Q000378:metabolism*; D020543:Proteome; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome