title: A Low-density Antigen Array for Detection of Disease-associated Autoantibodies in Human Plasma.
abstract: OBJECTIVE
  The ability to easily detect autoantibodies will help in the early diagnosis and treatment of certain diseases. Currently, available methods for autoantibody detection are time-consuming and cumbersome. The present study aimed to evaluate the performance of an easy-to-use antigen array developed for autoantibody detection.
  
  
  METHODS
  Plasma from 9 female donors diagnosed with ovarian cancer (test group) and 9 matched donors with no history of cancer (reference group) were screened and results were compared. Autoantibody levels ≥1.5-times the background were classified as positive.
  
  
  RESULTS
  A total of 29 autoantibodies were detected, out of which the autoantibody against osteoprotegerin was found to be significantly higher in the "test" group (p<0.001) while those against macrophage migration inhibitor factor, interleukin-2 and vascular endothelial growth factor were lower (p<0.05).
  
  
  CONCLUSIONS
  The evaluated antigen array has potential as a simple method for determining the presence/absence of up to 90 disease-associated autoantibodies in a plasma specimen.
authors.affiliation: Functional Genomics & Proteomics Unit, ITSI-Biosciences, Johnstown, PA, U.S.A. Richard@itsi.us.
authors.forename: Richard I
authors.initials: RI
authors.lastname: Somiari
authors.affiliation: Department of Interdisciplinary Oncology, The University of South Florida, Tampa, FL, U.S.A.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Sutphen
authors.affiliation: Functional Genomics & Proteomics Unit, ITSI-Biosciences, Johnstown, PA, U.S.A.
authors.forename: Kutralanathan
authors.initials: K
authors.lastname: Renganathan
authors.affiliation: Functional Genomics & Proteomics Unit, ITSI-Biosciences, Johnstown, PA, U.S.A.
authors.forename: Stephen
authors.initials: S
authors.lastname: Russell
authors.affiliation: Functional Genomics & Proteomics Unit, ITSI-Biosciences, Johnstown, PA, U.S.A.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pastwa
authors.affiliation: Biobanking & Biospecimen Science Research Unit, Windber Research Institute, Windber, PA, U.S.A.
authors.forename: Stella A
authors.initials: SA
authors.lastname: Somiari
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology*; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000097:blood* / Q000276:immunology*; D011933:Reagent Kits, Diagnostic
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic
keywords: Antigene array; autoantibody detection; autoimmune disease; ovarian cancer; plasma