title: Effect of peracetic acid on biofilms formed by Staphylococcus aureus and Listeria monocytogenes isolated from dairy plants.
abstract: This research investigated the removal of adherent cells of 4 strains of Staphylococcus aureus and 1 Listeria monocytogenes strain (previously isolated from dairy plants) from polystyrene microtiter plates using peracetic acid (PAA, 0.5%) for 15, 30, 60, and 120 s, and the inactivation of biofilms formed by those strains on stainless steel coupons using the same treatment times. In the microtiter plates, PAA removed all S. aureus at 15 s compared with control (no PAA treatment). However, L. monocytogenes biofilm was not affected by any PAA treatment. On the stainless steel surface, epifluorescence microscopy using LIVE/DEAD staining (BacLight, Molecular Probes/Thermo Fisher Scientific, Eugene, OR) showed that all strains were damaged within 15 s, with almost 100% of cells inactivated after 30 s. Results of this trial indicate that, although PAA was able to inactivate both S. aureus and L. monocytogenes monospecies biofilms on stainless steel, it was only able to remove adherent cells of S. aureus from polystyrene microplates. The correct use of PAA is critical for eliminating biofilms formed by S. aureus strains found in dairy plants, although further studies are necessary to determine the optimal PAA treatment for removing biofilms of L. monocytogenes.
authors.affiliation: University of São Paulo, School of Animal Science and Food Engineering, Department of Food Engineering, Av. Duque de Caxias Norte, 225, CEP 13635-900 Pirassununga, SP, Brazil.
authors.forename: S H I
authors.initials: SHI
authors.lastname: Lee
authors.affiliation: Federal University Rural of Rio de Janeiro (UFRRJ), Food Technology Department, CEP 23890-000, Seropédica, RJ, Brazil.
authors.forename: L P
authors.initials: LP
authors.lastname: Cappato
authors.affiliation: University of São Paulo, School of Animal Science and Food Engineering, Department of Food Engineering, Av. Duque de Caxias Norte, 225, CEP 13635-900 Pirassununga, SP, Brazil.
authors.forename: C H
authors.initials: CH
authors.lastname: Corassin
authors.affiliation: Federal Institute of Rio de Janeiro (IFRJ), Food Department, CEP 20270-021, Rio de Janeiro, RJ, Brazil.
authors.forename: A G
authors.initials: AG
authors.lastname: Cruz
authors.affiliation: University of São Paulo, School of Animal Science and Food Engineering, Department of Food Engineering, Av. Duque de Caxias Norte, 225, CEP 13635-900 Pirassununga, SP, Brazil. Electronic address: carlosaf@usp.br.
authors.forename: C A F
authors.initials: CAF
authors.lastname: Oliveira
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003612:Dairying; D004202:Disinfectants / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000502:physiology*; D008856:Microscopy, Fluorescence; D010463:Peracetic Acid / Q000494:pharmacology*; D013193:Stainless Steel; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*
chemical_list: D004202:Disinfectants; D013193:Stainless Steel; D010463:Peracetic Acid
keywords: Listeria monocytogenes; Staphylococcus aureus; biofilm; peracetic acid