title: Ionic liquid-based ultrasound-assisted extraction and aqueous two-phase system for analysis of caffeoylquinic acids from Flos Lonicerae Japonicae.
abstract: In this work, an ionic liquid-based ultrasonic-assisted extraction (ILUAE) method was developed to extract caffeoylquinic acids (CQAs) from Flos Lonicerae Japonicae (FLJ). ILUAE parameters were optimized by response surface methodology, including IL concentration, ultrasonic time, and liquid-solid ratio. Optimized ILUAE approach gained the highest extraction yields of 28.53, 18.21, 3.84mg/g for 3-O-caffeoylquinic acid (C1), 3,5-di-O-caffeoylquinic acid (C2), 3,4-di-O-caffeoylquinic acid (C3), respectively. C1-C3 are the three most abundant CQAs compounds in FLJ. The method showed comparable extraction yield and shorter extraction time compared with conventional extraction techniques. Subsequently, an aqueous two-phase system (ATPS) was applied in extraction solutions. Two trace CQAs, 5-O-caffeoylquinic acid (C4) and 4,5-di-O-caffeoylquinic acid (C5), were significantly enriched with signal to noise values increasing from less than 10 to higher than 1475. The results indicated that ILUAE and ATPS are efficient and environmentally-friendly sample extraction and enrichment techniques for CQAs from herbal medicines.
authors.affiliation: Jiangxi University of Chinese Traditional Medicine, The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine (Jiangxi University of Chinese Traditional Medicine), 56 Yangming Road, Nanchang, Jiangxi 330006, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Tan
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Chang-Jiang-Sheng
authors.initials: CJ
authors.lastname: Lai
authors.affiliation: Jiangxi University of Chinese Traditional Medicine, The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine (Jiangxi University of Chinese Traditional Medicine), 56 Yangming Road, Nanchang, Jiangxi 330006, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: OuYang
authors.affiliation: Jiangxi University of Chinese Traditional Medicine, The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine (Jiangxi University of Chinese Traditional Medicine), 56 Yangming Road, Nanchang, Jiangxi 330006, PR China.
authors.forename: Ming-Zhen
authors.initials: MZ
authors.lastname: He
authors.affiliation: Jiangxi University of Chinese Traditional Medicine, The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine (Jiangxi University of Chinese Traditional Medicine), 56 Yangming Road, Nanchang, Jiangxi 330006, PR China. Electronic address: fengyulin2003@126.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Feng
mesh_terms: D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D014465:Ultrasonics / Q000379:methods
chemical_list: D002726:Chlorogenic Acid; D004365:Drugs, Chinese Herbal; D052578:Ionic Liquids; D011801:Quinic Acid; C472707:caffeoylquinic acid; C478100:3,4-di-O-caffeoylquinic acid; C473200:5-caffeoylquinic acid
keywords: Aqueous two-phase system; Caffeoylquinic acids; Flos Lonicerae Japonicae; Ionic liquid-based ultrasonic-assisted extraction; Response surface methodology