title: Anti-heat shock protein autoantibody profiling in breast cancer using customized protein microarray.
abstract: Heat shock proteins (HSPs) are over-expressed in a wide range of human cancers. It results in the stimulation of the immune system and consequently in elevated concentration of anti-HSP autoantibodies. Elevated anti-HSP autoantibodies were found in breast cancer patients, and they are associated with tumor metastasis. Therefore, screening these autoantibodies could be of diagnostic and prognostic values. Protein microarrays have already demonstrated their great potential as a diagnostic tool. However, protein diversity requires optimization of the microarray fabrication to achieve high sensitivity and specificity. In this study, seven HSPs were immobilized on six different surface chemistries. After evaluation and optimization with purified antibodies of the six surface chemistries, two surfaces were selected to detect anti-HSP autoantibodies in breast cancer sera. Multiplex detection of anti-HSP autoantibodies allowed discrimination of breast cancer patients (50) from healthy controls (26) with a sensitivity of 86% and a specificity of 100%.
authors.affiliation: Institut des Nanotechnologies de Lyon INL-UMR5270, CNRS, Ecole Centrale de Lyon, Université de Lyon, 69134, Ecully, France.
authors.forename: Liu
authors.initials: L
authors.lastname: Shi
authors.affiliation: Institut des Nanotechnologies de Lyon INL-UMR5270, CNRS, Ecole Centrale de Lyon, Université de Lyon, 69134, Ecully, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gehin
authors.affiliation: Institut des Nanotechnologies de Lyon INL-UMR5270, CNRS, Ecole Centrale de Lyon, Université de Lyon, 69134, Ecully, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.affiliation: Institut des Nanotechnologies de Lyon INL-UMR5270, CNRS, Ecole Centrale de Lyon, Université de Lyon, 69134, Ecully, France.
authors.forename: Eliane
authors.initials: E
authors.lastname: Souteyrand
authors.affiliation: Institut de Recherche en Cancérologie de Montpellier INSERM U1194, Campus Val d'Aurelle, 34298, Montpellier, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Mangé
authors.affiliation: Institut de Recherche en Cancérologie de Montpellier INSERM U1194, Campus Val d'Aurelle, 34298, Montpellier, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Solassol
authors.affiliation: Institut des Nanotechnologies de Lyon INL-UMR5270, CNRS, Ecole Centrale de Lyon, Université de Lyon, 69134, Ecully, France. emmanuelle.laurenceau@ec-lyon.fr.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Laurenceau
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009367:Neoplasm Staging; D011446:Prospective Studies; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D006360:Heat-Shock Proteins
keywords: Autoantibodies; Breast cancer; Heat shock proteins; Protein microarray