title: Photothermal Ablation of in Situ Renal Tumor by PEG-IR780-C13 Micelles and Near-Infrared Irradiation.
abstract: PEG-IR780-C13 micelles have been demonstrated to be a novel photothermal agent with tumor-targeting property. This study was designed to explore the feasibility of applying PEG-IR780-C13 micelles and near-infrared (NIR) irradiation for thermal ablation of renal tumor by using an in situ tumor model. In addition, the potential thermal injury to normal renal tissue was evaluated. PEG-IR780-C13 micelles were intended to accumulate in renal tumor after systemic delivery. In vitro results revealed that PEG-IR780-C13 micelles were uptaken by RENCA cells mainly through caveola-mediated endocytosis and mainly distributed in late endosomes and lysosomes. Upon NIR irradiation, PEG-IR780-C13 micelles generated heat effectively both in vitro and in vivo, exhibiting a promising photothermal therapeutic property. The photothermal effect of PEG-IR780-C13 micelles could effectively destroy RENCA cells in vitro and adequately inhibit growth of in situ renal tumor in vivo. Meanwhile, PEG-IR780-C13 micelles mediated photothermal therapy (PTT) resulting in only limited injury to normal renal tissue surrounding tumor sites. Our data indicated that PEG-IR780-C13 micelles mediating PTT could generate tumor-specific heat for destruction of renal tumor in a minimally invasive way, providing a novel strategy for thermal ablation of renal tumor.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Department of Urology, Affiliated Drum Tower Hospital, Medical School of Nanjing University , Nanjing 210008, China.|Institute of Urology, Nanjing University , Nanjing 210008, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Department of Urology, Affiliated Drum Tower Hospital, Medical School of Nanjing University , Nanjing 210008, China.|Institute of Urology, Nanjing University , Nanjing 210008, China.
authors.forename: Linfeng
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Institute of Drug R&D, Medical School of Nanjing University , Nanjing 210093, China.|Jiangsu R&D Platform for Controlled & Targeted Drug Delivery, Nanjing University , Nanjing 210093, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Institute of Drug R&D, Medical School of Nanjing University , Nanjing 210093, China.|Jiangsu R&D Platform for Controlled & Targeted Drug Delivery, Nanjing University , Nanjing 210093, China.
authors.forename: Ahu
authors.initials: A
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Institute of Drug R&D, Medical School of Nanjing University , Nanjing 210093, China.|Jiangsu R&D Platform for Controlled & Targeted Drug Delivery, Nanjing University , Nanjing 210093, China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Urology, Affiliated Drum Tower Hospital, Medical School of Nanjing University , Nanjing 210008, China.|Institute of Urology, Nanjing University , Nanjing 210008, China.
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Institute of Drug R&D, Medical School of Nanjing University , Nanjing 210093, China.|Jiangsu R&D Platform for Controlled & Targeted Drug Delivery, Nanjing University , Nanjing 210093, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Urology, Affiliated Drum Tower Hospital, Medical School of Nanjing University , Nanjing 210008, China.|Institute of Urology, Nanjing University , Nanjing 210008, China.
authors.forename: Hongqian
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University , Nanjing 210093, China.|Institute of Drug R&D, Medical School of Nanjing University , Nanjing 210093, China.|Jiangsu R&D Platform for Controlled & Targeted Drug Delivery, Nanjing University , Nanjing 210093, China.
authors.forename: Yiqiao
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D005434:Flow Cytometry; D006979:Hyperthermia, Induced; D007124:Immunoenzyme Techniques; D007211:Indoles / Q000494:pharmacology*; D007259:Infrared Rays; D007680:Kidney Neoplasms / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D018613:Microscopy, Confocal; D010789:Phototherapy; D011092:Polyethylene Glycols / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared; D014407:Tumor Cells, Cultured
chemical_list: C548458:2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium; D007211:Indoles; D008823:Micelles; D011092:Polyethylene Glycols
keywords: IR780; photothermal therapy; renal tumor; thermal ablation