title: Electrophoretic Deposition of Dexamethasone-Loaded Mesoporous Silica Nanoparticles onto Poly(L-Lactic Acid)/Poly(ε-Caprolactone) Composite Scaffold for Bone Tissue Engineering.
abstract: The incorporation of microcarriers as drug delivery vehicles into polymeric scaffold for bone regeneration has aroused increasing interest. In this study, the aminated mesoporous silica nanoparticles (MSNs-NH2) were prepared and used as microcarriers for dexamethasone (DEX) loading. Poly(l-lactic acid)/poly(ε-caprolactone) (PLLA/PCL) nanofibrous scaffold was fabricated via thermally induced phase separation (TIPS) and served as template, onto which the drug-loaded MSNs-NH2 nanoparticles were deposited by electrophoretic deposition (EPD). The physicochemical and release properties of the prepared scaffolds (DEX@MSNs-NH2/PLLA/PCL) were examined, and their osteogenic activities were also evaluated through in vitro and in vivo studies. The release of DEX from the scaffolds revealed an initial rapid release followed by a slower and sustained one. The in vitro results indicated that the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited good biocompatibility to rat bone marrow-derived mesenchymal stem cells (BMSCs). Also, BMSCs cultured on the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited a higher degree of osteogenic differentiation than those cultured on PLLA/PCL and MSNs-NH2/PLLA/PCL scaffolds, in terms of alkaline phosphatase (ALP) activity, mineralized matrix formation, and osteocalcin (OCN) expression. Furthermore, the in vivo results in a calvarial defect model of Sprague-Dawley (SD) rats demonstrated that the DEX@MSNs-NH2/PLLA/PCL scaffold could significantly promote calvarial defect healing compared with the PLLA/PCL scaffold. Thus, the EPD technique provides a convenient way to incorporate osteogenic agents-containing microcarriers to polymer scaffold, and thus, prepared composite scaffold could be a potential candidate for bone tissue engineering application due to its capacity for delivery of osteogenic agents.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.|College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Qiu
authors.affiliation: Department of Radiology, Shanghai East Hospital, Tongji University School of Medicine , Shanghai 200120, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Nie
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.|College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.|College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
authors.affiliation: Reserch Center for Translational Medicine, Shanghai East Hospital, Tongji University , Shanghai 200120, China.
authors.forename: Youzhen
authors.initials: Y
authors.lastname: Wei
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.|College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Chuanglong
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D003907:Dexamethasone / Q000737:chemistry / Q000494:pharmacology; D004586:Electrophoresis; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D012886:Skull / Q000378:metabolism / Q000473:pathology; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide); D012822:Silicon Dioxide; D003907:Dexamethasone
keywords: bone tissue engineering; dexamethasone; electrophoretic deposition; mesoporous silica nanoparticles (MSNs); poly(L-lactic acid)/poly(ε−caprolactone) scaffold