title: Magnetic sensing film based on Fe₃O₄@Au-GSH molecularly imprinted polymers for the electrochemical detection of estradiol.
abstract: A novel magnetic molecularly imprinted sensing film (MMISF) was fabricated for the determination of estradiol (E2) based on magnetic glassy carbon electrode (MGCE) and magnetic molecularly imprinted polymers (MMIPs). The MMIPs were synthesized by in situ polymerization of glutathione (GSH)-functionalized gold (Au)-coated Fe3O4 (Fe3O4@Au-GSH) nanocomposites and aniline. The MMISF was constructed with MMIPs via a kind of "soft modification" where MMIPs were assembled and immobilized on the surface of MGCE or removed from it by freely installing a magnet into MGCE or not. The E2-MMIPs were obtained by MMIPs recognizing E2 from sample, and the electrochemical detection was carried out after forming the "soft modification" sensing film by putting MGCE into the E2-MMIPs suspension liquid. Afterwards, the "soft modification" MMISF was peeled off from the electrode by removing the magnet from MGCE. The interface of the electrode could be quickly refreshed through simple treatment for the next detection. The structures and morphologies of Fe3O4@Au-GSH, MMIPs and MMISF were investigated by Fourier transform infrared spectrometer, ultraviolet and visible spectrophotometer, scanning electron microscope and atomic force microscope. In addition, the MMISF was successfully used for detecting E2 in milk powder with good sensitivity, selectivity, reproducibility and efficiency. The linear range of the MMISF for E2 was 0.025-10.0μmolL(-1) with the limit of detection of 2.76nmolL(-1) (S/N= 3).
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China; Jiangsu Food and Pharmaceutical Science College, Huai'an 223003, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Han
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shen
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China.
authors.forename: Wanying
authors.initials: W
authors.lastname: Zhu
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zhu
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China. Electronic address: xueminzhou001_001@hotmail.com.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China. Electronic address: huijun_jiang2006@hotmail.com.
authors.forename: Huijun
authors.initials: H
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004958:Estradiol / Q000302:isolation & purification*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics; D054802:Molecular Imprinting; D053761:Nanocomposites / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D004958:Estradiol; D006046:Gold; D005978:Glutathione; D052203:Ferrosoferric Oxide
keywords: Estradiol; Fe(3)O(4)@Au; Magnetic; Molecularly imprinted polymers; Sensing film; Soft modification