title: Component-Resolved Diagnostic Study of Dermatophagoides Pteronyssinus Major Allergen Molecules in a Southern Chinese Cohort.
abstract: OBJECTIVE
  Little is known about component-resolved diagnosis (CRD) for Dermatophagoides pteronyssinus (Der p) sensitization in the Chinese population. We aimed to evaluate sensitization to Der p components in southern China.
  
  
  METHODS
  Two-hundred immunotherapy-naïve patients with asthma and/or rhinitis positive to specific IgE (sIgE) against Der p extract, along with 20 Der p-negative nonallergic healthy controls, were tested for sIgE against Der p 1, Der p 2, and Der p 10 using ImmunoCAP 100. Seventy-five were further examined with the ImmunoCAP Immuno Solid-phase Allergen Chip (ISAC). Der p 10-positive patients were also tested for sIgE against crude extracts of cockroach, moth, and shrimp.
  
  
  RESULTS
  In total, 183 (91.5%) of the 200 patients were sensitized to Der p 1 and/or Der p 2. The proportion of positive results and the median level of s1gE against Der p 1 were higher in children than in adults. Der p 1 and Der p 2 correlated with Der p in sIgE levels. ImmunoCAP ISAC demonstrated 100% specificity and 84% sensitivity in detecting Der p 1, Der p 2, and Der p 10 compared with ImmunoCAP 100. Sensitization to Der p 10 correlated well with sIgE to shrimp, moths, cockroaches, Pen m 1, Bla g 7, and Ani s 3.
  
  
  CONCLUSIONS
  The detection of Der p 1 and Der p 2 provided a good reflection of atopy to Der p in a Chinese cohort. Sensitization to Der p 10 may result from cross-reactivity with seafood and cockroaches in coastal southern China. ImmunoCAP ISAC may be a useful tool for CRD, with comparable performance to ImmunoCAP 100.
authors.forename: G
authors.initials: G
authors.lastname: Zeng
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: P
authors.initials: P
authors.lastname: Zheng
authors.forename: N
authors.initials: N
authors.lastname: Wei
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: B
authors.initials: B
authors.lastname: Sun
authors.forename: X
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D003058:Cockroaches / Q000276:immunology; D015331:Cohort Studies; D003546:Cysteine Endopeptidases / Q000276:immunology; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D019476:Insect Proteins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009036:Moths / Q000276:immunology; D039981:Pyroglyphidae / Q000276:immunology*; D065631:Rhinitis, Allergic / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology; D055815:Young Adult
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D019476:Insect Proteins; D014335:Tropomyosin; C111884:der p 10 protein, Dermatophagoides pteronyssinus; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1