title: Selective and compartmentalized myelin expression of HspB5.
abstract: In the present study, we reveal myelin-specific expression and targeting of mRNA and biochemical pools of HspB5 in the mouse CNS. Our observations are based on in situ hybridization, electron microscopy and co-localization with 2',3'-Cyclic-Nucleotide 3'-Phosphodiesterase (CNPase), reinforcing this myelin-selective expression. HspB5 mRNA might be targeted to these structures based on its presence in discrete clusters resembling RNA granules and the presence of a putative RNA transport signal. Further, sub-cellular fractionation of myelin membranes reveals a distinct sub-compartment-specific association and detergent solubility of HspB5. This is akin to other abundant myelin proteins and is consistent with HspB5's association with cytoskeletal/membrane assemblies. Oligodendrocytes have a pivotal role in supporting axonal function via generating and segregating the ensheathing myelin. This specialization places extreme structural and metabolic demands on this glial cell type. Our observations place HspB5 in oligodendrocytes which may require selective and specific chaperone capabilities to maintain normal function and neuronal support.
authors.affiliation: Centre for Biological Sciences, Faculty of Natural and Environmental Sciences, Building 85, University of Southampton, Southampton SO17 1BJ, UK. Electronic address: s.quraishe@soton.ac.uk.
authors.forename: S
authors.initials: S
authors.lastname: Quraishe
authors.affiliation: Centre for Biological Sciences, Faculty of Natural and Environmental Sciences, Building 85, University of Southampton, Southampton SO17 1BJ, UK.
authors.forename: A
authors.initials: A
authors.lastname: Wyttenbach
authors.affiliation: Centre for Biological Sciences, Faculty of Natural and Environmental Sciences, Building 85, University of Southampton, Southampton SO17 1BJ, UK.
authors.forename: N
authors.initials: N
authors.lastname: Matinyarare
authors.affiliation: Centre for Biological Sciences, Mailpoint 840 (room LD80b), Level D Laboratories and Pathology Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK.
authors.forename: V H
authors.initials: VH
authors.lastname: Perry
authors.affiliation: Peninsula School of Medicine and Dentistry, University of Plymouth, Devon, PL4 8AA, UK.
authors.forename: R
authors.initials: R
authors.lastname: Fern
authors.affiliation: Centre for Biological Sciences, Faculty of Natural and Environmental Sciences, Building 85, University of Southampton, Southampton SO17 1BJ, UK.
authors.forename: V
authors.initials: V
authors.lastname: O'Connor
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000375:Aging; D000818:Animals; D002490:Central Nervous System / Q000033:anatomy & histology* / Q000378:metabolism / Q000648:ultrastructure; D019295:Computational Biology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: C525420:Cryab protein, mouse; D008565:Membrane Proteins; D004676:Myelin Basic Protein; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases
keywords: CNS; alphaB-crystallin; chaperone; myelin; oligodendrocyte; small heat-shock protein