title: Evaluation of the effects of platelet-rich fibrin on bone regeneration in diabetic rabbits.
abstract: OBJECTIVE
  This study aimed to investigate the effect of platelet-rich fibrin on bone regeneration in critical size defects in the calvaria of diabetic rabbits.
  
  
  METHODS
  In total, 40 male New Zealand rabbits, were divided into two groups a non-diabetic control group (Group A) and a diabetic experimental group (Group B). Two bicortical circular defects 15 mm in diameter were created in the parietal bone of each animal. Each group was further divided into four groups: subgroup E, the defect was left empty; subgroup PRF, the defects were filled only with PRF; subgroup AB, the defects were filled with autogenous bone; subgroup AB + PRF, the defects were filled with autogenous bone combined with PRF. The animals sacrificed at 4 weeks and 8 weeks. Bone formation was assessed by micro-computed tomography (micro-CT) scanning, histological and histomorphometric analysis.
  
  
  RESULTS
  The total percent of new bone was the lowest in group A-E (6.77 ± 0.21 at 4 weeks, 11.01 ± 0.37 at 8 weeks) and highest in group A-AB + PRF (21.66 ± 0.91 at 4 weeks, 37.46 ± 1.25 at 8 weeks; p < 0.05). The mean percent of new bone was greatest in group B-AB + PRF at 4 and 8 weeks (16.87 ± 0.92, 29.59 ± 1.09, respectively) and lowest in group B-E (5.83 ± 0.09 at 4 weeks, 7.36 ± 1.02 at 8 weeks).
  
  
  CONCLUSIONS
  This study, despite its limitations, showed that PRF can be used safely and that PRF induced bone healing in diabetic rabbits.
authors.affiliation: Bülent Ecevit University Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Zonguldak, Turkey. Electronic address: cenkdurmuslar@hotmail.com.
authors.forename: M Cenk
authors.initials: MC
authors.lastname: Durmuşlar
authors.affiliation: Bülent Ecevit University Faculty of Dentistry, Department of Periodontology, Zonguldak, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Ballı
authors.affiliation: Bülent Ecevit University Faculty of Dentistry, Department of Periodontology, Zonguldak, Turkey.
authors.forename: Figen
authors.initials: F
authors.lastname: Öngöz Dede
authors.affiliation: Bülent Ecevit University Faculty of Dentistry, Department of Periodontology, Zonguldak, Turkey.
authors.forename: Şeyma
authors.initials: Ş
authors.lastname: Bozkurt Doğan
authors.affiliation: Bülent Ecevit University Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Zonguldak, Turkey.
authors.forename: A Ferhat
authors.initials: AF
authors.lastname: Mısır
authors.affiliation: Gazi University Faculty of Dentistry, Department of Oral Pathology, Ankara, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Barış
authors.affiliation: Harran University Faculty of Medicine, Department of Pharmacology, Turkey.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Yılmaz
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Anatomy, Turkey.
authors.forename: H Hamdi
authors.initials: HH
authors.lastname: Çelik
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Anatomy, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Vatansever
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D003920:Diabetes Mellitus; D005337:Fibrin / Q000494:pharmacology*; D008297:Male; D000073183:Platelet-Rich Fibrin; D011817:Rabbits; D055114:X-Ray Microtomography
chemical_list: D005337:Fibrin
keywords: Bone regeneration; Critical size; Defects; Diabetic rabbits; Platelet-rich fibrin