title: The role of Listeria monocytogenes cell wall surface anchor protein LapB in virulence, adherence, and intracellular replication.
abstract: Lmof2365_2117 is a Listeria monocytogenes putative cell wall surface anchor protein with a conserved domain found in collagen binding proteins. We constructed a deletion mutation in lmof2365_2117 in serotype 4b strain F2365, evaluated its virulence, and determined its ability to adhere and invade colonic epithelial cells and macrophages. In A/J mice, colonization of liver was significantly higher for F2365 than for F2365Δ2117. The ability of F2365Δ2117 to adhere to Caco-2 cells was significantly lower than F2365. The mutant also showed impaired ability to replicate in intestinal epithelial cell and murine macrophages relative to wild type F2365. Lmof2365_2117 contributed to L. monocytogenes attachment to catfish fillets. Because of its role in adherence to Caco-2 cells, we designated Lmof2365_2117 Listeria adhesion protein B (LapB). The carboxy terminus of LapB is similar to a domain in collagen binding proteins, but our results show that L. monocytogenes does not bind collagen.
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Swetha
authors.initials: S
authors.lastname: Reddy
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Akgul
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Attila
authors.initials: A
authors.lastname: Karsi
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Hossam
authors.initials: H
authors.lastname: Abdelhamed
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Wills
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA. Electronic address: lawrence@cvm.msstate.edu.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Lawrence
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D002460:Cell Line; D002473:Cell Wall / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D008089:Listeria monocytogenes / Q000472:pathogenicity* / Q000502:physiology*; D008088:Listeriosis / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D017384:Sequence Deletion; D014774:Virulence / Q000235:genetics
chemical_list: D008565:Membrane Proteins
keywords: Adherence; Enterocyte; Listeria monocytogenes; Macrophage; Virulence