title: Evaluation of CD40 and CD80 receptors in the colonic mucosal membrane of children with inflammatory bowel disease.
abstract: BACKGROUND
  The most prevalent inflammatory bowel diseases (IBD) include ulcerative colitis (UC) and Crohn's disease (CD). Immune processes play a vital role in the etiopathogenesis of these conditions, involving both cellular and humoral response mechanisms. The aim of this study was to quantify CD40- and CD80-positive cells in the biopsy specimens of large intestinal mucosa from children with IBD.
  
  
  METHODS
  The study comprised 38 children aged between 3-17 years (mean 11.5±3.7 years) - 20 boys (52.6 %) and 18 girls (47.4%). Eighteen patients were diagnosed with UC on the basis of clinical manifestation, endoscopic and histopathological findings. Mean age of this subgroup was 11.55±4.07 years. A group of 10 children (mean age 12.30±2.83) diagnosed with CD was also included. The control group comprised 10 IBD-free children (mean age 10.28±4.07 years). The surface expressions of CD40 and CD80 were analyzed in large intestine mucosa biopsy specimens, fixed in formaldehyde, embedded in paraffin, and cut with a microtome into 4 µm slices.
  
  
  RESULTS
  The number of CD40- and CD80-positive cells in the large intestinal mucosa of children with Crohn's disease and ulcerative colitis was significantly higher than in the controls. The highest number of CD40+ and CD80+ cells was observed in the caecal mucosal membrane of Crohn's disease patients and in the rectal mucosa of individuals with ulcerative colitis.
  
  
  CONCLUSIONS
  IBD is characterized by elevated, segment-specific, expression of CD40 and CD80.
authors.affiliation: Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kamińska
authors.affiliation: Department of Developmental Age Medicine and Paediatric Nursing, Medical University of Bialystok, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Roszko-Kirpsza
authors.affiliation: Department of Developmental Age Medicine and Paediatric Nursing, Medical University of Bialystok, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Landowski
authors.affiliation: Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szlagatys-Sidorkiewicz
authors.affiliation: Department of Pathomorphology, Medical University of Bialystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Guzińska-Ustymowicz
authors.affiliation: Department of Developmental Age Medicine and Paediatric Nursing, Medical University of Bialystok, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Maciorkowska
mesh_terms: D000293:Adolescent; D018122:B7-1 Antigen / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003093:Colitis, Ulcerative / Q000276:immunology*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D007420:Intestine, Large / Q000276:immunology*; D008297:Male; D011044:Poland; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D048008:TNF Receptor-Associated Factor 3; C090656:TRAF3 protein, human