title: Chromatin remodeling complexes in the assembly of long noncoding RNA-dependent nuclear bodies.
abstract: Paraspeckles are subnuclear structures that assemble on nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding (lnc)RNA. Paraspeckle formation requires appropriate NEAT1 biogenesis and subsequent assembly with multiple prion-like domain (PLD) containing RNA-binding proteins. We found that SWI/SNF chromatin remodeling complexes function as paraspeckle components that interact with paraspeckle proteins (PSPs) and NEAT1. SWI/SNF complexes play an essential role in paraspeckle formation that does not require their ATP-dependent chromatin remodeling activity. Instead, SWI/SNF complexes facilitate organization of the PSP interaction network required for intact paraspeckle assembly. SWI/SNF complexes may collectively bind multiple PSPs to recruit them onto NEAT1. SWI/SNF complexes are also required for Sat III (Satellite III) lncRNA-dependent formation of nuclear stress bodies under heat shock conditions. Organization of the lncRNA-dependent omega speckle in Drosophila also depends on the chromatin remodeling complex. These findings raise the possibility that a common mechanism controls the formation of lncRNA-dependent nuclear body architecture.
authors.affiliation: a Institute for Genetic Medicine; Hokkaido University ; Sapporo , Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kawaguchi
authors.affiliation: a Institute for Genetic Medicine; Hokkaido University ; Sapporo , Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Hirose
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D006801:Humans; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism*; D008322:Mammals; D008958:Models, Molecular; D011323:Primates; D062085:RNA, Long Noncoding / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors
keywords: RNA-binding protein; chromatin remodeling complex; long noncoding RNA; nuclear body; nuclear stress body; omega speckle; paraspeckle; prion-like domain (PLD)