title: Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide-induced mouse model of depression.
abstract: Substantial evidence from previous studies has suggested an association between major depressive disorder (MDD) and inflammation, and previous studies have associated prefrontal cortex (PFC) dysfunction with MDD. Systemic administration of bacterial lipopolysaccharide has been used to study inflammation-associated behavioral changes in rodents. However, proteomic studies investigating PFC protein expression in an LPS-induced mouse model of depression have yet to be conducted. Using two-dimensional electrophoresis coupled with matrix-assisted laser desorption ionization-time of ﬂight-tandem mass spectrometry, PFC proteomes were comparatively assessed in LPS-induced acute inflammation reaction mice, LPS-induced depressive-like behavior mice (Dep), and control mice. A total of 26 differentially expressed proteins were identiﬁed, two of which were selected for western blot analysis, the results of which revealed a significant increase in the expression levels of creatine kinase B and dihydropyrimidinase-like 3 in Dep mice, suggesting that changes in energy metabolism and neuro-genesis occur in the PFC of Dep mice. Further investigation on these processes and on the proteins of the PFC are required in order to elucidate the pathophysiological mechanism underlying MDD.
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Ziye
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Li
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: You
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Rao
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D003402:Creatine Kinase / Q000378:metabolism; D003863:Depression / Q000378:metabolism* / Q000503:physiopathology*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000503:physiopathology*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C526904:Dpysl3 protein, mouse; D008070:Lipopolysaccharides; D009419:Nerve Tissue Proteins; D003402:Creatine Kinase