title: Higher cardiogenic potential of iPSCs derived from cardiac versus skin stromal cells.
abstract: Prior studies have demonstrated that founder cell type could influence induced pluripotent stem cells (iPSCs) molecular and developmental properties at early passages after establishing their pluripotent state. Herein, we evaluated the persistence of a functional memory related to the tissue of origin in iPSCs from syngeneic cardiac (CStC) vs skin stromal cells (SStCs). We found that, at passages greater than 15, iPSCs from cardiac stromal cells (C-iPSCs) produced a higher number of beating embryoid bodies than iPSCs from skin stromal cells (S-iPSCs). Flow cytometry analysis revealed that dissected beating areas from C-iPSCs exhibited more Troponin-T positive cells compared to S-iPSCs. Beating areas derived from C-iPSCs displayed higher expression of cardiac markers, more hyperpolarized diastolic potentials, larger action potential amplitude and higher contractility than beaters from skin. Also, different microRNA subsets were differentially modulated in CStCs vs SStCs during the reprogramming process, potentially accounting for the higher cardiogenic potentials of C-iPSCs vs S-iPSCs. Therefore, the present work supports the existence of a founder organ memory in iPSCs obtained from the stromal component of the origin tissue.
authors.affiliation: Laboratory of Vascular Biology and Regenerative Medicine Centro Cardiologico Monzino IRCCS, Milano, 20138 Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Meraviglia
authors.affiliation: Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, California 92037, USA.
authors.forename: Jianyan
authors.initials: J
authors.lastname: Wen
authors.affiliation: aboratory of Immunology and Functional Genomics, Centro Cardiologico Monzino IRCCS, Milano, 20138 Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Piacentini
authors.affiliation: The PaceLab, Department of Biosciences, University of Milano, Milano, 20133 Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Campostrini
authors.affiliation: Del E. Webb Center for Neuroscience, Aging and Stem Cell Research, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, California 92037, USA.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center for Biomedicine, European Academy Bozen/Bolzano (EURAC) (affiliated institute of the University of Lübeck), Bolzano, 39100 Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Florio
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, University of Milano, Milano, 20133 Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Azzimato
authors.affiliation: Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, 27100 Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fassina
authors.affiliation: Laboratorio Specialistico di Ematologia, Ospedale Centrale di Bolzano, Azienda Sanitaria dell'Alto Adige, Bolzano, 39100 Italy.
authors.forename: Martin
authors.initials: M
authors.lastname: Langes
authors.affiliation: Del E. Webb Center for Neuroscience, Aging and Stem Cell Research, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, California 92037, USA.
authors.forename: Johnson
authors.initials: J
authors.lastname: Wong
authors.affiliation: CERT, Center of Excellence for Toxicological Research, INAIL, ex ISPESL, University of Parma, Parma, 43125 Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Miragoli
authors.affiliation: Division of Cardiovascular Epigenetics, Department of Cardiology, Goethe University, Frankfurt am Main, 60590 Germany.
authors.forename: Carlo
authors.initials: C
authors.lastname: Gaetano
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milano, Milano, 20122 Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Pompilio
authors.affiliation: Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata (CIMMBA), University of Milano, Milano, 20133 Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Barbuti
authors.affiliation: The PaceLab, Department of Biosciences, University of Milano, Milano, 20133 Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: DiFrancesco
authors.affiliation: Center for Biomedicine, European Academy Bozen/Bolzano (EURAC) (affiliated institute of the University of Lübeck), Bolzano, 39100 Italy.
authors.forename: Deborah
authors.initials: D
authors.lastname: Mascalzoni
authors.affiliation: Center for Biomedicine, European Academy Bozen/Bolzano (EURAC) (affiliated institute of the University of Lübeck), Bolzano, 39100 Italy.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Pramstaller
authors.affiliation: Laboratory of Immunology and Functional Genomics, Centro Cardiologico Monzino IRCCS, Milano, 20138 Italy.
authors.forename: Gualtiero I
authors.initials: GI
authors.lastname: Colombo
authors.affiliation: Departments of Medicine/Cardiology, University of California-San Diego (UCSD), La Jolla, California 92037, USA.
authors.forename: Huei-Sheng Vincent
authors.initials: HS
authors.lastname: Chen
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milano, Milano, 20122 Italy, alessandra.rossini@eurac.edu.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rossini
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism
chemical_list: D035683:MicroRNAs