title: Preparation and Characterizations of Dispersible Fluorinated Hydroxyapatite Nanoparticles with Weak Antibacterial Activity.
abstract: To develop a nanoscaled coating material for medical devices possessing weak antibacterial activity, dispersible and crystalline fluorinated hydroxyapatite (F-HAp) nanoparticles were prepared using antisintering agent to avoid calcination-induced sintering. The product was identical to fluorapatite, as determined by X-ray diffraction and Fourier transform infrared spectroscopy. The primary particles generally showed rod-shaped morphology with a length of 367 ± 67 nm and a width of 223 ± 21 nm measured by scanning electron microscopy (SEM). The dispersed average particle size (313 ± 51 nm) in ethanol analyzed by dynamic light scattering was almost the same as that obtained from the SEM images. In the evaluation of solubility in acidic aqueous solution, F-HAp and original hydroxyapatite (HAp) nanoparticles started to dissolve at around pH 3.4 and 4.2, respectively. Thus, the stability of F-HAp in a living body increased compared with original HAp. The antibacterial activity of F-HAp nanoparticles was higher than that of fluoride in sodium fluoride alone or the original HAp nanoparticles. However, it was estimated that the effect of F-HAp was much lower compared with that of silver, one of the popular antibacterial materials. Thus, the dispersed F-HAp nanoparticles possessing weak antimicrobial activity can be useful without severe damage to the living tissue.
authors.affiliation: From the *Department of Biomaterial Engineering, School of Biology-Oriented Science and Technology, Kinki University, Kinokawa, Wakayama, Japan; †Department of Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; ‡Department of Science and Technology on Food Safety, School of Biology-Oriented Science and Technology, Kinki University, Kinokawa, Wakayama, Japan; and §Technical Development Center, SofSera Corporation, Izumi, Osaka, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Furuzono
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Azuma
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Niigawa
authors.forename: Yasumichi
authors.initials: Y
authors.lastname: Kogai
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis*; D017886:Durapatite / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D017886:Durapatite