title: [Molecular typing of 12 Brucella strains isolated in Guizhou province in 2010-2013].
abstract: OBJECTIVE
  To identify and characterize the Brucella strains from Guizhou province in 2010-2013.
  
  
  METHODS
  A total of 12 strains of Brucella suspicious bacteria were isolated in Guizhou province from 2010 to 2013. Four strains (GZLL3, GZLL4, GZLL11 and SH2) were isolated from goat blood samples and eight strains (SH4, GZZY, GZSQ, GZZA, BR13001, BR13004, BR13005 and BR13006) were isolated from blood samples of patient 12 Brucella suspicious strains were identified and characterized using conventional methods. Brucella genus specific gene BCSP31-based PCR (BCSP31-PCR) was used to identify the genus of Brucella and IS711 insert sequence-based PCR (AMOS-PCR) was applied to identify the species of Brucella strains. Goats and patients originated Brucella strains were comparatively analysed using Pulse-field Gel Electrophoresis (PFGE).
  
  
  RESULTS
  Both of conventional methods and PCR identified the 12 Brucella suspicious strains as B. melitensis biotype 3. BCSP31-PCR identification results showed that a specific DNA bands (223 bp) were detected in all the 12 strains and positive control samples with no DNA band in negative samples. AMOS-PCR amplified a 731 bp-DNA bands in all the 12 strains, with 731 bp, 498 bp and 275 bp in M5, S2 and A19 strains, respectively, and no DNA band was detected in the negative control samples. PFGE analysis showed that 12 Brucella isolates from patients and goats showed consistent PFGE patterns with the digestion of restriction enzyme Xba I.
  
  
  CONCLUSIONS
  The epidemic species/type of Brucella in both human and animal in Guizhou province was B. melitensis biotype 3 and goat was the main animal source of infection of brucellosis in Guizhou province.
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Jingzhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China; Email: zjumedjun@163.com.
authors.forename: Shijun
authors.initials: S
authors.lastname: Li
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hang
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Tang
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Dingming
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institution for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Guichun
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000145:classification*; D002006:Brucellosis / Q000453:epidemiology*; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial; D006041:Goats; D006801:Humans; D058889:Molecular Typing; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial