title: The attenuation of epithelial to mesenchymal transition and induction of anoikis by gigantol in human lung cancer H460 cells.
abstract: Lung cancer has been the major cause of death within patients due to the high metastatic rate. One of the most essential processes of metastasis is the ability of cancer cells to resist the programmed cell death in a detached condition called anoikis. The discoveries of new natural compound that is able to sensitize anoikis in cancer cells have garnered the most interest in cancer pharmaceutical science. Gigantol, a bibenzyl compound extracted from Dendrobium draconis, has been a promising natural derived compound for cancer therapy due to several cytotoxic effects in cancer cells. This study has demonstrated for the first time that gigantol significantly decreases lung cancer cells' viability in a detached condition through anoikis and anchorage-independent assays. Western blotting analysis reveals that gigantol greatly decreases epithelial to mesenchymal transition (EMT) markers including N-cadherin, vimentin, and Slug leading to a significant suppression of protein kinase B (AKT), extracellular signal-regulated kinase (ERK), and caveolin-1 (cav-1) survival pathways during the detached condition. Therefore, gigantol could be a potential cancer therapeutic compound suggesting for further development for cancer therapy.
authors.affiliation: Pharmaceutical Technology (International) Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, 10330.|Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok, Thailand, 10330.
authors.forename: Thitita
authors.initials: T
authors.lastname: Unahabhokha
authors.affiliation: Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, 10330.|Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok, Thailand, 10330.
authors.forename: Pithi
authors.initials: P
authors.lastname: Chanvorachote
authors.affiliation: Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, 10330. varisa.p@pharm.chula.ac.th.|Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok, Thailand, 10330. varisa.p@pharm.chula.ac.th.
authors.forename: Varisa
authors.initials: V
authors.lastname: Pongrakhananon
mesh_terms: D023102:Anoikis / Q000187:drug effects*; D017209:Apoptosis / Q000187:drug effects*; D001632:Bibenzyls / Q000494:pharmacology*; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D051242:Caveolin 1 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000378:metabolism
chemical_list: D001632:Bibenzyls; D015820:Cadherins; D051242:Caveolin 1; D014746:Vimentin; C478099:gigantol; D006139:Guaiacol; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Anoikis; Cancer metastasis; Epithelial to mesenchymal transition; Gigantol; Lung cancer