title: [Treatment of transplantation of NT-3 gene modified olfactory ensheathing cells on experimental autoimmune encephalomyelitis in rats].
abstract: OBJECTIVE
  To explore the effects of transplantation of NT-3 gene modified olfactory ensheathing cells (OECs) on nerve function of rats with experimental autoimmune encephalomyelitis (EAE).
  
  
  METHODS
  The experimental autoimmune encephalomyelitis model was established in Lewis rats. The animals were randomly divided into 3 group: blank control group (transplantation of saline), OECs transplanted group (transplantation of OECs), and OECs-NT-3 transplanted group (transplantation of NT-3 modified OECs). The neurological function was assessed and recorded every day. The migration and distribution of transplanted cells were observed. The nerve regeneration was valued in the aspect of morphological structure by means of immunohistochemistry and retrograde tracing technique of horseradish peroxidase (HRP).
  
  
  RESULTS
  The neurological functional score of OECs-NT-3 transplanted group was obviously inferior to OECs transplanted group after transplantation (P < 0.05). A large number of OECs-NT-3 survived and migrated with axon in OECs-NT-3 transplanted group at Day 28. The number of nerve fibers in one microscopic field was much more in OECs-NT-3 transplanted group (38.8 ± 3.4, Day 28) than those in OECs transplanted group (32.5 ± 2.8, Day 28) (P < 0.05). The number of cortex neurons labeled by HRP in OECs-NT-3 transplanted group were significantly higher than those in OECs transplanted group, (P < 0.01).
  
  
  CONCLUSIONS
  NT-3 gene modified OECs have better capacities of survival and migration in EAE rats. The transplanted OECs-NT-3 can promote the regeneration of axon, reduce the injury of cortical neurons and improve the motor functions of EAE rats.
authors.affiliation: Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Shougang
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Siqin
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cong
authors.affiliation: Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China; Email: 812497109@qq.com.
authors.forename: Guangyun
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D004681:Encephalomyelitis, Autoimmune, Experimental; D020932:Nerve Growth Factor; D009416:Nerve Regeneration; D009474:Neurons; D009830:Olfactory Bulb; D051381:Rats; D011917:Rats, Inbred Lew; D012903:Smell
chemical_list: D020932:Nerve Growth Factor