title: The efficacy of conditioned media of adipose-derived stem cells combined with ablative carbon dioxide fractional resurfacing for atrophic acne scars and skin rejuvenation.
abstract: OBJECTIVE
  To evaluate the effects of conditioned medium of adipose-derived stem cells (ADSC-CM) on efficacy and side effects after fractional carbon dioxide laser resurfacing (FxCR) when treating subjects with facial atrophic acne scars or with skin rejuvenation needs.
  
  
  METHODS
  Twenty-two subjects were enrolled in the study and divided into two groups. Nine subjects were included in skin rejuvenation group and thirteen subjects were included in acne scar group, and all subjects underwent three sessions of FxCR. ADSC-CM was applied on FxCR site of one randomly selected face side. Evaluations were done at baseline, 1 week after first treatment, and 1 month after each treatment. The outcome assessments included subjective satisfaction scale; blinded clinical assessment; and the biophysical parameters of roughness, elasticity, skin hydration, transepidermal water loss (TEWL), and the erythema and melanin index. Biopsies taken from one subject in skin rejuvenation group were analyzed using hematoxylin and eosin, Masson's Trichrome, and Gomori's aldehyde fuchsin staining.
  
  
  RESULTS
  ADSC-CM combined with FxCR increased subject satisfaction, elasticity, skin hydration, and skin elasticity and decreased TEWL, roughness, and the melanin index in both acne scars and skin rejuvenation groups. Histologic analysis showed that ADSC-CM increased dermal collagen density, elastin density, and arranged them in order.
  
  
  CONCLUSIONS
  ADSC-CM with FxCR is a good combination therapy for treating atrophic acne scars and skin rejuvenation.
  
  
  BACKGROUND
  JSPH2012-082 - Registered 14 Feb 2012.
authors.affiliation: a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Bing-Rong
authors.initials: BR
authors.lastname: Zhou
authors.affiliation: a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Afzaal Ahmed
authors.initials: AA
authors.lastname: Bin Jameel
authors.affiliation: a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: b Nanjing Regenerative Medicine Engineering and Technology Research Center , Nanjing , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: b Nanjing Regenerative Medicine Engineering and Technology Research Center , Nanjing , China.
authors.forename: Shi-Lei
authors.initials: SL
authors.lastname: Guo
authors.affiliation: b Nanjing Regenerative Medicine Engineering and Technology Research Center , Nanjing , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Felicia
authors.initials: F
authors.lastname: Permatasari
authors.affiliation: a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
mesh_terms: D000152:Acne Vulgaris / Q000628:therapy*; D000273:Adipose Tissue; D000328:Adult; D002921:Cicatrix / Q000628:therapy*; D003131:Combined Modality Therapy; D005260:Female; D006801:Humans; D053685:Laser Therapy / Q000379:methods*; D054020:Lasers, Gas / Q000627:therapeutic use*; D008297:Male; D033581:Stem Cell Transplantation; D055815:Young Adult
keywords: ADSC-CM; Fractional carbon dioxide laser resurfacing; atrophic acne scars; skin rejuvenation