title: Expression of PHF20 protein contributes to good prognosis of NSCLC and is associated with Bax expression.
abstract: BACKGROUND
  Recent studies demonstrate that plant homeodomain finger protein 20 (PHF20), which was initially described as an immunogenic antigen in glioblastoma, is a putative transcriptional factor, and exhibits tumor suppressor activity. However, little is known about its expression and clinical significance in lung cancer.
  
  
  METHODS
  We investigated the expression of PHF20 in 142 cases of NSCLC tissue and 30 cases of normal lung tissue by immunohistochemical staining and downregulated PHF20 expression in SPC cell.
  
  
  RESULTS
  PHF20 expression was significantly higher in normal lung tissues than that in NSCLC tissues. The expression of PHF20 in NSCLC was significantly correlated with histological grade, p-TNM stage and lymph node metastasis. Moreover, the loss of PHF20 expression was associated with short overall survival. We also found that the expression of PHF20 was associated with Bax expression. Additionally, PHF20 markedly inhibited cell proliferation and invasion.
  
  
  CONCLUSIONS
  PHF20 may play an important role in NSCLC, and may serve as a potential therapeutic target of NSCLC.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University Shenyang 110001, China.
authors.forename: Na
authors.initials: N
authors.lastname: Tang
authors.affiliation: Department of Pathology, Liaoning Tumor Hospital Shenyang 110042, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University Shenyang 110001, China.
authors.forename: Xu-Yong
authors.initials: XY
authors.lastname: Lin
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University Shenyang 110001, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Assisted Reproduction, Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University Shenyang 110004, China.
authors.forename: Da-Lei
authors.initials: DL
authors.lastname: Yang
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University Shenyang 110001, China.
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Wang
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University Shenyang 110001, China.
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Qiu
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D034741:RNA, Small Interfering; D016019:Survival Analysis; D014157:Transcription Factors; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; C494826:BAX protein, human; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C440091:PHF20 protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors; D051028:bcl-2-Associated X Protein
keywords: Bax; Non-small-cell lung cancer; glioma-expressed antigen 2; plant homeodomain finger protein