title: Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy.
abstract: The introduction of multiphoton microscopy has dramatically broadened the scope of intravital imaging studies and has allowed researchers to validate and refine basic mechanistic concepts in many areas of biology within the context of physiologically relevant tissue microenvironments. This has also led to new insights into the behavior of immune cells at steady state, and how their behaviors are altered during an immune response. At the same time, advances in the humanized mouse model have allowed for in vivo studies of strictly human pathogens, such as HIV-1. Here, we describe in detail an intravital microscopy approach to visualize the dynamic behavior of HIV-infected T cells within the lymph nodes of live, anesthetized humanized mice.
authors.affiliation: Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy andImmunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02129, USA.
authors.forename: Radwa
authors.initials: R
authors.lastname: Sharaf
authors.affiliation: Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy andImmunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02129, USA.
authors.forename: Thorsten R
authors.initials: TR
authors.lastname: Mempel
authors.affiliation: Departments of Immunology and Medical Microbiology, University of Manitoba, 750 McDermot Ave, Rm 433, Winnipeg, MB, Canada, R3E 0T5. thomas.murooka@umanitoba.ca.
authors.forename: Thomas T
authors.initials: TT
authors.lastname: Murooka
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004867:Equipment Design; D015658:HIV Infections / Q000473:pathology* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D000069416:Intravital Microscopy / Q000295:instrumentation / Q000379:methods*; D008198:Lymph Nodes / Q000166:cytology / Q000473:pathology* / Q000821:virology*; D051379:Mice; D008866:Microsurgery / Q000379:methods; D013601:T-Lymphocytes / Q000473:pathology* / Q000821:virology*
keywords: Fluorescent protein; HIV-1; Humanized mice; In vivo imaging; Lymphocyte motility; Mean track velocity; Multiphoton intravital microscopy; Popliteal lymph node