title: Validation of N-glycan markers that improve the performance of CA19-9 in pancreatic cancer.
abstract: Pancreatic cancer (PC) has a high mortality rate because it is usually diagnosed late. Glycosylation of proteins is known to change in tumor cells during the development of PC. The objectives of this study were to identify and validate the diagnostic value of novel biomarkers based on N-glycomic profiling for PC. In total, 217 individuals including subjects with PC, pancreatitis, and healthy controls were divided randomly into a training group (n = 164) and validation groups (n = 53). Serum N-glycomic profiling was analyzed by DSA-FACE. The diagnostic model was constructed based on N-glycan markers with logistic stepwise regression. The diagnostic performance of the model was assessed further in validation cohort. The level of total core fucose residues was increased significantly in PC. Two diagnostic models designated GlycoPCtest and PCmodel (combining GlycoPCtest and CA19-9) were constructed to differentiate PC from normal. The area under the receiver operating characteristic curve (AUC) of PCmodel was higher than that of CA19-9 (0.925 vs. 0.878). The diagnostic models based on N-glycans are new, valuable, noninvasive alternatives for identifying PC. The diagnostic efficacy is improved by combined GlycoPCtest and CA19-9 for the discrimination of patients with PC from healthy controls.
authors.affiliation: Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China.
authors.forename: Ping-Ting
authors.initials: PT
authors.lastname: Zhou
authors.affiliation: Department of Surgery, Changhai Hospital, Second Military Medical University, 116 Changhai Rd, Shanghai, 200438, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Ji
authors.affiliation: Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Fang
authors.affiliation: Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China.
authors.forename: Yue-Peng
authors.initials: YP
authors.lastname: Yin
authors.affiliation: Department of Surgery, Changhai Hospital, Second Military Medical University, 116 Changhai Rd, Shanghai, 200438, China. jingang@sohu.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
authors.affiliation: Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China. gaocf1115@163.com.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D018395:CA-19-9 Antigen / Q000097:blood* / Q000235:genetics; D002240:Carbohydrate Sequence; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D005260:Female; D054794:Glycomics / Q000379:methods; D006031:Glycosylation; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood* / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D010195:Pancreatitis / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D009363:Neoplasm Proteins; D011134:Polysaccharides
keywords: Biomarker; Diagnostic model; N-glycan profiling; Pancreatic cancer