title: Disturbance of Bcl-2, Bax, Caspase-3, Ki-67 and C-myc expression in acute and subchronic exposure to benzo(a)pyrene in cervix.
abstract: Epidemiological studies have demonstrated that cigarette smoking is an important cofactor or an independent risk factor for the development of cervical cancer. Benzo(a)pyrene (BaP) is one of the most potent tobacco smoke carcinogens in tobacco smoke. BaP induced DNA damage and over expression in p53 cervical tissue of mice as demonstrated in our previous study. Here we present the findings of exposure to BaP on the expression of Bcl-2, C-myc, Ki-67, Caspase-3 and Bax genes in mouse cervix. Acute intraperitoneal administration of BaP (12.5, 25, 50, 100mg/kg body weight) to ICR female mice induced a significant increase in Bcl-2, C-myc, Ki-67 mRNA and protein level till 72h except in Bcl-2 at 24h with 12.5, 25, 50mg/kg as well as at 48h with 12.5mg/kg body weight post treatment. A significant increase was also seen in Caspase-3 and Bax mRNA and protein level with peak level at 24h and gradual decrease till 72h, however, the expression of caspase-3 increased while that of Bax decreased with increasing dose of Bap after 24h. In sub chronic intraperitoneal and oral gavage administration of BaP (2.5, 5, 10mg/kg body weight), similar significant increase was observed for all the examined genes as compared to the control and vehicle groups, however the expression of Bax decreased in a dose dependent manner. The findings of this study will help in further understanding the molecular mechanism of BaP induced carcinogenesis of cervical cancer.
authors.affiliation: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Meili
authors.initials: M
authors.lastname: Gao
authors.affiliation: School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an 710032, China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biopharmaceutics School of Pharmacy, Fourth Military Medical University, Xi'an 710032, Shaanxi, China.
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Xue
authors.affiliation: Center of Shared Experimental Facilities, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Xi'an 710032, Shaanxi, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Huqin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life and Geography, Qinghai Normal University, Xining 810008, China.
authors.forename: Huichun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
authors.forename: Walayat
authors.initials: W
authors.lastname: Shah
authors.affiliation: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Zhanwu
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: yukong@mail.xjtu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D063646:Carcinogenesis / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism*; D002584:Cervix Uteri / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D019394:Ki-67 Antigen / Q000378:metabolism*; D008813:Mice, Inbred ICR; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012907:Smoking / Q000009:adverse effects; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000378:metabolism
chemical_list: D002273:Carcinogens; D019394:Ki-67 Antigen; C489428:Myc protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C052617:Bcl2 protein, mouse; D001564:Benzo(a)pyrene; C505409:Casp3 protein, mouse; D053148:Caspase 3
keywords: Benzo(a)pyrene; Cervix; Disturbance; Genotoxic effects