title: Preparation of polydopamine-coated magnetic nanoparticles for dispersive solid-phase extraction of water-soluble synthetic colorants in beverage samples with HPLC analysis.
abstract: A facile and sensitive dispersive solid-phase extraction (D-SPE) method for the extraction and enrichment of four representative synthetic colorants prior to high performance liquid chromatography analysis was introduced. As highly efficient adsorbents, polydopamine-coated Fe3O4 nanoparticles (Fe3O4@PDA NPs) were prepared by a simple and green procedure. Several factors affecting the extraction efficiency, mainly including the polymerization time of dopamine, pH of the sample solution, the amount of adsorbent, extraction time and the desorption conditions, were systematically studied. Under the optimized conditions, the enrichment factors for the four colorants were both higher than 176. The limits of detection (LODs) for the established d-SPE-HPLC method were found to be 0.20-0.25μgL(-1), which were lower than most chromatographic methods previously reported for synthetic colorant analysis. When used for quantitative analysis, wide linearity ranges (1-500μgL(-1) for amaranth and Ponceau 4R, and 0.80-500μgL(-1) for sunset yellow and allure red) were achieved with good correlation (R(2)≥0.9995). The developed method was also successfully applied to the analysis of colorants in beverage samples with satisfactory results, demonstrating its reliability and feasibility in real sample analysis.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Chai
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Xiangyang vocational and technical college, Xiangyang 441050, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China; Analysis Center, Tianjin University, Tianjin 300072, PR China. Electronic address: dgs@tju.edu.cn.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Ding
mesh_terms: D000327:Adsorption; D001628:Beverages / Q000032:analysis; D002182:Candy / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D004396:Coloring Agents; D007211:Indoles; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine; D014867:Water
keywords: Dispersive solid-phase extraction; Fe(3)O(4)@PDA nanoparticles; HPLC; Synthetic colorants