title: MicroRNA-200a mediates nasopharyngeal carcinoma cell proliferation through the activation of nuclear factor-κB.
abstract: In nasopharyngeal carcinoma (NPC), the nuclear factor-κB (NF-κB) signaling pathway is highly active. The constitutive activation of NF-κB prompts malignant cell proliferation, and microRNAs are considered an important mediator in regulating the NF-κB signaling pathway. The current study investigated the effect of microRNA-200a (miR-200a) on NF-κB activation. Reverse transcription-quantitative polymerase chain reaction was used to quantify the relative level of miR-200a in NPC tissue samples and CNE2 cells. An MTT assay was used to investigate the effect of miR-200a on cell proliferation. To investigate the activation of NF-κB, western blotting was used to measure the protein levels of NF-κB and its downstream targets. To identify the target genes of miR-200a, a luciferase reporter assay was used. The current study demonstrated that miR-200a was upregulated in NPC tissue samples and cell lines. Overexpression of miR-200a resulted in the proliferation of CNE2 cells. Western blot analysis indicated that the protein levels of p65 increased when CNE2 cells were transfected with miR-200a mimics. Additionally, the downstream targets of miR-200a were upregulated, including vascular cell adhesion molecule, intercellular adhesion molecule and monocyte chemoattractant protein-1. The luciferase assay indicated that IκBα was the target gene of miR-200a. In conclusion, miR-200a was demonstrated to enhance NPC cell proliferation by activating the NF-κB signaling pathway.
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Zhuliang
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Delu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Subo
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Ear, Nose and Throat, People's Liberation Army 113th Hospital, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Ear, Nose and Throat, Yinzhou Hospital Affiliated to The Medical School of Ningbo University, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Jiandao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Stomatology, People's Liberation Army Navy General Hospital, Beijing 100048, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D020672:I-kappa B Proteins; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha