title: An antibody that confers plant disease resistance targets a membrane-bound glyoxal oxidase in Fusarium.
abstract: Plant germplasm resources with natural resistance against globally important toxigenic Fusarium are inadequate. CWP2, a Fusarium genus-specific antibody, confers durable resistance to different Fusarium pathogens that infect cereals and other crops, producing mycotoxins. However, the nature of the CWP2 target is not known. Thus, investigation of the gene coding for the CWP2 antibody target will likely provide critical insights into the mechanism underlying the resistance mediated by this disease-resistance antibody. Immunoblots and mass spectrometry analysis of two-dimensional electrophoresis gels containing cell wall proteins from Fusarium graminearum (Fg) revealed that a glyoxal oxidase (GLX) is the CWP2 antigen. Cellular localization studies showed that GLX is localized to the plasma membrane. This GLX efficiently catalyzes hydrogen peroxide production; this enzymatic activity was specifically inhibited by the CWP2 antibody. GLX-deletion strains of Fg, F. verticillioides (Fv) and F. oxysporum had significantly reduced virulence on plants. The GLX-deletion Fg and Fv strains had markedly reduced mycotoxin accumulation, and the expression of key genes in mycotoxin metabolism was downregulated. This study reveals a single gene-encoded and highly conserved cellular surface antigen that is specifically recognized by the disease-resistance antibody CWP2 and regulates both virulence and mycotoxin biosynthesis in Fusarium species.
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Xiu-Shi
authors.initials: XS
authors.lastname: Song
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Xing
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Li
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Zhang
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jin-He
authors.initials: JH
authors.lastname: Jiang
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zu-Quan
authors.initials: ZQ
authors.lastname: Hu
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xue
authors.affiliation: Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.|National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: Liao
mesh_terms: D000429:Alcohol Oxidoreductases / Q000276:immunology*; D000906:Antibodies / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D060467:Disease Resistance / Q000276:immunology*; D004875:Ergosterol / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D009183:Mycotoxins / Q000096:biosynthesis; D010084:Oxidation-Reduction; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology*; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D014774:Virulence
chemical_list: D000906:Antibodies; D009183:Mycotoxins; D049452:Green Fluorescent Proteins; D000429:Alcohol Oxidoreductases; C052371:glyoxal oxidase; D004875:Ergosterol
keywords: Fusarium; antibody inhibition; disease-resistance antibody; hydrogen peroxide (H2O2); membrane-bound glyoxal oxidase (GLX); mycotoxin; virulence