title: BCL11A expression in acute myeloid leukemia.
abstract: BACKGROUND
  BCL11A encodes a C2H2 type zinc-finger protein. During normal haematopoietic cell differentiation BCL11A expression is down-regulated. Data in mice suggest up-regulation of BCL11A is involved in the pathogenesis of myeloid leukaemias. BCL11A expression in persons with acute myeloid leukaemia (AML) is not systematically studied.
  
  
  OBJECTIVE
  Interrogate associations between BCL11A expression at diagnosis and clinical and laboratory valuables and outcomes in newly-diagnosed persons with AML.
  
  
  METHODS
  We determined BCL11A mRNA levels in bone marrow and blood mononuclear cells in 292 consecutive newly-diagnosed subjects with AML by reverse transcript and real-time polymerase chain reaction. Data were compared to mRNA levels in bone marrow cells of normals.
  
  
  RESULTS
  Subjects with BCL11A transcript levels at diagnosis exceeding the median value of 2.434 (±3.423 SD; 25th-75th inter-quartile range, 1.33-4.29) had higher WBC levels, a greater proportion of bone marrow myeloblasts, were more likely to be FAB M0 subtype, less likely to be FAB M3 subtype, more likely to be in the intermediate cytogenetic risk cohort, less likely to have a complex karyotype and more likely to have DNMT3A(R882) and FLT3-ITD mutations than subjects with transcript levels below the median value. In 89 subjects receiving conventional induction chemotherapy the complete remission rate was 54% (95% confidence interval [CI]; 33, 75%) in the lower BCL11A cohort and 65% (45, 85%; P=0.26) in the higher BCL11A cohort. 3 year survival was 33% (2, 65%) in the lower BCL11A cohort and 15% (0, 39%; P=0.35) in the high BCL11A cohort.
  
  
  CONCLUSIONS
  BCL11A transcript levels at diagnosis was significantly associated with several clinical and laboratory variables. There were also non-significant associations with complete remission rate and survival. These data suggest a possible role for BCL11A expression in AML biology.
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Huiquan
authors.initials: H
authors.lastname: Tao
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Guangsong
authors.initials: G
authors.lastname: Su
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Yin
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xie
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Hu
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Tan
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Zhongjuan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: Seattle Cancer Center Alliance, University of Washington Medical Center, Seattle, WA, USA.
authors.forename: Zhengwei Jenny
authors.initials: ZJ
authors.lastname: Mao
authors.affiliation: Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, PR China.
authors.forename: Hongchao
authors.initials: H
authors.lastname: Yin
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu province, China.
authors.forename: Weirong
authors.initials: W
authors.lastname: Chang
authors.affiliation: Haematology Research Centre, Division of Experimental Medicine, Department of Medicine, Imperial College London, London SW7 2AZ, UK.
authors.forename: Robert Peter
authors.initials: RP
authors.lastname: Gale
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu province, China.
authors.forename: Zixing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu province, China.
authors.forename: Depei
authors.initials: D
authors.lastname: Wu
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, Soochow University, Suzhou, Jiangsu province, China; Thrombosis and Hemostasis Key Lab of the Ministry of Health, Soochow University, Suzhou, Jiangsu province, China; Collaborative Innovation Center of Hematology, Soochow University, Suzhou, Jiangsu province, China. Electronic address: yinbin@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002648:Child; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins; D055815:Young Adult
chemical_list: C486563:BCL11A protein, human; D002352:Carrier Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins
keywords: Acute myeloid leukemia; BCL11A expression; Leukemia