title: Aldehyde dehydrogenase activity in Lactococcus chungangensis: Application in cream cheese to reduce aldehyde in alcohol metabolism.
abstract: Previous studies have shown that the metabolic capability of colonic microflora may be at least as high as that of the liver or higher than that of the whole human body. Aldehyde dehydrogenase (ALDH) is an enzyme produced by these bacteria that can metabolize acetaldehyde, produce from ethanol to acetate. Lactococcus species, which is commonly used as a starter in dairy products, was recently found to possess the ALDH gene, and the activity of this enzyme was determined. In this study, the ALDH activity of Lactococcus chungangensis CAU 28(T) and 11 other type strains in the genus Lactococcus was studied. Only 5 species, 3 of dairy origin (Lactococcus lactis ssp. lactis KCTC 3769(T), Lactococcus lactis ssp. cremoris KCCM 40699(T), and Lactococcus raffinolactis DSM 20443(T)) and 2 of nondairy origin (Lactococcus fujiensis NJ317(T) and L. chungangensis CAU 28(T)), showed ALDH activity and possessed a gene encoding ALDH. All of these strains were capable of making cream cheese. Among the strains, L. chungangensis produced cream cheese that contained the highest level of ALDH and was found to reduce the level of acetaldehyde in the serum of mice. These results predict a promising role for L. chungangensis CAU28(T) to be used in cheese that can be developed as functional food.
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
authors.forename: Maytiya
authors.initials: M
authors.lastname: Konkit
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Choi
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea. Electronic address: kimwy@cau.ac.kr.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D000079:Acetaldehyde / Q000097:blood / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002611:Cheese / Q000382:microbiology*; D000431:Ethanol / Q000378:metabolism*; D005260:Female; D006801:Humans; D013294:Lactococcus lactis / Q000201:enzymology*; D008297:Male; D051379:Mice; D013045:Species Specificity
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde
keywords: Lactococcus; acetaldehyde; alcohol metabolism; aldehyde dehydrogenase