title: [Comparison of Adipogenesis and Adipocyte Functions of 3T3-L1 Cells and Human Bone Marrow Mesenchymal Stem Cells In Vitro].
abstract: OBJECTIVE
  To compare the adipogenesis and the adipocyte function between 3T3-L1 cells and human bone marrow mesenchymal stem cells (MSCs) in vitro.
  
  
  METHODS
  By density gradient centrifugation and adherent culture, the MSCs were isolated from human bone marrow and purified. The cell morphology was observed under an inverted microscope. After the induction of adipogenic differentiation, the differentiation level was detected by oil red O staining and OD values. The expression of PPARγ, FABP4 and C/EBPα mRNA was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Adipocytes and THP-1 cells were co-cultured by adding 1 µg/ml cytarabine. The ability of chemotherapy resistance was measured after 48 h.
  
  
  RESULTS
  The Oil Red O staining and measuring the absorbance showed that the lipid content in 3T3-L1 cells group was more than that in MSCs group, and the OD value was higher than that in MSCs group (P < 0.05). The results of qRT-PCR showed that the relative expression of PPARγ, FABP4 and C/EBPα mRNA of 3T3-L1-derived adipocytes was higher than that of human bone marrow MSCs-derived adipocytes (P < 0.05). Coculture experiments showed that the number of viable THP-1 cells in the group containing adipocytes was more than that in the control group (P < 0.05). The difference between 3T3-L1 cell group and MSC group was statistically significant (P < 0.05).
  
  
  CONCLUSIONS
  The ability of adipogenesis of 3T3-L1 cells is higher than that of human bone marrow MSCs in vitro. Adipocytes can protect THP-1 cell line against cytarabine, and the effect of adipocytes from 3T3-L1 cell group is greater than that from the human bone marrow MSC group.
authors.affiliation: Medical College of Soochow University, Suzhou 215123, Jiangsu Province, China.
authors.forename: Si-Hong
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Department of Hematology, Shanghai Jiaotong University Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, Shanghai Jiaotong University Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Yuan-Mei
authors.initials: YM
authors.lastname: Zhai
authors.affiliation: Department of Hematology, Shanghai Jiaotong University Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhu
authors.affiliation: Medical College of Soochow University, Suzhou 215123, Jiangsu Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Shanghai Jiaotong University Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Hematology, Shanghai Jiaotong University Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Hematology, Shanghai Jiaotong University Sixth People's Hospital, Shanghai 200233, China. E-mail: shijun7@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes; D050156:Adipogenesis; D000818:Animals; D001854:Bone Marrow Cells; D022763:CCAAT-Enhancer-Binding Protein-alpha; D002454:Cell Differentiation; D050556:Fatty Acid-Binding Proteins; D006412:Hematopoietic Stem Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D047495:PPAR gamma; D012333:RNA, Messenger
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D012333:RNA, Messenger