title: Bacillus subtilis Spore Inner Membrane Proteome.
abstract: The endospore is the dormant form of Bacillus subtilis and many other Firmicutes. By sporulation, these spore formers can survive very harsh physical and chemical conditions. Yet, they need to go through germination to return to their growing form. The spore inner membrane (IM) has been shown to play an essential role in triggering the initiation of germination. In this study, we isolated the IM of bacterial spores, in parallel with the isolation of the membrane of vegetative cells. With the use of GeLC-MS/MS, over 900 proteins were identified from the B. subtilis spore IM preparations. By bioinformatics-based membrane protein predictions, ca. one-third could be predicted to be membrane-localized. A large number of unique proteins as well as proteins common to the two membrane proteomes were identified. In addition to previously known IM proteins, a number of IM proteins were newly identified, at least some of which are likely to provide new insights into IM physiology, unveiling proteins putatively involved in spore germination machinery and hence putative germination inhibition targets.
authors.forename: Linli
authors.initials: L
authors.lastname: Zheng
authors.forename: Wishwas
authors.initials: W
authors.lastname: Abhyankar
authors.forename: Natasja
authors.initials: N
authors.lastname: Ouwerling
authors.forename: Henk L
authors.initials: HL
authors.lastname: Dekker
authors.affiliation: Electron Microscopy Centre Amsterdam, Department of Cell Biology and Histology, Academic Medical Center , 1105 AZ Amsterdam, The Netherlands.
authors.forename: Henk
authors.initials: H
authors.lastname: van Veen
authors.affiliation: Electron Microscopy Centre Amsterdam, Department of Cell Biology and Histology, Academic Medical Center , 1105 AZ Amsterdam, The Netherlands.
authors.forename: Nicole N
authors.initials: NN
authors.lastname: van der Wel
authors.forename: Winfried
authors.initials: W
authors.lastname: Roseboom
authors.forename: Leo J
authors.initials: LJ
authors.lastname: de Koning
authors.forename: Stanley
authors.initials: S
authors.lastname: Brul
authors.forename: Chris G
authors.initials: CG
authors.lastname: de Koster
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000145:classification / Q000378:metabolism*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000145:classification / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D020543:Proteome / Q000145:classification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013171:Spores, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome
keywords: Bacillus subtilis; mass spectrometry; proteome; spore inner membrane