title: Epididymal protein ASF is a D-galactose-specific lectin with apoptotic effect on human breast cancer cell line MCF7.
abstract: Isolated caprine epididymal plasma glycoprotein "anti sticking factor" (ASF) interacts with caudal sperm surface in a D-galactose dependent manner. ASF acts as a Ca(2+) dependent soluble lectin principally activated in acidic pH. As a D-galactose specific lectin, it has a specific affinity for fibronectin as well as fibronectin receptor, i.e. integrins α5β3 and α5β1. By virtue of this particular property, it hampers the in vitro adhesion of the adherent breast cancer cell MCF7 with fibronectin. The effective anti-adhesive concentration of ASF promotes p53 dependent apoptosis in MCF7, which was established by Hoechst 33342 staining, DNA fragmentation assay, FITC tagged Annexin-V flowcytometry and western blot analysis. We suggest that ASF inhibits fibronectin-integrin interactions by binding with them and induces adhesion dependent apoptosis on adherent MCF7.
authors.affiliation: Division of Cryobiology, Centre for Rural and Cryogenic Technologies, Jadavpur University, Kolkata, 700032 West Bengal, India.
authors.forename: Debarun
authors.initials: D
authors.lastname: Roy
authors.affiliation: Cell Biology and Physiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata, 700032 West Bengal, India.
authors.forename: Kaushik
authors.initials: K
authors.lastname: Das
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032 West Bengal, India.
authors.forename: Subhasish
authors.initials: S
authors.lastname: Mondal
authors.affiliation: CU-BD Center of Excellence for Nanobiotechnology, Centre for Research in Nanoscience and Nanotechnology, Calcutta University, JD-2, Sector-III, Kolkata, 700098 West Bengal, India.
authors.forename: Debajit
authors.initials: D
authors.lastname: Bhowmick
authors.affiliation: Division of Cryobiology, Centre for Rural and Cryogenic Technologies, Jadavpur University, Kolkata, 700032 West Bengal, India.
authors.forename: Souvik
authors.initials: S
authors.lastname: Dey
authors.affiliation: Division of Cryobiology, Centre for Rural and Cryogenic Technologies, Jadavpur University, Kolkata, 700032 West Bengal, India.
authors.forename: Gopal C
authors.initials: GC
authors.lastname: Majumder
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032 West Bengal, India.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Mukherjee
authors.affiliation: Division of Cryobiology, Centre for Rural and Cryogenic Technologies, Jadavpur University, Kolkata, 700032 West Bengal, India. Electronic address: ddbhattacharyya@gmail.com.
authors.forename: Debdas
authors.initials: D
authors.lastname: Bhattacharyya
mesh_terms: D000373:Agglutinins / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002448:Cell Adhesion; D004822:Epididymis / Q000378:metabolism*; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006041:Goats; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism*; D061986:MCF-7 Cells; D008297:Male; D011485:Protein Binding; D013094:Spermatozoa / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D000373:Agglutinins; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037102:Lectins; D016159:Tumor Suppressor Protein p53
keywords: Adhesion dependent apoptosis; Epididymal anti sticking factor; Soluble lectin