title: A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.
abstract: It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.|Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Sun
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.|Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Jia
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.|Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Yang
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Meiping
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
authors.forename: Cong
authors.initials: C
authors.lastname: Mingyang
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.|Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000502:physiology*; D000040:Abscisic Acid / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D018525:Germination / Q000502:physiology; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000502:physiology*; D054046:Plant Stomata / Q000502:physiology; D036226:Seedlings / Q000254:growth & development; D016415:Sequence Alignment; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013025:Glycine max / Q000235:genetics / Q000502:physiology*
chemical_list: D048948:14-3-3 Proteins; D010937:Plant Growth Regulators; D000040:Abscisic Acid