title: Effect of camptothecin on inducible nitric oxide synthase expression in the colon cancer SW480 cell line.
abstract: As a topoisomerase I inhibitor, camptothecin (CPT) is regarded as an effective antitumor agent. In an attempt to search for its novel anticancer mechanism, the present study evaluated the effects of CPT on inducible nitric oxide synthase (iNOS) in the human colon cancer SW480 cell line when stimulated with lipopolysaccharide (LPS) and interleukin (IL)-1β. The data indicated that CPT significantly decreased NO production. Consistent with these observations, the protein and mRNA expression levels of iNOS were inhibited by CPT in a dose-dependent manner. Thus, the inhibitory effects of CPT on LPS/IL-1β-stimulated NO production were likely mediated via the inhibition of iNOS gene transcription. From these results, we propose that the inhibition of NO biosynthesis by CPT may partially underlie the efficacy of this antitumor agent.
authors.affiliation: Department of Basic Science, Zhejiang Medical College, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Xiangdi
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Basic Science, Zhejiang Medical College, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Basic Science, Zhejiang Medical College, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qiu
authors.affiliation: Department of Basic Science, Zhejiang Medical College, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xin
keywords: SW480; camptothecin; inducible nitric oxide synthase