title: Expression of Monocarboxylate Transporter Isoforms in Rat Skeletal Muscle Under Hypoxic Preconditioning and Endurance Training.
abstract: Previously, we have reported the regulation of monocarboxylate transporters (MCT)1 and MCT4 by physiological stimuli such as hypoxia and exercise. In the present study, we have evaluated the effect of hypoxic preconditioning and training on expression of different MCT isoforms in muscles. We found the increased mRNA expression of MCT1, MCT11, and MCT12 after hypoxic preconditioning with cobalt chloride and training. However, the expression of other MCT isoforms increased marginally or even reduced after hypoxic preconditioning. Only the protein expression of MCT1 increased after hypoxia preconditioning. MCT2 protein expression increased after training only and MCT4 protein expression decreased both in preconditioning and hypoxic training. Furthermore, we found decreased plasma lactate level during hypoxia preconditioning (0.74-fold), exercise (0.78-fold), and hypoxia preconditioning along with exercise (0.67-fold), which indicates increased lactate uptake by skeletal muscle. The protein-protein interactions with hypoxia inducible factor-1 and MCT isoforms were also evaluated, but no interaction was found. In conclusion, we say that almost all MCTs are expressed in red gastrocnemius muscle at the mRNA level and their expression is regulated differently under hypoxia preconditioning and exercise condition.
authors.affiliation: 1 Experimental Biology Division, Defence Institute of Physiology & Allied Sciences , Defence Research and Development Organization, Delhi, India .
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saxena
authors.affiliation: 2 Department of Biotechnology, Guru Ghasidas University , Bilaspur, India .
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Shukla
authors.affiliation: 1 Experimental Biology Division, Defence Institute of Physiology & Allied Sciences , Defence Research and Development Organization, Delhi, India .
authors.forename: Anju
authors.initials: A
authors.lastname: Bansal
mesh_terms: D000818:Animals; D000860:Hypoxia / Q000378:metabolism; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000379:methods*; D010807:Physical Endurance / Q000502:physiology*; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D027501:Monocarboxylic Acid Transporters; D020033:Protein Isoforms; D012333:RNA, Messenger
keywords: cobalt chloride; exercise; hypoxia preconditioning; monocarboxylate transporter; skeletal muscle