title: UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection.
abstract: A growing number of nuclear magnetic resonance (NMR) spectroscopic studies are impaired by the limited information content provided by the standard set of experiments conventionally recorded. This is particularly true for studies of challenging biological systems including large, unstructured, membrane-embedded and/or paramagnetic proteins. Here we introduce the concept of unified time-optimized interleaved acquisition NMR (UTOPIA-NMR) for the unified acquisition of standard high-γ (e.g. (1)H) and low-γ (e.g. (13)C) detected experiments using a single receiver. Our aim is to activate the high level of polarization and information content distributed on low-γ nuclei without disturbing conventional magnetization transfer pathways. We show that using UTOPIA-NMR we are able to recover nearly all of the normally non-used magnetization without disturbing the standard experiments. In other words, additional spectra, that can significantly increase the NMR insights, are obtained for free. While we anticipate a broad range of possible applications we demonstrate for the soluble protein Bcl-xL (ca. 21 kDa) and for OmpX in nanodiscs (ca. 160 kDa) that UTOPIA-NMR is particularly useful for challenging protein systems including perdeuterated (membrane) proteins.
authors.affiliation: Institute of Physical Biology, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Aldino
authors.initials: A
authors.lastname: Viegas
authors.affiliation: Institute of Physical Biology, Heinrich-Heine-University, Düsseldorf, Germany.|Instititue of Complex Systems, Forschungszentrum Jülich, Jülich, Germany.
authors.forename: Thibault
authors.initials: T
authors.lastname: Viennet
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.|Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan.
authors.forename: Tsyr-Yan
authors.initials: TY
authors.lastname: Yu
authors.affiliation: Bruker BioSpin GmbH, Fällanden, Switzerland.
authors.forename: Frank
authors.initials: F
authors.lastname: Schumann
authors.affiliation: Bruker BioSpin GmbH, Rheinstetten, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bermel
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wagner
authors.affiliation: Institute of Physical Biology, Heinrich-Heine-University, Düsseldorf, Germany. manuel.etzkorn@hhu.de.|Instititue of Complex Systems, Forschungszentrum Jülich, Jülich, Germany. manuel.etzkorn@hhu.de.
authors.forename: Manuel
authors.identifier: http://orcid.org/0000-0002-9796-3246
authors.initials: M
authors.lastname: Etzkorn
mesh_terms: D008565:Membrane Proteins / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
chemical_list: D008565:Membrane Proteins
keywords: 13C-detection; Interleaved acquisition; Low-γ nuclei; UTOPIA-NMR