title: [Cryptotanshinone down-regulates the expression of VEGF and inhibits angiogenesis in U2OS osteosarcoma cells].
abstract: OBJECTIVE
  To explore the effect of cryptotanshinone (CTS) on vascular endothelial growth factor (VEGF) expression in U2OS osteosarcoma cells, with a focus on angiogenesis.
  
  
  METHODS
  U2OS osteosarcoma cells cultured in vitro were divided into (20, 40, 80, 160) μmol/L CTS treated groups and control group. Real-time quantitative PCR was performed to detect the expression of VEGF mRNA in U2OS osteosarcoma cells. The VEGF protein level was determined using Western blotting and immunofluorescence cytochemistry. Cell proliferation ability was detected by CCK-8 assay. The tube formation assay in vitro was used to observe the angiogenesis ability.
  
  
  RESULTS
  CTS inhibited the levels of VEGF mRNA and protein in a dose-dependent manner in U2OS osteosarcoma cells obviously. CCK-8 assay indicated that CTS inhibited the proliferation of U2OS osteosarcoma cells. The tube formation assay in vitro revealed that CTS inhibited the angiogenesis ability.
  
  
  CONCLUSIONS
  CTS could down-regulate the expression of VEGF and inhibit angiogenesis in U2OS osteosarcoma cells.
authors.affiliation: Department of Traditional Chinese Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
authors.forename: Yameng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, 301 Hospital, Beijing 100853, China.
authors.forename: Quanbo
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Department of Orthopaedics, 301 Hospital, Beijing 100853, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, 301 Hospital, Beijing 100853, China. *Corresponding author, E-mail: yanwang301@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008856:Microscopy, Fluorescence; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control; D018919:Neovascularization, Physiologic / Q000187:drug effects; D012516:Osteosarcoma / Q000098:blood supply / Q000235:genetics / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D042461:Vascular Endothelial Growth Factor A; C037886:cryptotanshinone