title: Sedimentibacter acidaminivoranssp. nov., an anaerobic, amino-acid-utilizing bacterium isolated from marine subsurface sediment.
abstract: A novel, anaerobic bacterium, strain MO-SEDIT, was isolated from a methanogenic microbial community, which was originally obtained from marine subsurface sediments collected from off the Shimokita Peninsula of Japan. Cells were Gram-stain-negative, non-motile, non-spore-forming rods, 0.4-1.4 μm long by 0.4-0.6 μm wide. The cells also formed long filaments of up to about 11 μm. The strain grew on amino acids (i.e. valine, leucine, isoleucine, methionine, glycine, phenylalanine, tryptophan, lysine and arginine), pyruvate and melezitose in the presence of yeast extract. Growth was observed at 4-37 °C (optimally at 30 °C), at pH 6.0 and 8.5 (optimally at 7.0-7.5) and in 0-60 g l- 1NaCl (optimally 20 g NaCl l- 1). The G+C content of the DNA was 32.0 mol%. The polar lipids of strain MO-SEDITwere phosphatidylglycerol, phosphatidyl lipids and unknown lipids. The major cellular fatty acids (>10 % of the total) were C14 : 0, C16 : 1ω9 and C16 : 0dimethyl aldehyde. Comparative sequence analysis of the 16S rRNA gene showed that strain MO-SEDITwas affiliated with the genusSedimentibacterwithin the phylumFirmicutes. It was related most closely to the type strain ofSedimentibacter saalensis(94 % sequence similarity). Based on the phenotypic and genetic characteristics, strain MO-SEDITis considered to represent a novel species of the genusSedimentibacter, for which the nameSedimentibacter acidaminivoranssp. nov. is proposed. The type strain is MO-SEDIT( = JCM 17293T= DSM 24004T).
authors.affiliation: Research and Development Center for Marine Resources,JAMSTEC, Kanagawa 237-0061,Japan.|Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science & Technology (JAMSTEC),Yokosuka, Kanagawa 237-0061,Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imachi
authors.affiliation: Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science & Technology (JAMSTEC),Yokosuka, Kanagawa 237-0061,Japan.
authors.forename: Sanae
authors.initials: S
authors.lastname: Sakai
authors.affiliation: Marine Functional Biology Group,JAMSTEC, Yokosuka, Kanagawa 237-0061,Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kubota
authors.affiliation: Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science & Technology (JAMSTEC),Yokosuka, Kanagawa 237-0061,Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miyazaki
authors.affiliation: Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science & Technology (JAMSTEC),Yokosuka, Kanagawa 237-0061,Japan.|Department of Environmental Systems Engineering, Nagaoka University of Technology,Nagaoka, Niigata 940-2188,Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science & Technology (JAMSTEC),Yokosuka, Kanagawa 237-0061,Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Takai