title: MicroRNA Profiles in Spontaneous Decidualized Menstrual Endometrium and Early Pregnancy Decidua with Successfully Implanted Embryos.
abstract: To comparatively analyze the human microRNA (miRNA) profiles between spontaneous decidualized menstrual endometrium and early pregnancy decidua by an in-depth sequencing of miRNAs. The specific miRNAs expressed at conception might be involved in pregnancy establishment and expression of let-7f-5p and let-7g-5p was experimentally up-regulated or inhibited to assess the effect on the expression of IGF2BP-1 and IGF2R in vitro, respectively. Samples of endometria and deciduas were obtained from 25 women who suffered from tubal or male factor subfertility and from 35 early pregnant women who underwent pregnancy termination at 6-8 weeks gestation were irrespectively collected and comparatively analyzed by miRNA sequencing and differential expression of known and novel miRNAs was analyzed using bioinformatics. The 2042 miRNA expression was analyzed in the study and the differential expression of six miRNAs was validated by qRT-PCR. The expression of four miRNAs in decidua samples was down-regulated (miR-34c, miR-92a, miR-181a-5p, and miR-191), whereas the expression of miR-10a-5p and let-7f-5p was significantly up-regulated. The expression of IGF2BP-1 and IGF2R declined and increased with overexpression and inhibition of let-7f-5p and let-7g-5p, respectively. Changes in the expression of particular miRNAs might play a role in the physiology of decidualization following successful embryo implantation, ultimately resulting in continuous decidualization.
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.|Department of Gynecology and Obstetrics, Inner Mongolia Medical University affiliated hospital, Hohhot, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Gao
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Sun
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Gong
authors.affiliation: Department of Gynecology and Obstetrics, Inner Mongolia Medical University affiliated hospital, Hohhot, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Li
mesh_terms: D000022:Abortion, Spontaneous; D000328:Adult; D001483:Base Sequence; D003656:Decidua / Q000378:metabolism*; D015536:Down-Regulation; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017527:Receptor, IGF Type 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C406144:IGF2BP1 protein, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D016601:RNA-Binding Proteins; D017527:Receptor, IGF Type 2