title: Streptomyces sanglieri which colonised and enhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninense in in vitro studies.
abstract: Actinomycete strain AUM 00500 was 99.5 % similar to Streptomyces sanglieri NBRC 100784(T) and was evaluated for antagonistic activity towards Ganoderma boninense, the causative fungus of basal stem rot of oil palm. The strain showed strong antifungal activity towards G. boninense in in vitro and SEM analysis showed various modes of inhibition of the fungus. Ethyl acetate extracts of single culture and inhibition zone of cross-plug culture by HPLC indicated that strain AUM 00500 produced two different antibiotics of the glutarimide group namely cycloheximide and actiphenol. In greenhouse trials, oil palm seed treated with spores of S. sanglieri strain AUM 00500 at 10(9) cfu/ml showed significant (P < 0.05) increase in oil palm seedlings growth when compared to the control. Streptomyces sanglieri strain AUM 00500 successfully colonised the epidermal surface of the roots of treated oil palm seedlings and it was recovered from root fragments plated on starch casein agar.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.|Mushroom Research Centre, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: A B
authors.initials: AB
authors.lastname: Nur Azura
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: M
authors.initials: M
authors.lastname: Yusoff
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.|Mushroom Research Centre, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: G Y A
authors.initials: GY
authors.lastname: Tan
authors.affiliation: Mushroom Research Centre, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Jegadeesh
authors.affiliation: Sime Darby Technology Centre Sdn. Bhd., 1st Floor, Block B, UPM-MTDC Technology Centre III, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: D R
authors.initials: DR
authors.lastname: Appleton
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. viki@um.edu.my.|Mushroom Research Centre, University of Malaya, 50603, Kuala Lumpur, Malaysia. viki@um.edu.my.
authors.forename: S
authors.initials: S
authors.lastname: Vikineswary
mesh_terms: D000935:Antifungal Agents; D028023:Arecaceae / Q000254:growth & development* / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D032902:Ganoderma / Q000502:physiology*; D000073878:Palm Oil; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology; D010938:Plant Oils; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D036226:Seedlings / Q000254:growth & development* / Q000382:microbiology*; D013302:Streptomyces / Q000254:growth & development / Q000502:physiology*
chemical_list: D000935:Antifungal Agents; D010938:Plant Oils; D000073878:Palm Oil
keywords: Actiphenol; Antagonistic effect; Cycloheximide; Ganoderma boninense; Streptomyces sanglieri