title: Altered expression of mRNA profiles in blood of early-onset schizophrenia.
abstract: To identify gene expression abnormalities in schizophrenia (SZ), we generated whole-genome gene expression profiles using microarrays on peripheral blood mononuclear cells (PBMCs) from 18 early-onset SZ cases and 12 controls. We detected 84 transcripts differentially expressed by diagnostic status, with 82 genes being upregulated and 2 downregulated. We identified two SZ associated gene coexpression modules (green and red), including 446 genes . The green module is positively correlated with SZ, encompassing predominantly up-regulated genes in SZ; while the red module was negatively correlated with disease status, involving mostly nominally down-regulated genes in SZ. The olfactory transduction pathway was the most enriched pathways for the genes within the two modules. The expression levels of several hub genes, including AKT1, BRCA1, CCDC134, UBD, and ZIC2 were validated using real-time quantitative PCR. Our findings indicate that mRNA coexpression abnormalities may serve as a promising mechanism underlying the development of SZ.
authors.affiliation: Department of Psychiatry, First Hospital /First Clinical Medical College of Shanxi Medical University, Taiyuan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Unit on Statistical Genomics, Division of Intramural Research Program, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yao Shugart
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Zaohuo
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Jin
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Mental Health, Sixth Hospital, Peking University; Beijing 100191, China.|Key Laboratory of Mental Health, Ministry of Health &National Clinical Research Center for Mental Disorders (Peking University), Beijing, 100191, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: Institute of Mental Health, Sixth Hospital, Peking University; Beijing 100191, China.|Key Laboratory of Mental Health, Ministry of Health &National Clinical Research Center for Mental Disorders (Peking University), Beijing, 100191, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Yue
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Institute of Mental Health, Sixth Hospital, Peking University; Beijing 100191, China.|Key Laboratory of Mental Health, Ministry of Health &National Clinical Research Center for Mental Disorders (Peking University), Beijing, 100191, China.|Peking-Tsinghua Center for Life Sciences/ PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, 100871, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D017668:Age of Onset; D016022:Case-Control Studies; D002648:Child; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D015203:Reproducibility of Results; D012559:Schizophrenia / Q000097:blood / Q000235:genetics*; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger