title: VEGF/VEGFR2 Axis in Periodontal Disease Progression and Angiogenesis: Basic Approach for a New Therapeutic Strategy.
abstract: Periodontal lesions are associated with activation of pathological angiogenesis and a high number of newly-formed blood vessels. Most angiogenic growth factors have been studied in the crevicular fluid or serum, but tissue correlations with vascular density or endothelial proliferation, are very rare, even inexistent. We assessed the VEGF/VEGFR2 axis expression in a multimodal fashion, in both epithelial and stromal compartments, with emphasis to endothelial proliferation and severity of periodontal lesions. Compared to normal gingiva, negative for VEGF/VEGFR2, periodontal lesions had a progressive increase for these markers from low to severe periodontal lesions. The transition from low to moderate periodontal lesions represents the milestone in disease progression and implies an active angiogenesis based on the highest angiogenic parameter variability observed for these lesions. Epithelial vascularization was firstly observed in moderate periodontal lesions and persists during severe periodontal disease. All the parameters used to quantify angiogenesis in periodontal lesions, were significantly increased in severe periodontal lesions dependent on VEGF expression in both the epithelial and stromal compartment. Our results support the use of anti-VEGF/VEGFR2-targeted therapy as adjuvant treatment for severe periodontal lesions.
authors.affiliation: Vladau Dental Clinic, Brasov, Romania.
authors.forename: Mircea
authors.initials: M
authors.lastname: Vladau
authors.affiliation: Department of Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania ancacimpean1972@yahoo.com.
authors.forename: Anca Maria
authors.initials: AM
authors.lastname: Cimpean
authors.affiliation: Department of Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania.
authors.forename: Raluca Amalia
authors.initials: RA
authors.lastname: Balica
authors.affiliation: Department of Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania.
authors.forename: Andreea Adriana
authors.initials: AA
authors.lastname: Jitariu
authors.affiliation: Department of Dental Preventive Medicine, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania.
authors.forename: Ramona Amina
authors.initials: RA
authors.lastname: Popovici
authors.affiliation: Department of Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania.
authors.forename: Marius
authors.initials: M
authors.lastname: Raica
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology; D018450:Disease Progression; D042783:Endothelial Cells / Q000378:metabolism; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D010510:Periodontal Diseases / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: Periodontal disease; VEGF; VEGFR2; endothelial proliferation; microvessel density