title: Pseudoclavibacter endophyticussp. nov., isolated from roots ofGlycyrrhiza uralensis.
abstract: A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007T, was isolated from healthy roots ofGlycyrrhiza uralensisF. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007Thad highest similarities with members of the genusPseudoclavibacter, includingPseudoclavibacter chungangensisCAU 59T(96.98 % 16S rRNA gene sequence similarity),Pseudoclavibacter helvolusDSM 20419T(96.43 %) andPseudoclavibacter terraeTHG-MD12T(96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007Tclustered with members of the genusPseudoclavibacter, and formed a distinct clade withP. chungangensisCAU 59T. The polar lipids detected for strain EGI 60007Twere phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007Tshowed typical characteristics of the genusPseudoclavibacter, with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C15 : 0, anteiso-C17 : 0and iso-C16 : 0as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007Tis considered to represent a novel species of the genusPseudoclavibacter, for which the namePseudoclavibacter endophyticussp. nov. is proposed. The type strain is EGI 60007T( = CGMCC 1.15081T= KCTC 39112T= DSM 29943T).
authors.affiliation: Kunming Medical University Haiyuan College,Kunming, 650106,PR China.
authors.forename: Yan-Qiong
authors.initials: YQ
authors.lastname: Li
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences,Urumqi, 830011,PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091,PR China.
authors.forename: Yu-Song
authors.initials: YS
authors.lastname: Fu
authors.affiliation: Kunming Medical University Haiyuan College,Kunming, 650106,PR China.
authors.forename: Zhao-Qiong
authors.initials: ZQ
authors.lastname: Cui
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275,PR China.
authors.forename: Nimaichand
authors.initials: N
authors.lastname: Salam
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences,Urumqi, 830011,PR China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Guo
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091,PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences,Urumqi, 830011,PR China.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275,PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li