title: Anti-diabetic activity of recombinant irisin in STZ-induced insulin-deficient diabetic mice.
abstract: In order to investigate the hypoglycemic effects and potential mechanism of recombinant irisin on diabetes, STZ-induced diabetic mice were established and treated with irisin. The results showed that daily water and food intake, and blood glucose significantly decreased after various concentrations of recombinant irisin treatment by intraperitoneal injection, of which 1.0 mg/kg was the optimal dose for lowering blood glucose. However, the body weight exhibited no significant difference during the treatment within groups, although the 0.9% NaCl treated group showed a trend of decreased body weight and the irisin treated groups showed a tendency of increasing weight. The oral glucose tolerance was improved, and serum insulin and circulating irisin content were significantly elevated in diabetic mice after 1.0 mg/kg irisin-injection treatment, compared to diabetic mice treated with 0.9% NaCl. 1.0 mg/kg irisin-injection also significantly increased the expression of energy and metabolism-related genes. In addition, oral administration of irisin lowered the blood glucose in diabetic mice. Our data suggested that irisin could lower blood glucose in insulin-deficient diabetic mice, to some extent, through irisin-mediated induction of energy and metabolic genes expression. These observations laid a foundation for the development of irisin-based therapy.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Huikun
authors.initials: H
authors.lastname: Duan
authors.affiliation: Tianjin Children's Hospital, 300074 Tianjin, China.
authors.forename: Baicheng
authors.initials: B
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Haisong
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Zaizhong
authors.initials: Z
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Pingzhe
authors.initials: P
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Umar
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, China; Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, 300071 Tianjin, China. Electronic address: mgl@nankai.edu.cn.
authors.forename: Minggang
authors.initials: M
authors.lastname: Li
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005353:Fibronectins / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D006442:Glycated Hemoglobin / Q000378:metabolism; D007328:Insulin / Q000097:blood / Q000378:metabolism; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins
chemical_list: D001786:Blood Glucose; D005353:Fibronectins; D006442:Glycated Hemoglobin; D007328:Insulin; D011994:Recombinant Proteins; C571428:FNDC5 protein, mouse
keywords: Diabetic mice; Hypoglycemic effects; Recombinant irisin