title: [Algicidal effect of (2-isobutoxyphenyl) amine on Alexandrium tamarense].
abstract: OBJECTIVE
  A strain named BS01 showed strong algicidal activity to Alexandrium tamarense and we got algicidal compound (2-isobutoxyphenyl) amine from BS01 to study its algicidal effect on A. tamarense.
  
  
  METHODS
  We studied the algicidal mechanism of (2-isobutoxyphenyl) amine on photosynthetic process, antioxidant enzyme activities and morphological change of A. tamarense.
  
  
  RESULTS
  After 24 hours treatment with (2-isobutoxyphenyl) amine, algicidal activity was 84. 1% with the concentration of 20 µg/mL. The compound could induce a reactive oxygen species burst in P. globosa in 0. 5 hours which could cause serious oxidative damage to algal cells. The Fv/Fm value which could reflect photosystem II (PS II) electron flow status also decreased. To eliminate the excess ROS, the activities of the antioxidant systems (including superoxide dismutase and catalase) increased significantly during exposure. Transmission electron microscope analysis showed obvious morphological modifications of chloroplast dismantling as a part of the algicidal process.
  
  
  CONCLUSIONS
  These results indicated that the lysis mechanism of algicidal compound on algae may primarily be the increasing level of ROS in the algal cells.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Peng
authors.forename: Su
authors.initials: S
authors.lastname: Zhang
authors.forename: Xinli
authors.initials: X
authors.lastname: An
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
mesh_terms: D000588:Amines / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004141:Dinoflagellida / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010788:Photosynthesis; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: C000604854:(2-isobutoxyphenyl)amine; D000588:Amines; D000975:Antioxidants; D015800:Protozoan Proteins; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase