title: ALX1 promotes migration and invasion of lung cancer cells through increasing snail expression.
abstract: Lung cancer is one of the main causes in cancer-related death. Here we reported a novel functional role of Aristaless-like homeobox1 (ALX1) in lung carcinogenesis. Analysis of ALX1 in lung cancer specimens confirms upregulation of ALX1 in lung cancer, especially these with distant metastasis. Moreover, higher level of ALX1 expression is associated with poorer prognosis of lung cancer patients. Ectopic expression of ALX1 significantly promotes lung cancer cell proliferation, migration and invasion, while ALX1 silencing by siRNA significantly inhibits these abilities of lung cancer cells. The functional role of ALX1 is dependent on increasing Snail expression and knockdown of Snail could restrain the role of ALX1. Collectively, we identify critical roles of ALX1 in lung cancer development and progression. These findings may serve as a framework for future investigations designed to more comprehensive determination of ALX1 as a potential therapeutic target.
authors.affiliation: Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Dalian Medical University, Graduate School of Dalian Medical University Dalian 116027, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.affiliation: The First Affiliated Hospital of Dalian Medical University Dalian 116011, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, The Second Affiliated Hospital of Dalian Medical University Dalian 116027, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, The Second Affiliated Hospital of Dalian Medical University Dalian 116027, China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, The Second Affiliated Hospital of Dalian Medical University Dalian 116027, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital of Dalian Medical University Dalian 116011, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
authors.affiliation: The First Affiliated Hospital of Dalian Medical University Dalian 116011, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Radiation Oncology, The Second Affiliated Hospital of Dalian Medical University Dalian 116027, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zou
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology; D016133:Polymerase Chain Reaction; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: C489985:ALX1 protein, human; D018398:Homeodomain Proteins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors
keywords: Aristaless-like homeobox1; EMT; lung cancer; snail