title: All-in-One azides: empowered click reaction for in vivo labeling and imaging of biomolecules.
abstract: We designed and synthesized All-in-One (AIO) reactive azide reagents for bioorthogonal reactions with highly efficient Cu(I) ligand moieties, an azido group, and functional tags for imaging or purification. The AIO reagents displayed fast and efficient click ligation and can be applied in a wide range of in vivo systems.
authors.affiliation: College of Life Sciences, Beijing Normal University, Beijing 100875, China and National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Su
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China and National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn and Collaborative Innovation Center for Cancer Medicine, Beijing, 102206, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D001386:Azides / Q000138:chemical synthesis* / Q000737:chemistry; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry*; D003952:Diagnostic Imaging; D007202:Indicators and Reagents / Q000737:chemistry*; D008024:Ligands
chemical_list: D001386:Azides; D007202:Indicators and Reagents; D008024:Ligands; D003300:Copper