title: Doxorubicin nanobubble for combining ultrasonography and targeted chemotherapy of rabbit with VX2 liver tumor.
abstract: A new class of multifunctional nanobubble using poly(lactic-co-glycolic acid) (PLGA) has been developed as ultrasound imaging contrast agents, doxorubicin carriers, and enhancers of ultrasound-mediated drug delivery. The doxorubicin nanobubble (DOX-NB) wrapping carbon tetrafluoride gas was prepared with double emulsion method. We evaluated the enhanced ultrasonic function of the DOX-NB in vivo; its antitumor function was confirmed. The diameter of the prepared bubble was 500 nm, and the potential was -23 mV. The drug loading and encapsulation efficiency of the bubble were 78.6 and 7.4 %, respectively. Therefore, the DOX-NB greatly enhanced ultrasound imaging in vivo. Ultrasound combined with DOX-NB had significant antitumor effect. Compared with other groups, the tumor growth rate and the proliferation index were the lowest while the survival rate and apoptosis index were the highest.
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Meng
authors.affiliation: The Department of Pathology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: The Department of Radiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Chongchong
authors.initials: C
authors.lastname: Wu
authors.affiliation: The Department of Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Department of Critical Care Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zang
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Xiangchun
authors.initials: X
authors.lastname: Lin
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Canghai
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Su
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Kuiliang
authors.initials: K
authors.lastname: Liu
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China.
authors.forename: Yadan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China. xinyingxue2010@163.com.
authors.forename: Xinying
authors.initials: X
authors.lastname: Xue
authors.affiliation: The Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, 100038, China. wujing36@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011817:Rabbits; D014407:Tumor Cells, Cultured; D014465:Ultrasonics; D023041:Xenograft Model Antitumor Assays
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D004317:Doxorubicin
keywords: Doxorubicin nanobubble (DOX-NB); Treatment; Ultrasound; VX2 liver tumor