title: Activation of Antigen-Specific CD8(+) T Cells by Poly-DL-Lactide/Glycolide (PLGA) Nanoparticle-Primed Gr-1(high) Cells.
abstract: OBJECTIVE
  The aim of this study was to investigate the induction of antigen-specific T cell activation and cell cycle modulation by a poly-DL-lactide/glycolide (PLGA) nanoparticle (NP)-primed CD11b(+)Gr-1(high) subset isolated from mouse bone marrow.
  
  
  METHODS
  PLGA NPs containing the ovalbumin (OVA) antigen were prepared using the double emulsion and solvent evaporation method, and protein release rate and cell viability were determined. The Lin2(¯)CD11b(+)Gr-1(high)Ly6c(low) (Gr-1(high)) subset was sorted from the bone marrow of C57BL/6 J mice by fluorescence-activated cell sorting (FACS) and co-cultured with OT-I CD8(+) splenic T cells. Proliferation of OT-I CD8(+) T cells was monitored, and cell cycles were determined by 5-bromo-2'-deoxyuridine (BrdU) labeling.
  
  
  RESULTS
  Treatment of Gr-1(high) cells with PLGA/OVA NPs upregulated expression of the SIINFEKL-H2K(b) complex in the context of MHC I. Co-cultures of OT-I CD8(+) T cells with the PLGA/OVA NP-primed Gr-1(high) cells induced the proliferation of T cells in vitro and modulated cell division and morphology. Treatment of Gr-1(high) cells with PLGA/OVA NPs also induced cell apoptosis and necrosis.
  
  
  CONCLUSIONS
  This study demonstrated the function of PLGA/OVA NPs in the activation of OT-I CD8(+) T cells and the capability of cross-presentation via the Gr-1(high) polymorphonuclear subset from mouse bone marrow.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, 33 Linsen South Road, Taipei City, 10050, Taiwan.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Luo
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, 33 Linsen South Road, Taipei City, 10050, Taiwan. ywyang@ntu.edu.tw.
authors.forename: Ya-Wun
authors.initials: YW
authors.lastname: Yang
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D039481:CD11b Antigen / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002453:Cell Cycle; D002478:Cells, Cultured; D018920:Coculture Techniques; D007114:Immunization; D019344:Lactic Acid / Q000737:chemistry*; D008213:Lymphocyte Activation; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011956:Receptors, Cell Surface / Q000276:immunology*
chemical_list: D039481:CD11b Antigen; D011956:Receptors, Cell Surface; C551746:granulocyte receptor 1, mouse; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D010047:Ovalbumin
keywords: antigen presentation; nanoparticles; poly(lactide-co-glycolide); polymorphonuclear cells