title: The Sub1 nuclear protein protects DNA from oxidative damage.
abstract: Reactive oxygen species are a by-product of aerobic metabolism that can damage lipid, proteins, and nucleic acids. Oxidative damage to DNA is especially critical, because it can lead to cell death or mutagenesis. Previously we reported that the yeast sub1 deletion mutant is sensitive to hydrogen peroxide treatment and that the human SUB1 can complement the sensitivity of the yeast sub1 mutant. In this study, we find that Sub1 protects DNA from oxidative damage in vivo and in vitro. We demonstrate that transcription of SUB1 mRNA is induced by oxidative stress and that the sub1Δ mutant has an increased number of chromosomal DNA strand breaks after peroxide treatment. We further demonstrate that purified Sub1 protein can protect DNA from oxidative damage in vitro, using the metal ion catalyzed oxidation assay.
authors.affiliation: Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA, 01655, USA.
authors.forename: Lijian
authors.initials: L
authors.lastname: Yu
authors.affiliation: Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA, 01655, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.affiliation: The Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Xincai
authors.initials: X
authors.lastname: Ji
authors.affiliation: Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA, 01655, USA. Michael.Volkert@umassmed.edu.
authors.forename: Michael R
authors.identifier: http://orcid.org/0000-0002-0045-0912
authors.initials: MR
authors.lastname: Volkert
mesh_terms: D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000502:physiology*; D006801:Humans; D018384:Oxidative Stress; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; C509201:SUB1 protein, human; D014157:Transcription Factors
keywords: Oxidation resistance; Oxidative damage; Oxidative stress; Reactive oxygen species; Sub1 protein