title: Zebrafish Plzf transcription factors enhance early type I IFN response induced by two non-enveloped RNA viruses.
abstract: The BTB-POZ transcription factor Promyelocytic Leukemia Zinc Finger (PLZF, or ZBTB16) has been recently identified as a major factor regulating the induction of a subset of Interferon stimulated genes in human and mouse. We show that the two co-orthologues of PLZF found in zebrafish show distinct expression patterns, especially in larvae. Although zbtb16a/plzfa and zbtb16b/plzfb are not modulated by IFN produced during viral infection, their over-expression increases the level of the early type I IFN response, at a critical phase in the race between the virus and the host response. The effect of Plzfb on IFN induction was also detectable after cell infection by different non-enveloped RNA viruses, but not after infection by the rhabdovirus SVCV. Our findings indicate that plzf implication in the regulation of type I IFN responses is conserved across vertebrates, but at multiple levels of the pathway and through different mechanisms.
authors.affiliation: INRA, Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas, France.
authors.forename: E
authors.initials: E
authors.lastname: Aleksejeva
authors.affiliation: INRA, Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas, France.
authors.forename: A
authors.initials: A
authors.lastname: Houel
authors.affiliation: Institut Pasteur, Unité Macrophages et Développement de l'Immunité, 25-28 rue du Docteur Roux, F-75015 Paris, France; CNRS, URA 2578, F-75015 Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Briolat
authors.affiliation: Institut Pasteur, Unité Macrophages et Développement de l'Immunité, 25-28 rue du Docteur Roux, F-75015 Paris, France; CNRS, URA 2578, F-75015 Paris, France.
authors.forename: J-P
authors.initials: JP
authors.lastname: Levraud
authors.affiliation: INRA, Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Langevin
authors.affiliation: INRA, Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas, France. Electronic address: Pierre.Boudinot@jouy.inra.fr.
authors.forename: P
authors.initials: P
authors.lastname: Boudinot
mesh_terms: D000818:Animals; D006801:Humans; D007113:Immunity, Innate; D007370:Interferon Type I / Q000276:immunology* / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010802:Phylogeny; D011070:Poly I-C / Q000276:immunology; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D012327:RNA Virus Infections / Q000276:immunology*; D012328:RNA Viruses / Q000276:immunology*; D012367:RNA, Viral / Q000276:immunology; D059467:Transcriptome; D015027:Zebrafish / Q000235:genetics / Q000276:immunology*; D029961:Zebrafish Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D007370:Interferon Type I; D051741:Kruppel-Like Transcription Factors; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D012367:RNA, Viral; C513267:Zbtb16 protein, mouse; D029961:Zebrafish Proteins; C000611657:zbtb16a protein, zebrafish; C092037:ZBTB16 protein, human; D011070:Poly I-C
keywords: Antiviral innate immunity; Interferon; PLZF; ZBTB; Zebrafish