title: [Inhibitory effect of Genipin on uncoupling protein-2 and energy metabolism of androgen-independent prostate cancer cells].
abstract: OBJECTIVE
  To explore whether the inhibitory effect of Genipin on uncoupling protein-2 (UCP-2) in mitochondria is involved in energy metabolism of androgen-independent PC3 prostate cancer cells.
  
  
  METHODS
  PC3 prostate cancer cells were cultured and treated with Genipin at the concentrations of 40, 80, and 160 μmol/L for 48 hours. Then the proliferation of the cells was detected by MTT assay, the expression of UCP-2 mRNA determined by RT-PCR, and the content of intracellular pyruvic acid (PA) and the activity of succinate dehydrogenase (SDH) in the mitochondria measured by visible spectrophotometry.
  
  
  RESULTS
  With the increased concentration of Genipin, the proliferative activity of the PC-3 cells, the expression level of UCP-2 mRNA, the content of intracellular PA and the activity of SDH in the cells were all decreased, namely, with the enhanced inhibitory effect of Genipin on UCP-2, a trend of reduction was observed in the proliferation of the cells, intracellular PA content, and SDH activity in the mitochondria.
  
  
  CONCLUSIONS
  Genipin is involved in the energy metabolism of androgen-independent PC3 prostate cancer cells by reducing the content of intracellular PA and the activity of SDH in the mitochondria, which may be associated with its inhibitory effect on UCP-2.
authors.forename: Mao-liang
authors.initials: ML
authors.lastname: Yao
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
authors.forename: Yong-chun
authors.initials: YC
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Zhu
authors.forename: Qing-tao
authors.initials: QT
authors.lastname: Yang
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Xia
mesh_terms: D045744:Cell Line, Tumor / Q000187:drug effects; D004734:Energy Metabolism; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D039823:Iridoids / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D012333:RNA, Messenger; D013385:Succinate Dehydrogenase / Q000378:metabolism; D000071246:Uncoupling Protein 2
chemical_list: D007473:Ion Channels; D039823:Iridoids; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C491545:SDHD protein, human; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2; D019289:Pyruvic Acid; C007834:genipin; D013385:Succinate Dehydrogenase