title: Characterization of a novel single-stranded RNA virus, closely related to fusariviruses, infecting the plant pathogenic fungus Alternaria brassicicola.
abstract: The alternaria blackspot of rapeseed is one of the most prominent diseases of rapeseed. It is caused by three species of the genus Alternaria: Alternaria brassicicola, Alternaria brassicae, and Alternaria raphanin. Here we report a novel positive-sense RNA virus from an A. brassicicola strain 817-14. The virus has a 6639 nucleotide (nt) long genome, excluding a poly (A)-tail, and was predicted to contain three putative open reading frames (ORF1, ORF2, and ORF3). The large ORF1 encoded a 174-kDa polyprotein (composed of 1522 amino acid residues) containing a conserved RNA-dependent RNA polymerase (RdRp) domain and a helicase domain. The other two smaller ORFs encoded polypeptides with unknown function. Homology search and phylogenetic analysis, based on the RdRp and helicase domains, suggest that this virus is related to and grouped with Sclerotinia sclerotiorum fusarivirus 1 (SsFV1), Rosellinia necatrix fusarivirus 1 (RnFV1), Fusarium graminearum virus-DK21 (FgV1), and Penicillium roqueforti RNA mycovirus 1 (PrRV1), all of which belong to a newly proposed family Fusariviridae. For this study, we designed the virus as "Alternaria brassicicola fusarivirus 1" (AbFV1). Virus elimination revealed that AbFV1 has no conspicuous impact on the biological properties of its host.
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province 410128, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province 410128, PR China.
authors.forename: Hong Hong
authors.initials: HH
authors.lastname: Shang
authors.affiliation: Changde Agriculture & Science Research Institute, Changde City, Hunan Province 415000, PR China.
authors.forename: Chuan Xia
authors.initials: CX
authors.lastname: Zhu
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province 410128, PR China; Changde Agriculture & Science Research Institute, Changde City, Hunan Province 415000, PR China.
authors.forename: Jun Zi
authors.initials: JZ
authors.lastname: Zhu
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province 410128, PR China; Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, PR China.
authors.forename: Hong Jian
authors.initials: HJ
authors.lastname: Zhu
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province 410128, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province 410128, PR China. Electronic address: bdgao@aliyun.com.
authors.forename: Bi Da
authors.initials: BD
authors.lastname: Gao
mesh_terms: D000528:Alternaria / Q000821:virology*; D000067148:Fungal Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral
keywords: Fusariviridae; Mycovirus; Single-stranded RNA virus