title: Effect of miR-340 on gastric cancer cell proliferation and apoptosis.
abstract: Gastric cancer pathogenesis is a multi-factor, multi-step, complicated process that related to gene abnormal expression. This study intended to explore the miR-340 effect on human gastric cancer cell line SGC-7901 and BGG823 proliferation and apoptosis, as to provide theoretical basis and experimental evidence for potential clinical application. Array was used to screen gastric cancer related abnormal genes. Q-PCR was applied to detect the screened genes expression in tissue and gastric cancer cells. MTT and colony formation assay were performed to evaluate miR-340 impact on gastric cancer proliferation. Flow cytometry was used to determine cell cycle and cell apoptosis. Q-PCR showed that miR-340 overexpressed in gastric cancer tissue significantly compared with normal control (P < 0.01). MiR-340 overexpression can promote SGC-7901 and BGC823 cells proliferation with 50% proliferation rate. Soft agar colony formation assay also showed that miR-340 overexpression can facilitate gastric cancer cell proliferation. Cell cycle analysis revealed that miR-340 overexpression can reduce cell apoptosis. Annexin V/PI staining demonstrated that miR-340 transfection can decrease cell apoptotic rate (4.58%, 1.98%, 2.11%). MiR-340 can promote tumor cell growth and reduce cell apoptosis effectively.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Haiquan
authors.initials: H
authors.lastname: Qiao
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection
chemical_list: C570046:MIRN340 microRNA, human; D035683:MicroRNAs
keywords: apoptosis; gastric cancer; miR-340; proliferation