title: Successive pretreatment and enzymatic saccharification of sugarcane bagasse in a packed bed flow-through column reactor aiming to support biorefineries.
abstract: A packed bed flow-through column reactor (PBFTCR) was used for pretreatment and subsequent enzymatic hydrolysis of sugarcane bagasse (SCB). Alkaline pretreatment was performed at 70 °C for 4h with fresh 0.3M NaOH solution or with liquor recycled from a previous pretreatment batch. Scheffersomyces stipitis NRRL-Y7124 was used for fermentation of sugars released after enzymatic hydrolysis (20 FPU g(-1) of dry SCB). The highest results for lignin removal were 61% and 52%, respectively, observed when using fresh NaOH or the first reuse of the liquor. About 50% of cellulosic and 57% of hemicellulosic fractions of pretreated SCBs were enzymatically hydrolyzed and the maximum ethanol production was 23.4 g L(-1) (ethanol yield of 0.4 gp gs(-1)), with near complete consumption of both pentoses and hexoses present in the hydrolysate during the fermentation. PBFTCR as a new alternative for SCB-biorefineries is presented, mainly considering its simple configuration and efficiency for operating with a high solid:liquid ratio.
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP 12602-810, Lorena, São Paulo, Brazil. Electronic address: teran-2017@usp.br.
authors.forename: R
authors.initials: R
authors.lastname: Terán-Hilares
authors.affiliation: Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional, CP 07738 Distrito Federal, Mexico.
authors.forename: A L
authors.initials: AL
authors.lastname: Reséndiz
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional, CP 07738 Distrito Federal, Mexico.
authors.forename: R T
authors.initials: RT
authors.lastname: Martínez
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP 12602-810, Lorena, São Paulo, Brazil.
authors.forename: S S
authors.initials: SS
authors.lastname: Silva
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP 12602-810, Lorena, São Paulo, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Santos
mesh_terms: D056804:Biofuels; D002482:Cellulose / Q000378:metabolism* / Q000648:ultrastructure; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D004718:Saccharomycetales / Q000378:metabolism*; D031786:Saccharum
chemical_list: D056804:Biofuels; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse
keywords: Bioethanol production; Enzymatic saccharification; Packed bed reactor; Reuse of alkaline liquor; Sugarcane bagasse