title: Urinary Nucleic Acid TSPAN13-to-S100A9 Ratio as a Diagnostic Marker in Prostate Cancer.
abstract: The potential use of urinary nucleic acids as diagnostic markers in prostate cancer (PCa) was evaluated. Ninety-five urine samples and 234 prostate tissue samples from patients with PCa and benign prostatic hyperplasia (BPH) were analyzed. Micro-array analysis was used to identify candidate genes, which were verified by the two-gene expression ratio and validated in tissue mRNA and urinary nucleic acid cohorts. Real-time quantitative polymerase chain reaction (qPCR) was used to measure urinary nucleic acid levels and tissue mRNA expression. The TSPAN13-to-S100A9 ratio was selected to determine the diagnostic value of urinary nucleic acids in PCa (P = 0.037) and shown to be significantly higher in PCa than in BPH in the mRNA and nucleic acid cohort analyses (P < 0.001 and P = 0.013, respectively). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0.898 and 0.676 in tissue mRNA cohort and urinary nucleic acid cohort, respectively. The TSPAN13-to-S100A9 ratio showed a strong potential as a diagnostic marker for PCa. The present results suggest that the analysis of urine supernatant can be used as a simple diagnostic method for PCa that can be adapted to the clinical setting in the future.
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Chunri
authors.identifier: 0000-0002-3043-6687
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Ye-Hwan
authors.identifier: 0000-0002-8676-7119
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Ho Won
authors.identifier: 0000-0002-8164-4427
authors.initials: HW
authors.lastname: Kang
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Sung Phil
authors.initials: SP
authors.lastname: Seo
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Pildu
authors.initials: P
authors.lastname: Jeong
authors.affiliation: Bio-Medical Science Co. Ltd, Daejeon, Korea.
authors.forename: Il-Seok
authors.initials: IS
authors.lastname: Lee
authors.affiliation: Bio-Medical Science Co. Ltd, Daejeon, Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Kim
authors.affiliation: Nucleic Acid Research Center, Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon, Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Dong-Eui University, Busan, Korea.
authors.forename: Yung Hyun
authors.identifier: 0000-0002-1454-3124
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Food and Biotechnology, Chung-Ang University, Seoul, Korea.
authors.forename: Sung-Kwon
authors.identifier: 0000-0002-4514-3457
authors.initials: SK
authors.lastname: Moon
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Seok Joong
authors.identifier: 0000-0001-7737-4746
authors.initials: SJ
authors.lastname: Yun
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Wun-Jae
authors.identifier: 0000-0002-8060-8926
authors.initials: WJ
authors.lastname: Kim
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000652:urine*; D040502:Calgranulin B / Q000235:genetics*; D015331:Cohort Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009696:Nucleic Acids / Q000235:genetics* / Q000652:urine*; D020411:Oligonucleotide Array Sequence Analysis; D011467:Prostate / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000235:genetics / Q000652:urine; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000652:urine*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D059470:Tetraspanins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D040502:Calgranulin B; D009696:Nucleic Acids; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C502874:TSPAN13 protein, human; D059470:Tetraspanins
keywords: Cell-free Nucleic Acid; Diagnostic Marker; Prostatic Neoplasms; S100A9; TSPAN13; Two-gene Expression Ratio; Urine