title: miR-4299 mediates the invasive properties and tumorigenicity of human follicular thyroid carcinoma by targeting ST6GALNAC4.
abstract: Altered sialylation is closely associated with tumor progression and invasiveness. Micro-RNAs endogenous regulators of gene expression have been implicated in human thyroid carcinoma invasiveness. The objective of this study is to examine the alterations of miR-4299 and ST6GALNAC family in human follicular thyroid carcinoma during metastatic process. qRT-PCR showed the differential expressional profiles of miR-4299 and ST6GALNAC family in three kinds of thyroid cell lines (FTC-133,FTC-238, Nthy-ori 3-1) and clinical tissue specimens(malignant and borderline). The altered expression levels of ST6GALNAC4 were corresponding to invasive phenotypes of FTC-133 and FTC-238 cells both in vitro and in vivo. Further date indicated that miR-4299 regulated tumor progression and invasiveness by directly targeting ST6GALNAC4. This study implies the potential therapeutic application of miR-4299 and ST6GALNAC4 in modulating the invasion and tumorigenicity of follicular thyroid carcinoma cell.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Liaoning Province, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Miao
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Microbiology, Dalian Medical University, Dalian, Liaoning Province, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Liaoning Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Liaoning Province, China.
authors.forename: Jinchao
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning Province, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning Province, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Liaoning Province, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics* / Q000473:pathology; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D012799:Sialyltransferases / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C000611725:MIR4299 microRNA, human; D035683:MicroRNAs; D012799:Sialyltransferases; C485573:ST6GALNAC4 protein, human
keywords: ST6GALNAC family; human follicular thyroid carcinoma cell line; invasive properties; miR-4299; tumorigenicity