title: Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14.
abstract: Bacterial populations differentiate at the subspecies level into clonal complexes. Intraclonal genome diversity was studied in 100 isolates of the two dominant Pseudomonas aeruginosa clones C and PA14 collected from the inanimate environment, acute and chronic infections. The core genome was highly conserved among clone members with a median pairwise within-clone single nucleotide sequence diversity of 8 × 10(-6) for clone C and 2 × 10(-5) for clone PA14. The composition of the accessory genome was, on the other hand, as variable within the clone as between unrelated clones. Each strain carried a large cargo of unique genes. The two dominant worldwide distributed P. aeruginosa clones combine an almost invariant core with the flexible gain and loss of genetic elements that spread by horizontal transfer.
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Fischer
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Klockgether
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Patricia
authors.initials: P
authors.lastname: Morán Losada
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Philippe
authors.initials: P
authors.lastname: Chouvarine
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Cramer
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Colin F
authors.initials: CF
authors.lastname: Davenport
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Dethlefsen
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Marie
authors.initials: M
authors.lastname: Dorda
authors.affiliation: Bioinformatics and Systems Biology, Justus-Liebig-Universität, Gießen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.affiliation: Bioinformatics and Systems Biology, Justus-Liebig-Universität, Gießen, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Hilker
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Samira
authors.initials: S
authors.lastname: Mielke
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Torben
authors.initials: T
authors.lastname: Schönfelder
authors.affiliation: Institute for Medical Microbiology and Hospital Epidemiology, OE 5210, Hannover Medical School, Hannover, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Suerbaum
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Türk
authors.affiliation: Institute for Medical Microbiology and Hospital Epidemiology, OE 5210, Hannover Medical School, Hannover, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Woltemate
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Wiehlmann
authors.affiliation: Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.|Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), German Center for Lung Research, Hannover, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Tümmler
mesh_terms: D017124:Conserved Sequence; D004783:Environmental Microbiology; D022761:Gene Transfer, Horizontal; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification