title: Thrombospondin-1 differentially regulates microRNAs in vascular smooth muscle cells.
abstract: Thrombospondin-1 (TSP-1) is an important regulator of vascular smooth muscle cell (VSMC) physiology and gene expression. MicroRNAs (microRNA), small molecules that regulate protein translation, have emerged as potent regulators of cell function. MicroRNAs have been shown to be involved in intimal hyperplasia, atherosclerosis, and upregulated in the vasculature in diabetes. The purpose of this study was to identify microRNAs regulated by TSP-1 in vascular smooth muscle cells (VSMCs). Human VSMCs were treated for 6 h with basal media or TSP-1 both supplemented with 0.2% FBS. Cells were then snap frozen and RNA extracted. An Affymetrix GeneChip microRNA array analysis was performed in triplicate on three separate collections. Confirmatory qrtPCR was performed. Data were analyzed by ANOVA or t test, with significance set at p < 0.05. MicroRNAs identified were subjected to KEGG pathway analysis using the DIANA tools miRPath online tool. TSP-1 upregulated 22 microRNAs and downregulated 18 microRNAs in VSMCs (p < 0.05). The most upregulated microRNA was miR-512-3p (45.12 fold). The microRNA most downregulated by TSP-1 was miR-25-5p, which was decreased by 9.61. Of note, five members of the mir-17-92 cluster were downregulated. KEGG analysis revealed that thirty-three cellular signaling pathways were impacted by these microRNAs and that nine pathways were relevant to vascular disease. MicroRNAs regulate protein expression at the level of translation and may represent a significant mechanism by which TSP-1 regulates VSMC function. Several of the microRNAs identified have a role in vascular function. The miR-17-92 cluster family, which was found to exhibit reduced expression in this study, is known to be involved in angiogenesis and vascular function. TSP-1 regulates multiple microRNAs in VSMCs adding a new layer of complexity to TSP-1 regulation of VSMC function.
authors.affiliation: Division of Vascular Surgery and Endovascular Services, SUNY Upstate Medical University College of Medicine, 750 East Adams Street, Syracuse, NY, 13210, USA. maierk@upstate.edu.|Department of Veterans Affairs VA Healthcare Network Upstate New York at Syracuse, Syracuse, USA. maierk@upstate.edu.
authors.forename: Kristopher G
authors.initials: KG
authors.lastname: Maier
authors.affiliation: Division of Vascular Surgery and Endovascular Services, SUNY Upstate Medical University College of Medicine, 750 East Adams Street, Syracuse, NY, 13210, USA.|Department of Veterans Affairs VA Healthcare Network Upstate New York at Syracuse, Syracuse, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Ruhle
authors.affiliation: Division of Vascular Surgery and Endovascular Services, SUNY Upstate Medical University College of Medicine, 750 East Adams Street, Syracuse, NY, 13210, USA.|Department of Veterans Affairs VA Healthcare Network Upstate New York at Syracuse, Syracuse, USA.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Stein
authors.affiliation: Department of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, USA.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Gentile
authors.affiliation: Department of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, USA.
authors.forename: Frank A
authors.initials: FA
authors.lastname: Middleton
authors.affiliation: Division of Vascular Surgery and Endovascular Services, SUNY Upstate Medical University College of Medicine, 750 East Adams Street, Syracuse, NY, 13210, USA.|Department of Veterans Affairs VA Healthcare Network Upstate New York at Syracuse, Syracuse, USA.
authors.forename: Vivian
authors.initials: V
authors.lastname: Gahtan
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D019700:Thrombospondin 1 / Q000502:physiology*
chemical_list: D035683:MicroRNAs; D019700:Thrombospondin 1
keywords: Gene expression profiling; MicroRNA; Thrombospondins; Vascular smooth muscle