title: Porous CS based membranes with improved antimicrobial properties for the treatment of infected wound in veterinary applications.
abstract: Recently, much attention has been given to the use of innovative solution for the treatment of infected wounds in animals. Current applied treatments are often un-effective leading to infection propagation and animal death. Novel engineered membranes based on chitosan (CS) can be prepared to combine local antimicrobial effect, high flexibility and easy manipulation. In this work, CS crosslinked porous membranes with improved antimicrobial properties were prepared via freeze-drying technique to promote wound healing and to reduce the bacterial proliferation in infected injuries. Silver nanoparticles (AgNPs) and gentamicin sulfate (GS) were incorporated into the CS matrices to impart antibacterial properties on a wild range of strains. CS based porous membranes were tested for their physicochemical, thermal, mechanical as well as swelling and degradation behavior at physiological condition. Additionally, GS release profile was investigated, showing a moderate burst effect in the first days followed by a decreasing release rate which it was maintained for at least 56 days. Moreover, porous membranes loaded with GS or AgNPs showed good bactericidal activity against both of Gram-positive and Gram-negative bacteria. The bacterial strains used in this work were collected in chelonians after carapace injuries to better mimic the environment after trauma.
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Turin, Italy. Electronic address: chiara.tondaturo@polito.it.
authors.forename: C
authors.initials: C
authors.lastname: Tonda-Turo
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Turin, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ruini
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Turin, Italy; Clinic for Exotic Animals, CVS, Via Sandro Giovannini 53, 00137 Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Argentati
authors.affiliation: Clinic for Exotic Animals, CVS, Via Sandro Giovannini 53, 00137 Rome, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Di Girolamo
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Turin, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Robino
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Turin, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Nebbia
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Turin, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ciardelli
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry*; D005839:Gentamicins / Q000737:chemistry / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D005839:Gentamicins; D012834:Silver; D048271:Chitosan
keywords: Antibacterial agent; Chitosan; Gentamicin; Silver nanoparticles; Wound healing