title: Spectrum of EGFR gene mutations in Vietnamese patients with non-small cell lung cancer.
abstract: OBJECTIVE
  Epidermal growth factor receptor (EGFR) mutational status is a crucial biomarker for prediction of response to tyrosine kinase inhibitors in patients with non-small cell lung cancer (NSCLC). Although these mutations have been well characterized in other countries, little is known about the frequency or spectrum of EGFR mutations in Vietnamese NSCLC patients.
  
  
  METHODS
  Using Sanger DNA sequencing, we investigated mutations in EGFR exons 18-21 from 332 patients diagnosed with NSCLC at University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam. DNA was extracted from formalin-fixed, paraffin-embedded tissues, followed by PCR amplification and sequencing.
  
  
  RESULTS
  EGFR mutations were detected in 135 samples (40.7%), of which eight samples carried double mutations. In total, 46 different types of EGFR mutations were found, including six novel mutations (p.K713E, p.K714R, p.P794S, p.R803W, p.P848S, and p.K867E). Among the four exons investigated, exon 19 was most frequently mutated (63 out of 332 patients, 19%), with the p.E746_A750del appearing in 43 samples. Exon 21 was mutated in 56 samples (16.9%), of which 47 were p.L858R. Each of exons 18 and 20 was mutated in 12 samples (3.6%). The frequency of EGFR mutations was higher in females than in males (48.9% vs 35%, P = 0.012), but not statistically different between adenocarcinomas and other histological types of NSCLC (41.3% vs 34.5%, P = 0.478).
  
  
  CONCLUSIONS
  DNA sequencing detected EGFR mutations with high frequency and revealed a broad spectrum of mutation type in Vietnamese patients with NSCLC.
authors.affiliation: Center for Molecular Biomedicine and, Departments of.
authors.forename: Hoang Anh
authors.initials: HA
authors.lastname: Vu
authors.affiliation: Hematology and.
authors.forename: Phan Thi
authors.initials: PT
authors.lastname: Xinh
authors.affiliation: Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
authors.forename: Hua Thi Ngoc
authors.initials: HT
authors.lastname: Ha
authors.affiliation: Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
authors.forename: Ngo Thi Tuyet
authors.initials: NT
authors.lastname: Hanh
authors.affiliation: Center for Molecular Biomedicine and, Departments of.
authors.forename: Nguyen Duc
authors.initials: ND
authors.lastname: Bach
authors.affiliation: Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
authors.forename: Doan Thi Phuong
authors.initials: DT
authors.lastname: Thao
authors.affiliation: Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
authors.forename: Ngo Quoc
authors.initials: NQ
authors.lastname: Dat
authors.affiliation: Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
authors.forename: Nguyen Sao
authors.initials: NS
authors.lastname: Trung
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; Vietnam; mutation; non-small cell lung cancer