title: Immobilization of serum albumin and peptide aptamer for EPC on polydopamine coated titanium surface for enhanced in-situ self-endothelialization.
abstract: Restenosis and thrombosis are two major complications associated with vascular stents and grafts. The homing of circulating endothelial progenitor cells (EPCs) onto implant surfaces brings a new strategy to solve these problems by accelerating self -endothelialization in situ. Peptide aptamers with high affinity and specific recognition of EPCs can be immobilized to capture EPCs from the circulating blood. In this study, a biotinylated peptide aptamer (TPSLEQRTVYAK-GGGC-K-Biotin) for EPC, and bovine serum albumin (BSA) were co-immobilized onto titanium surface through avidin-biotin recognition to endow the surface with specific affinity for EPC and anti-platelet adhesion properties. Quartz crystal microbalance with dissipation (QCM-D), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and water contact angle measuring were adopted for coating characterization. EPC affinity and hemocompatibility of the coating were also investigated in vitro. The results demonstrated that aptamer and BSA co-immobilized surface significantly reduced platelet adhesion and fibrinogen adsorption/activation. Besides, such functional surface could remarkably enhance EPC adhesion, without affecting the behavior of endothelial cells (ECs) and smooth muscle cells (SMCs) obviously. The result shows the possibility of utilizing such a multifunctional surface in cardiovascular implants.
authors.affiliation: Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu, 610031, China; RegeMed Lab of Tissue Engineering, Faculty of Life Science, Northwest University, Xi'an, 710069, China. Electronic address: 362947953@qq.com.
authors.forename: Zhuoyue
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Stomology, Anhui Medical University, Hefei, 230032, China.
authors.forename: Quanli
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu, 610031, China; College of Stomology, Anhui Medical University, Hefei, 230032, China.
authors.forename: Jialong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu, 610031, China.
authors.forename: Rifang
authors.initials: R
authors.lastname: Luo
authors.affiliation: Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu, 610031, China; Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany.
authors.forename: Manfred F
authors.initials: MF
authors.lastname: Maitz
authors.affiliation: Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu, 610031, China. Electronic address: huangnan1956@163.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000818:Animals; D052158:Aptamers, Peptide / Q000737:chemistry*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D007211:Indoles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D052158:Aptamers, Peptide; D020099:Coated Materials, Biocompatible; D007211:Indoles; D011108:Polymers; D012709:Serum Albumin; C568283:polydopamine; D014025:Titanium
keywords: Bovine serum albumin; In-situ self-endothelialization; Peptide aptamer; Surface modification; Titanium