title: Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.
abstract: Radiotherapy (RT) is one main method for the treatment of esophageal squamous cell carcinoma (ESCC), and the radioresistance is the predominant cause of patients with local recurrence. The previous results of gene microarray and subsequent verification showed that NRAGE might be involved in radiation resistance of ESCC cells. In this study, we reestablished human esophageal carcinoma radioresistant cell lines TE13R120 and ECA109R60 with gradient dose irradiation as previously reported, respectively. NRAGE expression was high in TE13R120 and ECA109R60 cells and was correlative with ionizing radiation (IR) resistance in clinic. However, the radiosensitivity of TE13R120 cells had a remarkable increase detected by colony formation assays after siRNA against NRAGE (siNRG) transfection into TE13R120 cells. Compared with TE13 cells, an increasing number of TE13R120 cells with NRAGE overexpression in S phase and a lower ratio in G2/M were observed by flow cytometry method (FCM). Intriguingly, the above changes were partially reversed in TE13R120 cells treated with siNRG. More importantly, the ectopic subcellular localization of NRAGE mediated nuclear translocation of β-catenin which may be one reason of IR resistance of esophageal carcinoma cell. These data indicate that NRAGE extremely may be a pivotal factor involved in Wnt/β-catenin signal pathway, mediating nuclear translocation of β-catenin and then facilitating the formation of radioresistance of ESCC.
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Huandi
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. xxy0636@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Xue
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Han
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000532:radiotherapy; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D011839:Radiation, Ionizing; D011879:Radiotherapy Dosage; D060449:Wnt Signaling Pathway / Q000235:genetics; D055815:Young Adult; D051176:beta Catenin / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; C120897:MAGED1 protein, human; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D051176:beta Catenin
keywords: Esophageal squamous cell carcinoma; NRAGE; Radioresistance; Subcellular localization; β-catenin