title: Pitfalls in the detection of CV2 (CRMP5) antibodies.
abstract: CV2 antibodies (CV2-ab) associate with paraneoplastic neurological syndromes (PNS) and small-cell lung cancer. This study was designed to assess the sensitivity of two widely used anti-CV2 commercial kits. Fifty three sera with CV2-ab identified by immunohistochemistry on paraformaldehyde-perfused rat brain were tested with two commercial immunoblot kits (Euroimmun AG, and Ravo Diagnostika) and 4 (7.5%) of them were negative with the commercial kits. The 4 samples were positive by immunofluorescence on HEK293 cells transfected with CRMP5 and immunoblot of these cells lysate. A few CV2-ab-positive sera may be missed by commercial immunoblots. Negative samples from patients with high suspicion for PNS should be tested by alternative methods.
authors.affiliation: Neuroimmunology Program, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: Sabater
authors.affiliation: Neuroimmunology Program, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Service of Neurology, Hospital Clinic, University of Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Saiz
authors.affiliation: Neuroimmunology Program, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain; Department of Neurology, University of Pennsylvania, Philadelphia, USA.
authors.forename: Josep
authors.initials: J
authors.lastname: Dalmau
authors.affiliation: Neuroimmunology Program, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Service of Neurology, Hospital Clinic, University of Barcelona, Spain. Electronic address: fgraus@clinic.ub.es.
authors.forename: Francesc
authors.initials: F
authors.lastname: Graus
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods / Q000592:standards; D057809:HEK293 Cells; D006801:Humans; D006867:Hydrolases; D015151:Immunoblotting / Q000379:methods / Q000592:standards; D008297:Male; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000097:blood* / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012189:Retrospective Studies
chemical_list: D001323:Autoantibodies; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C415087:DPYSL5 protein, human; D006867:Hydrolases
keywords: CV2 (CRMP5) antibodies; Cancer; Immunoblot; Paraneoplastic