title: Sevoflurane pretreatment enhance HIF-2α expression in mice after renal ischemia/reperfusion injury.
abstract: Ischemia/reperfusion (I/R) injury often occurs, which is one of the major causes of acute kidney injury, thus increasing in-hospital mortality. HIF-2α has a protective role against ischemia of the kidney. Renal ischemia/reperfusion under sevoflurane anesthesia resulted in drastic improvements in renal function. We hypothesized that underlying mechanism responsible for renal protection from sevoflurane pretreatment involves the upregulation of HIF-2α. Sevoflurane pretreatment were performed on WT and HIF-2α knockout mice before renal ischemia/reperfusion. Levels of blood urea nitrogen (BUN) and serum creatinine (Cr) were determined with a standard clinical automatic analyzer. The left kidneys were taken for morphological examination. Expression of HIF-2α in kidney tissue was examined by western blotting. In WT mice, group I/R injury had significantly higher BUN and Cr levels than group control, whereas group I/R + Sev had significantly lower BUN and Cr levels than group I/R injury. Renal HIF-2α expression levels were significantly higher in WT mice of group I/R + Sev than group control and group I/R. In HIF-2α(-/-) mice, group I/R + Sev showed much higher BUN and Cr levels and severer histological damage than group I/R and group control. Renal HIF-2α expression levels were significantly higher in WT mice of group I/R + Sev than group control and group I/R. Our findings suggested that HIF-2α might contribute to the beneficial effect of sevoflurane in renal ischemia/reperfusion injury.
authors.affiliation: Department of Anesthesia, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Beijie
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Department of Anesthesia, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Qionghui
authors.initials: Q
authors.lastname: Zhan
authors.affiliation: Department of Anesthesia, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Anesthesia, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Anesthesia, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Zhenzhou
authors.initials: Z
authors.lastname: He
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis*; D015153:Blotting, Western; D004195:Disease Models, Animal; D007677:Kidney Function Tests; D008738:Methyl Ethers / Q000494:pharmacology*; D051379:Mice; D018345:Mice, Knockout; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D000077149:Sevoflurane; D015854:Up-Regulation; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D000893:Anti-Inflammatory Agents; D051792:Basic Helix-Loop-Helix Proteins; D008738:Methyl Ethers; D000077149:Sevoflurane; D000099162:Endothelial PAS Domain-Containing Protein 1
keywords: HIF-2α; Sevoflurane; renal ischemia/reperfusion injury