title: Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp.
abstract: Paracoccidioides spp., a dimorphic pathogenic fungus, is the etiologic agent of paracoccidioidomycosis (PCM). PCM is an endemic disease that affects at least 10 million people in Latin America, causing severe public health problems. The drugs used against pathogenic fungi have various side effects and limited efficacy; therefore, there is an inevitable and urgent medical need for the development of new antifungal drugs. In the present study, we evaluated the transcriptional profile of Paracoccidioides lutzii exposed to argentilactone, a constituent of the essential oil of Hyptis ovalifolia. A total of 1,058 genes were identified, of which 208 were up-regulated and 850 were down-regulated. Cell rescue, defense and virulence, with a total of 26 genes, was a functional category with a large number of genes induced, including heat shock protein 90 (hsp90), cytochrome c peroxidase (ccp), the hemoglobin ligand RBT5 (rbt5) and superoxide dismutase (sod). Quantitative real-time PCR revealed an increase in the expression level of all of those genes. An enzymatic assay showed a significant increase in SOD activity. The reduced growth of Pbhsp90-aRNA, Pbccp-aRNA, Pbsod-aRNA and Pbrbt5-aRNA isolates in the presence of argentilactone indicates the importance of these genes in the response of Paracoccidioides spp. to argentilactone. The response of the P. lutzii cell wall to argentilactone treatment was also evaluated. The results showed that argentilactone caused a decrease in the levels of polymers in the cell wall. These results suggest that argentilactone is a potential candidate for antifungal therapy.
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Felipe Souto
authors.initials: FS
authors.lastname: Araújo
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Luciene Melo
authors.initials: LM
authors.lastname: Coelho
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Lívia do Carmo
authors.initials: Ldo C
authors.lastname: Silva
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Benedito Rodrigues
authors.initials: BR
authors.lastname: da Silva Neto
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Parente-Rocha
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Alexandre Melo
authors.initials: AM
authors.lastname: Bailão
authors.affiliation: Laboratório de Produtos Naturais, Instituto de Química, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Cecília Maria Alves
authors.initials: CM
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Biodados, Biologia Celular e Desenvolvimento, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Gabriel da Rocha
authors.initials: Gda R
authors.lastname: Fernandes
authors.affiliation: Unidad de Biología Celular y Molecular, Corporación para Investigaciones Biológicas (CIB) and Escuela de Microbiología Universidad de Antioquia, Medellín, Colombia.
authors.forename: Orville
authors.initials: O
authors.lastname: Hernández
authors.affiliation: Unidad de Biología Celular y Molecular, Corporación para Investigaciones Biológicas (CIB) and Facultad de Medicina Universidad de Antioquia, Medellín, Colombia.
authors.forename: Juan Guillermo McEwen
authors.initials: JG
authors.lastname: Ochoa
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Célia Maria de Almeida
authors.initials: CM
authors.lastname: Soares
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Maristela
authors.initials: M
authors.lastname: Pereira
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects*; D020869:Gene Expression Profiling; D031331:Hyptis / Q000737:chemistry; D007783:Lactones / Q000302:isolation & purification / Q000378:metabolism*; D018384:Oxidative Stress; D010228:Paracoccidioides / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000935:Antifungal Agents; D007783:Lactones; C108954:argentilactone