title: Evaluation of Microdissection Testicular Sperm Extraction Results in Patients with Non-Obstructive Azoospermia: Independent Predictive Factors and Best Cutoff Values for Sperm Retrieval.
abstract: OBJECTIVE
  Testicular sperm extraction (TESE) for intracytoplasmic sperm injection (ICSI) was first introduced for the treatment of non-obstructive azoospermia. This study was conducted to detect predictive factors affecting the success of microTESE.
  
  
  METHODS
  We retrospectively evaluated the results of 191 cases who underwent microTESE. For each patient, the testicular volume, endocrine profile [follicle stimulating hormone (FSH), luteinizing hormone (LH), free testosterone (FT), total testosterone (TT)], serum inhibin B level, karyotype analysis, and Y chromosome microdeletions were recorded, and all data were analyzed to detect any predictors. The receiver operating characteristic curve, two-sample t-test and regression analysis were used for the statistical analysis.
  
  
  RESULTS
  The mean age of the patients was 34.4 ± 5.6 years. Sperm retrieval was successful in 104 (54.5%) patients, and there was no sperm in 87 (45.5%). Seven factors including, testicular size, Johnson score, Y chromosome microdeletion, and serum FSH, LH, FT and TT levels were different between the successful and unsuccessful groups. Six patients had Klinefelter syndrome, and ten patients (5.2%) had a Y chromosome microdeletion (5 AZF-c, 1 AZF-b, 2 AZF-bc, 1 AZF-abc, and 1 AZF-ac). The Johnson score, TT level, family history and Y chromosome microdeletions were determined to be independent predictive factors for sperm found. According to the testicular histology, the sperm-found ratios were 36%, 48.6%, and 95.5% in the sertoli cell only syndrome, maturation arrest, and hypospermatogenesis groups, respectively.
  
  
  CONCLUSIONS
  According to our results, the Johnson score, TT level, family history-related infertility, and Y chromosome microdeletions were determined to be independent predictive factors for sperm found.
authors.affiliation: Department of Urology, Medical Faculty of Mugla, Mugla Sitki Kocman University, Mugla, Turkey. drmemoly@yahoo.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Cetinkaya
authors.affiliation: Department of Urology, Medical Faculty of 19 Mayis University, 19 Mayis University, Samsun, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Onem
authors.affiliation: Department of Urology, Rize Medical Faculty, Rize University, Rize, Turkey.
authors.forename: Orhan Unal
authors.initials: OU
authors.lastname: Zorba
authors.affiliation: Department of Urology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Hamdi
authors.initials: H
authors.lastname: Ozkara
authors.affiliation: Department of Urology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Alici
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000097:blood / Q000209:etiology* / Q000601:surgery*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D004210:Dissection; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007248:Infertility, Male; D007265:Inhibins / Q000097:blood; D059785:Karyotype; D007713:Klinefelter Syndrome / Q000150:complications; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008866:Microsurgery; D009929:Organ Size; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000150:complications; D053625:Sperm Retrieval; D013737:Testis / Q000473:pathology; D013739:Testosterone / Q000097:blood; D016896:Treatment Outcome
chemical_list: C422364:inhibin B; D013739:Testosterone; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone