title: Expression of microRNA-452 via adenoviral vector inhibits non-small cell lung cancer cells proliferation and metastasis.
abstract: The microRNA miR-452 has been shown to function as a tumor suppressor. However, the cellular mechanism and potential application of miR-452-mediated cancer suppression remain great unknown. This study aims to identify how miR-452 acts in regulating non-small cell lung cancer (NSCLC) proliferation and metastasis. Expression of miR-452 via adenoviral (Ad) vector inhibits the proliferation, invasion, and migration of NSCLC cells A549 or H460. Our data also shows that miR-452 down-regulates the expression of Bmi-1 as well as pro-survival or anti-apoptosis regulators Survivin, cIAP-1, and cIAP-2. By such gene interference, miR-452 modulates NSCLC cell epithelial-mesenchymal transition (EMT) and further disrupts their migration and invasion. Moreover, miR-452 blocks the activation of PI3K/AKT pathway, which is also required for EMT process. These data reveal that miR-452 treatment could be a novel target or strategy for NSCLC treatment.
authors.affiliation: Department of Respiratory Diseases, The 463 Hospital of Chinese PLA, Shenyang, 110042, People's Republic of China. yszhang2001@126.com.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Unit II, Department of Medical Oncology, The General Hospital of Chinese PLA, Beijing, 100853, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Respiratory Diseases, The 463 Hospital of Chinese PLA, Shenyang, 110042, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Pang
authors.affiliation: Department of Respiratory Diseases, The 463 Hospital of Chinese PLA, Shenyang, 110042, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Command Area, Shenyang, 110016, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.affiliation: Center of Technical and Service, The 302nd Hospital of Chinese PLA, Beijing, 100039, People's Republic of China.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000556:secondary*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D014162:Transfection
chemical_list: C574051:MIRN452 microRNA, human; D035683:MicroRNAs
keywords: Bmi-1; EMT; Metastasis; NSCLC; PI3K/AKT signaling pathway; miR-452