title: Influence of transfusion of lymphokine-activated T killer cells on inflammatory responses in dogs after laparotomy.
abstract: The influence of transfusion of lymphokine-activated T killer cells (T-LAK) on inflammatory responses was examined in dogs after laparotomy. Plasma C-reactive protein (CRP) level, cell numbers of peripheral blood lymphocytes (PBLs) and T lymphocyte subsets (CD3(+), CD4(+) and CD8(+)) and mRNA expression levels of cytokines including interleukin (IL)-2, IL-12, IL-4, IL-10 and transforming growth factor (TGF)-β in peripheral blood mononuclear cells (PBMCs) were measured in dogs with (T-LAK group) or without (control group) a single T-LAK administration immediately after laparotomy. The plasma CRP level initially increased and then decreased to the normal range at 7 days after laparotomy in the T-LAK group, which was earlier than in the control group. The expression level of IL-10 mRNA showed a marked postoperative increase and was significantly higher than the preoperative level on day 7 (P<0.05), whereas the level in the control group showed no clear change after laparotomy. A significant increase in IL-2 mRNA expression level in the T-LAK group was observed on day 14, which was two weeks earlier than in the control group (P<0.05). These results suggest that T-LAK therapy in dogs after laparotomy leads to earlier resolution of postoperative inflammation by production of an anti-inflammatory cytokine (IL-10) in the early phase of the postoperative period and earlier restoration of cell-mediated immunity related to cytokine production by PBMCs.
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Nish 2-sen 11 Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Mie
authors.forename: Mizuki
authors.initials: M
authors.lastname: Tomihari
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hoshi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Kazuro
authors.initials: K
authors.lastname: Miyahara
authors.forename: Terumasa
authors.initials: T
authors.lastname: Shimada
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism; D004285:Dogs; D007249:Inflammation / Q000628:therapy / Q000821:virology*; D007378:Interleukins / Q000378:metabolism; D015979:Killer Cells, Lymphokine-Activated / Q000637:transplantation*; D007813:Laparotomy / Q000662:veterinary; D008297:Male; D055611:Natural Killer T-Cells / Q000637:transplantation*; D009504:Neutrophils / Q000276:immunology; D011183:Postoperative Complications / Q000628:therapy / Q000662:veterinary*
chemical_list: D007378:Interleukins; D002097:C-Reactive Protein