title: MicroRNA-214 targets PCBP2 to suppress the proliferation and growth of glioma cells.
abstract: PCBP2, a member of the poly(C)-binding protein (PCBP) family, is involved in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs. Recent studies have shown that PCBP2 is overexpressed and plays an important role in human cancers, including glioma. However, the molecular basis for its up-regulation remains poorly understood. Here, we show that microRNA-214 (miR-214) interacts with the 3'-untranslated region of PCBP2 mRNA and induces its degradation, leading to reductions in its protein expression. As a result, overexpression of miR-214 mimics significantly inhibited, while its antisense oligos proliferation and growth of glioma cells. Restoration of PCBP2 remarkably reversed the tumor-suppressive effects of miR-214 on cell proliferation and growth. In summary, our data indicate that miR-214 may function as tumor suppressor in glioma by targeting PCBP2.
authors.affiliation: Department of Neurosurgery, Huaihe Hospital, Henan University Kaifeng 475000, China.
authors.forename: Shi-Lei
authors.initials: SL
authors.lastname: Tang
authors.affiliation: Department of Neurology, Kaifeng Central Hospital Kaifeng 475000, China.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Huaihe Hospital, Henan University Kaifeng 475000, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Chen
mesh_terms: D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs; C501903:PCBP2 protein, human; D016601:RNA-Binding Proteins
keywords: PCBP2; glioma; miR-214; tumor suppressor