title: Correlation between matrix metalloproteinase-9 and endometriosis.
abstract: Endometrial implantation is the major cause of endometriosis (EMS). Matrix metalloproteinase (MMPs) can degrade multiple extracellular matrix and has been postulated to be related with EMC occurrence. This study thus investigated serum and ascites levels of MMP-9 in EMS patients, in an attempt to discuss the correlation between MMP-9 and EMS. A total of 100 EMS patients, including eutopic endometrium and ectopic endometrium, were recruited in this study along with hysteromyoma patients as the control group. Peripheral blood and ascites samples were collected and tested for MMP-9 levels using gelatin zymogram and enzyme-linked immunosorbent assay (ELISA). In EMS patients, MMP-9 levels in serum and ascites were 6.24 ± 0.53 mM and 38.57 ± 4.93 mM, respectively. Both of them were significantly higher than those in control group (P<0.05). Eutopic endometrium group had higher MMP-9 levels compared to those in ectopic endometrium ones (P<0.05). With advancement of disease stage, EMS patients had progressively elevated MMP-9 levels (P<0.05). Patients at proliferative stage had higher MMP-9 secretion (P<0.05). In summary, site of endometrium, clinical stage and proliferative cycle were independent risk factors for EMS. The elevation of serum and ascites MMP-9 existed in EMS patients, of which those had ectopic endometrium, advanced stage and at proliferative stage had higher MMP-9 expression.
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Tang
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Reproduction, Reproductive Medicine Center, Jinan Military General Hospital Jinan 250031, Shandong, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Hao
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000737:chemistry / Q000378:metabolism; D004715:Endometriosis / Q000201:enzymology* / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D055815:Young Adult
chemical_list: C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Endometriosis; ascites; matrix metalloproteinase; serum