title: MicroRNA-127-3p inhibits proliferation and invasion by targeting SETD8 in human osteosarcoma cells.
abstract: MicroRNAs (miRNAs) play an essential role in cancer development. Several studies have indicated that miRNAs mediate tumorigenesis processes, such as, inflammation, proliferation, apoptosis and invasion. In the present study, we focused on the influence of the miR-127-3p on the proliferation, migration and invasion of osteosarcoma (OS). MiR-127-3p was found at reduced levels in OS tissues and cell lines. Overexpression of miR-127-3p in the OS cell lines significantly inhibited the cell proliferation, migration and invasion; however, inhibition of miR-127-3p increased the proliferation, migration and invasion of OS in vitro. SETD8 was identified as a direct target of miR-127-3p, and SETD8 expression decreased post miR-127-3p overexpression, while SETD8 overexpression could reverse the potential influence of miR-127-3p on the migration and invasion of OS cells. MiR-127-3p is suggested to act mainly via the suppression of SETD8 expression. Overall, the results revealed that miR-127-3p acts as a tumor suppressor and that its down-regulation in cancer may contribute to OS progression and metastasis, suggesting that miR-127-3p could be a potential therapeutic target in the treatment of OS.
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Wengen
authors.initials: W
authors.lastname: Hou
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Chai
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Jia
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Sun
authors.affiliation: Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital, ZhengZhou University, ZhengZhou 450000, PR China. Electronic address: wangranfire@sohu.com.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D011485:Protein Binding
chemical_list: C547276:MIRN127 microRNA, human; D035683:MicroRNAs; D011495:Histone-Lysine N-Methyltransferase; C470730:KMT5A protein, human
keywords: Invasion; Migration; OS; Proliferation; SETD8; miR-127-3p