title: Penicillium oxalicum PoFlbC regulates fungal asexual development and is important for cellulase gene expression.
abstract: Filamentous fungi can initiate vegetative growth on complex plant polysaccharides in nature through secreting a large amount of lignocellulose-degrading enzymes. These fungi develop a large amount of asexual spores to disperse and survive under harsh conditions, such as carbon and nitrogen depletion. Numerous studies report the presence of a cross-talk between asexual development and extracellular enzyme production, especially at the regulation level. This study identified and characterized a C2H2-type transcription factor called PoFlbC, which is an Aspergillus FlbC ortholog, in cellulolytic fungus Penicillium oxalicum. Results showed that the native level of PoFlbC was crucial for the normal growth and asexual development of P. oxalicum. Importantly, deletion of the PoflbC gene substantially reduced cellulase and hemicellulase productions. Comparative transcriptome analysis by RNA sequencing revealed a global downregulation of genes encoding cellulases, hemicellulases, and other proteins with functions in lignocellulose degradation. A similar defect was also observed in the OEPoflbC strain, suggesting that the production of cellulolytic enzymes was maintained by native expression of the PoflbC. In this study, an essential activator for both fungal asexual development and cellulase production was established in P. oxalicum.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China.
authors.forename: Guangshan
authors.initials: G
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China; Qingdao Vland Biotech Group Co. Ltd., Shandong Expressway Mansion, Miaoling Road, Qingdao, Shandong, PR China. Electronic address: lzhlzh@vip.126.com.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China.
authors.forename: Ruimei
authors.initials: R
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China; National Glycoengineering Research Center, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China; National Glycoengineering Research Center, Shandong University, Shan Da Nan Road 27, Jinan, Shandong 250100, PR China. Electronic address: quyinbo@sdu.edu.cn.
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
mesh_terms: D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors; D002480:Cellulase
keywords: Cellulase; Conidiation; Hemicellulase; Penicillium oxalicum; PoFlbC; Transcription factor