title: MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus.
abstract: Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.|Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Hezova
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Kovarikova
authors.affiliation: Olomouc, Faculty of Medicine, Palacky University, Olomouc, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Srovnal
authors.affiliation: Oncology Department, General University Hospital in Prague, Praha, Czech Republic.
authors.forename: Milada
authors.initials: M
authors.lastname: Zemanova
authors.affiliation: Department of Surgery, University Hospital in Motol, Praha, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Harustiak
authors.affiliation: Olomouc, Faculty of Medicine, Palacky University, Olomouc, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Ehrmann
authors.affiliation: Olomouc, Faculty of Medicine, Palacky University, Olomouc, Czech Republic.
authors.forename: Marian
authors.initials: M
authors.lastname: Hajduch
authors.affiliation: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
authors.forename: Milana
authors.initials: M
authors.lastname: Sachlova
authors.affiliation: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Svoboda
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic. on.slaby@gmail.com.|Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic. on.slaby@gmail.com.|Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, Brno, 625 00, Czech Republic. on.slaby@gmail.com.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Slaby
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D009857:Oncogenes / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D035683:MicroRNAs; C534425:MIR205, human
keywords: Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; Oncogene; Tumor suppressor; miR-205