title: [Immunomodulation of Uncaria tomentosa over dendritic cells, il-12 and profile TH1/TH2/TH17 in breast cancer].
abstract: Objetives. This study aimed to research the in vitro immunomodulatory effects of an Uncaria tomentosa hydroalcoholic extract standardized (5.03%, pentacyclic oxindole alkaloids) (UT-POA) on the immunophenotype of dendritic cells (DC) subsets, Th1, Th2, Th17 and IL-12 cytokines from patients with stage II breast cancer (BCII) and healthy women (H).
  
  
  METHODS
  Blood of 11 H and 7 BCII was obtained, PBMC were isolated and cultured for 2h with/without various concentrations of UT-POA and stimulated or not with LPS for 24h. PBMC were labeled with specific antibodies for DC and in the supernatant we measured Th1/Th2/Th17 cytokines, both by flow cytometry. Furthermore IL-12 was measured by ELISA.
  
  
  RESULTS
  UT-POA did not alter DC or accessory molecules expression in BCII. However, H exhibited a decrease in the percentage of mDC (myeloid DC) and an increase in HLA-DR and CD86 expression at 1000 μg/mL. IL-12 secretion was modified only in the H group, increasing p70 subunit and decreasing p40 subunit. UT-POA increased Th1 (IFN-γ and IL-2), Th2 (IL-4) and Th17 (IL-17) secretion in both groups.
  
  
  CONCLUSIONS
  UT-POA increased the production of cytokines related with anti-tumoral response at concentrations of 500-1000 μg/mL. This positive effect should be evaluated not only systemically but also in the tumor microenvironment in further studies. UT-POA may be a useful phytochemical in chemoprevention and in the alternative use in cancer therapies.
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: César
authors.initials: C
authors.lastname: Núñez
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Iván
authors.initials: I
authors.lastname: Lozada-Requena
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Tíndara
authors.initials: T
authors.lastname: Ysmodes
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Daniel
authors.initials: D
authors.lastname: Zegarra
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Fatima
authors.initials: F
authors.lastname: Saldaña
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: José
authors.initials: J
authors.lastname: Aguilar
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000276:immunology; D020948:Cat's Claw; D003713:Dendritic Cells; D005260:Female; D006801:Humans; D056747:Immunomodulation; D018664:Interleukin-12; D007963:Leukocytes, Mononuclear; D010936:Plant Extracts / Q000627:therapeutic use*
chemical_list: D010936:Plant Extracts; D018664:Interleukin-12