title: Simultaneous glucose and xylose uptake by an acetone/butanol/ethanol producing laboratory Clostridium beijerinckii strain SE-2.
abstract: OBJECTIVE
  Most butanol-producing strains of Clostridium prefer glucose over xylose, leading to a slower butanol production from lignocellulose hydrolysates. It is therefore beneficial to find and use a strain that can simultaneously use both glucose and xylose.
  
  
  RESULTS
  Clostridium beijerinckii SE-2 strain assimilated glucose and xylose simultaneously and produced ABE (acetone/butanol/ethanol). The classic diauxic growth behavior was not seen. Similar rates of sugar consumption (4.44 mM glucose h(-1) and 6.66 mM xylose h(-1)) were observed suggesting this strain could use either glucose or xylose as the substrate and it has a similar capability to degrade these two sugars. With different initial glucose:xylose ratios, glucose and xylose were consumed simultaneously at rates roughly proportional to their individual concentrations in the medium, leading to complete utilization of both sugars at the same time.
  
  
  CONCLUSIONS
  ABE production profiles were similar on different substrates. Transcriptional studies on the effect of glucose and xylose supplementation, however, suggests a clear glucose inhibition on xylose metabolism-related genes is still present.
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Jinan, China.|Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan, China.|Shanghai Key Laboratory of Bio-Energy Crops, Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shandong Science and Technology Statistical Analysis and Research Center, Jinan, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Jinan, China.|Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan, China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Xu
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Jinan, China.|Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Jinan, China.|Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Hua
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Jinan, China.|Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Jin
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, Shanghai, China.
authors.forename: Mintian
authors.initials: M
authors.lastname: Gao
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Jinan, China. zhangxd@sderi.cn.|Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan, China. zhangxd@sderi.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000096:Acetone / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000254:growth & development* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014994:Xylose / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D000440:Butanols; D000096:Acetone; D000431:Ethanol; D014994:Xylose; D005947:Glucose
keywords: Acetone/butanol/ethanol; Butanol; Clostridium beijerinckii SE-2; Glucose; Simultaneous uptake; Xylose