title: Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology.
abstract: Whey is an abundant by-product of cheese production process and it is considered a special waste due to its high nutritional load and hypertrophic potential. Technologies for whey valorization are available. They can convert such waste into high-value products, like whey proteins. However, the remaining liquid (called permeate) is still considered as a polluting waste due to its high lactose concentration. The alcoholic fermentation of lactose into ethanol will simultaneously achieve two important goals: safe disposal of a pollutant waste and green energy production. This methodology paper illustrates the workflow carried out to design and realize an optimized microorganism that can efficiently perform the lactose-to-ethanol conversion, engineered via synthetic biology experimental and computational approaches.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Pasotti
authors.forename: Susanna
authors.initials: S
authors.lastname: Zucca
authors.forename: Michela
authors.initials: M
authors.lastname: Casanova
authors.forename: Nicolo
authors.initials: N
authors.lastname: Politi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Massaiu
authors.forename: Giuliano
authors.initials: G
authors.lastname: Mazzini
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Micoli
authors.forename: Cinzia
authors.initials: C
authors.lastname: Calvio
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Cusella De Angelis
authors.forename: Paolo
authors.initials: P
authors.lastname: Magni
mesh_terms: D055162:Biocatalysis; D056804:Biofuels; D002611:Cheese; D000431:Ethanol; D007785:Lactose; D058615:Synthetic Biology
chemical_list: D056804:Biofuels; D000431:Ethanol; D007785:Lactose