title: Preparation of Rat Whole-kidney Acellular Matrix via Peristaltic Pump.
abstract: To design a whole-kidney a cellular matrix scaffold using peristaltic pump perfusion and to ascertain the retention of extra cellular proteins by the scaffold.
  
  
  
  Male Sprague-Dawley (SD) rats weighing 200-250 g were used. Intravenous catheters were inserted into the renal artery followed by perfusion of decellularization solution using a peristaltic pump. After decellularization, the acellular matrix was observed under a microscope after hematoxylin and eosin (H&E) staining and a fluorescence microscope after 4′,6-diamidino-2-phenylindole (DAPI) staining. Immunohistochemistry was used to identify the composition of kidney acellular matrix.
  
  
  
  The result of H&E and DAPI staining demonstrate the removal of cellular material in kidney a cellular matrix. Immunohistochemistry confirmed the conservation of the natural expression of extra cellular matrix proteins including collagen types I and IV, fibrin and laminin.
  
  
  
  Peristaltic pump perfusion enables successful preparation of renal a cellular matrix, to retainthe criticalproteins of natural extra cellular matrix. The resulting kidney a cellular matrix represents an ideal natural scaffold for renal tissue engineering.
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China. lliurf@126.com.
authors.forename: Rong Fu
authors.initials: RF
authors.lastname: Liu
authors.affiliation: Department of Urology, the First People Hospital of Jiujiang City, Jiujiang 332000, China.
authors.forename: Jia sheng
authors.initials: JS
authors.lastname: Gao
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
authors.forename: Yu Feng
authors.initials: YF
authors.lastname: Yang
authors.affiliation: Department of Urology, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
authors.forename: Wei Xin
authors.initials: WX
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002469:Cell Separation / Q000379:methods*; D024042:Collagen Type I / Q000032:analysis; D024141:Collagen Type IV / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry*; D005337:Fibrin / Q000032:analysis; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry / Q000166:cytology*; D007797:Laminin / Q000032:analysis; D008297:Male; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D024141:Collagen Type IV; D007797:Laminin; D005337:Fibrin