title: Lactic Acid Bacteria Isolated from Bovine Mammary Microbiota: Potential Allies against Bovine Mastitis.
abstract: Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.
authors.forename: Damien S
authors.initials: DS
authors.lastname: Bouchard
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.|Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Bianca
authors.initials: B
authors.lastname: Seridan
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.
authors.forename: Taous
authors.initials: T
authors.lastname: Saraoui
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Rault
authors.affiliation: UMR 1282 Infectiologie et Santé Publique, INRA, Nouzilly, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Germon
authors.affiliation: CERELA-CONICET, Centro de Referencia por los Lactobacillus, San Miguel de Tucumán, Argentina.
authors.forename: Candelaria
authors.initials: C
authors.lastname: Gonzalez-Moreno
authors.affiliation: CERELA-CONICET, Centro de Referencia por los Lactobacillus, San Miguel de Tucumán, Argentina.
authors.forename: Fatima M E
authors.initials: FM
authors.lastname: Nader-Macias
authors.affiliation: Genomic Research Laboratory, Geneva University Hospital, Geneva, Switzerland.
authors.forename: Damien
authors.initials: D
authors.lastname: Baud
authors.affiliation: Genomic Research Laboratory, Geneva University Hospital, Geneva, Switzerland.
authors.forename: Patrice
authors.initials: P
authors.lastname: François
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.
authors.forename: Victoria
authors.initials: V
authors.lastname: Chuat
authors.affiliation: INRA-MICALIS, Jouy-En-Josas, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Chain
authors.affiliation: INRA-MICALIS, Jouy-En-Josas, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Jacques
authors.initials: J
authors.lastname: Nicoli
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.affiliation: UMR 1253 STLO, INRA, Rennes, France.|UMR1253 STLO, Agrocampus Ouest, Rennes, France.
authors.forename: Sergine
authors.initials: S
authors.lastname: Even
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D005260:Female; D016680:Genome, Bacterial; D019073:HT29 Cells; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification*; D017045:Lactococcus / Q000302:isolation & purification*; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D064307:Microbiota; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S