title: Construction of Radial Defect Models in Rabbits to Determine the Critical Size Defects.
abstract: Many studies aimed at investigating bone repair have been conducted through animal models in recent years. However, limitations do exist in these models due to varying regeneration potential among different animal species. Even using the same animal, big differences exist in the size of critical size defects (CSD) involving the same region. This study aimed to investigate the standardization of radial bone defect models in rabbits and further establish more reliable CSD data. A total of 40 6-month-old New Zealand white rabbits of clean grade totaling 80 radial bones were prepared for bone defect models, according to the principle of randomization. Five different sizes (1.0, 1.2, 1.4, 1.7 and 2.0 cm) of complete periosteal defects were introduced under anesthesia. At 12 weeks postoperatively, with the gradual increase in defect size, the grades of bone growth were significantly decreased in all 5 groups. X-ray, CT scans and H&E staining of the 1.4, 1.7, and 2.0-cm groups showed lower grades of bone growth than that of the 1.0 and 1.2-cm groups respectively (P < 0.05). Using rabbit radial defect model involving 6-month-old healthy New Zealand white rabbits, this study indicates that in order to be critical sized, defects must be greater than 1.4 cm.
authors.affiliation: Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Ming-Dong
authors.initials: MD
authors.lastname: Zhao
authors.affiliation: Department of Occupational medicine, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Jian-Shu
authors.initials: JS
authors.lastname: Huang
authors.affiliation: Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Xin-Chao
authors.initials: XC
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Gui
authors.affiliation: Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiong
authors.affiliation: Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Wang-Ping
authors.initials: WP
authors.lastname: Yin
authors.affiliation: Department of Orthopaedics and Central Laboratory, the third Hospital Affiliated to Nantong University, Wuxi, Jiangsu, 214041, China.
authors.forename: Feng-Lai
authors.initials: FL
authors.lastname: Yuan
authors.affiliation: Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Cai
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D023421:Models, Animal; D011817:Rabbits; D011884:Radius / Q000254:growth & development*