title: Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico.
abstract: Vibrio mimicus is a gram-negative bacterium responsible for diseases in humans. Three strains of V. mimicus identified as V. mimicus 87, V. mimicus 92 and V. mimicus 93 were isolated from a shrimp processing facility in Guaymas, Sonora, Mexico. The strains were analyzed using several molecular techniques and according to the cluster analysis they were different, their similarities ranged between 51.3% and 71.6%. ERIC-PCR and RAPD (vmh390R) were the most discriminatory molecular techniques for the differentiation of these strains. The complete genomes of two strains (V. mimicus 87, renamed as CAIM 1882, and V. mimicus 92, renamed as CAIM 1883) were sequenced. The sizes of the genomes were 3.9 Mb in both strains, with 2.8 Mb in ChI and 1.1 Mb in ChII. A 12.7% difference was found in the proteome content (BLAST matrix). Several virulence genes were detected (e.g. capsular polysaccharide, an accessory colonization factor and genes involved in quorum-sensing) which were classified in 16 categories. Variations in the gene content between these genomes were observed, mainly in proteins and virulence genes (e.g., hemagglutinin, mobile elements and membrane proteins). According to these results, both strains were different, even when they came from the same source, giving an insight of the diversity of V. mimicus. The identification of various virulence genes, including a not previously reported V. mimicus gene (acfD) in ChI in all sequenced strains, supports the pathogenic potential of this species. Further analysis will help to fully understand their potential virulence, environmental impact and evolution.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C Hermosillo, Sonora, México.
authors.forename: Iliana
authors.initials: I
authors.lastname: Guardiola-Avila
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C Hermosillo, Sonora, México.
authors.forename: Evelia
authors.initials: E
authors.lastname: Acedo-Felix
authors.affiliation: Mazatlán Unit for Aquaculture and Environmental Management. Centro de Investigación en Alimentación y Desarrollo, A.C. Mazatlán, Sinaloa, México.
authors.forename: Itzel
authors.initials: I
authors.lastname: Sifuentes-Romero
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C Hermosillo, Sonora, México.
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
authors.affiliation: Mazatlán Unit for Aquaculture and Environmental Management. Centro de Investigación en Alimentación y Desarrollo, A.C. Mazatlán, Sinaloa, México.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gomez-Gil
authors.affiliation: Guaymas Unit: Quality Assurance and Management of Natural Resources. Centro de Investigación en Alimentación y Desarrollo, A.C. Guaymas, Sonora, México.
authors.forename: Lorena
authors.initials: L
authors.lastname: Noriega-Orozco
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005506:Food Contamination; D005511:Food Handling; D005516:Food Microbiology; D005615:Freezing; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D008800:Mexico; D033561:Penaeidae / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D044164:Vibrio mimicus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio