title: Arabidopsis TAF15b Localizes to RNA Processing Bodies and Contributes to snc1-Mediated Autoimmunity.
abstract: In both animals and plants, messenger (m)RNA export has been shown to contribute to immune response regulation. The Arabidopsis nuclear protein MOS11, along with the nucleoporins MOS3/Nup96/SAR3 and Nup160/SAR1 are components of the mRNA export machinery and contribute to immunity mediated by nucleotide binding leucine-rich repeat immune receptors (NLR). The human MOS11 ortholog CIP29 is part of a small protein complex with three additional members: the RNA helicase DDX39, ALY, and TAF15b. We systematically assessed the biological roles of the Arabidopsis homologs of these proteins in toll interleukin 1 receptor-type NLR (TNL)-mediated immunity using reverse genetics. Although mutations in ALY and DDX39 did not result in obvious defects, taf15b mutation partially suppressed the autoimmune phenotypes of a gain-of-function TNL mutant, snc1. An additive effect on snc1 suppression was observed in mos11-1 taf15b snc1 triple mutant plants, suggesting that MOS11 and TAF15b have independent functions. TAF15b-GFP fusion protein, which fully complemented taf15b mutant phenotypes, localized to nuclei similarly to MOS11. However, it was also targeted to cytosolic granules identified as processing bodies. In addition, we observed no change in SNC1 mRNA levels, whereas less SNC1 protein accumulated in taf15b mutant, suggesting that TAF15b contributes to SNC1 homeostasis through posttranscriptional mechanisms. In summary, this study highlights the importance of posttranscriptional RNA processing mediated by TAF15b in the regulation of TNL-mediated immunity.
authors.affiliation: 1 Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada;|2 Michael Smith Laboratories, University of British Columbia;
authors.forename: Oliver X
authors.initials: OX
authors.lastname: Dong
authors.affiliation: 3 Department of Biology, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada;
authors.forename: Louis-Valentin
authors.initials: LV
authors.lastname: Meteignier
authors.affiliation: 4 Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, QC, G9A 5H7, Canada;|5 Groupe de Recherche en Biologie Végétale (GRBV), Université du Québec à Trois-Rivières; and.
authors.forename: Melodie B
authors.initials: MB
authors.lastname: Plourde
authors.affiliation: 4 Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, QC, G9A 5H7, Canada;|5 Groupe de Recherche en Biologie Végétale (GRBV), Université du Québec à Trois-Rivières; and.
authors.forename: Bulbul
authors.initials: B
authors.lastname: Ahmed
authors.affiliation: 6 Department of Plant Pathology and Microbiology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, U.S.A.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: 2 Michael Smith Laboratories, University of British Columbia;
authors.forename: Cassandra
authors.initials: C
authors.lastname: Jensen
authors.affiliation: 6 Department of Plant Pathology and Microbiology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, U.S.A.
authors.forename: Hailing
authors.initials: H
authors.lastname: Jin
authors.affiliation: 3 Department of Biology, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada;
authors.forename: Peter
authors.initials: P
authors.lastname: Moffett
authors.affiliation: 1 Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada;|2 Michael Smith Laboratories, University of British Columbia;
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: 4 Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, QC, G9A 5H7, Canada;|5 Groupe de Recherche en Biologie Végétale (GRBV), Université du Québec à Trois-Rivières; and.
authors.forename: Hugo
authors.initials: H
authors.lastname: Germain
mesh_terms: D021581:Active Transport, Cell Nucleus; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000276:immunology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D046912:Multiprotein Complexes; D009154:Mutation; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D030821:Plants, Genetically Modified; D012323:RNA Processing, Post-Transcriptional / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000276:immunology
chemical_list: D029681:Arabidopsis Proteins; C556905:MOS11 protein, Arabidopsis; C507155:MOS3 protein, Arabidopsis; D046912:Multiprotein Complexes; D028861:Nuclear Pore Complex Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C482739:SNC1 protein, Arabidopsis