title: Identification, characterization and description of Arcobacter faecis sp. nov., isolated from a human waste septic tank.
abstract: A study on the taxonomic classification of Arcobacter species was performed on the cultures isolated from various fecal sources where an Arcobacter strain AF1078(T) from human waste septic tank near Ottawa, Ontario, Canada was characterized using a polyphasic approach. Genetic investigations including 16S rRNA, atpA, cpn60, gyrA, gyrB and rpoB gene sequences of strain AF1078(T) are unique in comparison with other arcobacters. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain is most closely related to Arcobacter lanthieri and Arcobacter cibarius. Analyses of atpA, cpn60, gyrA, gyrB and rpoB gene sequences suggested that strain AF1078(T) formed a phylogenetic lineage independent of other species in the genus. Whole-genome sequence, DNA-DNA hybridization, fatty acid profile and phenotypic analysis further supported the conclusion that strain AF1078(T) represents a novel Arcobacter species, for which the name Arcobacter faecis sp. nov. is proposed, with type strain AF1078(T) (=LMG 28519(T); CCUG 66484(T)).
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: Kerri
authors.initials: K
authors.lastname: Whiteduck-Léveillée
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: Jenni
authors.initials: J
authors.lastname: Whiteduck-Léveillée
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Cloutier
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: James T
authors.initials: JT
authors.lastname: Tambong
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: Renlin
authors.initials: R
authors.lastname: Xu
authors.affiliation: London Research and Development Centre (LRDC), Agriculture and Agri-Food Canada, London, ON, Canada.
authors.forename: Edward
authors.initials: E
authors.lastname: Topp
authors.affiliation: Ryerson University, Department of Chemistry and Biology, Toronto, ON, Canada.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Arts
authors.affiliation: Ontario Ministry of the Environment, Toronto, ON, Canada.
authors.forename: Jerry
authors.initials: J
authors.lastname: Chao
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: Zaky
authors.initials: Z
authors.lastname: Adam
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: C André
authors.initials: CA
authors.lastname: Lévesque
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: David R
authors.initials: DR
authors.lastname: Lapen
authors.affiliation: INRS-Institute Armand-Frappier Research Centre, Laval, QC, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Villemur
authors.affiliation: Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada. Electronic address: izhar.khan@agr.gc.ca.
authors.forename: Izhar U H
authors.initials: IU
authors.lastname: Khan
mesh_terms: D020640:Arcobacter / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure; D015373:Bacterial Typing Techniques; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D017422:Sequence Analysis, DNA
chemical_list: D005227:Fatty Acids
keywords: Arcobacter faecis; Genus Arcobacter; Human septic tank; Novel species