title: Effect of losartan with folic acid on plasma homocysteine and vascular ultrastructural changes in spontaneously hypertensive rats.
abstract: Elevated homocysteine (Hcy) is a high risk factor of hypertension due to its function in endothelial dysfunction. Its level in the blood is strongly influenced by folic acid. In order to investigate the effects of losartan with folic acid on plasma level of Hcy and vascular ultrastructural changes, thirty spontaneously hypertensive rats (SHR) involved and randomly divided into three groups (n=10): SHR-C group (control), SHR-L group (losartan 25 mg · kg(-1) · d(-1)), SHR-L+Y group (losartan 25 mg · kg(-1) · d(-1) + folic acid 0.4 mg · kg(-1) · d(-1)). Another 10 Wistar Rats involved as WKY-C group for normal control. The level of plasma Hcy was measured dynamically by LS-MS, the vascular ultrastructural changes were analyzed by light and electron microscopy. Moreover, the thickness and area of aorta was measured. The results showed the Hcy levels in four groups were WKY-C 7.49 ± 1.95 μmol/L; SHR-C 8.45 ± 1.90 μmol/L; SHR-L 8.28 ± 2.11 μmol/L; SHR-L+Y 7.53 ± 2.02 μmol/L at 80 days. There was no significant change for plasma Hcy (P>0.05). The morphological change showed the subendothelial space didn't increased significantly, the endothelial cells have a more smooth and intact cellular membrane in SHR-L+Y group. In conclusion, Losartan combined with folic acid could improve arterial endothelial structure in SHR which has no significant correlation with plasma Hcy.
authors.affiliation: Department of Pathology, Integrated Chinese and Western Medicine Hospital of Wenzhou Wenzhou, China.
authors.forename: Lihe
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou, Zhejiang 310003, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Basic Medical Science, Inner Mongolia Medical University Hohhot, Inner Mongolia Jinshan Development Zone, China.
authors.forename: Baofu
authors.initials: B
authors.lastname: Jia
authors.affiliation: Department of Pathology, Integrated Chinese and Western Medicine Hospital of Wenzhou Wenzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, China.
authors.forename: Feiyan
authors.initials: F
authors.lastname: Lin
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000648:ultrastructure; D005492:Folic Acid / Q000494:pharmacology*; D006397:Hematinics / Q000494:pharmacology*; D006710:Homocysteine / Q000097:blood*; D006973:Hypertension / Q000503:physiopathology; D019808:Losartan / Q000494:pharmacology*; D008297:Male; D046529:Microscopy, Electron, Transmission; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D000959:Antihypertensive Agents; D006397:Hematinics; D006710:Homocysteine; D005492:Folic Acid; D019808:Losartan
keywords: Losartan; SHR; endothelium; folic acid; homocysteine