title: MYTH Screening: iMYTH and tMYTH Variants.
abstract: Once a bait has been generated and validated for the membrane yeast two-hybrid (MYTH) assay, it can be used for either high-throughput screening to generate a detailed interaction map (interactome) or in low-throughput experiments to examine interactions with specific targets. Here we describe how to carry out high-throughput MYTH library screening of a validated bait generated using integrated or traditional MYTH. The principles herein can be easily adapted for use in a smaller-scale format if required. A typical MYTH library screen can be completed in ∼3-4 wk.
authors.affiliation: Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada;
authors.forename: Jamie
authors.initials: J
authors.lastname: Snider
authors.affiliation: Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada; Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
authors.forename: Igor
authors.initials: I
authors.lastname: Stagljar
mesh_terms: D000818:Animals; D015723:Gene Library; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012474:Salmon; D020798:Two-Hybrid System Techniques
chemical_list: D008565:Membrane Proteins