title: Imbalanced expression of Bcl-xL and Bax in platelets treated with plasma from immune thrombocytopenia.
abstract: Immune thrombocytopenia is a heterogeneous autoimmune disease, characterized by accelerated platelet destruction and impaired platelet production. Bcl-xL and Bax play an opposite role in the regulation of apoptotic process with Bcl-xL for cell survival and Bax for cell apoptosis. Given the critical roles in the regulation of platelet apoptosis, whether Bcl-xL or Bax was involved in the pathogenesis of ITP remains unknown. The aim of this study is to evaluate the expression profile of Bcl-xL and Bax in platelets treated with ITP plasma. Normal washed platelets were treated with plasma from 20 active ITP patients or 10 age and gender-matched control to mimic the ITP in vivo environment. Mitochondrial depolarization, platelet apoptosis and activation were measured by flow cytometry. Expression of Bcl-xL, Bax and caspase-3 were also measured by quantitative real-time PCR and western blot. Our results demonstrated increased mitochondrial depolarization, platelet apoptosis and activation in platelets after treated with ITP plasma in comparison to control. In addition, decreased expression of Bcl-xL, increased expression of Bax and activity of caspase-3 were also observed. Furthermore, a negative correlation of Bcl-xL with Bax was found in platelets treated with ITP plasma. In conclusion, imbalanced expression of Bcl-xL and Bax might be associated with platelet apoptosis in ITP and therapeutically targeting them might be a novel approach in the treatment of ITP.
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Department of Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China.
authors.forename: Depeng
authors.initials: D
authors.lastname: Li
authors.affiliation: Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors.forename: Yulu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors.forename: Mingshan
authors.initials: M
authors.lastname: Niu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Hongchun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China. lihmd@163.com.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China. lihmd@163.com.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China. lihmd@163.com.
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China. zengly2000@163.com.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China. zengly2000@163.com.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China. zengly2000@163.com.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zeng
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000235:genetics; D001792:Blood Platelets / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics* / Q000276:immunology*; D055815:Young Adult; D051028:bcl-2-Associated X Protein / Q000235:genetics*; D051020:bcl-X Protein / Q000235:genetics*
chemical_list: D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D053148:Caspase 3
keywords: Apoptosis; Bax; Bcl-xL; Caspase-3; Immune thrombocytopenia