title: Tumor Associated Mesenchymal Stromal Cells Show Higher Immunosuppressive and Angiogenic Properties Compared to Adipose Derived MSCs.
abstract: BACKGROUND
  Differentiation, migratory properties and availability of Mesenchymal Stromal Cells (MSC) have become an important part of biomedical research. However, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells.
  
  
  OBJECTIVE
  To characterize and compare the phenotype and cytokines of adipose derived MSCs (AD-MSCs) and tumoral-MSCs (T-MSCs) isolated from mammary tumors of BALB/c mice.
  
  
  METHODS
  Immunophenotyping and in vitro differentiation tests were used for MSC characterization. Cytokine and enzyme profiles were assessed using ELISA and Real-time PCR, respectively.
  
  
  RESULTS
  T-MSCs expressed significantly higher levels of HLA-DR (p=0.04). Higher levels of PGE2 and COX-2 enzyme were also observed in T-MSCs (p=0.07 and p=0.00, respectively). Additionally, T-MSCs expressed higher levels of iNOS and MMP9 (p=0.01 and p=0.01, respectively). T-MSCs were also able to induce higher levels of proliferation and migration of HUVEC endothelial cells in wound scratch assay compared to AD-MSCs (p=0.015).
  
  
  CONCLUSIONS
  Functional differences showed by the surface markers of MSCs, cytokine and enzyme production indicate the effect of different microenvironments on MSCs phenotype and function.
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, e-mail: l.langroudi@modares.ac.ir.
authors.forename: Ladan
authors.initials: L
authors.lastname: Langroudi
authors.forename: Zuhair Muhammad
authors.initials: ZM
authors.lastname: Hassan
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Hashemi
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D060833:Cellular Microenvironment; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D007108:Immune Tolerance; D016130:Immunophenotyping; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D014945:Wound Healing
chemical_list: D016207:Cytokines