title: Effect of human recombinant granulocyte colony-stimulating factor on rat busulfan-induced testis injury.
abstract: Granulocyte colony-stimulating factor (G-CSF) is known for proliferation and anti-apoptotic activities. We aimed to use this growth factor in busulfan-injured testis. 32 male Wistar rats were injected with a double dosage of 15 ml/kg busulfan with 14 days interval. Administration of human recombinant G-CSF (100 µg/kg) subcutaneously was performed in two different time periods: 3 days before and 2 days after receiving busulfan, G-CSF1; and at days 14-18 of busulfan injection, G-CSF2. Animals were sacrificed at the end of week five. Histological analysis, testis weight and sperm parameters (sperm count and viability) has been checked. Expressions of DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), deleted in azoospermia like (DAZL), transition protein 2 (TP2), proliferating cell nuclear antigen (PCNA) and 5-Bromo-20-deoxyuridine (BrdU) were assessed. Empty seminiferous tubules were apparent in the busulfan- and G-CSF2-injected rats, but not in the G-CSF1 group. The G-CSF1-treated animals showed an increase in testis weight and sperm count and viability along with high expressions of DDX4, DAZL, TP2, PCNA and BrdU; even so, the changes were reversed in the busulfan and G-CSF2 groups (for all p < 0.05). Our results revealed that G-CSF application prior to busulfan insult is a promising approach in fertility maintenance.
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. Khanlarkhanineda@gmail.com.
authors.forename: Neda
authors.initials: N
authors.lastname: Khanlarkhani
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. pasbakhsh@hotmail.com.
authors.forename: Parichehr
authors.initials: P
authors.lastname: Pasbakhsh
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. keywan987@yahoo.com.
authors.forename: Keywan
authors.initials: K
authors.lastname: Mortezaee
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. naji_m_f@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Naji
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. amidifardin@yahoo.com.
authors.forename: Fardin
authors.initials: F
authors.lastname: Amidi
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. najafiat@gmail.com.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Najafi
authors.affiliation: Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran. sobhania@tums.ac.ir.
authors.forename: Aligholi
authors.initials: A
authors.lastname: Sobhani
authors.affiliation: Institute of Neuroanatomy, RWTH Aachen University, Aachen, Germany. azendedel@ukaachen.de.
authors.forename: Adib
authors.initials: A
authors.lastname: Zendedel
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002066:Busulfan / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D055101:Semen Analysis; D015398:Signal Transduction; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
chemical_list: C512808:DAZL protein, rat; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C489504:Tnp2 protein, rat; D016179:Granulocyte Colony-Stimulating Factor; C091974:Ddx4 protein, rat; D053487:DEAD-box RNA Helicases; D002066:Busulfan; D001973:Bromodeoxyuridine
keywords: Busulfan; Granulocyte colony-stimulating factor; Infertility; Spermatogenesis