title: Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava.
abstract: Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies.
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Quecine
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Thiago Falda
authors.initials: TF
authors.lastname: Leite
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Andressa Peres
authors.initials: AP
authors.lastname: Bini
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Thais
authors.initials: T
authors.lastname: Regiani
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Lívia Maria
authors.initials: LM
authors.lastname: Franceschini
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Ilara Gabriela Frasson
authors.initials: IG
authors.lastname: Budzinski
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Felipe Garbelini
authors.initials: FG
authors.lastname: Marques
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Mônica Teresa Veneziano
authors.initials: MT
authors.lastname: Labate
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Guidetti-Gonzalez
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: David Henry
authors.initials: DH
authors.lastname: Moon
authors.affiliation: Departament of Genetics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba -SP, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Labate
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000378:metabolism* / Q000472:pathogenicity*; D002853:Chromatography, Liquid / Q000379:methods; D005052:Eucalyptus / Q000382:microbiology*; D005656:Fungal Proteins / Q000145:classification / Q000378:metabolism; D058507:Host Specificity; D013058:Mass Spectrometry / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D020543:Proteome / Q000145:classification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D031647:Psidium / Q000382:microbiology*; D013045:Species Specificity; D013172:Spores, Fungal / Q000378:metabolism*; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D020543:Proteome