title: [Study of Mycoplasma pirum infection and related factors among male HIV/AIDS patients].
abstract: OBJECTIVE
  To investigate the infection status of Mycoplasma pirum among male HIV/AIDS patients in Jiangsu and analyze the risk factors. The genome sequencing of Mycoplasma pirum was completed for the first time.
  
  
  METHODS
  Male HIV infected individuals and AIDS patients confirmed in Jiangsu province were enrolled for 4 repeated cross-sectional studies by means of detecting the first flow urine sample and venous blood sample collected and questionnaire survey after informed consent. Genome sequencing was conducted for Mycoplasma pirum by using Illumina Hiseq 2000 sequencing platform.
  
  
  RESULTS
  A total of 1 541 HIV/AIDS patients were surveyed in this study. The infection rates of Mycoplasma pirum was 15.4%. The patients who received no HAART had higher risk to be infected with Mycoplasma pirum (OR = 1.344, 95% CI: 1.008-1.792). Otherwise, high CD4⁺T counts was a protective factor for Mycoplasma pirum infection (OR = 0.600, 95% CI: 0.444-0.810). Based on the sequencing result, the genome size of Mycoplasma pirum was 850 704 bp, the GC content was 24.21% the genome contained 708 genes, the total length of genes was 734 085 bp, the average length was 1 037 bp, accounting for 86.29% of genome.
  
  
  CONCLUSIONS
  More attention should be paid to the high infection rate of Mycoplasma pirum among male HIV/AIDS patients in the future AIDS prevention and control. The first genome sequencing of standard Mycoplasma pirum strain was completed in this study (registering serial number: AZHZ00000001), which can provide evidence for the further research of gene function and pathogenic mechanism of Mycoplasma pirum.
authors.affiliation: School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Lusi
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Jianru
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Public Health, Southeast University, Nanjing 210009, China; Email: wangbeilxb@seu.edu.cn.
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.affiliation: Jiangsu Provincial Centers for Diseases Control and Prevention.
authors.forename: Jinshui
authors.initials: J
authors.lastname: Xu
authors.affiliation: Jiangsu Provincial Centers for Diseases Control and Prevention.
authors.forename: Xiping
authors.initials: X
authors.lastname: Huan
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000382:microbiology*; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D002681:China / Q000453:epidemiology; D060085:Coinfection / Q000453:epidemiology*; D003430:Cross-Sectional Studies; D015658:HIV Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008297:Male; D009174:Mycoplasma; D009175:Mycoplasma Infections / Q000453:epidemiology*; D012307:Risk Factors; D011795:Surveys and Questionnaires