title: Synthesis of Depo-Medrol-chitosan hydrogel as new drug slow-release appliance and investigation of release kinetics by high-performance liquid chromatography.
abstract: The present study deals with preparation and optimization of a novel chitosan hydrogel-based matrix by suspension cross-linking method for controlled release of Depo-Medrol. The controlled release of Depo-Medrol for effective Rheumatoid arthritis disease has become an imperative field in the drug delivery system. In this context, it was intended to optimize loading circumstances by experimental design and also study the release kinetics of Depo-Medrol entrapped in the chitosan matrix in order to obtain maximal efficiency for drug loading. The optimum concentrations of chitosan (2.5 g), glutaraldehyde (3.05 μL) and Depo-Medrol (0.1 mg) were set up to achieve the highest value of drug loaded and the most sustained release from the chitosan matrix. In vitro monitoring of drug release kinetic using high-performance liquid chromatography showed that 73% of the Depo-Medrol was released within 120 min, whereas remained drug was released during the next 67 h. High correlation between first-order and Higuchi's kinetic models indicates a controlled diffusion of Depo-Medrol through the surrounding media. Moreover, recovery capacity >82% and entrapment efficiency of 58-88% were achieved under optimal conditions. Therefore, the new synthesized Depo Medrol-chitosan is an applicable appliance for arthritis therapy by slow release mechanism. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Qom, Qom, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh
authors.affiliation: Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.|Digestive Disease Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sara Majd
authors.initials: SM
authors.lastname: Jabbari
authors.affiliation: Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.|Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Amir H
authors.initials: AH
authors.lastname: Zarnani
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296, Gothenburg, Sweden.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Barghi
mesh_terms: D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003692:Delayed-Action Preparations; D020100:Hydrogels; D008775:Methylprednisolone / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D000077555:Methylprednisolone Acetate
chemical_list: D003692:Delayed-Action Preparations; D020100:Hydrogels; D000077555:Methylprednisolone Acetate; D048271:Chitosan; D008775:Methylprednisolone
keywords: chitosan; controlled release; depo-medrol; experimental design; high-performance liquid chromatography; kinetic model