title: Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China.
abstract: Bioelectrochemical systems (BESs) are promising technologies for energy and product recovery coupled with wastewater treatment, and the core microbial community in electrochemically active biofilm in BESs remains controversy. In the present study, 7 anodic communities from 6 bioelectrochemical systems in 4 labs in southeast, north and south-central of China are explored by 454 pyrosequencing. A total of 251,225 effective sequences are obtained for 7 electrochemically active biofilm samples at 3% cutoff level. While Alpha-, Beta-, and Gamma-proteobacteria are the most abundant classes (averaging 16.0-17.7%), Bacteroidia and Clostridia are the two sub-dominant and commonly shared classes. Six commonly shared genera i.e., Azospira, Azospirillum, Acinetobacter, Bacteroides, Geobacter, Pseudomonas, and Rhodopseudomonas dominate the electrochemically active communities and are defined as core genera. A total of 25 OTUs with average relative abundance >0.5% were selected and designated as core OTUs, and some species relating to these OTUs have been reported electrochemically active. Furthermore, cyclic voltammetry and chronoamperometry tests show that two strains from Acinetobacter guillouiae and Stappia indica, bacteria relate to two core OTUs, are electrochemically active. Using randomly selected bioelectrochemical systems, the study has presented extremely diverse bacterial communities in anodic biofilms, though, we still can suggest some potentially microbes for investigating the electrochemical mechanisms in bioelectrochemical systems.
authors.affiliation: Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of SciencesXiamen, China; College of Environmental Science and Engineering, Hunan UniversityChangsha, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of SciencesXiamen, China; College of Environmental Science and Engineering, Hunan UniversityChangsha, China.
authors.forename: Song
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of SciencesXiamen, China; College of Environmental Science and Engineering, Hunan UniversityChangsha, China.
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Zhang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Environment, Tsinghua University Beijing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liang
authors.affiliation: School of Environment, Tsinghua University Beijing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Environmental Science and Engineering, Hunan University Changsha, China.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Yang
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University Tainan, Taiwan.
authors.forename: I-Son
authors.initials: IS
authors.lastname: Ng
authors.affiliation: Department of Chemical and Materials Engineering, National I-Lan University I-Lan, Taiwan.
authors.forename: Bor-Yann
authors.initials: BY
authors.lastname: Chen
authors.affiliation: Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
keywords: bioelectrochemical systems; electrochemically active microorganisms; electron transfer; high-throughput sequencing; microbial community; microbial fuel cells