title: Rab23 is overexpressed in human bladder cancer and promotes cancer cell proliferation and invasion.
abstract: Rab23 overexpression has been implicated in several human cancers. However, its expression pattern and biological roles in human bladder cancer have not been elucidated. In this study, we examined Rab23 expression in 93 bladder cancer specimens and analyzed its correlation with clinicopathological parameters. We found that Rab23 was overexpressed in 45 of 93 (48.3 %) cancer specimens. Significant association was found between Rab23 overexpression and tumor invasion depth (p = 0.0027). Rab23 overexpression also negatively correlated with FGFR3 protein expression (p = 0.021). We found that Rab23 expression was lower in normal bladder transitional cell line SV-HUC-1 than in bladder cancer cell lines BIU-87, 5637, and T24. We knocked down Rab23 expression in T24 cancer cells and transfected a Rab23 plasmid in the BIU-87 cell line. Rab23 depletion inhibited cell growth rate and invasion, while its overexpression resulted in increased cell growth and invasion. In addition, we demonstrated that Rab23 depletion decreased and its transfection upregulated expression of cyclin E, c-myc, and MMP-9. Furthermore, we showed that Rab23 knockdown inhibited NF-κB signaling and its overexpression upregulated NF-κB signaling. BAY 11-7082 (NF-κB inhibitor) partly inhibited the effect of Rab23 on cyclin E and MMP-9 expression. In conclusion, the present study demonstrated that Rab23 overexpression facilitates malignant cell growth and invasion in bladder cancer through the NF-κB pathway.
authors.affiliation: Department of Urology, The First affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Yuanjun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Urology, The First affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Radiotherapy, The First affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Radiotherapy, The First affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Chuyang
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Radiotherapy, The First affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.affiliation: Department of Urology, The First affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhi
authors.affiliation: Department of Radiotherapy, The First affiliated Hospital of China Medical University, Shenyang, 110001, China. qiaojiang120@126.com.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Qiao
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D016328:NF-kappa B; C486614:RAB23 protein, human; D020691:rab GTP-Binding Proteins
keywords: Bladder cancer; Invasion; Proliferation; Rab23