title: Arabinogalactan:β-glucan as novel biodegradable carriers for recombinant human thrombin.
abstract: The aim of this work was to evaluate the effects of incorporating thrombin in arabinogalactan (AG)/β-glucan (BG)-based carriers. The products were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction (XRPD) and X-ray photoelectron spectroscopy techniques. Results, especially deconvoluted XRPD patterns indicated creation of new phases and potential complex formation. Results also highlighted that the AG carrier leads to higher residual thrombin-specific activity, while the in vivo haemostatic effect was enhanced when insoluble BG was present in the matrix. Our results confirm that thrombin can be successfully added to the carriers and that these materials are promising alternatives to standard vehicles.
authors.affiliation: a R&D Department , EUROFINS Bel/Novamann Ltd. , Nové Zámky , Slovak Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Veverka
authors.affiliation: b Department of Galenical Development , hameln rds a.s. , Modra , Slovak Republic.|c Faculty of Pharmacy, Department of Galenic Pharmacy , Comenius University , Bratislava , Slovak Republic.
authors.forename: Andrej
authors.initials: A
authors.lastname: Murányi
authors.affiliation: d Faculty of Chemical and Food Technology , Slovak Technical University , Bratislava , Slovak Republic.
authors.forename: Dušan
authors.initials: D
authors.lastname: Bakoš
authors.affiliation: e Department of Pharmacology , hameln rds a.s. , Modra , Slovak Republic.
authors.forename: Ján
authors.initials: J
authors.lastname: Kochan
authors.affiliation: d Faculty of Chemical and Food Technology , Slovak Technical University , Bratislava , Slovak Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Jorík
authors.affiliation: f Polymer Institute , Slovak Academy of Sciences , Bratislava , Slovak Republic.
authors.forename: Mária
authors.initials: M
authors.lastname: Omastová
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D005685:Galactans / Q000737:chemistry; D006487:Hemostasis / Q000187:drug effects; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D033362:Powder Diffraction; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage; D017550:Spectroscopy, Fourier Transform Infrared; D013917:Thrombin / Q000008:administration & dosage*; D014961:X-Ray Diffraction; D047071:beta-Glucans / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D005685:Galactans; D011994:Recombinant Proteins; D047071:beta-Glucans; D013917:Thrombin; C513462:recombinant human thrombin; C005653:arabinogalactan
keywords: Arabinogalactan; complexation, polymeric drug carrier; haemostasis; line profile analysis; spectroscopic characterization; thrombin; β-glucan