title: Age-dependent detection of erythrocytes glucose-6-phosphate dehydrogenase and its correlation with oxidative stress.
abstract: BACKGROUND
  Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH).
  
  
  OBJECTIVE
  This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6PD activity pattern was correlated with reduced glutathione (GSH) and total antioxidant potential in terms of FRAP (ferric reducing ability of plasma) value.
  
  
  METHODS
  We analyzed normal, healthy subjects of both sexes between the ages of 20 and 80 years. G6PD activity was determined by Burties method.
  
  
  RESULTS
  We observe a significant age-dependent decrease in G6PD activity (p < 0.0001). It was positively correlated with GSH (r = 0.5706) and total antioxidant potential (r = 0.7723) as a function of human age.
  
  
  CONCLUSIONS
  Our findings on erythrocyte G6PD and their correlation with GSH and FRAP provide evidence of a higher oxidative stress in old age population.
authors.affiliation: a Interdisciplinary Laboratory for Clinical Neuroscience (LiNC) , Department of Psychiatry, Universidade Federal de Sao Paulo - UNIFESP , Sao Paulo , Brazil .|b Amity Institute of Biotechnology , Amity University Uttar Pradesh , Noida, Uttar Pradesh , India , and.
authors.forename: Pawan Kumar
authors.initials: PK
authors.lastname: Maurya
authors.affiliation: b Amity Institute of Biotechnology , Amity University Uttar Pradesh , Noida, Uttar Pradesh , India , and.
authors.forename: Prabhanshu
authors.initials: P
authors.lastname: Kumar
authors.affiliation: c Department of Biosciences and Bioengineering , Indian Institute of Technology - Guwahati , Guwahati , Assam , India.
authors.forename: Pranjal
authors.initials: P
authors.lastname: Chandra
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D005954:Glucosephosphate Dehydrogenase; D005978:Glutathione
keywords: Aging; G6PD; erythrocytes; glutathione; oxidative stress