title: miR-204 regulates the EMT by targeting snai1 to suppress the invasion and migration of gastric cancer.
abstract: miR-204 was found to be downregulated in gastric cancer (GC) tissues, and the effect of miR-204 function on gastric cancer remains as a mystery. Therefore, this study was aimed at investigating the potential role of miR-204 involved in GC progression. Tissues collected from 60 gastric cancer patients were selected as the case group, while the matched normal paracancer tissues as controls. miR-204 expression levels in tissues and GC cells were detected using real-time fluorescent quantitative PCR. Luciferase assay was adopted to validate the interaction between potential gene targets and miR-204. Transwell assay was performed to evaluate the metastasis of GC cells. By building the epithelial-mesenchymal transition (EMT) model in vitro through the addition of transforming growth factor beta 1 (TGF-β1), expressions of miR-204 and snai1 in the EMT model together with their respective effects on EMT were evaluated. miR-204 was significantly downregulated in GC tissues and invasive GC cells (P < 0.05). The over-expression of miR-204 or downregulation of snai1 could significantly inhibit the metastasis and invasion of GC cells both in vitro and in vivo. The upregulated miR-204 expression or inhibited snai1 expression could suppress the EMT process in EMT in vitro models. Our study provided evidence that miR-204 may suppress the metastasis and invasion of GC cells through the regulation of the EMT process by targeting snai1.
authors.affiliation: Department of Pancreatic Surgery, First Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pancreatic Surgery, First Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Long
authors.affiliation: Department of Otorhinolaryngology, Fengtian Hospital, Shenyang Medical University, Shenyang, 110001, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Du
authors.affiliation: Department of Pancreatic Surgery, First Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Ge
authors.affiliation: Department of Pancreatic Surgery, First Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Kejian
authors.initials: K
authors.lastname: Guo
authors.affiliation: Department of Pancreatic Surgery, First Hospital of China Medical University, Shenyang, 110001, China. liuzhe4321@yeah.net.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000071250:Snail Family Transcription Factors / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
chemical_list: C549404:MIRN204 microRNA, human; D035683:MicroRNAs; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors
keywords: EMT; Gastric cancer; Invasion; Migration; Snai1; miR-204