title: Massively-multicellular alignment with the self-aggregate of air bubbles.
abstract: This study proposes a cell manipulation method with aggregated air bubbles on cell culture medium. This method requires no additional regents nor devices, except for normal cell-culture materials such as cell culture dishes and pipettes. Bubbles generated by pipetting were spontaneously aggregated with regularity on the whole surface and used as a mask for avoiding cell adhesion after cell-seeding. The diameter of bubbles was able to be controlled by the size of micro-pipette tips. Seeded cells spread to the whole area along the bubble gap. This technique is a surface-tension-driven self-assembly-based method. Using this technique, millions of living cells were successfully aligned into a hexagonal pattern within 300 μm in pattern width on the whole surface of dish for less than 2 h.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Haraguchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
mesh_terms: D000388:Air; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods
chemical_list: D003470:Culture Media