title: The effect of high pressure on the intracellular trehalose synthase activity of Thermus aquaticus.
abstract: To understand the effect of high pressure on the intracellular trehalose synthase activity, Thermus aquaticus (T. aquaticus) in the logarithmic growth phase was treated with high-pressure air, and its intracellular trehalose synthase (TSase) activity was determined. Our results indicated that pressure is a factor strongly affecting the cell growth. High pressure significantly attenuated the growth rate of T. aquaticus and shortened the duration of stationary phase. However, after 2 h of culture under 1.0 MPa pressure, the activity of intracellular TSase in T. aquaticus reached its maximum value, indicating that pressure can significantly increase the activity of intracellular TSase in T. aquaticus. Thus the present study provides an important guide for the enzymatic production of trehalose.
authors.affiliation: School of Bioengineering, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology, Jinan, 250353, People's Republic of China. yongsheng69@163.com.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Dong
authors.affiliation: School of Bioengineering, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology, Jinan, 250353, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Bioengineering, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology, Jinan, 250353, People's Republic of China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Duan
mesh_terms: D003593:Cytoplasm / Q000201:enzymology; D005285:Fermentation; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D018698:Glutamic Acid / Q000494:pharmacology; D011312:Pressure; D013824:Thermus / Q000201:enzymology* / Q000254:growth & development; D014199:Trehalose / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D018698:Glutamic Acid; D014199:Trehalose; D005964:Glucosyltransferases; C099692:trehalose synthase
keywords: Activity; High pressure; Thermus aquaticus; Trehalose synthase