title: Bacterial diversity of Grenache and Carignan grape surface from different vineyards at Priorat wine region (Catalonia, Spain).
abstract: Epiphytic bacteria on grape berries play a critical role in grape health and quality, which decisively influence the winemaking process. Despite their importance, the bacteria related with grape berry surface remain understudied and most previous work has been based on culture-dependent methods, which offer a limited view of the actual diversity. Herein, we used high-throughput sequencing to investigate the bacterial diversity on the surface from two grape varieties, Grenache and Carignan, and compared them across five vineyards included within the Priorat region (Spain). We could detect up to 14 bacterial phyla with Firmicutes (37.6% Bacillales and 14% Lactobacillales), Proteobacteria (16.8% Pseudomonadales and 11.6% Enterobacteriales) and Actinobacteria (3.4% Actinomycetales) being the most abundant. Bacterial community was different at each vineyard being grape varietal, geographical situation and orientation related with changes in bacterial populations. The most abundant bacterial taxa and those driving differences between the vineyards and grape varietals were identified. This study indicates that bacterial community heterogeneities can be influenced by geographic factors like orientation.
authors.affiliation: Biotecnología Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, C/ Marcel·lí Domingo, 1. 43007 Tarragona, Catalonia, Spain. Electronic address: carmen.portillo@urv.cat.
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: Portillo
authors.affiliation: Biotecnología Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, C/ Marcel·lí Domingo, 1. 43007 Tarragona, Catalonia, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Franquès
authors.affiliation: Biotecnología Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, C/ Marcel·lí Domingo, 1. 43007 Tarragona, Catalonia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Araque
authors.affiliation: Biotecnología Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, C/ Marcel·lí Domingo, 1. 43007 Tarragona, Catalonia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Reguant
authors.affiliation: Biotecnología Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, C/ Marcel·lí Domingo, 1. 43007 Tarragona, Catalonia, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Bordons
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D000068536:Bacillota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005638:Fruit / Q000382:microbiology*; D005843:Geography; D064307:Microbiota; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Bacterial diversity; Grape surface; Next generation sequencing; Vineyard orientation