title: PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice.
abstract: Chlorophyll (Chl) b is a ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. This pigment is synthesized from Chl a by chlorophyllide a oxygenase and plays a key role in adaptation to various environments. This study characterizes a rice mutant, pale green leaf (pgl), and isolates the gene PGL by using a map-based cloning approach. PGL, encoding chlorophyllide a oxygenase 1, is mainly expressed in the chlorenchyma and activated in the light-dependent Chl synthesis process. Compared with wild-type plants, pgl exhibits a lower Chl content with a reduced and disorderly thylakoid ultrastructure, which decreases the photosynthesis rate and results in reduced grain yield and quality. In addition, pgl exhibits premature senescence in both natural and dark-induced conditions and more severe Chl degradation and reactive oxygen species accumulation than does the wild-type. Moreover, pgl is sensitive to heat stress.
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.
authors.forename: Yaolong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Leng
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Dai
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Rao
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Tu
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Guangheng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China dalizeng@126.com qianqian188@hotmail.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.affiliation: State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China dalizeng@126.com qianqian188@hotmail.com.
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
mesh_terms: D002734:Chlorophyll / Q000096:biosynthesis; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003624:Darkness; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D012275:Oryza / Q000201:enzymology* / Q000254:growth & development* / Q000528:radiation effects; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000201:enzymology* / Q000254:growth & development* / Q000528:radiation effects / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D010105:Oxygenases; C115272:chlorophyll a oxygenase
keywords: Chlorophyll b; chlorophyllide a oxygenase; leaf senescence; pale green leaf; rice.