title: Proteomics screening of adenosine triphosphate-interacting proteins in the liver of diazinon-treated rats.
abstract: OBJECTIVE
  Diazinon (DZN) is one of the most important organophosphorus compounds used to control pests in agriculture in many countries. Several studies have shown that exposure to DZN may alter protein expression in the liver. In order to further investigate the mechanism of DZN toxicity, differentially expressed ATP-interacting proteins, following subacute exposure to toxin, were separated and identified in rat liver.
  
  
  METHODS
  Male rats were equally divided into four groups: control (corn oil) and DZN (15 mg/kg) by gavage once a day for 4 weeks. After homogenization of liver tissue, lysates were incubated ATP-sepharose beads. After several washes, ATP-interacting proteins were eluted and separated on 2-D polyacrylamide gels. Deferentially expressed proteins were cut and identified using matrix-assisted laser desorption/ionization/time-of-flight and Mascot database. Identified proteins were classified according to their biological process using protein analysis through evolutionary relationships (PANTHER) Web site.
  
  
  RESULTS
  In this work, we showed that several key proteins involved in biological processes such as antioxidant system, oxidative stress, apoptosis, and metabolism were differentially expressed after subacute exposure to DZN.
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Pourtaji
authors.affiliation: Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: R Yazdian
authors.initials: RY
authors.lastname: Robati
authors.affiliation: Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Lari
authors.affiliation: Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Hosseinzadeh
authors.affiliation: Nanotechnology Research Center, Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ramezanim@mums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Ramezani
authors.affiliation: Pharmaceutical Research Center, Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran abnouskh@mums.ac.ir.
authors.forename: K
authors.initials: K
authors.lastname: Abnous
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002846:Chromatography, Affinity; D003976:Diazinon / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D017208:Rats, Wistar; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C030548:ATP-sepharose; D007306:Insecticides; D020543:Proteome; D000255:Adenosine Triphosphate; D012685:Sepharose; D003976:Diazinon
keywords: ATP-interacting proteins; Affinity chromatography; diazinon; proteomics