title: Genome-Wide Linkage Mapping of QTL for Adult-Plant Resistance to Stripe Rust in a Chinese Wheat Population Linmai 2 × Zhong 892.
abstract: Stripe rust is one of the most devastating diseases of wheat (Triticum aestivum) worldwide. Adult-plant resistance (APR) is an efficient approach to provide long-term protection of wheat from the disease. The Chinese winter wheat cultivar Zhong 892 has a moderate level of APR to stripe rust in the field. To determine the inheritance of the APR resistance in this cultivar, 273 F6 recombinant inbred lines (RILs) were developed from a cross between Linmai 2 and Zhong 892. The RILs were evaluated for maximum disease severity (MDS) in two sites during the 2011-2012, 2012-2013 and 2013-2014 cropping seasons, providing data for five environments. Illumina 90k SNP (single nucleotide polymorphism) chips were used to genotype the RILs and their parents. Composite interval mapping (CIM) detected eight QTL, namely QYr.caas-2AL, QYr.caas-2BL.3, QYr.caas-3AS, QYr.caas-3BS, QYr.caas-5DL, QYr.caas-6AL, QYr.caas-7AL and QYr.caas-7DS.1, respectively. All except QYr.caas-2BL.3 resistance alleles were contributed by Zhong 892. QYr.caas-3AS and QYr.caas-3BS conferred stable resistance to stripe rust in all environments, explaining 6.2-17.4% and 5.0-11.5% of the phenotypic variances, respectively. The genome scan of SNP sequences tightly linked to QTL for APR against annotated proteins in wheat and related cereals genomes identified two candidate genes (autophagy-related gene and disease resistance gene RGA1), significantly associated with stripe rust resistance. These QTL and their closely linked SNP markers, in combination with kompetitive allele specific PCR (KASP) technology, are potentially useful for improving stripe rust resistances in wheat breeding.
authors.affiliation: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.|Department of Plant Genetics & Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.|International Maize and Wheat Improvement Center (CIMMYT) China Office, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: He
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.affiliation: Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Bai
authors.affiliation: College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
authors.forename: Weie
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Plant Genetics & Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, China.
authors.forename: Chaojie
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Xia
mesh_terms: D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D023281:Genomics; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000276:immunology
chemical_list: D005819:Genetic Markers