title: miR-34c-3p inhibits cell proliferation, migration and invasion of hepatocellular carcinoma by targeting MARCKS.
abstract: Recent studies have shown that microRNA-34c-3p (miR-34c-3p) is down-regulated in various types of cancers and involved in tumor growth, invasion and metastasis. However, the roles of miR-34c-3p in hepatocellular carcinoma (HCC) are poorly understood. In this study, the expression profile of miR-34c-3pin HCC tissues and cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR). The correlations of miR-34c-3p expression and clinicopathological characteristics were analyzed. The biological role of MiR-34c-3pin cell proliferation, migration and invasion was examined. In addition, the targets of miR-34c-3p were identified. The results showed that miR-34c-3p expression was significantly down-regulated in HCC tissues and cell lines; low expression level of miR-34c-3p was correlated with vascular invasion and advanced TNM stage. In vitro functional assays showed that overexpression of miR-34c-3pin HepG2 and Huh7 cells significantly reduced cell proliferation, migration and invasion. Furthermore, target analysis and luciferase assay identified myristoylated alanine-rich protein kinase c substrate (MARCKS) as a specific target of miR-34c-3p. Knockdown of MARCKS in HepG2 cells reduced cell migration and invasion, but not cell proliferation. Taken together, our findings implicate the potential application of miR-34c-3p as a tumor suppressor in cancer therapy.
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Yongyun
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Hui
authors.affiliation: Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University Yinchuan 750004, PR China.
authors.forename: Zuozheng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology*; D009367:Neoplasm Staging
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C000619710:MARCKS protein, human; C522425:MIRN34 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D000076250:Myristoylated Alanine-Rich C Kinase Substrate
keywords: MARCKS; MiR-34c-3p; hepatocellular carcinoma; tumor suppressor