title: Ligustilide treatment promotes functional recovery in a rat model of spinal cord injury via preventing ROS production.
abstract: Ligustilide from traditional Chinese medicine extract, angelica sinensis is one of the main active components, and has many pharmacological activities related to the effectiveness. This study sought to determine whether neuro-protection of ligustilide promotes functional recovery in a rat model of spinal cord injury (SCI) via preventing ROS production. Male Sprague-Dawley (SD) rats were induced using operation for model SCI. Furthermore, Basso, Beattie, Bresnahan (BBB) scale and footprint analysis of gait was used to assess the neuro-protection of ligustilide on SCI. The intracellular reactive oxygen species (iROS), prostaglandin E(2) (PGE(2)), interleukin-1β (IL-1β) and tumor necrosis factor (TNF)-α production levels were measured by monoclonal enzyme immunoassay kit. Inducible nitric oxide synthase (iNOS) gene expression, activator protein-1 (AP-1) and c-Jun N-terminal kinase (JNK) protein expressions were detected using Quantitative real-time reverse transcription polymerase chain reaction (Q-PCR) and western blot analyses, respectively. Interestingly, treatment with ligustilide significantly increased BBB scale and reduced recovery of coordination in SCI rats. After SCI, the iROS, PGE(2), IL-1β, TNF-α production levels and iNOS gene expression were significantly suppressed in SCI rats. These results suggest that the neuro-protection of ligustilide promotes functional recovery in a rat model of spinal cord injury via preventing ROS production.
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei, P. R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei, P. R. China.
authors.forename: Aixi
authors.initials: A
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei, P. R. China.
authors.forename: Danli
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei, P. R. China.
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Xu
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D029971:Angelica sinensis; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D008517:Phytotherapy / Q000379:methods*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D020127:Recovery of Function / Q000187:drug effects*; D013119:Spinal Cord Injuries / Q000473:pathology*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C027820:ligustilide; D015107:4-Butyrolactone
keywords: Ligustilide; ROS; spinal cord injury