title: miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression.
abstract: MicroRNA-34a (miR-34a) functions as a tumor suppressor gene and inhibits abnormal cell growth by regulating the expression of other genes. The role of miR-34a in regulating sirtuin 1 (SIRT1) in prostate cancer remains unclear. The objective of the present study was to investigate the biological function and molecular mechanisms of miR-34a regulation of SIRT1 in human prostate cancer samples and the human prostate cancer cell line, PC-3. Fresh prostate tissues were obtained from patients, and the miR-34a expression in prostate cancer tissues was measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). qPCR and western blotting were performed to assess the effects of miR-34a overexpression on SIRT1 regulation in PC-3 cells, and the cell growth was assessed by Cell Counting Kit-8 (CCK-8). Flow cytometry was used to assess the cell cycle status of the cells. The miR-34a expression levels in prostate cancer tissues were significantly reduced compared with adjacent normal prostate tissues (P<0.05). SIRT1 expression levels in PC-3 cells with over-expression of miR-34a were significantly reduced compared with those in the negative control (P<0.05). The over-expression of miR-34a inhibited PC-3 cells growth and resulted in increased cell cycle arrest compared with the negative control (P<0.05). In conclusion, miR-34a inhibits the human prostate cancer cell proliferation, in part, through the downregulation of SIRT1 expression.
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Duan
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Ge
authors.affiliation: Department of Urology Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.
authors.forename: Xue-Pei
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: Department of Stem Cell and Organ Transplantation Laboratory, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Wu
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Jin-Shun
authors.initials: JS
authors.lastname: Feng
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Chen
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: L I
authors.initials: LI
authors.lastname: Zhang
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Zhi-Hao
authors.initials: ZH
authors.lastname: Yuan
authors.affiliation: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Chao-Hong
authors.initials: CH
authors.lastname: Fu
keywords: cell proliferation; human prostate cancer cell; microRNA-34a; sirtuin 1