title: MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (CKD). In the present study, we elucidated the role of microRNA 205 (miR-205) in cisplatin-induced renal cell apoptosis and explored the molecular mechanisms.
  
  
  METHODS
  The chronic interstitial nephropathy rat model was induced, and the miRNA expression profile in the kidney cells from rats with CKD was screened. Cisplatin-induced apoptosis in normal renal HK-2 cells was evaluated using flow cytometry, and regulation of miR-205 on target gene was validated using luciferase assay, western blot and real time PCR assays.
  
  
  RESULTS
  We found that miR-205 expression was significantly decreased in the cells from kidney of CKD rat (P<0.01). Our data showed that when miR-205 was overexpressed or silenced using the mimic or inhibitor, the percentages of apoptotic cells were suppressed or increased significantly (P<0.05), respectively. Moreover, we have identified CMTM4 gene, which is involved in cell proliferation and apoptosis, as a novel target for miR-205. In addition, miR-205 could inhibit apoptosis by binding to the 3'UTR of CMTM4 mRNA and inhibiting its transcriptional activity.
  
  
  CONCLUSIONS
  This study elucidated that miR-205 plays an important role in the regulation of apoptosis in renal cells, suggesting a potential therapeutic target to hinder CKD development.
authors.affiliation: Department of Internal Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, 257034, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, 257034, P. R. China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, 257034, P. R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Internal Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, 257034, P. R. China.
authors.forename: Linna
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, 257034, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
keywords: Apoptosis; CMTM4; Chronic kidney disease; microRNA 205