title: Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma.
abstract: Periostin (POSTN) is an extracellular matrix protein (ECM) critical for epithelial-mesenchymal transitions (EMT) in several kinds of tumor cells. Previous studies have indicated that EMT exists in craniopharyngioma (CP), and expression of POSTN is a significant factor in the prognosis of CP. However, it has never been explored whether POSTN exists in CP, or how it activates CP's EMT. The expression of POSTN was examined in adamantinomatous craniopharyngioma (ACP) primary cells and tissues by immunohistochemistry, PCR and Western blot, respectively. The effects and mechanisms of POSTN on ACP cells' EMT were also analyzed. It was found that POSTN expression increased in ACP-associated fibroblasts. Overexpressed POSTN significantly elevated the EMT of ACP cells by regulating the expression of associated genes. More importantly, our further study revealed that the upregulated POSTN activated Akt signaling pathway to regulate the EMT. This study showed that POSTN is responsible for the EMT of ACP cells, and POSTN might be a potential molecular therapeutic target for ACP treatment in future.
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Neurosurgery, Fujian Provincial Hospital, Fuzhou 350001, China.
authors.forename: Shi-hao
authors.initials: SH
authors.lastname: Zheng
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: chenmingsjwk@163.com.
authors.forename: Song-tao
authors.initials: ST
authors.lastname: Qi
mesh_terms: D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D003397:Craniopharyngioma / Q000378:metabolism* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: Craniopharyngioma; Epithelial–mesenchymal transition; Periostin; Prognosis