title: Effect of cyclophosphamide on fungal infection in SLE mice detected by fluorescent quantitative PCR.
abstract: OBJECTIVE
  To observe the effect of the cyclophosphamide (CTX) on systemic lupus erythematosus mice by fluorescent quantitative polymerase chain reaction.
  
  
  METHODS
  (BALB/c × C57BL/J6) F1 hybrid mice were used as recipients of donor lymphocytes which were injected intravenously. Mice were randomly divided into control group and treatment group, serum autoantibody and urinary protein were measured, renal pathological changes and the situation of fungal infection were observed.
  
  
  RESULTS
  The reduced urinary protein, ameliorated renal lesions were found in the CTX-treated mice. Candida albicans and Aspergillus flavus were detected respectively in respiratory tract and gastrointestinal tract.
  
  
  CONCLUSIONS
  CTX can alleviate lupus nephritis and the fluorescent quantitative PCR is a rapid and sensitive method for the detection of fungal infection.
authors.affiliation: Shandong Provincial Hospital for Skin Diseases, Shandong University, Jinan 250022, Shandong, China; Shandong Provincial Institute of Dermatology and Venereology, Shandong Academy of Medical Sciences, Jinan 250022, Shandong, China; Department of Dermatology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China.
authors.forename: Hong-Fen
authors.initials: HF
authors.lastname: Ge
authors.affiliation: Department of Dermatology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China. Electronic address: 18661807818@163.com.
authors.forename: Guan-Zhi
authors.initials: GZ
authors.lastname: Chen
authors.affiliation: Department of Dermatology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Pan
authors.affiliation: Laboratory Department, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
keywords: Animal model; Fungus; Polymerase chain reaction; Systemic lupus erythematosus