title: A simple and rapid ultra-high-performance liquid chromatography-tandem mass spectrometry method to determine plasma biotin in hemodialysis patients.
abstract: A simple, rapid, and selective method for determination of plasma biotin was developed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). After single-step protein precipitation with methanol, biotin and stable isotope-labeled biotin as an internal standard (IS) were chromatographed on a pentafluorophenyl stationary-phase column (2.1 × 100 mm, 2.7 μm) under isocratic conditions using 10 mm ammonium formate-acetonitrile (93:7, v/v) at a flow rate of 0.6 mL/min. The total chromatographic runtime was 5 min for each injection. Detection was performed in a positive electrospray ionization mode by monitoring selected ion transitions at m/z 245.1/227.0 and 249.1/231.0 for biotin and the IS, respectively. The calibration curve was linear in the range of 0.05-2 ng/mL using 300 μL of plasma. The intra- and inter-day precisions were all <7.1%. The accuracy varied from -0.7 to 8.2%. The developed UHPLC-MS/MS method was successfully applied to determine plasma biotin concentrations in hemodialysis patients. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Fuso Pharmaceutical Industries Ltd Research and Development Center, 2-3-30, Morinomiya, Jotoku, Osaka, 536-8523, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Yagi
authors.affiliation: Fuso Pharmaceutical Industries Ltd Research and Development Center, 2-3-30, Morinomiya, Jotoku, Osaka, 536-8523, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Nishizawa
authors.affiliation: Laboratory of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Sciences, 6-3, Aramaki-aza-aoba, Aobaku, Sendai, 980-8578, Japan.
authors.forename: Itiro
authors.initials: I
authors.lastname: Ando
authors.affiliation: Koujinkai Nagamachi Clinic, 2-8-2, Happonmatsu, Taihakuku, Sendai, 982-0001, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Oguma
authors.affiliation: Laboratory of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Sciences, 6-3, Aramaki-aza-aoba, Aobaku, Sendai, 980-8578, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Sato
authors.affiliation: Department of Planning for Drug Development and Clinical Evaluation, Tohoku University Graduate School of Pharmaceutical Sciences, 6-3, Aramaki-aza-aoba, Aobaku, Sendai, 980-8578, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Imai
authors.affiliation: Department of Planning for Drug Development and Clinical Evaluation, Tohoku University Graduate School of Pharmaceutical Sciences, 6-3, Aramaki-aza-aoba, Aobaku, Sendai, 980-8578, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Fujiwara
mesh_terms: D001710:Biotin / Q000097:blood*; D002138:Calibration; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D006435:Renal Dialysis; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001710:Biotin
keywords: UHPLC-MS/MS; biotin; hemodialysis; human plasma