title: Comparative genome analysis of a norovirus GII.4 strain GZ2013-L10 isolated from South China.
abstract: In this study, the genome sequence of a norovirus GII.4 strain isolated from South China was comparatively analyzed. The RNA genome of the strain GZ2013-L10 was composed of 7513 nucleotides. Phylogenetic analyses based on three ORFs confirmed its genotype as GII.Pe/GII.4-2012. Compared with other 22 genomes of the same variant, nine distinct nucleotide substitutions were found in the new genome, which resulted in three amino acid changes. All 138 capsid protein VP1 sequences of GII.4-2012 variants were also collected, and multiple alignments revealed 35 variable codons. Evolutionary analyses of GII.4-2012 variants were performed against previous pandemic GII.4 variants, and 2 distinctive changes were identified on epitopes A and E (E368, T413), which resulted in an obvious variation of their solvent-accessible surface areas. Therefore, the genome of GZ2013-L10 was extensively characterized, and new emerging variations on viral epitopes were predicted to contribute to NoV persistence in humans.
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, People's Republic of China.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xue
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, People's Republic of China.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, People's Republic of China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Cai
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, People's Republic of China. wuqp203@163.com.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, People's Republic of China. wuqp203@163.com.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, People's Republic of China.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, People's Republic of China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Kou
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, People's Republic of China.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, People's Republic of China.
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, People's Republic of China.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, People's Republic of China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Guo
mesh_terms: D002681:China; D016679:Genome, Viral; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Capsid protein VP1; Comparative analysis; GII.4-2012; Genome; Homology modeling; Norovirus