title: Transforming growth factor-β1 promotes homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury.
abstract: BACKGROUND
  Acute ischemia reperfusion-induced kidney injury is a common cause of acute renal failure, and it is also an important cause of delayed recovery of transplanted kidney functions and even loss of function. However, there is no effective treatment method in clinical applications presently.
  
  
  OBJECTIVE
  The objective was to investigate effects of transforming growth factor-β1 on homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury.
  
  
  METHODS
  Effects of TGF-β1 over-expression in MSCs on expression of CXCR4 and chemotactic effect to SDF-1 were investigated by in vitro transmembrane chemotaxis. Anti-TGF-β1 antibody was incubated with ischemia reperfusion injury renal tissue homogenate and effects of anti-TGF-β1 antibody were observed. In addition, effects of TGF-β1 gene transfection and anti-CXCR4 antibody treatment in MSCs on expression of SDF-1/CXCR4 axis of renal tissues and damage repair were further explored.
  
  
  RESULTS
  Expression of TGF-β1 mRNA in the IRI group increased significantly, and MSCs transplantation could enhance expression of CXCR4 mRNA in rats of the IRI group, the expression of CXCR4 can be decreased by the anti-TGF-β1 antibody and the anti-CXCR4 antibody. TGF-β1 induced homing of MSCs in repair of renal ischemic reperfusion injury by regulating expression of CXCR4 on cell membranes. Blue fluorescence of DAPI-positive MSCs cells of renal parenchyma in the IRI+MSC group was enhanced significantly, which was significantly inhibited by anti-TGF-β1 and anti-CXCR4 antibody, and the inhibitory effect of anti-CXCR4 antibody was more obvious than that of anti-TGF-β1 antibody.
  
  
  CONCLUSIONS
  Transforming growth factor-β1 promotes homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury, which will provide useful data on role of TGF-β1 in regulating SDF-1/CXCR4 axis-induced MSCs homing.
authors.affiliation: Department of Nephrology, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei Province, P. R. China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Si
authors.affiliation: Department of Orthopaedics Surgery, Wuhan General Hospital of Guangzhou Command Wuhan 430070, Hubei Province, P. R. China.
authors.forename: Ximing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Nephrology, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei Province, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Nephrology, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei Province, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wu
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods; D002465:Cell Movement / Q000502:physiology*; D004195:Disease Models, Animal; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D015427:Reperfusion Injury / Q000378:metabolism*; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: C486264:Cxcr4 protein, rat; D019718:Receptors, CXCR4; D053773:Transforming Growth Factor beta1
keywords: SDF-1/CXCR4 axis; Transforming growth factor-β1; bone marrow mesenchymal stem cells; homing; renal ischemia-reperfusion injury