title: Bovine Herpes Virus 1 Major Immediate Early Transcription Unit 1 (IETU-1) Uses Alternative Promoters to Transcribe BICP0 and BICP4 Transcripts.
abstract: Immediate early (IE) genes are transcribed immediately after infection in BHV1 from two different immediate early transcription units. It is reported that the immediate early transcription unit I (IE TU1) of Bovine herpesvirus 1 (BHV1) transcribes two proteins BICP0 and BICP4 from a single promoter by alternative splicing but with identical 5'UTR. We found that the transcripts of BICP0 and BICP4 have different 5'UTRs. The bioinformatics analysis shows two similar spatially arranged TATA less promoter for the two transcripts. The bioinformatics analysis also showed a similar promoter for the IE TU2 which transcribes BICP22. The data strongly suggest that BICP0 and BICP4 are transcribed from two different promoters. The transcript produced by each promoter is spliced specifically as opposed to what has been reported earlier. The BICP0 and BICP4 also show different levels of expression. The expression level of BICP4 continuously declines after attaining a peak level at 1 h, while BICP0 shows biphasic expression supporting the earlier observation that it is expressed from two different promoters.
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, 243122, India.
authors.forename: Mayank
authors.initials: M
authors.lastname: Pokhriyal
authors.affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, U.P, 211007, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Verma
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, 243122, India.
authors.forename: Barkha
authors.initials: B
authors.lastname: Ratta
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, 243122, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, 243122, India.
authors.forename: Meeta
authors.initials: M
authors.lastname: Saxena
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, 243122, India. bhaskar@ivri.res.in.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D020506:Untranslated Regions; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D015534:Trans-Activators; D020506:Untranslated Regions; D014759:Viral Envelope Proteins; C083786:immediate early protein BICP4, bovine herpesvirus 1; D044767:Ubiquitin-Protein Ligases; C086729:bICP0 protein, Bovine herpesvirus 1