title: New insight into multifunctional role of peroxiredoxin family protein: Determination of DNA protection properties of bacterioferritin comigratory protein under hyperthermal and oxidative stresses.
abstract: Bacterioferritin comigratory protein (BCP) is a monomeric conformer acting as a putative thiol-dependent bacterial peroxidase, however molecular basis of DNA-protection via DNA-binding has not been clearly understood. In this study, we characterized the DNA binding properties of BCP using various lengths and differently shaped architectures of DNA. An electrophoretic mobility shift assay and electron microscopy analysis showed that recombinant TkBCP bound to DNA of a circular shape (double-stranded DNA and single-stranded DNA) and a linear shape (16-1000 bp) as well as various architectures of DNA. In addition, DNA protection experiments indicated that TkBCP can protect DNA against hyperthermal and oxidative stress by removing highly reactive oxygen species (ROS) or by protecting DNA from thermal degradation. Based on these results, we suggest that TkBCP is a multi-functional DNA-binding protein which has DNA chaperon and antioxidant functions.
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, South Korea. Electronic address: taeinlee2011@kangwon.ac.kr.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, South Korea.
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Chung
authors.affiliation: Advanced Nano Surface Research Group, Korea Basic Science Institute, 169-148 Gwahak-ro, Daejeon, 305-333, South Korea.
authors.forename: Hyung Joong
authors.initials: HJ
authors.lastname: Yun
authors.affiliation: Advanced Nano Surface Research Group, Korea Basic Science Institute, 169-148 Gwahak-ro, Daejeon, 305-333, South Korea.
authors.forename: Jonghan
authors.initials: J
authors.lastname: Won
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, South Korea. Electronic address: hsjung@kangwon.ac.kr.
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Jung
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004249:DNA Damage; D018869:Heat-Shock Response; D006358:Hot Temperature; D018384:Oxidative Stress; D011485:Protein Binding; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; C068491:bacterioferritin comigratory protein, Bacteria; D004247:DNA
keywords: Bacterioferritin comigratory protein; DNA chaperone; DNA-binding protein; Peroxiredoxin; Reactive oxygen species