title: Adjuvanted influenza-H1N1 vaccination reveals lymphoid signatures of age-dependent early responses and of clinical adverse events.
abstract: Adjuvanted vaccines afford invaluable protection against disease, and the molecular and cellular changes they induce offer direct insight into human immunobiology. Here we show that within 24 h of receiving adjuvanted swine flu vaccine, healthy individuals made expansive, complex molecular and cellular responses that included overt lymphoid as well as myeloid contributions. Unexpectedly, this early response was subtly but significantly different in people older than ∼35 years. Wide-ranging adverse clinical events can seriously confound vaccine adoption, but whether there are immunological correlates of these is unknown. Here we identify a molecular signature of adverse events that was commonly associated with an existing B cell phenotype. Thus immunophenotypic variation among healthy humans may be manifest in complex pathophysiological responses.
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London, UK.|ImmunoSurveillance Laboratory, Francis Crick Institute, Lincoln's Inn Laboratories, London, UK.|Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.
authors.forename: Olga
authors.initials: O
authors.lastname: Sobolev
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London, UK.|Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.|Rheumatology and Translational Immunology Research Laboratories (LaRIT), Division of Rheumatology, IRCCS Policlinico San Matteo/University of Pavia, Pavia, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Binda
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London, UK.|ImmunoSurveillance Laboratory, Francis Crick Institute, Lincoln's Inn Laboratories, London, UK.|Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.
authors.forename: Sean
authors.initials: S
authors.lastname: O'Farrell
authors.affiliation: Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.
authors.forename: Anna
authors.initials: A
authors.lastname: Lorenc
authors.affiliation: Momenta Pharmaceuticals, Cambridge, Massachusetts, USA.
authors.forename: Joel
authors.initials: J
authors.lastname: Pradines
authors.affiliation: Momenta Pharmaceuticals, Cambridge, Massachusetts, USA.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Momenta Pharmaceuticals, Cambridge, Massachusetts, USA.
authors.forename: Jay
authors.initials: J
authors.lastname: Duffner
authors.affiliation: Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.|Department of Genetics, King's College London, London, UK.
authors.forename: Reiner
authors.initials: R
authors.lastname: Schulz
authors.affiliation: Department of Infectious Diseases, King's College London, London, UK.
authors.forename: John
authors.initials: J
authors.lastname: Cason
authors.affiliation: Health Protection Agency, Porton Down, Salisbury, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Zambon
authors.affiliation: Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.|Department of Infectious Diseases, King's College London, London, UK.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Malim
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London, UK.|Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Peakman
authors.affiliation: Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.|Academic Department of Rheumatology, Centre for Molecular and Cell Biology of Inflammation, King's College London, UK.
authors.forename: Andrew
authors.identifier: http://orcid.org/0000-0003-0470-1827
authors.initials: A
authors.lastname: Cope
authors.affiliation: Momenta Pharmaceuticals, Cambridge, Massachusetts, USA.
authors.forename: Ishan
authors.initials: I
authors.lastname: Capila
authors.affiliation: Momenta Pharmaceuticals, Cambridge, Massachusetts, USA.
authors.forename: Ganesh V
authors.initials: GV
authors.lastname: Kaundinya
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London, UK.|ImmunoSurveillance Laboratory, Francis Crick Institute, Lincoln's Inn Laboratories, London, UK.|Biomedical Research Centre at Guy's and St Thomas' Hospital and King's College London, London, UK.
authors.forename: Adrian C
authors.initials: AC
authors.lastname: Hayday
mesh_terms: D000276:Adjuvants, Immunologic; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D015551:Autoimmunity; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016000:Cluster Analysis; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000009:adverse effects / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000378:metabolism* / Q000517:prevention & control; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism; D010641:Phenotype; D013997:Time Factors; D059467:Transcriptome; D014611:Vaccination; D055815:Young Adult
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001323:Autoantibodies; D016207:Cytokines; D007252:Influenza Vaccines