title: An evaluation of the effect of pulsed wave low-level laser therapy on the biomechanical properties of the vertebral body in two experimental osteoporosis rat models.
abstract: Osteoporosis (OP) increases vertebral fragility as a result of the biomechanical effects of diminished bone structure and composition. This study has aimed to assess the effects of pulsed wave low-level laser therapy (PW LLLT) on cancellous bone strength of an ovariectomized (OVX-d) experimental rat model and a glucocorticoid-induced OP (GIOP) experimental rat model. There were four OVX-d groups and four dexamethasone-treated groups. A group of healthy rats was used for baseline evaluations. The OVX-d rats were further subdivided into the following groups: control rats with OP, OVX-d rats that received alendronate, OVX-d rats treated with PW LLLT, and OVX-d rats treated with alendronate and PW LLLT. The remaining rats received dexamethasone and were divided into four groups: control, alendronate-treated rats, laser-treated rats, and laser-treated rats with concomitant administration of alendronate. PW LLLT (890 nm, 80 Hz, 0.972 J/cm(2)) was performed on the spinal processes of the T12, L1, L2, and L3 vertebras. We extracted the L1 vertebrae and submitted them to a mechanical compression test. Biomechanical test findings showed positive effects of the PW LLLT and alendronate administration on increasing bending stiffness and maximum force of the osteoporotic bones compared to the healthy group. However, laser treatment of OVA-d rats significantly increased stress high load compared to OVA-d control rats. PW LLLT preserved the cancellous (trabecular) bone of vertebra against the detrimental effects of OV-induced OP on bone strength in rats compared to control OV rats.
authors.affiliation: Cellular and Molecular Biology Research Centre, Shahid Beheshti University of Medical Sciences, Po Box 19395/4719, 1985717443, Tehran, Iran. bayat_m@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
authors.affiliation: Department of Anatomy, Medical School, Zanjan University of Medical Sciences, Zanjan, Iran. fredoni_javad@yahoo.com.
authors.forename: Mohammadjavad
authors.initials: M
authors.lastname: Fridoni
authors.affiliation: Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hosseinnejati1992@yahoo.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Nejati
authors.affiliation: Department of Anatomical Sciences and Biology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. a.mostafavinia@gmail.com.
authors.forename: Atarodalsadat
authors.initials: A
authors.lastname: Mostafavinia
authors.affiliation: Department of Anatomical Sciences and Biology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. m.salimi87@yahoo.com.
authors.forename: Maryam
authors.initials: M
authors.lastname: Salimi
authors.affiliation: Cellular and Molecular Biology Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. mahdi.samani.2020@gmail.com.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Ghatrehsamani
authors.affiliation: Department of Anatomical Sciences and Biology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. m_amin58@yahoo.com.
authors.forename: Mohammad-Amin
authors.initials: MA
authors.lastname: Abdollahifar
authors.affiliation: Department of Anatomical Sciences and Biology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. azamnajar@yahoo.com.
authors.forename: Azam
authors.initials: A
authors.lastname: Najar
authors.affiliation: Medical School, Arak University of Medical Sciences, Arak, Iran. sababayat@gmail.com.
authors.forename: Saba
authors.initials: S
authors.lastname: Bayat
authors.affiliation: Cellular and Molecular Biology Research Centre, Shahid Beheshti University of Medical Sciences, Po Box 19395/4719, 1985717443, Tehran, Iran. journalistbest@gmail.com.
authors.forename: Fatemesadat
authors.initials: F
authors.lastname: Rezaei
mesh_terms: D019386:Alendronate / Q000627:therapeutic use; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D005938:Glucocorticoids / Q000009:adverse effects; D028022:Low-Level Light Therapy; D008297:Male; D055595:Mechanical Phenomena; D010024:Osteoporosis / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology* / Q000532:radiotherapy*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013131:Spine / Q000187:drug effects / Q000503:physiopathology* / Q000528:radiation effects*
chemical_list: D005938:Glucocorticoids; D019386:Alendronate
keywords: Alendronate; Biomechanical properties; Cancellous bone; Glucocorticoid administration; Low-level laser therapy; Lumbar vertebra; Osteoporosis; Ovariectomy; Rat