title: c-Myc-activated long non-coding RNA H19 downregulates miR-107 and promotes cell cycle progression of non-small cell lung cancer.
abstract: To verify c-Myc can regulate the expression of lncRNA H19 directly in non-small cell lung cancer (NSCLC) and clarify the molecular mechanism on how lncRNA H19 promote the cell cycle progression of NSCLC. The mRNA levels of lncRNA H19 in NSCLC tissues and cells, the adjacent tissues and normal cells were determined by RT-PCR. The expression change of lncRNA H19 in NSCLC cells after transfection with pcDNA3.1-c-Myc or c-Myc-siRNA was determined by RT-PCR, respectively. Targeted role of c-Myc on the promoter of H19 was studied by luciferase reporter assay. Chromosome immune coprecipitation (ChIP) was used to confirm the relationship between c-Myc and H19. MiRNAs that have base-pairing with H19 was predicted by online software. The relationship between H19 and miR-107 was determined by disturbing and overexpressing the expression of H19. The influence of the changes of H19 and miR-107 on cell cycle progression was determined by flow cytometry. The mRNA levels of lncRNA H19 in NSCLC tissues and cells were significantly higher than the adjacent tissues and normal cells, respectively. The expression of H19 increased or decreased accordingly with the overexpression and knockdown of c-Myc. The activity of the promoter of H19 was strengthened by c-Myc. While the expression of miR-107 increased or decreased with the overexpression and knockdown of H19, respectively. The number of cells in G2/M stage decreased significantly with the knockdown of H19 and miR-107 compared with the control group. Our study demonstrates that lncRNA H19, which is induced by c-Myc, is up-regulated in NSCLC. H19 influences the mitotic progression of NSCLC cell lines.
authors.affiliation: Department of Oncology, 303 Hospital of Chinese People's Liberation Army Nanning 530021, P.R. China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Oncology, 303 Hospital of Chinese People's Liberation Army Nanning 530021, P.R. China.
authors.forename: Juanmei
authors.initials: J
authors.lastname: Mo
authors.affiliation: Department of Oncology, 303 Hospital of Chinese People's Liberation Army Nanning 530021, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Chemotherapy, Affiliated Tumor Hospital of Guangxi Medical University Nanning 530021, P.R. China.
authors.forename: Qitao
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Chemotherapy, Affiliated Tumor Hospital of Guangxi Medical University Nanning 530021, P.R. China.
authors.forename: Shaozhang
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Oncology, 303 Hospital of Chinese People's Liberation Army Nanning 530021, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Oncology, 303 Hospital of Chinese People's Liberation Army Nanning 530021, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, 303 Hospital of Chinese People's Liberation Army Nanning 530021, P.R. China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Luo
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: C094335:H19 long non-coding RNA; C549045:MIRN107 microRNA, human; C489427:MYC protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding
keywords: LncRNA H19; MiR-107; NSCLC; c-Myc; cell cycle