title: DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole.
abstract: The DNA binding property of 5,10,15-Tris(4-carboxyphenyl) corrole (TCPC) was studied by UV-Visible, fluorescence and circular dichroism (CD) spectroscopic methods. TCPC can bind to ct-DNA via an outside binding mode with the binding constant of Kb = 1.05 × 10⁵ M(-1). TCPC also displayed good photonuclease activity, which involves singlet oxygen species (¹O₂). The binding constant between TCPC and human serum albumin (HSA) is KA = 2.24 × 10⁵ M(-1) with a simulated binding distance of 2.06 nm. The fluorescence quenching of HSA by TCPC followed a static quenching process. Site marker competitive displacement experiments indicated that warfarin site I is the main binding site. The secondary structure of HSA was changed upon interaction with TCPC, which was confirmed by UV-Visible and CD spectroscopy.
authors.affiliation: Department of Kidney Transplantation, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. nngg20102009@hotmail.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Na
authors.affiliation: Department of Kidney Transplantation, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. zhaodaq@mail.sysu.edu.cn.
authors.forename: Da-Qiang
authors.initials: DQ
authors.lastname: Zhao
authors.affiliation: Department of Kidney Transplantation, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. midget201086@gmail.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hepatic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. njiang163@163.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, South China University of Technology, Guangzhou 510640, China. wjy1990scut@163.com.
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Wen
authors.affiliation: Department of Chemistry, South China University of Technology, Guangzhou 510640, China. chhyliu@scut.edu.cn.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Liu
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D006801:Humans; D015394:Molecular Structure; D055668:Photochemical Processes; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011166:Porphyrins; D012709:Serum Albumin; C431863:corrole; D004247:DNA
keywords: DNA; corrole; human serum albumin; photonuclease activity