title: Overproduction of recombinant human bone morphogenetic protein-7 in Chinese hamster ovary cells.
abstract: Bone morphogenetic protein-7 is a multifunctional growth factor involved in various cellular processes such as osteogenesis, kidney and eye development, brown adipogenesis, and bone metastasis, and thus has been considered to have therapeutic potential for treating various diseases. In this study, we established a Chinese hamster ovary (CHO) cell line stably overexpressing recombinant human BMP-7 (rhBMP-7). Over the course of a 14-day fed-batch culture process in a 7.5-l bioreactor (5-l working volume) using chemically defined medium, the established cells could produce over 188 mg/l of rhBMP-7 protein. The rhBMP-7 was purified to homogeneity from the culture supernatant using a two-step chromatographic procedure that resulted in a recovery rate of approximately 55%, with protein purity greater than 95%. The purified rhBMP-7 was further demonstrated to be functionally active by measuring the proliferation of MC3T3-E1 cells, revealing a half-maximal effective concentration of 28.31 ng/ml.
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Sena
authors.initials: S
authors.lastname: Yoon
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Jia
authors.initials: J
authors.lastname: Pi
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Yongsu
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Kwangehee
authors.initials: K
authors.lastname: Baek
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Yongin-si, Gyeonggi-do 446-701, Republic of Korea. Electronic address: jsyoon@khu.ac.kr.
authors.forename: Jaeseung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000818:Animals; D019149:Bioreactors; D055419:Bone Morphogenetic Protein 7; D016466:CHO Cells; D002845:Chromatography; D003001:Cloning, Molecular; D003412:Cricetulus / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D011994:Recombinant Proteins
keywords: Bioreactor process; Bone morphogenetic protein-7 (BMP-7); Chinese hamster ovary (CHO) cells; Recombinant protein expression; Stable cell line