title: Effect of Short Chain Poly(ethylene glycol)s on the Hydration Structure and Dynamics around Human Serum Albumin.
abstract: We report the changes in the hydration dynamics around a globular protein, human serum albumin (HSA), in the presence of two short chain crowding agents, namely poly(ethylene glycol)s (PEG 200 and 400). The change in the network water structure is investigated using FTIR spectroscopy in the far-infrared (FIR) frequency range. Site specific changes are obtained by time-resolved fluorescence spectroscopic technique using the intrinsic fluorophore tryptophan (Trp214) of HSA. The collective hydration dynamics of HSA in the presence of PEG molecules are obtained using terahertz (THz) time domain spectroscopy (TTDS) and high intensity p-Ge THz measurements. Our study affirms a considerable perturbation of HSA hydration beyond a critical concentration of PEG.
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences , Block JD, Sector III, Salt Lake, Kolkata 700098, India.
authors.forename: Nirnay
authors.initials: N
authors.lastname: Samanta
authors.affiliation: Department of Physical Chemistry II, Ruhr-University Bochum , 44780 Bochum, Germany.
authors.forename: Trung Quan
authors.initials: TQ
authors.lastname: Luong
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences , Block JD, Sector III, Salt Lake, Kolkata 700098, India.
authors.forename: Debasish
authors.initials: D
authors.lastname: Das Mahanta
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences , Block JD, Sector III, Salt Lake, Kolkata 700098, India.
authors.forename: Rajib Kumar
authors.initials: RK
authors.lastname: Mitra
authors.affiliation: Department of Physical Chemistry II, Ruhr-University Bochum , 44780 Bochum, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Havenith
mesh_terms: D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D055068:Terahertz Spectroscopy; D014364:Tryptophan / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D012709:Serum Albumin; D012996:Solutions; D014867:Water; D011092:Polyethylene Glycols; D014364:Tryptophan; C000595213:polyethylene glycol 400