title: Boiogito Increases the Metabolism of Fatty Acids in Proximal Tubular Cells through Peroxisome Proliferators-Activated Receptor (PPAR) α Agonistic Activity.
abstract: The promotion of fatty acid metabolism, to which peroxisome proliferators-activated receptor (PPAR) α contributes, has been suggested to participate in maintaining the function of renal proximal tubular epithelial cells (PTECs). The loading of fatty acids to PTECs could result in cell inflammation and cell death. A "Kampo" medicine, Boiogito (BO), is used to treat overweight women exhibiting chronic fatigue and edema in the lower extremities or knees. BO improves renal function by reducing the portion of fatty acids, thereby preventing damage to PTECs. In this study, BO and Astragalus Root (AsR), a constituent crude drug of BO, were administered orally to intravenously bovine serum albumin (BSA)-administered mice to evaluate the PPARα-cAMP responsive element binding protein (CREB) binding protein (CBP) complex binding activity and/or mRNA expression of PPARα, as quantified by enzyme-linked immunosorbent assay (ELISA) and/or polymerase chain reaction (PCR). Increases in PPARα-CBP complex binding activity and the expression of PPARα mRNA were observed not only in BO-administered mice but also in AsR-administered mice, accompanied by a decrease in the amount of renal fatty acid.
authors.affiliation: Department of Pharmacognosy, Tohoku Pharmaceutical University.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Wakana
authors.initials: W
authors.lastname: Matsuyama
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Arai
authors.forename: Saho
authors.initials: S
authors.lastname: Koizumi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Rie
authors.initials: R
authors.lastname: Tomioka
authors.forename: Kenroh
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D051379:Mice; D047493:PPAR alpha / Q000819:agonists* / Q000235:genetics / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D047493:PPAR alpha; C523704:boiogito; D000242:Cyclic AMP; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse