title: miRNA-149* promotes cell proliferation and suppresses apoptosis by mediating JunB in T-cell acute lymphoblastic leukemia.
abstract: MicroRNA-149* (miRNA-149*) functions as an oncogenic regulator in human melanoma. However, the effect of miRNA-149* on T-cell acute lymphoblastic leukemia (T-ALL) is unclear. Here we aimed to analyze the effects of miRNA-149* on in vitro T-ALL cells and to uncover the target for miRNA-149* in these cells. The miRNA-149* level was determined in multiple cell lines and bone marrow cells derived from patients with T-ALL, B acute lymphoblastic leukemia (B-ALL), acute myelocytic leukemia (AML), and healthy donors. We found that miRNA-149* was highly expressed in T-ALL cell lines and T-ALL patients' bone marrow samples. JunB was identified as a direct target of miR-149*. miRNA-149* mimics downregulated JunB levels in Molt-4 and Jurkat cells, while miRNA-149* inhibitors dramatically upregulated JunB expression in these cells. miRNA-149* mimics promoted proliferation, decreased the proportion of cells in G1 phase, and reduced cell apoptosis in T-ALL cells, while miRNA-149* inhibitors prevented these effects. miRNA-149* mimics downregulated p21 and upregulated cyclinD1, 4EBP1, and p70s6k in Molt-4 and Jurkat cells. Again, inhibitors prevented these effects. Our findings demonstrate that miRNA-149* may serve as an oncogenic regulator in T-ALL by negatively regulating JunB.
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Sheng-Jin
authors.initials: SJ
authors.lastname: Fan
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Hui-Bo
authors.initials: HB
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cui
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Kong
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Sun
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Zhao
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China. Electronic address: liyinghua1965@aliyun.com.
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics* / Q000276:immunology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: C581727:JunB protein, human; C551557:MIRN149 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors
keywords: Apoptosis; JunB; Proliferation; T-ALL; miRNA-149*