title: [β-Glucan promotes the maturation and migration of bone marrow-derived dendritic cells].
abstract: OBJECTIVE
  To investigate the effects of β-glucan on the maturation and migration of bone marrow-derived dendritic cells (BMDCs).
  
  
  METHODS
  BMDCs were isolated from mouse bone marrow cells in vitro and induced by β-glucan for maturation. The expressions of cell surface markers were detected by flow cytometry (FCM). The cytokines (IL-6, IL-12p40, tumor necrosis factor α) in the supernatants were measured by ELISA, and the expressions of intracellular CC chemokine receptor 1 (CCR1), CCR2, CCR5, CCR7 were determined by real-time quantitative PCR. Furthermore, the chemotactic response to CC chemokine ligand 19 (CCLl9) and CCL21, i.e. CCR7-1igands, was measured by Transwell(TM) migration assay. Moreover, the number of migrated cells in the draining lymph nodes was analyzed by FCM.
  
  
  RESULTS
  Compared with the control group, the expressions of co-stimulation molecules (MHC II, CD40, CD80, CD86) on BMDCs were up-regulated in the presence of β-glucan. Furthermore, β-glucan could prompt BMDCs to secret high levels of IL-6, TNF-α, IL-12 p40 and increase the production of CCR7 mRNA. After β-glucan treatment, BMDCs were more sensitive to CCL19/CCL21. The number of BMDCs migrated from subcutaneous injection site into the draining lymph nodes significantly increased in β-glucan group.
  
  
  CONCLUSIONS
  β-glucan can promote the maturation of BMDCs and enhance the migration ability of BMDCs in vitro and in vivo.
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Dongqin
authors.initials: D
authors.lastname: Xu
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Yongling
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ding
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Keqing
authors.initials: K
authors.lastname: Qian
authors.affiliation: Medical Research Center, Changzhou Second People's Hospital, Nanjing Medical University, Changzhou 213000, China. *Corresponding author, E-mail: qichunjian@njmu.edu.cn.
authors.forename: Chunjian
authors.initials: C
authors.lastname: Qi
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008810:Mice, Inbred C57BL; D019707:Receptors, Chemokine / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D019707:Receptors, Chemokine; D047071:beta-Glucans