title: The construction of customized nucleosomal arrays.
abstract: A simple, efficient, and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. In addition, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesize customized arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups, and reaction sites. This is, therefore, an enabling technology for the in vitro study of chromatin structure and function.
authors.affiliation: Biophysics Laboratories, School of Biology, University of Portsmouth, Portsmouth PO1 2DT, UK.
authors.forename: Chenyi
authors.initials: C
authors.lastname: Wu
authors.affiliation: Biophysics Laboratories, School of Biology, University of Portsmouth, Portsmouth PO1 2DT, UK.
authors.forename: Christopher
authors.initials: C
authors.lastname: Read
authors.affiliation: Biophysics Laboratories, School of Biology, University of Portsmouth, Portsmouth PO1 2DT, UK.
authors.forename: John
authors.initials: J
authors.lastname: McGeehan
authors.affiliation: Biophysics Laboratories, School of Biology, University of Portsmouth, Portsmouth PO1 2DT, UK. Electronic address: colyn.crane-robinson@port.ac.uk.
authors.forename: Colyn
authors.initials: C
authors.lastname: Crane-Robinson
mesh_terms: D003001:Cloning, Molecular; D009707:Nucleosomes / Q000235:genetics*
chemical_list: D009707:Nucleosomes
keywords: 30-nm fiber; 601 sequence; Chromatin; Nucleosome