title: Epigenetic down-regulated DDX10 promotes cell proliferation through Akt/NF-κB pathway in ovarian cancer.
abstract: Ovarian cancer contributes to the majority of ovarian cancer, while the molecular mechanisms remain elusive. Recently, some DEAD box protein 1 has been reported play a tumor suppressor role in ovarian cancer progression. However, the functions of DEAD box protein (DDX) members in ovarian cancer development remain largely unknown. In current study, we retrieved GEO databases and surprisingly found that DDX10 is significantly down-regulated in ovarian cancer tissues compared with normal ovary. These findings suggest that DDX10 might also play a suppressive role in ovarian cancer. We then validated the down-regulated expression pattern of DDX10 in fresh ovarian cancer tissues. Furthermore, both loss- and gain-functions assays reveal that the down-regulated DDX10 could promote ovarian cancer proliferation in vitro and the xenograft subcutaneous tumor formation assays confirmed these findings in vivo. In addition, we found that DDX10 is epigenetic silenced by miR-155-5p in ovarian cancer. Moreover, we further preliminary illustrated that down-regulated DDX10 promotes ovarian cancer cell proliferation through Akt/NF-κB pathway. Taken together, in current study, we found a novel tumor suppressor, DDX10, is epigenetic silenced by miR-155-5p in ovarian cancer, and the down-regulated expression pattern of DDX10 promotes ovarian cancer proliferation through Akt/NF-κB pathway. Our findings shed the light that DDX families might be a novel for ovarian cancer treatment.
authors.affiliation: Department of Gynaecology and Obstetrics, Dongying People's Hospital, No.317, East City South 1st Road, Dongying, Shandong, 257091, China.
authors.forename: Muhuizi
authors.initials: M
authors.lastname: Gai
authors.affiliation: Department of Gynaecology and Obstetrics, Dongying People's Hospital, No.317, East City South 1st Road, Dongying, Shandong, 257091, China.
authors.forename: Qifang
authors.initials: Q
authors.lastname: Bo
authors.affiliation: Department of Gynaecology and Obstetrics, Dongying People's Hospital, No.317, East City South 1st Road, Dongying, Shandong, 257091, China. Electronic address: lixiaqi_dph@sina.com.
authors.forename: Lixia
authors.initials: L
authors.lastname: Qi
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D044127:Epigenesis, Genetic / Q000235:genetics*; D005260:Female; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D016328:NF-kappa B; D051057:Proto-Oncogene Proteins c-akt; C499217:DDX10 protein, human; D053487:DEAD-box RNA Helicases
keywords: Akt/NF- NF-κB pathway; Cell proliferation; DDX10; miR-155-5p