title: Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro.
abstract: Campylobacter jejuni is the most prevalent cause of bacterial gastroenteritis worldwide. Polyphosphate kinases 1 and 2 (PPK1 and PPK2) regulate several cellular processes, including the biosynthesis of the bacterial cell wall. Despite their importance, whether PPK1 and PPK2 modulate the composition of C. jejuni outer membrane constituents (OMCs) and consequently impact its interaction with host cells remains unknown. Our comparative analysis between C. jejuni wild type, Δppk1, and Δppk2 strains showed qualitative and quantitative differences in the total OMC composition among these strains. Importantly, these OMC variations observed on the C. jejuni polyphosphate kinase mutants are directly related to their capacity to invade, survive, and alter the immune response of intestinal epithelial cells in vitro. Specifically, sub-fractionation of the C. jejuni OMC indicated that OMC proteins are uniquely associated with bacterial invasion, whereas C. jejuni OMC proteins, lipids, and lipoglycans are all associated with C. jejuni intracellular survival. This study provides new insights regarding the function of polyphosphate kinases and their role in C. jejuni infection.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research & Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH 44691, USA.
authors.forename: Ruby
authors.initials: R
authors.lastname: Pina-Mimbela
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jesús Arcos
authors.initials: JA
authors.lastname: Madrid
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research & Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH 44691, USA.
authors.forename: Anand
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH 43210, USA.|Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jordi B
authors.initials: JB
authors.lastname: Torrelles
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research & Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH 44691, USA.|Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Gireesh
authors.initials: G
authors.lastname: Rajashekara
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000166:cytology* / Q000187:drug effects / Q000472:pathogenicity*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology*; D005759:Gastroenteritis / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000378:metabolism; D058990:Molecular Targeted Therapy / Q000639:trends; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D016209:Interleukin-8; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase