title: Analysis of metabolic effects of menthol on WFS1-deficient mice.
abstract: In this study, we investigated the physiological regulation of energy metabolism in wild-type (WT) and WFS1-deficient (Wfs1KO) mice by measuring the effects of menthol treatment on the O2 consumption, CO2 production, rectal body temperature, and heat production. The basal metabolism and behavior was different between these genotypes as well as TRP family gene expressions. Wfs1KO mice had a shorter life span and weighed less than WT mice. The food and water intake of Wfs1KO mice was lower as well as the body temperature when compared to their WT littermates. Furthermore, Wfs1KO mice had higher basal O2 consumption, and CO2 and heat production than WT mice. In addition, Wfs1KO mice showed a higher response to menthol administration in comparison to WT mice. The strongest menthol effect was seen on different physiological measures 12 h after oral administration. The highest metabolic response of Wfs1KO mice was seen at the menthol dose of 10 mg/kg. Menthol increased O2 consumption, and CO2 and heat production in Wfs1KO mice when compared to their WT littermates. In addition, the expression of Trpm8 gene was increased. In conclusion, our results show that the Wfs1KO mice develop a metabolic phenotype characterized with several physiological dysfunctions.
authors.affiliation: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia marite.ehrlich@ut.ee.
authors.forename: Marite
authors.initials: M
authors.lastname: Ehrlich
authors.affiliation: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Marilin
authors.initials: M
authors.lastname: Ivask
authors.affiliation: Department of Physiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia Division of Pharmacology and Pharmacotherapy, Faculty of Pharmacy University of Helsinki, Helsinki, Finland.
authors.forename: Atso
authors.initials: A
authors.lastname: Raasmaja
authors.affiliation: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D008610:Menthol / Q000494:pharmacology*; D051379:Mice; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology*; D022722:Thermogenesis / Q000187:drug effects / Q000502:physiology*
chemical_list: D008565:Membrane Proteins; C114987:wolframin protein; D008610:Menthol
keywords: Menthol; Trpm8; Wfs1; metabolism