title: [miR-365 inhibits proliferation and promotes apoptosis of SOSP9607 osteosarcoma cells].
abstract: OBJECTIVE
  To investigate the effect of miR-365 on the proliferation and apoptosis in SOSP-9607 osteosarcoma cells.
  
  
  METHODS
  SOSP-9607 cells were transiently transfected with miR-365 mimic or miR-365 inhibitor which were artificially synthesized. The expression of miR-365 was detected by real-time PCR (qRT-PCR); the cell cycle and apoptosis was analyzed by flow cytometry; the cell proliferation was observed by MTT assay; and the level of KRAS was determined by qRT-PCR and Western blotting.
  
  
  RESULTS
  miR-365 mimic up-regulated the expression level of miR-365 in SOSP-9607 osteosarcoma cells. After miR-365 mimic transfection, the proliferation of SOSP-9607 cells was inhibited; the cell cycle was arrested in G1 phase; the apoptosis rate increased and the expression of KRAS was reduced in miR-365 mimic transfected cells. On the contrary, when miR-365 inhibitor was transfected into SOSP-9607 cells, the expression level of miR-365 was significantly reduced along with promoted cell proliferation, suppressed cell apoptosis and increased KRAS expression.
  
  
  CONCLUSIONS
  miR-365 could inhibit the proliferation and promote the apoptosis in SOSP-9607 osteosarcoma cells probably by mediating the expression of KRAS.
authors.affiliation: Department of Orthopaedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Jinjian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Xi
authors.affiliation: Department of neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Digestive Diseases, Navy General Hospital, Beijing 100037, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Hu
authors.affiliation: Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China. *Corresponding author, E-mail: fmmuwangt@fmmu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. *Corresponding author, E-mail: drshanlq@fmmu.edu.cn.
authors.forename: Lequn
authors.initials: L
authors.lastname: Shan
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C117307:KRAS protein, human; C546819:MIRN365 microRNA, human; D035683:MicroRNAs; D016283:Proto-Oncogene Proteins p21(ras)