title: Online solid-phase extraction with high-performance liquid chromatography and mass spectrometry for the determination of five tannins in traditional Chinese medicine injections.
abstract: A rapid analytical method based on online solid-phase extraction with high-performance liquid chromatography and mass spectrometry has been established and applied to the determination of tannin compounds that may cause adverse effects in traditional Chinese medicine injections. Different solid-phase extraction sorbents have been compared and the elution buffer was optimized. The performance of the method was verified by evaluation of recovery (≥40%), repeatability (RSD ≤ 6%), linearity (r(2) ≥ 0.993), and limit of quantification (≤0.35 μg/mL). Five tannin compounds, gallic acid, cianidanol, gallocatechin gallate, ellagic acid, and penta-O-galloylglucose, were identified with concentrations ranging from 3.1-37.4 μg/mL in the analyzed traditional Chinese medicine injections.
authors.affiliation: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Sicen
authors.initials: S
authors.lastname: Wang
mesh_terms: D001331:Automation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D008516:Medicine, Chinese Traditional; D052616:Solid Phase Extraction / Q000379:methods*; D013634:Tannins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D013634:Tannins
keywords: High-performance liquid chromatography; Mass spectrometry; Quality control; Solid-phase extraction; Tannins