title: Multi-gene engineering in plants with RNA-guided Cas9 nuclease.
abstract: The use of RNA-guided Cas9 endonuclease for the concurrent engineering of multiple genes has been demonstrated in a number of plant species. Although Cas9 is a large monomeric protein, the single guide RNA (sgRNA) that directs it to a specific DNA target sequence is small and easy to reprogram. It is therefore relatively simple to produce numerous sgRNAs to target multiple endogenous sequences. Several approaches to express multiple sgRNAs and Cas9 in plants for the purpose of simultaneous editing or transcriptional regulation of many genes have recently been reported.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Oleg
authors.initials: O
authors.lastname: Raitskin
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: nicola.patron@tsl.ac.uk.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Patron
mesh_terms: D000818:Animals; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering; D006801:Humans; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D000094704:RNA, Guide, CRISPR-Cas Systems
chemical_list: D004720:Endonucleases; D000094704:RNA, Guide, CRISPR-Cas Systems