title: Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T.
abstract: To obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherificaion (10BASE(d)-T). A remarkable color-changing and turning-on probe was selected from the library, and its physicochemical properties upon target-specific binding were obtained. Combination analyses of fluorescence emission titration, isothermal titration calorimetry (ITC), and quantitative saturation-transfer difference (STD) NMR measurements, followed by in silico docking simulation, rationalized the most plausible geometry of the ligand-protein interaction.
authors.affiliation: Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Taki
authors.affiliation: Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Inoue
authors.affiliation: Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Mochizuki
authors.affiliation: Department of Anesthesiology, University of Wisconsin, School of Medicine and Public Health , Madison, Wisconsin 53706 United States.
authors.forename: Jay
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Bioscience, Graduate School of Science and Engineering, Kagoshima University , 1-21-35 Korimoto, Kagoshima, Kagoshima 890-0065, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ito
mesh_terms: D017123:Bacteriophage T7 / Q000737:chemistry* / Q000378:metabolism; D003116:Color; D004987:Ethers / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D062105:Molecular Docking Simulation; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D019151:Peptide Library; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D004987:Ethers; D005456:Fluorescent Dyes; D008024:Ligands; D015335:Molecular Probes; D019151:Peptide Library; D013438:Sulfhydryl Compounds; D014764:Viral Proteins