title: Metabonomic study of the effects of different acupuncture directions on therapeutic efficacy.
abstract: Posterior circulation ischemia (PCI) is a common clinical ischemic cerebrovascular disease that can endanger the lives of patients in severe cases. Our previous research found that needling the Fengchi (GB20) acupoint presents a significant effect on PCI and that different acupuncture directions can exert different effects. To investigate the biological mechanism of acupuncture directions, rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based metabonomic techniques are used to analyze the metabolic profiles of urine samples. The urine samples were obtained from 30 healthy control subjects, 60 PCI patients before and after treatment of different acupuncture directions. Six metabolites, including LPE (22:6), estrone, uric acid, vanillylmandelic acid, N-acetyl-l-tyrosine, and 4-hydroxyphenylacetylglutamine were identified as potential biomarkers of acupuncture treatment of PCI. Acupuncture treatment of PCI patients significantly changed the levels of these potential biomarkers. Moreover, different acupuncture directions showed different effects on the contents of these biomarkers. These results strongly support the belief that acupuncture direction performs an important function in acupuncture intervention. The findings provide new insights into the mechanism of acupuncture treatment and reveal that acupuncture manipulation results in various curative.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ju
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Shizhe
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China.
authors.forename: Haoyue
authors.initials: H
authors.lastname: Deng
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: XiaoFeng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Si
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Linghui
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Tian
authors.affiliation: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China. Electronic address: tjmzh86@163.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. Electronic address: yuboli1@163.com.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Li
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000368:Aged; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D002545:Brain Ischemia / Q000378:metabolism* / Q000628:therapy* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged
chemical_list: D015415:Biomarkers
keywords: Acupuncture; Metabonomics; Posterior circulation ischemia; Rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry