title: [Cultivation and characterization of an ammonia oxidizing archaeon enriched from wastewater treatment plant].
abstract: OBJECTIVE
  To enrich ammonia-oxidizing archaeon (AOA) from wastewater treatment plants, identify its phylogenetic status, morphology and determine its growth and ammonia oxidation rates.
  
  
  METHODS
  AOA was enriched in autotrophic medium containing antibiotics by using consecutive passage. The purity, uniformity and phylogenetic status of archaeal enrichment were determined with different molecular tools. The morphology was determined with scanning electron microscopy. The AOA growth andammonia oxidation rates were calculated from the corresponding experimental results.
  
  
  RESULTS
  An AOA enrichment HJ-2b with purity of 93% was obtained. The similarity of its 16S rRNA gene with Nitrososphaera sp. JG1 was 100%, suggesting it belonged to Nitrososphaera spp., although the similarity of amoA gene between these two species was only 72%. HJ-2b cell was rod-shaped, with the maximum specific growth rate of 0. 43 d -1, the specific ammonia-oxidation rate of 3. 9 fmol/(cell . d).
  
  
  CONCLUSIONS
  HJ-2b has great significance in studying the occurrence and contribution of AOA in wastewater treatment.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Li
authors.forename: Xianghua
authors.initials: X
authors.lastname: Wen
authors.forename: Kun
authors.initials: K
authors.lastname: Ding
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D019641:DNA, Archaeal / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D018508:Water Purification / Q000295:instrumentation
chemical_list: D000641:Ammonia; D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D062065:Wastewater