title: Resveratrol Pretreatment Decreases Ischemic Injury and Improves Neurological Function Via Sonic Hedgehog Signaling After Stroke in Rats.
abstract: Resveratrol has neuroprotective effects for ischemic cerebral stroke. However, its neuroprotective mechanism for stroke is less well understood. Beneficial actions of the activated Sonic hedgehog (Shh) signaling pathway in stroke, such as improving neurological function, promoting neurogenesis, anti-oxidative, anti-apoptotic, and pro-angiogenic effects, have been noted, but relatively little is known about the role of Shh signaling in resveratrol-reduced cerebral ischemic injury after stroke. The present study tests whether the Shh pathway mediates resveratrol to decrease cerebral ischemic injury and improve neurological function after stroke. We observed that resveratrol pretreatment significantly improved neurological function, decreased infarct volume, enhanced vitality, and reduced apoptosis of neurons in vivo and vitro after stroke. Meanwhile, expression levels of Shh, Ptc-1, Smo, and Gli-1 mRNAs were significantly upregulated and Gli-1 was relocated to the nucleus. Intriguingly, in vivo and in vitro inhibition of the Shh signaling pathway with cyclopamine, a Smo inhibitor, completely reversed the above effects of resveratrol. These results suggest that decreased cerebral ischemic injury and improved neurological function by resveratrol may be mediated by the Shh signaling pathway.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Fanren
authors.initials: F
authors.lastname: Tang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Luling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016, Chongqing, China. xyqh200@126.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000008:administration & dosage; D002545:Brain Ischemia / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D005260:Female; D053823:Hedgehog Proteins / Q000096:biosynthesis*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013267:Stilbenes / Q000008:administration & dosage*; D020521:Stroke / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D053823:Hedgehog Proteins; D018696:Neuroprotective Agents; C546888:Shh protein, rat; D013267:Stilbenes; D000077185:Resveratrol
keywords: Ischemic injury; Neurological function; Resveratrol; Sonic hedgehog signaling; Stroke