title: Electrochemical label-free degranulation monitoring for in-situ evaluation of cellular function.
abstract: We fabricated a degranulation monitoring device, combining ion-sensitive field-effect transistor (ISFET) and microperfusion system. The electrical properties of ISFET were maintained even after immobilization of RBL-2H3 mast cells on the sensor. We successfully demonstrated in-situ monitoring of degranulation from stimulated RBL-2H3 cells by ionomycin. Potential change was induced by the release of acid-granule contents, which result in local pH decrease on the sensor under physiological conditions. This microdevice is expected to contribute as a platform technology for evaluating induced immune responses by chemical compounds.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tabata
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Goda
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyahara
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects; D004563:Electrochemistry / Q000379:methods*; D004867:Equipment Design; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D015759:Ionomycin / Q000494:pharmacology; D007477:Ions; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010477:Perfusion; D011199:Potentiometry; D051381:Rats; D013194:Staining and Labeling; D014173:Transistors, Electronic
chemical_list: D007477:Ions; D015759:Ionomycin; D002118:Calcium