title: Comparative Analysis of the Complete Genome of Lactobacillus plantarum GB-LP2 and Potential Candidate Genes for Host Immune System Enhancement.
abstract: Acute respiratory virus infectious diseases are a growing health problem, particularly among children and the elderly. Much effort has been made to develop probiotics that prevent influenza virus infections by enhancing innate immunity in the respiratory tract until vaccines are available. Lactobacillus plantarum GB-LP2, isolated from a traditional Korean fermented vegetable, has exhibited preventive effects on influenza virus infection in mice. To identify the molecular basis of this strain, we conducted a whole-genome assembly study. The single circular DNA chromosome of 3,284,304 bp was completely assembled and 3,250 proteinencoding genes were predicted. Evolutionarily accelerated genes related to the phenotypic trait of anti-infective activities for influenza virus were identified. These genes encode three integral membrane proteins, a teichoic acid export ATP-binding protein and a glucosamine - fructose-6-phosphate aminotransferase involved in host innate immunity, the nonspecific DNA-binding protein Dps, which protects bacteria from oxidative damage, and the response regulator of the three-component quorum-sensing regulatory system, which is related to the capacity of adhesion to the surface of the respiratory tract and competition with pathogens. This is the first study to identify the genetic backgrounds of the antiviral activity in L. plantarum strains. These findings provide insight into the anti-infective activities of L. plantarum and the development of preventive probiotics.
authors.affiliation: C&K Genomics, Seoul 08826, Republic of Korea.|Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Woori
authors.initials: W
authors.lastname: Kwak
authors.affiliation: C&K Genomics, Seoul 08826, Republic of Korea.
authors.forename: Kwondo
authors.initials: K
authors.lastname: Kim
authors.affiliation: Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.affiliation: Genebiotech Co. Ltd., Seoul 06774, Republic of Korea.
authors.forename: Chanho
authors.initials: C
authors.lastname: Lee
authors.affiliation: Genebiotech Co. Ltd., Seoul 06774, Republic of Korea.
authors.forename: Jungsun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Genebiotech Co. Ltd., Seoul 06774, Republic of Korea.
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sook Hee
authors.initials: SH
authors.lastname: Yoon
authors.affiliation: Department of Animal Resources Science, Dankook University, Cheonan 31116, Republic of Korea.
authors.forename: Dae-Kyung
authors.initials: DK
authors.lastname: Kang
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.affiliation: C&K Genomics, Seoul 08826, Republic of Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Heo
authors.affiliation: C&K Genomics, Seoul 08826, Republic of Korea.
authors.forename: Seoae
authors.initials: S
authors.lastname: Cho
mesh_terms: D019143:Evolution, Molecular; D005285:Fermentation; D016680:Genome, Bacterial; D006801:Humans; D055633:Immune System Phenomena / Q000235:genetics; D007113:Immunity, Innate; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D019936:Probiotics; D053038:Quorum Sensing / Q000235:genetics; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000382:microbiology; D014780:Viruses / Q000276:immunology
keywords: Lacatobacillus plantarum GB-LP2; anti-viral effect; comparative genomics; host immune enhancement