title: Characterization of Circular RNAs.
abstract: Accumulated lines of evidence reveal that a large number of circular RNAs are produced in transcriptomes from fruit fly to mouse and human. Unlike linear RNAs shaped with 5' cap and 3' tail, circular RNAs are characterized by covalently closed loop structures without open terminals, thus requiring specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs derived from intron lariats (ciRNAs) and circular RNAs produced from back spliced exons (circRNAs) in human.
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. liyang@picb.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. linglingchen@sibcb.ac.cn.|School of Life Science and Technology, ShanghaiTech University, Shanghai, 200031, China. linglingchen@sibcb.ac.cn.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Chen
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D005091:Exons; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D007438:Introns; D051379:Mice; D012313:RNA / Q000032:analysis* / Q000235:genetics* / Q000302:isolation & purification; D012326:RNA Splicing; D000079962:RNA, Circular; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017423:Sequence Analysis, RNA / Q000379:methods; D059467:Transcriptome
chemical_list: D000079962:RNA, Circular; D012313:RNA
keywords: Circular RNAs; RNA fractionation; RNase R; ciRNAs; circRNAs