title: Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood.
abstract: Staphylococcus epidermidis is frequently associated with the emergence of medical-device-associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blood and its cellular and soluble components is poorly understood. Herein, we evaluated biofilm structure, biofilm cells culturability and viability, and the transcription of a panel of genes associated with S. epidermidis biofilms virulence, upon interaction with whole human blood or plasma. Our results showed that although whole human blood caused significant alterations in biofilm structure and in the number of culturable and viable cells, plasma was the main regulator of the transcription of genes with central role in biofilm formation, maturation and immune evasion. These findings highlight the urgent need to intensify studies aiming to evaluate the impact of host soluble factors on S. epidermidis biofilms fitness and persistence.
authors.affiliation: CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Angela
authors.initials: A
authors.lastname: França
authors.affiliation: CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal nunocerca@ceb.uminho.pt.
authors.forename: Nuno
authors.initials: N
authors.lastname: Cerca
mesh_terms: D018533:Biomass; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D010949:Plasma; D013212:Staphylococcus epidermidis / Q000502:physiology*; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
keywords: Staphylococcus epidermidis; biofilms; gene expression; human blood; plasma; virulence genes