title: Cytological Study of Breast Carcinoma Before and After Oncotherapy with Special Reference to Morphometry and Proliferative Activity.
abstract: Our study was done to assess the cytological changes due to oncotherapy in breast carcinoma especially on morphometry and proliferative activity. Cytological aspirates were collected from a total of 32 cases of invasive ductal carcinoma both before and after oncotherapy. Morphometry was done on the stained cytological smears to assess the different morphological parameters of cell dimension by using the ocular morphometer and the software AutoCAD 2007. Staining was done with Ki-67 and proliferating cell nuclear antigen (PCNA) as proliferative markers. Different morphological parameters were compared before and after oncotherapy by unpaired Student's t test. Statistically significant differences were found in morphometric parameters, e.g., mean nuclear diameter, mean nuclear area, mean cell diameter, and mean cell area, and in the expression of proliferative markers (Ki-67 and PCNA). Statistical analysis was done by obtaining p values. There are statistically significant differences between morphological parameter of breast carcinoma cells before and after oncotherapy.
authors.affiliation: Department of Pathology, IPGMER, Kolkata, West Bengal India.
authors.forename: Sananda
authors.initials: S
authors.lastname: Koley
authors.affiliation: Department of Pathology, BSMC&H, Bankura, West Bengal India.
authors.forename: Srabani
authors.initials: S
authors.lastname: Chakrabarti
authors.affiliation: Department of Pathology, BSMC&H, Bankura, West Bengal India.
authors.forename: Swapan
authors.initials: S
authors.lastname: Pathak
authors.affiliation: Department of Pathology, IPGMER, Kolkata, West Bengal India ; D-17/14, Karunamoyee Housing Estate, Salt Lake, Kolkata, 700091 West Bengal India.
authors.forename: Asim Kumar
authors.initials: AK
authors.lastname: Manna
authors.affiliation: Department of Radiotherapy, IPGMER, 244 AJC Bose Road, Kolkata, 700020 West Bengal India.
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Basu
keywords: Breast carcinoma; Morphometry; Oncotherapy; Proliferative activity