title: [Emodin Induces Apoptosis of K562/Adr Cells Probably through Akt-Caspase 3 Signal Pathway].
abstract: OBJECTIVE
  To investigate the apoptosis-inducing effects of emodin on multidrug resistant leukemia cell line K562/Adr, and to explore the role of Akt-Caspase 3 signal pathway in apoptosis of K562/Adr cells treated with emodin.
  
  
  METHODS
  K562/Adr cells were exposed to emodin of different doses. The ability of emodin to induce apoptosis of K562/Adr cells was detected by Annexin V/PI double labeled flow cytometry and DNA ploidy analysis, the expressions of procaspase-3, PARP, Akt, p-Akt protein were determined by Western blot.
  
  
  RESULTS
  Apoptosis in K562/Adr cells could be induced by emodin in a dose dependent manner, Western blot results showed that emodin down-regulated the expression levels of procaspase-3, Akt, p-Akt, PARA 116 KD in treated K562/Adr cells, up-regulated expressions leves of PARP 85 KD in a time-dependent manner.
  
  
  CONCLUSIONS
  The Akt-Caspase 3 signal pathway may be involved in these processes.
authors.affiliation: Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China.|Department of Hematology, The First Hospital of Putian City, Putian 351100, Fujian Province, China.
authors.forename: He-Yong
authors.initials: HY
authors.lastname: Zheng
authors.affiliation: Department of Hematology, The First Hospital of Putian City, Putian 351100, Fujian Province, China.
authors.forename: Wu-Qiang
authors.initials: WQ
authors.lastname: Lin
authors.affiliation: Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China. E-mail: jdhu@medmail.com.cn.
authors.forename: Jian-Da
authors.initials: JD
authors.lastname: Hu
authors.affiliation: Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Lin
authors.affiliation: Department of Hematology, The First Hospital of Putian City, Putian 351100, Fujian Province, China.
authors.forename: Lin-Jun
authors.initials: LJ
authors.lastname: Xie
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D015536:Down-Regulation; D004642:Emodin; D006801:Humans; D020014:K562 Cells; D015398:Signal Transduction
chemical_list: C505407:CASP3 protein, human; D053148:Caspase 3; D004642:Emodin