title: Non-contiguous-Finished Genome Sequence and Description of Paenibacillus camerounensis sp. nov.
abstract: Strain G4(T) was isolated from the stool sample of a wild gorilla (Gorilla gorilla gorilla) from Cameroon. It is a facultative anaerobic, Gram-negative, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.48% with Paenibacillus typhae, the phylogenetically closest species with standing nomenclature. Moreover, the strain G4(T) presents some phenotypic differences when compared to other Paenibacillus species and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here, we describe the characteristics of this organism, complete genome sequence, and annotation. The 6,933,847 bp size genome (1 chromosome but no plasmid) contains 5972 protein-coding genes and 54 RNAs genes, including 44 tRNA genes. In addition, digital DNA-DNA hybridization values for the genome of the strain G4(T) against the closest Paenibacillus genomes range between 19.7 and 22.1, once again confirming its new status as a new species. On the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we propose the creation of Paenibacillus camerounensis sp. nov. that contains the strain G4(T).
authors.affiliation: Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France.
authors.forename: Mamadou Bhoye
authors.initials: MB
authors.lastname: Keita
authors.affiliation: Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France.
authors.forename: Roshan
authors.initials: R
authors.lastname: Padhmanabhan
authors.affiliation: Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robert
authors.affiliation: IRD, University Montpellier 1, Montpellier, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Delaporte
authors.affiliation: Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France.|King Fahad Medical Research Center, King Abdul Aziz University, Jeddah, Saudi Arabia.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France.
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
authors.affiliation: Faculté de médecine, URMITE, Aix-Marseille Université, Marseille, France. fadi.bittar@univ-amu.fr.
authors.forename: Fadi
authors.initials: F
authors.lastname: Bittar
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002163:Cameroon; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D020459:Genes, rRNA; D006071:Gorilla gorilla / Q000382:microbiology; D056507:Paenibacillus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer
keywords: Culturomics; Genome; Paenibacillus camerounensis; Taxono-genomics