title: [Successful pregnancy following laser-assisted selection of viable but immotile spermatozoa for intracytoplasmic sperm injection: A report of 2 cases].
abstract: OBJECTIVE
  To investigate the feasibility and clinical application value of selecting viable spermatozoa by noncontact diode laser.
  
  
  METHODS
  We obtained immotile spermatozoa from 2 infertile men with obstructive azoospermia or severe asthenospermia and selected viable spermatozoa using a single laser shot at the sperm tail. Those that responded to the laser shot by a curling reaction of the tail were regarded as presumably viable and used for intracytoplasmic sperm injection (ICSI).
  
  
  RESULTS
  The mean fertilization rate was 88.89% after ICSI with the laser-selected viable spermatozoa. Both of the embryo transfers resulted in a single pregnancy.
  
  
  CONCLUSIONS
  Noncontact diode laser is a useful alternative for the assessment of sperm viability, which may help to achieve successful pregnancy.
authors.forename: Huan-hua
authors.initials: HH
authors.lastname: Chen
authors.forename: Gui-xue
authors.initials: GX
authors.lastname: Feng
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Shu
authors.forename: Xian-you
authors.initials: XY
authors.lastname: Gan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Ruo-yun
authors.initials: RY
authors.lastname: Lin
mesh_terms: D004624:Embryo Transfer; D005260:Female; D005306:Fertilization; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013082:Sperm Tail / Q000502:physiology