title: Identification and expression analysis of a new invertebrate lysozyme in Kuruma shrimp (Marsupenaeus japonicus).
abstract: Lysozyme is an important component of the innate immunity system against invading pathogens. An invertebrate (i-type) lysozyme from the hepatopancreas of Kuruma shrimp Marsupenaeus japonicus (Mj-ilys) was identified. The full-length cDNA of Mj-ilys was 580bp with a 429 bp open reading frame encoding a 142 amino acid polypeptide. The encoded polypeptide was predicted to have a 17 amino acid signal peptide, and a 125 amino acid mature protein with a theoretical mass of 14.099 kDa and an isoelectric point (pI) of 4.18. A Destabilase conserved domain was predicted in Mj-ilys amino acid sequences which may be stable by 10 cysteine residues forming 5 disulfide bonds. Mj-ilys may loss the muramidase and isopeptidase activities due to the lack of the key catalytic residues. Mj-ilys had high homologous of 80-82% with i-type lysozymes of penaeid shrimps. It was first grouped with other i-type lysozyme of shrimps and crabs in a phylogenetic tree predicted by the Neighbor-Joining method. Mj-ilys mRNA was expressed mainly in hepatopancreas and almost undetectable in other tissues. The mRNA expression of Mj-ilys were all found from fertilized eggs to post-larvae of 17 days (PL17), and its expression exhibited significant differences among each developmental stage. After white spot syndrome virus (WSSV) challenge (3.6 × 10(8) virions/μl), the time-dependent expression pattern of Mj-ilys in hepatopancreas and gills showed significantly different. These results indicated that Mj-ilys is potentially involved in the ontogenesis and immune defense in Kuruma shrimp.
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, Fujian 361102, China. Electronic address: yqsu@xmu.edu.cn.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Su
authors.affiliation: Putian Fishery Institute, Putian, Fujian 351100, China.
authors.forename: Chun-Zhong
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Putian Tian-ran-xing Agricultural Development Co. Ltd., Putian, Fujian 351100, China.
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D043143:Hepatopancreas / Q000201:enzymology; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D033561:Penaeidae / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000821:virology; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D009113:Muramidase
keywords: Developmental expression; Immune response; Invertebrate lysozyme; Marsupenaeus japonicus; WSSV