title: Juvenile Hormone Activates the Transcription of Cell-division-cycle 6 (Cdc6) for Polyploidy-dependent Insect Vitellogenesis and Oogenesis.
abstract: Although juvenile hormone (JH) is known to prevent insect larval metamorphosis and stimulate adult reproduction, the molecular mechanisms of JH action in insect reproduction remain largely unknown. Earlier, we reported that the JH-receptor complex, composed of methoprene-tolerant and steroid receptor co-activator, acts on mini-chromosome maintenance (Mcm) genes Mcm4 and Mcm7 to promote DNA replication and polyploidy for the massive vitellogenin (Vg) synthesis required for egg production in the migratory locust (Guo, W., Wu, Z., Song, J., Jiang, F., Wang, Z., Deng, S., Walker, V. K., and Zhou, S. (2014) PLoS Genet. 10, e1004702). In this study we have investigated the involvement of cell-division-cycle 6 (Cdc6) in JH-dependent vitellogenesis and oogenesis, as Cdc6 is essential for the formation of prereplication complex. We demonstrate here that Cdc6 is expressed in response to JH and methoprene-tolerant, and Cdc6 transcription is directly regulated by the JH-receptor complex. Knockdown of Cdc6 inhibits polyploidization of fat body and follicle cells, resulting in the substantial reduction of Vg expression in the fat body as well as severely impaired oocyte maturation and ovarian growth. Our data indicate the involvement of Cdc6 in JH pathway and a pivotal role of Cdc6 in JH-mediated polyploidization, vitellogenesis, and oogenesis.
authors.affiliation: From the School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Zhongxia
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin 30012, China, and.
authors.forename: Yingtian
authors.initials: Y
authors.lastname: Xie
authors.affiliation: State Key laboratory of Cotton Biology, Institute of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China szhou@henu.edu.cn.
authors.forename: Shutang
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005216:Fat Body / Q000378:metabolism; D005260:Female; D006110:Grasshoppers / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D007605:Juvenile Hormones / Q000378:metabolism*; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000378:metabolism; D011123:Polyploidy; D015533:Transcriptional Activation; D047048:Vitellins / Q000235:genetics / Q000378:metabolism; D014818:Vitellogenesis
chemical_list: C504976:Cdc6 protein, Drosophila; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D007605:Juvenile Hormones; D047048:Vitellins
keywords: DNA replication; gene regulation; insect; juvenile hormone (JH); reproduction