title: [Effect of fluoride on gut microflora of silkworm (Bombyx mori)].
abstract: OBJECTIVE
  We examined the effect of fluoride on gut microflora of silkworm.
  
  
  METHODS
  After DNA extraction and PCR amplification, clone libraries of 16S rRNA gene fragment were constructed. Amplified ribosomal DNA restriction analysis (ARDRA) was performed by digestion of the 16S rRNA gene, and each unique restriction fragment polymorphism pattern was designated as an operational taxonomic unit (OTU). A total of 14 OTUs were identified from intestinal samples of both T6 and 734. Phylogenetic trees of bacterial 16S rRNA nucleotide sequences were constructed and analyzed. Furthermore, the dominant bacteria were studied by the nested polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DDGE) technology.
  
  
  RESULTS
  After fluorosis, the flora of Enterococcus and Bacillus reduced. However, the flora of Staphylococcus increased.
  
  
  CONCLUSIONS
  Fluoride can destroy the balance of microflora in the gut of silkworm by changing the bacteria diversity and proportion, which has bigger effect to 734 than T6.
authors.forename: Guannan
authors.initials: G
authors.lastname: Li
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Xia
authors.forename: Parfait
authors.initials: P
authors.lastname: Sendegeya
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhao
authors.forename: Yaohang
authors.initials: Y
authors.lastname: Long
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D012831:Bombyx / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005459:Fluorides / Q000494:pharmacology*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D007422:Intestines / Q000187:drug effects / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005459:Fluorides