title: Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards.
abstract: Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.
authors.affiliation: Laboratoire de Virologie, Hôpitaux Universitaires de Strasbourg, Strasbourg France. Electronic address: heidi.barth@chru-strasbourg.fr.
authors.forename: Heidi
authors.initials: H
authors.lastname: Barth
authors.affiliation: EA 3181, LabEx LipSTIC ANR-11-LABX-0021, CIC-1431, Université de Franche-Comté, Besançon, France. Electronic address: adrien.morel@univ-fcomte.fr.
authors.forename: Adrien
authors.initials: A
authors.lastname: Morel
authors.affiliation: EA 3181, LabEx LipSTIC ANR-11-LABX-0021, CIC-1431, Université de Franche-Comté, Besançon, France. Electronic address: christiane.mougin@univ-fcomte.fr.
authors.forename: Christiane
authors.initials: C
authors.lastname: Mougin
authors.affiliation: Département de Pathologie, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, France. Electronic address: gerlinde.averous@chru-strasbourg.fr.
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Averous
authors.affiliation: Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, France. Electronic address: michele.jacobs@chru-strasbourg.fr.
authors.forename: Michèle
authors.initials: M
authors.lastname: Legrain
authors.affiliation: EVE Association, Graffenstaden, France. Electronic address: Muriel.Fender@association-eve-alsace.fr.
authors.forename: Muriel
authors.initials: M
authors.lastname: Fender
authors.affiliation: Laboratoire de Virologie, Hôpitaux Universitaires de Strasbourg, Strasbourg France. Electronic address: simone.risch@chru-strasbourg.fr.
authors.forename: Simone
authors.initials: S
authors.lastname: Risch
authors.affiliation: Laboratoire de Virologie, Hôpitaux Universitaires de Strasbourg, Strasbourg France. Electronic address: samira.fafi-kremer@chru-strasbourg.fr.
authors.forename: Samira
authors.initials: S
authors.lastname: Fafi-Kremer
authors.affiliation: Laboratoire d'Epidémiologie et de Santé Publique, EA 3430, Faculté de Médecine, Université de Strasbourg, France. Electronic address: michel.velten@unistra.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Velten
authors.affiliation: Cancéropôle du Grand-Est, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, France. Electronic address: pierre.oudet@canceropole-ge.org.
authors.forename: Pierre
authors.initials: P
authors.lastname: Oudet
authors.affiliation: EA 3181, LabEx LipSTIC ANR-11-LABX-0021, CIC-1431, Université de Franche-Comté, Besançon, France; Département de Gynécologie-Obstétrique, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, France. Electronic address: jean-jacques.baldauf@chru-strasbourg.fr.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Baldauf
authors.affiliation: Laboratoire de Virologie, Hôpitaux Universitaires de Strasbourg, Strasbourg France. Electronic address: francoise.stoll@unistra.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Stoll-Keller
mesh_terms: D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology; D003584:Cytological Techniques / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology; D011309:Preservation, Biological / Q000379:methods*; D015203:Reproducibility of Results; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods*; D014773:Virology / Q000379:methods*; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: Biobanking; Cervical cancer; Papillomavirus; Storage of cells