title: Electrochemiluminescence resonance energy transfer between graphene quantum dots and graphene oxide for sensitive protein kinase activity and inhibitor sensing.
abstract: Herein, a novel electrochemiluminescence resonance energy transfer (ECL-RET) biosensor using graphene quantum dots (GQDs) as donor and graphene oxide (GO) as acceptor for monitoring the activity of protein kinase was presented for the first time. Anti-phosphoserine antibody conjugated graphene oxide (Ab-GO) nonocomposite could be captured onto the phosphorylated peptide/GQDs modified electrode surface through antibody-antigen interaction in the presence of casein kinase II (CK2) and adenosine 5'-triphosphate (ATP), resulting in ECL from the GQDs quenching by closely contacting GO. This ECL quenching degree was positively correlated with CK2 activity. Therefore, on the basis of ECL-RET between GQDs and GO, the activity of protein kinase can be detected sensitively. This biosensor can also be used for quantitative analysis CK2 activity in serum samples and qualitative screening kinase inhibition, indicating the potential application of the developed method in biochemical fundamental research and clinical diagnosis.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, PR China.
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Liang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, PR China.
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Qiu
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, PR China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, PR China.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Xiang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, PR China. Electronic address: jdqiu@ncu.edu.cn.
authors.forename: Jian-Ding
authors.initials: JD
authors.lastname: Qiu
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry*; D049449:Luminescence; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D045663:Quantum Dots; D015203:Reproducibility of Results
chemical_list: D047428:Protein Kinase Inhibitors; D006108:Graphite; D011494:Protein Kinases
keywords: Casein kinase II activity; Electrochemiluminescence resonance energy transfer; Graphene oxide; Graphene quantum dots