title: Chiral bioaccumulation behavior of tebuconazole in the zebrafish (Danio rerio).
abstract: Tebuconazole is an effective chiral fungicide, and previous studies have demonstrated that tebuconazole enantiomers exhibit enantioselective toxicity to non-target aquatic organisms. Thus, the aim of the present study was to investigate the chiral bioaccumulation behavior of tebuconazole in zebrafish (Danio rerio). Two exposure concentrations (0.107 and 1.07 mg/L) of tebuconazole were used. The uptake experiments lasted for 8 days, and subsequently, the zebrafish were transferred to another clean tank containing water without tebuconazole for depuration experiments (up to 14 days). A significant trend in enantioselective bioaccumulation was observed in these zebrafish with the preferential accumulation of (-)-R-tebuconazole at two dose levels. The results of the depuration experiments indicated that the degradation of (-)-R-tebuconazole in zebrafish was slower than that of (+)-S-tebuconazole. The BCFk values for (+)-S-tebuconazole and (-)-R-tebuconazole in a low dose of this chemical were 11.22 and 16.25, respectively, while at a high dose, these values were 9.79 and 10.31, respectively. The enantiomer fraction of tebuconazole in zebrafish and water ranged from 0.31-0.49. Hence, future research should focus on the fate of tebuconazole in the aquatic environment at the enantiomer levels.
authors.affiliation: Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, PR China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China.
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China. Electronic address: zhengyongquan@ippcaas.cn.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D005659:Fungicides, Industrial / Q000737:chemistry / Q000493:pharmacokinetics*; D013237:Stereoisomerism; D014230:Triazoles / Q000737:chemistry / Q000493:pharmacokinetics*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000493:pharmacokinetics*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D005659:Fungicides, Industrial; D014230:Triazoles; D014874:Water Pollutants, Chemical; C087114:tebuconazole
keywords: Bioaccumulation; Enantioselectivity; Tebuconazole; Zebrafish