title: [Macrophage phagocytosis enhanced by Tiaohengfang polysaccharides in vitro].
abstract: OBJECTIVE
  To study the immunoregulatory effect of Tiaohengfang polysaccharides (THFPS) on the phagocytosis of macrophages.
  
  
  METHODS
  According to the preparing method of Chinese medicine polysaccharides, crude polysaccharides were extracted from Tiaohengfang. Mouse peritoneal macrophages were separated and cultured. After the macrophages were treated with (500, 200, 10) μg/mL THFPS for 48 hours, the cell morphology was observed under a light microscope. Phagocytic tests of ink and Staphylococcus aureus were used to evaluate the phagocytosis of the macrophages, and cell enzyme chemical staining was applied to observe the changes of acid phosphatase in macrophages.
  
  
  RESULTS
  Compared with the control group, the volume of macrophages significantly became bigger when they were treated with (500, 200, 10) μg/mL THFPS, the intake of ink and Staphylococcus aureus significantly increased, and the activity of acid phosphatase was also significantly enhanced, which was positively correlated with the dose of THFPS.
  
  
  CONCLUSIONS
  THFPS can enhance the phagocytosis of macrophages and increase the activity of intracellular acid phosphatase.
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China. *Corresponding author, E-mail: zhanghf968@163.com.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Penglong
authors.initials: P
authors.lastname: Yu
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Dongbo
authors.initials: D
authors.lastname: Yu
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Xisheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Huan
authors.affiliation: Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000135:Acid Phosphatase / Q000276:immunology / Q000378:metabolism; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000276:immunology
chemical_list: D011134:Polysaccharides; D000135:Acid Phosphatase