title: Activation of SHH signaling pathway promotes vasculogenesis in post-myocardial ischemic-reperfusion injury.
abstract: This study aimed to investigate the potential roles of sonic Hedgehog (SHH) expression in vasculogenesis in post-myocardial ischemic-reperfusion injury (MIRI) and its underlying mechanism. Cardiac microvascular endothelial cells (CMECs) isolated from the SD rat hearts tissues were used to construct the MIRI model. mRNA level of SHH in control cells and MIRI cells was detected using RT-PCR analysis. Furthermore, effects of SHH expression on CMECs viability and apoptosis were analyzed using MTT assay and Annexin-V-FITC kit respectively. Moreover, effects of SHH expression on the pathway signal proteins expression was analyzed using ELISA and western blotting. mRNA level of SHH was significantly decreased compared to the controls (P<0.05). Besides, CMECs viability was significantly increased while cell apoptosis was decreased by SHH application compared with the controls (P<0.05). Vasculogenesis-related factors including VEGF, FGF and Ang were significantly increased by SHH application, as well as the SHH signal proteins including Patch-1, Gli1, Gli2 and SMO (P<0.05). However, these effects of SHH application on biological factors levels were reversed by the SHH inhibitor application. This study suggested that SHH over expression may play a pivotal contribute role in vasculogenesis through activating the SHH signals in post-MIRI.
authors.affiliation: Qingdao University Qingdao 266000, Shandong Province, China ; Department of Geratology, Jining NO. 1 People's Hospital Jining 272011, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Laboratory, Jining NO. 1 People's Hospital Jining 272011, Shandong Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of Cardiology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University Yantai 264000, Shandong Province, China.
authors.forename: Faxin
authors.initials: F
authors.lastname: Ren
authors.affiliation: Department of Geratology, Jining NO. 1 People's Hospital Jining 272011, Shandong Province, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Geratology, Jining NO. 1 People's Hospital Jining 272011, Shandong Province, China.
authors.forename: Suning
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University Yantai 264000, Shandong Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D053823:Hedgehog Proteins / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D053823:Hedgehog Proteins; C546888:Shh protein, rat
keywords: Myocardial ischemic-reperfusion injury; SHH; cell apoptosis; cell viability; vasculogenesis