title: The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
abstract: mCLING is a fixable endocytosis marker that can be combined with immunolabeling techniques to study the molecular composition of trafficking organelles. mCLING can be used both in cultured cells and in tissue if critical sample preparation steps, such as fixation, are correctly performed. This unit describes protocols for the application of mCLING and for the subsequent sample processing. We include immunostaining protocols and embedding procedures for confocal and high-resolution microscopy.
authors.affiliation: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen.|Department of Tumor Immunology, Institute for Molecular Life Sciences, Radboud University Medical Center.
authors.forename: Natalia H
authors.initials: NH
authors.lastname: Revelo
authors.affiliation: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen.
authors.forename: Silvio O
authors.initials: SO
authors.lastname: Rizzoli
mesh_terms: D000818:Animals; D015415:Biomarkers; D002462:Cell Membrane / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D009488:Neurosciences / Q000379:methods*; D015388:Organelles / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology*
chemical_list: D015415:Biomarkers
keywords: endocytosis; high-resolution microscopy; immunolabeling; mCLING; membrane trafficking