title: In vivo pharmacokinetics of and tissue distribution study of physalin B after intravenous administration in rats by liquid chromatography with tandem mass spectrometry.
abstract: A rapid and sensitive liquid chromatography tandem mass spectrometry quantitative analysis method was established for the pharmacokinetics and tissue distribution study of physalin B in rat. Physalin B and physalin H (internal standard, IS) were separated on an Agilent Eclips XDB C8 column. MS detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode with a positive eletrospray ionization source. The assay was validated in the concentration ranges of 22.6-22600 ng/mL for heart and lung and 4.52-4520 ng/mL for other tissues. The intra- and inter-day precisions (RSD) were ≤9.23 and ≤12.51%, respectively, with accuracy (%) in the range of 88.07-113.2%. A pharmacokinetic study showed that physalin B has a long dwell time with a half-life of 321.2 ± 29.5 min and clearance of 175.4 ± 25.7 mL/min/kg after intravenous administration. Additionally, physalin B showed a wide tissue distribution with a special higher penetration in lung. The data presented in this study could provide useful information for the further study of physalin B. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, 79# Qingchun Road, Hangzhou, 310003, People's Republic of China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, People's Republic of China.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, People's Republic of China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Hong
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D007262:Infusions, Intravenous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012632:Secosteroids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D012632:Secosteroids; C033270:physalin B
keywords: LC-MS/MS; pharmacokinetics; physalin B; tissue distribution