title: Genetic polymorphisms of the drug-metabolizing enzyme cytochrome P450 3A5 in a Uyghur Chinese population.
abstract: 1.  Detection of CYP3A5 variant alleles, and knowledge about their allelic frequency in Uyghur ethnic groups, is important to establish the clinical relevance of screening for these polymorphisms to optimize pharmacotherapy. 2. We used DNA sequencing to investigate the promoter, exons and surrounding introns, and 3'-untranslated region of the CYP3A5 gene in 96 unrelated healthy Uyghur individuals. We also used SIFT and PolyPhen-2 to predict the protein function of the novel non-synonymous mutation in CYP3A5 coding regions. 3. We found 24 different CYP3A5 polymorphisms in the Uyghur population, three of which were novel: the synonymous mutation 43C > T in exon 1, two mutations 32120C > G and 32245T > C in 3'-untranslated region, and we detected the allele frequencies of CYP3A5*1 and *3 as 64.58% and 35.42%, respectively. While no subjects with CYP3A5*6 were identified. Other identified genotypes included the heterozygous genotype 1A/3A (59.38%) and 1A/3E (11.46%), which lead to decreased enzyme activity. In addition, the frequency of haplotype "TTAGGT" was the most prevalent with 0.781. 4. Our data provide new information regarding CYP3A5 genetic polymorphisms in Uyghur individuals, which may help to improve individualization of drug therapy and offer a preliminary basis for more rational use of drugs.
authors.affiliation: a School of Life Sciences, Northwest University , Xi'an , China .|b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Zhengshuai
authors.initials: Z
authors.lastname: Chen
authors.affiliation: a School of Life Sciences, Northwest University , Xi'an , China .|b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Jingjie
authors.initials: J
authors.lastname: Li
authors.affiliation: a School of Life Sciences, Northwest University , Xi'an , China .|b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Wang
authors.affiliation: b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: a School of Life Sciences, Northwest University , Xi'an , China .|b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .|c Key Laboratory of High Altitude Environment and Genes Related to Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University , Xianyang , China , and.|d Key Laboratory for Basic Life Science Research of Tibet Autonomous Region, School of Medicine, Xizang Minzu University , Xianyang , China.
authors.forename: Tianbo
authors.initials: T
authors.lastname: Jin
authors.affiliation: a School of Life Sciences, Northwest University , Xi'an , China .|b National Engineering Research Center for Miniaturized Detection Systems , Xi'an , China .
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
mesh_terms: D044466:Asian People; D002681:China; D051544:Cytochrome P-450 CYP3A / Q000235:genetics* / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D020641:Polymorphism, Single Nucleotide
chemical_list: D051544:Cytochrome P-450 CYP3A
keywords: CYP3A5; Uyghur population; genetic polymorphisms; haplotype