title: Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking.
abstract: This study was aimed to evaluate the effect of (124)I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [(124)I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0 Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [(124)I]HIB-labeled ADSCs. We demonstrated that (124)I-labeling with HIB did not affect the biological properties of ADSCs.
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea; School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Min Hwan
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Kyo Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Gwang Il
authors.initials: GI
authors.lastname: An
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Sang-Keun
authors.initials: SK
authors.lastname: Woo
authors.affiliation: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Noh Won
authors.initials: NW
authors.lastname: Park
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Eom
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Tae Sup
authors.initials: TS
authors.lastname: Lee
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Chan Wha
authors.initials: CW
authors.lastname: Kim
authors.affiliation: Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Jeongsoo
authors.initials: J
authors.lastname: Yoo
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea. Electronic address: yjlee@kirams.re.kr.
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000000981:diagnostic imaging / Q000187:drug effects / Q000637:transplantation; D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D007457:Iodine Radioisotopes / Q000009:adverse effects; D007463:Iodobenzoates / Q000009:adverse effects*; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000628:therapy*; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D013234:Stem Cells / Q000000981:diagnostic imaging* / Q000187:drug effects; D016896:Treatment Outcome
chemical_list: D007457:Iodine Radioisotopes; D007463:Iodobenzoates; D019275:Radiopharmaceuticals
keywords: Adipose-derived stem cells; Direct labeling agent; Hexadecyl-4-[(124)I]iodobenzoate; Microarray; Myocardial infarction