title: Motion reduction and multidimensional denoising in Voltage-sensitive Dye imaging.
abstract: Optical Imaging using Voltage-sensitive Dyes is characterized by low fractional changes in fluorescent light intensity upon the application of a stimulus, which leads to slight value differences between pixels on an in-general noisy image sequence. The application of an anisotropic diffusion filtering scheme, in order to contribute to the denoising of the optical images, is proposed as one option to improve its quality and for a better understanding of the physiological processes they represent. We apply an image registration approach to compensate for motion artifacts, such that we do not need to mount a fixed cranial chamber onto the skull. In this work, electrical stimulation to the tibial nerve in a rat model was used to register evoke potentials, imaging the somatosensory cortex of the animal, which was previously stained with the RH1691 dye.
authors.forename: A
authors.initials: A
authors.lastname: Romero
authors.forename: K
authors.initials: K
authors.lastname: Schwerdtfeger
authors.forename: J
authors.initials: J
authors.lastname: Szczygielski
authors.forename: P
authors.initials: P
authors.lastname: Flotho
authors.forename: J K
authors.initials: JK
authors.lastname: Schubert
authors.forename: L
authors.initials: L
authors.lastname: Haab
authors.forename: M
authors.initials: M
authors.lastname: Hmila
authors.forename: D J
authors.initials: DJ
authors.lastname: Strauss
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D004558:Electric Stimulation; D005071:Evoked Potentials; D005260:Female; D008279:Magnetic Resonance Imaging; D008297:Male; D009068:Movement; D051381:Rats; D017207:Rats, Sprague-Dawley; D059629:Signal-To-Noise Ratio; D013003:Somatosensory Cortex / Q000502:physiology; D013979:Tibial Nerve / Q000502:physiology; D056969:Voltage-Sensitive Dye Imaging / Q000379:methods*