title: Design of an implantable artificial dural window for chronic two-photon optical imaging in non-human primates.
abstract: Optical functional imaging methods such as calcium imaging have become a powerful tool for investigating neural activity in-vivo. Here, we present a design for a titanium implantable chamber with transparent silicone artificial dura which enables two-photon calcium imaging in non-human primates. This chamber accommodates imaging with high numerical aperture multiphoton objective lenses, and remains sealed, protecting the brain from the surrounding environment. In addition, we describe a tunable tissue stabilization system to apply gentle mechanical pressure to stabilize tissue during imaging. Our results suggest that two-photon calcium imaging may soon facilitate a new class of circuit and systems neuroscience experiments in non-human primates.
authors.forename: Eric
authors.initials: E
authors.lastname: Trautmann
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: O'Shea
authors.forename: Shikhar
authors.initials: S
authors.lastname: Shrestha
authors.forename: Steven
authors.initials: S
authors.lastname: Lin
authors.forename: Stephen
authors.initials: S
authors.lastname: Ryu
authors.forename: Krishna
authors.initials: K
authors.lastname: Shenoy
mesh_terms: D000818:Animals; D004388:Dura Mater; D020007:Implants, Experimental; D008251:Macaca; D061848:Optical Imaging