title: Cytoskeletal binding proteins distinguish cultured dental follicle cells and periodontal ligament cells.
abstract: Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts and osteoblasts, these cells form cementum, periodontal ligament and alveolar bone, respectively. While stem cells in dental follicle are a precursor to periodontal ligament fibroblasts, the molecular changes that distinguish cultured DFCs from PDLCs are still unknown. In this study, we have compared the immunophenotypic features and cell cycle status of the two cell lines. The results suggest that DFCs and PDLCs displayed similar features related to immunophenotype and cell cycle. Then we employed an isobaric tag for relative and absolute quantitation (iTRAQ) proteomics strategy to reveal the molecular differences between the two cell types. A total of 2138 proteins were identified and 39 of these proteins were consistently differentially expressed between DFCs and PDLCs. Gene ontology analyses revealed that the protein subsets expressed higher in PDLCs were related to actin binding, cytoskeletal protein binding, and structural constituent of muscle. Upon validation by real-time PCR, western blotting, and immunofluorescence staining. Tropomyosin 1 (TPM1) and caldesmon 1 (CALD1) were expressed higher in PDLCs than in DFCs. Our results suggested that PDLCs display enhanced actin cytoskeletal dynamics relative to DFCs while DFCs may exhibit a more robust antioxidant defense ability relative to PDLCs. This study expands our knowledge of the cultured DFCs and PDLCs proteome and provides new insights into possible mechanisms responsible for the different biological features observed in each cell type.
authors.affiliation: College of Life Science, Sichuan University, Chengdu, China; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yang
authors.affiliation: National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Chen
authors.affiliation: National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of Pedodontics, West China School of Stomatology, Sichuan University, Chengdu, China. Electronic address: guoweihua943019@163.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Guo
authors.affiliation: National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China. Electronic address: drtwd@sina.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Tian
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003795:Dental Sac / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D063990:Gene Ontology; D006801:Humans; D016130:Immunophenotyping; D007553:Isotope Labeling; D010012:Osteogenesis; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010513:Periodontal Ligament / Q000166:cytology*; D016133:Polymerase Chain Reaction; D025941:Protein Interaction Mapping; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D000975:Antioxidants; D003598:Cytoskeletal Proteins; D020543:Proteome
keywords: Cytoskeleton; Periodontium; Proteome; Stem cells; Tissue regeneration