title: Inhibition of A2780 Human Ovarian Carcinoma Cell Proliferation by a Rubus Component, Sanguiin H-6.
abstract: The effects of a red raspberry component, sanguiin H-6 (SH-6), on the induction of apoptosis and the related signaling pathways in A2780 human ovarian carcinoma cells were investigated. SH-6 caused an antiproliferative effect and a severe morphological change resembling that of apoptotic cell death but no effect on the cancer cell cycle arrest. In addition, SH-6 induced an early apoptotic effect and activation of caspases as well as the cleavage of PARP, which is a hallmark of apoptosis. The early apoptotic percentages of A2780 cells exposed to 20 and 40 μM SH-6 were 35.39 and 41.76, respectively. Also, SH-6 caused the activation of mitogen-activated protein kinases (MAPKs), especially p38, and the increase of truncated p15/BID. These results in the present study suggest that the apoptosis of A2780 human ovarian carcinoma cells by SH-6 is mediated by the MAPK p38 and a caspase-8-dependent BID cleavage pathway.
authors.affiliation: College of Korean Medicine, Gachon University , Seongnam 13120, Korea.|Department of Food Science, Gyeongnam National University of Science and Technology , Jinju 660-758, Korea.
authors.forename: Dahae
authors.initials: D
authors.lastname: Lee
authors.affiliation: Laboratory of Molecular Oncology, Cheil General Hospital Women's Healthcare Center, Dankook University College of Medicine , Seoul 04619, Korea.
authors.forename: Hyeonseok
authors.initials: H
authors.lastname: Ko
authors.affiliation: Natural Medicine Center, Korea Institute of Science and Technology , Gangneung 25451, Gangwon-do, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Natural Medicine Center, Korea Institute of Science and Technology , Gangneung 25451, Gangwon-do, Korea.
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Department of Pharmacology, and Cell Dysfunction Research Center (CDRC), University of Ulsan College of Medicine , Seoul 05505, Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Choi
authors.affiliation: College of Korean Medicine, Gachon University , Seongnam 13120, Korea.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamabe
authors.affiliation: Natural Product Research Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 440-746, Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Gachon University , Seongnam 13120, Korea.
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.affiliation: Department of Food Science, Gyeongnam National University of Science and Technology , Jinju 660-758, Korea.
authors.forename: Hyun Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Environmental Toxicology, University of California , Davis, California 95616, United States.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shibamoto
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D065856:Rubus / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006131:Growth Inhibitors; D047348:Hydrolyzable Tannins; D019253:Proto-Oncogene Proteins c-bcl-2; C081844:sanguiin H 6; D053148:Caspase 3; D053181:Caspase 8
keywords: A2780 ovarian carcinoma cells; MAPK p38; apoptosis; caspase; polyphenols; raspberry; sanguiin H-6; truncated BID