title: Sex and Urbanicity Contribute to Variation in Lymphocyte Distribution across Ugandan Populations.
abstract: Management of patient care and interpretation of research data require evaluation of laboratory results in the context of reference data from populations with known health status to adequately diagnose disease or make a physiological assessment. Few studies have addressed the diversity of lymphocyte subsets in rural and urban Ugandan populations. Here, 663 healthy blood bank donors from semi-urban centers of Kampala consented to participate in a study to define lymphocyte reference ranges. Whole blood immunophenotyping was performed to determine the frequency and absolute counts of T, B, and NK cells using clinical flow cytometry. Results from blood bank donors were compared to a rural cohort from the district of Kayunga and more urban clinical trial participants from the capital city, Kampala. Relationships between hematological and lymphocyte parameters were also explored. In the semi-urban blood donors, females were significantly different from males in all parameters except the frequency of CD8 T and B cells. Females had higher absolute counts of CD4 T, CD8 T and B cells, whereas males had higher NK cell counts. NK cell frequency and counts were significantly higher in semi-urban blood donors, regardless of sex, compared to more urban study participants. CD8 T cell frequency and counts were significantly higher in the blood donors compared to the rural participants, irrespective of sex. Interestingly, basophil counts were positively associated with overall T cell counts. These findings suggest that both sex and level of cohort urbanicity may influence lymphocyte subset distributions in Ugandans.
authors.affiliation: Makerere University Walter Reed Project, Kampala, Uganda.|Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
authors.forename: Prossy
authors.initials: P
authors.lastname: Naluyima
authors.affiliation: U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.|Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, United States of America.
authors.forename: Leigh Anne
authors.initials: LA
authors.lastname: Eller
authors.affiliation: Makerere University Walter Reed Project, Kampala, Uganda.
authors.forename: Benson J
authors.initials: BJ
authors.lastname: Ouma
authors.affiliation: Makerere University Walter Reed Project, Kampala, Uganda.
authors.forename: Denis
authors.initials: D
authors.lastname: Kyabaggu
authors.affiliation: Uganda National Blood Transfusion Service, Kampala, Uganda.
authors.forename: Peter
authors.initials: P
authors.lastname: Kataaha
authors.affiliation: School of Public Health, Makerere University College of Health Sciences, Kampala, Uganda.
authors.forename: David
authors.initials: D
authors.lastname: Guwatudde
authors.affiliation: Makerere University Walter Reed Project, Kampala, Uganda.
authors.forename: Hannah
authors.initials: H
authors.lastname: Kibuuka
authors.affiliation: Makerere University Walter Reed Project, Kampala, Uganda.|School of Public Health, Makerere University College of Health Sciences, Kampala, Uganda.
authors.forename: Fred
authors.initials: F
authors.lastname: Wabwire-Mangen
authors.affiliation: U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.|Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, United States of America.
authors.forename: Merlin L
authors.initials: ML
authors.lastname: Robb
authors.affiliation: U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.
authors.forename: Nelson L
authors.initials: NL
authors.lastname: Michael
authors.affiliation: SEARCH, Thai Red Cross AIDS Research Center, Bangkok, Thailand.
authors.forename: Mark S
authors.initials: MS
authors.lastname: de Souza
authors.affiliation: Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
authors.forename: Johan K
authors.initials: JK
authors.lastname: Sandberg
authors.affiliation: U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.|Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, United States of America.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Eller
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001491:Basophils / Q000166:cytology / Q000276:immunology; D001782:Blood Donors / Q000706:statistics & numerical data*; D015331:Cohort Studies; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012424:Rural Population / Q000706:statistics & numerical data*; D012737:Sex Factors; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014454:Uganda; D014505:Urban Population / Q000706:statistics & numerical data*; D055815:Young Adult