title: Study of ion suppression for phenolic compounds in medicinal plant extracts using liquid chromatography-electrospray tandem mass spectrometry.
abstract: A systematic study on the various sources of ion suppression in UHPLC-MS-MS analysis was carried out for 24 phenolic antioxidants in 6 different extracts of medicinal plants from Amazonia. The contributions of matrix effects, mobile-phase additives, analyte co-elution and electric charge competition during ionization to the global ion suppression were evaluated. Herein, the influence of mobile-phase additives on the ionization efficiency was found to be very pronounced, where ion suppression of approximately 90% and ion enhancement effects greater than 400% could be observed. The negative effect caused by the wrong choice of internal standard (IS) on quantitative studies was also evaluated and discussed from the perspective of ion suppression. This work also shows the importance of performing studies with this approach even for very similar matrices, such as varieties of medicinal plants from the same species, because different effects were observed for the same analyte.
authors.affiliation: Graduate Program in Chemistry, Center of Natural Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Faccin
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Viana
authors.affiliation: Graduate Program in Chemistry, Center of Natural Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: P C
authors.initials: PC
authors.lastname: do Nascimento
authors.affiliation: Graduate Program in Chemistry, Center of Natural Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Bohrer
authors.affiliation: Graduate Program in Chemistry, Center of Natural Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil; Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil. Electronic address: leandrocarvalho@pq.cnpq.br.
authors.forename: L M
authors.initials: LM
authors.lastname: de Carvalho
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001938:Brazil; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis; D003374:Coumarins / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013267:Stilbenes / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D003374:Coumarins; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D013267:Stilbenes; D002392:Catechin
keywords: Ion enhancement; Ion suppression; Phenolic antioxidants; Plant extracts; UHPLC-ESI–MS/MS