title: Potent Antibacterial Nanoparticles against Biofilm and Intracellular Bacteria.
abstract: The chronic infections related to biofilm and intracellular bacteria are always hard to be cured because of their inherent resistance to both antimicrobial agents and host defenses. Herein we develop a facile approach to overcome the above conundrum through phosphatidylcholine-decorated Au nanoparticles loaded with gentamicin (GPA NPs). The nanoparticles were characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS) and ultraviolet-visible (UV-vis) absorption spectra which demonstrated that GPA NPs with a diameter of approximately 180 nm were uniform. The loading manner and release behaviors were also investigated. The generated GPA NPs maintained their antibiotic activities against planktonic bacteria, but more effective to damage established biofilms and inhibited biofilm formation of pathogens including Gram-positive and Gram-negative bacteria. In addition, GPA NPs were observed to be nontoxic to RAW 264.7 cells and readily engulfed by the macrophages, which facilitated the killing of intracellular bacteria in infected macrophages. These results suggested GPA NPs might be a promising antibacterial agent for effective treatment of chronic infections due to microbial biofilm and intracellular bacteria.
authors.affiliation: College of science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Mu
authors.affiliation: College of science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Jiangjiang
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Qianjin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Duan
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D002460:Cell Line; D006046:Gold / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D006046:Gold