title: Controlling fungal biofilms with functional drug delivery denture biomaterials.
abstract: Candida-associated denture stomatitis (CADS), caused by colonization and biofilm-formation of Candida species on denture surfaces, is a significant clinical concern. We show here that modification of conventional denture materials with functional groups can significantly increase drug binding capacity and control drug release rate of the resulting denture materials for potentially managing CADS. In our approach, poly(methyl methacrylate) (PMMA)-based denture resins were surface grafted with three kinds of polymers, poly(1-vinyl-2-pyrrolidinone) (PNVP), poly(methacrylic acid) (PMAA), and poly(2-hydroxyethyl methacrylate) (PHEMA), through plasma-initiated grafting polymerization. With a grafting yield as low as 2 wt%, the three classes of new functionalized denture materials showed significantly higher drug binding capacities toward miconazole, a widely used antifungal drug, than the original PMMA denture resin control, leading to sustained drug release and potent biofilm-controlling effects against Candida. Among the three classes of functionalized denture materials, PNVP-grafted resin provided the highest miconazole binding capability and the most powerful antifungal and biofilm-controlling activities. Drug binding mechanisms were studied. These results demonstrated the importance of specific interactions between drug molecules and functional groups on biomaterials, shedding lights on future design of CADS-managing denture materials and other related devices for controlled drug delivery.
authors.affiliation: Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA.
authors.forename: Jianchuan
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA.
authors.forename: Fuguang
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Geriatric Research, Education and Clinical Center, Audie L. Murphy Division, South Texas Veterans Health Care System, San Antonio, TX 78229, USA; Department of Comprehensive Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
authors.forename: Chih-Ko
authors.initials: CK
authors.lastname: Yeh
authors.affiliation: Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA. Electronic address: yuyu_sun@uml.edu.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D002180:Candidiasis, Oral / Q000188:drug therapy / Q000382:microbiology; D003764:Dental Materials / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D008825:Miconazole / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011102:Polyhydroxyethyl Methacrylate / Q000378:metabolism; D011109:Polymethacrylic Acids / Q000378:metabolism; D019904:Polymethyl Methacrylate / Q000378:metabolism; D011145:Polyvinyls / Q000378:metabolism; D011760:Pyrrolidinones / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013282:Stomatitis, Denture / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D003764:Dental Materials; D011109:Polymethacrylic Acids; D011145:Polyvinyls; D011760:Pyrrolidinones; C099680:poly(1-vinyl-2-pyrrolidinone); C030613:polymethacrylic acid; D011102:Polyhydroxyethyl Methacrylate; D008825:Miconazole; D019904:Polymethyl Methacrylate
keywords: Biofilm-controlling; Denture; Drug delivery; Fungal; Grafting