title: The roles of sepsis-induced myeloid derived suppressor cells in mice corneal, skin and combined transplantation.
abstract: OBJECTIVE
  To explore the effects of adoptive transferring sepsis induced myeloid-derived suppressor cells (iMDSCs) in mice corneal, skin, and combined corneal-skin survival.
  
  
  METHODS
  Allogeneic full-thickness corneal transplantation, fully mismatched skin transplantation, and corneal-skin combined transplantation (donor C57BL/6 to recipient Balb/c mice) were performed. Sepsis-induced infectious-MDSCs (iMDSCs), were purified from bone marrow of cecal ligated and punctured (CLP) Balb/c mice. Recipient-derived iMDSCs were adoptively transferred into different recipient groups by retro-orbital injection after surgeries. Corneal and skin grafts were examined and photographed routinely for a period of 45days. Histopathology was performed to evaluate corneal-graft inflammation. Bone marrow and/or corneal grafts in each group were harvested from executed recipients on postoperative days 15, 25, 35. Corneal cells and bone marrow cells were stained with CD11b-PE and Gr1-FITC, analyzed by FACS.
  
  
  RESULTS
  iMDSCs were able to significantly prolong allograft survival in both corneal and corneal-skin combined transplant groups. A substantial expansion of MDSCs was observed in recipients' bone marrow, particularly in combined groups at an early stage postoperatively, and accordingly the concentration of MDSCs in corneal grafts increased significantly in adoptive transferred groups.
  
  
  CONCLUSIONS
  Sepsis-induced MDSCs may suggest a novel cellular therapeutic approach for preventing various types of allograft rejection.
authors.affiliation: Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Eye Institute of The Second Xiangya Hospital of Central South University, Changsha 410010, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China; Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Bei
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China; Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Beijing TongRen Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmic and Visual Science Key Laboratory, Beijing 100730, China. Electronic address: panyj0526@sina.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Pan
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology*; D016039:Corneal Transplantation; D005260:Female; D006085:Graft Survival / Q000276:immunology*; D007165:Immunosuppression Therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology* / Q000382:microbiology / Q000637:transplantation; D018805:Sepsis / Q000276:immunology*; D016038:Skin Transplantation; D063986:Vascularized Composite Allotransplantation
keywords: Adoptive transfer; Allograft rejection; Corneal–skin combined transplantation; MDSC