title: EP3, Prostaglandin E2 Receptor Subtype 3, Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage.
abstract: EP3 is prostaglandin E2 receptor subtype 3 and mediates the activation of several signaling pathways, changing in cAMP levels, calcium mobilization, and activation of phospholipase C. Previous studies demonstrated a direct role for EP3 in various neurodegenerative disorders, such as stroke and Alzheimer disease. However, the distribution and function of EP3 in ICH diseases remain unknown. Here, we demonstrate that EP3 may be involved in neuronal apoptosis in the processes of intracerebral hemorrhage (ICH). From the results of Western blot and immunohistochemistry, we obtained a significant up-regulation of EP3 in neurons adjacent to the hematoma following ICH. Up-regulation of EP3 was found to be accompanied by the increased expression of active caspase-3 and pro-apoptotic Bcl-2-associated X protein (Bax) and decreased expression of anti-apoptotic protein B cell lymphoma-2 (Bcl-2) in vivo and vitro studies. Furthermore, the expression of these three proteins reduced active caspase-3 and Bax expression, while increased Bcl-2 were changed after knocking down EP3 by RNA interference in PC12 cells, further confirmed that EP3 might exert its pro-apoptotic function on neuronal apoptosis. Thus, EP3 may play a role in promoting the neuronal apoptosis following ICH.
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Haidan
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Jiabing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Maohong
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Ning
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. kekaifu_nt@126.com.
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D006406:Hematoma / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D058308:Receptors, Prostaglandin E, EP3 Subtype / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D058308:Receptors, Prostaglandin E, EP3 Subtype; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Apoptosis; EP3; Intracerebral hemorrhage; Neuron; Rat