title: Construction of an Efficient Mutant Strain of Trichosporonoides oedocephalis with HOG1 Gene Deletion for Production of Erythritol.
abstract: The mitogen-activated protein kinase HOG1 (high-osmolarity glycerol response pathway) plays a crucial role in the response of yeast to hyperosmotic shock. Trichosporonoides oedocephalis produces large amounts of polyols (,e.g., erythritol and glycerol) in a culture medium. However, the effects of HOG1 gene knockout and environmental stress on the production of these polyols have not yet been studied. In this study, a To-HOG1 null mutation was constructed in T. oedocephalis using the loxP-Kan-loxP/Cre system as replacement of the targeted genes, and the resultant mutants showed much smaller colonies than the wild-type controls. Interestingly, compared with the wild-type strains, the results of shake-flask culture showed that To-HOG1 null mutation increased erythritol production by 1.44-fold while decreasing glycerol production by 71.23%. In addition, this study investigated the effects of citric acid stress on the T. oedocephalis HOG1 null mutants and the wild-type strain. When the supplementation of citric acid in the fermentation medium was controlled at 0.3% (w/v), the concentration of erythritol produced from the wild-type and To-HOG1 knockout mutant strains improved by 18.21% and 21.65%, respectively.
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Liangzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Guo
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ju
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Hu
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Tang
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Fu
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Gu
authors.affiliation: School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019343:Citric Acid / Q000494:pharmacology; D004896:Erythritol / Q000096:biosynthesis*; D005285:Fermentation; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D005990:Glycerol / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D013312:Stress, Physiological / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D019343:Citric Acid; D020928:Mitogen-Activated Protein Kinases; D005990:Glycerol; D004896:Erythritol
keywords: Erythritol; Gene knockout; HOG pathway; Polyols; Trichosporonoides oedocephalis