title: Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray.
abstract: Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. The abnormal expression of lncRNAs has been implicated in a range of many human diseases, including cancer. But only a small number of functional lncRNAs have been well characterized to date. lncRNA expression profiling may help to identify useful molecular biomarkers and targets for novel therapeutic approaches in the future. In this chapter, we describe a highly efficient lncRNA expression profiling method using a custom-designed microarray.
authors.affiliation: Center for RNA Interference and Non-coding RNAs, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA. xzhang12@mdanderson.org.|Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. xzhang12@mdanderson.org.
authors.forename: Xinna
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Center for RNA Interference andNon-coding RNAs, The University of Texas MD Anderson Cancer Center (MDACC), 1515 Holcombe Blvd., Houston, TX, 77030, USA.|Department of Cancer Biology, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.|Department of Cancer Biology, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Lopez-Berestein
authors.affiliation: Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center (MDACC), 1515 Holcombe Blvd., Houston, TX, 77030, USA.|Center for RNA Interference and Non-Coding RNAs, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.|Department of Cancer Biology, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sood
authors.affiliation: Center for RNA Interference and Non-Coding RNAs, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.|Department of GynecologicOncology and ReproductiveMedicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA.|Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: George A
authors.initials: GA
authors.lastname: Calin
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000295:instrumentation*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D026723:Polyadenylation; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D062085:RNA, Long Noncoding
keywords: Microarray profiling; cDNA labeling; cDNA synthesis; lncRNA expression