title: Differential expression of alkaline phosphatase gene in proliferating primary lymphocytes and malignant lymphoid cell lines.
abstract: Alkaline Phosphatase (APase) activity has been shown to be enhanced specifically in mitogen stimulated B lymphocytes committed to proliferation, but not in T lymphocytes. APase gene expression was analyzed in proliferating murine and human primary lymphocytes and human malignant cell lines using reverse transcriptase and real time PCR. In mitogen stimulated murine splenic lymphocytes, enhancement of APase activity correlated well with an increase in APase gene expression. However, in mitogen stimulated murine T lymphocytes and human PBL despite a vigorous proliferative response, no increase in APase enzyme activity or gene expression was observed. A constitutive expression of APase activity concomitant with APase gene expression was observed inhuman myeloma cell line, U266 B1. However, neither enzyme activity nor gene expression of APase were observed in human T cell lymphoma, SUPT-1. The results suggest a differential expression of APase activity and its gene in proliferating primary lymphocytes of mice and humans. The specific expression of APase activity and its gene only in human myeloma cells, but not in proliferating primary B cells can be exploited as a sensitive disease marker.
authors.affiliation: Cellular Immunology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, TS 500046, India.
authors.forename: S A A
authors.initials: SA
authors.lastname: Latheef
authors.affiliation: Cellular Immunology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, TS 500046, India.
authors.forename: Mallaiah
authors.initials: M
authors.lastname: Devanabanda
authors.affiliation: Cellular Immunology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, TS 500046, India.
authors.forename: Swetha
authors.initials: S
authors.lastname: Sankati
authors.affiliation: Cellular Immunology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, TS 500046, India. Electronic address: mrnsl@uohyd.ernet.in.
authors.forename: Ramanadham
authors.initials: R
authors.lastname: Madduri
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008223:Lymphoma / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase activity; Alkaline phosphatase gene expression; Human peripheral blood lymphocytes; Lipopolysaccharide; Myeloma cell line