title: Adiponectin Induces Oncostatin M Expression in Osteoblasts through the PI3K/Akt Signaling Pathway.
abstract: Rheumatoid arthritis (RA), a common autoimmune disorder, is associated with a chronic inflammatory response and unbalanced bone metabolism within the articular microenvironment. Adiponectin, an adipokine secreted by adipocytes, is involved in multiple functions, including lipid metabolism and pro-inflammatory activity. However, the mechanism of adiponectin performance within arthritic inflammation remains unclear. In this study, we observed the effect of adiponectin on the expression of oncostatin M (OSM), a pro-inflammatory cytokine, in human osteoblastic cells. Pretreatment of cells with inhibitors of phosphatidylinositol 3-kinase (PI3K), Akt, and nuclear factor (NF)-κB reduced the adiponectin-induced OSM expression in osteoblasts. Stimulation of the cells with adiponectin increased phosphorylation of PI3K, Akt, and p65. Adiponectin treatment of osteoblasts increased OSM-luciferase activity and p65 binding to NF-κB on the OSM promoter. Our results indicate that adiponectin increased OSM expression via the PI3K, Akt, and NF-κB signaling pathways in osteoblastic cells, suggesting that adiponectin is a novel target for arthritis treatment.
authors.affiliation: Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang 322100, China. ericsucm@163.com.
authors.forename: Chen-Ming
authors.initials: CM
authors.lastname: Su
authors.affiliation: School of Pharmacy, China Medical University, Taichung 40402, Taiwan. b1204999@hotmail.com.
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Lee
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. jeffrey5991@gmail.com.|Department of Orthopedic Surgery, China Medical University Hospital, Taichung 40402, Taiwan. jeffrey5991@gmail.com.
authors.forename: Chin-Jung
authors.initials: CJ
authors.lastname: Hsu
authors.affiliation: Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang 322100, China. semo1988@163.com.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Lu
authors.affiliation: Department of Orthopedics, Dongyang Peoples' Hospital, Dongyang 322100, China. hustrose666@163.com.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Dongyang Peoples' Hospital, Dongyang 322100, China. dyxghd15@163.com.
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Xu
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. chtang@mail.cmu.edu.tw.|Department of Pharmacology, School of Medicine, China Medical University, Taichung 40402, Taiwan. chtang@mail.cmu.edu.tw.|Department of Biotechnology, College of Health Science, Asia University, Taichung 41354, Taiwan. chtang@mail.cmu.edu.tw.
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Tang
mesh_terms: D052242:Adiponectin / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D053683:Oncostatin M / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015290:Second Messenger Systems
chemical_list: D052242:Adiponectin; D016328:NF-kappa B; D053683:Oncostatin M; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: adiponectin; oncostatin M; osteoblasts; rheumatoid arthritis