title: The role of OsMSH4 in male and female gamete development in rice meiosis.
abstract: Meiosis is essential for gametogenesis in sexual reproduction in rice (Oryza sativa L.). We identified a MutS-homolog (MSH) family gene OsMSH4 in a trisomic plant. Cytological analysis showed that developments of both pollen and embryo sacs in an Osmsh4 mutant were blocked due to defective chromosome pairing. Compared with the wild type, the Osmsh4 mutant displayed a significant ~21.9% reduction in chiasma frequency, which followed a Poisson distribution, suggesting that class I crossover formation in the mutant was impaired. Temporal and spatial expression pattern analyses showed that OsMSH4 was preferentially expressed in meiocytes during their meiosis, indicating a critical role in gametogenesis. Subcellular localization showed that OsMSH4-green fluorescent protein was predominantly located in the nucleus. OsMSH4 could interact with another MSH member (OsMSH5) through the N-terminus and C-terminus, respectively. Direct physical interaction between OsMSH5, OsRPA1a, OsRPA2b, OsRPA1c, and OsRPA2c was identified by yeast two-hybrid assays and further validated by pull-down assays. Our results supported the conclusion that the OsMSH4/5 heterodimer plays a key role in regulation of crossover formation during rice meiosis by interaction with the RPA complex.
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Chaolong
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Peike
authors.initials: P
authors.lastname: Sheng
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Ma
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Erchao
authors.initials: E
authors.lastname: Duan
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Fuqing
authors.initials: F
authors.lastname: Wu
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Linglong
authors.initials: L
authors.lastname: Liu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Qin
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Guo
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Jiulin
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China wanjm@njau.edu.cn wanjm@caas.net.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
mesh_terms: D023902:Chromosome Pairing; D032461:Chromosomes, Plant / Q000378:metabolism; D056130:Gametogenesis, Plant; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008540:Meiosis; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000166:cytology* / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D056771:Ovule / Q000378:metabolism* / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D055503:Protein Multimerization; D021381:Protein Transport; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010940:Plant Proteins
keywords: Chiasmata; Oryza sativa; OsMSH4/OsMSH5 heterodimer; RPA complex; Trisomic.