title: Bioflocculation of the oceanic microalga Dunaliella salina by the bloom-forming dinoflagellate Heterocapsa circularisquama, and its effect on biodiesel properties of the biomass.
abstract: The aim of this work was to evaluate the flocculation by the dinoflagellate Heterocapsa circularisquama as a means for harvesting three Chlorophyta species, Chlorella vulgaris, Nannochloropsis granulata, and Dunaliella salina. Relative fluorescence of D. salina culture significantly decreased along with 9.3-fold increased flocculation activity within 24 h when mixed with H. circularisquama. Lipid content of bioflocculated D. salina increased about 40%, while fatty acid methyl ester (FAME) profiles exhibited higher levels of C16:0, C18:0, and C18:1, compared to harvest by centrifugation, suggesting higher energy content. Furthermore, bioflocculated D. salina biomass had more suitable biodiesel properties relative to both EN14214 and ASTMD6751, with a cetane number of 49.0 and an iodine value of 95.9. These results suggest that H. circularisquama-induced bioflocculation is applicable for the sustainable and qualitative production of algal biodiesel.
authors.affiliation: Korea University of Science & Technology, Daejeon 305-350, Republic of Korea; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
authors.forename: Kichul
authors.initials: K
authors.lastname: Cho
authors.affiliation: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
authors.forename: Sung-Pyo
authors.initials: SP
authors.lastname: Hur
authors.affiliation: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Chi-Heon
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Kyungjun
authors.initials: K
authors.lastname: Ko
authors.affiliation: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Yeon-Ji
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.affiliation: Dongseo Unversity & Technische Universität Berlin Joint Algae Laboratory, Dongseo University, Busan 617-716, Republic of Korea.
authors.forename: Moo-Sang
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Drug & Disease Target Research Team, Korea Basic Science Institute, Daejeon 350-333, Republic of Korea.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Chung
authors.affiliation: Korea University of Science & Technology, Daejeon 305-350, Republic of Korea; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea. Electronic address: dkim@kbsi.re.kr.
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Graduate School of Fisheries Science & Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Oda
mesh_terms: D017756:Aquaculture / Q000379:methods; D056804:Biofuels; D018533:Biomass; D048408:Chlorella vulgaris / Q000378:metabolism; D000460:Chlorophyta / Q000378:metabolism*; D004141:Dinoflagellida / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005427:Flocculation; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism; D009792:Oceans and Seas
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids
keywords: Biodiesel; Dunaliella; Flocculation; Heterocapsa; Microalgae