title: Hispolon inhibits breast cancer cell migration by reversal of epithelial-to-mesenchymal transition via suppressing the ROS/ERK/Slug/E-cadherin pathway.
abstract: Hispolon has been shown to have anticancer effects on various tumors. However, whether hispolon exerts anti-migration activity in breast cancer cells and the underlying mechanisms, have not been elucidated yet. In the present study, our data demonstrated that hispolon inhibited TPA-induced breast cancer MCF-7 cell migration at sub-toxic concentrations in vitro. Hispolon decreased the level of cellular ROS significantly and repressed TPA-induced phosphorylation of extracellular signal-regulated kinase (ERK), accompanied by upregulation of E-cadherin and downregulation of the transcriptional repressor Slug. Furthermore, N-acetyl-cysteine, an antioxidant agent, markedly suppressed TPA-induced epithelial-to-mesenchymal transition, cell migration and activation of ERK. Taken together, our results indicated that hispolon suppressed the migration of breast cancer cells via suppressing the ROS/ERK/Slug/E‑cadherin pathway. Hispolon may be developed as a potential antimetastasis agent to breast cancer.
authors.affiliation: Centre of Laboratory Medicine, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, P.R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, Zhejiang, P.R. China.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Sun
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, Zhejiang, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, Zhejiang, P.R. China.
authors.forename: Long-Xian
authors.initials: LX
authors.lastname: Lv
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, Zhejiang, P.R. China.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology; D061986:MCF-7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D015820:Cadherins; D002396:Catechols; D017382:Reactive Oxygen Species; C103442:hispolon