title: Silencing of ZNF139-siRNA induces apoptosis in human gastric cancer cell line BGC823.
abstract: OBJECTIVE
  ZNF139, a member of zinc finger protein family, is a transcription factor. Our previous studies have showed that the over-expression of ZNF139 in gastric cancer (GC) cells was related to the differentiation of GC. However, the function of ZNF139 in GC cells' apoptosis is still unclear. In present study, endogenous ZNF139 in GC cell line BGC823 was inhibited with siRNA, and then mechanism of ZNF139 in GC cells' apoptosis was investigated.
  
  
  METHODS
  Expression of ZNF139 in GC tissues, adjacent normal tissues, GC cell lines MKN28, SGC7901, BGC823 and that in gastric epithelial cell line GES-1 were tested. Then ZNF139-specific siRNA was transfected into BGC823 cells. Viability, cell cycle and apoptosis of GC cells were detected. Survivin, x-IAP, caspase-3, Fas, p53, Bcl-2 and Bax genes were detected with QPCR and Western blot.
  
  
  RESULTS
  ZNF139 expression in GC tissues was significantly higher than that in adjacent normal tissues; ZNF139 expression in GES-1 was very weak, but it expressed in various GC cell lines, with the highest expression in BGC823. After endogenous ZNF139 was inhibited with ZNF139-siRNA, FCM indicated that after transfection, GC cells in G0/G1 phase was significantly increased, but was significantly reduced in G2/M phases; also after transfection, the apoptotic rate of BGC823 cells increased significantly. 48 h after ZNF139-siRNA was transfected, the expression of Survivin, x-IAP and Bcl-2 was significantly down-regulated, while the expression of caspase-3 and Bax was significantly up-regulated.
  
  
  CONCLUSIONS
  Our results suggest that ZNF139 functions to promote apoptosis resistance of BGC823 by regulating some apoptosis related genes.
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Liqiao
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Bibo
authors.initials: B
authors.lastname: Tan
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Yü
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Fourth Hospital Hebei Medical University Shijiazhuang, China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection
chemical_list: D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; C000590232:ZKSCAN1 protein, human
keywords: Gastric cancer; RNA interference; cell lines; zinc finger protein139