title: Current approaches and challenges for chemical characterization of inhibitory effect against cancer cell line isolated from Gokshur extract.
abstract: In the present study, the potential effect anti tumor and the chemical composition of different fractions of Gokshur was evaluated. Commonly known as puncture vine, it has been used for a long time in both the Indian and traditional Chinese medicine. It is popularly used as a remedy for fertility disorder in Ayurveda. Samples were collected during June-September 2014 in the Cap Bon (north east of the northern Tunisia). Different organs were separated and extracted by sequential process to compare and investigate their potential anti-tumor effect. For the first time, we report the antiproliferatif effect of leaves n-butannolic fraction against cancer cell lines. The better anti-tumor fraction (94.76±1.52%) has been detected and performed by RP-HPLC has shown a great peak area (5578.21Mau). Novel designed natural derivatives from Gokshur, a cyclotrisiloxane, major compound identified by GC-MS.
authors.affiliation: Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta, University of Carthage, Tunisia. Electronic address: salwamaster@gmail.com.
authors.forename: Salwa
authors.initials: S
authors.lastname: Bouabdallah
authors.affiliation: Laboratory of Transmission, Control and Immunobiology of Infection (11 LR IPT 02), Pasteur Institute, Tunisia.
authors.forename: Rabiaa-M
authors.initials: RM
authors.lastname: Sghaier
authors.affiliation: Laboratory of Bioactive Substances, Biotechnology Centre in Borj-Cedria Technopol, BP.901, Hammam-lif, 2050, Tunisia.
authors.forename: Sawssen
authors.initials: S
authors.lastname: Selmi
authors.affiliation: Laboratory of Molecular Interactions and Chemical Reactivity and Photochemical UMR CNRS 5623, University of Toulouse, University of Paul Sabatier, 118 Route de Narbonne, F-31062 Toulouse, France.
authors.forename: Daycem
authors.initials: D
authors.lastname: Khlifi
authors.affiliation: Laboratory of Transmission, Control and Immunobiology of Infection (11 LR IPT 02), Pasteur Institute, Tunisia.
authors.forename: Dhafer
authors.initials: D
authors.lastname: Laouini
authors.affiliation: Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta, University of Carthage, Tunisia.
authors.forename: Mossadok
authors.initials: M
authors.lastname: Ben-Attia
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts
keywords: ATCM; Anti-cancer; Cyclotrisiloxane; GC–MS; Gokshur; MTTassay; RP-HPLC