title: Distribution of Carcinogenic Human Papillomavirus Genotypes and Association to Cervical Lesions among Women in Fez (Morocco).
abstract: OBJECTIVE
  To determine the distribution of cervical high-risk human papillomavirus genotypes and their association to cellular abnormalities in women from Fez and its neighborhood.
  
  
  METHODS
  Women attending the Hassan II University Hospital for cervical pap smears were recruited after an informed consent. Interviews and two cervical samples were performed for each woman. Cervical samples were used for cytological analysis and HPV DNA detection. HPV was typed using a method based on multiplex PCR with fluorescently labeled specific primers followed by capillary electrophoresis. The study was approved by the ethics committee of the Faculty of Medicine and Pharmacy of Fez.
  
  
  RESULTS
  The HPV prevalence in the studied population was 43.1% and the most prevalent types were HPV 53 (23 cases); HPV 16 (20 cases); HPV 35 (18 cases); HPV 51 (10 cases) and HPV 56 (7 cases). From the 619 confirmed pap smears, 20% were abnormal. The cytological abnormalities were significantly associated to HPV infection, women age, number of pregnancies and parity (p < 0.05).
  
  
  CONCLUSIONS
  More attention should be given to HPV in Morocco because it represents an important public health concern. The distribution of carcinogenic HPV types in the studied population is different from the data in other regions but epidemiological studies in other Moroccan regions are required.
authors.affiliation: Laboratoire de Microbiologie et de Biologie moléculaire, Faculté de Médecine et de Pharmacie de Fès (FMPF), Université Sidi Mohammed Ben Abdellah (USMBA), Fez, Morocco.|Laboratoire de Biotechnologies, Faculté des Sciences Dhar El Mahraz, USMBA, Fez, Morocco.
authors.forename: Tiatou
authors.initials: T
authors.lastname: Souho
authors.affiliation: Laboratoire Central d'Analyse Médicale, CHU Hassan II, Fez, Morocco.
authors.forename: Hinde
authors.initials: H
authors.lastname: El Fatemi
authors.affiliation: Laboratoire de Microbiologie et de Biologie moléculaire, Faculté de Médecine et de Pharmacie de Fès (FMPF), Université Sidi Mohammed Ben Abdellah (USMBA), Fez, Morocco.|Laboratoire de Biotechnologies, Faculté des Sciences Dhar El Mahraz, USMBA, Fez, Morocco.
authors.forename: Safae
authors.initials: S
authors.lastname: Karim
authors.affiliation: Laboratoire d'épidémiologie, Recherche Clinique et Santé Communautaire, FMPF, USMBA, Fez, Morocco.
authors.forename: Karima
authors.initials: K
authors.lastname: El Rhazi
authors.affiliation: Service de Gynecologie-Obstetrique I, CHU Hassan II, Fez, Morocco.
authors.forename: Chahrazed
authors.initials: C
authors.lastname: Bouchikhi
authors.affiliation: Service de Gynecologie-Obstetrique I, CHU Hassan II, Fez, Morocco.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Banani
authors.affiliation: Service de Gynecologie-Obstetrique II, CHU Hassan II, Fez, Morocco.
authors.forename: Moulay Abdelilah
authors.initials: MA
authors.lastname: Melhouf
authors.affiliation: Laboratoire de Biotechnologies, Faculté des Sciences Dhar El Mahraz, USMBA, Fez, Morocco.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Benlemlih
authors.affiliation: Laboratoire de Microbiologie et de Biologie moléculaire, Faculté de Médecine et de Pharmacie de Fès (FMPF), Université Sidi Mohammed Ben Abdellah (USMBA), Fez, Morocco.|Equipe micro-organismes, génomique et Facteurs Oncogènes, Laboratoire de Pathologie Humaine, Biomédecine et Environnement, FMPF, USMBA, Fez, Morocco.
authors.forename: Bahia
authors.initials: B
authors.lastname: Bennani
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009018:Morocco; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral