title: A novel strain of Lactobacillus mucosae isolated from a Gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in D-galactose-induced aging mice.
abstract: Twelve isolates isolated from the gastrointestinal tracts of Gaotian villagers in China, who had a lifespan of 92 yr, were examined for their antioxidants using free radical scavenging activity and 2,2-diphenyl-1-picrylhydrazyl. Three strains (i.e., Lactobacillus mucosae LMU1001, and Lactobacillus plantarum LPL0902 and LPL0302) were selected as candidates to prepare yogurt for testing their antioxidants in a model of d-galactose-induced aging mice, with vitamin C as a positive control. The results showed that L. mucosae LMU1001 was the best strain, which had similar in vivo antioxidant activity as vitamin C. A significant increase was found in the activities of glutathione peroxidase in serum and total superoxide dismutase in the liver, and a decrease in the level of malondialdehyde in serum. Regarding mRNA expression level detected quantitatively by real-time PCR, we observed that L. mucosae LMU1001 significantly upregulated antioxidant genes (i.e., MT1A and MT1M in HT-29 and Caco-2) and those genes (i.e., MT1, MT2, GPx1, and GPx2) in the intestinal tract of the model mice. Hence, this strain could be considered as a potential probiotic lactic acid bacterium for improving antioxidant levels in functional foods.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China; Institute of Applied Agricultural Microbiology, Jiangxi Academy of Agricultural Sciences, 602 Nanlian Road, Nanchang, Jiangxi 330200, P. R. China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China.
authors.forename: Yaoping
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China.
authors.forename: Dengyuan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Food and Nutritional Science, School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Shah
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China. Electronic address: ziwu211@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, P. R. China. Electronic address: weihua114@live.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
mesh_terms: D000369:Aged, 80 and over; D000375:Aging / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D018938:Caco-2 Cells; D002681:China; D055951:Functional Food; D005690:Galactose / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology; D008099:Liver / Q000201:enzymology; D008136:Longevity; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D023421:Models, Animal; D019936:Probiotics; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005690:Galactose
keywords: 2,2-diphenyl-1-picrylhydrazyl; antioxidant activity; d-galactose-induced aging mice; hydroxyl radical; reducing power