title: RepA Protein Encoded by Oat dwarf virus Elicits a Temperature-Sensitive Hypersensitive Response-Type Cell Death That Involves Jasmonic Acid-Dependent Signaling.
abstract: The hypersensitive response (HR) is a component of disease resistance that is often induced by pathogen infection, but essentially no information is available for members of the destructive mastreviruses. We have investigated an HR-type response elicited in Nicotiana species by Oat dwarf virus (ODV) and have found that expression of the ODV RepA protein but not other ODV-encoded proteins elicits the HR-type cell death associated with a burst of H2O2. Deletion mutagenesis indicates that the first nine amino acids (aa) at the N terminus of RepA and the two regions located between aa residues 173 and 195 and between aa residues 241 and 260 near the C terminus are essential for HR-type cell-death elicitation. Confocal and electron microscopy showed that the RepA protein is localized in the nuclei of plant cells and might contain bipartite nuclear localization signals. The HR-like lesions mediated by RepA were inhibited by temperatures above 30°C and involvement of jasmonic acid (JA) in HR was identified by gain- and loss-of-function experiments. To our knowledge, this is the first report of an elicitor of HR-type cell death from mastreviruses.
authors.affiliation: 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China;
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Qian
authors.affiliation: 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China;|2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China;
authors.forename: Huwei
authors.initials: H
authors.lastname: Hou
authors.affiliation: 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China;
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Shen
authors.affiliation: 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China;
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Cai
authors.affiliation: 3 Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, U.S.A.
authors.forename: Garry
authors.initials: G
authors.lastname: Sunter
authors.affiliation: 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China;|2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China;
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
mesh_terms: D016923:Cell Death / Q000187:drug effects*; D003517:Cyclopentanes / Q000378:metabolism*; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D006358:Hot Temperature; D054883:Oxylipins / Q000378:metabolism*; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D015398:Signal Transduction / Q000502:physiology; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D014764:Viral Proteins; C011006:jasmonic acid