title: A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection.
abstract: Based on specific aptamer binding properties, a strategy for adenosine, dopamine and 17β-estradiol detection was realised by employing Ru complex and quantum dots (QDs) as fluorescence probes. Ru complex, which could quench the fluorescence of QDs, preferred to bind with aptamer DNA and resulted in the fluorescence rise of QDs. When the aptamer DNA was incubated with the target first, it could not bind with Ru complex and the fluorescence of QDs was quenched. Under the optimal condition, the fluorescence intensity was linearly proportional to the concentration of adenosine, dopamine and 17β-estradiol with a limit of detection (LOD) of 101 nM, 19 nM and 37 nM, respectively. The experiments in fetal bovine serum were also carried out with good results. This universal method was rapid, label-free, low-cost, easy-operating and highly repeatable for the detection of adenosine, dopamine and 17β-estradiol. Qualitative detection by naked eyes was also available without complex instruments. It could also be extended to detect various analytes, such as metal ions, proteins and small molecules by using appropriate aptamers.
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China. Electronic address: shishuo@tongji.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Ruru
authors.initials: R
authors.lastname: Gao
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Zang
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China. Electronic address: tmyao@tongji.edu.cn.
authors.forename: Tianming
authors.initials: T
authors.lastname: Yao
mesh_terms: D000241:Adenosine / Q000097:blood*; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D002417:Cattle; D004298:Dopamine / Q000097:blood*; D004958:Estradiol / Q000097:blood*; D005453:Fluorescence; D045663:Quantum Dots; D012428:Ruthenium / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D004958:Estradiol; D012428:Ruthenium; D000241:Adenosine; D004298:Dopamine
keywords: 17β-estradiol; Adenosine; Dopamine; Fluorescence; Quantum dots; Ru complex