title: Photodynamic Anticancer Activity of CoFe2O4 Nanoparticles Conjugated with Hematoporphyrin.
abstract: This work reports the synthesis and the characterization of water-soluble and biocompatible photosensitizer (PS)-conjugated magnetic nanoparticles composed of a cobalt ferrite (CoFe2O4) magnetic core coated with a biocompatible hematoporphyrin (HP) shell. The photo-functional cobalt ferrite magnetic nanoparticles (CoFe2O4@HP) were uniform in size, stable against PS leaching, and highly efficient in the photo-generation of cytotoxic singlet oxygen under visible light. With the CoFe2O4@HP, we acquired in vitro MR images of cancer cells (PC-3) and confirmed good biocompatibility of the CoFe2O4@HP in both normal and cancer cells. In addition, we confirmed the potential of the CoFe2O4@HP as an agent for photodynamic therapy (PDT) applications. The photodynamic anticancer activities in 25, 50, and 100 μg/mL of CoFe2O4@HP were measured and found to exceed 99% (99.0, 99.4, and 99.5%) (p < 0.002). The photodynamic anticancer activity was 81.8% (p < 0.003). From these results, we suggest that our CoFe2O4@HP can be used safely as a type of photodynamic cancer therapy with potential as a therapeutic agent having good biocompatibility. Moreover, these photo-functional magnetic nanoparticles are highly promising for applications in versatile imaging diagnosis and as a therapy tool in biomedical engineering.
authors.forename: Bong Joo
authors.initials: BJ
authors.lastname: Park
authors.forename: Kyong-Hoon
authors.initials: KH
authors.lastname: Choi
authors.forename: Ki Chang
authors.initials: KC
authors.lastname: Nam
authors.forename: Jeeeun
authors.initials: J
authors.lastname: Min
authors.forename: Kyu-Dong
authors.initials: KD
authors.lastname: Lee
authors.forename: Han Sup
authors.initials: HS
authors.lastname: Uhm
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Choi
authors.forename: Ho-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jin-Seung
authors.initials: JS
authors.lastname: Jung
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology; D006415:Hematoporphyrins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods*
chemical_list: D005290:Ferric Compounds; D006415:Hematoporphyrins; C001215:ferrite; D003035:Cobalt