title: Evaluation of Caesalpinia bonducella flower extract for anti-inflammatory action in rats and its high performance thin layer chromatography chemical fingerprinting.
abstract: OBJECTIVE
  The study is aimed to evaluate anti-inflammatory activity of Caesalpinia bonducella Fleming (Caesalpiniaceae) flower extract (CBFE) and to study its effect on radiographic outcome in adjuvant induced arthritis and authentication by high performance thin layer chromatography (HPTLC) chemical fingerprinting.
  
  
  METHODS
  CBFE was administered orally (30, 100, and 300 mg/kg b.wt.) and tested for its anti-inflammatory activity in carrageenan-induced inflammation, cotton pellet induced chronic granulomatous inflammation and autacoids-induced inflammation. Effect on radiographic outcome was tested in adjuvant-induced arthritis. CBFE was HPTLC fingerprinted in suitable solvent system.
  
  
  RESULTS
  In carrageenan-induced inflammation, CBFE produced significant inhibition in edema volume at all the doses (30, 100 and 300 mg/kg b.wt.) and percentage of inhibition was 28.68, 31.00, and 22.48, respectively as compared to control at 5 h of its administration. In cotton pellet granuloma assay, CBFE significantly decreased the granuloma weight at 300 mg/kg dose level by 22.53%. CBFE (300 mg/kg) caused significant inhibition by 37.5, 44.44, and 35.29% edema volume, at ½, 1 and 3 h after 5-hydroxytryptamine injection, respectively. Radiographic score of animals treated with 300 mg/kg CBFE was significantly decreased when compared to arthritic control animals.
  
  
  CONCLUSIONS
  The extract was found to possess significant anti-inflammatory activity. CBFE treatment improved the bony architecture in adjuvant-induced arthritis in rats. The developed HPTLC fingerprint would be helpful in the authentication of C. bonducella flower extract.
authors.affiliation: Department of Pharmacology and Chemistry, Captain Srinivasa Murti Research Institute for Ayurveda and Siddha Drug Development, CCRAS, M/o AYUSH, (Govt. of India), Chennai, Tamil Nadu, India.
authors.forename: Rathinam
authors.initials: R
authors.lastname: Arunadevi
authors.affiliation: Department of Pharmacology and Chemistry, Captain Srinivasa Murti Research Institute for Ayurveda and Siddha Drug Development, CCRAS, M/o AYUSH, (Govt. of India), Chennai, Tamil Nadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Murugammal
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Surendra Kumar
authors.initials: SK
authors.lastname: Tandan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D029911:Caesalpinia / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D020834:Ethnopharmacology; D005260:Female; D035264:Flowers / Q000737:chemistry*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D007194:India; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry / Q000627:therapeutic use*; D011859:Radiography; D011897:Random Allocation; D017208:Rats, Wistar; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts
keywords: 5-hydroxy tryptamine; Caesalpinia bonducella; carrageenan-induced inflammation; cotton pellet granuloma; high performance thin layer chromatography; radiography