title: Bone marrow mesenchymal stem cells combined with minocycline improve spinal cord injury in a rat model.
abstract: The aims of this study were to assess that the effects of bone marrow mesenchymal stem cells (BMSCs) combination with minocycline improve spinal cord injury (SCI) in rat model. In the present study, the Wistar rats were randomly divided into five groups: control group, SCI group, BMSCs group, Minocycline group and BMSCs + minocycline group. Basso, Beattie and Bresnahan (BBB) test and MPO activity were used to assess the effect of combination therapy on locomotion and neutrophil infiltration. Inflammation factors, VEGF and BDNF expression, caspase-3 activation, phosphorylation-p38MAPK, proNGF, p75NTR and RhoA expressions were estimated using commercial kits or western blot, respectively. BBB scores were significantly increased and MPO activity was significantly undermined by combination therapy. In addition, combination therapy significantly decreased inflammation factors in SCI rats. Results from western blot showed that combination therapy significantly up-regulated the protein of VEGF and BDNF expression and down-regulated the protein of phosphorylation-p38MAPK, proNGF, p75NTR and RhoA expressions in SCI rats. Combination therapy stimulation also suppressed the caspase-3 activation in SCI rats. These results demonstrated that the effects of bone marrow mesenchymal stem cells combination with minocycline improve SCI in rat model.
authors.affiliation: Department of Spine Surgery, Second Xiangya Hospital of Central South University Changsha 410011, China ; Department of Spine Surgery, The Central Hospital of Zhuzhou City Zhuzhou 412000, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, The Central Hospital of Zhuzhou City Zhuzhou 412000, China ; Second Xiangya Hospital of Central South University Changsha 410011, Hunan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.affiliation: The Third Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Fu
authors.affiliation: The Third Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Spine Surgery, Second Xiangya Hospital of Central South University Changsha 410011, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Lv
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D016026:Bone Marrow Transplantation / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D008911:Minocycline / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000601:surgery*
chemical_list: D000893:Anti-Inflammatory Agents; D008911:Minocycline
keywords: Bone marrow mesenchymal stem cells; VEGF and BDNF; inflammation; minocycline; spinal cord injury