title: Isolation of Staphylococcus microti from milk of dairy cows with mastitis.
abstract: The present paper is a case-report of multiple udder infections in a dairy herd caused by Staphylococcus microti. Over a 22-month period, eleven S. microti isolates from milk samples from 9 cows were collected. The animals experienced subclinical (with one exception) intramammary infections with a high self-cure rate. The identification of the microorganism was carried out by means of two independent approaches: nucleotide sequence analysis of the 16S rRNA gene, as well as some housekeeping genes (sodA, rpoB, dnaJ), and matrix-assisted laser desorption ionization-time of flight mass spectrometry. All S. microti isolates belonged to an apparently single clone (as detected by the RAPD analysis), indicating that the microorganism could adapt, to some degree, to the bovine mammary gland or even spread from cow to cow in a contagious manner. This report is, to our knowledge, the first ever case of bovine mastitis caused by S. microti and the first instance of isolation of this microorganism from domesticated animals.
authors.affiliation: Department of Pathology, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, St. Norwida 31, 50-375 Wrocław, Poland. Electronic address: jaroslaw.krol@up.wroc.pl.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Król
authors.affiliation: Department of Pathology, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, St. Norwida 31, 50-375 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wanecka
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Pl. Grunwaldzki 49, 50-366 Wrocław, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Twardoń
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Pl. Grunwaldzki 49, 50-366 Wrocław, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Mrowiec
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Pl. Grunwaldzki 49, 50-366 Wrocław, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Dropińska
authors.affiliation: Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, St. Norwida 31, 50-375 Wrocław, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bania
authors.affiliation: Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, St. Norwida 31, 50-375 Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Podkowik
authors.affiliation: Department of Immunology of Infectious Diseases, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, St. Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Korzeniowska-Kowal
authors.affiliation: Department of Immunology of Infectious Diseases, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, St. Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Paściak
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000235:genetics / Q000302:isolation & purification*
keywords: Case-report; Dairy cattle; Mastitis; Molecular diagnostics; Staphylococcus microti