title: Pulmonary responses to pathogen-specific antigens in latent Mycobacterium tuberculosis infection.
abstract: In this study, we used ELISPOT to quantify frequencies of bronchoalveolar lavage (BAL) and peripheral blood T cells capable of producing IFNγ in response to PPD, antigen 85B, and Mtb-specific antigens CFP-10 and ESAT-6 in individuals with latent tuberculosis infection (LTBI) and Mtb-naïve controls. Compared to peripheral blood, BAL cells of LTBI subjects displayed significant enrichment for T cells responding to PPD, antigen 85B, and CFP-10, but not to ESAT-6. Baseline BAL cells of LTBI subjects displayed significant production of Mig (CXCL9) in response to PPD, antigen 85B, and CFP-10 as well. These findings suggest that enrichment for Mtb-specific T cells within BAL is not unique to active pulmonary tuberculosis and may, to the contrary, contribute to protection from re-infection in Mtb immune individuals.
authors.affiliation: Division of Pulmonary, Critical Care and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA; Division of Pulmonary, Critical Care and Sleep Medicine, Louis Stokes Cleveland Department of Veterans' Affairs Medical Center, Cleveland, OH, USA.
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Jarvela
authors.affiliation: Division of Pulmonary, Critical Care and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA; Division of Pulmonary, Critical Care and Sleep Medicine, Louis Stokes Cleveland Department of Veterans' Affairs Medical Center, Cleveland, OH, USA.
authors.forename: Lori
authors.initials: L
authors.lastname: Tuscano
authors.affiliation: Division of Pulmonary, Critical Care and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA; Division of Pulmonary, Critical Care and Sleep Medicine, Louis Stokes Cleveland Department of Veterans' Affairs Medical Center, Cleveland, OH, USA.
authors.forename: Hung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Pulmonary, Critical Care and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA; Division of Pulmonary, Critical Care and Sleep Medicine, University Hospitals Case Medical Center, Cleveland, OH, USA; Division of Pulmonary, Critical Care and Sleep Medicine, Louis Stokes Cleveland Department of Veterans' Affairs Medical Center, Cleveland, OH, USA. Electronic address: rfs4@case.edu.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Silver
mesh_terms: D000217:Acyltransferases / Q000276:immunology; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000382:microbiology; D016022:Case-Control Studies; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000276:immunology / Q000378:metabolism; D054370:Chemokine CXCL9 / Q000276:immunology / Q000378:metabolism; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D059425:Interferon-gamma Release Tests; D055985:Latent Tuberculosis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D008168:Lung / Q000276:immunology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D014373:Tuberculin / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C114753:CXCL10 protein, human; C080810:CXCL9 protein, human; D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; C093276:ESAT-6 protein, Mycobacterium tuberculosis; C573415:IFNG protein, human; D014373:Tuberculin; D007371:Interferon-gamma; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: Bronchoalveolar lavage; ELISPOT; Immunodiagnosis; Latent tuberculosis infection; Tuberculosis