title: Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.
abstract: We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.
authors.affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, 92103, United States of America.|Department of Anatomy, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Wenluo
authors.initials: W
authors.lastname: Cao
authors.affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.
authors.forename: Lingna
authors.initials: L
authors.lastname: Li
authors.affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Tran
authors.affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, 92103, United States of America.|Department of Dermatology, Kitasato University, Sagimahara, 228-8555, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kajiura
authors.affiliation: Department of Dermatology, Kitasato University, Sagimahara, 228-8555, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.affiliation: Department of Anatomy, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, 92103, United States of America.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D015925:Cryopreservation; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000637:transplantation*; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D039904:Pluripotent Stem Cells / Q000378:metabolism; D014738:Vibrissae / Q000637:transplantation*