title: [Effects of JAZF1 overexpression on proinflammatory cytokines in hepatocytes induced by palmitic acid].
abstract: OBJECTIVE
  To investigate the effects of JAZF1 overexpression on the pro-inflammatory cytokines in hepatic steatosis.
  
  
  METHODS
  The model of hepatic steatosis was established by incubating hepatocytes with palmitic acid (PA) at 0, 0.125, 0.25, 0.5 and 1 mM dose and for 0, 6, 12, 24 and 48 hours, after which recombinant adenovirus expressing JAZF1 (Ad-JAZF1) was introduced to up-regulate expression. Triglyceride level was measured by GOD. Cell viability was detected by CCK-8. The mRNA and protein expression of TNF-alpha, MCP-1, IL-8 and JAZF1 was examined by RT-PCR, ELISA, and western blotting.
  
  
  RESULTS
  The PA-treated hepatocytes showed dose-dependent significant increases in TNF-alpha, MCP-1 and IL-8 mRNA expression for doses up to 0.25 mM; there were no significant increases for the highest doses of 0.5 and 1 mM. The 0.25 mM PA-treated hepatocytes showed time-dependent significant increases in TNF-alpha, MCP-1 and IL-8 mRNA expressions (FTNF-alpha = 26.51, FMCP-1 = 57.20, FIL-8 = 353.85, P less than 0.01), with the maximum level reached at 12 h and followed by a gradual decrease with longer treatment times. JAZF1 mRNA and protein expression was markedly increased in hepatocytes infected with Ad-JAZF1 (P less than 0.01). However, the AP-treated hepatocytes with JAZF1 overexpression showed down-regulation of TNF-alpha, MCP-1 and IL-8 mRNA expression (decreased by 89.69%, 77.68%, and 83.21%, respectively) and secretion (37%, 37% and 41%, respectively, P less than 0.01).
  
  
  CONCLUSIONS
  Stimulation of hepatocytes by the PA fatty acid in vitro promotes mRNA expression of TNF-alpha, MCP-1 and IL-8, but overexpression of JAZF1 inhibits the PA-induced expression and secretion of these factors.
authors.affiliation: Department of Oncology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Lin
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jia
authors.forename: Gangyi
authors.initials: G
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
mesh_terms: D002470:Cell Survival; D018932:Chemokine CCL2 / Q000378:metabolism; D056970:Co-Repressor Proteins; D016207:Cytokines / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005234:Fatty Liver; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D019308:Palmitic Acid / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D056970:Co-Repressor Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; D016209:Interleukin-8; C430460:JAZF1 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019308:Palmitic Acid