title: Structures and Activity of New Anabaenopeptins Produced by Baltic Sea Cyanobacteria.
abstract: Anabaenopeptins, bioactive cyclic hexapeptides, were isolated by preparative reversed-phase high performance liquid chromatography from an extract of Baltic Sea cyanobacterial bloom material composed of Nodularia spumigena (50%), Aphanizomenon flos-aquae (40%) and Dolichospermum spp. (10%). Five new anabaenopeptins and nine previously known anabaenopeptins were isolated, and their putative structures were determined by tandem mass spectrometry. The activity of the peptides against carboxypeptidase A and protein phosphatase 1 as well as chymotrypsin, trypsin and thrombin was tested. All anabaenopeptins inhibited carboxypeptidase A (apart from one anabaenopeptin variant) and protein phosphatase 1 with varying potency, but no inhibition against chymotrypsin, trypsin and thrombin was observed.
authors.affiliation: Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6 A, 20520 Turku, Finland. lspoof@abo.fi.
authors.forename: Lisa
authors.initials: L
authors.lastname: Spoof
authors.affiliation: Department of Marine Biotechnology, University of Gdańsk, Al. Marszałka Piłsudskiego 46, 81-378 Gdynia, Poland. agata.blaszczyk@ug.edu.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Błaszczyk
authors.affiliation: Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6 A, 20520 Turku, Finland. jussi.meriluoto@abo.fi.
authors.forename: Jussi
authors.initials: J
authors.lastname: Meriluoto
authors.affiliation: Department of Marine Biotechnology, University of Gdańsk, Al. Marszałka Piłsudskiego 46, 81-378 Gdynia, Poland. ma.ceglowska@gmail.com.
authors.forename: Marta
authors.initials: M
authors.lastname: Cegłowska
authors.affiliation: Department of Marine Biotechnology, University of Gdańsk, Al. Marszałka Piłsudskiego 46, 81-378 Gdynia, Poland. biohm@ug.edu.pl.
authors.forename: Hanna
authors.initials: H
authors.lastname: Mazur-Marzec
mesh_terms: D001454:Baltic States; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006801:Humans; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010456:Peptides, Cyclic / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D012623:Seawater; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry; D013917:Thrombin / Q000037:antagonists & inhibitors
chemical_list: D004791:Enzyme Inhibitors; D010456:Peptides, Cyclic; D010744:Phosphoric Monoester Hydrolases; D010196:Pancreatic Elastase; D013917:Thrombin
keywords: Anabaenopeptins; Aphanizomenon flos-aquae; Baltic Sea; Dolichospermum spp.; Nodularia spumigena; carboxypeptidase A; cyanobacteria; nodulapeptins; protein phosphatase 1