title: Investigation of some DNA repair genes association in non small cell lung cancer.
abstract: Ribonucleoside-diphosphate reductase subunit M2, also known as ribonucleotide reductase small subunit, is an enzyme that in humans is encoded by the RRM2 gene and also Ribonucleoside-diphosphate reductase large subunit is an enzyme that in humans is encoded by the RRM1 gene. RRM1 is a gene important in determining tumor phenotype, but also induced the expression of PTEN tumor suppressor gene, cell migration, invasion and metastasis formation, and play a preventive role. ERCC2 DNA repair mechanism is associated in more than 20 genes involved in the NER pathway. The aim of this study is to investigate rs13181 ERCC2 (T>G) (Lys751Gln), rs12806698 RRM1 (-269C>A) and rs6759180 (located in the 5'UTR) RRM2 (10126436G>A) gene polymorphisms by using real time PCR technique in patients with NSCLC. 193 NSCLC cases and 141 healthy control cases were included in this study. A significant difference was found between rs12806698 RRM1 genotype distributions (*p: 0.034) and were determined increases the risk of disease approximately 3.044 times AA genotype having (*p: 0.014 OR: 3.044, 95%CI: 1.205-7,688). A significant difference was found between rs6759180 RRM2 genotype distributions (*p: 0.033) and were determined increases the risk of disease approximately 3.49 times GG genotype having (p: 0,009 OR: 3, 49, %95CI:1.291-9,482). It was found significant difference in serum 8-OHdG levels between patients and controls (*p: 0001).
authors.affiliation: Istanbul University Department of Molecular Medicine, Institute of Experimental Medicine Istanbul Turkey ecoskunpinar@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Coskunpinar
authors.affiliation: Yedikule Chest Disease and Thoracic Surgery Training and Research Hospital, Third clinic Department of Pulmonology Istanbul Turkey.
authors.forename: P
authors.initials: P
authors.lastname: Yildiz
authors.affiliation: Yedikule Chest Disease and Thoracic Surgery Training and Research Hospital, Third clinic Department of Pulmonology Istanbul Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Aynaci
authors.affiliation: Istanbul University Department of Thoracic Surgery, Cerrahpasa Medical School, Istanbul Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Turna
authors.affiliation: Istanbul University Department of Molecular Medicine, Institute of Experimental Medicine Istanbul Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Musteri Oltulu
authors.affiliation: Istanbul University Department of Thoracic Surgery, Cerrahpasa Medical School, Istanbul Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Hekimoglu
authors.affiliation: Yeditepe University Department of Medical Biology, Faculty of Medicine Istanbul Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Isbir
authors.affiliation: Istanbul University Department of Molecular Medicine, Institute of Experimental Medicine Istanbul Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Yaylim
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012262:Ribonucleoside Diphosphate Reductase / Q000235:genetics*; D012306:Risk; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D025521:Tumor Suppressor Proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; C093713:ribonucleotide reductase M2; C475286:RRM1 protein, human; D012262:Ribonucleoside Diphosphate Reductase; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D003849:Deoxyguanosine