title: Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking, and targeted delivery of adipose tissue-derived stem cells.
abstract: Tracking and monitoring of cells in vivo after transplantation can provide crucial information for stem cell therapy. Magnetic resonance imaging (MRI) combined with contrast agents is believed to be an effective and non-invasive technique for cell tracking in living bodies. However, commercial superparamagnetic iron oxide nanoparticles (SPIONs) applied to label cells suffer from shortages such as potential toxicity, low labeling efficiency, and low contrast enhancing. Herein, the adipose tissue-derived stem cells (ADSCs) were efficiently labeled with SPIONs coated with poly (dopamine) (SPIONs cluster@PDA), without affecting their viability, proliferation, apoptosis, surface marker expression, as well as their self-renew ability and multi-differentiation potential. The labeled cells transplanted into the mice through tail intravenous injection exhibited a negative enhancement of the MRI signal in the damaged liver-induced by carbon tetrachloride, and subsequently these homed ADSCs with SPIONs cluster@PDA labeling exhibited excellent repair effects to the damaged liver. Moreover, the enhanced target-homing to tissue of interest and repair effects of SPIONs cluster@PDA-labeled ADSCs could be achieved by use of external magnetic field in the excisional skin wound mice model. Therefore, we provide a facile, safe, noninvasive and sensitive method for external magnetic field targeted delivery and MRI based tracking of transplanted cells in vivo.
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.
authors.forename: Naishun
authors.initials: N
authors.lastname: Liao
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, Fuzong Clinical College, Fujian Medical University, Fuzhou 350001, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Pan
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China.
authors.forename: Jiumao
authors.initials: J
authors.lastname: Lin
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China.
authors.forename: Zuanfang
authors.initials: Z
authors.lastname: Li
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.
authors.forename: Da
authors.initials: D
authors.lastname: Zhang
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.
authors.forename: Youshi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.|The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, P.R. China.|Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350007, P.R. China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D017209:Apoptosis; D015415:Biomarkers; D002465:Cell Movement; D002470:Cell Survival; D058948:Cell Tracking / Q000379:methods; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D004195:Disease Models, Animal; D015870:Gene Expression; D016130:Immunophenotyping; D007211:Indoles / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D060526:Magnetic Fields; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D010087:Oxides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D033581:Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D007211:Indoles; D058185:Magnetite Nanoparticles; D010087:Oxides; D011108:Polymers; C568283:polydopamine; D007501:Iron