title: [Lymphocyte subsets, dendritic cells and cytokine profiles in mice with melanoma treated with Uncaria tomentosa].
abstract: OBJECTIVE
  To evaluate the immunomodulatory effect on lymphocyte subsets, dendritic cells (DC), Th1 / Th2 / Th17 and inflammatory cytokines on systemic level and/or in the tumor microenvironment of mice with or without melanoma.
  
  
  METHODS
  Peripheral blood and/or primary tumors samples were obtained of mice with B16 melanoma treated or not with a hydroalcoholic extract of Uncaria tomentosa (UT) with 5.03% of pentacyclic oxindole alkaloids (UT-POA) obtained from the bark of the plant. All cell assays and cytokine measurements were performed by flow cytometry.
  
  
  RESULTS
  UT-POA systemically increased CD4/CD8a relation while cell activation was inversely proportional; increased the proportion of DCm; induced a pro-inflammatory Th1 profile and reduced Th17 response. TNF-α and IL-17A positively and negatively correlated with CD4/CD8a relation.
  
  
  CONCLUSIONS
  The increase of Th1 (TNF-α) may result in the increase of CD4 or M1 macrophage activation. Although UT-POA shows increased DCm, is not dose-dependent. Th17(IL-17A) decreased can support the function of CD8a lymphocytes. UT-POA shows better systemic immunomodulatory effects than intratumoral.
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Iván
authors.initials: I
authors.lastname: Lozada-Requena
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: César
authors.initials: C
authors.lastname: Núñez
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Yubell
authors.initials: Y
authors.lastname: Alvárez
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Laura
authors.initials: L
authors.lastname: Kahn
authors.affiliation: Laboratorio de Inmunología, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: José
authors.initials: J
authors.lastname: Aguilar
mesh_terms: D000818:Animals; D020948:Cat's Claw; D016207:Cytokines; D003713:Dendritic Cells; D016131:Lymphocyte Subsets; D008545:Melanoma / Q000188:drug therapy*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000188:drug therapy*
chemical_list: D016207:Cytokines; D010936:Plant Extracts