title: Sangxingtang inhibits the inflammation of LPS-induced acute lung injury in mice by down-regulating the MAPK/NF-κB pathway.
abstract: In the present study, we investigated anti-inflammatory effects of Sangxingtang (SXT) on acute lung injury using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was performed. The degree of lung edema was evaluated by measuring the wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) and myeloperoxidase (MPO) activities were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were assayed by the enzyme-linked immunosorbent assay methods. Pathological changes of lung tissues were observed by Hematoxylin and eosin (HE) staining. The inflammatory signaling pathway-related proteins nuclear factor mitogen activated protein kinases (P38MAPK), extracellular regulated protein kinases (Erk), c-Jun N-terminal kinase (Jnk) and nuclear transcription factor (NF-κB) p65 expressions were measured by Western blotting. Our results showed that the treatment with the SXT markedly attenuated the inflammatory cell numbers in the BALF, decreased the levels of P-P38MAPK, P-Erk, P-Jnk and P-NF-κB p65 and the total protein levels in lungs, improved the SOD activity and inhibited the MPO activity. Histological studies demonstrated that SXT substantially reduced the LPS-induced neutrophils in lung tissues, compared with the untreated LPS group. In conclusion, our results indicated that SXT had protective effects on LPS-induced ALI in mice.
authors.affiliation: School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China; College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Tian-Zhu
authors.initials: TZ
authors.lastname: Zhang
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China. Electronic address: zhangtz452@126.com.
authors.forename: Shi-Hai
authors.initials: SH
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China.
authors.forename: Jin-Fu
authors.initials: JF
authors.lastname: Yao
authors.affiliation: School of Life Science, Peking University, Beijing 100871, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, 210009, China.
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: Yan
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000009:adverse effects; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D020928:Mitogen-Activated Protein Kinases
keywords: Inflammation; LPS-induced acute lung injury; MAPK/NF-κB pathway; Sangxingtang