title: Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood.
abstract: The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.|Human Biotech Co. Ltd., Jinju 52839, Korea.
authors.forename: Hyo-Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.affiliation: Gyeongnam Wildlife Center, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kang
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Se-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Kwang-Dong
authors.initials: KD
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Hee-Chun
authors.initials: HC
authors.lastname: Lee
authors.affiliation: School of Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Ji-Kwon
authors.initials: JK
authors.lastname: Park
authors.affiliation: School of Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Won-Young
authors.initials: WY
authors.lastname: Paik
authors.affiliation: College of Veterinary Medicine, Western University, Pomona, CA 91766-1854, USA.
authors.forename: Lyon
authors.initials: L
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.|Gyeongnam Wildlife Center, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Seong-Chan
authors.initials: SC
authors.lastname: Yeon
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006680:HLA Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice
chemical_list: D006680:HLA Antigens
keywords: apoptosis; human leukocyte antigen; human umbilical cord blood; immunophenotypes; mesenchymal stem cells