title: Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging.
abstract: We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.
authors.affiliation: Department of Mechanical Engineering, Yonsei University., 50 Yonsei-ro, Seodaemoon-gu, Seoul, 120-749, South Korea.
authors.forename: Donghak
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Yonsei University., 50 Yonsei-ro, Seodaemoon-gu, Seoul, 120-749, South Korea.
authors.forename: Suho
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Department of Mechanical Engineering, Yonsei University., 50 Yonsei-ro, Seodaemoon-gu, Seoul, 120-749, South Korea.
authors.forename: Uihan
authors.initials: U
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Yonsei University., 50 Yonsei-ro, Seodaemoon-gu, Seoul, 120-749, South Korea.
authors.forename: Daeseong
authors.initials: D
authors.lastname: Jung
authors.affiliation: Department of Mechanical Engineering, Yonsei University., 50 Yonsei-ro, Seodaemoon-gu, Seoul, 120-749, South Korea.
authors.forename: Chulmin
authors.initials: C
authors.lastname: Joo
keywords: (100.5070) Phase retrieval; (110.0180) Microscopy; (110.1758) Computational imaging