title: Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China.
abstract: Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76%, 76%, and 78% to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China.
authors.forename: Dongzi
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zong
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Wei
authors.affiliation: US Department of Agriculture, ARS, Molecular Plant Pathology Laboratory, Room 214, Building 004 BARC West, Beltsville, MD, 20705, USA. rose.hammond@ars.usda.gov.
authors.forename: Rosemarie W
authors.initials: RW
authors.lastname: Hammond
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China. qzliu001@126.com.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Liu
mesh_terms: D002681:China; D029381:Closteroviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016000:Cluster Analysis; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D000068220:Prunus avium / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral