title: Discrepancy in compliance between the clinical and genetic diagnosis of choroidal hypoplasia in Danish Rough Collies and Shetland Sheepdogs.
abstract: Collie eye anomaly (CEA) is a congenital, inherited ocular disorder which is widespread in herding breeds. Clinically, the two major lesions associated with CEA are choroidal hypoplasia (CH) and coloboma, and both lesions are diagnosed based on ophthalmological examination. A 7.8-kb intronic deletion in the gene encoding non-homologous end-joining factor 1 (NHEJ1) has been reported to be the causative mutation underlying CH when present in the homozygous state. In this study, we have investigated the compliance between the clinical and genetic diagnosis of CH in the Danish Rough Collie and Shetland Sheepdog populations. Our results show that the deletion in NHEJ1 is not predictive for CH in the Danish Rough Collie population, whereas the clinical and genetic diagnosis is in accordance with each other in the Shetland Sheepdog population. Based on these results, it can be concluded that the intronic deletion in NHEJ1 is not the causative mutation but, rather, a marker linked to the locus underlying the trait in some populations but linked to both the wild-type and CH-causing locus in most dogs in the Danish Rough Collie population.
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Fredholm
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
authors.forename: R C
authors.initials: RC
authors.lastname: Larsen
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Jönsson
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
authors.forename: M A
authors.initials: MA
authors.lastname: Söderlund
authors.affiliation: Haslev Dyreklinik, Baekvej 7, 4690, Haslev, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Hardon
authors.affiliation: The Danish Kennel Club, Parkvej 1, 2680, Solrød Strand, Denmark.
authors.forename: H F
authors.initials: HF
authors.lastname: Proschowsky
mesh_terms: D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D045643:DNA Repair Enzymes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004283:Dog Diseases / Q000175:diagnosis / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D015785:Eye Diseases, Hereditary / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D008040:Genetic Linkage; D007438:Introns; D010641:Phenotype; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
chemical_list: D004268:DNA-Binding Proteins; D045643:DNA Repair Enzymes
keywords: Rough Collies; Shetland Sheepdogs; choroidal hypoplasia