title: Therapeutic role of quercetin on oxidative damage induced by acrylamide in rat brain.
abstract: Context Quercetin (QE), a bioflavonoid present abundantly in fruits and vegetables, has been reported to possess antioxidant properties. Acrylamide (ACR) is formed in foods during cooking and is known to be neurotoxic. Objective The present study was designed to evaluate the protective effect of QE against neurotoxicity induced by ACR. Materials and methods Four groups of Wistar rats consisting of six rats each: (i) control group; (ii) acrylamide treated group (50 mg/kg body weight as single dose); (iii) quercetin group: rats were treated intraperitoneally (i.p.) with QE (10 mg/kg body weight alone every day for 5 d); (iv) quercetin + acrylamide group: quercetin (10 mg/kg bw) was given i.p. every day for 5 d followed by acrylamide i.p. injection (50 mg/kg bw) on fifth day (single dose). Rats were killed after 48 h. Results Administration of ACR (50 mg/kg bw) in Wistar rats resulted in significant increase of dopamine, interferon-γ and 8-hydroxyguanosine with concomitant decrease of serotonin (p < 0.001) in the rat brain. Treatment of rats with QE intraperitonealy (10 mg/kg body weight) before ACR assault resulted in the diminution of ACR-mediated neurotoxicity as evident from decreased levels of dopamine, interferon-γ (p < 0.001) and 8-hydroxyguanosine with concomitant restoration of serotonin levels (p < 0.001). Discussion and conclusion On the basis of the above results, the present study suggests that quercetin may be a potential therapeutic agent for restoration of oxidative damage to neurons.
authors.affiliation: a Department of Biochemistry , College of Science, King Saud University , Riyadh , Kingdom of Saudi Arabia ;
authors.forename: Seema
authors.initials: S
authors.lastname: Zargar
authors.affiliation: a Department of Biochemistry , College of Science, King Saud University , Riyadh , Kingdom of Saudi Arabia ;
authors.forename: Nikhat Jamal
authors.initials: NJ
authors.lastname: Siddiqi
authors.affiliation: b Department of Clinical Sciences , College of Applied Medical Science, King Saud University , Riyadh , Saudi Arabia.
authors.forename: Sabah
authors.initials: S
authors.lastname: Ansar
authors.affiliation: a Department of Biochemistry , College of Science, King Saud University , Riyadh , Kingdom of Saudi Arabia ;
authors.forename: Maha Saleh
authors.initials: MS
authors.lastname: Alsulaimani
authors.affiliation: a Department of Biochemistry , College of Science, King Saud University , Riyadh , Kingdom of Saudi Arabia ;
authors.forename: Afaf K
authors.initials: AK
authors.lastname: El Ansary
mesh_terms: D020106:Acrylamide; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019610:Cytoprotection; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D006151:Guanosine; D020106:Acrylamide; D012701:Serotonin; C046215:8-hydroxyguanosine; D007371:Interferon-gamma; D011794:Quercetin; D004298:Dopamine
keywords: Dopamine; interferon-γ; neurotoxicity; neurotransmitters; oxidative stress