title: [Protective effect of propofol against cerebral ischemic/reperfusion injury may involve inhibition of gap junction].
abstract: OBJECTIVE
  To investigate the protective effect of propofol against focal cerebral ischemia/reperfusion (I/R) injury in rats and its relation with gap junction.
  
  
  METHODS
  Seventy adult male SD rats were randomly divided into sham-operated group, I/R group, low-, moderate-, and high-dose propofol groups (25, 50, 100 mg/kg; P25, P50, P100 groups, respectively), I/R+CBX group and P100+CBX group. Thread occlusion was used to induce middle cerebral artery occlusion (MCAO) in the mice for 2 h followed by reperfusion for 24 h. Longa's scores were used to evaluate the neurological behavior of the rats. TTC staining was used to measure the cerebral infarction volume and Western blotting was performed to detect the expressions of Cx43, PKC, Bax, and Bcl-2 in the brain of the rats.
  
  
  RESULTS
  Compared with the I/R group, the rats pretreated with moderate and high doses of propofol showed significantly reduced neurological behavior scores and cerebral infarction volume percentage, and the effect was more obvious in high-dose propofol pretreatment group. CBX obviously enhanced the protective effect of propofol against I/R injury. Compared with those in the sham-operated group, the protein expression of Cx43 and the Bax/Bcl-2 ratio were increased and the protein expression of PKC was reduced in I/R group, and these changes were significantly reversed by high-dose propofol pretreatment; the effects of propofol were further enhanced by CBX.
  
  
  CONCLUSIONS
  The protective effect of propofol against cerebral I/R injury may involve the inhibition of the gap junction via PKC signaling and by reducing the Bax/Bcl-2 ratio.
authors.affiliation: Department of Pathology, Bengbu Medical College, Bengbu 233030, China. E-mail: 15215520282@126.com.
authors.forename: Zongbing
authors.initials: Z
authors.lastname: Fan
authors.forename: Xuhui
authors.initials: X
authors.lastname: Tong
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Yinling
authors.initials: Y
authors.lastname: Chen
authors.forename: Haoang
authors.initials: H
authors.lastname: Liu
authors.forename: Shuying
authors.initials: S
authors.lastname: Dong
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000517:prevention & control*; D018031:Connexin 43 / Q000378:metabolism; D017629:Gap Junctions; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D015742:Propofol / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D018031:Connexin 43; C580592:Gja1 protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D011493:Protein Kinase C; D015742:Propofol