title: [Effect of ADAM10 Inhibitor GI254023X on Proliferation and Apoptosis of Multiple Myeloma H929 Cells and Its Possible Mechanisms].
abstract: OBJECTIVE
  To investigate the effect of ADAM10 inhibitor GI254023X on the proliferation and apoptosis of multiple myeloma H929 cell line and its mechanisms.
  
  
  METHODS
  H929 cells were treated with different concentrations of GI254023X, the proliferation-inhibitive curve was assayed and plotted by CCK-8 method, the cell viability and apoptosis were detected by flow cytometry with Annexin V/7-AAD double staining. The cleavage of Notch1 protein (cleaved notch1) was determined by Western blot. The transcripts of Notch1 target gene Hes-1 were detected by real-time PCR.
  
  
  RESULTS
  The GI254023X inhibited the proliferation of H929 cells in the time- and dose- dependent manners. As compared with the control group, the apoptosis of cells increased along with enhancement of GI254023X concentration; The expression of cleaved Notch1 was down-regulated after the treatment with GI254023X. The levels of Hes-1 mRNA transcripts in H929 cells was reduced in GI254023X treated group.
  
  
  CONCLUSIONS
  GI254023X can remarkably inhibit the proliferation and induce the apoptosis of H929 cells. Its mechanism may be associated with inbihition of Notch1 activation.
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Chen
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Guo-Qin
authors.initials: GQ
authors.lastname: Fan
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Zhi-Yao
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Bing-Yun
authors.initials: BY
authors.lastname: Zhang
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Yan
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Hu-Jun
authors.initials: HJ
authors.lastname: Li
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
authors.affiliation: Xuzhou Institute of Hematology, Xuzhou Medical College; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail: lizhenyumd@163.com.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
mesh_terms: D051722:ADAM Proteins; D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004151:Dipeptides; D015536:Down-Regulation; D006801:Humans; D006877:Hydroxamic Acids; D008565:Membrane Proteins; D009101:Multiple Myeloma; D051881:Receptor, Notch1
chemical_list: C555398:3-(formylhydroxyamino)-2-(3-phenyl-1-propyl)butanoic acid (2,2-dimethyl-1-methylcarbamoyl-1-propyl)amide; D004151:Dipeptides; D006877:Hydroxamic Acids; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human