title: [Adipose-derived stem cell transplantation inhibits the expression of Nogo-A in the perilesional cortex of rats with focal cerebral ischemia].
abstract: OBJECTIVE
  To explore the effect of adipose-derived stem cells (ADSCs) transplantation on the expression of neurite outgrowth inhibitor-A (Nogo-A) after cerebral ischemia in middle cerebral artery occlusion (MCAO) rats.
  
  
  METHODS
  ADSCs were harvested and purified, and then MCAO models were established in rats by the suture method. Rats were divided into sham operation group, MCAO model group and ADSCs-injected group. One day after MCAO, ADSCs were injected into the lateral ventricle of the ADSC group rats and the same does of PBS was given to model group rats. At day 4, 7 and 14 after MCAO, the rats were decapitated to detect the Nogo-A expression in the perilesional cortex by reverse transcription PCR, immunohistochemistry and Western blotting.
  
  
  RESULTS
  Compared with sham group, the expression of Nogo-A was significantly up-regulated at each time point in both model group and ADSC group. Compared with model group, the expression of Nogo-A was significantly down-regulated at each time point in ADSC group.
  
  
  CONCLUSIONS
  ADSC transplantation could promote the recovery of neurological functions, partly by inhibiting the expression of Nogo-A in MCAO rat's brain.
authors.affiliation: Department of Neurology, First Affiliated Hospital of Quanzhou, Fujian Medical University, Quanzhou 362000, China.
authors.forename: Jiehua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, First Affiliated Hospital of Quanzhou, Fujian Medical University, Quanzhou 362000, China.
authors.forename: Guoqian
authors.initials: G
authors.lastname: Li
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D015153:Blotting, Western; D002545:Brain Ischemia / Q000150:complications; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D005434:Flow Cytometry; D015870:Gene Expression; D018960:Hyaluronan Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000209:etiology / Q000628:therapy*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D000070798:Nogo Proteins; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D018960:Hyaluronan Receptors; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens