title: Concentration of Sonication Fluid through Centrifugation Is Superior to Membrane Filtration for Microbial Diagnosis of Orthopedic Implant-Associated Infection.
abstract: Microbial identification of orthopedic implant-associated infections using sonication fluid (SF) submitted to a concentration step by membrane filtration (SMF) was compared with the standard centrifugation (SC) method. Among 33 retrieved infected implants, sonication identified microorganisms in 26 (78.8%). The sensitivity of SC was higher than that of SMF (78.8% versus 30.3%; P < 0.001).
authors.affiliation: Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil Department of Internal Medicine, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Zitron
authors.affiliation: Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil Department of Internal Medicine, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Tali
authors.initials: T
authors.lastname: Wajsfeld
authors.affiliation: Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil Department of Internal Medicine, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Giselle Burlamaqui
authors.initials: GB
authors.lastname: Klautau
authors.affiliation: Department of Laboratory Medicine and Pathology, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Cely Barreto
authors.initials: CB
authors.lastname: da Silva
authors.affiliation: Department of Laboratory Medicine and Pathology, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Stanley
authors.initials: S
authors.lastname: Nigro
authors.affiliation: Department of Orthopedic Surgery, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Marcelo Tomanik
authors.initials: MT
authors.lastname: Mercadante
authors.affiliation: Department of Orthopedic Surgery, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Giancarlo Cavalli
authors.initials: GC
authors.lastname: Polesello
authors.affiliation: Department of Orthopedic Surgery, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.
authors.forename: Ricardo de Paula Leite
authors.initials: Rde P
authors.lastname: Cury
authors.affiliation: Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil Department of Internal Medicine, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil mcsalles@osite.com.br.
authors.forename: Mauro Jose Costa
authors.initials: MJ
authors.lastname: Salles
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002498:Centrifugation / Q000379:methods*; D002648:Child; D005260:Female; D005374:Filtration / Q000379:methods*; D006801:Humans; D008297:Male; D008828:Microbiological Techniques; D008875:Middle Aged; D019637:Orthopedic Procedures / Q000009:adverse effects*; D019736:Prostheses and Implants / Q000009:adverse effects / Q000382:microbiology; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology*; D055815:Young Adult