title: Improved cellular response of ion modified poly(lactic acid-co-glycolic acid) substrates for mouse fibroblast cells.
abstract: In this report, the effects of argon (Ar) ion irradiation on poly(lactic acid-co-glycolic acid) (PLGA) substrates on biocompatibility were studied. PLGA scaffold substrates were prepared by spin coating glass surfaces with PLGA dissolved in anhydrous chloroform. Previously, we showed that surface modifications of PLGA films using ion irradiation modulate the inherent hydrophobicity of PLGA surface. Here we show that with increasing ion dose (1×10(12) to 1×10(14) ions/cm(2)), hydrophobicity and surface roughness decreased. Biocompatibility for NIH3T3 mouse fibroblast cells was increased by argon irradiation of PLGA substrates. On unirradiated PLGA films, fibroblasts had a longer doubling time and cell densities were 52% lower than controls after 48 h in vitro. Argon irradiated PLGA substrates supported growth rates similar to control. Despite differences in cell cycle kinetics, there was no detectible cytotoxicity observed on any substrate. This demonstrates that argon ion irradiation can be used to tune the surface microstructure and generate substrates that are more compatible for the cell growth and proliferation.
authors.affiliation: Department of Sciences, Wentworth Institute of Technology, Boston MA 02115, United States. Electronic address: aa8381@gmail.com.
authors.forename: Ananta Raj
authors.initials: AR
authors.lastname: Adhikari
authors.affiliation: Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, United States.
authors.forename: Tanya
authors.initials: T
authors.lastname: Geranpayeh
authors.affiliation: Texas Center for Superconductivity, University of Houston, Houston, TX 77204, United States; Department of Physics, University of Houston, Houston, TX 77204, United States.
authors.forename: Wei Kan
authors.initials: WK
authors.lastname: Chu
authors.affiliation: Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, United States; Department of Basic and Vision Sciences, College of Optometry, University of Houston, Houston, TX 77204, United States.
authors.forename: Deborah C
authors.initials: DC
authors.lastname: Otteson
mesh_terms: D000818:Animals; D001128:Argon / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry*; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D001128:Argon
keywords: Cell growth; Polymer nanocomposites; Surface property