title: Nanoencapsulation of gallic acid and evaluation of its cytotoxicity and antioxidant activity.
abstract: Gallic acid is an important polyphenol compound presenting various biological activities. The objective of this study was to prepare, characterize and evaluate poly(lactic-co-glycolic acid) (PLGA) nanoparticles coated or not with polysorbate 80 (PS80) containing gallic acid. Nanoparticles coated or not with PS80 were produced by emulsion solvent evaporation method and presented a mean size of around 225 nm, gallic acid encapsulation efficiency of around 26% and zeta potential of -22 mV. Nanoparticle formulations were stable during storage, except nanoparticles coated with PS80 stored at room temperature. In vitro release profile demonstrated a quite sustained gallic acid release from nanoparticles and PS80-coating decreased drug release. Cytotoxicity over red blood cells was assessed and gallic acid-loaded PLGA nanoparticles at all analyzed concentrations demonstrated lack of hemolysis, while PS80-nanoparticles containing gallic acid were cytotoxic only in higher concentrations. Antioxidant potential of nanoparticles containing gallic acid was assessed and PLGA uncoated nanoparticles presented greater efficacy than PS80-coated PLGA nanoparticles.
authors.affiliation: Department of Pharmacy, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá 03, 85040-080, Guarapuava, PR, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: de Cristo Soares Alves
authors.affiliation: Department of Pharmacy, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá 03, 85040-080, Guarapuava, PR, Brazil.
authors.forename: Rubiana Mara
authors.initials: RM
authors.lastname: Mainardes
authors.affiliation: Department of Pharmacy, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá 03, 85040-080, Guarapuava, PR, Brazil. Electronic address: najehunicentro@hotmail.com.
authors.forename: Najeh Maissar
authors.initials: NM
authors.lastname: Khalil
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005707:Gallic Acid
keywords: Antioxidant; Gallic acid; Hemolysis; Nanoparticles