title: Effects of sodium nitroprusside on mouse erythrocyte catalase activity and malondialdehyde status.
abstract: There is controversy about the anti- or pro-oxidative effects of the nitric oxide (NO)-donor sodium nitroprusside (SNP). Hence, the activity of the antioxidant enzyme catalase (CAT) and the status of malondialdehyde (MDA) were investigated after a 2.5 mg/kg dose of SNP had been i.p. administered to different and comparable groups of mice (n = 48). The drug was administered at two different circadian times (1 and 13 h after light onset [HALO]). There were, irrespectively of sampling time, no significant differences in the means of CAT activity and MDA status between control and SNP-treated groups, no matter the treatment time. However, CAT activity was significantly (Student's t-test, p < 0.001) increased 1 h following SNP administration at 1 HALO, whereas the significant (p < 0.001) increase in the enzyme activity was found only 3 h after injection at 13 HALO. The drug dosing either at 1 or 13 HALO resulted in no significant differences of MDA status between control and treated groups regardless to the sampling time. Two-way analysis of variance (ANOVA) detected a significant (F0.05(7,88)= 5.3; p < 0.0006) interaction between sampling time and treatment in mice injected at 1 HALO, suggesting the influence of treatment on sampling-time-related changes in CAT activity. However, ANOVA validated no interaction between the two factors in mice treated at 13 HALO, illustrating that the sampling-time differences in enzyme activity were greater. Furthermore, two-way ANOVA revealed no interaction in the variation of MDA status in animals treated either at 1 or 13 HALO. This study indicates that SNP significantly affected the anti-oxidant system.
authors.affiliation: a Département De Biologie, Faculté Des Sciences Et Techniques De Maradi , UMR Biosurveillance Et Toxicologie Environnementale , Maradi , Niger .|c Circadian Rhythm Laboratory , Boise State University , Boise , ID , USA .
authors.forename: Mamane
authors.initials: M
authors.lastname: Sani
authors.affiliation: b Département Des Sciences De La Vie , UR Ethnobotanie Et Stress Oxydant , Zarzouna , Tunisia .
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
authors.affiliation: c Circadian Rhythm Laboratory , Boise State University , Boise , ID , USA .
authors.forename: Roberto
authors.initials: R
authors.lastname: Refinetti
authors.affiliation: d National Dairy Research Institute , Kalyani , West Bengal , India .
authors.forename: Mohan
authors.initials: M
authors.lastname: Mondal
authors.affiliation: b Département Des Sciences De La Vie , UR Ethnobotanie Et Stress Oxydant , Zarzouna , Tunisia .
authors.forename: Néziha
authors.initials: N
authors.lastname: Ghanem-Boughanmi
authors.affiliation: e Laboratoire De Pharmacologie, Faculté De Médecine , Monastir , Tunisia , and.
authors.forename: Naceur A
authors.initials: NA
authors.lastname: Boughattas
authors.affiliation: f Laboratoire De Biosurveillance De L'environnement, Faculté Des Sciences De Bizerte , Zarzouna , Tunisia.
authors.forename: Mossadok
authors.initials: M
authors.lastname: Ben-Attia
mesh_terms: D000818:Animals; D002374:Catalase / Q000097:blood / Q000378:metabolism*; D057906:Circadian Clocks; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D007274:Injections, Intraperitoneal; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D051379:Mice; D020030:Nitric Oxide Donors / Q000008:administration & dosage / Q000494:pharmacology*; D009599:Nitroprusside / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D020030:Nitric Oxide Donors; D009599:Nitroprusside; D008315:Malondialdehyde; D002374:Catalase
keywords: Catalase; erythrocyte; malondialdehyde; mouse; sodium nitroprusside