title: High expression levels of MAGE-A9 are correlated with unfavorable survival in lung adenocarcinoma.
abstract: A variety of melanoma-associated antigen-A (MAGE-A) protein are commonly detected in lung cancers. Their biological function is not well characterized but may involve cell cycle progression and the regulation of apoptosis. We hypothesized that MAGE-A9 is involved in the regulation of apoptosis. To test this hypothesis, we evaluated MAGE-A9 protein expression by immunohistochemical staining and we assessed the relationship between the expression of MAGE-A9 and clinical pathological parameters. In addition, we investigated the effect of MAGE-A9 down-regulation in lung adenocarcinoma. The results showed that a high expression level of MAGE-A9 protein in lung adenocarcinoma tumor cells was related to larger tumor diameter (P = 0.013) and poor differentiation (P = 0.029). Cox regression analysis revealed that the expression of MAGE-A9 in lung adenocarcinoma tumor cells (P < 0.001) is an independent prognostic factor in five-year survival rates. NSCLC cells with silenced MAGE-A9 had decreased cell proliferation, migration and invasion in cell culture compared to corresponding control cells. The NSCLC cells showing down-regulated MAGE-A9 induced the expression of apoptosis-associated proteins. In addition, MAGE-A9 was associated with resistance to conventional chemotherapeutic agents. Our findings provide evidence that MAGE-A9 could be a potential therapeutic target in NSCLC.
authors.affiliation: Department of Chemotherapy, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Department of Respiratory, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Chemotherapy, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Siya
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Chemotherapy, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Mao
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000401:mortality* / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000378:metabolism*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000401:mortality* / Q000556:secondary; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000188:drug therapy / Q000401:mortality* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C520253:MAGEA9 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger
keywords: MAGE-A9; apoptosis; immunohistochemistry; lung adenocarcinoma; prognosis