title: Advances on the molecular characterization, clinical relevance, and detection methods of Gadiform parvalbumin allergens.
abstract: Gadiform order includes several fish families, from which Gadidae and Merlucciidae are part of, comprising the most commercially important and highly appreciated fish species, such as cod, pollock, haddock, and hake. Parvalbumins, classified as calcium-binding proteins, are considered the main components involved in the majority of fish allergies. Nine and thirteen parvalbumins were identified in different fish species from Gadidae and Merlucciidae families, respectively. This review intends to describe their molecular characterization and the clinical relevance, as well as the prevalence of fish allergy. In addition, the main protein- and DNA-based methods to detect fish allergens are fully reviewed owing to their importance in the safeguard of sensitized/allergic individuals.
authors.affiliation: a REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: Telmo J R
authors.initials: TJR
authors.lastname: Fernandes
authors.affiliation: a REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Costa
authors.affiliation: b Serviço de Imunoalergologia, Centro Hospitalar e Universitário de Coimbra , Coimbra , Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Carrapatoso
authors.affiliation: a REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: Maria Beatriz P P
authors.initials: MBPP
authors.lastname: Oliveira
authors.affiliation: a REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Mafra
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D005399:Fishes / Q000276:immunology*; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D010320:Parvalbumins / Q000276:immunology*; D017747:Seafood
chemical_list: D000485:Allergens; D010320:Parvalbumins
keywords: DNA-based methods; Food allergen; Gadidae; Merlucciidae; fish allergy; molecular characterization; parvalbumin detection; prevalence; protein-based methods