title: Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers.
abstract: A study was conducted to assess the genetic diversity among Simmental Cross cattle in West Sumatra using microsatellite DNA markers. A total of 176 individual cattle blood samples was used for obtaining DNA samples. Twelve primers of microsatellite loci as recommended by FAO were used to identify the genetic diversity of the Simmental Cross cattle population. Multiplex DNA fragment analysis method was used for allele identification. All the microsatellite loci in this study were highly polymorphic and all of the identified alleles were able to classify the cattle population into several groups based on their genetic distance. The heterozygosity values of microsatellite loci in this study ranged from 0.556 to 0.782. The polymorphism information content (PIC) value of the 12 observed loci is high (PIC>0.5). The highest PIC value in the Simmental cattle population was 0.893 (locus TGLA53), while the lowest value was 0.529 (locus BM1818). Based on the genetic distance value, the subpopulation of the Simmental Cross-Agam and the Simmental Cross-Limapuluh Kota was exceptionally close to the Simmental Purebred thus indicating that a grading-up process has taken place with the Simmental Purebred. In view of the advantages possessed by the Simmental Cross cattle and the evaluation of the genetic diversity results, a number of subpopulations in this study can be considered as the initial (base) population for the Simmental Cross cattle breeding programs in West Sumatra, Indonesia.
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Paskah Partogi
authors.initials: PP
authors.lastname: Agung
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Ferdy
authors.initials: F
authors.lastname: Saputra
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Wike Andre
authors.initials: WA
authors.lastname: Septian
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.lastname: Lusiana
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Moch Syamsul Arifin
authors.initials: MS
authors.lastname: Zein
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Sri
authors.initials: S
authors.lastname: Sulandari
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Saiful
authors.initials: S
authors.lastname: Anwar
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Ari Sulistyo
authors.initials: AS
authors.lastname: Wulandari
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Syahruddin
authors.initials: S
authors.lastname: Said
authors.affiliation: Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, Indonesia.
authors.forename: Baharuddin
authors.initials: B
authors.lastname: Tappa
keywords: Genetic Diversity; Microsatellite; Simmental; West Sumatra