title: Evaluation of dentin tubule occlusion after laser irradiation and desensitizing agent application.
abstract: OBJECTIVE
  To evaluate the effects of lasers (Nd:YAG and Er:YAG) and of topical desensitizing agents on dentin tubule occlusion by measuring real-time dentin fluid flow (DFF).
  
  
  METHODS
  32 molars were prepared with V-shape cavity at the cervical area, acid-etched, water rinsed, blotted dry, and treated with (1) Nd:YAG laser; (2) Er:YAG laser; (3) SuperSeal, a desensitizing agent; (4) ClinproXT, a resin-modified glass-ionomer (RMGI) varnish (n = 8 each). A real-time fluid flow measuring instrument (nano-Flow) was used to measure the DFF throughout the procedures. The DFF rates before and after the treatment were compared. Moreover, the surface topography of dentin tubules after each desensitizing method was examined using SEM.
  
  
  RESULTS
  DFF varied among the groups. The DFF rate was significantly reduced after laser irradiation/application of the desensitizing agents (P < 0.05). ClinproXT showed the greatest reduction of DFF rate (71.9%), followed by the SuperSeal (34.8%) and laser groups (P< 0.05). However, there was no significant difference between the Nd:YAG (24.1%) and Er:YAG (20.6%) groups (P > 0.05). In SEM images, narrowed dentin tubules were observed in both lased groups and SuperSeal group. In the ClinproXT group, the occluded dentin tubules by the RMGI covering were observed.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Kim
authors.forename: Ryan Jin-Young
authors.initials: RJ
authors.lastname: Kim
authors.forename: Woo-Cheol
authors.initials: WC
authors.lastname: Lee
authors.forename: In-Bog
authors.initials: IB
authors.lastname: Lee
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D003737:Dental Cavity Preparation / Q000145:classification; D003804:Dentin / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure*; D057133:Dentin Desensitizing Agents / Q000627:therapeutic use*; D003807:Dentin Sensitivity / Q000503:physiopathology; D019234:Dentinal Fluid / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D005899:Glass Ionomer Cements / Q000737:chemistry; D006801:Humans; D057446:Hydrodynamics; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D019815:Oxalic Acid / Q000627:therapeutic use; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry; D019237:Tooth Cervix / Q000648:ultrastructure
chemical_list: D057133:Dentin Desensitizing Agents; D005899:Glass Ionomer Cements; D019279:Resin Cements; C474198:SuperSeal; D019815:Oxalic Acid