title: Anthocyanin Accumulation, Antioxidant Ability and Stability, and a Transcriptional Analysis of Anthocyanin Biosynthesis in Purple Heading Chinese Cabbage (Brassica rapa L. ssp. pekinensis).
abstract: Heading Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a significant dietary vegetable for its edible heading leaves in Asia countries. The new purple anthocyanin-rich pure line (11S91) was successfully bred, and the anthocyanins were mainly distributed in 2-3 cell layers beneath the leaf epidermis, whereas siliques and stems accumulated only a cell layer of anthocyanins. The anthocyanins of 11S91 were more stable at pHs below 3.0 and temperatures below 45 °C. The total antioxidant ability was highly positive correlated with the anthocyanin content in 11S91. Thirty-two anthocyanins were separated and identified, and 70% of them were glycosylated and acylated cyanidins. The four major anthocyanins present were cyanidin-3-sophoroside(p-coumaroyl)-5-glucoside(malonyl), cyanidin-3-sophoroside(ferulyl)-5-glucoside(malonyl), cyanidin-3-sophoroside(sinapyl-p-coumaroyl)-5-glucoside(malonyl), and cyanidin-3-sophoroside-(sinapyl-ferulyl)-5-glucoside(malonyl). According to the expression of biosynthetic genes and the component profile of anthocyanins in 11S91 and its parents, regulatory genes BrMYB2 and BrTT8 probably activate the anthocyanin biosynthesis but other factors may govern the primary anthocyanins and the distribution.
authors.affiliation: College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University , 3 Taicheng Road, Yangling 712100, Shaanxi, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: He
authors.affiliation: College of Plant Protection, Northwest A&F University , 3 Taicheng Road, Yangling 712100, Shaanxi, People's Republic of China.
authors.forename: Zhanfeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University , 3 Taicheng Road, Yangling 712100, Shaanxi, People's Republic of China.
authors.forename: Lugang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001937:Brassica / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003116:Color; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010940:Plant Proteins
keywords: Chinese cabbage; LC-MS/MS; anatomical feature; anthocyanin; antioxidant ability; gene expression; purple heading