title: [Effects of blueberry on apoptosis and expression of Bcl-2 and Bax in HSC-T6].
abstract: OBJECTIVE
  To investigate the effects of blueberry on the apoptosis, expression of Bcl-2 and Bax in rat hepatic stellate cell (HSC-T6).
  
  
  METHODS
  10% blueberry serum at low, middle and high dose, 10% Fu-Fang-Bie-Jia-Ruan-Gan tablet serum and 10% saline serum were prepared by method of serum pharmacology. Subcultured HSC-T6 was divided into saline serum control group, blueberry serum at low, middle, high dose and Fu-Fang-Bie-Jia-Ruan-Gan tablet serum group, and then was respectively incubated at different dose of 10% blueberry serum, 10% Fu-Fang-Bie-Jia-Ruan-Gan tablet serum and 10% saline serum for 72 hours.Apoptosis of HSC-T6 was detected using flow cytometry with annexin V FITC/PI double staining. The expression of Bcl-2 and Bax in HSC-T6 were examined using immunocytochemistry and Western blotting, respectively.
  
  
  RESULTS
  There was no significant difference for HSC-T6 Bax protein expression in the low, middle and high dose blueberry serum groups, compared with saline serum control group, respectively.In the high-dose blueberry serum group HSC-T6 early and total apoptosis rate increased significantly compared with the saline serum control group (5.55% ± 0.98% vs 2.53% ± 0.46%, 7.01% ± 1.05% vs 2.96% ± 0.81%, both P<0.05); Bcl-2 protein expression was significantly decreased (A value, 82 ± 35 vs 51 ± 13, P<0.05); Bcl-2/Bax ratio was significantly decreased (0.26 ± 0.02 vs 0.46 ± 0.03, P<0.05); HSC-T6 early and total apoptosis rate, Bcl-2 expression and Bcl-2/Bax ratio in the low and the middle dose blueberry serum group showed no significant difference with the saline serum control group.
  
  
  CONCLUSIONS
  Blueberry can induce HSC-T6 apoptosis by down-regulating Bcl-2 expression and decreasing the ratio of Bcl-2/Bax in HSC-T6 cells, so it may have potential interference effects on hepatic fibrosis.
authors.affiliation: Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China; Email: chengml@21cn.com.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Cheng
authors.affiliation: Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Demeng
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D029798:Blueberry Plants; D002460:Cell Line; D055166:Hepatic Stellate Cells; D008103:Liver Cirrhosis; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D044967:Serum; D051028:bcl-2-Associated X Protein
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein