title: Comparative expression profiling of genes involved in primary metabolism in high-yield and wild-type strains of Acremonium chrysogenum.
abstract: Cephalosporin C (CPC) productivity of Acremonium chrysogenum has been improved significantly through classical strain improvement programs. Here, we used transcription and metabolite profiling to address mechanisms underlying CPC production in a high yield (HY) strain. Transcription and metabolite profiling indicated that enzymes involved in amino acid production are higher in abundance in the HY strain. Moreover, results indicate a higher flow of precursors from the glycolysis and gluconeogenesis pathways to serine synthesis at the late stage of fermentation in the HY strain. In addition, less pyruvate would enter the TCA cycle thus favoring valine synthesis. Amino acid production would also benefit from a more active pentose phosphate pathway and γ-amino butyric acid shunt both generating NADPH. Moreover the glyoxylate pathway seems to be more active in the HY strain. These results may provide new leads for CPC strain improvement in industry.
authors.affiliation: Shanghai Institute of Pharmaceutical Industry, 1320 Beijing Road (W), Shanghai, 200040, China.|China State Institute of Pharmaceutical Industry, Zhangjiang Institute, 285 Gebaini Road, Shanghai, 201203, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Han
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, 285 Gebaini Road, Shanghai, 201203, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, 285 Gebaini Road, Shanghai, 201203, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.affiliation: Shanghai Institute of Pharmaceutical Industry, 1320 Beijing Road (W), Shanghai, 200040, China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Zhu
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, 285 Gebaini Road, Shanghai, 201203, China. bebydou@hotmail.com.
authors.forename: Youjia
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000164:Acremonium / Q000235:genetics* / Q000378:metabolism*; D001481:Basal Metabolism / Q000235:genetics*; D002511:Cephalosporins / Q000096:biosynthesis; D002952:Citric Acid Cycle; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005943:Gluconeogenesis; D006019:Glycolysis; D006038:Glyoxylates / Q000378:metabolism; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D019802:Succinic Acid / Q000378:metabolism; D059467:Transcriptome
chemical_list: D002511:Cephalosporins; D006038:Glyoxylates; C025163:cephalosporin C; D019802:Succinic Acid; C031150:glyoxylic acid
keywords: Acremonium chrysogenum; CPC overproduction; Gene expression; Primary metabolism