title: microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4.
abstract: BACKGROUND
  Dysregulation of microRNA-150 (miR-150) is commonly observed in solid tumor and has been reported to be involved in multiple important biological processes, such as cell proliferation, apoptosis, and metastasis. Elevated miR-150 level was also detected in cervical carcinoma, whereas its function in cancer progression has not been studied yet.
  
  
  METHODS
  The expression of miRNA-150 in cervical carcinoma was compared with normal cervical tissue and using qRT-PCR. The effects of miR-150 on cell cycle and apoptosis, as well as the expression of cycle- and apoptosis-related genes, were determined using flow cytometry, TUNEL assay, qRT-PCR, and Western blot, respectively. The direct target of miR-150 was confirmed using 3' untranslated region (UTR) luciferase reporter assay.
  
  
  RESULTS
  miR-150 promotes cervical cancer cell survival and growth, while the inhibition of miR-150 suppresses these actions. miR-150 also induced the cell cycle progression from G1/G0 to S phase, resulting in an enhancement of growth. Several cell cycle- and apoptosis-related genes, CyclinD1, p27, BIM, and FASL were modulated by miR-150. Moreover, miR-150 directly reduced the expression of FOXO4, which regulates the expression of CyclinD1, p27, BIM, and FASL, by targeting its 3' UTR.
  
  
  CONCLUSIONS
  Taken together, our data demonstrated that elevated miR-150 targets FOXO4 expression and therefore regulates multiple genes expression, resulting in cervical cancer cell growth and survival.
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Linjiang Road 76, Chongqing, 400010, People's Republic of China. junlichongqing@163.com.|Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. junlichongqing@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Linjiang Road 76, Chongqing, 400010, People's Republic of China. linahu3321@sina.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. tianchao163@hotmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Tian
authors.affiliation: Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. fenglu1587@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. drjiawu@tom.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. lililiu122@sina.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D000072224:Bcl-2-Like Protein 11; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics*; D019938:Cyclin D1 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D008565:Membrane Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D000072224:Bcl-2-Like Protein 11; D018797:Cell Cycle Proteins; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D053222:Fas Ligand Protein; D051858:Forkhead Transcription Factors; D008565:Membrane Proteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; C000606775:BCL2L11 protein, human; C530104:CCND1 protein, human; C436291:CDKN1B protein, human; C505661:FASLG protein, human; C108803:FOXO4 protein, human; C533097:MIR150, human