title: Influence of encapsulated heat shock protein HSP70 on the basic functional properties of blood phagocytes.
abstract: Microencapsulated heat shock proteins HSP 70 were studied in terms of their effects on neutrophil apoptosis, production of reactive oxygen species, and secretion of TNF-α by human neurtrophils and monocytes. Encapsulated HSP70 inhibited neutrophil apoptosis by 65% as compared to the effect of nonencapsulated HSP70; TNF-α production by the promonocytic THP-1 cells was similarly inhibited by the non-encapsulated and encapsulated HSP70. Thus, the polyelectrolyte micromolecules can be used as containers for effective delivery of HSP70 up to neutrophils and monocytes to correct the innate immunity functions.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia. o.y.kochetkova@gmail.com.|Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia. o.y.kochetkova@gmail.com.
authors.forename: O Yu
authors.initials: OY
authors.lastname: Kochetkova
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Yurinskaya
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.|Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: M B
authors.initials: MB
authors.lastname: Evgen'ev
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: O G
authors.initials: OG
authors.lastname: Zatsepina
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
authors.forename: L I
authors.initials: LI
authors.lastname: Shabarchina
authors.affiliation: Hospital of the Pushchino Scientific Center, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Suslikov
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Tikhonenko
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Vinokurov
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D004339:Drug Compounding; D016503:Drug Delivery Systems; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D010586:Phagocytes / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha