title: Genome-wide analysis and expression profiling of the phospholipase D gene family in Gossypium arboreum.
abstract: The plant phospholipase D (PLD) plays versatile functions in multiple aspects of plant growth, development, and stress responses. However, until now, our knowledge concerning the PLD gene family members and their expression patterns in cotton has been limited. In this study, we performed for the first time the genome-wide analysis and expression profiling of PLD gene family in Gossypium arboretum, and finally, a total of 19 non-redundant PLD genes (GaPLDs) were identified. Based on the phylogenetic analysis, they were divided into six well-supported clades (α, β/γ, δ, ε, ζ and φ). Most of the GaPLD genes within the same clade showed the similar exon-intron organization and highly conserved motif structures. Additionally, the chromosomal distribution pattern revealed that GaPLD genes were unevenly distributed across 10 of the 13 cotton chromosomes. Segmental duplication is the major contributor to the expansion of GaPLD gene family and estimated to have occurred from 19.61 to 20.44 million years ago when a recent large-scale genome duplication occurred in cotton. Moreover, the expression profiling provides the functional divergence of GaPLD genes in cotton and provides some new light on the molecular mechanisms of GaPLDα1 and GaPLDδ2 in fiber development.
authors.affiliation: Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Tang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Dong
authors.affiliation: Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China. liujy@mail.tsinghua.edu.cn.
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Liu
mesh_terms: D005091:Exons; D020869:Gene Expression Profiling; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D007438:Introns; D005810:Multigene Family; D010739:Phospholipase D / Q000235:genetics*; D010802:Phylogeny
chemical_list: D010739:Phospholipase D
keywords: Gossypium arboreum; expression patterns; fiber development; gene family; phospholipase D