title: Downregulation of microRNA-206 suppresses clear cell renal carcinoma proliferation and invasion by targeting vascular endothelial growth factor A.
abstract: MicroRNA-206 (miR-206) has been discovered to have anticancer properties in different tissues. However, the role of miR-206 on renal carcinoma is still ambiguous. In the present study, we investigated the role of miR-206 on the development of renal carcinoma. The results indicated that miR-206 was significantly downregulated in 69 clear cell renal carcinoma (ccRCC) tissues and low-level of miR-206 related to shorter metastasis-free survival time for patients with ccRCC. The results indicated that vascular endothelial growth factor A (VEGFA) was a direct target of miR-206 in renal cancer cells. Further studies revealed that upregulation of miR-206 inhibited renal cancer cell proliferation, invasion and migration, suggesting that miR-206 functioned as a tumor suppressor. RNA interference targeting VEGFA mRNA could mimic the upregulation of miR-206 functions, and also suppressed tumor formation in vivo in nude mice. These results suggest that miR-206 plays an important role in ccRCC tumorigenesis by targeting VEGFA.
authors.affiliation: Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A