title: Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells.
abstract: Light-emitting diodes (LED) have recently been introduced as a potential factor for proliferation of various cell types in vitro. Nowadays, stem cells are widely used in regenerative medicine. Human umbilical cord matrix-derived mesenchymal (hUCM) cells can be more easily isolated and cultured than adult mesenchymal stem cells. The aim of this study was to evaluate the effect of red and green lights produced by LED on the proliferation of hUCM cells. hUCM cells were isolated from the umbilical cord, and light irradiation was applied at radiation energies of 0.318, 0.636, 0.954, 1.59, 3.18, 6.36, 9.54, and 12.72 J/cm(2). Irradiation of the hUCM cells shows a significant (p < 0.05) increase in cell number as compared to controls after 40 h. In addition, cell proliferation on days 7, 14, and 21 in irradiated groups were significantly (p < 0.001) higher than that in the non-irradiated groups. The present study clearly demonstrates the ability of red and green lights irradiation to promote proliferation of hUCM cells in vitro. The energy applied to the cells through LED irradiation is an effective factor with paradoxical alterations. Green light inserted a much profound effect at special dosages than red light.
authors.affiliation: Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Samereh
authors.initials: S
authors.lastname: Dehghani Soltani
authors.affiliation: Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Babaee
authors.affiliation: Afzal Research Institute, Kerman, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shojaei
authors.affiliation: Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Parvin
authors.initials: P
authors.lastname: Salehinejad
authors.affiliation: Department of Anatomy, Jiroft University of Medical Sciences, Kerman, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Seyedi
authors.affiliation: Semiconductors Group, Photonics Research Center, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Mahshid
authors.initials: M
authors.lastname: JalalKamali
authors.affiliation: Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. nnematollahi@kmu.ac.ir.|Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. nnematollahi@kmu.ac.ir.
authors.forename: Seyed Noureddin
authors.initials: SN
authors.lastname: Nematollahi-Mahani
mesh_terms: D049109:Cell Proliferation / Q000528:radiation effects; D003116:Color; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008027:Light; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors; D014470:Umbilical Cord / Q000166:cytology*
keywords: Cell proliferation; Light-emitting diode; Red and green lights; Umbilical cord matrix-derived cells