title: Isolation and Characterization of a Novel Bat Coronavirus Closely Related to the Direct Progenitor of Severe Acute Respiratory Syndrome Coronavirus.
abstract: We report the isolation and characterization of a novel bat coronavirus which is much closer to the severe acute respiratory syndrome coronavirus (SARS-CoV) in genomic sequence than others previously reported, particularly in its S gene. Cell entry and susceptibility studies indicated that this virus can use ACE2 as a receptor and infect animal and human cell lines. Our results provide further evidence of the bat origin of the SARS-CoV and highlight the likelihood of future bat coronavirus emergence in humans.
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xing-Lou
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Ben
authors.initials: B
authors.lastname: Hu
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Mei-Niang
authors.initials: MN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wu
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Ge
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China.
authors.forename: Yun-Zhi
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: EcoHealth Alliance, New York, New York, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Daszak
authors.affiliation: Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore, Singapore.
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China zlshi@wh.iov.cn.
authors.forename: Zheng-Li
authors.identifier: http://orcid.org/0000-0001-8089-163X
authors.initials: ZL
authors.lastname: Shi
mesh_terms: D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D002460:Cell Line; D002685:Chiroptera / Q000821:virology*; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011991:Receptors, Virus / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics; D053585:Virus Attachment
chemical_list: D012367:RNA, Viral; D011991:Receptors, Virus; D064370:Spike Glycoprotein, Coronavirus; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2