title: Morphological and molecular characterization of Oestromyia leporina (Pallas, 1778) (Diptera: Hypodermatinae) from wild plateau pikas(Ochotona curzoniae) in Qinghai province, China.
abstract: The objective of this article was to investigate the morphological and molecular characterization of Oestromyia leporina (Pallas, 1778) from wild plateau pikas (Ochotona curzoniae) in Qinghai province, China. The third-stage larvae of O. leporina were examined by scanning electron microscopy revealing morphology characteristics of the spines on the cephalic, the thoracic segments, the abdominal segments and the spiracular plates. The coding regions of 25 cytochrome oxidase I (COI) genes of O. leporina were investigated. Eighty-one variable sites and 21 haplotypes were identified and the nucleotide and haplotype diversities were 0.04456 and 0.9767, respectively, indicating a rich genetic diversity in O. leporina. Phylogenetic analysis utilizing sequences of COI revealed two distinct lineages. These findings revealed ultrastructure and molecular characterization among the O. leporina from plateau pikas in Qinghai province, China and had implications for studying morphological identification, molecular epidemiology and population genetics of O. leporina.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China; Academy of Animal and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Academy of Animal and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Academy of Animal and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Duo
authors.affiliation: Academy of Animal and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Dang
authors.affiliation: Academy of Animal and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Shen
authors.affiliation: Academy of Animal and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Peng
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China. Electronic address: Zhangym@nwsuaf.edu.cn.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D004175:Diptera / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure*; D004478:Ectoparasitic Infestations / Q000469:parasitology / Q000662:veterinary*; D006239:Haplotypes; D007793:Lagomorpha / Q000469:parasitology*; D007814:Larva / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D011110:Polymorphism, Genetic; D012337:RNA, Ribosomal, 18S / Q000235:genetics*
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: Cytochrome oxidase I; Genetic diversity; Mitochondrial DNA; Ochotona curzoniae; Oestromyia leporina; Scanning electron microscopy