title: Isopropanol biodegradation by immobilized Paracoccus denitrificans in a three-phase fluidized bed reactor.
abstract: A three-phase bed bioreactor including a mix of immobilized microbes was used to degrade isopropanol (IPA). The immobilization method was studied and cells immobilized with calcium alginate, polyvinyl alcohol, activated carbon, and SiO2were demonstrated to be the best immobilization method for the degradation of 90% of 2 g/L IPA in just 4 days, 1 day earlier than with free cells. Acetone was monitored as an indicator of microbial IPA utilization as the major intermediate of aerobic IPA biodegradation. The bioreactor was operated at hydraulic retention time (HRT) values of 32, 24, 16, 12, and 10 hr, which correspond to membrane fluxes of 0.03, 0.04, 0.06, 0.08, and 0.10 L/m2/hr, respectively. The chemical oxygen demand (COD) removal efficiencies were maintained at 98.0, 97.8, 89.1, 80.6, and 71.1% at a HRT of 32, 24, 16, 12, and 10 hr, respectively, while the IPA degradations were 98.6, 98.3, 90.3, 81.6, and 73.3%, respectively. With a comprehensive consideration of COD removal and economy, the optimal HRT was 24 hr. The results demonstrate the potential of immobilized mixed bacterial consortium in a three-phase fluidized bed reactor system for the aerobic treatment of wastewater containing IPA.
authors.affiliation: a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Geng
authors.affiliation: a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Deng
authors.affiliation: a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
authors.forename: Feilong
authors.initials: F
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.affiliation: a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
authors.forename: Taiping
authors.initials: T
authors.lastname: Hou
authors.affiliation: a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tao
mesh_terms: D019840:2-Propanol / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D018914:Cells, Immobilized / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D004867:Equipment Design; D010231:Paracoccus denitrificans / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D019840:2-Propanol
keywords: Biodegradation; Paracoccus denitrificans; hydraulic retention time; immobilization; isopropanol; three-phase fluidized bed