title: Patient-Specific Therapy via Cell-Reprogramming Technology: a Curative Potential for Patients with Diabetes.
abstract: Gene therapeutics provides great opportunities for curing diabetes. Numerous attempts have been made to establish a safe and high-efficiency gene delivery strategy, but all of them are unsuccessful. To achieve an ideal transfection, a novel gene delivery strategy was presented in this research. The novel system proposed was transfection mediated by the combination of ultrasound with microbubbles and cross-linked polyethylenimines (PEIs). Ultrasound with microbubbles enhances the permeability of target cells; moreover, cross-linked PEIs enabled DNA to escape from endosomes into the cytoplasm. If the proposed method is feasible and effective, the endogenous secretion system of insulin would be re-established in patients with diabetes.
authors.affiliation: Department of Endocrinology, Nanhai Hospital, Southern Medical University, No. 40, Fo Ping Road, Foshan, 528200, China. luohaizhao@126.com.
authors.forename: Haizhao
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University, No. 253, Gong Ye Road, Guangzhou, 510282, China. 659735943@qq.com.
authors.forename: Xianbao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Guangzhou Red Cross Hospital, No. 396, Tongfu Zhong Road, Guangzhou, 510220, China. 416979293@qq.com.
authors.forename: Ruyi
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Nanhai Hospital, Southern Medical University, No. 40, Fo Ping Road, Foshan, 528200, China. 18076487@qq.com.
authors.forename: Youping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Nanhai Hospital, Southern Medical University, No. 40, Fo Ping Road, Foshan, 528200, China. 790806200@qq.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Endocrinology, Nanhai Hospital, Southern Medical University, No. 40, Fo Ping Road, Foshan, 528200, China. 592624843@qq.com.
authors.forename: Huixian
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, No. 253, Gong Ye Road, Guangzhou, 510282, China. rubychq@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
keywords: Cross-linked polyethylenimines; Gene therapeutics; Microbubbles; Ultrasound