title: Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity.
abstract: Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Superior, Lurie 9-280, Chicago, IL, 60611, USA.
authors.forename: João I
authors.initials: JI
authors.lastname: Mamede
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Superior, Lurie 9-280, Chicago, IL, 60611, USA. thope@northwestern.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Hope
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D015658:HIV Infections / Q000473:pathology* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008853:Microscopy / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D012984:Software; D013194:Staining and Labeling / Q000379:methods; D057646:Virus Uncoating
keywords: HIV early steps of infection; HIV fusion; HIV uncoating; HIV viral core; Live cell imaging; Wide-field microscopy