title: 14-3-3β Promotes Migration and Invasion of Human Hepatocellular Carcinoma Cells by Modulating Expression of MMP2 and MMP9 through PI3K/Akt/NF-κB Pathway.
abstract: 14-3-3β has been demonstrated to possess the oncogenic potential, and its increased expression has been detected in multiple types of carcinomas. However, majority of previous studies focused on the role of 14-3-3β in tumor cell proliferation and apoptosis, leaving much to be elucidated about its function in tumor cell invasion and metastasis. Hence, the present study aimed to investigate the role of 14-3-3β in the invasion of hepatocellular carcinoma (HCC) cells and the implications in the prognosis of HCC patients. We first examined the expression of 14-3-3β in the primary tumors of HCC patients with or without portal vein tumor thrombus (PVTT), and found that 14-3-3β expression was higher in the primary tumors with PVTT, and the level was even higher in the PVTTs. Kaplan-Meier curves and multivariate analysis revealed that high expression of 14-3-3β was associated with overall survival (OS) and time to recurrence (TTR) of HCC patients. In addition, ectopic expression of 14-3-3β in HCC cell lines led to enhanced migration ability and invasiveness, as well as up-regulation of matrix metalloproteinase 2 and 9, which could be suppressed by inhibiting the activation of Akt and nuclear factor-κB (NF-κB) signaling. Furthermore, we identified a correlated elevation of 14-3-3β and p-Akt in the primary tumors of HCC patients, and showed that a combinatory detection of 14-3-3β and p-Akt could better predict post-surgical outcome of HCC patients.
authors.affiliation: Department of Hepatobiliary Surgery, The General Hospital of Shenyang Military Area Command, Shenyang, People's Republic of China.|Post-doctoral Station, The General Hospital of Shenyang Military Area Command, Shenyang, People's Republic of China.
authors.forename: Yufu
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Hepatobiliary Surgery, The General Hospital of Shenyang Military Area Command, Shenyang, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Lv
authors.affiliation: Department of Hepatobiliary Surgery, The General Hospital of Shenyang Military Area Command, Shenyang, People's Republic of China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Hepatobiliary Surgery, The General Hospital of Shenyang Military Area Command, Shenyang, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Hepatobiliary Surgery, The General Hospital of Shenyang Military Area Command, Shenyang, People's Republic of China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhou
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011169:Portal Vein / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D016019:Survival Analysis; D020246:Venous Thrombosis / Q000473:pathology
chemical_list: D048948:14-3-3 Proteins; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9