title: Tumor Suppressor Role of miR-363-3p in Gastric Cancer.
abstract: BACKGROUND Gastric cancer (GC) is the most common cancer in the world. Despite the advancement of the treatment of GC, the 5-year overall survival rate is still very low. MicroRNAs (miRNAs) play important roles in the pathogenesis of GC. A recent study suggested that miR-363-3p plays a role in the development of GC. However, the function of miR-363-3p in GC is not fully understood. MATERIAL AND METHODS The network of NOTCH1 and the involved molecules was constructed by use of Cytoscape software. MiR-363-3p levels in GC tissues and cells were tested by qRT-PCR. Cells were miR-363-3p mimics or anti-miR-363-3p transfected by Lipofectamine. Bioinformatics algorithms from TargetScanHuman were used to predict the target genes of miR-363-3p. The NOTCH1 protein level was tested by Western blot. The interaction between miR-363-3p and NOTCH1 was confirmed by dual luciferase assays. RESULTS MiR-363-3p showed low levels in GC tissues and cells. Enforced expression of miR-363-3p inhibited cell growth and migration of GC cells and vice versa. NOTCH1 is the targeted gene of miR-363-3p. CONCLUSIONS MiR-363-3p plays a tumor suppressor role in GC.
authors.affiliation: Division of Intestinal Surgery, Department of Abdominal Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan, China (mainland).
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Abdominal Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan, China (mainland).
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Abdominal Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan, China (mainland).
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Abdominal Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan, China (mainland).
authors.forename: Haiyang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Abdominal Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan, China (mainland).
authors.forename: Yangchun
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D002453:Cell Cycle / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C574861:MIRN363 microRNA, human; D035683:MicroRNAs; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1