title: Designing a reusable system based on nanodiamonds for biochemical determination of urea.
abstract: A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored product after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro.
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia.|Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russia.
authors.forename: N O
authors.initials: NO
authors.lastname: Ronzhin
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia.|Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Baron
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia. bondvs@mail.ru.|Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russia. bondvs@mail.ru.
authors.forename: V S
authors.initials: VS
authors.lastname: Bondar
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia.|Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Gitelson
mesh_terms: D000641:Ammonia / Q000032:analysis; D006868:Hydrolysis; D058612:Nanodiamonds / Q000737:chemistry*; D014508:Urea / Q000032:analysis / Q000737:chemistry*; D014510:Urease / Q000737:chemistry
chemical_list: D058612:Nanodiamonds; D000641:Ammonia; D014508:Urea; D014510:Urease