title: C21 steroidal glycosides from Cynanchum stauntonii induce apoptosis in HepG2 cells.
abstract: Two new (1-2) and three known (3-5) C21 steroidal glycosides were isolated from Cynanchum stauntonii. Their structures were elucidated on the basis of 1D and 2D-NMR spectroscopic data as well as HRTOFMS analysis. The cytotoxicity of the compounds against A549, HepG2, and 4T1 cell lines were evaluated by MTT assay. Compound 4 exhibited good inhibitory activities with the IC50 values 26.82, 12.24, and 44.12 μM, respectively. Furthermore, compound 4 could induce G1 phase arrest, upregulate the expression levels of caspases-3, -9, and Bax, and downregulate the expression level of Bcl-2. These results indicated that compound 4 might be valuable to anticancer drug candidates.
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: cpu-yzq@cpu.edu.cn.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Yin
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Shu-Le
authors.initials: SL
authors.lastname: Yu
authors.affiliation: The First Clinical Medical Institute, Nanjing Medical University, Nanjing 210000, China.
authors.forename: Yu-Jian
authors.initials: YJ
authors.lastname: Wei
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Zheng-Feng
authors.initials: ZF
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products & Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhang
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, and WHO Collaborating Center for Tradition Medicine, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products & Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China.
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, and WHO Collaborating Center for Tradition Medicine, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Chun-Tao
authors.initials: CT
authors.lastname: Che
authors.affiliation: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China. Electronic address: zjwonderful@hotmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D031105:Cynanchum / Q000737:chemistry*; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D051381:Rats; D013256:Steroids / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D013256:Steroids
keywords: Apoptosis; C(21) steroidal glycoside; Caspase; Cynanchum stauntonii