title: Interrogating Circulating Microsomes and Exosomes Using Metal Nanoparticles.
abstract: A chip-based approach for electrochemical characterization and detection of microsomes and exosomes based on direct electro-oxidation of metal nanoparticles (MNPs) that specifically recognize surface markers of these vesicles is reported. It is found that exosomes and microsomes derived from prostate cancer cells can be identified by their surface proteins EpCAM and PSMA, suggesting the potential of exosomes and microsomes for use as diagnostic biomarkers.
authors.affiliation: Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.
authors.forename: Yi-Ge
authors.initials: YG
authors.lastname: Zhou
authors.affiliation: Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.
authors.forename: Reza M
authors.initials: RM
authors.lastname: Mohamadi
authors.affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
authors.forename: Mahla
authors.initials: M
authors.lastname: Poudineh
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3M2, Canada.
authors.forename: Leyla
authors.initials: L
authors.lastname: Kermanshah
authors.affiliation: Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.
authors.forename: Sharif
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
authors.forename: Tina Saberi
authors.initials: TS
authors.lastname: Safaei
authors.affiliation: Division of Urology, Sunnybrook Research Institute, University of Toronto, Toronto, ON, M4M 3M5, Canada.
authors.forename: Jessica
authors.initials: J
authors.lastname: Stojcic
authors.affiliation: Division of Urology, Sunnybrook Research Institute, University of Toronto, Toronto, ON, M4M 3M5, Canada.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Nam
authors.affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
authors.forename: Edward H
authors.initials: EH
authors.lastname: Sargent
authors.affiliation: Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.|Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3M2, Canada.|Department of Biochemistry, University of Toronto, Toronto, ON, M5S 3M2, Canada.
authors.forename: Shana O
authors.initials: SO
authors.lastname: Kelley
mesh_terms: D045744:Cell Line, Tumor; D004563:Electrochemistry; D055354:Exosomes / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008861:Microsomes / Q000378:metabolism* / Q000648:ultrastructure; D011471:Prostatic Neoplasms / Q000097:blood
keywords: electrochemistry; exosomes; microsomes; nanoparticles; prostate cancer