title: Integrin αVβ3 and αVβ5 are required for leukemia inhibitory factor-mediated the adhesion of trophoblast cells to the endometrial cells.
abstract: The embryo implantation including adhesion between trophoblast and endometrium is a crucial process for the successful pregnancy. LIF and adhesion molecules including integrins are known as significant factors for embryo implantation. However, the function of LIF on the regulation of adhesion molecule expression and promotion of trophoblast adhesion to endometrial cells has not been fully elucidated. Here we show that LIF significantly induced mRNA expression of ITGAV, ITGB3, and ITGB5 in endometrial cells, as evidenced by RT-PCR and qRT-PCR analysis. Based on the results from treatment of antagonist for LIF receptor (hLA), LIF positively regulates expression of integrin αV, β3, and β5, and adhesion of the human trophectoderm-derived JAr cells to endometrial Ishikawa cells. Furthermore, the adhesion between trophoblastic cells and LIF-stimulated endometrial cells was significantly reduced by neutralization of LIF-mediated integrin β3 and β5 expression on endometrial cell surface with integrin subunit β3 and β5 antibodies. Taken together, we firstly demonstrate that LIF enhances the adhesion of trophoblastic cells to endometrial cells by up-regulating expression of integrin heterodimer αVβ3 and αVβ5, indicating the promotion of endometrial receptivity for embryo implantation.
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan, Gyeongsangnam-do 626-780, Republic of Korea.
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Chung
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan, Gyeongsangnam-do 626-780, Republic of Korea.
authors.forename: Mi-Ju
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Laboratory of Molecular Toxicology, School of Pharmacy, SungKyunKwan University, Suwon, Gyeonggi-do, Republic of Korea.
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan, Gyeongsangnam-do 626-780, Republic of Korea. Electronic address: hjchoi@pusan.ac.kr.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan, Gyeongsangnam-do 626-780, Republic of Korea. Electronic address: hagis@pusan.ac.kr.
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Ha
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D010064:Embryo Implantation / Q000502:physiology; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D019030:Receptors, Vitronectin / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D039302:Integrin alphaVbeta3; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5
keywords: Endometrium; Implantation; Integrin; Leukemia inhibitory factor; Trophoblast