title: Effect of Eudragit on In Vitro Transfection Efficiency of PEI-DNA Complexes.
abstract: OBJECTIVE
  Eudragit® E 100 (EE100) was used to improve the transfection efficiency of polyethylenimine (PEI).
  
  
  METHODS
  Mobility of PEI-DNA complexes with and without EE100 were visualized by agarose gel electrophoresis and their transfection efficiencies were investigated in KB human oral carcinoma cells by flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to determine the viability of transfected cells.
  
  
  RESULTS
  Gel electrophoresis illustrated formation of complete complexes at N/P ratios above 5. PEI had the highest transfection efficiency at an N/P ratio of 15, whereas in combination with EE100, the transfection efficiency was highest at an N/P ratio of 7.5. High concentrations of EE100 in combination with PEI were found to reduce cell viability.
  
  
  CONCLUSIONS
  The results show a synergistic action of EE100 in transfection of DNA at low N/P ratios compared to PEI alone.
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A.
authors.forename: Naveen
authors.initials: N
authors.lastname: Kanthamneni
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A. The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, U.S.A.
authors.forename: Bryant
authors.initials: B
authors.lastname: Yung
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A. NSF Nanoscales Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, OH, U.S.A. The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, U.S.A. lee.1339@osu.edu.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
mesh_terms: D000179:Acrylates / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry / Q000633:toxicity; D004261:DNA Replication; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection / Q000379:methods*
chemical_list: D000179:Acrylates; C477020:Eudragit E100; D011108:Polymers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D004247:DNA
keywords: Eudragit; Gene therapy; KB cells; PEI–DNA complex; cationic polymer; gene delivery; gene transfer; polyethylenimine