title: Development of a localized surface plasmon resonance-based gold nanobiosensor for the determination of prolactin hormone in human serum.
abstract: A localized surface plasmon resonance immunoassay has been developed to determine prolactin hormone in human serum samples. Gold nanoparticles were synthesized, and the probe was prepared by electrostatic adsorption of antibody on the surfaces of gold nanoparticles. The pH and the antibody-to-gold nanoparticle ratio, as the factors affecting the probe functions, were optimized. The constructed nanobiosensor was characterized by dynamic light scattering. The sensor was applied for the determination of prolactin antigen concentration based on the amount of localized surface plasmon resonance peak shift. A linear dynamic range of 1-40 ng ml(-1), a detection limit of 0.8 ng ml(-1), and sensitivity of 10 pg ml(-1) were obtained. Finally, the nanobiosensor was applied for the determination of prolactin in human control serum sample.
authors.affiliation: Department of Chemistry and Nanochemistry, Faculty of Sciences and Modern Technologies, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Faridli
authors.affiliation: Department of Chemistry and Nanochemistry, Faculty of Sciences and Modern Technologies, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: mahani@kgut.ac.ir.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Mahani
authors.affiliation: Department of Biotechnology, Institute of Science, High Technology, and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Torkzadeh-Mahani
authors.affiliation: Nuclear Science and Technology Research Institute, Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Fasihi
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D000067493:Dynamic Light Scattering; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D011388:Prolactin / Q000097:blood*; D020349:Surface Plasmon Resonance
chemical_list: D000906:Antibodies; D006046:Gold; D011388:Prolactin
keywords: Gold nanoparticles; Immunoassay; Label-free detection; Localized surface plasmon resonance; Nanobiosensor; Prolactin