title: MicroRNA 28-5p regulates ATP-binding cassette transporter A1 via inhibiting extracellular signal-regulated kinase 2.
abstract: The biological function of the intronic microRNA-28 (miR-28) may be associated with the biological roles of its host gene, LIM domain lipoma‑preferred partner (LPP). LPP has been reported to promote smooth muscle cell migration in arterial injury and atherosclerosis. However, the mechanism of miR‑28 in atherosclerosis remains unclear. In the current study, the aim was to validate the inhibitory effect of miR‑28‑5p on extracellular signal‑regulated kinase 2 (ERK2), to investigate its biological role in atherosclerosis and its association with cardiovascular disease. Western blotting and stem‑loop reverse transcription‑quantitative polymerase chain reaction combined with TaqMAN microRNA analysis was conducted. The current study demonstrated that miR‑28‑5p upregulated the expression of ATP‑binding cassette transporter A1 (ABCA1) via the inhibition of ERK2 in HepG2 cells. In addition, increased levels of plasma miR‑28‑5p were positively correlated with the levels of high‑density lipoprotein cholesterol in patients with unstable angina. This suggests that miR-28-5p participates in atherosclerosis via ERK2-mediated upregulation of the ABCA1 pathway.
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory Center, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: Department of Cardiology, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Center, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Center, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Center, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Shang
authors.affiliation: Department of Clinical Laboratory Center, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Yun-Tao
authors.initials: YT
authors.lastname: Zhou
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000378:metabolism*; D000789:Angina, Unstable / Q000097:blood / Q000201:enzymology / Q000235:genetics; D001665:Binding Sites; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D008076:Cholesterol, HDL; D005419:Flavonoids; C570628:MIRN28 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D019950:Mitogen-Activated Protein Kinase 1; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one