title: 'Candidatus Liberibacter asiaticus', Causal Agent of Citrus Huanglongbing, Is Reduced by Treatment with Brassinosteroids.
abstract: Huanglongbing (HLB) constitutes the most destructive disease of citrus worldwide, yet no established efficient management measures exist for it. Brassinosteroids, a family of plant steroidal compounds, are essential for plant growth, development and stress tolerance. As a possible control strategy for HLB, epibrassinolide was applied to as a foliar spray to citrus plants infected with the causal agent of HLB, 'Candidatus Liberibacter asiaticus'. The bacterial titers were reduced after treatment with epibrassinolide under both greenhouse and field conditions but were stronger in the greenhouse. Known defense genes were induced in leaves by epibrassinolide. With the SuperSAGE technology combined with next generation sequencing, induction of genes known to be associated with defense response to bacteria and hormone transduction pathways were identified. The results demonstrate that epibrassinolide may provide a useful tool for the management of HLB.
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Canales
authors.affiliation: Chemistry Faculty, University of Havana, Havana, Cuba.
authors.forename: Yamilet
authors.initials: Y
authors.lastname: Coll
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Hernández
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Roxana
authors.initials: R
authors.lastname: Portieles
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Mayra
authors.initials: M
authors.lastname: Rodríguez García
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Yunior
authors.initials: Y
authors.lastname: López
authors.affiliation: Group of Phytopathology, Instituto de Investigaciones en Fruticultura Tropical, Havana, Cuba.
authors.forename: Miguel
authors.initials: M
authors.lastname: Aranguren
authors.affiliation: Empresa Agroindustrial Victoria de Girón, Jagüey Grande, Matanzas, Cuba.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Alonso
authors.affiliation: Empresa Agroindustrial Victoria de Girón, Jagüey Grande, Matanzas, Cuba.
authors.forename: Roger
authors.initials: R
authors.lastname: Delgado
authors.affiliation: Group of Phytopathology, Instituto de Investigaciones en Fruticultura Tropical, Havana, Cuba.
authors.forename: Maritza
authors.initials: M
authors.lastname: Luis
authors.affiliation: Group of Phytopathology, Instituto de Investigaciones en Fruticultura Tropical, Havana, Cuba.
authors.forename: Lochy
authors.initials: L
authors.lastname: Batista
authors.affiliation: Group of Phytopathology, Instituto de Investigaciones en Fruticultura Tropical, Havana, Cuba.
authors.forename: Camilo
authors.initials: C
authors.lastname: Paredes
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Meilyn
authors.initials: M
authors.lastname: Rodríguez
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Merardo
authors.initials: M
authors.lastname: Pujol
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Ochagavia
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Viviana
authors.initials: V
authors.lastname: Falcón
authors.affiliation: Department of Genomics and Breeding, Iwate Biotechnology Research Center, Kitakami, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Terauchi
authors.affiliation: Gene Research Center, Shinshu University, Ueda, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsumura
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Camilo
authors.initials: C
authors.lastname: Ayra-Pardo
authors.affiliation: Group of Phytopathology, Instituto de Investigaciones en Fruticultura Tropical, Havana, Cuba.
authors.forename: Raixa
authors.initials: R
authors.lastname: Llauger
authors.affiliation: Department of Physiology, National Institute of Agricultural Science, Mayabeque, Cuba.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Pérez
authors.affiliation: Department of Physiology, National Institute of Agricultural Science, Mayabeque, Cuba.
authors.forename: Mirian
authors.initials: M
authors.lastname: Núñez
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan, 48824, United States of America.
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Borrusch
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan, 48824, United States of America.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Walton
authors.affiliation: Group of Phytopathology, Tobacco Research Institute, Havana, Cuba.
authors.forename: Yussuan
authors.initials: Y
authors.lastname: Silva
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Eulogio
authors.initials: E
authors.lastname: Pimentel
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Carlos
authors.initials: C
authors.lastname: Borroto
authors.affiliation: Department of Plant Science, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Orlando
authors.initials: O
authors.lastname: Borrás-Hidalgo
mesh_terms: D060406:Brassinosteroids / Q000494:pharmacology*; D002957:Citrus / Q000187:drug effects / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012230:Rhizobiaceae / Q000187:drug effects*
chemical_list: D060406:Brassinosteroids