title: Enhancing cell kill in vitro from hyperthermia through pre-sensitizing with ultrasound-stimulated microbubbles.
abstract: Ultrasound-stimulated microbubbles (MBs) were demonstrated to enhance cell kill from hyperthermia. Definity MBs were injected into wells containing 4T1 cells in culture media and scanned with 1-MHz ultrasound, an exposure duration of 30 s and a negative pressure of 0.5 or 1.3 MPa. Some cell samples were placed in a water bath heated to 42 °C for 5 min. Cell death was quantified. When combining MBs, ultrasound at 1.3 MPa and hyperthermia, more than 58.8% ± 7.21% of cells were nonviable. When exposed to hyperthermia alone or exposure to MBs and ultrasound but no hyperthermia, cell death was less than 10.1% ± 6.96% and 30.1% ± 10.8%, respectively.
authors.affiliation: Bioacoustic Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 North Mathews, Urbana, Illinois 61801, USA ghoshal2@gmail.com, oelze@illinois.edu.
authors.forename: Goutam
authors.initials: G
authors.lastname: Ghoshal
authors.affiliation: Bioacoustic Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 North Mathews, Urbana, Illinois 61801, USA ghoshal2@gmail.com, oelze@illinois.edu.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Oelze
mesh_terms: D000818:Animals; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D003287:Contrast Media / Q000737:chemistry*; D005260:Female; D005466:Fluorocarbons / Q000737:chemistry*; D006979:Hyperthermia, Induced; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D045423:Microbubbles; D014464:Ultrasonic Therapy / Q000379:methods*; D000069453:Ultrasonic Waves
chemical_list: D003287:Contrast Media; D005466:Fluorocarbons; C042852:perflutren