title: Association of anti-Ro52, anti-Ro60 and anti-La antibodies with diagnostic, clinical and laboratory features in a referral hospital in Jerez, Spain.
abstract: OBJECTIVE
  Several antibodies have proven to be useful in autoimmune diseases, as markers for diagnosis, prognosis or clinical manifestations. Our objective was to evaluate the diagnosis and manifestations associated for antibodies anti-Ro52, anti-Ro60 and anti-La at a referral hospital in Spain.
  
  
  METHODS
  We retrospectively analyzed the antigenic specificities of the consecutive samples submitted to the Immunology Unit for antinuclear antibody screening between 2002 and 2012. We included patients with more than one positive sample for some of the autoantibodies anti-Ro52, anti-Ro60 or anti-La. We also reviewed diagnosis, clinical and laboratory features. As dependent variable we evaluated possible combinations of anti-Ro52, anti-Ro60 and anti-La.
  
  
  RESULTS
  322 patients, 91% females, were studied (age 44.3±15.51 years). The most frequent diagnosis was Sjögren's syndrome (40.06%) and systemic lupus erythematosus (SLE) (36.6%). The most prevalent pattern by indirect immunofluorescence was the fine speckled (69.9%). Anti-Ro52+/anti-Ro60+/anti-La+ combination was positively associated with fine speckled pattern (p: 0.001) and negatively with homogeneous (p: 0.016) and cytoplasmic pattern (p: 0.002). Isolated anti-Ro52+ was negatively associated with fine speckled pattern (p<0.001) and positively with the cytoplasmic one (p<0.001). The main positive associations with clinical symptoms were xerostomia and xerophthalmia with anti-Ro52+/anti-Ro60+/anti-La+ (p<0.001), oral ulcers with anti-Ro52+/anti-Ro60+/anti-La- (p: 0.002) and alopecia with anti-Ro52-/anti-Ro60+/anti-La- (p: 0.003). Negative associations were xerophthalmia and photosensitivity with anti-Ro52+/anti-Ro60-/anti-La- (p: 0.003). Laboratory positive associations were hypergammaglobulinemia with anti-Ro52+/anti-Ro60+/anti-La+ (p: 0.003), and hypocomplementemia with anti-Ro52-/anti-Ro60+/anti-La- (p: 0.003). Leucopenia was negatively associated with anti-Ro52+/anti-Ro60-/anti-La- (p: 0.003).
  
  
  CONCLUSIONS
  Our study found significant relationships between clinical and laboratory manifestations with different patterns of antibodies to anti-Ro52, anti-Ro60 and anti-La. The combination of antibodies might be clinically useful due to prognostic and therapeutic implications.
authors.affiliation: Dept. of Rheumatology, Hospital de Jerez, Jerez de la Frontera, Spain. Electronic address: menoralmagro@hotmail.com.
authors.forename: Raúl
authors.initials: R
authors.lastname: Menor Almagro
authors.affiliation: Dept. of Immunology, Hospital Reina Sofía, Córdoba, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Jurado Roger
authors.affiliation: Dept. of Immunology, Hospital de Jerez, Jerez de la Frontera, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Rodríguez Gutiérrez
authors.affiliation: Hospital Comarcal Virgen del Camino, Sanlucar, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Solís Díaz
authors.affiliation: Centro de Investigación Clínica de Morelia SC, Morelia, Mexico.
authors.forename: Mario Humberto
authors.initials: MH
authors.lastname: Cardiel
authors.affiliation: Dept. of Rheumatology, Hospital de Jerez, Jerez de la Frontera, Spain.
authors.forename: José Javier
authors.initials: JJ
authors.lastname: Salaberri Maestrojuan
mesh_terms: D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000097:blood*; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020733:RNA, Small Cytoplasmic / Q000276:immunology*; D012189:Retrospective Studies; D012216:Rheumatic Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D012261:Ribonucleoproteins / Q000276:immunology*; D013030:Spain; D062606:Tertiary Care Centers; D000097585:SS-B Antigen; D000099207:SS-A Antigen
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; D015415:Biomarkers; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human
keywords: Anti-La/SS-B antibodies; Anti-Ro/SS-A antibodies; Anticuerpo anti-La/SSB; Anticuerpo anti-Ro/SSA; Autoimmune disease; Enfermedades autoinmunes; Immunofluorescence patterns; Patrones de inmunofluorescencia indirecta