title: Ketamine effect on HMGB1 and TLR4 expression in rats with acute lung injury.
abstract: Acute lung injury (ALI) is a common emergency and severe case in clinic. High mobility group protein box 1 (HMGB1) can be treated as a new anti-inflammatory treatment target. Toll-like receptor 4 (TLR4) is an important receptor of HMGB1. Ketamine is a widely used intravenous anesthetic with good anti-inflammatory and immune regulating function. Whether it can protect ALI through inhibiting HMGB1 and TLR4 expression in lung tissue still needs further investigation. Male SD rats were randomly divided into control, lipopolysaccharide (LPS) group and ketamine intervention group with 15 rats in each group. The rats were euthanatized at 24 h after modeling and the bronchoalveolar lavage fluid (BALF) was collected for HMGB1 and TLR4 level detection. Western Blot was applied to analyze HMGB1 and TLR4 protein expression in the lung tissue. HMGB1 and TLR4 concentration in BALF were 5.369 ± 1.564 ng/ml and 43.980 ± 7.524 pg/ml in the control, respectively. They were 12.358 ± 4.681 ng/ml and 102.538 ± 8.412 pg/ml in LPS group, and 7.399 ± 2.346 ng/ml and 87.208 ± 7.558 pg/ml in ketamine intervention group, respectively. Their levels increased significantly in LPS group and down-regulated after ketamine intervention. HMGB1 and TLR4 protein expression in lung tissue elevated obviously in LPS group, and decreased after ketamine treatment. HMGB1 and TLR4 protein level showed positive correlation in lung tissue (r = 0.921, P < 0.001). Ketamine can inhibit HMGB1 and TLR4 expression in ALI, and alleviate LPS induced rat lung injury.
authors.affiliation: Department of Anesthesiology, Wuhan General Hospital of Guangzhou Command Wuhan 430070, China.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Qin
authors.affiliation: Department of Anesthesiology, The 154 Hospital, People's Liberation Army Xinyang 464000, Henan Province, China.
authors.forename: Qiu-Han
authors.initials: QH
authors.lastname: Gu
authors.affiliation: Department of Anesthesiology, Wuhan General Hospital of Guangzhou Command Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.affiliation: Department of Anesthesiology, Wuhan General Hospital of Guangzhou Command Wuhan 430070, China.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, Wuhan General Hospital of Guangzhou Command Wuhan 430070, China.
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Gan
authors.affiliation: Department of Anesthesiology, Wuhan General Hospital of Guangzhou Command Wuhan 430070, China.
authors.forename: Zhong-Bin
authors.initials: ZB
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology, Wuhan General Hospital of Guangzhou Command Wuhan 430070, China.
authors.forename: Bi-Xi
authors.initials: BX
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D024243:HMGB1 Protein / Q000096:biosynthesis / Q000187:drug effects*; D007649:Ketamine / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D007649:Ketamine
keywords: HMGB1; Ketamine; TLR4; acute lung injury