title: [Methylation Status of PTPL1 Gene in Non-Hodgkin's Lymphoma Cells].
abstract: OBJECTIVE
  To investigate the methylation status of PTPL1 in Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines, and the reversing effect of 5-Aza on expression of PTPL1 mRNA.
  
  
  METHODS
  The Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines were cultured in vitro, the MS-PCR was used to detect the methylation status of PTPL1 gene and RT-PCR was used to detect the expression of PTPL1 mRNA, the CCK-8 method was used to determine the cell proliferation, the siRNA-mediated silencing of PTPL1 was used to clarify the role of PTPL1 in lymphoma cell lines.
  
  
  RESULTS
  PTPL1 gene promoter was non-methylated in Hut78, Maver and Z138 cells, and was hyper-methylated in Raji, CA46 and Jurkat cells; the different levels of PTPL1 mRNA expression were in Hut78, Maver and Z138; the re-expression of PTPL1 mRNA resulted from hyper-methylation was found in Raji, CA46 and Jurkat cells. The 5-Aza treatment could inhibit the prolifenation of Raji, CA46 and Jurkat cell lines and induce re-expression of PTPL1 mRNA. After PTPL1 was interfered by siRNA, the growth of lymphoma cells was promoted.
  
  
  CONCLUSIONS
  The PTPL1 gene silencing induced by hyper-methylation may be an important factor in the occurrence and development of non-Hodgkin lymphoma. The methylation of PTPL1 gene may be used as a possible molecular marker for diagnosis and treatment targets.
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China. E-mail: jinghm@yahoo.com.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Jing
mesh_terms: D049109:Cell Proliferation; D019175:DNA Methylation; D020868:Gene Silencing; D006801:Humans; D019169:Jurkat Cells; D008228:Lymphoma, Non-Hodgkin; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13
chemical_list: C516742:PTPN13 protein, human; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13