title: [Clinical significance of cysteinyl leukotriene receptor expression in primary hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate expression of the cysteinyl leukotriene receptor (CysLT1R) in hepatocellular carcinoma (HCC) tissues and determine its clinical significance.
  
  
  METHODS
  Cancerous and paraneoplastic liver tissues were collected from 30 patients with HCC and from 12 patients with liver hemangioma patients (controls). Real-time PCR and immunohistochemistry were used to evaluate the expression of CysLT1R in these tissues and assess the relationship with clinical pathological features. T-test,?Fisher's exact test and Kaplan-Meier survival analysis were used for statistical analysis.
  
  
  RESULTS
  The expression of CysLT1R in adjacent liver tissues (100%) was higher than that in the HCC (43.33%, P = 0.000) and normal liver tissues (41.67%, P = 0.000). The level of CysLT1R mRNA was also higher in paraneoplastic liver tissues (0.0339+/-0.0221) than in the paired HCC tissues (0.0127+/-0.0116, t = 2.911, P = 0.008) and normal liver tissues (0.0154+/-0.0123, t = -2.310, P = 0.033). There was no difference between the levels in HCC and normal liver tissues (P more than 0.05). Higher level of CysLT1R mRNA, higher level of serum alpha-fetoprotein, and higher tumor stage (III-IV) were associated with poor prognosis (respectively 4.372, P = 0.037; 24.187, P = 0.000; 8.75, P = 0.003). However, no evident relationship between the expression of CysLT1R and other clinical features was observed. Conclusions Overexpression of CysLT1R may contribute to the occurrence and progression of HCC.
authors.affiliation: Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Xizhen
authors.initials: X
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Wanhua
authors.initials: W
authors.lastname: Ren
authors.forename: Jianting
authors.initials: J
authors.lastname: Fang
authors.forename: Yaru
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Ji
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism*; D016022:Case-Control Studies; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018077:Receptors, Leukotriene / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018077:Receptors, Leukotriene; D000509:alpha-Fetoproteins; C083169:leukotriene D4 receptor