title: 1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain.
abstract: DCL-1 (CD302) is a single-pass type one transmembrane protein which is predominantly expressed on myeloid cell lines. It possess the ability of endocytosis and is assumed to play a role in cell adhesion and migration. It has been also connected to several illnesses but more on the level of mRNA than on the protein expression level. More interestingly it is alternatively expressed in the form of a fusion protein with another single-pass type one transmembrane protein DEC205 (CD205) which is normally involved in antigen-uptake and endocytosis. The fusion protein has been assigned to have altered function compared to the wild type proteins. We have performed NMR structural analysis of the 16.2 kDa extracellular domain of DCL-1 to get a better insight onto this molecule. We have been able to assign nearly 97 % of resonance frequencies for the (15)N and (13)C labeled recombinant protein. The assignments have been deposited into Biological Magnetic Resonance Data Bank under the accession number 25802.
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic.|Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Pospíšilová
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic.|Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kavan
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic.|Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic. chmelik@biomed.cas.cz.|Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. chmelik@biomed.cas.cz.
authors.forename: Josef
authors.identifier: http://orcid.org/0000-0002-0305-9345
authors.initials: J
authors.lastname: Chmelík
mesh_terms: D005110:Extracellular Space; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D011956:Receptors, Cell Surface / Q000737:chemistry*
chemical_list: C477741:CD302 protein, human; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface
keywords: C-type lectin like receptor; DCL-1 (CD302); Heteronuclear NMR spectroscopy; Protein NMR; Resonance assignment