title: The study of mismatch repair in endometrial cancer patients with a family history of cancer.
abstract: OBJECTIVE
  To assess the expression of mismatch repair (MMR) proteins MSH2 and MLH1 and carry out microsatellite analysis in patients with endometrial cancer (EC) with regard to the family history of cancer.
  
  
  METHODS
  Morphological and immunohistochemical study was performed on tumor tissue samples of 49 EC patients. Microsatellite instability was determined using PCR with primers which flank microsatellite region BAT-26.
  
  
  RESULTS
  A tendency to a decreased expression of both MSH2 and MLH1 markers in a group of EC patients with a family history of cancer as compared with a group without aggregation of cancer in family history was observed (labeling index - LI - was 36.1 ± 8.1% and LI 20.7 ± 9.1% versus LI 48.0 ± 5.8% and 33.8 ± 5.8%, respectively). It was determined that the number of EC patients with tumors deficient by expression of MMR markers was reliably higher in a group of patients with a family history of cancer than in a group of patients without aggregation of cancer in family history (р < 0.05). It was shown that in a group of EC patients with a family history of cancer, MMR-proficient tumors were detected in 38.5% of cases. Microsatellite instability was determined in 10.7% of EC patients including one patient with aggregation of Lynch-associated tumors in family history.
  
  
  CONCLUSIONS
  Family history of cancer of EC patients is associated with malfunctioning of the MMR system as well as may be related to alternative molecular mechanisms.
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: L G
authors.initials: LG
authors.lastname: Buchynska
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: O V
authors.initials: OV
authors.lastname: Brieieva
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: K A
authors.initials: KA
authors.lastname: Nekrasov
authors.affiliation: National Cancer Institute of MH of Ukraine, Kyiv 03022, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Nespryadko
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D053843:DNA Mismatch Repair / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor