title: In Vivo Deep Tissue Fluorescence and Magnetic Imaging Employing Hybrid Nanostructures.
abstract: Breakthroughs in nanotechnology have made it possible to integrate different nanoparticles in one single hybrid nanostructure (HNS), constituting multifunctional nanosized sensors, carriers, and probes with great potential in the life sciences. In addition, such nanostructures could also offer therapeutic capabilities to achieve a wider variety of multifunctionalities. In this work, the encapsulation of both magnetic and infrared emitting nanoparticles into a polymeric matrix leads to a magnetic-fluorescent HNS with multimodal magnetic-fluorescent imaging abilities. The magnetic-fluorescent HNS are capable of simultaneous magnetic resonance imaging and deep tissue infrared fluorescence imaging, overcoming the tissue penetration limits of classical visible-light based optical imaging as reported here in living mice. Additionally, their applicability for magnetic heating in potential hyperthermia treatments is assessed.
authors.affiliation: Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Madrid 28049, Spain.|Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS , Ctra. Colmenar km. 9.100, Madrid 28034, Spain.
authors.forename: Dirk H
authors.initials: DH
authors.lastname: Ortgies
authors.affiliation: iMdea-Nanociencia, Campus Universitario de Cantoblanco , Madrid 28049, Spain.
authors.forename: Leonor
authors.initials: L
authors.lastname: de la Cueva
authors.affiliation: Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Madrid 28049, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Del Rosal
authors.affiliation: Fluorescence Imaging Group, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Darwin 2, Madrid 28049, Spain.|Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS , Ctra. Colmenar km. 9.100, Madrid 28034, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Sanz-Rodríguez
authors.affiliation: Fluorescence Imaging Group, Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid , Avda. Arzobispo Morcillo 2, Madrid 28029, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Fernández
authors.affiliation: Fluorescence Imaging Group, Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid , Avda. Arzobispo Morcillo 2, Madrid 28029, Spain.|Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS , Ctra. Colmenar km. 9.100, Madrid 28034, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Iglesias-de la Cruz
authors.affiliation: iMdea-Nanociencia, Campus Universitario de Cantoblanco , Madrid 28049, Spain.|Nanobiotecnología (IMDEA-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC) , Madrid, Spain.
authors.forename: Gorka
authors.initials: G
authors.lastname: Salas
authors.affiliation: iMdea-Nanociencia, Campus Universitario de Cantoblanco , Madrid 28049, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Cabrera
authors.affiliation: iMdea-Nanociencia, Campus Universitario de Cantoblanco , Madrid 28049, Spain.|Nanobiotecnología (IMDEA-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC) , Madrid, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Teran
authors.affiliation: Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Madrid 28049, Spain.|Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS , Ctra. Colmenar km. 9.100, Madrid 28034, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Jaque
authors.affiliation: Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Madrid 28049, Spain.|Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS , Ctra. Colmenar km. 9.100, Madrid 28034, Spain.
authors.forename: Emma
authors.initials: E
authors.lastname: Martín Rodríguez
mesh_terms: D000818:Animals; D005453:Fluorescence; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use; D036103:Nanotechnology
chemical_list: D058185:Magnetite Nanoparticles
keywords: bioimaging; fluorescence imaging; iron oxide nanoparticles; magnetic resonance imaging; multimodal imaging; nanohybrids