title: In vivo and in vitro studies of borate based glass micro-fibers for dermal repairing.
abstract: Full-thickness skin defects represent urgent clinical problem nowadays. Wound dressing materials are hotly needed to induce dermal reconstruction or to treat serious skin defects. In this study, the borate bioactive glass (BG) micro-fibers were fabricated and compared with the traditional material 45S5 Bioglass(®) (SiG) micro-fibers. The morphology, biodegradation and bioactivity of BG and SiG micro-fibers were investigated in vitro. The wound size reduction and angiogenic effects of BG and SiG micro-fibers were evaluated by the rat full-thickness skin defect model and Microfil technique in vivo. Results indicated that the BG micro-fibers showed thinner fiber diameter (1 μm) and better bioactivity than the SiG micro-fibers did. The ionic extracts of BG and SiG micro-fibers were not toxic to human umbilical vein endothelial cells (HUVECs). In vivo, the BG micro-fiber wound dressings obviously enhanced the formation of blood vessel, and resulted in a much faster wound size reduction than the SiG micro-fibers, or than the control groups, after 9 days application. The good skin defect reconstruction ability of BG micro-fibers contributed to the B element in the composition, which results in the better bioactivity and angiogenesis. As shown above, the novel bioactive borate glass micro-fibers are expected to provide a promising therapeutic alternative for dermal reconstruction or skin defect repair.
authors.affiliation: School of Materials Science and Engineering, Tong Ji University, Shanghai 200092, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Materials Science and Engineering, Tong Ji University, Shanghai 200092, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China.
authors.forename: Shichang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: School of Materials Science and Engineering, Tong Ji University, Shanghai 200092, People's Republic of China. Electronic address: zhounai@tongji.edu.cn.
authors.forename: Nai
authors.initials: N
authors.lastname: Zhou
authors.affiliation: School of Materials Science and Engineering, Tong Ji University, Shanghai 200092, People's Republic of China.
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, Tong Ji University, Shanghai 200092, People's Republic of China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Tong Ji University, Shanghai 200092, People's Republic of China.
authors.forename: Deping
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China. Electronic address: shzhangchangqing@163.com.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry*; D001881:Borates / Q000737:chemistry* / Q000494:pharmacology; D002516:Ceramics / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D051381:Rats; D012867:Skin / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D001881:Borates; C425330:bioactive glass 45S5
keywords: Bioactivity; Borate bioactive glass micro-fibers; Wound healing