title: Analysis and Quantitation of Glycated Hemoglobin by Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry.
abstract: Measurement of glycated hemoglobin is widely used for the diagnosis and monitoring of diabetes mellitus. Matrix assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometry (MS) analysis of patient samples is used to demonstrate a method for quantitation of total glycation on the β-subunit of hemoglobin. The approach is accurate and calibrated with commercially available reference materials. Measurements were linear (R(2) > 0.99) across the clinically relevant range of 4% to 20% glycation with coefficients of variation of ≤ 2.5%. Additional and independent measurements of glycation of the α-subunit of hemoglobin are used to validate β-subunit glycation measurements and distinguish hemoglobin variants. Results obtained by MALDI-TOF MS were compared with those obtained in a clinical laboratory using validated HPLC methodology. MALDI-TOF MS sample preparation was minimal and analysis times were rapid making the method an attractive alternative to methodologies currently in practice.
authors.affiliation: SimulTOF Systems, Sudbury, MA, 01776, USA. stephen.hattan@simultof.com.
authors.forename: Stephen J
authors.identifier: http://orcid.org/0000-0003-2836-7093
authors.initials: SJ
authors.lastname: Hattan
authors.affiliation: SimulTOF Systems, Sudbury, MA, 01776, USA.
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Parker
authors.affiliation: SimulTOF Systems, Sudbury, MA, 01776, USA.
authors.forename: Marvin L
authors.initials: ML
authors.lastname: Vestal
authors.affiliation: Department of Pathology, University of California San Diego, La Jolla, CA, 92093-0612, USA.
authors.forename: Jane Y
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Department of Pathology, University of California San Diego, La Jolla, CA, 92093-0612, USA.|VA San Diego Healthcare System, PALMS, MS 113, San Diego, CA, 92161, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Herold
authors.affiliation: Division of Endocrinology, Metabolism, and Diabetes, University of Colorado School of Medicine, MS 8106, Aurora, CO, 80045, USA.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Duncan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000175:diagnosis; D006442:Glycated Hemoglobin / Q000032:analysis*; D055554:Hemoglobin Subunits / Q000032:analysis*; D006801:Humans; D016014:Linear Models; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D006442:Glycated Hemoglobin; D055554:Hemoglobin Subunits
keywords: Diabetes; Glycation; Hemoglobin; MALDI-TOF; Matrix assisted laser desorption ionization; Quantitation