title: Pore alterations of the endothelial lining of rat fenestrated intestinal capillaries exposed to acute stress.
abstract: Stress-induced inflammatory responses in the portal system are characterized by elevations in serum concentrations of interleukin-6 (IL-6) and endotoxins such as lipopolysaccharides (LPS). LPS translocation from the intestinal to the capillary lumen occurs via LPS endocytosis by the capillary endothelium. Because the capillary endothelium of the small intestinal submucosa is fenestrated, we determined the role of pore modifications within the fenestrated endothelium in relaying inflammatory stress responses in the portal vein. We evaluated changes in the diameter and density of endothelial pores of the lamina propria of intestinal villi induced by continuous light (CL) exposure for 48 h and the correlation between these changes and serum IL-6 concentration in the portal vein in a rat model. We found significant increases in both the pore diameter and density, accompanied by a significant increase in portal IL-6 concentration; these changes were significantly attenuated by pretreatment with propranolol, a beta adrenergic receptor antagonist. In contrast, intravenous noradrenaline administration mimicked CL-induced modifications of the diameter and density of pores and the elevation of portal vein IL-6 concentration. These findings suggested that stress-induced inflammatory responses in the portal system may be a part of the modifications of the endothelial pores triggered by sympathetic activation.
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Aosa
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan. schiba@oita-u.ac.jp.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Chiba
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kitamura
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ina
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Tatsukawa
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Chinatsu
authors.initials: C
authors.lastname: Moriwaki
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Huixing
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Koro
authors.initials: K
authors.lastname: Gotoh
authors.affiliation: Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Masaki
authors.affiliation: Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kakuma
authors.affiliation: Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Shibata
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, Oita University, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Fujikura
mesh_terms: D000818:Animals; D002196:Capillaries / Q000473:pathology* / Q000648:ultrastructure*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000473:pathology* / Q000648:ultrastructure*; D004797:Enzyme-Linked Immunosorbent Assay; D007413:Intestinal Mucosa / Q000098:blood supply; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000473:pathology*