title: Generation of liver-specific TGF-α/c-Myc-overexpressing porcine induced pluripotent stem-like cells and blastocyst formation using nuclear transfer.
abstract: Transgenic porcine induced pluripotent stem (iPS) cells are attractive cell sources for the development of genetically engineered pig models, because they can be expanded without senescence and have the potential for multiple gene manipulation. They are also useful cell sources for disease modeling and treatment. However, the generation of transgenic porcine iPS cells is rare, and their embryonic development after nuclear transfer (NT) has not yet been reported. We report here the generation of liver-specific oncogenes (TGF-α/c-Myc)-overexpressing porcine iPS (T/M iPS)-like cells. They expressed stem cell characteristics and were differentiated into hepatocyte-like cells that express oncogenes. We also confirmed that NT embryos derived from T/M iPS-like cells successfully developed blastocysts in vitro. As an initial approach toward porcine transgenic iPS cell generation and their developmental competence after NT, this study provides foundations for the efficient generation of genetically modified porcine iPS cells and animal models.
authors.affiliation: Stem Cell Institute-KNU, Kangwon National University, Chuncheon, 200-701, Korea.
authors.forename: Kyung-Mee
authors.initials: KM
authors.lastname: Park
authors.forename: Joohyeong
authors.initials: J
authors.lastname: Lee
authors.forename: Kamal Hany
authors.initials: KH
authors.lastname: Hussein
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Se-Ran
authors.initials: SR
authors.lastname: Yang
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
authors.forename: Heung-Myong
authors.initials: HM
authors.lastname: Woo
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D013552:Swine; D046509:Tissue Culture Techniques; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis*
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D016211:Transforming Growth Factor alpha