title: Hyperbaric oxygen preconditioning ameliorates hypoxia-ischemia brain damage by activating Nrf2 expression in vivo and in vitro.
abstract: The present study aimed to investigate whether hyperbaric oxygen preconditioning (HBO-PC) could ameliorate hypoxia-ischemia brain damage (HIBD) by an increase of Nrf2 expression. P7 Sprague-Dawley rats (aged 7 d, n = 195) were used in two in vivo experiments, including BO-PC exposure experiments in non-HIBD models and treatment experiments in HIBD models. 2,3,5-triphenyltetrazolium chloride (TTC) staining, Nissl Staining, and TUNEL staining were performed. And expressions of Nrf2, HO-1, and GSTs were measured. For in vitro studies, oxygen-glucose deprivation cells were established. Morphological and apoptotic staining and gene silencing of Nrf2 by siRNA transfection were investigated. For exposure experiments, HBO-PC for longer time increased the expression of Nrf2 significantly. And for treatment experiments, HBO-PC treatment significantly decreased infarction area, lessened neuronal injury, reduced apoptosis, and increased both the expression of Nrf2 and activities of its downstream proteins. Cytology tests confirmed effects of HBO-PC treatments. Besides, Nrf2 siRNA significantly reduced protective effects of HBO-PC. These observations demonstrated that an up-regulation of Nrf2 by HBO-PC might play an important role in the generation of tolerance against HIBD.
authors.affiliation: a Graduate Management Unit of Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;|b Department of Orthopedics , Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhai
authors.affiliation: c Department of Gastroenterology , Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Han
authors.initials: H
authors.lastname: Lin
authors.affiliation: c Department of Gastroenterology , Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: b Department of Orthopedics , Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: a Graduate Management Unit of Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Jiazi
authors.initials: J
authors.lastname: Shi
authors.affiliation: a Graduate Management Unit of Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Ouyang
authors.initials: O
authors.lastname: Chen
authors.affiliation: d Department of Neurology , Changhai Hospital Affiliated to the Second Military Medical University , Shanghai , PR China ;
authors.forename: Jiasi
authors.initials: J
authors.lastname: Li
authors.affiliation: e Faculty of Naval Medicine , Second Military Medical University , Shanghai , PR China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D005947:Glucose / Q000172:deficiency / Q000494:pharmacology; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D002534:Hypoxia, Brain / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D020925:Hypoxia-Ischemia, Brain / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010100:Oxygen / Q000494:pharmacology*; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D034741:RNA, Small Interfering; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D005982:Glutathione Transferase; D005947:Glucose; D010100:Oxygen
keywords: Hyperbaric oxygen; hypoxia–ischemia brain damage; ischemic tolerance; prevention; reactive oxygen species