title: Upregulation of microRNA-31 targeting integrin α5 suppresses tumor cell invasion and metastasis by indirectly regulating PI3K/AKT pathway in human gastric cancer SGC7901 cells.
abstract: To verify the hypothesis that upregulation of microRNA-31 (miR-31) targeting integrin α5 (ITGA5) suppresses tumor cell invasion and metastasis by indirectly regulating phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway in human SGC7901 gastric cancer (GC) cells. The miRTarBase was used to predict whether ITGA5 is the target gene of miR-31, which was further confirmed by luciferase reporter gene assay. The SGC7901 GC cells were divided into five groups including the blank, miR-31 mimic, miR-31 mimic control, miR-31 inhibitor, and miR-31 inhibitor control groups. Reverse transcriptase-polymerase chain reaction (RT-PCR), western blotting, cell scratch test, and transwell assays were respectively performed in our study. TGA5 was found as the target gene of miR-31. The RT-PCR detection revealed that, compared with the blank group, ITGA5 messenger RNA (mRNA) expression decreased in the miR-31 mimic group, but increased in the miR-31 inhibitor group. The western blotting examination suggested that the expressions of ITGA5, PI3K, and AKT proteins reduced in the miR-31 mimic group, but enhanced in the miR-31 inhibitor group when compared to the blank group, respectively. The cell scratch and transwell assays indicated that the miR-31 expressions were negatively associated with GC cell migration and invasion. Besides, RT-PCR combined with western blotting demonstrated that the miR-31 expressions were higher in the normal tissues than those in the GC tissues, while the ITGA5 mRNA and protein showed lower expression in the normal tissues than they did in the GC tissues. Our study concluded that upregulation of miR-31 targeting ITGA5 may suppress tumor cell invasion and metastasis by indirectly regulating PI3K/AKT signaling pathway in human SGC7901 GC cells.
authors.affiliation: Department of Gastrointestinal Colorectal Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130000, People's Republic of China.
authors.forename: Xue-Bin
authors.initials: XB
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, The First Hospital of Jilin University, Changchun, 130000, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: Graduate School, Changchun University of Chinese Medicine, Changchun, 130000, People's Republic of China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Wen
authors.affiliation: Department of Cadre Ward, The First Hospital of Jilin University, Changchun, 130000, People's Republic of China.
authors.forename: Xiao-Xue
authors.initials: XX
authors.lastname: Bai
authors.affiliation: Department of Gastrointestinal Colorectal Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130000, People's Republic of China.
authors.forename: Zhen-Juan
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Endoscopics, China-Japan Union Hospital of Jilin University, Xiantai Da Road, No 126, Changchun, 130000, People's Republic of China. lj.ma@139.com.
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Ma
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D039482:Integrin alpha5 / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: D039482:Integrin alpha5; C534612:MIRN31 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: Cell invasion; Cell migration; Gastric cancer; Integrin α5; MicroRNA-31; Phosphoinositide 3-kinase (PI3K); Protein kinase B (AKT)