title: Transcriptome mining: Multigene panel to test delousing drug response in the sea louse Caligus rogercresseyi.
abstract: Controlling infestations of copepodid ectoparasites in the salmon industry is increasingly problematic given higher instances of drug resistance or loss of sensitivity. Despite the importance of this issue, the molecular mechanisms and genes implicated in resistance/susceptibility are only scarcely understood. The objective of the present study was to identify and evaluate the expression levels of candidate genes associated with delousing drug response in the sea louse Caligus rogercresseyi. From RNA-seq data obtained for adult male and female sea lice, 62.48 M reads were assembled in 70,349 high-quality contigs. BLASTX analysis against UniprotKB/Swiss-Prot and the ESTs available for crustaceans in the NCBI database identified 870 transcripts previously related to genes associated with delousing drug response. Furthermore, 14 candidate genes were validated through RT-qPCR and were evaluated with deltamethrin and azamethiphos bioassays. The results evidenced an overregulation of genes involved in ion transport in salmon lice treated with deltamethrin, while those treated with azamethiphos evidenced an overregulation of genes such as cytochrome P450, Carboxylesterase, and acetylcholine receptors. The present study provides a multigene panel to test delousing drug response to pyrethroids and organophosphates in a highly prevalent pathogen of the Chilean salmon industry.
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), Department of Oceanography, University of Concepción, P.O. Box 160-C, Chile.
authors.forename: V
authors.initials: V
authors.lastname: Valenzuela-Muñoz
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), Department of Oceanography, University of Concepción, P.O. Box 160-C, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: C
authors.initials: C
authors.lastname: Gallardo-Escárate
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology; D033342:Copepoda / Q000187:drug effects* / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009570:Nitriles / Q000494:pharmacology*; D063086:Organothiophosphates / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D000977:Antiparasitic Agents; D009570:Nitriles; D063086:Organothiophosphates; D011722:Pyrethrins; C017180:decamethrin; D012313:RNA; C072763:azamethiphos
keywords: Caligus rogercresseyi; Organophosphates; Pyrethroids; RNA-seq; RT-qPCR