title: Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation.
abstract: Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous cytoplasmic NSP3 expression (wild-type or deletion mutant) and electroporated rotavirus-like and standard synthetic mRNAs. This assay shows that the last four GACC nucleotides of viral mRNA are essential for efficient translation and that both the NSP3 eIF4G- and RNA-binding domains are required. We also show efficient translation of rotavirus-like mRNAs even with a 5'UTR as short as 5 nucleotides, while more than eleven nucleotides are required for the 3'UTR. Despite the weak requirement for a long 5'UTR, a good AUG environment remains a requirement for rotavirus mRNAs translation.
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Gratia
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Vende
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Charpilienne
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
authors.forename: Hilma Carolina
authors.initials: HC
authors.lastname: Baron
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Laroche
authors.affiliation: INSERM UMR-1037 - Université de Toulouse III-Paul Sabatier, Laboratoire d'Excellence Toulouse Cancer (TOUCAN), Equipe labellisée Ligue Contre le Cancer Toulouse, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Sarot
authors.affiliation: INSERM UMR-1037 - Université de Toulouse III-Paul Sabatier, Laboratoire d'Excellence Toulouse Cancer (TOUCAN), Equipe labellisée Ligue Contre le Cancer Toulouse, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Pyronnet
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.|Université d'Evry Val d'Essonne, Département de Biologie, Evry, France.
authors.forename: Mariela
authors.initials: M
authors.lastname: Duarte
authors.affiliation: Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Poncet
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; C082740:NSP3 protein, Rotavirus; D012333:RNA, Messenger; D017361:Viral Nonstructural Proteins