title: Structural characterization and in vitro biomedical activities of sulfated chitosan from Sepia pharaonis.
abstract: A low molecular weight sulfated chitosan (SP-LMWSC) was isolated from the cuttlebone of Sepia pharaonis. Elemental analysis established the presence of C, H and N. The sulfation of SP-LMWSC was confirmed by the presence of characteristic peaks in FT-IR and FT-Raman spectra. The thermal properties of SP-LMWSC were studied by thermogravimetric analysis and differential scanning calorimetry. Electrolytic conductivity of SP-LMWSC was measured by cyclic voltammetry and the molecular weight was determined by MALDI-TOF/MS. The molecular structure and sulfation sites of SP-LMWSC were unambiguously confirmed using (1)H, (13)C, 2D COSY and 2D HSQC NMR spectroscopy. SP-LMWSC exhibited increased anticoagulant activity in avian blood by delaying coagulation parameters and displayed cytostatic activity by inhibiting the migration of avian leucocytes. SP-LMWSC demonstrated avian antiviral activity by binding to Newcastle disease virus receptors at a low titer value of 1/64. These findings suggested that SP-LMWSC isolated from an industrial discard holds immense potentials as carbohydrate based pharmaceuticals in future.
authors.affiliation: Department of Medical Biotechnology, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, Tamil Nadu, India.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Karthik
authors.affiliation: Department of Medical Biotechnology, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, Tamil Nadu, India.
authors.forename: Venkatesan
authors.initials: V
authors.lastname: Manigandan
authors.affiliation: Department of Marine Pharmacology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, Tamil Nadu, India. Electronic address: saran_prp@yahoo.com.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Saravanan
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
authors.forename: Rajaian Pushpabai
authors.initials: RP
authors.lastname: Rajesh
authors.affiliation: Sophisticated Analytical Instrument Facility, Indian Institute of Technology, Chennai, India.
authors.forename: Baby
authors.initials: B
authors.lastname: Chandrika
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight; D049833:Sepia / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013329:Structure-Activity Relationship; D013818:Thermogravimetry
chemical_list: D000925:Anticoagulants; D000998:Antiviral Agents; C480355:chitosan sulfate; D048271:Chitosan
keywords: Avian biomedical application; Structural characterization; Sulfated chitosan