title: [Isolation and Identification of Human Umbilical Cord and Placenta-derived Stem Cells and Their Component Analysis].
abstract: OBJECTIVE
  Clinical transplantation evidence has been indicated that umbilical cord blood (UCB) can be useful in the hematopoietic reconstitution in the children, but can not be well in the adult hematopoietic transplantation because of the low count. This study was to evaluate a new method for collecting stem cells from human placenta and umbilical cord, and to comparatively analyze the similarity and difference of quality and quantity of the cells.
  
  
  METHODS
  The UCB was collected, in same time the placental tissue was sterily collected; the umbilical placenta was collected by perfusing the blood (UPB) cord arterial and venous vascules with 0.9% saline; the mesenchymal stem cells (MSC) from same source of umbilical cord and placental tissues were isolated and cultured. The cell colony assay and flow cytometry were performed to determine the proliferation capacities and cell markers of UMSC and PMSC.
  
  
  RESULTS
  The total nuclear cells (NC) and hematopoietic stem cells (CD34(+)) from UPB and UCB were (17.45 ± 16.86) × 10(8), (6.9 ± 4.61) × 10(8) and (2.97 ± 2.25) × 10(6), (1.91 ± 1.7) × 10(6), respectively. Furthermore, the UPB contained more early precursor of hematopoietic stem cells (CD33(+) CD34(-)) (6.2 ± 13.5) × 10(5), (0.2 ± 0.8) × 10(5) ; and high proportion of MSC to NC (25.21 ± 18.69, 0.05 ± 0.10)%, respectively in 62 samples. There were no difference of the MSC level in UPB and UCB, as well as in the morphology and cell markers.
  
  
  CONCLUSIONS
  UPB has rich hematopoietic stem cells and mesenchymal stem cells. Placenta may offer another source for hematopoietic stem cells in research of hematopoietic stem cells and regeneration medicine.
authors.affiliation: Cell Park (Jiangsu) Stem Cell Bio-Engineering Company, LTD, Yancheng dongtai 224200, Jiangsu Province, China. E-mail: victor@cell-park.com.
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Zhou
authors.affiliation: Cell Park (Jiangsu) Stem Cell Bio-Engineering Company, LTD, Yancheng dongtai 224200, Jiangsu Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Cell Park (Jiangsu) Stem Cell Bio-Engineering Company, LTD, Yancheng dongtai 224200, Jiangsu Province, China.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Su
authors.affiliation: Cell Park (Jiangsu) Stem Cell Bio-Engineering Company, LTD, Yancheng dongtai 224200, Jiangsu Province, China.
authors.forename: Yuan-Hong
authors.initials: YH
authors.lastname: Qi
authors.affiliation: Cell Park (Jiangsu) Stem Cell Bio-Engineering Company, LTD, Yancheng dongtai 224200, Jiangsu Province, China.
authors.forename: Zheng-Kai
authors.initials: ZK
authors.lastname: Si
mesh_terms: D000328:Adult; D018952:Antigens, CD34; D002469:Cell Separation; D005260:Female; D005312:Fetal Blood; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D010920:Placenta; D011247:Pregnancy; D014470:Umbilical Cord
chemical_list: D018952:Antigens, CD34