title: The Influence of Ghrelin on the Development of Dextran Sodium Sulfate-Induced Colitis in Rats.
abstract: Ghrelin has protective and therapeutic effects in the gut. The aim of present studies was to investigate the effect of treatment with ghrelin on the development of colitis evoked by dextran sodium sulfate (DSS). Methods. Studies have been performed on rats. Colitis was induced by adding 5% DSS to the drinking water for 5 days. During this period animals were treated intraperitoneally twice a day with saline or ghrelin given at the dose of 8 nmol/kg/dose. On the sixth day, animals were anesthetized and the severity of colitis was assessed. Results. Treatment with ghrelin during administration of DSS reduced the development of colitis. Morphological features of colonic mucosa exhibited a reduction in the area and deep of mucosal damage. Ghrelin reversed the colitis-induced decrease in blood flow, DNA synthesis, and superoxide dismutase activity in colonic mucosa. These effects were accompanied by a decrease in the colitis-evoked increase in mucosal concentration of interleukin-1β and malondialdehyde. Treatment with ghrelin reversed the DSS-induced reduction in body weight gain. Conclusions. Administration of ghrelin exhibits the preventive effect against the development of DSS-induced colitis. This effect seems to be related to ghrelin's anti-inflammatory and antioxidative properties.
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland ; Department of Anatomy, Faculty of Medicine, Jagiellonian University Medical College, 12 Kopernika Street, 31-034 Cracow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Matuszyk
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland ; Department of Pediatrics, Gastroenterology and Nutrition, Children's Hospital, Faculty of Medicine, Jagiellonian University Medical College, 256 Wielicka Street, 30-663 Cracow, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Ceranowicz
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Warzecha
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ceranowicz
authors.affiliation: Department of Pediatrics, Gastroenterology and Nutrition, Children's Hospital, Faculty of Medicine, Jagiellonian University Medical College, 256 Wielicka Street, 30-663 Cracow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Fyderek
authors.affiliation: Department of Pathology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Gałązka
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Cieszkowski
authors.affiliation: Department of Medical Physiology, Faculty of Health Sciences, Jagiellonian University Medical College, 12 Michałowskiego Street, 31-126 Cracow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bonior
authors.affiliation: Department of Medical Physiology, Faculty of Health Sciences, Jagiellonian University Medical College, 12 Michałowskiego Street, 31-126 Cracow, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jaworek
authors.affiliation: Department of Dental Prosthetics, Institute of Dentistry, Faculty of Medicine, Jagiellonian University Medical College, 4 Montelupich Street, 31-155 Cracow, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pihut
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Dembiński
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D054439:Ghrelin / Q000008:administration & dosage*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D054439:Ghrelin; D053583:Interleukin-1beta; D008315:Malondialdehyde; D016264:Dextran Sulfate