title: A Novel Strategy for Softening Gelatin-Bioactive-Glass Hybrids.
abstract: The brittle structure of polymer-bioactive-glass hybrids is a hurdle for their biomedical applications. To address this issue here, we developed a novel method to cease the overcondensation of bioactive-glass by polymer cross-linking. Here, an organosilane-functionalized gelatin methacrylate (GelMA) is covalently bonded to a bioactive-glass during the sol-gel process, and the condensation of silica networks is controlled by photo-cross-linking of GelMA. The physicochemical properties and mechanical strength of these hybrids are tunable by the incorporation of secondary cross-linking agents. These hydrogels display elastic properties with ultimate compression strain above 0.2 mm·mm(-1) and tunable compressive modulus in the range of 42-530 kPa. In addition, these hydrogels are bioactive because they promoted the alkaline phosphatase activity of bone progenitor cells. They are also well-tolerated in the mice subcutaneous model. Therefore, our method is efficient for the prevention of overcondensation and allows preparation of soft bioactive hydrogels from organic-inorganic matrices, suitable for soft and hard tissue regeneration.
authors.affiliation: School of Chemical & Biomolecular Engineering, University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Ali
authors.initials: A
authors.lastname: Negahi Shirazi
authors.affiliation: School of Chemical & Biomolecular Engineering, University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Ali
authors.initials: A
authors.lastname: Fathi
authors.forename: Francia Garces
authors.initials: FG
authors.lastname: Suarez
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Peter K
authors.initials: PK
authors.lastname: Maitz
authors.affiliation: School of Chemical & Biomolecular Engineering, University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Fariba
authors.initials: F
authors.lastname: Dehghani
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D005780:Gelatin / Q000494:pharmacology*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D020007:Implants, Experimental; D008422:Materials Testing / Q000379:methods*; D008689:Methacrylates / Q000494:pharmacology; D051379:Mice; D017646:Organosilicon Compounds / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010758:Phosphorus / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry; D017640:Silicates / Q000737:chemistry
chemical_list: D008689:Methacrylates; D017646:Organosilicon Compounds; D017640:Silicates; C437167:poly(ethylene glycol)diacrylate; D010758:Phosphorus; D011092:Polyethylene Glycols; D005780:Gelatin; D002118:Calcium
keywords: bioactive-glasses; organic−inorganic hybrids; photo-cross-linking; sol−gel; tissue engineering