title: Synergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype.
abstract: Hypoxia is a pathalogical condition in which tissues are deprived of adequate oxygen supply. The hypoxia effect on tumors has a critically important role on maintenance of cancer stem cell phenotype. The aim of this study is to investigate the effects of hypoxia on cancer stem cells on three dimensional (3D) in vitro culture models. Osteosarcoma stem cells characterized by CD133 surface protein were isolated from osteosarcoma cell line (SaOS-2) by magnetic-activated cell sorting (MACS) technique. Isolated CD133(+) and CD133(-) cells were cultivated under hypoxic (1% O2) and normoxic conditions (21% O2) for 3 days. For the 3D model, bacterial cellulose scaffold was used as the culture substrate. 3D morphologies of cells were examined by scanning electron microscopy (SEM); RT-PCR and immunocytochemistry staining were used to demonstrate conservation of the cancer stem cell phenotype in 3D environment under hypoxic conditions. Cell viability was shown by MTT assay on 3. and 7. culture days. This study is seen as an introduction to develop a 3D hypoxic cancer stem cell based tumor model to study CSC behavior and tumor genesis in vitro.
authors.affiliation: Stem Cell Department, Institute of Health Sciences, Ege University, Izmir, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Gorgun
authors.affiliation: Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey.
authors.forename: Sukru
authors.initials: S
authors.lastname: Ozturk
authors.affiliation: Histology and Embryology Department, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Sevtap
authors.initials: S
authors.lastname: Gokalp
authors.affiliation: Histology and Embryology Department, Faculty of Medicine, Celal Bayar University, Manisa, Turkey; Experimental Health Research Center of Health Sciences, Near East University, Nicosia, North Cyprus.
authors.forename: Seda
authors.initials: S
authors.lastname: Vatansever
authors.affiliation: Stem Cell Department, Institute of Health Sciences, Ege University, Izmir, Turkey; Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey; Bioengineering Department, Faculty of Engineering, Ege University, Izmir, Turkey.
authors.forename: S Ismet Deliloglu
authors.initials: SI
authors.lastname: Gurhan
authors.affiliation: Stem Cell Department, Institute of Health Sciences, Ege University, Izmir, Turkey; Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey; Bioengineering Department, Faculty of Engineering, Ege University, Izmir, Turkey. Electronic address: aylin.sendemir@ege.edu.tr.
authors.forename: Aylin Sendemir
authors.initials: AS
authors.lastname: Urkmez
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism; D002452:Cell Count; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D048430:Cell Shape; D006801:Humans; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation; D014411:Neoplastic Stem Cells / Q000473:pathology*; D012516:Osteosarcoma / Q000473:pathology*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D055153:Stem Cell Niche
chemical_list: D000071916:AC133 Antigen; D012333:RNA, Messenger
keywords: 3D Tumor Model; Cancer Stem Cells; Hypoxia