title: Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide.
abstract: Osteoprogenitor cells play a significant role in the growth or repair of bones, and have great potential as cell sources for regenerative medicine and bone tissue engineering, but control of their specific differentiation into bone cells remains a challenge. Graphene-based nanomaterials are attractive candidates for biomedical applications as substrates for stem cell (SC) differentiation, scaffolds in tissue engineering, and components of implant devices owing to their biocompatible, transferable and implantable properties. This study examined the enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs) by reduced graphene oxide (rGO) nanoparticles (NPs), and rGO NPs was prepared by reducing graphene oxide (GO) with a hydrazine treatment followed by annealing in argon and hydrogen. The cytotoxicity profile of each particle was examined using a water-soluble tetrazolium-8 (WST-8) assay. At different time-points, a WST-8 assay, alkaline phosphatase (ALP) activity assay and alizarin red S (ARS) staining were used to determine the effects of rGO NPs on proliferation, differentiation and mineralization, respectively. The results suggest that graphene-based materials have potential as a platform for stem cells culture and biomedical applications.
authors.forename: Linhua
authors.initials: L
authors.lastname: Jin
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Oh Seong
authors.initials: OS
authors.lastname: Jin
authors.forename: Yong Cheol
authors.initials: YC
authors.lastname: Shin
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Suck Won
authors.initials: SW
authors.lastname: Hong
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Han
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D006108:Graphite / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology
chemical_list: D010087:Oxides; D006108:Graphite