title: Synthesis of Luminescent Graphene Quantum Dots with High Quantum Yield and Their Toxicity Study.
abstract: High fluorescence quantum yield graphene quantum dots (GQDs) have showed up as a new generation for bioimaging. In this work, luminescent GQDs were prepared by an ameliorative photo-Fenton reaction and a subsequent hydrothermal process using graphene oxide sheets as the precursor. The as-prepared GQDs were nanomaterials with size ranging from 2.3 to 6.4 nm and emitted intense green luminescence in water. The fluorescence quantum yield was as high as 24.6% (excited at 340 nm) and the fluorescence was strongest at pH 7. Moreover, the influences of low-concentration (12.5, 25 μg/mL) GQDs on the morphology, viability, membrane integrity, internal cellular reactive oxygen species level and mortality of HeLa cells were relatively weak, and the in vitro imaging demonstrated GQDs were mainly in the cytoplasm region. More strikingly, zebrafish embryos were co-cultured with GQDs for in vivo imaging, and the results of heart rate test showed the intake of small amounts of GQDs brought little harm to the cardiovascular of zebrafish. GQDs with high quantum yield and strong photoluminescence show good biocompatibility, thus they show good promising for cell imaging, biolabeling and other biomedical applications.
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.|State Key Laboratory for Oxo Synthesis and Selective, Lanzhou Institute of Chemical Physics, Lanzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Jiang
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.|State Key Laboratory for Oxo Synthesis and Selective, Lanzhou Institute of Chemical Physics, Lanzhou, China.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.|Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Oxo Synthesis and Selective, Lanzhou Institute of Chemical Physics, Lanzhou, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006108:Graphite / Q000138:chemical synthesis*; D006367:HeLa Cells; D006321:Heart / Q000033:anatomy & histology; D006801:Humans; D049408:Luminescent Agents / Q000138:chemical synthesis*; D008163:Luminescent Measurements / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology
chemical_list: D049408:Luminescent Agents; D017382:Reactive Oxygen Species; D006108:Graphite