title: Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2+ in Hansenula polymorpha.
abstract: Processes taking place in the secretory organelles require Ca2+ and Mn2+, which in yeast are supplied by the Pmr1 ion pump. Here we observed that in the yeast Hansenula polymorpha Ca2+ deficiency in the secretory pathway caused by Pmr1 inactivation is exacerbated by (i) the ret1-27 mutation affecting COPI-mediated vesicular transport, (ii) inactivation of the vacuolar Ca2+ ATPase Pmc1 and (iii) inactivation of Vps35, which is a component of the retromer complex responsible for protein transport between the vacuole and secretory organelles. The ret1-27 mutation also exerted phenotypes indicating alterations in transport between the vacuole and secretory organelles. These data indicate that ret1-27, pmc1 and vps35 affect a previously unknown Pmr1-independent route of the Ca2+ delivery to the secretory pathway. We also observed that the vacuolar protein carboxypeptidase Y receives additional modifications of its glycoside chains if it escapes the Vps10-dependent sorting to the vacuole.
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Fokina
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Maria B
authors.initials: MB
authors.lastname: Chechenova
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Azamat V
authors.initials: AV
authors.lastname: Karginov
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Ter-Avanesyan
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Michael O
authors.initials: MO
authors.lastname: Agaphonov
mesh_terms: D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D020755:Coat Protein Complex I / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D056726:Genetic Association Studies; D006056:Golgi Apparatus / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010843:Pichia / Q000235:genetics* / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D020755:Coat Protein Complex I; D005656:Fungal Proteins; D008345:Manganese; D000252:Calcium-Transporting ATPases; D002118:Calcium