title: Downregulation of fatty acid synthase complex suppresses cell migration by targeting phosphor-AKT in bladder cancer.
abstract: The aim of the present study was to investigate the effect of fatty acid synthase complex (FASN) on the migration capacity of bladder transitional cell carcinoma (BTCC) cells and the involvement of matrix metalloproteinase‑9 (MMP‑9) via targeting of phospho‑AKT (p‑AKT). FASN‑specific small‑interfering RNA (FASN‑siRNA) was used to inhibit FASN gene expression in the 5637 and 253J BTCC cell lines. The knockdown efficiency of FAM‑conjugated FASN‑siRNA was confirmed by fluorescence microscopy. The migratory abilities of BTCC cells were assessed using a Transwell assay. Furthermore, protein and mRNA expression of FASN, p‑AKT, AKT, and migration‑associated protein MMP‑9 were detected by western blot analysis. Treatment with FASN inhibitor Cer and FASN‑siRNA decreased the migratory capacity of bladder cancer cells and reduced the levels of p‑AKT as well as the expression of MMP‑9. These results indicated that FASN inhibition suppressed the migratory capacity of BTCC cells through suppressing AKT activation and consequently reducing MMP‑9 expression. Targeting FASN may represent a promising novel therapeutic strategy for BTCC.
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Zheng
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Jian-Gang
authors.initials: JG
authors.lastname: Gao
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Liu
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Bo-Wen
authors.initials: BW
authors.lastname: Weng
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Si-Chuan
authors.initials: SC
authors.lastname: Hou
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002569:Cerulenin / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology*
chemical_list: D034741:RNA, Small Interfering; D002569:Cerulenin; D064429:Fatty Acid Synthases; D051057:Proto-Oncogene Proteins c-akt