title: Efficacy of polysaccharide from Alcaligenes xylosoxidans MSA3 administration as protection against γ-radiation in female rats.
abstract: Damage to normal tissues is a consequence of both therapeutic and accidental exposures to ionizing radiation. A water-soluble heteropolysaccharide called AXEPS, composed of glucose, galactose, rhamnose and glucouronic acid in a molar ratio of nearly 1.0:1.6:0.4:2.3, respectively, was isolated from culture medium of strain Alcaligenes xylosoxidans MSA3 by ethanol precipitation followed by freeze-drying. Chemical analysis, Fourier-transform infrared (FTIR) and chromatographic studies revealed that the molecular weight was 1.6 × 10(4) g mol(-1). This study was designed to investigate the radioprotective and biological effects of AXEPS in alleviating the toxicity of ionizing radiation in female albino rats. A total of 32 female albino rats were divided into four groups. In the control group, rats were administered vehicle by tube for four weeks. The second group was administered AXEPS (100 mg/kg) orally by gavage for four weeks. Animals in the third group were exposed to whole-body γ-rays (5 Gy) and remained for 2 weeks without treatment. The fourth group received AXEPS (100 mg/kg) orally by gavage for two weeks before being exposed to whole-body γ-rays (5 Gy), then 24 h post γ-rays, they received AXEPS (100 mg/kg) in a treatment continuing till the end of the experiment (15 days after the whole-body γ-irradiation). Oral administration of AXEPS (100 mg/kg) significantly reversed the oxidative stress effects of radiation, as evidenced by the decrease in DNA damage in the bone marrow. Assessment of apoptosis and cell proliferation markers revealed that caspase-3 significantly increased in the irradiated group. Moreover, a significant decrease in the hematological constituents of peripheral blood, the chemotactic index and CD8+ T cells were observed in animals in the irradiation-only group, whereas an increase in the lymphocyte index was observed in animals in that group. In contrast, AXEPS treatment prevented these alterations. From our results, we conclude that AXEPS is a potent antioxidant and treatment agent for protection from γ-rays.
authors.affiliation: Department of Radioisotopes, Nuclear Research Centre, Atomic Energy Authority, Egypt aml_h@hotmail.com.
authors.forename: Amal I
authors.initials: AI
authors.lastname: Hassan
authors.affiliation: Department of Radioisotopes, Nuclear Research Centre, Atomic Energy Authority, Egypt.
authors.forename: Mona A M
authors.initials: MA
authors.lastname: Ghoneim
authors.affiliation: Microbial Biotechnology Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, 12311, Egypt.
authors.forename: Manal G
authors.initials: MG
authors.lastname: Mahmoud
authors.affiliation: Microbial Biotechnology Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, 12311, Egypt.
authors.forename: Mohsen M S
authors.initials: MM
authors.lastname: Asker
authors.affiliation: Microbial Biotechnology Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, 12311, Egypt.
authors.forename: Saher S
authors.initials: SS
authors.lastname: Mohamed
mesh_terms: D000421:Alcaligenes / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays; D016678:Genome; D008970:Molecular Weight; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D023382:Toxicity Tests, Acute; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011135:Polysaccharides, Bacterial; D011837:Radiation-Protective Agents; D004247:DNA
keywords: Alcaligenes xylosoxidans; chemical structure; oxidative stress; polysaccharide; γ rays