title: Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors.
abstract: The cerebellar external granule layer (EGL) is the site of the largest transit amplification in the developing brain, and an excellent model for studying neuronal proliferation and differentiation. In addition, evolutionary modifications of its proliferative capability have been responsible for the dramatic expansion of cerebellar size in the amniotes, making the cerebellum an excellent model for evo-devo studies of the vertebrate brain. The constituent cells of the EGL, cerebellar granule progenitors, also represent a significant cell of origin for medulloblastoma, the most prevalent paediatric neuronal tumour. Following transit amplification, granule precursors migrate radially into the internal granular layer of the cerebellum where they represent the largest neuronal population in the mature mammalian brain. In chick, the peak of EGL proliferation occurs towards the end of the second week of gestation. In order to target genetic modification to this layer at the peak of proliferation, we have developed a method for genetic manipulation through ex vivo electroporation of cerebellum slices from embryonic Day 14 chick embryos. This method recapitulates several important aspects of in vivo granule neuron development and will be useful in generating a thorough understanding of cerebellar granule cell proliferation and differentiation, and thus of cerebellum development, evolution and disease.
authors.affiliation: MRC Centre of Developmental Neurobiology, King's College London.
authors.forename: Michalina
authors.initials: M
authors.lastname: Hanzel
authors.affiliation: MRC Centre of Developmental Neurobiology, King's College London.
authors.forename: Richard J T
authors.initials: RJ
authors.lastname: Wingate
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary, University of London; thomas.butts@qmul.ac.uk.
authors.forename: Thomas
authors.initials: T
authors.lastname: Butts
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002531:Cerebellum / Q000166:cytology* / Q000196:embryology; D002642:Chick Embryo; D003594:Cytoplasmic Granules; D018274:Electroporation / Q000379:methods*; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology; D046509:Tissue Culture Techniques / Q000379:methods*