title: The first crystal structures of RNA-PNA duplexes and a PNA-PNA duplex containing mismatches--toward anti-sense therapy against TREDs.
abstract: PNA is a promising molecule for antisense therapy of trinucleotide repeat disorders. We present the first crystal structures of RNA-PNA duplexes. They contain CUG repeats, relevant to myotonic dystrophy type I, and CAG repeats associated with poly-glutamine diseases. We also report the first PNA-PNA duplex containing mismatches. A comparison of the PNA homoduplex and the PNA-RNA heteroduplexes reveals PNA's intrinsic structural properties, shedding light on its reported sequence selectivity or intolerance of mismatches when it interacts with nucleic acids. PNA has a much lower helical twist than RNA and the resulting duplex has an intermediate conformation. PNA retains its overall conformation while locally there is much disorder, especially peptide bond flipping. In addition to the Watson-Crick pairing, the structures contain interesting interactions between the RNA's phosphate groups and the Π electrons of the peptide bonds in PNA.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kiliszek
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Banaszak
authors.affiliation: Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Dauter
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland wojtekr@ibch.poznan.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rypniewski
mesh_terms: D020029:Base Pairing; D018360:Crystallography, X-Ray; D006801:Humans; D009223:Myotonic Dystrophy / Q000235:genetics / Q000628:therapy; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000627:therapeutic use; D010455:Peptides / Q000235:genetics; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D016372:RNA, Antisense / Q000737:chemistry / Q000235:genetics* / Q000627:therapeutic use; D019680:Trinucleotide Repeat Expansion / Q000235:genetics*; D018911:Trinucleotide Repeats / Q000235:genetics
chemical_list: D020135:Peptide Nucleic Acids; D010455:Peptides; D016372:RNA, Antisense; C097188:polyglutamine; D012313:RNA