title: Lipoplexes of dicationic gemini surfactants with DNA: Structural features of DNA compaction and transfection efficiency.
abstract: The internal structure of DNA lipoplexes with hydroxyethylated alkylammonium gemini surfactants (GS) with high transfection activity was studied by circular dichroism. It was shown that the efficiency of transfection of HEK293T cells with the pEGFP-N1 circular plasmid was different from zero only in the region of existence of chiral supramolecular DNA-GS complexes and reaches a maximum at concentrations at which the spontaneous aggregation of components is observed.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences, Kazan, Russia. dfaizullin@mail.ru.
authors.forename: D A
authors.initials: DA
authors.lastname: Faizullin
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences, Kazan, Russia.
authors.forename: Yu F
authors.initials: YF
authors.lastname: Zuev
authors.affiliation: Arbuzov Institute of Organic and Physical Chemistry, Kazan Research Center, Russian Academy of Sciences, ul. Akademika Arbuzova 8, Kazan, Tatarstan, 420088, Russia.
authors.forename: L Ya
authors.initials: LY
authors.lastname: Zakharova
authors.affiliation: Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Pokrovsky
authors.affiliation: Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Korobeinikov
authors.affiliation: Kazan (Volga Region) Federal University, ul. Kremlevskaya 18, Kazan, Tatarstan, 420008, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Mukhametzyanov
authors.affiliation: Arbuzov Institute of Organic and Physical Chemistry, Kazan Research Center, Russian Academy of Sciences, ul. Akademika Arbuzova 8, Kazan, Tatarstan, 420088, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Konovalov
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D009690:Nucleic Acid Conformation; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000737:chemistry*; D014162:Transfection / Q000379:methods
chemical_list: D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C560819:butanediyl 1,4-bis(hydroxyethylmethylhexadecylammonium); D004247:DNA