title: Treatment with a Nucleoside Polymerase Inhibitor Reduces Shedding of Murine Norovirus in Stool to Undetectable Levels without Emergence of Drug-Resistant Variants.
abstract: Prolonged norovirus shedding may occur in certain patients, such as organ transplant recipients. We established a mouse model for persistent norovirus infection (using the mouse norovirus MNV.CR6 strain). The nucleoside viral polymerase inhibitor 2'-C-methylcytidine (2CMC), but not favipiravir (T-705), reduced viral shedding to undetectable levels. Viral rebound was observed after stopping treatment, which was again effectively controlled by treatment with 2CMC. No drug-resistant variants emerged.
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
authors.forename: Joana
authors.initials: J
authors.lastname: Rocha-Pereira
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
authors.forename: Jana
authors.initials: J
authors.lastname: Van Dycke
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium johan.neyts@rega.kuleuven.be.
authors.forename: Johan
authors.initials: J
authors.lastname: Neyts
mesh_terms: D000577:Amides / Q000627:therapeutic use; D000818:Animals; D017250:Caliciviridae Infections / Q000188:drug therapy* / Q000821:virology; D003562:Cytidine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000188:drug therapy* / Q000821:virology; D051379:Mice; D018345:Mice, Knockout; D029322:Norovirus / Q000187:drug effects* / Q000254:growth & development; D011719:Pyrazines / Q000627:therapeutic use; D017471:Receptors, Interferon / Q000235:genetics; D066027:Transplant Recipients; D017201:Virus Shedding / Q000187:drug effects*
chemical_list: D000577:Amides; D011719:Pyrazines; D017471:Receptors, Interferon; C513402:2'-C-methylcytidine; D003562:Cytidine; C462182:favipiravir