title: Proteome Differences between Hepatitis B Virus Genotype-B- and Genotype-C-Induced Hepatocellular Carcinoma Revealed by iTRAQ-Based Quantitative Proteomics.
abstract: Hepatitis B virus (HBV) is the main cause of hepatocellular carcinoma (HCC) in southeast Asia where HBV genotype B and genotype C are the most prevalent. Viral genotypes have been reported to significantly affect the clinical outcomes of HCC. However, the underlying molecular differences among different genotypes of HBV virus infected HCC have not been revealed. Here, we applied isobaric tags for relative and absolute quantitation (iTRAQ) technology integrated with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify the proteome differences between the HBV genotypes B- and C-induced HCC. In brief, a total of 83 proteins in the surrounding noncancerous tissues and 136 proteins in the cancerous tissues between HBV genotype-B- and genotype-C-induced HCC were identified, respectively. This information revealed that there might be different molecular mechanisms of the tumorigenesis and development of HBV genotypes B- and C-induced HCC. Furthermore, our results indicate that the two proteins ARFIP2 and ANXA1 might be potential biomarkers for distinguishing the HBV genotypes B- and C-induced HCC. Thus, the quantitative proteomic analysis revealed molecular differences between the HBV genotypes B- and C-induced HCC, and might provide fundamental information for further deep study.
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Wei
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.|Liver Disease Center, The First Affiliated Hospital of Fujian Medical University , Fuzhou 350007, People's Republic of China.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xing
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Liu
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Lin
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences , Beijing 100081, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University , Fuzhou 350025, People's Republic of China.|The Liver Center of Fujian Province, Fujian Medical University , Fuzhou 350025, People's Republic of China.|Liver Disease Center, The First Affiliated Hospital of Fujian Medical University , Fuzhou 350007, People's Republic of China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D002853:Chromatography, Liquid; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome
keywords: ANXA1; ARFIP2; HBV genotype B and genotype C; hepatocellular carcinoma; quantitative proteomics