title: Differences in Akt signaling and metabolism gene expression in the right heart, intraventricular septum and left heart of rodents.
abstract: The right heart is functionally and structurally different from the left heart; however, potential differences in Akt signaling and the expression of metabolic genes between the right heart and left heart in different rodents are still unknown. Using Western blotting and real time quantification polymerase chain reaction, we measured the levels of total Akt, phosphorylated Akt and its downstream targets as well as metabolism genes including glucose transporter 1, glucose transporter 4 (GLUT4), peroxisome proliferator-activated receptor α, peroxisome proliferator-activated receptor γ, peroxisome proliferator-activated receptor δ, peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α), and pyruvate dehydrogenase lipoamide kinase isozyme 4. We found that phosphorylated Akt and proline-rich Akt substrate 40 levels were significantly increased in the RV compared with the LV in rats but only had an increased trend in mice. Correspondingly, GLUT4 was significantly increased in the RV compared with the LV both in mice and rats. PGC-1α was significantly increased in the RV compared with the LV in mice but only had an increased trend in rats. Moreover, Akt signaling activity and metabolism genes' expression in the IVS were similar to the RV in mice but to the LV in rats. There were some differences in the activity of Akt signaling and in the levels of metabolism genes among the right ventricle, interventricular septum and left ventricle. Also, the diversity of activity of Akt and metabolism genes between the right ventricle and left ventricle are different between rats and mice. In conclusion, the activity of Akt signaling and the levels of metabolism genes are different among the right ventricle, interventricular septum and left ventricle providing some potential clues for exploring the roles of Akt signaling and cardiac metabolisms in different parts of the heart. Additionally, the differences in Akt activity and metabolism genes' levels between the right and left ventricles are different between mice and rats, to which we should pay attention when using different animal model in heart study.
authors.affiliation: Department of Cardiology, Gansu Provincial Hospital Lanzhou 730000, Gansu, People's Republic of China ; Department of Cardiology, The First Affiliated Hospital with Nanjing Medical University Nanjing 210029, Jiangsu, People's Republic of China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Cardiology, The First Affiliated Hospital with Nanjing Medical University Nanjing 210029, Jiangsu, People's Republic of China.
authors.forename: Shutong
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Pathology, Gansu Provincial Hospital Lanzhou 730000, Gansu, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital with Nanjing Medical University Nanjing 210029, Jiangsu, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University Tianjin 300384, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital with Nanjing Medical University Nanjing 210029, Jiangsu, People's Republic of China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, Gansu Provincial Hospital Lanzhou 730000, Gansu, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Gansu Provincial Hospital Lanzhou 730000, Gansu, People's Republic of China.
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006346:Heart Septum / Q000378:metabolism*; D006352:Heart Ventricles / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051057:Proto-Oncogene Proteins c-akt / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D013045:Species Specificity; D059467:Transcriptome
chemical_list: D051057:Proto-Oncogene Proteins c-akt
keywords: Akt signaling; Right ventricle; cardiac metabolism; left ventricle; rodent