title: Faecalibacterium prausnitzii A2-165 has a high capacity to induce IL-10 in human and murine dendritic cells and modulates T cell responses.
abstract: Faecalibacterium prausnitzii strain A2-165 was previously reported to have anti-inflammatory properties and prevent colitis in a TNBS model. We compared the immunomodulatory properties of strain A2-165 to four different F. prausnitzii isolates and eight abundant intestinal commensals using human dendritic cells (DCs) and mouse BMDCs in vitro. Principal component analysis revealed that the cytokine response to F. prausnitzii A2-165 is distinct from the other strains in eliciting high amounts of IL-10 secretion. The mouse DNBS model of relapsing IBD was used to compare the protective effects of F. prausnitzii A2-165 and Clostridium hathewayi, a low secretor of IL-10, on the Th1-driven inflammatory response to DNBS; attenuation of disease parameters was only observed with F. prausnitzii. In an in vivo mouse model of nasal tolerance to ovalbumin, F. prausnitzii A2-165 enhanced ovalbumin-specific T cell proliferation and reduced the proportion of IFN-γ(+) T cells in CLNs. Similarly, in vitro F. prausnitzii A2-165 stimulated BMDCs increased ovalbumin-specific T cell proliferation and reduced the number of IFN-γ(+) T cells. These mechanisms may contribute to the anti-inflammatory effects of F. prausnitzii in colitis and support the notion that this abundant bacterium might contribute to immune homeostasis in the intestine via its anti-inflammatory properties.
authors.affiliation: Host-Microbe Interactomics Group, University of Wageningen, 6708 WD, The Netherlands.
authors.forename: Oriana
authors.initials: O
authors.lastname: Rossi
authors.affiliation: Department of Pediatrics, Erasmus Medical Center - Sophia Children's Hospital, 3015 CE, Rotterdam, The Netherlands.
authors.forename: Lisette A
authors.initials: LA
authors.lastname: van Berkel
authors.affiliation: Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, INRA, 78352, Jouy-en-Josas, France.|UMR 1319 Micalis, AgroparisTech, 78352, Jouy-en-Josas, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Chain
authors.affiliation: Department of Medical Microbiology, University Medical Center Groningen, 9700 RB, Groningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Tanweer Khan
authors.affiliation: Host-Microbe Interactomics Group, University of Wageningen, 6708 WD, The Netherlands.
authors.forename: Nico
authors.initials: N
authors.lastname: Taverne
authors.affiliation: Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, INRA, 78352, Jouy-en-Josas, France.|UMR 1319 Micalis, AgroparisTech, 78352, Jouy-en-Josas, France.|Department of Gastroenterology and Nutrition, Hôpital SaintAntoine and UPMC University of Paris, 75012, Paris, France.|Equipe AVENIR Gut Microbiota and Immunity, INSERM U1057/UMR CNRS 7203, Université Pierre et Marie Curie 6, 75005, Paris, France.
authors.forename: Harry
authors.initials: H
authors.lastname: Sokol
authors.affiliation: Microbial Ecology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, AB21 9SB, Aberdeen, United Kingdom.
authors.forename: Sylvia H
authors.initials: SH
authors.lastname: Duncan
authors.affiliation: Microbial Ecology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, AB21 9SB, Aberdeen, United Kingdom.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Flint
authors.affiliation: Department of Medical Microbiology, University Medical Center Groningen, 9700 RB, Groningen, The Netherlands.
authors.forename: Hermie J M
authors.initials: HJ
authors.lastname: Harmsen
authors.affiliation: Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, INRA, 78352, Jouy-en-Josas, France.|UMR 1319 Micalis, AgroparisTech, 78352, Jouy-en-Josas, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: Department of Pediatrics, Erasmus Medical Center - Sophia Children's Hospital, 3015 CE, Rotterdam, The Netherlands.
authors.forename: Janneke N
authors.initials: JN
authors.lastname: Samsom
authors.affiliation: Host-Microbe Interactomics Group, University of Wageningen, 6708 WD, The Netherlands.
authors.forename: Jerry M
authors.initials: JM
authors.lastname: Wells
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003092:Colitis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D003106:Colon / Q000382:microbiology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D000070037:Faecalibacterium prausnitzii / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: C508609:IL10 protein, human; D016328:NF-kappa B; D051193:Toll-Like Receptors; D016753:Interleukin-10