title: Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus.
abstract: Polysaccharides from marine clams perform various biological activities, whereas information on structure is scarce. Here, a water-soluble polysaccharide MMPX-B2 was isolated from Meretrix meretrix Linnaeus. The proposed structure was deduced through characterization and its immunological activity was investigated. MMPX-B2 consisted of d-glucose and d-galctose residues at a molar ratio of 3.51:1.00. The average molecular weight of MMPX-B2 was 510 kDa. This polysaccharide possessed a main chain of (1→4)-linked-α-d-glucopyranosyl residues, partially substituted at the C-6 position by a few terminal β-d-galactose residues or branched chains consisting of (1→3)-linked β-d-galactose residues. Preliminary immunological tests in vitro showed that MMPX-B2 could stimulate the murine macrophages to release various cytokines, and the structure-activity relationship was then established. The present study demonstrated the potential immunological activity of MMPX-B2, and provided references for studying the active ingredients in M. meretrix.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. li792051095@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. eternal83@163.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. jackieqian@163.com.
authors.forename: Jianying
authors.initials: J
authors.lastname: Qian
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. 15061888901@163.com.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. 18262280354@163.com.
authors.forename: Jialin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. zhenming_lu@163.com.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Lu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. zhenghxu@jiangnan.edu.cn.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. shijs@163.com.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Shi
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D049872:Bivalvia; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D012623:Seawater; D013329:Structure-Activity Relationship
chemical_list: D000276:Adjuvants, Immunologic; C000611217:MMPX-B2 polysaccharide, Meretrix meretrix; D011134:Polysaccharides
keywords: Meretrix meretrix Linnaeus; immunological activity; polysaccharide; structure