title: Portable capillary electrophoresis instrument with contactless conductivity detection for on-site analysis of small volumes of biological fluids.
abstract: A novel, easy to use and portable capillary electrophoretic instrument for injection of small volumes of biological fluids equipped with contactless conductivity detection was constructed. The instrument is lightweight (<5 kg), all necessary parts including a tablet computer are accommodated in a plastic briefcase with dimensions 20 cm × 33 cm × 17 cm (w × l × h), allows hydrodynamic injection of small sample volumes and can continuously operate for at least 10 hours. The semi-automated hydrodynamic sample injection is accomplished via a specially designed PMMA interface that is able to repeatedly inject sample aliquots from a sample volume as low as 10 μL, with repeatability of peak areas below 5%. The developed interface and the instrument were optimized for the injection of biological fluids. Practical utility was demonstrated on the determination of formate in blood serum samples from acute methanol intoxication patients and on the analysis of ionic profile (nitrosative stress markers, including nitrite and nitrate) in the exhaled breath condensate from one single exhalation.
authors.affiliation: Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, Czech Republic; Department of Chemistry, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Greguš
authors.affiliation: Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Foret
authors.affiliation: Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, Czech Republic. Electronic address: petr.kuban@ceitec.muni.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Kubáň
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D001944:Breath Tests; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005243:Feces / Q000737:chemistry; D005561:Formates / Q000097:blood; D006801:Humans; D000432:Methanol / Q000506:poisoning; D009566:Nitrates / Q000032:analysis; D009573:Nitrites / Q000032:analysis
chemical_list: D005561:Formates; D009566:Nitrates; D009573:Nitrites; C030544:formic acid; D000432:Methanol
keywords: Contactless conductivity detection; Exhaled breath condensate; Low sample volume; Methanol intoxication; Portable capillary electrophoresis