title: Role of NSC319726 in ovarian cancer based on the bioinformatics analyses.
abstract: OBJECTIVE
  This study aimed to explore the molecular mechanisms of NSC319726 in ovarian cancer by bioinformatics analyses.
  
  
  METHODS
  Gene expression profile GSE35972 was downloaded from the Gene Expression Omnibus database. The data set contains six samples, including three samples of TOV112D cells untreated and three samples of TOV112D cells treated with NSC319726. The differentially expressed genes (DEGs) between untreated and treated samples were analyzed using the limma package. Kyoto Encyclopedia of Genes and Genomes pathway analysis was performed using the signaling pathway impact analysis package, followed by the functional annotation of DEGs and protein-protein interaction network construction. Finally, the subnetwork was identified, and Gene Ontology functional enrichment analysis was performed on the DEGs enriched in the subnetwork.
  
  
  RESULTS
  A total of 120 upregulated and 126 downregulated DEGs were identified. Six Kyoto Encyclopedia of Genes and Genomes pathways were significantly perturbed by DEGs, and the pathway of oocyte meiosis was identified as the most perturbed one. Oocyte meiosis was enriched by eight downregulated DEGs, such as ribosomal protein S6 kinase, 90 kDa, and polypeptide 6 (RPS6KA6). After functional annotation, eight transcription factors were upregulated (such as B-cell CLL/lymphoma 6 [BCL6]), and three transcription factors were downregulated. Seven tumor suppressor genes, such as forkhead box O3 (FOXO3), were upregulated. Additionally, in the protein-protein interaction network and subnetwork, cyclin B1 (CCNB1) and cell division cycle 20 (CDC20) were hub genes, which were also involved in the functions related to mitotic cell cycle.
  
  
  CONCLUSIONS
  NSC319726 may play an efficient role against ovarian cancer via targeting genes, such as RPS6KA6, BCL6, FOXO3, CCNB1, and CDC20, which are involved in oocyte meiosis pathway.
authors.affiliation: Department of Chinese Medicine, The Second Hospital of Jilin University, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Chinese Medicine, The Second Hospital of Jilin University, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Chinese Medicine, The Second Hospital of Jilin University, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
authors.affiliation: The Clinical Medical College of Jilin University (Grade 2013), People's Republic of China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Chinese Medicine, China-Japan Union Hospital of Jilin University, Changchun City, People's Republic of China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Wang
keywords: NSC319726; differentially expressed genes; ovarian cancer; pathway impact analysis; protein-protein interaction