title: FGF-21: promising biomarker for detecting ketosis in dairy cows.
abstract: The objective of this study was to investigate the measurement of serum fibroblast growth factor-21 (FGF-21), a protein mainly synthesized by the liver, as a sensitive biomarker for diagnosis of ketosis in dairy cows. Ninety Holstein-Friesian dairy cows (60 healthy and 30 ketosis cases) were selected and divided into a Ketosis group (K), and a Control group (C). We measured serum FGF-21 and other biochemical parameters by commercial ELISA kits. In a combined population of all 90 cows, we found that serum FGF-21 level was lower (P < 0.001) in cows suffering from ketosis. When the β-hydroxybutyric acid (BHBA) level increased over 1.2 mmol/L, the FGF-21 level tended to decline below 300.85 pg/ml. The area under the receiver operating characteristic curve (AUC-ROC) for serum FGF-21 for diagnosis of fatty liver was 0.952-0.025 [95% confidence interval (CI) 0.904, 1.000] which was higher than the AUC-ROC for glucose (Glc) and other tested parameters. We concluded that FGF-21 could be a diagnostic parameter in the evaluation and auxiliary diagnosis of changes in the energy metabolism state, and serum FGF-21 measurement would have a considerable clinical impact and lead to greater profitability in the dairy industry.
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China. xuchuang7175@163.com.
authors.forename: Chuang
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.|Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xia
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Kuilin
authors.initials: K
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Taiyu
authors.initials: T
authors.lastname: Shen
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D005260:Female; D005346:Fibroblast Growth Factors / Q000097:blood*; D007662:Ketosis / Q000097:blood / Q000662:veterinary*; D012372:ROC Curve
chemical_list: D015415:Biomarkers; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors
keywords: Blood metabolites; Diagnosis; Ketosis; Serum FGF-21