title: DNA Methylation mediated down-regulating of MicroRNA-33b and its role in gastric cancer.
abstract: The discovery of microRNAs (miRNAs) provides a new and powerful tool for studying the mechanism, diagnosis and treatment of human cancers. Currently, down-regulation of tumor suppressive miRNAs by CpG island hypermethylation is emerging as a common hallmark of cancer. Here, we reported that the down-regulation of miR-33b was associated with pM stage of gastric cancer (GC) patients. Ectopic expression of miR-33b in HGC-27 and MGC-803 cells inhibited cell proliferation, migration and invasion, which might be due to miR-33b targeting oncogene c-Myc. Moreover, enhanced methylation level of the CpG island upstream of miR-33b in GC patients with down-regulated miR-33b was confirmed by methylation-specific PCR (MSP) amplification. Furthermore, re-introduction of miR-33b significantly suppressed tumorigenesis of GC cells in the nude mice. In conclusion, miR-33b acts as a tumor suppressor and hypermethylation of the CpG island upstream of miR-33b is responsible for its down-regulation in gastric cancer.
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.|Department of Surgery, The Shanxi Academy of Medical Sciences &Shanxi Dayi Hospital, Taiyuan 030032, PR China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Yin
authors.affiliation: The First Surgery Department of Nanlou, Chinese People's Liberation Army General Hospital, Beijing 100853, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Su
authors.affiliation: Department of Clinical Laboratory, Beijing Shijitan Hospital, Beijing100038, PR China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.|Department of Surgery, The Shanxi Academy of Medical Sciences &Shanxi Dayi Hospital, Taiyuan 030032, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Beijing Shijitan Hospital, Beijing100038, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Gong
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Changzheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Junwu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, CAMS &PUMC, Beijing 100005, PR China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Beijing 100005, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018899:CpG Islands; D019175:DNA Methylation; D004195:Disease Models, Animal; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D047368:Tumor Burden
chemical_list: C550416:MIRN33a microRNA, human; D035683:MicroRNAs