title: [Research for the antiapoptotic mechanisms of bone marrow mesenchymal stem cells in the treatment of brain ischemia stroke].
abstract: OBJECTIVE
  To evaluate the anti-apoptosis role of bone marrow mesenchymal stem cells (BMMCs) transplantation to cell cerebral brain ischemia mice.
  
  
  METHODS
  BMMCs were separated through Ficoll from bone marrow, after amplified in vitro, flow cytometry was used to identify the surface markers. Then cells were transplanted into Middle cerebral artery occlusion (MACO) mice, in situ cell death detection kit and Western blot were used to examine the cell apoptosis. Immunofluorescence and Western blot were used to detect the expression of eNOS, ICAM-1, CD31 which are related to the repair of vascular endothelial cells.
  
  
  RESULTS
  After BMMCs transplantation, the number of apoptosis cells was decreased from (78.2±1.4) to (12.8±3.0), P<0.05. The expression of eNOS (80.0±6.2 vs 31.2±1.6, P<0.01) and CD31 (85±3 vs 45±5, P<0.01), were higher than MACO, while ICAM-1 was lower than MACO (34.1±2.2 vs 85.2±2.8, P<0.01).
  
  
  CONCLUSIONS
  BMMCs reduce the cell apoptosis of ischemia mice through regulate the expression of vascular endothelial cells relate genes.
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China; Email: dhuyamei2013@163.com.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China.
authors.forename: Shujian
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China.
authors.forename: Qianlin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Neurology, People's Hospital, Zhengzhou University; Henan Provical People's Hospital, Zhengzhou 450003, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D001921:Brain; D002545:Brain Ischemia; D042783:Endothelial Cells; D006412:Hematopoietic Stem Cells; D020244:Infarction, Middle Cerebral Artery; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D020521:Stroke