title: SATB2 is localized to the centrosome and spindle maintenance and its knockdown leads to downregulation of CDK2.
abstract: Though special AT-rich sequence-binding protein 2 (SATB2) is reported as a transcriptional regulator of skeletal development and osteogenic differentiation, the underlying mechanism of SATB2 is not fully understood. Herein, we report that SATB2 is localized to the mitotic microtubules, the centrosome, and midbodies in mitotic cells with alpha-tubulin. Moreover, siRNA-mediated disruption of SATB2 in H460 cells caused the defect of nuclear morphology and multinucleate cells. SATB2 siRNA knockdown reduced the viability and downregulated the CDK2 expression in SKOV3 cells. Consistently, cell cycle analysis demonstrated that the silencing of SATB2 induced cell-cycle G1 arrest. Furthermore, proteosomal inhibitor MG132 treatment rescued the downregulation of CDK2 in SATB2-silenced SKOV3 cells. Taken together, our findings suggest that SATB2 regulates the mitosis of cell cycle and affects G1 cell cycle via interaction with CDK2.
authors.affiliation: College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Eun Ah
authors.initials: EA
authors.lastname: Shin
authors.affiliation: College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Sohn
authors.affiliation: College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Gunho
authors.initials: G
authors.lastname: Won
authors.affiliation: College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Sangwook
authors.initials: S
authors.lastname: Yun
authors.affiliation: College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. sungkim7@khu.ac.kr.
authors.forename: Sung-hoon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D002452:Cell Count; D045744:Cell Line, Tumor; D053144:Cell Nucleus Shape / Q000187:drug effects; D018385:Centrosome / Q000187:drug effects / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D055785:Gene Knockdown Techniques; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D007976:Leupeptins; D036961:Matrix Attachment Region Binding Proteins; C521297:SATB2 protein, human; D014157:Transcription Factors; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde
keywords: CDK2; Cytokinesis; Special AT-rich binding protein 2 (SATB2)