title: A quadruplet electrochemical platform for ultrasensitive and simultaneous detection of ascorbic acid, dopamine, uric acid and acetaminophen based on a ferrocene derivative functional Au NPs/carbon dots nanocomposite and graphene.
abstract: In this work, a new nanomaterial of thiol functional ferrocene derivative (Fc-SH) stabilized Au NPs/carbon dots nanocomposite (Au/C NC) coupling with graphene modified glassy carbon electrode (Fc-S-Au/C NC/graphene/GCE) was fabricated to serve as a quadruplet detection platform for ultrasensitive and simultaneous determination of ascorbic acid (AA), dopamine (DA), uric acid (UA) and acetaminophen (AC). The Au/C NC was synthesized by adding HAuCl4 into carbon nanodots solution without using any additional reductant and stabilizing agent. Then the Fc-SH was utilized as the protective and capping agent to modify the Au/C NC. Transmission electron microscopy (TEM), UV-Vis, Fourier-transform infrared (FT-IR), scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) were adopted to characterize the morphology and electrochemical properties of the materials and the electrodes. The Fc-S-Au/C NC/graphene/GCE exhibits a synergistic catalytic and amplification effects towards oxidation of AA, DA, UA and AC owing to the existence of the nanomaterial and electron mediator. When simultaneous detection of AA, DA, UA and AC, the oxidation peak potentials of the four compounds on the electrode can be well separated and the peak currents were linearly dependent on their concentrations. The quadruplet detection platform shows excellent linear range and ultrasensitive response to the four components, the detection limits were estimated to be as low as 1.00, 0.05, 0.12 and 0.10 μM (S/N = 3), and the modified electrode exhibits excellent stability and reproducibility. The proposed electrode has been successfully applied to detect of these four analytes in real samples with satisfactory results.
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Huang
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Qiujun
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: liumeilingww@126.com.
authors.forename: Meiling
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000082:Acetaminophen / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D004298:Dopamine / Q000032:analysis*; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D000075163:Metallocenes; D053761:Nanocomposites; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000032:analysis*
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D014527:Uric Acid; D000082:Acetaminophen; D001205:Ascorbic Acid; C004998:ferrocene; D004298:Dopamine
keywords: Au/C NC; Graphene; Quadruplet detection; Signal amplification; Simultaneous determination