title: [Protective effects of Purα on rat hippocampus DNA damage induced by epilepsy].
abstract: OBJECTIVE
  To investigate the protective effects of Purα protein on rat hippocampus DNA damage induced by epilepsy and the effects of Purα protein on the repair of DNA damage.
  
  
  METHODS
  The Purα overexpressing and siRNA lentiviruses were packaged in vitro and the high tittered virion was injected into rat hippocampus guided by stereotaxic apparatus. 14 days later after the lentivirus injection, epileptic onset is induced by intraperitoneal injection of pilocarpine. The experimental animals were executed 1 hour after the epileptic onset and the hippocampus samples were collected for immunohistochemical staining and Western blotting assay was used to examine the pertinent protein expression to investigate the protective effects of Purα on DNA damage and repair.
  
  
  RESULTS
  Immunohistochemical analysis demonstrated that γH2AX, a signal protein of DNA damage, expressed in rat hippocampal CA1 region. 20 slides of rat hippocampal CA1 region with the same background and position were chosen for γH2AX positive staining cell analysis with image analysis software iPP6.0 and the cells with positive staining were selected to evaluate the average optical density. Compared with empty vector group (0.40 ± 0.11) and control group (0.42 ± 0.05), the number of positive staining cells in Purα overexpression group (0.15 ± 0.05) was significantly decreased (P < 0.01), while the number increased in Purα silence group (0.68 ± 0.06) (P < 0.01). Western blotting analysis showed, compared with empty vector group (0.93 ± 0.11) and control group (1.00 ± 0.00), that the expression levels of proteins related to the DNA repair such as parp-1, was much lower in Purα overexpression group (0.17 ± 0.09), while it increased in Purα silence group (P < 0.01).
  
  
  CONCLUSIONS
  The DNA damage can occur in the early stage of epilepsy onset, and Purα protein can protect the DNA damage caused by epilepsy and also participate in the repair process of DNA damage.
authors.affiliation: Department of Neurosurgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Email: suntao@nxmu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yang
authors.forename: Zuyao
authors.initials: Z
authors.lastname: Song
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Niu
authors.forename: Jianqi
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins; D004827:Epilepsy; D006624:Hippocampus; D010862:Pilocarpine; D051381:Rats; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D014157:Transcription Factors; D010862:Pilocarpine