title: Assessment of in vitro genotoxic and cytotoxic effects of flurbiprofen on human cultured lymphocytes.
abstract: Flurbiprofen is non-steroidal anti-inflammatory drug which is commonly used for its analgesic, antipyretic, and anti-inflammatory effects. The purpose of the study was to explore the genotoxic and cytotoxic effects of flurbiprofen in human cultured lymphocytes by sister chromatid exchange, chromosome aberration, and cytokinesis-blocked micronucleus tests. 10, 20, 30, and 40 μg/mL concentrations of flurbiprofen (solvent is DMSO) were used to treatment of human cultured lymphocytes at two different treatment periods (24 and 48 h). Flurbiprofen had no significant genotoxic effect in any of these tests. But exposing to flurbiprofen for 24 and 48 h led to significant decrease on proliferation index, mitotic index, and nuclear division index (NDI). Also, all decreases were concentration-dependent (except NDI at 24 h treatment period). Consequently, the findings of this research showed that flurbiprofen had cytotoxic effects in human blood lymphocytes.
authors.affiliation: a Department of Biology , Institute of Science, Cukurova University , Adana , Turkey .
authors.forename: Taygun
authors.initials: T
authors.lastname: Timocin
authors.affiliation: b Department of Biology , Faculty of Science and Letters, Cukurova University , Adana , Turkey , and.
authors.forename: Hasan Basri
authors.initials: HB
authors.lastname: Ila
authors.affiliation: c Department of Biotechnology , Institute of Science, Cukurova University , Adana , Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Dordu
authors.affiliation: a Department of Biology , Institute of Science, Cukurova University , Adana , Turkey .
authors.forename: Mehmet Tahir
authors.initials: MT
authors.lastname: Husunet
authors.affiliation: c Department of Biotechnology , Institute of Science, Cukurova University , Adana , Turkey.
authors.forename: Mostafa Norizadeh
authors.initials: MN
authors.lastname: Tazehkand
authors.affiliation: c Department of Biotechnology , Institute of Science, Cukurova University , Adana , Turkey.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Valipour
authors.affiliation: b Department of Biology , Faculty of Science and Letters, Cukurova University , Adana , Turkey , and.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Topaktas
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced*; D005260:Female; D005480:Flurbiprofen / Q000633:toxicity*; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D008940:Mitotic Index; D009152:Mutagenicity Tests; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005480:Flurbiprofen
keywords: CA; Flurbiprofen; MN; SCE; cytotoxicity; human cultured lymphocytes