title: Alternative energy production pathways in Taenia crassiceps cysticerci in vitro exposed to a benzimidazole derivative (RCB20).
abstract: Biochemical studies of benzimidazole derivatives are important to determine their mode of action and activity against parasites. The lack of antihelminthic alternatives to treat parasitic infections and albendazole resistance cases make the search for new antiparasitary drugs of utmost importance. The 6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative with promising effect. This study evaluated the effect of different concentrations of RCB20 in the alternative energetic pathway of in vitro Taenia crassiceps cysticerci. The parasites were in vitro exposed to 6.5 and 13 µM of RCB20 and albendazole sulfoxide (ABZSO). The quantification of acetate, acetoacetate, β-hydroxybutyrate, fumarate and propionate was performed by high-performance liquid chromatography. The quantification of urea, creatinine and total proteins was performed by spectrophotometry. The increase in β-hydroxybutyrate reflects the enhancement of the fatty acid oxidation in the treated groups. Volatile fatty acids secretion, acetate and propionate, was increased in the treated groups. The secretion mechanisms of the treated parasites were impaired due to organic acids increased concentrations in the cysticerci. It is possible to conclude that the metabolic effect on alternative energetic pathways is slightly increased in the parasites treated with RCB20 than the ones treated with ABZSO.
authors.affiliation: Laboratory of Studies of the Host-Parasite Relationship,Tropical Pathology and Public Health Institute,Federal University of Goias.
authors.forename: Carolina Miguel
authors.initials: CM
authors.lastname: Fraga
authors.affiliation: Laboratory of Studies of the Host-Parasite Relationship,Tropical Pathology and Public Health Institute,Federal University of Goias.
authors.forename: Tatiane Luiza
authors.initials: TL
authors.lastname: Da Costa
authors.affiliation: Laboratory of Studies of the Host-Parasite Relationship,Tropical Pathology and Public Health Institute,Federal University of Goias.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: De Castro
authors.affiliation: Laboratorio de Biologia del Citoesqueleto,Departamento de Microbiologia y Parasitologia,Facultad de Medicina,Universidad Nacional Autonoma de Mexico (UNAM),México,DF 04510,Mexico.
authors.forename: Olivia
authors.initials: O
authors.lastname: Reynoso-Ducoing
authors.affiliation: Laboratorio de Biologia del Citoesqueleto,Departamento de Microbiologia y Parasitologia,Facultad de Medicina,Universidad Nacional Autonoma de Mexico (UNAM),México,DF 04510,Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Ambrosio
authors.affiliation: Facultad de Química,Departamento de Farmacia,Universidad Nacional Autónoma de México (UNAM),México,DF 04510,Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Hernández-Campos
authors.affiliation: Facultad de Química,Departamento de Farmacia,Universidad Nacional Autónoma de México (UNAM),México,DF 04510,Mexico.
authors.forename: Rafael
authors.initials: R
authors.lastname: Castillo
authors.affiliation: Laboratory of Studies of the Host-Parasite Relationship,Tropical Pathology and Public Health Institute,Federal University of Goias.
authors.forename: Marina Clare
authors.initials: MC
authors.lastname: Vinaud
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D000090:Acetoacetates / Q000378:metabolism; D015766:Albendazole / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000923:Anticestodal Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D003404:Creatinine / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D003552:Cysticercus / Q000187:drug effects* / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005650:Fumarates / Q000032:analysis; D051379:Mice; D011422:Propionates / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D013621:Taenia / Q000187:drug effects / Q000378:metabolism; D014508:Urea / Q000032:analysis
chemical_list: C557917:6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole; D000090:Acetoacetates; D000923:Anticestodal Agents; D001562:Benzimidazoles; D003470:Culture Media; D005650:Fumarates; D011422:Propionates; D011506:Proteins; C016635:acetoacetic acid; D014508:Urea; D003404:Creatinine; D015766:Albendazole; C027186:albendazole sulfoxide; D020155:3-Hydroxybutyric Acid
keywords: Taenia crassiceps; benzimidazole derivative; fatty acids oxidation; ketonic bodies; protein catabolism