title: In-vitro microbial production of conjugated linoleic acid by probiotic L. plantarum strains: Utilization as a functional starter culture in sucuk fermentation.
abstract: Twenty-three probiotic Lactobacillus plantarum strains were screened in-vitro to determine their ability to produce conjugated linoleic acid (CLA). L. plantarum AA1-2 and L. plantarum AB20-961 were identified as potential strains for CLA production. Optimum conditions for these strains to produce high levels of CLA were determined by evaluating the amount of added hydrolyzed sunflower oil (HSO) and initial pH levels in a nutrient medium. The highest CLA production was obtained in medium with pH6.0 and 2% HSO (P<0.05). Those strains were then used as starter culture in sucuk fermentation. Five sucuk treatments included a control (no starter culture), two sucuk groups with L. plantarum AA1-2 at the initial pH of 5.8 or 6.0 and two sucuk groups with L. plantarum AB20-961 at the initial pH of 5.8 or 6.0. Results indicate that L. plantarum AB20-961 produced higher amount of CLA in sucuk at initial pH of 5.8 and 6.0 levels during first 24h of fermentation compared with other groups. CLA isomer concentration decreased in all sucuk groups during the rest of the fermentation period (P<0.05) and remained quite stable during the storage. This study demonstrated that probiotic L. plantarum AB20-961 can be used in sucuk manufacturing without posing any quality problems.
authors.affiliation: Nevsehir Hacı Bektas Veli University, Faculty of Engineering and Architecture, Department of Food Engineering, 50300 Nevsehir, Turkey. Electronic address: cemokanozer@nevsehir.edu.tr.
authors.forename: Cem O
authors.initials: CO
authors.lastname: Özer
authors.affiliation: Suleyman Demirel University, Faculty of Engineering, Department of Food Engineering, 32260 Isparta, Turkey.
authors.forename: Birol
authors.initials: B
authors.lastname: Kılıç
authors.affiliation: Mehmet Akif Ersoy University, Faculty of Engineering-Architecture, Department of Food Engineering, 15030 Burdur, Turkey.
authors.forename: Gülden Başyiğit
authors.initials: GB
authors.lastname: Kılıç
mesh_terms: D000818:Animals; D002417:Cattle; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum; D044243:Linoleic Acids, Conjugated / Q000378:metabolism*; D008461:Meat Products / Q000032:analysis* / Q000382:microbiology; D010938:Plant Oils; D019936:Probiotics; D013045:Species Specificity; D000074242:Sunflower Oil
chemical_list: D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D000074242:Sunflower Oil
keywords: Conjugated linoleic acid; Lactobacillus plantarum; Probiotic; Sucuk