title: The effect of insulin on plasma glucose concentrations, expression of hepatic glucose transporters and key gluconeogenic enzymes during the perinatal period in broiler chickens.
abstract: Chickens have blood glucose concentrations that are twofold higher than those observed in mammals. Moreover, the insulin sensitivity seems to decrease with postnatal age in both broiler and layer chickens. However, little is known about the response of insulin on plasma glucose concentrations and mRNA abundance of hepatic glucose transporters 1, 2, 3, 8, 9 and 12 (GLUT1, 2, 3, 8, 9 and 12) and three regulatory enzymes of the gluconeogenesis, phosphoenolpyruvate carboxykinase 1 and 2 (PCK1 and 2) or fructose-1,6-biphosphatase 1 (FBP1) in chicks during the perinatal period. In the present study, broiler embryos on embryonic day (ED)16, ED18 or newly-hatched broiler chicks were injected intravenously with bovine insulin (1μg/g body weight (BW)) to examine plasma glucose response and changes in hepatic mRNA abundance of the GLUTs, PCK1 and 2 and FBP1. Results were compared with a non-treated control group and a saline-injected sham group. Plasma glucose levels of insulin-treated ED18 embryos recovered faster from their minimum level than those of insulin-treated ED16 embryos or newly-hatched chicks. In addition, at the minimum plasma glucose level seven hours post-injection (PI), hepatic GLUT2, FBP1 and PCK2 mRNA abundance was decreased in insulin-injected embryos, compared to sham and control groups, being most pronounced when insulin injection occurred on ED16.
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium.
authors.forename: Lies
authors.initials: L
authors.lastname: Franssens
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium.
authors.forename: Jens
authors.initials: J
authors.lastname: Lesuisse
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: Willems
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium.
authors.forename: Hilke
authors.initials: H
authors.lastname: Willemsen
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium; ILVO Animal Sciences Unit, Scheldeweg 68, 9090 Melle, Belgium.
authors.forename: Astrid
authors.initials: A
authors.lastname: Koppenol
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, 330045 Jiangxi, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Guo
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium. Electronic address: johan.buyse@biw.kuleuven.be.
authors.forename: Johan
authors.initials: J
authors.lastname: Buyse
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium.
authors.forename: Eddy
authors.initials: E
authors.lastname: Decuypere
authors.affiliation: KU Leuven, Department of Biosystems, Laboratory of Livestock Physiology, Kasteelpark Arenberg 30, Box 2456, 3001 Leuven, Belgium; University of Liège, Gembloux Agro-Bio Tech, Animal Science Unit, Passage des Déportés 2, 5030 Gembloux, Belgium.
authors.forename: Nadia
authors.initials: N
authors.lastname: Everaert
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology; D005943:Gluconeogenesis / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051247:Sodium-Glucose Transport Proteins / Q000378:metabolism*
chemical_list: D007328:Insulin; D051247:Sodium-Glucose Transport Proteins; D005947:Glucose
keywords: Embryonic development; Glucose transporter; Hypoglycemia; Insulin; Regulatory enzymes of gluconeogenesis