title: Biomolecular implementation of a quasi sliding mode feedback controller based on DNA strand displacement reactions.
abstract: A fundamental aim of synthetic biology is to achieve the capability to design and implement robust embedded biomolecular feedback control circuits. An approach to realize this objective is to use abstract chemical reaction networks (CRNs) as a programming language for the design of complex circuits and networks. Here, we employ this approach to facilitate the implementation of a class of nonlinear feedback controllers based on sliding mode control theory. We show how a set of two-step irreversible reactions with ultrasensitive response dynamics can provide a biomolecular implementation of a nonlinear quasi sliding mode (QSM) controller. We implement our controller in closed-loop with a prototype of a biological pathway and demonstrate that the nonlinear QSM controller outperforms a traditional linear controller by facilitating faster tracking response dynamics without introducing overshoots in the transient response.
authors.forename: Rucha
authors.initials: R
authors.lastname: Sawlekar
authors.forename: Francesco
authors.initials: F
authors.lastname: Montefusco
authors.forename: Vishwesh
authors.initials: V
authors.lastname: Kulkarni
authors.forename: Declan G
authors.initials: DG
authors.lastname: Bates
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D005246:Feedback; D017711:Nonlinear Dynamics; D058615:Synthetic Biology
chemical_list: D004247:DNA