title: Performance and Verification of a Real-Time PCR Assay Targeting the gyrA Gene for Prediction of Ciprofloxacin Resistance in Neisseria gonorrhoeae.
abstract: In the United States, 19.2% of Neisseria gonorrhoeae isolates are resistant to ciprofloxacin. We evaluated a real-time PCR assay to predict ciprofloxacin susceptibility using residual DNA from the Roche Cobas 4800 CT/NG assay. The results of the assay were 100% concordant with agar dilution susceptibility test results for 100 clinical isolates. Among 76 clinical urine and swab specimens positive for N. gonorrhoeae by the Cobas assay, 71% could be genotyped. The test took 1.5 h to perform, allowing the physician to receive results in time to make informed clinical decisions.
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
authors.forename: P
authors.initials: P
authors.lastname: Hemarajata
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.affiliation: Neisseria Reference Laboratory, GISP Regional Laboratory, University of Washington Harborview Medical Center, Seattle, Washington, USA.
authors.forename: O O
authors.initials: OO
authors.lastname: Soge
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA rhumphries@mednet.ucla.edu.
authors.forename: R M
authors.initials: RM
authors.lastname: Humphries
authors.affiliation: Department of Medicine, Division of Infectious Diseases and Department of Epidemiology, David Geffen School of Medicine and Fielding School of Public Health, University of California, Los Angeles, California, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Klausner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002939:Ciprofloxacin; D027081:DNA Gyrase