title: Anomalous redispersibility behavior of glycerophosphate deyhydrogenase microparticles dried in an acoustic levitator or bench-top spray dryer.
abstract: The enzyme glycerophosphate dehydrogenase (GPDH) behaves differently when dried either as single droplets in an acoustic levitator or spray dried on a bench-top machine. The GPDH in particles dried in the levitator at a drying gas temperature of 60°C could not be redispersed in water, whereas spray drying at an outlet temperature of 92°C produced denaturation but the particles were redissolvable. One difference between the two processes is that the larger levitated droplets take longer to dry than the small spray dried droplets. The slow drying process of the levitated droplet/particle apparently causes denaturation that is sufficient to make the particles non-redispersible. This does not happen on spray drying.
authors.affiliation: Division of Pharmaceutics, Friedrich-Alexander University, Erlangen, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Lorenzen
authors.affiliation: Division of Pharmaceutics, Friedrich-Alexander University, Erlangen, Germany. Electronic address: geoff.lee@fau.de.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Lee
mesh_terms: D000162:Acoustics; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003890:Desiccation / Q000379:methods*; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis / Q000737:chemistry*; D008863:Microspheres
chemical_list: D005993:Glycerolphosphate Dehydrogenase
keywords: Acoustic levitator; Denature; Protein; Solubility; Spray dried