title: Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis.
abstract: Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.
authors.affiliation: Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom Wellcome Trust Liverpool Glasgow Centre for Global Health Research, Liverpool, United Kingdom Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa jscriven@liv.ac.uk.
authors.forename: James E
authors.initials: JE
authors.lastname: Scriven
authors.affiliation: Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Graham
authors.affiliation: Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Schutz
authors.affiliation: South African TB Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine and Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Scriba
authors.affiliation: Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa Department of Medicine, Imperial College, London, United Kingdom Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wilkinson
authors.affiliation: Department of Medicine, University of Minnesota, Minnesota, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Boulware
authors.affiliation: Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa Department of Medicine, Imperial College, London, United Kingdom.
authors.forename: Graeme
authors.initials: G
authors.lastname: Meintjes
authors.affiliation: Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom Wellcome Trust Liverpool Glasgow Centre for Global Health Research, Liverpool, United Kingdom.
authors.forename: David G
authors.initials: DG
authors.lastname: Lalloo
authors.affiliation: Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom.
authors.forename: Britta C
authors.initials: BC
authors.lastname: Urban
mesh_terms: D002555:Cerebrospinal Fluid / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016919:Meningitis, Cryptococcal / Q000175:diagnosis* / Q000382:microbiology*