title: Laser processing of electrospun PCL fiber mats for tissue engineering.
abstract: OBJECTIVE
  Processing technologies for cutting and joining electrospun fiber mats are required to produce complex three-dimensional (3D) structures, like a scaffold for heart valve tissue engineering. The ability to bond very thin porous sheets, thus forming a stable 3D geometry, offers completely new design strategies such as organ-shaped scaffolds with void chambers inside. In this study, solvent, glue and laser bonding are compared with regard to their retention force and practicability.
  
  
  METHODS
  For this purpose, samples were prepared by applying each bonding technique. In addition, two different ways of preparing the bonding site were investigated: a portion of mats were bonded by an L-joint and the others by a T-joint; then tensile testing was performed until tearing of the bonding occurred. Additionally, the edges of laser cut fiber mats were investigated in order to evaluate the influence of thermal effects of the laser beam and ongoing changes in pore structure.
  
  
  RESULTS
  It was found that laser cut fiber mats were slightly deformed due to thermal effects, but still had an open, porous structure at the site of the cut. Results also show that joining of fiber mats by laser led to the best bonding result with highest retention force. Application of solvent and glue led to a nonuniform and noncontinuous bonding with lower retention forces.
  
  
  CONCLUSIONS
  A first proof of concept for a heart valve-shaped scaffold was created by laser bonding. Thus, the laser is an advantageous tool for post-processing fiber mats and produce complex 3D structures for different applications.
authors.forename: Holger
authors.initials: H
authors.lastname: Zernetsch
authors.forename: Alexander
authors.initials: A
authors.lastname: Kern
authors.forename: Peter
authors.initials: P
authors.lastname: Jäschke
authors.forename: Birgit
authors.initials: B
authors.lastname: Glasmacher
mesh_terms: D001672:Biocompatible Materials; D011091:Polyesters; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters