title: CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro.
abstract: The protein CD24 is a cell surface protein that appears to function as an adhesion molecule; its expression has been shown to correlate with prognosis in a variety of tumors. Herein, we investigated the possible role and mechanism of CD24 in cervical cancer. Our results showed that CD24 was overexpressed in cervical cancer tissues compared with that in the adjacent non‑cancerous tissues by qPCR, immunohistochemistry and western blotting technologies. To explore the possible mechanism of CD24 in cervical cancer, we elucidated the effect of CD24 on the proliferation and apoptosis of cervical cancer HeLa cells and found that a considerable increase in cell proliferation was observed in the HeLa cells with CD24 overexpession. The rate of cell apoptosis was decreased in the HeLa/CD24 cells compared with the HeLa or HeLa/vector cells. Cell apoptosis is closely related with a reduction in mitochondrial membrane potential (ΔΨm) and an increase in intracellular reactive oxygen species (ROS) and calcium ion (Ca2+) concentrations. Our results showed that overexpression of CD24 in the cervical cancer HeLa cells, led to an increase in ΔΨm and a decrease in intracellular ROS and Ca2+ concentrations. Furthermore, we found that CD24 was correlated with dysregulation of the MAPK signaling pathway in cervical cancer tissues in vitro. At the same time, we found that CD24 overexpression affected the expression of p38, JNK2 and c-Jun in vitro. In summary, our results suggest that CD24 is upregulated in cervical cancer tissues and plays its functions by affecting the MAPK signaling pathway in cervical cancer.
authors.affiliation: Hunan Provincial Tumor Hospital and The Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410006, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Pei
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Guangchao
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Histology and Embryology, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Huo
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liao
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Junyu
authors.initials: J
authors.lastname: He
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Long
authors.affiliation: Research Center of Carcinogenesis and Targeted Therapy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.affiliation: Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Songshu
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.affiliation: Hunan Provincial Tumor Hospital and The Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410006, P.R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chen
authors.affiliation: Hunan Provincial Tumor Hospital and The Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410006, P.R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.affiliation: Hunan Provincial Tumor Hospital and The Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410006, P.R. China.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
authors.affiliation: Hunan Provincial Tumor Hospital and The Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410006, P.R. China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051927:CD24 Antigen / Q000096:biosynthesis* / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000473:pathology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D051927:CD24 Antigen; D017382:Reactive Oxygen Species; D020929:Mitogen-Activated Protein Kinase Kinases