title: Methods for in vitro functional analysis of iPSC derived cardiomyocytes - Special focus on analyzing the mechanical beating behavior.
abstract: A rapidly increasing number of papers describing novel iPSC models for cardiac diseases are being published. To be able to understand the disease mechanisms in more detail, we should also take the full advantage of the various methods for analyzing these cell models. The traditionally and commonly used electrophysiological analysis methods have been recently accompanied by novel approaches for analyzing the mechanical beatingbehavior of the cardiomyocytes. In this review, we provide first a concise overview on the methodology for cardiomyocyte functional analysis and then concentrate on the video microscopy, which provides a promise for a new faster yet reliable method for cardiomyocyte functional analysis. We also show how analysis conditions may affect the results. Development of the methodology not only serves the basic research on the disease models, but could also provide the much needed efficient early phase screening method for cardiac safety toxicology. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.
authors.affiliation: University of Tampere, BioMediTech and School of Medicine, Tampere, Finland. Electronic address: eeva.laurila@uta.fi.
authors.forename: Eeva
authors.initials: E
authors.lastname: Laurila
authors.affiliation: Tampere University of Technology, Department of Electronics and Communications Engineering, BioMediTech, Tampere, Finland.
authors.forename: Antti
authors.initials: A
authors.lastname: Ahola
authors.affiliation: Tampere University of Technology, Department of Electronics and Communications Engineering, BioMediTech, Tampere, Finland.
authors.forename: Jari
authors.initials: J
authors.lastname: Hyttinen
authors.affiliation: University of Tampere, BioMediTech and School of Medicine, Tampere, Finland; Heart Hospital, Tampere University Hospital, Tampere, Finland.
authors.forename: Katriina
authors.initials: K
authors.lastname: Aalto-Setälä
mesh_terms: D000200:Action Potentials; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D006339:Heart Rate; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000502:physiology*; D018715:Microscopy, Video / Q000379:methods* / Q000592:standards; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
keywords: Disease modeling; Induced pluripotent stem cells; Safety toxicology; Stem cell derived cardiomyocytes; Video microscopy