title: Oxidative status and lymphocyte DNA damage in patients with acute pancreatitis and its relationship with severity of acute pancreatitis.
abstract: OBJECTIVE
  Acute pancreatitis (AP) is a life-threatening disease with a rising incidence. The aim of this study was to investigate the association between oxidative status, lymphocyte deoxyribonucleic acid (DNA) damage, and acute pancreatitis.
  
  
  METHODS
  A total of 45 patients with AP and 35 healthy controls were included in the study. We assessed pancreatic enzymes, oxidative stress, and lymphocyte DNA damage. The severity of AP disease was determined by the Harmless Acute Pancreatitis Score (HAPS) and Balthazar scoring systems.
  
  
  RESULTS
  In AP patients, lymphocyte DNA damage was significantly higher than in controls [49.84±25.48 arbitrary units (AU) vs. 28.80±13.98 AU, p<0.001]. The plasma total oxidative status (TOS) and oxidative stress index (OSI) were higher in patients than in healthy controls (10.36±5.54 vs. 8.47±2.66, p<0.05; 0.64±0.35 vs. 0.45±0.13 AU, p<0.001, respectively). The plasma total antioxidant status level in patients was lower than in healthy controls (1.66±0.19 vs. 1.86±0.18, p<0.001). Lymphocyte DNA damage was correlated with TOS, OSI, and HAPS and Balthazar scores.
  
  
  CONCLUSIONS
  This study shows that patients with AP have higher lymphocyte DNA damage and more deteriorated oxidative status than healthy controls.
authors.affiliation: Department of Emergency Medicine, Bezmialem Vakıf University Faculty of Medicine, İstanbul, Turkey. drokocaman@hotmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Dur
authors.forename: Orhan
authors.initials: O
authors.lastname: Kocaman
authors.forename: Abdurrahim
authors.initials: A
authors.lastname: Koçyiğit
authors.forename: Kenan Ahmet
authors.initials: KA
authors.lastname: Türkdoğan
authors.forename: Ertan
authors.initials: E
authors.lastname: Sönmez
authors.forename: Sıddıka
authors.initials: S
authors.lastname: Keskin
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yiğit
authors.forename: Bedia
authors.initials: B
authors.lastname: Gülen
authors.forename: Elif
authors.initials: E
authors.lastname: Kılıç
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Uysal
mesh_terms: D000208:Acute Disease; D000328:Adult; D000975:Antioxidants / Q000032:analysis; D016022:Case-Control Studies; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics*; D010179:Pancreas / Q000201:enzymology; D010195:Pancreatitis / Q000097:blood* / Q000201:enzymology; D012720:Severity of Illness Index
chemical_list: D000975:Antioxidants