title: Selective disruption of vascular endothelium of zebrafish embryos by ultrafast laser microsurgical treatment.
abstract: In this work, we demonstrate that ultrafast laser irradiation could selectively disrupt vascular endothelium of zebrafish embryos in vivo. Ultrafast lasers minimize the collateral damage in the vicinity of the laser focus and eventually reduce coagulation in the tissues. We have also found that the threshold fluence for lesion formation of the vascular endothelium strongly depends on the developmental stage of the embryos. The threshold laser fluence required to induce apparent lesions in the vascular structure for Somite 14, 20 and 25 stages is about 5 J/cm(2) ~7 J/cm(2), which is much lower than that for the later development stages of Prim 16 and Prim 20 of 30 J/cm(2) ~50 J/cm(2). The proposed method for treating the vascular cord of zebrafish embryos in the early stage of development has potential as a selective and effective method to induce a fatal lesion in the vascular endothelium without damaging the developed blood vessels.
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea.
authors.forename: Suk-Yi
authors.initials: SY
authors.lastname: Woo
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea ; Department of Nano Chemistry, Gacheon University, Seongnam, Gyeonggi-do 461-701, South Korea.
authors.forename: Heh-Young
authors.initials: HY
authors.lastname: Moon
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea.
authors.forename: Tag Gyum
authors.initials: TG
authors.lastname: Kim
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea.
authors.forename: Heung Soon
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea.
authors.forename: Mehra S
authors.initials: MS
authors.lastname: Sidhu
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea ; L2K Co., Ltd.,Yuseong-Gu, Daejeon 305-500, South Korea.
authors.forename: Changho
authors.initials: C
authors.lastname: Kim
authors.affiliation: L2K Co., Ltd.,Yuseong-Gu, Daejeon 305-500, South Korea.
authors.forename: Jae-Phil
authors.initials: JP
authors.lastname: Jeon
authors.affiliation: Division of Advanced Technology, Korea Research Institute of Standards and Science, 1-Doryong-Dong, Yuseong-Gu, Daejeon 305-340, South Korea.
authors.forename: Sae Chae
authors.initials: SC
authors.lastname: Jeoung
keywords: (140.0140) Lasers and laser optics; (170.3890) Medical optics instrumentation; (320.7130) Ultrafast processes in condensed matter, including semiconductors