title: Saccharification of orange peel wastes with crude enzymes from new isolated Aspergillus japonicus PJ01.
abstract: This study investigated the saccharification of orange peel wastes with crude enzymes from Aspergillus japonicus PJ01. Pretreated orange peel powder was hydrolyzed by submerged fermentation (SmF) and solid-state fermentation (SSF) crude enzymes, the results showed that 4 % (w/v) of solid loading, undiluted crude enzymes, and 45 °C were suitable saccharification conditions. The hydrolysis kinetics showed that the apparent Michaelis-Menten constant [Formula: see text] and maximal reaction rate [Formula: see text] were 73.32 g/L and 0.118 g/(L min) for SmF enzyme, and 41.45 g/L and 0.116 g/(L min) for SSF enzyme, respectively. After 48 h of hydrolysis, the saccharification yields were 58.5 and 78.7 %, the reducing sugar concentrations were 14.9 and 20.1 mg/mL by SmF and SSF enzymes. Material balance showed that the SmF enzymatic hydrolysate was enriched galacturonic acid > arabinose > galactose > xylose, and the SSF enzymatic hydrolysate was enriched galacturonic acid > xylose > galactose > arabinose.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
authors.forename: Pei-Jun
authors.initials: PJ
authors.lastname: Li
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China. jlxia@csu.edu.cn.
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Xia
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Nie
authors.affiliation: Hunan Agriculture Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China. Sy6302@sohu.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shan
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D032084:Citrus sinensis / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D062611:Solid Waste
chemical_list: D005656:Fungal Proteins; D062611:Solid Waste
keywords: Enzyme; Kinetic parameter; Orange peel; Saccharification