title: Photo-click construction of a targetable and activatable two-photon probe imaging protease in apoptosis.
abstract: A photo-click reaction was used as an efficient method to construct two-photon fluorescent probes bearing two functional peptides for targeting and for protease cleavage respectively. The activatable two-photon probe constructed by this method was applied to two-photon imaging of caspase-3 both in cellular apoptosis and in tumor tissue.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. njuzy@nju.edu.cn jinboli@nju.edu.cn.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. njuzy@nju.edu.cn jinboli@nju.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. njuzy@nju.edu.cn jinboli@nju.edu.cn.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Joint Laboratory of Green Synthetic Chemistry, University of Science and Technology of China, Hefei 230026, P. R. China.
authors.forename: Qinhua
authors.initials: Q
authors.lastname: Song
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. njuzy@nju.edu.cn jinboli@nju.edu.cn.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. njuzy@nju.edu.cn jinboli@nju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D057930:Click Chemistry; D006367:HeLa Cells; D006801:Humans; D010447:Peptide Hydrolases / Q000627:therapeutic use*; D017785:Photons
chemical_list: D010447:Peptide Hydrolases