title: In vivo HMRS and lipidomic profiling reveals comprehensive changes of hippocampal metabolism during aging in mice.
abstract: Aging is characterized by various cellular changes in the brain. Hippocampus is important for systemic aging and lifespan control. There is still a lack of comprehensive overview of metabolic changes in hippocampus during aging. In this study, we first created an accelerated brain aging mice model through the chronic administration of d-galactose. We then performed a multiplatform metabolomic profiling of mice hippocampus using the combination of in vivo 9.4 T HMRS and in vitro LC-MS/MS based lipidomics. We found N-acetylaspartic acid (NAA), gama-aminobutyric acid (GABA), glutamate/glutamine, taurine, choline, sphingolipids (SMs), phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), phosphatidylglycerols (PGs) and phosphatidylserines (PSs), all of them decreasing with the aging process in mice hippocampus. The changes of sphingolipids and phospholipids were not limited to one single class or molecular species. In contrast, we found the significant accumulation of lactate, myoinositol and phosphatidylcholines (PCs) along with aging in hippocampus. SM (d18:1/20:2), PE (36:2), PG (34:1), PI (36:4), PS (18:0/20:4) and PC (36:0) have the most significant changes along with aging. Network analysis revealed the striking loss of biochemical connectivity and interactions between hippocampal metabolites with aging. The correlation pattern between metabolites in hippocampus could function as biomarkers for aging or diagnosis of aging-related diseases.
authors.affiliation: Department of Nuclear Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong Province, China.
authors.forename: Lejun
authors.initials: L
authors.lastname: Lin
authors.affiliation: Medical Imaging Center, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong Province, China.
authors.forename: Bofeng
authors.initials: B
authors.lastname: Cao
authors.affiliation: Department of Nuclear Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong Province, China. Electronic address: easy0603@163.com.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Medical Imaging Center, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong Province, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Sui
authors.affiliation: Medical Imaging Center, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong Province, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Muping Hospital of Traditional Chinese Medicine, Yantai, 264100, Shandong Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Luan
authors.affiliation: Department of Clinical Laboratory, The Affiliated Hospital of Binzhou Medical University, Yantai, 256603, Shandong Province, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong Province, China.
authors.forename: Shanchun
authors.initials: S
authors.lastname: Li
authors.affiliation: Tianjin SunnyPeak Biotech Co., Ltd, Tianjin, 300057, China. Electronic address: kefengl@sunnypeak.org.
authors.forename: Ke Feng
authors.initials: KF
authors.lastname: Li
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006624:Hippocampus / Q000502:physiology*; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D055442:Metabolome / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018377:Neurotransmitter Agents / Q000378:metabolism*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*
chemical_list: D018377:Neurotransmitter Agents
keywords: 9.4T HMRS; Brain aging; Hippocampus; Lipidomics; Metabolic interactions