title: MicroRNA-140-3p inhibits proliferation, migration and invasion of lung cancer cells by targeting ATP6AP2.
abstract: MicroRNAs are small noncoding RNA molecules that regulate gene expression at the post-transcriptional level. Compelling evidence reveals that there is a causative link between microRNAs deregulation and lung cancer development and metastasis. The aim of present study was to explore the function of miR-140-3p in the development and metastasis of lung cancer cell. Using real-time PCR, we detected the miR-140-3p expression of lung cancer tissues and its pared non-lung cancer tissue. Then, we evaluated the role of miR-140-3p in cell proliferation, invasion and migration using MTT, colony formation assay, Transwell invasion and Transwell migration assay in lung cancer cell lines. As a result, miR-140-3p expression level was lower in lung cancer tissues compared to adjacent normal lung cancer tissue. After miR-140-3p was upregulated in A549 or H1299 cells, cell proliferation, invasion and migration was notably attenuated. Furthermore, we identified ATP6AP2, which is associated with adenosine triphosphatases (ATPases), was a directly target of miR-140-3p in lung cancer cells. In conclusion, our data suggest miR-140-3p/ATP6AP2 axis might act as a potential therapeutic biomarker for lung cancer.
authors.affiliation: Department of Respiratory Diseases, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Kong
authors.affiliation: Department of Oncology, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Ge-Hong
authors.initials: GH
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Yun-Kui
authors.initials: YK
authors.lastname: Huo
authors.affiliation: Department of Respiratory, Shanxi Lvliang City People's Hospital Shanxi, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Diseases, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Cao
authors.affiliation: Department of Respiratory Diseases, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Guo
authors.affiliation: Department of Respiratory Diseases, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Li
authors.affiliation: Department of Respiratory Diseases, Shanxi Medical University First Hospital Shanxi, China.
authors.forename: Xin-Ri
authors.initials: XR
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D000093342:Prorenin Receptor
chemical_list: D035683:MicroRNAs; D011956:Receptors, Cell Surface; D025262:Vacuolar Proton-Translocating ATPases; C458319:ATP6AP2 protein, human; D000093342:Prorenin Receptor; C544302:Mirn140 microRNA, human
keywords: ATP6AP2; Lung cancer; invasion; miR-140-3p; proliferation