title: Paenibacillus puernese sp. nov., a β-glucosidase-producing bacterium isolated from Pu'er tea.
abstract: A Gram-staining-positive, endospore-forming, aerobic, rod-shaped bacterium, designated as DCY97(T), was isolated from ripened Pu'er tea and was identified by using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain DCY97(T) was closely related to Paenibacillus dongdonensis KUDC0114(T) (98.0 %), Paenibacillus oceanisediminis L10(T) (97.7 %), and Paenibacillus barcinonensis BP-23(T) (97.2 %). The phenotypic and chemotaxonomic characteristics of strain DCY97(T) matched with the characteristics of members belonging to the genus Paenibacillus. The major identified polar lipids included phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The predominant quinone was MK-7. The major fatty acids were anteiso-C15:0 (35.1 %), anteiso-C16:0 (19.0 %), and iso-C16:0 (13.9 %). The peptidoglycan cell wall was composed of meso-diaminopimelic acids, alanine, and D-glutamic acid. The genomic DNA G + C content was determined to be 46.7 mol%. The DNA-DNA relatedness between strain DCY97(T) and Paenibacillus dongdonensis KCTC 33221(T), Paenibacillus oceanisediminis KACC 16023(T), Paenibacillus barcinonensis KCTC 13019(T) were 27, 19, and 10 %, respectively. Based on the genotypic, phenotypic, and chemotaxonomic characteristics, strain DCY97(T) is considered as a novel species of the genus Paenibacillus, for which the name Paenibacillus puernese sp. nov. is proposed. The type strain is DCY97(T) (=KCTC 33596(T) = JCM 140369(T)).
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea. yeonjukim@khu.ac.kr.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Van-An
authors.initials: VA
authors.lastname: Hoang
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Ngoc-Lan
authors.initials: NL
authors.lastname: Nguyen
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea. deokchunyang@yahoo.co.kr.|Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea. deokchunyang@yahoo.co.kr.
authors.forename: Deok
authors.initials: D
authors.lastname: Chun-Yang
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005516:Food Microbiology; D005959:Glucosidases / Q000378:metabolism; D056507:Paenibacillus / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D005959:Glucosidases
keywords: Biotransformation; Fermented Pu’er tea; Paenibacillus puernese