title: COPI selectively drives maturation of the early Golgi.
abstract: COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States.
authors.forename: Effrosyni
authors.initials: E
authors.lastname: Papanikou
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States.
authors.forename: Kasey J
authors.initials: KJ
authors.lastname: Day
authors.affiliation: Electron Microscopy Core Facility, The University of Chicago, Chicago, United States.
authors.forename: Jotham
authors.initials: J
authors.lastname: Austin
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States.
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Glick
mesh_terms: D022181:COP-Coated Vesicles / Q000378:metabolism*; D020755:Coat Protein Complex I / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*
chemical_list: D020755:Coat Protein Complex I; D011506:Proteins
keywords: COPI; Golgi; Kex2; S. cerevisiae; Sec7; cell biology; cisternal maturation