title: Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment.
abstract: The synergistic effect of temperature (25-65 °C) and total specific energy input (0.55-1.11 kWh kgDW(-1)) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55 °C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55 °C. Above 55 °C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands. Electronic address: richard.postma@wur.nl.
authors.forename: P R
authors.initials: PR
authors.lastname: Postma
authors.affiliation: Department of Industrial Engineering, University of Salerno, via Giovanni Paolo II 132, Fisciano, SA, Italy. Electronic address: gpataro@unisa.it.
authors.forename: G
authors.initials: G
authors.lastname: Pataro
authors.affiliation: ProdAl Scarl - University of Salerno, via Ponte don Melillo, 84084 Fisciano, SA, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Capitoli
authors.affiliation: Food & Biobased Reseach, AlgaePARC, Wageningen University and Research Centre, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Barbosa
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands; University of Nordland, Faculty of Biosciences and Aquaculture, N-8049 Bodø, Norway.
authors.forename: R H
authors.initials: RH
authors.lastname: Wijffels
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: M H M
authors.initials: MH
authors.lastname: Eppink
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: Olivieri
authors.affiliation: Department of Industrial Engineering, University of Salerno, via Giovanni Paolo II 132, Fisciano, SA, Italy; ProdAl Scarl - University of Salerno, via Ponte don Melillo, 84084 Fisciano, SA, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ferrari
mesh_terms: D020418:Algal Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane; D005591:Chemical Fractionation / Q000379:methods*; D048408:Chlorella vulgaris / Q000737:chemistry* / Q000201:enzymology; D004560:Electricity; D055664:Electrochemical Techniques; D058086:Microalgae / Q000737:chemistry* / Q000201:enzymology; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013696:Temperature
chemical_list: D020418:Algal Proteins; D002241:Carbohydrates; D012273:Ribulose-Bisphosphate Carboxylase
keywords: Bead mill; Microalgae; Mild cascade biorefinery; Pulsed electric field; Temperature