title: Visualisation of newly synthesised collagen in vitro and in vivo.
abstract: Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize newly deposited collagen based on its inherent association with dermatan sulfate. The method is applicable irrespective of host species and collagen source.
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Biochemistry, Nijmegen, The Netherlands.
authors.forename: Corien
authors.initials: C
authors.lastname: Oostendorp
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Biochemistry, Nijmegen, The Netherlands.
authors.forename: Peter J E
authors.initials: PJ
authors.lastname: Uijtdewilligen
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Biochemistry, Nijmegen, The Netherlands.
authors.forename: Elly M
authors.initials: EM
authors.lastname: Versteeg
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Biochemistry, Nijmegen, The Netherlands.
authors.forename: Theo G
authors.initials: TG
authors.lastname: Hafmans
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Dermatology, The Netherlands.
authors.forename: Ellen H
authors.initials: EH
authors.lastname: van den Bogaard
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Urology, Nijmegen, The Netherlands.
authors.forename: Paul K J D
authors.initials: PK
authors.lastname: de Jonge
authors.forename: Ali
authors.initials: A
authors.lastname: Pirayesh
authors.affiliation: Radboud university medical center, Department of Orthodontics and Craniofacial Biology, Nijmegen, The Netherlands.
authors.forename: Johannes W
authors.initials: JW
authors.lastname: Von den Hoff
authors.affiliation: University Children's Hospital Zurich, Department of Surgery, Tissue Biology Research Unit, Zurich, Switzerland.
authors.forename: Ernst
authors.initials: E
authors.lastname: Reichmann
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Biochemistry, Nijmegen, The Netherlands.
authors.forename: Willeke F
authors.initials: WF
authors.lastname: Daamen
authors.affiliation: Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Biochemistry, Nijmegen, The Netherlands.
authors.forename: Toin H
authors.initials: TH
authors.lastname: van Kuppevelt
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003871:Dermatan Sulfate / Q000096:biosynthesis; D004285:Dogs; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D020007:Implants, Experimental; D051379:Mice; D051381:Rats; D034421:Sus scrofa; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D003871:Dermatan Sulfate; D003094:Collagen