title: Adult mouse cortical cell taxonomy revealed by single cell transcriptomics.
abstract: Nervous systems are composed of various cell types, but the extent of cell type diversity is poorly understood. We constructed a cellular taxonomy of one cortical region, primary visual cortex, in adult mice on the basis of single-cell RNA sequencing. We identified 49 transcriptomic cell types, including 23 GABAergic, 19 glutamatergic and 7 non-neuronal types. We also analyzed cell type-specific mRNA processing and characterized genetic access to these transcriptomic types by many transgenic Cre lines. Finally, we found that some of our transcriptomic cell types displayed specific and differential electrophysiological and axon projection properties, thereby confirming that the single-cell transcriptomic signatures can be associated with specific cellular properties.
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Bosiljka
authors.initials: B
authors.lastname: Tasic
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Vilas
authors.initials: V
authors.lastname: Menon
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Thuc Nghi
authors.identifier: http://orcid.org/0000-0002-6466-5883
authors.initials: TN
authors.lastname: Nguyen
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Tae Kyung
authors.initials: TK
authors.lastname: Kim
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Tim
authors.initials: T
authors.lastname: Jarsky
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Zizhen
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Boaz
authors.initials: B
authors.lastname: Levi
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Lucas T
authors.initials: LT
authors.lastname: Gray
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Staci A
authors.initials: SA
authors.lastname: Sorensen
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Tim
authors.initials: T
authors.lastname: Dolbeare
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Darren
authors.initials: D
authors.lastname: Bertagnolli
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Goldy
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Nadiya
authors.initials: N
authors.lastname: Shapovalova
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Sheana
authors.initials: S
authors.lastname: Parry
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Changkyu
authors.initials: C
authors.lastname: Lee
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Smith
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Bernard
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Madisen
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Sunkin
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Hawrylycz
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Christof
authors.initials: C
authors.lastname: Koch
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology*; D002965:Classification; D015723:Gene Library; D005819:Genetic Markers; D018698:Glutamic Acid / Q000502:physiology; D007395:Interneurons; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000145:classification; D012313:RNA / Q000235:genetics; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D014793:Visual Cortex / Q000166:cytology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D005819:Genetic Markers; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D012313:RNA