title: [Express evaluation of antioxidant activity of uracil derivatives].
abstract: Using photometric methods the antioxidant activity of 19 uracil derivatives has been analyzed. The test using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals can be applied for the rapid assessment of antioxidant activity of uracils. Among uracil derivatives studied the compounds possesing a proton-donor group in C-5 position--free or alkylated amino group, as well as hydroxyl group were the most active: 5-aminouracil (IC50 3 mg/ml), 5-amino-6-methyluracil (IC50 of 5 mg/ml), 5-hydroxy-6-methyluracil (IC50 of 15 mg/ml), 5-hydroxy-1,3,6-trimethyluracil (IC50 of 15 mg/ml), 5-ethylamino-6-methyluracil (IC50 of 20 mg/ml), 5-methylamino-6-methyluracil (IC50 of 20 mg/ml), 5-allylaminouracil (IC50 of 20 mg/ml), 5-amino-1,3,6- trimethyluracil (IC50 of 25 mg/ml). These uracil derivatives were more active than the reference compounds ionol (IC50 of 30 mg/ml) and a-naphthylamine (IC50 of 45 mg/ml), but less active than ascorbic acid (IC50 0.8 mg/ml). There was a correlation between the results of DPPH test (IC50) and coupling constants of uracil derivatives with peroxide radicals of 1,4-dioxane (fk7). Uracil with proton-donor group at C-5 also showed high ferrum-reducing activity as determined by FRAP.
authors.affiliation: Ufa Institute of Chemistry of the Russian Academy of Sciences, Ufa, Russia.
authors.forename: A R
authors.initials: AR
authors.lastname: Gimadieva
authors.affiliation: Bashkir State Agrarian University, Ufa, Russia.
authors.forename: Yu Z
authors.initials: YZ
authors.lastname: Khazimullina
authors.affiliation: Ufa Institute of Chemistry of the Russian Academy of Sciences, Ufa, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Belaya
authors.affiliation: Bashkir State University, Ufa, Russia.
authors.forename: Yu S
authors.initials: YS
authors.lastname: Zimin
authors.affiliation: Ufa Institute of Chemistry of the Russian Academy of Sciences, Ufa, Russia; Bashkir State Agrarian University, Ufa, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Abdrakhmanov
authors.affiliation: Ufa Institute of Chemistry of the Russian Academy of Sciences, Ufa, Russia; Bashkir State University, Ufa, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Mustafin
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D010783:Photometry; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D000975:Antioxidants; D014498:Uracil
keywords: 2,2-diphenyl-1-picrylhydrazyl; antioxidant activity; ferrumrestoring capacity; uracil derivatives