title: Expression of matrix metalloproteinases and ovarian morphological changes in androgenized cyclic female guinea pigs.
abstract: This study was conducted to investigate expression of matrix metalloproteinases (MMPs) and ovarian morphological changes in androgenized cyclic female guinea pigs. Adult cyclic female guinea pigs were injected daily for 28 days with medium doses of testosterone propionate (TP; 1 mg/100g), high doses of TP (2 mg/100g), or saline (control). Serum concentrations of testosterone, estradiol (E2), and progesterone (P4) were measured. Histologic sections of ovaries were stained with hematoxylin-eosin and by immunohistochemistry. Expressions of steroidogenic acute regulatory protein, proliferating cell nuclear antigen, and MMP-2 and MMP-9 in the ovary were characterized by immunohistochemistry. After 28 days of TP injection, serum testosterone concentrations were increased dose-dependently. An appropriate dosage of TP could induce permanent anovulation in guinea pigs, making them a potential model for human polycystic ovary syndrome. MMP-2 and MMP-9 are jointly involved in the growth and atresia of ovarian follicles in cyclic guinea pigs. Increased numbers of atretic antral follicles in the ovary might be associated with the observed high expression of MMP-2 in androgenized cyclic guinea pigs.
authors.affiliation: College of Agriculture and Bioengineering, Jinhua Polytechnic, Jinhua 321017, China; Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: lijornsky@163.com.
authors.forename: Jun-rong
authors.initials: JR
authors.lastname: Li
authors.affiliation: College of Agriculture and Bioengineering, Jinhua Polytechnic, Jinhua 321017, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Shen
authors.affiliation: College of Agriculture and Bioengineering, Jinhua Polytechnic, Jinhua 321017, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Quan-wei
authors.initials: QW
authors.lastname: Wei
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fang-xiong
authors.initials: FX
authors.lastname: Shi
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006168:Guinea Pigs; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D011374:Progesterone / Q000097:blood; D013739:Testosterone / Q000097:blood; D043343:Testosterone Propionate / Q000008:administration & dosage
chemical_list: D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D043343:Testosterone Propionate
keywords: Guinea pigs; Matrix metalloproteinase-2 (MMP-2); Matrix metalloproteinase-9 (MMP-9); Proliferating cell nuclear antigen (PCNA); Steroidogenic acute regulatory protein (StAR); Testosterone propionate (TP)