title: Nano-microcapsule basic fibroblast growth factor combined with hypoxia-inducible factor-1 improves random skin flap survival in rats.
abstract: The present study aimed to investigate the effect of nano-microcapsule-basic fibroblast growth factor (bFGF) combined with hypoxia-inducible factor-1 (HIF-1) on the random skin flap survival of rats. Male Sprague-Dawley rats were used to establish the McFarlane flap model and subsequently, all model rats were randomly divided into four groups: Control, bFGF, HIF-1 and bFGF combined with HIF-1. The model rats were treated with 2.5 µg/day bFGF and 1.0 µg/day HIF-1 for 5 days by intraperitoneal injection. On day 5 following treatment, the boundaries between necrotic and surviving regions were significantly inhibited by bFGF combined with HIF-1. bFGF combined with HIF-1 inhibited oxidative stresses and inflammatory factors in random skin flap survival of rats. bFGF combined with HIF-1 also activated the protein expression levels of cyclooxygenase (COX)-2 and vascular endothelial growth factor (VEGF) in the random skin flap survival of rats. In conclusion, nano-microcapsule bFGF combined with HIF-1 prevented random skin flap survival in rats through antioxidative, anti-inflammatory and activation of the protein expression levels of COX-2 and VEGF.
authors.affiliation: Department of Hand and Plastic Surgery, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Chen
authors.affiliation: Department of Hand and Plastic Surgery, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors.forename: Yi-Heng
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Hand and Plastic Surgery, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors.forename: Xia-Qing
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: Department of Hand and Plastic Surgery, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002214:Capsules / Q000008:administration & dosage*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D005786:Gene Expression Regulation; D006085:Graft Survival; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D012868:Skin Abnormalities / Q000188:drug therapy* / Q000473:pathology; D013524:Surgical Flaps / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D002214:Capsules; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D016222:Fibroblast Growth Factor 2; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat