title: Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism.
abstract: Improving our understanding of brain function requires novel tools to observe multiple physiological parameters with high resolution in vivo. We have developed a multimodal imaging system for investigating multiple facets of cerebral blood flow and metabolism in small animals. The system was custom designed and features multiple optical imaging capabilities, including 2-photon and confocal lifetime microscopy, optical coherence tomography, laser speckle imaging, and optical intrinsic signal imaging. Here, we provide details of the system's design and present in vivo observations of multiple metrics of cerebral oxygen delivery and energy metabolism, including oxygen partial pressure, microvascular blood flow, and NADH autofluorescence.
authors.affiliation: Department of Radiology, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Yaseen
authors.affiliation: Department of Radiology, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA ; Current Affiliation: Department of Biomedical Engineering, University of California, Davis, Davis, California, USA.
authors.forename: Vivek J
authors.initials: VJ
authors.lastname: Srinivasan
authors.affiliation: Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA ; Current Affiliation: Department of Biophysics and Medical Physics, Institute of Physics, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Gorczynska
authors.affiliation: Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Fujimoto
authors.affiliation: Department of Radiology, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Boas
authors.affiliation: Department of Radiology, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
authors.forename: Sava
authors.initials: S
authors.lastname: Sakadžić
keywords: (110.4190) Multiple imaging; (170.0110) Imaging systems; (170.4500) Optical coherence tomography; (180.4315) Nonlinear microscopy