title: Hyperstabilization of Tetrameric Bacillus sp. TB-90 Urate Oxidase by Introducing Disulfide Bonds through Structural Plasticity.
abstract: Bacillus sp. TB-90 urate oxidase (BTUO) is one of the most thermostable homotetrameric enzymes. We previously reported [Hibi, T., et al. (2014) Biochemistry 53, 3879-3888] that specific binding of a sulfate anion induced thermostabilization of the enzyme, because the bound sulfate formed a salt bridge with two Arg298 residues, which stabilized the packing between two β-barrel dimers. To extensively characterize the sulfate-binding site, Arg298 was substituted with cysteine by site-directed mutagenesis. This substitution markedly increased the protein melting temperature by ∼ 20 °C compared with that of the wild-type enzyme, which was canceled by reduction with dithiothreitol. Calorimetric analysis of the thermal denaturation suggested that the hyperstabilization resulted from suppression of the dissociation of the tetramer into the two homodimers. The crystal structure of R298C at 2.05 Å resolution revealed distinct disulfide bond formation between the symmetrically related subunits via Cys298, although the Cβ distance between Arg298 residues of the wild-type enzyme (5.4 Å apart) was too large to predict stable formation of an engineered disulfide cross-link. Disulfide bonding was associated with local disordering of interface loop II (residues 277-300), which suggested that the structural plasticity of the loop allowed hyperstabilization by disulfide formation. Another conformational change in the C-terminal region led to intersubunit hydrogen bonding between Arg7 and Asp312, which probably promoted mutant thermostability. Knowledge of the disulfide linkage of flexible loops at the subunit interface will help in the development of new strategies for enhancing the thermostabilization of multimeric proteins.
authors.affiliation: Department of Bioscience, Fukui Prefectural University , Fukui 910-1195, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hibi
authors.affiliation: Department of Bioscience, Fukui Prefectural University , Fukui 910-1195, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Kume
authors.affiliation: Department of Bioscience, Fukui Prefectural University , Fukui 910-1195, Japan.
authors.forename: Akie
authors.initials: A
authors.lastname: Kawamura
authors.affiliation: Department of Bioscience, Fukui Prefectural University , Fukui 910-1195, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University , Sakai, Osaka 599-8531, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Fukada
authors.affiliation: Tsuruga Institute for Biotechnology, Toyobo Company Ltd. , Tsuruga, Fukui 914-0047, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nishiya
mesh_terms: D019943:Amino Acid Substitution; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D004220:Disulfides / Q000737:chemistry*; D004795:Enzyme Stability; D020125:Mutation, Missense; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D014503:Urate Oxidase / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004220:Disulfides; D014503:Urate Oxidase