title: Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy.
abstract: In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.
authors.affiliation: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Thérapeutique, 67000 Strasbourg, France. a.bianco@ibmc-cnrs.unistra.fr.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Spinato
authors.forename: Aritz
authors.initials: A
authors.lastname: Perez Ruiz de Garibay
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kierkowicz
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pach
authors.forename: Markus
authors.initials: M
authors.lastname: Martincic
authors.forename: Rebecca
authors.initials: R
authors.lastname: Klippstein
authors.forename: Maxime
authors.initials: M
authors.lastname: Bourgognon
authors.forename: Julie Tzu-Wen
authors.initials: JT
authors.lastname: Wang
authors.forename: Cécilia
authors.initials: C
authors.lastname: Ménard-Moyon
authors.forename: Khuloud T
authors.initials: KT
authors.lastname: Al-Jamal
authors.forename: Belén
authors.initials: B
authors.lastname: Ballesteros
authors.forename: Gerard
authors.initials: G
authors.lastname: Tobias
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
mesh_terms: D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D000068818:Cetuximab / Q000008:administration & dosage*; D003412:Cricetulus; D066246:ErbB Receptors; D006801:Humans; D008670:Metals / Q000008:administration & dosage*; D037742:Nanotubes, Carbon; D009369:Neoplasms / Q000532:radiotherapy*; D011878:Radiotherapy
chemical_list: D008670:Metals; D037742:Nanotubes, Carbon; D066246:ErbB Receptors; D000068818:Cetuximab