title: Identification and Characterization of FabA from the Type II Fatty Acid Synthase of Streptomyces coelicolor.
abstract: FabA is proposed to catalyze the dehydration step of chain elongation in fatty acid and undecylprodiginine biosynthesis in Streptomyces coelicolor. Analysis of the S. coelicolor genome has revealed a fabA gene (SCO4636-SCO4637, encoding a heterodimer 3-hydroxyacyl-ACP dehydratase). Herein, we report the identification and characterization of the corresponding gene products. Kinetic analysis has demonstrated that FabA is capable of utilizing various chain lengths of straight- and branched-chain 3-hydroxyacyl-NAC substrates. Additionally, FabA does not discriminate between acyl carrier proteins (ACPs) from primary and secondary metabolism. These data provide the first experimental evidence that FabA has 3-hydroxyacyl-ACP dehydratase activity and processes intermediates for both biosynthetic pathways.
authors.affiliation: Department of Chemistry, Portland State University , Portland, Oregon 97201, United States.
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Portland State University , Portland, Oregon 97201, United States.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Reynolds
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism; D002384:Catalysis; D004746:Enoyl-CoA Hydratase / Q000378:metabolism*; D054889:Fatty Acid Synthase, Type II / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015394:Molecular Structure; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D000213:Acyl Carrier Protein; D005227:Fatty Acids; C528938:undecylprodiginine; C000608320:3-hydroxyacyl-ACP dehydratase, Streptomyces coelicolor; D004746:Enoyl-CoA Hydratase; D054889:Fatty Acid Synthase, Type II; D011353:Prodigiosin