title: [TEM and CTX-M extended-spectrum beta-lactamase in Klebsiella spp and Escherichia coli isolates from inanimate surfaces of hospital environments].
abstract: The aim of the study was to determine the genotype of 15 ESBL strains of Enterobacteriaceae resistant to beta-lactams, isolated from inanimate surfaces and phenotypically characterized as producing extended-spectrum beta-lactamase. After evaluation and screening of the bacterial strains, a PCR was conducted to amplify fragments of 1078 bp and 544 bp corresponding to type TEM and CTX-M ESBL. Eleven strains presented both fragments at the time and only three had blaCTX-M. In conclusion, the presence of ESBL genes in cultures from the environment was demonstrated, some of which may belong to more than one type. This information could serve as a basis for implementing preventive measures to prevent the transmission of multiresistant bacteria from inanimate surfaces to patients, mainly in critical hospital areas.
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Nacional de Cajamarca, Cajamarca, Perú
authors.forename: Marco
authors.initials: M
authors.lastname: Rivera-Jacinto
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Nacional de Cajamarca, Cajamarca, Perú
authors.forename: Claudia
authors.initials: C
authors.lastname: Rodríguez-Ulloa
authors.affiliation: Hospital Regional de Lambayeque, Lambayeque, Perú
authors.forename: René
authors.initials: R
authors.lastname: Flores Clavo
authors.affiliation: Hospital Regional de Lambayeque, Lambayeque, Perú
authors.forename: Luis
authors.initials: L
authors.lastname: Serquén López
authors.affiliation: Universidad Católica Santo Toribio de Mogrovejo, Lambayeque, Perú
authors.forename: Zhandra
authors.initials: Z
authors.lastname: Arce Gil
mesh_terms: D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000188:drug therapy; D006761:Hospitals; D006801:Humans; D007709:Klebsiella / Q000201:enzymology*; D007710:Klebsiella Infections / Q000188:drug therapy; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases; D047090:beta-Lactams
chemical_list: D047090:beta-Lactams; D001618:beta-Lactamases