title: Effect of Selenium Against Lead-Induced Damage on the Gene Expression of Heat Shock Proteins and Inflammatory Cytokines in Peripheral Blood Lymphocytes of Chickens.
abstract: The possible beneficial role of selenium (Se) in heat shock proteins (HSPs) and inflammation damage induced by lead (Pb) in chickens is unclear. Therefore, the aim of this study was to investigate the effect of Se against Pb on the messenger RNA (mRNA) expression levels of HSPs (HSP 27, 40, 60, 70, and 90); heme oxygenase-1 (HO-1); and the inflammatory cytokines nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in the peripheral blood lymphocytes of chickens. A total of 360 1-day-old broiler chickens were randomly allocated into four groups (n = 90/group). The control group was fed a basic diet containing 0.2 mg/kg Se and 0.5 mg/kg Pb; the Se supplementation group (+Se group) was fed a Se-adequate (sodium selenite) diet containing 1 mg/kg Se and 0.5 mg/kg Pb; the Pb-supplemented group (+Pb group) was fed a Pb acetate diet containing 0.2 mg/kg Se and 350 mg/kg Pb; and the Se and Pb compound group (Se + Pb group) was fed a diet containing 1 mg/kg Se and 350 mg/kg Pb. The blood was collected and examined for the mRNA levels of HSP and inflammatory cytokine genes at 30 and 60 days old. The results showed that Pb poisoning induced the mRNA expression of HSPs and inflammatory cytokines in the peripheral blood lymphocytes of chickens. In addition, Se alleviated the Pb-induced increase in HSP and inflammatory cytokine mRNA levels in chicken peripheral blood lymphocytes. In conclusion, Se can antagonize the toxic effects of Pb on chickens and protect the chickens' peripheral blood lymphocytes in normal physiological function.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: G X
authors.initials: GX
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: C P
authors.initials: CP
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. lishu@neau.edu.cn.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China. jf699@cornell.edu.|Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. jf699@cornell.edu.
authors.forename: J
authors.initials: J
authors.lastname: Fu
mesh_terms: D000818:Animals; D002645:Chickens / Q000097:blood / Q000235:genetics; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism; D007854:Lead / Q000008:administration & dosage / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D007854:Lead; D012643:Selenium
keywords: Chicken peripheral blood lymphocytes; Heat shock protein; Inflammatory cytokines; Lead; Selenium