title: Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives.
abstract: Seed dormancy in rice is an important trait related to the pre-harvest sprouting resistance. In order to understand the molecular mechanisms of seed dormancy, gene expression was investigated by transcriptome analysis using seeds of the strongly dormant cultivar N22 and its less dormant mutants Q4359 and Q4646 at 24 days after heading (DAH). Microarray data revealed more differentially expressed genes in Q4359 than in Q4646 compared to N22. Most genes differing between Q4646 and N22 also differed between Q4359 and N22. GO analysis of genes differentially expressed in both Q4359 and Q4646 revealed that some genes such as those for starch biosynthesis were repressed, whereas metabolic genes such as those for carbohydrate metabolism were enhanced in Q4359 and Q4646 seeds relative to N22. Expression of some genes involved in cell redox homeostasis and chromatin remodeling differed significantly only between Q4359 and N22. The results suggested a close correlation between cell redox homeostasis, chromatin remodeling and seed dormancy. In addition, some genes involved in ABA signaling were down-regulated, and several genes involved in GA biosynthesis and signaling were up-regulated. These observations suggest that reduced seed dormancy in Q4359 was regulated by ABA-GA antagonism. A few differentially expressed genes were located in the regions containing qSdn-1 and qSdn-5 suggesting that they could be candidate genes underlying seed dormancy. Our work provides useful leads to further determine the underling mechanisms of seed dormancy and for cloning seed dormancy genes from N22.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Baoxu
authors.initials: B
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tong
authors.affiliation: Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Langtao
authors.initials: L
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: jiangling@njau.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism; D046228:Microarray Analysis; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D057445:Plant Dormancy / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: D005875:Gibberellins; D000040:Abscisic Acid
keywords: ABA-GA; Germination; Microarray; Oryza sativa; Seed dormancy