title: Non-toxic O-quaternized chitosan materials with better water solubility and antimicrobial function.
abstract: Five water-soluble O-quaternary ammonium salt-chitosans (QAS-CS) bearing N-methyl-N-R-N, N-bis(2-hydroxyethyl) ammonium bromides (R=-benzyl (chloride, BNQAS-CS), -dodecyl (C12QAS-CS), -tetradecyl (C14QAS-CS), -hexadecyl (C16QAS-CS), -octadecyl (C18QAS-CS)) were prepared, respectively. They were characterized by FTIR, (1)H NMR and elemental analysis. Through chemical modification of O-quaternized chitosans, the water solubility of all QAS-CS was improved distinctly. Their antibacterial properties indicate good antibacterial abilities against gram-positive bacteria and bad against gram-negative bacteria, therein, C12QAS-CS and C14QAS-CS are the best. More importantly, their cytotoxicity was markedly lower than the corresponding QAS monomers by evaluating for AT2 cell line using CCK-8 assay. The strategy provides a facile way to design and develop new types of antibacterial chitosan materials with better water solubility, better antimicrobial ability and lower cytotoxicity for primary additive agent of self-owned intellectual band-aids.
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China. Electronic address: chunhuawang508@126.com.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wang
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China.
authors.forename: Wen-Shuai
authors.initials: WS
authors.lastname: Liu
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China.
authors.forename: Ju-Feng
authors.initials: JF
authors.lastname: Sun
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China. Electronic address: guigehou@163.com.
authors.forename: Gui-Ge
authors.initials: GG
authors.lastname: Hou
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.affiliation: School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D048271:Chitosan
keywords: Antibacterial; Chitosan (CS); Cytotoxicity; Quaternary ammonium salt (QAS-CS); Water solubility