title: In Vitro Analysis of Fibronectin-Modified Titanium Surfaces.
abstract: BACKGROUND
  Glow discharge plasma (GDP) procedure is an effective method for grafting various proteins, including albumin, type I collagen, and fibronectin, onto a titanium surface. However, the behavior and impact of titanium (Ti) surface modification is yet to be unraveled.
  
  
  OBJECTIVE
  The purpose of this study is to evaluate and analyze the biological properties of fibronectin-grafted Ti surfaces treated by GDP.
  
  
  METHODS
  Grade II Ti discs were initially cleaned and autoclaved to obtain original specimens. Subsequently, the specimens were GDP treated and grafted with fibronectin to form Ar-GDP (Argon GDP treatment only) and GDP-fib (fibronectin coating following GDP treatment) groups. Blood coagulation test and MG-63 cell culture were performed to evaluate the biological effects on the specimen.
  
  
  RESULTS
  There was no significant difference between Ar-GDP and GDP-fib groups in blood compatibility analysis. While in the MTT test, cellular proliferation was benefited from the presence of fibronectin coating. The numbers of cells on Ar-GDP and GDP-fib specimens were greater than those in the original specimens after 24 h of culturing.
  
  
  CONCLUSIONS
  GDP treatment combined with fibronectin grafting favored MG-63 cell adhesion, migration, and proliferation on titanium surfaces, which could be attributed to the improved surface properties.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Chang
authors.affiliation: School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Lee
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Feng
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.|Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Haw-Ming
authors.initials: HM
authors.lastname: Huang
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.|Dental Department, Taipei Medical University Hospital, Taipei, Taiwan, ROC.
authors.forename: Che-Tong
authors.initials: CT
authors.lastname: Lin
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.|Dental Department, Taipei Medical University Hospital, Taipei, Taiwan, ROC.
authors.forename: Nai-Chia
authors.initials: NC
authors.lastname: Teng
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan, ROC.|Dental Department of Taipei Medical University, Shuang-Ho Hospital, Taipei, Taiwan, ROC.
authors.forename: Wei Jen
authors.initials: WJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D005353:Fibronectins / Q000032:analysis*; D008422:Materials Testing / Q000379:methods; D010006:Osteoblasts / Q000166:cytology; D011817:Rabbits; D013499:Surface Properties; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D014025:Titanium