title: ADAM12 expression predicts clinical outcome in estrogen receptor-positive breast cancer.
abstract: OBJECTIVE
  Our study was aimed to make sure whether ADAM12 could serve as a prognostic biomarker of estrogen receptor (ER) -positive breast cancer.
  
  
  METHODS
  127 patients with ER-positive breast cancer were included in the present study. The level of ADAM12 was assayed through real-time quantitative PCR (RT-qPCR). Levels of ADAM12 in tumor tissues and adjacent normal tissues were compared with paired t-test. The association of ADAM12 expression with clinical characteristics was analyzed via χ(2) test. Kaplan-Meier survival curve was used to evaluate the role of ADAM12 expression in overall survival (OS) of patients. Cox-regression analysis was performed to judge if ADAM12 could serve as a prognostic marker in breast cancer.
  
  
  RESULTS
  The level of ADAM12 was upregulated in tumor tissues of breast cancer compared to that of adjacent normal tissues (P < 0.05). The expression of ADAM12 was closely related to the Ki-67 and HER2 status (P < 0.05 for both). The results of Kaplan-Meier survival curve showed that patients with higher level of ADAM12 exhibited shorter survival time compared to that of low level of ADAM12 (P < 0.001). Cox regression analysis showed that ADAM12 might be a biomarker in predicting prognosis of patients with ER-positive breast cancer (HR = 7.116, 95% CI = 3.329-15.212).
  
  
  CONCLUSIONS
  ADAM12 appears to be a prognostic marker in ER-positive breast cancer.
authors.affiliation: Affiliated Central Hospital of Huzhou Teachers College Huzhou, Zhejiang 313000, China ; University Hospital Tuebingen, Germany.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: University Hospital Tuebingen, Germany.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Wuxi Hospital of Traditional Chinese Medicine China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Jin
authors.affiliation: Affiliated Central Hospital of Huzhou Teachers College Huzhou, Zhejiang 313000, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Affiliated Central Hospital of Huzhou Teachers College Huzhou, Zhejiang 313000, China.
authors.forename: Guolei
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Affiliated Central Hospital of Huzhou Teachers College Huzhou, Zhejiang 313000, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Zhang
authors.affiliation: University Hospital Tuebingen, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Seeger
authors.affiliation: University Hospital Tuebingen, Germany.
authors.forename: Alfred O
authors.initials: AO
authors.lastname: Mueck
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000072199:ADAM12 Protein; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism; D015996:Survival Rate; D015854:Up-Regulation
chemical_list: D051722:ADAM Proteins; D000072199:ADAM12 Protein; D014408:Biomarkers, Tumor; D008565:Membrane Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C000606480:ADAM12 protein, human
keywords: ADAM12; breast cancer; clinical outcome; estrogen receptor