title: Expression of chemokine receptor CXCR7 in colorectal carcinoma and its prognostic significance.
abstract: Previous studies have shown that chemokine receptor CXCR7 plays critical roles in tumor development. However, the clinicopathological and prognostic significance of CXCR7 in colorectal carcinoma (CRC) has not been fully understood. The aim of our study is to investigate the expression of CXCR7 and its clinical significance in CRC. First, quantitative RT-PCR and Western blot assays were performed to determine the expression of CXCR7 mRNA and protein in 20 paired of CRC tissues and corresponding adjacent non-tumor tissues. Next, immunohistochemistry was performed to detect the expression of CXCR7 protein in another 96 cases of CRC tissues, and analyze its correlation with clinicopathological factors of patients. Finally, the correlation of CXCR7 with 5-year overall survival (OS) and progression free survival (PFS) was statistically analyzed by the Kaplan-Meier method and Cox proportional hazards model. Results showed that the expression levels of CXCR7 mRNA and protein were significantly higher in CRC tissues than in normal tissues. Positive CXCR7 expression was observed to be significantly correlated with lymph nodal metastasis (P < 0.001), distant metastasis (P = 0.017), and advanced TNM stage (P < 0.001). Patients with positive expression of CXCR7 were demonstrated to be associated with worse OS and PFS (P < 0.001, P < 0.001, respectively). Moreover, multivariate survival analysis revealed that CXCR7 expression level might be an independent predictive factor for OS and PFS of CRC patients. Collectively, positive CXCR7 expression in CRC was correlated with tumor development and poor prognosis of patients.
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Dai
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Longbang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing Clinical School of Southern Medical University Nanjing, Jiangsu Province, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D054387:Receptors, CXCR / Q000032:analysis / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C501896:ACKR3 protein, human; D014408:Biomarkers, Tumor; D054387:Receptors, CXCR
keywords: CXCR7; Colorectal carcinoma; immunohistochemistry; overall survival; progression free survival