title: Antioxidant and anti-aging activities of the polysaccharide TLH-3 from Tricholoma lobayense.
abstract: Polysaccharides from edible fungi usually exhibit many bioactivities. Our previous studies found that polysaccharide TLH-3 extracted from Tricholoma lobayense possessed noticeable antioxidant activity. To further explore its biological activities, the antioxidant and anti-aging activities of TLH-3 were evaluated in vitro and in vivo. The results of antioxidant activity in vitro showed that TLH-3 could enhance the cell viability, reduce the production of reactive oxygen species (ROS) and inhibit oxidative damage induced by tert-butylhydroperoxide (t-BHP) in human embryonic lung fibroblasts (HELF) cells. The anti-aging capability was measured in d-galactose (d-gal)-induced aged mice model, and the experimental data showed that TLH-3 significantly inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and catalase (CAT) in mice liver and serum (p<0.05). This study suggested that TLH-3 possessed apparent antioxidant and anti-aging activities and could be exploited as a potent dietary supplement to attenuate aging and prevent age-related diseases in humans.
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Ding
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Lu
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Li
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Qingxi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Pan
authors.affiliation: School of life Sciences, Anhui University, Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, Hefei 230601, Anhui, PR China. Electronic address: chenyan91030@yahoo.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005690:Galactose / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005690:Galactose
keywords: Anti-aging activity; Antioxidant activity; Tricholoma lobayense polysaccharide TLH-3