title: Nanog interact with CDK6 to regulates astrocyte cells proliferation following spinal cord injury.
abstract: Previous research had reported transcription factors Nanog expressed in pluripotent embryonic stem cells (ESCS) that played an important role in regulating the cell proliferation. Nanog levels are frequently elevated in ESCS, but the role in the spinal cord was not clear. To examine the biological relevance of Nanog, we studied its properties in spinal cord injury model. The expression of Nanog and PCNA was gradually increased and reached a peak at 3 day by western blot analysis. The expression of Nanog was further analyzed by immunohistochemistry. Double immunofluorescent staining uncovered that Nanog can co-labeled with PCNA and GFAP in the spinal cord tissue. In vitro, Nanog can promote the proliferation of astrocyte cell by Fluorescence Activating Cell Sorter (FACS) and CCK8. Meanwhile, the cell-cycle protein CDK6 could interact with Nanog in the spinal cord tissue. Taken together, these data suggested that both Nanog may play important roles in spinal cord pathophysiology via interact with CDK6.
authors.affiliation: Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu, China; Department of Orthopaedics, Xishan People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Orthopaedics, Xishan People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Orthopaedics, Xishan People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Xishan People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Xu
authors.affiliation: Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu, China. Electronic address: caizhengdongsh@163.com.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D051361:Cyclin-Dependent Kinase 6 / Q000378:metabolism*; D008297:Male; D000071317:Nanog Homeobox Protein; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000071317:Nanog Homeobox Protein; C547030:Nanog protein, rat; D014157:Transcription Factors; C495913:Cdk6 protein, rat; D051361:Cyclin-Dependent Kinase 6
keywords: Astrocyte; CDK6; Nanog; Proliferation; Spinal cord