title: Identification of Single Nucleotide Polymorphism Marker and Association Analysis of Marbling Score in Fas Gene of Hanwoo.
abstract: The Fas (APO-1, TNFRSF6) gene known as a member of the tumor necrosis factor receptor superfamily was selected for DNA marker development in Korean cattle. It is a cell membrane protein and mediates programmed cell death (apoptosis). We discovered single nucleotide polymorphisms (SNPs) within Fas gene in order to develop novel DNA markers related to economical traits at the genomic level. The sequences of whole exon and 1 kb range of both front and back of the gene were determined by direct-sequencing methods using 24 cattle. A total of 55 SNPs were discovered and we selected 31 common polymorphic sites considering their allele frequencies, haplotype-tagging status and linkage disequilibrium (LD) for genotyping in larger-scale subjects. The SNPs were confirmed genotype through the SNaPshot method (n = 274) and were examined for a possible genetic association between Fas polymorphisms and marbling score. So, the SNPs that were identified significant are g.30256G>C, g.31474C>A, g.31940A>G, and g.32982G>A. These results suggest that SNPs of Fas gene were associated with intramuscular fat content of meat quality traits in Korean cattle.
authors.affiliation: Devision of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea .
authors.forename: Seung-Chang
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Devision of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea .
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Devision of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea .
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Devision of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea .
authors.forename: Tae-Hun
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Devision of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, Korea .
authors.forename: Bong-Hwan
authors.initials: BH
authors.lastname: Choi
keywords: Fas; Hanwoo; Linkage Disequilibrium; Marbling Score; Single Nucleotide Polymorphism