title: Effects of induced pluripotent stem cells-derived conditioned medium on the proliferation and anti-apoptosis of human adipose-derived stem cells.
abstract: Human adipose-derived stem cells (hASCs) become an appealing source for regenerative medicine. However, with the multi-passage or cryopreservation for large-scale growth procedures in terms of preclinical and clinical purposes, hASCs often reveal defective cell viability, which is a major obstacle for cell therapy. In our study, the effects of induced pluripotent stem cells-derived conditioned medium (iPS-CM) on the proliferation and anti-apoptosis in hASCs were investigated. hASCs at passage 1 were identified by the analysis of typical surface antigens with flow cytometry assay and adipogenic and osteogenic differentiation. The effect of iPS-CM on the proliferation in hASCs was analyzed by cell cycle assay and Ki67/P27 quantitative polymerase chain reaction analysis. The effect of iPS-CM on the anti-apoptosis of hASCs irradiated by 468 J/m(2) of ultraviolet C was investigated by annexin v/propidium iodide analysis, mitochondrial membrane potential assay, intracellular reactive oxygen species assay, Western blotting and caspase activity assays. The effect of iPS-CM on the surface antigen expressions of hASCs was analyzed using flow cytometry assay. The levels of Activin A and bFGF in culture supernatant of hASCs with different treatments were also detected by enzyme-linked immunosorbent assay. iPS-CM promoted proliferation and inhibited apoptosis of hASCs. This discovery demonstrates that iPS-CM might be used as one of the available means to overcome the propagation obstacle for hASCs and make for large-scale growth procedures in terms of preclinical and clinical purposes.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Jinan University, Guangzhou, 510632, Guangdong, China.
authors.forename: Rui-Ling
authors.initials: RL
authors.lastname: Lian
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, 510632, Guangdong, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Jinan University, Guangzhou, 510632, Guangdong, China. chenjiansu2000@163.com.|Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, 510632, Guangdong, China. chenjiansu2000@163.com.|Institute of Ophthalmology, Medical College of Jinan University, #601 West Huangpu Avenue, Guangzhou, 510632, Guangdong, China. chenjiansu2000@163.com.
authors.forename: Jian-Su
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, 510632, Guangdong, China.
authors.forename: Yong-Long
authors.initials: YL
authors.lastname: Guo
authors.affiliation: Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, Guangdong, China.
authors.forename: Jia-Fu
authors.initials: JF
authors.lastname: Zheng
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Jinan University, Guangzhou, 510632, Guangdong, China.
authors.forename: Yuan-Wen
authors.initials: YW
authors.lastname: Chen
mesh_terms: D028341:Activins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000954:Antigens, Surface / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D017077:Culture Media, Conditioned; C422405:activin A; D016222:Fibroblast Growth Factor 2; D028341:Activins
keywords: Activin A; Apoptosis; Proliferation; bFGF; hASCs; iPS-CM