title: Preparation of lactoperoxidase incorporated hybrid nanoflower and its excellent activity and stability.
abstract: We report a green approach to synthesize lactoperoxidase (LPO) enzyme and metal ions hybrid nanoflowers (HNFs) and investigate mechanism underlying formation and enhanced catalytic activity and stability under different experimental parameters. The HNFs formed of LPO enzyme purified from bovine milk and copper ions (Cu(2+)) were synthesized at two different temperatures (+4 °C and 20 °C) in PBS (pH 7.4). The effects of experimental conditions, pH and storage temperatures, on the activity and stability of LPO-copper phosphate HNFs were evaluated using guaiacol as a substrate in the presence of hydrogen peroxide (H2O2). Optimum pHs were determined as pH 8 and pH 6 for LPO-copper phosphate HNF and free LPO, respectively. LPO-copper phosphate HNF has higher activity than free LPO at each pHs. Activities of LPO-copper phosphate HNF at pH 6 and pH 8 were calculated as 70.48 EU/mg, 107.23 EU/mg, respectively while free LPO shows 45.78 EU/mg and 30.12 EU/mg, respectively. Compared with free LPO, LPO-copper phosphate HNFs exhibited ∼160% and ∼360% increase in activities at pH 6 and pH 8, respectively. Additionally, LPO-copper phosphate HNFs displayed perfect reusability after six cycles. Finally, we demonstrated that LPO-copper phosphate HNFs can be utilized as a nanosensor for detection of dopamine and epinephrine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey; Nanotechnology Research Center, Erciyes University, Kayseri 38039, Turkey.
authors.forename: Cevahir
authors.initials: C
authors.lastname: Altinkaynak
authors.affiliation: Department of Chemistry, Faculty of Science, Erciyes University, Kayseri 38039, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Yilmaz
authors.affiliation: Department of Chemistry, Faculty of Science, Ataturk University, Erzurum 25030, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Koksal
authors.affiliation: Department of Chemistry, Faculty of Science, Ataturk University, Erzurum 25030, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Özdemir
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey; Nanotechnology Research Center, Erciyes University, Kayseri 38039, Turkey. Electronic address: ismailocsoy66@gmail.com.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ocsoy
authors.affiliation: Department of Chemistry, Faculty of Science, Erciyes University, Kayseri 38039, Turkey.
authors.forename: Nalan
authors.initials: N
authors.lastname: Özdemir
mesh_terms: D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry; D004298:Dopamine / Q000378:metabolism; D004789:Enzyme Activation; D004795:Enzyme Stability; D004837:Epinephrine / Q000378:metabolism; D007700:Kinetics; D007784:Lactoperoxidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D008892:Milk / Q000737:chemistry / Q000201:enzymology; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010710:Phosphates; D003300:Copper; D007784:Lactoperoxidase; D004298:Dopamine; D004837:Epinephrine
keywords: Copper ions; Dopamine; Enzymatic activity; Epinephrine; Hybrid nanoflower; Lactoperoxidase (LPO)