title: [Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration].
abstract: Bone tissue damages are one of the dominant causes of temporary disability and developmental disability. Currently, there are some methods of guided bone regeneration employing different osteoplastic materials and insulation membranes used in surgery. In this study, we have developed a method of preparation of porous membranes from the biopolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), produced by a strain of Azotobacter chroococcum 7B. The biocompatibility of the porous membranes was investigated in vitro using mesenchymal stem cells (MSCs) and in vivo on laboratory animals. The cytotoxicity test showed the possibility of cell attachment on membrane and histological studies confirmed good insulating properties the material. The data obtained demonstrate the high biocompatibility and the potential application of insulating membranes based on PHBV in bone tissue engineering.
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: S Yu
authors.initials: SY
authors.lastname: Ivanov
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia; Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Bonartsev
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Gazhva
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Zharkova
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: R F
authors.initials: RF
authors.lastname: Mukhametshin
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: T K
authors.initials: TK
authors.lastname: Mahina
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V L
authors.initials: VL
authors.lastname: Myshkina
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Bonartseva
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Voinova
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Andreeva
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Akulina
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Kharitonova
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Shaitan
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Muraev
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D005260:Female; D050723:Fractures, Bone / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D008297:Male; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011817:Rabbits; D013552:Swine; D013556:Swine, Miniature; D000094122:Polyhydroxybutyrates
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: bone tissue; guided bone regeneration; isolating membrane; poly(3-hydroxybutyrate-co-3-hydroxyvalerate); porous scaffold