title: Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection.
abstract: Intraoperative 5- aminolevulinic acid induced-Protoporphyrin IX (PpIX) fluorescence guidance enables maximum safe resection of glioblastomas by providing surgeons with real-time tumor optical contrast. However, visual assessment of PpIX fluorescence is subjective and limited by the distorting effects of light attenuation and tissue autofluorescence. We have previously shown that non-invasive point measurements of absolute PpIX concentration identifies residual tumor that is otherwise non-detectable. Here, we extend this approach to wide-field quantitative fluorescence imaging by implementing spatial frequency domain imaging to recover tissue optical properties across the field-of-view in phantoms and ex vivo tissue.
authors.affiliation: Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON M5G 1L7 Canada ; Princess Margaret Cancer Center/University Health Network, 610 University Avenue, Toronto, ON M5G 2M3 Canada.
authors.forename: Mira
authors.initials: M
authors.lastname: Sibai
authors.affiliation: Princess Margaret Cancer Center/University Health Network, 610 University Avenue, Toronto, ON M5G 2M3 Canada.
authors.forename: Israel
authors.initials: I
authors.lastname: Veilleux
authors.affiliation: Thayer School of Engineering at Dartmouth, Thayer School of Engineering at Dartmouth 14 Engineering Drive Hanover, NH USA 03755 USA.
authors.forename: Jonathan T
authors.initials: JT
authors.lastname: Elliott
authors.affiliation: École Polytechnique De Montreal, 2900, boul. Édouard-Montpetit Montréal, Québec H3T 1J4 Canada.
authors.forename: Frederic
authors.initials: F
authors.lastname: Leblond
authors.affiliation: Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON M5G 1L7 Canada ; Princess Margaret Cancer Center/University Health Network, 610 University Avenue, Toronto, ON M5G 2M3 Canada.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
keywords: (110.0110) Imaging systems; (110.0113) Imaging through turbid media; (170.6510) Spectroscopy, tissue diagnostics