title: Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward.
abstract: Gene editing in zebrafish has begun to reveal discordance between mutant phenotypes and those associated with knockdown via morpholino oligonucleotides (MOs). These studies suggest that MOs should not be used as a standalone tool and underscore the need for guidelines that require defined mutants to assess gene function in zebrafish.
authors.affiliation: Department of Molecular, Cell, and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address: nathan.lawson@umassmed.edu.
authors.forename: Nathan D
authors.initials: ND
authors.lastname: Lawson
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D055785:Gene Knockdown Techniques / Q000379:methods / Q000639:trends; D006801:Humans; D060172:Morpholinos / Q000235:genetics; D016296:Mutagenesis / Q000502:physiology; D009154:Mutation / Q000235:genetics; D059386:Reverse Genetics / Q000379:methods / Q000639:trends; D015027:Zebrafish / Q000235:genetics*
chemical_list: D060172:Morpholinos