title: [Glutathione redox system, immune status, antioxidant enzymes and metabolism of purine nucleotides in hypothyroidism].
abstract: The immune status, components of the glutathione redox system, the activity of antioxidant enzymes and metabolism of purine nucleotides have been investigated in animals with experimental hypothyroidism. On day 8 after an increase in the number of leukocytes, lymphocytes, T-helpers and T-suppressors as well as increased number of B-lymphocytes was found in blood of thyroidectomized rats. This was accompanied by decreased activity of adenosine deaminase (AD), AMP-deaminase (AMPD), and 5'-nucleotidase (5'N) in blood, but the ratio of enzyme activity AD/AMPD increased. These changes in the activity of enzymes, involved in purine catabolism can be regarded as increased functional relationships between T and B lymphocytes in hypothyroidism. The functional changes of immune system cells were accompanied by increased activity of glutathione peroxidase (GPx), a decrease in the activity of superoxide dismutase (SOD), glutathione reductase (GR) and the ratio GH/GPx. Thyroidectomized rats had increased amounts of total, oxidized (GSSG) and reduced glutathione (GSH), but the ratio GSH/GSSG decerased as compared with control animals. In the liver, hypothyroidism resulted in activation of SOD, GPx, decreased activity of GR and decreased ratio GR/GPx. At the same time, the levels of total, oxidized, and reduced glutathione increased, but the ratio GSH/GSSG as well as activities of enzymes involved in purine nucleotide metabolism ratio (and their ratio 5'N/AD + AMPD) decreased. All these data suggest a functional relationship of the glutathione redox system not only with antioxidant enzymes, but also activity of enzymes involved purine nucleotide metabolism and immune status.
authors.affiliation: Semey State Medical University, Semey, Kazakhstan.
authors.forename: S O
authors.initials: SO
authors.lastname: Tapbergenov
authors.affiliation: Semey State Medical University, Semey, Kazakhstan.
authors.forename: B S
authors.initials: BS
authors.lastname: Sovetov
authors.affiliation: Semey State Medical University, Semey, Kazakhstan.
authors.forename: R B
authors.initials: RB
authors.lastname: Bekbosynova
authors.affiliation: Semey State Medical University, Semey, Kazakhstan.
authors.forename: S M
authors.initials: SM
authors.lastname: Bolysbekova
mesh_terms: D000818:Animals; D000975:Antioxidants; D005978:Glutathione / Q000276:immunology*; D005979:Glutathione Peroxidase / Q000276:immunology*; D007037:Hypothyroidism / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D011685:Purine Nucleotides / Q000276:immunology*; D051381:Rats
chemical_list: D000975:Antioxidants; D011685:Purine Nucleotides; D005979:Glutathione Peroxidase; D005978:Glutathione
keywords: enzymes of purine metabolism; glutathione; glutathione reductase; hypothyroidism