title: Conserved Curvature of RNA Polymerase I Core Promoter Beyond rRNA Genes: The Case of the Tritryps.
abstract: In trypanosomatids, the RNA polymerase I (RNAPI)-dependent promoters controlling the ribosomal RNA (rRNA) genes have been well identified. Although the RNAPI transcription machinery recognizes the DNA conformation instead of the DNA sequence of promoters, no conformational study has been reported for these promoters. Here we present the in silico analysis of the intrinsic DNA curvature of the rRNA gene core promoters in Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major. We found that, in spite of the absence of sequence conservation, these promoters hold conformational properties similar to other eukaryotic rRNA promoters. Our results also indicated that the intrinsic DNA curvature pattern is conserved within the Leishmania genus and also among strains of T. cruzi and T. brucei. Furthermore, we analyzed the impact of point mutations on the intrinsic curvature and their impact on the promoter activity. Furthermore, we found that the core promoters of protein-coding genes transcribed by RNAPI in T. brucei show the same conserved conformational characteristics. Overall, our results indicate that DNA intrinsic curvature of the rRNA gene core promoters is conserved in these ancient eukaryotes and such conserved curvature might be a requirement of RNAPI machinery for transcription of not only rRNA genes but also protein-coding genes.
authors.affiliation: Molecular Interactions Laboratory, School of Sciences, University of the Republic, Montevideo 11400, Uruguay; Genetics Departament, School of Medicine, University of the Republic, Montevideo 11800, Uruguay.
authors.forename: Pablo
authors.initials: P
authors.lastname: Smircich
authors.affiliation: Molecular Interactions Laboratory, School of Sciences, University of the Republic, Montevideo 11400, Uruguay; Genetics Departament, School of Medicine, University of the Republic, Montevideo 11800, Uruguay.
authors.forename: María Ana
authors.initials: MA
authors.lastname: Duhagon
authors.affiliation: Molecular Interactions Laboratory, School of Sciences, University of the Republic, Montevideo 11400, Uruguay. Electronic address: bgarat@fcien.edu.uy.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Garat
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D020459:Genes, rRNA; D018320:Leishmania major / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000378:metabolism*; D016053:RNA, Protozoan / Q000737:chemistry* / Q000235:genetics; D012335:RNA, Ribosomal; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D012318:RNA Polymerase I
keywords: Core promoter; Intrinsic curvature; Leishmania; RNA polymerase I; Trypanosoma