title: Application of different enzyme assays and biomarkers for pollution monitoring of the marine environment.
abstract: New phosphatase and DNase inhibition tests for assessing the total pollution of a natural marine ecosystem were applied. The seawater samples with different pollution degrees were collected in the Troitsa Bay of the Peter the Great Bay (the Sea of Japan). The sensitivity of the alkaline phosphatase test to integrated pollution was in accordance with the sensitivity of the standard sea urchin sperm cell toxicity test. The increased seawater pollution level was shown to result in an up to fourfold increase in specific activities of acid and alkaline phosphatases from the mussel Crenomytilus grayanus. It was demonstrated that a complex methodological approach can be used to assess marine water areas, as well as to assess the biological conditions of invertebrates adapting to different environmental and anthropogenic effects.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russian Federation, 159, Stoletya Vladivostoku Str., Vladivostok, 690022, Russia. sasha0788@inbox.ru.
authors.forename: Alexandra V
authors.initials: AV
authors.lastname: Seitkalieva
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russian Federation, 159, Stoletya Vladivostoku Str., Vladivostok, 690022, Russia.
authors.forename: Natalie I
authors.initials: NI
authors.lastname: Menzorova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russian Federation, 159, Stoletya Vladivostoku Str., Vladivostok, 690022, Russia.
authors.forename: Valerу A
authors.initials: VA
authors.lastname: Rasskazov
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000187:drug effects / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D004787:Environmental Pollution / Q000032:analysis*; D057075:Enzyme Assays / Q000379:methods; D007564:Japan; D008297:Male; D012623:Seawater / Q000737:chemistry*; D047269:Strongylocentrotus / Q000187:drug effects / Q000378:metabolism; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000032:analysis*; D060753:Water Quality
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D000469:Alkaline Phosphatase
keywords: Biomarkers; Enzyme inhibition assays; Mussel Crenomytilus grayanus; Pollution monitoring; Sea urchin eggs; Sperm cell toxicity test; Strongylocentrotus intermedius