title: Thermo-sensitive imprinted polymer embedded carbon dots using epitope approach.
abstract: A new type of thermo-sensitive receptor carbon dots/SiO2/molecularly imprinted polymer (CDs/SiO2/MIP) was prepared by surface imprinting procedure and the epitope approach. The synthetic CDs/SiO2/MIP was able to selectively capture target protein with fluorescence quenching via the special interaction between them and the recognition cavities. The receptor exhibited the linear fluorescence quenching to cytochrome c (cyt c) in the range of 0.1-40 μM, and the detection limit was 89 nM. The precision for five replicate detection of cyt c at 20 μM was 3.11%. Moreover, the receptor owned the temperature-sensitive element that allowed for swelling and shrinking in response to temperature changes to realize recognition of the target cytochrome c. The proposed strategy revealed the feasibility of fabrication of a thermo-sensitive imprinted polymer based on CDs and surface imprinting procedure and the epitope approach.
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Li
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Yun-Jing
authors.initials: YJ
authors.lastname: Yan
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China. Electronic address: wyli@nankai.edu.cn.
authors.forename: Wen-You
authors.initials: WY
authors.lastname: Li
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D045304:Cytochromes c / Q000276:immunology / Q000302:isolation & purification*; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000939:Epitopes; D011108:Polymers; D002244:Carbon; D012822:Silicon Dioxide; D045304:Cytochromes c
keywords: Carbon dots; Epitope approach; Fluorescent recognition; Molecularly imprinted polymer