title: Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots.
abstract: With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75 % of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of noncoding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long noncoding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. As a novel class of RNA transcripts, the expression level and splicing variants of lncRNAs are various. Northern blot analysis can help us learn about the identity, size, and abundance of lncRNAs. Here we describe how to use northern blot to determine lncRNA abundance and identify different splicing variants of a given lncRNA.
authors.affiliation: Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, 421 Curie Blvd., 1207 Biomedical Research Building II/III, Philadelphia, PA, 19104, USA.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Hu
authors.affiliation: Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, 421 Curie Blvd., 1207 Biomedical Research Building II/III, Philadelphia, PA, 19104, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, 421 Curie Blvd., 1207 Biomedical Research Building II/III, Philadelphia, PA, 19104, USA.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, 421 Curie Blvd., 1207 Biomedical Research Building II/III, Philadelphia, PA, 19104, USA.
authors.forename: Youyou
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
authors.forename: Chao-Xing
authors.initials: CX
authors.lastname: Yuan
authors.affiliation: Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.affiliation: Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, 421 Curie Blvd., 1207 Biomedical Research Building II/III, Philadelphia, PA, 19104, USA. linzhang@mail.med.upenn.edu.|Department of Obstetrics and Gynecology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. linzhang@mail.med.upenn.edu.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D015152:Blotting, Northern / Q000379:methods*; D015870:Gene Expression; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D015341:Nucleic Acid Probes / Q000032:analysis / Q000235:genetics; D012326:RNA Splicing; D062085:RNA, Long Noncoding / Q000032:analysis* / Q000235:genetics
chemical_list: D015341:Nucleic Acid Probes; D062085:RNA, Long Noncoding
keywords: Long noncoding RNA; Northern blots; RNA expression