title: Effect of inorganic carbon on nitrogen removal and microbial communities of CANON process in a membrane bioreactor.
abstract: In this study, a membrane bioreactor (MBR) was adopted for completely autotrophic nitrogen removal over nitrite (CANON) process. Inorganic carbon (IC) was step-wise decreased to analyze the IC influence on nitrogen removal and microbial communities, finally IC was elevated to study its recovery capability. The bioactivities of functional organisms were detected by batch experiments. Results showed that the bioactivity and biodiversity of aerobic ammonia-oxidizing bacteria (AOB) and anaerobic ammonia-oxidizing bacteria (AAOB) both decreased due to the IC shortage, while nitrite-oxidizing bacteria bioactivity showed a contrary result. When the concentration ratio of IC to nitrogen (IC/N) decreased to 1.0, the nitrogen removal sharply deteriorated, which then recovered when the ratio increased to 2.5. Denaturing gradient gel electrophoresis results showed that Nitrosomonas sp. of AOB and Candidatus Brocadia fulgida of AAOB could survive in the condition of IC deficit. The prominent IC/N ratio for high-rate and stable CANON was between 1.5-2.0.
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, China.
authors.forename: Boyang
authors.initials: B
authors.lastname: Yu
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, China.
authors.forename: Haojing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, China. Electronic address: zhangxiaojing@zzuli.edu.cn.
authors.forename: Hongzhong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D052818:Autotrophic Processes; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000302:isolation & purification*; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D007287:Inorganic Chemicals / Q000302:isolation & purification*; D008567:Membranes, Artificial; D009573:Nitrites / Q000302:isolation & purification*; D009584:Nitrogen / Q000302:isolation & purification*; D010084:Oxidation-Reduction
chemical_list: D007287:Inorganic Chemicals; D008567:Membranes, Artificial; D009573:Nitrites; D002244:Carbon; D000641:Ammonia; D009584:Nitrogen
keywords: AAOB; CANON; Inorganic carbon (IC); Membrane bioreactor (MBR); Nitrogen removal