title: [Expression of plateau adaptation gene of rat tissues after plain acute exposure to high altitude].
abstract: To detect the expression of the plateau adaptablity gene(EPAS1, EGLN1 and PPARα) and proteins(HIF-2, PHD2 and PPARα) in rats blood, heart, liver, lung and kidney tissue after the rats exposed to high altitude.
  
  
  
  The Wistar rats were randomly divided into plain group(Shanghai, 55 m), acute exposure to high altitude 3400 m group, acute exposure to high altitude 4300 m group. Blood and organs of rats were collected in 1, 3, 5 days after arrival. Real time PCR and ELISA were used to compare the expression of plateau adaptablity gene and related protein between plain group and high altitude exposure groups.
  
  
  
  The count of red blood cells, hemoglobin and HCT in high altitude 4300 m were higher than those in plain group. Compared with plain group, the expression of EPAS1 gene in blood, heart, liver and kidney tissue of rats at high altitude increased obviously(all P<0.05); the expression of EGLN1 in the heart, liver, brain and kidney increased, and PPARα gene in the heart, liver and kidney increased(all P<0.05). Compared with plain group, the expression of HIF-2 protein increased significantly at high altitudes in the liver, brain and kidney tissues. PHD2 and PPARα increased in the heart, liver and kidney.
  
  
  
  Plateau adaptive genes(EPAS1, EGLN1 and PPARα) and protein(HIF-2, PHD2 and PPARα) differed in different altitude and different organizations. They might be used as target markers of plateau hypoxia.
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China;School of Life Science, Lanzhou University, Lanzhou 730050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Wen-bing
authors.initials: WB
authors.lastname: Li
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Zheng-ping
authors.initials: ZP
authors.lastname: Jia
authors.affiliation: Key Laboratory of Plateau Environmental Damage Control of PLA, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
mesh_terms: D000222:Adaptation, Physiological; D000531:Altitude; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D001921:Brain; D002681:China; D006321:Heart; D000860:Hypoxia; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases / Q000378:metabolism; D007668:Kidney; D008099:Liver; D008168:Lung; D047493:PPAR alpha / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases; D047493:PPAR alpha; D011393:Procollagen-Proline Dioxygenase; D000099162:Endothelial PAS Domain-Containing Protein 1; C580731:Egln1 protein, rat