title: [Effect of Interaction between PLIN Gene Polymorphisms and Open Lifestyle Intervention on Weight-loss in Chinese Han Adults].
abstract: OBJECTIVE
  To explore the effect of the interaction between PLIN gene polymorphisms and open lifestyle intervention on weight-loss in Chinese Han adults.
  
  
  METHODS
  Totally 109 overweight or obese subjects were assigned by random number table to the intervention group (n=56) or control group (n=53),and subjects in the intervention group received 22-week open lifestyle intervention. Anthropometric and metabolic indicators were measured for all subjects before and after intervention,and the PLIN1,PLIN4,and PLIN6 genotypes were amplified by polymerase chain reaction and sequenced through the first-generation sequencing technologies.
  
  
  RESULTS
  Among all these subjects,the rare allele C was dominant at PLIN1 (0.619),the common allele G was dominant at PLIN4 (0.606),and the common allele A was dominant at PLIN6 (0.564),in which PLIN1 and PLIN4 as well as PLIN4 and PLIN6 were in strong linkage disequilibrium (D'>0.9). After intervention,the body mass index,waist circumference,and body fat percent of female subjects were significantly decreased in intervention group and were lower than in control group;in male subjects,however,only the waist circumference showed significant difference with the control group (P<0.05). Subjects carrying rare allele homozygote of PLIN6 got less weight/fat loss than those carrying common alleles in intervention group,while subjects carrying rare allele of PLIN1 had more weight/fat increase than those with common allele homozygote in control group (P<0.05). Females in intervention group carrying any one of rare allele homozygotes of PLIN1,PLIN4 and PLIN6 got less weight/fat loss than those with common alleles,and female subjects carrying the rare allele homozygote haplotype of PLIN1/PLIN4 or PLIN4/PLIN6 got less weight/fat loss than those with other haplotypes (P<0.05).
  
  
  CONCLUSIONS
  The interaction between open lifestyle intervention and PLIN gene polymorphisms can directly influence weight-loss in Chinese Han overweight and obese adults.
authors.affiliation: Institute of Sports Medicine,Peking University Third Hospital,Beijing 100191,China.
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Lü
authors.affiliation: Institute of Sports Medicine,Peking University Third Hospital,Beijing 100191,China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Sports Medicine,Peking University Third Hospital,Beijing 100191,China.
authors.forename: Sha-sha
authors.initials: SS
authors.lastname: Jin
authors.affiliation: Institute of Sports Medicine,Peking University Third Hospital,Beijing 100191,China.
authors.forename: Xian-ren
authors.initials: XR
authors.lastname: Huang
authors.affiliation: Institute of Child and Adolescent Health,School of Public Health,Peking University,Beijing 100191,China.
authors.forename: Hai-jun
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: Clinical Research Institute,Peking University,Beijing 100191,China.
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of Medicine,Duke University Medical Center,Durham,North Carolina 27706,USA.
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Lin
authors.affiliation: Institute of Sports Medicine,Peking University Third Hospital,Beijing 100191,China.
authors.forename: Cui-qing
authors.initials: CQ
authors.lastname: Chang
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D000483:Alleles; D015992:Body Mass Index; D002352:Carrier Proteins; D005260:Female; D005838:Genotype; D006801:Humans; D008019:Life Style; D015810:Linkage Disequilibrium; D008297:Male; D009765:Obesity; D010750:Phosphoproteins; D011110:Polymorphism, Genetic; D055105:Waist Circumference; D015431:Weight Loss
chemical_list: D002352:Carrier Proteins; D010750:Phosphoproteins