title: A quantitative evaluation of ethylene production in the recombinant cyanobacterium Synechocystis sp. PCC 6803 harboring the ethylene-forming enzyme by membrane inlet mass spectrometry.
abstract: The prediction of the world's future energy consumption and global climate change makes it desirable to identify new technologies to replace or augment fossil fuels by environmentally sustainable alternatives. One appealing sustainable energy concept is harvesting solar energy via photosynthesis coupled to conversion of CO2 into chemical feedstock and fuel. In this work, the production of ethylene, the most widely used petrochemical produced exclusively from fossil fuels, in the model cyanobacterium Synechocystis sp. PCC 6803 is studied. A novel instrumentation setup for quantitative monitoring of ethylene production using a combination of flat-panel photobioreactor coupled to a membrane-inlet mass spectrometer is introduced. Carbon partitioning is estimated using a quantitative model of cyanobacterial metabolism. The results show that ethylene is produced under a wide range of light intensities with an optimum at modest irradiances. The results allow production conditions to be optimized in a highly controlled setup.
authors.affiliation: Department of Adaptive Biotechnologies, Global Change Research Centre, Academy of Science of the Czech Republic, Drásov, Czech Republic. Electronic address: zavrel.t@czechglobe.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Zavřel
authors.affiliation: Institut für Theoretische Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Knoop
authors.affiliation: Institut für Theoretische Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Steuer
authors.affiliation: Department of Life Sciences, Imperial College London, London, United Kingdom.
authors.forename: Patrik R
authors.initials: PR
authors.lastname: Jones
authors.affiliation: Department of Adaptive Biotechnologies, Global Change Research Centre, Academy of Science of the Czech Republic, Drásov, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Červený
authors.affiliation: Photon Systems Instruments, spol. s r.o., Drásov, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Trtílek
mesh_terms: D052818:Autotrophic Processes; D002244:Carbon / Q000032:analysis; D005030:Ethylenes / Q000096:biosynthesis*; D008027:Light; D008190:Lyases / Q000378:metabolism*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008567:Membranes, Artificial; D053858:Metabolic Networks and Pathways; D010100:Oxygen / Q000032:analysis; D011995:Recombination, Genetic / Q000235:genetics*; D046939:Synechocystis / Q000201:enzymology* / Q000254:growth & development / Q000528:radiation effects
chemical_list: D005030:Ethylenes; D008567:Membranes, Artificial; D002244:Carbon; C036216:ethylene; D008190:Lyases; C034693:ethylene forming enzyme; D010100:Oxygen
keywords: Biofuels; Biotechnology; Cyanobacteria; MIMS; Photobioreactor