title: [Effect of Astragalus mongholicus polysaccharides on gene expression profiles of dendritic cells isolated from healthy donors].
abstract: OBJECTIVE
  To investigate the anti-atherosclerosis mechanism of Astragalus mongholicus polysaccharides (APS) by examining its effect on gene expression profiles of the dendritic cells (DCs) from healthy donors.
  
  
  METHODS
  Peripheral blood DCs from healthy donors were incubated with 200 mg/L APS overnight, and changes in the gene expression profiles were investigated using microarray technique and RT-PCR.
  
  
  RESULTS
  Compared with the control cells, APS-treated DCs showed significantly up-regulated expressions of CD36 (0.97 ± 0.23 vs 5.45 ± 1.14) and IL-27 (1.08 ± 0.22 vs 2.97 ± 0.61) and down-regulated expression of expression of IFI16 (0.98 ± 0.18 vs 0.46 ± 0.11).
  
  
  CONCLUSIONS
  APS can promote the maturation and differentiation of DCs by up-regulating CD36 and IL-27 and down-regulating IFI16, and thus positively affects the occurrence and progression of the atherosclerosis.
authors.affiliation: Department of Neurology, Guangzhou Hospital of Integrated Chinese and Western Medicine, Guangzhou 510800, China. E-mail: cgzccj@163.com.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Li
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D018955:CD36 Antigens / Q000378:metabolism; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000187:drug effects*; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D018955:CD36 Antigens; D007378:Interleukins; C464565:MYDGF protein, human; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011134:Polysaccharides; C076700:IFI16 protein, human