title: The Proteomic Response of Arabidopsis thaliana to Cadmium Sulfide Quantum Dots, and Its Correlation with the Transcriptomic Response.
abstract: A fuller understanding of the interaction between plants and engineered nanomaterials is of topical relevance because the latter are beginning to find applications in agriculture and the food industry. There is a growing need to establish objective safety criteria for their use. The recognition of two independent Arabidopsis thaliana mutants displaying a greater level of tolerance than the wild type plant to exposure to cadmium sulfide quantum dots (CdS QDs) has offered the opportunity to characterize the tolerance response at the physiological, transcriptomic, and proteomic levels. Here, a proteomics-based comparison confirmed the conclusions drawn from an earlier transcriptomic analysis that the two mutants responded to CdS QD exposure differently both to the wild type and to each other. Just over half of the proteomic changes mirrored documented changes at the level of gene transcription, but a substantial number of transcript/gene product pairs were altered in the opposite direction. An interpretation of the discrepancies is given, along with some considerations regarding the use and significance of -omics when monitoring the potential toxicity of ENMs for health and environment.
authors.affiliation: Department of Life Sciences, University of Parma Parma, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Marmiroli
authors.affiliation: Department of Life Sciences, University of Parma Parma, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Imperiale
authors.affiliation: Department of Life Sciences, University of Parma Parma, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Pagano
authors.affiliation: Institute of Materials for Electronics and Magnetism (IMEM-CNR) Parma, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Villani
authors.affiliation: Institute of Materials for Electronics and Magnetism (IMEM-CNR) Parma, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Zappettini
authors.affiliation: Department of Life Sciences, University of Parma Parma, Italy.
authors.forename: Nelson
authors.initials: N
authors.lastname: Marmiroli
keywords: comparative analysis; engineered nanomaterials (ENM); exposure markers; genotoxicology ecotoxicology; tolerant mutants