title: Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification.
abstract: A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.
authors.affiliation: Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
authors.forename: Jianchang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, 38 Lingyusi Street, Baoding, 071001, Hebei, China.
authors.forename: Ruiwen
authors.initials: R
authors.lastname: Li
authors.affiliation: Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, 38 Lingyusi Street, Baoding, 071001, Hebei, China. yuanwanzhe2015@126.com.
authors.forename: Wanzhe
authors.initials: W
authors.lastname: Yuan
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000235:genetics*; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D017993:Parvovirus, Canine / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004279:DNA, Viral
keywords: Canine parvovirus type 2; Molecular diagnosis; Polymerase chain reaction; Recombinase polymerase amplification