title: The BioFilm Ring Test: a Rapid Method for Routine Analysis of Pseudomonas aeruginosa Biofilm Formation Kinetics.
abstract: Currently, few techniques are available for the evaluation of bacterial biofilm adhesion. These detection tools generally require time for culture and/or arduous handling steps. In this work, the BioFilm Ring Test (BRT), a new technology, was used to estimate the biofilm formation kinetics of 25 strains of Pseudomonas aeruginosa, isolated from the sputum of cystic fibrosis (CF) patients. The principle of the new assay is based on the mobility measurement of magnetic microbeads mixed with a bacterial suspension in a polystyrene microplate. If free to move under the magnetic action, particles gather to a visible central spot in the well bottom. Therefore, the absence of spot formation in the plate reflects the bead immobilization by a biofilm in formation. The BRT device allowed us to classify the bacterial strains into three general adhesion profiles. Group 1 consists of bacteria, which are able to form a solid biofilm in <2 h. Group 2 comprises the strains that progressively set up a biofilm during 24 h. Lastly, group 3 includes the strains that stay in a planktonic form. The grouping of our strains did not differ according to culture conditions, i.e., the use of different sets of beads or culture media. The BRT is shown to be an informative tool for the characterization of biofilm-forming bacteria. Various application perspectives may be investigated for this device, such as the addition of antibiotics to the bacterial suspension to select which would have the ability to inhibit the biofilm formation.
authors.affiliation: Fédération de médecine translationnelle, EA7290 Virulence bactérienne précoce, Institut de Bactériologie, Université de Strasbourg, Strasbourg, France BioFilm Control SAS, Saint-Beauzire, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Olivares
authors.affiliation: BioFilm Control SAS, Saint-Beauzire, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Badel-Berchoux
authors.affiliation: BioFilm Control SAS, Saint-Beauzire, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Provot
authors.affiliation: Fédération de médecine translationnelle, EA7290 Virulence bactérienne précoce, Institut de Bactériologie, Université de Strasbourg, Strasbourg, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Jaulhac
authors.affiliation: Fédération de médecine translationnelle, EA7290 Virulence bactérienne précoce, Institut de Bactériologie, Université de Strasbourg, Strasbourg, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Prévost
authors.affiliation: BioFilm Control SAS, Saint-Beauzire, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Bernardi
authors.affiliation: Fédération de médecine translationnelle, EA7290 Virulence bactérienne précoce, Institut de Bactériologie, Université de Strasbourg, Strasbourg, France jehl@unistra.fr.
authors.forename: François
authors.initials: F
authors.lastname: Jehl
mesh_terms: D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D018441:Biofilms; D003550:Cystic Fibrosis / Q000150:complications; D006801:Humans; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000502:physiology*; D013183:Sputum / Q000382:microbiology