title: Aptamer-functionalized stir bar sorptive extraction coupled with gas chromatography-mass spectrometry for selective enrichment and determination of polychlorinated biphenyls in fish samples.
abstract: In this paper, a novel aptamer-functionalized stir bar sorptive extraction (SBSE) was developed for selective enrichment of the low abundance polychlorinated biphenyls (PCBs) from fish samples. This approach was based on the immobilization of aptamer which could recognize 2,3',5,5'-tetrachlorobiphenyl (PCB72) and 2',3',4',5,5'-pentachlorobiphenyl (PCB106) on one kind of metal-organic frameworks (Zn4O(BDC)3, MOF-5). MOF-5 as a substrate was prepared by potential-controlled cathodic electrodeposition on stainless steel. This aptamer-functionalized stir bar sorptive extraction (Apt-MOF SBSE) fiber could be facile synthesized in one-step. PCB72 and PCB106 were employed as target analytes for selective extraction by the developed method. The adsorbed targets could be desorbed easily in pH 3.0 100mM glycine-HCl buffers and then extracted by the methylene chloride. Afterwards, the detection was carried out with gas chromatography-tandem mass spectrometry (GC-MS). The Apt-MOF SBSE pretreatment coupled with GC-MS exhibited high selectivity, good binding capacity, stability and reproducibility for the detection of PCBs. It provided a linear range of 0.02-250ngmL(-1) with a good coefficient of determination (R(2)=0.9991-0.9996) and the detection limit was 0.003-0.004ngmL(-1). More importantly, the method was successfully utilized for the determination of PCBs in fish samples with good enrichment factor (1930-2304). Therefore, this new SBSE coating opens up the possibility of selective enrichment of a given target PCBs from complex fish samples.
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Saichai
authors.initials: S
authors.lastname: Lin
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Faculty of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Deptartment of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance & Economics, Nanjing, China.
authors.forename: Yinji
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000327:Adsorption; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004784:Environmental Monitoring; D005399:Fishes; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D009942:Organometallic Compounds / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D009942:Organometallic Compounds; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D015032:Zinc
keywords: Aptamer; GC–MS; Metal-organic frameworks; Polychlorinated biphenyls; Stir bar sorptive extraction