title: Dynamic methylation pattern of cyp19a1a core promoter during zebrafish ovarian folliculogenesis.
abstract: In vertebrates, the aromatase coded by the cyp19a1a gene can catalyze the conversion from androgens to estrogens. Thus, the regulatory mechanisms of cyp19a1a gene expression are a critical research field in reproductive endocrinology. In this study, we use zebrafish as a model to study the dynamic methylation levels of the cyp19a1a gene core promoter during zebrafish ovarian folliculogenesis. The results show that there is an apparent fluctuation of the methylation levels of zebrafish cyp19a1a core promoter. Moreover, the methylation levels are inversely correlated with the expression levels of cyp19a1a transcripts when the ovarian follicles develop from PV into the MV stage. Also, the CpG dinucleotides which are close to the transcriptional starting site may have provided a significant blocking effect on inhibiting the transcriptional function of RNA polymerase II. Taken together, the results from the present study strongly suggest that DNA methylation was one of mechanisms that are involved in the regulation of cyp19a1a gene expression during folliculogenesis. This methylation mechanism modifying transcriptional process accompanied with zebrafish ovarian folliculogenesis might also shed new light on the regulation of cyp19a1a expression during the ovarian developmental stage in other vertebrates.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Bai
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Weida
authors.initials: W
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China.|College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Cuili
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Yida
authors.initials: Y
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China.|College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.|Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen, 361102, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, China.
authors.forename: Wanshu
authors.initials: W
authors.lastname: Hong
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, China. chenshixi@xmu.edu.cn.|College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China. chenshixi@xmu.edu.cn.|Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen, 361102, China. chenshixi@xmu.edu.cn.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, China. chenshixi@xmu.edu.cn.
authors.forename: Shi Xi
authors.initials: SX
authors.lastname: Chen
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D029961:Zebrafish Proteins; D001141:Aromatase
keywords: DNA methylation; Ovarian folliculogenesis; Zebrafish (Danio rerio); cyp19a1a gene