title: Preparation of the fast setting and degrading Ca-Si-Mg cement with both odontogenesis and angiogenesis differentiation of human periodontal ligament cells.
abstract: Develop a fast setting and controllable degrading magnesium-calcium silicate cement (Mg-CS) by sol-gel, and establish a mechanism using Mg ions to stimulate human periodontal ligament cells (hPDLs) are two purposes of this study. We have used the diametral tensile strength measurement to obtain the mechanical strength and stability of Mg-CS cement; in addition, the cement degradation properties is realized by measuring the releasing amount of Si and Mg ions in the simulated body fluid. The other cell characteristics of hPDLs, such as proliferation, differentiation and mineralization were examined while hPDLs were cultured on specimen surfaces. This study found out the degradation rate of Mg-CS cements depends on the Mg content in CS. Regarding in vitro bioactivity; the CS cements were covered with abundant clusters of apatite spherulites after immersion of 24h, while less apatite spherulites were formatted on the Mg-rich cement surfaces. In addition, the authors also explored the effects of Mg ions on the odontogenesis and angiogenesis differentiation of hPDLs in comparison with CS cement. The proliferation, alkaline phosphatase, odontogenesis-related genes (DSPP and DMP-1), and angiogenesis-related protein (vWF and ang-1) secretion of hPDLs were significantly stimulated when the Mg content of the specimen was increased. The results in this study suggest that Mg-CS materials with this modified composition could stimulate hPDLs behavior and can be good bioceramics for bone substitutes and hard tissue regeneration applications as they stimulate odontogenesis/angiogenesis.
authors.affiliation: Graduate Institute of Clinical Medical Science, China Medical University, Taichung City, Taiwan; 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Institute of Oral Science, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Tuan-Ti
authors.initials: TT
authors.lastname: Hsu
authors.affiliation: H. Milton Stewart School of Industrial and Systems Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Georgia Tech Manufacturing Institute, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D000293:Adolescent; D000328:Adult; D017610:Calcium Compounds / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008274:Magnesium / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009805:Odontogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology*; D017640:Silicates / Q000737:chemistry*; D055815:Young Adult
chemical_list: D017610:Calcium Compounds; D003738:Dental Cements; D017640:Silicates; D008274:Magnesium; C031293:calcium silicate
keywords: Angiogenic; Biodegradable; Calcium silicate; Magnesium; Odontogenic; Periodontal ligament cells