title: Methanol extracts from Cystoseira tamariscifolia and Cystoseira nodicaulis are able to inhibit cholinesterases and protect a human dopaminergic cell line from hydrogen peroxide-induced cytotoxicity.
abstract: Context Marine macroalgae contain several bioactive molecules that may be developed as functional foods, but information about their neuroprotective potential is scarce. Objective The objective of this study is to determine the in vitro antioxidant and neuroprotective features of marine algae from the southern coast of Portugal and to assess the total content of different types of bioactives. Materials and methods Methanol extracts from 21 macroalgal species from the southern Portugal were evaluated for in vitro antioxidant and acetylcholinesterase (AChE) inhibition. Active extracts were further evaluated for inhibitory activity against butyrylcholinesterase (BuChE) and tyrosinase (TYRO), and for their ability to attenuate hydrogen peroxide (H2O2)-induced toxicity in SH-SY5Y cells. The total contents of different phenolic groups were determined for the most active extracts. Results Cystoseira tamariscifolia (Hudson) Papenfuss (Sargassaceae) had the highest antiradical activity (92%, 1 mg/mL). Cystoseira nodicaulis (Withering) M. Roberts (Sargassaceae) (75%) and Cystoseira humilis Schousboe ex Kützing (Sargassaceae) (70%) had the highest iron-chelating activity at 10 mg/mL. Cystoseira baccata (S.G. Gmelin) P.C. Silva (Sargassaceae) was more active towards copper (66%, 10 mg/mL). Cystoseira tamariscifolia had the highest AChE inhibitory capacity (85%, 10 mg/mL). Cystoseira tamariscifolia and C. nodicaulis were also active against BuChE and TYRO, and were able to protect SH-SY5Y cells against oxidative stress induced by H2O2. Cystoseira tamariscifolia had the highest content of all the groups of phenolics, and was particularly enriched in hydroxycinnamic acids (106 mg CAE/g DW). Discussion and conclusion Results indicate that C. tamariscifolia and C. nodicaulis are important sources of nutraceutical compounds and may be considered functional foods that could improve cognitive functions.
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Custódio
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Laura
authors.initials: L
authors.lastname: Silvestre
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Rocha
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Rodrigues
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Vizetto-Duarte
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Hugo
authors.initials: H
authors.lastname: Pereira
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Barreira
authors.affiliation: a Center of Marine Sciences, University of Algarve, Faculty of Sciences and Technology , Faro , Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Varela
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002091:Butyrylcholinesterase / Q000378:metabolism*; D045744:Cell Line, Tumor; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D003373:Coumaric Acids / Q000302:isolation & purification / Q000494:pharmacology; D019610:Cytoprotection; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D000432:Methanol / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D012624:Seaweed / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002800:Cholinesterase Inhibitors; D003373:Coumaric Acids; D058851:GPI-Linked Proteins; D018696:Neuroprotective Agents; D010636:Phenols; D010851:Picrates; D012997:Solvents; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; C548244:ACHE protein, human; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000432:Methanol
keywords: Acetylcholinesterase; antioxidants; butyrylcholinesterase; functional foods; neuroprotection; nutraceuticals; tyrosinase