title: Restriction of protein synthesis abolishes senescence features at cellular and organismal levels.
abstract: Cellular senescence or its equivalence is induced by treatment of cells with an appropriate inducer of senescence in various cell types. Mild restriction of cytoplasmic protein synthesis prevented induction of all aspects of cellular senescence in normal and tumor-derived human cells. It allowed the cells to continuously grow with no sign of senescent features in the presence of various inducers. It also delayed replicative senescence in normal human fibroblasts. Moreover, it allowed for growth of the cells that had entered a senescent state. When adult worms of the nematode C. elegans were grown under protein-restricted conditions, their average and maximal lifespans were significantly extended. These results suggest that accumulation of cytoplasmic proteins due to imbalance in macromolecule synthesis is a fundamental cause of cellular senescence.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takauji
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Wada
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Takeda
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Ikuru
authors.initials: I
authors.lastname: Kudo
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.|Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama, Kanagawa 231-0048, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Miki
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.|Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama, Kanagawa 231-0048, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D000375:Aging; D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D008136:Longevity / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects
chemical_list: D003513:Cycloheximide; D020928:Mitogen-Activated Protein Kinases