title: Evaluation of gene expression level of CDC5L and MACC1 in poor prognosis and progression of osteosarcoma.
abstract: Current evidences have indicated that osteosarcoma is strongly associated with abnormal genetic and epigenetic changes that lead to the abnormal expression of oncogenes or methylation of tumor suppressor genes. In the present study, MACC1 and CDC5L mRNA levels in the patients with osteosarcoma were evaluated using quantitative real-time PCR. Our results demonstrated that CDC5L mRNA levels were higher in tumor tissues than in adjacent normal tissues (2.713 ± 0.738 vs. 1.071 ± 0.629; P < 0.05). Moreover, MACC1 was upregulated in tumor bone tissues than in adjacent normal tissues (3.221 ± 0. 624 vs. 1.427 ± 0.456; P < 0.05). Our result demonstrated that high expression of CDC5L was significantly related to advanced TNM stage (P = 0.032). No significant difference was determined between CDC5L mRNA expression and other clinicopathological parameters including age, gender, tumor diameter, location, tumor grade, and histological type. In addition, overexpression of MACC1 was strongly correlated with advanced TNM stage (P = 0.027) and high tumor grade (P = 0.035). Our findings indicated that mRNA level of CDC5L is correlated with advanced TNM stage, and MACC1 may be involved in progression of osteosarcoma.
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Department of Neurosurgery, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Peyman Karimi
authors.initials: PK
authors.lastname: Goudarzi
authors.affiliation: Department of Pathology, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Rahmani
authors.affiliation: Department of Orthopedic and Traumatology, Universitätsklinikum Bonn, Bonn, Germany.
authors.forename: Peyman
authors.initials: P
authors.lastname: Kaghazian
authors.affiliation: Young Researchers and Elite Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.affiliation: Department of Orthopedic Surgery, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Taheriazam
authors.affiliation: Department of Emergency Medicine, Alborz University of Medical Sciences, Karaj, Iran. ahmadik@mums.ac.ir.
authors.forename: Koroosh
authors.initials: K
authors.lastname: Ahmadi
keywords: CDC5L; Expression; MACC1; Osteosarcoma; PCR