title: The complete chloroplast genome sequence of Indian mustard (Brassica juncea L.).
abstract: Brassica juncea L. is the second most important edible oil seed crop of India. In the current study, we report the complete chloroplast genome sequence of Indian mustard, Brassica juncea L. The size of the complete chloroplast genome was found to be 153 483 bp long with an overall GC content of 36.36%. A large single copy (LSC) region of 83 286 bp and a short single copy region (SSC) region of 17 775 bp were separated by a pair of inverted repeat (IR) regions of 26 211 bp. A total of 113 unique genes were annotated, which includes 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Either one or two introns were present in nine protein-coding genes and six tRNA genes. Phylogenetic analysis with the complete chloroplast genomes of other related species revealed that B. juncea is much closer to Brassica rapa.
authors.affiliation: a Department of Genetic Engineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Sudheesh K
authors.initials: SK
authors.lastname: Prabhudas
authors.affiliation: a Department of Genetic Engineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Balaji
authors.initials: B
authors.lastname: Raju
authors.affiliation: a Department of Genetic Engineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Shahid
authors.initials: S
authors.lastname: Kannan Thodi
authors.affiliation: a Department of Genetic Engineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Madasamy
authors.initials: M
authors.lastname: Parani
authors.affiliation: a Department of Genetic Engineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Purushothaman
authors.initials: P
authors.lastname: Natarajan
mesh_terms: D002736:Chloroplasts / Q000235:genetics*; D018742:DNA, Chloroplast / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054628:Genome, Chloroplast; D007194:India; D007438:Introns; D055029:Inverted Repeat Sequences / Q000235:genetics; D009149:Mustard Plant / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018742:DNA, Chloroplast; D010940:Plant Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Brassica; Brassicaceae; Complete chloroplast genome; De novo assembly; mustard