title: Perchlorate reduction by hydrogen autotrophic bacteria and microbial community analysis using high-throughput sequencing.
abstract: Hydrogen autotrophic reduction of perchlorate have advantages of high removal efficiency and harmless to drinking water. But so far the reported information about the microbial community structure was comparatively limited, changes in the biodiversity and the dominant bacteria during acclimation process required detailed study. In this study, perchlorate-reducing hydrogen autotrophic bacteria were acclimated by hydrogen aeration from activated sludge. For the first time, high-throughput sequencing was applied to analyze changes in biodiversity and the dominant bacteria during acclimation process. The Michaelis-Menten model described the perchlorate reduction kinetics well. Model parameters q(max) and K(s) were 2.521-3.245 (mg ClO4(-)/gVSS h) and 5.44-8.23 (mg/l), respectively. Microbial perchlorate reduction occurred across at pH range 5.0-11.0; removal was highest at pH 9.0. The enriched mixed bacteria could use perchlorate, nitrate and sulfate as electron accepter, and the sequence of preference was: NO3(-) > ClO4(-) > SO4(2-). Compared to the feed culture, biodiversity decreased greatly during acclimation process, the microbial community structure gradually stabilized after 9 acclimation cycles. The Thauera genus related to Rhodocyclales was the dominated perchlorate reducing bacteria (PRB) in the mixed culture.
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.
authors.forename: Dongjin
authors.initials: D
authors.lastname: Wan
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.
authors.forename: Yongde
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China. xiaoshuhu@126.com.
authors.forename: Shuhu
authors.initials: S
authors.lastname: Xiao
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.
authors.forename: Daorong
authors.initials: D
authors.lastname: Li
mesh_terms: D052818:Autotrophic Processes; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D060766:Drinking Water; D059014:High-Throughput Nucleotide Sequencing; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015233:Models, Statistical; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000378:metabolism*; D012722:Sewage; D020628:Thauera / Q000235:genetics / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D060766:Drinking Water; D010472:Perchlorates; D012722:Sewage; D006859:Hydrogen; C494474:perchlorate
keywords: Autotrophic; High-throughput sequencing; Hydrogen; Perchlorate; Thauera genus