title: Circulating DNA and its methylation level in inflammatory bowel disease and related colon cancer.
abstract: Both of chronic inflammation and abnormal immune in inflammatory bowel disease can induce colon cancer. Previous research showed that cell apoptosis and necrosis become the main source of circulating DNA in the peripheral blood during tumorigenesis that reduced along with methylation degree. However, its role in the process of colitis transforming to colon cancer is not clarified. Drinking 3% DSS was used to establish colitis model, while 3% dextran sodium sulfate (DSS) combined with azo oxidation methane (AOM) intraperitoneal injection was applied to establish colitis related colon cancer model. Circulating DNA and its methylation level in peripheral blood were tested. Morphology observation, HE staining, and p53 and β-catenin expression detection confirmed that drinking 3% DSS and 3% DSS combined with AOM intraperitoneal injection can successfully establish colitis and colitis associated colorectal cancer models. Circulating DNA level in colitis and colon cancer mice increased by gradient compared with control, while significant difference was observed between each other. Circulating DNA methylation level decreased obviously in colitis and colon cancer, and significant difference was observed between each other. Abnormal protein expression, circulating DNA and its methylation level in ulcerative colitis associated colorectal tissues change in gradient, suggesting that circulating DNA and its methylation level can be treated as new markers for colitis cancer transformation that has certain significance to explore the mechanism of human ulcerative colitis canceration.
authors.affiliation: Department of Interventional Radiology, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, China.
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Clinical Oncology, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, China.
authors.forename: Wangyang
authors.initials: W
authors.lastname: Pu
authors.affiliation: The Molecular Laboratory Center, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: College of Pharmaceutical Science, Soochow University Suzhou 215000, Jiangsu, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University Suzhou 215000, Jiangsu, China.
authors.forename: Chungen
authors.initials: C
authors.lastname: Xing
authors.affiliation: Department of Interventional Radiology, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation; D015212:Inflammatory Bowel Diseases / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53; D051176:beta Catenin; D004247:DNA; D016264:Dextran Sulfate
keywords: Circulating DNA; colitis; colon cancer; methylation