title: Synergism of proteomics and mRNA sequencing for enzyme discovery.
abstract: Enzyme catalyzed processes are increasingly complementing chemical manufacturing as new enzymes are being discovered. Although, many industrially applied biocatalysts have been identified by functional screenings technological advances in the omics fields have created a different path to access novelty. Here we describe how omics technologies, especially proteomics and transcriptomics, can complement each other in the aim of finding new enzymatic functions. Special emphasis is laid on how mRNA sequencing Zcan improve proteomic experiments by allowing the generation of high quality protein sequence databases, which subsequently facilitates protein identification.
authors.affiliation: Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria.
authors.forename: Lukas
authors.initials: L
authors.lastname: Sturmberger
authors.affiliation: Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; Medical University of Graz, Institute of Pathology, Research Unit Functional Proteomics and Metabolic Pathways, Stiftingtalstrasse 24, 8010 Graz, Austria; Omics Center Graz, BioTechMed-Graz, Stiftingtalstrasse 24, 8010 Graz, Austria.
authors.forename: Paal W
authors.initials: PW
authors.lastname: Wallace
authors.affiliation: Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; TU Graz, Institute of Molecular Biotechnology, NAWI Graz, Petersgasse 14, 8010 Graz, Austria.
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
authors.affiliation: Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; Medical University of Graz, Institute of Pathology, Research Unit Functional Proteomics and Metabolic Pathways, Stiftingtalstrasse 24, 8010 Graz, Austria; Omics Center Graz, BioTechMed-Graz, Stiftingtalstrasse 24, 8010 Graz, Austria. Electronic address: ruth.birner-gruenberger@medunigraz.at.
authors.forename: Ruth
authors.initials: R
authors.lastname: Birner-Gruenberger
mesh_terms: D001709:Biotechnology; D030561:Databases, Nucleic Acid; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D004798:Enzymes; D012333:RNA, Messenger
keywords: Biotechnology; Database; Enzyme discovery; Proteomics; Transcriptomics