title: Pathologic changes of wound tissue in rats with stage III pressure ulcers treated by transplantation of human amniotic epithelial cells.
abstract: This study aims to determine the impact of orthotopic transplantation of human amniotic epithelial cells (hAECs) on the pathologic changes of wound tissues in a self-prepared rat stage III pressure ulcer model. Ninety-six SD rats were randomly divided into the model group (group M), hAEC transplantation group (group H), traditional treatment group (group T), and the control group (group C), with 24 rats in each group. The wound healing time was observed in 6 rats from each group, and 6 rats of each group were selected for post-modeling on day(s) (D) 1, 3, and 7 for HE staining to compare the pathological changes. The healing time of group H was significantly shorter than the other three groups. Moreover, pathological observations revealed that group H exhibited significant proliferation of fibrous tissues and vessels in the dermal layer, and the appearance time and degree of skin appendages were significantly greater than that observed in the other three groups. Pathological observations showed that hAEC transplantation could significantly speed up the healing of stage III pressure ulcer.
authors.affiliation: Nursing College, Zunyi Medical College Zunyi 563000, Guizhou, China.
authors.forename: Xilan
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Nursing College, Zunyi Medical College Zunyi 563000, Guizhou, China.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Nursing College, Zunyi Medical College Zunyi 563000, Guizhou, China.
authors.forename: Aiting
authors.initials: A
authors.lastname: Zhou
authors.affiliation: Cell Engineering Laboratory, The Affiliated Hospital of Zunyi Medical College Zunyi 563000, Guizhou, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Yu
authors.affiliation: Intensive Care Unit (ICU), The Affiliated Hospital of Zunyi Medical College Zunyi 563000, Guizhou, China.
authors.forename: Mingtao
authors.initials: M
authors.lastname: Quan
authors.affiliation: Clinical School, The Affiliated Hospital of Zunyi Medical College Zunyi 563000, Guizhou, China.
authors.forename: Huagang
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000650:Amnion / Q000637:transplantation*; D000818:Animals; D004847:Epithelial Cells / Q000637:transplantation; D006801:Humans; D008297:Male; D003668:Pressure Ulcer / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing
keywords: Human amniotic epithelial cells; pathology; stage III pressure ulcer