title: Inhibition of matrix metalloproteinases and toxicity of gold and platinum nanoparticles in L929 fibroblast cells.
abstract: This study evaluated the inhibition of matrix metalloproteases (MMPs) and cellular responses elicited by gold (Au) and platinum (Pt) nanoparticles (NPs). The interaction of MMP-1 and NPs was evaluated using an MMP assay kit. The cultured L929 cells were exposed to various concentrations of NPs. The cellular responses to NPs were examined using a cytotoxicity assay (that evaluated cell viability and lactic dehydrogenase production), real-time polymerase chain reaction (RT-qPCR), and transmission electron microscopy. Both types of NPs, when used at concentrations above 10 μg ml(-1), inhibited MMP-1 activity. No cytotoxic effects were found when the cells were exposed to AuNPs. In contrast, PtNPs, at both 100 and 400 μg ml(-1), induced cytotoxicity. No inflammatory responses (production of interleukin-6 and tumor necrosis factor-alpha) to NPs were identified by RT-qPCR. The negative surface charge of NPs (COOH(-)) binds to the Zn(2+) of the MMP active center by chelation, leading to MMP inhibition. Gold nanoparticles are plausible candidates for MMP inhibitors in resin-bonding materials because they effectively inhibit MMP-1 activity without cytotoxic or inflammatory effects.
authors.affiliation: Department of Biomaterials Science, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Biomaterials Science, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Department of Biomaterials Science, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Sasaki
authors.affiliation: Research & Development Department, Chemical Division, Miyoshi Oil & Fat, Horikiri Katsushika-ku, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kawai
authors.affiliation: Research & Development Department, Chemical Division, Miyoshi Oil & Fat, Horikiri Katsushika-ku, Tokyo, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.affiliation: Division of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.affiliation: Department of Biomaterials Science, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
mesh_terms: D002460:Cell Line; D005347:Fibroblasts; D006046:Gold; D020782:Matrix Metalloproteinases; D053758:Nanoparticles; D010984:Platinum
chemical_list: D010984:Platinum; D006046:Gold; D020782:Matrix Metalloproteinases
keywords: MMP; cytotoxicity; fibroblasts; nanoparticle; resin