title: Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
abstract: Tools for genetically-determined visualization of synaptic circuits and interactions are necessary to build connectomics of the vertebrate brain and to screen synaptic properties in neurological disease models. Here we develop a transgenic FingR (fibronectin intrabodies generated by mRNA display) technology for monitoring synapses in live zebrafish. We demonstrate FingR labeling of defined excitatory and inhibitory synapses, and show FingR applicability for dissecting synapse dynamics in normal and disease states. Using our system we show that chronic hypoxia, associated with neurological defects in preterm birth, affects dopaminergic neuron synapse number depending on the developmental timing of hypoxia.
authors.affiliation: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Son
authors.affiliation: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Keefe
authors.affiliation: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Tamara J
authors.initials: TJ
authors.lastname: Stevenson
authors.affiliation: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.|Interdepartmental Program in Neurosciences, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Barrios
authors.affiliation: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Scott
authors.initials: S
authors.lastname: Anjewierden
authors.affiliation: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: James B
authors.initials: JB
authors.lastname: Newton
authors.affiliation: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.|Interdepartmental Program in Neurosciences, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Adam D
authors.initials: AD
authors.lastname: Douglass
authors.affiliation: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.|Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.|Interdepartmental Program in Neurosciences, University of Utah School of Medicine, Salt Lake City, Utah.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Bonkowsky
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D058948:Cell Tracking; D005353:Fibronectins / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D000860:Hypoxia / Q000378:metabolism; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D005353:Fibronectins