title: Cardiac Glycosides from the Seeds of Thevetia peruviana.
abstract: Investigation of the seeds of Thevetia peruviana resulted in the isolation of 15 new (2-16) and 18 known (1 and 17-33) cardiac glycosides. Eight 19-nor-cardenolides (1-8), including two rare 19-nor-10-hydroperoxycardenolides, were obtained from T. peruviana for the first time. All the structures were characterized by NMR spectroscopy and chemical derivatization. The inhibitory effects of cardiac glycosides 1-33 against three cancer cell lines (human lung cancer cells, P15; human gastric cancer cells, MGC-803; and human pancreatic cancer cells, SW1990) and one normal hepatocyte cell line, LO2, were evaluated, and a preliminary structure-activity relationship is discussed. In addition, cardiac glycosides 1, 22, 26, and 28 were evaluated for their apoptosis-inducing activities in MGC-803 cells, showing IC50 values in the range 0.02-0.53 μM.
authors.affiliation: College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Dan-Mei
authors.initials: DM
authors.lastname: Tian
authors.affiliation: Department of Anatomy, Medical College, Jinan University , Guangzhou 510632, People's Republic of China.
authors.forename: Huo-Yun
authors.initials: HY
authors.lastname: Cheng
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine , 88 Yuquan Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Jiang
authors.affiliation: Department of Anatomy, Medical College, Jinan University , Guangzhou 510632, People's Republic of China.
authors.forename: Wei-Zai
authors.initials: WZ
authors.lastname: Shen
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University , Guangzhou 510632, People's Republic of China.
authors.forename: Jin-Shan
authors.initials: JS
authors.lastname: Tang
authors.affiliation: College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002301:Cardiac Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012639:Seeds / Q000737:chemistry*; D013329:Structure-Activity Relationship; D031052:Thevetia / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D002301:Cardiac Glycosides; D004365:Drugs, Chinese Herbal