title: WNT-associated gene expression in human mesenchymal stromal cells under hypoxic stress.
abstract: The effect of short-term stress on the expression of 84 Wnt-related genes in mesenchymal stromal cells (MSCs) was analyzed. In MSCs permanently expanded at 5% O2, Dkk1, RHOA, CSNK1A1, CSNK2A1, GSK3β, and PORCN genes were most highly expressed. FZD1, FZD3, SFRP1, and SFRP4 genes were up-regulated after short-term hypoxic stress (0.1% O2), whereas the expression of LEF-1 and RUVBL1 genes was significantly inhibited (down-regulated). The data suggest that Wnt signal transduction was suppressed under hypoxic stress, whereas the noncanonical Wnt signaling prevailed under the "physiological hypoxia" (5% O2).
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe shosse 76-a, Moscow, 123007, Russia. olja_udartseva@rambler.ru.
authors.forename: O O
authors.initials: OO
authors.lastname: Udartseva
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe shosse 76-a, Moscow, 123007, Russia.
authors.forename: E R
authors.initials: ER
authors.lastname: Andreeva
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe shosse 76-a, Moscow, 123007, Russia.
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
mesh_terms: D015687:Cell Hypoxia; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D013312:Stress, Physiological; D060449:Wnt Signaling Pathway
chemical_list: D010100:Oxygen