title: [Isolation and identification of aerobic and facultative anaerobic bacteria in the oral cavity].
abstract: OBJECTIVE
  To establish a systematic method for isolation and identification of aerobic and facultative anaerobic bacteria in the oral cavity.
  
  
  METHODS
  Samples of the saliva, dental plaque and periapical granulation tissue were collected from 20 subjects with healthy oral condition and from 8 patients with different oral diseases. The bacteria in the samples were identified by morphological identification, VITEK automatic microorganism identification and 16s rRNA gene sequencing.
  
  
  RESULTS
  VITEK automatic microorganism identification and 16s rRNA gene sequencing showed an agreement rate of 22.39% in identifying the bacteria in the samples. We identified altogether 63 bacterial genus (175 species), among which Streptococcus, Actinomyces and Staphylococcus were the most common bacterial genus, and Streptococcus anginosus, Actinomyces oris, Streptococcus mutans and Streptococcus mitis were the most common species. Streptococcus anginosus was commonly found in patients with chronic periapical periodontitis. Streptococcus intermedius and Staphylococcus aureus were common in patients with radiation caries, and in patients with rampant caries, Streptococcus mutans was found at considerably higher rate than other species.
  
  
  CONCLUSIONS
  Aerobic and facultative anaerobic bacteria are commonly found in the oral cavity, and most of them are gram-positive. 16s rRNA gene sequencing is more accurate than VITEK automatic microorganism identification in identifying the bacteria.
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China. E-mail: 451009639@qq.com.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Lu
authors.forename: Fanzi
authors.initials: F
authors.lastname: Wu
authors.forename: Xinxuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Wu
authors.forename: Mingyun
authors.initials: M
authors.lastname: Li
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Ruijie
authors.initials: R
authors.lastname: Huang
authors.forename: Jiyao
authors.initials: J
authors.lastname: Li
authors.forename: Liying
authors.initials: L
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification*; D003731:Dental Caries; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012463:Saliva / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013291:Streptococcus / Q000302:isolation & purification*
chemical_list: D012336:RNA, Ribosomal, 16S