title: Redox-Responsive Porphyrin-Based Polysilsesquioxane Nanoparticles for Photodynamic Therapy of Cancer Cells.
abstract: The development of stimulus-responsive photosensitizer delivery systems that carry a high payload of photosensitizers is of great importance in photodynamic therapy. In this study, redox-responsive polysilsesquioxane nanoparticles (PSilQNPs) built by a reverse microemulsion approach using 5,10,15,20-tetrakis(carboxyphenyl) porphyrin (TCPP) silane derivatives as building blocks, were successfully fabricated. The structural properties of TCPP-PSilQNPs were characterized by dynamic light scattering (DLS)/ζ-potential, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The photophysical properties were determined by UV-vis and fluorescence spectroscopy. The quantity of singlet oxygen generated in solution was measured using 1,3-diphenylisobenzofuran. The redox-responsive release of TCPP molecules was successfully demonstrated in solution in the presence of a reducing agent. The internalization of TCPP-PSilQNPs in cancer cells was investigated using laser scanning confocal microscopy. Phototoxicity experiments in vitro showed that the redox-responsive TCPP-PSilQNPs exhibited an improved phototherapeutic effect on cervical cancer cells compared to a non-responsive TCPP-PSilQNP control material.
authors.affiliation: Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA. dvega1@uncc.edu.|The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA. dvega1@uncc.edu.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Vega
authors.affiliation: Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA. plodge@uncc.edu.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lodge
authors.affiliation: Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA. jviveroe@uncc.edu.|The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA. jviveroe@uncc.edu.
authors.forename: Juan L
authors.initials: JL
authors.lastname: Vivero-Escoto
mesh_terms: D003692:Delayed-Action Preparations / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008665:Metalloporphyrins / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D017646:Organosilicon Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: C503831:5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin cobalt; D003692:Delayed-Action Preparations; D008665:Metalloporphyrins; D017646:Organosilicon Compounds; D017319:Photosensitizing Agents; C569780:polysilsesquioxane
keywords: cancer therapy; photodynamic therapy; photosensitizer delivery; polysilsesquioxane nanoparticles; porphyrin; stimulus-responsive materials