title: Expression of ITGB1 predicts prognosis in colorectal cancer: a large prospective study based on tissue microarray.
abstract: BACKGROUND
  ITGB1 is a heterodimeric cell-surface receptor involved in cell functions such as proliferation, migration, invasion and survival. The aim of this study was to assess ITGB1 expression in colorectal cancer and correlate it with clinicopathological features, as well as to evaluate its potential prognostic significance.
  
  
  METHODS
  In this study, we examined the expression of ITGB1 using tissue microarrays containing analyzed specimens by immunohistochemistry. ITGB1 expression was further correlated with clinicopathological and prognostic data. The prognostic significance was assessed using Kaplan-Meier survival estimates and log-rank tests. A multivariate study with the Cox's proportional hazard model was used to evaluate the prognostic aspects.
  
  
  RESULTS
  ITGB1 expression was present in 88.5% of the analyzed specimens. Significant differences in ITGB1 expression were found between normal mucosa and carcinomas (P<0.001). High ITGB1 expression was associated with poor prognosis, and it independently correlated with shortened overall survival and disease-free survival in colorectal cancer patients (P<0.001). More so, ITGB1 expression, bowel wall invasion, lymph node metastasis and distant metastasis were independent prognostic factors for overall survival. Additionally, significant differences in ITGB1 expression were observed in adenomas and tumors from patients with familial adenomatous polyposis compared to normal colon mucosa (P<0.05) CONCLUSION: The results of this study indicate that ITGB1 overexpression in colorectal tumors is associated with poor prognosis, as well as aggressive clinicopathological features. Therefore, ITGB1 expression could be used as potential prognostic predictor in colorectal cancer patients.
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Liu
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Gao
authors.affiliation: Department of Epidemiology, Second Military Medical University Shanghai, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Chang
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Gong
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Chuan-Gang
authors.initials: CG
authors.lastname: Fu
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, Second Military Medical University Shanghai, China.
authors.forename: Guang-Wen
authors.initials: GW
authors.lastname: Cao
mesh_terms: D014408:Biomarkers, Tumor; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011446:Prospective Studies; D015996:Survival Rate; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D019012:Integrin beta1
keywords: Colorectal cancer; ITGB1; prognosis; tissue microarray