title: Fast determination of catecholamines in human plasma using carboxyl-functionalized magnetic-carbon nanotube molecularly imprinted polymer followed by liquid chromatography-tandem quadrupole mass spectrometry.
abstract: A novel, simple and sensitive method based on the use of dispersive micro-solid-phase extraction (d-μ-SPE) procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) for the determination of catecholamines, i.e., dopamine (DA), norepinephrine(NE) and epinephrine (E), was developed and validated. The novel catecholamines molecularly imprinted polymer (MIP) on the surface of carboxyl-functionalized magnetic-carbon nanotube (CF@m-CNTs-MIP) was synthesized and characterized by vibrating sample magnetometer (VSM), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The CF@m-CNTs-MIP was used as the d-μ-SPE sorbent to extract catecholamines from human plasma samples. The obtained results demonstrated the higher extraction capacity of CF@m-CNTs-MIP with recoveries between 87.5-110%. The limits of quantification (LOQs) for NE, E and DA were 76 ng/L, 18 ng/L and 10 ng/L, respectively. Validation results on linearity, specificity, accuracy, precision and stability, as well as on application to the analysis of catecholamines in 120 healthy volunteers demonstrated the applicability to clinical studies.
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang 315010, China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Ma
authors.affiliation: Ningbo First Hospital, Ningbo, Zhejiang 315010, China.
authors.forename: Hai-Wen
authors.initials: HW
authors.lastname: Qiu
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang 315010, China.
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Rui
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang 315010, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Liao
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang 315010, China.
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: Chen
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang 315010, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China. Electronic address: zhyg91213@163.com.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Zhao
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D002395:Catecholamines / Q000097:blood*; D002853:Chromatography, Liquid; D006801:Humans; D008280:Magnetics; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D002395:Catecholamines; D037742:Nanotubes, Carbon; D011108:Polymers
keywords: Dispersive micro-solid-phase extraction (d-μ-SPE); Dopamine; Epinephrine; Human plasma; Liquid chromatography–tandem quadrupole mass spectrometry (LC–MS/MS); Norepinephrine