title: Random sequential adsorption of human adenovirus 2 onto polyvinylidene fluoride surface influenced by extracellular polymeric substances.
abstract: Virus removal by membrane bioreactors depends on virus-membrane and virus-foulant interactions. The adsorption of human adenovirus 2 (HAdV-2) on polyvinylidene fluoride (PVDF) membrane and a major membrane foulant, extracellular polymeric substances (EPS), were measured in a quartz crystal microbalance. In 3-100mM CaCl2 solutions, irreversible adsorption of HAdV-2 was observed on both pristine and EPS-fouled PVDF surfaces. The HAdV-2 adsorption kinetics was successfully fitted with the random sequential adsorption (RSA) model. The applicability of the RSA model for HAdV-2 adsorption is confirmed by comparing the two fitting parameters, adsorption rate constant k(a) and area occupied by each adsorbed HAdV-2 particle a, with experimentally measured parameters. A linear correlation between the fitting parameter k(a) and the measured attachment efficiency was found, suggesting that the RSA model correctly describes the interaction forces dominating the HAdV-2 adsorption. By comparing the fitting parameter d(ads) with the hydrodynamic diameter of HAdV-2, we conclude that virus-virus and virus-surface interactions determine the area occupied by each adsorbed HAdV-2 particle, and thus influence the adsorption capacity. These results provide insights into virus retention and will benefit improving virus removal in membrane filtration.
authors.affiliation: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Lu
authors.affiliation: School of Urban and Environmental Science, Northwest University, Xi'an 710127, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States. Electronic address: thn@illinois.edu.
authors.forename: Thanh H
authors.initials: TH
authors.lastname: Nguyen
mesh_terms: D000260:Adenoviruses, Human / Q000737:chemistry* / Q000302:isolation & purification*; D000327:Adsorption; D045744:Cell Line, Tumor; D006801:Humans; D010316:Particle Size; D011145:Polyvinyls / Q000737:chemistry*; D013499:Surface Properties; D005465:Fluorocarbon Polymers
chemical_list: D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: Adenovirus; Extracellular polymeric substances; Membrane filtration; Random sequential adsorption