title: Genetic dissection of seed storability using two different populations with a same parent rice cultivar N22.
abstract: Seed storability in rice (Oryza sativa L.) is an important agronomic trait. Two segregating populations with N22 (indica) as a common parent, viz. a set of 122 backcross-inbred lines (BILs) derived from the backcross Nanjing35 (japonica)/N22//Nanjing35 and another population comprising 189 recombinant inbred lines (RILs) from the cross of USSR5 (japonica) and N22, were studied to detect quantitative trait loci (QTL) controlling seed storability. Germination percentage (GP) was used to evaluate seed storability after aging treated under three different conditions, viz. natural, artificial and combined aging treatments. A total of seven QTLs were identified on chromosomes 1, 2, 5, 6 and 9. Among them, a major QTL, qSSn-9, was common in the two populations. In contrast, four QTLs (qSSnj-2-1, qSSn-2-2, qSSn-5 and qSSn-6) were detected in BILs and the QTL qSSn-1 was identified in RILs, which was a new QTL for seed storability. The N22-derived alleles increased the seed storability at all the loci except qSSnj-2-1. We also investigated the effect of QTLs using five selected lines with high storability from BILs and verified qSSn-5 with a near-isogenic line (NIL). These results provide an opportunity for pyramiding or map-based cloning major QTLs for seed storability in rice.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Qiuyun
authors.initials: Q
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Yakun
authors.initials: Y
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Niqing
authors.initials: N
authors.lastname: He
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Ngo Thi
authors.initials: NT
authors.lastname: Hang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Linfang
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Linglong
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China ; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 , China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
keywords: artificial aging; backcross inbred lines (BILs); natural aging; quantitative trait locus (QTL); recombinant inbred lines (RILs); rice (Oryza sativa L.); seed storability