title: Analysis of informativeness of immunohistochemical and flow cytometric methods for estrogen receptor α assessment.
abstract: Informative capacity analysis of immunohistochemistry (IHC) and flow cytometry (FCM) in the assessment of estrogen receptor α (ERα) expression in breast cancer tissue was performed. Similar frequencies of expression were shown by both methods: 27% of ERα-negative and 73% ERα-positive cases. However, IHC evaluation detected low levels in only 20% of ERα-positive cases, whereas low levels of ERα detected by FCM were 2 times more often (48%). Moreover, FCM revealed positive expression (23-60%) in 33% of IHC ERα-negative cases. Among IHC ER-positive cases, zero ERα expression was detected by FCM in 12.5%. The approaches to minimize errors in routine clinical determination of the estrogen receptor status were proposed.
authors.affiliation: Blokhin Cancer Research Center, Russian Academy of Medical Science, Kashirskoe sh. 24, Moscow, 115478, Russia. bogush@orc.ru.
authors.forename: T A
authors.initials: TA
authors.lastname: Bogush
authors.affiliation: Blokhin Cancer Research Center, Russian Academy of Medical Science, Kashirskoe sh. 24, Moscow, 115478, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Dudko
authors.affiliation: Blokhin Cancer Research Center, Russian Academy of Medical Science, Kashirskoe sh. 24, Moscow, 115478, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Rodionova
authors.affiliation: Blokhin Cancer Research Center, Russian Academy of Medical Science, Kashirskoe sh. 24, Moscow, 115478, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Bogush
authors.affiliation: Blokhin Cancer Research Center, Russian Academy of Medical Science, Kashirskoe sh. 24, Moscow, 115478, Russia.
authors.forename: V J
authors.initials: VJ
authors.lastname: Kirsanov
authors.affiliation: Institute of Medicine, Ecology, and Physical Culture, Ulyanovsk State University, Ulyanovsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Rodionov
authors.affiliation: Blokhin Cancer Research Center, Russian Academy of Medical Science, Kashirskoe sh. 24, Moscow, 115478, Russia.
authors.forename: I K
authors.initials: IK
authors.lastname: Vorotnikov
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000175:diagnosis*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D014408:Biomarkers, Tumor; D047628:Estrogen Receptor alpha