title: [Identification of Bx02 Alleles and Genetic Analysis of the Involved Ancestry].
abstract: OBJECTIVE
  To identify the genotypes of the blood sample whose blood grouping showed discrepancies and study the ABO alleles' molecular characteristics of the involved ancestry.
  
  
  METHODS
  Blood samples were preliminary genotyped by PCR-SSP. Complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and cloning sequenced to identify its genotype.
  
  
  RESULTS
  Sequence analysis indicated that 3 samples of the family had an nt905A>G mutation in the B gene compared with ABO*B101. Combined with the serological results, the propositus could be typed as Bx02/O102.
  
  
  CONCLUSIONS
  DNA sequencing analysis is able to identify the serological phenotype samples that forward and reverse group methods were incongruous.
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Liu
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zheng
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Cao
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: He
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Xiang-Shu
authors.initials: XS
authors.lastname: Cheng
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China. E-mail: xinying_ji@henu.edu.cn.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Ji
mesh_terms: D000017:ABO Blood-Group System; D000483:Alleles; D001483:Base Sequence; D001788:Blood Grouping and Crossmatching; D005091:Exons; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D000017:ABO Blood-Group System