title: A Novel Production Method for High-Fructose Glucose Syrup from Sucrose-Containing Biomass by a Newly Isolated Strain of Osmotolerant Meyerozyma guilliermondii.
abstract: One osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. This selected strain, named SKENNY, was identified as Meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. SK-ENNY was utilized to produce high-fructose glucose syrup (HFGS) from sucrose-containing biomass. Conversion rates to HFGS from 310-610 g/l of pure sucrose and from 75-310 g/l of sugar beet molasses were 73.5-94.1% and 76.2-91.1%, respectively. In the syrups produced, fructose yields were 89.4-100% and 96.5-100% and glucose yields were 57.6-82.5% and 55.3-79.5% of the theoretical values for pure sucrose and molasses sugars, respectively. This is the first report of employing M. guilliermondii for production of HFGS from sucrose-containing biomass.
authors.affiliation: Faculty of Science, Al-Azhar University, Assiut Branch, Assiut, 71524, Egypt.|Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Sadat Mohammad Rezq
authors.initials: SM
authors.lastname: Khattab
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kodaki
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry; D018533:Biomass; D003470:Culture Media; D005285:Fermentation; D005516:Food Microbiology / Q000191:economics / Q000379:methods*; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D066248:High Fructose Corn Syrup; D008965:Molasses / Q000382:microbiology; D012335:RNA, Ribosomal; D013395:Sucrose / Q000032:analysis* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000502:physiology*
chemical_list: D003470:Culture Media; D066248:High Fructose Corn Syrup; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D005632:Fructose; D013395:Sucrose; D005947:Glucose
keywords: Fermentation; High-Fructose Glucose Syrup; Meyerozyma guilliermondii; Sucrose; Sugar Beet Molasses