title: Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis.
abstract: OBJECTIVE
  To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.
  
  
  METHODS
  Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).
  
  
  METHODS
  Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.
  
  
  RESULTS
  A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.
  
  
  CONCLUSIONS
  The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.
authors.affiliation: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark. bcmartel@NCSU.edu.|Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. bcmartel@NCSU.edu.|LEO Pharma A/S, Ballerup, Denmark. bcmartel@NCSU.edu.
authors.forename: Britta C
authors.initials: BC
authors.lastname: Martel
authors.affiliation: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Dyring-Andersen
authors.affiliation: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Lone
authors.initials: L
authors.lastname: Skov
authors.affiliation: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Kristian
authors.initials: K
authors.lastname: Thestrup-Pedersen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Skov
authors.affiliation: LEO Pharma A/S, Ballerup, Denmark.
authors.forename: Kresten
authors.initials: K
authors.lastname: Skak
authors.affiliation: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Lars K
authors.initials: LK
authors.lastname: Poulsen
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology*; D012867:Skin / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D016207:Cytokines
keywords: Atopic dermatitis; Cell culture; Cutaneous lymphocyte-associated antigen; Cytokine; Psoriasis; T cells