title: Dermatological evaluation of counter irritant effect of methanol leaf extract of Rumexvesicarius Linn. in rabbits.
abstract: OBJECTIVE
  To assess the counter-irritant and anti-inflammatory activity of Rumexvesicarius in dermatological use.
  
  
  METHODS
  The animal-based experimental study was conducted at the Royal Institute of Medical Sciences, Multan, Pakistan, in November 2014. Sodium lauryl sulfate, phenol, histamine and sandpaper irritation models were used. Irritation was induced by the clockwise frictional movement of fine sandpaper to the ear of rabbits and then applying sodium lauryl sulfate, histamine and phenol single topical application onto the ear of the rabbit. The counter-irritant effect was determined by calculating the mean decrease in redness and erythema with those of control and standard dexamethasone.
  
  
  RESULTS
  There were 20 rabbits in the study with a mean weight of 1.50±0.033 kg. R. vesicarius (100 and 150mg/mL) showed excellent counter-irritant effect when compared with control and standard groups. Both the doses depicted counter-irritant effect with the highest inhibition (94.42%) in sandpaper group, followed by sodium lauryl sulphate (90%), phenol (94.23%) and histamine (88.46%) irritation models respectively.
  
  
  CONCLUSIONS
  Methanol leaf extract of R. vesicarius countered the effect of irritation in experimental animals. It showed significant effect in terms of dose and counter-irritancy time.
authors.affiliation: Department of Physiology and Pharmacology, Royal Institute Medical Science, Multan, Pakistan.
authors.forename: Imran Ahmad
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Department of Physiology and Pharmacology, Royal Institute Medical Science, Multan, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Aziz
authors.affiliation: Department of Biochemistry, Royal Institute Medical Science, Multan, Pakistan.
authors.forename: Saira
authors.initials: S
authors.lastname: Bashir
authors.affiliation: Faculty of Veterinary Medicine, Higher College of Technology, Al Ain Men's College, UAE.
authors.forename: Muhammad Asif
authors.initials: MA
authors.lastname: Raza
authors.affiliation: Organic Plant Production & Agroecosystems Research in the Tropics and Subtropics, University of Kassel, Germany.
authors.forename: Ghayoor
authors.initials: G
authors.lastname: Fatima
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017453:Dermatitis, Irritant; D004195:Disease Models, Animal; D004202:Disinfectants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D017276:Friction; D006632:Histamine / Q000494:pharmacology; D017442:Histamine Agonists / Q000494:pharmacology; D000432:Methanol; D019800:Phenol / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011817:Rabbits; D031804:Rumex; D012867:Skin / Q000187:drug effects*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D004202:Disinfectants; D017442:Histamine Agonists; D010936:Plant Extracts; D013501:Surface-Active Agents; D019800:Phenol; D012967:Sodium Dodecyl Sulfate; D006632:Histamine; D000432:Methanol
keywords: Rumexvesicarius, Rabbit's skin, SLS, Counter-irritant, Dexamethasone histamine.