title: Increased micronucleus, nucleoplasmic bridge, nuclear bud frequency and oxidative DNA damage associated with prolactin levels and pituitary adenoma diameters in patients with prolactinoma.
abstract: Prolactinoma is the most common pituitary tumor. Most pituitary tumors are benign, but they often are clinically signiﬁcant. We investigated cytokinesis-block micronucleus cytome (CBMN cyt) assay parameters and oxidative DNA damage in patients with prolactinoma to assess the relations among age, prolactin level, pituitary adenoma diameter and 8-hydroxy-2'-deoxyguanosine (8-OHdG) level in patients with prolactinoma. We investigated 27 patients diagnosed with prolactinoma and 20 age- and sex-matched healthy controls. We measured CBMN cyt parameters and plasma 8-OHdG levels in peripheral blood lymphocytes of patients with prolactinoma and controls. The frequencies of micronucleus (MN), nucleoplasmic bridge, nuclear bud, apoptotic and necrotic cells, and plasma 8-OHdG levels in patients with prolactinoma were significantly greater than controls. MN frequency was correlated positively with age, prolactin levels and pituitary adenoma diameters in patients with prolactinoma. The increased chromosomal and oxidative DNA damage, and the positive correlation between MN frequency, prolactin levels and pituitary adenoma diameters may be associated with increased risk of cancer in patients with prolactinoma, because increased MN frequency is a predictor of cancer risk.
authors.affiliation: a Department of Chemical Technology , Technical Sciences Vocational School, Aksaray University , Aksaray , Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Bitgen
authors.affiliation: b Department of Medical Biology , Erciyes University , Kayseri , Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Donmez-Altuntas
authors.affiliation: c Department of Endocrinology and Metabolism , Erciyes University , Kayseri , Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Bayram
authors.affiliation: c Department of Endocrinology and Metabolism , Erciyes University , Kayseri , Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Cakir
authors.affiliation: b Department of Medical Biology , Erciyes University , Kayseri , Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Hamurcu
authors.affiliation: c Department of Endocrinology and Metabolism , Erciyes University , Kayseri , Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Diri
authors.affiliation: d Department of Biochemistry , Erciyes University , Kayseri , Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Baskol
authors.affiliation: e Department of Radiology , Faculty of Medicine, Erciyes University , Kayseri , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Senol
authors.affiliation: e Department of Radiology , Faculty of Medicine, Erciyes University , Kayseri , Turkey.
authors.forename: A C
authors.initials: AC
authors.lastname: Durak
mesh_terms: D000293:Adolescent; D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D048749:Cytokinesis / Q000502:physiology*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009336:Necrosis / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D015175:Prolactinoma / Q000378:metabolism*; D055815:Young Adult
chemical_list: D011388:Prolactin
keywords: 8-OHdG; CBMN cyt assay; apoptosis; cytokinesis-block micronucleus cytome; micronucleus; nuclear bud; nucleoplasmic bridge; prolactin; prolactinoma