title: The inhibitory effect of a Lactobacillus acidophilus derived biosurfactant on biofilm producer Serratia marcescens.
abstract: OBJECTIVE
  Serratia marcescens is one of the nosocomial pathogen with the ability to form biofilm which is an important feature in the pathogenesis of S. marcescens. The aim of this study was to determine the anti-adhesive properties of a biosurfactant isolated from Lactobacillus acidophilus ATCC 4356, on S. marcescens strains.
  
  
  METHODS
  Lactobacillus acidophilus ATCC 4356 was selected as a probiotic strain for biosurfactant production. Anti-adhesive activities was determined by pre-coating and co- incubating methods in 96-well culture plates.
  
  
  RESULTS
  The FTIR analysis of derived biosurfactant revealed the composition as protein component. Due to the release of such biosurfactants, L. acidophilus was able to interfere with the adhesion and biofilm formation of the S. marcescens strains. In co-incubation method, this biosurfactant in 2.5 mg/ml concentration showed anti-adhesive activity against all tested strains of S. marcescens (P<0.05).
  
  
  CONCLUSIONS
  Our results show that the anti-adhesive properties of L. acidophilus biosurfactant has the potential to be used against microorganisms responsible for infections in the urinary, vaginal and gastrointestinal tracts, as well as skin, making it a suitable alternative to conventional antibiotics.
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
authors.forename: Maliheh
authors.initials: M
authors.lastname: Shokouhfard
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
authors.forename: Rouha Kasra
authors.initials: RK
authors.lastname: Kermanshahi
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
authors.forename: Roya Vahedi
authors.initials: RV
authors.lastname: Shahandashti
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Feizabadi
authors.affiliation: Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Teimourian
keywords: Anti-adhesive; Biofilm formation; Biosurfactant; Co- incubation; Pre-coating