title: Dynamics of Fluoride Bioavailability in the Biofilms of Different Oral Surfaces after Amine Fluoride and Sodium Fluoride Application.
abstract: It was the aim of this study to investigate differences in fluoride bioavailability in different oral areas after the application of amine fluoride (AmF) and sodium fluoride (NaF). The null hypothesis suggested no differences in the fluoride bioavailability. The tongue coating was removed and biofilm samples from the palate, oral floor and cheeks were collected. All subjects brushed their teeth with toothpaste containing AmF or NaF. Specimens were collected before, as well as immediately after and at 30 and 120 minutes after tooth brushing. The fluoride concentration was determined. The area under the curve was calculated for each location and compared statistically. In the tongue coating, fluoride concentration increased faster after NaF application than after AmF application. After 30 minutes, the fluoride concentration decreased and remained stable until 120 minutes after AmF application and returned to baseline after NaF application. The difference between the baseline and the endpoint measurements was statistically significant. The fluoride concentration in the tongue coating remained at a higher level compared with the baseline for up to 120 minutes post-brushing. This may indicate that the tongue coating is a major reservoir for fluoride bioavailability. The results also indicate an unequal fluoride distribution in the oral cavity.
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Ella A
authors.initials: EA
authors.lastname: Naumova
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Dickten
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Rico
authors.initials: R
authors.lastname: Jung
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Krauss
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Henrik
authors.initials: H
authors.lastname: Rübesamen
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schmütsch
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Tudor
authors.initials: T
authors.lastname: Sandulescu
authors.affiliation: Dept. of Operative and Preventive Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Zimmer
authors.affiliation: Dept. of Biological and Material Sciences in Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany.
authors.forename: Wolfgang H
authors.initials: WH
authors.lastname: Arnold
mesh_terms: D000328:Adult; D018441:Biofilms / Q000187:drug effects*; D005260:Female; D005460:Fluorides, Topical / Q000494:pharmacology*; D006801:Humans; D008297:Male; D050296:Microbial Viability / Q000187:drug effects*; D009061:Mouth Mucosa / Q000382:microbiology*; D012463:Saliva / Q000382:microbiology; D012969:Sodium Fluoride / Q000494:pharmacology*; D013997:Time Factors; D014099:Toothbrushing; D055815:Young Adult
chemical_list: D005460:Fluorides, Topical; C094808:amine fluoride solution; D012969:Sodium Fluoride