title: Association of estrogen and progesterone receptor gene polymorphisms and their respective hormones in uterine leiomyomas.
abstract: The steroid hormones estradiol and progesterone play an important role in the pathophysiology of fibroids that occurs in 20-25 % of women in the reproductive age. Our study examines the risk imposed by estrogen and progesterone plasma levels in correlation with the ERβ (-13950T/C) and PGR (+331G/A) receptor gene polymorphisms. The study population included 296 individuals (146 UL cases and 150 female controls). Hormonal levels were estimated by ELISA and genotyping was carried out by PCR-RFLP analysis, and the obtained results were statistically analyzed. Estrogen levels were found to be high in cases with the "TC" genotype of ERβ receptor polymorphism compared to controls, whereas individuals with "GA" and "AA" genotype of PGR receptor polymorphism showed high progesterone levels for cases when compared to controls. The TC genotype of the ERβ receptor polymorphism and the GA and AA genotypes of the PGR receptor polymorphism and their respective hormonal levels can be developed as markers in the prediction of uterine fibroids.
authors.affiliation: Department of Genetics, University College of Science, Osmania University, Hyderabad, 500007, Telangana, India.
authors.forename: M
authors.initials: M
authors.lastname: Veronica
authors.affiliation: Department of Genetics, University College of Science, Osmania University, Hyderabad, 500007, Telangana, India.
authors.forename: Altaf
authors.initials: A
authors.lastname: Ali
authors.affiliation: Institute of Genetics and Hospital for Genetic Disorders, Ameerpet, Hyderabad, Telangana, India.
authors.forename: A
authors.initials: A
authors.lastname: Venkateshwari
authors.affiliation: Infertility Institute and Research Center, Secunderabad, Telangana, India.
authors.forename: D
authors.initials: D
authors.lastname: Mamata
authors.affiliation: Department of Genetics, University College of Science, Osmania University, Hyderabad, 500007, Telangana, India. prathinallari@yahoo.com.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Nallari
mesh_terms: D000328:Adult; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D047628:Estrogen Receptor alpha / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000235:genetics*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011374:Progesterone / Q000378:metabolism; D011379:Prognosis; D011980:Receptors, Progesterone / Q000235:genetics*; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D011980:Receptors, Progesterone; D011374:Progesterone
keywords: Estrogen; Infertility; Progesterone; Reproduction; Uterine leiomyomas