title: Limb remote ischemic postconditioning protects cerebral ischemia from injury associated with expression of HIF-1α in rats.
abstract: BACKGROUND
  Limb remote ischemic postconditioning (LRIP) can ameliorate cerebral ischemia-reperfusion injury (IRI), while the underlying mechanism remains elusive. Hypoxia-inducible factor 1α (HIF-1α) is an important transcription factor during cerebral ischemia damage. However, whether the neuroprotective effect of LRIP could be associated with HIF-1α is somewhat unclear. Here we tested the hypothesis that Limb remote ischemic postconditioning (LRIP) protecting brain from injury in middle cerebral artery occlusion (MCAO) rat model was associated with HIF-1α expression.
  
  
  RESULTS
  LRIP was conducted with 3 cycles of 10 min occlusion/10 min reperfusion at the beginning of reperfusion. The analysis of neurobehavioral function and triphenyltetrazolium chloride (TTC) staining showed the neurological deficit, brain infarct and cerebral edema, caused by ischemia-reperfusion injury (IRI), were dramatically ameliorated in LRIP administrated animals. Meanwhile, the result of Q-PCR and western blot revealed that the overexpression of HIF-1α induced by IRI could be notably suppressed by LRIP treatment.
  
  
  CONCLUSIONS
  LRIP exhibits a protective effect against cerebral ischemia/reperfusion and the possible mechanism is associated with the suppression of HIF-1α in stroke rats.
authors.affiliation: Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China. 18203228814@163.com.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zong
authors.affiliation: Department of Anesthesiology and Institute of Neurological Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan, 610041, China. vera15228287772@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China. 595112042@qq.com.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China. 841356070@qq.com.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology and Institute of Neurological Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan, 610041, China. 793219993@qq.com.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China. 734862297@qq.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China. he500209@163.com.
authors.forename: Huijun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology and Institute of Neurological Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan, 610041, China. 18190724110@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Anesthesiology and Institute of Neurological Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan, 610041, China. zyh24110@sina.com.
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. zhouxue7239@163.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China. woodwind@sohu.com.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China. neuron@yahoo.com.cn.|Department of Anesthesiology and Institute of Neurological Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan, 610041, China. neuron@yahoo.com.cn.
authors.forename: Tinghua
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001929:Brain Edema / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery; D057775:Ischemic Postconditioning / Q000379:methods*; D009043:Motor Activity; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012720:Severity of Illness Index; D020521:Stroke / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger