title: TPX2 promotes migration and invasion of human breast cancer cells.
abstract: OBJECTIVE
  To investigate the expression of targeting protein for Xenopus kinesin-like protein 2 (TPX2) in breast cancer tissue and to explore its role in proliferation, migration and invasion of breast cancer cells.
  
  
  METHODS
  The mRNA and protein expressions of TPX2 in breast cancer tissue and cell lines were assessed by quantitative RT-PCR and Western blot. The effect of TPX2 with RNA interference on proliferation, invasion and migration of breast cancer cells was observed by MTT and Transwell assays.
  
  
  RESULTS
  Both mRNA and protein expressions of TPX2 were upregulated in breast cancer tissues compared to tumor-adjacent tissue. TPX2 expression was also upregulated in breast cancer cell lines, and the TPX2 interfered by small interfering RNA could inhibit the proliferation, invasion and migration of breast cancer cells by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9.
  
  
  CONCLUSIONS
  Significantly upregulated TPX2 expression is observed in breast cancer tissue and cells, and contributes to promote the proliferation, migration and invasion of breast cancer cells.
authors.affiliation: Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Head and Neck Surgery, Thyroid and Neck Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300000, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Li
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan, Hubei 430000, China.
authors.forename: Na
authors.initials: N
authors.lastname: Shen
authors.affiliation: Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Yu
authors.affiliation: Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.
authors.forename: Jie-Bao
authors.initials: JB
authors.lastname: Li
authors.affiliation: Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.affiliation: Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China. Electronic address: zjh2396@163.com.
authors.forename: Jia-Heng
authors.initials: JH
authors.lastname: Zhang
keywords: Breast cancer; Invasion; Migration; Proliferation; TPX2