title: Overcoming Gene-Delivery Hurdles: Physiological Considerations for Nonviral Vectors.
abstract: With the use of contemporary tools and techniques, it has become possible to more precisely tune the biochemical mechanisms associated with using nonviral vectors for gene delivery. Consequently, nonviral vectors can incorporate numerous vector compositions and types of genetic cargo to develop diverse genetic therapies. Despite these advantages, gene-delivery strategies using nonviral vectors have poorly translated into clinical success due to preclinical experimental design considerations that inadequately predict therapeutic efficacy. Furthermore, the manufacturing and distribution processes are critical considerations for clinical application that should be considered when developing therapeutic platforms. In this review, we evaluate potential avenues towards improving the transition of gene-delivery technologies from in vitro assessment to human clinical therapy.
authors.affiliation: Abcombi Biosciences Inc, Buffalo, NY, USA; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Hill
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, BY, USA.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Fiber and Composite Materials, Feng Chia University, Taichung, Taiwan, ROC.
authors.forename: Chih-Kuang
authors.initials: CK
authors.lastname: Chen
authors.affiliation: Abcombi Biosciences Inc, Buffalo, NY, USA; Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, BY, USA. Electronic address: blainepf@buffalo.edu.
authors.forename: Blaine A
authors.initials: BA
authors.lastname: Pfeifer
authors.affiliation: Abcombi Biosciences Inc, Buffalo, NY, USA; Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, BY, USA. Electronic address: cjones9@buffalo.edu.
authors.forename: Charles H
authors.initials: CH
authors.lastname: Jones
mesh_terms: D016503:Drug Delivery Systems; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans