title: Effect of sustained release of rhBMP-2 from dried and wet hyaluronic acid hydrogel carriers compared with direct dip coating of rhBMP-2 on peri-implant osteogenesis of dental implants in canine mandibles.
abstract: Hyaluronic acid (HA) hydrogel has been used as a carrier of recombinant human bone morphogenetic protein (rhBMP)-2 for sustained delivery. To enhance peri-implant osteogenesis, a dried coating of rhBMP-2 HA hydrogel (BMP-HAH) on dental implants was designed; this approach provides the advantage of omitting in situ preparation of wet HA hydrogel. Sustained release of rhBMP-2 was more efficient for dried hydrogel over wet hydrogel. For both types, the released rhBMP-2 consistently led to enhanced alkaline phosphatase activity and osterix expression in human mesenchymal stromal cells. Histomorphometric analysis 4 weeks after placement of a dental implant in canine mandibles showed that the dried coating of BMP-HAH (10 μg/ml, n = 6) resulted in a significantly greater bone area (BA) than the wet BMP-HAH (10 μg/ml, n = 6) (p = 0.006) and implants without any coating (n = 6) (p = 0.022), while simple dip coating with rhBMP-2 (10 μg/ml, n = 6) resulted in significantly greater BA than the other three groups (p < 0.0005). Bone-to-implant contact (BIC) was significantly different only between the dried and wet coating of BMP-HAH (p = 0.014). Our results suggest that a simple dip coating of rhBMP-2 is more effective for increased peri-implant osteogenesis compared to a coating of BMP-HAH with sustained release.
authors.affiliation: Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chonnam National University Hospital, Republic of Korea; Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, BK21 Plus, Republic of Korea.
authors.forename: Jeong Joon
authors.initials: JJ
authors.lastname: Han
authors.affiliation: Department of Biomedical Engineering, Medical College, Korea University, Republic of Korea.
authors.forename: Yong-Doo
authors.initials: YD
authors.lastname: Park
authors.affiliation: Dental Research Institute, Seoul National University, Republic of Korea.
authors.forename: Tae Hyung
authors.initials: TH
authors.lastname: Cho
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, BK21 Plus, Republic of Korea; Dental Research Institute, Seoul National University, Republic of Korea. Electronic address: sjhwang@snu.ac.kr.
authors.forename: Soon Jung
authors.initials: SJ
authors.lastname: Hwang
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D003758:Dental Implantation, Endosseous; D004195:Disease Models, Animal; D004285:Dogs; D004337:Drug Carriers; D006801:Humans; D006820:Hyaluronic Acid; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008334:Mandible / Q000187:drug effects / Q000601:surgery*; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
chemical_list: D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid
keywords: Dip coating; Dried coating; Hyaluronic acid hydrogel; Peri-implant osteogenesis; rhBMP-2