title: Identification of lysine K18 acetylation on histone H3 peptide using gold nanoparticles' aggregation behaviour.
abstract: Acetylation of histones, the major protein component of eukaryotic chromosomes, contributes to the epigenetic regulation of gene expression and is also involved in cancer development. A recent study revealed the correlation between tumour formation and acetylation level of lysine K18 on histone H3. In this study, we developed two colorimetric in vitro assays using gold nanoparticles (AuNPs) for identification of lysine K18 acetylation on histone H3 peptide. In assay I, citrate ion-capped AuNP without further modification was employed. Simply mixing the K18 peptide with AuNP solution leads to distinct particle aggregation, relative to that by non-acetylated or lysine K14 acetylated control peptides. In assay II, an AuNP-peptide-antibody composite was synthesized and used as both the sensing probe and the transducing element. By mixing the sample peptides with the composite solution followed by PBS screening, different aggregation behaviours were observed between the K18 acetylated target peptide and the control sequences. Both assays are capable of identifying the acetylated peptides, and also differentiating the distinctive acetylation positions that differ merely by a distance of three amino acids.
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, #08-03, Innovis, Singapore, 138634, Singapore.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, #08-03, Innovis, Singapore, 138634, Singapore.
authors.forename: Laura
authors.initials: L
authors.lastname: Sutarlie
authors.affiliation: Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Centros, Singapore, 138668, Singapore.
authors.forename: Qiao Jing
authors.initials: QJ
authors.lastname: Lew
authors.affiliation: Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Centros, Singapore, 138668, Singapore.|Department of Microbiology, National University of Singapore, Block MD4, 5 Science Drive 2, Singapore, 117597, Singapore.
authors.forename: Sheng-Hao
authors.initials: SH
authors.lastname: Chao
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, #08-03, Innovis, Singapore, 138634, Singapore. xd-su@imre.a-star.edu.sg.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Su
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D003124:Colorimetry / Q000379:methods / Q000592:standards; D005427:Flocculation; D006046:Gold / Q000737:chemistry; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D000906:Antibodies; D002843:Chromatin; D006657:Histones; D010455:Peptides; D006046:Gold; D008239:Lysine
keywords: Colorimetric biosensing; Histone modification; Lysine acetylation; Peptide-functionalized gold nanoparticles