title: Development of a sensitive method for directly sequencing GII.4 norovirus genome.
abstract: A new strategy was proposed for amplifying and sequencing GII.4 norovirus genomes directly. A set of primer pairs was rationally designed, which amplified 6 overlapping fragments encompassing the whole genome. The sensitivity of new primers was comparable to that of detection primers, and 10 viral genome sequences were successfully obtained.
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xue
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Cai
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China. Electronic address: wuqp203@163.com.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Kou
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Guo
mesh_terms: D017250:Caliciviridae Infections / Q000175:diagnosis / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D029322:Norovirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000592:standards
keywords: GII.4; Genome; Norovirus; Phylogenetic analysis; Rational design; Sequencing