title: [Influence and mechanism of PinX1 gene on the chemotherapy sensitivity of nasopharyngeal carcinoma cells in response to Cisplatin].
abstract: OBJECTIVE
  To explore the influence and mechanism of PinX1 gene on the chemotherapy sensitivity of nasopharyngeal carcinoma cells in response to Cisplatin.
  
  
  METHODS
  Transfected nasopharyngeal carcinoma 5-8F cell lines with pCDH-CMV-PinX1-copGFP vector constructed by lentivirus to generate Lenti-PinX1-5-8F cells containing PinX1 gene, using Lenti-Ctrl-5-8F cell (blank vector without PinX1 gene was used to transfect 5-8F cell lines) and 5-8F cell as controls. Expression of PinX1 gene, telomerase activity, the inhibition of cancer cells proliferation, combined anticancer effect with Cisplatin and the expression of lung resistance protein (LRP) and Bcl-2 were detected with fluorescent quantitation polymerase chain reaction (PCR), flow cytometry, thiazolyl blue (MTT) method, areole test, Western blot and drug sensitivity test, respectively, in four groups (Lenti-PinX1-5-8F cell + Cisplatin, Lenti-PinX1-5-8F cell, Cisplatin and 5-8F cell) so as to explore the influence and mechanism of PinX1 gene on the chemotherapy sensitivity of nasopharyngeal carcinoma cells in response to Cisplatin.
  
  
  RESULTS
  The telomerase activity in Lenti-PinX1-5-8F cell (0.146 ± 0.004) was lower than those in the other two control cells (Lenti-Ctrl-5-8F cell: 0.967 ± 0.016, 5-8F cell: 1.000 ± 0.034, both P < 0.01). The cancer cell biological activity could be intensively inhibited by 16 µg/ml Cisplatin after lower level telomerase activity induced by PinX1 gene. Proliferation index (PI) (%) in Lenti-PinX1-5-8F cell + Cisplatin (14.39 ± 3.66) was also less than the other groups (Lenti-PinX1-5-8F cell, Cisplatin and 5-8F cell groups, 32.97 ± 3.00, 31.18 ± 4.24 and 47.19 ± 4.19, all P < 0.01). And same time, the expressions of LRP (0.64 ± 0.14) and Bcl-2 (0.57 ± 0.12) protein in Lenti-PinX1-5-8F cells were obviously reduced than those in other two group cells (Lenti-Ctrl-5-8F cell: 0.84 ± 0.19 and 0.81 ± 0.16; 5-8F cell: 0.83 ± 0.35 and 0.78 ± 0.27; all P < 0.01).
  
  
  CONCLUSIONS
  PinX1 gene can enhance the chemotherapy sensitivity of nasopharyngeal carcinoma cells in response to Cisplatin, which may be mediated by the down-regulation of telomerase activity and the inhibition of LRP and Bcl-2 gene in nasopharyngeal carcinoma cells.
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Congxiang
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China; Email: wenzhong60@163.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Keke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Guanxue
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Shenhua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Pearl River Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents; D002277:Carcinoma; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002945:Cisplatin; D015536:Down-Regulation; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms; D019098:Telomerase; D014162:Transfection; D025521:Tumor Suppressor Proteins
chemical_list: D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D025521:Tumor Suppressor Proteins; D019098:Telomerase; D002945:Cisplatin