title: Downregulation of Microrna-148a in Cancer-Associated Fibroblasts from Oral Cancer Promotes Cancer Cell Migration and Invasion by Targeting Wnt10b.
abstract: It is well established that crosstalk between cancer-associated fibroblasts (CAFs) and cancer cells plays a critical role in the occurrence and development of oral squamous cell carcinoma (OSCC). The molecular mechanisms underlying such interaction, however, remain far from clear. Accumulating data have indicated that microRNAs involved in tumor microenvironment, particularly in CAFs, contribute to the activation of fibroblasts and metastasis of cancer cells. Here, we showed that miR-148a was downregulated in CAFs compared with normal fibroblasts isolated from clinical OSCC tissue. Investigation of miR-148a function in fibroblasts demonstrated that overexpression of miR-148a in CAFs significantly impaired the migration and invasion of oral carcinoma cells (SCC-25) by directly targeting WNT10B. Taken together, these data suggested that miR-148a might be a novel candidate target for the treatment of OSCC.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People's Republic of China. minaj2012@outlook.com.
authors.forename: Anjie
authors.initials: A
authors.lastname: Min
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.|School of Stomatology, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Cancer Research Institute, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Peng
authors.affiliation: State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Cijun
authors.initials: C
authors.lastname: Shuai
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.|School of Stomatology, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.|School of Stomatology, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.|School of Stomatology, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Qian
authors.affiliation: The Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology, University of Aarhus, Aarhus C DK-8000, Denmark.|School of Stomatology, Tianjin Medical University, Tianjin, 300000, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Tong
authors.initials: T
authors.lastname: Su
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: C550570:MIRN148 microRNA, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; C491119:WNT10B protein, human; D051153:Wnt Proteins
keywords: Cancer-Associated Fibroblasts; MicroRNA-148a; Oral Squamous Cell Carcinoma; WNT10B