title: RNA polymerase I transcription is modulated by spatial learning in different brain regions.
abstract: Long-term memory is accompanied by changes in neuronal morphology and connectivity. These alterations are thought to depend upon new gene expression and protein synthesis over a distributed network of brain structures. Although much evidence supports the idea that the creation of stable, persistent memory traces requires synthesis of new proteins, the role of rRNA transcription and nucleolar activity in learning and memory has hardly been explored. rRNAs needed for protein synthesis result from the activity of two different RNA polymerases, RNA polymerase I and RNA polymerase III, transcribing for 47S RNA and 5S RNA, respectively. In this study, we first investigated the effects of spatial training in the Morris water maze on 47S RNA transcription in the central nervous system, demonstrating bidirectional modulation of its expression over a distributed neural network. We found learning-induced increases in the nucleolar organizer regions in the hippocampus. Finally, we demonstrated that intrahippocampal administrations of CX-5461 (0.6 μg/side), the specific RNA Polymerase I inhibitor, impair the ability of mice to locate the platform in the same task. These results suggest that de novo rRNA transcription is a necessary step for spatial memory consolidation, and that after learning, it occurs in several brain regions with a complex spatiotemporal dynamic. In this study, we demonstrate for the very first time that spatial learning modulates ribosomal RNA transcription in a wide brain circuit, with anatomical specificities in the dynamic of modulation. Together with pharmacological evidences, data presented here support the hypothesis of a necessary role of RNA Pol-I transcription during spatial memory formation. Read the Editorial Highlight for this article on page 673.
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Capitano
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gargiuli
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Angerilli
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.
authors.forename: Klizia
authors.initials: K
authors.lastname: Maccaroni
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Pelliccia
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.|Istituto Biologia Cellulare e Neurobiologia, CNR, Rome, Italy.|Centro di Ricerca in Neurobiologia "D. Bovet", Sapienza Università di Roma, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mele
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Rome, Italy.|Istituto di Biologia e Patologia Molecolari, CNR, Rome, Italy.|Istituto Pasteur, Fondazione Cenci Bolognetti, Rome, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Camilloni
keywords: learning; memory; nucleoli; ribosomal RNA