title: Effect of different biochars on antibiotic resistance genes and bacterial community during chicken manure composting.
abstract: Rice straw biochar (RSB) and mushroom biochar (MB) were added to lab-scale chicken manure composting to evaluate their effects on the behaviors of antibiotic resistance genes (ARGs) and on total and bio-available heavy metals (Cu, Zn and As). The associated bacterial community was characterized by 16SrRNA high-throughput sequencing. The abundance of pathogenic bacteria was also calculated. At the end of the control composting experiment, the average removal rate of ARGs was 0.86 log units and the removal rate of pathogenic bacteria was 57.1%. MB addition resulted in a higher removal rate than that in the control composting experiment. However, RSB addition yielded opposite results, which may be due to the higher abundance of Erysipelotrichaceae, Lactobacillaceae, Family_XI_Incertae_Sedis (belonging to Firmicutes carrying and disseminating ARGs) and pathogenic bacteria carrying ARGs. Furthermore, the correlations between bio-available heavy metals and ARGs were more obvious than those between total heavy metals and ARGs.
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Erping
authors.initials: E
authors.lastname: Cui
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Yiru
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: chen_hong@zju.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000363:Agaricales; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000472:pathogenicity; D002606:Charcoal / Q000494:pharmacology*; D002645:Chickens; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D008372:Manure / Q000382:microbiology*; D019216:Metals, Heavy / Q000032:analysis / Q000494:pharmacology; D059013:Microbial Consortia / Q000187:drug effects*; D012275:Oryza
chemical_list: D008372:Manure; D019216:Metals, Heavy; C540010:biochar; D002606:Charcoal
keywords: Antibiotic resistance gene; Bacterial community; Bio-available heavy metal; Biochar; Pathogenic bacteria