title: Synergistic Effect of Detection and Separation for Pathogen Using Magnetic Clusters.
abstract: Early diagnosis of infectious diseases is important for treatment; therefore, selective and rapid detection of pathogenic bacteria is essential for human health. We report a strategy for highly selective detection and rapid separation of pathogenic microorganisms using magnetic nanoparticle clusters. Our approach to develop probes for pathogenic bacteria, including Salmonella, is based on a theoretically optimized model for the size of clustered magnetic nanoparticles. The clusters were modified to provide enhanced aqueous solubility and versatile conjugation sites for antibody immobilization. The clusters with the desired magnetic property were then prepared at critical micelle concentration (CMC) by evaporation-induced self-assembly (EISA). Two different types of target-specific antibodies for H- and O-antigens were incorporated on the cluster surface for selective binding to biological compartments of the flagella and cell body, respectively. For the two different specific binding properties, Salmonella were effectively captured with the O-antibody-coated polysorbate 80-coated magnetic nanoclusters (PCMNCs). The synergistic effect of combining selective targeting and the clustered magnetic probe leads to both selective and rapid detection of infectious pathogens.
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Kook-Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Eun Sung
authors.initials: ES
authors.lastname: Kang
authors.forename: Geoncheol
authors.initials: G
authors.lastname: Jo
authors.forename: Se Young
authors.initials: SY
authors.lastname: Ahn
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Park
authors.affiliation: Life Science Division, Amogreentech Co. Ltd. , Seoul 137-902, Korea.
authors.forename: Sung Il
authors.initials: SI
authors.lastname: Kim
authors.affiliation: Life Science Division, Amogreentech Co. Ltd. , Seoul 137-902, Korea.
authors.forename: Saem
authors.initials: S
authors.lastname: Mun
authors.affiliation: Life Science Division, Amogreentech Co. Ltd. , Seoul 137-902, Korea.
authors.forename: Kyuwon
authors.initials: K
authors.lastname: Baek
authors.affiliation: Department of Chemistry and Research Institute for Natural Sciences, Korea University , Seoul 136-701, Korea.
authors.forename: Byeongyoon
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Research Institute for Natural Sciences, Korea University , Seoul 136-701, Korea.
authors.forename: Kwangyeol
authors.initials: K
authors.lastname: Lee
authors.forename: Wan Soo
authors.initials: WS
authors.lastname: Yun
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D065288:Serogroup; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000907:Antibodies, Bacterial; D011136:Polysorbates