title: Long noncoding RNA expression profiles of hypoxic pulmonary hypertension rat model.
abstract: OBJECTIVE
  Recently, long noncoding RNAs(lncRNAs) have been proved to be playing crucial roles in various biological processes, yet their role in hypoxic pulmonary hypertension (HPH) remains unclear. The aim of the present study is to investigate the lncRNA expression signatures in HPH rat model.
  
  
  METHODS
  Sprague Dawley rats were put in normobaric hypoxic chamber (FIO2 10%) eight hours a day or similar normoxic chamber for 3 weeks. Microarray was used to analyze the differential expression of lncRNAs and mRNAs between lung tissues of HPH rat model and control group. Dysregulated expression of selected lncRNAs was confirmed by Quantitative real-time PCR (qRT-PCR).
  
  
  RESULTS
  A total of 362 lncRNAs were identified to be significantly differentially expressed, among which 86 were up-regulated and 276 were down-regulated with fold-changes >/=2.0 between the two groups. The qRT-PCR results of lung tissues were in consistent with the microarray data.
  
  
  CONCLUSIONS
  The expression profile of lncRNAs was significantly altered in HPH rat model which may offer new insights into pathogenesis of this disease and could potentially provide novel diagnostic markers.
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.
authors.forename: Hua
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine. Electronic address: zrf2000@163.com.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Respiratory medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine. Electronic address: yingkejing@163.com.
authors.forename: Kejing
authors.initials: K
authors.lastname: Ying
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006801:Humans; D006976:Hypertension, Pulmonary / Q000235:genetics*; D008168:Lung / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D062085:RNA, Long Noncoding / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D062085:RNA, Long Noncoding
keywords: Expression profile; HPH rat model; Hypoxia; Hypoxic pulmonary hypertension; Long noncoding RNAs