title: Extract of Ettlia sp. YC001 Exerts Photoprotective Effects against UVB Irradiation in Normal Human Dermal Fibroblasts.
abstract: The identification of novel reagents that exert a biological ultraviolet (UV)-protective effect in skin cells represents an important strategy for preventing UV-induced skin aging. To this end, we investigated the potential protective effects of Ettlia sp. YC001 extracts against UV-induced cellular damage in normal human dermal fibroblasts (NHDFs). We generated four different extracts from Ettlia sp. YC001, and found that they exhibit low cytotoxicity in NHDFs. The ethyl acetate extract of Ettlia sp. YC001 markedly decreased UVB-induced cytotoxicity. Additionally, the ethyl acetate extract significantly inhibited the production of hydrogen peroxide-induced reactive oxygen species. Moreover, it inhibited UVB-induced thymine dimers, as confirmed by luciferase assay and thymine dimer dot-blot assay. Thus, the study findings suggest Ettlia sp. YC001 extract as a novel photoprotective reagent on UVB-induced cell dysfunctions in NHDFs.
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jeong-Ju
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Ki Bbeum
authors.initials: KB
authors.lastname: Kim
authors.affiliation: Sustainable Bioresource Research Center, KRIBB, Daejeon 34141, Republic of Korea.|Green Chemistry and Environmental Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
authors.forename: Jina
authors.initials: J
authors.lastname: Heo
authors.affiliation: Sustainable Bioresource Research Center, KRIBB, Daejeon 34141, Republic of Korea.
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Cho
authors.affiliation: Bioenergy and Biochemical Research Center, KRIBB, Daejeon 34141, Republic of Korea.|Green Chemistry and Environmental Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Sustainable Bioresource Research Center, KRIBB, Daejeon 34141, Republic of Korea.|Green Chemistry and Environmental Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
authors.forename: Hee-Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002338:Carotenoids / Q000494:pharmacology*; D002478:Cells, Cultured; D000460:Chlorophyta / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D020781:Matrix Metalloproteinase 1; D058086:Microalgae / Q000737:chemistry*; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D002338:Carotenoids; D006861:Hydrogen Peroxide; D020781:Matrix Metalloproteinase 1
keywords: Ettlia sp. YC001; dermal fibroblasts; photoaging; photoprotection; skin; ultraviolet B