title: Qualitation and Quantitation on Microplasma Jet for Bacteria Inactivation.
abstract: In this work, a self-made microplasma jet system was used to conduct the qualitation and quantitation of inactivation with Escherichia coli as the target bacteria. The logarithmic concentration and the size of antimicrobial rings served as the evaluation parameters, respectively. The effect of various parameters on inactivation effect was studied. The results showed that the majority of bacteria had been inactivated in 30 s. The inactivation effect enhanced and then weakened with the increase of air flow rate, and receded as the extension of treatment distance. The effect with different carrier gases showed as follows: oxygen > air > nitrogen > argon. Meanwhile, the effect of different components of microplasma was studied in the optimum conditions (The flow rate was 5 L/min; inactivation distance was 2 cm). The results showed that electrically neutral active species was the main factor of inactivation rather than heating effect, ultraviolet radiation and charged particles. Finally the experiments of thallus change proved that microplasma jet had etching effect on cell membrane. It also found that microplasma could degrade organic material like protein. Furthermore, the images of scanning electron microscope (SEM) revealed the change of cell morphology step by step in the whole process of inactivation.
authors.affiliation: Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: ChangMing
authors.initials: C
authors.lastname: Du
authors.affiliation: Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: YaNi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: ZiMing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Guangdong Experimental High School, GuangZhou, 510375, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Men
authors.affiliation: Guangdong Experimental High School, GuangZhou, 510375, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Men
authors.affiliation: Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000648:ultrastructure; D050296:Microbial Viability / Q000187:drug effects*; D058626:Plasma Gases / Q000494:pharmacology*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D058626:Plasma Gases