title: A corrosive oesophageal burn model in rats: Double-lumen central venous catheter usage.
abstract: BACKGROUND
  We aimed to create a new and less invasive experimental corrosive oesophageal burn model using a catheter without a gastric puncture (gastrotomy).
  
  
  METHODS
  We conducted the study with two groups composed of 8 male rats. The experimental oesophageal burn was established by the application of 10% sodium hydroxide to the distal oesophagus under a pressure of 20 cmH 2 O, via 5-F double-lumen central venous catheter without a gastrotomy. The control group was given 0.9% sodium chloride. All rats were killed 24 h after administration of NaOH or 0.9% NaCl. Histologic damage to oesophageal tissue was scored by a single pathologist blind to groups.
  
  
  RESULTS
  The rats in the control group were observed to have no pathological changes. Corrosive oesophagitis (tissue congestion, oedema, inflammation, ulcer and necrosis) was observed in rats exposed to NaOH.
  
  
  CONCLUSIONS
  We believe that an experimental corrosive oesophageal burn can safely be created under same hydrostatic pressure without a gastric puncture using this model.
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Turgut Ozal University, Ankara 06510, Turkey.
authors.forename: Vedat
authors.initials: V
authors.lastname: Bakan
authors.forename: Harun
authors.initials: H
authors.lastname: Ciralik
authors.forename: Seyfi
authors.initials: S
authors.lastname: Kartal
mesh_terms: D000818:Animals; D001706:Biopsy; D002057:Burns, Chemical / Q000150:complications / Q000175:diagnosis / Q000628:therapy*; D002424:Caustics / Q000633:toxicity; D062905:Central Venous Catheters; D004106:Dilatation / Q000295:instrumentation*; D004195:Disease Models, Animal; D004940:Esophageal Stenosis / Q000139:chemically induced / Q000175:diagnosis / Q000628:therapy*; D004945:Esophagoscopy; D004947:Esophagus / Q000000981:diagnostic imaging / Q000293:injuries*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002424:Caustics