title: Far-red fluorescent cell line for preclinical study of HER2-targeted agents.
abstract: We have created novel HER2-overexpressing human ovarian adenocarcinoma cell line stably transfected with far-red fluorescent protein TurboFP635. Growth characteristics, adhesion capacity and morphology of the cells do not differ from that of parental cell line. The obtained cell line (SKOV-kat) was confirmed to have high level of expression of the tumor marker HER2 comparable to that of initial cell line SKOV-3. The SKOV-kat cell line is designed for further establishment of experimental fluorescent models of HER2-overexpressing human ovarian cancer that make it possible to optimize and reduce the cost of preclinical study of agents for treatment of this disease.
authors.affiliation: Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia. irin-b@mail.ru.|Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. irin-b@mail.ru.
authors.forename: I V
authors.initials: IV
authors.lastname: Balalaeva
authors.affiliation: Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia.|Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Sokolova
authors.affiliation: Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia.|Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Brilkina
authors.affiliation: Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia.|Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
authors.forename: S M
authors.initials: SM
authors.lastname: Deyev
authors.affiliation: Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia.|Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
authors.forename: R V
authors.initials: RV
authors.lastname: Petrov
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D000097573:Red Fluorescent Protein; C508053:ERBB2 protein, human