title: The induction of metallothioneins during pulsed cadmium exposure to Daphnia magna: Recovery and trans-generational effect.
abstract: Although the importance of pulse exposure has gained ground in recent years, there were few studies on recovery and trans-generational effect of it. Two successive generations Daphnia magna were exposed to cadmium (Cd) pulses for 6h at the concentrations from 40 to 100 µg/l. The changes of tolerance and induction of MTs in exposed D. magna and their offspring were measured. The reduced tolerance of exposed D. magna was returned to levels similar to control after about 9 days in a generation. The level of MT still increased up to 3 days after exposure. In the experimental range, exposure duration played a decisive role in MT induction. The tolerance of F1 was lower than F0 and decreased with increasing pulsed concentrations of F0. Exposed to the same pulse, the MT levels of F1 were higher than the MT levels of F0, but the more obvious detoxification of MT in F1 had not been found. Our results suggest that pulsed cadmium exposure had impact on offspring of exposed organism and the risk assessment should take trans-generational effect into account.
authors.affiliation: School of Forestry, Beihua University, Jilin 132013, PR China. Electronic address: lis270@126.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130117, PR China.
authors.forename: Lianxi
authors.initials: L
authors.lastname: Sheng
authors.affiliation: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130117, PR China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Xu
authors.affiliation: Life Science Research Center, Beihua University, Jilin 132013, PR China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Tong
authors.affiliation: State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130117, PR China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology / Q000633:toxicity*; D003621:Daphnia / Q000187:drug effects*; D004361:Drug Tolerance; D004781:Environmental Exposure / Q000009:adverse effects*; D008668:Metallothionein / Q000378:metabolism*; D018570:Risk Assessment / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D008668:Metallothionein
keywords: Cd; Metallothioneins; Pulsed exposure; Recovery; Trans-generational effect