title: Physiological and biochemical changes of females of Piracanjuba, subjected to induced reproduction.
abstract: The aim of this experiment was to compare the level of fish female stress during induced reproduction with pituitary extract by two different methods, natural and semiextruded. The reproductive efficiency was 62.5% in the seminatural treatment and 100% in the extruded. Obtained egg volume was 5200 ml and 4000 ml, for seminatural and extruded treatments respectively. The mean number of eggs was 46.7 for the seminatural and 52.0 and for the extruded treatment. The percentage of viable eggs was, respectively, 87.2% and 8.17% for the natural treatment and extruded semimethods. Blood samples were collected to quantify cortisol and glucose levels, as well as red cell series and lymphocyte count. Fishes submitted to induction procedures showed elevated cortisol and glucose levels, compared to the control animals. The results for haematocrit, haemoglobin concentration and red blood cell count showed no significant differences among groups. Significant differences found in the number of lymphocytes and monocytes suggest the general adaptation syndrome. Our results suggest the reproductive induction process with extrusion of gametes as a more stressful method than seminatural reproduction process.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual do Norte do Paraná, Paraná, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Zanoni
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual do Norte do Paraná, Paraná, Brasil.
authors.forename: F G
authors.initials: FG
authors.lastname: Costa
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual do Norte do Paraná, Paraná, Brasil.
authors.forename: S
authors.initials: S
authors.lastname: de Carvalho
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual do Norte do Paraná, Paraná, Brasil.
authors.forename: F R F
authors.initials: FR
authors.lastname: Seiva
mesh_terms: D000818:Animals; D060125:Characiformes / Q000097:blood / Q000502:physiology*; D005260:Female; D010902:Pituitary Gland / Q000737:chemistry; D012098:Reproduction / Q000187:drug effects*; D014020:Tissue Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts
keywords: Brycon orbignyanus; blood glucose; cortisol; fish stress; haematology fish