title: The complete chloroplast genome of Tianshan Snow Lotus (Saussurea involucrata), a famous traditional Chinese medicinal plant of the family Asteraceae.
abstract: abtract We decoded the complete chloroplast DNA (cpDNA) sequence of the Tianshan Snow Lotus (Saussurea involucrata), a famous traditional Chinese medicinal plant of the family Asteraceae, by using next-generation sequencing technology. The genome consists of 152 490 bp containing a pair of inverted repeats (IRs) of 25 202 bp, which was separated by a large single-copy region and a small single-copy region of 83 446 bp and 18 639 bp, respectively. The genic regions account for 57.7% of whole cpDNA, and the GC content of the cpDNA was 37.7%. The S. involucrata cpDNA encodes 114 unigenes (82 protein-coding genes, 4 rRNA genes, and 28 tRNA genes). There are eight protein-coding genes (atpF, ndhA, ndhB, rpl2, rpoC1, rps16, clpP, and ycf3) and five tRNA genes (trnA-UGC, trnI-GAU, trnK-UUU, trnL-UAA, and trnV-UAC) containing introns. A phylogenetic analysis of the 11 complete cpDNA from Asteracease showed that S. involucrata is closely related to Centaurea diffusa (Diffuse Knapweed). The complete cpDNA of S. involucrata provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for Asteraceae.
authors.affiliation: a Research Room of Molecular Biology and Informatics , Xinjiang Normal University , Urumqi , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.affiliation: b Center of Excellence for the Oceans , National Taiwan Ocean University , Keelung , Taiwan.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: c Institute of Microbiology , Xinjiang Academy of Agricutural Science , Ulumuqi , China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Hao
authors.affiliation: d Faculty of Applied Sciences , Ton Duc Thang University , Ho Chi Minh City , Vietnam.|e Department of Bioscience Technology , Chung Yuan Christian University , Chung Li, Taiwan.
authors.forename: Phan Nhut
authors.initials: PN
authors.lastname: Nam
authors.affiliation: d Faculty of Applied Sciences , Ton Duc Thang University , Ho Chi Minh City , Vietnam.|e Department of Bioscience Technology , Chung Yuan Christian University , Chung Li, Taiwan.
authors.forename: Bui Thi
authors.initials: BT
authors.lastname: Ngoc Hieu
authors.affiliation: f WeThink Biotech Inc , Taoyuan , Taiwan.
authors.forename: Ching-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: a Research Room of Molecular Biology and Informatics , Xinjiang Normal University , Urumqi , China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhu
authors.affiliation: g Graduate Institute of Biotechnology , Chinese Culture University , Taipei , Taiwan.
authors.forename: Yen-Chang
authors.initials: YC
authors.lastname: Lin
authors.affiliation: e Department of Bioscience Technology , Chung Yuan Christian University , Chung Li, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
mesh_terms: D018742:DNA, Chloroplast / Q000235:genetics; D017343:Genes, Plant; D054628:Genome, Chloroplast; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D030441:Saussurea / Q000235:genetics*
chemical_list: D018742:DNA, Chloroplast; D010940:Plant Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Chloroplast genome; Saussurea involucrata; Snow lotus; next-generation sequencing