title: [Association of vitamin D receptor ApaI and TaqI gene polymorphisms with pigmented pretibial patches in T2DM patients].
abstract: OBJECTIVE
  To explore the association of vitamin D receptor ApaI and TaqI gene polymorphisms with pigmented pretibial patches (PPPs) in type 2 diabetes mellitus (T2DM) patients.
  
  
  METHODS
  A total of 233 patients with T2DM were enrolled. Patients were assigned to PPPs group (n=106) or non-PPPs (n=127) according to the presence of PPPs. Allelic and genotypic comparisons of VDR-TaqI and VDR-ApaI between the different groups were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The genotype and allele gene frequency were similar at ApaI in two groups (P>0.05). The PPPs group showed a higher frequency of TT allele at TaqI in the T2DM (95%) when compared to that of non-PPPs group (87%), while the frequency of Tt and tt allele were lower in PPPs group than non-PPPs group in T2DM (5% vs 13%, all P<0.05). The frequency of T allele were higher at TaqI in PPPs group (98%) than non-PPPs group (93%) in T2DM, while PPPs had a lower frequency of t allele (2% vs 7%, all P<0.05).
  
  
  CONCLUSIONS
  It seems that gene polymorphisms of VDR-TaqI, not VDR-ApaI, contributes to the risk of PPPs in T2DM patients in the Han nationality in Tianjin area.
authors.affiliation: Department of Endocrinology, Tianjin Medical University General Hospital, Tianjin 300052, China; Email: SWL13820515519@163.com.
authors.forename: Wenling
authors.initials: W
authors.lastname: Su
authors.affiliation: Department of Endocrinology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Mingcai
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Department of Endocrinology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.affiliation: Department of Endocrinology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Shaofang
authors.initials: S
authors.lastname: Tang
mesh_terms: D000483:Alleles; D003924:Diabetes Mellitus, Type 2; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol
chemical_list: D018167:Receptors, Calcitriol