title: Determining geographical variations in Ascaris suum isolated from different regions in northwest China through sequences of three mitochondrial genes.
abstract: The sequence diversities in three mitochondrial DNA (mtDNA) regions, namely portions of NADH dehydrogenase subunit 1 (pnad1), cytochrome c oxidase subunit 1 (pcox1), and NADH dehydrogenase subunit 4 (pnad4), were investigated in all Ascaris suum samples isolated from four regions in northwestern China. Those genes were amplified by PCR method and the lengths of pnad1, pcox1, and pnad4 were 419 bp, 711 bp, and 723 bp, respectively. The intraspecific sequence variations within A. suum samples were 0-2.9% for pnad1, 0-2.1% for pcox1, and 0-3.1% for pnad4. Phylogenetic analysis combined with three sequences of mtDNA fragments showed that all A. suum samples were monophyletic groups, but samples from the same geographical origin did not always cluster together. The results suggested that the three mtDNA fragments could not be used as molecular markers to identify the A. suum isolates from four regions, and have important implications for studying molecular epidemiology and population genetics of A. suum.
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China.
authors.forename: Ya-Xu
authors.initials: YX
authors.lastname: Guo
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China.
authors.forename: Hui-Bao
authors.initials: HB
authors.lastname: Wang
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China.
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Fang
authors.affiliation: b College of Agriculture and Animal Husbandry, Qinghai University , Xining , Qinghai Province , People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Kang
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000235:genetics*; D002681:China; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV / Q000235:genetics; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D058974:Phylogeography; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: Ascaris suum; genetic variability; mitochondrial gene; phylogenetic analysis