title: Conventional and enantioselective gas chromatography with microfabricated planar columns for analysis of real-world samples of plant volatile fraction.
abstract: Within a project exploring the application of lab-on-chip GC to in-field analysis of the plant volatile fraction, this study evaluated the performance of a set of planar columns (also known as microchannels, MEMS columns, or microfabricated columns) of different dimensions installed in a conventional GC unit. Circular double-spiral-shaped-channel planar columns with different square/rectangular sections up to 2m long were applied to the analysis of both essential oils and headspace samples of a group of medicinal and aromatic plants (chamomile, peppermint, sage, rosemary, lavender and bergamot) and of standard mixtures of related compounds; the results were compared to those obtained with reference narrow-bore columns (l:5m, dc:0.1mm, df:0.1 μm). The above essential oils and headspaces were first analyzed quali-and quantitatively with planar columns statically coated with conventional stationary phases (5%-phenyl-polymethylsiloxane and auto-bondable nitroterephthalic-acid-modified polyethylene glycol), and then submitted to chiral recognition of their diagnostic markers, by enantioselective GC with a planar columns coated with a cyclodextrin derivative (30% 6(I-VII)-O-TBDMS-3(I-VII)-O-ethyl-2(I-VII)-O-ethyl-β-cyclodextrin in PS-086). Column characteristics and analysis conditions were first optimized to obtain suitable retention and efficiency for the samples investigated. The planar columns tested showed performances close to the reference conventional narrow-bore columns, with theoretical plate numbers per meter (N/m) ranging from 6100 to 7200 for those coated with the conventional stationary phases, and above 5600 for those with the chiral selector.
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco (DSTF), via P. Giuria 9, Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cagliero
authors.affiliation: MEGA s.n.c.Via Plinio, 29, Legnano, Milano, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Galli
authors.affiliation: MEGA s.n.c.Via Plinio, 29, Legnano, Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Galli
authors.affiliation: CNR-IMM Bologna-Institute of Microelectronics and Microsystems, Via P. Gobetti, 101, Bologna, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Elmi
authors.affiliation: CNR-IMM Bologna-Institute of Microelectronics and Microsystems, Via P. Gobetti, 101, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Belluce
authors.affiliation: CNR-IMM Bologna-Institute of Microelectronics and Microsystems, Via P. Gobetti, 101, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Zampolli
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco (DSTF), via P. Giuria 9, Torino, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Sgorbini
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco (DSTF), via P. Giuria 9, Torino, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Rubiolo
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco (DSTF), via P. Giuria 9, Torino, Italy. Electronic address: carlo.bicchi@unito.it.
authors.forename: C
authors.initials: C
authors.lastname: Bicchi
mesh_terms: D002849:Chromatography, Gas / Q000295:instrumentation*; D003505:Cyclodextrins / Q000737:chemistry; D055616:Microtechnology; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D012828:Silicones; D047392:beta-Cyclodextrins; C078211:polymethylsiloxane; C068336:bergamot oil; C031215:betadex
keywords: Essential oils; Headspace sampling; Micro-GC; Planar columns; Plant volatile fraction