title: [Structure and function of histone chaperone FACT].
abstract: FACT is heterodimer protein complex and histone chaperone that plays an important role in maintaining and modifying chromatin structure during various DNA-dependent processes. FACT is involved in nucleosome assembly de novo and in the preservation and recovery of the nucleosome structure during and after transcription, replication and repair of DNA. During transcript elongation FACT reduces the height of the nucleosome barrier and supports survival of the nucleosomes during and after passage of RNA polymerase II. In this process FACT interacts with histone H2A-H2B dimer in nucleosomes, thus facilitating uncoiling of nucleosomal DNA from the octamer of histones; it also facilitates subsequent recovery of the canonical structure of the nucleosome after transcription. FACT also plays an important role in transformation of human cells and in maintaining the viability of the tumor cells.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, PA, 19111-2497 United States.|bond4@mail.ru.
authors.forename: M T
authors.initials: MT
authors.lastname: Bondarenko
authors.affiliation: Biology Department, Moscow State University, Moscow, 119991 Russia;
authors.forename: N V
authors.initials: NV
authors.lastname: Maluchenko
authors.affiliation: Biology Department, Moscow State University, Moscow, 119991 Russia;
authors.forename: M E
authors.initials: ME
authors.lastname: Valieva
authors.affiliation: Biology Department, Moscow State University, Moscow, 119991 Russia;
authors.forename: N S
authors.initials: NS
authors.lastname: Gerasimova
authors.affiliation: Fox Chase Cancer Center, Philadelphia, PA, 19111-2497 United States.|Biology Department, Moscow State University, Moscow, 119991 Russia;
authors.forename: O I
authors.initials: OI
authors.lastname: Kulaeva
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
authors.affiliation: Fox Chase Cancer Center, Philadelphia, PA, 19111-2497 United States.|Biology Department, Moscow State University, Moscow, 119991 Russia;|Vasily.Studitsky@fccc.edu.
authors.forename: V M
authors.initials: VM
authors.lastname: Studitsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D056488:Histone Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D056488:Histone Chaperones; D006657:Histones; D009707:Nucleosomes
keywords: FACT; chromatin; histones H2A-H2B; transcription