title: Dysregulation of oxygen hemodynamic responses to synaptic train stimulation in a rat hippocampal model of subarachnoid hemorrhage.
abstract: We investigated microvascular reactivity to synaptic train stimulation after induction of subarachnoid hemorrhage in adult rats, analyzing tissue oxygen levels [pO2] in intact hippocampus. In control rats, hippocampal pO2averaged 11.4 mm Hg whereas hemodynamic responses averaged 13.1 mm Hg (to a 25 s train). After subarachnoid hemorrhage (at 2 days), we recorded a dramatic elevation in baseline pO2in the hippocampus (to 68.4 mm Hg) accompanied by inverted pO2responses to synaptic train stimulation (-9.46 mm Hg). These significant changes in baseline hippocampal pO2and inverted pO2responses after subarachnoid hemorrhage indicate severe alterations of neurovascular coupling and neuronal viability.
authors.affiliation: Department of Neurosurgery (Surgery), Duke University Medical Center, Durham, NC, USA (Gavin Britz is currently at the Department of Neurosurgery, Houston Methodist Hospital, Houston, TX, USA) Research and Surgery Services, Durham VA Medical Center, Durham, NC, USA.
authors.forename: Francesca
authors.initials: F
authors.lastname: Galeffi
authors.affiliation: Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC, USA.
authors.forename: Simone
authors.initials: S
authors.lastname: Degan
authors.affiliation: Department of Neurosurgery (Surgery), Duke University Medical Center, Durham, NC, USA (Gavin Britz is currently at the Department of Neurosurgery, Houston Methodist Hospital, Houston, TX, USA).
authors.forename: Gavin
authors.initials: G
authors.lastname: Britz
authors.affiliation: Department of Neurosurgery (Surgery), Duke University Medical Center, Durham, NC, USA (Gavin Britz is currently at the Department of Neurosurgery, Houston Methodist Hospital, Houston, TX, USA) Research and Surgery Services, Durham VA Medical Center, Durham, NC, USA Department of Neurobiology, Duke University Medical Center, Durham, NC, USA dennis.turner@duke.edu.
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Turner
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D005260:Female; D006439:Hemodynamics; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006940:Hyperemia / Q000473:pathology; D008297:Male; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000378:metabolism* / Q000473:pathology; D013569:Synapses / Q000378:metabolism* / Q000473:pathology; D020301:Vasospasm, Intracranial / Q000378:metabolism / Q000473:pathology
keywords: Hippocampus; hemodynamics; hyperemia; neurovascular coupling; optical imaging; physiological stimulation; subarachnoid hemorrhage; tissue oxygen; vasospasm