title: PHARMACOLOGICAL REGULATION OF DIGESTION IN THE ANAUTOGENOUS FLESH FLY, Sarcophaga crassipalpis, BY SIMPLE INJECTION OF 6-HYDROXYDOPAMINE.
abstract: Female anautogenous Sarcophaga flesh flies need a protein meal to start large-scale yolk polypeptides (YPs) production and oocyte maturation. Protein meal rapidly elicits a brain-dependent increase in midgut proteolytic activity. Trypsin and chymotrypsin together represent over 80% of protease activity in liver-fed flies. Abdominal injection of 6-hydroxydopamine (6-OHDA) dose-dependently prohibits this increase in proteolytic activity at translational level in a similar way as post liver feeding decapitation. Delayed injection of 6-OHDA later than 6 h post liver meal has no effect. In flesh flies, chemical decapitation by 6-OHDA, by interrupting the brain-gut dopaminergic signaling, can be used as tool for the controlled inhibition of midgut proteolytic activity and subsequent ovarial development. Inhibition of ovarial development is probably indirect due to a deficit in circulating amino acids needed for YPs synthesis.
authors.affiliation: Research group of Insect Physiology and Molecular Ethology, KU Leuven, Leuven, Belgium.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bil
authors.affiliation: Research group of Insect Physiology and Molecular Ethology, KU Leuven, Leuven, Belgium.
authors.forename: Roger
authors.initials: R
authors.lastname: Huybrechts
mesh_terms: D000818:Animals; D004063:Digestion / Q000187:drug effects*; D005260:Female; D009490:Neurosecretory Systems / Q000187:drug effects; D009498:Neurotoxins / Q000494:pharmacology*; D016627:Oxidopamine / Q000494:pharmacology*; D058493:Sarcophagidae / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D009498:Neurotoxins; D016627:Oxidopamine
keywords: 6-hydroxydopamine; digestion; oxidopamine; vitellogenesis