title: [Recruiting of insulator protein ZIPIC of Drosophila melanogaster to minor binding sites in vivo depends on other DNA-binding transcription factors].
abstract: ZIPIC insulator protein of Drosophila has seven zinc finger domains at the C-terminus. Some of this zinc fingers are involved in binding of specific DNA sequence: CAGGGCTG. ZIPIC can interact only in vivo with minor form of this site (substitution of G to T at position 4). Possible explanation is interaction with additional transcription factors can help ZIPIC to bind minor form of consensus. On the other hand ZIPIC can efficiently bind in vitro other minor form of consensus (substitution of C to A at 6 position).
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|nickolay.zolotarev@yandex.ru.
authors.forename: N A
authors.initials: NA
authors.lastname: Zolotarev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Kyrchanova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors.forename: O G
authors.initials: OG
authors.lastname: Maksimenko
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
mesh_terms: D000818:Animals; D001665:Binding Sites; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D038101:Insulator Elements; D059372:Nucleotide Motifs; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D029721:Drosophila Proteins; D014157:Transcription Factors
keywords: C2H2 zinc fingers; ChIP-seq; DNA binding; EMSA; chromatin insulator; drosophila