title: Histological and immunohistochemical evaluation of stroma variations and their correlation with the Ki-67 index and expressions of glucose transporter 1 and monocarboxylate transporter 1 in canine thyroid C-cell carcinomas.
abstract: Canine thyroid C-cell carcinomas (CTCCs) are malignant tumors derived from calcitonin-producing C-cells of the thyroid gland. This study aimed to investigate the histological diversity of CTCCs from the viewpoint of stroma variations and to investigate their components by histological and immunohistochemical analyses including semiquantitative analysis of the density of microvessels (MVs) and α-SMA-positive cell count. Moreover, we examined whether the variations correlated with the Ki-67 index and expressions of glucose transporter 1 (GLUT-1) and monocarboxylate transporter 1 (MCT-1). Three stroma types (reticular, R, nest, N, and trabecular, T) were observed in CTCCs, and 21 cases were divided into 3 variations based on their combinations: mixed R and N (R/N) (n=7), simple N (n=7) and mixed T and N (T/N) (n=7). Immunohistochemically, stroma types depended on morphological features of α-SMA/fibronectin/laminin/collagen type IV-positive stroma cells. The density of MVs in R/N tended to be highest, and the density of those in N was significantly higher than the density of those in T/N (P=0.028). The α-SMA-positive cell count for N tended to be the lowest among the 3 variations. The Ki-67 index for R/N was significantly higher than those of the other variations (vs. N, P=0.007; vs. T/N, P=0.03), and that for T/N tended to be higher than that for N. Although there were no significant differences, GLUT-1 and MCT-1 expressions tended to be low in N. We concluded that stroma variations reflect tumor cell proliferation and expressions of GLUT-1 and MCT-1 in CTCCs.
authors.affiliation: Department of Veterinary Pathology, Rakuno Gakuen University, 582 Midori-machi, Bunkyo-dai, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Mizooku
authors.forename: Minoru
authors.initials: M
authors.lastname: Okamoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsuda
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Omachi
authors.forename: Kazuko
authors.initials: K
authors.lastname: Hirayama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kadosawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Taniyama
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D005260:Female; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism*; D012189:Retrospective Studies; D027981:Symporters / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D051272:Glucose Transporter Type 1; D019394:Ki-67 Antigen; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1