title: Construction and immune protection evaluation of recombinant polyvalent OmpAs derived from genetically divergent ompA by DNA shuffling.
abstract: A wide variety of bacterial infections is a major challenge in aquaculture. Development of polyvalent vaccines that can fight against as many pathogens as possible is especially necessary. The present study uses DNA shuffling to create a new hybrid OmpA with improved cross-protection against Vibrio alginolyticus and Edwardsiella tarda through the recombination of six OmpA genes from Vibrio parahaemolyticus, V. alginolyticus, E. tarda and Escherichia coli. Out of the 43 recombinant chimeras genes constructed using VA0764 primers, EompAs-19 was demonstrated as an ideal polyvalent vaccine against infections caused V. alginolyticus and E. tarda. Compared with VA0764, OmpAs-19 had three mutations, which may be a molecular basis of EompAs-19 as an efficient polyvalent vaccine against both V. alginolyticus and E. tarda infections. These results develop a polyvalent vaccine that prevents the infections caused by extracellular and intracellular bacteria. Thus, the present study highlights the way to develop polyvalent vaccines against microbial infections by DNA shuffling.
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China. Electronic address: lihui32@sysu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chu
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Zao-Hai
authors.initials: ZH
authors.lastname: Zeng
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China. Electronic address: pxuanx@sysu.edu.cn.
authors.forename: Xuan-Xian
authors.initials: XX
authors.lastname: Peng
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D032621:DNA Shuffling / Q000662:veterinary; D020609:Edwardsiella tarda / Q000235:genetics / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D044149:Vibrio alginolyticus / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000235:genetics; D015027:Zebrafish
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; C078167:OMPA outer membrane proteins
keywords: Aquaculture; DNA shuffling; Edwardsiella tarda; Polyvalent vaccine; Vibrio alginolyticus