title: Application of ionic liquids based enzyme-assisted extraction of chlorogenic acid from Eucommia ulmoides leaves.
abstract: A new approach for ionic liquid based enzyme-assisted extraction (ILEAE) of chlorogenic acid (CGA) from Eucommia ulmoides is presented in which enzyme pretreatment was used in ionic liquids aqueous media to enhance extraction yield. For this purpose, the solubility of CGA and the activity of cellulase were investigated in eight 1-alkyl-3-methylimidazolium ionic liquids. Cellulase in 0.5 M [C6mim]Br aqueous solution was found to provide better performance in extraction. The factors of ILEAE procedures including extraction time, extraction phase pH, extraction temperatures and enzyme concentrations were investigated. Moreover, the novel developed approach offered advantages in term of yield and efficiency compared with other conventional extraction techniques. Scanning electronic microscopy of plant samples indicated that cellulase treated cell wall in ionic liquid solution was subjected to extract, which led to more efficient extraction by reducing mass transfer barrier. The proposed ILEAE method would develope a continuous process for enzyme-assisted extraction including enzyme incubation and solvent extraction process. In this research, we propose a novel view for enzyme-assisted extraction of plant active component, besides concentrating on enzyme facilitated cell wall degradation, focusing on improvement of bad permeability of ionic liquids solutions.
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China. Electronic address: suixiaoyu@outlook.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sui
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Fu
mesh_terms: D002726:Chlorogenic Acid / Q000302:isolation & purification*; D004798:Enzymes / Q000737:chemistry*; D031283:Eucommiaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D004798:Enzymes; D052578:Ionic Liquids; D002726:Chlorogenic Acid
keywords: Cellulase; Chlorogenic acid; Eucommia ulmoides; Ionic liquid; Ionic liquid based enzyme-assisted extraction (ILEAE)