title: Determination of effective miRNAs in wound healing in an experimental Rat Model.
abstract: The larvae of Lucilia sericata have been used for centuries as medicinal maggots in the healing of wounds. The present study aimed to screen potential microRNAs related to ES-induced wound healing in rat skin wounds and to investigate the potential mechanisms contributing to accelerated wound healing. Healthy, male, 12 weeks old Wistar albino rats weighing 250-300 g were supplied by the Animal Experimental Center. All animal studies were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals. Wistar albino rats were treated by ES after post wounding and the differentially expressed miRNAs in wound biopsies were screened by microarray analysis at the end of treatments for 4,7 and 10 days. In addition, bioinformatics approaches were used to identify the potential target genes of differentially expressed miRNAs and the functions of their target genes. We found a significant up-regulation of rno-miR-99a* and rno-mir-877 in response to ES treatment. Further investigation of rno-miR-99a* and rno-mir-877 and their target genes (TGFa, TNF, TAGLN, MAPK1, MMP-9) implicated in present study could provide new insight for an understanding lead to the development of new treatment strategies. The identified miRNAs can be new biomarkers for ES- induced wound healing.
authors.affiliation: Istanbul University Department of Molecular Medicine, Institute of Experimental Medicine Istanbul Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Coskunpinar
authors.affiliation: Istanbul University Department of Medical Biology, Cerrahpasa Faculty of Medicine Istanbul Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Arkan
authors.affiliation: Ankara University Biotechnology Institute Ankara Turkey.
authors.forename: B G
authors.initials: BG
authors.lastname: Dedeoglu
authors.affiliation: Istanbul University Department of Medical Biology, Cerrahpasa Faculty of Medicine Istanbul Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Aksoz
authors.affiliation: Istanbul University Department of Microbiology and Clinical Microbiology, Cerrahpasa Faculty of Medicine Istanbul Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Polat
authors.affiliation: Istanbul University Department of Molecular Medicine, Institute of Experimental Medicine Istanbul Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Araz
authors.affiliation: Ankara University Biotechnology Institute Ankara Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Aydos
authors.affiliation: Ankara University Biotechnology Institute Ankara Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Oztemur
authors.affiliation: Bezmialem Vakif University Department of Medical Biology, Faculty of Medicine Istanbul Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Akbas
authors.affiliation: Istanbul University Department of Medical Biology, Cerrahpasa Faculty of Medicine Istanbul Turkey onaran.il@gmail.com.
authors.forename: I
authors.initials: I
authors.lastname: Onaran
mesh_terms: D000818:Animals; D012634:Bodily Secretions / Q000737:chemistry* / Q000378:metabolism; D000529:Complementary Therapies / Q000379:methods*; D019295:Computational Biology / Q000379:methods; D004175:Diptera / Q000737:chemistry / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007814:Larva / Q000737:chemistry / Q000502:physiology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics*; D014950:Wounds, Penetrating / Q000235:genetics / Q000473:pathology / Q000628:therapy*
chemical_list: D035683:MicroRNAs; D008840:Microfilament Proteins; D009124:Muscle Proteins; D016211:Transforming Growth Factor alpha; D014409:Tumor Necrosis Factor-alpha; C051615:transgelin; C535149:Mapk1 protein, rat; D019950:Mitogen-Activated Protein Kinase 1; D020780:Matrix Metalloproteinase 9