title: Intron or no intron: a matter for nuclear pore complexes.
abstract: Nuclear pore complexes (NPCs) have been shown to regulate distinct steps of the gene expression process, from transcription to mRNA export. In particular, mRNAs expressed from intron-containing genes are surveyed by a specific NPC-dependent quality control pathway ensuring that unspliced mRNAs are retained within the nucleus. In this Extra View, we summarize the different approaches that have been developed to evaluate the contribution of various NPC components to the expression of intron-containing genes. We further present the mechanistic models that could account for pre-mRNA retention at the nuclear side of NPCs. Finally, we discuss the possibility that other stages of intron-containing gene expression could be regulated by nuclear pores, in particular through the regulation of mRNA biogenesis factors by the NPC-associated SUMO protease Ulp1.
authors.affiliation: a Institut Jacques Monod; CNRS; UMR 7592; Univ Paris Diderot ; Sorbonne Paris Cité; Paris , France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Bonnet
authors.affiliation: a Institut Jacques Monod; CNRS; UMR 7592; Univ Paris Diderot ; Sorbonne Paris Cité; Paris , France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Palancade
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D007438:Introns; D008958:Models, Molecular; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D058207:Sumoylation; D014158:Transcription, Genetic
chemical_list: D028861:Nuclear Pore Complex Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D003546:Cysteine Endopeptidases; C118083:Ulp1 protease
keywords: SUMO-protease Ulp1; THO/TREX complex; mRNA export; mRNA quality control; mRNA transcription; nuclear pore complexes; sumoylation