title: Effect of low-concentration rhamnolipid on transport of Pseudomonas aeruginosa ATCC 9027 in an ideal porous medium with hydrophilic or hydrophobic surfaces.
abstract: The success of effective bioaugmentation processes for remediation of soil and groundwater contamination requires effective transport of the injected microorganisms in the subsurface environment. In this study, the effect of low concentrations of monorhamnolipid biosurfactant solutions on transport of Pseudomonas aeruginosa in an ideal porous medium (glass beads) with hydrophilic or hydrophobic surfaces was investigated by conducting miscible-displacement experiments. Transport behavior was examined for both glucose-grown and hexadecane-grown cells, with low and high surface hydrophobicity, respectively. A clean-bed colloid deposition model was used for determination of deposition rate coefficients. Results show that cells with high surface hydrophobicity exhibit greater retention than cells with low surface hydrophobicity. Rhamnolipid affects cell transport primarily by changing cell surface hydrophobicity, with an additional minor effect by increasing solution ionic strength. There is a good linear relation between k and rhamnolipid-regulated cell surface hydrophobicity presented as bacterial-adhesion-to-hydrocarbon (BATH) rate of cells (R(2)=0.71). The results of this study show the importance of hydrophobic interaction for transport of bacterial cells in silica-based porous media, and the potential of using low-concentration rhamnolipid solutions for facilitating bacterial transport in bioaugmentation efforts.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China; Department of Soil, Water and Environmental Science, University of Arizona, Tucson, AZ 85721, United States. Electronic address: zhonghua@email.arizona.edu.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address: 372462052@qq.com.
authors.forename: Guansheng
authors.initials: G
authors.lastname: Liu
authors.affiliation: The Sericultural Research Institute of Hunan Province, Changsha 410127, China. Electronic address: jiangyongbing000@126.com.
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Soil, Water and Environmental Science, University of Arizona, Tucson, AZ 85721, United States. Electronic address: brusseau@email.arizona.edu.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Brusseau
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address: lzf18182002@163.com.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address: liuyang_feiyang@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address: zgming@hnu.edu.cn.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
mesh_terms: D000473:Alkanes / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects; D001673:Biodegradation, Environmental; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D005898:Glass; D005947:Glucose / Q000378:metabolism; D006017:Glycolipids / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D009068:Movement / Q000187:drug effects; D009994:Osmolar Concentration; D016062:Porosity; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000473:Alkanes; D006017:Glycolipids; C418382:rhamnolipid; C007932:n-hexadecane; D005947:Glucose
keywords: Bacterial transport; Cell surface hydrophobicity; Deposition; Hydrophobic interaction; Rhamnolipid