title: Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose.
abstract: An anaerobic mesophile, Clostridium cellulovorans, produces a multienzyme complex called the cellulosome and actively degrades polysaccharides in the plant cell wall. C. cellulovorans also changes cellulosomal subunits to form highly active combinations dependent on the carbon substrate. A previous study reported on the synergistic effects of exoglucanase S (ExgS) and endoglucanase H (EngH) that are classified into the glycosyl hydrolase (GH) families 48, and 9, respectively. In this study, we investigated synergistic effects of ExgS and EngK, a GH9 cellulase different from EngH. In addition, since EngK was known to produce cellobiose as its main product, the inhibition on cellulase activity of EngK with cellobiose was examined. As a result, the effect of cellobiose inhibition on EngK coexistent with ExgS was found to be much lower than that with EngH. Thus, although EngH and EngK are in the same GH9 family, enzymatic activity in the presence of cellobiose was significantly different.
authors.affiliation: Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan. y-kosuke@bio.mie-u.ac.jp.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan.|Department of Bioinfomatics, Mie University Life Science Research Center, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan.|Laboratory of Applied Biotechnology, Mie University Industrial Technology Innovation Institute, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
keywords: Clostridium cellulovorans; Glycoside hydrolase family 48; Glycoside hydrolase family 9; Product inhibition; Synergistic effect