title: Amaurocine: Anti-Trichomonas vaginalis protein produced by the basidiomycete Amauroderma camerarium.
abstract: Trichomonas vaginalis is the causative agent of trichomoniasis, the most common nonviral STD worldwide. This infection can lead to severe health conditions, especially when women are affected. Metronidazole and tinidazole are the only choices of treatment. In this sense, natural bioactive compounds against T. vaginalis are an interesting approach in the search for more efficient therapies. Herein, amaurocine, a 12 kDa protein, produced by the mushroom Amauroderma camerarium was purified and tested against T. vaginalis, including two fresh clinical isolates. Amaurocine presented MIC values at 2.6 μM against the ATCC isolate 30236, and 5.2 μM against the fresh clinical isolates, TV-LACH1 and TV-LACM2. Furthermore, besides increasing human neutrophils nitric oxide release, amaurocine presented a low toxicity toward those cells, suggesting it exerts a proinflammatory character.
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Duarte
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, RS, Brazil; Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245, 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Seixas
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil; Chemical Microbiology, Helmholtz Center for Infection Research, Inhoffenstraße, 738124, Braunschweig, Germany.
authors.forename: Maira
authors.initials: M
authors.lastname: Peres de Carvalho
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil. Electronic address: tiana.tasca@ufrgs.br.
authors.forename: Tiana
authors.initials: T
authors.lastname: Tasca
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, RS, Brazil.
authors.forename: Alexandre José
authors.initials: AJ
authors.lastname: Macedo
mesh_terms: D000981:Antiprotozoal Agents / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020072:Polyporales / Q000737:chemistry / Q000378:metabolism*; D014246:Trichomonas vaginalis / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D005656:Fungal Proteins; C000607949:amaurocine protein, Amauroderma camerarium; D009569:Nitric Oxide
keywords: Amauroderma camerarium; Antiparasitic activity; Basidiomycota; Trichomonas vaginalis; Trichomoniasis