title: The influence of adiponectin on the transcriptomic profile of porcine luteal cells.
abstract: Reproductive functions are closely related to nutritional status. Recent studies suggest that adiponectin may be a hormonal link between them. Adiponectin is an adipocytokine, abundantly expressed in adipose tissues. It plays a dominant role in lipid and carbohydrate metabolism by stimulating fatty acid oxidation, decreasing plasma triglycerides, and increasing cells' sensitivity to insulin and has direct antiatherosclerotic effects. The hormone is also postulated to play a modulatory role in the regulation of the reproductive system. The aim of this study was to identify differentially expressed genes (DE-genes) in response to adiponectin treatment of porcine luteal ovarian cells. The global expression of genes in the porcine ovary was investigated using the Porcine (V2) Two-color gene expression microarray, 4 × 44 (Agilent, USA). Analysis of the microarray data showed that 701 genes were differentially expressed and 389 genes showed a fold change greater than 1.2 (p < 0.05). Among this number, 186 genes were up-regulated and 203 were down-regulated. The list of DE-genes was used for gene ontology analyses. The biological process list was generated from up-regulated and down-regulated DE-genes. We found that up-regulated products of DE-genes take part in 30 biological processes and down-regulated products in 9. Analysis of the interaction network among DE-genes showed that adiponectin interacts with genes involved in important processes in luteal cells. These results provide a basis for future work describing the detailed interactions and relationships explaining local regulation of adiponectin actions in the ovary of pigs.
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, ul.Oczapowskiego 1A, 10-719, Olsztyn, Kortowo, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Szeszko
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, ul.Oczapowskiego 1A, 10-719, Olsztyn, Kortowo, Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Smolinska
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, ul.Oczapowskiego 1A, 10-719, Olsztyn, Kortowo, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kiezun
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, ul.Oczapowskiego 1A, 10-719, Olsztyn, Kortowo, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Dobrzyn
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, ul.Oczapowskiego 1A, 10-719, Olsztyn, Kortowo, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Maleszka
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, ul.Oczapowskiego 1A, 10-719, Olsztyn, Kortowo, Poland. tkam@uwm.edu.pl.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kaminski
mesh_terms: D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D019295:Computational Biology / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D025941:Protein Interaction Mapping; D015398:Signal Transduction; D013552:Swine; D059467:Transcriptome
chemical_list: D052242:Adiponectin
keywords: Adiponectin; Differentially expressed genes; Porcine luteal cells