title: Mass spectrometric analysis of free fatty acids in infant milk powders by frozen pretreatment coupled with isotope-labeling derivatization.
abstract: In combination with frozen pretreatment and carboxyl group derivatization, a novel workflow was developed for the determination of free fatty acids in milk powder. The workflow showed a significantly enhanced performance for comprehensive free fatty acid analysis owing to a highly efficient frozen extraction method. In addition, the advantages of the workflow also involved high sensitivity and great tolerance to a complex matrix. Characteristic fragment ions of derivatization reagents also provide clear evidence for the qualitative analysis of free fatty acids. Fourteen types of free fatty acids in a number of domestic and overseas infant milk powders have been successfully detected. The content of free fatty acids in the different samples was different, which probably indicates the diverse quality of infant milk powder. The workflow is expected to be a pragmatic tool for the analysis of free fatty acids in intricate matrices.
authors.affiliation: Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai, PR China.
authors.forename: Tianxiao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Chemical Engineering, North University of China, Taiyuan, China.
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Leng
authors.affiliation: Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai, PR China.
authors.forename: Yaoshan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000737:chemistry*; D006801:Humans; D041943:Infant Formula / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011208:Powders / Q000737:chemistry
chemical_list: D005230:Fatty Acids, Nonesterified; D011208:Powders
keywords: Free fatty acids; Frozen pretreatment method; Infant milk powder; Mass spectrometry