title: p38 MAPK Participates in the Mediation of GLT-1 Up-regulation During the Induction of Brain Ischemic Tolerance by Cerebral Ischemic Preconditioning.
abstract: Our previous study has proved that the up-regulation of glial glutamate transporter 1 (GLT-1) played an important role in the acquisition of brain ischemic tolerance after cerebral ischemic preconditioning (CIP) in rats. However, little is known about the mechanism involved in the up-regulation of GLT-1 in the process. The present study investigates whether p38 MAPK, ERK1/2, and/or JNK participates in the up-regulation of GLT-1 during the induction of brain ischemic tolerance by CIP. It was found that CIP significantly enhanced the expression of p-p38 MAPK without altering p-ERK1/2 and p-JNK expression in the CA1 hippocampus. Inhibition of p38 MAPK function by its selective inhibitor SB203580 or knockdown p38 MAPK expression by its antisense oligodeoxynucleotides (AS-ODNs) suppressed the induction of brain ischemic tolerance. Furthermore, p38 MAPK was activated earlier than the up-regulation of GLT-1 in the CA1 hippocampus after CIP. Meanwhile, the expression of p-p38 MAPK by astrocytes was increased, and p38 MAPK AS-ODNs dose-dependently inhibited the up-regulation of GLT-1 after CIP. Taken together, it could be concluded that p38 MAPK participates in the mediation of GLT-1 up-regulation during the induction of brain ischemic tolerance after CIP.
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Jian-Xue
authors.initials: JX
authors.lastname: Gong
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Jia-Lei
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Meng-Yang
authors.initials: MY
authors.lastname: Jiang
authors.affiliation: Department of Science and Technology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang, 050000, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Yu-Yan
authors.initials: YY
authors.lastname: Hu
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xian
authors.affiliation: Department of Pathophysiology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People's Republic of China. liwbsjz@163.com.|Aging and Cognition Neuroscience Laboratory of Hebei Province, 89 Donggang Road, Shijiazhuang, 050031, People's Republic of China. liwbsjz@163.com.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D027342:Excitatory Amino Acid Transporter 2 / Q000096:biosynthesis*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D027342:Excitatory Amino Acid Transporter 2; C493395:Slc1a2 protein, rat; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Astrocyte; Cerebral ischemic preconditioning; GLT-1; Rat; p38 MAPK