title: Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity.
abstract: The use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide.
authors.affiliation: Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.|Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
authors.forename: Refaat B
authors.initials: RB
authors.lastname: Hamed
authors.affiliation: Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: J Ruben
authors.initials: JR
authors.lastname: Gomez-Castellanos
authors.affiliation: Structural Genomics Consortium, University of Oxford, Old Road Campus, Roosevelt Drive, Headington, OX3 7DQ, UK.
authors.forename: D
authors.initials: D
authors.lastname: Sean Froese
authors.affiliation: Structural Genomics Consortium, University of Oxford, Old Road Campus, Roosevelt Drive, Headington, OX3 7DQ, UK.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Krysztofinska
authors.affiliation: Structural Genomics Consortium, University of Oxford, Old Road Campus, Roosevelt Drive, Headington, OX3 7DQ, UK.
authors.forename: Wyatt W
authors.initials: WW
authors.lastname: Yue
authors.affiliation: Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK. christopher.schofield@chem.ox.ac.uk.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
mesh_terms: D004746:Enoyl-CoA Hydratase / Q000737:chemistry*; D019998:Racemases and Epimerases / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D004746:Enoyl-CoA Hydratase; D019998:Racemases and Epimerases; C021581:methylmalonyl-coenzyme A racemase
keywords: antibiotics; biosynthesis; carbapenem; crotonases; dynamic kinetic discrimination; enolate reactivity; stereoselectivity