title: Human dermal absorption of chlorinated organophosphate flame retardants; implications for human exposure.
abstract: Tris-2-chloroethyl phosphate (TCEP), tris (1-chloro-2-propyl) phosphate (TCIPP) and tris-1,3-dichloropropyl phosphate (TDCIPP) are organophosphate flame retardants (PFRs) widely applied in a plethora of consumer products despite their carcinogenic potential. Human dermal absorption of these PFRs is investigated for the first time using human ex vivo skin and EPISKIN™ models. Results of human ex vivo skin experiments revealed 28%, 25% and 13% absorption of the applied dose (500 ng/cm(2), finite dose) of TCEP, TCIPP and TDCIPP, respectively after 24h exposure. The EPISKIN™ model showed enhanced permeability values (i.e. weaker barrier), that were respectively 16%, 11% and 9% for TCEP, TCIPP and TDCIPP compared to human ex vivo skin. However, this difference was not significant (P>0.05). Estimated permeability constants (Kp, cm/h) showed a significant negative correlation with log Kow for the studied contaminants. The effect of hand-washing on dermal absorption of PFRs was investigated. Washing reduced overall dermal absorption, albeit to varying degrees depending on the physicochemical properties of the target PFRs. Moreover, slight variations of the absorbed dose were observed upon changing the dosing solution from acetone to 20% Tween 80 in water, indicating the potential influence of the dose vehicle on the dermal absorption of PFRs. Finally, estimated dermal uptake of the studied PFRs via contact with indoor dust was higher in UK toddlers (median ΣPFRs=36 ng/kg bw day) than adults (median ΣPFRs=4 ng/kg bw day). More research is required to fully elucidate the toxicological implications of such exposure.
authors.affiliation: Division of Environmental Health and Risk Management, School of Geography, Earth, and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom; Department of Analytical Chemistry, Faculty of Pharmacy, Assiut University, 71526 Assiut, Egypt. Electronic address: mae_abdallah@yahoo.co.uk.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abou-Elwafa Abdallah
authors.affiliation: Division of Environmental Health and Risk Management, School of Geography, Earth, and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom.
authors.forename: Gopal
authors.initials: G
authors.lastname: Pawar
authors.affiliation: Division of Environmental Health and Risk Management, School of Geography, Earth, and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom.
authors.forename: Stuart
authors.initials: S
authors.lastname: Harrad
mesh_terms: D000328:Adult; D002675:Child, Preschool; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005411:Flame Retardants / Q000378:metabolism* / Q000633:toxicity; D054879:Halogenation; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D010755:Organophosphates / Q000378:metabolism* / Q000633:toxicity; D012869:Skin Absorption / Q000187:drug effects* / Q000502:physiology*
chemical_list: D005411:Flame Retardants; D010755:Organophosphates
keywords: Dermal absorption; EPISKIN; Human exposure; Organophosphate flame retardants; PFRs