title: [Expression of CD131 Gene in Acute Myeloid Leukemic Cells].
abstract: OBJECTIVE
  To detect the expression level and the mutantion of CD131 in acute myeloid leukemic (AML) cells, and to analyze the relationship of CD131 expression level with clinical features.
  
  
  METHODS
  The peripheral blood mononuclear cells (PBMNC) from 44 AML patients and 25 healthy donors were collected, and the expression level of CD131 mRNA was detected by RT-PCR. The full length coding sequence of CD131 from 15 patients and 5 healthy donors was amplified by RT-PCR, and linked to pGEM-T vector to clone TA, 10 positive recombinant clones of each sample were analyzed by DNA sequencing. The AML patients were divided into CD131 negative and CD131 positive groups according the expression level of CD131, and white blood cell counts, CD34(+) cells percentage, complete remission rate of patients were compared.
  
  
  RESULTS
  CD131 was expressed at a lower level in AML than that in healthy donor. Five kinds of CD131 mutantions could be detected in both AML and healthy donor groups, but the mutation rate in AML (75.33%) was higher than that in healthy donors (18.0%). CD131 negative group showed a higher CD34(+) cells percentage (69.1% ± 20.8%), and lower complete remission rate (33.3%) than that in CD131 positive group (69.1% ± 20.8%, 33.3%). No statistically significant difference of WBC counts was found between 2 groups.
  
  
  CONCLUSIONS
  CD131 is expressed at a low level and shows high frequency of mutation in acute myeloid leukemic cells. CD131 negative AML correlats with high proportion of CD34(+) cells, and showed insensitive to chemotherapy.
authors.affiliation: Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Jing-Hui
authors.initials: JH
authors.lastname: Yang
authors.affiliation: Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Xian-Fang
authors.initials: XF
authors.lastname: Li
authors.affiliation: Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China. E-mail: chenyz@pub3.fz.fj.cn.
authors.forename: Yuan-Zhong
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D053648:Cytokine Receptor Common beta Subunit; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear; D009154:Mutation; D012333:RNA, Messenger; D012074:Remission Induction
chemical_list: C115876:CSF2RB protein, human; D053648:Cytokine Receptor Common beta Subunit; D012333:RNA, Messenger