title: Regulation of SCN3B/scn3b by Interleukin 2 (IL-2): IL-2 modulates SCN3B/scn3b transcript expression and increases sodium current in myocardial cells.
abstract: BACKGROUND
  In the initiation and maintenance of arrhythmia, inflammatory processes play an important role. IL-2 is a pro-inflammatory factor which is associated with the morbidity of arrhythmias, however, how IL-2 affects the cardiac electrophysiology is still unknown.
  
  
  METHODS
  In the present study, we observed the effect of IL-2 by qRT-PCR on the transcription of ion channel genes including SCN2A, SCN3A, SCN4A, SCN5A, SCN9A, SCN10A, SCN1B, SCN2B, SCN3B, KCNN1, KCNJ5, KCNE1, KCNE2, KCNE3, KCND3, KCNQ1, KCNA5, KCNH2 and CACNA1C. Western blot assays and electrophysiological studies were performed to demonstrate the effect of IL-2 on the translation of SCN3B/scn3b and sodium currents.
  
  
  RESULTS
  The results showed that transcriptional level of SCN3B was up-regulated significantly in Hela cells (3.28-fold, p = 0.022 compared with the control group). Consistent results were verified in HL-1 cells (3.73-fold, p = 0.012 compared with the control group). The result of electrophysiological studies showed that sodium current density increased significantly in cells which treated by IL-2 and the effect of IL-2 on sodium currents was independent of SCN3B (1.4 folds, p = 0.023). Western blot analysis showed IL-2 lead to the significantly increasing of p53 and scn3b (2.1 folds, p = 0.021 for p53; 3.1 folds, p = 0.023 for scn3b) in HL-1 cells. Consistent results were showed in HEK293 cells using qRT-PCR analysis (1.43 folds for P53, p = 0.022; 1.57 folds for SCN3B, p = 0.05).
  
  
  CONCLUSIONS
  The present study suggested that IL-2, may play role in the arrhythmia by regulating the expression of SCN3B and sodium current density.
authors.affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
authors.forename: Qiaobing
authors.initials: Q
authors.lastname: Sun
authors.affiliation: The Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Henan Research Institute of Population and Family Planning, National Health and Family Planning Commission Key Laboratory of Birth Defects Prevention, Zhengzhou, 450002, China.
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Mengchen
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Guangdong General Hospital, Guangdong, 510030, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Xue
authors.affiliation: The Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Cheng
authors.affiliation: First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan, 430074, China. xtu@hust.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Tu
mesh_terms: D015220:Calcium Channels / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000378:metabolism*; D062549:Voltage-Gated Sodium Channel beta-3 Subunit / Q000187:drug effects* / Q000235:genetics; D061566:Voltage-Gated Sodium Channels / Q000187:drug effects / Q000235:genetics; D020864:Calcium Channels, N-Type
chemical_list: D015220:Calcium Channels; D007376:Interleukin-2; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D012964:Sodium; D062549:Voltage-Gated Sodium Channel beta-3 Subunit; D061566:Voltage-Gated Sodium Channels; C105656:CACNA1A protein, human; D020864:Calcium Channels, N-Type; C493324:SCN3B protein, human