title: Identification and in silico characterisation of defective molecules associated with isolates of banana bunchy top virus.
abstract: Banana bunchy top virus (BBTV) is a multi-component single-stranded DNA virus. From 267 potentially infected Musa plants, 24 apparently 'defective' BBTV components have been identified. Interestingly, 23/24 of these defective molecules were apparently derived from DNA-R. All of the identified defective molecules had retained at least part of the CR-SL and CR-M but had insertions and/or deletions that in most cases resulted in open reading frame disruptions. Our detection of three monophyletic but diverse (and therefore likely circulating) defective DNA-R lineages suggests that, in many cases, defective DNA-R molecules might remain associated with BBTV genomes for prolonged periods.
authors.affiliation: School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
authors.forename: Daisy
authors.initials: D
authors.lastname: Stainton
authors.affiliation: Department of Clinical Laboratory Sciences, University of Cape Town, Cape Town, South Africa.
authors.forename: Darren P
authors.initials: DP
authors.lastname: Martin
authors.affiliation: School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
authors.forename: David A
authors.initials: DA
authors.lastname: Collings
authors.affiliation: Ecosciences Precinct, Queensland Alliance for Agriculture and Food Innovation, Centre for Plant Science, The University of Queensland, PO Box 46, Brisbane, QLD, 4001, Australia.
authors.forename: John E
authors.initials: JE
authors.lastname: Thomas
authors.affiliation: School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand. arvind.varsani@canterbury.ac.nz.|Department of Clinical Laboratory Sciences, University of Cape Town, Cape Town, South Africa. arvind.varsani@canterbury.ac.nz.|Department of Plant Pathology, Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, USA. arvind.varsani@canterbury.ac.nz.
authors.forename: Arvind
authors.identifier: http://orcid.org/0000-0003-4111-2415
authors.initials: A
authors.lastname: Varsani
mesh_terms: D052658:Babuvirus / Q000235:genetics*; D003198:Computer Simulation; D004279:DNA, Viral; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D009154:Mutation; D016366:Open Reading Frames; D010802:Phylogeny
chemical_list: D004279:DNA, Viral
keywords: Banana bunchy top virus; Defective molecules; Nanovirus