title: Development of Hf(4+)-immobilized polydopamine-coated magnetic graphene for highly selective enrichment of phosphopeptides.
abstract: In this work, we first designed and synthesized an IMAC material with Hf(4+) immobilized on polydopamine-coated magnetic graphene and applied it to phosphopeptides enrichment successfully. The newly prepared material gathered the advantages of large specific surface area of graphene, good hydrophilicity and biocompatibility of polydopamine and superparamagnetism of Fe3O4. We investigated the performance of Hf(4+)-immobilized polydopamine-coated magnetic graphene (denoted as magG@PDA-Hf(4+)) in phosphopeptides enrichment and the results showed high selectivity and sensitivity of the new material. Finally, we successfully applied magG@PDA-Hf(4+) to phosphopeptides enrichment from non-fat milk digests and human serum, further demonstrating excellent performance of this new material in phosphopeptides enrichment.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Haizhu
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006195:Hafnium / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D060328:Magnetic Phenomena; D008892:Milk / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002364:Caseins; D007211:Indoles; D010748:Phosphopeptides; D011108:Polymers; C568283:polydopamine; D012710:Serum Albumin, Bovine; D006108:Graphite; D006195:Hafnium
keywords: Hafnium; IMAC; MALDI; Phosphopeptides enrichment; Polydopamine