title: [Involvement of miR-199a in cisplatin resistance of ovarian cancer cell through modulating expression of mTOR].
abstract: OBJECTIVE
  To study the effect of miR-199a on the sensitivity of OV2008 and C13* cells to cisplatin and investigate its mechanism of action.
  
  
  METHODS
  Real-time polymerase chain reaction(PCR) and Western blot were applied to detect the expression level changes of mammalian target of rapamycin (mTOR) in OV2008 and C13* cells before and after transfecting the cells with the mimics and inhibitor of miR-199a with Lipofectamine 2000.We constructed luciferase reporter vectors of mTOR and then transfected OV2008 cells with the mimics of miR-199a with Lipofectamine 2000 to study the regulation of miR-199a's downstream target genes.
  
  
  RESULTS
  The expression level of mTOR was significantly higher in C13* than OV2008 cells (P<0.05). After OV2008 and C13* cells were transfected with the mimics and inhibitor of miR-199a, real-time PCR and Western blot showed that the mimics of miR-199a repressed the expression of mTOR; and the inhibitor of miR-199a promoted the expression of mTOR. After transfecting OV2008 cells with the mimics of miR-199a and luciferase reporter vectors of mTOR, renillaluciferase reporter gene analysis indicated the expression level of mTOR was negatively regulated by miR-199a.
  
  
  CONCLUSIONS
  miR-199a regulates the sensitivity of ovarian cancer cells to cisplatin through modulating expression of mTOR, and involves in the cisplatin resistance process of ovarian cancer cells.
authors.affiliation: Department of Gynecology, the First College of Clinical Medical Sciences & Yichang Central People's Hospital, China Three Gorges University, Yichang 443003, China.
authors.forename: Zhongxian
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Gynecology, the First College of Clinical Medical Sciences & Yichang Central People's Hospital, China Three Gorges University, Yichang 443003, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Gynecology, the First College of Clinical Medical Sciences & Yichang Central People's Hospital, China Three Gorges University, Yichang 443003, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Gynecology, the First College of Clinical Medical Sciences & Yichang Central People's Hospital, China Three Gorges University, Yichang 443003, China.
authors.forename: Youzhen
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Gynecology, the First College of Clinical Medical Sciences & Yichang Central People's Hospital, China Three Gorges University, Yichang 443003, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Gynecology, the First College of Clinical Medical Sciences & Yichang Central People's Hospital, China Three Gorges University, Yichang 443003, China; Email: tjmudoc@126.com.
authors.forename: Jianling
authors.initials: J
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D002945:Cisplatin; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases; D035683:MicroRNAs; D010051:Ovarian Neoplasms; D060888:Real-Time Polymerase Chain Reaction; D058570:TOR Serine-Threonine Kinases; D014162:Transfection
chemical_list: D035683:MicroRNAs; C531408:mirn199 microRNA, human; D008156:Luciferases; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D002945:Cisplatin