title: Seeding Homologous Adipose-Derived Stem Cells and Bladder Smooth Muscle Cells Into Bladder Submucosa Matrix for Reconstructing the Ureter in a Rabbit Model.
abstract: BACKGROUND
  Congenital or acquired abnormalities may result in ureteral malformation, trauma, or defect. Traditional reconstructive methods are often associated with numerous complications. Tissue engineering technology may provide an alternate avenue for ureteral reconstruction. In this study, we constructed tissue-engineered tubularized grafts (TETGs) by seeding homologous adipose-derived stem cells (ADSCs) and bladder smooth muscle cells (SMCs) into bladder submucosa matrix (BSM) for ureteral reconstruction in rabbit models.
  
  
  METHODS
  ADSCs and bladder SMCs were seeded onto 2 sides of the BSM, respectively. Then the grafts were used to construct TETGs of 4.0 cm length and 8.0 mm diameter and were transplanted into the omentum of rabbits for 2 weeks before ureteral reconstruction. The 4.0-cm segment of the ureter was replaced by the TETG. Evolutionary formation of tissue structures and degree of epithelization were evaluated with the use of histologic and immunohistochemical techniques at 2, 4, 8, and 16 weeks after implantation.
  
  
  RESULTS
  All of the rabbits were alive until they were killed. Histologic and immunohistochemical analyses showed consistent regeneration of mature and functional urothelium. At 16 weeks after TETG implantation, multilayered urothelium covered the entire lumen, with visible neovascularization in the center and formation of organized smooth muscle bundles.
  
  
  CONCLUSIONS
  We successfully constructed a tissue-engineered transplanted graft by seeding ADSCs and SMCs onto the BSM for ureteral repair and reconstruction in a rabbit model.
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.
authors.forename: L-C
authors.initials: LC
authors.lastname: Meng
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.
authors.forename: W-B
authors.initials: WB
authors.lastname: Liao
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China. Electronic address: sixingyang2002@163.com.
authors.forename: S-X
authors.initials: SX
authors.lastname: Yang
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Xiong
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.
authors.forename: C
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.
authors.forename: L-Q
authors.initials: LQ
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation; D000818:Animals; D004195:Disease Models, Animal; D032389:Myocytes, Smooth Muscle / Q000637:transplantation*; D011817:Rabbits; D062326:Re-Epithelialization; D019651:Plastic Surgery Procedures / Q000379:methods*; D033581:Stem Cell Transplantation / Q000379:methods*; D014513:Ureter / Q000601:surgery*; D001743:Urinary Bladder / Q000473:pathology / Q000601:surgery*; D013520:Urologic Surgical Procedures / Q000379:methods*