title: Characterization of six IL-17 family genes in miiuy croaker and evolution analysis of vertebrate IL-17 family.
abstract: Interleukin-17 (IL-17) family is a cytokine family which is one of the major signaling molecules family involved in immunity. Six member of IL-17 family cytokines (IL-17A-F) were found in mammals. In fish, all IL-17 family genes except IL-17B and IL-17E have been isolated and identified. Besides, IL-17N is uniquely found from teleosts. IL-17 family genes are widely studied in mammals, but have not been widely reported in lower vertebrates. In this study, we identify six IL-17 family genes (IL-17A/F1-3, IL-17C, IL-17D, IL-17N) from miiuy croaker, using LPS and poly (I:C) to infect miiuy croaker in order to analyze the expression response to bacteria and virus and expression in normal tissues. Challenge experiment showed that miiuy croaker IL-17 family genes exhibited more sensitive response to the poly (I:C) than the LPS. The expression of IL-17 in un-stimulated tissues showed that different gene has expressed in different tissues. Through the analysis of IL-17 family members exist in various representative species to study the evolution of the IL-17 family, and the result showed IL-17A/F, IL-17B, IL-17C, and IL-17D should be present in early gnathostomes species.
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China; School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, China.
authors.forename: Yuena
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, China.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Su
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, China.
authors.forename: Taiwu
authors.initials: T
authors.lastname: Li
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: tianjunxu@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D005810:Multigene Family; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D016415:Sequence Alignment
chemical_list: D029941:Fish Proteins; D020381:Interleukin-17; D008070:Lipopolysaccharides; D011070:Poly I-C
keywords: Cytokine; Expression; Interleukin; Miiuy croaker; Molecular evolution