title: Toad's egg-like cultivation process for forming microcarriers from nanofibrous hydrogel.
abstract: This paper proposes a new method for generating microcarries from bacterial cellulose (BC). BC, which is produced by specific bacteria, is a hydrogel composed of a three dimensional network structure formed by cellulose nanofibers. BC as an ECM-like nanofibrous material exhibits an excellent environment for cellular adhesion. Moreover, BC has a high biocompatibility and mechanical strength. From these properties, BC is expected to be applied for microcarriers, which is used for cultivating anchorage-dependent cells. Then, we developed a microfabrication process to create BC microcarriers by using gelatin microspheres as sacrificial architectures. In addition, the monodispersity of the formed BC microcarreirs was investigated.
authors.forename: K
authors.initials: K
authors.lastname: Higashi
authors.forename: N
authors.initials: N
authors.lastname: Miki
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000378:metabolism; D008863:Microspheres; D057139:Nanofibers / Q000737:chemistry*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin; D002482:Cellulose