title: [Differential expression profile of microRNAs between human bone marrow mesenchymal stem cells and sweat gland-like cells].
abstract: OBJECTIVE
  To explore the differential microRNAs (miRNAs) expression profiles between human bone marrow mesenchymal stem cells (hBM-MSCs) and sweat gland-like cells.
  
  
  METHODS
  The hBM-MSCs were induced to differentiate into osteogenic and adipogenic cells by related osteogenic differentiation basal medium and adipogenic differentiation basal medium respectively. And the results were assayed by oil red O and alizarin red stains respectively. When reaching 70%-80% confluence, primarily cultured sweat gland cells were heat-shocked and co-cultured with hBM-MSCs by a Transwell plate to induce the differentiation of hBM-MSCs. After 10 days, the total RNAs of hBM-MSCs and sweat gland-like cells were extracted, purified, labeled, hybridized and analyzed for predicting miRNAs target genes separately. The microarray results were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  There were 68 miRNAs of >double differentiation on expression level between hBM-MSCs and sweat gland-like cells. And 19 miRNAs were up-regulated and 49 miRNAs down-regulated. Moreover, there were 13 miRNAs with >5 folds of differential expression level between hBM-MSCs and sweat gland-like cells, including 5 up-regulated miRNAs (miRNA-132-3p, -4467, -4484, -146a-5p and -6126) and 8 down-regulated miRNAs (miRNA-708-5p, -138-5p, -6812-5p, -138-1-3p, -1281, -3157-3p, -4298 and -4459). There were 18 miRNAs related to regulation of the signaling pathways of sweat gland development, including nuclear factor-kappa B (NF-κB), mitogen-activated protein kinase/extracellular regulated protein kinase (MAPK/Erk), Wnt/beta-Catenin (Wnt/β-Catenin) and ectodysplasin-A1/ectodysplasin-A1 receptor (EDA/EDAR). The results of RT-PCR on miRNA-146a-5p and miRNA-6812-5p had a high concordance with the results of microarray.
  
  
  CONCLUSIONS
  There are obvious differentiation miRNAs expression profiles between hBM-MSCs and sweat gland-like cells. And 13 miRNAs >5 folds of differential expression and 18 miRNAs related to sweat gland development may play essential roles in regulated differentiation of hBM-MSCs into sweat gland-like cells.
authors.affiliation: Graduate School, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Tan
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhao
authors.forename: Huanjun
authors.initials: H
authors.lastname: Zhao
authors.forename: Along
authors.initials: A
authors.lastname: Zhao
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Zhao
authors.forename: Mengli
authors.initials: M
authors.lastname: Sun
authors.forename: Kui
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Research Laboratory of Wound Repair & Regeneration, First Affiliated Hospital, General Hospital of PLA, Beijing 100037, China, Email: zcp666666@sohu.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D015536:Down-Regulation; D006412:Hematopoietic Stem Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D035683:MicroRNAs; D020928:Mitogen-Activated Protein Kinases; D010012:Osteogenesis; D015398:Signal Transduction; D013545:Sweat Glands; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D035683:MicroRNAs; D051176:beta Catenin; D020928:Mitogen-Activated Protein Kinases