title: Diagnostic methods to cutaneous leishmaniasis detection in domestic dogs and cats.
abstract: Cutaneous leishmaniasis is caused by different species of Leishmania. In domestic animals such as dogs and cats, the diagnostic consists of clinical, epidemiological and serological tests, which changes among countries all around the world. Because of this diversity in the methods selected, we propose this systematic literature review to identify the methods of laboratory diagnosis used to detect cutaneous leishmaniasis in domestic dogs and cats in the Americas. Articles published in the last 5 years were searched in PubMed, ISI Web of Science, LILACS and Scielo, and we selected 10 papers about cutaneous leishmaniasis in dogs and cats in the Americas. In Brazil, often the indirect immunofluorescence and enzyme immunoassay (ELISA) have been applied. Other countries like United States and Mexico have been using antigenic fractions for antibodies detections by Western blot. ELISA and Western blot showed a higher sensitivity and efficacy in the detection of leishmaniasis. Analysis of sensibility and specificity of the methods was rarely used. Although confirmatory to leishmaniasis, direct methods for parasites detection and polymerase chain reaction showed low positivity in disease detection. We suggested that more than one method should be used for the detection of feline and canine leishmaniasis. Serological methods such as Western blot and enzyme immunoassay have a high efficacy in the diagnosis of this disease.
authors.affiliation: Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Daliah Alves Coelho
authors.initials: DA
authors.lastname: Trevisan
authors.affiliation: Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Maria Valdrinez Campana
authors.initials: MV
authors.lastname: Lonardoni
authors.affiliation: Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Izabel Galhardo
authors.initials: IG
authors.lastname: Demarchi
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity