title: Differential expression of minimal residual disease markers in peripheral blood and bone marrow samples from high-risk neuroblastoma patients.
abstract: Neuroblastoma is an aggressive solid tumor that leads to tumor relapse in more than half of high-risk patients. Minimal residual disease (MRD) is primarily responsible for tumor relapses and may be detected in peripheral blood (PB) and bone marrow (BM) samples. To evaluate the disease status and treatment response, a number of MRD detection protocols based on either common or distinct markers for PB and BM samples have been reported. However, the correlation between the expression of MRD markers in PB and BM samples remains elusive in the clinical samples. In the present study, the expression of 11 previously validated MRD markers (CHRNA3, CRMP1, DBH, DCX, DDC, GABRB3, GAP43, ISL1, KIF1A, PHOX2B and TH) was determined in 23 pairs of PB and BM samples collected from seven high-risk neuroblastoma patients at the same time point, and the sample was scored as MRD-positive if one of the MRD markers exceeded the normal range. Although the number of MRD-positive samples was not significantly different between PB and BM samples, the two most sensitive markers for PB samples (CRMP1 and KIF1A) were different from those for BM samples (PHOX2B and DBH). There was no statistically significant correlation between the expression of MRD markers in the PB and BM samples. These results suggest that MRD markers were differentially expressed in PB and BM samples from high-risk neuroblastoma patients.
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Hematology and Oncology, Kobe Children's Hospital, Kobe 654-0081, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Kozaki
authors.affiliation: Department of Epidemiology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Tri Budi
authors.initials: TB
authors.lastname: Hartomo
authors.affiliation: Department of Hematology and Oncology, Kobe Children's Hospital, Kobe 654-0081, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yanai
authors.affiliation: Department of Hematology and Oncology, Kobe Children's Hospital, Kobe 654-0081, Japan.
authors.forename: Daiichiro
authors.initials: D
authors.lastname: Hasegawa
authors.affiliation: Department of Hematology and Oncology, Kobe Children's Hospital, Kobe 654-0081, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kawasaki
authors.affiliation: Department of Hematology and Oncology, Kobe Children's Hospital, Kobe 654-0081, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kosaka
authors.affiliation: Department of Medical Rehabilitation, Kobe Gakuin University, Kobe 651-2180, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Matsuo
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirase
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hayakawa
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Kazumoto
authors.initials: K
authors.lastname: Iijima
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan ; Department of Epidemiology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Hisahide
authors.initials: H
authors.lastname: Nishio
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan ; Department of Epidemiology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nishimura
keywords: bone marrow; minimal residual disease; neuroblastoma; peripheral blood