title: Simultaneous quantification of cardiovascular disease related metabolic risk factors using liquid chromatography tandem mass spectrometry in human serum.
abstract: Recent observations from metabonomic studies have consistently found that branched-chain amino acids (BCAAs), aromatic amino acids (AAAs), glutamine (Gln), glutamic acid (Glu), Gln/Glu ratio, carnitine, and several species of acylcarnitines and lysophosphatidylcholines (LPCs) are possible risk factors for metabolic diseases such as diabetes mellitus (DM) and cardiovascular diseases (CVD). We described here a simple and reliable method for simultaneous quantification of these metabolic risk factors by liquid chromatography tandem mass spectrometry (LC-MS/MS). Serum samples were extracted with isopropanol, and the extracted metabolites were separated by hydrophilic interaction liquid chromatography (HILIC) and detected with electrospary ionization (ESI) inpositive ion mode with multiple reaction monitor (MRM) mode. All the metabolites were effectively separated within 5.5min. Analytical recoveries were in the range of 92.8-106.9%, with an average of 100.6%. The intra- run and total imprecisions for the measurement of these metabolites were 1.2-3.8% and 1.5-7.4%, respectively. Serum concentrations of the metabolites were analyzed in 123 apparently healthy volunteers. Significant associations between the metabolites and traditional CVD risk factors were observed. The newly developed LC-MS/MS method was simple, precise, and accurate and can be used as an efficient tool in CVD research and studies.
authors.affiliation: Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, 100005 Beijing, China; Beijing Hospital and National Center for Clinical Laboratories, 100730 Beijing, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China.
authors.forename: Ruiyue
authors.initials: R
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing Hospital and National Center for Clinical Laboratories, 100730 Beijing, China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Hospital and National Center for Clinical Laboratories, 100730 Beijing, China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Beijing Hospital and National Center for Clinical Laboratories, 100730 Beijing, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhao
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China.
authors.forename: Lijiao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Hospital and National Center for Clinical Laboratories, 100730 Beijing, China.
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, 100005 Beijing, China; The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, 100730 Beijing, China; Beijing Hospital and National Center for Clinical Laboratories, 100730 Beijing, China. Electronic address: wchen@bjhmoh.cn.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000097:blood* / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood* / Q000378:metabolism*; D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D012307:Risk Factors; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D000596:Amino Acids; C116917:acylcarnitine; D002331:Carnitine
keywords: Acylcarnitines; Amino acids; Cardiovascular disease; Carnitine; Liquid chromatography tandem mass spectrometry; Lysophosphatidylcholines