title: The Antibiosis Action and Rice-Induced Resistance, Mediated by a Lipopeptide from Bacillus amyloliquefaciens B014, in Controlling Rice Disease Caused by Xanthomonas oryzae pv. oryzae.
abstract: In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homologs. The minimum inhibition concentration and minimum bactericidal concentration of AXLP14 against Xoo were determined to be 1.25 and 2.50 mg/ml, respectively. At a concentration of 0.613 mg/ml, AXLP14 strongly inhibited the formation of Xoo biofilm. AXLP14 also inhibited the motility of Xoo in a concentration-dependent manner. Applying AXLP14 to rice seedlings significantly reduced the incidence and severity of disease caused by Xoo. In Xoo-infected rice seedlings, AXLP14 strongly and continuously up-regulated the expression of both OsNPR1 and OsWRKY45. In addition, AXLP14 effectively inhibited the Xoo-induced up-regulation of the expression of the abscisic acid biosynthesis gene OsNECD3 and the abscisic acid signalingresponsive gene OsLip9, indicating that AXLP14 may protect rice against Xoo-induced disease by enhancing salicylic acid defense and interfering with the abscisic acid response to virulence.
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, P.R. China.
authors.forename: Shu Bin
authors.initials: SB
authors.lastname: Li
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, P.R. China.
authors.forename: Shi Ru
authors.initials: SR
authors.lastname: Xu
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, P.R. China.
authors.forename: Rui Ning
authors.initials: RN
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, P.R. China.
authors.forename: Ren Chao
authors.initials: RC
authors.lastname: Zhou
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis / Q000235:genetics; D000898:Antibiosis; D000069977:Bacillus amyloliquefaciens / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D017343:Genes, Plant; D055666:Lipopeptides / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D012275:Oryza / Q000382:microbiology; D010456:Peptides, Cyclic / Q000302:isolation & purification; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology / Q000517:prevention & control*; D036226:Seedlings / Q000187:drug effects / Q000382:microbiology; D014974:Xanthomonas / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D003470:Culture Media; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide; D000040:Abscisic Acid
keywords: Antibiosis action; Bacillus lipopeptide; Induced resistance; Rice; Xanthomonas oryzae pv. oryzae