title: Measuring blood flow velocity from intravital video recordings.
abstract: There is an obvious scientific interest in computing blood flow velocity from intravital microscopy using digital video cameras attached to microscopes. Therefore, software capable of measuring blood flow velocity from videos is of major importance. In this work, a novel software tool is presented. The software tackles three main issues in velocity measurement from videos, the registration, segmentation, and finally the measuring itself. The software was tested in chick chorioallantoic membrane (CAM) videos captured with different resolutions, frame rates, and even cameras. The obtained results show the robustness achieved.
authors.forename: Pedro
authors.initials: P
authors.lastname: Guimarães
authors.forename: Weiwei
authors.initials: W
authors.lastname: Xiang
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Wigdahl
authors.forename: Bettina
authors.initials: B
authors.lastname: Reglin
authors.forename: Axel
authors.initials: A
authors.lastname: Pries
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ruggeri
mesh_terms: D000818:Animals; D016880:Anisotropy; D001783:Blood Flow Velocity; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D007091:Image Processing, Computer-Assisted; D012984:Software; D014741:Video Recording