title: Comparison of 2 chromogenic media for the detection of extended-spectrum β-lactamase producing Enterobacteriaceae stool carriage in nursing home residents.
abstract: ChromID ESBL agar and Brilliance ESBL agar were compared for the isolation of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from 298 stools. These had comparable sensitivity and selectivity for the 116 positive samples. Pre-enrichment with cefpodoxime and extending incubation to 48 hours after direct plating both significantly increased sensitivity but reduced selectivity of both agars.
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom. Electronic address: eb544@medschl.cam.ac.uk.
authors.forename: Beth
authors.initials: B
authors.lastname: Blane
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Hayley J
authors.initials: HJ
authors.lastname: Brodrick
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom; Public Health England, Clinical Microbiology and Public Health Laboratory, Cambridge, United Kingdom; Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom.
authors.forename: Theodore
authors.initials: T
authors.lastname: Gouliouris
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Kirsty E
authors.initials: KE
authors.lastname: Ambridge
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Angela D
authors.initials: AD
authors.lastname: Kidney
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Ludden
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand.
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom; Public Health England, Clinical Microbiology and Public Health Laboratory, Cambridge, United Kingdom; Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom.
authors.forename: M Estée
authors.initials: ME
authors.lastname: Török
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom; Public Health England, Clinical Microbiology and Public Health Laboratory, Cambridge, United Kingdom; Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom.
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002353:Carrier State; D015296:Ceftizoxime / Q000031:analogs & derivatives / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests / Q000379:methods; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D009735:Nursing Homes; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D000097623:Cefpodoxime
chemical_list: D000900:Anti-Bacterial Agents; D015296:Ceftizoxime; D001618:beta-Lactamases; D000097623:Cefpodoxime
keywords: Chromogenic agar; Detection; Enterobacteriaceae; Extended-spectrum β-lactamase; Selectivity; Sensitivity