title: Tissue factor is strongly expressed in pericarcinomatous tissue in patients with laryngeal carcinoma.
abstract: OBJECTIVE
  This study aimed to understand the relationship between tissue factor (TF) and laryngeal carcinoma.
  
  
  METHODS
  Differences in TF expression between pericarcinomatous and carcinomatous tissues were studied in patients with laryngeal carcinoma; the potential clinical significance of the observed differences is discussed. Immunohistochemical, western blot, and RT-PCR analyses were performed to assess the expression of TF at the protein and mRNA levels, and differences between pericarcinomatous and carcinomatous tissues in patients (n = 20) with laryngeal carcinoma were analyzed.
  
  
  RESULTS
  Expression of TF was significantly higher in pericarcinomatous tissues than in carcinomatous tissues (P < 0.01); furthermore, the intensity of TF mRNA expression was also significantly stronger in pericarcinomatous than in carcinomatous tissue (P < 0.001). Robust expression of TF was observed in pericarcinomatous tissues but not in carcinomatous tissues.
  
  
  CONCLUSIONS
  TF may contribute to the carcinogenesis and development of laryngeal carcinoma and may provide a marker for assessment of the degree of malignancy and the progression of laryngeal carcinoma. TF may also provide a new target for therapeutics for human head and neck cancer.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: The Fifth Hospital in Wuhan Wuhan 430000, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Hua
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Wuhan University School of Basic Medical Sciences Wuhan 430071, China ; Wuhan Hamilton Biotechnology Co. LTD Wuhan 430075, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Yao
authors.affiliation: Department of Biochemistry and Molecular Biology, Wuhan University School of Basic Medical Sciences Wuhan 430071, China ; Wuhan Hamilton Biotechnology Co. LTD Wuhan 430075, China.
authors.forename: Dongcheng
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Zezhang
authors.initials: Z
authors.lastname: Tao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D013925:Thromboplastin
keywords: Tissue factor; carcinomatous tissues; laryngeal carcinoma; pericarcinomatous tissues