title: Iron metabolism and oxidative profile of dogs naturally infected by Ehrlichia canis: Acute and subclinical disease.
abstract: The aim of this study was to evaluate the oxidant profile and iron metabolism in serum of dogs infected by Ehrlichia canis. Banked sera samples of dogs were divided into two groups: negative control (n = 17) and infected by E. canis on acute (n = 24), and subclinical (n = 18) phases of the disease. The eritrogram, leucogram, and platelet counts were evaluate as well as iron, ferritin, and transferrin levels, latent iron binding capacity (LIBC), and transferrin saturation index (TSI) concentration. In addition, the advanced oxidation protein products (AOPP) and ferric reducing ability of plasma (FRAP) in sera were also analyzed. Blood samples were examined for the presence of E. canis by PCR techniques. History and clinical signals were recorded for each dog. During the acute phase of the disease, infected animals showed thrombocytopenia and anemia when compared to healthy animals (P < 0.05) as a consequence of lower iron levels. Ferritin and transferrin levels were higher in both phases (acute and subclinical) of the disease. The AOPP and FRAP levels increased in infected animals on the acute phase; however, the opposite occurred in the subclinical phase. We concluded that dogs naturally infected by E. canis showed changes in the iron metabolism and developed an oxidant status in consequence of disease pathophysiology.
authors.affiliation: Department of Microbiology and Parasitology, Santa Maria Federal University (UFSM), Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil; Department of Veterinary Clinic and Surgery, Franca University (UNIFRAN), Franca, Brazil. Electronic address: crivellenti_lz@yahoo.com.br.
authors.forename: Leandro Z
authors.initials: LZ
authors.lastname: Crivellenti
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Sofia
authors.initials: S
authors.lastname: Borin-Crivellenti
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Jéssica R
authors.initials: JR
authors.lastname: Oliveira
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Stefanie B
authors.initials: SB
authors.lastname: Coelho
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Catarina M
authors.initials: CM
authors.lastname: Contin
authors.affiliation: Department of Clinical and Toxicological Analysis, Santa Maria Federal University (UFSM), Santa Maria, RS, Brazil.
authors.forename: Etiane
authors.initials: E
authors.lastname: Tatsch
authors.affiliation: Department of Clinical and Toxicological Analysis, Santa Maria Federal University (UFSM), Santa Maria, RS, Brazil.
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Aureo E
authors.initials: AE
authors.lastname: Santana
authors.affiliation: Department of Veterinary Medicine, Santa Catarina West University (UNOESC), Xanxerê, SC, Brazil.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Tonin
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Mirela
authors.initials: M
authors.lastname: Tinucci-Costa
authors.affiliation: Department of Animal Science, Santa Catarina State University (UDESC), Chapecó, SC, Brazil. Electronic address: dasilva.aleksandro@gmail.com.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000097:blood; D000818:Animals; D004285:Dogs; D041061:Ehrlichia canis; D016873:Ehrlichiosis / Q000662:veterinary*; D004909:Erythrocyte Indices; D007501:Iron / Q000378:metabolism*; D007958:Leukocyte Count; D010084:Oxidation-Reduction; D018384:Oxidative Stress
chemical_list: D062107:Advanced Oxidation Protein Products; D007501:Iron
keywords: Ehrlichia canis; Ferritin; Iron; Oxidants; Transferrin