title: Isothermal amplification detection of nucleic acids by a double-nicked beacon.
abstract: Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Shi
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Pan
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Zhong
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China. Electronic address: mcp169@163.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Ma
mesh_terms: D004277:DNA, Single-Stranded / Q000032:analysis*; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded
keywords: Cascade amplification; Double-nicked beacon; Isothermal amplification; Real-time fluorescence; ssDNA detection