title: Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test.
abstract: We observed increasing unserotypable (UT) Actinobacillus pleuropneumoniae isolates using agar gel diffusion (AGD) test. To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (UT-serovar 2) and 2 were serovar 15 (UT-serovar 15). We examined serotyping antigen extraction temperature to determine heat influence. UT-serovar 1 and 15 were influenced by heat, because their precipitation lines were observed in the case of low antigen extraction temperature. To investigate the relationship between antigenicity and genotype, we performed pulsed-field gel electrophoresis (PFGE) analysis using UT-serovar 2 and 15. The predominant PFGE pattern of UT-serovar 2 was identical to that of serovar 2.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Morioka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shimazaki
authors.forename: Mariko
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Shoko
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics / Q000276:immunology; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005779:Immunodiffusion / Q000662:veterinary*; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary; D012703:Serotyping / Q000379:methods / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology