title: [Immunomodulatory effect of Huangqi glycoprotein on mice with experimental autoimmune encephalomyelitis].
abstract: OBJECTIVE
  To explore the therapeutic effect and possible mechanism of Huangqi glycoprotein (HQGP) on the development of experimental autoimmune encephalomyelitis (EAE).
  
  
  METHODS
  Female C57BL/6 mice were immunized subcutaneously with myelin oligodendrocyte glycoprotein peptide-35-55 (MOG35-55) and divided into HQGP-treated group and EAE control group. Clinical score and body mass were recorded every other day. Inflammatory cell infiltrations of spinal cord were observed by HE and immunofluorescence staining. The cell viability of splenic mononuclear cells (MNCs) was detected by MTT assay. The release of NO was measured by Griess method. The levels of TNF-α, IL-6 and IFN-γ were determined by ELISA. The subtypes of CD4(+)T cells were analyzed by flow cytometry.
  
  
  RESULTS
  HQGP treatment delayed the onset of EAE, attenuated the clinical symptoms and inhibited CD68(+) macrophage infiltration into the central nervous system. HQGP inhibited the viability of splenic MNCs, downregulated the secretion of NO, TNF-α, IL-6, and increased the secretion of IFN-γ. In addition, HQGP treatment effectively increased the numbers of CD4(+)CD25(+) T cells, CD4(+)IL-10(+) T cells and CD4(+)IFN-γ(+) T cells.
  
  
  CONCLUSIONS
  HQGP relieve the inflammation of EAE via reducing the number of T cell subsets and inhibiting the secretion of inflammatory cytokines.
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Peijun
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Minfang
authors.initials: M
authors.lastname: Guo
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Debing
authors.initials: D
authors.lastname: Yang
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: "2011" Collaborative Innovation Center/Research Center of Neurobiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030619, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Xue
authors.affiliation: Institute of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009; "2011" Collaborative Innovation Center/Research Center of Neurobiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030619, China. *Corresponding author, E-mail: macungen2001@163.com.
authors.forename: Cungen
authors.initials: C
authors.lastname: Ma
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000378:metabolism; D027885:Astragalus propinquus / Q000737:chemistry; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D006023:Glycoproteins; D007155:Immunologic Factors; D063308:Myelin-Oligodendrocyte Glycoprotein; D009569:Nitric Oxide; C067980:CD68 antigen, human; D000097382:CD68 Molecule