title: Study of Trichostation A-Induced Expression of Costimulatory Molecules CD80 and CD86 in Acute Myelocytic Leukemia Cells.
abstract: OBJECTIVE
  To investigate the trichostain A (TSA)-induced expression of costinmulatory molecules CD80 and CD86 in HL-60, K562 and mononuclear cells (MNC) of bone marrow in AML patients and its clinical significance.
  
  
  METHODS
  The TSA-induced expression of costimulatory molecules CD80, CD86 in HL-60, K562 and BMMNC, and the cell viability were detected by flow cytometry; the mRNA expression of CD80 and CD86 was detected by RT-PCR; after the TSA-induced HL-60 cells and K562 cells were irradiated with 75 Gy, the effect of these cells on proliferation of PBMNC from healthy volunteers was determined with CCK-8 method.
  
  
  RESULTS
  The HL-60 cells and BMMNC in AML patients expressed CD86, not expressed CD80, while the K562 cells not expressed CD86 and CD80. TSA could up-regulate the expression of CD86 in HL-60 cells and BMMNC of AML patients. The TSA-induced HL-60 cells expressing costimulatory molecule CD86 showed the proliferative effect on BMMNC from healthy volunteers.
  
  
  CONCLUSIONS
  The TSA can induce the expression of costimulatory molecule CD86 in HL-60 cells and BMMNC in AML patients, and can improve the proliferation of PBMNC in healthy volunteers.
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: Mei-Xia
authors.initials: MX
authors.lastname: Yu
authors.affiliation: Division of Nephrology, Department of Internal Medicine, The Third Affiliated Hospital of SUN Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: You-Fa
authors.initials: YF
authors.lastname: Chen
authors.affiliation: Department of Biopharmacy, School of Pharmacy, Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Central Laboratory, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: Dong-Xia
authors.initials: DX
authors.lastname: Hu
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Shen
authors.affiliation: Department of Oncology Clinical Pharmacy, Brigham and Women's Hospital, Harvard Medical School, Boston, USA, MA 02115. E-mail: Jni2@partners.org.
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China. E-mail: zhym112@126.com.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhou
mesh_terms: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D045744:Cell Line, Tumor; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D006877:Hydroxamic Acids; D015470:Leukemia, Myeloid, Acute
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D006877:Hydroxamic Acids; C012589:trichostatin A