title: The Structure-Activity Relationship between Marine Algae Polysaccharides and Anti-Complement Activity.
abstract: In this study, 33 different polysaccharides were prepared to investigate the structure-activity relationships between the polysaccharides, mainly from marine algae, and anti-complement activity in the classical pathway. Factors considered included extraction methods, fractionations, molecular weight, molar ratio of galactose to fucose, sulfate, uronic acid (UA) content, linkage, branching, and the type of monosaccharide. It was shown that the larger the molecular weights, the better the activities. The molar ratio of galactose (Gal) to fucose (Fuc) was a positive factor at a concentration lower than 10 µg/mL, while it had no effect at a concentration more than 10 µg/mL. In addition, sulfate was necessary; however, the sulfate content, the sulfate pattern, linkage and branching had no effect at a concentration of more than 10 µg/mL. Moreover, the type of monosaccharide had no effect. Laminaran and UA fractions had no activity; however, they could reduce the activity by decreasing the effective concentration of the active composition when they were mixed with the active compositions. The effect of the extraction methods could not be determined. Finally, it was observed that sulfated galactofucan showed good anti-complement activity after separation.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. jinweihua@qdio.ac.cn.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. jinweihua@qdio.ac.cn.
authors.forename: Weihua
authors.initials: W
authors.lastname: Jin
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wenjingwing@126.com.|College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, China. wenjingwing@126.com.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The School of Materials Sciences and Chemical Engineering, Ningbo University, Ningbo 315211, China. lianghongze@nbu.edu.cn.
authors.forename: Hongze
authors.initials: H
authors.lastname: Liang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. qbzhang@qdio.ac.cn.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. qbzhang@qdio.ac.cn.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology; D003167:Complement Activation / Q000187:drug effects; D006801:Humans; D000459:Phaeophyceae; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides
keywords: anti-complement activity; marine algae polysaccharides; structure-activity relationship