title: Simultaneous RNA-DNA FISH.
abstract: A highly useful tool for studying lncRNAs is simultaneous RNA-DNA FISH, which reveals the localization and quantitative information of RNA and DNA in cellular contexts. However, a simple combination of RNA FISH and DNA FISH often generates disappointing results because the fragile RNA signals are often damaged by the harsh conditions used in DNA FISH for denaturing the DNA. Here, we describe a robust and simple RNA-DNA FISH protocol, in which amino-labeled nucleic acid probes are used for RNA FISH. The method is suitable to detect single-RNA molecules simultaneously with DNA.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
authors.forename: Lan-Tian
authors.initials: LT
authors.lastname: Lai
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore. fwshao@ntu.edu.sg.
authors.forename: Fangwei
authors.initials: F
authors.lastname: Shao
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore. zhanglf@ntu.edu.sg.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000032:analysis / Q000138:chemical synthesis / Q000235:genetics; D062085:RNA, Long Noncoding / Q000032:analysis* / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D062085:RNA, Long Noncoding; D004247:DNA
keywords: Amino-labeled probes; Fluorescence in situ hybridization (FISH); Simultaneous RNA–DNA FISH; Single-molecule RNA FISH; lncRNA