title: Ultrafast Assessment of the Presence of a High-Risk Mycobacterium tuberculosis Strain in a Population.
abstract: A persistent 8-year infection by a Beijing Mycobacterium tuberculosis strain from a previous outbreak after importation from West Africa obliged us to investigate secondary cases. We developed a multiplex PCR method based on whole-genome sequencing to target strain-specific single nucleotide polymorphisms (SNPs). In 1 week, we analyzed 868 isolates stored over 6 years. Only 2 cases (immigrants from Guinea Conakry) harbored the strain, which ruled out transmission-despite opportunities-and challenged some of the advantages associated with Beijing strains.
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain CIBER Enfermedades Respiratorias, CIBERES, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Lago
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain CIBER Enfermedades Respiratorias, CIBERES, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Herranz
authors.affiliation: Genomics and Health Unit, FISABIO Public Health, Valencia, Spain CIBER Salud Pública CIBERESP, Spain.
authors.forename: Iñaki
authors.initials: I
authors.lastname: Comas
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain CIBER Enfermedades Respiratorias, CIBERES, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Ruiz-Serrano
authors.affiliation: Servicio de Microbiología, Hospital General Universitario Doce de Octubre, Instituto de Investigación Hospital 12 de Octubre, Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: López Roa
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain CIBER Enfermedades Respiratorias, CIBERES, Spain Departamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain CIBER Enfermedades Respiratorias, CIBERES, Spain CEI Campus Moncloa, UCM-UPM, Madrid, Spain dgviedma2@gmail.com.
authors.forename: Darío
authors.initials: D
authors.lastname: García-de-Viedma
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D011159:Population Surveillance; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*