title: Phosphorylated neuronal nitric oxide synthase in neuropathic pain in rats.
abstract: Neuropathic pain caused by nervous system damage or system dysfunction. The pathogenesis and the mechanism underlying neuropathic pain remains unclear. The only known neurobiological component involved in the neuropathic pain is nitric oxide (NO). NO is synthesized by nitric oxide synthase (nNOS) from L-arginine and oxygen. nNOS is involved in the inflammatory pain and neuropathic pain. In this study, we aimed to identify whether KN93 reduced the pain in the rats. Sixty adult male SD rat were randomly divided into 4 groups. Sham group and model group were not received treatment. Experimental group received intrathecal injection of KN93, and negative control group received DMSO injection 30 min before pain test. After last test of pain threshold, the rats were sacrificed and lumbar spinal tissues were sampled for analysis of the expression of pnNOS and pCaMK II by quantitative PCR and Western blotting. Pain threshold was increased in the rats received KN93 treatment (P<0.01), and the expression levels of pnNOS was increased (P<0.05) in experimental group and accompanied with decrease of CaMK II expression (P<0.05). By administration of KN93, the interaction of nNOS and the adaptor protein CAPON was reduced through inhibition of CaMK II by KN93. In conclusion, this study reveals that KN93 can reduce neuropathic pain via inhibiting the activity of CaMK II, and then increase the level of phosphorylated nNOS, to reduce the interaction with CAPON.
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Fumou
authors.initials: F
authors.lastname: Deng
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital to Nanchang University Nanchang 330006, PR China.
authors.forename: Guohai
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000494:pharmacology / Q000627:therapeutic use; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors; D008297:Male; D009437:Neuralgia / Q000188:drug therapy / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000187:drug effects / Q000378:metabolism*; D017288:Pain Threshold / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000494:pharmacology / Q000627:therapeutic use; D000096926:Benzenesulfonamides
chemical_list: D001596:Benzylamines; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D052248:Nitric Oxide Synthase Type I; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; C072105:KN 93; D000096926:Benzenesulfonamides
keywords: CAPON; neuropathic pain; phosphorylation