title: A composite agarose-polyacrylamide matrix as two-dimensional hard support for solid-phase protein assays.
abstract: The solid-phase protein assays using blotting membranes as hard support do not allow achieving the low background and sensitivity of protein staining in clear gels. The membrane opacity complicates imaging of results on standard lab documentation systems. We describe a low-cost transparent matrix that can be used as an alternative to polymeric membranes for solid-phase assays. Protein samples are spotted onto a dry film of composite agarose-polyacrylamide matrix covering standard glass microscopic slides. After rehydration in protein-fixing solution, matrix with protein samples can be detached from glass support and stained as conventional protein polyacrylamide gels.
authors.affiliation: Institute of Developmental Biology of Russian Academy of Sciences, Moscow 119334, Russia. Electronic address: wkrajewski@hotmail.com.
authors.forename: Wladyslaw A
authors.initials: WA
authors.lastname: Krajewski
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D011506:Proteins / Q000032:analysis*; D012685:Sepharose / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013194:Staining and Labeling; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D011506:Proteins; D012685:Sepharose; D012710:Serum Albumin, Bovine; D000099166:Polyacrylamides
keywords: Gel staining; Protein determination; Protein microassay; Solid-phase assay