title: Hyperpolarised organic phosphates as NMR reporters of compartmental pH.
abstract: Organic phosphate metabolites contain functional groups with pKa values near the physiologic pH range, yielding pH-dependent (13)C chemical shift changes of adjacent quaternary carbon sites. When formed in defined cellular compartments from exogenous hyperpolarised (13)C substrates, metabolites can thus yield localised pH values and correlations of organelle pH and catalytic activity.
authors.affiliation: Technical University of Denmark, Department of Electrical Engineering, Ørsteds Plads, Building 348, DK-2800 Kgs. Lyngby, Denmark and Albeda Research, Ole Maaløvs Vej 3, DK-2200 Copenhagen, Denmark.
authors.forename: Pernille Rose
authors.initials: PR
authors.lastname: Jensen
authors.affiliation: Technical University of Denmark, Department of Chemistry, Kemitorvet, Building 207, DK-2800 Kgs. Lyngby, Denmark. semei@kemi.dtu.dk.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Meier
mesh_terms: D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000737:chemistry*
chemical_list: D010710:Phosphates