title: Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro.
abstract: The purpose of the present study was to explore the role and mechanism of extracellular ecto-5'-nucleotidase (CD73) in human colorectal cancer growth. Firstly, CD73 expression was detected in colorectal cancer cell lines both at the mRNA and protein levels. Secondly, recombinant CD73 interference and overexpression lentiviruses were used, respectively. Colony formation assay, CCK-8 assay and flow cytometry were used to investigate the impact of CD73 on colorectal cancer cell proliferation and cell cycle distribution. Then, adenosine and CD73 enzyme activity inhibitor (APCP) were used to study the effect of CD73 on Epidermal growth factor receptor (EGFR) and β-catenin/cyclin D1 signaling pathways. Finally, a human colorectal cancer transplantation nude mouse model was used to observe the effect of CD73 on tumor growth in vivo. As the results showed, CD73 was highly expressed in the colorectal cancer cell lines. CD73 promoted colorectal cancer cell proliferation both in vivo and in vitro. CD73 activated EGFR and the β-catenin/cyclin D1 signaling pathways through its enzyme and non-enzyme activities. All of the results confirmed that CD73 promotes the growth of human colorectal cancer cells through EGFR and the β-catenin/cyclin D1 signaling pathway. CD73 may be used as a valuable biomarker of colorectal cancer.
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Yijia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Fuyan
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Pei
mesh_terms: D015720:5'-Nucleotidase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics*; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D034741:RNA, Small Interfering / Q000008:administration & dosage; D015398:Signal Transduction; D051176:beta Catenin / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D004791:Enzyme Inhibitors; D058851:GPI-Linked Proteins; D034741:RNA, Small Interfering; D051176:beta Catenin; D019938:Cyclin D1; C512478:EGFR protein, human; D066246:ErbB Receptors; D015720:5'-Nucleotidase; C548241:NT5E protein, human