title: Bifidobacterium aquikefirisp. nov., isolated from water kefir.
abstract: A novelBifidobacterium, strain LMG 28769T, was isolated from a household water kefir fermentation process. Cells were Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, oxidase-negative and facultatively anaerobic short rods. Analysis of its 16S rRNA gene sequence revealedBifidobacterium crudilactisandBifidobacterium psychraerophilum(97.4 and 97.1 % similarity towards the respective type strain sequences) as nearest phylogenetic neighbours. Its assignment to the genusBifidobacteriumwas confirmed by the presence of fructose 6-phosphate phosphoketolase activity. Analysis of thehsp60gene sequence revealed very low similarity with nucleotide sequences in the NCBI nucleotide database. The genotypic and phenotypic analyses allowed the differentiation of strain LMG 28769Tfrom all recognizedBifidobacteriumspecies. Strain LMG 28769T( = CCUG 67145T= R 54638T) therefore represents a novel species, for which the nameBifidobacterium aquikefirisp. nov. is proposed.
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University,K. L., Ledeganckstraat 35, B-9000 Ghent,Belgium.|Research Group of Industrial Microbiology and Food Biotechnology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel,Pleinlaan 2, B-1050, Brussels,Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Laureys
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University,K. L., Ledeganckstraat 35, B-9000 Ghent,Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Research Group of Industrial Microbiology and Food Biotechnology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel,Pleinlaan 2, B-1050, Brussels,Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: De Vuyst
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University,K. L., Ledeganckstraat 35, B-9000 Ghent,Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme