title: miR-146b-5p promotes VSMC proliferation and migration.
abstract: Vascular smooth muscle cells (VSMCs) play pivotal roles in the development of vascular diseases. While microRNAs are important in vascular pathologies, a few is known about their functional roles in VSMC phenotypes. We profiled microRNA expression in PDGF-BB treated VSMCs and found microRNA-146b-5p (miR-146b-5p) was upregulated. Inhibition of miR-146b-5p blocked in response to PDGF while reducing VSMC proliferation and migration. These studies implicate miR-146b-5p as necessary for PDGF-induced VSMC phenotype transition. Downstream miR-146b-5p targets modulating VSMC phenotypes will be further identified. Our study will help to understand the role of VSMCs in the pathology of vascular diseases.
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Peihua
authors.initials: P
authors.lastname: Gong
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital Shanghai, China.
authors.forename: Changwu
authors.initials: C
authors.lastname: Ruan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D051379:Mice; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D035683:MicroRNAs; C534190:Mirn146 microRNA, mouse
keywords: miR-146b-5p; migration; proliferation; vascular smooth muscle cells