title: Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells.
abstract: The aim of the present study was to examine whether the intestine gastrointestinal (GI) digests of abalone [Haliotis discus hannai (H. discus hannai)] modulate inflammatory responses and to elucidate the mechanisms involved. The GI digests of the abalone intestines were fractionated into fractions I (>10 kDa), II (5-10 kDa) and Ⅲ (<5 kDa). Of the abalone intestine GI digests (AIGIDs), fraction Ⅲ inhibited the passive cutaneous anaphylaxis (PCA) reaction in mice. Subsequently, a bioactive peptide [abalone intestine GI digest peptide (AIGIDP)] isolated from fraction Ⅲ was determined to be 1175.2 Da, and the amino acid sequence was found to be PFNQGTFAS. We noted that the purified nonameric peptide (AIGIDP) attenuated the phorbol‑12‑myristate 13-acetate plus calcium ionophore A23187 (PMACI)-induced histamine release and the production of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in human mast cells (HMC-1 cells). In addition, we also noted that AIGIDP inhibited the PMACI‑induced activation of nuclear factor‑κB (NF-κB) by suppressing IκBα phosphorylation and that it suppressed the production of cytokines by decreasing the phosphorylation of JNK. The findings of our study indicate that AIGIDP exerts a modulatory, anti-allergic effect on mast cell-mediated inflammatory diseases.
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea.
authors.forename: Seok-Chun
authors.initials: SC
authors.lastname: Ko
authors.affiliation: Converging Research Division, National Marine Biodiversity Institute of Korea, Seochun, Chungcheongnam-do, Republic of Korea.
authors.forename: Dae-Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Gangwon, Republic of Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.affiliation: Converging Research Division, National Marine Biodiversity Institute of Korea, Seochun, Chungcheongnam-do, Republic of Korea.
authors.forename: Jong Su
authors.initials: JS
authors.lastname: Yoo
authors.affiliation: Converging Research Division, National Marine Biodiversity Institute of Korea, Seochun, Chungcheongnam-do, Republic of Korea.
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Yim
authors.affiliation: Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong, P.R. China.
authors.forename: Zhong-Ji
authors.initials: ZJ
authors.lastname: Qian
authors.affiliation: Department of Molecular Microbiology and Immunology, Warren Alpert School of Medicine Box G-L, Providence, RI, USA.
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lee
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea.
authors.forename: Junghwan
authors.initials: J
authors.lastname: Oh
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea.
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea.
authors.forename: Il-Whan
authors.initials: IW
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D041981:Gastrointestinal Tract / Q000737:chemistry; D049851:Gastropoda / Q000737:chemistry*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D018836:Inflammation Mediators / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008813:Mice, Inbred ICR; D020928:Mitogen-Activated Protein Kinases / Q000276:immunology; D016328:NF-kappa B / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D018926:Anti-Allergic Agents; D001688:Biological Products; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D010455:Peptides; D007073:Immunoglobulin E; D020928:Mitogen-Activated Protein Kinases