title: Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen.
abstract: In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05-0.50µgmL-1and a detection limit down to 0.035µgmL-1as compared to its non-sandwich counterpart, which yielded a linear range of 0.05-0.40µgmL-1, with a detection limit of 0.042µgmL-1. The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor.
authors.affiliation: Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: S X
authors.initials: SX
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Functional Device Laboratory, Institute of Advanced Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: H N
authors.initials: HN
authors.lastname: Lim
authors.affiliation: Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Ibrahim
authors.affiliation: Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: A
authors.initials: A
authors.lastname: Jamil
authors.affiliation: Low Dimensional Materials Research Centre (LDMRC), Physics Department, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: A
authors.initials: A
authors.lastname: Pandikumar
authors.affiliation: Low Dimensional Materials Research Centre (LDMRC), Physics Department, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: N M
authors.initials: NM
authors.lastname: Huang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; D010087:Oxides; D012834:Silver; D006108:Graphite; D006735:Horseradish Peroxidase
keywords: Carcinoembryonic antigen; Electrochemical sensor; Graphene; Immunosensor; Silver nanoparticle