title: MiR-145 facilitates proliferation and migration of endothelial progenitor cells and recanalization of arterial thrombosis in cerebral infarction mice via JNK signal pathway.
abstract: Arterial thrombosis in cerebral infarction severely affects patients' lives. Classical treatment including surgery and medication both had significantly adverse effects, making it necessary to find novel strategy. Endothelial progenitor cells (EPCs) have been shown to enhance the recanalization of thrombosis, while leaving its molecular mechanism unclear. EPCs were separated from peripheral blood, and were transfected by microRNA (miR)-145. The growth, proliferation and migration abilities were quantified by MTT, clone formation and Transwell assays, respectively. Cell apoptosis was evaluated by flow cytometry. The activation of JNK signaling pathway was measured by Western blotting, followed by JNK inhibitor SP600125. In a mouse cerebral infarction model, miR-145 transfected EPCs were injected to observe the condition of arterial thrombosis. MiR-145 transfection enhanced growth, migration and proliferation of EPCs without induction of apoptosis. MiR-145 exerts its effects via JNK signaling pathway, as the blocking inhibited cell migration/proliferation. In vivo injection of miR-145 transfected EPCs also potentiated cell proliferation and migration, in addition to the recanalization of arterial thrombosis. MiR-145 facilitates proliferation and migration of EPCs and recanalization of arterial thrombosis in cerebral infarction mice via JNK signal pathway. This study provided new insights regarding infarction treatment.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Shantou University Medical College Shantou 515041, Guangdong, China.
authors.forename: Rongbo
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Shantou University Medical College Shantou 515041, Guangdong, China.
authors.forename: Siqia
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Shantou University Medical College Shantou 515041, Guangdong, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liao
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Shantou University Medical College Shantou 515041, Guangdong, China.
authors.forename: Xiaopu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Nursing, The First Affiliated Hospital of Shantou University Medical College Shantou 515041, Guangdong, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D066026:Endothelial Progenitor Cells / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013927:Thrombosis / Q000378:metabolism*
chemical_list: C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs
keywords: JNK signal pathway; MicroRNA-145; cerebral infarction; endothelial progenitor cells