title: Transcriptome and biomineralization responses of the pearl oyster Pinctada fucata to elevated CO2 and temperature.
abstract: Ocean acidification and global warming have been shown to significantly affect the physiological performances of marine calcifiers; however, the underlying mechanisms remain poorly understood. In this study, the transcriptome and biomineralization responses of Pinctada fucata to elevated CO2 (pH 7.8 and pH 7.5) and temperature (25 °C and 31 °C) are investigated. Increases in CO2 and temperature induced significant changes in gene expression, alkaline phosphatase activity, net calcification rates and relative calcium content, whereas no changes are observed in the shell ultrastructure. "Ion and acid-base regulation" related genes and "amino acid metabolism" pathway respond to the elevated CO2 (pH 7.8), suggesting that P. fucata implements a compensatory acid-base mechanism to mitigate the effects of low pH. Additionally, "anti-oxidation"-related genes and "Toll-like receptor signaling", "arachidonic acid metabolism", "lysosome" and "other glycan degradation" pathways exhibited responses to elevated temperature (25 °C and 31 °C), suggesting that P. fucata utilizes anti-oxidative and lysosome strategies to alleviate the effects of temperature stress. These responses are energy-consuming processes, which can lead to a decrease in biomineralization capacity. This study therefore is important for understanding the mechanisms by which pearl oysters respond to changing environments and predicting the effects of global climate change on pearl aquaculture.
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.|Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Yangjia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Guilan
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.affiliation: Institute of Marine Biotechnology, Collaborative Innovation Center of Deep Sea Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Rongqing
authors.initials: R
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry; D060105:Animal Shells / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic; D002245:Carbon Dioxide; D057231:Climate Change; D020869:Gene Expression Profiling; D057232:Global Warming; D008954:Models, Biological; D049911:Pinctada / Q000502:physiology*; D013312:Stress, Physiological; D013696:Temperature; D059467:Transcriptome
chemical_list: D000596:Amino Acids; D002245:Carbon Dioxide; D000469:Alkaline Phosphatase