title: Cry1ab/c in different stages of growth in transgenic rice Bt-shanyou63.
abstract: The relationship between the mRNA level and the corresponding protein level of the cry1Ab/c gene is not well characterized in transgenic rice (Bt-ShanYou63). In this study, we compared cry1Ab/c mRNA and its protein expression in leaves at different growth stages in Bt-ShanYou63 rice. The results demonstrated that both cry1Ab/c mRNA and its protein levels changed at all of the growth stages. The cry1Ab/c transcript levels in the leaves were highest during the grain filling stage (3.29, cry/actin) and lowest during the seeding stage (1.06, cry/actin), and the protein levels of Cry1Ab/c was also highest at the grain filling stage (5.71 microgram x g-1 fresh weight, fw) and lowest during the seeding stage (2.08 microgram x g(-1) fw). There was a significant correlation between cry1Ab/c mRNA levels and the protein concentrations (r=0.742, p < 0.01). However, a linear relationship was not observed between cry1Ab/c mRNA levels and the protein levels, and the trend for mRNA expression levels was not consistent with the Cry1Ab/c protein levels in the same growth period in Bt-ShanYou63 rice.
authors.affiliation: Nanjing Institute of Environmental Sciences(NIES).
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Shen
authors.affiliation: tate Key Laboratory of Biosafty, Nanjing Institute of Environmental Sciences(NIES), Ministry of Environmental Protection of China, No 8, Jiang-wang-miao St., Nanjing, Jiangsu 210042, China, 2College of Life Science, Nanjing Agricultural University, Nanjin.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Fang
authors.affiliation: China Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China, liubiao@pku.org.cn.
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
mesh_terms: D017343:Genes, Plant; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger