title: Intraventricular administration of endoneuraminidase-N facilitates ectopic migration of subventricular zone-derived neural progenitor cells into 6-OHDA lesioned striatum of mice.
abstract: Polysialic acid (PSA), a carbohydrate polymer associated with the neural cell adhesion molecule (NCAM), plays an important role in the migration, differentiation and maturation of neuroblasts. Endoneuraminidase-N (Endo-N) can specifically cleave PSA from NCAM. The objective of the present study was to examine: the effect of Endo-N on characteristics of subventricular zone (SVZ)-derived neural progenitor cells (NPCs) in vitro; whether intraventricular administration of Endo-N could increase ectopic migration of SVZ-derived NPCs into 6-hydroxydopamine (6-OHDA)-lesioned striatum, and whether migrated NPCs could differentiate into neuronal and glial cells. In in vitro study, Endo-N was found to inhibit the migration of NPCs, and to enhance the differentiation of NPCs. In in vivo study, mice sequentially received injections of 6-OHDA into the right striatum, Endo-N into the right lateral ventricle, and bromodeoxyuridine (BrdU) intraperitoneally. The data showed that intraventricular injections of Endo-N disorganized the normal structure of the rostral migratory stream (RMS), and drastically increased the number of BrdU-immunoreactive (IR) cells in 6-OHDA-lesioned striatum. In addition, a number of BrdU-IR cells were double labeled for doublecortin (DCX), NeuN or glial fibrillary acidic protein (GFAP). The results suggest that interruption of neuroblast chain pathway with Endo-N facilitates ectopic migration of SVZ-derived NPCs into the lesioned striatum, and migrated NPCs can differentiate into neurons and astrocytes.
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hao
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Chen
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hao
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Qu
authors.affiliation: Department of Neurosurgery, Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: Li-Ru
authors.initials: LR
authors.lastname: Zhao
authors.affiliation: Department of Anatomy, Capital Medical University, Beijing 100069, China; Center of Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100069, China. Electronic address: duanwm08@yahoo.com.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Duan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D020520:Brain Infarction / Q000139:chemically induced / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000473:pathology*; D000089662:Doublecortin Protein; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D020547:Lateral Ventricles / Q000166:cytology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects*; D016627:Oxidopamine / Q000633:toxicity; D012794:Sialic Acids / Q000378:metabolism; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D005904:Glial Fibrillary Acidic Protein; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; D014404:Tubulin; C462941:beta3 tubulin, mouse; C103469:polysialyl neural cell adhesion molecule; D016627:Oxidopamine; D006026:Glycoside Hydrolases; C090534:endo-alpha-sialidase
keywords: 6-Hydroxydopamine; Endoneuraminidase-N; Migration; Neural progenitor cells; Polysialic acid-neural cell adhesion molecule; Striatum; Subventricular zone