title: Mapping of Membrane Lipid Order in Root Apex Zones of Arabidopsis thaliana.
abstract: In this study, we used the fluorescence probe, Di-4-ANEPPDHQ, to map the distribution of membrane lipid order in the apical region of Arabidopsis roots. The generalized polarization (GP) value of Di-4-ANEPPDHQ-stained roots indicated the highest lipid order in the root transition zone (RTZ). The cortical cells have higher lipid order than the epidermal cells in same regions, while the developing root hairs show very prominent cell polarity with high lipid order in apical region. Moreover, the endosomes had lower lipid order than that of the plasma membrane (PM). Brefeldin A (BFA) treatment decreased the lipid order in both the plasma and endosomal membranes of epidermal cells in the RTZ. The lipid order of BFA-induced compartments became higher than that of the PM after BFA treatment in epidermal cells. Meanwhile, the polarly growing tips of root hairs did not show the same behavior. The lipid order of the PM remained unchanged, with higher values than that of the endosomes. This suggests that the lipid ordering in the PM was affected by recycling of endosomal vesicles in epidermal cells of the root apex transition zone but not in the root hairs of Arabidopsis.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing, China.
authors.forename: Xiran
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing, China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Cellular and Molecular Botany, University of Bonn Bonn, Germany.
authors.forename: František
authors.initials: F
authors.lastname: Baluška
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing, China.
authors.forename: Yinglang
authors.initials: Y
authors.lastname: Wan
keywords: Di-4-ANEPPDHQ; membrane microdomains; membrane order; root apex; root transition zone