title: Obscurumines H-P, new Lycopodium alkaloids from the club moss Lycopodium obscurum.
abstract: Seven new fawcettimine-type (1-7) and two new lycopodine-type (8 and 9) Lycopodium alkaloids, as well as 10 known compounds, were isolated from the club moss, Lycopodium obscurum L. The structures of obscurumines H-P (1-9) were determined based on high-resolution MS and 1D and 2D NMR data. Compounds 1 and 2 include a new skeleton that is formed via the linkage of C-9-N-2', which is rarely present in Lycopodium alkaloids. The in vitro acetylcholinesterase (AChE) inhibitory activity assay showed that 5 exhibited weak anti-AChE activity with an IC50 value of 81.0 μM. Compound 8 exhibited inhibition of the secretion of IL-2 in phytohemagglutinin (PHA) and phorbol myristate acetate (PMA) stimulated Jurkat cells, and the IC50 value was 17.2 μM.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China; College of Basic Science and Information Engineering, Yunnan Agricultura University, Kuming 6540201, PR China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Su
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Li-Dong
authors.initials: LD
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Xing-De
authors.initials: XD
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China. Electronic address: qinshizhao@mail.kib.ac.cn.
authors.forename: Qin-Shi
authors.initials: QS
authors.lastname: Zhao
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000302:isolation & purification; D019169:Jurkat Cells; D032502:Lycopodium / Q000737:chemistry*; D015394:Molecular Structure; D011807:Quinolizines / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D007166:Immunosuppressive Agents; D011807:Quinolizines; C000608986:obscurumine L; C000608987:obscurumine O; D000110:Acetylcholinesterase
keywords: Anti-AChE activity; Immunosuppressive activity; Lycopodium alkaloids; Lycopodium obscurum L.; Obscurumines H-P