title: Enhanced succinic acid productivity by expression of mgtCB gene in Escherichia coli mutant.
abstract: In this study, a novel engineering Escherichia coli strain (CBMG111) with the expression of mgtCB gene was constructed for the enhanced fermentative production of succinic acid by utilizing the synergetic effect of mgtC gene to improve the growth of strains at the environment of low Mg(2+) concentration and mgtB to enhance the transport of Mg(2+) into cells. After the effect of the expression of the individual genes (mgtA, mgtB, mgtC) on the growth of E. coli was clarified, the fermentative production of succinic acid by CBMG111 was studied with the low-price mixture of Mg(OH)2 and NH3·H2O as the alkaline neutralizer and the biomass hydrolysates as the carbon sources, which demonstrated that the expression of mgtCB gene can significantly increase the productivity of succinic acid (2.97 g L(-1) h(-1)) compared with that by using the engineering strain with the overexpression of mgtA gene.
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, P.O. Box 94#, Shazheng Street 174, Shapingba District, Chongqing, 400044, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, P.O. Box 94#, Shazheng Street 174, Shapingba District, Chongqing, 400044, China.
authors.forename: Le
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, P.O. Box 94#, Shazheng Street 174, Shapingba District, Chongqing, 400044, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, P.O. Box 94#, Shazheng Street 174, Shapingba District, Chongqing, 400044, China. lcdong72@cqu.edu.cn.
authors.forename: Lichun
authors.initials: L
authors.lastname: Dong
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, 30332, USA. rachel.chen@chbe.gatech.edu.
authors.forename: Rachel
authors.initials: R
authors.lastname: Chen
mesh_terms: D017920:Antiporters / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D008274:Magnesium / Q000378:metabolism; D009154:Mutation; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D019802:Succinic Acid; D008274:Magnesium
keywords: Alkaline neutralizer; Magnesium transporter; MgtC; Productivity; Succinic acid