title: GPX4 and GPX7 over-expression in human hepatocellular carcinoma tissues.
abstract: Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. The selenium is an essential trace mineral implicated as a key factor in the early stage of cancer and exerts its biological function through the selenoproteins. In the last years our group has been studying the involvement of some selenoproteins in HCC. However, no many data are reported in literature about the correlation between HCC and the glutathione peroxidases (GPXs), both selenium and non selenium-containing GPXs. In this paper we have evaluated the GPX4 and GPX7 expression in some paraffin-embedded tissues from liver biopsy of patients with hepatitis C virus (HCV)-related cirrhosis and HCC by immunohistochemistry and RT-qPCR analysis. Our results evidenced that i) GPX4 and GPX7 had a statistically significant over-expression in HCC tissues compared to cirrhotic counterparts used as non tumor tissues, and ii) their expression was higher in grade III HCC tissues with respect to grade I-II samples. Therefore, we propose to use GPX4 and GPX7 as possible markers for improving HCC diagnosis/prognosis.
authors.affiliation: Istituto Nazionale Tumori "Fondazione Giovanni Pascale", IRCCS. eliana.guerriero@cro-m.eu.
authors.forename: E
authors.initials: E
authors.lastname: Guerriero
authors.forename: F
authors.initials: F
authors.lastname: Capone
authors.forename: M
authors.initials: M
authors.lastname: Accardo
authors.forename: A
authors.initials: A
authors.lastname: Sorice
authors.forename: M
authors.initials: M
authors.lastname: Costantini
authors.forename: G
authors.initials: G
authors.lastname: Colonna
authors.forename: G
authors.initials: G
authors.lastname: Castello
authors.forename: S
authors.initials: S
authors.lastname: Costantini
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010544:Peroxidases / Q000096:biosynthesis*; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D010544:Peroxidases; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; C558641:GPX7 protein, human; D005979:Glutathione Peroxidase