title: Dental Hetero-Graft Materials with Nano Hydroxyapatite Surface Treatment.
abstract: We report the development of hydroxyapatite nanoparticle (HAp NP)-functionalized hetero-graft materials (HGMs) for dental applications. These HGMs were prepared by attaching platelet-, needle-, and sphere-shaped HAp NPs to the surface of xenograft materials through chemical conjugation. Although all three HAp NPs contributed to increase the surface area of bone graft material (BGM), the shape of the HAp NPs was a determining factor. Platelet HAp NPs were most effective, because they caused a 48.9% increase in BGM surface area whereas the influence of the spherical NP was only a 6.7% increase. This suggests that geometric factors regarding both the attached HAp NPs and graft material surface are essential in controlling the surface roughness of graft materials. Among the three HAp NPs, it was the platelet HAp NPs that helped to increase the efficacy of the BGM most significantly. Compared with BGM with no HAp NP attachment, HGM with platelet HAp NP ('platelet-HGM) treatment had ~46.1% higher cell attachment and proliferation rate. The MTT assay also showed that the HAp NP-treated hetero-graft materials had negligible cytotoxicity.
authors.forename: Dai-Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Kyung-Il
authors.initials: KI
authors.lastname: Kim
authors.forename: Seokyoung
authors.initials: S
authors.lastname: Yoon
authors.forename: Hyung-Jo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Ahn
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jun
authors.forename: Ho Chang
authors.initials: HC
authors.lastname: Kang
authors.forename: Changhyun
authors.initials: C
authors.lastname: Pang
authors.forename: Jaeyun
authors.initials: J
authors.lastname: Kim
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
authors.forename: Kwon-Hoon
authors.initials: KH
authors.lastname: Han
authors.forename: Dong-Myong
authors.initials: DM
authors.lastname: Kim
authors.forename: Jung Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D015921:Dental Implants; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D013499:Surface Properties; D013552:Swine
chemical_list: D015921:Dental Implants; D017886:Durapatite