title: Autophagy Promotes Microglia Activation Through Beclin-1-Atg5 Pathway in Intracerebral Hemorrhage.
abstract: Previous study demonstrates that intracerebral hemorrhage (ICH) promotes microglia activation and inflammation. However, the exact mechanism of microglia activation induced by ICH is not clear. In this experiment, microglia autophagy was examined using electron microscopy, conversion of light chain 3(LC3), and monodansylcadaverine (MDC) staining to detect autophagic vacuoles. We found that ICH induced microglia autophagy and activation. The suppression of autophagy using either pharmacologic inhibitors (3-methyladenine, bafilomycin A1) or RNA interference in essential autophagy genes (BECN1 and ATG5) decreased the microglia activation and inflammation in ICH. Moreover, autophagy inhibitors reduced brain damage in ICH. In conclusion, these data indicate that ICH contributes to microglia autophagic activation through BECN1 and ATG5 and provide the therapeutical strategy for ICH.
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian, 350025, China.
authors.forename: Bangqing
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian, 350025, China.
authors.forename: Hanchao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian, 350025, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian, 350025, China.
authors.forename: Tonggang
authors.initials: T
authors.lastname: Su
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China. yangzhao5140@sohu.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D001343:Autophagy / Q000187:drug effects / Q000502:physiology*; D000071187:Autophagy-Related Protein 5 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000071186:Beclin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018942:Macrolides / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: C495773:Atg5 protein, mouse; D000071187:Autophagy-Related Protein 5; D000071186:Beclin-1; D018942:Macrolides; C025946:3-methyladenine; C040929:bafilomycin A1; D000225:Adenine
keywords: Atg5; Autophagy; Beclin-1; ICH; Microglia