title: Cytokine Expression Pattern in Bone Marrow Microenvironment after Allogeneic Stem Cell Transplantation in Primary Myelofibrosis.
abstract: The only curative therapy for primary myelofibrosis (PMF) is allogeneic stem cell transplantation (ASCT). However, although we know that patients can benefit from ASCT, we do not know the extent of the changes of the expression profile of cytokines and matrix modulation factors. In this first systematic analysis, we evaluated the expression profile of 103 factors before and after transplantation to identify potential biomarkers. The expression of fibrosis-, inflammation-, and angiogenesis-associated genes was analyzed in a total of 52 bone marrow biopsies: PMF patients (n = 14) before and after ASCT and, for control purposes, post-ASCT multiple myeloma patients (n = 14) and non-neoplastic hematopoiesis (n = 10). In post-ASCT PMF cases, decreased expression of tissue inhibitor of metalloproteinases (TIMP) and platelet-derived growth factor alpha (PDGFA) correlated with bone marrow remodeling and hematological remission. Expression of several other matrix factors remained at high levels and may contribute to post-ASCT remodeling. This is the first systematic analysis of cytokine expression in post-ASCT PMF bone marrow that shows that normalization of bone marrow microenvironment is paralleled by decreased expression of TIMP and PDGFA.
authors.affiliation: Institute of Pathology, Hannover Medical School, Hannover, Germany. Electronic address: Hussein.Kais@MH-Hannover.de.
authors.forename: Kais
authors.initials: K
authors.lastname: Hussein
authors.affiliation: Institute of Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Stucki-Koch
authors.affiliation: Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Haefaa
authors.initials: H
authors.lastname: Alchalby
authors.affiliation: Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Triviai
authors.affiliation: Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Nicolaus
authors.initials: N
authors.lastname: Kröger
authors.affiliation: Institute of Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Kreipe
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D060833:Cellular Microenvironment / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D006410:Hematopoiesis / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000276:immunology*; D055728:Primary Myelofibrosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D012189:Retrospective Studies; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines; D016326:Extracellular Matrix Proteins; D010982:Platelet-Derived Growth Factor; D019714:Tissue Inhibitor of Metalloproteinases; C075278:platelet-derived growth factor A
keywords: Allogeneic stem cell transplantation; Fibrosis; Primary myelofibrosis (PMF)