title: Protein phosphatase 2A plays an important role in migration of bone marrow stroma cells.
abstract: Administration of bone marrow stroma cells (BMSCs) has the potential to ameliorate degenerative disorders and to repair injured sites. The homing of transplanted BMSCs to damaged tissues is a critical property of engraftment. Therefore, it is important to understand signal molecules controlling migration of BMSCs. Here, we demonstrate that serine-threonine protein phosphatase 2A (PP2A) is responsive to migration of BMSCs. Pharmacological Inhibition of PP2A, using okadaic acid (OA), leads to attenuated cell migration in rat primary BMSCs both in the absence or presence of stromal cell-derived factor-1 (SDF-1). Consistent with the above findings, knockdown of the main catalytic subunit PP2Acα using small interfering RNA also attenuates chemotaxis of BMSCs. On the other hand, cell viability of BMSCs remains unchanged with OA treatment or knockdown of PP2Acα subunit. Moreover, we observed an upregulation of PP2A-B55β in transcription level after SDF-1 treatment, indicating their potential role as the functioning regulatory subunit of PP2A phosphatase in BMSCs migration model. Collectively, these data provide first insight into the modulation of BMSCs migration by PP2A phosphatase activity and lay a foundation for exploring PP2A signaling as a modulating target for BMSCs transplantation.
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou, 215006, Jiangsu, China.
authors.forename: Weiqian
authors.initials: W
authors.lastname: Chen
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou, 215006, Jiangsu, China.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou, 215006, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.affiliation: Jiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agriculture University, Nanjing, 210095, Jiangsu, China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, 210061, Jiangsu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tu
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou, 215006, Jiangsu, China. uuzyshen@aliyun.com.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Shen
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000502:physiology; D055785:Gene Knockdown Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D019319:Okadaic Acid / Q000494:pharmacology; D054648:Protein Phosphatase 2 / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; D019319:Okadaic Acid; D054648:Protein Phosphatase 2
keywords: Bone marrow stroma cells; Cell migration; Protein phosphatase 2A; Stromal cell-derived factor-1