title: Surface topography of hydroxyapatite promotes osteogenic differentiation of human bone marrow mesenchymal stem cells.
abstract: Effective and safe induction of osteogenic differentiation is one of the key elements of bone tissue engineering. Surface topography of scaffold materials was recently found to promote osteogenic differentiation. Utilization of this topography may be a safer approach than traditional induction by growth factors or chemicals. The aim of this study is to investigate the enhancement of osteogenic differentiation by surface topography and its mechanism of action. Hydroxyapatite (HA) discs with average roughness (Ra) of surface topography ranging from 0.2 to 1.65 μm and mean distance between peaks (RSm) ranging from 89.7 to 18.6 μm were prepared, and human bone-marrow mesenchymal stem cells (hBMSCs) were cultured on these discs. Optimal osteogenic differentiation was observed on discs with surface topography characterized by Ra ranging from 0.77 to 1.09 μm and RSm ranging from 53.9 to 39.3 μm. On this surface configuration of HA, hBMSCs showed oriented attachment, F-actin arrangement, and a peak in the expression of Yes-associated protein (YAP) and PDZ binding motif (TAZ) (YAP/TAZ). These results indicated that the surface topography of HA promoted osteogenic differentiation of hBMSCs, possibly by increasing cell attachment and promoting the YAP/TAZ signaling pathway.
authors.affiliation: Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, PR China.
authors.forename: Wanlei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, PR China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, PR China.
authors.forename: Jianlei
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310027, PR China.
authors.forename: Jirong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Research Centre for Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Haotian
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, PR China. Electronic address: doctor120@hotmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qian
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D017886:Durapatite
keywords: Hydroxyapatite; Osteogenic differentiation; Surface topography; YAP/TAZ; hBMSCs