title: Therapeutic effects of trehalose liposomes against lymphoblastic leukemia leading to apoptosis in vitro and in vivo.
abstract: Inhibitory effects of trehalose liposomes (DMTre) composed of 30mol% l-α-dimyristoylphosphatidylcholine (DMPC) and 70mol% trehalose surfactants on the growth of lymphoblastic leukemia (MOLT-4) cells in vitro and therapeutic effects of DMTre for xenograft mice model of carcinoma in vivo were examined. DMTre inhibited the growth of MOLT-4 cells in a dose-dependent manner due to apoptosis. The activation of caspase-3, -8, and 9 was obtained for MOLT-4 cells after the treatment with DMTre. The clustering of lipid rafts in plasma membranes of MOLT-4 cells was examined with a marker Cholera toxin subunit B conjugates Alexa Fluor (CTB), which binds to the pentasaccharide chains of ganglioside GM1 on the cellular surfaces. The clustering of lipid rafts in plasma membranes of MOLT-4 cells was observed after the treatment with DMTre. Therapeutic effects of DMTre were obtained for xenograft mice model of carcinoma in vivo.
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1, Ikeda, Nishi-ku, Kumamoto 860-0082, Japan. Electronic address: matumoto@life.sojo-u.ac.jp.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1, Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Kuwabara
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1, Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ichihara
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1, Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Kuwano
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000627:therapeutic use*; D020536:Enzyme Activators / Q000737:chemistry / Q000627:therapeutic use; D006017:Glycolipids / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000627:therapeutic use*; D008560:Membrane Fluidity; D021962:Membrane Microdomains; D008564:Membrane Potentials; D051379:Mice; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D020536:Enzyme Activators; D006017:Glycolipids; D008081:Liposomes; C000613563:glycopyranosyl-(1-1)-glucopyranoside 6-O-monomyristate; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D004134:Dimyristoylphosphatidylcholine
keywords: Apoptosis; Caspase; Clustering of lipid rafts; In vivo; Leukemia; Trehalose liposomes