title: Immunohistochemical detection of STAT6, CD34, CD99 and BCL-2 for diagnosing solitary fibrous tumors/hemangiopericytomas.
abstract: Solitary fibrous tumors (SFTs)/hemangiopericytomas (HPCs) are uncommon mesenchymal neoplasms of fibroblastic type that can arise anywhere in the body. Recently, NGFI-A binding protein 2 (NAB2)-signal transducer and the activator of transcription 6 (STAT6) fusion gene were discovered as a hallmark of SFTs/HPCs by using whole-exome, and transcriptome sequencing; consequently, the fusion gene can be rapidly detected by STAT6 immunohistochemistry. In this study, 53 formalin-fixed, paraffin-embedded (FFPE) tissues were performed using immunohistochemistry with antibodies against STAT6, CD34, CD99 and Bcl-2. Nuclear STAT6 positive staining was present in 51 cases (51/53, sensitivity 96.2%), which were usually diffuse (4+ in 14 cases; 3+ in 13 cases; 2+ in 9 cases; 1+ in 15 cases) and intense (strong in 17 cases; moderate in 22 cases; and weak in 12 cases) staining. CD34 was positive in 47 cases (47/53, sensitivity 88.7%), CD99 was positive in 50 cases (50/53, sensitivity 94.3%) and Bcl-2 was positive in 51 cases (51/53, sensitivity 96.2%). There is no difference among categories such as age, sex, location, tumor size, or estimated dignity in immunohistochemical staining of STAT6, CD34, CD99 and Bcl-2. The nuclear STAT6 being positive is a helpful and highly sensitive marker in diagnosis of SFTs/HPCs. Considering immunohistochemical STAT6, CD34, CD99 and Bcl-2 findings together can provide more supportive diagnostic information.
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Yunan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Pathology, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Qingfu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Pathology, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pathology, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
mesh_terms: D000071443:12E7 Antigen; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis; D005260:Female; D006393:Hemangiopericytoma / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D050800:STAT6 Transcription Factor / Q000032:analysis; D012680:Sensitivity and Specificity; D054364:Solitary Fibrous Tumors / Q000175:diagnosis* / Q000378:metabolism; D055815:Young Adult
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D019253:Proto-Oncogene Proteins c-bcl-2; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human
keywords: Solitary fibrous tumor; hemangiopericytoma; immunohistochemistry