title: Suppression of TPA-induced cancer cell invasion by Peucedanum japonicum Thunb. extract through the inhibition of PKCα/NF-κB-dependent MMP-9 expression in MCF-7 cells.
abstract: Metastatic cancers spread from their site of origin (the primary site) to other parts of the body. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix, is important in metastatic cancers as it plays a major role in cancer cell invasion. The present study examined the inhibitory effect of an ethanol extract of Peucedanum japonicum Thunb. (PJT) on MMP-9 expression and the invasion of MCF-7 breast cancer cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Western blot analysis, gelatin zymography, and reverse transcription-quantitative PCR revealed that PJT significantly suppressed MMP-9 expression and activation in a dose-dependent manner. Furthermore, PJT attenuated TPA-induced nuclear translocation and the transcriptional activation of nuclear factor (NF)-κB. The results indicated that the PJT-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involved the suppression of the PKCα/NF-κB pathway in MCF-7 cells. Thus, the inhibition of MMP-9 expression by PJT may have potential value as a therapy for restricting the invasiveness of breast cancer.
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Noh
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Ha-Rim
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Mi-Seong
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Song
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Minok
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Sei-Hoon
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Department of Herbology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Guem-San
authors.initials: GS
authors.lastname: Lee
authors.affiliation: Institute of Customized Physical Therapy, Gwanju Metropolitan City 506-303, Republic of Korea.
authors.forename: Hyoung-Chul
authors.initials: HC
authors.lastname: Moon
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Kang-Beom
authors.initials: KB
authors.lastname: Kwon
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D001940:Breast / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002273:Carcinogens / Q000633:toxicity; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002273:Carcinogens; D016328:NF-kappa B; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate