title: Development of neuraminidase detection using gold nanoparticles boron-doped diamond electrodes.
abstract: Gold nanoparticles-modified boron-doped diamond (AuNPs-BDD) electrodes, which were prepared with a self-assembly deposition of AuNPs at amine-terminated boron-doped diamond, were examined for voltammetric detection of neuraminidase (NA). The detection method was performed based on the difference of electrochemical responses of zanamivir at gold surface before and after the reaction with NA in phosphate buffer solution (PBS, pH 5.5). A linear calibration curve for zanamivir in 0.1 M PBS in the absence of NA was achieved in the concentration range of 1 × 10(-6) to 1 × 10(-5) M (R(2) = 0.99) with an estimated limit of detection (LOD) of 2.29 × 10(-6) M. Furthermore, using its reaction with 1.00 × 10(-5) M zanamivir, a linear calibration curve of NA can be obtained in the concentration range of 0-12 mU (R(2) = 0.99) with an estimated LOD of 0.12 mU. High reproducibility was shown with a relative standard deviation (RSD) of 1.14% (n = 30). These performances could be maintained when the detection was performed in mucin matrix. Comparison performed using gold-modified BDD (Au-BDD) electrodes suggested that the good performance of the detection method is due to the stability of the gold particles position at the BDD surface.
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Sciences, University of Indonesia, Kampus UI Depok, Jakarta 16424, Indonesia.
authors.forename: Wulan T
authors.initials: WT
authors.lastname: Wahyuni
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Sciences, University of Indonesia, Kampus UI Depok, Jakarta 16424, Indonesia. Electronic address: ivandini.tri@ui.ac.id.
authors.forename: Tribidasari A
authors.initials: TA
authors.lastname: Ivandini
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Sciences, University of Indonesia, Kampus UI Depok, Jakarta 16424, Indonesia.
authors.forename: Endang
authors.initials: E
authors.lastname: Saepudin
authors.affiliation: Department of Chemistry, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan; JST ACCEL, Yokohama 223-8522, Japan. Electronic address: einaga@chem.keio.ac.jp.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Einaga
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D001895:Boron / Q000737:chemistry*; D003016:Clostridium perfringens / Q000201:enzymology*; D018130:Diamond / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004791:Enzyme Inhibitors / Q000032:analysis / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009439:Neuraminidase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D053243:Zanamivir / Q000032:analysis* / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D006046:Gold; D018130:Diamond; D009439:Neuraminidase; D053243:Zanamivir; D001895:Boron
keywords: Electroanalysis; Enzymatic inhibition; Gold nanoparticle; Neuraminidase; Zanamivir