title: The suppressive effect of dietary coenzyme Q10on mitochondrial reactive oxygen species production and oxidative stress in chickens exposed to heat stress.
abstract: This study was conducted to determine if dietary supplementation with coenzyme Q10(CoQ10), which can act as a potent antioxidant and is an obligatory cofactor of mitochondrial uncoupling protein, suppresses the heat stress (HS)-induced overproduction of mitochondrial reactive oxygen species (ROS) and oxidative damage in the skeletal muscle of birds. The carbonyl protein content of skeletal muscle was significantly higher in birds exposed to HS treatment (34°C, 12 h) than in thermoneutral birds (25°C). This increase was suppressed by CoQ10supplementation (40 mg/kg diet). Succinate-supported mitochondrial ROS production was increased by HS treatment, and this increase was also suppressed by CoQ10supplementation. In contrast, CoQ10supplementation did not affect the HS-induced decrease in mitochondrial proton leak. The mitochondrial membrane potential (ΔΨ), to which HS-induced ROS production was previously shown to be sensitive, tended to be increased by HS treatment, but this rise in ΔΨ was not affected by CoQ10supplementation. Taken together, these results suggest that dietary CoQ10supplementation attenuates HS-induced oxidative damage to skeletal muscle, by preventing the overproduction of succinate-supported mitochondrial ROS in a manner that is independent of ΔΨ. © 2015 Japanese Society of Animal Science.
authors.affiliation: Animal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Kikusato
authors.affiliation: Animal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Kasumi
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Animal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Mikami
authors.affiliation: Animal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Mujahid
authors.affiliation: Animal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. toyomizu@bios.tohoku.ac.jp.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Toyomizu
mesh_terms: D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D002645:Chickens; D003864:Depression, Chemical; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D006358:Hot Temperature / Q000009:adverse effects*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects* / Q000502:physiology*; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D014815:Vitamins / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D014815:Vitamins; D014451:Ubiquinone; C024989:coenzyme Q10
keywords: basal proton leak; carbonyl protein content; membrane potential; reverse electron flow