title: Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013-2014.
abstract: Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013-2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jindai
authors.initials: J
authors.lastname: Fan
authors.affiliation: Guangdong Wen's Food Co. Ltd., Xinxing 527400, China.
authors.forename: Xiduo
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Guangdong Wen's Food Co. Ltd., Xinxing 527400, China.
authors.forename: Guanqun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Guangdong Wen's Food Co. Ltd., Xinxing 527400, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Niu
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Xie
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks / Q000662:veterinary*; D011558:Herpesvirus 1, Suid / Q000145:classification / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D011557:Pseudorabies / Q000453:epidemiology* / Q000821:virology; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: molecular characterization; phylogenetic analysis; pseudorabies virus