title: MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella.
abstract: MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs in antibacterial defence in fish has not been fully determined. Here, we identified that nine miRNAs are differentially expressed in kidney between susceptible and resistant grass carp strains. Analysis of spatial and temporal miRNA expression patterns suggests that cid-miRn-115 and miR-142a-3p are potential regulators of anti-bacterial activity. Overexpressing of cid-miRn-115 and miR-142a-3p results in a visible change in Ctenopharyngodon idella kidney (CIK) cells immune effector activity. Bioinformatics analysis and overexpressing assay shows that cid-miRn-115 and miR-142a-3p directly regulate tlr5 expression. cid-miRn-115 and miR-142a-3p overexpressing leads to a significant decrease in tlr5 expression in CIK, thereby repressing its downstream genes, such as il-1β, il-8 and tnf-α. These findings provide a novel insight into the determination of anti-bacterial compounds in grass carp.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xu
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors.forename: Yu-Bang
authors.initials: YB
authors.lastname: Shen
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Fu
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Yu
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Huang
authors.affiliation: National Pathogen Collection Center for Aquatic Animals, College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, PR China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Lu
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.|E-Institute of Shanghai Universities, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, PR China.
authors.forename: Jia-Le
authors.initials: JL
authors.lastname: Li
mesh_terms: D000818:Animals; D001665:Binding Sites; D002347:Carps / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D007109:Immunity / Q000235:genetics; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009690:Nucleic Acid Conformation; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D051198:Toll-Like Receptor 5 / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D051198:Toll-Like Receptor 5