title: The Activity of Cotinus coggygria Scop. Leaves on Staphylococcus aureus Strains in Planktonic and Biofilm Growth Forms.
abstract: The purpose of this study was to detect the effectiveness of Cotinus coggygria Scop. leaves methanol extract against planktonic and biofilm growth forms of Staphylococcus aureus. The antimicrobial activity was determined by the broth microdilution test. Minimal inhibitory concentrations and minimal bactericidal concentrations were detected against two collection and ten clinical S. aureus strains. Anti-biofilm activity of the tested extract was detected using 24 h bacterial biofilm on the surface of microtiter plate wells. The biofilm inhibitory activity was evaluated visually after 24 h interaction of extract with biofilm, and the eradicating activity by a regrowth method. The tested extract showed bactericidal activity against all S. aureus strains (methicillin susceptible or methicillin resistant) in concentrations ranging from 0.313 to 0.625 mg·mL(-1). Biofilm inhibitory concentrations were 10-times higher and biofilm eradicating concentrations 100-times higher (8 and 32 mg·mL(-1), respectively). The phytochemical analysis of C. coggygria leaves 60% methanol extract performed by LC-DAD-MS/MS revealed quercetin rhamnoside, methyl gallate, and methyl trigallate as main constituents. Results of our study indicate that C. coggygria, rich in tannins and flavonoids, seems to be a prospective topical antibacterial agent with anti-biofilm activity.
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia. rendekova18@uniba.sk.
authors.forename: Katarína
authors.initials: K
authors.lastname: Rendeková
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia. fialova@fpharm.uniba.sk.
authors.forename: Silvia
authors.initials: S
authors.lastname: Fialová
authors.affiliation: Department of Pharmaceutical Analysis and Nuclear Pharmacy, Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia. veizerova@fpharm.uniba.sk.
authors.forename: Lucia
authors.initials: L
authors.lastname: Jánošová
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia. mucaji@fpharm.uniba.sk.
authors.forename: Pavel
authors.initials: P
authors.lastname: Mučaji
authors.affiliation: Institute of Microbiology of the Medical Faculty and the University Hospital in Bratislava, Comenius University in Bratislava, Sasinkova 4, 811 08 Bratislava, Slovakia. livia.slobodnikova@fmed.uniba.sk.
authors.forename: Lívia
authors.initials: L
authors.lastname: Slobodníková
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D000432:Methanol / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010933:Plankton / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D000432:Methanol
keywords: Cotinus coggygria; Staphylococcus aureus; anti-biofilm activity; antibacterial activity; fingerprint; leaves