title: Bacterioplankton community analysis in tilapia ponds by Illumina high-throughput sequencing.
abstract: The changes of microbial community in aquaculture systems under the effects of stocking densities and seasonality were investigated in tilapia ponds. Total DNAs were extracted from the water samples, 16S rRNA gene was amplified and the bacterial community analyzed by Illumina high-throughput sequencing obtaining 3486 OTUs, from a total read of 715,842 sequences. Basing on the analysis of bacterial compositions, richness, diversity, bacterial 16S rRNA gene abundance, water sample comparisons and existence of specific bacterial taxa within three fish ponds in a 4 months period, the study conclusively observed that the dominant phylum in all water samples were similar, and they included; Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Planctomycetes and Chlorobi, distributed in different proportions in the different months and ponds. The seasonal changes had a more pronounced effect on the bacterioplankton community than the stocking densities; however some differences between the ponds were more likely caused by feed coefficient than by stocking densities. At the same time, most bacterial communities were affected by the nutrient input except phylum Cyanobacteria that was also affected by the feed control of tilapia.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214182, Jiangsu Province, China.|Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China.
authors.forename: Li Min
authors.initials: LM
authors.lastname: Fan
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214182, Jiangsu Province, China.
authors.forename: Kamira
authors.initials: K
authors.lastname: Barry
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China.
authors.forename: Geng Dong
authors.initials: GD
authors.lastname: Hu
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China.
authors.forename: Shun long
authors.initials: Sl
authors.lastname: Meng
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Song
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214182, Jiangsu Province, China. chenjz@ffrc.cn.|Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China. chenjz@ffrc.cn.
authors.forename: Jia Zhang
authors.initials: JZ
authors.lastname: Chen
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214182, Jiangsu Province, China. xup@ffrc.cn.|Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China. xup@ffrc.cn.
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D044822:Biodiversity; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D010933:Plankton / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012621:Seasons; D017422:Sequence Analysis, DNA; D017210:Tilapia / Q000382:microbiology*; D014867:Water / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014867:Water
keywords: Bacterioplankton community; Illumina high throughput sequencing; Tilapia ponds