title: Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
abstract: Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues. While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling. Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function. Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis. These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases. Additionally, galt2gal5 as well as sos5 and fei2 showed reduced seed mucilage adherence. Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway. Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Debarati
authors.initials: D
authors.lastname: Basu
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Lu
authors.initials: L
authors.lastname: Tian
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Tayler
authors.initials: T
authors.lastname: Debrosse
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Emily
authors.initials: E
authors.lastname: Poirier
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Kirk
authors.initials: K
authors.lastname: Emch
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Hayley
authors.initials: H
authors.lastname: Herock
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Andrew
authors.initials: A
authors.lastname: Travers
authors.affiliation: Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701-2979, United States of America.
authors.forename: Allan M
authors.initials: AM
authors.lastname: Showalter
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D008031:Lignin / Q000378:metabolism; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology; D062087:Plant Mucilage / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D015815:Cell Adhesion Molecules; D010937:Plant Growth Regulators; D062087:Plant Mucilage; C473610:SOS5 protein, Arabidopsis; D012965:Sodium Chloride; D013395:Sucrose; D002482:Cellulose; D008031:Lignin; D005700:Galactosyltransferases; D020794:Receptor Protein-Tyrosine Kinases; C000609096:FEI1 protein, Arabidopsis; C000609097:FEI2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases