title: Resolvin D1 and E1 promote resolution of inflammation in microglial cells in vitro.
abstract: Sustained inflammation in the brain together with microglia activation can lead to neuronal damage. Hence limiting brain inflammation and activation of microglia is a real therapeutic strategy for inflammatory disease. Resolvin D1 (RvD1) and resolvin E1 (RvE1) derived from n-3 long chain polyunsaturated fatty acids are promising therapeutic compounds since they actively turn off the systemic inflammatory response. We thus evaluated the anti-inflammatory activities of RvD1 and RvE1 in microglia cells in vitro. BV2 cells were pre-incubated with RvD1 or RvE1 before lipopolysaccharide (LPS) treatment. RvD1 and RvE1 both decreased LPS-induced proinflammatory cytokines (TNF-α, IL-6 and IL-1β) gene expression, suggesting their proresolutive activity in microglia. However, the mechanisms involved are distinct as RvE1 regulates NFκB signaling pathway and RvD1 regulates miRNAs expression. Overall, our findings support that pro-resolving lipids are involved in the resolution of brain inflammation and can be considered as promising therapeutic agents for brain inflammation.
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; ITERG, Institut des corps gras, 33600 Pessac, France.
authors.forename: C
authors.initials: C
authors.lastname: Rey
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France.
authors.forename: A
authors.initials: A
authors.lastname: Nadjar
authors.affiliation: ITERG, Institut des corps gras, 33600 Pessac, France.
authors.forename: B
authors.initials: B
authors.lastname: Buaud
authors.affiliation: ITERG, Institut des corps gras, 33600 Pessac, France.
authors.forename: C
authors.initials: C
authors.lastname: Vaysse
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France.
authors.forename: A
authors.initials: A
authors.lastname: Aubert
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France.
authors.forename: V
authors.initials: V
authors.lastname: Pallet
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France.
authors.forename: S
authors.initials: S
authors.lastname: Layé
authors.affiliation: INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France; Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France. Electronic address: corinne.joffre@bordeaux.inra.fr.
authors.forename: C
authors.initials: C
authors.lastname: Joffre
mesh_terms: D000818:Animals; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy*; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D017628:Microglia / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects*
chemical_list: D015850:Interleukin-6; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; C518399:resolvin D1; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; C499823:5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid
keywords: BV2; DHA; EPA; Microglial cells; Neuroinflammation; RvD1; RvE1; miRNA; n-3 PUFA