title: Complex analysis of the p53 tumor suppressor in lung carcinoma.
abstract: Lung cancer is the leading cause of cancer-related deaths worldwide. The p53 tumor suppressor is a transcription factor controlling expression of its target genes in response to various stress stimuli. Mutations of the TP53 gene occur very frequently in lung carcinomas and they play an important role in both oncogenic transformation of lung epithelial cells and lung carcinoma progression. We determined the TP53 status in 42 samples of squamous cell lung carcinoma (SQCC) and 56 samples of lung adenocarcinoma (AC) by the functional analysis FASAY and its variant called split assay. Altogether, we detected 64 TP53 mutations in 63 patients and analyzed them by cDNA and gDNA sequencing. The TP53 mutations were found in 76.2% (32/42) of SQCC cases, and 55.4% (31/56) of ACs. Immunoblotting revealed the p53 protein accumulation in 18 samples (42.9%) among SQCC cases and 19 samples (33.9%) among AC cases. Using fluorescence in situ hybridization we detected loss of the TP53-specific 17p13.3 locus in 23 from 41 analyzed SQCC samples (56.1%) and in 20 from 54 analyzed AC samples (37.0%). We did not find any statistically significant differences in overall and disease-free survival in relation to TP53 status.
authors.affiliation: Department of Pathology, University Hospital, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Smardova
authors.affiliation: Department of Pathology, University Hospital, Brno, Czech Republic.
authors.forename: Kvetoslava
authors.initials: K
authors.lastname: Liskova
authors.affiliation: Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Ravcukova
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Malcikova
authors.affiliation: Department of Pathology, University Hospital, Brno, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Hausnerova
authors.affiliation: Department of Pathology, University Hospital, Brno, Czech Republic.
authors.forename: Miluse
authors.initials: M
authors.lastname: Svitakova
authors.affiliation: Department of Pathology, University Hospital, Brno, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Hrabalkova
authors.affiliation: Department of Pathology, University Hospital, Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Zlamalikova
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Stano-Kozubik
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
authors.forename: Ivona
authors.initials: I
authors.lastname: Blahakova
authors.affiliation: Department of Respiratory Diseases and TB, University Hospital, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Speldova
authors.affiliation: Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Jarkovsky
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Smarda
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53