title: Determination of Sequences Required for Human Endogenous Retrovirus K Transduction and Its Recognition by Foreign Retroviral Virions.
abstract: Sequences necessary for transduction of human endogenous retrovirus (HERV)-Kcon, a consensus of the HERV-K(HML-2) family, were analyzed and found to reside in the leader/gag region. They act in an orientation-dependent way and consist of at least two sites working together. Having defined these sequences, we exploited this information to produce a simple system to investigate to what extent virions of HERV-Kcon, murine leukemia virus, and HIV-1 have the ability to transduce each other's genomes, leading to potential contamination of gene therapy vectors.
authors.affiliation: Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom.
authors.forename: Otto
authors.initials: O
authors.lastname: Erlwein
authors.affiliation: Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Sweeney
authors.affiliation: Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom.
authors.forename: Raffaele
authors.initials: R
authors.lastname: de Leon
authors.affiliation: Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom.
authors.forename: Gillian
authors.initials: G
authors.lastname: Wills
authors.affiliation: Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Robinson
authors.affiliation: Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom mmcclure@imperial.ac.uk.
authors.forename: Myra O
authors.initials: MO
authors.lastname: McClure
mesh_terms: D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D020077:Endogenous Retroviruses / Q000235:genetics*; D015683:Gene Products, gag / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D004279:DNA, Viral; D015683:Gene Products, gag