title: Identification and Evaluation of Antiepileptic Activity of C21 Steroidal Glycosides from the Roots of Cynanchum wilfordii.
abstract: Nine new C21 steroidal glycosides, named cynawilfosides A-I (1-9), along with 12 known compounds were isolated from the roots of Cynanchum wilfordii. The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. The five major components, cynawilfoside A (1), cynauricoside A (11), wilfoside C1N (16), wilfoside K1N (17), and cyanoauriculoside G (18), exhibited significant protection activity in a maximal electroshock (MES)-induced mouse seizure model with ED50 values of 48.5, 95.3, 124.1, 72.3, and 88.1 mg/kg, respectively.
authors.affiliation: Department of Natural Product Chemistry and ‡Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203, People's Republic of China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Li
authors.affiliation: Department of Natural Product Chemistry and ‡Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203, People's Republic of China.
authors.forename: Zhao-Bing
authors.initials: ZB
authors.lastname: Gao
authors.affiliation: Department of Natural Product Chemistry and ‡Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203, People's Republic of China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031105:Cynanchum / Q000737:chemistry*; D004195:Disease Models, Animal; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012503:Saponins; D012640:Seizures
chemical_list: D000927:Anticonvulsants; D006027:Glycosides; D011278:Pregnanes; D012503:Saponins; C535062:wilfoside C1N