title: [Regulation of UC-MSC on Immune Inflammatory Thrombophilia in MRL/lpr Mice].
abstract: OBJECTIVE
  To study the immune repair effect of umbilical cord mesenchymal stem cells (UC-MSC) on inflammatory disorders and thrombophilia state of MRL/lpr mice by detecting the expression change of peripheral blood CD4(+) CD25(+) T cells and the levels of plasma inflammatory cytokines TNF-α, IL-6 and thrombosis indicators TF, FIB.
  
  
  METHODS
  Twenty five MRL/lpr mice were divided into control (C) group, UC-MSC one time treatment (UT1) group and UC-MSC three time treatments (UT3) group. UC-MSC cell suspension was injecled via tail vein and these mice were feeded in SPF environment. The blood samples were taken from the mice every 2 weeks after 16(th) week. FCM was used to detect the expression of CD4(+) CD25(+) T cells in peripheral blood, ELISA assay was used to detect the levels of inflammatory cytokines TNF-α, IL-6 and thrombosis indicators TF, FIB.
  
  
  RESULTS
  The expression of peripheral blood CD4(+) CD25(+) T cells in treatment groups increased during 16(th) to 18(th) week, dropped and tended to be stable since 20(th) week, and lower than those in control group. The levels of plasma TNF-α and IL-6 in treatment group decreased since 16(th) week and significantly lower than those in control group (P < 0.05). The levels of plasma TF and FIB in treatment group decreased since 16(th) week. The level of plasma TF in treatment group was significantly lower than those in control group (P < 0.05) since 18(th) week.
  
  
  CONCLUSIONS
  UC-MSC can repair the immune inflammatory microenvironment disorders of MRL/lpr mice through its immunomodulatory effect. UC-MSC contribute to repair of immune inflammatory thrombophilia of MRL/lpr mice.
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China.
authors.forename: Xin-Zhen
authors.initials: XZ
authors.lastname: Cai
authors.affiliation: Subei People's Hospital of Jiangsu Province, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China.
authors.forename: Zou
authors.initials: Z
authors.lastname: Li
authors.affiliation: Subei People's Hospital of Jiangsu Province, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Shen
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China. E-mail: linhmd@163.com.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
authors.affiliation: Subei People's Hospital of Jiangsu Province, Yangzhou 225001, Jiangsu Province, China. E-mail: maolujiu918@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D015850:Interleukin-6; D059630:Mesenchymal Stem Cells; D051379:Mice; D019463:Mice, Inbred MRL lpr; D013601:T-Lymphocytes; D019851:Thrombophilia; D014409:Tumor Necrosis Factor-alpha; D014470:Umbilical Cord
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha