title: Fumigaclavine I, a new alkaloid isolated from endophyte Aspergillus terreus.
abstract: The present study was designed to isolate and purify chemical constituents from solid culture of endophyte Aspergillus terreus LQ, using silica gel column chromatography, gel filtration with Sephadex LH-20, and HPLC. Fumigaclavine I (1), a new alkaloid, was obtained, along with seven known compounds, including fumigaclavine C (2), rhizoctonic acid (3), monomethylsulochrin (4), chaetominine (5), spirotryprostatin A (6), asperfumoid (7), and lumichrome (8). The structure of compound 1 was elucidated by various spectroscopic analyses (UV, MS, 1D and 2D NMR). The in vitro cytotoxicity of compound 1 was determined by MTT assay in human hepatocarcinoma cell line SMMC-7721, showing weaker cytotoxicity, compared with cisplatin, a clinically used cancer chemotherapeutic agent.
authors.affiliation: Medical College, Yangzhou University, Yangzhou 225001, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: shenli@yzu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.affiliation: Medical College, Yangzhou University, Yangzhou 225001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Medical College, Yangzhou University, Yangzhou 225001, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Medical College, Yangzhou University, Yangzhou 225001, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Li
authors.affiliation: Medical College, Yangzhou University, Yangzhou 225001, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China.
authors.forename: Ju-Qun
authors.initials: JQ
authors.lastname: Xi
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China.
authors.forename: Gui-Mei
authors.initials: GM
authors.lastname: Kong
authors.affiliation: Institute of Functional Biomolecules, Nanjing University, Nanjing 210046, China.
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Song
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification; D001230:Aspergillus / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D060026:Endophytes / Q000737:chemistry* / Q000302:isolation & purification; D004876:Ergot Alkaloids / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012275:Oryza / Q000382:microbiology*
chemical_list: D000970:Antineoplastic Agents; D004876:Ergot Alkaloids; C000611397:fumigaclavine I
keywords: Aspergillus terreus; Chemical constituent; Endophyte; Ergot alkaloid