title: Sulfide-driven autotrophic denitrification significantly reduces N2O emissions.
abstract: The Sulfate reduction-Autotrophic denitrification-Nitrification Integrated (SANI) process build on anaerobic carbon conversion through biological sulfate reduction and autotrophic denitrification by using the sulfide byproduct from the previous reaction. This study confirmed extra decreases in N2O emissions from the sulfide-driven autotrophic denitrification by investigating N2O reduction, accumulation, and emission in the presence of different sulfide/nitrate (S/N) mass ratios at pH 7 in a long-term laboratory-scale granular sludge autotrophic denitrification reactor. The N2O reduction rate was linearly proportional to the sulfide concentration, which confirmed that no sulfide inhibition of N2O reductase occurred. At S/N = 5.0 g-S/g-N, this rate resulted by sulfide-driven autotrophic denitrifying granular sludge (average granule size = 701 μm) was 27.7 mg-N/g-VSS/h (i.e., 2 and 4 times greater than those at 2.5 and 0.8 g-S/g-N, respectively). Sulfide actually stimulates rather than inhibits N2O reduction no matter what granule size of sulfide-driven autotrophic denitrifying sludge engaged. The accumulations of N2O, nitrite and free nitrous acid (FNA) with average granule size 701 μm of sulfide-driven autotrophic denitrifying granular sludge engaged at S/N = 5.0 g-S/g-N were 4.7%, 11.4% and 4.2% relative to those at 3.0 g-S/g-N, respectively. The accumulation of FNA can inhibit N2O reduction and increase N2O accumulation during sulfide-driven autotrophic denitrification. In addition, the N2O gas emission level from the reactor significantly increased from 14.1 ± 0.5 ppmv (0.002% of the N load) to 3707.4 ± 36.7 ppmv (0.405% of the N load) as the S/N mass ratio in the influent decreased from 2.1 to 1.4 g-S/g-N over the course of the 120-day continuous monitoring period. Sulfide-driven autotrophic denitrification may significantly reduce greenhouse gas emissions from biological nutrient removal when sulfur conversion processes are applied.
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Civil Engineering, Guangzhou University, Guangzhou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, China. Electronic address: lvhui3@mail.sysu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Xiaping Solid Waste Landfill Site, Shenzhen, China.
authors.forename: Liao
authors.initials: L
authors.lastname: Meng
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, China; Department of Civil & Environmental Engineering, HKUST, Clear Water Bay, Hong Kong, China.
authors.forename: Guang-Hao
authors.initials: GH
authors.lastname: Chen
mesh_terms: D052818:Autotrophic Processes; D019149:Bioreactors; D058440:Denitrification; D058465:Nitrification; D009609:Nitrous Oxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D012722:Sewage; D013440:Sulfides / Q000737:chemistry*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D012722:Sewage; D013440:Sulfides; D009609:Nitrous Oxide
keywords: Autotrophic denitrification; Nitrous oxide; SANI process; Sulfide