title: Functional Analysis of Esterase TCE2 Gene from Tetranychus cinnabarinus (Boisduval) involved in Acaricide Resistance.
abstract: The carmine spider mite, Tetranychus cinnabarinus is an important pest of crops and vegetables worldwide, and it has the ability to develop resistance against acaricides rapidly. Our previous study identified an esterase gene (designated TCE2) over-expressed in resistant mites. To investigate this gene's function in resistance, the expression levels of TCE2 in susceptible, abamectin-, fenpropathrin-, and cyflumetofen-resistant strains were knocked down (65.02%, 63.14%, 57.82%, and 63.99%, respectively) via RNA interference. The bioassay data showed that the resistant levels to three acaricides were significantly decreased after the down-regulation of TCE2, indicating a correlation between the expression of TCE2 and the acaricide-resistance in T. cinnabarinus. TCE2 gene was then re-engineered for heterologous expression in Escherichia coli. The recombinant TCE2 exhibited α-naphthyl acetate activity (483.3 ± 71.8 nmol/mg pro. min(-1)), and the activity of this enzyme could be inhibited by abamectin, fenpropathrin, and cyflumetofen, respectively. HPLC and GC results showed that 10 μg of the recombinant TCE2 could effectively decompose 21.23% fenpropathrin and 49.70% cyflumetofen within 2 hours. This is the first report of a successful heterologous expression of an esterase gene from mites. This study provides direct evidence that TCE2 is a functional gene involved in acaricide resistance in T. cinnabarinus.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wei
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Xiangzun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Guangmao
authors.initials: G
authors.lastname: Shen
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Biology, Abilene Christian University, Abilene, Texas, U.S.A.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: He
mesh_terms: D056810:Acaricides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D004950:Esterases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D007700:Kinetics; D007928:Lethal Dose 50; D011422:Propionates / Q000737:chemistry / Q000494:pharmacology*; D011722:Pyrethrins / Q000737:chemistry / Q000494:pharmacology*; D034622:RNA Interference; D040102:Tetranychidae
chemical_list: D056810:Acaricides; D060829:Arthropod Proteins; D011422:Propionates; D011722:Pyrethrins; C044267:fenpropathrin; D004950:Esterases; C572153:cyflumetofen