title: [Metagenomic analysis of taxonomic and functional changes in gut microbiota of patients with alcoholic dependence syndrome].
abstract: Here we present the first metagenomic study of gut microbiota in patients with alcohol dependence syndrome (ADS) performed in the whole-genome ("shotgun") format. Taxonomic analysis highlighted changes in community "drivers" abundance previously associated with inflammatory processes (including increase in Ruminococcus gnavus and torques, as well as decrease in Faecalibacterium and Akkermansia). Microbiota of alcoholics manifested presence of specific opportunistic pathogens rarely detected in healthy control subjects of the world. Differential analysis of metabolic potential basing on changes in KEGG Orthology groups abundance revealed increase in pathways associated with response to oxidative stress. Analysis of two specific gene groups--alcohol metabolism and virulence factors--also showed increase in comparison with the control groups. We suggest that gut microbiota distinct in alcoholics by both taxonomic and functional composition plays role in modulating the effect of alcohol on host organism.
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia.
authors.forename: V B
authors.initials: VB
authors.lastname: Dubinkina
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia; Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Tyakht
authors.affiliation: Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Ilina
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia; Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Ischenko
authors.affiliation: Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: B A
authors.initials: BA
authors.lastname: Kovarsky
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia.
authors.forename: K S
authors.initials: KS
authors.lastname: Yarygin
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia; Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Pavlenko
authors.affiliation: Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Popenko
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia; Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: D G
authors.initials: DG
authors.lastname: Alexeev
authors.affiliation: Saint-Petersburg Bekhterev Psychoneurological Research Institute, Saint-Petersburg, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Taraskina
authors.affiliation: Saint-Petersburg Bekhterev Psychoneurological Research Institute, Saint-Petersburg, Russia.
authors.forename: R F
authors.initials: RF
authors.lastname: Nasyrova
authors.affiliation: Saint-Petersburg Bekhterev Psychoneurological Research Institute, Saint-Petersburg, Russia.
authors.forename: E M
authors.initials: EM
authors.lastname: Krupitski
authors.affiliation: Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: L O
authors.initials: LO
authors.lastname: Skorodumova
authors.affiliation: Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: A K
authors.initials: AK
authors.lastname: Larin
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia; Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Kostryukova
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia; Scientific Research Institute of Physico-Chemical Medicine, Department of Molecular Biology and Genetics, Moscow, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Govorun
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000378:metabolism / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D018384:Oxidative Stress; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D000431:Ethanol
keywords: alcohol dependence syndrome; gut microbiota; metabolic potential; metagenome; microbial alcohol metabolism; virulence factors