title: Overexpression and lack of copy number variation in the BMI-1 gene in human glioma.
abstract: Malignant gliomas are neoplasms of the brain that are associated with a poor prognosis. The B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) gene is one of the major cancer stem cell factors responsible for treatment failure in glioma. In the present study, the DNA-RNA-protein alterations in the BMI-1 gene were assessed in 50 glioma samples. Copy number variations in the BMI-1 gene were analyzed using SYBR® Green quantitative polymerase chain reaction. Gene expression analysis was performed using a Taqman assay and protein quantitation was performed using western blotting. A comparative Ct analysis showed the absence of copy number variations in all glioma samples. BMI-1 mRNA expression was found to be overexpressed in 36 out of 50 samples (72.0%), and 37 out of 50 samples showed overexpression (74.0%) of BMI-1 protein; this was statistically significant when compared with non-glioma tissues. It was observed that the protein and RNA expression in glioma were concordant. In this study on the BMI-1 gene, transcription and translation in glioma were observed and BMI-1 overexpression was found to be a common phenomenon.
authors.affiliation: Department of Human Genetics, National Institute of Mental Health and Neurosciences, Bangalore, Karnataka 560029, India.
authors.forename: Sibin
authors.initials: S
authors.lastname: Madathan Kandy
authors.affiliation: Department of Neurosurgery, National Institute of Mental Health and Neurosciences, Bangalore, Karnataka 560029, India.
authors.forename: Dhananjaya
authors.initials: D
authors.lastname: Ishwara Bhat
authors.affiliation: Department of Human Genetics, National Institute of Mental Health and Neurosciences, Bangalore, Karnataka 560029, India.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Choppavarapu
authors.affiliation: Department of Human Genetics, National Institute of Mental Health and Neurosciences, Bangalore, Karnataka 560029, India.
authors.forename: Arati
authors.initials: A
authors.lastname: Suvatha
authors.affiliation: Department of Human Genetics, National Institute of Mental Health and Neurosciences, Bangalore, Karnataka 560029, India.
authors.forename: Chetan
authors.initials: C
authors.lastname: Ghati Kasturirangan
keywords: BMI-1; copy number; glioma; overexpression