title: Evidence of vertical transmission and co-circulation of chikungunya and dengue viruses in field populations of Aedes aegypti (L.) from Guerrero, Mexico.
abstract: BACKGROUND
  We report results of the entomo-virological surveillance system in Aedes aegypti local populations performed by the Ministry of Health of Guerrero.
  
  
  METHODS
  Indoor-adult Ae. aegypti collected at Acapulco, Zihuatanejo, Coyuca de Benitez and Atoyac de Alvarez (dry season, 2015) were processed for dengue virus (DENV) and chikungunya virus (CHIKV) using RT-PCR.
  
  
  RESULTS
  We identified different seroptypes of DENV (2, 3 and 4), CHIKV and their co-circulation in field-caught mosquitoes across a significant geographic area. Pools of males were positive for CHIKV and DENV 3 and 4 suggesting vertical transmission.
  
  
  CONCLUSIONS
  Entomo-virological surveillance in Guerrero has identified early circulation of CHIKV and DENV and provided a trigger for timely and focalized vector control actions.
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Felipe
authors.initials: F
authors.lastname: Dzul-Manzanilla
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Norma E
authors.initials: NE
authors.lastname: Martínez
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Maximina
authors.initials: M
authors.lastname: Cruz-Nolasco
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Cipriano
authors.initials: C
authors.lastname: Gutiérrez-Castro
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Leonardo
authors.initials: L
authors.lastname: López-Damián
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Jesús
authors.initials: J
authors.lastname: Ibarra-López
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Andres
authors.initials: A
authors.lastname: Martini-Jaimes
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Wilbert
authors.initials: W
authors.lastname: Bibiano-Marín
authors.affiliation: Servicios Estatales de Salud de Guerrero, Avenida Ruffo Figueroa No. 6, Col. Burócratas, Chilpancingo, Guerrero, C.P. 39090, México.
authors.forename: Citlalli
authors.initials: C
authors.lastname: Tornez-Benitez
authors.affiliation: Department of Environmental Sciences, Emory University, Atlanta, GA, 30322, USA.
authors.forename: Gonzalo M
authors.initials: GM
authors.lastname: Vazquez-Prokopec
authors.affiliation: Unidad Colaborativa de Bioensayos Entomológicos, Departamento de Zoología, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Km. 15.5 Carr. Mérida-Xmatkuil s.n., Mérida, Yucatán, C.P. 97315, México msaide@correo.uady.mx pablo_manrique2000@hotmail.com.
authors.forename: Pablo
authors.initials: P
authors.lastname: Manrique-Saide
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D065632:Chikungunya Fever / Q000453:epidemiology / Q000235:genetics / Q000635:transmission*; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D003430:Cross-Sectional Studies; D003715:Dengue / Q000453:epidemiology / Q000235:genetics / Q000635:transmission*; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000706:statistics & numerical data*; D007303:Insect Vectors; D008800:Mexico / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction; D018571:Sentinel Surveillance
keywords: Aedes aegypti; Chikungunya; Dengue; Mexico; Surveillance