title: MicroRNA-485-5p suppresses cell proliferation and invasion in hepatocellular carcinoma by targeting stanniocalcin 2.
abstract: Increasing evidences indicate that dys-regulation of MicroRNAs contributes to hepatocellular carcinoma. However, the roles of miR-485-5p in HCC are still largely unexplored. In the present study, our quantitative real-time PCR analysis found that miR-485-5p was significantly down-regulated in 50 pairs of human HCC tissues. Moreover, the reduced expression of miR-485-5p was significantly correlated with larger tumor size and more tumor number in patients with HCC. In vitro studies further showed that overexpression of miR-485-5p mimics could inhibit, while its antisense oligos promote cell proliferation and invasion. Results from the dual-luciferase reporter gene assays and western blot further showed that stanniocalcin 2 was a direct target of miR-485-5p. Therefore, our data suggest a novel role for miR-485-5p in the regulation of HCC progression.
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, Henan Province, China.
authors.forename: Guo-Xiao
authors.initials: GX
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, Henan Province, China.
authors.forename: Quan-Ying
authors.initials: QY
authors.lastname: Li
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, Henan Province, China.
authors.forename: Wan-Li
authors.initials: WL
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, Henan Province, China.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Shi
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, Henan Province, China.
authors.forename: Xue-Qun
authors.initials: XQ
authors.lastname: Ren
mesh_terms: D063646:Carcinogenesis; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000601:surgery; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology* / Q000601:surgery; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged
chemical_list: D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C556501:MIRN485 microRNA, human; D035683:MicroRNAs; C114997:STC2 protein, human
keywords: Hepatocellular carcinoma; MicroRNA; miR-485-5p; stanniocalcin 2