title: Enolase of Streptococcus Suis Serotype 2 Enhances Blood-Brain Barrier Permeability by Inducing IL-8 Release.
abstract: Streptococcus suis serotype 2 (SS2) is an emerging zoonosis, and meningitis is the most frequent clinical manifestation, but mechanism of its virulent factor, enolase (Eno), is unknown in meningitis. In this study, Eno was inducibly expressed and added to an in vitro Transwell co-culture model of the blood-brain barrier (BBB) consisted of porcine brain microvascular endothelial cells (PBMECs) and astrocytes (ACs), the results showed that Eno induces a significant increase in BBB permeability and promotes the release of IL-8 et al. cytokines. Furthermore, IL-8 could significantly destroy the integrity of the BBB model in vitro. In mice models administered Eno for 24 h, Eno could significantly promote Evans blue (EB) moving from the blood to the brain and significantly increased the serum and brain levels of IL-8, as detected by ELISA. While G31P (IL-8 receptor antagonist) significantly decreased the concentration of EB in the brains of mice injected with Eno. The present study demonstrated that SS2 Eno may play an important role in disrupting BBB integrity by prompting IL-8 release.
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Changchun University of Chinese Medicine, Changchun, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Gu
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Chongtao
authors.initials: C
authors.lastname: Du
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 130062, China. leiliancheng@163.com.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002199:Capillary Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D016209:Interleukin-8 / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000494:pharmacology; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000494:pharmacology*; D017011:Streptococcus suis / Q000472:pathogenicity*; D013552:Swine; D037521:Virulence Factors / Q000494:pharmacology
chemical_list: C457773:CXCL8(3-73)K11R,G31P, bovine; D016209:Interleukin-8; D010446:Peptide Fragments; D037521:Virulence Factors; D010751:Phosphopyruvate Hydratase
keywords: blood–brain barrier; enolase; meningitis; streptococcus suis serotype 2