title: GMP-grade platelet lysate enhances proliferation and migration of tenon fibroblasts.
abstract: Tenon's fibroblasts (TFs), widely employed as in vitro model for many ophthalmological studies, are routinely cultured with FBS. Platelet Lysate (PL), a hemoderivate enriched with growth factors and cytokines has been largely tested in several clinical applications and as substitute of FBS in culture. Here, we investigate whether PL can exert biological effects on TF populations similarly to other cell types. Results show that PL significantly enhances cell proliferation and migration vs. FBS, without influencing cell size/granularity. Upregulation of EGF, VEGF, KDR, MMP2-9, FAK mRNA levels also occurs and phosphorylation of AKT but not of ERK1/2 is significantly enhanced. The inhibition of the PI3kinase/AKT pathway with the specific inhibitor wortmannin, decreases PL-induced cell migration but not proliferation. Condition supernatants containing PL show increased bioavailability of Nitric Oxide and reduced levels of 8-Iso-PGF2-alpha, correlating with cell proliferation and migration. Pro-angiogenic/inflammatory soluble factors (GRO, Angiogenin, EGF, I-309, PARC) are exclusively or greater expressed in media containing PL than FBS. GMP-grade PL preparations positively influence in vitro biological effects of TFs representing a suitable and safer alternative to FBS.
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Augusto
authors.initials: A
authors.lastname: Carducci
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Gaia
authors.initials: G
authors.lastname: Scafetta
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Siciliano
authors.affiliation: Department of Internal Medicine and Medical Specialties, University of Rome, Sapienza, Rome, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Carnevale
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Rosa
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Coccia
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mangino
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Bordin
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Enzo Maria
authors.initials: EM
authors.lastname: Vingolo
authors.affiliation: 5Immunohematology and Transfusion Medicine, San Camillo Forlanini Hospital, Rome, Italy and Department of Experimental Medicine, University of Rome, Sapienza, Rome, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Pierelli
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Lendaro
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ragona
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Frati
authors.affiliation: Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, C.so della Repubblica 79, 04100 Latina, Italy, elena.defalco@uniroma1.it.
authors.forename: Elena
authors.initials: E
authors.lastname: De Falco
mesh_terms: D001792:Blood Platelets; D002465:Cell Movement; D049109:Cell Proliferation; D048429:Cell Size; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D058475:Tenon Capsule / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned