title: Understanding the Robust Physisorption between Bovine Serum Albumin and Amphiphilic Polymer Coated Nanoparticles.
abstract: The robust physisorption between nanoparticles (NPs) and proteins has attracted increasing attention due to the significance for both conjugation techniques and protein's corona formation at the bionano interface. In the present study, we first explored the possible binding sites of the bovine serum albumin (BSA) on amphiphilic polymer coated gold nanoparticles (AP-AuNPs). By using mass spectrometry, a 105-amino-acid peptide (12.2 kDa) is discovered as the possible "epitope" responsible for the robust physisorption between BSA and AP-AuNPs. Second, with the help of nanometal surface energy transfer (NSET) theory, we further found that the epitope peptide could insert at least 2.9 nm into the organic molecular layers of AP-AuNPs when the robust conjugates formed, which indicates how such a long epitope peptide can be accommodated by AP-AuNPs and resist protease's digestion. These findings might shed light on a new strategy for studying interactions between proteins and NPs, and further guide the rational design of NPs for safe and effective biomedical applications.
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Ruibo
authors.initials: R
authors.lastname: Zhong
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Yun
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Gong
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wei
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Agricultural Nanocenter, School of Life Science, Inner Mongolia Agricultural University , 306 Zhaowuda Road, Hohhot 010018, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D065966:Absorption, Physicochemical; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D000939:Epitopes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007052:Ibuprofen / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000939:Epitopes; D010455:Peptides; D011108:Polymers; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D006046:Gold; D007052:Ibuprofen
keywords: BSA; amphiphilic polymer; binding site; gold nanoparticle; nanometal surface energy transfer