title: Enhanced succinic acid production from corncob hydrolysate by microbial electrolysis cells.
abstract: In this study, Actinobacillus succinogenes NJ113 microbial electrolysis cells (MECs) were used to enhance the reducing power responsible for succinic acid production from corncob hydrolysate. During corncob hydrolysate fermentation, electric MECs resulted in a 1.31-fold increase in succinic acid production and a 1.33-fold increase in the reducing power compared with those in non-electric MECs. When the hydrolysate was detoxified by combining Ca(OH)2, NaOH, and activated carbon, succinic acid production increased from 3.47 to 6.95 g/l. Using a constant potential of -1.8 V further increased succinic acid production to 7.18 g/l. A total of 18.09 g/l of succinic acid and a yield of 0.60 g/g total sugar were obtained after a 60-h fermentation when NaOH was used as a pH regulator. The improved succinic acid yield from corncob hydrolysate fermentation using A. succinogenes NJ113 in electric MECs demonstrates the great potential of using biomass as a feedstock to cost-effectively produce succinate.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China. Electronic address: kqchen@njtech.edu.cn.
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D000188:Actinobacillus / Q000187:drug effects / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D018533:Biomass; D002244:Carbon / Q000494:pharmacology*; D004560:Electricity; D004572:Electrolysis / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D019802:Succinic Acid / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002244:Carbon; D019802:Succinic Acid
keywords: Actinobacillus succinogenes; Corncob hydrolysate; Microbial electrolysis cells; Succinic acid