title: Green electrospun grape seed extract-loaded silk fibroin nanofibrous mats with excellent cytocompatibility and antioxidant effect.
abstract: Silk fibroin (SF) from Bombyx mori has an excellent biocompatibility and thus be widely applied in the biomedical field. Recently, various SF-based composite nanofibers have been developed for more demanding applications. Additionally, grape seed extract (GSE) has been demonstrated to be powerful on antioxidation. In the present study, we dedicate to fabricate a GSE-loaded SF/polyethylene oxide (PEO) composite nanofiber by green electrospinning. Our results indicated the successful loading of GSE into the SF/PEO composite nanofibers. The introduction of GSE did not affect the morphology of the SF/PEO nanofibers and GSE can be released from the nanofibers with a sustained manner. Furthermore, comparing with the raw SF/PEO nanofibrous mats, the GSE-loaded SF/PEO nanofibrous mats significantly enhanced the proliferation of the skin fibroblasts and also protected them against the damage from tert-butyl hydroperoxide-induced oxidative stress. All these findings suggest a promising potential of this novel GSE-loaded SF/PEO composite nanofibrous mats applied in skin care, tissue regeneration and wound healing.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Lin
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Chen
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Huayue
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Linpeng
authors.initials: L
authors.lastname: Fan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China; Key Laboratory of Textile Science and Technology, Ministry of Education, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Xiangxin
authors.initials: X
authors.lastname: Lou
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China; Key Laboratory of Textile Science and Technology, Ministry of Education, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Chuanglong
authors.initials: C
authors.lastname: He
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China; Key Laboratory of Textile Science and Technology, Ministry of Education, Donghua University, Shanghai 201620, P.R. China. Electronic address: whs@dhu.edu.cn.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D012831:Bombyx; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D065546:Drug Liberation; D055664:Electrochemical Techniques; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry*; D056604:Grape Seed Extract / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D055772:Green Chemistry Technology; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D016877:Oxidants / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry; D012867:Skin / Q000166:cytology / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D056604:Grape Seed Extract; D016877:Oxidants; D011092:Polyethylene Glycols; D005349:Fibroins; D020122:tert-Butylhydroperoxide
keywords: Antioxidation; Grape seed extract; Green electrospinning; Silk fibroin