title: Cyclic dinucleotide detection with riboswitch-G-quadruplex hybrid.
abstract: A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split G-quadruplex for this conditional peroxidase-mimicking riboswitch.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Genichiro
authors.initials: G
authors.lastname: Tsuji
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA and Center for Drug Discovery, Purdue University, West Lafayette, IN 47907, USA. hsintim@purdue.edu.
authors.forename: Herman O
authors.initials: HO
authors.lastname: Sintim
mesh_terms: D001483:Base Sequence; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000032:analysis; D019281:Dimerization; D005453:Fluorescence; D054856:G-Quadruplexes; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D058928:Riboswitch; D014439:Tyramine
chemical_list: D009692:Nucleic Acid Heteroduplexes; D058928:Riboswitch; C062025:bis(3',5')-cyclic diguanylic acid; D009195:Peroxidase; D006152:Cyclic GMP; D014439:Tyramine