title: Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry.
abstract: Understanding how biological molecules are generated, metabolized and eliminated in living systems is important for interpreting processes such as immune response and disease pathology. While genomic and proteomic studies have provided vast amounts of information over the last several decades, interest in lipidomics has also grown due to improved analytical technologies revealing altered lipid metabolism in type 2 diabetes, cancer, and lipid storage disease. Mass spectrometry (MS) measurements are currently the dominant approach for characterizing the lipidome by providing detailed information on the spatial and temporal composition of lipids. However, interpreting lipids' biological roles is challenging due to the existence of numerous structural and stereoisomers (i.e. distinct acyl chain and double-bond positions), which are often unresolvable using present approaches. Here we show that combining liquid chromatography (LC) and structurally-based ion mobility spectrometry (IMS) measurement with MS analyses distinguishes lipid isomers and allows insight into biological and disease processes.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Kyle
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Karl K
authors.initials: KK
authors.lastname: Weitz
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Monroe
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Yehia M
authors.initials: YM
authors.lastname: Ibrahim
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.affiliation: Cincinnati Children's Hospital, Cincinnati, OH, USA.
authors.forename: Jeeyeon
authors.initials: J
authors.lastname: Cha
authors.affiliation: Cincinnati Children's Hospital, Cincinnati, OH, USA.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Erica S
authors.initials: ES
authors.lastname: Lovelace
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Wagoner
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, WA, USA and Department of Global Health, University of Washington, Seattle, WA, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Polyak
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
authors.affiliation: Cincinnati Children's Hospital, Cincinnati, OH, USA.
authors.forename: Sudhansu K
authors.initials: SK
authors.lastname: Dey
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Burnum-Johnson
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov kristin.burnum-johnson@pnnl.gov.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D005227:Fatty Acids; D008055:Lipids