title: Microscopy Image Browser: A Platform for Segmentation and Analysis of Multidimensional Datasets.
abstract: Understanding the structure-function relationship of cells and organelles in their natural context requires multidimensional imaging. As techniques for multimodal 3-D imaging have become more accessible, effective processing, visualization, and analysis of large datasets are posing a bottleneck for the workflow. Here, we present a new software package for high-performance segmentation and image processing of multidimensional datasets that improves and facilitates the full utilization and quantitative analysis of acquired data, which is freely available from a dedicated website. The open-source environment enables modification and insertion of new plug-ins to customize the program for specific needs. We provide practical examples of program features used for processing, segmentation and analysis of light and electron microscopy datasets, and detailed tutorials to enable users to rapidly and thoroughly learn how to use the program.
authors.affiliation: Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Ilya
authors.initials: I
authors.lastname: Belevich
authors.affiliation: Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Joensuu
authors.affiliation: Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Darshan
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Vihinen
authors.affiliation: Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Eija
authors.initials: E
authors.lastname: Jokitalo
mesh_terms: D007091:Image Processing, Computer-Assisted; D008853:Microscopy; D012984:Software