title: IVT-SAPAS: Low-Input and Rapid Method for Sequencing Alternative Polyadenylation Sites.
abstract: Gene transcribing with alternative polyadenylation (APA) sites leads to mRNA isoforms, which may encode different proteins or harbor different 3'UTRs. APA plays an important role in regulating gene expression network among various physiological processes, such as development, immune responses and cancer. Several methods of library construction for APA study have been developed to apply high-throughput sequencing. However, the requirement of high-input RNA and time-consuming nature of the current methods limited the studies of APA for the samples difficult to obtain. Here, we describe a new method based on our SAPAS in combining in vitro transcription (IVT) and magnetic beads purification. The new IVT-SAPAS provides a rapid and high-parallel procedure for APA library construction with low-input sample, which may be a new robust approach for studying APA.
authors.affiliation: Shenzhen Research Center of State Key Laboratory for Biocontrol, Research Institute of Sun Yat-sen University at Shenzhen, Shenzhen Virtual University Park, Hi-tech Industrial Park, Nanshan District, Shenzhen, 518057, P. R. China.|State Key Laboratory for Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, P. R. China.
authors.forename: Yonggui
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, P. R. China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Ge
authors.affiliation: State Key Laboratory for Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, P. R. China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wan
authors.affiliation: The Sixth Affiliated Hospital, Sun Yat-Sen University, 26 Yuancun Erheng Rd, Guangzhou, Guangdong, 510655, P. R. China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, 510006, P. R. China.|Beijing University of Chinese Medicine, 11 Bei San Huan Dong Road, Chao-yang District, Beijing, 100029, P. R. China.
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001665:Binding Sites; D006801:Humans; D061986:MCF-7 Cells; D059346:Magnets; D008863:Microspheres; D026723:Polyadenylation; D017423:Sequence Analysis, RNA / Q000379:methods*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions