title: Comparison of submerged and solid state pretreatment of sugarcane bagasse by Pandoraea sp. ISTKB: Enzymatic and structural analysis.
abstract: Pretreatment of sugarcane bagasse by Pandoraea sp. ISTKB was evaluated under submerged (SmF) and solid state (SSF) culture conditions. Number of bacteria was 2.7 times higher in SmF as compared with SSF. Enzymes produced under SmF were xylanase, LiP, MnP and laccase. In SSF xylanase and laccase were detected. CMCase, FPase and β-glucosidase were not detected. Delignification was highest in SmF with 19.94% and 10.43% removal of hemicelluloses and lignin, respectively. FTIR analysis suggested the degradation of lignin/hemicellulose component. SEM analysis showed pores were three times bigger in SmF as compared with raw bagasse. Maximum CR dye was absorbed by treated SmF bagasse. Enzymatic saccharification increased by 3.7 times after SmF treatment in comparison to raw bagasse. Pretreatment of bagasse by Pandoraea sp. ISTKB was more efficient under SmF than SSF. High negative correlation between saccharification vs lignin/hemicelluloses content justified the need for pretreatment of lignocellulosic waste before saccharification.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Madan
authors.initials: M
authors.lastname: Kumar
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Singhal
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: indushekhart@gmail.com.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D042521:Burkholderiaceae / Q000378:metabolism*; D002480:Cellulase / Q000032:analysis; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D042845:Laccase / Q000032:analysis; D008031:Lignin / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D031786:Saccharum; D001617:beta-Glucosidase / Q000032:analysis
chemical_list: D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D042845:Laccase; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase
keywords: Pandoraea; Pretreatment; Soild state fermentation; Submerged fermentation; Sugarcane bagasse