title: Non-isotopic Method for In Situ LncRNA Visualization and Quantitation.
abstract: In mammals and other eukaryotes, most of the genome is transcribed in a developmentally regulated manner to produce large numbers of long noncoding RNAs (lncRNAs). Genome-wide studies have identified thousands of lncRNAs lacking protein-coding capacity. RNA in situ hybridization technique is especially beneficial for the visualization of RNA (mRNA and lncRNA) expression in a heterogeneous population of cells/tissues; however its utility has been hampered by complicated procedures typically developed and optimized for the detection of a specific gene and therefore not amenable to a wide variety of genes and tissues.Recently, bDNA has revolutionized RNA in situ detection with fully optimized, robust assays for the detection of any mRNA and lncRNA targets in formalin-fixed paraffin-embedded (FFPE) and fresh frozen tissue sections using manual processing.
authors.affiliation: Affymetrix, 3420 Central Expressway, Santa Clara, CA, 95051, USA.
authors.forename: Botoul
authors.initials: B
authors.lastname: Maqsodi
authors.affiliation: Affymetrix, 3420 Central Expressway, Santa Clara, CA, 95051, USA. Corina_Nikoloff@affymetrix.com.
authors.forename: Corina
authors.initials: C
authors.lastname: Nikoloff
mesh_terms: D000818:Animals; D021121:Branched DNA Signal Amplification Assay / Q000379:methods*; D015925:Cryopreservation; D005557:Formaldehyde / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods; D000882:Haplorhini; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D016612:Paraffin Embedding / Q000379:methods; D062085:RNA, Long Noncoding / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D012474:Salmon; D016707:Tissue Fixation / Q000379:methods
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D005557:Formaldehyde
keywords: Branched DNA assay; bDNA assay; lncRNA in situ hybridization; lncRNA visualization