title: Anti-hyaluronidase Activity in Vitro and Amelioration of Mouse Experimental Dermatitis by Tomato Saponin, Esculeoside A.
abstract: The increasing incidence of atopic dermatitis during recent decades has prompted the development of safe and effective agents for prevention of atopic diseases. Esculeoside A, a glycoside of spirosolane type, is identified as a major component in ripe tomato fruits. The present study investigated the effects of esculeoside A and its aglycon esculeogenin A on hyaluronidase activity in vitro and antiallergy in experimental dermatitis mice. Esculeogenin A/esculeoside A (esculeogenin A equivalent) with an IC50 of about 2 μM/9 μM dose-dependently inhibited hyaluronidase activity measured by a modified Morgan-Elson method. Oral treatment with esculeoside A 10 mg/kg of experimental dermatitis mice for 4 weeks significantly decreased the skin clinical score to 2.5 without any detectable side effects compared with 6.75 of the control. The scratching frequency of esculeoside A 100 mg/kg application was decreased significantly as 107.5 times compared with 296.67 times of the control. Thus, the present study showed that esculeoside A/esculeogenin A significantly blocks hyaluronidase activity in vitro and that esculeoside A ameliorates mouse experimental dermatitis.
authors.affiliation: Department of Presymptomatic Medical Pharmacology, Faculty of Pharmaceutical Sciences, Sojo University , Kumamoto 860-0082, Japan.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhou
authors.affiliation: Department of Presymptomatic Medical Pharmacology, Faculty of Pharmaceutical Sciences, Sojo University , Kumamoto 860-0082, Japan.
authors.forename: Yurina
authors.initials: Y
authors.lastname: Kanda
authors.affiliation: Department of Presymptomatic Medical Pharmacology, Faculty of Pharmaceutical Sciences, Sojo University , Kumamoto 860-0082, Japan.
authors.forename: Anna
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Department of Presymptomatic Medical Pharmacology, Faculty of Pharmaceutical Sciences, Sojo University , Kumamoto 860-0082, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Manabe
authors.affiliation: Department of Presymptomatic Medical Pharmacology, Faculty of Pharmaceutical Sciences, Sojo University , Kumamoto 860-0082, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nohara
authors.affiliation: Department of Presymptomatic Medical Pharmacology, Faculty of Pharmaceutical Sciences, Sojo University , Kumamoto 860-0082, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Yokomizo
mesh_terms: D000818:Animals; D003872:Dermatitis / Q000188:drug therapy* / Q000201:enzymology; D004195:Disease Models, Animal; D005260:Female; D005638:Fruit / Q000737:chemistry; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000008:administration & dosage*; D012502:Sapogenins / Q000008:administration & dosage*
chemical_list: D010936:Plant Extracts; D012502:Sapogenins; C530544:esculeoside A; D006821:Hyaluronoglucosaminidase
keywords: anti-hyaluronidase; esculeogenin A; esculeoside A; experimental atopic dermatitis; tomato