title: MicroRNA-92a Regulates Expression of Kruppel-like Factor2 in Rabbit Model of Intracranial Aneurysm.
abstract: To explore role of miRNA-92a in regulation of KLF2 expression in intracranial aneurysm model, real time PCR, IHC, ISH and luciferase activity were used to test relationship of miR-92a and KLF2. The results demonstrated that KLF2 showed a time-dependent increase following development of IA model, but the miR-92a showed a time-dependent decrease, co-expression of miR-92a was found to effectively down-regulate luciferase expression in constructs with 3'UTRs of KLF2, to determine the functions of miR-92a in rabbit ECs, we transfected the pre-miR-92a into rabbit ECs for over-expression, the results showed that the protein level of KLF2 was significantly down-regulated when over-expression of miR-92a. In conclusion, our research demonstrated that miR-92a and KLF2 were negative correlation in intracranial aneurysm model, and miR-92a could directly target KLF2 in endothelial cells through complementary sequence of 3'UTR region.
authors.affiliation: Changhai Hospital Department of Neurosurgery Shanghai China.
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.affiliation: PLA 455 Hospital Department of Neurosurgery Shanghai China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Changhai Hospital Department of Neurosurgery Shanghai China.
authors.forename: Q
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Changhai Hospital Department of Neurosurgery Shanghai China.
authors.forename: P
authors.initials: P
authors.lastname: Yang
authors.affiliation: Changhai Hospital Department of Neurosurgery Shanghai China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: Changhai Hospital Department of Neurosurgery Shanghai China ljmjianmingliu@126.com.
authors.forename: J
authors.initials: J
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D002532:Intracranial Aneurysm / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D020413:3' Untranslated Regions; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; D008156:Luciferases