title: No evidence for oxidative stress in the cerebellar tissues or cells of juvenile male mice exposed via lactation to the 6 non-dioxin-like PCBs at levels below the regulatory safe limits for humans.
abstract: The developing central nervous system is particularly vulnerable to environmental contaminants such as non-dioxin-like polychlorinated biphenyls (NDL-PCBs). This study investigated the potential oxidative effects in mice pups exposed via lactation to the sum of the six indicator NDL-PCBs (∑6 NDL-PCBs) at 0, 1, 10 and 100 ng/kg per 14 days, constituting levels below the guidance values fixed by French food safety agencies for humans at 10 ng/kg body weight per day. For this purpose, the oxidative status was assessed by flow cytometry via dichloro-dihydro-fluorescein diacetate in the cerebellum of juvenile male offspring mice during brain growth spurt [postnatal day (PND) 14]. No significant differences were found in the levels of reactive oxygen species in the cerebellar neurons or glial cells (astrocytes, oligodendrocytes and microglia) of lactationally exposed male mice at PND 14 (p>0.05). Concordantly, oxidative-stress related gene expression was measured by qPCR for catalase, copper zinc superoxide dismutase 1, glyoxalase 1, glutathione peroxidase 1, and glutathione reductase 1, in the cerebellum at PND 14 appeared unaffected, as also verified at the protein level by immunoblots. Moreover, transcriptomic data from our previous work have not shown differences in the mRNA expressions of genes belonging to GO terms involved in oxidative stress in neurons of male mice exposed to ∑6 NDL-PCBs compared to controls; except for glyoxalase 1 which was downregulated in neurons isolated from exposed group compared to controls. Our findings suggest that lactational exposure to NDL-PCBs at environmental relevant concentrations may not cause significant oxidative effect on juvenile cerebellum.
authors.affiliation: Neurotoxicologie Alimentaire et Bioactivité, MRCA, BP 4102, 57040 Metz, France; Université de Lorraine, Unité de Recherche Animal et Fonctionnalités des Produits Animaux (UR AFPA), EA3998, INRA USC 0340, France. Electronic address: arpine.elnar@hotmail.fr.
authors.forename: Arpiné Ardzivian
authors.initials: AA
authors.lastname: Elnar
authors.affiliation: Neurotoxicologie Alimentaire et Bioactivité, MRCA, BP 4102, 57040 Metz, France; Université de Lorraine, Unité de Recherche Animal et Fonctionnalités des Produits Animaux (UR AFPA), EA3998, INRA USC 0340, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Desor
authors.affiliation: Luxembourgish Institute of Science and Technology, Environmental Research and Innovation Department, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Legay
authors.affiliation: Université de Lorraine, UMR INSERM-954 Nutrition - Génétique et exposition aux risques environnementaux, Faculté de médecine de Nancy, 54511 Vandœuvre-Lès-Nancy, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Nemos
authors.affiliation: Biodisponibilité et Fonctionnalités des Lipides Alimentaires, BFLA, ENSAIA, Avenue de la forêt de Haye, 54500 Vandoeuvre-lès-Nancy, France; Université de Lorraine, Unité de Recherche Animal et Fonctionnalités des Produits Animaux (UR AFPA), EA3998, INRA USC 0340, France.
authors.forename: Frances T
authors.initials: FT
authors.lastname: Yen
authors.affiliation: Biodisponibilité et Fonctionnalités des Lipides Alimentaires, BFLA, ENSAIA, Avenue de la forêt de Haye, 54500 Vandoeuvre-lès-Nancy, France; Université de Lorraine, Unité de Recherche Animal et Fonctionnalités des Produits Animaux (UR AFPA), EA3998, INRA USC 0340, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Oster
authors.affiliation: Luxembourgish Institute of Science and Technology, Environmental Research and Innovation Department, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
authors.forename: Torsten
authors.initials: T
authors.lastname: Bohn
authors.affiliation: Neurotoxicologie Alimentaire et Bioactivité, MRCA, BP 4102, 57040 Metz, France; Université de Lorraine, Unité de Recherche Animal et Fonctionnalités des Produits Animaux (UR AFPA), EA3998, INRA USC 0340, France.
authors.forename: Rachid
authors.initials: R
authors.lastname: Soulimani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000254:growth & development; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D005260:Female; D059022:Food Safety; D007774:Lactation; D008297:Male; D051379:Mice; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D011078:Polychlorinated Biphenyls
keywords: Central nervous system; Development; Glial cells; Lactational exposure; Neurons; Non-dioxin-like polychlorinated biphenyls; Oxidative stress