title: In Situ Monitoring of the Intracellular Stability of Nanoparticles by Using Fluorescence Lifetime Imaging.
abstract: FLIMaging nanoparticle degradation: semiconductor and metal nanoparticle degradation has been observed in live cells over 3 d via the change of the characteristic luminescence lifetime using fluorescence lifetime imaging microscopy (FLIM). Thus, FLIM is a simple yet robust tool to examine the intracellular stability of photoluminescent nanoparticles in live cells, tissues, and organisms.
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.
authors.forename: Li
authors.initials: L
authors.lastname: Shang
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.
authors.forename: Linxiao
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.
authors.forename: Haixia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.|Institute of Nanotechnology and Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany.|Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Gerd Ulrich
authors.initials: GU
authors.lastname: Nienhaus
mesh_terms: D006046:Gold; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry*; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D013997:Time Factors
chemical_list: D006046:Gold
keywords: fluorescence lifetime imaging microscopy; live cells; luminescent probes; nanoparticles; quantum dots