title: The Role of Ozone Therapy in Hepatic Fibrosis due to Biliary Tract Obstruction.
abstract: OBJECTIVE
  In this experimental study, we investigated the role of ozone therapy in hepatic fibrosis caused by biliary obstruction.
  
  
  METHODS
  In this study, 21 male Sprague-Dawley rats were divided equally into three groups. In the control group, only laparotomy was performed and intraperitoneal cavity is washed with 1 mL of saline. In the sham group (SG), common bile duct is binded with laparotomy and no treatment is given afterward. In the experimental group (EG) after the binding of common bile duct with laparotomy, 1 mg/kg dose and 50 mg/mL concentration of ozone were applied rectally for 21 days. Hepatic tissue sample and intracardiac blood samples were collected from all animals at postoperative 21st day with relaparatomy.
  
  
  RESULTS
  When we compared the experiment to SG, we detected a decrease in aspartate aminotransferase, alkaline phosphate (ALP), total bilirubin, and direct bilirubin levels in the EG, however, only the decrease in total bilirubin levels were statistically significant (p = 0.025). Histopathological examination of livers of rats in the EG showed lower level of hepatic fibrosis and inflammation. In the SG, incomplete cirrhosis was detected in 57.1% of the rats, whereas in the EG, no cirrhosis was detected. Immunohistochemically, periportal inflammation was 100% in the SG, whereas it was seen (3/7) 42.9% in the EG. A significant decrease in positive α-smooth muscle actin reaction was observed in ozone-treated group compared with SG.
  
  
  CONCLUSIONS
  We suggest that ozone can decrease the hepatic destruction levels in experimental model of biliary obstruction.
authors.affiliation: Department of Paediatric Surgery, Istanbul University, Istanbul, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Kocaman
authors.affiliation: Department of Paediatric Surgery, Istanbul University, Istanbul, Turkey.
authors.forename: Basak
authors.initials: B
authors.lastname: Erginel
authors.affiliation: Department of Pathology, Istanbul University, Istanbul, Turkey.
authors.forename: Semen Yesil
authors.initials: SY
authors.lastname: Onder
authors.affiliation: Department of Paediatric Surgery, Istanbul University, Istanbul, Turkey.
authors.forename: Feryal Gun
authors.initials: FG
authors.lastname: Soysal
authors.affiliation: Department of Paediatric Surgery, Istanbul University, Istanbul, Turkey.
authors.forename: Erbug
authors.initials: E
authors.lastname: Keskin
authors.affiliation: Department of Paediatric Surgery, Istanbul University, Istanbul, Turkey.
authors.forename: Alaaddin
authors.initials: A
authors.lastname: Celik
authors.affiliation: Department of Paediatric Surgery, Istanbul University, Istanbul, Turkey.
authors.forename: Tansu
authors.initials: T
authors.lastname: Salman
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D002779:Cholestasis / Q000150:complications*; D004334:Drug Administration Schedule; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D010083:Oxidants, Photochemical / Q000627:therapeutic use*; D010126:Ozone / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D010083:Oxidants, Photochemical; D010126:Ozone