title: MicroRNA-145 inhibits growth and migration of breast cancer cells through targeting oncoprotein ROCK1.
abstract: MicroRNAs are small non-coding RNAs that may also function as oncogenes and tumor suppressor genes, as the abnormal expression of microRNAs is associated with various human tumors. MicroRNA-145 (miR-145) inhibits growth and increases chemo- or radiosensitivity in various cancers. However, the role of miR-145 in breast cancer progression remains unknown. In this study, miR-145 expression level was measured via quantitative real-time PCR in 88 pairs of human breast cancer tissues and adjacent normal tissues and in a panel of human breast cancer cell lines. Cell proliferation and cell migration were assessed by cell viability assay and transwell assay. Western blot was used to verify Rho-associated protein kinase 1 (ROCK1) as a novel target gene of miR-145. Our results showed that miR-145 was frequently downregulated in breast cancer tissues and cell lines. Overexpression of miR-145 in MCF-7 and BT-549 cell lines significantly inhibited cell proliferation, migration, and invasion in vitro. ROCK1 was identified as a target of miR-145, and ectopic expression of miR-145 downregulated ROCK1. Our findings indicate that miR-145 acts as a tumor suppressor and its downregulation in tumor tissues may contribute to the progression of breast cancer through a mechanism involving ROCK1, suggesting miR-145 as a potential new diagnostic and therapeutic target for the treatment of breast cancer.
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital and Institute, 440 Jiyan Road, Jinan, 250117, Shandong, China.
authors.forename: Meizhu
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Gastrointestinal Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, Jinan, 250117, Shandong, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital and Institute, 440 Jiyan Road, Jinan, 250117, Shandong, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital and Institute, 440 Jiyan Road, Jinan, 250117, Shandong, China. dyzuowenshu67@163.com.
authors.forename: Wenshu
authors.initials: W
authors.lastname: Zuo
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases
keywords: Breast cancer; MiR-145; Migration; Proliferation; ROCK1