title: MicroRNA-592 targets DEK oncogene and suppresses cell growth in the hepatocellular carcinoma cell line HepG2.
abstract: MicroRNAs (miRNAs) are classes of small, non-coding RNAs that regulate the translation of target mRNA transcripts. In this study, we demonstrated that miR-592 was downregulated in human hepatocellular carcinoma (HCC) and could suppress growth of the human HCC cell line HepG2. A tumor oncogene, DEK, was identified as a direct target of miR-592. Luciferase report assay indicated miR-592 regulates DEK expression though bind to its 3'UTR. Furthermore, knockdown of DEK also suppressed cell proliferation of HepG2 cells, which was consist with miR-592. At last, we suggested that DEK was upregulated in HCC tissues inversely with miR-592. These results demonstrated that miR-592 targets DEK transcript and suppresses HCC cell growth, and may provide potential therapeutic target in human HCC.
authors.affiliation: Department of Microbiology and Immunology, Changchun University of Chinese Medicine Changchun 130177, Jilin Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Microbiology and Immunology, Changchun University of Chinese Medicine Changchun 130177, Jilin Province, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Immunology, Changchun University of Chinese Medicine Changchun 130177, Jilin Province, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Microbiology and Immunology, Changchun University of Chinese Medicine Changchun 130177, Jilin Province, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Yue
authors.affiliation: Department of Microbiology and Immunology, Changchun University of Chinese Medicine Changchun 130177, Jilin Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Su
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics*; D000075223:Poly-ADP-Ribose Binding Proteins; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; C000588991:MIRN592 microRNA, human; D035683:MicroRNAs; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins
keywords: DEK; cell growth; hepatocellular carcinoma; miR-592