title: Photovoltaic surfaces enable clonal myoblastic cell release using visible light as external stimulation.
abstract: Many new biomedical approaches to treating disease require the supply of cells delivered to an injured or diseased organ either individually, collectively as aggregates or sheets, or encapsulated with a scaffold. The collection of cells is accomplished by using enzymatic digestion witch suffer from the need to remove the enzymes after digestion. In addition, enzymatic methods are not applicable for all cells, cell aggregates, cell sheets or 3D structures. The objective of this study was to investigate the release of cultured cells from silicon based Photovoltaic (PV) surfaces using a light source as external stimulation. C2C12 myoblasts were cultured on the negative surface of a PV device and upon confluence they were exposed to light. The amount of released cells was quantified as a function light exposure. It was found that light exposure at 25,000 lux for one hour caused equivalent cell release from the PV surface than trypsination. The released cells are viable and can be re-cultured if needed. This mechanism may offer an alternative method to release excitable cells without using an enzymatic agent. This may be important for cell therapy if larger cell structures such as sheets need to be collected.
authors.affiliation: Biomedical Engineering, The University of Texas at El Paso, El Paso, TX, USA.
authors.forename: Mohammod Kabir
authors.initials: MK
authors.lastname: Bhuyan
authors.affiliation: Bioengineering, Clemson University, Clemson, SC, USA.
authors.forename: Jorge
authors.initials: J
authors.lastname: Rodriguez-Devora
authors.affiliation: Industrial, Manufacturing & Systems Engineering, The University of Texas at El Paso, El Paso, TX, USA.
authors.forename: Tzu-Liang Bill
authors.initials: TL
authors.lastname: Tseng
authors.affiliation: Biomedical Engineering, The University of Texas at El Paso, El Paso, TX, USA. tboland@utep.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Boland
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D002470:Cell Survival; D008027:Light; D032446:Myoblasts / Q000166:cytology*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D012825:Silicon
keywords: Cell release; Excitable cells; Light stimulated cell release; Trypsin alternative