title: Screening of intracerebral hemorrhage associated allele combinations at different loci using a novel association analysis.
abstract: BACKGROUND
  Genetic research has progressed along with scientific and technological developments. However, it is difficult to identify frequency differences in the allele combination at cross-loci.
  
  
  OBJECTIVE
  The purpose of this study was to examine the relationship between the presence of specific allele combinations of short tandem repeat (STR) loci and the onset of intracerebral hemorrhage (ICH) using a novel methodology.
  
  
  METHODS
  DNA samples were collected from patients with ICH, who were adult population. There were a total of 51 Chinese patients (102 chromosomes), comprising 30 males and 21 females. Alleles from short tandem repeat (STR) loci were determined using the STR Profiler Plus PCR amplification kit (15 STR loci). Statistically significant differences between observed and expected frequencies of allele combinations were identified. To further determine allele combinations related to the disease, analyses of patient age at disease onset for those carrying a specific allele combination were conducted. Finally, cross-validation of the two sets of analytical results was carried out.
  
  
  RESULTS
  A total of 1550 pairwise combinations were obtained by computer counting, of which eight pairs of alleles showed significant differences between the observed and expected frequencies (p<0.05, from 0.006 to 0.042). The p value for the cross-validation analysis was less than 0.05 for two pairs of alleles (D13S317-11 and vWA-17, p=0.021; D7S820-13 and D2S1338-18, p=0.023).
  
  
  CONCLUSIONS
  The study identified each population had a unique gene distribution and that distribution followed certain rules. ICH onset may be associated with this allele combinations (D13S317-11 and vWA-17; D7S820-13 and D2S1338-18). The new methodology used in this study could enable additional discoveries pertaining to the relationship between specific allele combinations at different loci and the onset of complex diseases.
authors.affiliation: College of Medical Laboratory, Dalian Medical University, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Gai
authors.affiliation: College of Medical Laboratory, Dalian Medical University, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Medical Laboratory, Dalian Medical University, China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Yu
authors.affiliation: College of Medical Laboratory, Dalian Medical University, China. Electronic address: liuhui60@sina.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
mesh_terms: D017668:Age of Onset; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002543:Cerebral Hemorrhage / Q000235:genetics*; D002681:China; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged
keywords: Genetic analysis; Heredity; Intracerebral haemorrhage (ICH)