title: [Diagnosis of Muscular Dystrophy Using Western Blot with Micro-sample of Muscle].
abstract: OBJECTIVE
  To diagnose muscular dystrophy using Western blot (WB) by improving the method of the protein extraction.
  
  
  METHODS
  Firstly,we compared the effect of different sample buffer solutions and processing Methods on the extraction of muscle protein in rats,then selected the appropriate extracting method and the process of the muscular protein.
  
  
  RESULTS
  We put the selected sample buffer into the micro-sample,then mixed. The concentration of the extracting protein was much more,and the loss during the process was much less. We extracted enough protein in 62 cases. The protein bands were showed clearly by WB,and the abnormal protein bands were shown in some patients. Compared with the Results of immunohistochemical staining detected the severe abnormal expressions of Dys-R,Dys-C,and Dys-N in the specimens,we did not detect the corresponding target band in WB. We detected the target protein band of the specimens were abnormal position,light or normal staining in WB,while Dys were mildly expressed in immunohistochemical staining.
  
  
  CONCLUSIONS
  The improved protein extraction method can save the muscle tissue,and the protein bands can be used for diagnosing the muscular dystrophy. For clinically suspected patients with dystrophinopathy,if normal or mild deficiency is shown by immunohistochemistry,WB should be applied to detect the dystrophin protein band.
authors.affiliation: Department of Neurology,China-Japan Friendship Hospital,Beijing 100029,China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Wu
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Yan-huan
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Ren
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Guan
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Wei
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Min
authors.initials: M
authors.lastname: Qian
authors.forename: Yu-pu
authors.initials: YP
authors.lastname: Guo
authors.affiliation: Department of Neurology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Cui
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Jiao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016189:Dystrophin; D006801:Humans; D007150:Immunohistochemistry; D009136:Muscular Dystrophies; D021381:Protein Transport; D051381:Rats; D013194:Staining and Labeling
chemical_list: D016189:Dystrophin