title: Binding kinetics of membrane-anchored receptors and ligands: Molecular dynamics simulations and theory.
abstract: The adhesion of biological membranes is mediated by the binding of membrane-anchored receptor and ligand proteins. Central questions are how the binding kinetics of these proteins is affected by the membranes and by the membrane anchoring of the proteins. In this article, we (i) present detailed data for the binding of membrane-anchored proteins from coarse-grained molecular dynamics simulations and (ii) provide a theory that describes how the binding kinetics depends on the average separation and thermal roughness of the adhering membranes and on the anchoring, lengths, and length variations of the proteins. An important element of our theory is the tilt of bound receptor-ligand complexes and transition-state complexes relative to the membrane normals. This tilt results from an interplay of the anchoring energy and rotational entropy of the complexes and facilitates the formation of receptor-ligand bonds at membrane separations smaller than the preferred separation for binding. In our simulations, we have considered both lipid-anchored and transmembrane receptor and ligand proteins. We find that the binding equilibrium constant and binding on-rate constant of lipid-anchored proteins are considerably smaller than the binding constant and on-rate constant of rigid transmembrane proteins with identical binding domains.
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, 14424 Postdam, Germany.
authors.forename: Jinglei
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, 14424 Postdam, Germany.
authors.forename: Guang-Kui
authors.initials: GK
authors.lastname: Xu
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, 14424 Postdam, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Lipowsky
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, 14424 Postdam, Germany.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Weikl
mesh_terms: D001665:Binding Sites; D007700:Kinetics; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry*; D056004:Molecular Dynamics Simulation
chemical_list: D008024:Ligands; D008565:Membrane Proteins