title: Hybrid microarray based on double biomolecular markers of DNA and carbohydrate for simultaneous genotypic and phenotypic detection of cholera toxin-producing Vibrio cholerae.
abstract: Life-threatening diarrheal cholera is usually caused by water or food contaminated with cholera toxin-producing Vibrio cholerae. For the prevention and surveillance of cholera, it is crucial to rapidly and precisely detect and identify the etiological causes, such as V. cholerae and/or its toxin. In the present work, we propose the use of a hybrid double biomolecular marker (DBM) microarray containing 16S rRNA-based DNA capture probe to genotypically identify V. cholerae and GM1 pentasaccharide capture probe to phenotypically detect cholera toxin. We employed a simple sample preparation method to directly obtain genomic DNA and secreted cholera toxin as target materials from bacterial cells. By utilizing the constructed DBM microarray and prepared samples, V. cholerae and cholera toxin were detected successfully, selectively, and simultaneously; the DBM microarray was able to analyze the pathogenicity of the identified V. cholerae regardless of whether the bacteria produces toxin. Therefore, our proposed DBM microarray is a new effective platform for identifying bacteria and analyzing bacterial pathogenicity simultaneously.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Hwa Hui
authors.initials: HH
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Seo
authors.affiliation: School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 712-749 Republic of Korea.
authors.forename: Chang Sup
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea.
authors.forename: Byeong Hee
authors.initials: BH
authors.lastname: Hwang
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. Electronic address: hjcha@postech.ac.kr.
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry / Q000235:genetics; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology; D002772:Cholera Toxin / Q000302:isolation & purification*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D046228:Microarray Analysis; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D014734:Vibrio cholerae / Q000302:isolation & purification*
chemical_list: D002241:Carbohydrates; D015342:DNA Probes; D012336:RNA, Ribosomal, 16S; D002772:Cholera Toxin
keywords: Carbohydrate probe; Cholera toxin; DNA probe; Double biomolecular markers; Pathogenicity; Vibrio cholerae