title: Dynamic loading, matrix maintenance and cell injection therapy of human intervertebral discs cultured in a bioreactor.
abstract: Low back pain originating from intervertebral disc (IVD) degeneration affects the quality of life for millions of people, and it is a major contributor to global healthcare costs. Long-term culture of intact IVDs is necessary to develop ex vivo models of human IVD degeneration and repair, where the relationship between mechanobiology, disc matrix composition and metabolism can be better understood. A bioreactor was developed that facilitates culture of intact human IVDs in a controlled, dynamically loaded environment. Tissue integrity and cell viability was evaluated under 3 different loading conditions: low 0.1-0.3, medium 0.1-0.3 and high 0.1-1.2 MPa. Cell viability was maintained > 80 % throughout the disc at low and medium loads, whereas it dropped to approximately 70 % (NP) and 50 % (AF) under high loads. Although cell viability was affected at high loads, there was no evidence of sGAG loss, changes in newly synthesised collagen type II or chondroadherin fragmentation. Sulphated GAG content remained at a stable level of approximately 50 µg sGAG/mg tissue in all loading protocols. To evaluate the feasibility of tissue repair strategies with cell supplementation, human NP cells were transplanted into discs within a thermoreversible hyaluronan hydrogel. The discs were loaded under medium loads, and the injected cells remained largely localised to the NP region. This study demonstrates the feasibility of culturing human IVDs for 14 days under cyclic dynamic loading conditions. The system allows the determination a safe range-of-loading and presents a platform to evaluate cell therapies and help to elucidate the effect of load following cell-based therapies.
authors.affiliation: McGill University Health Centre, Department of Surgery, Montreal General Hospital, Room C9.173, 1650 Cedar Ave, Montreal, QC H3G 1A4, Canada.lisbet.haglund@mcgill.ca.
authors.forename: D H
authors.initials: DH
authors.lastname: Rosenzweig
authors.forename: R
authors.initials: R
authors.lastname: Gawri
authors.forename: J
authors.initials: J
authors.lastname: Moir
authors.forename: L
authors.initials: L
authors.lastname: Beckman
authors.forename: D
authors.initials: D
authors.lastname: Eglin
authors.forename: T
authors.initials: T
authors.lastname: Steffen
authors.forename: P J
authors.initials: PJ
authors.lastname: Roughley
authors.forename: J A
authors.initials: JA
authors.lastname: Ouellet
authors.forename: L
authors.initials: L
authors.lastname: Haglund
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019149:Bioreactors; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002648:Child; D005260:Female; D048091:Guided Tissue Regeneration; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007403:Intervertebral Disc / Q000166:cytology*; D055959:Intervertebral Disc Degeneration / Q000473:pathology* / Q000628:therapy*; D017116:Low Back Pain / Q000209:etiology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009924:Organ Culture Techniques; D013312:Stress, Physiological / Q000502:physiology; D055815:Young Adult
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate