title: miR-98 suppresses tumor cell growth and metastasis by targeting IGF1R in oral squamous cell carcinoma.
abstract: Increasing evidences indicate that dysregulation of miRNAs contributes to the pathogenesis of oral squamous cell carcinoma (OSCC). However, little is known about the potential role of miR-98 in OSCC. Here, we found that miR-98 was downregulated in OSCC tissues and cell lines. Overexpression of miR-98 inhibited proliferation, colony formation, migration, and invasion of OSCC cells. IGF1R was identified as the potential target of miR-98 using dual luciferase assay, qRT-PCR and western blot. Furthermore, restoration of IGF1R remarkably reversed the tumor-suppressive effects of miR-98 on OSCC cells. Moreover, miR-98 expression was inversely correlated with IGF1R expression in 19 cases of OSCC. These findings suggest that miR-98 inhibits cancer cell growth and metastasis by direct targeting IGF1R, implicating miR-98 as a novel potential therapeutic target for OSCC.
authors.affiliation: Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University Wenzhou, 325000, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Gastroenterology, Jining NO.1 People's Hospital Jining, Shandong, 272011, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University Wenzhou, 325000, PR China.
authors.forename: Hanxiao
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University Wenzhou, 325000, PR China.
authors.forename: Songcheng
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Stomatology, Second Hospital of Tianjin Medical University Tianjin, 300211, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University Wenzhou, 325000, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D063646:Carcinogenesis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000235:genetics*
chemical_list: C000597236:IGF1R protein, human; C546084:MIRN98 microRNA, human; D035683:MicroRNAs; D017451:Receptors, Somatomedin; D017526:Receptor, IGF Type 1
keywords: IGF1R; Oral squamous cell carcinoma; invasion; miR-98; migration; proliferation