title: Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms.
abstract: Chronic acrylamide (ACR) exposure induces peripheral-central axonopathy in occupational workers and laboratory animals, but the underlying mechanisms remain unclear. In this study, we first investigated the effects of ACR on slow axonal transport of neurofilaments in cultured rat dorsal root ganglia (DRG) neurons through live-cell imaging approach. Then for the underlying mechanisms exploration, the protein level of neurofilament subunits, motor proteins kinesin and dynein, and dynamitin subunit of dynactin in DRG neurons were assessed by western blotting and the concentrations of ATP was detected using ATP Assay Kit. The results showed that ACR treatment results in a dose-dependent decrease of slow axonal transport of neurofilaments. Furthermore, ACR intoxication significantly increases the protein levels of the three neurofilament subunits (NF-L, NF-M, NF-H), kinesin, dynein, and dynamitin subunit of dynactin in DRG neurons. In addition, ATP level decreased significantly in ACR-treated DRG neurons. Our findings indicate that ACR exposure retards slow axonal transport of NF-M, and suggest that the increase of neurofilament cargoes, motor proteins, dynamitin of dynactin, and the inadequate ATP supply contribute to the ACR-induced retardation of slow axonal transport.
authors.affiliation: Institute of Toxicology, School of Public Health, Shandong University, Jinan, 250012, China.|Institute of Environment and Health, School of Public Health, Shandong University, Jinan, 250012, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: An
authors.affiliation: Beijing Municipal Institute of Labour Protection, Taoranting Road, Xicheng District, Beijing, 100054, China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Environment and Health, School of Public Health, Shandong University, Jinan, 250012, China.
authors.forename: Jiliang
authors.initials: J
authors.lastname: Si
authors.affiliation: Institute of Toxicology, School of Public Health, Shandong University, Jinan, 250012, China.
authors.forename: Cuili
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Han
authors.affiliation: Institute of Toxicology, School of Public Health, Shandong University, Jinan, 250012, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Toxicology, School of Public Health, Shandong University, Jinan, 250012, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Institute of Toxicology, School of Public Health, Shandong University, Jinan, 250012, China. keqinx@sdu.edu.cn.
authors.forename: Keqin
authors.initials: K
authors.lastname: Xie
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D002478:Cells, Cultured; D000072159:Dynactin Complex / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007382:Intermediate Filaments / Q000187:drug effects* / Q000502:physiology; D016547:Kinesins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D021122:Protein Subunits / Q000378:metabolism; D017207:Rats, Sprague-Dawley
chemical_list: D000072159:Dynactin Complex; D004785:Environmental Pollutants; D021122:Protein Subunits; D020106:Acrylamide; D000255:Adenosine Triphosphate; D004398:Dyneins; D016547:Kinesins
keywords: Acrylamide; Dynactin; Dynamitin; Motor proteins; Neurofilaments; Slow axonal transport