title: Antimicrobial activities, toxicity and phenolic composition of Asphodeline anatolica E. Tuzlaci leaf extracts from Turkey.
abstract: The antimicrobial activity of acetone, methanol and aqueous extracts of Asphodeline anatolica E. Tuzlaci leaves was evaluated against American type culture collection, food and clinical isolates (Listeria monocytogenes and Staphylococcus aureus including methicillin-resistant strains-MRSA). Biofilm formation, toxicity and characterisation of the polyphenolic content were analysed. The acetone extract demonstrated a higher antibacterial activity against S. aureus including MRSA strains, L. monocytogenes and Pseudomonas aeruginosa than against other extracts. No effect was observed in biofilm formation. The extracts resulted non-toxic against Artemia salina Leach. The phytochemical screening of extracts indicated that they mainly contained six polyphenols identified as catechin 3-O-gallate, protocatechuic acid, diosmin, rutin, cirsimaritin and kaempferol glucoside. This study is the first report on antimicrobial activity and phenolic content of A. anatolica and contributes to enrich the literature data on the biological properties of this plant. A. anatolica leaves have a potential as source of natural antimicrobial compounds.
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Andreana
authors.initials: A
authors.lastname: Marino
authors.affiliation: b Department of Biology , University of Selcuk , Konya , Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Zengin
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Nostro
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Ginestra
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.affiliation: c Department of the Environment, Security, Territory, Food, and Health Sciences , University of Messina , Messina , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Natalizia
authors.initials: N
authors.lastname: Miceli
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Taviano
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Filocamo
authors.affiliation: a Department of Pharmaceutical Sciences and Health Products , University of Messina , Messina , Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bisignano
authors.affiliation: b Department of Biology , University of Selcuk , Konya , Turkey.
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Aktumsek
keywords: Antimicrobials; Artemia salina test; HPLC analysis; Listeria monocytogenes; MRSA; biofilm