title: Cytocompatibility of amine functionalized carbon nanoparticles grafted on polyethylene.
abstract: Five types of amide-amine Carbon Nano-Particles (CNPs) were prepared by functionalization of CNPs and characterized by several analytical methods. The successful grafting of amines on CNPs was verified by X-ray photoelectron spectroscopy (XPS), organic elemental analysis and electrokinetic analysis. The size and morphology of CNPs were determined from transmission electron microscopy. The surface area and porosity of CNPs were examined by adsorption and desorption isotherms. Differential scanning calorimetry was used to investigate thermal stability of CNPs. The amount of bonded amine depends on its dimensionality arrangement. Surface area and pore volumes of CNPs decrease several times after individual amino-compound grafting. Selected types of functionalized CNPs were grafted onto a plasma activated surface of HDPE. The successful grafting of CNPs on the polymer surface was verified by XPS. Wettability was determined by contact angle measurements. Surface morphology and roughness were studied by atomic force microscopy. A dramatic decrease of contact angle and surface morphology was observed on CNP grafted polymer surface. Cytocompatibility of modified surfaces was studied in vitro, by determination of adhesion, proliferation and viability of vascular smooth muscle cells (VSMCs). Grafting of CNPs onto the polymer surface has a positive effect on the adhesion, proliferation and viability of VSMCs.
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, 166 28 Prague 6, Czech Republic. Electronic address: pavlina.zakova@vscht.cz.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Žáková
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, 166 28 Prague 6, Czech Republic.
authors.forename: Nikola
authors.initials: N
authors.lastname: Slepičková Kasálková
authors.affiliation: Faculty of Science, J. E. Purkyně University, Ústí nad Labem, Czech Republic.
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Kolská
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, 166 28 Prague 6, Czech Republic.
authors.forename: Jindřich
authors.initials: J
authors.lastname: Leitner
authors.affiliation: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Karpíšková
authors.affiliation: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Stibor
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, 166 28 Prague 6, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Slepička
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, 166 28 Prague 6, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Švorčík
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D002244:Carbon / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D020959:Polyethylene / Q000737:chemistry*; D017155:Wettability
chemical_list: D000588:Amines; D002244:Carbon; D020959:Polyethylene
keywords: Carbon nanoparticles; Cytocompatibility; Nanoparticles characterization; Surface amine functionalization; Surface characterization