title: [Whole Genome Expression Profiling Analysis of Metastasis and Drug-resistance-related Genes in Epithelial Ovarian Cancer Cells].
abstract: Objective To investigate the genes associated with higher ability of metastasis and chemotherapic resistance in epithelial ovarian carcinoma (EOC) by using Agilent whole genome oligonucleotide gene chip,with an attempt to further investigate the molecular mechanism of metastasis and chemotherapic resistance of EOC. Methods Oligonucleotide microarrays were used to determine whether gene expression profile might differentiate EOC cell lines (RMG-1-C,COC1 and HO8910) from their sub-lines (RMG-1-H,COC1/DDP and HO8910/PM) with higher ability of metastasis and chemotherapic resistance. Quantitative real-time polymerase chain reaction and immunohistochemical staining validated the microarray results. Results Gene expression profile identified 49 differentially expressed genes that showed≥2.0 fold change. All these differentially expressed genes were involved mainly in gene expression and biopolymer biosynthesis. Interaction network analysis predicted 21 genes participating in the regulatory connection. Highly differential expression of GCET2,CFTR,FOXP1 and GARS genes was validated by quantitative realtime polymerase chain reaction in all cell line samples,and the Results were consistent with microarray findings. Conclusion The change in the metastasis and chemotherapic resistance-associated gene expression profiles may provide a theoretical basis for studies on the molecular mechanisms of metastasis and chemotherapic resistance in EOC.
authors.affiliation: Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China.
authors.forename: Lian-cheng
authors.initials: LC
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China;Department of Obstetrics and Gynecology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China.
authors.forename: Juan-juan
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China.
authors.forename: Bei
authors.initials: B
authors.lastname: Lin
mesh_terms: D000077216:Carcinoma, Ovarian Epithelial; D019008:Drug Resistance, Neoplasm; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009362:Neoplasm Metastasis; D009375:Neoplasms, Glandular and Epithelial; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome