title: G-Quadruplexes Light up Localized DNA Circuits.
abstract: DNA circuits tethered to nanoplatforms can perform cascade reactions for signal amplification. One DNA single strand activates a strand-displacement cascade generating numerous outputs, and therefore amplifying the signal. These localized circuits present, however, an important limitation: the spontaneous activation of the cascade reaction. Current methods to stabilize these circuits employ combination of protective DNA strands, which need to be removed to activate the device. This protection-deprotection process generates an important amount of unwanted side reactions. This is indeed an important limitation for the large potential application of these amplification circuits. In the present work, G-quadruplex DNA structures were used to stabilize localized DNA circuits. This new protocol generates nanoplatforms that no longer requires protective-deprotective systems and is therefore completely neutral to the sample. In addition, cations such as Pb(2+) or Ca(2+) can be also employed to activate the device enlarging the potential applications from biosensors devices to metal detector sensors.
authors.affiliation: Université de Bordeaux , 33600 Bordeaux, France.|CBMN, CNRS UMR-5248 , F-33600 Pessac, France.
authors.forename: Oscar
authors.initials: O
authors.lastname: Mendoza
authors.affiliation: Université de Bordeaux , 33600 Bordeaux, France.|Inserm, U1212, CNRS, ARNA laboratory, IECB , F-33600 Pessac, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
authors.affiliation: Université de Bordeaux , 33600 Bordeaux, France.|CBMN, CNRS UMR-5248 , F-33600 Pessac, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Aimé
authors.affiliation: Université de Bordeaux , 33600 Bordeaux, France.|CBMN, CNRS UMR-5248 , F-33600 Pessac, France.
authors.forename: Juan
authors.initials: J
authors.lastname: Elezgaray
mesh_terms: D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry*; D054856:G-Quadruplexes; D008027:Light; D008670:Metals / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004277:DNA, Single-Stranded; D008670:Metals
keywords: DNA origami; DNA strand displacement; G-quadruplex; localized amplification circuits (LAC)