title: Metabolic differences of industrial acarbose-producing Actinoplanes sp. A56 under various osmolality levels.
abstract: Many investigations have revealed that a certain concentration of osmolality was indispensable for efficient acarbose production, but little information was available on the response mechanism of acarbose-producing strains to osmotic stress. By using the gas chromatography-mass spectrometry (GC-MS) analysis coupled with the enzyme activity determination of central carbon metabolism, the present work investigated the metabolic characteristics of industrial acarbose-producing Actinoplanes sp. A56 under various osmolality levels. Relatively high osmolality (450-500 mOsm/kg) appeared to favor efficient acarbose production by Actinoplanes sp. A56, although it inhibited cell growth. Further GC-MS analysis showed that fatty acids were the uppermost differential intracellular metabolites under various osmolality levels, and the relatively high osmolality resulted in increases in levels of fatty acids.
authors.affiliation: Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Kun-tai
authors.initials: KT
authors.lastname: Li
authors.affiliation: Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Wei-fu
authors.initials: WF
authors.lastname: Peng
authors.affiliation: Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China. xinch27@sina.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Cheng
mesh_terms: D020909:Acarbose / Q000378:metabolism*; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008320:Maltose / Q000378:metabolism; D008848:Micromonosporaceae / Q000254:growth & development / Q000378:metabolism*; D005810:Multigene Family; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D010100:Oxygen / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008320:Maltose; D002244:Carbon; D010100:Oxygen; D020909:Acarbose
keywords: Acarbose; Actinoplanes sp. A56; Metabolic response; Osmolality level