title: Microfluidics for cell-based high throughput screening platforms - A review.
abstract: In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As a major step in drug discovery, high-throughput screening allows rapid analysis of thousands of chemical, biochemical, genetic or pharmacological tests in parallel. In this review, we summarize the application of microfluidics in cell-based high throughput screening. The screening methods mentioned in this paper include approaches using the perfusion flow mode, the droplet mode, and the microarray mode. We also discuss the future development of microfluidic based high throughput screening platform for drug discovery.
authors.affiliation: Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China; Department of Mechanical Engineering, Materials Technology Institute, Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, 5600 MB, The Netherlands.
authors.forename: Guansheng
authors.initials: G
authors.lastname: Du
authors.affiliation: Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China. Electronic address: fangqun@zju.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Department of Mechanical Engineering, Materials Technology Institute, Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, 5600 MB, The Netherlands. Electronic address: j.m.j.d.toonder@tue.nl.
authors.forename: Jaap M J
authors.initials: JM
authors.lastname: den Toonder
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods*; D044085:Microfluidics
keywords: Cell-based microfluidics; Drug discovery; High throughput screening