title: Mechanical Bowel Preparation Does Not Affect Anastomosis Healing in an Experimental Rat Model.
abstract: BACKGROUND
  Mechanical bowel preparation before colorectal surgery is commonly performed, but its benefits are controversial. The aim of this study was to compare the effects of mechanical bowel preparation on healing of colonic anastomosis and tissue strength.
  
  
  METHODS
  After institutional review board approval, 20 adult Wistar albino rats were randomly divided into 2 groups of 10 animals each. Mechanical bowel preparation including sodium phosphate was performed on the experimental group via a feeding tube, whereas no bowel preparation procedures were performed on the control group. Transverse colon resection and anastomosis were performed on all rats under general anaesthesia. On postoperative day 5, re-laparotomy was performed and the anastomotic areas were resected. Animals were killed, after which bursting pressure and tissue hydroxyproline concentrations were measured, histopathological examination was performed, and we evaluated and compared the results.
  
  
  RESULTS
  There were no differences between control and experimental groups in bursting pressure, tissue hydroxyproline concentrations, or histopathological examination results (P>0.05).
  
  
  CONCLUSIONS
  Our study demonstrated no significant difference between bursting pressures, tissue hydroxyproline levels, or modified wound healing score at postoperative day 5 between rats undergoing and not undergoing mechanical bowel preparation. Mechanical bowel preparation is not essential for healing or strength of colonic anastomosis in rats.
authors.affiliation: Department of General Surgery, Bitlis State Hospital, Bitlis, Turkey.
authors.forename: Isılay
authors.initials: I
authors.lastname: Piroglu
authors.affiliation: Department of Anesthesiology & Reanimation, Pendik State Hospital, Istanbul, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Tulgar
authors.affiliation: Department of Pediatric Surgery, Pendik State Hospital, Istanbul, Turkey.
authors.forename: David Terence
authors.initials: DT
authors.lastname: Thomas
authors.affiliation: Department of Urology, Hisar Intercontinental Hospital, Istanbul, Turkey.
authors.forename: Basri
authors.initials: B
authors.lastname: Cakiroglu
authors.affiliation: Department of Anesthesiology & Reanimation, Bitlis State Hospital, Bitlis, Turkey.
authors.forename: Mustafa Devrim
authors.initials: MD
authors.lastname: Piroglu
authors.affiliation: Department of Emergency Medicine, Bitlis State Hospital, Bitlis, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Bozkurt
authors.affiliation: Department of Anesthesiology & Reanimation, Karakocan State Hospital, Elazig, Turkey.
authors.forename: Ruken
authors.initials: R
authors.lastname: Gergerli
authors.affiliation: Department of Anesthesiology & Reanimation, Gumushane State Hospital, Gumushane, Turkey.
authors.forename: Nagihan Gozde
authors.initials: NG
authors.lastname: Ates
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D002400:Cathartics / Q000008:administration & dosage*; D003082:Colectomy / Q000379:methods; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000737:chemistry; D010710:Phosphates / Q000008:administration & dosage*; D011184:Postoperative Period; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
chemical_list: D002400:Cathartics; D010710:Phosphates; D006909:Hydroxyproline; C018279:sodium phosphate