title: Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit.
abstract: Milk, as the sole source of nutrition for the newborn mammal, provides the necessary nutrients and energy for offspring growth and development. It also contains a vast number of bioactive compounds that greatly affect the development of the neonate. The analysis of milk components will help elucidate key factors that link maternal metabolism and health with offspring growth and development. The laboratory rat represents a popular model organism for maternal studies, and rat milk can be used to examine the effect of various maternal physiological, nutritional, and pharmacological interventions on milk components, which may then impact offspring health. Here a simple method of manually collecting milk from the lactating rat that can be performed by a single investigator, does not require specialized vacuum or suction equipment, and provides sufficient milk for subsequent downstream analysis is described. A method for estimating the fat content of milk by measuring the percentage of cream within the milk sample, known as the creamatocrit, is also presented. These methods can ultimately be used to increase insight into maternal-child health and to elucidate maternal factors that are involved in proper growth and development of offspring.
authors.affiliation: Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Paul
authors.affiliation: Faculty of Kinesiology, University of Calgary.
authors.forename: Megan C
authors.initials: MC
authors.lastname: Hallam
authors.affiliation: Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary; Faculty of Kinesiology, University of Calgary; reimer@ucalgary.ca.
authors.forename: Raylene A
authors.initials: RA
authors.lastname: Reimer
mesh_terms: D000818:Animals; D005223:Fats / Q000032:analysis; D005260:Female; D007774:Lactation / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000502:physiology*; D039382:Maternal Nutritional Physiological Phenomena; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis; D010121:Oxytocin / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D013048:Specimen Handling / Q000379:methods
chemical_list: D005223:Fats; D008894:Milk Proteins; D010121:Oxytocin