title: [Effect of the hydrophobin HFBI-fusion tag on exogenous protein accumulation in tobacco plant].
abstract: OBJECTIVE
  To explore the mechanisms by which HFBI fusions increase recombinant fusion protein accumulation in plants.
  
  
  METHODS
  The HFBI sequence from Trichoderma reesei was synthesized and two plant expression vectors for expression of green fluorescence protein (GFP) and GFP-HFBI were constructed. The vectors were inoculated in Nicotiana benthamiana plants through agroinfiltration, and the expression levels and mRNA accumulation levels of GFP in Nicotiana leaves were examined by Western blotting, ELISA and RT-PCR.
  
  
  RESULTS
  The HFBI fusion tag significantly enhanced the accumulation of GFP in the leaves of N. benthamiana without causing toxic effects. Endoplasmic reticulum-targeted GFP-HFBI fusion induced the formation of spherical protein particles in the plant cells.
  
  
  CONCLUSIONS
  HFBI fusions can increase the accumulation of its fusion partner in plants by forming stable protein particles, which probably shields the target protein from endogenous protease-induced degadation. HFBI fusion technology provides an alternative to improving recombinant protein expression in plants from agroinfection-compatible expression vectors.
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China, Yinchuan 750021, China. E-mail: 369534669@qq.com.
authors.forename: Xiqian
authors.initials: X
authors.lastname: Zhang
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Mu
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Ding
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Li
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
mesh_terms: D004721:Endoplasmic Reticulum; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D007093:Imidazoles / Q000737:chemistry*; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D005466:Fluorocarbons
chemical_list: D049452:Green Fluorescent Proteins; D007093:Imidazoles; D011993:Recombinant Fusion Proteins; C046634:1-(heptafluorobutyryl)imidazole; D005466:Fluorocarbons