title: Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury.
abstract: Radiation-induced lung injury (RILI) is a fatal condition featured by interstitial pneumonitis and fibrosis. Mesenchymal stem cells (MSCs) have been widely used for treating RILI in rodent models. In the present study, we aimed to investigate whether the therapeutic effects of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) on RILI were in a dose-dependent manner. A total of 100 mice were randomly divided into: a control group (n=25), subject to lung irradiation and injection of phosphate-buffered solution (PBS) via the tail vein; and the hBM-MSC group, subject to lung irradiation followed by injection of a low dose (1x103 hBM-MSCs/g), medium dose (5x103 hBM-MSCs/g) and high dose (1x104 hBM-MSCs/g) of hBM-MSCs in PBS through the tail vein, respectively. After sacrifice, the pulmonary tissues were subject to hematoxylin and eosin (H&E) staining, Masson's trichrome staining and immunohistochemical staining to investigate the pathological changes. Immunofluorescent staining was performed to evaluate the differentiation capacity of hBM-MSCs in vivo by analyzing the expression of SPC and PECAM. hBM-MSCs improved the survival rate and histopathological features in the irradiated mice, especially in the low-dose group. Marked decrease in collagen deposition was noted in the irradiated mice treated using a low dose of hBM-MSCs. In addition, hBM-MSCs attenuated secretion and expression of IL-10 and increased the expression of TNF-α. Furthermore, hBM-MSCs had the potential to differentiate into functional cells upon lung injury. Low-dose hBM-MSCs contributed to functional recovery in mice with RILI.
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Xia
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Chang
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Orthopaedics, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130000; Key Laboratory of Radiobiology (Chinese Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Dong
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D064593:Heterografts; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D011833:Radiation Injuries, Experimental / Q000150:complications / Q000601:surgery*; D017564:Radiation Pneumonitis / Q000601:surgery*; D060888:Real-Time Polymerase Chain Reaction