title: Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices.
abstract: A novel method (i.e., continuous magnetic cell separation in a microfluidic channel) is demonstrated to be capable of inducing multifractionation of mixed cell suspensions into multiple outlet fractions. Here, multicomponent cell separation is performed with three different distinguishable magnetic nanoclusters (MnFe2O4, Fe3O4, and CoFe2O4), which are tagged on A431 cells. Because of their mass magnetizations, which can be ideally altered by doping with magnetic atom compositions (Mn, Fe, and Co), the trajectories of cells with each magnetic nanocluster in a flow are shown to be distinct when dragged under the same external magnetic field; the rest of the magnetic characteristics of the nanoclusters are identically fixed. This proof of concept study, which utilizes the magnetization-controlled nanoclusters (NCs), suggests that precise and effective multifractionation is achievable with high-throughput and systematic accuracy for dynamic cell separation.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Byunghoon
authors.initials: B
authors.lastname: Kang
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Bumjoon
authors.initials: B
authors.lastname: Cha
authors.affiliation: Department of Materials Science and Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Bongsoo
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Seungmin
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Moo-Kwang
authors.initials: MK
authors.lastname: Shin
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Eunji
authors.initials: E
authors.lastname: Jang
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Hyun-Ouk
authors.initials: HO
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Seo Ryung
authors.initials: SR
authors.lastname: Bae
authors.affiliation: Department of Materials Science and Engineering, Pohang University of Science and Technology , 77 Cheongam-Ro, Nam-gu, Pohang 120-784, Korea.
authors.forename: Unyong
authors.initials: U
authors.lastname: Jeong
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Il
authors.initials: I
authors.lastname: Moon
authors.affiliation: Department of Radiology and Research Institute of Radiological Science, College of Medicine, Yonsei University , Seoul 120-752, South Korea.
authors.forename: Hye yeong
authors.initials: Hy
authors.lastname: Son
authors.affiliation: Department of Radiology and Research Institute of Radiological Science, College of Medicine, Yonsei University , Seoul 120-752, South Korea.
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea.
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D010316:Particle Size; D013499:Surface Properties; D028561:Transition Elements / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D028561:Transition Elements