title: In-vitro inhibitory effect of EGFL7-RNAi on endothelial angiogenesis in glioma.
abstract: OBJECTIVE
  To investigate the role and mechanism of epidermal growth factor like domain 7 (EGFL7) in glioma angiogenesis by cell co-culture and RNA interference.
  
  
  METHODS
  NSCs-HUVECs co-culture system was established using Transwell culturing techniques. The interactions between glioma and endothelial cells were simulated in-vitro. Cellular expression of EGFL7 in NSCs and HUVEC was targeted and suppressed by lentiviral vector carrying siRNA. The effect of EGFL7 on angiogenesis in glioma in-vitro micro-environment was detected by endothelial cell proliferation, adhesion and tube formation assay.
  
  
  RESULTS
  Following EGFL7 gene silencing, expression of EGFL7 in HUVECs was reduced and cell adhesion capability was inhibited significantly. Endothelial cells failed to form a lumen-like structure after EGFL7 gene silencing, shown by the tube formation assay.
  
  
  CONCLUSIONS
  By regulating endothelial cell adhesion, EGFL7 plays a key role in the regulation of glioma angiogenesis.
authors.affiliation: Department of Neurosurgery, Hainan Nongken Hospital Haikou, Hainan, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Neurology, Haikou Municipal People's Hospital Haikou, Hainan, China.
authors.forename: Ai-Yue
authors.initials: AY
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Hainan Nongken Hospital Haikou, Hainan, China.
authors.forename: Qiong-Guan
authors.initials: QG
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Hainan Nongken Hospital Haikou, Hainan, China.
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Hainan Nongken Hospital Haikou, Hainan, China.
authors.forename: Peng-Xiang
authors.initials: PX
authors.lastname: Xu
authors.affiliation: Department of Neurology, Haikou Municipal People's Hospital Haikou, Hainan, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Yu
mesh_terms: D002135:Calcium-Binding Proteins; D002448:Cell Adhesion; D049109:Cell Proliferation; D018920:Coculture Techniques; D066255:EGF Family of Proteins; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D000066449:Human Embryonic Stem Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference
chemical_list: D002135:Calcium-Binding Proteins; D066255:EGF Family of Proteins; C527877:EGFL7 protein, human; D016228:Endothelial Growth Factors
keywords: Epidermal growth factor like domain 7; RNA interference; angiogenesis