title: Characterization of bacterial isolates from the microbiota of mothers' breast milk and their infants.
abstract: This investigation assessed the potential of isolating novel probiotics from mothers and their infants. A subset of 21 isolates among 126 unique bacteria from breast milk and infant stools from 15 mother-infant pairs were examined for simulated GI transit survival, adherence to Caco-2 cells, bacteriocin production, and lack of antibiotic resistance. Of the 21 selected isolates a Lactobacillus crispatus isolate and 3 Lactobacillus gasseri isolates demonstrated good profiles of in vitro GI transit tolerance and Caco-2 cell adherence. Bacteriocin production was observed only by L. gasseri and Enterococcus faecalis isolates. Antibiotic resistance was widespread, although not universal, among isolates from infants. Highly similar isolates (≥ 97% similarity by barcode match) of Bifidobacterium longum subsp. infantis (1 match), Lactobacillus fermentum (2 matches), Lactobacillus gasseri (6 matches), and Enterococcus faecalis (1 match) were isolated from 5 infant-mother pairs. Antibiotic resistance profiles between these isolate matches were similar, except in one case where the L. gasseri isolate from the infant exhibited resistance to erythromycin and tetracycline, not observed in matching mother isolate. In a second case, L. gasseri isolates differed in resistance to ampicillin, chloramphenicol and vancomycin between the mother and infant. In this study, gram positive bacteria isolated from mothers' breast milk as well as their infants exhibited diversity in GI transit survival and acid inhibition of pathogens, but demonstrated limited ability to produce bacteriocins. Mothers and their infants offer the potential for identification of probiotics; however, even in the early stages of development, healthy infants contain isolates with antibiotic resistance.
authors.affiliation: a Global Microbiology Capability Organization; The Procter & Gamble Company ; Cincinnati , OH USA.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Kozak
authors.affiliation: a Global Microbiology Capability Organization; The Procter & Gamble Company ; Cincinnati , OH USA.
authors.forename: Duane
authors.initials: D
authors.lastname: Charbonneau
authors.affiliation: b Department of Food Bioprocessing and Nutrition Sciences ; North Carolina State University ; Raleigh , NC USA.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Sanozky-Dawes
authors.affiliation: b Department of Food Bioprocessing and Nutrition Sciences ; North Carolina State University ; Raleigh , NC USA.
authors.forename: Todd
authors.initials: T
authors.lastname: Klaenhammer
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000096:biosynthesis; D001644:Bifidobacterium / Q000302:isolation & purification; D018938:Caco-2 Cells; D005243:Feces / Q000382:microbiology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D007223:Infant; D007778:Lactobacillus / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D064307:Microbiota; D008895:Milk, Human / Q000382:microbiology*; D009035:Mothers; D019936:Probiotics
chemical_list: D001430:Bacteriocins
keywords: antibiotic resistance; bacterial; breast milk; fecal; infant; microbiota; probiotics