title: Identification of Notoginseng powder based on similarity to "DNA Barcoding Core-genotype".
abstract: Notoginseng powder is a medicine form of Notoginseng radix et rhizoma in Chinese Pharmacopeia. Common adulterants of Notoginseng powder appeared in the market include powder of Panax ginseng and Panax quinquefolius. Here, "DNA Barcoding Core-Genotype (DBCG)" based on ITS sequences was developed for authenticating Notoginseng powder. DBCG was the genotype with the highest frequency of DNA barcoding in species. The DBCG was found as the reference sequence and minimal-similarity to identify species was calculated by large sample analysis. Then we could calculate similarity index between DBCG and sequences of the unidentified Notoginseng powder and compare it through minimal-similarity to identify the species. This method was simple, efficient, and might be a preferable tool for accurate authentication.
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Song
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Guojie
authors.initials: G
authors.lastname: Xu
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: b Lishui Hospital of Tradition Chinese Medicine , Lishui , China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.affiliation: a School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Gu
mesh_terms: D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D005894:Panax / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D004365:Drugs, Chinese Herbal
keywords: DNA Barcoding Core-Genotype (DBCG); ITS sequence; Notoginseng powder; minimal-similarity