title: Caffeine augments the antidepressant-like activity of mianserin and agomelatine in forced swim and tail suspension tests in mice.
abstract: BACKGROUND
  The main goal of this research was an evaluation of the influence of caffeine on the activity of mianserin and agomelatine.
  
  
  METHODS
  The mouse forced swim test and tail suspension test were used to determine the influence of caffeine on the activity of the tested drugs. Drug concentrations in serum and brains were estimated by HPLC.
  
  
  RESULTS
  Caffeine increases the anti-immobility action of mianserin and agomelatine. The observed effects were not associated with changes in the level of drugs in serum or brains.
  
  
  CONCLUSIONS
  The synergistic effect of caffeine and the tested drugs may be associated with their summative actions on monoaminergic neurotransmission. Caffeine-mianserin and caffeine-agomelatine interactions might have been of pharmacodynamic origin.
authors.affiliation: Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland. Electronic address: ewa.poleszak@umlub.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Poleszak
authors.affiliation: Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szopa
authors.affiliation: Department of Pharmacokinetics and Physical Pharmacy, Collegium Medicum, Jagiellonian University, Kraków, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Wyska
authors.affiliation: Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland.
authors.forename: Wirginia
authors.initials: W
authors.lastname: Kukuła-Koch
authors.affiliation: Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Serefko
authors.affiliation: Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Wośko
authors.affiliation: Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Bogatko
authors.affiliation: Second Department of Gynecology, Medical University of Lublin, Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wróbel
authors.affiliation: Department of Animal Physiology, Institute of Biology and Biochemistry, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wlaź
mesh_terms: D000081:Acetamides / Q000008:administration & dosage*; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage*; D002110:Caffeine / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D019417:Hindlimb Suspension / Q000379:methods; D008297:Male; D008803:Mianserin / Q000008:administration & dosage*; D051379:Mice; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D013550:Swimming / Q000502:physiology / Q000523:psychology*; D009281:Naphthalenes
chemical_list: D000081:Acetamides; D000928:Antidepressive Agents; D002110:Caffeine; D008803:Mianserin; C084711:agomelatine; D009281:Naphthalenes
keywords: Antidepressant drugs; Caffeine; Forced swim test; Mice; Tail suspension test