title: Manganese-enhanced MR imaging of brain activation evoked by noxious peripheral electrical stimulation.
abstract: As imaging technology develops, magnetic resonance imaging (MRI) has furthered our understanding of brain function by clarifying the anatomical structure and generating functional imaging data related to information processing in pain conditions. Recent studies have reported that manganese (Mn(2+))-enhanced MRI (MEMRI) provides valuable information about the functions of the central nervous system. The aim of this study was to identify specific brain regions activated during noxious electric stimulation using high-resolution MEMRI. Male Sprague Dawley rats were divided into three groups: naïve, sham electrical stimulation, and noxious electric stimulation. Under urethane with α-chloralose mixture anesthesia, a catheter was placed in the external carotid artery to administrate 20% mannitol and manganese chloride (25mM MnCl2). Noxious electric stimulation (2Hz, 10V) was applied to the hind paw with a needle electrode. Stimulation-induced neuronal activation was detected using 4.7-T MRI. In response to noxious electrical stimulation, remarkable Mn(2+)-enhanced signals were observed in the agranular insular cortex, auditory cortex, primary somatosensory cortex of the hind limb, and granular and dysgranular insular cortex, which correspond to sensory tactile electric stimulus to the hindpaws. These results indicate that the combination of MEMRI with activity-induced Mn(2+)-dependent contrast can delineate functional areas in the rat brain.
authors.affiliation: Department of Physiology, Brain Korea PLUS Project for Medical Science, Brain Research Institute, Epilepsy Research Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
authors.forename: Myeounghoon
authors.initials: M
authors.lastname: Cha
authors.affiliation: Bioimaging Research Team, Korea Basic Science Institute, Ochang 28119, Republic of Korea.
authors.forename: Kyuhong
authors.initials: K
authors.lastname: Lee
authors.affiliation: Bioimaging Research Team, Korea Basic Science Institute, Ochang 28119, Republic of Korea.
authors.forename: Chulhyun
authors.initials: C
authors.lastname: Lee
authors.affiliation: Bioimaging Research Team, Korea Basic Science Institute, Ochang 28119, Republic of Korea.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Bioimaging Research Team, Korea Basic Science Institute, Ochang 28119, Republic of Korea.
authors.forename: Chaejoon
authors.initials: C
authors.lastname: Cheong
authors.affiliation: Department of Psychology, Chungnam National University, Daejeon 34134, Republic of Korea.
authors.forename: Jin-Hun
authors.initials: JH
authors.lastname: Sohn
authors.affiliation: Department of Physiology, Brain Korea PLUS Project for Medical Science, Brain Research Institute, Epilepsy Research Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. Electronic address: bhlee@yuhs.ac.
authors.forename: Bae Hwan
authors.initials: BH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D002712:Chlorides; D003287:Contrast Media; D004558:Electric Stimulation; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D017895:Manganese Compounds; D009474:Neurons / Q000502:physiology; D017207:Rats, Sprague-Dawley
chemical_list: D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds; C025340:manganese chloride
keywords: In vivo imaging; Manganese-enhanced MRI; Noxious electrical stimulation