title: Neuroprotective and antioxidant lanostanoid triterpenes from the fruiting bodies of Ganoderma atrum.
abstract: Five new lanostanoid triterpenes were isolated from the ethanol extract of the fruiting bodies of Ganoderma atrum. The structures of the isolated compounds were established based on 1D and 2D ((1)H-(1)H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated compounds were tested in vitro for neuroprotective activities against 6-OHDA-induced cell death in SH-SY5Y cells and radical scavenging activities. As a result, compounds 2 and 5 exhibited potent neuroprotective activity against 6-OHDA-induced cell death in SH-SY5Y cells with the lowest IC50 value (0.5 μM) while compounds 1, 3 and 4 possessed significant neuroprotective activity with IC50 value less than 10 μM. Additionally, all tested compounds 1-6 showed the comparable free radical scavenging activities with the standard drug trolox in both ABTS (+) and DPPH experiment.
authors.affiliation: Department of Internal Neurology, Benxi Central Hospital, Benxi 117000, PR China. Electronic address: qjm2118@163.com.
authors.forename: Junming
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Pharmaceutics, The 98th Hospital of PLA, Huzhou 313000, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Internal Neurology, Benxi Central Hospital, Benxi 117000, PR China. Electronic address: chengguang_song@sohu.com.
authors.forename: Chengguang
authors.initials: C
authors.lastname: Song
mesh_terms: D045744:Cell Line, Tumor / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D009447:Neuroblastoma / Q000473:pathology; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D014315:Triterpenes; C497083:lanostanoid; D007810:Lanosterol
keywords: Antioxidant activity; Ganoderma atrum; Ganodermataceae; Lanostanoid triterpenes; Neuroprotective activity