title: Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi Differentiation.
abstract: Adipose tissue stem cells (ADSCs) would be an attractive autologous cell source. However, ADSCs require invasive procedures, and has potential complications. Recently, urine stem cells (USCs) have been proposed as an alternative stem cell source. In this study, we compared USCs and ADSCs collected from the same patients on stem cell characteristics and capacity to differentiate into various cell lineages to provide a useful guideline for selecting the appropriate type of cell source for use in clinical application. The urine samples were collected via urethral catheterization, and adipose tissue was obtained from subcutaneous fat tissue during elective laparoscopic kidney surgery from the same patient (n = 10). Both cells were plated for primary culture. Cell proliferation, colony formation, cell surface markers, immune modulation, chromosome stability and multi-lineage differentiation were analyzed for each USCs and ADSCs at cell passage 3, 5, and 7. USCs showed high cell proliferation rate, enhanced colony forming ability, strong positive for stem cell markers expression, high efficiency for inhibition of immune cell activation compared to ADSCs at cell passage 3, 5, and 7. In chromosome stability analysis, both cells showed normal karyotype through all passages. In analysis of multi-lineage capability, USCs showed higher myogenic, neurogenic, and endogenic differentiation rate, and lower osteogenic, adipogenic, and chondrogenic differentiation rate compared to ADSCs. Therefore, we expect that USC can be an alternative autologous stem cell source for muscle, neuron and endothelial tissue reconstruction instead of ADSCs.
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu, Korea.
authors.forename: Hye Suk
authors.identifier: 0000-0001-7568-3211
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Seock Hwan
authors.identifier: 0000-0003-3796-2601
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Bum Soo
authors.identifier: 0000-0002-4873-3049
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Department of Urology, Yeungnam University College of Medicine, Daegu, Korea.
authors.forename: Jae Young
authors.identifier: 0000-0002-3611-3926
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Biomedical Science, Catholic University of Daegu, Gyeongsan, Korea.
authors.forename: Gang-Baek
authors.identifier: 0000-0001-6708-2256
authors.initials: GB
authors.lastname: Park
authors.affiliation: Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Tae Gyun
authors.identifier: 0000-0002-4390-0952
authors.initials: TG
authors.lastname: Kwon
authors.affiliation: BioMedical Research Institute, Kyungpook National University Hospital, Daegu, Korea.
authors.forename: So Young
authors.identifier: 0000-0003-4500-4956
authors.initials: SY
authors.lastname: Chun
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000637:transplantation; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D043171:Chromosomal Instability; D003114:Colony-Forming Units Assay; D006801:Humans; D007621:Karyotyping; D039902:Multipotent Stem Cells / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D050507:Subcutaneous Fat, Abdominal / Q000166:cytology*; D014182:Transplantation, Autologous; D014556:Urine / Q000166:cytology*
chemical_list: D015415:Biomarkers
keywords: ADSCs; Multi-lineage Differentiation; Passages; Stem Cell Characteristics; USCs