title: New monoclonal antibody-based test for Helicobacter pylori urease in gastric tissue.
abstract: To evaluate a new monoclonal antibody for Helicobacter pylori urease in gastric tissue.
  
  
  
  A total of 107 volunteers were enrolled. All subjects underwent a (13)C-urea breath test and esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia. Six biopsy specimens in the gastric antrum and body were obtained for a rapid urease test and histology. The new monoclonal antibody-based H. pylori urease test (HPU) was performed to rapidly and qualitatively detect urease in two biopsy specimens.
  
  
  
  H. pylori infection was diagnosed in 73 subjects. The sensitivity and specificity of the HPU was 89% and 74%, respectively. The subjects were divided into two groups: one with true-positive and true-negative HPU results (n = 90) and the other with false-positive and false-negative HPU results (n = 17). Across all subjects, ammonia levels were 900.5 ± 646.7 and 604.3 ± 594.3 μmol/L (p > 0.05), and pH was 3.37 ± 1.64 and 2.82 ± 1.51 (p > 0.05). Sensitivity was higher in the presence of atrophic gastritis or intestinal metaplasia.
  
  
  
  HPU detected H. pylori in approximately 10 min. Gastric aspirate ammonia and pH levels did not affect the test results. Sensitivity was good in the presence of atrophic gastritis or intestinal metaplasia.
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Ho Dong
authors.initials: HD
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Hyeuk
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Seung
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Beom
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Woo Jong
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Chang Kook
authors.initials: CK
authors.lastname: Park
authors.affiliation: Department of Pathology, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Young Jik
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Pathology, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Pathology, Saint Carollo Hospital, Suncheon, Korea.
authors.forename: Hyung Rag
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Department of Clinical Pathology, Gwang Yang Health College, Gwangyang, Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Joo
authors.affiliation: Department of Biochemistry, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Young Do
authors.initials: YD
authors.lastname: Jung
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005757:Gastritis, Atrophic / Q000175:diagnosis* / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D011237:Predictive Value of Tests; D011706:Pyloric Antrum / Q000382:microbiology* / Q000473:pathology; D015203:Reproducibility of Results; D013997:Time Factors; D014510:Urease / Q000032:analysis* / Q000276:immunology; D057188:Workflow
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D015415:Biomarkers; D014510:Urease
keywords: Helicobacter pylori; Monoclonal antibody-based test; Urease