title: Ncl Synchronously Regulates Na+, K+, and Cl- in Soybean and Greatly Increases the Grain Yield in Saline Field Conditions.
abstract: Salt stress inhibits soybean growth and reduces gain yield. Genetic improvement of salt tolerance is essential for sustainable soybean production in saline areas. In this study, we isolated a gene (Ncl) that could synchronously regulate the transport and accumulation of Na(+), K(+), and Cl(-) from a Brazilian soybean cultivar FT-Abyara using map-based cloning strategy. Higher expression of the salt tolerance gene Ncl in the root resulted in lower accumulations of Na(+), K(+), and Cl(-) in the shoot under salt stress. Transfer of Ncl with the Agrobacterium-mediated transformation method into a soybean cultivar Kariyutaka significantly enhanced its salt tolerance. Introgression of the tolerance allele into soybean cultivar Jackson, using DNA marker-assisted selection (MAS), produced an improved salt tolerance line. Ncl could increase soybean grain yield by 3.6-5.5 times in saline field conditions. Using Ncl in soybean breeding through gene transfer or MAS would contribute to sustainable soybean production in saline-prone areas.
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.|Cuulong Delta Rice Research Institute (CLRRI), Thoilai, Cantho, Vietnam.
authors.forename: Tuyen Duc
authors.initials: TD
authors.lastname: Do
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.|Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, China.
authors.forename: Huatao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.|Genetic Engineering Division, Agricultural Genetics Institute, Hanoi, Vietnam.
authors.forename: Vu Thi Thu
authors.initials: VT
authors.lastname: Hien
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.|International Center for Agricultural Research in the Dry Areas (ICARDA), Cairo, Egypt.
authors.forename: Aladdin
authors.initials: A
authors.lastname: Hamwieh
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Institute of Crop Germplasm Resources, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Institute of Crop Germplasm Resources, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Cong
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Shono
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suenaga
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan.
authors.forename: Donghe
authors.initials: D
authors.lastname: Xu
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D002523:Edible Grain / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D028021:Sodium-Potassium-Chloride Symporters / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014170:Transformation, Genetic; D019076:Transgenes
chemical_list: D010940:Plant Proteins; D028021:Sodium-Potassium-Chloride Symporters