title: High-efficiency biosynthesis of hypocrellin A in Shiraia sp. using gamma-ray mutagenesis.
abstract: Hypocrellin A (HA), well known as one of the best natural pigments and bioactive agent to treat skin diseases, is further anticipated to play a vital role in photodynamic therapy (PDT) in anticancer and antiviral treatments. In this study, an HA-producing strain ZZZ816 (Shiraia sp.) was isolated from the moso bamboo (Phyllostachys edulis) seeds, and gamma irradiation was used to mutagenize spores of the original strain. After treatment with cobalt-60 gamma ((60)Coγ) with different doses (20, 50, 80, 100, 150, 180, 300, and 500 Gy), the 100 Gy was selected as the optimal condition, which led to 77.2 % lethality of spores and 35 % positive mutant frequency. The extracted compound of the most excellent HA-producing strain (H-4-2) was precisely analyzed by a combination of seven detection methods, and the maximum HA content was shown to reach 2018.3 mg/L. HA production in H-4-2 increased by 414.9 % compared to that of original strain ZZZ816 (392 mg/L) and was significantly higher than all the other industrial HA-producing strains in published reports.
authors.affiliation: College of Life Science, Capital Normal University, Beijing, 100048, People's Republic of China.
authors.forename: Xin-Yao
authors.initials: XY
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing, 100048, People's Republic of China. shenxiaoye2008@gmail.com.
authors.forename: Xiao-Ye
authors.initials: XY
authors.lastname: Shen
authors.affiliation: College of Life Science, Capital Normal University, Beijing, 100048, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the SFA, International Centre for Bamboo and Rattan, Beijing, 100102, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Life Science, Capital Normal University, Beijing, 100048, People's Republic of China. houchenglincn@yahoo.com.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Hou
mesh_terms: D001203:Ascomycota / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D007218:Industrial Microbiology; D016296:Mutagenesis; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D019800:Phenol; D011809:Quinones / Q000378:metabolism*; D031787:Sasa / Q000382:microbiology; D012639:Seeds / Q000382:microbiology
chemical_list: D011809:Quinones; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A
keywords: Biosynthesis; Gamma-ray mutagenesis; Genetic stability; Hypocrellin A; Production; Shiraia sp.