title: CREB/GSK-3β signaling pathway regulates the expression of TR4 orphan nuclear receptor gene.
abstract: In this study, we show that reduction of glucose concentration increases TR4 expression in 3T3-L1 cells via stimulation of the GSK-3β-CREB pathway. While GSK-3β and CREB increased TR4 expression in 3T3-L1 cells, inhibition of CREB expression or activity resulted in loss of GSK-3β-mediated enhancement of TR4 expression. In addition, CREB enhanced murine TR4 promoter activity via direct binding to a cAMP response element located in the promoter, and this CREB effect was further strengthened by GSK-3β. Moreover, silencing of TR4 expression by a gene-specific microRNA inhibited CREB-induced lipid accumulation in 3T3-L1 adipocytes, suggesting that TR4 could be a key mediator of CREB-induced lipogenesis.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, South Korea.
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, South Korea.
authors.forename: Hojung
authors.initials: H
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, South Korea.
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: George Whipple Laboratory for Cancer Research, Departments of Pathology, Urology and Radiation Oncology, and Caner Center, University of Rochester Medical Center, Rochester, NY, USA.
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, South Korea. Electronic address: ekim@chonnam.ac.kr.
authors.forename: Eungseok
authors.initials: E
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology*; D004734:Energy Metabolism; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000502:physiology*; D000071679:Glycogen Synthase Kinase 3 beta; D057809:HEK293 Cells; D006706:Homeostasis; D006801:Humans; D051379:Mice; D011485:Protein Binding; D020218:Response Elements; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3; D005947:Glucose
keywords: 3T3-L1; CREB; GSK-3β; Lipogenesis; TR4