title: Cyclodextrin-poloxamer aggregates as nanocarriers in eye drop formulations: dexamethasone and amphotericin B.
abstract: In this present study cyclodextrin (CD)-poloxamer aggregates were characterized and developed as ophthalmic drug carriers. The combined effect of γCD/2-hydroxypropyl-γCD (HPγCD) mixtures and poloxamer on solubilization and permeability of two model drugs, dexamethasone (Dex) and amphotericin B (AmB), was investigated. The CD-poloxamer interaction and complex aggregation were examined by (1)H nuclear magnetic resonance ((1)H-NMR), their solubilizing ability by high-performance liquid chromatography, and their particle size determined by dynamic light scattering and transmission electron microscopy. Formulations containing either 1.5% w/v Dex or 0.15% w/v AmB in eye drop suspensions containing various γCD/HPγCD ratios and poloxamer 407 (P407) were prepared. The solubility of the drugs, surface tension and hemolytic effect of the eye drops and drug permeation from selected formulations were determined. The (1)H-NMR study showed that P407 formed inclusion complex with CDs by inserting its poly(propylene oxide) segment into the CD cavity. P407 and γCD interacted with each other to form nanosized aggregates, and the observed concentration of dissolved γCD and P407 progressively decreased with increasing γCD and P407 concentrations. Including a high proportion of HPγCD improved the drug solubilization and reduced the hemolytic effect. The surface tension of the formulations decreased with increasing P407 concentration. Furthermore, increasing P407 content in the formulations enhanced formation of complex aggregates with consequent slower drug release. It was concluded that the drug/γCD/HPγCD complex was stabilized by P407 through formation of multi-component aggregates. Thus, CD-poloxamer aggregates are self-assembled nanocarriers from which drug delivery characteristics can be adjusted by changing the γCD/HPγCD/P407 ratios.
authors.affiliation: a Faculty of Pharmaceutical Sciences , Chulalongkorn University , Pathumwan, Bangkok , Thailand ;
authors.forename: Phatsawee
authors.initials: P
authors.lastname: Jansook
authors.affiliation: b Faculty of Pharmaceutical Sciences , Prince of Songkla University , Songkhla , Thailand ;
authors.forename: Wiwat
authors.initials: W
authors.lastname: Pichayakorn
authors.affiliation: c Faculty of Pharmaceutical Sciences , University of Iceland , Reykjavik , Iceland.
authors.forename: Chutimon
authors.initials: C
authors.lastname: Muankaew
authors.affiliation: c Faculty of Pharmaceutical Sciences , University of Iceland , Reykjavik , Iceland.
authors.forename: Thorsteinn
authors.initials: T
authors.lastname: Loftsson
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry*; D003907:Dexamethasone / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D009883:Ophthalmic Solutions / Q000737:chemistry*; D010316:Particle Size; D010539:Permeability; D020442:Poloxamer / Q000737:chemistry*; D012995:Solubility; D013535:Suspensions / Q000737:chemistry; D047408:gamma-Cyclodextrins / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D004337:Drug Carriers; D009883:Ophthalmic Solutions; D013535:Suspensions; D047408:gamma-Cyclodextrins; D020442:Poloxamer; D003907:Dexamethasone; D000666:Amphotericin B
keywords: Aggregate; amphotericin B; cyclodextrin; dexamethasone; eye drop; poloxamer