title: Biological hydrogen and methane production from bagasse bioethanol fermentation residues using a two-stage bioprocess.
abstract: This study investigated the recovery of H2 and CH4 from bagasse bioethanol fermentation residues (bagasse BEFR) using a two-stage bioprocess. In the hydrogen fermentation bioreactor (HFB), carbohydrate removal efficiency was maintained at 82-93% and the highest hydrogen yield was 8.24mL/gCOD at volumetric loading rate (VLR) of 80kgCOD/m(3)/day. The results indicated a positive correlation between hydrogen yield and butyrate-to-acetate ratio, which might be due to the mechanisms of lactate/acetate utilization for hydrogen production and acetogenesis occurring in the HFB. Remaining volatile fatty acids and alcohols in the HFB effluent were further utilized for methane production in methane fermentation bioreactor (MFB), in which the highest methane yield of 345.2mL/gCOD was attained at VLR of 2.5kgCOD/m(3)/day. Overall, the two-stage bioprocess achieved a maximum COD removal of 81% from bagasse BEFR, and converted 0.3% and 72.8% of COD in the forms of H2 and CH4, respectively.
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701, Taiwan.
authors.forename: Hai-Hsuan
authors.initials: HH
authors.lastname: Cheng
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701, Taiwan; Sustainable Environment Research Laboratory (SERL), National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy (RCETS), National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701, Taiwan. Electronic address: whang@mail.ncku.edu.tw.
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Whang
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701, Taiwan.
authors.forename: Man-Chien
authors.initials: MC
authors.lastname: Chung
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University (NCKU), No. 1, University Road, Tainan 701, Taiwan.
authors.forename: Kun-Chi
authors.initials: KC
authors.lastname: Chan
mesh_terms: D000085:Acetates / Q000032:analysis; D056804:Biofuels / Q000032:analysis; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002087:Butyrates / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis; D002482:Cellulose / Q000737:chemistry*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000032:analysis*; D008697:Methane / Q000096:biosynthesis*; D062065:Wastewater / Q000737:chemistry; D014994:Xylose / Q000032:analysis
chemical_list: D000085:Acetates; D056804:Biofuels; D002087:Butyrates; D002264:Carboxylic Acids; D002482:Cellulose; D000431:Ethanol; D006859:Hydrogen; D008697:Methane; D062065:Wastewater; D014994:Xylose; C027433:bagasse
keywords: Acetogenesis; Anaerobic wastewater treatment; B/A ratio; Bioenergy