title: Proteome, Allergenome, and Novel Allergens of House Dust Mite, Dermatophagoides farinae.
abstract: Dermatophagoides farinae mite is a predominant source of indoor allergens causing high incidence of allergy worldwide. People with different genetic background respond differently to the mite components, and thus the component-resolved diagnosis (CRD) is preferred to the conventional allergy test based on crude mite extract. In this study, proteome and culprit components in the D. farinae whole body extract that sensitized the allergic patients were studied by using SDS-PAGE (1DE) and 2DE-IgE immunoblotting followed by LC-MS/MS and database search for protein identification. From the 1DE, the mite extract revealed 105 proteins that could be classified into seven functionally different groups: allergens, structural components, enzymes, enzyme inhibitor, receptor proteins, transporters, and binding/regulatory/cell signaling proteins. From the 2DE, the mite extract produced 94 spots; 63 were bound by IgE in sera of 20 D. farinae allergic patients. One more protein that was not revealed by the 2DE and protein staining reacted with IgE in 2 allergic patients. Proteins in 40 spots could be identified as 35 different types. Three of them reacted to IgE of >50% of the allergic patients, and hence they are major allergens: tropomyosin or Der f 10 (75%), aconitate hydratase (70%), and one uncharacterized protein (55%). Aconitate hydratase is a novel D. farinae major allergen unraveled in this study. Several mite minor allergens that have never been previously reported are also identified. The data have clinical applications in the component-resolved diagnosis for tailor-designed allergen-specific immunotherapy.
authors.affiliation: Graduate Program in Microbiology, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.|Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
authors.forename: Jintarat
authors.initials: J
authors.lastname: Choopong
authors.affiliation: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University , Bangkok 10400, Thailand.
authors.forename: Onrapak
authors.initials: O
authors.lastname: Reamtong
authors.affiliation: Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
authors.forename: Nitat
authors.initials: N
authors.lastname: Sookrung
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
authors.forename: Watee
authors.initials: W
authors.lastname: Seesuay
authors.affiliation: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University , Bangkok 10400, Thailand.
authors.forename: Nitaya
authors.initials: N
authors.lastname: Indrawattana
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
authors.forename: Yuwaporn
authors.initials: Y
authors.lastname: Sakolvaree
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
authors.forename: Wanpen
authors.initials: W
authors.lastname: Chaicumpa
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 10700, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tungtrongchitr
mesh_terms: D000485:Allergens / Q000378:metabolism*; D000818:Animals; D060829:Arthropod Proteins / Q000145:classification / Q000378:metabolism*; D002853:Chromatography, Liquid; D030562:Databases, Protein; D040001:Dermatophagoides farinae / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000469:parasitology; D015151:Immunoblotting; D020543:Proteome / Q000145:classification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D000485:Allergens; D060829:Arthropod Proteins; D020543:Proteome
keywords: Dermatophagoides farinae; IgE; LC−ESI−MS/MS; allergen; allergenome; proteome