title: Extracellular Polymeric Substances of Aphanizomenon flos-aquae (EPS-A) Induced Apoptosis in Astrocytes of Zebrafish.
abstract: In this study, extracellular polymeric substances of Aphanizomenon flos-aquae (EPS-A) were investigated in order to explore their effect on astrocytes of zebrafish and potential risk for environment. Astrocytes were treated with varying concentrations of EPS-A, the results showed that EPS-A inhibited astrocytes growth in a dose-and time-dependent manner. With the concentrations of EPS-A increasing, the adherent ability of astrocytes decreased and the number of astrocytes floating in the culture medium increased. When treated with 2.35 µg/mL EPS-A, EPS-A induced cell cycle arrest and made the collapse of mitochondrial membrane potential and then led to astrocytes apoptosis. The results suggested that EPS-A could pose a threat to zebrafish and represent risk for environment, so regularly monitoring the presence of EPS-A was very important in nutrient-rich freshwaters when A. flos-aquae blooms broke out.
authors.affiliation: School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Anesthesiology, Gansu Provincial Maternity and Child-Care Hospital, Lanzhou, 730050, Gansu, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Xue
authors.affiliation: School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tao
authors.affiliation: Department of Life Science and Biotechnology, College of Science, Wuhan University of Technology, Wuhan, 430070, Hubei, China.
authors.forename: Delu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, Hubei, China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Hu
authors.affiliation: School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China. renjunjd@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
mesh_terms: D000818:Animals; D046890:Aphanizomenon / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000187:drug effects* / Q000473:pathology; D001704:Biopolymers / Q000302:isolation & purification / Q000633:toxicity*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water / Q000737:chemistry*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D001704:Biopolymers; D014874:Water Pollutants, Chemical
keywords: Aphanizomenon flos-aquae; Apoptosis; Astrocytes; Cyanobacteria blooms; Extracellular polymeric substances; Zebrafish