title: High-performance liquid chromatographic method for profiling 2-oxo acids in urine and its application in evaluating vitamin status in rats.
abstract: B-group vitamins are involved in the catabolism of 2-oxo acids. To identify the functional biomarkers of B-group vitamins, we developed a high-performance liquid chromatographic method for profiling 2-oxo acids in urine and applied this method to urine samples from rats deficient in vitamins B1 and B6 and pantothenic acid. 2-Oxo acids were reacted with 1,2-diamino-4,5-methylenebenzene to produce fluorescent derivatives, which were then separated using a TSKgel ODS-80Ts column with 30 mmol/L of KH2PO4 (pH 3.0):acetonitrile (7:3) at a flow rate of 1.0 mL/min. Vitamin B1 deficiency increased urinary levels of all 2-oxo acids, while vitamin B6 deficiency only increased levels of sum of 2-oxaloacetic acid and pyruvic acid, and pantothenic acid deficiency only increased levels of 2-oxoisovaleric acid. Profiles of 2-oxo acids in urine samples might be a non-invasive way of clarifying the functional biomarker of B-group vitamins.
authors.affiliation: a Department of Nutrition, School of Human Cultures , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Shibata
authors.affiliation: a Department of Nutrition, School of Human Cultures , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Chifumi
authors.initials: C
authors.lastname: Nakata
authors.affiliation: a Department of Nutrition, School of Human Cultures , University of Shiga Prefecture , Hikone , Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fukuwatari
mesh_terms: D000272:Adipates / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D045782:Hemiterpenes; D007651:Keto Acids / Q000652:urine; D007656:Ketoglutaric Acids / Q000652:urine; D008297:Male; D062907:Oxaloacetic Acid / Q000652:urine; D010205:Pantothenic Acid / Q000172:deficiency / Q000652:urine*; D010655:Phenylenediamines / Q000737:chemistry; D019289:Pyruvic Acid / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D013831:Thiamine / Q000652:urine*; D013832:Thiamine Deficiency / Q000652:urine*; D025101:Vitamin B 6 / Q000652:urine*; D026681:Vitamin B 6 Deficiency / Q000652:urine*
chemical_list: D000272:Adipates; D015415:Biomarkers; D045782:Hemiterpenes; D007651:Keto Acids; D007656:Ketoglutaric Acids; D010655:Phenylenediamines; D010205:Pantothenic Acid; D062907:Oxaloacetic Acid; C001505:alpha-ketoisovalerate; D025101:Vitamin B 6; C013082:alpha-ketoisocaproic acid; D019289:Pyruvic Acid; C001918:alpha-ketoadipic acid; D013831:Thiamine
keywords: 2-oxo acid-gram; B-group vitamins; functional biomarker; nutritional status; urine 2-oxo acid