title: EBNA3C Directs Recruitment of RBPJ (CBF1) to Chromatin during the Process of Gene Repression in EBV Infected B Cells.
abstract: It is well established that Epstein-Barr virus nuclear antigen 3C (EBNA3C) can act as a potent repressor of gene expression, but little is known about the sequence of events occurring during the repression process. To explore further the role of EBNA3C in gene repression-particularly in relation to histone modifications and cell factors involved-the three host genes previously reported as most robustly repressed by EBNA3C were investigated. COBLL1, a gene of unknown function, is regulated by EBNA3C alone and the two co-regulated disintegrin/metalloproteases, ADAM28 and ADAMDEC1 have been described previously as targets of both EBNA3A and EBNA3C. For the first time, EBNA3C was here shown to be the main regulator of all three genes early after infection of primary B cells. Using various EBV-recombinants, repression over orders of magnitude was seen only when EBNA3C was expressed. Unexpectedly, full repression was not achieved until 30 days after infection. This was accurately reproduced in established LCLs carrying EBV-recombinants conditional for EBNA3C function, demonstrating the utility of the conditional system to replicate events early after infection. Using this system, detailed chromatin immunoprecipitation analysis revealed that the initial repression was associated with loss of activation-associated histone modifications (H3K9ac, H3K27ac and H3K4me3) and was independent of recruitment of polycomb proteins and deposition of the repressive H3K27me3 modification, which were only observed later in repression. Most remarkable, and in contrast to current models of RBPJ in repression, was the observation that this DNA-binding factor accumulated at the EBNA3C-binding sites only when EBNA3C was functional. Transient reporter assays indicated that repression of these genes was dependent on the interaction between EBNA3C and RBPJ. This was confirmed with a novel EBV-recombinant encoding a mutant of EBNA3C unable to bind RBPJ, by showing this virus was incapable of repressing COBLL1 or ADAM28/ADAMDEC1 in newly infected primary B cells.
authors.affiliation: Molecular Virology, Department of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Jens S
authors.initials: JS
authors.lastname: Kalchschmidt
authors.affiliation: Molecular Virology, Department of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Adam C T
authors.initials: AC
authors.lastname: Gillman
authors.affiliation: Molecular Virology, Department of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Kostas
authors.initials: K
authors.lastname: Paschos
authors.affiliation: Molecular Virology, Department of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Bazot
authors.affiliation: Department of Gene Vectors, Hematologikum, Helmholtz Zentrum München, National Research Center for Environmental Health, München, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Kempkes
authors.affiliation: Molecular Virology, Department of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Allday
mesh_terms: D001402:B-Lymphocytes / Q000821:virology*; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D006790:Host-Parasite Interactions / Q000235:genetics; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002843:Chromatin; C498774:EBNA-3C, epstein-barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; C497570:RBPJ protein, human