title: Identification of sheep red blood cell (SRBC) surface immune-responsive peptides detected by antisera from SRBC-immunized rats.
abstract: To identify the sheep red blood cell (SRBC) surface immune-responsive peptides, immuno-reactive fraction of SRBC was detected by SDS-PAGE and western blot analysis with antisera from SRBC-immunized rats. Then the most intense immuno-reactive band on SDS-PAGE was subjected to nanoLC-ESI-MS/MS analysis, and 17 proteins were identified including membrane proteins of erythrocytes such as band 3 anion transport protein isoform 1 (Anion exchange protein 1; AE-1, CD233), Ammonium transporter Rh type A (Rh type A glycoprotein, CD241) and Ankyrin-1 (ANK-1), Spectrin beta chain. Among them, plasma protein AE-1 (CD233) and Rh type A glycoprotein (CD241) have transmembrane domain and correspond to extracellular region in their sequences. These extracellular regions of the plasma membrane proteins are supposed to be major immune-responsive peptides of SRBC in rats. These peptides are promising for the construction of an ELISA system which does not require the processing of SRBC membrane ghosts.
authors.affiliation: Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takeyoshi
mesh_terms: D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology / Q000302:isolation & purification; D017487:Ankyrins / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D012756:Sheep; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; C540195:Ank1 protein, rat; D017487:Ankyrins; D007106:Immune Sera; D010455:Peptides