title: miR-193a-3p regulation of chemoradiation resistance in oesophageal cancer cells via the PSEN1 gene.
abstract: Chemoradiation therapy is an important component of the curative treatment for oesophageal carcinomas. These therapeutic effects are prevented in patients according to radioresistance and multi-drug resistance, and the cause of such resistance remains unclear. In this study, we identified the role of miR-193a-3p in modulating the radioresistance and chemoresistance of oesophageal cancer cells. We found that KYSE150 and KYSE410 cells could be characterized as relatively radiation-sensitive and radiation-resistant cells, respectively. Similarly, KYSE150 and KYSE410 cells were found to be chemosensitive and chemoresistant, respectively. Over-expression of miR-193a-3p increased the radioresistance and chemoresistance of oesophageal squamous cell carcinoma (ESCC) cells. In contrast, the down-regulation of miR-193a-3p decreased the radioresistance and chemoresistance of ESCC cells. In addition, miR-193a-3p inducing DNA damage has also been demonstrated through measuring the level of gamma-H2AX associated with miR-193a-3p. Moreover, a small interfering RNA(siRNA)-induced repression of the PSEN1 gene had an effect similar to that of miR-193a-3p up-regulation. The above processes also inhibited oesophageal cancer cells apoptosis. These findings suggest that miR-193a-3p contributes to the radiation and chemotherapy resistance of oesophageal carcinoma by down-regulating PSEN1. Thus, miR-193a-3p and PSEN1 might be potential biomarkers for chemoradiation resistant cancers.
authors.affiliation: Department of Radiotherapy, Anhui Cancer Hospital, Hefei, Anhui 230031, China. Electronic address: 1665045793@qq.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of Radiotherapy, Anhui Cancer Hospital, Hefei, Anhui 230031, China. Electronic address: money2004@sina.com.
authors.forename: Liting
authors.initials: L
authors.lastname: Qian
authors.affiliation: Cancer Epigenetics Program, Anhui Cancer Hospital, Hefei, Anhui 230031, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Radiotherapy physics, Anhui Cancer Hospital, Hefei, Anhui 230031, China.
authors.forename: Bojin
authors.initials: B
authors.lastname: Ding
authors.affiliation: Department of Radiotherapy physics, Anhui Cancer Hospital, Hefei, Anhui 230031, China.
authors.forename: Gieping
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Radiotherapy, Anhui Cancer Hospital, Hefei, Anhui 230031, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Urology, Anhui Provincial Hospital, China.
authors.forename: Sanqiang
authors.initials: S
authors.lastname: Niu
authors.affiliation: Department of Biology, School of Life Science, Anhui Medical University, Hefei, Anhui 230031, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D059248:Chemoradiotherapy; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000532:radiotherapy; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D053764:Presenilin-1 / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C546818:MIRN193 microRNA, human; D035683:MicroRNAs; C508853:PSEN1 protein, human; D053764:Presenilin-1
keywords: ESCC; PSEN1; Radioresistance; X-ray; miR-193a-3p; r-H2AX