title: Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting.
abstract: The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 ± 3.27 to 75.29 ± 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model.
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea. kimins21@kirams.re.kr.
authors.forename: Min Sup
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea. sjpark@kirams.re.kr.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea. whgnlans@hanmail.net.
authors.forename: Wheemoon
authors.initials: W
authors.lastname: Cho
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea. bons1020@kirams.re.kr.
authors.forename: Bon Kang
authors.initials: BK
authors.lastname: Gu
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea. chkim@kcch.re.kr.
authors.forename: Chun-Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013373:Substance P / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D018377:Neurotransmitter Agents; D013373:Substance P; D048271:Chitosan
keywords: chitosan; electrospinning; human mesenchymal stem cell; substance-P; tissue engineering; wound healing