title: Genetic linkage of capsid protein-encoding RNA segments in group A equine rotaviruses.
abstract: Rotavirus virions are formed by three concentric protein layers that enclose the 11 dsRNA genome segments and the viral proteins VP1 and VP3. Interactions amongst the capsid proteins (VP2, VP6, VP7 and VP4) have been described to play a major role in viral fitness, whilst restricting the reassortment of the genomic segments during co-infection with different rotavirus strains. In this work we describe and characterize the linkage between VP6 and VP7 proteins based on structural and genomic analyses of group A rotavirus strains circulating in Argentinean horses. Strains with the VP7 genotype G3 showed a strong association with the VP6 genotype I6, whilst strains with G14 were associated with the I2 genotype. Most of the differences on the VP6 and VP7 proteins were observed in interactive regions between the two proteins, suggesting that VP6 : VP7 interactions may drive the co-evolution and co-segregation of their respective gene segments.
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Nicolás Repetto y De los Reseros s/n (CP 1816), Hurlingham, Buenos Aires, Argentina.
authors.forename: Samuel
authors.initials: S
authors.lastname: Miño
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Nicolás Repetto y De los Reseros s/n (CP 1816), Hurlingham, Buenos Aires, Argentina.|Escuela de Veterinaria, Universidad del Salvador, Champagnat 1599, Ruta Panamericana km54.5 (B1630AHU), Pilar, Buenos Aires, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: Barrandeguy
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Nicolás Repetto y De los Reseros s/n (CP 1816), Hurlingham, Buenos Aires, Argentina.
authors.forename: Viviana
authors.initials: V
authors.lastname: Parreño
authors.affiliation: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors.forename: Gabriel I
authors.initials: GI
authors.lastname: Parra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005075:Biological Evolution; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000821:virology; D008040:Genetic Linkage; D016679:Genome, Viral; D005838:Genotype; D006734:Horse Diseases / Q000821:virology; D006736:Horses; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C069187:VP1 protein, Rotavirus; C442546:VP3 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D014758:Viral Core Proteins