title: Fine genetic mapping of spot blotch resistance gene Sb3 in wheat (Triticum aestivum).
abstract: Spot blotch disease resistance gene Sb3 was mapped to a 0.15 centimorgan (cM) genetic interval spanning a 602 kb physical genomic region on chromosome 3BS. Wheat spot blotch disease, caused by B. sorokiniana, is a devastating disease that can cause severe yield losses. Although inoculum levels can be reduced by planting disease-free seed, treatment of plants with fungicides and crop rotation, genetic resistance is likely to be a robust, economical and environmentally friendly tool in the control of spot blotch. The winter wheat line 621-7-1 confers immune resistance against B. sorokiniana. Genetic analysis indicates that the spot blotch resistance of 621-7-1 is controlled by a single dominant gene, provisionally designated Sb3. Bulked segregant analysis (BSA) and simple sequence repeat (SSR) mapping showed that Sb3 is located on chromosome arm 3BS linked with markers Xbarc133 and Xbarc147. Seven and twelve new polymorphic markers were developed from the Chinese Spring 3BS shotgun survey sequence contigs and 3BS reference sequences, respectively. Finally, Sb3 was mapped in a 0.15 cM genetic interval spanning a 602 kb physical genomic region of Chinese Spring chromosome 3BS. The genetic and physical maps of Sb3 provide a framework for map-based cloning and marker-assisted selection (MAS) of the spot blotch resistance.
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Delin
authors.initials: D
authors.lastname: Li
authors.affiliation: China Rural Technology Development Center, Beijing, 100045, China.
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Li
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Qixin
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Chenggui
authors.initials: C
authors.lastname: Han
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China. zhiyongliu@cau.edu.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000472:pathogenicity*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D005799:Genes, Dominant; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D018895:Microsatellite Repeats; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D005819:Genetic Markers