title: Fabrication of pluronic and methylcellulose for etidronate delivery and their application for osteogenesis.
abstract: Novel hydrogels were prepared by blending 4% (w/w) methylcellulose (MC) with various concentrations of 12, 14, 16, 18 and 20% (w/w) pluronic F127 (PF) to form injectable implant drug delivery systems. The blends formed gels using lower concentrations of PF compared to when using PF alone. Etidronate sodium (EDS) at a concentration of 4×10(-3)M was loaded into these blends for producing an osteogenesis effect. The pure gels or EDS loaded gels exhibited cytocompatibility to both the osteoblast (MC3T3-E1) and myoblast (C2C12) cell lines whereas the gels of 16PF, 18PF and 20PF were very cytotoxic to the cells. The EDS loaded gels demonstrated significantly greater alkaline phosphatase (ALP) activities compared to the pure gels. The longer exposure time periods of the samples to the cells, the greater was the ALP activity. These EDS loaded gels significantly increased proliferation of both cell lines thus indicating a bone regeneration effect. The PF/MC blends prolonged the in vitro release of EDS for more than 28 days. Based on the in vitro degradation test, the MC extensively improved the gel strength of the PF and delayed the degradation of the gels thus making them more functional for a sustained drug delivery for osteogenesis.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90110, Thailand.
authors.forename: Aparna Sai Laxmi
authors.initials: ASL
authors.lastname: Rangabhatla
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90110, Thailand; PSU Center of Excellence for Drug Delivery System, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90110, Thailand. Electronic address: vimon.t@psu.ac.th.
authors.forename: Vimon
authors.initials: V
authors.lastname: Tantishaiyakul
authors.affiliation: Department of Pharmaceutical Techonolgy, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90110, Thailand.
authors.forename: Kwunchit
authors.initials: K
authors.lastname: Oungbho
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90110, Thailand.
authors.forename: Onpreeya
authors.initials: O
authors.lastname: Boonrat
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000494:pharmacology; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D065546:Drug Liberation; D012968:Etidronic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D020100:Hydrogels; D008747:Methylcellulose / Q000737:chemistry*; D051379:Mice; D032446:Myoblasts / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D020442:Poloxamer / Q000737:chemistry; D013997:Time Factors
chemical_list: D050071:Bone Density Conservation Agents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D020442:Poloxamer; D008747:Methylcellulose; D000469:Alkaline Phosphatase; D012968:Etidronic Acid
keywords: Alkaline phosphatase activity; C2C12; Etidronate sodium; MC3T3-E1; Methylcellulose; Pluronic F127