title: Functional state modelling approach validation for yeast and bacteria cultivations.
abstract: In this paper, the functional state modelling approach is validated for modelling of the cultivation of two different microorganisms: yeast (Saccharomyces cerevisiae) and bacteria (Escherichia coli). Based on the available experimental data for these fed-batch cultivation processes, three different functional states are distinguished, namely primary product synthesis state, mixed oxidative state and secondary product synthesis state. Parameter identification procedures for different local models are performed using genetic algorithms. The simulation results show high degree of adequacy of the models describing these functional states for bothS. cerevisiaeandE. colicultivations. Thus, the local models are validated for the cultivation of both microorganisms. This fact is a strong structure model verification of the functional state modelling theory not only for a set of yeast cultivations, but also for bacteria cultivation. As such, the obtained results demonstrate the efficiency and efficacy of the functional state modelling approach.
authors.affiliation: Bioinformatics and Mathematical Modelling, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Olympia
authors.initials: O
authors.lastname: Roeva
authors.affiliation: QSAR and Molecular Modelling, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Tania
authors.initials: T
authors.lastname: Pencheva
keywords: E. coli; S. cerevisiae; cultivation; functional state; modelling