title: Metabonomic study on the plasma of streptozotocin-induced diabetic rats treated with Ge Gen Qin Lian Decoction by ultra high performance liquid chromatography-mass spectrometry.
abstract: Changes in endogenous metabolites in the plasma of streptozotocin (STZ)-induced diabetic rats treated with Ge Gen Qin Lian Decoction (GGQLD) were studied. The endogenous compounds in plasma were detected using ultra high performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS). Rats were divided into three groups: control, model, and administration (4.95g crude drug/kg body weight). After the final administration, plasma samples from the three groups were analyzed using metabonomics. The three sample groups could be clearly distinguished. The administration group exhibited a distinct return to the levels of phytosphingosine and dihydrosphingosine of the control group according to the principal component analysis score, and the corresponding biomarkers were defined. Significant changes in endogenous metabolites, such as dihydrosphingosine, phytosphingosine, cholylglycine, and pantothenic acid, were identified in STZ-induced diabetic rats. These biochemical changes are associated with the metabolism of sphingolipids, fats, and acetyl coenzyme-A, which could be useful to further investigate the characteristics of STZ-induced diabetes mellitus and the therapeutic mechanism of action of GGQLD. This metabonomic analysis could provide a useful starting point toelucidate the therapeutic effects and mechanism of action of GGQLD in diabetes mellitus.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China; Research Center for Differentiation and Development of Basic Theory of TCM, University of Jiangxi TCM, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Research Center for Differentiation and Development of Basic Theory of TCM, University of Jiangxi TCM, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Research Center for Differentiation and Development of Basic Theory of TCM, University of Jiangxi TCM, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Research Center for Differentiation and Development of Basic Theory of TCM, University of Jiangxi TCM, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Bingtao
authors.initials: B
authors.lastname: Li
authors.affiliation: Research Center for Differentiation and Development of Basic Theory of TCM, University of Jiangxi TCM, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China. Electronic address: hshzhang@whu.edu.cn.
authors.forename: Huashan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D013311:Streptozocin
keywords: Diabetes; Ge Gen Qin Lian Decoction; Metabonomics; UHPLC-Q-TOF