title: The Synergic Anti-inflammatory Impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on Intestinal Epithelial Cells in a DSS-induced Colitis Model.
abstract: We investigated the synergic anti-inflammatory activity of Gleditsia sinensis Lam. (GS) extract and Lactobacillus brevis KY21 both in vitro and in vivo. Western blot analysis and immunostaining showed that AKT phosphorylation that increased by the exposure of LPS were significantly decreased by the presence of either GS extract or L. brevis KY21. In addition, p65 intracellular transport was critically inhibited by GS extract and L. brevis KY21. We further studied these effects using an in vivo dextran sulfate sodium (DSS)-induced mouse model. Body weight, food intake, and clinical scores were dramatically decreased after treatment with DSS, whereas these effects were palliated by the addition of GS extract and L. brevis KY21. Importantly, transcription of genes encoding pro-inflammatory cytokines including IL-1β, TNF-α, and IFN-γ in mesenteric lymph nodes (MLN) and the spleen were increased by DSS treatment, whereas they were inhibited by the presence of GS extract and L. brevis KY21.
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University, Jeonju 54896, Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Division of Food Bioscience and Technology, Korea University, Seoul 02841, Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Koh
authors.affiliation: Division of Food Bioscience and Technology, Korea University, Seoul 02841, Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Ahn
authors.affiliation: Division of Animal Science, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
authors.affiliation: Division of Food Bioscience and Technology, Korea University, Seoul 02841, Korea.
authors.forename: Sea Hun
authors.initials: SH
authors.lastname: Kim
keywords: DSS; anti-inflammatory activity; herbal extract; probiotic bacteria