title: In Vivo Iron-Chelating Activity and Phenolic Profiles of the Angel's Wings Mushroom, Pleurotus porrigens (Higher Basidiomycetes).
abstract: Pleurotus porrigens is an culinary-medicinal mushroom. It is locally called sadafi and is found in the northern regions of Iran, especially in Mazandaran. This mushroom is used to prepare a variety of local and specialty foods. Because of the phenol and flavonoid contents and the strong iron-chelating activity of this mushroom, it was selected for an assay of in vivo iron-chelating activity. Methanolic extract was administered intraperitoneally to iron-overloaded mice at two dosages (200 and 400 mg/kg/24 hours) for a total of 20 days, with a frequency of 5 times a week for 4 successive weeks. The total iron content was determined by atomic absorption spectroscopy. Plasma Fe3+ content was determined using a kit. Liver sections were stained by hematoxylin and eosin and Perls stain. A significant decrease in the plasma concentration of iron was observed in mice treated with extracts (P < 0.001). The animals showed a dramatic decrease in plasma Fe3+ content when compared with the control group (P < 0.001). Also, Perls stain improved the smaller amount of deposited iron in the liver of iron-overloaded mice treated with the extract. Liver sections revealed a marked reduction in the extent of necrotic hepatocytes, fibrous tissues, and pseudo-lobules. A high-performance liquid chromatography method was developed to simultaneously separate 7 phenolic acids in extract. Rutin (1.784 ± 0.052 mg g(-1) of extract) and p-coumaric acid (1.026 ± 0.043 mg g(-1) of extract) were detected as the main flavonoid and phenolic acids in extract, respectively. The extract exhibited satisfactory potency to chelate excessive iron in mice, potentially offering new natural alternatives to treat patients with iron overload. More studies are needed to determine which compounds are responsible for these biological activities.
authors.affiliation: Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran; Student Research Committee, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Khalili
authors.affiliation: Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Ebrahimzadeh
authors.affiliation: Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mehrnoush
authors.initials: M
authors.lastname: Kosaryan
mesh_terms: D000363:Agaricales; D000818:Animals; D001688:Biological Products / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology*; D004032:Diet; D005419:Flavonoids / Q000494:pharmacology*; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008815:Mice, Inbred Strains; D010636:Phenols / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry*; D011422:Propionates; D012431:Rutin / Q000494:pharmacology
chemical_list: D001688:Biological Products; D002614:Chelating Agents; D003373:Coumaric Acids; D005419:Flavonoids; D010636:Phenols; D011422:Propionates; D012431:Rutin; D007501:Iron; C495469:p-coumaric acid