title: Frequency of γδ T Cells and Invariant Natural Killer T Cells in Helicobacter Pylori-infected Patients with Peptic Ulcer and Gastric Cancer.
abstract: To clarify the effect of γδ T cells and invariant Natural Killer T (iNKT) cells in pathophysiology of dyspeptic disorders, number of these two cells in patients with non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), and gastric cancer (GC) were compared.Patients with dyspepsia were divided into three groups of NUD, PUD, and GC according to their endoscopic and histopathological examinations. Helicobacter pylori infection was diagnosed by rapid urease test and histopathology. The number of peripheral blood CD3+TCRγδ(+) T cells and CD3+Va24Ja18+ iNKT cells were determined by flow cytometry. Immunohistochemistry (IHC) was also used for identifying the TCRγδ+ cells.Forty two patients with NUD (31.6%), 44 with PUD (33.1%), and 47 with GC (35.3%) were included in the study. The frequency of CD3+TCRγδ(+) T cells in peripheral blood of patients with GC (2.71±0.25) was significantly lower than that in NUD (3.97±0.32, p<0.05) and PUD groups (3.87±0.32, p<0.05). However, there was no significant difference in CD3+TCRγδ(+) T cell percentage between the NUD and PUD groups. The frequency of TCRγδ(+) lymphocytes was significantly lower in tissue samples from patients with GC (4.81±0.53) than in NUD (11.09±1.09, p<0.0001) and PUD groups (11.11±1.01, p<0.0001). Also, we could not find any significant difference in the percentage of mucosal TCRγδ+ cells between the NUD and PUD groups. The results showed no significant difference in iNKT cells percentage among the three groups of patients.The results suggest that decreasing number of γδ T cells may be related to development and progression of gastric cancer.
authors.affiliation: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shadman
authors.affiliation: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Rajabian
authors.affiliation: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran AND Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Ajami
authors.affiliation: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Hussein-Nattaj
authors.affiliation: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran AND Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Rafiei
authors.affiliation: Inflammatory Diseases of Upper GI Tract Research Center, Imam Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Hosseini
authors.affiliation: Inflammatory Diseases of Upper GI Tract Research Center, Imam Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Tarang
authors.initials: T
authors.lastname: Taghvaei
authors.affiliation: Department of pathology, Islamic Azad University, Sari Branch, Sari, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Abbasi
authors.affiliation: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran AND Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Tehrani
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D001706:Biopsy; D004415:Dyspepsia / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016145:Endoscopy, Digestive System; D005260:Female; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000378:metabolism; D016481:Helicobacter Infections / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D055611:Natural Killer T-Cells / Q000276:immunology*; D010437:Peptic Ulcer / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology / Q000378:metabolism*; D013270:Stomach / Q000276:immunology / Q000473:pathology; D013274:Stomach Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016176:T-Lymphocyte Subsets
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: Antigen; Helicobacter pylori; Invariant Natural Killer T Cells; Peptic Ulcer; Receptor; Stomach Neoplasms; T-Cell, gamma-delta