title: Immunomodulatory effect of mesenchymal stem cells on the immune response of macrophages stimulated by Aspergillus fumigatus conidia.
abstract: Mesenchymal stem cells (MSCs) are known to exert potent immunosuppression and anti-inflammatory effects. There is growing interest in their use for immunotherapy for controlling inflammation as well as acute organ injury. However, there are few reports regarding MSC's immunomodulatory effects in the settings of fungal infection. In this study, we attempted to examine the immunomodulatory effects of MSCs in response to Aspergillus fumigatus We measured the cytokine response of murine MSCs on the immune response of murine macrophages (J774A.1 cells) evoked by A. fumigatus conidia. In addition, we evaluated the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the MSC-related cytokine response and fungal growth. As a results, after conidia stimulation, tumor necrosis factor (TNF)-α was down-regulated and interleukin (IL)-10 was up-regulated in MSC-treated J774A.1 cells when compared to J774A.1 cells alone. In addition, fungal growth was reduced in MSC-treated J774A.1 cells when compared to J774A.1 cells, which recovered by GM-CSF. However, the effect of MSCs on the cytokine response was not reversed by GM-CSF. NF-κB translocation decreased in MSC-treated J774A.1 cells compared to J774A.1 cells alone. In conclusion, MSCs demonstrate immunomodulatory properties in both aspects of cytokines and fungal growth. The anti-inflammatory effect of MSCs with regard to cytokine response might be associated with decreased NF-κB translocation, and is not reversed by GM-CSF.
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Sung-Yeon
authors.initials: SY
authors.lastname: Cho
authors.affiliation: Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kwon
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea Institute of Clinical Medicine Research, Yeouido St. Mary's Hospital, Seoul, Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Choi
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Dong-Gun
authors.initials: DG
authors.lastname: Lee
authors.affiliation: Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Chulmin
authors.initials: C
authors.lastname: Park
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Park
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea.
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Yoo
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Vaccine Bio Research Institute, The Catholic University of Korea, Seoul, Korea cmcjh@catholic.ac.kr.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000818:Animals; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D002460:Cell Line; D018920:Coculture Techniques; D016207:Cytokines / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D013172:Spores, Fungal / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: Aspergillus fumigatus; Cytokines; Immunomodulation; Macrophages; Mesenchymal Stromal Cells