title: Dipeptidyl Peptidase IV-Inhibitory Peptides Derived from Silver Carp (Hypophthalmichthys molitrix Val.) Proteins.
abstract: The dipeptidyl peptidase IV (DPP-IV)-inhibitory bioactivity of silver carp protein (SCP) hydrolysates were investigated, and their containing efficacious DPP-IV-inhibitory peptides were explored by in silico hydrolysis analysis, peptide separation combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification, and chemical synthesis. SCP hydrolysates generated by six proteases all showed efficient DPP-IV-inhibitory activities, and Neutrase-generated hydrolysates had the greatest DPP-IV inhibition (IC50 of 1.12 mg/mL). In silico Neutrase hydrolysis revealed hundreds of fragments released from myosin, actin, and collagen of SCPs, which include different Pro-motif peptides but only three reported peptidic DPP-IV inhibitors with moderate or weak bioactivity. In addition, three new DPP-IV-inhibitory peptides were identified using LC-MS/MS; in particular, LPIIDI and APGPAGP showed high DPP-IV-inhibitory activity with IC50 of 105.44 and 229.14 μM, respectively, and behaved in competitive/non-competitive mixed-type DPP-IV inhibition mode. The results indicate that the SCP-derived DPP-IV-inhibitory peptides could be potential functional ingredients in the diabetic diet.
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Chen
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.
authors.forename: Xiling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University , Beijing 100193, People's Republic of China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.|Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, People's Republic of China.
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry*; D029941:Fish Proteins / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D029941:Fish Proteins; D010455:Peptides; D010447:Peptide Hydrolases; D018819:Dipeptidyl Peptidase 4
keywords: LC−MS/MS identification; Pro-motif peptide; dipeptidyl peptidase IV; in silico analysis; peptide inhibitor; silver carp protein