title: Epithelial sodium channel (ENaC) family: Phylogeny, structure-function, tissue distribution, and associated inherited diseases.
abstract: The epithelial sodium channel (ENaC) is composed of three homologous subunits and allows the flow of Na(+) ions across high resistance epithelia, maintaining body salt and water homeostasis. ENaC dependent reabsorption of Na(+) in the kidney tubules regulates extracellular fluid (ECF) volume and blood pressure by modulating osmolarity. In multi-ciliated cells, ENaC is located in cilia and plays an essential role in the regulation of epithelial surface liquid volume necessary for cilial transport of mucus and gametes in the respiratory and reproductive tracts respectively. The subunits that form ENaC (named as alpha, beta, gamma and delta, encoded by genes SCNN1A, SCNN1B, SCNN1G, and SCNN1D) are members of the ENaC/Degenerin superfamily. The earliest appearance of ENaC orthologs is in the genomes of the most ancient vertebrate taxon, Cyclostomata (jawless vertebrates) including lampreys, followed by earliest representatives of Gnathostomata (jawed vertebrates) including cartilaginous sharks. Among Euteleostomi (bony vertebrates), Actinopterygii (ray finned-fishes) branch has lost ENaC genes. Yet, most animals in the Sarcopterygii (lobe-finned fish) branch including Tetrapoda, amphibians and amniotes (lizards, crocodiles, birds, and mammals), have four ENaC paralogs. We compared the sequences of ENaC orthologs from 20 species and established criteria for the identification of ENaC orthologs and paralogs, and their distinction from other members of the ENaC/Degenerin superfamily, especially ASIC family. Differences between ENaCs and ASICs are summarized in view of their physiological functions and tissue distributions. Structural motifs that are conserved throughout vertebrate ENaCs are highlighted. We also present a comparative overview of the genotype-phenotype relationships in inherited diseases associated with ENaC mutations, including multisystem pseudohypoaldosteronism (PHA1B), Liddle syndrome, cystic fibrosis-like disease and essential hypertension.
authors.affiliation: Laboratory of Cell Biology, Faculty of Natural Sciences, Ariel University, Ariel, Israel. Electronic address: mbiochem@gmail.com.
authors.forename: Israel
authors.initials: I
authors.lastname: Hanukoglu
authors.affiliation: Division of Pediatric Endocrinology, E. Wolfson Medical Center, Holon, Israel; Sackler School of Medicine, Tel-Aviv University, Tel Aviv, Israel.
authors.forename: Aaron
authors.initials: A
authors.lastname: Hanukoglu
mesh_terms: D000818:Animals; D003550:Cystic Fibrosis / Q000235:genetics* / Q000473:pathology; D053503:Epithelial Sodium Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000075222:Essential Hypertension; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D009928:Organ Specificity; D010802:Phylogeny; D011487:Protein Conformation; D012964:Sodium / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D053503:Epithelial Sodium Channels; D012964:Sodium
keywords: Epithelia; Evolution; Ion channels; Kidney; Renin–angiotensin–aldosterone system; Transmembrane proteins