title: Effect of Porcine Placenta Extract from Subcritical Water Extraction on Photodamage in Human Keratinocytes.
abstract: The objective of this study was to evaluated the photoprotective effects of porcine placenta extract (PPE) on ultraviolet B (UVB)-induced oxidative stress in human keratinocytes (HaCaT) to evaluate its functional activities as a skin food ingredient. PPE prepared by subcritical water extraction was termed SPE, and subsequently digested by enzymes to prepare E-SPE. Increased intracellular reactive oxygen species (ROS) levels (192.0%) induced by UVB were decreased by SPE and E-SPE. SPE had more effective ROS scavenging activity than E-SPE treatment. UVB treatment increased expression of tissue inhibitor of metalloproteinase 1 (TIMP-1), and this elevated expression was decreased by E-SPE treatment. High-dose treatment with E-SPE (50 and 100 µg/mL) reduced TIMP-1 expression levels of UVB-C (control) to 33.5 and 34.6%, respectively. In contrast, at low SPE doses (1 and 10 µg/mL), the treatment slightly decreased TIMP-1 expression levels to 73.3% and 71.3% of UVB-C, respectively. In conclusion, the present study demonstrated the protective effect of SPE and E-SPE against UVB damage in keratinocytes via ROS scavenging, down-regulating MMP-2 expression and up-regulating TIMP-1 expression. This highlights the potential for SPE as an ingredient in the preparation of functional food against photoaging.
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.affiliation: BK Bio Co. Ltd. Sungnam 462-819, Korea.
authors.forename: Bok Kyung
authors.initials: BK
authors.lastname: Han
authors.affiliation: Department of Food Technology, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Beauty and Art, Suwon Women's University, Suwon 441-748, Korea.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Hong
authors.affiliation: Department of Home Economic Education, Jeonju University, Jeonju 560-759, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jung
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
keywords: MMP-2; ROS; TIMP-1; UVB; porcine placenta extract; subcritical water extraction