title: High frequency of Candida fabianii among clinical isolates biochemically identified as Candida pelliculosa and Candida utilis.
abstract: Clinical yeast isolates belonging to Candida pelliculosa, Candida utilis and Candida fabianii are difficult to distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis and C. fabianii in clinical samples and to compare their minimum inhibitory concentrations (MICs) to systemic antifungals. Two hundred and forty-eight clinical yeast isolates obtained from eight large hospitals in the Czech Republic were included in this study. Identification was performed biochemically using ID 32C kit and by MALDI-TOF MS. MICs were determined using colorimetric broth dilution Sensititre YeastOne panels. From a total number of 248 isolates, 175 were identified as C. pelliculosa and 73 as C. utilis using the biochemical kit. In contrast, MALDI-TOF MS identified 222 isolates as C. fabianii, 20 as C. pelliculosa and 6 as C. utilis. The highest mean MICs were found in C. fabianii and, regardless of the studied species, in isolates from blood cultures and central venous catheters. MALDI-TOF MS revealed C. fabianii to be most prevalent in clinical samples as compared with the other studied species. Higher MIC values in C. fabianii support the importance of correct identification of this species.
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Svobodova
authors.affiliation: Research & Development Department, GeneProof a.s., Brno, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Bednarova
authors.affiliation: Department of Microbiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Ruzicka
authors.affiliation: Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Chrenkova
authors.affiliation: Laboratory of Clinical Mycology, Public Health Institute Ostrava, Ostrava, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Dobias
authors.affiliation: Laboratory of Parasitology and Mycology, Ceske Budejovice Hospital, Ceske Budejovice, Czech Republic.
authors.forename: N
authors.initials: N
authors.lastname: Mallatova
authors.affiliation: Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, Hradec Kralove, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Buchta
authors.affiliation: Department of Clinical Microbiology, University Hospital Brno, Brno, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Kocmanova
authors.affiliation: Mycology Laboratory, Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics of the General University Hospital and of the First Faculty of Medicine of Charles University in Prague, Prague, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Olisarova
authors.affiliation: State Veterinary Institute Olomouc, Olomouc, Czech Republic.
authors.forename: N
authors.initials: N
authors.lastname: Stromerova
authors.affiliation: Cellular and Molecular Immunology Research Unit (CMIRU), Department of Medical Technology, Faculty of Allied Health Science, Naresuan University, Phitsanulok, Thailand.
authors.forename: Y
authors.initials: Y
authors.lastname: Thongsri
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Hamal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification* / Q000302:isolation & purification*; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D006761:Hospitals; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016533:Mycological Typing Techniques; D015995:Prevalence; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D000935:Antifungal Agents
keywords: 
  Candida fabianii
  ; MALDI-TOF MS; antifungal susceptibility testing; biochemical identification