title: The Effect of Stromal Integrin β3-Deficiency on Two Different Tumors in Mice.
abstract: There is an increasing focus on the tumor microenvironment in carcinogenesis. Integrins are important receptors and adhesion molecules in this environment and have been shown to be involved in cell adhesion, proliferation, differentiation and migration. The present study aimed to evaluate the effect of stromal integrin β3-deficiency on tumor growth, angiogenesis, interstitial fluid pressure (PIF), fibrosis and metastasis in a murine breast cancer (4T1) and a prostate tumor (RM11) model. We showed that stromal integrin β3-deficiency led to an elevation in PIF that correlated to a shift towards thicker collagen fibrils in the 4T1 mammary tumor. In the RM11 prostate carcinoma model there was no effect of integrin β3-deficiency on PIF and collagen fibril thickness. These findings support the notion that changes in the collagen scaffold influence PIF, and also indicate that there must be important crosstalk between the stroma and tumor cells, in a tumor cell line specific manner. Furthermore, stromal integrin β3-deficiency had no effect on tumor growth or angiogenesis in both tumor models and no effect on lung metastasis in the 4T1 mammary tumor model. In conclusion, the stromal β3 integrin influence PIF, possibly via its effect on the structure of the collagen network, in a tumor cell line dependent manner.
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. inga.reigstad@uib.no.|Matrix Biology Group, Haukeland University Hospital, N-5021 Bergen, Norway. inga.reigstad@uib.no.
authors.forename: Inga
authors.initials: I
authors.lastname: Reigstad
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. kristina.sortland@uib.no.|Matrix Biology Group, Haukeland University Hospital, N-5021 Bergen, Norway. kristina.sortland@uib.no.
authors.forename: Kristina
authors.initials: K
authors.lastname: Sortland
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. trude.skogstrand@uib.no.|Matrix Biology Group, Haukeland University Hospital, N-5021 Bergen, Norway. trude.skogstrand@uib.no.
authors.forename: Trude
authors.initials: T
authors.lastname: Skogstrand
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. linda.stuhr@uib.no.|Center of Cancer Biomarkers, University of Bergen, N-5020 Bergen, Norway. linda.stuhr@uib.no.
authors.forename: Rolf K
authors.initials: RK
authors.lastname: Reed
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. rolf.reed@uib.no.|Center of Cancer Biomarkers, University of Bergen, N-5020 Bergen, Norway. rolf.reed@uib.no.
authors.forename: Linda
authors.initials: L
authors.lastname: Stuhr
keywords: angiogenesis; fibrosis; interstitial fluid pressure; metastasis; tumor growth