title: Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route.
abstract: The lack of an immunocompetent animal model for dengue mimicking the disease in humans is a limitation for advances in this field. Inoculation by intracerebral route of neuroadapted dengue strains in mice is normally lethal and provides a straightforward readout parameter for vaccine testing. However, systemic effects of infection and the immune response elicited in this model remain poorly described. In the present work, BALB/c mice infected by the intracerebral route with neuroadapted DENV2 exhibited several evidences of systemic involvement. DENV-inoculated mice presented virus infective particles in the brain followed by viremia, especially in late stages of infection. Infection induced cellular and humoral responses, with presence of activated T cells in spleen and blood, lymphocyte infiltration and tissue damages in brain and liver, and an increase in serum levels of some pro-inflammatory cytokines. Data highlighted an interplay between the central nervous system commitment and peripheral effects under this experimental condition.
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: E R A
authors.initials: ER
authors.lastname: Oliveira
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: J F S
authors.initials: JF
authors.lastname: Amorim
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: M V
authors.initials: MV
authors.lastname: Paes
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Azevedo
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: A J S
authors.initials: AJ
authors.lastname: Gonçalves
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: S M
authors.initials: SM
authors.lastname: Costa
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Mantuano-Barradas
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: T F
authors.initials: TF
authors.lastname: Póvoa
authors.affiliation: Laboratory on Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: de Meis
authors.affiliation: Pathological Anatomy, Hospital Gaffrée Guinle, Federal University from the State of Rio de Janeiro (UNIRIO), RJ, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Basílio-de-Oliveira
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: A C M A
authors.initials: AC
authors.lastname: Nogueira
authors.affiliation: Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. Electronic address: ada@ioc.fiocruz.br.
authors.forename: A M B
authors.initials: AM
authors.lastname: Alves
mesh_terms: D000818:Animals; D054022:Cerebrum / Q000473:pathology / Q000821:virology*; D003715:Dengue / Q000473:pathology / Q000821:virology*; D003716:Dengue Virus / Q000472:pathogenicity / Q000502:physiology*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014774:Virulence
keywords: Central nervous system; Dengue; Immune response; Intracerebral infection; Mouse model