title: Production of biobutanol from cellulose hydrolysate by the Escherichia coli co-culture system.
abstract: The commercialization of the n-butanol bioprocess is largely dependent on the price of feedstocks. Renewable cellulose appears to be an appealing feedstock. The microbial production of n-butanol still remains challenging because of the limited availability of intracellular NADH. To address this issue, an Escherichia coli strain carrying the clostridial CoA-dependent pathway was supplied with heterologous formate dehydrogenase. With the cellulose hydrolysate of rice straw, this single strain produced cellulosic biobutanol with a production yield at 35% of the theoretical and a productivity of 0.093 g L(-1) h(-1). In an alternative method, the production involved a co-culture system consisting of two separate strains provided with the full CoA-dependent pathway. This system achieved a production yield and productivity reaching 62.8% of the theoretical and 0.163 g L(-1) h(-1), respectively. The result indicates that the E. coli co-culture system is technically promising for the production of cellulosic biobutanol.
authors.affiliation: Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung 40724, Taiwan.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Saini
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
authors.forename: Chung-Jen
authors.initials: CJ
authors.lastname: Chiang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung 40724, Taiwan Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan ypchao@fcu.edu.tw.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Chao
mesh_terms: D020001:1-Butanol / Q000032:analysis / Q000378:metabolism*; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology / Q000235:genetics; D018920:Coculture Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005560:Formate Dehydrogenases / Q000378:metabolism; D006868:Hydrolysis; D009243:NAD / Q000378:metabolism; D012275:Oryza / Q000378:metabolism
chemical_list: D056804:Biofuels; D009243:NAD; D020001:1-Butanol; D002482:Cellulose; D005560:Formate Dehydrogenases
keywords: E. coli co-culture; cellulose hydrolysate; n-butanol; rice straw