title: Correlation analysis of urine metabolites and clinical staging in patients with ovarian cancer.
abstract: This study is to investigate the correlation between urine metabolites and clinical staging in patients with ovarian cancer. The urina sanguinis from 56 cases of primary epithelial ovarian cancer patients and 15 healthy volunteers was collected and the urine metabolites were extracted. Ultra high performance liquid chromatography/time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis was performed. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to analyze the mass spectrometry data. Database retrieval and comparison of the screened metabolites were performed and one-way ANOVA and least significant difference (LSD) t test were carried out. PCA analysis of UPLC-Q-TOF-MS results showed that the score plots of samples from healthy people and patients with ovarian cancer at different clinical stages were separated. Further PLS-DA analysis significantly improved the classification results. The R(2)X was 0.757, the R(2)Y was 0.977 and the Q(2)Y was 0.87, indicating that the model stability and predictability were good. Eight metabolites, including N-acetylneuraminic acid-9-phosphate, 5'-methioadenosine, uric acid-3-nucleoside, pseudouridine, L-valine, succinic acid, L-proline and β-nicotinamide mononucleotide were identified. The contents of these metabolites increased with the development of the disease. There was correlation between urine metabolites and clinical staging in patients with ovarian cancer.
authors.affiliation: The Food and Drug Testing Room of Analysis and Testing Center of Shandong Province Jinan 250014, P. R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: The Food and Drug Testing Room of Analysis and Testing Center of Shandong Province Jinan 250014, P. R. China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynecology, Central Hospital of Jinan 250013 P. R. China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Yin
authors.affiliation: The Food and Drug Testing Room of Analysis and Testing Center of Shandong Province Jinan 250014, P. R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: The Food and Drug Testing Room of Analysis and Testing Center of Shandong Province Jinan 250014, P. R. China.
authors.forename: Qinglei
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University Jinan 250012, P. R. China.
authors.forename: Peihai
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Shandong University Jinan 250012, P. R. China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Bi
keywords: Ovarian cancer; metabonomics; staging; ultra high performance liquid chromatography/time-of-flight mass spectrometry; urine