title: Living Single Cell Analysis Platform Utilizing Microchannel, Single Cell Chamber, and Extended-nano Channel.
abstract: Single cell analysis has been of great interest in recent years. In particular, to achieve living single cell analysis is the ultimate goal to study the dynamic process of the single cell. However, single cell volume is pL in scale, and it is difficult to realize living single cell analysis, even by microfluidic technology (nL-sub nL). Herein, a novel microfluidic platform was developed by integrating a single cell chamber and an extended-nano channel (aL-fL volume). A single cell was isolated and cultured for more than 12 h by pressure-driven flow control. In addition, an electric resistance measurement method was developed to monitor the cell viability without fluorescence labeling. This platform will provide a new method for living single cell analysis by utilizing the novel analytical functions of the extended-nano space.
authors.affiliation: Department of Bioengineering, School of Engineering, The University of Tokyo.
authors.forename: Ling
authors.initials: L
authors.lastname: Lin
authors.forename: Kazuma
authors.initials: K
authors.lastname: Mawatari
authors.forename: Kyojiro
authors.initials: K
authors.lastname: Morikawa
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D017097:Electric Impedance; D042783:Endothelial Cells / Q000166:cytology; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*