title: Elevation of HLA-G-expressing DC-10 cells in patients with gastric cancer.
abstract: DC-10 is a distinct subset of human tolerogenic dendritic cells (DCs) which express high levels of human leukocyte antigen-G (HLA-G). DC-10 could induce adaptive type 1 regulatory T cells through the IL-10 dependent ILT4/HLA-G signaling pathway. However, the significance of DC-10 in malignancies remains unclear. In this study, the frequency and mean fluorescence intensity (MFI) of HLA-G+ DC-10 in the peripheral blood of 124 patients with gastric cancer (GC) and 130 normal controls was analyzed with flow cytometry. Plasma sHLA-G was analyzed with ELISA. Results showed both the percentages of peripheral HLA-G+ DC-10 (median: 0.13% vs 0.01%; p<0.01) and MFI of HLA-G on these cells (median: 310.0 vs 91.5; p<0.01) were dramatically increased in GC patients than in normal controls. The frequency of HLA-G+ DC-10 in GC patients was strongly relative to the tumor grade (p=0.021). sHLA-G levels in GC patients were significantly higher than in healthy controls (median: 85.80U/ml vs 61.20U/ml; p<0.01). There was no significant correlation between the percentage of DC-10 and plasma sHLA-G (p>0.05). However, the increased HLA-G+ DC-10, HLA-G MFI and plasma sHLA-G in patients with gastric cancer could be a diagnostic factor with the area under the ROC curve with 0.947 (p<0.01), 0.882 (p<0.01) and 0.700 (p<0.01) respectively. Given the immune tolerant function of DC-10 could play, the increased DC-10 might play an important role in immune suppression for patients with gastric cancer, while more studies are necessary to illustrate the clinical relevance of DC-10 in cancer patients.
authors.affiliation: Medical Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Xu
authors.affiliation: Medical Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Wei-Wu
authors.initials: WW
authors.lastname: Shi
authors.affiliation: Health Care Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Tong-Tong
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: Medical Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Lv
authors.affiliation: Medical Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Li
authors.affiliation: Human Tissue Bank, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Aifen
authors.initials: A
authors.lastname: Lin
authors.affiliation: Medical Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, Zhejiang, China. Electronic address: yanwhcom@yahoo.com.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Yan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001798:Blood Proteins / Q000378:metabolism*; D002452:Cell Count; D002469:Cell Separation; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D059951:HLA-G Antigens / Q000378:metabolism*; D006801:Humans; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D001798:Blood Proteins; D059951:HLA-G Antigens; C508609:IL10 protein, human; D016753:Interleukin-10
keywords: DC-10; Gastric cancer; HLA-G