title: Real-time monitoring of the metabolic capacity of ex vivo rat olfactory mucosa by proton transfer reaction mass spectrometry (PTR-MS).
abstract: Olfactory mucosa (OM) can metabolise odorant volatile organic compounds through various enzymatic mechanisms to produce odorous or non-odorous metabolites. Preliminary ex vivo studies using headspace-gas chromatography (HS-GC) revealed the formation of metabolites when odorant molecules were injected in the headspace above a fresh explant of rat olfactory mucosa. However, this method did not allow accessing the data during the first 5 min of contact between the odorant and the mucosa; thus limiting the olfactory biological significance. Using a direct-injection mass spectrometry technique with a proton transfer reaction instrument (PTR-MS), we have been able, for the first time, to investigate the first moments of the enzymatic process of the metabolic capacity of ex vivo rat olfactory mucosa in real time. Using ethyl acetate as a model volatile odorous substrate, we demonstrated here for the first time that this odorant could be metabolised by an ex vivo olfactory mucosa within seconds, producing ethanol as metabolite.
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.|INRA, UMR1324 CSGA, F-21000, Dijon, France.|Université de Bourgogne, UMR CSGA, F-21000, Dijon, France.
authors.forename: Rachel
authors.initials: R
authors.lastname: Schoumacker
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.|INRA, UMR1324 CSGA, F-21000, Dijon, France.|Université de Bourgogne, UMR CSGA, F-21000, Dijon, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Robert-Hazotte
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.|INRA, UMR1324 CSGA, F-21000, Dijon, France.|Université de Bourgogne, UMR CSGA, F-21000, Dijon, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Heydel
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France.|INRA, UMR1324 CSGA, F-21000, Dijon, France.|Université de Bourgogne, UMR CSGA, F-21000, Dijon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Faure
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation (CSGA), F-21000, Dijon, France. jllequere@dijon.inra.fr.|INRA, UMR1324 CSGA, F-21000, Dijon, France. jllequere@dijon.inra.fr.|Université de Bourgogne, UMR CSGA, F-21000, Dijon, France. jllequere@dijon.inra.fr.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Le Quéré
mesh_terms: D000085:Acetates / Q000032:analysis / Q000378:metabolism; D000818:Animals; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008991:Monitoring, Physiologic / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D009831:Olfactory Mucosa / Q000378:metabolism*; D009924:Organ Culture Techniques; D011522:Protons; D017208:Rats, Wistar; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D000085:Acetates; D011522:Protons; D055549:Volatile Organic Compounds; C007650:ethyl acetate
keywords: Ethyl acetate; Metabolite; Olfaction; PTR-MS; Rat; XMEs