title: Enzymatic dehairing of goat skins using alkaline protease from Bacillus sp. SB12.
abstract: The present paper reports the production, purification and biochemical characterization of an extracellular alkaline protease from Bacillus sp. SB12. The enzyme has been used as an alternative to conventional chemicals treatment for dehairing of goat skins. The protease was optimally active at 37 °C and pH 9. Starch at 2% (w/v) was used as the best carbon source and the addition of yeast extract and peptone at 1% each supported the maximum level of protease production in the presence of 5 mM Ca(2+). Protease purification was performed with ammonium sulphate precipitation at 70% saturated fraction followed by dialysis and gel filtration chromatography using Sephadex G-100. The purified enzyme was homogeneous on non-denaturing PAGE and appeared as a single band with an apparent molecular weight of 41 kDa. This enzyme was moderately thermostable and has a wide pH stability range extending from pH 7 to 11. It showed high tolerance toward surfactants agents and organic solvents while it was completely inhibited by PMSF indicating the serine protease type. Purified protease was used to remove hair from goat skin proving its potential application in leather processing industry. The results revealed that the protease has enhanced the quality and physico-chemical properties of the skins while reducing the pollution.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Biology, Faculty of Sciences of Bizerte, University of Carthage, Tunisia; Laboratory of Microbial Ecology and Technology, Department of Biological and Chemical Engineering, National Institute of Applied Sciences and Technology, University of Carthage, Tunisia. Electronic address: brikisel@gmail.com.
authors.forename: Selmen
authors.initials: S
authors.lastname: Briki
authors.affiliation: Laboratory of Microbial Ecology and Technology, Department of Biological and Chemical Engineering, National Institute of Applied Sciences and Technology, University of Carthage, Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: Hamdi
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Biology, Faculty of Sciences of Bizerte, University of Carthage, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Landoulsi
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010450:Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004795:Enzyme Stability; D006041:Goats; D006197:Hair / Q000187:drug effects; D012867:Skin / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D010450:Endopeptidases; C087822:alkaline protease
keywords: Bacillus sp. SB12; Dehairing; Goat skins; Protease