title: Characterization of IgA1 protease as a surface protective antigen of Streptococcus suis serotype 2.
abstract: IgA1 protease of Streptococcus suis serotype 2 (SS2) has been proven to be relative with virulence and immunogenicity, however, its protective efficacy remained to be evaluated. The present study found evidence that immunization with purified recombinant IgA1 protease (600-1926aa) could induce high IgG antibody titers and could confer complete protection against a challenge with a lethal dose of SS2 in a mouse model. In addition, our findings confirmed that the IgA1 protease distributes on the surface of SS2. Therefore, the present study identified the virulence-associated protein, IgA1 protease, as a novel surface protective antigen of SS2.
authors.affiliation: National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, Hubei, 430070, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Xie
authors.affiliation: National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, Hubei, 430070, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, Hubei, 430070, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
authors.affiliation: National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China. Electronic address: andye8019@mail.hzau.edu.cn.
authors.forename: Anding
authors.initials: A
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D004195:Disease Models, Animal; D008565:Membrane Proteins / Q000276:immunology; D008807:Mice, Inbred BALB C; D012697:Serine Endopeptidases / Q000276:immunology*; D065288:Serogroup; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017011:Streptococcus suis / Q000145:classification / Q000201:enzymology* / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008565:Membrane Proteins; D022541:Streptococcal Vaccines; D014614:Vaccines, Synthetic; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase
keywords: IgA1 protease; Protective antigen; Streptococcus suis