title: Electrophoretic separation method for membrane pore-forming proteins in multilayer lipid membranes.
abstract: In this paper, we report on a novel electrophoretic separation and analysis method for membrane pore-forming proteins in multilayer lipid membranes (MLMs) in order to overcome the problems related to current separation and analysis methods of membrane proteins, and to obtain a high-performance separation method on the basis of specific properties of the lipid membranes. We constructed MLMs, and subsequently characterized membrane pore-forming protein behavior in MLMs. Through the use of these MLMs, we were able to successfully separate and analyze membrane pore-forming proteins in MLMs. To the best of our knowledge, this research is the first example of membrane pore-forming protein separation in lipid membranes. Our method can be expected to be applied for the separation and analysis of other membrane proteins including intrinsic membrane proteins and to result in high-performance by utilizing the specific properties of lipid membranes.
authors.affiliation: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsujimoto
authors.affiliation: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Umakoshi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D008051:Lipid Bilayers / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D008555:Melitten / Q000737:chemistry / Q000378:metabolism; D013301:Streptolysins / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008051:Lipid Bilayers; D013301:Streptolysins; C025964:streptolysin O; D008555:Melitten
keywords: Electrophoresis; Membrane pore-forming protein; Multilayer lipid membrane; Separation