title: Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.
abstract: During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to assemble a focused midbody structure and did not establish tight linkage to the plasma membrane, resulting in furrow regression. Thus augmin-dependent acentrosomal MTs and centrosomal MTs contribute to nonredundant targeting mechanisms of different cytokinesis factors, which are required for the formation of a functional contractile ring and midbody.
authors.affiliation: Creative Research Institution, Hokkaido University, Sapporo 001-0021, Japan Department of Life Sciences, School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan ruehara@sci.hokudai.ac.jp.
authors.forename: Ryota
authors.initials: R
authors.lastname: Uehara
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kamasaki
authors.affiliation: Creative Research Institution, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Hiruma
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Poser
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Kinya
authors.initials: K
authors.lastname: Yoda
authors.affiliation: Department of Life Sciences, School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Yajima
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter Campus, 1030 Vienna, Austria.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Gohta
authors.initials: G
authors.lastname: Goshima
mesh_terms: D000705:Anaphase / Q000502:physiology*; D000814:Aniline Compounds / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000502:physiology* / Q000648:ultrastructure; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; C000612281:augmin protein, human; C066737:RHOA protein, human; D020742:rhoA GTP-Binding Protein; C023650:aniline