title: Multilocus Sequence Types and Virulence Determinants of Hypermucoviscosity-Positive Klebsiella pneumoniae Isolated from Community-Acquired Infection Cases in Harbin, North China.
abstract: We investigated the molecular epidemiologic characteristics and virulence of hypermucoviscosity-positive Klebsiella pneumoniae in mainland China. We detected 16 hypermucoviscosity-positive strains in 65 total clinical isolates (24.62%). We found that 68.75% (11/16) of the positive strains had K2 genotype and carried the rmpA and iucA genes. Multilocus sequence typing revealed 5 sequence types (STs): ST65 [7], ST23 [4], ST86 [3], ST412 [1], ST375 [1], whereas the remaining 4 isolates were defined as other STs. The order of the median lethal dose values for the ST types was ST23 (2.19 × 10(3) CFU/mouse) < ST86 (1.70 × 10(4) CFU/mouse) < ST65 (5.05 × 10(7) CFU/mouse) < the other STs (1.90 × 10(8) CFU/mouse). In conclusion, the K2 with ST65 carrying rmpA and iucA was the most predominant among the hypermucoviscosity-positive K. pneumoniae strains obtained from community-acquired infection cases in Harbin, North China. Sequence types are a valuable tool to predict the risk of K. pneumoniae infection.
authors.affiliation: Department of Microbiology, Harbin Medical University.
authors.forename: Jizi
authors.initials: J
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qiu
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Chen
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.forename: James Opiyo
authors.initials: JO
authors.lastname: Ogutu
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D058885:Multilocus Sequence Typing; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D016019:Survival Analysis; D014774:Virulence; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D011135:Polysaccharides, Bacterial; D037521:Virulence Factors