title: Levels of Schistosoma mansoni Circulating Antigen in Chronic Hepatitis C Patients with Different Stages of Liver Fibrosis.
abstract: The goal of this study was to determine the levels of S. mansoni antigen in different liver fibrosis stages with chronic hepatitis C (CHC) Egyptian patients. A total of 174 CHC patients showing HCV-NS4 antigen and HCV- RNA in their sera were included. S. mansoni antigen was detected in serum using Western blot and ELISA. The levels of interferon-γ (IFN- γ) were determined using ELISA. The 50 kDa S. mansoni antigen discriminated patients infected with S. mansoni from healthy individuals with 0.93 area under curve (AUC), 92% sensitivity, and 97% specificity. The level of S. mansoni antigen (μg/ml) was significantly (P < 0.0001) increased with the progression of liver fibrosis stages (26.9 ± 17.5 in F1, 42.1 ± 25.2 in F2, 49.8 ± 30.3 in F3 and 62.2 ± 26.3 μg/mL in F4 liver cirrhosis), 26.9 ± 17.59 in significant fibrosis (F2-F4); 51.2 ± 27.9 in advanced fibrosis (F3-F4). A significant correlation (r = 0.506; P < 0.0001) was shown between the levels of the S. mansoni antigen and the HCV-NS4 antigen. In conclusion, the presence of S. mansoni antigen in different liver fibrosis stages of CHC patients confirming that concomitant schistosome infection aggravates liver disease.
authors.affiliation: a R & D Department, Biotechnology Research Center , New Damietta , Egypt.
authors.forename: Abdelfattah M
authors.initials: AM
authors.lastname: Attallah
authors.affiliation: b Chemistry Department, Faculty of Science , Mansoura University , Mansoura , Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Far
authors.affiliation: c Chemistry Department, Faculty of Science , Helwan University , Cairo , Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Omran
authors.affiliation: d Tropical Medicine Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: Farid
authors.affiliation: a R & D Department, Biotechnology Research Center , New Damietta , Egypt.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Attallah
authors.affiliation: a R & D Department, Biotechnology Research Center , New Damietta , Egypt.
authors.forename: Dalal
authors.initials: D
authors.lastname: Abd-Elaziz
authors.affiliation: a R & D Department, Biotechnology Research Center , New Damietta , Egypt.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: El-Bendary
authors.affiliation: e Pathology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: El-Dosoky
authors.affiliation: a R & D Department, Biotechnology Research Center , New Damietta , Egypt.|f Biochemistry Division, Chemistry Department, Faculty of Science , Minia University , Minia , Egypt.
authors.forename: Hisham
authors.initials: H
authors.lastname: Ismail
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000276:immunology; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000276:immunology* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D012550:Schistosoma mansoni / Q000276:immunology* / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth
keywords: Hepatitis C virus; antigen detection; interferon-γ; liver fibrosis; schistosoma