title: BCL10 is recruited to sites of DNA damage to facilitate DNA double-strand break repair.
abstract: Recent studies have found BCL10 can localize to the nucleus and that this is linked to tumor aggression and poorer prognosis. These studies suggest that BCL10 localization plays a novel role in the nucleus that may contribute to cellular transformation and carcinogenesis. In this study, we show that BCL10 functions as part of the DNA damage response (DDR). We found that BCL10 facilitates the rapid recruitment of RPA, BRCA1 and RAD51 to sites of DNA damage. Furthermore, we also found that ATM phosphorylates BCL10 in response to DNA damage. Functionally, BCL10 promoted DNA double-strand breaks repair, enhancing cell survival after DNA damage. Taken together our results suggest a novel role for BCL10 in the repair of DNA lesions.
authors.affiliation: a Department of Oncology, Faculty of Medicine and Dentistry , University of Alberta , Edmonton , Alberta , Canada.|b Biophysics Department , Faculty of Science, Cairo University , Giza , Egypt.
authors.forename: Ismail Hassan
authors.initials: IH
authors.lastname: Ismail
authors.affiliation: a Department of Oncology, Faculty of Medicine and Dentistry , University of Alberta , Edmonton , Alberta , Canada.
authors.forename: Ashley
authors.initials: A
authors.lastname: Dronyk
authors.affiliation: a Department of Oncology, Faculty of Medicine and Dentistry , University of Alberta , Edmonton , Alberta , Canada.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Hu
authors.affiliation: a Department of Oncology, Faculty of Medicine and Dentistry , University of Alberta , Edmonton , Alberta , Canada.|c Department of Cell Biology , Faculty of Medicine and Dentistry, University of Alberta , Edmonton , Alberta , Canada.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hendzel
authors.affiliation: a Department of Oncology, Faculty of Medicine and Dentistry , University of Alberta , Edmonton , Alberta , Canada.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Shaw
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000074018:B-Cell CLL-Lymphoma 10 Protein; D001665:Binding Sites / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage / Q000502:physiology*; D006801:Humans
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human
keywords: BCL10; BRCA1; HR repair; NF-κB; RNF8/168