title: The presence of cutan limits the interpretation of cuticular chemistry and structure: Ficus elastica leaf as an example.
abstract: Plant cuticles have been traditionally classified on the basis of their ultrastructure, with certain chemical composition assumptions. However, the nature of the plant cuticle may be misinterpreted in the prevailing model, which was established more than 150 years ago. Using the adaxial leaf cuticle of Ficus elastica, a study was conducted with the aim of analyzing cuticular ultrastructure, chemical composition and the potential relationship between structure and chemistry. Gradual chemical extractions and diverse analytical and microscopic techniques were performed on isolated leaf cuticles of two different stages of development (i.e. young and mature leaves). Evidence for the presence of cutan in F. elastica leaf cuticles has been gained after chemical treatments and tissue analysis by infrared spectroscopy and electron microscopy. Significant calcium, boron and silicon concentrations were also measured in the cuticle of this species. Such mineral elements which are often found in plant cell walls may play a structural role and their presence in isolated cuticles further supports the interpretation of the cuticle as the most external region of the epidermal cell wall. The complex and heterogeneous nature of the cuticle, and constraints associated with current analytical procedures may limit the chance for establishing a relationship between cuticle chemical composition and structure also in relation to organ ontogeny.
authors.affiliation: Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Guzmán-Delgado
authors.affiliation: Instituto Superior de Agronomia, Centro de Estudos Florestais, Universidade de Lisboa, Lisboa, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Graça
authors.affiliation: Instituto Superior de Agronomia, Centro de Estudos Florestais, Universidade de Lisboa, Lisboa, Portugal.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Cabral
authors.affiliation: Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Gil
authors.affiliation: Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Madrid, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Fernández
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D030681:Ficus / Q000737:chemistry / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D008903:Minerals / Q000032:analysis; D019441:Plant Epidermis / Q000737:chemistry / Q000648:ultrastructure; D018515:Plant Leaves / Q000737:chemistry / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014885:Waxes / Q000737:chemistry
chemical_list: D008055:Lipids; D008903:Minerals; D014885:Waxes