title: Acute Exercise-Induced Mitochondrial Stress Triggers an Inflammatory Response in the Myocardium via NLRP3 Inflammasome Activation with Mitophagy.
abstract: Increasing evidence has indicated that acute strenuous exercise can induce a range of adverse reactions including oxidative stress and tissue inflammation. However, little is currently known regarding the mechanisms that underlie the regulation of the inflammatory response in the myocardium during acute heavy exercise. This study evaluated the mitochondrial function, NLRP3 inflammasome activation, and mitochondrial autophagy-related proteins to investigate the regulation and mechanism of mitochondrial stress regarding the inflammatory response of the rat myocardium during acute heavy exercise. The results indicated that the mitochondrial function of the myocardium was adaptively regulated to meet the challenge of stress during acute exercise. The exercise-induced mitochondrial stress also enhanced ROS generation and triggered an inflammatory reaction via the NLRP3 inflammasome activation. Moreover, the mitochondrial autophagy-related proteins including Beclin1, LC3, and Bnip3 were all significantly upregulated during acute exercise, which suggests that mitophagy was stimulated in response to the oxidative stress and inflammatory response in the myocardium. Taken together, our data suggest that, during acute exercise, mitochondrial stress triggers the rat myocardial inflammatory response via NLRP3 inflammasome activation and activates mitophagy to minimize myocardial injury.
authors.affiliation: Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China; Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China; Logistics College of People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Miao
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China.
authors.forename: Jingfen
authors.initials: J
authors.lastname: Ma
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xv
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China; Logistics College of People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Hai
authors.identifier: 0000-0003-2781-9457
authors.initials: H
authors.lastname: Bo
authors.affiliation: Logistics College of People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine and Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, China; Laboratory of Physiological Hygiene and Exercise Science, School of Kinesiology, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Li Li
authors.initials: LL
authors.lastname: Ji
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001343:Autophagy / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D019069:Cell Respiration / Q000187:drug effects; D058847:Inflammasomes / Q000187:drug effects / Q000378:metabolism*; D007249:Inflammation / Q000473:pathology*; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D063306:Mitophagy / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010805:Physical Conditioning, Animal; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D000975:Antioxidants; D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; D016877:Oxidants; D025261:Mitochondrial Proton-Translocating ATPases