title: Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae.
abstract: The in-house Carba-NP and Blue-Carba tests were compared using 30 carbapenemase- and 33 non-producing Enterobacteriaceae. Tests were read by three operators. 100% sensitivity was reported for both tests, but Carba-NP was slightly more specific than Blue-Carba (98.9% vs. 91.7%). We describe potential sources of error during tests' preparation and reading.
authors.affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: João
authors.initials: J
authors.lastname: Pires
authors.affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
authors.forename: Regula
authors.initials: R
authors.lastname: Tinguely
authors.affiliation: labormedizinisches zentrum Dr Risch, Department of Medical Microbiology, Bern-Liebefeld, Switzerland.
authors.forename: Bodmer
authors.initials: B
authors.lastname: Thomas
authors.affiliation: Laboratory of Microbiology, A. Manzoni Hospital, Lecco, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Luzzaro
authors.affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland. Electronic address: andrea.endimiani@ifik.unibe.ch.
authors.forename: Andrea
authors.initials: A
authors.lastname: Endimiani
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis; D015373:Bacterial Typing Techniques / Q000379:methods; D001431:Bacteriological Techniques / Q000379:methods*; D015780:Carbapenems / Q000032:analysis / Q000378:metabolism; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors; D001618:beta-Lactamases / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenemases; Carbapenems; Diagnostics; Klebsiella; MBL; MIC; NDM; OXA-48; VIM