title: Mass spectrometric profiling of lipids in intestinal tissue from rats fed cereals processed for medical conditions.
abstract: Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used for lipid profiling of intestine tissue sections from rats fed specially processed cereals and rats fed ordinary feed as a control. This cereal is known to increase the activity of antisecretory factor in plasma and the exact mechanism for the activation process at the cellular level is unclear. ToF-SIMS has been used to track food induced changes in lipid content in intestinal tissue sections to gain insight into the possible mechanisms involved. Data from 20 intestine sections belonging to four different rats from each group of control and specially processed cereals-fed rats were obtained using the stage scan macroraster with a lateral resolution of 5 μm. Data were subsequently subjected to orthogonal partial least squares discriminant analysis. The data clearly show that changes of certain lipids are induced by the specially processed cereal feed. Scores plots show a well-defined separation between the two groups. The corresponding loading plots reveal that the groups separate mainly due to changes of vitamin E, phosphocholine, and phosphosphingolipid fragments, and that for the c18:2 fatty acid. The observed changes in lipids might give insight into the working mechanisms of antisecretory factor in the body, and this has been successfully used to understand the working mechanism of specially processed cereal-induced antisecretory factor activation in intestine.
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden and National Center for Imaging Mass Spectrometry, Gothenburg 412 96, Sweden.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Dowlatshahi Pour
authors.affiliation: Institute of Biomedicine, Gothenburg 413 90, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Jennische
authors.affiliation: Institute of Biomedicine, Gothenburg 413 90, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lange
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden; National Center for Imaging Mass Spectrometry, Gothenburg 412 96, Sweden; and Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 412 96, Sweden.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Ewing
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden and National Center for Imaging Mass Spectrometry, Gothenburg 412 96, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Malmberg
mesh_terms: D000821:Animal Feed; D000818:Animals; D004032:Diet / Q000379:methods*; D002523:Edible Grain; D007422:Intestines / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D051381:Rats; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods
chemical_list: D008055:Lipids