title: Interneurons contribute to the hemodynamic/metabolic response to epileptiform discharges.
abstract: Interpretation of hemodynamic responses in epilepsy is hampered by an incomplete understanding of the underlying neurovascular coupling, especially the contributions of excitation and inhibition. We made simultaneous multimodal recordings of local field potentials (LFPs), firing of individual neurons, blood flow, and oxygen level in the somatosensory cortex of anesthetized rats. Epileptiform discharges induced by bicuculline injections were used to trigger large local events. LFP and blood flow were robustly coupled, as were LFP and tissue oxygen. In a parametric linear model, LFP and the baseline activities of cerebral blood flow and tissue partial oxygen tension contributed significantly to blood flow and oxygen responses. In an analysis of recordings from 402 neurons, blood flow/tissue oxygen correlated with the discharge of putative interneurons but not of principal cells. Our results show that interneuron activity is important in the vascular and metabolic responses during epileptiform discharges.
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France;
authors.forename: Sandrine
authors.initials: S
authors.lastname: Saillet
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France;
authors.forename: Pascale P
authors.initials: PP
authors.lastname: Quilichini
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France;
authors.forename: Antoine
authors.initials: A
authors.lastname: Ghestem
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France; APHM, Timone Hospital, Division of Public Health, Marseille, France;
authors.forename: Bernard
authors.initials: B
authors.lastname: Giusiano
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France;
authors.forename: Anton I
authors.initials: AI
authors.lastname: Ivanov
authors.affiliation: INRIA, Project-team Athena, Sophia-Antipolis, France; and.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hitziger
authors.affiliation: Aix-Marseille Université, CNRS, INT UMR 7289, Marseille, France.
authors.forename: Ivo
authors.initials: I
authors.lastname: Vanzetta
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France;
authors.forename: Christophe
authors.initials: C
authors.lastname: Bernard
authors.affiliation: INSERM, UMR 1106, Marseille, France; Aix-Marseille Université, Institut de Neurosciences des Systèmes, Marseille, France; christian.benar@univ-amu.fr.
authors.forename: Christian-G
authors.initials: CG
authors.lastname: Bénar
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000633:toxicity; D002560:Cerebrovascular Circulation; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology*; D005073:Evoked Potentials, Somatosensory; D007395:Interneurons / Q000378:metabolism / Q000502:physiology*; D008297:Male; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000098:blood supply / Q000166:cytology / Q000503:physiopathology*
chemical_list: D001640:Bicuculline
keywords: action potentials; animal models; cerebral hemodynamics; electrophysiology; epilepsy; neurovascular coupling; oxygen