title: Leeuwenhoekiella nanhaiensissp. nov., isolated from deep-sea water.
abstract: A novel heterotrophic, aerobic, Gram-stain-negative, rod-shaped and yellow bacterium, designated strain G18T, was isolated from a water sample collected from the deep South China Sea. Strain G18Tgrew at 4-40 °C (optimum 28-32 °C), at pH 6.0-8.0 (optimum pH 6.5-7.5) and with 0-12 % (w/v) NaCl (optimum 3-4 %). The organism was mesophilic and piezotolerant, its optimal growth pressure was 0.1 MPa, which was lower than that at the depth from which it was isolated. Its optimal growth temperature was higher than that at the depth of its isolation. The predominant cellular fatty acids were C15 : 0iso, C17 : 0iso 3-OH and C15 : 1iso. The major polar lipids were composed of phosphatidylethanolamine, one unknown aminolipid and one unknown polar lipid. The major respiratory quinone was menaquinone 6. The G+C content of the genomic DNA was 35 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain G18Tclustered with species of the genusLeeuwenhoekiellawith validly published names within the familyFlavobacteriaceaewith 95.9-98.2 % sequence similarity. DNA-DNA reassociation values ranged from 9 to 42 %. Differential phenotypic properties, together with the phylogenetic distinctiveness, suggest that strain G18Tdiffers from species of the genusLeeuwenhoekiellawith validly published names. On the basis of the polyphasic evidence, strain G18Trepresents a novel species, isolated from deep-sea, of the genusLeeuwenhoekiellafor which the nameLeeuwenhoekiella nanhaiensissp. nov. is proposed. The type strain is G18T( = CCTCC AB 2015204T= KCTC 42729T).
authors.affiliation: State Key Laboratory of Marine Geology, Tongji University,1239 Siping Road, Shanghai, 200092,PR China.
authors.forename: Qianfeng
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Geology, Tongji University,1239 Siping Road, Shanghai, 200092,PR China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Geology, Tongji University,1239 Siping Road, Shanghai, 200092,PR China.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University,Jinan 250100,PR China.
authors.forename: Xiying
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Geological Processes and Mineral Resources, Faculty of Earth Sciences, China University of Geosciences,Wuhan, Hubei 430074,PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University,Jinan 250100,PR China.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hadal Science and Technology Research Center, Shanghai Ocean University,999 Huchenghuan Road, Shanghai 201306,PR China.|College of Natural and Computational Sciences, Hawaii Pacific University,Kaneohe, HI 96744,USA.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Fang