title: Amino-Functionalized Ceramic Capillary Membranes for Controlled Virus Retention.
abstract: A straightforward chemical functionalization strategy using aminosilanes for high-flux yttria-stabilized zirconia capillary membranes is presented (macroporous, d50 = 144 nm, open porosity =49%, membrane flux ∼150 L/(m(2)hbar)). Three different aminosilanes with one, two or three amino groups per silane molecule, namely 3-aminopropyltriethoxysilane (APTES), N-(2-aminoethyl)-3-aminopropyltriethoxysilane (AE-APTES) and N-(3-trimethoxysilylpropyl)diethylenetriamine (TPDA), are used to generate the amino-functionalized membranes. With a higher number of amino groups per silane molecule increased loading capacities between 0.44 and 1.01 accessible amino groups/nm(2) membrane are achieved. Streaming potential measurements confirm that the zeta-potential of the membrane surface is converted from negative (non-functionalized) to positive (amino-functionalized). By operation in dead-end filtration mode using the model virus MS2 (diameter = 25 nm, IEP = 3.9) the virus retention capacity of the amino-functionalized membranes is significantly increased and log reduction values (LRVs) of up to 9.6 ± 0.3 (TPDA) are obtained whereas a LRV < 0.3 is provided by the non-functionalized membranes. Long-term dead-end filtration experiments for 1 week reveal a high stability of immobilized aminosilanes (TPDA), being robust against leaching. By iterative backflushing with desorption buffer MS2-loaded membranes are successfully regenerated being reusable for a new filtration cycle. The presented functionalization platform is highly promising for controlled virus retention.
authors.affiliation: Advanced Ceramics, University of Bremen , Am Biologischen Garten 2, 28359 Bremen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Bartels
authors.affiliation: Advanced Ceramics, University of Bremen , Am Biologischen Garten 2, 28359 Bremen, Germany.
authors.forename: Marina N
authors.initials: MN
authors.lastname: Souza
authors.affiliation: Advanced Ceramics, University of Bremen , Am Biologischen Garten 2, 28359 Bremen, Germany.
authors.forename: Amelie
authors.initials: A
authors.lastname: Schaper
authors.affiliation: Institute of Electronic- and Sensor-Materials, Technical University (TU) Bergakademie Freiberg , Gustav-Zeuner Str. 3, 09599 Freiberg, Germany.
authors.forename: Pál
authors.initials: P
authors.lastname: Árki
authors.affiliation: Advanced Ceramics, University of Bremen , Am Biologischen Garten 2, 28359 Bremen, Germany.|MAPEX - Center for Materials and Processes, University of Bremen , Am Fallturm 1, 28359 Bremen, Germany.
authors.forename: Stephen
authors.initials: S
authors.lastname: Kroll
authors.affiliation: Advanced Ceramics, University of Bremen , Am Biologischen Garten 2, 28359 Bremen, Germany.|MAPEX - Center for Materials and Processes, University of Bremen , Am Fallturm 1, 28359 Bremen, Germany.
authors.forename: Kurosch
authors.initials: K
authors.lastname: Rezwan
mesh_terms: D002516:Ceramics; D005374:Filtration; D008567:Membranes, Artificial; D016062:Porosity; D012821:Silanes / Q000737:chemistry*; D014780:Viruses; D014871:Water Microbiology
chemical_list: D008567:Membranes, Artificial; D012821:Silanes