title: Elevation of Brain Magnesium Potentiates Neural Stem Cell Proliferation in the Hippocampus of Young and Aged Mice.
abstract: In the adult brain, neural stem cells (NSCs) can self-renew and generate all neural lineage types, and they persist in the sub-granular zone (SGZ) of the hippocampus and the sub-ventricular zone (SVZ) of the cortex. Here, we show that dietary-supplemented - magnesium-L-threonate (MgT), a novel magnesium compound designed to elevate brain magnesium regulates the NSC pool in the adult hippocampus. We found that administration of both short- and long-term regimens of MgT, increased the number of hippocampal NSCs. We demonstrated that in young mice, dietary supplementation with MgT significantly enhanced NSC proliferation in the SGZ. Importantly, in aged mice that underwent long-term (12-month) supplementation with MgT, MgT did not deplete the hippocampal NSC reservoir but rather curtailed the age-associated decline in NSC proliferation. We further established an association between extracellular magnesium concentrations and NSC self-renewal in vitro by demonstrating that elevated Mg(2+) concentrations can maintain or increase the number of cultured hippocampal NSCs. Our study also suggests that key signaling pathways for cell growth and proliferation may be candidate targets for Mg(2+) 's effects on NSC self-renewal. J. Cell. Physiol. 231: 1903-1912, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jia
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yihe
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Meiyan
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
mesh_terms: D000375:Aging; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D008274:Magnesium