title: Enhancing the growth rate and astaxanthin yield of Haematococcus pluvialis by nuclear irradiation and high concentration of carbon dioxide stress.
abstract: Unicellular green microalgae Haematococcus pluvialis was mutated with (60)Co-γ irradiation to promote growth rate and increase astaxanthin yield under high concentration of CO2 stress. The average specific growth rate of H. pluvialis mutated with 4000 Gy γ-ray irradiation was increased by 15% compared with the original strain with air aeration. The mutant grew best with 6% CO2 (the maximum specific growth rate was 0.60/d) when it was cultured with high concentrations of CO2 (2-10%). The peak biomass productivity (0.16 g/L/d) of the mutant cultured with 6% CO2 was 82% higher than that of the mutant with air. The astaxanthin yield and lipid content of the mutant induced with 6% CO2 and high light (108 μmol photons m(-2) s(-1)) increased to 46.0mg/L and 45.9%, which were 2.4 and 1.3 times higher than those of the wild-type strain, respectively.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Zongbo
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology*; D018929:Cell Culture Techniques; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D005720:Gamma Rays; D007218:Industrial Microbiology / Q000379:methods; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D024341:Xanthophylls / Q000378:metabolism
chemical_list: D024341:Xanthophylls; D002245:Carbon Dioxide; C005948:astaxanthine
keywords: Astaxanthin; Growth rate; Haematococcus pluvialis; High CO(2); Nuclear mutagenesis