title: Distinct RNA transcriptome patterns are potentially associated with angiogenesis in Tie2-expressing monocytes.
abstract: Tie2-expressing Monocytes (TEMs) were previously identified as a novel subset of monocytes and were believed to have prominent pro-angiogenesis activities in human tumors. While the molecular mechanism of the angiogenesis promoting capacity of TEMs remains unclear. RNA transcriptome pattern, including non-coding RNAs as microRNA (miRNA) and long non-coding RNA (lncRNA), plays important role in cell differentiation and functions. However, little is known about the transcriptome patterns of TEMs, including those non-coding RNAs. We explore the transcriptome of TEMs and the matched monocytes that do not express Tie2 (Tie2(-)monocytes) isolated from peripheral blood of healthy adults employing the Agilent Human miRNA(8*60K,Design ID: 046064)microarray and the Agilent lncRNA Gene Expression(4*180K, Design ID: 042818)microarray. A total of 141 mRNAs, 142 lncRNAs and 75 miRNAs were found dysregulated in TEMs compared to Tie2(-)monocytes. TEMs have the distinct RNA transcriptome patterns according to the Hierarchical clustering and then the gene expression patterns were confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Functional annotation by Gene Ontology (GO) analyses showed that the up-regulated mRNAs in TEMs were associated to blood vessel remodeling and positive regulation of epithelial cell proliferation, and the up-regulated insulin like growth factor 1(IGF1) mRNA was involved in both pathways. For functional analysis of those dysregulated non-coding RNAs, target genes of the miRNAs were predicted and cis/trans-regulation analysis of the lncRNAs were performed.
authors.affiliation: Shanghai First Maternity and Infant Hospital, Tongji University, School of Medicine. Electronic address: wxj2007@hotmail.com.
authors.forename: Xinjing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shanghai First Maternity and Infant Hospital, Tongji University, School of Medicine. Electronic address: dzypaper@hotmail.com.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Shanghai First Maternity and Infant Hospital, Tongji University, School of Medicine. Electronic address: smalllily2012@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Shanghai First Maternity and Infant Hospital, Tongji University, School of Medicine. Electronic address: kaiwangcn@yahoo.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Shanghai First Maternity and Infant Hospital, Tongji University, School of Medicine. Electronic address: Dr_xwang@hotmail.com.
authors.forename: Xipeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009369:Neoplasms / Q000098:blood supply / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D042787:Receptor, TIE-2 / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome / Q000235:genetics
chemical_list: C583557:IGF1 protein, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D042787:Receptor, TIE-2
keywords: Cis/trans-regulation; Microarray; lncRNA; miRNA