title: Classic Analysis of Biopolymer Dynamics Is Model Free.
abstract: Early analysis of biopolymer dynamics relied on a variety of motional models that were difficult to distinguish and sometimes gave contradictory results. The Lipari-Szabo model-free approach (documented in a 1980 article in Biophysical Journal, as well as in two more comprehensive 1982 articles in the Journal of the American Chemical Society, provided a simple formalism that allowed investigators to understand fluorescence and NMR experimental data without having to specify a motional model. Although the model-free method is not universally applicable (for example, its assumption of a uniform correlation time for overall molecular tumbling can be problematic for biomolecules containing areas of disorder), it remains the most popular and widely used technique for analyzing molecular motion.
authors.affiliation: Department of Integrative Structural and Computational Biology MB202, The Scripps Research Institute, La Jolla, California. Electronic address: fenwick@scripps.edu.
authors.forename: R Bryn
authors.initials: RB
authors.lastname: Fenwick
authors.affiliation: Department of Integrative Structural and Computational Biology MB202, The Scripps Research Institute, La Jolla, California. Electronic address: dyson@scripps.edu.
authors.forename: H Jane
authors.initials: HJ
authors.lastname: Dyson
mesh_terms: D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D009068:Movement
chemical_list: D001704:Biopolymers