title: Silk fibroin nanoparticles support in vitro sustained antibiotic release and osteogenesis on titanium surface.
abstract: Increasing amounts of metal-based implants are used for orthopedic or dental surgeries throughout the world. Still several implant-related problems such as inflammation, loosening and bacterial infection are prevalent. These problems stem from the immediate microbial contamination and failure of initial osteoblast adhesion. Additionally, bacterial infections can cause serious and life-threatening conditions such as osteomyelitis. Here, antibiotic (gentamicin)-loaded silk protein fibroin (non-mulberry silkworm, Antheraea mylitta) nanoparticles are fabricated and deposited over the titanium surface to achieve sustained drug release in vitro and to alter the surface nano-roughness. Based on the altered surface topography, chemistry and antibacterial activity, we conclude that the nanoparticle-deposited surfaces are superior for osteoblast adhesion, proliferation and differentiation in comparison to bare Ti. This method can be utilized as a cost-effective approach in implant modification.
  
  
  
  Titanium-based implants are commonly used in the field of orthopedics or dentistry. Surface modification of an implant is vital to ensure osseointegration. In this article, the author investigated the use of silk protein fibroins for metal surface modification and also for drug delivery against bacteria. The encouraging data should provide a new method in terms of nanotechnology in the respective clinical fields.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India.
authors.forename: Shaily
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India.
authors.forename: Subia
authors.initials: S
authors.lastname: Bano
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India.
authors.forename: Anindya S
authors.initials: AS
authors.lastname: Ghosh
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
authors.forename: Mahitosh
authors.initials: M
authors.lastname: Mandal
authors.affiliation: Institute of Tissue Regeneration Engineering and Department of Nanobiomedical Science BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, South Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India. Electronic address: tulidey@unipune.ac.in.
authors.forename: Tuli
authors.initials: T
authors.lastname: Dey
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India. Electronic address: kundu@hijli.iitkgp.ernet.in.
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000900:Anti-Bacterial Agents; D016503:Drug Delivery Systems; D005349:Fibroins; D006801:Humans; D053758:Nanoparticles; D010012:Osteogenesis; D013499:Surface Properties; D014025:Titanium
chemical_list: D000900:Anti-Bacterial Agents; D005349:Fibroins; D014025:Titanium
keywords: Controlled drug release; Fibroin; Nanoparticle; Osteogenesis; Titanium