title: A six-microRNA set as prognostic indicators for bile duct cancer.
abstract: MicroRNAs (miRNAs) play important roles in cancer progression by altering transcriptional control. The purpose of this study is to identify and explore specific miRNAs as prognostic and predictive biomarkers for bile duct cancer (BDC) by analyzing Next-generation data. miRNA expression profiles and corresponding clinical information of BDC samples were extracted from The Cancer Genome Atlas (TCGA). The differentially expressed miRNAs were determined by SAMR package in R software. Target genes of those miRNAs were predicted by Targetscan. Functional enrichment analysis and hypergeometric test analysis of target genes were performed. Then, diagnosis accuracy of miRNAs was judged by ROC Curves analysis. Total 120 differentially expressed miRNAs were obtained, of which six important miRNAs were selected and predicted as prognosis and predicting biomarkers in BDC. Besides, functional analysis showed that both enriched pathways were significantly related with ion binding, which might involve in the carcinogenesis of BDC. Moreover, top 3 important pathways sharing the most influence were noted. Our results demonstrated that hsa-miR-483-5p, hsa-miR-675, hsa-miR-139-3p, hsa-miR-598, hsa-miR-625 and hsa-miR-187 could serve as prognostic and predictive markers for survival of BDC patients and could potentially be provided as targets for future therapy.
authors.affiliation: Department of Hepatic Surgery, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Hepatic Surgery, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Tian-Fu
authors.initials: TF
authors.lastname: Wen
authors.affiliation: Department of General Surgery, Xishuangbanna Prefecture People's Hospital Xishuangbanna 666100, China.
authors.forename: Lin-Hai
authors.initials: LH
authors.lastname: He
authors.affiliation: Department of Hepatic Surgery, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Hepatic Surgery, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Wen-Jiang
authors.initials: WJ
authors.lastname: Zhu
authors.affiliation: Department of Hepatic Surgery, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Narasimha Murthy
authors.initials: NM
authors.lastname: Trishul
keywords: Bile duct cancer; functional enrichment; microRNA; pathway analysis; target gene