title: [Determination of sugars, organic acids and alcohols in microbial consortium fermentation broth from cellulose using high performance liquid chromatography].
abstract: A high performance liquid chromatographic method was established for the determination of metabolites (sugars, organic acids and alcohols) in microbial consortium fermentation broth from cellulose. Sulfate was first added in the samples to precipitate calcium ions in microbial consortium culture medium and lower the pH of the solution to avoid the dissociation of organic acids, then the filtrates were effectively separated using high performance liquid chromatography. Cellobiose, glucose, ethanol, butanol, glycerol, acetic acid and butyric acid were quantitatively analyzed. The detection limits were in the range of 0.10-2.00 mg/L. The linear correlation coefficients were greater than 0.999 6 in the range of 0.020 to 1.000 g/L. The recoveries were in the range of 85.41%-115.60% with the relative standard deviations of 0.22% -4.62% (n = 6). This method is accurate for the quantitative analysis of the alcohols, organic acids and saccharides in microbial consortium fermentation broth from cellulose.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Guifang
authors.initials: G
authors.lastname: Fan
authors.forename: Ran
authors.initials: R
authors.lastname: Du
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000143:Acids / Q000032:analysis*; D000438:Alcohols / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D057230:Limit of Detection; D059013:Microbial Consortia; D009930:Organic Chemicals / Q000032:analysis
chemical_list: D000143:Acids; D000438:Alcohols; D002241:Carbohydrates; D003470:Culture Media; D009930:Organic Chemicals; D002482:Cellulose