title: Nylon mesh filtration technique for the collection of metacercariae from host tissue by digestion with artificial gastric juice.
abstract: The traditional method of collecting parasite larvae by digestion with artificial gastric juice requires time-consuming processes of repeated sedimentation and washing that is likely to result in a loss of some of the larvae. The author introduces an improved filtration technique using nylon mesh to wash out the digestive fluid. The technique shortens the processing duration of the process and increases the efficiency of larva collection.
authors.affiliation: Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan. Electronic address: kino624@hama-med.ac.jp.
authors.forename: Hideto
authors.initials: H
authors.lastname: Kino
keywords: Digestion method; Metacercariae; Nylon mesh filtration technique