title: Ehrlichia minasensissp. nov., isolated from the tickRhipicephalus microplus.
abstract: Recently, we obtained a rickettsial isolate (Ehrlichiasp. UFMG-EVT) from the haemolymph of engorgedRhipicephalus microplustick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene,groEL,dsb,gltAandtrp36sequences we showed thatEhrlichiasp. UFMG-EVTbelongs to theα-Proteobacteria, familyAnaplasmataceae, genusEhrlichia.Ehrlichiasp. UFMG-EVTis a sister taxon ofEhrlichia caniswith 16S rRNA gene,groEL,dsb,gltAandtrp36sequence similarities of 98.3 %, 97.2 %, 94.7 %, 94.3 % and 49.1 %, respectively.Ehrlichiasp. UFMG-EVThas been maintained in the laboratory by continuous passage in the IDE8 tick cell line where the ultrastructure was characterized using electron microscopy and was found to resemble that ofE. canis,Ehrlichia murisandEhrlichia chaffeensis, but notEhrlichia ruminantiumandEhrlichia ewingii. We propose the nameEhrlichia minasensissp. nov. for this bacterium to acknowledge the place from where it was initially isolated, Minas Gerais, Brazil. The type strain is strainEhrlichiasp. UFMG-EVT( = DSM 100393T= TCB-TBB-0018T).
authors.affiliation: Center for Infection and Immunity of Lille (CIIL), INSERM U1019 - CNRS UMR 8204, Université de Lille, Institut Pasteur de Lille, 1 rue Professeur Calmette, 59019 Lille Cedex, France.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cabezas-Cruz
authors.affiliation: Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstrasse 5, 80802 Munich, Germany.|Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa.
authors.forename: Erich
authors.initials: E
authors.lastname: Zweygarth
authors.affiliation: University of South Bohemia, Faculty of Science and Biology Centre of the ASCR, Institute of Parasitology, Cˇeské Budeˇjovice, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Vancová
authors.affiliation: Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstrasse 5, 80802 Munich, Germany.
authors.forename: Marzena
authors.initials: M
authors.lastname: Broniszewska
authors.affiliation: University of South Bohemia, Faculty of Science and Biology Centre of the ASCR, Institute of Parasitology, Cˇeské Budeˇjovice, Czech Republic.
authors.forename: Libor
authors.initials: L
authors.lastname: Grubhoffer
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária-UFMG, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Lygia Maria Friche
authors.initials: LMF
authors.lastname: Passos
authors.affiliation: Departamento de Parasitologia, ICB-UFMG, Belo Horizonte, Brazil.
authors.forename: Múcio Flávio Barbosa
authors.initials: MFB
authors.lastname: Ribeiro
authors.affiliation: SaBio. Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Alberdi
authors.affiliation: SaBio. Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain.|Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente