title: Bioactive lignan derivatives from the stems of Firmiana simplex.
abstract: The CHCl3 soluble fraction of the 80% MeOH extract of the stems of Firmiana simplex strongly inhibited nitric oxide production in lipopolysaccharide-activated BV-2 cells. A bioactivity-guided column chromatographic separation yielded two new lignans, firmianols A and B (1-2) together with seventeen known lignans (3-19). The structural elucidation of the new compounds was determined by spectroscopic methods, including 1D, 2D NMR and HR-FAB-MS. All isolated lignans were evaluated for their antineuroinflammatory effects on nitric oxide (NO) production in lipopolysaccharides (LPS)-activated murine microglia BV2 cells. Among the isolated, compounds 14 and 15 showed potent inhibitory activity against NO production (IC50 1.05 and 0.929 μM, respectively) without cell toxicity in murine microglia BV-2 cells. Compounds 11-13 and 17 also exhibited strong inhibitory effects on NO production, with IC50 values ranging from 7.07 to 15.28 μM.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 2066 Seobu-Ro, Jangan-ku, Suwon, Gyeonggi-do, Republic of Korea.
authors.forename: Kyeong Wan
authors.initials: KW
authors.lastname: Woo
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 2066 Seobu-Ro, Jangan-ku, Suwon, Gyeonggi-do, Republic of Korea.
authors.forename: Won Se
authors.initials: WS
authors.lastname: Suh
authors.affiliation: College of Pharmacy, Gachon University, #191 Hambakmoe-ro, Yeonsu-gu, Incheon 406-799, Republic of Korea.
authors.forename: Lalita
authors.initials: L
authors.lastname: Subedi
authors.affiliation: College of Pharmacy, Gachon University, #191 Hambakmoe-ro, Yeonsu-gu, Incheon 406-799, Republic of Korea; Gachon Medical Research Institute, Gil Medical Center, Incheon, Republic of Korea.
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.affiliation: The Graduate School of Alternative Medicine, Kyonggi University, Seoul, Republic of Korea.
authors.forename: Aejung
authors.initials: A
authors.lastname: Kim
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 2066 Seobu-Ro, Jangan-ku, Suwon, Gyeonggi-do, Republic of Korea. Electronic address: krlee@skku.edu.
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D019660:Malvaceae / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry / Q000235:genetics
chemical_list: D000893:Anti-Inflammatory Agents; D017705:Lignans; D008070:Lipopolysaccharides; D010936:Plant Extracts; C000611486:firmianol A; C000611487:firmianol B; D009569:Nitric Oxide
keywords: Anti-neuroinflammatory effect; Firmiana simplex; Lignan; Sterculiaceae