title: Effects of diclofenac sodium and octreotide on treatment of caerulein-induced acute pancreatitis in mice.
abstract: BACKGROUND
  Research continues to develop novel therapeutic modalities that particularly focus on the pathogenesis of acute pancreatitis. This study aimed to assess the effects of diclofenac sodium and octreotide, alone or in combination, on pancreatic enzymes, pancreatic myeloperoxidase activity, histopathology and apoptosis of pancreas cells, using a model of experimentally induced acute pancreatitis.
  
  
  OBJECTIVE
  We aimed to demonstrate effects of diclofenac sodium, octreotide and their combined use on pancreatic enzymes, activity of pancreatic myeloperoxidase (MPO) activity, histopathology and apoptosis of pancreas on treatment of caerulin-induced experimental acute pancreatitis.
  
  
  METHODS
  Caerulin-induced acute pancreatitis model was created using a total of 58 male BALB-C mice of 25 gr in seven groups. Serum amylase, lipase levels and pancreatic myeloperoxidase activity were examined as well as apoptotic values in pancreatic acinar cells through TUNNEL method. Histopathology of pancreas was evaluated for presence of edema, hemorrhage, parenchymal necrosis, fat necrosis, leukocyte infiltration, and fibrosis.
  
  
  RESULTS
  In the diclofenac sodium group, apoptotic values in the pancreatic acinar cells were found to be statistically lower than in the acute pancreatitis group in terms of parenchymal necrosis and hemorrhage scores (P = 0.007, P = 0.002, and P = 0.052, respectively). No statistically significant differences were found in serum level of amylase, lipase, pancreatic myeloperoxidase activity and the other histopathological scores (P > 0.05).
  
  
  CONCLUSIONS
  Diclofenac sodium, a cost-effective agent with a favorable side-effect profile, may represent a novel therapeutic agent for the treatment of acute pancreatitis. Findings of this study suggest a better efficacy for diclofenac sodium monotherapy as compared to octreotide alone or octreotide/diclofenac combination.
authors.affiliation: Department of Gastroenterology and Hepatology, Konya Education and Research Hospital Konya, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ozer Cakir
authors.affiliation: Department of Pathology, Meram School of Medicine, Necmettin Erbakan University Konya, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Esen
authors.affiliation: Department of Biochemistry, Meram School of Medicine, Necmettin Erbakan University Konya, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Toker
authors.affiliation: Department of Gastroenterology and Hepatology, Meram School of Medicine, Necmettin Erbakan University Konya, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ataseven
authors.affiliation: Department of Gastroenterology and Hepatology, Meram School of Medicine, Necmettin Erbakan University Konya, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Demir
authors.affiliation: Department of Gastroenterology and Hepatology, Meram School of Medicine, Necmettin Erbakan University Konya, Turkey.
authors.forename: Hakki
authors.initials: H
authors.lastname: Polat
keywords: Diclofenac sodium; experimental acute pancreatitis; octreotide