title: MicroRNA-103 suppresses tumor cell proliferation by targeting PDCD10 in prostate cancer.
abstract: BACKGROUND
  It is known that microRNAs (miRNAs) are a class of small, non-coding RNAs that act as key regulators in various physiological and pathological processes. However, the regulatory mechanisms involving miRNAs in prostate cancer remain largely unknown. Here, we found that miR-103 is down-regulated in prostate cancer and closely associated with tumor proliferation and migration. Our objective was to explore the role of the miR-103 in prostate cancer.
  
  
  METHODS
  In this study, we measured miR-103 level using real-time polymerase chain reaction in the human prostate cancer cell lines, including PC-3, LNCap, 22Rv1, DU145, and the normal prostate epithelium cell line RWPE-1, a total of 25 pairs of primary prostate cancer tissues and adjacent non-cancerous tissues (NCTs) were measured also. In addition, over-expression of miR-103 in prostate cancer cell lines to determine the role of miR-103 in prostate cancer.
  
  
  RESULTS
  We found that miR-103 is down-regulated in prostate cancer and closely associated with tumor proliferation and migration. In addition, over-expression of miR-103 apparently inhibits prostate cancer cell proliferation and migration in vitro. Gain-of-function in vitro experiments further show that miR-103 mimics significantly inhibited prostate cancer cell proliferation, invasion and increase the cell cycle in G1 phase, while promoted cell apoptosis. Subsequent dual-luciferase reporter assay identified one of the proto-oncogene PDCD10 as direct target of miR-103.
  
  
  CONCLUSIONS
  Therefore, our data collectively demonstrate that miR-103 is a proto-oncogene miRNA that can suppress prostate cancer proliferation and migration by down-regulating the oncogene PDCD10, indicating that miR-103 may represent a new potential diagnostic and therapeutic target for prostate cancer treatment.
authors.affiliation: Departmentof Urology, Xi'an Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Fu
authors.affiliation: Departmentof Urology, Xi'an Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Chinese People's Liberation Army 323 hospital, Xi'an, China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Su
authors.affiliation: Armed Police Hospital of Shaanxi, Xi'an, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Departmentof Urology, Xi'an Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Departmentof Urology, Xi'an Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: He
authors.initials: H
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D051017:Apoptosis Regulatory Proteins; C000715470:MAS1 protein, human; C544749:MIRN103 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C495390:PDCD10 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins
keywords: PDCD10; miR-103; migration; proliferation; prostate cancer