title: [Antibiotics-resistance and pulsed-field gel electrophoresis patterns of Shigella sonnei from different regions].
abstract: OBJECTIVE
  To understand the epidemic tendency and antibiotics-resistance among Shigella sonnei isolates collected from different regions by antibiotic susceptibility testing, PCR amplification of the resistance genes and genotyping.
  
  
  METHODS
  The susceptibilities to 21 antibiotics of 54 S. sonnei strains were determined by broth microdilution using a 96-well microtiter plate. The amplification of resistance genes was performed by PCR. Pulsed field gel electrophoresis genotyping method was applied to analyze their genetic relationships, and BioNumerics software was used to analyze the PFGE patterns.
  
  
  RESULTS
  All tested S. sonnei strains were resistant to Trimethoprim/Sulfamethoxazole, Tetracycline, Ticarcillin, Ampicillin and Gentamicin, whereas sensitive to Imipenem, Cefepime, Levofloxacin, Norfloxacin and Amikacin. A total of 7 different antibiotic resistance genes including blaTEM, blaCTX and intI were identified in the multidrug-resistant S. sonneis strains. PFGE patterns of all the isolates showed a high genetic homology.
  
  
  CONCLUSIONS
  It is of great importance to strengthen the surveillance of S. sonnei from different regions in order to reduce the prevalence of multidrug-resistant strains.
authors.forename: Beibei
authors.initials: B
authors.lastname: Liang
authors.forename: Shaofu
authors.initials: S
authors.lastname: Qiu
authors.forename: Xianyan
authors.initials: X
authors.lastname: Cui
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Shengjie
authors.initials: S
authors.lastname: Yi
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Yang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ren
authors.forename: Hongbin
authors.initials: H
authors.lastname: Song
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China; D024881:Drug Resistance, Bacterial; D004405:Dysentery, Bacillary / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D012764:Shigella sonnei / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents