title: Synthesis and Characterisation of Biocompatible Polymer-Conjugated Magnetic Beads for Enhancement Stability of Urease.
abstract: We reported natural polymer-conjugated magnetic featured urease systems for removal of urea effectively. The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), operational stability (0-250 min), reusability (18 times) and storage stability (24 weeks) were studied for characterisation of the urease-encapsulated biocompatible polymer-conjugated magnetic beads. Also, the surface groups and chemical structure of the magnetic beads were determined by using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The all urease-encapsulated magnetic beads protected their stability of 30-45 % relative activity at 70 °C. A significant increase was observed at their pH stability compared with the free urease for both acidic and alkaline medium. Besides this, their repeatability activity were approximately 100 % during 4(th) run. They showed residual activity of 50 % after 16 weeks. The importance of this work is enhancement stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for the industrial application based on removal of urea.
authors.affiliation: Faculty of Science, Chemistry Department, Muğla Sıtkı Koçman University, Muğla, Turkey. y-ispirli@hotmail.com.
authors.forename: Yasemin Ispirli
authors.initials: YI
authors.lastname: Doğaç
authors.affiliation: Faculty of Science, Chemistry Department, Muğla Sıtkı Koçman University, Muğla, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Teke
mesh_terms: D001704:Biopolymers / Q000737:chemistry / Q000235:genetics*; D048271:Chitosan / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D060328:Magnetic Phenomena; D013696:Temperature; D014508:Urea / Q000737:chemistry*; D014510:Urease / Q000737:chemistry* / Q000235:genetics
chemical_list: D001704:Biopolymers; D004800:Enzymes, Immobilized; D014508:Urea; D048271:Chitosan; D014510:Urease
keywords: Fe3O4 nanoparticles; Fe[NiFe]O4 nanoparticles; Magnetic polymeric beads; Urease encapsulation; Urease stability