title: Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response.
abstract: Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genespi21in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker geneOsWRKY45of salicylic acid (SA) signalling was upregulated in all tested cultivars. And,JAmyb(marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR)RgenesPib,Pizt,Pik,Pita2andPikaheithan in NB and KHS.SalTof abscisic acid (ABA) signalling may be involved in theR/Avrinteraction, includingPizt,Pik,pi21andPikahei. However,SalTwas shown to negatively regulatePib/AvrPibinteraction.OsPR1bandPBZ1were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence thatOsPR1bandPBZ1played an important role in thepi21-mediated response. It was shown thatOsRAR1could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, whileOsSGT1andOsHSP90could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
authors.forename: Debing
authors.initials: D
authors.lastname: Wang
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, P.R. China; College of Life Science, South China Agricultural University, Guangzhou, Guangdong, P.R. China.
authors.forename: Jingluan
authors.initials: J
authors.lastname: Han
authors.affiliation: Agricultural Technology Extension and Service Center of Jingzhou City , Jingzhou , Hubei , P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
authors.forename: Xuequn
authors.initials: X
authors.lastname: Liu
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
authors.forename: Chuntai
authors.initials: C
authors.lastname: Wang
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
authors.forename: Xinqiong
authors.initials: X
authors.lastname: Liu
keywords: field resistance; rice blast disease; signalling pathway; true resistance