title: Differential Expression of microRNAs in the Ovaries from Letrozole-Induced Rat Model of Polycystic Ovary Syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder. To understand the pathogenesis of PCOS, we established rat models of PCOS induced by letrozole and employed deep sequencing to screen the differential expression of microRNAs (miRNAs) in PCOS rats and control rats. We observed vaginal smear and detected ovarian pathological alteration and hormone level changes in PCOS rats. Deep sequencing showed that a total of 129 miRNAs were differentially expressed in the ovaries from letrozole-induced rat model compared with the control, including 49 miRNAs upregulated and 80 miRNAs downregulated. Furthermore, the differential expression of miR-201-5p, miR-34b-5p, miR-141-3p, and miR-200a-3p were confirmed by real-time polymerase chain reaction. Bioinformatic analysis revealed that these four miRNAs were predicted to target a large set of genes with different functions. Pathway analysis supported that the miRNAs regulate oocyte meiosis, mitogen-activated protein kinase (MAPK) signaling, phosphoinositide 3-kinase/Akt (PI3K-Akt) signaling, Rap1 signaling, and Notch signaling. These data indicate that miRNAs are differentially expressed in rat PCOS model and the differentially expressed miRNA are involved in the etiology and pathophysiology of PCOS. Our findings will help identify miRNAs as novel diagnostic markers and therapeutic targets for PCOS.
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .|2 Department of Gynecological Oncology, Tumor Hospital of Jilin Province , Changchun, People's Republic of China .
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: 3 College of Animal Sciences, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun, People's Republic of China .
authors.forename: Chunjin
authors.initials: C
authors.lastname: Li
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 4 Department of Breast Oncology, Tumor Hospital of Jilin Province , Changchun, People's Republic of China .
authors.forename: Duo
authors.initials: D
authors.lastname: Xu
authors.affiliation: 3 College of Animal Sciences, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun, People's Republic of China .
authors.forename: Hongjiao
authors.initials: H
authors.lastname: Li
authors.affiliation: 5 Department of Gynecology and Obstetrics, Zhengzhou Central Hospital Affiliated to Zhengzhou University , Zhengzhou, People's Republic of China .
authors.forename: Liwei
authors.initials: L
authors.lastname: Gao
authors.affiliation: 3 College of Animal Sciences, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun, People's Republic of China .
authors.forename: Shuxiong
authors.initials: S
authors.lastname: Chen
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Lulu
authors.initials: L
authors.lastname: Fu
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Xueying
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Jingshun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Hao
authors.initials: H
authors.lastname: Ming
authors.affiliation: 1 Reproductive Medical Center, The Second Hospital of Jilin University , Changchun, People's Republic of China .
authors.forename: Lianwen
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D000077289:Letrozole; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009570:Nitriles; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced / Q000378:metabolism*; D034622:RNA Interference; D017208:Rats, Wistar; D014230:Triazoles
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D009570:Nitriles; D014230:Triazoles; D000077289:Letrozole