title: Therapeutic Role of Resveratrol and Quercetin on Aortic Fibroblasts of Psammomys obesus After Oxidative Stress by Hydrogen Peroxide.
abstract: In our study, we propose to analyze the effects of resveratrol (RES) and quercetin (QRC) on proliferation markers, oxidative stress, apoptosis, and inflammation of aortic fibroblasts of Psammomys obesus after induced oxidative stress by hydrogen peroxide (H2O2). Fibroblasts were incubated in RES 375 μM and QRC 0.083 μM for 24 hours after exposure to H2O2 1.2 mM for 6 hours. We performed the proliferation rate, cells viability, morphological analyses, cytochrome c, Akt, ERK1/2, and p38 MAPK quantification. The redox status was achieved by proportioning of malondialdehyde, nitric monoxide, advanced oxidation protein products, carbonyl proteins, catalase, and superoxide dismutase activity. The inflammation was measured by TNFα, MCP1, and NF-kB assay. The extracellular matrix (ECM) remodeling was performed by SDS-PAGE. Stressed fibroblasts showed a decrease of cell proliferation and viability, hypertrophy and oncosis, chromatin hypercondensation and increase of cytochrome c release characteristic of apoptosis, activation of ERK1/2 and Akt pathway, and decreases in p38 MAPK pathways marking the cellular resistance. The redox state was disrupted by increased malondialdehyde, nitric monoxide, advanced oxidation protein products, carbonyl protein production, catalase and superoxide dismutase activity, and a decreased production of proteins including collagens. Inflammation state was marked by MCP-1, TNFα, and NF-kB increase. Treatment of fibroblasts stressed by RES and QRC inverted the oxidative stress situation decreasing apoptosis and inflammation, and improving the altered redox status and rearrangement of disorders observed in extracellular matrix. H2O2 induced biochemical and morphological alterations leading to apoptosis. An improved general condition is observed after treatment with RES and QRC; this explains the antioxidant and antiapoptotic effects of polyphenols.
authors.affiliation: Laboratory of Physiology and Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Saliha
authors.initials: S
authors.lastname: Boumaza
authors.affiliation: Plant Physiology Laboratory, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Aicha
authors.initials: A
authors.lastname: Belkebir
authors.affiliation: Laboratory of Physiology and Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Samia
authors.initials: S
authors.lastname: Neggazi
authors.affiliation: Laboratory of Physiology and Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Hamid
authors.initials: H
authors.lastname: Sahraoui
authors.affiliation: Laboratory of Physiology and Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Sihem
authors.initials: S
authors.lastname: Berdja
authors.affiliation: Laboratory of Physiology and Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Leila
authors.initials: L
authors.lastname: Smail
authors.affiliation: Laboratory of Biochemistry and Remodeling Extra Cellular Matrix, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Benazzoug
authors.affiliation: Laboratory of Biochemistry, Central Hospital of the Army, Algiers, Algeria.
authors.forename: Ghoti
authors.initials: G
authors.lastname: Kacimi
authors.affiliation: Laboratory of Physiology and Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, Algiers, Algeria.
authors.forename: Souhila
authors.initials: S
authors.lastname: Aouichat Bouguerra
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta / Q000166:cytology; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005849:Gerbillinae; D006861:Hydrogen Peroxide / Q000633:toxicity; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D013267:Stilbenes; D011794:Quercetin; D006861:Hydrogen Peroxide; D000077185:Resveratrol