title: A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.
abstract: Libraries of transgenic Drosophila melanogaster carrying RNA interference (RNAi) constructs have been used extensively to perform large-scale functional genetic screens in vivo. For example, RNAi screens have facilitated the discovery of multiple components of the Hippo pathway, an evolutionarily conserved growth-regulatory network. Here we investigate an important technical limitation with the widely used VDRC KK RNAi collection. We find that approximately 25% of VDRC KK RNAi lines cause false-positive enhancement of the Hippo pathway, owing to ectopic expression of the Tiptop transcription factor. Of relevance to the broader Drosophila community, ectopic tiptop (tio) expression can also cause organ malformations and mask phenotypes such as organ overgrowth. To enhance the use of the VDRC KK RNAi library, we have generated a D. melanogaster strain that will allow researchers to test, in a single cross, whether their genetic screen of interest will be affected by ectopic tio expression.
authors.affiliation: Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre, 7 St Andrews Place, East Melbourne, Melbourne, Victoria 3002, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Joseph H A
authors.initials: JH
authors.lastname: Vissers
authors.affiliation: Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre, 7 St Andrews Place, East Melbourne, Melbourne, Victoria 3002, Australia.|Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Manning
authors.affiliation: Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre, 7 St Andrews Place, East Melbourne, Melbourne, Victoria 3002, Australia.
authors.forename: Aishwarya
authors.initials: A
authors.lastname: Kulkarni
authors.affiliation: Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre, 7 St Andrews Place, East Melbourne, Melbourne, Victoria 3002, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.|Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Kieran F
authors.initials: KF
authors.lastname: Harvey
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000066630:Ectopic Gene Expression; D005260:Female; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D034622:RNA Interference; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D000091102:YAP-Signaling Proteins; D000099317:Hippo Kinases
chemical_list: D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D015534:Trans-Activators; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C503814:Yki protein, Drosophila; C496672:tio protein, Drosophila; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases