title: Paenibacillus bovissp. nov., isolated from raw yak (Bos grunniens) milk.
abstract: Strain BD3526T, isolated from raw yak milk collected in Tibet, China, was studied to determine its taxonomic status. The strain was Gram-reaction positive, motile, catalase-positive, aerobic or facultatively anaerobic. The DNA G+C content of the strain was 47.5 mol% and its peptidoglycan containedmeso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0and C16 : 0. Based on 16S rRNA gene sequence analysis, similarities among strain BD3526Tand its most closely related species,Paenibacillus shenyangensisA9T, 'Paenibacillus dauci' H9, 'Paenibacillus wulumuqiensis' Y24 andPaenibacillus hunanensisDSM 22170Twere 99.0, 98.5, 97.3 and 96.7 %, respectively. Levels of DNA-DNA relatedness among strain BD3526TandP. hunanensisDSM 22170TandPaenibacillus polymyxaATCC 842T( = DSM 36T), the type species of the genus, were 41.2 and 45.6 %, respectively.In silicogenome-to-genome comparison showed that the DNA-DNA hybridization values among strain BD3526TandP. shenyangensisA9T, 'P. dauci' H9 and 'P. wulumuqiensis' Y24 were lower than 70 %. Based on its molecular and physiological properties, strain BD3526T( = DSM 28815T= CGMCC 8333T) is identified as the type strain of a novel species within the genusPaenibacillus, for which the namePaenibacillus bovisis proposed.
authors.affiliation: State Key Laboratory of Dairy Biotechnology,Shanghai 200436,PR China.|Shanghai Engineering Research Center of Dairy Biotechnology,Shanghai 200436,PR China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Dairy Biotechnology,Shanghai 200436,PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: State Key Laboratory of Dairy Biotechnology,Shanghai 200436,PR China.|Dairy Research Institute, Bright Dairy & Food Co., Ltd,Shanghai 200436,PR China.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shanghai Engineering Research Center of Dairy Biotechnology,Shanghai 200436,PR China.|Dairy Research Institute, Bright Dairy & Food Co., Ltd,Shanghai 200436,PR China.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Xu
authors.affiliation: Shanghai Engineering Research Center of Dairy Biotechnology,Shanghai 200436,PR China.|Dairy Research Institute, Bright Dairy & Food Co., Ltd,Shanghai 200436,PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hang
authors.affiliation: State Key Laboratory of Dairy Biotechnology,Shanghai 200436,PR China.|Shanghai Engineering Research Center of Dairy Biotechnology,Shanghai 200436,PR China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Wu