title: Antibiotic-treated versus germ-free rodents for microbiota transplantation studies.
abstract: We recently investigated the applicability of antibiotic-treated recipient mice for transfer of different gut microbiota profiles. With this addendum we elaborate on perspectives and limitations of using antibiotics as an alternative to germ-free (GF) technology in microbial transplantation studies, and we speculate on the housing effect. It is possible to transfer host phenotypes via fecal transplantation to antibiotic-treated animals, but problems with reproducibility, baseline values, and antibiotic resistance genes should be considered. GF animals maintained in isolators still seem to be the best controlled models for long-term microbial transplantation, but antibiotic-treated recipients are also commonly utilized. We identify a need for systematic experiments investigating the stability of microbial transplantations by addressing 1) the recipient status as either GF, antibiotic-treated or specific pathogen free and 2) different levels of protected housing systems. In addition, the developmental effect of microbes on host physiological functions should be evaluated in the different scenarios.
authors.affiliation: a Section of Experimental Animal Models , Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg C , Denmark.|b Internal Research and Development, Taconic Biosciences , Lille Skensved , Denmark.
authors.forename: Randi
authors.initials: R
authors.lastname: Lundberg
authors.affiliation: b Internal Research and Development, Taconic Biosciences , Lille Skensved , Denmark.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Toft
authors.affiliation: b Internal Research and Development, Taconic Biosciences , Lille Skensved , Denmark.
authors.forename: Benjamin
authors.initials: B
authors.lastname: August
authors.affiliation: a Section of Experimental Animal Models , Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg C , Denmark.
authors.forename: Axel K
authors.initials: AK
authors.lastname: Hansen
authors.affiliation: a Section of Experimental Animal Models , Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg C , Denmark.
authors.forename: Camilla H F
authors.initials: CH
authors.lastname: Hansen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000069467:Fecal Microbiota Transplantation / Q000379:methods*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D051379:Mice; D023421:Models, Animal; D015203:Reproducibility of Results; D013047:Specific Pathogen-Free Organisms
chemical_list: D000900:Anti-Bacterial Agents
keywords: animal models; antibiotics; fecal transplantation; germ-free; gut microbiota; mouse; reproducibility