title: Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm.
abstract: OBJECTIVE
  To check the antimicrobial activity of Azadirachta indica (Neem), Mimusops elengi (Bakul), and Chlorhexidine gluconate (CHX) on multispecies biofilm of common endodontic pathogens such as Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans.
  
  
  METHODS
  In vitro dentin disinfection model used to check the antimicrobial efficacy of herbal extracts.
  
  
  METHODS
  The in vitro dentin disinfection model was used to check the antimicrobial activity of the methanolic extracts of the medicinal plants along with Chlorhexidine gluconate. The polymicrobial biofilm was allowed to grow on extracted teeth sections for a period of 21 days. Remaining microbial load in the form of CFU/ml after the antimicrobial treatment was tabulated, and data were statistically analyzed using ANOVA and Bonferroni post-hoc tests.
  
  
  METHODS
  SPSS version 17, one-way ANOVA, Bonferroni post-hoc test.
  
  
  RESULTS
  Both the plant extracts showed considerable antimicrobial efficacy as compared to negative control. 2% CHX was the most effective antimicrobial agent having statistically significant difference against plant extracts and negative control (saline).
  
  
  CONCLUSIONS
  The methanolic extract of A. Indica, M. elengi, and Chlorhexidine Gluconate has considerable antimicrobial activity against polymicrobial dentinal biofilm of S. mutans, E. faecalis, S. aureus and C. albicans.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Faculty of Dental Science, Dharamsinh Desai University, Nadiad, Gujarat, India.
authors.forename: Kunjal S
authors.initials: KS
authors.lastname: Mistry
authors.affiliation: Department of Conservative Dentistry and Endodontics, Ahmedabad Dental College and Hospital, Ahmedabad, Gujarat, India.
authors.forename: Zarna
authors.initials: Z
authors.lastname: Sanghvi
authors.affiliation: Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Ahmedabad, Gujarat, India.
authors.forename: Girish
authors.initials: G
authors.lastname: Parmar
authors.affiliation: Department of Pharmacology, Sardar Patel College of Pharmacy, Anand, Gujarat, India.
authors.forename: Samir
authors.initials: S
authors.lastname: Shah
authors.affiliation: Senior Research Associate, Disha Life Science, Ahmedabad, Gujarat, India.
authors.forename: Kasukurthi
authors.initials: K
authors.lastname: Pushpalatha
keywords: Antimicrobial activity; Chlorhexidine gluconate; medicinal plant extracts; multispecies dentinal biofilm