title: Modular Design of Poly(norbornenes) for Organelle-Specific Imaging in Tumor Cells.
abstract: Through modular ROMP (ring-opening metathesis polymerization) directly from monomeric norbornenes of bioactive peptides, rhodamine B chromophore, and PEG solubilizer, we designed and synthesized a series of water-soluble poly(norbornenes) with organelle-specific imaging capability in tumor cells. For the selection of FxrFxK, TAT, and SV40 peptide sequences, these fluorescence probes exhibited different targeting specificity toward mitochondria, lysosome, and nucleolus, respectively, based on the same poly(norbornene) backbonds. More importantly, the ROMP strategy enables selective combination from various monomers and allows programmable biofunctionalization via peptide sequence permutations, which would greatly extend the biomedical applications such as imaging, diagnosis, and therapy for these synthetic polymers.
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, The Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Feng
authors.affiliation: Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, Beijing Laboratory of Biomedical Materials, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University , Beijing 100069, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xie
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, The Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, The Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Chen-Ho
authors.initials: CH
authors.lastname: Tung
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, The Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Li-Zhu
authors.initials: LZ
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D018613:Microscopy, Confocal; D009636:Norbornanes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D015388:Organelles / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D012235:Rhodamines / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009636:Norbornanes; D012235:Rhodamines; D011092:Polyethylene Glycols; C029773:rhodamine B