title: Elevated PIM2 gene expression is associated with poor survival of patients with acute myeloid leukemia.
abstract: The PIM2 gene encodes the serine/threonine kinase involved in cell survival and apoptosis. The aim of the study was to evaluate the expression of the PIM2 gene in acute myeloid leukemia (AML) and to examine its role in apoptosis of the blastic cells. We analyzed the PIM2 expression in 148 patients: 91 with AML, 57 with acute lymphoblastic leukemia and 24 healthy controls by Real-Time PCR and Western blot. Inhibition of the PIM2 gene in human leukemic HL60 cell line was performed with RNAi and apoptosis rate was analyzed. Our results indicate that overexpression of PIM2 in AML is associated with low complete remission rate, high-risk cytogenetics, shorter leukemia-free survival, and event-free survival. Cytometric analysis of HL60/PAC-GFP and HL60/PAC-GFP-shPIM2 cells revealed an increase in the number of apoptotic cells after inhibition of PIM2 gene. In summary, the elevated expression of PIM2 in blastic cells is associated with poor prognosis of AML patients and their resistance to induction therapy.
authors.affiliation: a Department of Hematology, Neoplastic Blood Disorders and Bone Marrow Transplantation , Wroclaw Medical University , Wroclaw , Poland ;
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kapelko-Slowik
authors.affiliation: b Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences , Wroclaw , Poland ;|c Department of Biochemistry, Pharmacology and Toxicology , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland ;
authors.forename: Tomasz B
authors.initials: TB
authors.lastname: Owczarek
authors.affiliation: c Department of Biochemistry, Pharmacology and Toxicology , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland ;
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Grzymajlo
authors.affiliation: a Department of Hematology, Neoplastic Blood Disorders and Bone Marrow Transplantation , Wroclaw Medical University , Wroclaw , Poland ;
authors.forename: Donata
authors.initials: D
authors.lastname: Urbaniak-Kujda
authors.affiliation: a Department of Hematology, Neoplastic Blood Disorders and Bone Marrow Transplantation , Wroclaw Medical University , Wroclaw , Poland ;
authors.forename: Bozena
authors.initials: B
authors.lastname: Jazwiec
authors.affiliation: d Department of Ophthalmology , Wroclaw Medical University , Wroclaw , Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Slowik
authors.affiliation: a Department of Hematology, Neoplastic Blood Disorders and Bone Marrow Transplantation , Wroclaw Medical University , Wroclaw , Poland ;
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kuliczkowski
authors.affiliation: b Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences , Wroclaw , Poland ;|c Department of Biochemistry, Pharmacology and Toxicology , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland ;
authors.forename: Maciej
authors.initials: M
authors.lastname: Ugorski
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012074:Remission Induction; D055815:Young Adult; D051025:bcl-Associated Death Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C494819:BAD protein, human; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C087000:EIF4EBP1 protein, human; C490587:PIM2 protein, human; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D051025:bcl-Associated Death Protein; D017346:Protein Serine-Threonine Kinases
keywords: 4E-BP1; BAD; PIM2; acute myeloid leukemia; apoptosis