title: Identification and characterisation of a biosynthetic locus for Moraxella bovis lipo-oligosaccharide.
abstract: Moraxella bovis is a Gram-negative gammaproteobacterium and is one of the causative agents of infectious bovine keratoconjunctivitis. The structure of lipooligosaccharide (LOS) from strain Epp63 was recently elucidated. In the present study a genetic locus of seven encoding genes with high similarity to glycosyltransferases has been identified. Mutation of these putative glycosyltransferase genes resulted in M. bovis mutant bacteria that expressed truncated LOS structures. The structures of the oligosaccharide (OS) expressed by the mutant strains were elucidated and demonstrated the role of the glycosyltransferase enzymes in the LOS biosynthesis of M. bovis. The glycosyltransferase genes designated lgt1, lgt3, and lgt6 are highly similar to the genes in the related bacterium M. catarrhalis. In addition, there are syntenic similarities with the corresponding LOS biosynthesis locus in M. catarrhalis and other members of Moraxellaceae.
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Faglin
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia; School of Medical Science, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia.
authors.forename: I Darren
authors.initials: ID
authors.lastname: Grice
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia.
authors.forename: S R A M Eranda
authors.initials: SRAME
authors.lastname: Ratnayake
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia.
authors.forename: Terese-Marie
authors.initials: TM
authors.lastname: Daal
authors.affiliation: School of Medical Science, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia.
authors.forename: Sanjesh
authors.initials: S
authors.lastname: Singh
authors.affiliation: School of Medical Science, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia; Menzies Health Institute, Molecular Basis of Disease, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia. Electronic address: jennifer.wilson@griffith.edu.au.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Wilson
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia; School of Medical Science, Griffith University, Gold Coast Campus, Southport, Qld. 4222, Australia. Electronic address: i.peak@griffith.edu.au.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Peak
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D003198:Computer Simulation; D056426:Genetic Loci; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D016955:Moraxella bovis / Q000235:genetics*; D009154:Mutation; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D016695:Glycosyltransferases
keywords: Glycosyltransferase; Infectious bovine keratoconjunctivitis; Lipo-oligosaccharide; Moraxella bovis