title: Molecular characterization of novel immunodominant molybdenum cofactor biosynthesis protein C1 (Rv3111) from Mycobacterium tuberculosis H37Rv.
abstract: BACKGROUND
  In the molybdenum cofactor biosynthesis pathway, MoaA and MoaC catalyze the first step of transformation of GTP to cPMP. In M. tuberculosis H37Rv, three different genes (Rv3111, Rv0864 and Rv3324c) encode for MoaC homologs. Out of these three only MoaC1 (Rv3111) is secretory in nature.
  
  
  METHODS
  We have characterized MoaC1 protein through biophysical, in-silico, and immunological techniques.
  
  
  RESULTS
  We have characterized the conformation and thermodynamic stability of MoaC1, and have established its secretory nature by demonstrating the presence of anti-MoaC1 antibodies in human tuberculosis patients' sera. Further, MoaC1 elicited a dominant Th1 immune response in mice characterized by increased induction of IL-2 and IFN-γ.
  
  
  CONCLUSIONS
  Integrating these results, we conclude that MoaC1 is a structured secretory protein capable of binding with GTP and eliciting induced immune response.
  
  
  CONCLUSIONS
  This study would be useful for the development of vaccines against tuberculosis and to improve methods used for diagnosis of tuberculosis.
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
authors.forename: Shubhra
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Pathak
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Pandey
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
authors.forename: Sarita
authors.initials: S
authors.lastname: Tripathi
authors.affiliation: King George Medical University, Lucknow, Uttar Pradesh 226003, India.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Garg
authors.affiliation: Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
authors.forename: Shailja
authors.initials: S
authors.lastname: Misra-Bhattacharya
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India. Electronic address: ashishcdri@yahoo.com.
authors.forename: Ashish
authors.initials: A
authors.lastname: Arora
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D008297:Male; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D055550:Protein Stability; D017386:Sequence Homology, Amino Acid; D018417:Th1 Cells / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D007376:Interleukin-2; D032581:Tuberculosis Vaccines; D007371:Interferon-gamma
keywords: MoCo biosynthesis; MoaC1; Mycobacterium tuberculosis; Rv3111; Th1/Th2 cytokine