title: Sqt1p is an eight-bladed WD40 protein.
abstract: Ribosome biogenesis in eukaryotes is a complex and highly orchestrated process involving more than 200 accessory factors in addition to ribosomal RNAs and ribosomal proteins. Among the many factors involved, Sqt1p has been reported to specifically bind to uL16 and to act as a chaperone. The crystal structure of full-length Sqt1p from the yeast Saccharomyces cerevisiae has been solved at 3.35 Å resolution. A SAD experiment at the Se K edge and an S-SAD experiment on the same selenomethionine-substituted protein crystal allowed unambiguous positioning of the selenomethionine and Cys residues. On the basis of the atomic structure of Sqt1p, the potential residues involved in uL16 interaction were identified and tested.
authors.affiliation: Université de Bordeaux, IECB, 33607 Pessac, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Frénois
authors.affiliation: Experimental Division, Synchrotron SOLEIL, BP 48, L'Orme des Merisiers, 91192 Gif-sur-Yvette, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Legrand
authors.affiliation: Université de Bordeaux, IECB, 33607 Pessac, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fribourg
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D012269:Ribosomal Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D012269:Ribosomal Proteins; C107942:SQT1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: 60S; Sqt1; WD repeat; ribosome biogenesis; uL16