title: Anti-Melanogenic Potentials of Nanoparticles from Calli of Resveratrol-Enriched Rice against UVB-Induced Hyperpigmentation in Guinea Pig Skin.
abstract: We already reported that genetically engineered resveratrol-enriched rice (RR) showed to down-regulate skin melanogenesis. To be developed to increase the bioactivity of RR using calli from plants, RR was adopted for mass production using plant tissue culture technologies. In addition, high-pressure homogenization (HPH) was used to increase the biocompatibility and penetration of the calli from RR into the skin. We aimed to develop anti-melanogenic agents incorporating calli of RR (cRR) and nanoparticles by high-pressure homogenization, examining the synergistic effects on the inhibition of UVB-induced hyperpigmentation. Depigmentation was observed following topical application of micro-cRR, nano-calli of normal rice (cNR), and nano-cRR to ultraviolet B (UVB)-stimulated hyperpigmented guinea pig dorsal skin. Colorimetric analysis, tyrosinase immunostaining, and Fontana-Masson staining for UVB-promoted melanin were performed. Nano-cRR inhibited changes in the melanin color index caused by UVB-promoted hyperpigmentation, and demonstrated stronger anti-melanogenic potential than micro-cRR. In epidermal skin, nano-cRR repressed UVB-promoted melanin granules, thereby suppressing hyperpigmentation. The UVB-enhanced, highly expressed tyrosinase in the basal layer of the epidermis was inhibited by nano-cRR more prominently than by micro-cRR and nano-cNR. The anti-melanogenic potency of nano-cRR also depended on pH and particle size. Nano-cRR shows promising potential to regulate skin pigmentation following UVB exposure.
authors.affiliation: College of Pharmacy, Yonsei University, Incheon 21983, Republic of Korea.
authors.forename: Taek Hwan
authors.initials: TH
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea.
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Kang
authors.affiliation: College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea.
authors.forename: Jae Ok
authors.initials: JO
authors.lastname: Seo
authors.affiliation: National Institute of Crop Science, Rural Development Administration, Iksan 54663, Republic of Korea.
authors.forename: So-Hyeon
authors.initials: SH
authors.lastname: Baek
authors.affiliation: Anti-aging Research Institute, BIO-FD&C Co., LTD, Incheon 21990, Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Moh
authors.affiliation: College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea.
authors.forename: Jae Kyoung
authors.initials: JK
authors.lastname: Chae
authors.affiliation: College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea.
authors.forename: Yong Un
authors.initials: YU
authors.lastname: Park
authors.affiliation: College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea.
authors.forename: Young Tag
authors.initials: YT
authors.lastname: Ko
authors.affiliation: College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea; Gachon Medical Research Institute, Gil Medical Center, Inchon 21565, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 21936, Republic of Korea.
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
keywords: High-pressure homogenization; Melanogenesis; Nanoparticles; Plant tissue culture; Resveratrol-enriched rice