title: Respiratory Toxicity of Dimethyl Sulfoxide.
abstract: Dimethyl sulfoxide (DMSO) is one of the most commonly used solvents for hydrophobic substances in biological experiments. In addition, the compound exhibits a plethora of bioactivities, which makes it of potential pharmacological use of its own. The influence on respiration, and thus on arterial blood oxygenation, of DMSO is unclear, contentious, and an area of limited study. Thus, in the present investigation we set out to determine the influence on lung ventilation of cumulated doses of DMSO in the amount of 0.5, 1.5, 3.5, 7.5, and 15.5 g/kg; each dose given intraperitoneally at 1 h interval in conscious mice. Ventilation and its responses to 7 % hypoxia (N(2) balanced) were recorded in a whole body plethsymograph. We demonstrate a dose-dependent inhibitory effect of DMSO on lung ventilation and its hypoxic responsiveness, driven mostly by changes in the tidal component. The maximum safe dose of DMSO devoid of meaningful consequences for respiratory function was 3.5 g/kg. The dose of 7.5 g/kg of DMSO significantly dampened respiration, with yet well preserved hyperventilatory response to hypoxia. The highest dose of 15.5 g/kg severely impaired ventilation and its responses. The study delineates the safety profile of DMSO regarding the respiratory function which is essential for maintaining proper tissue oxygenation. Caution should be exercised concerning dose concentration of DMSO.
authors.affiliation: Clinical Research Center, National Hospital Organization Murayama Medical Center, 2-37-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.|Fujita Memorial Nanakuri Institute, Fujita Health University, 423 Oodori-cho, Tsu, 514-1296, Mie, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Clinical Research Center, National Hospital Organization Murayama Medical Center, 2-37-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan. m_pokorski@hotmail.com.|Public Higher Medical Professional School in Opole, 68 Katowicka St., 45-060, Opole, Poland. m_pokorski@hotmail.com.
authors.forename: Mieczyslaw
authors.initials: M
authors.lastname: Pokorski
authors.affiliation: Institute of Socio-Arts and Sciences, The University of Tokushima, 1-1 Minamijosanjima-cho, 770-8502, Tokushima, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Respiratory Medicine, National Hospital Organization Tokyo Medical Center, 2-5-1 Higashigaoka, Meguro, 152-8902, Tokyo, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Oyamada
authors.affiliation: Clinical Research Center, National Hospital Organization Murayama Medical Center, 2-37-1 Gakuen, Musashimurayama, 208-0011, Tokyo, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000818:Animals; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012119:Respiration / Q000187:drug effects*
chemical_list: D004121:Dimethyl Sulfoxide
keywords: Dimethyl sulfoxide; Hypoxia; Mice; Neural function; Respiration; Solvent; Toxicity