title: [Chemical Constituents from Leaves of Hibiscus syriacus and Their α-Glucosidase Inhibitory Activities].
abstract: OBJECTIVE
  To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities.
  
  
  METHODS
  Column chromatography including macroporous resins, silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties, 1H-NMR and 13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate.
  
  
  RESULTS
  15 compounds were isolated and identified as β-sitosterol(1), β-daucostero (2), β-amyrin (3), oleanolic acid (4), stigmast-4-en-3-one (5), friedelin (6), syriacusin A (7), kaempferol (8), isovitexin (9), vitexin (10), apigenin (11), apigenin-7-O-β-D-glucopyranoside (12), luteolin-7-O-β-D-glucopyranoside (13), vitexin-7-O-β-D-glucopyranoside (14) and rutin (15).
  
  
  CONCLUSIONS
  All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control, the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39.03 ± 0.38 and 32.12 ± 0.62 mg/L, inhibition ratio of 94.95% and 97.15%, respectively.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Ji
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Qian-rong
authors.initials: QR
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
mesh_terms: D047310:Apigenin; D005960:Glucosides; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry*; D031584:Hibiscus / Q000737:chemistry*; D044949:Kaempferols; D047311:Luteolin; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D064209:Phytochemicals / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D012855:Sitosterols; D013265:Stigmasterol / Q000031:analogs & derivatives; D014315:Triterpenes; D000520:alpha-Glucosidases; D000094042:Lupanes
chemical_list: D047310:Apigenin; D005960:Glucosides; D065089:Glycoside Hydrolase Inhibitors; D044949:Kaempferols; D047311:Luteolin; D009828:Oleanolic Acid; D064209:Phytochemicals; D012855:Sitosterols; D013265:Stigmasterol; D014315:Triterpenes; D000520:alpha-Glucosidases; C488470:stigmast-4-en-3-one; C025473:gamma-sitosterol; C006552:kaempferol; C057792:apigetrin; C066408:luteolin-7-glucoside; C032731:vitexin; C060796:friedelin; D000094042:Lupanes; C036380:beta-amyrin; C049772:isovitexin