title: Acute-phase glycoprotein N-glycome of ovarian cancer patients analyzed by CE-LIF.
abstract: Epithelial ovarian cancer (EOC) is the most frequent cause of death from all gynecological malignancies because of its late diagnosis. As N-glycosylation is modified in the course of ovarian cancer, it is a promising source of tumor biomarkers. In this work, serum glycoproteins, depleted from albumin and IgG, were separated by 2DE. Protein spots of acute-phase proteins were identified by peptide mapping and their corresponding glycan moieties were released enzymatically, fluorescently labeled and analyzed by CE-LIF. In the positive acute-phase proteins, haptoglobin, α1-antitrypsin, and α1-antichymotrypsin, an increase of antennarity and Lewis(X) motif could be measured in EOC patients on tri- and/or tetraantennary N-glycans. Tetraantennary N-glycans containing three Lewis(X) epitopes and triantennary N-glycans containing a β(1-6) branch and a Lewis(X) epitope were only present in EOC patients. We also showed for the first time that the core-fucosylated biantennary digalactosylated N-glycan of α1-acid glycoprotein is a potential biomarker for EOC. To conclude, core-fucosylated biantennary N-glycans on α1-acid glycoprotein as well as higher antennarity and increased amounts of Lewis(X) motif on haptoglobin, α1-antitrypsin, and α1-antichymotrypsin are promising biomarkers for EOC. Nevertheless, their specificity and selectivity for the early detection of EOC should be evaluated in a larger study.
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Medical University, Berlin, Germany.|Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Berlin, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Weiz
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Medical University, Berlin, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Wieczorek
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Medical University, Berlin, Germany.|Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schwedler
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Medical University, Berlin, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kaup
authors.affiliation: Department of Gynecology, Charité Medical University, Berlin, Germany.
authors.forename: Elena Iona
authors.initials: EI
authors.lastname: Braicu
authors.affiliation: Department of Gynecology, Charité Medical University, Berlin, Germany.
authors.forename: Jalid
authors.initials: J
authors.lastname: Sehouli
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Medical University, Berlin, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Tauber
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Medical University, Berlin, Germany.
authors.forename: Véronique
authors.initials: V
authors.lastname: Blanchard
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000097:blood; D000077216:Carcinoma, Ovarian Epithelial; D019075:Electrophoresis, Capillary; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D054794:Glycomics / Q000379:methods*; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry; D010051:Ovarian Neoplasms / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry
chemical_list: D000209:Acute-Phase Proteins; D014408:Biomarkers, Tumor; D011134:Polysaccharides
keywords: Acute-phase protein; CE-LIF; N-glycan; Ovarian cancer