title: High MicroRNA-370 Expression Correlates with Tumor Progression and Poor Prognosis in Breast Cancer.
abstract: OBJECTIVE
  Deregulation of microRNA-370 (miR-370) has been reported in various cancers, in which it can act as either an oncogene or a tumor suppressor gene. However, the clinicopathologic significance of miR-370 expression in breast cancer has not been studied.
  
  
  METHODS
  The expression of miR-370 was determined with quantitative real-time polymerase chain reaction in 60 formalin-fixed, paraffin-embedded primary breast cancer tissues. Additionally, the protein expression levels of previously known targets of miR-370, such as FOXM1, FOXO1, and FOXO3a, were detected using immunohistochemistry. Finally, we analyzed its correlation with target protein expression, clinicopathologic features, and clinical outcome.
  
  
  RESULTS
  High levels of miR-370 expression correlated with lymph node metastasis (p=0.009), advanced stage (p=0.002), and frequent perineural invasion (p=0.042). Moreover, patients with high miR-370 expression had poor disease-free survival compared with the low-expression group. However, no correlation was observed between miR-370 and its target protein expression.
  
  
  CONCLUSIONS
  Our results indicate that upregulation of miR-370 in breast cancer is correlated with breast cancer progression and that it might be a potential biomarker for predicting clinical outcomes.
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Sim
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Hyein
authors.initials: H
authors.lastname: Ahn
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Rehman
authors.initials: R
authors.lastname: Abdul
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Hyunsung
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Ki-Jong
authors.initials: KJ
authors.lastname: Yi
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Chung
authors.affiliation: Department of Surgery, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Chung
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Seung Sam
authors.initials: SS
authors.lastname: Paik
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Song
authors.affiliation: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Kiseok
authors.initials: K
authors.lastname: Jang
keywords: Breast neoplasms; MicroRNA-370; Prognosis; Real-time polymerase chain reaction