title: Bioanalytical LC-MS Method for the Quantification of Plasma Androgens and Androgen Glucuronides in Breast Cancer.
abstract: The physiological and pathological development of the breast is strongly affected by the hormonal milieu consisting of steroid hormones. Mass spectrometry (MS) technologies of high sensitivity and specificity enable the quantification of androgens and consequently the characterization of the hormonal status. The aim of this study is the assessment of plasma androgens and androgen glucuronides, in the par excellence hormone-sensitive tissue of the breast, through the application of liquid chromatography-mass spectrometry (LC-MS). A simple and efficient fit-for-purpose method for the simultaneous identification and quantification of dehydroepiandrosterone sulfate (DHEAS), androstenedione (A4), androsterone glucuronide (ADTG) and androstane-3α, 17β-diol-17-glucuronide (3α-diol-17G) in human plasma was developed and validated. The presented method permits omission of derivatization, requires a single solid-phase extraction procedure and the chromatographic separation can be achieved on a single C18 analytical column, for all four analytes. The validated method was successfully applied for the analysis of 191 human plasma samples from postmenopausal women with benign breast disease (BBD), lobular neoplasia (LN), ductal carcinoma in situ and invasive ductal carcinoma (IDC). DHEAS plasma levels exhibited significant differences between LN, IDC and BBD patients (P < 0.05). Additionally, ADTG levels were significantly higher in patients with LN compared with those with BBD (P < 0.05).
authors.affiliation: Research Center, Hellenic Anticancer Institute, 11 Valtetsiou st, Athens, 10680, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Kalogera
authors.affiliation: Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Pistos
authors.affiliation: Research Center, Hellenic Anticancer Institute, 11 Valtetsiou st, Athens, 10680, Greece.
authors.forename: Xeni
authors.initials: X
authors.lastname: Provatopoulou
authors.affiliation: Cyprus International Institute for Environmental and Public Health in association with Harvard School of Public Health, Cyprus University of Technology, Limassol, Cyprus Department of Environmental Health, Harvard School of Public Health, Boston, MA, USA.
authors.forename: Costas A
authors.initials: CA
authors.lastname: Christophi
authors.affiliation: Breast Unit, 1st Department of Propaedeutic Surgery, Hippokratio Hospital, University of Athens, Athens, Greece.
authors.forename: George C
authors.initials: GC
authors.lastname: Zografos
authors.affiliation: Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Stefanidou
authors.affiliation: Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Chara
authors.initials: C
authors.lastname: Spiliopoulou
authors.affiliation: Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Sotirios
authors.initials: S
authors.lastname: Athanaselis
authors.affiliation: Research Center, Hellenic Anticancer Institute, 11 Valtetsiou st, Athens, 10680, Greece antgouna@otenet.gr.
authors.forename: Antonia
authors.initials: A
authors.lastname: Gounaris
mesh_terms: D000728:Androgens / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D020719:Glucuronides / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D000728:Androgens; D020719:Glucuronides