title: Using PG-Liposome-Based System to Enhance Puerarin Liver-Targeted Therapy for Alcohol-Induced Liver Disease.
abstract: A critical issue for alcohol-induced liver disease (ALD) therapeutics is the lack of a highly efficient delivery system. In this study, a Puerarin-propylene glycol-liposome system was prepared for the purpose of targeting puerarin, an isoflavon, to the liver. Transmission electron microscope (TEM) results showed the liposomes to be spherical in shape with an average diameter of 182 nm with a polydispersity index of 0.239. The zeta potential of the particles was about -30 mV. The entrapment efficiency of puerarin was above 90%. MTT-based assay in HpeG2 cells showed no significant cytotoxicity in the presence of up to 25% concentration of the system containing 3% puerarin. In vivo performance of this system was studied in mice. Pharmacokinetics and distribution of puerarin-PG-liposome system was studied relative to puerarin solution at the same dose levels. The results show that puerarin-PG-liposome prolonged drug retention time and decreased elimination of puerarin in mice (AUC of liposome system and solution was 9.5 and 4.0 mg h L-1, respectively). Furthermore, propylene glycol (PG)-liposome system enhanced puerarin distribution into liver and spleen, while decreasing puerarin distribution in other tissues. Overall, the puerarin-PG-liposome system showed enhanced therapeutic effect in mice with ALD.
authors.affiliation: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.|The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Ying-Zheng
authors.initials: YZ
authors.lastname: Zhao
authors.affiliation: The Third People's Hospital of Hangzhou, Hangzhou, Zhejiang, 310000, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Philadelphia College of Pharmacy, University of the Sciences in Philadelphia, Philadelphia, Pennsylvania, 19104, USA.
authors.forename: Pardeep K
authors.initials: PK
authors.lastname: Gupta
authors.affiliation: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Tian
authors.affiliation: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Mao
authors.affiliation: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Kai-Yan
authors.initials: KY
authors.lastname: Qiu
authors.affiliation: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Hainan Medical College, Haikou, Hainan, 570102, China.
authors.forename: Chuan-Zhu
authors.initials: CZ
authors.lastname: Lv
authors.affiliation: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. lctuua@yahoo.com.cn.|The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China. lctuua@yahoo.com.cn.
authors.forename: Cui-Tao
authors.initials: CT
authors.lastname: Lu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D016503:Drug Delivery Systems / Q000379:methods; D000431:Ethanol / Q000009:adverse effects; D056945:Hep G2 Cells; D006801:Humans; D007529:Isoflavones / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010316:Particle Size; D019946:Propylene Glycol / Q000737:chemistry*; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D007529:Isoflavones; D008081:Liposomes; D000431:Ethanol; D019946:Propylene Glycol; C033607:puerarin
keywords: alcohol-induced liver disease; distribution; pharmacokinetics; propylene glycol liposome; puerarin