title: NewPaenibacillus larvaebacterial isolates from honey bee colonies infected with American foulbrood disease in Egypt.
abstract: The American foulbrood disease is widely distributed all over the world and causes a serious problem for the honeybee industry. Different infected larvae were collected from different apiaries, ground in phosphate saline buffer (PSB) and bacterial isolation was carried out on nutrient agar medium. Different colonies were observed and were characterized biologically. Two bacterial isolates (SH11 and SH33) were subjected to molecular identification using 16S rRNA gene and the sequence analysis revealed that the two isolates arePaenibacillus larvaewith identity not exceeding 83%. The DNA sequence alignment between the otherP. larvaebacterial strains and the two identified bacterial isolates showed that all the examined bacterial strains have the same ancestor, i.e. they have the same origin. The SH33 isolate was closely related to theP. larvaeisolated from Germany, whereas the isolate SH11 was close to theP. larvaeisolated from India. The phylogenetic tree constructed for 20 differentBacillussp. and the two isolates SH11 and SH33 demonstrated that the two isolates areBacillussp. and they are new isolates. The bacterial isolates will be subjected to more tests for more confirmations.
authors.affiliation: Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications , Alexandria , Egypt.
authors.forename: Saad Hamdy Daif
authors.initials: SH
authors.lastname: Masry
authors.affiliation: Protein Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications , Alexandria , Egypt.
authors.forename: Sanaa Soliman
authors.initials: SS
authors.lastname: Kabeil
authors.affiliation: Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications , Alexandria , Egypt.
authors.forename: Elsayed Elsayed
authors.initials: EE
authors.lastname: Hafez
keywords: 16S rRNA; American foulbrood disease; Paenibacillus larvae; honey bee (Apis mellifera L.)