title: Structural characterization and biological activities of two α-glucans from radix paeoniae alba.
abstract: Radix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, cancer, and other diseases. In this study, two polysaccharides RPAPW1 and RPAPW2 were isolated from Radix Paeoniae Alba by DEAE-52 cellulose chromatography and G-25 sephadex. According to physicochemical methods, NMR and methylation analysis, RPAPW1 and RPAPW2 were established to be α-glucans consisting of predominant 4-linked α- Glc residues branched at O-6 and contained trace amount of protein and uronic acid. Immunological tests indicated that RPAPW1, RPAPW2 and could promote splenocyte proliferation and RAW264.7 phagocytic activity. In vitro, RPAPW1 and RPAPW2 elicited a week reducing power, DPPH scavenging activity and could not protect the PC12 cells from H2O2 damage. These data implied polysaccharides RPAPW1 and RPAPW2 had the potential to be a natural immunopotentiating and antioxidant supplement for preparing functional foods and nutraceuticals.
authors.affiliation: College of science, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Wuxia
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of life science, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of science, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.affiliation: College of science, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Niu
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
authors.forename: Songshan
authors.initials: S
authors.lastname: Shi
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. shunchunwang@126.com.
authors.forename: Shunchun
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of science, Northwest A&F University, Yangling, 712100, Shaanxi, China. jduan@nwsuaf.edu.cn.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Duan
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D008297:Male; D051379:Mice; D016716:PC12 Cells; D029596:Paeonia / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000276:immunology*
chemical_list: D011134:Polysaccharides
keywords: Antioxidant activity; Immunological activity; Polysaccharides; Radix Paeoniae Alba