title: LncRNA TUG1 acts as a tumor suppressor in human glioma by promoting cell apoptosis.
abstract: Previous studies have revealed multiple functional roles of long non-coding RNA taurine upregulated gene 1 in different types of malignant tumors, except for human glioma. Here, it was designed to study the potential function of taurine upregulated gene 1 in glioma pathogenesis focusing on its regulation on cell apoptosis. The expression of taurine upregulated gene 1 in glioma tissues was detected by quantitative RT-PCR and compared with that in adjacent normal tissues. Further correlation analysis was conducted to show the relationship between taurine upregulated gene 1 expression and different clinicopathologic parameters. Functional studies were performed to investigate the influence of taurine upregulated gene 1 on apoptosis and cell proliferation by using Annexin V/PI staining and cell counting kit-8 assays, respectively. And, caspase activation and Bcl-2 expression were analyzed to explore taurine upregulated gene 1-induced mechanism. taurine upregulated gene 1 expression was significantly inhibited in glioma and showed significant correlation with WHO Grade, tumor size and overall survival. Further experiments revealed that the dysregulation of taurine upregulated gene 1 affected the apoptosis and cell proliferation of glioma cells. Moreover, taurine upregulated gene 1 could induce the activation of caspase-3 and-9, with inhibited expression of Bcl-2, implying the mechanism in taurine upregulated gene 1-induced apoptosis. taurine upregulated gene 1 promoted cell apoptosis of glioma cells by activating caspase-3 and -9-mediated intrinsic pathways and inhibiting Bcl-2-mediated anti-apoptotic pathways, acting as a tumor suppressor in human glioma. This study provided new insights for the function of taurine upregulated gene 1 in cancer biology, and suggested a potent application of taurine upregulated gene 1 overexpression for glioma therapy.
authors.affiliation: Department of Neurosurgery, The Affiliated XuZhou Hospital of Medical College of Southeast University, Xuzhou 221009, Jiangsu, China lijundoc@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The Affiliated XuZhou Hospital of Medical College of Southeast University, Xuzhou 221009, Jiangsu, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Affiliated XuZhou Hospital of Medical College of Southeast University, Xuzhou 221009, Jiangsu, China.
authors.forename: Gang
authors.initials: G
authors.lastname: An
authors.affiliation: Department of Neurosurgery, The Affiliated XuZhou Hospital of Medical College of Southeast University, Xuzhou 221009, Jiangsu, China.
authors.forename: Qingfang
authors.initials: Q
authors.lastname: Ma
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000032:analysis; D053453:Caspase 9 / Q000032:analysis; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D062085:RNA, Long Noncoding / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D016019:Survival Analysis
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D062085:RNA, Long Noncoding; C000598536:TUG1 long noncoding RNA, human; D053148:Caspase 3; D053453:Caspase 9
keywords: Bcl-2; Taurine upregulated gene 1; caspase; cell apoptosis; human glioma