title: Statistical Optimization of the Content Composition Precursors Using Response Surface Methodology to Enhance Agaricoglyceride A Production from the Shaggy Ink Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes) Mycelia.
abstract: Coprinus comatus, a novel cultivated edible mushroom, has a various of pharmacological effects due to its many active components. In this study, agaricoglycerides, a new class of fungal secondary metabolites that have strong activity against neurolysin, were isolated from C. comatus mycelia. Simultaneously, a 3-level Box-Behnken factorial design was used, combined with response surface methodology, to optimize the precursor composition of agaricoglycerides for the production of agaricoglyceride A. The model estimated that a maximal yield of agaricoglyceride A (20.105 mg/L) could be obtained when the concentrations of 4-hydroxybenzoic acid, glycerol, and methanol (MeOH) were set at 75 mg/L, 0.75 mL/L, and 0.75 mL/L, respectively. The verified experiments showed that the model was significantly consistent with the model prediction. These results showed that appropriately adding the precursors could increase the production of agaricoglyceride A.
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, P. R. China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Di
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, P. R. China.
authors.forename: Ruiguo
authors.initials: R
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, P. R. China.
authors.forename: Jingzhen
authors.initials: J
authors.lastname: Tian
mesh_terms: D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D003302:Coprinus / Q000254:growth & development / Q000378:metabolism*; D005989:Glycerides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015233:Models, Statistical; D025282:Mycelium; D012044:Regression Analysis
chemical_list: D001565:Benzoates; D005989:Glycerides; C000611317:agaricoglyceride A