title: In Vitro Validation of a Closed Device Enabling the Purification of the Fluid Portion of Liposuction Aspirates.
abstract: BACKGROUND
  Adipose tissue harvested through lipoaspiration is widely exploited in plastic and cosmetic surgery, because of its remarkable trophic properties, especially relying on the presence of adipose-derived stem cells. The common procedures for adipose-derived stem cell isolation are mainly based on tissue fractionation and enzymatic digestion, requiring multiple hours of uninterrupted work, unsuitable for direct surgical applications. Recent studies demonstrated the feasibility of isolating adipose stromal cells without the need for enzymatic digestion. These studies reported the processing of the fluid portion of liposuctioned adipose tissue (lipoaspirate fluid), which contains a significant amount of progenitor cells endowed with plastic and trophic features. In this article, the authors introduce a brand new closed device--the MyStem EVO kit--which allows nonenzymatic tissue separation and rapid isolation of lipoaspirate fluid from human liposuctioned adipose tissue.
  
  
  METHODS
  Adipose tissue was liposuctioned from 14 donors, split into aliquots, and alternatively processed using either centrifugation or the MyStem EVO kit, to separate fatty and lipoaspirate fluid portions. The samples were analyzed comparatively by flow cytometry, histology, and differentiation assays. Osteoinductive and angioinductive features were analyzed through in vitro co-culture assays.
  
  
  RESULTS
  The alternative procedures enabled comparable yields; the kit rapidly isolated lipoaspirate fluid comprising a homogenous cell population with adipose stem cell immunophenotype, bilineage potential, and efficient osteoinductive and angioinductive features.
  
  
  CONCLUSIONS
  MyStem EVO allows the rapid isolation of lipoaspirate fluid with trophic properties within a closed system, and is potentially useful for regenerative medicine applications.
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cicione
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Taranto
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Marta
authors.initials: M
authors.lastname: Barba
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Isgrò
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Alessio
authors.initials: A
authors.lastname: D'Alessio
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Daniele
authors.initials: D
authors.lastname: Cervelli
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Fabio V
authors.initials: FV
authors.lastname: Sciarretta
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Sandro
authors.initials: S
authors.lastname: Pelo
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Michetti
authors.affiliation: Rome, Italy.|From the Institutes of Anatomy and Cell Biology, Biochemistry and Clinical Biochemistry, and Histology and Embryology, and the Maxillofacial Surgery Unit, Complesso Integrato Columbus, Università Cattolica del Sacro Cuore; the Clinica Nostra Signora della Mercede; and the Latium Musculoskeletal Tissue Bank.
authors.forename: Wanda
authors.initials: W
authors.lastname: Lattanzi
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002498:Centrifugation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015187:Lipectomy; D008297:Male; D013234:Stem Cells; D050151:Subcutaneous Fat / Q000166:cytology*