title: Association of the XPD and XRCC3 gene polymorphisms with oral squamous cell carcinoma in a Northeastern Brazilian population: A pilot study.
abstract: OBJECTIVE
  to evaluate the association between XPD and XRCC3 polymorphisms and oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  the sample consisted of 54 cases of OSCC and 40 cases of inflammatory fibrous hyperplasia (IFH). Genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  XPD-Lys/Gln was more common in IFH (n=28; 70%) than in OSCC (n=24; 44.4%) (OR: 0.3; p<0.05). XPD-Gln was more frequent in high-grade lesions (0.48) than in low-grade lesions (0.21) (OR: 3.4; p<0.05). The Gln/Gln genotype was associated with III and IV clinical stages (OR: 0.07; p<0.05). XRCC3-Met was more frequent in OSCC (0.49) than in IFH (0.35) (OR: 2.6; p<0.05). The Met/Met genotype was associated with the presence of metastases (OR: 8.1; p<0.05) and with III and IV clinical stages (OR: 0.07; p<0.05).
  
  
  CONCLUSIONS
  in this sample, the frequency of XPD-Gln in IFH suggests that this variant may protect against OSCC. The presence of the XRCC3-Met allele seems to contribute to the development of OSCC, metastases and more advanced stages in these lesions.
authors.affiliation: Departament of Pathology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Joabe
authors.initials: J
authors.lastname: dos Santos Pereira
authors.affiliation: Department of Cellular Biology and Genetics, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Fabrícia Lima
authors.initials: FL
authors.lastname: Fontes
authors.affiliation: Department of Cellular Biology and Genetics, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Silvia Regina Batistuzzo
authors.initials: SR
authors.lastname: de Medeiros
authors.affiliation: Post-Graduation Program of Oral Pathology, Departament of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Roseana
authors.initials: R
authors.lastname: de Almeida Freitas
authors.affiliation: Post-Graduation Program of Oral Pathology, Departament of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Lélia Batista
authors.initials: LB
authors.lastname: de Souza
authors.affiliation: Post-Graduation Program of Oral Pathology, Departament of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. Electronic address: mccmiguel@hotmail.com.
authors.forename: Márcia Cristina
authors.initials: MC
authors.lastname: da Costa Miguel
mesh_terms: D001938:Brazil; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D000077195:Squamous Cell Carcinoma of Head and Neck; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C110901:X-ray repair cross complementing protein 3; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human
keywords: DNA repair; Genetic polymorphisms; Oral cancer; Restriction fragment length polymorphisms; Squamous cell carcinoma