title: Effects of exogenous neurotrophin-3 on myocyte apoptosis and Ca(2+)-ATP enzyme levels following nerve injury in rats.
abstract: This study aims to determine the influence of neurotrophin-3 (NT-3) plasmids on neuronal apoptosis and Ca(2+)-ATP enzyme levels in injured muscles. We also investigated the mechanism underlying the role of NT-3 in delaying muscle atrophy following a peripheral nerve injury. Sixty adult Wistar rats were used to generate the peripheral nerve injury models. The rats were randomly assigned to the saline and NT-3 groups. Related indicators, such as caspase-3 protein expression, skeletal muscle cell apoptosis, and Ca(2+)-ATP enzyme expression were quantified. The expression levels of caspase-3 and the histone-muscle cell apoptosis rate in the NT-3 group decreased at different post-operative times following peripheral nerve injury, whereas NT-3 expression and the sarcoplasmic reticulum Ca(2+)-ATP enzyme levels increased. Statistically significant differences were observed in the NT-3 group as compared to the saline group (P < 0.05). NT-3 mitigated muscle atrophy following peripheral nerve damage by inhibiting caspase-3 gene expression and increasing Ca(2+)-ATP enzymatic activity, ultimately reducing muscle apoptosis.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Dong
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Yi-Lei
authors.initials: YL
authors.lastname: Zhao
keywords: Caspase-3; Neurotrophin 3; mechanism; muscle cell apoptosis; peripheral nerve injury