title: A metabolomic study of fipronil for the anxiety-like behavior in zebrafish larvae at environmentally relevant levels.
abstract: Field residue of fipronil can interfere with the physiological characters of the domesticated fish; thus, lethal dose test and the general biomarker cannot delineate the low-level situation. Manipulating by video track, we observed an anxiety-like behavior including high speed and abnormal photoperiod accommodation after exposure to fipronil at environmental typical dose in zebrafish larvae. Examining the unbiased metabolomic profiles, we found perturbation in several metabolic pathways, including the increased contents of fatty acids and glycerol and the decreased levels of the glycine, serine, and branched amino acid. We presumed that observed enhanced fatty acid utility was in response to increase energy demands caused by anxiety like behavior. Additionally, the body burden of neurotransmitter such as glycine and L-glutamate may concurrently stimulate the swimming behavior. The insight of this study showed that integral perturbation such as metabolism helps us to further understand the risk to aquatic fish at the environmentally relevant levels.
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China; Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Wang
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310018, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Zhuoyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: zhaomr@zjut.edu.cn.
authors.forename: Meirong
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001007:Anxiety; D001522:Behavior, Animal / Q000187:drug effects*; D004777:Environment; D007306:Insecticides / Q000032:analysis / Q000378:metabolism*; D007814:Larva; D055432:Metabolomics; D011720:Pyrazoles / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D007306:Insecticides; D011720:Pyrazoles; D014874:Water Pollutants, Chemical; C082360:fipronil
keywords: Fipronil; Locomotion; Low-level; Metabolomics; Zebrafish