title: Effects of Chromosomal Integration of the Vitreoscilla Hemoglobin Gene (vgb) and S-Adenosylmethionine Synthetase Gene (metK) on ε-Poly-L-Lysine Synthesis in Streptomyces albulus NK660.
abstract: ε-Poly-L-lysine (ε-PL) is a widely used natural food preservative. To test the effects of the Vitreoscilla hemoglobin (VHb) and S-adenosylmethionine (SAM) on ε-PL synthesis in Streptomyces albulus NK660, the heterologous VHb gene (vgb) and SAM synthetase gene (metK) were inserted into the S. albulus NK660 chromosome under the control of the constitutive ermE* promoter. CO-difference spectrum analysis showed S. albulus NK660-VHb strain could express functional VHb. S. albulus NK660-VHb produced 26.67 % higher ε-PL and 14.57 % higher biomass than the wild-type control, respectively. Reversed-phase high-pressure liquid chromatography (RP-HPLC) results showed the overexpression of the metK gene resulted in increased intracellular SAM synthesis in S. albulus NK660-SAM, which caused increases of biomass as well as the transcription level of ε-PL synthetase gene (pls). Results indicated that the expression of vgb and metK gene improved on ε-PL synthesis and biomass for S. albulus NK660, respectively.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Geng
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 94 Weijin Road, Tianjin, 300071, China. wangshufang@nankai.edu.cn.
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China. songcj@nankai.edu.cn.
authors.forename: Cunjiang
authors.initials: C
authors.lastname: Song
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D005285:Fermentation; D005520:Food Preservatives; D018014:Gene Transfer Techniques; D008716:Methionine Adenosyltransferase / Q000235:genetics*; D011107:Polylysine / Q000096:biosynthesis* / Q000235:genetics; D012436:S-Adenosylmethionine / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D054793:Truncated Hemoglobins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D005520:Food Preservatives; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D011107:Polylysine; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase
keywords: S-adenosylmethionine synthetase gene; Streptomyces albulus NK660; Vitreoscilla hemoglobin gene; ε-poly-L-lysine