title: The cloning, expression and purification of recombinant human neuritin from Escherichia coli and the partial analysis of its neurobiological activity.
abstract: Neuritin (Nrn1) is a neurotrophic factor that plays various roles in neural development and synaptic plasticity. In this study, the NRN1 gene was cloned and expressed in Escherichia coli and then recombinant neuritin protein was purified so that its neurobiological activity could be evaluated. The protein, which was obtained at a concentration of 0.45 mg/ml and > 90% purity, had the predicted molecular weight of 30 kDa, as determined via sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Western blot analysis confirmed that an anti-neuritin antibody could recognize the fusion protein. Subsequent functional analyses revealed that recombinant neuritin promoted neurite outgrowth in embryonic chicken dorsal root ganglia and PC12 cells. These results suggest that recombinant neuritin protein could be a valuable tool for inducing neurite regeneration, for instance in cases of spinal cord injury or neurological diseases.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Wumei
authors.initials: W
authors.lastname: Yuan
authors.forename: Na
authors.initials: N
authors.lastname: Yu
authors.forename: Xing
authors.initials: X
authors.lastname: Luo
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D058851:GPI-Linked Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D058851:GPI-Linked Proteins; C484539:NRN1 protein, human; D009479:Neuropeptides; D011994:Recombinant Proteins