title: In vitro effects of recombinant adenovirus-mediated bone morphogenetic protein 2/vascular endothelial growth factor 165 on osteogenic differentiation of bone marrow mesenchymal stem cells.
abstract: Mouse bone marrow mesenchymal stem cells C3H10T1/2 were divided into Ad-BMP2 (bone morphogenetic protein 2) group, Ad-VEGF165 (vascular endothelial growth factor 165) group, Ad-VEGF165 + Ad-BMP2 group, empty adenovirus group and control group. BMP2 and VEGF165 were highly co-expressed in Ad-VEGF165 + Ad-BMP2 group. Ad-BMP2 and Ad-VEGF165 + Ad-BMP2 groups, especially the latter (P < 0.05), had significantly higher expression levels of osteocalcin, osteoprotegerin, and osteopontin mRNA and OPN protein (P < 0.05). Ad-VEGF165 + Ad-BMP2 group had highest alkaline phosphatase (ALP) activity, strongest ALP staining and most calcium salt deposits (P < 0.05). Combining VEGF165 obviously enhanced the inducing effects of BMP2 on osteogenic differentiation capacity of C3H10T1/2 cells.
authors.affiliation: a Department of Orthopedics , General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Han
authors.affiliation: a Department of Orthopedics , General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Liu
authors.affiliation: b First Department of Leading-Treating , General Hospital of Shenyang Military Area Command of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Na
authors.initials: N
authors.lastname: Liang
authors.affiliation: c Laboratory of Severe and War-Related Trauma Center , General Hospital of Shenyang Military Area Command of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Yun-En
authors.initials: YE
authors.lastname: Liu
authors.affiliation: c Laboratory of Severe and War-Related Trauma Center , General Hospital of Shenyang Military Area Command of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Yu-Biao
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: a Department of Orthopedics , General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Zhou
authors.affiliation: a Department of Orthopedics , General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA , Shenyang , Liaoning Province , China.
authors.forename: Liang-Bi
authors.initials: LB
authors.lastname: Xiang
mesh_terms: D000256:Adenoviridae; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation; D002460:Cell Line; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010012:Osteogenesis; D014161:Transduction, Genetic; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse
keywords: Adenovirus; bone morphogenetic protein 2; osteogenic differentiation; vascular endothelial growth factor 165