title: Antioxidant and Anti-inflammatory Activities of Essential Oil and Extracts of Piper miniatum.
abstract: The chemical composition of the essential oil and antioxidant and anti-inflammatory activities of the extracts from Piper miniatum were determined. GC and GC-MS analysis of the essential oil resulted in the identification of 64 components, accounting for 89.2% of the total. The major components were caryophyllene oxide (20.3%) and α-cubebene (10.4%). The antioxidant activity was evaluated by β-carotene/linoleic acid bleaching, DPPH radical scavenging and total phenolic content. In the β-carotene assay, the n-hexane extract showed the highest inhibition activity with 42.7%, while the oil gave 91.3%. The essential oil and extracts were tested for anti-inflammatory activity by using the TPA-induced mouse ear edema model and lipoxygenase assays. The essential oil exhibited significant activity in both models as an anti-inflammatory agent. The n-hexane extract showed strong activity with inhibition of 85.9% in the TPA-induced mouse ear edema model, while the chloroform extract showed the highest activity with 94.2% in the lipoxygenase assay.
authors.forename: Wan Mohd Nuzul Hakimi
authors.initials: WM
authors.lastname: Salleh
authors.forename: Mohd Fariz
authors.initials: MF
authors.lastname: Kammil
authors.forename: Farediah
authors.initials: F
authors.lastname: Ahmad
authors.forename: Hasnah Mohd
authors.initials: HM
authors.lastname: Sirat
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D004487:Edema / Q000188:drug therapy* / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D051379:Mice; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000737:chemistry; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D009822:Oils, Volatile; D010936:Plant Extracts