title: Role of Glutathione System Redox Potential in Apoptosis Dysregulation in MCF-7 Breast Adenocarcinoma.
abstract: MCF-7 breast cancer cells and HBL-100 breast epithelial cells were cultured with N-ethylmaleimide, a blocker of SH groups. Changes in redox potential of the glutathione system, activities of glutathione reductase, glutathione peroxidase, and intensity of apoptotic cell death were evaluated. The results indicate that incubation with N-ethylmaleimide led to glutathione system imbalance, reduced tumor cell redox potential, and induced their programmed death, which seemed useful for prospective target therapy of tumor diseases.
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia. shaxristova@yandex.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Shakhristova
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Stepovaya
authors.affiliation: Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Ryazantseva
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: O L
authors.initials: OL
authors.lastname: Nosareva
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: V D
authors.initials: VD
authors.lastname: Yakushina
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Ivanov
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Novitskii
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D010084:Oxidation-Reduction / Q000187:drug effects; D011446:Prospective Studies
chemical_list: D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D005033:Ethylmaleimide
keywords: apoptosis; breast adenocarcinoma; oxidative stress; redox regulation; tumor Progress