title: PFTK1 regulates cell proliferation, migration and invasion in epithelial ovarian cancer.
abstract: PFTK1, also named Cyclin-Dependent Kinase 14 (CDK14), is a member of the cell division cycle 2 (CDC2)-related protein kinase family. It is a serine/threonine-protein kinase involved in the regulation of cell cycle progression and cell proliferation. In this study, we investigated the role of PFTK1 in epithelial ovarian cancer (EOC) development. The expression of PFTK1 was detected by Western blot and immunohistochemistry staining, both of which demonstrated that PFTK1 was overexpressed in EOC tissues and cells. Statistical analysis showed the expression of PFTK1 was associated with multiple clinicopathological factors, including tumor grade, FIGO stage, lymph node metastatis, Ki-67 expression and predicted a poor prognosis of EOC patients. With in vitro studies we found that PFTK1 expression was decreased in serum-starved ovarian cancer cells, and progressively increased after serum-re-feeding. Knocking PFTK1 down by small interfering RNA (siRNA) significantly inhibited ovarian cancer cell proliferation, migration and invasion. Taken together, our study suggested that PFTK1 played an important role in ovarian cancer development.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Gynecologic Oncology, Nantong University Cancer Hospital, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xi
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Lianxin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China. Electronic address: ntfyzjx@163.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China. Electronic address: ntfydy@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000328:Adult; D000368:Aged; D000077216:Carcinoma, Ovarian Epithelial; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D034741:RNA, Small Interfering
chemical_list: D034741:RNA, Small Interfering; C515076:CDK14 protein, human; D018844:Cyclin-Dependent Kinases
keywords: Epithelial ovarian cancer; Overexpression; PFTK1