title: Expression and mechanism of microRNA-181A on incidence and survival in late liver metastases of colorectal cancer.
abstract: The emerging role of microRNA-181A (miR-181A) in CRC patients with late liver metastases was studied. In the present study we investigated the association between expression and mechanism of miRNA-181A, liver metastasis and prognosis of colorectal cancer (CRC). The expression of miR-181A and PTEN in CRC patients with late liver metastases was higher than that of the normal (control) group, whereas the expression of miR-181A and PTEN was lower in all pathological groups (TNM I-TNM IV). Overall survival (OS) of lower expression miR-181A CRC patients with late liver metastases was higher than that of higher expression miR-181A CRC patients with late liver metastases. The expression of miR-181A and PTEN in the colon cell line NCM460 was lower than that of the colon cancer SW620 cell line. Upregulation of miR-181A promoted cell viability and inhibited apoptosis of SW620 cells, suppressed PTEN expression and activated phosphorylation of AKT (p-AKT) in SW620 cells. Additionally, downregulation of miR-181A inhibited cell viability of SW620 cells through promotion of PTEN and inhibition of p-AKT. Together, our results indicate that miR-181A expression is associated with CRC patients with late liver metastases through PTEN/AKT signaling.
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Science, Jinan, Shandong 250117, P.R. China.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Science, Jinan, Shandong 250117, P.R. China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Science, Jinan, Shandong 250117, P.R. China.
authors.forename: Zhongfa
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Science, Jinan, Shandong 250117, P.R. China.
authors.forename: Yanlai
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Science, Jinan, Shandong 250117, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Han
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human