title: In vitro release and In vivo biocompatibility studies of biomimetic multilayered alginate-chitosan/β-TCP scaffold for osteochondral tissue.
abstract: Biomimetic three-layered monolithic scaffold (TLS) intended for the treatment of osteocondral defects was prepared by using alginate, chitosan and β-tricalcium phosphate (β-TCP) to study drug release behavior of the alternative drug delivery system and to investigate the therapeutic efficacy of the scaffold. Dexamethasone sodium phosphate (Dex) as a model drug was incorporated into the scaffold by solvent sorption method and in vitro release studies were conducted. In addition, the scaffold was implanted into the defects formed in the trochlea of Sprague-Dawley rats to assess the healing potential of the TLS on the osteochondral defect against reference Maioregen® comparatively. The release studies showed that after an initial burst at 3rd h, dexamethasone is released slowly during a 72-h period. In vivo studies indicated that the TLS has good tissue biocompatibility and biodegradation rate and showed better results during osteochondral healing process compared to the reference. All results demonstrated that the alginate-chitosan/β-TCP scaffold could be evaluated as a good candidate for osteochondral tissue applications.
authors.affiliation: a Faculty of Pharmacy, Department of Pharmaceutical Technology , Yeditepe University , Istanbul , Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Algul
authors.affiliation: b Department of Ortopedic Prothestic and Orthotics , Nisantasi University , Istanbul , Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Gokce
authors.affiliation: c Department of Orthopedics and Traumatology , Agrı Government Hospital , Agrı , Turkey.
authors.forename: Ayberk
authors.initials: A
authors.lastname: Onal
authors.affiliation: d Department of Orthopedics and Traumatology , Medicalpark Gaziantep Hospital , Gaziantep , Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Servet
authors.affiliation: e Department of Pathology, School of Medicine , Yeditepe University , Istanbul , Turkey.
authors.forename: Asiye Isın
authors.initials: AI
authors.lastname: Dogan Ekici
authors.affiliation: f Faculty of Pharmacy, Department of Pharmaceutical Technology , Istanbul University , Istanbul , Turkey.
authors.forename: Fatma Gulgun
authors.initials: FG
authors.lastname: Yener
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D002356:Cartilage / Q000166:cytology / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003907:Dexamethasone / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D065546:Drug Liberation; D005269:Femur / Q000166:cytology / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D008297:Male; D008422:Materials Testing; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D002130:Calcium Phosphates; D004337:Drug Carriers; D006603:Hexuronic Acids; D012997:Solvents; C485817:beta-tricalcium phosphate; D003907:Dexamethasone; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Release; alginate; chitosan; osteochondral defect; scaffold