title: Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae.
abstract: In Saccharomyces cerevisiae, the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely eliminated in this mutant, so alternative transport systems must exist. Deletion of MRS3, a component of the iron import machinery, also causes a copper-dependent growth defect on non-fermentable carbon. Deletion of both PIC2 and MRS3 led to a more severe respiratory growth defect than either individual mutant. In addition, MRS3 expressed from a high copy number vector was able to suppress the oxygen consumption and copper uptake defects of a strain lacking PIC2. When expressed in Lactococcus lactis, Mrs3 mediated copper and iron import. Finally, a PIC2 and MRS3 double mutant prevented the copper-dependent activation of a heterologously expressed copper sensor in the mitochondrial intermembrane space. Taken together, these data support a role for the iron transporter Mrs3 in copper import into the mitochondrial matrix.
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Katherine E
authors.identifier: http://orcid.org/0000-0002-3391-8649
authors.initials: KE
authors.lastname: Vest
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Micah G
authors.initials: MG
authors.lastname: Gammon
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Margaret K
authors.initials: MK
authors.lastname: Maynard
authors.affiliation: College of Charleston, Charleston, SC 29424, USA.
authors.forename: Olivia L
authors.initials: OL
authors.lastname: White
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Jai A
authors.initials: JA
authors.lastname: Cobine
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Wilkerson K
authors.initials: WK
authors.lastname: Mahone
authors.affiliation: Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA paul.cobine@auburn.edu.
authors.forename: Paul A
authors.identifier: http://orcid.org/0000-0001-6012-0985
authors.initials: PA
authors.lastname: Cobine
mesh_terms: D016880:Anisotropy; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D017353:Gene Deletion; D017930:Genes, Reporter; D007501:Iron / Q000378:metabolism*; D013294:Lactococcus lactis / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D012834:Silver / Q000633:toxicity; D013050:Spectrometry, Fluorescence
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D012834:Silver; D003300:Copper; D007501:Iron
keywords: copper; cytochrome c oxidase; iron; mitochondrial carrier family