title: Cloning, Expression, Purification, Regulation, and Subcellular Localization of a Mini-protein from Campylobacter jejuni.
abstract: The Cj1169c-encoded putative protein of Campylobacter was expressed and purified from E. coli after sequence optimization. The purified protein allowed the production of a specific rabbit antiserum that was used to study the protein expression in vitro and its subcellular localization in the bacterial cell and putative interactions with other proteins. This protein is produced in Campylobacter and it clearly localizes into the periplasmic space. The level of protein production depends on factors, including pH, temperature, osmolarity, and growth phase suggesting a role in the Campylobacter environmental adaptation. The cysteine residues present in the sequence are probably involved in disulfide bridges, which may promote covalent interactions with other proteins of the Campylobacter envelope.
authors.affiliation: UMR-MD1 Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France.
authors.forename: Soumeya
authors.initials: S
authors.lastname: Aliouane
authors.affiliation: UMR-MD1 Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Pagès
authors.affiliation: UMR-MD1 Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France. jean-michel.bolla@univ-amu.fr.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Bolla
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D019897:Periplasm / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins