title: Creating Hierarchical Topographies on Fibrous Platforms Using Femtosecond Laser Ablation for Directing Myoblasts Behavior.
abstract: Developing an artificial extracellular matrix that closely mimics the native tissue microenvironment is important for use as both a cell culture platform for controlling cell fate and an in vitro model system for investigating the role of the cellular microenvironment. Electrospinning, one of the methods for fabricating structures that mimic the native ECM, is a promising technique for creating fibrous platforms. It is well-known that align or randomly distributed electrospun fibers provide cellular contact guidance in a single pattern. However, native tissues have hierarchical structures, i.e., topographies on the micro- and nanoscales, rather than a single structure. Thus, we fabricated randomly distributed nanofibrous (720 ± 80 nm in diameter) platforms via a conventional electrospinning process, and then we generated microscale grooves using a femtosecond laser ablation process to develop engineered fibrous platforms with patterned hierarchical topographies. The engineered fibrous platforms can regulate cellular adhesive morphology, proliferation, and distinct distribution of focal adhesion proteins. Furthermore, confluent myoblasts cultured on the engineered fibrous platforms revealed that the direction of myotube assembly can be controlled. These results indicate that our engineered fibrous platforms may be useful tools in investigating the roles of nano- and microscale topographies in the communication between cells and ECM.
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.
authors.forename: Indong
authors.initials: I
authors.lastname: Jun
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.|Department of Mechanical Engineering, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Yong-Woo
authors.initials: YW
authors.lastname: Chung
authors.affiliation: Department of Bioengineering, Hanyang University , Seoul 04763, Republic of Korea.|BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team , Seoul 133-791, Republic of Korea.
authors.forename: Yun-Hoe
authors.initials: YH
authors.lastname: Heo
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.
authors.forename: Hyung-Seop
authors.initials: HS
authors.lastname: Han
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.
authors.forename: Jimin
authors.initials: J
authors.lastname: Park
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.|Department of Mechanical Engineering, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Hongsoo
authors.initials: H
authors.lastname: Jeong
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, Hanyang University , Seoul 04763, Republic of Korea.|BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team , Seoul 133-791, Republic of Korea.
authors.forename: Yu Bin
authors.initials: YB
authors.lastname: Lee
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.|Korea University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.|Korea University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Hyun-Kwang
authors.initials: HK
authors.lastname: Seok
authors.affiliation: Department of Bioengineering, Hanyang University , Seoul 04763, Republic of Korea.|BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team , Seoul 133-791, Republic of Korea.
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science & Technology , Seoul 02792, Republic of Korea.|Korea University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Hojeong
authors.initials: H
authors.lastname: Jeon
mesh_terms: D032701:Biomimetics; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D060833:Cellular Microenvironment; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure*; D032446:Myoblasts / Q000737:chemistry / Q000648:ultrastructure*; D023822:Tissue Engineering
keywords: ECM (extracellular matrix); electrospinning; femtosecond laser; hierarchical topographies; nano/micro scale