title: Assessment of the circumferential margins, extraprostatic extension and Gleason score in radical prostatectomy specimens: Comparison of a partial embedding method with supplemental total inclusion of peripheral tissues.
abstract: BACKGROUND
  Recent data suggest that up to 21% of positive circumferential margins (PCM) and 47% of extraprostatic extension (EPE) samples may be missed when partial embedding methods are employed. Kim and colleagues (2009) suggested that total inclusion of the periphery (3mm rim) of the prostate prevented the failure to detect PCM and EPE.
  
  
  METHODS
  Radical prostatectomy specimen (n=148) slides were reviewed after adoption of a protocol that included a ∼3 mm rim of peripheral tissues. We evaluated whether the analysis of supplemental slides of prostate periphery changed margin status, presence of EPE, Gleason score and extent of PCM and EPE.
  
  
  RESULTS
  Partial sampling resulted in missing 29% of PCM and 20% of EPE without using data from the supplemental slides of prostate periphery. Changes from focal to extensive disease were found in 11/21 (52%) cases of positive circumferential margins and in 5/13 (38%) cases of extraprostatic extension. Changes in the Gleason score were uncommon.
  
  
  CONCLUSIONS
  These results indicate the importance of including all the prostate peripheral tissue for microscopic analysis when partial embedding methods are adopted.
authors.affiliation: Hospital Universitário Professor Edgard Santos, Rua Augusto Viana sn, Canela, Salvador 40110060, Brazil.
authors.forename: Luiza Cavalcanti
authors.initials: LC
authors.lastname: Fadul
authors.affiliation: Department of Pathology, Faculty of Medicine, Largo do Terreiro de Jesus s/n, Federal University of Bahia, 40025010 Salvador, Brazil.
authors.forename: Mariana Trindade
authors.initials: MT
authors.lastname: da Silva
authors.affiliation: Hospital Universitário Professor Edgard Santos, Rua Augusto Viana sn, Canela, Salvador 40110060, Brazil; Department of Pathology, Faculty of Medicine, Largo do Terreiro de Jesus s/n, Federal University of Bahia, 40025010 Salvador, Brazil; Gonçalo Moniz Research Center, Rua Waldemar Falcão, 121, Oswaldo Cruz Foundation, 40296710 Salvador, Brazil; Imagepat, Pathology Laboratory, Rua Altino Serbeto de Barros 41810570, 119 Salvador, Brazil.
authors.forename: Luiz Antonio Rodrigues
authors.initials: LA
authors.lastname: de Freitas
authors.affiliation: Hospital Universitário Professor Edgard Santos, Rua Augusto Viana sn, Canela, Salvador 40110060, Brazil; Department of Pathology, Faculty of Medicine, Largo do Terreiro de Jesus s/n, Federal University of Bahia, 40025010 Salvador, Brazil; Gonçalo Moniz Research Center, Rua Waldemar Falcão, 121, Oswaldo Cruz Foundation, 40296710 Salvador, Brazil. Electronic address: daa@ufba.br.
authors.forename: Daniel Abensur
authors.initials: DA
authors.lastname: Athanazio
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D006801:Humans; D008297:Male; D060787:Neoplasm Grading; D016612:Paraffin Embedding; D011468:Prostatectomy / Q000379:methods*; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods*
keywords: Pathology; Prostatectomy; Prostatic neoplasms