title: O-Glycan analysis of cellobiohydrolase I from Neurospora crassa.
abstract: We describe here the composition of the O-linked glycans on the Neurospora crassa cellobiohydrolase I (CBHI), which accounts for approximately 40% of the protein secreted by cells growing in the presence of cellulose. CBHI is O-glycosylated with six types of linear, and three types of branched, O-glycans containing approximately equal amounts of mannose and galactose. In addition to the classic fungal O-glycans with reducing end mannoses, we also identified reducing end galactoses which suggest the existence of a protein-O-galactosyltransferase in N. crassa Because of the excellent genetic resources available for N. crassa, the knowledge of the CBHI O-glycans may enable the future evaluation of the role of O-glycosylation on cellulase function and the development of directed O-glycan/cellulase engineering.
authors.affiliation: Energy Biosciences Institute, University of California Berkeley, 2151 Berkeley Way MC 5230, Berkeley, CA 94720, USA.
authors.forename: Shu-Lun
authors.initials: SL
authors.lastname: Tang
authors.affiliation: Energy Biosciences Institute, University of California Berkeley, 2151 Berkeley Way MC 5230, Berkeley, CA 94720, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bubner
authors.affiliation: Energy Biosciences Institute, University of California Berkeley, 2151 Berkeley Way MC 5230, Berkeley, CA 94720, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Bauer
authors.affiliation: Energy Biosciences Institute, University of California Berkeley, 2151 Berkeley Way MC 5230, Berkeley, CA 94720, USA crs@berkeley.edu.
authors.forename: Chris R
authors.initials: CR
authors.lastname: Somerville
mesh_terms: D002240:Carbohydrate Sequence; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D008358:Mannose / Q000737:chemistry / Q000302:isolation & purification; D009492:Neurospora crassa / Q000737:chemistry / Q000201:enzymology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D002482:Cellulose; D043366:Cellulose 1,4-beta-Cellobiosidase; D008358:Mannose; D005690:Galactose
keywords: Neurospora crassa; cellobiohydrolase; filamentous fungi; lignocellulosic biofuel; protein O-glycosylation