title: Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection.
abstract: To create bright and stable fluorescent biolabels for immunoassay detection of mycotoxin deoxynivalenol in food and feed, CdSe/CdS/ZnS core-shell quantum dots (QDs) were encapsulated in silica nanoparticles through a water-in-oil reverse microemulsion process. The optical properties and stability of the obtained silica coated QDs (QD@SiO2), modified with amino, carboxyl and epoxy groups and stabilized with polyethylene glycol fragments, were characterized in order to assess their bioapplicability. The developed co-condensation techniques allowed maintaining 80% of the initial fluorescent properties and yielded stable fluorescent labels that could be easily activated and bioconjugated. Further, the modified QD@SiO2 were efficiently conjugated with antibodies and applied as a novel label in a microtiter plate based immunoassay and a quantitative column-based rapid immunotest for deoxynivalenol detection with IC50 of 473 and 20 ng/ml, respectively.
authors.affiliation: Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia; Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Electronic address: Valentina.Goftman@UGent.be.
authors.forename: Valentina V
authors.initials: VV
authors.lastname: Goftman
authors.affiliation: Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.
authors.forename: Tangi
authors.initials: T
authors.lastname: Aubert
authors.affiliation: IBCN/Electromagnetics Group, Department of Information Technology, Ghent University/iMinds, Sint-Pietersnieuwstraat 41, B-9000 Gent, Belgium.
authors.forename: Dries Vande
authors.initials: DV
authors.lastname: Ginste
authors.affiliation: Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.
authors.forename: Rik
authors.initials: R
authors.lastname: Van Deun
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Beloglazova
authors.affiliation: Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.
authors.forename: Zeger
authors.initials: Z
authors.lastname: Hens
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.affiliation: Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia; Saint-Petersburg State University, Universitetskii pr. 26, 198504 Saint-Petersburg, Russia.
authors.forename: Irina Yu
authors.initials: IY
authors.lastname: Goryacheva
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D005456:Fluorescent Dyes; D014255:Trichothecenes; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; C007262:deoxynivalenol
keywords: FLISA; Immunoassay.; Microemulsion; Nanocrystals; PEGylation; Surface functionalization