title: The likelihood ratio and frequency of DQ2/DQ8 haplotypes in Iranian patients with celiac disease.
abstract: OBJECTIVE
  The aim of this study was to evaluate the likelihood ratio and frequency of DQ2 and DQ8 in Iranian patients with celiac disease (CD).
  
  
  BACKGROUND
  The HLA DQ2 and HLA DQ8 are the important mediators in the development of celiac disease. A few studies evaluated the frequency of HLA DQ2 and HLA DQ8 haplotypes among the Iranian population with low sample size.
  
  
  METHODS
  In this cross-sectional study, to predict HLA-DQ2 and DQ8 haplotypes, 141(73 male, 78 female) confirmed CD patients compared to 151 healthy controls were enrolled into this study during 2013-2014. HLA DQ2/ DQ8 haplotypes was determined in cases and controls using PCR-SSP technique.
  
  
  RESULTS
  DQ2 and DQ8 were positive in 80% (n=111) and 49% (n= 69) of CD patients and 36% (n=61) and 13% (n=21) of control group respectively. Moreover, 32% (n=45) of CD patients and 5.3% (n=8) of the control group were carrier of both haplotypes. In the case group about one-third of patients (32.2%) were positive for carrying both DQ2 and DQ8 heterodimers while only 5.3% (n=8) of the control group were carrier. In addition, the positive likelihood ratio of DQ2 and DQ8 were 1.74 (CI: 1.4- 2.1), and 2.6 (CI: 1.8- 2.7), respectively.
  
  
  CONCLUSIONS
  The result of this study showed that the frequency of DQ8 among our population is higher than those reported by European countries, but it is close to those founded in South America and Middle East. This result suggests that the higher prevalence of HLA DQ8 pattern in Iranian CD patients is similar to non-European patients.
authors.affiliation: Digestive Disease Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Khosravi
authors.affiliation: Digestive Disease Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran; Health Center of Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoume
authors.initials: M
authors.lastname: Mansouri
authors.affiliation: Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rostami-Nejad
authors.affiliation: Digestive Disease Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran.
authors.forename: Bijan
authors.initials: B
authors.lastname: Shahbazkhani
authors.affiliation: Digestive Disease Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran.
authors.forename: Golnaz
authors.initials: G
authors.lastname: Ekhlasi
authors.affiliation: Gholhak Medical Laboratory. Tehran, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Kalantari
keywords: Celiac disease; HLA DQ2; HLA DQ8; HLA typing; Iranian population; Likelihood ratio