title: Development of a matrix solid-phase dispersion extraction combined with high-performance liquid chromatography for determination of five lignans from the Schisandra chinensis.
abstract: A method based on a simplified sample extraction by matrix solid phase dispersion (MSPD) followed by HPLC determination is validated for analysis of five lignans in Schisandra chinensis. The MSPD parameters that affect the extraction efficiency of lignans from S. chinensis were examined and optimized. The optimal extraction conditions were determined to be that silica gel was used as dispersing sorbent, the ratio of silica gel to sample mass was selected to be 2:1, and 4mL of methanol was used as elution solvent. The method recoveries were determined to be from 92.25 to 101.17% and the RSDs from 1.3 to 4.9%. The extraction yields of five lignans obtained by the MSPD were higher than those of traditional reflux and sonication extraction with reduced requirements on sample, solvent and time. In addition, the optimized method was applied for analyzing five real S. chinensis samples obtained from different cultivated areas.
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar 161006, China; Basic Medical Science College, Qiqihar Medical University, Qiqihar 161006, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, PR China.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar 161006, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guan
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar 161006, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar 161006, China.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Yang
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar 161006, China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Medicine and Drug Research, Qiqihar Medical University, Qiqihar 161006, China. Electronic address: caidefu1981@gmail.com.
authors.forename: Defu
authors.initials: D
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017705:Lignans / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D032141:Schisandra / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D017705:Lignans; D010936:Plant Extracts
keywords: HPLC; Lignans; Matrix solid phase dispersion; Schisandra chinensis