title: Excessive Selenium Supplementation Induced Oxidative Stress and Endoplasmic Reticulum Stress in Chicken Spleen.
abstract: Excessive selenium (Se) intake is harmful for animals and humans. The aim of the present study was to examine the effect of long-term excessive Se supplementation on oxidative stress and endoplasmic reticulum (ER) stress-related injuries in chicken spleen. A total of 180 1-day-old chickens were randomly divided into four groups with different Se dietary contents (0.2 mg/kg Se, 5 mg/kg Se, 10 mg/kg Se, or 15 mg/kg Se) for 45 days. Then, the levels of antioxidative enzymes, GPx, SOD, and MDA as well as the expression levels of GRP78, ARF6, caspase 3, caspase 12, and Bcl 2 in the spleen were determined at days 15, 30, and 45, respectively. The results showed that excessive Se treatment decreased the activities of GPx and SOD (P < 0.05) but increased the levels of MDA (P < 0.05) in a dose- and time-dependent manner. In addition, the ER stress genes GRP78 and ATF6 were highly expressed (P < 0.05), and the apoptosis genes caspase 3 and caspase 12 were increased, but Bcl 2 was decreased by Se treatment (P < 0.05). Correlation analysis showed that there was a high correlation between these biomarkers, which indicated that ER stress and ER stress-related apoptosis were correlated with oxidative stress. These results showed the important role of oxidative stress and ER stress in Se-related immune injuries in chicken.
authors.affiliation: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.|Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China. XuegangLuo@126.com.|School of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China. XuegangLuo@126.com.
authors.forename: Xuegang
authors.initials: X
authors.lastname: Luo
authors.affiliation: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China. hejiansichuan@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: He
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002645:Chickens / Q000378:metabolism*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000008:administration & dosage* / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D012643:Selenium
keywords: Apoptosis; Chicken spleen; Endoplasmic reticulum stress; Oxidative stress; Selenium