title: [Pt(O,O'-acac)(γ-acac)(DMS)] versus cisplatin: apoptotic effects in B50 neuroblastoma cells.
abstract: Cisplatin is one of the most active chemotherapeutic agents used in the treatment of childhood and adult malignancies. Cisplatin induces cell death through different pathways. Despite its effectiveness, the continued clinical use of cisplatin is limited by onset of severe side effects (nephrotoxicity, ototoxicity and neurotoxicity) and drug resistance. Therefore, one of the main experimental oncology purpose is related to the search for new platinum-based drugs to create different types of adducts or more specific and effective subcellular targets. Thus, [Pt(O,O'-acac)(γ-acac)(DMS)], which reacts preferentially with protein thiols or thioether, was synthesized. In our research, different approaches were used to compare cisplatin and [Pt(O,O'-acac)(γ-acac)(DMS)] effects in B50 rat neuroblastoma cells. Our results, using immunocytochemical, cytometric and morphological techniques, showed that these compounds exert a cytostatic action and activate apoptosis with different pathways. Long-term effects demonstrated that [Pt(O,O'-acac)(γ-acac)(DMS)] exerts cytotoxic effects in neuronal B50 cell line not inducing drug resistance. Analysis was performed both to compare the ability of these platinum compounds to induce cell death and to investigate the intracellular mechanisms at the basis of their cytotoxicity.
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Grimaldi
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Giada
authors.initials: G
authors.lastname: Santin
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Violetta
authors.initials: V
authors.lastname: Insolia
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Dal Bo
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Valeria Maria
authors.initials: VM
authors.lastname: Piccolini
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Veneroni
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Barni
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Verri
authors.affiliation: Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Lecce, Italy.
authors.forename: Sandra Angelica
authors.initials: SA
authors.lastname: De Pascali
authors.affiliation: Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Lecce, Italy.
authors.forename: Francesco Paolo
authors.initials: FP
authors.lastname: Fanizzi
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
authors.forename: Graziella
authors.initials: G
authors.lastname: Bernocchi
authors.affiliation: Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy. bottone@unipv.it.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bottone
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology*; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; C527556:Pt(O,O'-acac)(gamma-acac)(DMS); D002945:Cisplatin
keywords: Apoptosis; B50 neuroblastoma rat cells; Cisplatin; Cytoplasmic organelles; PtAcacDMS