title: E-cadherin-mediated contact of endothelial progenitor cells with mesenchymal stem cells through β-catenin signaling.
abstract: Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was beneficial to angiogenesis, tissue repair, and regeneration. The adhesion of MSCs and EPCs could promote the pluripotency of MSCs, particularly self-renewal and multi-differentiation to osteoblasts, chondrocytes, and adipocytes. This study focused on the mechanism of adhesion between EPCs and MSCs. The results showed that E-cadherin (E-cad) mediated the adhesion of MSCs and EPCs through the E-cad/beta-catenin signaling pathway. The E-cad of EPCs occupied a dominant position during this process, which activated and up-regulated the beta-catenin (β-catenin) of MSCs to improve cohesion and exert their biological function.
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.|Department of General Surgery, Xi'an Central Hospital, Xi'an, Shaanxi, 710003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Xi'an Central Hospital, Xi'an, Shaanxi, 710003, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Gao
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Jixue
authors.initials: J
authors.lastname: Hou
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Sibo
authors.initials: S
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Xuyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Quanhu
authors.initials: Q
authors.lastname: Ge
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Wan
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Cheng
authors.affiliation: The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Jinpo
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Immunology, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832002, China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.|Laboratory of Translational Medicine, School of Medicine, Shihezi University, Shihezi, Xinjiang, 832008, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D015820:Cadherins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D015854:Up-Regulation; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051176:beta Catenin
keywords: E-cadherin; adhesion; beta-catenin; siRNA; transfection