title: Structure and preventive effects against ethanol-induced gastric ulcer of an expolysaccharide from Lachnum sp.
abstract: An extracellular polysaccharide of Lachnum sp. (LEP) was purified by DEAE-cellulose 52 column chromatography and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 3.22 × 10(4)Da. The structure of LEP-2a was characterized by chemical and spectroscopic methods, including methylation analysis, periodate oxidation-smith degradation, infrared spectroscopy and NMR analysis. Results indicated that LEP-2a was a (1→3)-,(1→6)-β-D-Glcp, whose branch chain was consist of two d-glucopyranosyl residues linked by β-1,3-glycosidic linkage, which was linked at C6 of the backbone chain by β-1,6-glycosidic linkage. To study the protective effects of LEP-2a on the ethanol-induced gastric ulcer in mice, LEP-2a (100, 200 and 400mg/kg/d) was given to mice by gavage for 2 weeks. Results showed that LEP-2a significantly decreased the ulcer bleeding areas, pepsin activity, gastric juice volume, gastric juice total acidity and the malondialdehyde (MDA) content in serum. Meanwhile, the superoxide dismutase (SOD) increased significantly. The above findings suggested that LEP-2a had a significant preventive effect against the ethanol-induced gastric ulcer.
authors.affiliation: Microbial Resources and Application Laboratory, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Microbial Resources and Application Laboratory, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Microbial Resources and Application Laboratory, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors.forename: Ru-Yue
authors.initials: RY
authors.lastname: Yuan
authors.affiliation: Microbial Resources and Application Laboratory, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors.forename: Zi-Yang
authors.initials: ZY
authors.lastname: Ye
authors.affiliation: Microbial Resources and Application Laboratory, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors.forename: Hui-Ran
authors.initials: HR
authors.lastname: Ye
authors.affiliation: Microbial Resources and Application Laboratory, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address: yeming123@sina.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D006031:Glycosylation; D008297:Male; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000897:Anti-Ulcer Agents; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D000431:Ethanol
keywords: (1→3)-,(1→6)-β; -D-Glcp; Ethanol-induced gastric ulcer; Extracellular polysaccharide