title: GLP-2 Prevents Intestinal Mucosal Atrophy and Improves Tissue Antioxidant Capacity in a Mouse Model of Total Parenteral Nutrition.
abstract: We investigated the effects of exogenous glucagon-like peptide-2 (GLP-2) on mucosal atrophy and intestinal antioxidant capacity in a mouse model of total parenteral nutrition (TPN). Male mice (6-8 weeks old) were divided into three groups (n = 8 for each group): a control group fed a standard laboratory chow diet, and experimental TPN (received standard TPN solution) and TPN + GLP-2 groups (received TPN supplemented with 60 µg/day of GLP-2 for 5 days). Mice in the TPN group had lower body weight and reduced intestinal length, villus height, and crypt depth compared to the control group (all p < 0.05). GLP-2 supplementation increased all parameters compared to TPN only (all p < 0.05). Intestinal total superoxide dismutase activity and reduced-glutathione level in the TPN + GLP-2 group were also higher relative to the TPN group (all p < 0.05). GLP-2 administration significantly upregulated proliferating cell nuclear antigen expression and increased glucose-regulated protein (GRP78) abundance. Compared with the control and TPN + GLP-2 groups, intestinal cleaved caspase-3 was increased in the TPN group (all p < 0.05). This study shows GLP-2 reduces TPN-associated intestinal atrophy and improves tissue antioxidant capacity. This effect may be dependent on enhanced epithelial cell proliferation, reduced apoptosis, and upregulated GRP78 expression.
authors.affiliation: Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, Nanjing 210002, China. lqiuchenggd@163.com.
authors.forename: Qiucheng
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. ahbijingcheng@163.com.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Bi
authors.affiliation: Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, Nanjing 210002, China. wxinying@263.net.
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. jiangtingting08med@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. wuchao0008@126.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. easyhard666@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.affiliation: Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, Nanjing 210002, China. xuejingao870214@163.com.
authors.forename: Xuejin
authors.initials: X
authors.lastname: Gao
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wan
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. huijunzheng@163.com.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001284:Atrophy / Q000209:etiology / Q000517:prevention & control; D053148:Caspase 3 / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP; D053767:Glucagon-Like Peptide 2 / Q000494:pharmacology*; D005978:Glutathione / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D007422:Intestines / Q000187:drug effects; D008297:Male; D051379:Mice; D023421:Models, Animal; D010289:Parenteral Nutrition, Total / Q000009:adverse effects* / Q000379:methods; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects; D013482:Superoxide Dismutase / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D000091342:Endoplasmic Reticulum Chaperone BiP; D053767:Glucagon-Like Peptide 2; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D013482:Superoxide Dismutase; C505409:Casp3 protein, mouse; D053148:Caspase 3; D005978:Glutathione
keywords: GRP78; antioxidant capacity; apoptosis; glucagon-like peptide-2; proliferation