title: Optimization and modeling for the synthesis of sterol esters from deodorizer distillate by lipase-catalyzed esterification.
abstract: In this paper, cotton seed oil deodorizer distillate (CSODD), was recovered to obtain fatty acid sterol ester (FASE), which is one of the biological activated substances added as human therapeutic to lower cholesterol. Esterification reactions were carried out using Candida rugosa lipase as a catalyst, and the conversion of phytosterol was optimized using response surface methodology. The highest conversion (90.8 ± 0.4%) was reached at 0.84 wt% enzyme load, 1:25 solvent/CSODD mass ratio, and 44.2 °C after 12 H reaction. A kinetic model based on the reaction rate equation was developed to describe the reaction process. The activation energy of the reaction was calculated to be 56.9 kJ/mol and the derived kinetic parameters provided indispensable basics for further study. The optimization and kinetic research of synthesizing FASE from deodorizer distillate provided necessary information for the industrial applications in the near future. Experimental results showed that the proposed process is a promising alternative to recycle sterol esters from vegetable oil deodorizer distillates in a mild, efficient, and environmental friendly method.
authors.affiliation: State Key Laboratory of Chemical Engineering, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemical Engineering, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemical Engineering, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Aiwu
authors.initials: A
authors.lastname: Zeng
mesh_terms: D002175:Candida / Q000201:enzymology; D002784:Cholesterol / Q000378:metabolism*; D004951:Esterification; D004952:Esters / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D008049:Lipase / Q000737:chemistry; D010840:Phytosterols / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D010938:Plant Oils / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013261:Sterols / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D004952:Esters; D010840:Phytosterols; D010938:Plant Oils; D012997:Solvents; D013261:Sterols; D002784:Cholesterol; D008049:Lipase
keywords: Candida rugosa lipase; deodorizer distillate; food additives; kinetics; phytosterol ester