title: Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts.
abstract: Sexual reproduction is crucially dependent on meiosis, a conserved, specialized cell division programme that is essential for the production of haploid gametes. Here we demonstrate that fertility and the implementation of the meiotic programme require a previously uncharacterized meiosis-specific protein, MEIOC. Meioc invalidation in mice induces early and pleiotropic meiotic defects in males and females. MEIOC prevents meiotic transcript degradation and interacts with an RNA helicase that binds numerous meiotic mRNAs. Our results indicate that proper engagement into meiosis necessitates the specific stabilization of meiotic transcripts, a previously little-appreciated feature in mammals. Remarkably, the upregulation of MEIOC at the onset of meiosis does not require retinoic acid and STRA8 signalling. Thus, we propose that the complete induction of the meiotic programme requires both retinoic acid-dependent and -independent mechanisms. The latter process involving post-transcriptional regulation likely represents an ancestral mechanism, given that MEIOC homologues are conserved throughout multicellular animals.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Abby
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Tourpin
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Ribeiro
authors.affiliation: Molecular Cell Biology Group/Experimental Center, Institute of Physiological Chemistry, Medical School, MTZ, Dresden University of Technology, Fiedlerstrasse 42, Dresden 01307, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Daniel
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Messiaen
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Moison
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Guerquin
authors.affiliation: CEA, DSV/IBITEC-S/SPI/Li2D, Laboratory 'Innovative Technologies for Detection and Diagnostic', CEA-Marcoule, BP 17171, Bagnols-sur-Cèze F-30200, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gaillard
authors.affiliation: CEA, DSV/IBITEC-S/SPI/Li2D, Laboratory 'Innovative Technologies for Detection and Diagnostic', CEA-Marcoule, BP 17171, Bagnols-sur-Cèze F-30200, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Armengaud
authors.affiliation: Centre d'Ingénierie Génétique Murine, Institut Pasteur, Paris 75015, France.
authors.forename: Francina
authors.initials: F
authors.lastname: Langa
authors.affiliation: Molecular Cell Biology Group/Experimental Center, Institute of Physiological Chemistry, Medical School, MTZ, Dresden University of Technology, Fiedlerstrasse 42, Dresden 01307, Germany.
authors.forename: Attila
authors.initials: A
authors.lastname: Toth
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Martini
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR-967, BP 6, Fontenay-aux-Roses 92265, France.|CEA, DSV, iRCM, SCSR, LDG, Fontenay-aux-Roses 92265, France.|INSERM, Unité 967, Fontenay-aux-Roses F-92265, France.|Université Paris-Sud, UMR-967, Fontenay-aux-Roses F-92265, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Livera
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000378:metabolism* / Q000473:pathology; D006066:Gonads / Q000378:metabolism* / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008297:Male; D008540:Meiosis / Q000235:genetics; D049469:Meiotic Prophase I / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D014212:Tretinoin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C000607598:MEIOC protein, human; C000607599:MEIOC protein, mouse; D012333:RNA, Messenger; C102019:Stra8 protein, mouse; D014212:Tretinoin