title: Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1.
abstract: Arabidopsis thaliana leucine-rich repeat-containing (NLR) proteins RPS4 and RRS1, known as dual resistance proteins, confer resistance to multiple pathogen isolates, such as the bacterial pathogens Pseudomonas syringae and Ralstonia solanacearum and the fungal pathogen Colletotrichum higginsianum. RPS4 is a typical Toll/interleukin 1 Receptor (TIR)-type NLR, whereas RRS1 is an atypical TIR-NLR that contains a leucine zipper (LZ) motif and a C-terminal WRKY domain. RPS4 and RRS1 are localised near each other in a head-to-head orientation. In this study, direct mutagenesis of the C-terminal LZ motif in RRS1 caused an autoimmune response and stunting in the mutant. Co-immunoprecipitation analysis indicated that full-length RPS4 and RRS1 are physically associated with one another. Furthermore, virus-induced gene silencing experiments showed that hypersensitive-like cell death triggered by RPS4/LZ motif-mutated RRS1 depends on EDS1. In conclusion, we suggest that the RRS1-LZ motif is crucial for the regulation of the RPS4/RRS1 complex.
authors.affiliation: Research Institute for Biological Sciences Okayama, Okayama 716-1241, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.affiliation: Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Toyoda
authors.affiliation: Research Institute for Biological Sciences Okayama, Okayama 716-1241, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Shiraishi
authors.affiliation: RIKEN BioResource Centre, Tsukuba 305-0074, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iuchi
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Takano
authors.affiliation: RIKEN Centre for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Shirasu
authors.affiliation: Research Institute for Biological Sciences Okayama, Okayama 716-1241, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Narusaka
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000276:immunology; D020231:Colletotrichum / Q000254:growth & development; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D016350:Leucine Zippers; D009154:Mutation; D017860:Nepovirus / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D011485:Protein Binding; D000072417:Protein Domains; D044224:Pseudomonas syringae / Q000254:growth & development; D043368:Ralstonia solanacearum / Q000254:growth & development; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; D010940:Plant Proteins; C582898:RRS1 protein, Arabidopsis; C097421:rps4 protein, plant