title: Adaptation of the fish juvenile growth test (OECD TG 215, 2000) to the marine species Dicentrarchus labrax.
abstract: The OECD TG 215 method (2000) (C.14 method of EC Regulation 440/2008) was developed on the rainbow trout (Oncorynchus mykiss) to assess chronic toxicity (28d) of chemicals on fish juveniles. It contemplates to use other well documented species identifying suitable conditions to evaluate their growth. OECD proposes the European sea bass (Dicentrarchus labrax, L. 1758) as Mediterranean species among vertebrates recommended in the OECD guidelines for the toxicity testing of chemicals. In this context, our study is aimed to proposing the adaptation of the growth test (OECD TG 215, 2000) to D. labrax. For this purpose toxicity tests were performed with sodium dodecyl sulfate, a reference toxicant commonly used in fish toxicity assays. The main aspects of the testing procedure were reviewed: fish size (weight), environmental conditions, dilution water type, experimental design, loading rate and stocking density, feeding (food type and ration), test validity criteria. The experience gained from growth tests with the sea bass allows to promote its inclusion among the species to be used for the C.14 method.
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy. Electronic address: andrea.tornambe@isprambiente.it.
authors.forename: A
authors.initials: A
authors.lastname: Tornambè
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Manfra
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Canepa
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Oteri
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Martuccio
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Cicero
authors.affiliation: ISPRA, Italian Institute for Environmental Protection and Research, via Vitaliano Brancati 48, 00144 Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Magaletti
mesh_terms: D000818:Animals; D001492:Bass / Q000254:growth & development*; D001835:Body Weight; D066150:Organisation for Economic Co-Operation and Development; D012107:Research Design / Q000331:legislation & jurisprudence / Q000639:trends; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*; D013045:Species Specificity; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D012967:Sodium Dodecyl Sulfate
keywords: Dicentrarchus labrax; Fish growth toxicity test; OECD 215; Sodium dodecyl sulfate