title: Essential RNA-Based Technologies and Their Applications in Plant Functional Genomics.
abstract: Genome sequencing has not only extended our understanding of the blueprints of many plant species but has also revealed the secrets of coding and non-coding genes. We present here a brief introduction to and personal account of key RNA-based technologies, as well as their development and applications for functional genomics of plant coding and non-coding genes, with a focus on short tandem target mimics (STTMs), artificial microRNAs (amiRNAs), and CRISPR/Cas9. In addition, their use in multiplex technologies for the functional dissection of gene networks is discussed.
authors.affiliation: Provincial State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; School of Biotechnology, Gautam Buddha University, Greater Noida, UP 201312, India; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
authors.forename: Sachin
authors.initials: S
authors.lastname: Teotia
authors.affiliation: School of Biotechnology, Gautam Buddha University, Greater Noida, UP 201312, India; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
authors.forename: Deepali
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Tang
authors.affiliation: Provincial State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA. Electronic address: gtang1@mtu.edu.
authors.forename: Guiliang
authors.initials: G
authors.lastname: Tang
mesh_terms: D053263:Gene Regulatory Networks; D020868:Gene Silencing; D018390:Gene Targeting; D017343:Genes, Plant; D023281:Genomics / Q000379:methods*; D018521:Plant Physiological Phenomena; D010944:Plants / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*
chemical_list: D018749:RNA, Plant
keywords: CRISPR/Cas9; artificial microRNA (amiRNA); artificial/synthetic tasiRNA (atasiRNA/syn-tasiRNA); microRNA sponge (SP); short tandem target mimic (STTM); target mimic (TM)