title: Investigation on the interaction between isorhamnetin and bovine liver catalase by spectroscopic techniques under different pH conditions.
abstract: The binding of isorhamnetin to bovine liver catalase (BLC) was first investigated at 302, 310 and 318 K at pH 7.4 using spectroscopic methods including fluorescence spectra, circular dichroism (CD) and UV-vis absorption. Spectrophotometric observations are rationalized mainly in terms of a static quenching process. The binding constants and binding sites were evaluated by fluorescence quenching methods. Enzymatic activity of BLC in the absence and presence of isorhamnetin was measured using a UV/vis spectrophotometer. The result revealed that the binding of isorhamnetin to BLC led to a reduction in the activity of BLC. The positive entropy change and enthalpy change indicated that the interaction of isorhamnetin with BLC was mainly driven by hydrophobic forces. The distance r between the donor (BLC) and acceptor (isorhamnetin) was estimated to be 2.99 nm according to fluorescence resonance energy transfer. Fluorescence, synchronous fluorescence, and CD spectra showed no obvious change in the conformation of BLC upon the binding of isorhamnetin. In addition, the influence of pH on the binding of isorhamnetin to BLC was investigated and the binding ability of the drug to BLC deceased under other pH conditions (pH 9.0, 6.5, 5.0, 3.5, or 2.0) as compared with that at pH 7.4. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Life Science, Luoyang Normal University, Luoyang, 471022, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Chemistry & Chemical Engineering, Luoyang Normal University, Luoyang, 471022, China.
authors.forename: Daojin
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000201:enzymology*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: C047368:3-methylquercetin; D011794:Quercetin; D002374:Catalase
keywords: activity of BLC; bovine liver catalase (BLC); circular dichroism (CD); fluorescence quenching; isorhamnetin