title: Differential Expression of Proteins Associated with the Hair Follicle Cycle - Proteomics and Bioinformatics Analyses.
abstract: Hair follicle cycling can be divided into the following three stages: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still unknown. To better understand the detailed protein networks controlling this process, proteomics and bioinformatics analyses were performed to construct comparative protein profiles of mouse skin at specific time points (0, 8, and 20 days). Ninety-five differentially expressed protein spots were identified by MALDI-TOF/TOF as 44 proteins, which were found to change during hair follicle cycle transition. Proteomics analysis revealed that these changes in protein expression are involved in Ca2+-regulated biological processes, migration, and regulation of signal transduction, among other processes. Subsequently, three proteins were selected to validate the reliability of expression patterns using western blotting. Cluster analysis revealed three expression patterns, and each pattern correlated with specific cell processes that occur during the hair cycle. Furthermore, bioinformatics analysis indicated that the differentially expressed proteins impacted multiple biological networks, after which detailed functional analyses were performed. Taken together, the above data may provide insight into the three stages of mouse hair follicle morphogenesis and provide a solid basis for potential therapeutic molecular targets for this hair disease.
authors.affiliation: Department of Dermatology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, China.|Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Wenrong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Dermatology, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, Jiangsu, China.|Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Mifang
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Dermatology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Science, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, China.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Ping
authors.affiliation: Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Fan
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D016000:Cluster Analysis; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006197:Hair / Q000378:metabolism / Q000502:physiology; D018859:Hair Follicle / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D020543:Proteome; D040901:Proteomics; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D002118:Calcium