title: Elevated mRNA expression of PGF2α receptor splice variant 2(FP-V2) in human decidua is associated with incomplete mifepristone-misoprostol-induced early medical abortion by regulation of interleukin-8.
abstract: OBJECTIVE
  The combination of mifepristone and misoprostol is an established method for the induction of early abortion, but 15% of women still experience the unpleasant side effect of incomplete medical abortion. The purpose of this study was to determine whether prostaglandin (PG) F2α receptor (FP) and its two isoforms (FP-V1 and FP-V2) in human decidua are associated incomplete abortion.
  
  
  METHODS
  Forty women who underwent medical abortion were recruited. Among them, there were 20 cases of incomplete abortion. The other 20 cases of complete abortion were used as controls. The expression levels of FP, FPV1 and FP-V2 in the decidua between of the two groups was detected by quantitative real-time polymerase chain reaction (PCR). Additionally, FP-V2 was knocked down using specific small interfering RNAs (siRNAs) in the primary cultures of decidual cells. The expression levels of cytokines in FP-V2 knockdown primary decidual cells and control decidual cells were detected by quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The FP and FP-V2 mRNA expression in the incomplete group was significantly higher than that in the complete group (p < 0.05). IL-8 was up-regulated by FP-V2 knockdown in primary-cultured decidual cells (p < 0.05).
  
  
  CONCLUSIONS
  These results suggested that the elevated expression of FP-V2 in human decidua is significantly associated with incomplete mifepristone-misoprostol-induced early medical abortion and that IL-8 could be lined to this process.
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Feng
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Weitian
authors.initials: W
authors.lastname: Han
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Lu
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sui
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhao
authors.affiliation: b Department of Physiology , Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada, University of Toronto , Toronto , Canada.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lye
authors.affiliation: a Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Liaoning Province Research Institute of Family Planning , Shenyang , China and.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Li
mesh_terms: D000021:Abortifacient Agents, Steroidal / Q000494:pharmacology; D000028:Abortion, Induced / Q000009:adverse effects*; D000328:Adult; D000704:Analysis of Variance; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D003656:Decidua / Q000378:metabolism*; D015237:Dinoprost / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D015735:Mifepristone / Q000494:pharmacology; D016595:Misoprostol / Q000494:pharmacology; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000021:Abortifacient Agents, Steroidal; D016207:Cytokines; D016209:Interleukin-8; D020033:Protein Isoforms; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D016595:Misoprostol; D015735:Mifepristone; D015237:Dinoprost
keywords: Cytokines; isoforms; pregnancy; prostaglandin receptors; sensitivity