title: Dioscorea bulbifera polysaccharide and cyclophosphamide combination enhances anti-cervical cancer effect and attenuates immunosuppression and oxidative stress in mice.
abstract: Cyclophosphamide (CTX) is commonly used in cancer chemotherapy, which causes immunosuppression and tissue oxidative stress at high doses. As potential protective agents, some polysaccharides were shown to have anti-tumor, anti-inflammatory and/or anti-oxidant properties. This study explored potential effects of oral treatment of Dioscorea bulbifera polysaccharides (DBLP at 100 or 150 mg/kg) in U14 cervical tumor-bearing mice treated with CTX (25 mg/kg). While CTX suppressed tumor growth (65.4% inhibition) and DBLP alone also inhibited tumor (25.6% at 100 mg/kg or 37.6% at 150 mg/kg), CTX+DBLP combination produced tumor inhibition rates of 5.6 (for 100 mg/kg DBLP) or 9% (for 150 mg/kg) higher than CTX alone. While tumor itself and CTX treatment reduced thymus and/or spleen/body weight indices, DBLP alone or CTX + DBLP combination attenuated this reduction. DBLP lowered peripheral blood T-cell subpopulation CD(4+)/CD(8+) ratio, and DBLP+CTX combination attenuated CTX effect in lifting CD(4+)/CD(8+) ratio. Tumor itself and CTX treatment heightened oxidative stress (with decreased superoxide dismutase but increased lactate dehydrogenase and malondialdehyde levels in serum and tissues), which was attenuated by DBLP treatment, and DBLP+CTX combination suppressed CTX-induced oxidative stress. Combination use of DBLP with CTX can potentially enhance CTX anti-tumor effect and can attenuate CTX-induced immunosuppression and oxidative stress in U14 cervical tumor-bearing mice.
authors.affiliation: College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, China.|Hebei Province Key Laboratory of Applied Chemistry, Qinhuangdao, Hebei 066004, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Sansom Institute for Health Research and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Sansom Institute for Health Research and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Xian
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D003520:Cyclophosphamide / Q000494:pharmacology*; D027742:Dioscorea / Q000737:chemistry*; D004195:Disease Models, Animal; D004357:Drug Synergism; D005260:Female; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018655:Lymphocyte Count; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D010936:Plant Extracts; D011134:Polysaccharides; D008315:Malondialdehyde; D003520:Cyclophosphamide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase