title: Assessment of glutathione S-transferaseM1 (GSTM1) and its polymorphisms GSTM1 null in the response to treatment with chemotherapy in advanced ovarian carcinoma.
abstract: OBJECTIVE
  To assess if the genotype of the glutathione S-transferase M1 (GSTM1) enzyme and its GSTM1 null polymorphism can influence the response to chemotherapeutic treatment of advanced ovarian cancer.
  
  
  METHODS
  Case-control study of 112 patients with advanced ovarian cancer submitted to chemotherapy during the period from January 1995 to December 2005. The tissue to study the GSTM1 genotype and its deletion came from surgical staging to treat ovarian cancer. The PCR product generates two distinct genotypes, characterized as positive and null. The response to chemotherapy was evaluated using the World Health Organization (WHO) criteria. Patients were classified as having: a) no response, b) a response.
  
  
  RESULTS
  The presence of GSTM1 or its GSTM1 null polymorphism did not influence the preoperative chemotherapy response. Among the patients who did respond, 88.9% presented with positive GSTM1 and 11.1% with null GSTM1. Among the patients that did not respond, 85.71% presented with positive GSTM1 and 14.29% with null GSTM1 (p = 0.825). GSTM1 and its GSTM1 null polymorphism had no influence on the postoperative response to chemotherapy. Among the patients who did respond, 80.65% presented with positive GSTM1 and 19.35% with null GSTM1. Among the patients who did not respond, 87.50% presented with positive GSTM1 and 12.5% with the null polymorphism (p = 0.553).
  
  
  CONCLUSIONS
  No difference was observed in the response to treatment with chemotherapy in patients with advanced ovarian cancer, as to the GSTM1 genotype compared to its GSTM1 null polymorphism.
authors.affiliation: Department of Gynecology and Obstetrics, Hospital Servidor Público Estadual "Francisco Morato de Oliveira" - HSPE-FMO, Sao Paulo, SP, BR.
authors.forename: Gilberto Kendi
authors.initials: GK
authors.lastname: Takeda
authors.affiliation: Molecular Gynecology Laboratory, Department of Gynecology, Escola Paulista de Medicina, Universidade Federal de São Paulo - UNIFESP, São Paulo, SP, BR.
authors.forename: Daniela Batista
authors.initials: DB
authors.lastname: Leite
authors.affiliation: Molecular Gynecology Laboratory, Department of Gynecology, Escola Paulista de Medicina, Universidade Federal de São Paulo - UNIFESP, São Paulo, SP, BR.
authors.forename: Michele Gilvana
authors.initials: MG
authors.lastname: Junqueira
authors.affiliation: Department of Pelvic Oncology, Hospital do Servidor Público Estadual "Francisco Morato de Oliveira" - UNIFESP, Sao Paulo, SP, BR.
authors.forename: Luiz Augusto Freire
authors.initials: LA
authors.lastname: Lopes
authors.affiliation: Department of Gynecology, Escola Paulista de Medicina, Universidade Federal de São Paulo - UNIFESP, Sao Paulo, SP, BR.
authors.forename: Ismael Dale Cotrim Guerreiro da
authors.initials: ID
authors.lastname: Silva
authors.affiliation: Department of Gynecology, Hospital do Servidor Público Estadual "Francisco Morato de Oliveira" - HSPE-FMO, Sao Paulo, SP, BR.
authors.forename: Fausto Farah
authors.initials: FF
authors.lastname: Baracat