title: Association of PNPLA3 Polymorphism with Hepatocellular Carcinoma Development and Prognosis in Viral and Non-Viral Chronic Liver Diseases.
abstract: BACKGROUND
  The aim of this study was to evaluate any association between a single nucleotide polymorphism (SNP) in the patatin-like phospholipase domain containing 3 (PNPLA3) (rs738409, C>G) and the development and prognosis in patients with hepatocellular carcinoma (HCC).
  
  
  METHODS
  Two hundred heathy controls and 388 HCC cases were included: 211 with HBV, 98 patients with HCV, 29 with alcoholic steatohepatitis (ASH) and 52 with non-alcoholic steatohepatitis (NASH). The SNP was determined by real-time PCR based on TaqMan assays.
  
  
  RESULTS
  The prevalence of rs738409 genotypes CC, CG and GG in controls was 91 (45.5%), 88 (44.0%), and 21 (10.5%), respectively, while the corresponding genotypes in all patients with HCC was 158 (40.7%), 178 (45.9%), and 52 (13.4%). The GG genotype had significantly higher distribution in patients with ASH/NASH-related HCC compared with controls (OR=2.34, 95% CI=1.16-4.71, P=0.018), and viral-related HCC cases (OR=2.15, 95% CI=1.13-4.08, P=0.020). However, the frequency of the GG genotype was similar between controls and patients with viral-related HCC. At initial diagnosis, HBV-related HCC were larger and at more advanced BCLC stage than the other HCC groups. There were no significant differences between the GG and non-GG groups regarding clinical characteristics, tumor stage and overall survival.
  
  
  CONCLUSIONS
  These data suggest an influence of the PNPLA3 polymorphism on the occurrence of HCC in patients with ASH/NASH but not among those with chronic viral hepatitis. However, the polymorphism was not associated with the prognosis of HCC.
authors.affiliation: Department of Biochemistry Research Unit of Hepatitis and Liver Cancer, Chulalongkorn University, Bangkok, Thailand E-mail : pisittkvn@yahoo.com.
authors.forename: Apichaya
authors.initials: A
authors.lastname: Khlaiphuengsin
authors.forename: Rattanaporn
authors.initials: R
authors.lastname: Kiatbumrung
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Nutcha
authors.initials: N
authors.lastname: Pinjaroen
authors.forename: Pisit
authors.initials: P
authors.lastname: Tangkijvanich
mesh_terms: D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology; D016022:Case-Control Studies; D018450:Disease Progression; D005235:Fatty Liver, Alcoholic / Q000235:genetics* / Q000473:pathology / Q000821:virology; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006521:Hepatitis, Chronic / Q000235:genetics* / Q000473:pathology / Q000821:virology; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics* / Q000473:pathology / Q000821:virology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D012307:Risk Factors; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
chemical_list: D008049:Lipase; D008565:Membrane Proteins; C533682:PNPLA3 protein, human; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent