title: miR-421 is a diagnostic and prognostic marker in patients with osteosarcoma.
abstract: MicroRNAs (miRNAs) are a new class of prognostic and diagnostic biomarkers for many cancers. Recent studies have shown that miRNAs are highly stable in plasma/serum. The aim of this study was to investigate the role of miR-421 in osteosarcoma. We found that the serum expression levels of miR-421 were significantly higher in osteosarcoma patients than those in healthy volunteers. Moreover, miR-421 expression was significantly higher in osteosarcoma tissues compared with that in the adjacent normal tissues. Meanwhile, the expression of miR-421 was upregulated in 90 % (36/40) osteosarcoma tissues compared to non-tumor tissues. More importantly, the expression levels of miR-421 in osteosarcoma tissues were correlated with those in patients' serum. In addition, patients with high miR-421 expression had shorter overall survival (OS) than those with low expressions. We also found that overexpression of miR-421 promoted osteosarcoma cell line MG-63 proliferation, migration, and invasion. In conclusion, miR-421 expression levels were upregulated in osteosarcoma tissue and serum and it may be a useful marker for diagnosis of osteosarcoma.
authors.affiliation: Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.|Department of Orthopedics, The Affiliated Hospital of Hunan University of Medicine and The Third People's Hospital of Huaihua, Huaihua, 418000, Hunan, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. wang11bin1@sina.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.|Department of Orthopedics, The First Hospital of Kunming, Kunming, 650011, Yunnan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.|Department of Orthopedics, The People's Hospital of Qingyuan, Qingyuan, 511518, Guangdong, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Hunan University of Medicine, Huaihua, 418000, Hunan, China.
authors.forename: Mengzhen
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Hunan University of Medicine and The Third People's Hospital of Huaihua, Huaihua, 418000, Hunan, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Hunan University of Medicine and The Third People's Hospital of Huaihua, Huaihua, 418000, Hunan, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Spine Surgery, Yan'an Hospital of Kunming, Kunming, 650051, Yunnan, China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Yang
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001859:Bone Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D012516:Osteosarcoma / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C547665:MIRN421 microRNA, human; D035683:MicroRNAs
keywords: Invasion; MicroRNAs; Osteosarcoma; Serum miRNA; miR-421