title: The tyrosine-sorting motif of the vacuolar sorting receptor VSR4 from Arabidopsis thaliana, which is involved in the interaction between VSR4 and AP1M2, μ1-adaptin type 2 of clathrin adaptor complex 1 subunits, participates in the post-Golgi sorting of VSR4.
abstract: μ1-Adaptin of adaptor protein (AP) 1 complex, AP1M, is generally accepted to load cargo proteins into clathrin-coated vesicles (CCVs) at the trans-Golgi network through its binding to cargo-recognition sequences (CRSs). Plant vacuolar-sorting receptors (VSRs) function in sorting vacuolar proteins, which are reportedly mediated by CCV. We herein investigated the involvement of CRSs of Arabidopsis thaliana VSR4 in the sorting of VSR4. The results obtained showed the increased localization of VSR4 at the plasma membrane or vacuoles by mutations in CRSs including the tyrosine-sorting motif YMPL or acidic dileucine-like motif EIRAIM, respectively. Interaction analysis using the bimolecular fluorescence complementation (BiFC) system, V10-BiFC, which we developed, indicated an interaction between VSR4 and AP1M2, AP1M type 2, which was attenuated by a YMPL mutation, but not influenced by an EIRAIM mutation. These results demonstrated the significance of the recognition of YMPL in VSR4 by AP1M2 for the post-Golgi sorting of VSR4.
authors.affiliation: a Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization of Research , Shimane University , Matsue , Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishimura
authors.affiliation: b Department of Life Science and Biotechnology , Shimane University , Matsue , Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Matsunami
authors.affiliation: b Department of Life Science and Biotechnology , Shimane University , Matsue , Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: b Department of Life Science and Biotechnology , Shimane University , Matsue , Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Kohata
authors.affiliation: c Department of Pharmacology , National Research Institute for Child Health and Development , Tokyo , Japan.|d Graduate School of Medical and Dental Sciences , Tokyo Medical and Dental University , Tokyo , Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Yamauchi
authors.affiliation: b Department of Life Science and Biotechnology , Shimane University , Matsue , Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Jisaka
authors.affiliation: b Department of Life Science and Biotechnology , Shimane University , Matsue , Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagaya
authors.affiliation: b Department of Life Science and Biotechnology , Shimane University , Matsue , Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yokota
authors.affiliation: a Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization of Research , Shimane University , Matsue , Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: C583122:AP1M2 protein, Arabidopsis; D033961:Adaptor Protein Complex 1; D029681:Arabidopsis Proteins; D014443:Tyrosine
keywords: Arabidopsis thaliana; BiFC; VSR4; clathrin; tyrosine-sorting motif