title: DPSC colonization of functionalized 3D textiles.
abstract: Fiber scaffolds are attractive materials for mimicking, within a 3D in vitro system, any living environment in which animal cells can adhere and proliferate. In three dimensions, cells have the ability to communicate and organize into complex architectures similar to those found in their natural environments. The aim of this study was to evaluate, in terms of cell reactivity, a new in vitro cell model: dental pulp stem cells (DPSCs) in a 3D polymeric textile. Scaffolds were knitted from polyglycolic acid (PGA) or polydioxanone (PDO) fibers differing in surface roughness. To promote cell adhesion, these hydrophobic fabrics were also functionalized with either chitosan or the peptide arginine-glycine-aspartic acid (RGD). Cell behavior was examined 1, 10, and 21 days post-seeding with a LIVE/DEAD®Kit. Confocal laser scanning microscopy (CLSM) highlighted the biocompatibility of these materials (cell survival rate: 94% to 100%). Fiber roughness was found to influence cell adhesion and viability significantly and favorably. A clear benefit of polymeric textile functionalization with chitosan or RGD was demonstrated in terms of cell adhesion and viability. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 785-794, 2017.
authors.affiliation: Institutional Doctorate in Engineering and Science Materials, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.|Laboratory of Basic Science, Faculty of Dentistry, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.
authors.forename: Marine
authors.initials: M
authors.lastname: Ortiz
authors.affiliation: Laboratory of Basic Science, Faculty of Dentistry, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rosales-Ibáñez
authors.affiliation: Institutional Doctorate in Engineering and Science Materials, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.|Laboratory of Basic Science, Faculty of Dentistry, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.
authors.forename: Amaury
authors.initials: A
authors.lastname: Pozos-Guillén
authors.affiliation: Laboratoire de Génie chimique, University of Liège, Chemistry Institute, Liège, Belgique.
authors.forename: Charlotte
authors.initials: C
authors.lastname: De Bien
authors.affiliation: Laboratoire de Génie chimique, University of Liège, Chemistry Institute, Liège, Belgique.
authors.forename: Dominique
authors.initials: D
authors.lastname: Toye
authors.affiliation: Institutional Doctorate in Engineering and Science Materials, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.|Laboratory of Basic Science, Faculty of Dentistry, Universidad Autónoma de San Luis Potosí, S.L.P., Mexico.
authors.forename: Héctor
authors.initials: H
authors.lastname: Flores
authors.affiliation: Interfacultary Center of Biomaterials, Université de Liège, Chemistry Institute, Liège, Belgique.
authors.forename: Christian
authors.initials: C
authors.lastname: Grandfils
mesh_terms: D000293:Adolescent; D000328:Adult; D002448:Cell Adhesion; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D009842:Oligopeptides / Q000737:chemistry; D016687:Polydioxanone / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013784:Textiles; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D011100:Polyglycolic Acid; D016687:Polydioxanone; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan
keywords: biocompatibility; biomimetic scaffold; dental pulp stem cells; in vitro cell model; textile