title: Exposure to sixty minutes of hyperoxia upregulates myocardial humanins in patients with coronary artery disease - a pilot study.
abstract: In experimental setting the concept of myocardial preconditioning by hyperoxia has been introduced and different intracellular protective mechanisms and their effects have been described. To study whether similar protective phenotype can be induced by hyperoxia also in humans, gene expression profile after hyperoxic exposure was analyzed. Adult patients were randomized to be ventilated with either FiO2 0.4 (n = 14) or 1.0 (n = 10) for 60 minutes before coronary artery bypass grafting. A tissue sample from the right atrial appendage was taken for gene analysis and expression profile analysis on genome wide level by RNA-seq analysis was applied. Exposure to > 96% oxygen for 60 minutes significantly changed the expression of 20 different genes, including upregulation of two different humanins - MTRNR2L2 and MTRNR2L8, and activated a "cell survival" network as detected by Ingenuity Pathway Analyses. We concluded that administration of > 96% oxygen for 1 hour changes gene expression in the myocardium of the patients with coronary artery disease and may enhance cell survival capability.
authors.affiliation: Clinic of Anesthesiology and Intensive Care, North Estonia Medical Centre, Tallinn, Estonia. inga.karu@regionaalhaigla.ee.|Department of Anaesthesiology and Intensive Care, University of Tartu, Tartu, Estonia.
authors.forename: I
authors.initials: I
authors.lastname: Karu
authors.affiliation: Department of Anaesthesiology and Intensive Care, University of Tartu, Tartu, Estonia.|Department of Cardiac Surgery, Tartu University Hospital, Tartu, Estonia.
authors.forename: P
authors.initials: P
authors.lastname: Tahepold
authors.affiliation: Department of Cardiac Surgery, Tartu University Hospital, Tartu, Estonia.
authors.forename: A
authors.initials: A
authors.lastname: Ruusalepp
authors.affiliation: Department of Pathophysiology, University of Tartu, Tartu, Estonia.
authors.forename: E
authors.initials: E
authors.lastname: Reimann
authors.affiliation: Department of Pathophysiology, University of Tartu, Tartu, Estonia.
authors.forename: S
authors.initials: S
authors.lastname: Koks
authors.affiliation: Department of Anaesthesiology and Intensive Care, University of Tartu, Tartu, Estonia.
authors.forename: J
authors.initials: J
authors.lastname: Starkopf
mesh_terms: D000368:Aged; D003324:Coronary Artery Disease / Q000235:genetics / Q000628:therapy*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000627:therapeutic use*; D010865:Pilot Projects; D015854:Up-Regulation
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C427211:humanin; D010100:Oxygen