title: Multiplex cytokine levels of aqueous humor in acute primary angle-closure patients: fellow eye comparison.
abstract: BACKGROUND
  The existing literature contains no information regarding inflammatory cytokine expression in unilateral acute primary angle-closure (APAC) affected eyes and fellow eyes with primary angle closure suspect (PACS). To measure levels of various inflammatory cytokines in the aqueous humor (AH) of APAC affected eyes and fellow eyes with a diagnosis of PACS (18 unilateral APAC eyes and 18 fellow eyes with PACS), and determine the underlying correlation between them.
  
  
  METHODS
  The total levels of 12 cytokines including granulocyte colony-stimulating factor (G-CSF), interleukin (IL)-6, IL-8, monocyte chemotactic protein (MCP)-1, MCP-3, macrophage-derived chemokine (MDC), macrophage inflammatory protein (MIP)-1β, and vascular endothelial growth factor (VEGF) etc. were assessed using the multiplex bead immunoassay technique. The level of cytokines in different groups was analyzed by a 2-related-samples nonparametric test. Data on patient demographics, preoperative intraocular pressure (IOP), number of glaucoma medications, as well as several ocular biological parameters were also collected for correlation analysis.
  
  
  RESULTS
  The APAC patients had significantly higher levels of G-CSF, IL-6, IL-8, MCP-1, MCP-3, MDC, MIP-1β, and VEGF in the AH samples from unilateral APAC affected eyes than in fellow eyes with PACS (all P < 0.05). The cytokines showed positive correlations between each other (P < 0.0071).
  
  
  CONCLUSIONS
  Cytokine networks in the AH may have critical roles in the progression of APAC. Thus, different cytokine expression in both eyes of the same patient may help us to understand the different pathology in APAC and PACS.
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.|Tungwah Hospital of Sun Yat-Sen University, Dongguan, China.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Du
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China. zhangxl2@mail.sysu.edu.cn.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China. dlam.sklo.sysu.cn@gmail.com.
authors.forename: Dennis S C
authors.initials: DSC
authors.lastname: Lam
mesh_terms: D000208:Acute Disease; D000368:Aged; D000959:Antihypertensive Agents / Q000627:therapeutic use; D001082:Aqueous Humor / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D015812:Glaucoma, Angle-Closure / Q000175:diagnosis / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D007429:Intraocular Pressure / Q000187:drug effects; D008297:Male; D033401:Microscopy, Acoustic; D008875:Middle Aged; D014065:Tonometry, Ocular
chemical_list: D000959:Antihypertensive Agents; D016207:Cytokines