title: Antiviral Activity of a Cloned Peptide RC28 Isolated from the Higher Basidiomycetes Mushroom Rozites caperata in a Mouse Model of HSV-1 Keratitis.
abstract: An Escherichia coli-expressed peptide with a molecular weight of 28.26, derived from the complementary DNA of antiviral protein RC28 isolated from the mushroom Rozites caperata (=Cortinarius caperatus), demonstrated potent antiviral activity against herpes simplex virus-1 in Vero cells and in a herpes simplex virus-1 mouse keratitis model. Plaque assays in Vero cells showed that the peptide reduced viral yields by at least 1.2 logs; in the animal model the cloned peptide delayed the occurrence of stromal keratitis and alleviated the severity of the disease. We believe this is the first report of a cloned mushroom peptide with antiviral activity for the prevention and treatment of a viral disease.
authors.affiliation: Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Naihong
authors.initials: N
authors.lastname: Yan
authors.affiliation: Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Fen
authors.initials: F
authors.lastname: He
authors.affiliation: Department of Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Frank F
authors.initials: FF
authors.lastname: Piraino
authors.affiliation: Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Haotian
authors.initials: H
authors.lastname: Xiang
authors.affiliation: Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000363:Agaricales; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001487:Basidiomycota / Q000737:chemistry*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003316:Corneal Diseases / Q000821:virology*; D003319:Corneal Stroma / Q000187:drug effects* / Q000821:virology; D018076:DNA, Complementary; D004195:Disease Models, Animal; D004926:Escherichia coli; D005260:Female; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development; D016849:Keratitis, Herpetic / Q000188:drug therapy* / Q000821:virology; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D012720:Severity of Illness Index; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D018076:DNA, Complementary; D010455:Peptides