title: [Radioligand Method of Assessment of β-Adrenoceptor's Activity on Human T-Lymphocytes].
abstract: We proposed a new method of evaluation of beta-receptor's activity on the surface of human T-lymphocytes based on the radioligand method. Optimal conditions for evaluation of specific binding to β2-adrenoceptors of 0.5 fmol ligand per 1 million cells using [125I]-cyanopindolol were found. The possibility of using of β2-adrenoceptor's activity assessment in clinical settings was demonstrated on human T-lymphocyte.
authors.forename: O Y
authors.initials: OY
authors.lastname: Agapova
authors.forename: Y S
authors.initials: YS
authors.lastname: Skoblov
authors.forename: K A
authors.initials: KA
authors.lastname: Zykov
authors.forename: A V
authors.initials: AV
authors.lastname: Rvacheva
authors.forename: V B
authors.initials: VB
authors.lastname: Beilina
authors.forename: V P
authors.initials: VP
authors.lastname: Masenko
authors.forename: I E
authors.initials: IE
authors.lastname: Chazova
mesh_terms: D000319:Adrenergic beta-Antagonists; D002478:Cells, Cultured; D006801:Humans; D007457:Iodine Radioisotopes; D008024:Ligands; D010869:Pindolol / Q000031:analogs & derivatives*; D011485:Protein Binding; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000319:Adrenergic beta-Antagonists; D007457:Iodine Radioisotopes; D008024:Ligands; D011943:Receptors, Adrenergic, beta; C047975:cyanopindolol; D010869:Pindolol