title: Variable protection against experimental broiler necrotic enteritis after immunization with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant.
abstract: Necrotic enteritis toxin B (NetB) is a pore-forming toxin produced by Clostridium perfringens and has been shown to play a key role in avian necrotic enteritis, a disease causing significant costs to the poultry production industry worldwide. The aim of this work was to determine whether immunization with a non-toxic variant of NetB (NetB W262A) and the C-terminal fragment of C. perfringens alpha-toxin (CPA247-370) would provide protection against experimental necrotic enteritis. Immunized birds with either antigen or a combination of antigens developed serum antibody levels against NetB and CPA. When CPA247-370 and NetB W262A were used in combination as immunogens, an increased protection was observed after oral challenge by individual dosing, but not after in-feed-challenge.
authors.affiliation: a College of Life and Environmental Sciences , University of Exeter , Exeter , UK.
authors.forename: Sérgio P
authors.initials: SP
authors.lastname: Fernandes da Costa
authors.affiliation: b Faculty of Veterinary Medicine, Department of Pathology, Bacteriology and Avian Diseases , Ghent University , Merelbeke , Belgium.
authors.forename: Dorien
authors.initials: D
authors.lastname: Mot
authors.affiliation: b Faculty of Veterinary Medicine, Department of Pathology, Bacteriology and Avian Diseases , Ghent University , Merelbeke , Belgium.
authors.forename: Sofie
authors.initials: S
authors.lastname: Geeraerts
authors.affiliation: a College of Life and Environmental Sciences , University of Exeter , Exeter , UK.
authors.forename: Monika
authors.initials: M
authors.lastname: Bokori-Brown
authors.affiliation: b Faculty of Veterinary Medicine, Department of Pathology, Bacteriology and Avian Diseases , Ghent University , Merelbeke , Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Van Immerseel
authors.affiliation: a College of Life and Environmental Sciences , University of Exeter , Exeter , UK.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001530:Belgium; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D003015:Clostridium Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D003016:Clostridium perfringens / Q000276:immunology*; D004751:Enteritis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000662:veterinary; D008297:Male; D009336:Necrosis / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D010738:Type C Phospholipases / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D004768:Enterotoxins; C534774:NetB protein, Clostridium perfringens; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens
keywords: Clostridium perfringens; NetB; alpha-toxin; immunization; necrotic enteritis