title: The AP-2 complex is required for proper temporal and spatial dynamics of endocytic patches in fission yeast.
abstract: In metazoans the AP-2 complex has a well-defined role in clathrin-mediated endocytosis. By contrast, its direct role in endocytosis in unicellular eukaryotes has been questioned. Here, we report co- immunoprecipitation between the fission yeast AP-2 component Apl3p and clathrin, as well as the genetic interactions between apl3Δ and clc1 and sla2Δ/end4Δ mutants. Furthermore, a double clc1 apl3Δ mutant was found to be defective in FM4-64 uptake. In an otherwise wild-type strain, apl3Δ cells exhibit altered dynamics of the endocytic sites, with a heterogeneous and extended lifetime of early and late markers at the patches. Additionally, around 50% of the endocytic patches exhibit abnormal spatial dynamics, with immobile patches and patches that bounce backwards to the cell surface, showing a pervasive effect of the absence of AP-2. These alterations in the endocytic machinery result in abnormal cell wall synthesis and morphogenesis. Our results complement those found in budding yeast and confirm that a direct role of AP-2 in endocytosis has been conserved throughout evolution.
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: Nagore
authors.initials: N
authors.lastname: de León
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Hoya
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: M-Angeles
authors.initials: MA
authors.lastname: Curto
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Moro
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Yanguas
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Doncel
authors.affiliation: Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), University of Salamanca/CSIC, Calle Zacarías González 2, 37007, Salamanca, Spain.
authors.forename: M-Henar
authors.initials: MH
authors.lastname: Valdivieso
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000235:genetics*; D002473:Cell Wall / Q000378:metabolism; D033941:Clathrin Light Chains / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000235:genetics* / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D033962:Adaptor Protein Complex 2; D033941:Clathrin Light Chains; C587595:Clc1 protein, S pombe; C506561:End4 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; C506560:Sla2 protein, S pombe