title: Basal transcription of APOBEC3G is regulated by USF1 gene in hepatocyte.
abstract: Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G, A3G) exert antiviral defense as an important factor of innate immunity. A variety of cytokines such as IFN-γ、IL2、IL15、IL7 could induce the transcription of A3G. However, the regulation of other nuclear factor on the transcription of A3G have not been reported at the present. To gain new insights into the transcriptional regulation of this restriction factor, we cloned and characterized the promoter region of A3G and investigate the modulation of USF1 gene on the transcription of A3G. We identified a 232 bp region that was sufficient to regulate the activity of full promoter. Transcriptional start sites (TSS) were identified by the luciferase reporter assays of plasmids containing full or shorter fragments of the A3G promoter. The results demonstrated that the core promoter of A3G is located within the region -159/-84 relative to the TSS. Transcriptional activity of A3G core promoter regulated by USF1 was dependent on an E-box (located at position -91/-86 relative to the major TSS) and was abolished after mutation of this DNA element. USF1 gene can take part in basal transcription regulation of the human A3G gene in hepatocyte, and the identified E-box represented a binding site for the USF1.
authors.affiliation: Department of Infectious Diseases, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, 450003, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science Technology, Wuhan, 430000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, 450003, China.
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, 450003, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Shang
authors.affiliation: Department of Infectious Diseases, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, 450003, China. Electronic address: kykangyi@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Kang
mesh_terms: D000071480:APOBEC-3G Deaminase; D003564:Cytidine Deaminase / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000502:physiology*; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D051720:Upstream Stimulatory Factors / Q000235:genetics*
chemical_list: C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase
keywords: APOBEC3G; Transcriptional start sites (TSS); USF1