title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell.
abstract: Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the (18)O-cholesterol and (15)N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of (18)O-cholesterol and (15)N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the (18)O-enriched regions suggest that these (18)O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, Illinois 61801.
authors.forename: Ashley N
authors.initials: AN
authors.lastname: Yeager
authors.affiliation: Glenn T. Seaborg Institute, Lawrence Livermore National Laboratory, Livermore, California 94551.
authors.forename: Peter K
authors.initials: PK
authors.lastname: Weber
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, Illinois 61801.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Kraft
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000032:analysis*; D004285:Dogs; D021621:Imaging, Three-Dimensional; D007553:Isotope Labeling; D061985:Madin Darby Canine Kidney Cells / Q000737:chemistry*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D013107:Sphingolipids / Q000032:analysis*
chemical_list: D013107:Sphingolipids; D002784:Cholesterol