title: The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery.
abstract: While myeloid-derived suppressor cells (MDSCs) have been reported to participate in the promotion of angiogenesis and tumor growth, little is known about their presence and function during perioperative period. Here, we demonstrated that human MDSCs expressing CD11b(+), CD33(+) and HLA-DR(-) significantly increased in lung cancer patients after thoracotomy. CD11b(+) CD33(+) HLA-DR(-) MDSCs isolated 24 hr after surgery from lung cancer patients were more efficient in promoting angiogenesis and tumor growth than MDSCs isolated before surgical operation in allograft tumor model. In addition, CD11b(+) CD33(+) HLA-DR(-) MDSCs produced high levels of MMP-9. Using an experimental lung metastasis mouse model, we demonstrated that the numbers of metastases on lung surface and Gr-1(+) CD11b(+) MDSCs at postoperative period were enhanced in proportion to the degree of surgical manipulation. We also examined that syngeneic bone marrow mesenchymal stem cells (BMSCs) significantly inhibited the induction and proliferation of Gr-1(+) CD11b(+) MDSCs and further prevented lung metastasis formation in the mice undergoing laparotomy. Taken together, our results suggest that postoperatively induced MDSCs were qualified with potent proangiogenic and tumor-promotive ability and this cell population should be considered as a target for preventing postoperative tumor metastasis.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Biomedical Research Center, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Xiaosan
authors.initials: X
authors.lastname: Su
authors.affiliation: Biomedical Research Center, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Qiao
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Pathology, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.affiliation: Biomedical Research Center, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Biomedical Research Center, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Yiyin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biomedical Research Center, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Biomedical Research Center, The First Hospital of Kunming (the Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Hong
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D051379:Mice; D022423:Myeloid Cells / Q000637:transplantation*; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000628:therapy*; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D039481:CD11b Antigen; C571163:CD33 protein, human; D006684:HLA-DR Antigens; C509295:ITGAM protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3; D020780:Matrix Metalloproteinase 9
keywords: bone marrow mesenchymal stem cells; lung cancer; metastasis; myeloid-derived suppressor cells; perioperative period