title: Omega-3 Polyunsaturated Fatty Acids Inhibited Tumor Growth via Preventing the Decrease of Genomic DNA Methylation in Colorectal Cancer Rats.
abstract: Omge-3 polyunsaturated fatty acids (PUFAs) exhibited significant effect in inhibiting various tumors. However, the mechanisms of its anticancer role have not been fully demonstrated. The declination of 5-methylcytosine (5 mC) was closely associated with poor prognosis of tumors. To explore whether omega-3 PUFAs influences on DNA methylation level in tumors, colorectal cancer (CRC) rat model were constructed using N-methyl phosphite nitrourea and omega-3 PUFAs were fed to part of the rats during tumor induction. The PUFAs contents in the rats of 3 experimental groups were measured using gas chromatography and 5 mC level were detected by liquid chromatography tandem mass spectrometry. The results showed that tumor incidence in omega-3 treated rats was much lower than in CRC model rats, which confirmed significant antitumor role of omega-3 PUFAs. Six PUFA members categorized to omega-3 and omega-6 families were quantified and the ratio of omega-6/omega-3 PUFAs was remarkably lower in omega-3 PUFAs treatment group than in CRC model group. 5 mC content in omega-3 PUFAs treated rats was higher than in CRC model rats, suggesting omega-3 PUFAs promoted 5 mC synthesis. Therefore, omega-3 PUFAs probably inhibited tumor growth via regulating DNA methylation process, which provided a novel anticancer mechanism of omega-3 PUFAs from epigenetic view.
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wen
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Chen
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Ge
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Gao
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Ye
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Chunan
authors.initials: C
authors.lastname: Liu
authors.affiliation: a Analysis Center, Guangdong Medical University , Zhanjiang , China.
authors.forename: Chun
authors.initials: C
authors.lastname: Cai
mesh_terms: D000818:Animals; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D019175:DNA Methylation; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D023281:Genomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D015525:Fatty Acids, Omega-3