title: Mallotus roxburghianus modulates antioxidant responses in pancreas of diabetic rats.
abstract: Mallotus roxburghianus has long been used by Mizo tribal people for the treatment of diabetes. Scientific validation at known doses may provide information about its safety and efficacy. Methanolic leaf extract of M. roxburghianus (MRME 100 and 400mg/kg) was tested in comparison with normal and alloxan diabetic rats for 28 days p.o. in terms of body and pancreatic weight, blood glucose level, antioxidant enzymes, expression of visfatin and PCNA, histopathology and histomorphometric measurements of pancreas. The results were evaluated statistically using ANOVA, correlation and regression and Principal component analysis (PCO). MRME (100 and 400mg/kg) treatment significantly (p<0.0001) decreased the body weight, blood glucose level, improved the mass and size of pancreas, elevated the levels of antioxidant enzymes and up regulate the expression of visfatin and PCNA. PCO analysis was good to fitness and prediction distinguishes the therapeutic effects of M. roxburghianus from the alloxan induced diabetic rats. MRME has significant role in protecting animals from alloxan-induced diabetic oxidative stress in pancreas and exhibited promising antihyperglycaemic and antioxidant activities along with significant reversal of disturbed antioxidant status and lipid peroxidative damage. Pancreatic architecture and physiology under diabetic oxidative stress have been significantly modulated by MRME and validated as a drug candidate for antidiabetic treatment. M. roxburghianus treatment restores the antioxidant enzyme system and rejuvenates the islets mass in alloxanized rat by accelerating visfatin and PCNA expression in pancreatic tissue.
authors.affiliation: Department of Zoology, Mizoram Central University, Aizawl, 796004 Mizoram, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Roy
authors.affiliation: Department of Zoology, Mizoram Central University, Aizawl, 796004 Mizoram, India.
authors.forename: L
authors.initials: L
authors.lastname: Chenkual
authors.affiliation: Department of Zoology, Mizoram Central University, Aizawl, 796004 Mizoram, India. Electronic address: gurus64@yahoo.com.
authors.forename: G
authors.initials: G
authors.lastname: Gurusubramanian
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015227:Lipid Peroxidation; D036461:Mallotus Plant / Q000737:chemistry; D008315:Malondialdehyde / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D016207:Cytokines; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D054409:Nicotinamide Phosphoribosyltransferase; C504970:nicotinamide phosphoribosyltransferase, rat
keywords: Antioxidant enzymes; Diabetes; Histopathology; Mallotus roxburghianus; PCNA; Visfatin