title: Identification of a Novel Function of Adipocyte Plasma Membrane-Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue.
abstract: Gestational diabetes mellitus (GDM) is considered as an early stage of type 2 diabetes mellitus. In this study, we compared demographic and clinical data between six GDM subjects and six normal glucose tolerance (NGT; healthy controls) subjects and found that homeostasis model of assessment for insulin resistance index (HOMA-IR) increased in GDM. Many previous studies demonstrated that omental adipose tissue dysfunction could induce insulin resistance. Thus, to investigate the cause of insulin resistance in GDM, we used label-free proteomics to identify differentially expressed proteins in omental adipose tissues from GDM and NGT subjects (data are available via ProteomeXchange with identifier PXD003095). A total of 3528 proteins were identified, including 66 significantly changed proteins. Adipocyte plasma membrane-associated protein (APMAP, a.k.a. C20orf3), one of the differentially expressed proteins, was down-regulated in GDM omental adipose tissues. Furthermore, mature 3T3-L1 adipocytes were used to simulate omental adipocytes. The inhibition of APMAP expression by RNAi impaired insulin signaling and activated NFκB signaling in these adipocytes. Our study revealed that the down-regulation of APMAP in omental adipose tissue may play an important role in insulin resistance in the pathophysiology of GDM.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , 555 Zuchongzhi Road, Shanghai 201203, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Jiajing
authors.initials: J
authors.lastname: Yin
authors.forename: Liping
authors.initials: L
authors.lastname: Gu
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , 555 Zuchongzhi Road, Shanghai 201203, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Su
authors.initials: S
authors.lastname: Chen
authors.forename: Qianfang
authors.initials: Q
authors.lastname: Huang
authors.forename: Huifang
authors.initials: H
authors.lastname: Lu
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , 555 Zuchongzhi Road, Shanghai 201203, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.forename: Yufan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongde
authors.initials: Y
authors.lastname: Peng
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism* / Q000503:physiopathology; D000328:Adult; D000818:Animals; D015153:Blotting, Western; D002853:Chromatography, Liquid; D016640:Diabetes, Gestational / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009852:Omentum / Q000378:metabolism; D011247:Pregnancy; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D016328:NF-kappa B; D020543:Proteome
keywords: gestational diabetes mellitus; inflammation; insulin resistance; label-free proteomic analysis