title: Enhanced Diterpene Tanshinone Accumulation and Bioactivity of Transgenic Salvia miltiorrhiza Hairy Roots by Pathway Engineering.
abstract: Tanshinones are health-promoting diterpenoids found in Salvia miltiorrhiza and have wide applications. Here, SmGGPPS (geranylgeranyl diphosphate synthase) and SmDXSII (1-deoxy-D-xylulose-5-phosphate synthase) were introduced into hairy roots of S. miltiorrhiza. Overexpression of SmGGPPS and SmDXSII in hairy roots produces higher levels of tanshinone than control and single-gene transformed lines; tanshinone production in the double-gene transformed line GDII10 reached 12.93 mg/g dry weight, which is the highest tanshinone content that has been achieved through genetic engineering. Furthermore, transgenic hairy root lines showed higher antioxidant and antitumor activities than control lines. In addition, contents of chlorophylls, carotenoids, indoleacetic acid, and gibberellins were significantly elevated in transgenic Arabidopsis thaliana plants. These results demonstrate a promising method to improve the production of diterpenoids including tanshinone as well as other natural plastid-derived isoprenoids in plants by genetic manipulation of the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway.
authors.affiliation: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University , Hangzhou 310018, People's Republic of China.|Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University , Shanghai 200234, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.affiliation: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University , Shanghai 200234, People's Republic of China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University , Shanghai 200234, People's Republic of China.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Ju
authors.affiliation: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University , Shanghai 200234, People's Republic of China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Li
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, People's Republic of China.
authors.forename: Shengxiong
authors.initials: S
authors.lastname: Huang
authors.affiliation: Experiment Center For Teaching & Learning, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University , Hangzhou 310018, People's Republic of China.
authors.forename: Huizhong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University , Shanghai 200234, People's Republic of China.
authors.forename: Guoyin
authors.initials: G
authors.lastname: Kai
mesh_terms: D045784:Abietanes / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D000040:Abscisic Acid / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D004896:Erythritol / Q000031:analogs & derivatives; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering; D010851:Picrates / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics*; D027561:Salvia miltiorrhiza / Q000737:chemistry* / Q000235:genetics*; D013403:Sugar Phosphates; D014166:Transferases
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D045784:Abietanes; D001713:Biphenyl Compounds; D004224:Diterpenes; D010851:Picrates; D010940:Plant Proteins; D013403:Sugar Phosphates; C021751:tanshinone; D000040:Abscisic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase; D004896:Erythritol
keywords: MEP pathway; Salvia miltiorrhiza; hairy roots; isoprenoids; tanshinone