title: Chronical sleep interruption-induced cognitive decline assessed by a metabolomics method.
abstract: Good sleep is necessary for optimal health, especially for mental health. Insomnia, sleep deprivation will make your ability to learn and memory impaired. Nevertheless, the underlying pathophysiological mechanism of sleep disorders-induced cognitive decline is still largely unknown. In this study, the sleep deprivation of animal model was induced by chronical sleep interruption (CSI), the behavioral tests, biochemical index determinations, and a liquid chromatography-mass spectrometry (LC-MS) based serum metabolic profiling analysis were performed to explore the effects of CSI on cognitive function and the underlying mechanisms. After 14-days CSI, the cognitive function of the mice was evaluated by new objects preference (NOP) task and temporal order judgment (TOJ) task. Serum corticosterone (CORT), and brain Malondialdehyde (MDA), Superoxide Dismutase (SOD), and Catalase (CAT) levels were determined by ELISA kits. Data were analyzed by Principal Component Analysis (PCA), Partial Least Squares project to latent structures-Discriminant Analysis (PLS-DA), and Student's t-test. We found that the cognitive function of the mice was significantly affected by CSI. Besides, levels of CORT and MDA were higher, and SOD and CAT were lower in CSI mice than those of control. Obvious body weight loss of CSI mice was also observed. Thirteen potential serum biomarkers including choline, valine, uric acid, allantoic acid, carnitines, and retinoids were identified. Affected metabolic pathways involve metabolism of purine, retinoid, lipids, and amino acid. These results showed that CSI can damage the cognitive performance notably. The cognitive decline may ascribe to excessive oxidative stress and a series of disturbed metabolic pathways.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Wu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China.
authors.forename: Guang-qing
authors.initials: GQ
authors.lastname: Song
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Space medicine Fundamentation and Application, China Astronaut Research and Training Center, Beijing 100093, China.
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space medicine Fundamentation and Application, China Astronaut Research and Training Center, Beijing 100093, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Space medicine Fundamentation and Application, China Astronaut Research and Training Center, Beijing 100093, China.
authors.forename: Shan-guang
authors.initials: SG
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China; Division of Stem Cell Regulation and Application, Hunan University of Chinese Medicine, Changsha 410208, Hunan, China. Electronic address: liuxinmin@hotmail.com.
authors.forename: Xin-min
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China. Electronic address: qchang@implad.ac.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chang
mesh_terms: D000818:Animals; D001835:Body Weight; D002374:Catalase / Q000097:blood; D002853:Chromatography, Liquid; D003072:Cognition Disorders / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D007600:Judgment / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D021641:Recognition, Psychology / Q000502:physiology; D012893:Sleep Wake Disorders / Q000150:complications*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D003345:Corticosterone
keywords: Chronical sleep interruption; Learning and memory; Mice; New objects preference task; Serum metabolic profiling; Temporal order judgment task