title: Possible regulation of caveolar endocytosis and flattening by phosphorylation of F-BAR domain protein PACSIN2/Syndapin II.
abstract: Caveolae are flask-shaped invaginations of the plasma membrane. The BAR domain proteins form crescent-shaped dimers, and their oligomeric filaments are considered to form spirals at the necks of invaginations, such as clathrin-coated pits and caveolae. PACSIN2/Syndapin II is one of the BAR domain-containing proteins, and is localized at the necks of caveolae. PACSIN2 is thought to function in the scission and stabilization of caveolae, through binding to dynamin-2 and EHD2, respectively. These two functions are considered to be switched by PACSIN2 phosphorylation by protein kinase C (PKC) upon hypotonic stress and sheer stress. The phosphorylation decreases the membrane binding affinity of PACSIN2, leading to its removal from caveolae. The removal of the putative oligomeric spiral of PACSIN2 from caveolar membrane invaginations could lead to the deformation of caveolae. Indeed, PACSIN2 removal from caveolae is accompanied by the recruitment of dynamin-2, suggesting that the removal provides space for the function of dynamin-2. Otherwise, the removal of PACSIN2 decreases the stability of caveolae, which could result in the flattening of caveolae. In contrast, an increase in the amount of EHD2 restored caveolar stability. Therefore, PACSIN2 at caveolae stabilizes caveolae, but its removal by phosphorylation could induce both caveolar endocytosis and flattening.
authors.affiliation: a Institute of Biotechnology; University of Helsinki ; Helsinki , Finland.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Senju
authors.affiliation: b Laboratory of Molecular Medicine and Cell Biology; Graduate School of Biosciences; Nara Institute of Science and Technology ; Ikoma , Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Suetsugu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D021941:Caveolae / Q000378:metabolism*; D004705:Endocytosis; D006801:Humans; D010766:Phosphorylation; D055232:Proline-Rich Protein Domains; D011499:Protein Processing, Post-Translational
chemical_list: D048868:Adaptor Proteins, Signal Transducing
keywords: BAR domain; caveolae; mechanical stress; phosphorylation; protein kinase C