title: Single cell imprinting on the surface of Ag-ZnO bimetallic nanoparticle modified graphene oxide sheets for targeted detection, removal and photothermal killing of E. Coli.
abstract: A very cost-effective, fast, sensitive and specific imprinted polymer modified electrochemical sensor for the targeted detection, removal and destruction of Escherichia coli bacteria was developed onto the surface of Ag-ZnO bimetallic nanoparticle and graphene oxide nanocomposite. The nanocomposite played a dual role in this work, as a platform for imprinting of bacteria as well as a participated in their laser-light induced photo killing. In terms of sensing, our proposed sensor can detect E. Coli as few as 10CFUmL-1and capture 98% of bacterial cells from their very high concentrated solution (105CFUmL-1). Similarly to the quantitative detection, we have also investigated the quantitative destruction of E. Coli and found that 16.0cm2area of polymer modified glass plate is sufficient enough to kill 105CFUmL-1in the small time span of 5 minutes. The obtained results suggest that our proposed sensor have potential to serve as a promising candidate for specific and quantitative detection, removal as well as the destruction of a variety of bacterial pathogens.
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826004, India.
authors.forename: Ekta
authors.initials: E
authors.lastname: Roy
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826004, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Patra
authors.affiliation: Smart Materials and Biodevices, Biosensors and Bioelectronics Centre, IFM-Linköpings Universitet, 581 83 Linköping, Sweden.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826004, India. Electronic address: rshmmadhuri@gmail.com.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Madhuri
authors.affiliation: Functional Nanomaterials Research Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad, Jharkhand 826004, India.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Sharma
mesh_terms: D004926:Escherichia coli / Q000166:cytology / Q000302:isolation & purification* / Q000528:radiation effects; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control; D006108:Graphite / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D007834:Lasers; D054802:Molecular Imprinting / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013242:Sterilization / Q000379:methods*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D011108:Polymers; D012834:Silver; D006108:Graphite; D015034:Zinc Oxide
keywords: Bacteria sensing; GO-Ag@ZnO nanocomposite; Imprinted polymer; Photothermal killing; Removal