title: Hypoxia Enhances Direct Reprogramming of Mouse Fibroblasts to Cardiomyocyte-Like Cells.
abstract: Recent work has shown that mouse and human fibroblasts can be reprogrammed to cardiomyocyte-like cells with a combination of transcription factors. Current research has focused on improving the efficiency and mechanisms for fibroblast reprogramming. Previously, it has been reported that hypoxia enhances fibroblast cell reprogramming to pluripotent stem cells. In this study, we observed that 6 h of hypoxic conditions (2% oxygen) on newborn mouse dermal fibroblasts can improve the efficiency of reprogramming to cardiomyocyte-like cells. Expression of cardiac-related genes and proteins increased at 4 weeks after transfer of three transcription factors (Gata4/Mef2c/Tbx5 [GMT]). However, beating cardiomyocyte cells were not detected. The epigenetic mechanism of hypoxia-induced fibroblast reprogramming to cardiomyocyte cells requires further study.
authors.affiliation: 1 Department of Histology and EmbryologyHarbin Medical University , Harbin, China, 150081 .
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 Department of Histology and EmbryologyHarbin Medical University , Harbin, China, 150081 .
authors.forename: Shujun
authors.initials: S
authors.lastname: Shi
authors.affiliation: 1 Department of Histology and EmbryologyHarbin Medical University , Harbin, China, 150081 .
authors.forename: Huiwen
authors.initials: H
authors.lastname: Liu
authors.affiliation: 2 University Hospital Fertility Center, Case Western Reserve University , Cleveland, OH, USA, 44122 .
authors.forename: Li
authors.initials: L
authors.lastname: Meng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia / Q000502:physiology; D054338:Cell Transdifferentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000235:genetics / Q000502:physiology*; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000502:physiology*; D050991:GATA4 Transcription Factor / Q000235:genetics; D064326:MEF2 Transcription Factors / Q000235:genetics; D051379:Mice; D032383:Myocytes, Cardiac / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D050991:GATA4 Transcription Factor; C484171:Gata4 protein, mouse; D064326:MEF2 Transcription Factors; C578041:Mef2c protein, mouse; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5