title: A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development.
abstract: Mammary gland development is induced by the actions of various hormones to form a structure consisting of collecting ducts and milk-secreting alveoli, which comprise two types of epithelial cells known as luminal and basal cells. These cells adhere to each other by cell adhesion apparatuses whose roles in hormone-dependent mammary gland development remain largely unknown. Here we identified a novel cell adhesion apparatus at the boundary between the luminal and basal cells in addition to desmosomes. This apparatus was formed by the trans-interaction between the cell adhesion molecules nectin-4 and nectin-1, which were expressed in the luminal and basal cells, respectively. Nectin-4 of this apparatus further cis-interacted with the prolactin receptor in the luminal cells to enhance the prolactin-induced prolactin receptor signaling for alveolar development with lactogenic differentiation. Thus, a novel nectin-mediated cell adhesion apparatus regulates the prolactin receptor signaling for mammary gland development.
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and; Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology and; Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Kitayama
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and; Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology and.
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Mizutani
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Maruoka
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and; Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology and.
authors.forename: Kenji
authors.initials: K
authors.lastname: Mandai
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Sakakibara
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ueda
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
authors.forename: Takahide
authors.initials: T
authors.lastname: Komori
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology and.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimono
authors.affiliation: From the Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan and; Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology and. Electronic address: ytakai@med.kobe-u.ac.jp.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism*; D002450:Cell Communication; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008321:Mammary Glands, Animal / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D008810:Mice, Inbred C57BL; D000075983:Nectins; D011388:Prolactin / Q000378:metabolism; D011981:Receptors, Prolactin / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D015815:Cell Adhesion Molecules; C000619010:NECTIN1 protein, human; C000619015:Nectin1 protein, mouse; D000075983:Nectins; D011981:Receptors, Prolactin; C486698:Nectin4 protein, mouse; D011388:Prolactin
keywords: Janus kinase (JAK); cell adhesion; cell-cell interaction; mammary gland; nectin-1; nectin-4; prolactin