title: [Progress in transcriptional studies].
abstract: Gene expression exhibits temporal and spatial patterns to response environmental changes and growth cycle. Gene expression is under strict control at different levels among which control at transcription level is the predominant mode, especially in prokaryotes. In this review, we summarized the new developments of methods used in transcriptional studies, including modifications and improvements of the classic methods, such as gel-shift assay, DNA foot printing, and in vivo reporter system. In addition, we introduced examples to apply new methods, such as surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) to characterize protein-DNA, ligand-protein, and ligand-protein-DNA interactions. The collection of these methods and their application could guide and accelerate relevant studies.
authors.forename: Junyang
authors.initials: J
authors.lastname: Wang
authors.forename: Weishan
authors.initials: W
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.forename: Keqian
authors.initials: K
authors.lastname: Yang
mesh_terms: D002151:Calorimetry; D018983:DNA Footprinting; D015870:Gene Expression; D008024:Ligands; D011506:Proteins; D020349:Surface Plasmon Resonance; D014158:Transcription, Genetic
chemical_list: D008024:Ligands; D011506:Proteins