title: Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.
abstract: We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
authors.forename: Christof
authors.initials: C
authors.lastname: Angermueller
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Clark
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.|Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Heather J
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Iain C
authors.initials: IC
authors.lastname: Macaulay
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Mabel J
authors.initials: MJ
authors.lastname: Teng
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.|Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|Medical Research Council Functional Genomics Unit, University of Oxford, UK.
authors.forename: Tim Xiaoming
authors.initials: TX
authors.lastname: Hu
authors.affiliation: Bioinformatics Group, Babraham Institute, Cambridge, UK.
authors.forename: Felix
authors.initials: F
authors.lastname: Krueger
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Smallwood
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|Medical Research Council Functional Genomics Unit, University of Oxford, UK.
authors.forename: Chris P
authors.initials: CP
authors.lastname: Ponting
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|Department of Human Genetics, Katholieke Universiteit (KU) Leuven, Leuven, Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Voet
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.
authors.forename: Gavin
authors.initials: G
authors.lastname: Kelsey
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
authors.forename: Oliver
authors.initials: O
authors.lastname: Stegle
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.|Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Wolf
authors.initials: W
authors.lastname: Reik
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000502:physiology*; D044127:Epigenesis, Genetic / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D014157:Transcription Factors