title: Selective binding and magnetic separation of histidine-tagged proteins using Fe3O4/Cu-apatite nanoparticles.
abstract: Hierarchical Fe3O4@Cu-apatite nanoparticles (NPs) were synthesized via a facile hydrothermal method. The Fe3O4 cores present spherical shape and have a mean diameter of 300nm, and the Cu-apatite shell with thickness of about 50nm is composed of a large number of sheets. Using the high affinity of Cu(2+) on the surface toward histidine tags, the Fe3O4@Cu-apatite NPs can be applied to enrich and magnetically separate histidine tagged (His-tagged) proteins directly from the mixture of lysed cells. Research results indicated that the Fe3O4@Cu-apatite NPs presented negligible nonspecific protein adsorption and high protein binding ability.
authors.affiliation: Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Li
authors.affiliation: Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, PR China. Electronic address: zhaoyb902@henu.edu.cn.
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D003300:Copper / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D052203:Ferrosoferric Oxide / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D008280:Magnetics; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D011506:Proteins / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D011506:Proteins; D006639:Histidine; D003300:Copper; D052203:Ferrosoferric Oxide
keywords: Fe(3)O(4)@Cu-apatite; His-tagged protein; Preparation; Separation