title: Physiological and molecular analysis on root growth associated with the tolerance to aluminum and drought individual and combined in Tibetan wild and cultivated barley.
abstract: The drought-stimulated gene expression of NCED, SUS, and KS - DHN and ABA signal cross-talk with other phytohormones maintains barley root growth under drought stress at pH 4.0 plus polyethylene glycol plus aluminum. Aluminum (Al) toxicity and drought are two major factors that limit barley production. In this work, the individual and combined effects of Al/acid and polyethylene glycol (PEG 6000) induced drought stress that suppressed root growth and caused oxidative damage as characterized by increased H2O2 and O2(.-) accumulation. The wild-barley genotypes, XZ5 and XZ29, exhibited a higher tolerance than the two cultivars Dayton (Al tolerant) and Tadmor (drought tolerant) under combined stress (pH 4.0 + PEG + Al). The oxidative damage induced by PEG was more severe at pH 4.0 than at pH 6.0. In XZ29, the highest root secretion of malate and citrate was recorded, and the least Al uptake in the four genotypes. In XZ5, a peak accumulation of ABA and minor synthesis of zeatin riboside and ethylene were found being essential in maintaining primary root elongation and root hair development. PEG-induced drought stress repressed Al uptake in root tips, with a lower increase in callose formation and HvMATE (Hordeum vulgare multidrug and toxic compound exudation) expression compared to Al-induced callose production. Stress by pH 4.0 + PEG + Al up-regulated 9-cis-epoxycarotenoid dioxygenase (NCED) which is involved in ABA biosynthesis. Such treatment stimulated the regulation of ABA-dependent genes sucrose synthase (SUS) and KS-type dehydrin (KS-DHN) in root tips. Our results suggest that the tolerance ranking to pH 4.0 + PEG + Al stress in Tibetan wild barley by gene expression is closely correlated to physiological indices. The results show that acclimatisation to pH 4.0 + PEG + Al stress involves specific responses in XZ5 and XZ29. The present study provides insights into the effects of Al/acid and drought combined stress on the abundance of physiological indices in the roots of barley varieties.
authors.affiliation: Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China.|Plant Physiology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, 1701, Bangladesh.
authors.forename: Imrul Mosaddek
authors.initials: IM
authors.lastname: Ahmed
authors.affiliation: Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Umme Aktari
authors.initials: UA
authors.lastname: Nadira
authors.affiliation: Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Fangbin
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China. wufeibo@zju.edu.cn.
authors.forename: Feibo
authors.initials: F
authors.lastname: Wu
mesh_terms: D000040:Abscisic Acid / Q000032:analysis / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000032:analysis / Q000378:metabolism; D001467:Hordeum / Q000187:drug effects / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018527:Hydroponics; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000633:toxicity; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics; D018609:Tibet
chemical_list: D005936:Glucans; D010940:Plant Proteins; D011092:Polyethylene Glycols; D000040:Abscisic Acid; D007541:Isopentenyladenosine; C009699:zeatin riboside; C048306:callose; D006861:Hydrogen Peroxide; D000535:Aluminum; D049308:Dioxygenases; C109043:9-cis-epoxy-carotenoid dioxygenase
keywords: Aluminum; Callose; Citrate and malate secretion; Drought; Genotypic difference; Tibetan wild barley (Hordeum vulgare L. ssp. spontaneum)