title: Spontaneous Coassembly of Biologically Active Nanoparticles via Affinity Binding of Heparin-Binding Proteins to Alginate-Sulfate.
abstract: Controlled delivery of heparin-binding (HB) proteins represents a challenge in regenerative medicine strategies. Here, we describe the features of novel nanoparticles (NPs), spontaneously coassembled due to affinity interactions between HB proteins and the semisynthetic anionic polysaccharide, alginate-sulfate. The NPs efficiently encapsulated and protected the proteins from proteolysis. Injection of a combination of NPs encapsulating multiple therapeutic growth factors promoted effective and long-term tissue repair in animal models of severe ischemia (murine model of hindlimb ischemia and acute myocardial infarction in rats). This simple yet efficient NP fabrication method is amenable for clinical use.
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, ‡Regenerative Medicine and Stem Cell (RMSC) Research Center, and §The Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , Beer-Sheva 8410501, Israel.
authors.forename: Emil
authors.initials: E
authors.lastname: Ruvinov
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, ‡Regenerative Medicine and Stem Cell (RMSC) Research Center, and §The Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , Beer-Sheva 8410501, Israel.
authors.forename: Inbar
authors.initials: I
authors.lastname: Freeman
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, ‡Regenerative Medicine and Stem Cell (RMSC) Research Center, and §The Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , Beer-Sheva 8410501, Israel.
authors.forename: Roei
authors.initials: R
authors.lastname: Fredo
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, ‡Regenerative Medicine and Stem Cell (RMSC) Research Center, and §The Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , Beer-Sheva 8410501, Israel.
authors.forename: Smadar
authors.initials: S
authors.lastname: Cohen
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006493:Heparin / Q000008:administration & dosage* / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry; D006614:Hindlimb / Q000473:pathology; D007511:Ischemia / Q000188:drug therapy; D009203:Myocardial Infarction / Q000188:drug therapy; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011485:Protein Binding; D044968:Regenerative Medicine; D013431:Sulfates / Q000737:chemistry
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D004337:Drug Carriers; D006603:Hexuronic Acids; D013431:Sulfates; D020723:Glucuronic Acid; D006493:Heparin
keywords: Affinity binding; alginate-sulfate; coassembly; heparin-binding proteins; nanoparticles; repair