title: Method for collecting mouse milk without exogenous oxytocin stimulation.
abstract: It has been reported that breast-feeding more than 6 months strongly decreases the risk of allergy, diabetes, obesity, and hypertension in humans. In order to understand the mechanisms responsible for this benefit, it is important to evaluate precisely the composition of maternal milk, especially in response to environmental cues. Mouse models offer a unique opportunity to study the impact of maternal milk composition on the development and health of offspring. Oxytocin injection of the dam is usually used to stimulate milk ejection; however, exogenous oxytocin might have deleterious effects under some experimental conditions by modifying milk content as well as the physiology and behavior of the dam. Taking advantage of the natural stimulation of the mammary gland that occurs after the reunion of a dam that has been separated from her pups, we developed a new procedure to collect mouse milk without the injection of oxytocin. This method is easy to use, low-cost ,and non-invasive. Moreover, it provides a sufficient amount of milk for use in a wide range of biological analyses.
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Muranishi
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Parry
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Averous
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Terrisse
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Maurin
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Chaveroux
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Mesclon
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Carraro
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Bruhat
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Fafournoux
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, Saint Genès, Champanelle, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Jousse
mesh_terms: D000818:Animals; D001940:Breast / Q000378:metabolism / Q000502:physiology; D001942:Breast Feeding; D005260:Female; D006801:Humans; D008321:Mammary Glands, Animal / Q000502:physiology*; D051379:Mice; D008892:Milk; D008893:Milk Ejection / Q000502:physiology*; D010121:Oxytocin / Q000494:pharmacology
chemical_list: D010121:Oxytocin
keywords: methodology; milking; mouse; nursing; oxytocin