title: Strain variation in microalgal lipid production during mixotrophic growth with glycerol.
abstract: Algal cultivation at high latitudes is challenged by the relatively low annual solar flux. One possible scenario to overcome this limitation is the use of mixotrophic growth to potentially boost biomass and lipid production. Here the effect of glycerol addition on the growth and lipid production by twelve indigenous microalgae was examined. The results show that there is considerable strain dependent variation in the maximum growth rate under mixotrophic conditions with the addition of glycerol causing in some cases up to a 2.4-fold increase in growth rate and a up to a 1.9-fold increase in biomass. In addition, glycerol increased total lipid production 40-60% in some strains. These results also show the value in screening culture collections for desired traits independent of strain identification since here one (PCH02) of the five Chlorella strains showed a large increase in lipid with glycerol.
authors.affiliation: Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, CP6128 Succursale Centre-ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Kiran
authors.initials: K
authors.lastname: Paranjape
authors.affiliation: Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, CP6128 Succursale Centre-ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Gustavo B
authors.initials: GB
authors.lastname: Leite
authors.affiliation: Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, CP6128 Succursale Centre-ville, Montréal, Québec H3C 3J7, Canada; Life Sciences Research Center, Department of Biology, United States Air Force Academy, 2355 Faculty Drive, USAF Academy, CO 80840, United States. Electronic address: patrick.hallenbeck@umontreal.ca.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Hallenbeck
mesh_terms: D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D013045:Species Specificity
chemical_list: D056804:Biofuels; D008055:Lipids; D005990:Glycerol
keywords: Biofuels; Lipid production; Microalgae; Mixotrophic growth