title: Characterization of isomeric VX nerve agent adducts on albumin in human plasma using liquid chromatography-tandem mass spectrometry.
abstract: This study includes the characterization of isomeric VX organophosphorus adducts on albumin in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). VX or its structural isomers were spiked into a vial containing plasma in order to obtain phosphorylated albumin. After pronase and trypsin digestion, the resulting solutions were analyzed to confirm adduct formation with the amino acid tyrosine on the albumin in human plasma. The LC-MS/MS experiments show that VX and its isomers can be attached to tyrosine on the albumin in human blood. Mass spectrometric studies revealed some interesting fragmentation pathways during the ionization process, such as ethylene, formic acid and ammonia elimination and an intermolecular electrophilic aromatic substitution reaction. The proposed mechanisms for the formation of the fragments were confirmed through the analysis of fragments of deuterated adducts.
authors.affiliation: Department of Science, Payame Noor University (PNU), P.O Box: 19395-4697, Tehran, Iran. Saeidian1980@gmail.com.
authors.forename: Hamid
authors.initials: H
authors.lastname: Saeidian
authors.affiliation: Department of Chemistry, University of Isfahan, P.O Box: 81746-73441, Isfahan, Iran. Mirkhani@sci.ui.ac.ir.
authors.forename: Valioallah
authors.initials: V
authors.lastname: Mirkhani
authors.affiliation: Department of Chemistry, University of Isfahan, P.O Box: 81746-73441, Isfahan, Iran. Defense Chemical Research Lab (DCRL), P.O. Box: 31585-1461, Karaj, Iran.. sadjadmousavie@gmail.com.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Mousavi Faraz
authors.affiliation: Defense Chemical Research Lab (DCRL), P.O. Box: 31585-1461, Karaj, Iran. Naseri@dcrl.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Taghi Naseri
authors.affiliation: Defense Chemical Research Lab (DCRL), P.O. Box: 31585-1461, Karaj, Iran. Babri@dcrl.ir.
authors.forename: Mehran
authors.initials: M
authors.lastname: Babri