title: Self-Catalyzed Assembly of Peptide Scaffolded Nanozyme as a Dynamic Biosensing System.
abstract: In this work, a new strategy of biosensor design is developed based on the assembly of amyloid beta and its multiple interactions with other bioactive species. These interactions can enable amyloid beta peptide as a multifunctional sensing element, so the immobilization of sensing probe and the step-by-step modification of the sensing interface have all been dispensed with. Instead, the kinetics of the assembly of a peptide-based catalytic network serves to convert the quantity of analyte into amplified signal readout. The designed dynamic assembling and biosensing system has also been successfully applied in detecting the activity of polyglutamylation, an essential post translation modification controlling cell skeleton and cell cycle, in biological complex samples. Further studies reveal that the serum abundance of a polyglutamylase, tubulin tyrosine ligase-like protein 12, may show parallel with the degree of development of prostate cancer and the discrimination between early cancerous development and benign conditions. And the obtained result is more distinct than that based on PSA detection, the current gold standard. This study may also point to the prospective of extending this design strategy to broader range of biosensing applications in the future.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University , Nanjing 210093, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University , Nanjing 210093, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing 210008, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University , Nanjing 210093, China.|Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University , Shanghai 200444, China.
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
authors.affiliation: Laboratoire de la Barrière Hémato-encéphalique, Faculté des Sciences, Université d'Artois , rue Souvraz SP18, 62307 Lens Cedex, France.
authors.forename: Yannis
authors.initials: Y
authors.lastname: Karamanos
mesh_terms: D016229:Amyloid beta-Peptides; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D045744:Cell Line, Tumor; D057809:HEK293 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010453:Peptide Synthases / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000378:metabolism; D015203:Reproducibility of Results; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D010455:Peptides; D017430:Prostate-Specific Antigen; D010453:Peptide Synthases; C553200:TTLL12 protein, human; C023213:tyrosyltubulin ligase
keywords: amyloid-beta peptide; catalytically self-propagating assembly; nanozyme; peptide self-assembly; peptide-based biosensing