title: β1,6-branched complex-type N-glycans affect FAK signaling in metastatic melanoma cells.
abstract: Integrin-dependent binding of the cell to extracellular matrix (ECM) is a key activator of the focal adhesion kinase (FAK) signaling pathway. N-glycosylation of integrins affects their interactions with ECM proteins. Using WM266-4 cells with overexpression of β1,6-acetylglucosaminyltransferase V, we showed that β1,6-branched N-glycans increased tyrosine phosphorylation of FAK in metastatic melanoma cells, resulting in enhanced migration on vitronectin (VN). The co-localization of αvβ3 integrin and FAK in focal adhesions of melanoma cells growing on VN indicates their interaction in signal transduction. Melanoma cell migration on VN was mediated by αvβ3 caring overexpressed β1,6-branched structures, important for FAK upregulation.
authors.affiliation: a Department of Glycoconjugate Biochemistry, Institute of Zoology , Jagiellonian University Krakow , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pocheć
authors.affiliation: a Department of Glycoconjugate Biochemistry, Institute of Zoology , Jagiellonian University Krakow , Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Ząbczyńska
authors.affiliation: a Department of Glycoconjugate Biochemistry, Institute of Zoology , Jagiellonian University Krakow , Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Bubka
authors.affiliation: b Department of Evolutionary Immunology, Institute of Zoology , Jagiellonian University Krakow , Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Homa
authors.affiliation: a Department of Glycoconjugate Biochemistry, Institute of Zoology , Jagiellonian University Krakow , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lityńska
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D011134:Polysaccharides / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D039302:Integrin alphaVbeta3; D011134:Polysaccharides; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: 6-branched N-glycans; Focal adhesion kinase; Melanoma; Vitronectin; αvβ3 integrin; β1