title: A highly sensitive biosensor for tumor maker alpha fetoprotein based on poly(ethylene glycol) doped conducting polymer PEDOT.
abstract: Biocompatible polymers, such as poly(ethylene glycol) (PEG), are of great significance in the development of bio-interfaces and biosensors, as they possess excellent biocompatibility and are easy for modification. A novel highly biocompatible polymer composite was synthesized herein through electrochemical polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a PEG derivative, 4-arm PEG terminated with thiol groups. The electrodeposited conducting polymer composite of PEG doped PEDOT (PEDOT/PEG) exhibited flake-like nanostructure, large surface area and outstanding stability. In order to further immobilize antibodies, gold nanoparticles (AuNPs) were introduced to the PEDOT/PEG composite surface through their unique interaction with the thiol groups. AuNPs modified PEDOT/PEG provided a desirable support for the immobilization of various biomolecules such as antibodies for alpha fetoprotein (AFP), a vital tumor biomarker for liver cancer. The fabricated AFP biosensor demonstrated favorable selectivity, high sensitivity, and ultralow detection limit. Furthermore, owing to the presence of PEG polymers that are highly hydrophilic, such AuNPs/PEDOT/PEG based AFP biosensor also exhibited good anti-fouling ability, and it was capable of assaying target AFP in 10% (V/V) human serum samples, indicating highly feasible potential for clinical diagnosis.
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China; College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Cui
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China; Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Song
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China; College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Breast Surgery, The Eighth People's Hospital of Qingdao, Qingdao 266100, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Guo
authors.affiliation: Department of Breast Surgery, The Eighth People's Hospital of Qingdao, Qingdao 266100, PR China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Fan
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China; Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China. Electronic address: 1325536857@qq.com.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Luo
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000379:methods; D004595:Electroplating; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; D000509:alpha-Fetoproteins; C121383:poly(3,4-ethylene dioxythiophene); D011092:Polyethylene Glycols; D006046:Gold
keywords: Alpha fetoprotein; Biosensor; Conducting polymer; Poly(3,4-ethylenedioxythiophene); Poly(ethylene glycol)