title: Sensing parasites: Proteomic and advanced bio-detection alternatives.
abstract: Parasitic diseases have a great impact in human and animal health. The gold standard for the diagnosis of the majority of parasitic infections is still conventional microscopy, which presents important limitations in terms of sensitivity and specificity and commonly requires highly trained technicians. More accurate molecular-based diagnostic tools are needed for the implementation of early detection, effective treatments and massive screenings with high-throughput capacities. In this respect, sensitive and affordable devices could greatly impact on sustainable control programmes which exist against parasitic diseases, especially in low income settings. Proteomics and nanotechnology approaches are valuable tools for sensing pathogens and host alteration signatures within microfluidic detection platforms. These new devices might provide novel solutions to fight parasitic diseases. Newly described specific parasite derived products with immune-modulatory properties have been postulated as the best candidates for the early and accurate detection of parasitic infections as well as for the blockage of parasite development. This review provides the most recent methodological and technological advances with great potential for bio-sensing parasites in their hosts, showing the newest opportunities offered by modern "-omics" and platforms for parasite detection and control.
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA-CSIC), 37008 Salamanca, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sánchez-Ovejero
authors.affiliation: Analytical Chemistry Department, Universidad del País Vasco UPV/EHU, 01006 Vitoria-Gasteiz, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Benito-Lopez
authors.affiliation: Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain; Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, - 00161 Rome, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Casulli
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA-CSIC), 37008 Salamanca, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Siles-Lucas
authors.affiliation: Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain; Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain. Electronic address: mfuentes@usal.es.
authors.forename: Manuel
authors.initials: M
authors.lastname: Fuentes
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA-CSIC), 37008 Salamanca, Spain. Electronic address: raul.manzano@irnasa.csic.es.
authors.forename: Raúl
authors.initials: R
authors.lastname: Manzano-Román
mesh_terms: D000818:Animals; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D010272:Parasitic Diseases / Q000175:diagnosis* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
keywords: Detection platforms; Diagnosis; Microfluidics; Parasitic diseases; Proteomics; Sensors