title: Synthesis, characterization, and application of polysodium N-alkylenyl α-d-glucopyranoside surfactants for micellar electrokinetic chromatography-tandem mass spectrometry.
abstract: Sugar-based ionic surfactants forming micelles are known to suppress ESI of various compounds due to decrease in surface tension upon micelle formation . For the first time, poly (sodium N-undecylenyl-α-d-glucopyranoside 4,6-hydrogen phosphate, (poly-α-d-SUGP) based surfactants with different chain lengths and head groups have been successfully synthesized, characterized, and applied as compatible chiral selector for MEKC-ESI-MS/MS. First, the effect of polymerization concentration of the monomer, α-d-SUGP, was evaluated by enantioseparation of one anionic compound (1,1'-binaphthyl-2,2'diyl-hydrogen phosphate) and one zwitterionic compound (dansylated phenylalanine) in MEKC-UV to find the optimum molar surfactant concentration for polymerization. Next, MEKC-UV and MEKC-MS were compared for the enantioseparation of 1,1'-binaphthyl-2,2'diyl-hydrogen phosphate. The influence of polymeric glucopyranoside based surfactant head groups and carbon chain lengths on chiral Rs was evaluated for two classes of cationic drugs (ephedrine alkaloids and β-blockers). Finally, enantioselective MEKC-MS of ephedrine alkaloids and β-blockers were profiled at their optimum pH 5.0 and 7.0, respectively, using 20 mM NH4 OAc, 25 mM poly-α-d-SUGP at 30 kV and 25°C under optimum spray chamber conditions. The LOD for most of the enantiomers ranges from 10 to 100 ng/mL with S/N of at least ≥3.0.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA, US.
authors.forename: Yijin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA, US.
authors.forename: Baolin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA, US.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA, US.
authors.forename: Shahab A
authors.initials: SA
authors.lastname: Shamsi
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005936:Glucans / Q000737:chemistry*; D008958:Models, Molecular; D013501:Surface-Active Agents / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005936:Glucans; D013501:Surface-Active Agents
keywords: Beta-blockers; Enantioseparations; Ephedrine alkaloids; MEKC-ESI-MS/MS; Poly(sodium N-alkenyl-α-d-glucopyranoside) surfactants