title: Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration.
abstract: In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and the utilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody (ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of pork and beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating methods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbent assay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On the other hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weight of smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are monoclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determine animal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can be developed with an antibody specific to each species smTnI.
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, Florida State University, Florida 32306-1493, USA.
authors.forename: Bong-Sup
authors.initials: BS
authors.lastname: Park
authors.affiliation: School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Young-Kyoung
authors.initials: YK
authors.lastname: Oh
authors.affiliation: School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Min-Jin
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Food Analysis Research Team, Industry Service Research Center, World Institute of Kimchi, Gwangju 500-360, Korea.
authors.forename: Won-Bo
authors.initials: WB
authors.lastname: Shim
keywords: anti-smTnI antibody; fat species identification; fat tissue; meat adulteration; skeletal muscle troponin I