title: Human herpesvirus 6 U11 protein is critical for virus infection.
abstract: All herpesviruses contain a tegument layer comprising a protein matrix; these proteins play key roles during viral assembly and egress. Here, liquid chromatography and tandem mass spectrometry analysis (LC-MS/MS) of proteins from human herpesvirus 6 (HHV-6)-infected cells revealed a possible association between two major tegument proteins, U14 and U11. This association was verified by immunoprecipitation experiments. Moreover, U11 protein was expressed during the late phase of infection and incorporated into virions. Finally, in contrast to its revertant, a U11 deletion mutant could not be reconstituted. Taken together, these results suggest that HHV-6 U11 is an essential gene for virus growth and propagation.
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors.forename: Nora F
authors.initials: NF
authors.lastname: Mahmoud
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawabata
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Department of Immunology, Nanjing Medical University, Nanjing, China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Tang
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Aika
authors.initials: A
authors.lastname: Wakata
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Bochao
authors.initials: B
authors.lastname: Wang
authors.affiliation: Laboratory of Immune Signal, National Institutes of Biomedical Innovation, Health and Nutrition, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Serada
authors.affiliation: Laboratory of Immune Signal, National Institutes of Biomedical Innovation, Health and Nutrition, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Naka
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan. Electronic address: ymori@med.kobe-u.ac.jp.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
mesh_terms: D015654:Herpesvirus 6, Human / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D019349:Roseolovirus Infections / Q000821:virology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015678:Viral Structural Proteins
keywords: HHV-6; Tegument protein; U11; U14