title: Solid-State NMR of PEGylated Proteins.
abstract: PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic-level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid-state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use.
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Enrico
authors.identifier: http://orcid.org/0000-0001-7708-9208
authors.initials: E
authors.lastname: Ravera
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Silvia
authors.identifier: http://orcid.org/0000-0002-1924-5046
authors.initials: S
authors.lastname: Ciambellotti
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Linda
authors.identifier: http://orcid.org/0000-0002-0795-9594
authors.initials: L
authors.lastname: Cerofolini
authors.affiliation: Giotto Biotech S.r.l. Via Madonna del Piano 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Tommaso
authors.identifier: http://orcid.org/0000-0003-2394-818X
authors.initials: T
authors.lastname: Martelli
authors.affiliation: Giotto Biotech S.r.l. Via Madonna del Piano 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kozyreva
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Caterina
authors.identifier: http://orcid.org/0000-0002-1023-0067
authors.initials: C
authors.lastname: Bernacchioni
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Stefano
authors.identifier: http://orcid.org/0000-0002-2292-8163
authors.initials: S
authors.lastname: Giuntini
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Marco
authors.identifier: http://orcid.org/0000-0002-8440-1690
authors.initials: M
authors.lastname: Fragai
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Paola
authors.identifier: http://orcid.org/0000-0002-7683-8614
authors.initials: P
authors.lastname: Turano
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy. claudioluchinat@cerm.unifi.it.|Giotto Biotech S.r.l. Via Madonna del Piano 6, 50019, Sesto Fiorentino, FI, Italy. claudioluchinat@cerm.unifi.it.
authors.forename: Claudio
authors.identifier: http://orcid.org/0000-0003-2271-8921
authors.initials: C
authors.lastname: Luchinat
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D011092:Polyethylene Glycols
keywords: PEGylated proteins; biopharmaceuticals; protein modification; protein structures; solid-state NMR