title: Ferric nitrilotriacetate (Fe-NTA)-induced reactive oxidative species protects human hepatic stellate cells from apoptosis by regulating Bcl-2 family proteins and mitochondrial membrane potential.
abstract: Reactive oxidative species (ROS)-induced apoptosis of human hepatic stellate (HSC) is one of the treatments for liver fibrosis. However, how ROS (reactive oxygen species) affect HSC apoptosis and liver fibrosis is still unknown. In our study, ROS in human HSC cell line LX-2 was induced by ferric nitrilotriacetate (Fe-NTA) and assessed by superoxide dismutase (SOD) activity and methane dicarboxylic aldehyde (MDA) level. We found that in LX2 cells Fe-NTA induced notable ROS, which played a protective role in HSCs cells apoptosis by inhibiting Caspase-3 activation. Fe-NTA-induced ROS increased mRNA and protein level of anti-apoptosis Bcl-2 and decreased mRNA protein level of pro-apoptosis gene Bax, As a result, maintaining mitochondrial membrane potential of HSCs. Fe-NTA-induced ROS play a protective role in human HSCs by regulating Bcl-2 family proteins and mitochondrial membrane potential.
authors.affiliation: Medical College, Qingdao UniversityQingdao, China; Department of Gastroenterology, Jining First People's HospitalJining, Shandong Province, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Rheumatology, Jining First People's Hospital Jining, Shandong Province, China.
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital Qingdao, China.
authors.forename: Yong-Ning
authors.initials: YN
authors.lastname: Xin
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital Jining, Shandong Province, China.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Ji
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital Jining, Shandong Province, China.
authors.forename: Rui-Jin
authors.initials: RJ
authors.lastname: Xie
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital Qingdao, China.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Xuan
keywords: Bax mitochondrial membrane potential; Bcl-2; Liver fibrosis; apoptosis; ferric nitrilotriacetate; reactive oxidative species