title: Preparative separation of sesamin and sesamolin from defatted sesame meal via centrifugal partition chromatography with consecutive sample injection.
abstract: A preparative separation method using consecutive sample injection centrifugal partition chromatography (CPC) was developed to obtain sesamin and sesamolin from defatted sesame meal extracts. A two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (8:2:8:2, v/v) was applied in reversed-phase mode (descending mode). Preliminary experiments with an SCPC-100 (column volume: 100mL) were performed to select the appropriate two-phase solvent system and sample injection times; these parameters were then used with an SCPC-1000 (column volume: 1000mL) in a 10-fold scale-up preparative run. A sample containing 3g of crude extract was consecutively injected four times onto the SCPC-1000, which yielded 328mg of sesamin and 168mg of sesamolin. These compounds were analyzed by high-performance liquid chromatography and determined to have purities of 95.6% and 93.9%, respectively. Sesamin and sesamolin (30μM) increased antioxidant response element (ARE) luciferase activity 2.6-fold and 1.9-fold, respectively.
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea.
authors.forename: Je-Seung
authors.initials: JS
authors.lastname: Jeon
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea.
authors.forename: Chae Lee
authors.initials: CL
authors.lastname: Park
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea.
authors.forename: Ahmed Shah
authors.initials: AS
authors.lastname: Syed
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea.
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Daegu Haany University, 1, Hannydaero, Gyeongsan, Gyeongsangbukdo 38610, Republic of Korea.
authors.forename: Il Je
authors.initials: IJ
authors.lastname: Cho
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea. Electronic address: chulykim@hanyang.ac.kr.
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
mesh_terms: D002498:Centrifugation / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004149:Dioxoles / Q000302:isolation & purification*; D056945:Hep G2 Cells; D006801:Humans; D017705:Lignans / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D031685:Sesamum / Q000737:chemistry
chemical_list: D004149:Dioxoles; D017705:Lignans; D010936:Plant Extracts; C054124:sesamolin; C054125:sesamin
keywords: Antioxidant response element; Centrifugal partition chromatography; Consecutive sample injection; Sesamin; Sesamolin