title: Kiyoteru Tokuyasu: a pioneer of cryo-ultramicrotomy.
abstract: In July 2015 Professor K.T. Tokuyasu passed away in San Diego giving us the opportunity to reflect on the contribution this electron microscopist made to the field of immunocytochemistry. His work provided a sensitive, minimally invasive approach to producing thin sections of biological material for labeling with antibodies. His approach has been applied to a wide range of biological applications and provided important information on cellular processes.
authors.affiliation: Department of Biosciences, University of Oslo, Oslo, Norway.
authors.forename: Gareth
authors.initials: G
authors.lastname: Griffiths
authors.affiliation: Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: Slot
authors.affiliation: Oak Crest Institute of Science, Monrovia, California, U.S.A.
authors.forename: Paul
authors.initials: P
authors.lastname: Webster
mesh_terms: D005615:Freezing; D049673:History, 20th Century; D049674:History, 21st Century; D008867:Microtomy / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*
keywords: CEMOVIS; Cryoultramicrotomy; Tokuyasu method; colloidal gold; cryo-EM; cryo-protection; ferritin; immunocytochemistry; methyl cellulose; sectioning; thawed cryosections; vitrification