title: Osteogenic differentiation and gene expression of dental pulp stem cells under low-level laser irradiation: a good promise for tissue engineering.
abstract: The effects of low-level laser therapy (LLLT) has been the focus of recent studies as being assumed responsible for promoting photostimulatory and photobiomodulatory effects in vivo and in vitro, increasing cell metabolism, improving cell regeneration and invoking an anti-inflammatory response. A positive effect of LLLT on the bone proliferation of some cell types has been observed, but little is known about its effect on dental pulp stem cells (DPSCs). Here, we accurately describe the technical procedure to isolate mesenchymal DPSCs, and assay their osteogenic capacity when irradiated with an LLLT source. These preliminary results show that LLLT irradiation influences the in vitro proliferation of DPSCs and increases the expression of essential proteins for bone formation, although it is necessary to carry out further experiments on other cell types and to uniform the methodological designs.
authors.affiliation: Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ballini
authors.affiliation: Unit of Dentistry, IRCCS San Raffaele Scientific Institute, Vita e Salute San Raffaele University, Milano, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Mastrangelo
authors.affiliation: Unit of Dentistry, IRCCS San Raffaele Scientific Institute, Vita e Salute San Raffaele University, Milano, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gastaldi
authors.affiliation: Department of Periodontology and Oral Implantology, Rural Dental College Loni Maharashtra, India.
authors.forename: L
authors.initials: L
authors.lastname: Tettamanti
authors.affiliation: University Hospital Bari-Policlinics, Medical Genetics Unit Bari, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Bukvic
authors.affiliation: Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro Bari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Cantore
authors.affiliation: Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro Bari, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Cocco
authors.affiliation: Department of Oral Science, Insubria University of Varese, Varese, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Saini
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari Aldo Moro Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Desiate
authors.affiliation: Unit of Dentistry, IRCCS San Raffaele Scientific Institute, Vita e Salute San Raffaele University, Milano, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Gherlone
authors.affiliation: Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro Bari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Scacco
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology* / Q000528:radiation effects; D006801:Humans; D028022:Low-Level Light Therapy; D010012:Osteogenesis / Q000528:radiation effects*; D000076182:Sp7 Transcription Factor; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D023822:Tissue Engineering / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors