title: Characterization of swine testicular cell line as immature porcine Sertoli cell line.
abstract: Swine testicular (ST) cell line is isolated from swine fetal testes and has been widely used in biomedical research fields related to pig virus infection. However, the potential benefit and utilization of ST cells in boar reproductive studies has not been fully explored. As swine fetal testes mainly contain multiple types of cells such as Leydig cells, Sertoli cells, gonocytes, and peritubular myoid cells, it is necessary to clarify the cell type of ST cell line. In this study, we identified ST cell line was a collection of Sertoli cells by analyzing the unique morphological characteristic with satellite karyosomes and determining the protein expression of two markers (androgen-binding protein, ABP; Fas ligand, FASL) of Sertoli cells. Then ST cells were further confirmed to be immature Sertoli cells by examining the expression of three markers (anti-Mullerian hormone, AMH; keratin 18, KRT18; follicle-stimulating hormone receptor, FSHR). In conclusion, ST cells are a collection of immature Sertoli cells which can be good experimental materials for the researches involved in Sertoli cell functions and maturation, or even in boar reproductions.
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Changping
authors.initials: C
authors.lastname: Ma
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Huibin
authors.initials: H
authors.lastname: Song
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Guan
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Informatics, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Xuanyan
authors.initials: X
authors.lastname: Xia
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China. lifener@mail.hzau.edu.cn.
authors.forename: Fenge
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D048430:Cell Shape; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D008297:Male; D008954:Models, Biological; D012708:Sertoli Cells / Q000166:cytology* / Q000648:ultrastructure; D013194:Staining and Labeling; D013552:Swine; D013737:Testis / Q000166:cytology*
chemical_list: D015415:Biomarkers
keywords: Boar reproduction; Satellite karyosomes; Sertoli cell; Swine testicular cell