title: Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM(+) B cells.
abstract: Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM(+) B cells, because after four days of stimulation the IgM(+) fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM(+) cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B.
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan; Laboratory of Fish Immunology, Institute of Infectology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Miyata
authors.affiliation: Laboratory of Comparative Immunology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Katakura
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Bioresourse and Bioenvironment, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagasawa
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Shibasaki
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yabu
authors.affiliation: Laboratory of Fish Immunology, Institute of Infectology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Fischer
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Minami-ise, Mie 516-0193, Japan.
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
authors.affiliation: Laboratory of Comparative Immunology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: moritomo.tadaaki@nihon-u.ac.jp.
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Moritomo
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002347:Carps / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D029941:Fish Proteins / Q000235:genetics* / Q000276:immunology; D007075:Immunoglobulin M / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000276:immunology; D007668:Kidney / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013154:Spleen / Q000276:immunology
chemical_list: D029941:Fish Proteins; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D015847:Interleukin-4
keywords: B cell; Carp; Fish; IL-4; IL-4/13B; Proliferation; Recombinat protein