title: Toll-like Receptor Expression Profile of Human Dental Pulp Stem/Progenitor Cells.
abstract: BACKGROUND
  Human dental pulp stem/progenitor cells (DPSCs) show remarkable regenerative potential in vivo. During regeneration, DPSCs may interact with their inflammatory environment via toll-like receptors (TLRs). The present study aimed to depict for the first time the TLR expression profile of DPSCs.
  
  
  METHODS
  Cells were isolated from human dental pulp, STRO-1-immunomagnetically sorted, and seeded out to obtain single colony-forming units. DPSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, and CD146 expression and for their multilineage differentiation potential. After incubation of DPSCs in basic or inflammatory medium (interleukin-1β, interferon-γ, interferon-α, tumor necrosis factor-α), TLR expression profiles were generated (DPSCs and DPSCs-i).
  
  
  RESULTS
  DPSCs showed all characteristics of stem/progenitor cells. In basic medium DPSCs expressed TLRs 1-10 in different quantities. The inflammatory medium upregulated the expression of TLRs 2, 3, 4, 5, and 8, downregulated TLRs 1, 7, 9, and 10, and abolished TLR6.
  
  
  CONCLUSIONS
  The current study describes for the first time the distinctive TLR expression profile of DPSCs in uninflamed and inflamed conditions.
authors.affiliation: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany; Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt. Electronic address: karim.fawzy@gmail.com.
authors.forename: Karim M
authors.initials: KM
authors.lastname: Fawzy El-Sayed
authors.affiliation: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
authors.forename: Pauline
authors.initials: P
authors.lastname: Klingebiel
authors.affiliation: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
authors.forename: Christof E
authors.initials: CE
authors.lastname: Dörfer
mesh_terms: D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000096:biosynthesis* / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D051193:Toll-Like Receptors
keywords: Flow cytometry; TLR; polymerase chain reaction; pulp; stem cells