title: LincRNAs and base modifications of p53 induced by arsenic methylation in workers.
abstract: Arsenic (As) metabolites could induce methylation changes of DNA and base modifications of p53, which play role in the toxicity of As. LincRNAs should play a key regulatory role in the p53 transcriptional response. There were 43 workers producing As trioxide, 36 workers who stopped exposure to As trioxide about 85 days ago, and 24 individuals as control group. Three As species in urine were measured, and primary and secondary methylation indexes, iAs%, MMA% and DMA% were calculated. RT-PCR was performed to detect the expression of 7 LincRNAs and the base modifications of exon 5, 6, 7, and 8 of p53. The concentrations of urinary As were high in workers. Compared to control group, significant changes for 5 LincRNAs in workers producing As trioxide were found (P < 0.05), and there were significant base modifications of p53 in workers came from the two plants (P < 0.05). There exist various correlations between different exon base modifications of p53 and expressions of LincRNAs (P < 0.05). The closely positive correlations between MMA/DMA and MEG3/TUG1/HOTAIR/MALAT1 were found, but negative correlation between DMA/MALAT1 and the base modifications of exon 7 and 8 of p53 were found also (P < 0.05). LincRNAs and base modifications of p53 could be induced by As, MALAT1 and the base modifications of exon 7 and 8 of p53 could play unique roles in epigenic changes. These findings suggest potentially widespread roles of p53 and relative RNAs in arsenic workers, which may be caused by As metabolism.
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No.158, Dongsi Street, Kunming, Yunnan, 650022, China; Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: dongsijiehua@sina.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No.158, Dongsi Street, Kunming, Yunnan, 650022, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.affiliation: Public Health College, Kunming Medical University, No. 295 Xichang Road, Kunming, Yunnan, 650032, China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No.158, Dongsi Street, Kunming, Yunnan, 650022, China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No.158, Dongsi Street, Kunming, Yunnan, 650022, China.
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No.158, Dongsi Street, Kunming, Yunnan, 650022, China.
authors.forename: Shuqiao
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Occupational Health, Honghe Autonomous Prefecture of Ha'ni and Yi Ethnic Minority Center for Disease Control and Prevention, Guanlan Road, Mengzi City, Yunnan, 661100, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No.158, Dongsi Street, Kunming, Yunnan, 650022, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: wut@mails.tjmu.edu.cn.
authors.forename: Tangchun
authors.initials: T
authors.lastname: Wu
mesh_terms: D000328:Adult; D001151:Arsenic / Q000737:chemistry* / Q000633:toxicity*; D019175:DNA Methylation / Q000187:drug effects*; D044127:Epigenesis, Genetic / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects*; D062085:RNA, Long Noncoding / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; D016159:Tumor Suppressor Protein p53; D001151:Arsenic
keywords: As; Linc RNAs; Noncoding RNAs; p53