title: The relationship between aquaglyceroporin expression and development of fatty liver in diet-induced obesity and ob/ob mice.
abstract: Aquaporin (AQP) 7 and AQP9 are subcategorised as aquaglyceroporins which transport glycerin in addition to water. These AQPs may play a role in the homeostasis of energy metabolism. We examined the effect of AQP7, AQP9, and lipid metabolism-related gene expression in obese mice. In diet-induced obese (DIO) mice, excess lipid accumulated in the liver, which was hyperleptinemic and hyperinsulinemic. Hepatic AQP9 gene expression was significantly increased in both DIO and ob/ob mice compared to controls. The mRNA expression levels of fatty acid and triglyceride synthesis-related genes and fatty acid β oxidation-related genes in the liver were also higher in both mouse models, suggesting that triglyceride synthesis in this organ is promoted as a result of glycerol release from adipocytes. Adipose AQP7 and AQP9 gene expressions were increased in DIO mice, but there was no difference in ob/ob mice compared to wild-type mice. In summary, adipose AQP7 and AQP9 gene expressions are increased by diet-induced obesity, indicating that this is one of the mechanisms by which lipid accumulates in response to a high fat diet, not the genetic mutation of ob/ob mice. Hepatic AQP9 gene expression was increased in both obesity model mice. AQP7 and AQP9 therefore have the potential of defining molecules for the characterisation of obesity or fatty liver and may be a target molecules for the treatment of those disease.
authors.affiliation: Department of Health and Nutrition, University of Human Arts and Sciences, Saitama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirako
authors.affiliation: Department of Anatomy, Showa University School of Medicine, Tokyo, Japan; Wakayama Clinic, Machida-shi, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wakayama
authors.affiliation: Department of Clinical Dietetics & Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
authors.forename: Hyounju
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Clinical Dietetics & Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Iizuka
authors.affiliation: Department of Clinical Dietetics & Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
authors.forename: Akiyo
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Department of Internal Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Wada
authors.affiliation: Hoshi University School of Pharmacy and Pharmaceutical Sciences Global Research Center for Innovative Life Science Peptide Drug Innovation, Tokyo, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Kimura
authors.affiliation: Tokyo Shokuryo Dietitian Academy, Tokyo, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Okabe
authors.affiliation: Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Sakagami
authors.affiliation: Department of Biochemistry, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Mamiko
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Department of Exercise and Sports Physiology, Hoshi University School of Pharmacy and Pharmaceutical Science, Tokyo, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Takenoya
authors.affiliation: Hoshi University School of Pharmacy and Pharmaceutical Sciences Global Research Center for Innovative Life Science Peptide Drug Innovation, Tokyo, Japan. Electronic address: shioda@hoshi.ac.jp.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D051397:Aquaglyceroporins / Q000378:metabolism; D020346:Aquaporins / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism*; D015870:Gene Expression; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008820:Mice, Obese; D009765:Obesity / Q000150:complications / Q000209:etiology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: C491927:Aqp7 protein, mouse; C491638:Aqp9 protein, mouse; D051397:Aquaglyceroporins; D020346:Aquaporins; D005227:Fatty Acids; D012333:RNA, Messenger; D014280:Triglycerides; D005990:Glycerol
keywords: AQP7; AQP9; Hepatic lipids; Lipid metabolism; Obesity