title: Determination of urinary coenzyme Q10 by HPLC with electrochemical detection: Reference values for a paediatric population.
abstract: Kidney dysfunction is being increasingly associated with mitochondrial diseases and coenzyme Q10 (CoQ) deficiency. The assessment of CoQ status requires the biochemical determination of CoQ in biological fluids and different cell types, but no methods have been developed as yet for the analysis of CoQ in excretory systems. The aim of this study was to standardize a new procedure for urinary CoQ determination and to establish reference values for a paediatric population. Urinary CoQ was analyzed by HPLC with electrochemical detection. Reference values (n = 43) were stratified into two age groups (2-10 years: range 24-109 nmol CoQ/gram of pellet protein; 11-17 years: range 43-139 nmol CoQ/gram of pellet protein). In conclusion, urinary CoQ analysis is a noninvasive, reliable, and reproducible method to determine urinary tract CoQ status.
authors.affiliation: Clinical Biochemistry Department, Institut d'Investigació Sanitària Sant Joan De Déu and CIBERER, Barcelona, Spain.
authors.forename: Dèlia
authors.initials: D
authors.lastname: Yubero
authors.affiliation: Clinical Biochemistry Department, Institut d'Investigació Sanitària Sant Joan De Déu and CIBERER, Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Montero
authors.affiliation: Nephrology Department, Hospital Sant Joan De Déu, Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Ramos
authors.affiliation: Neurometabolic Unit, National Hospital for Neurology and Neurosurgery, London, UK.
authors.forename: Viruna
authors.initials: V
authors.lastname: Neergheen
authors.affiliation: Cellular Biology and Biotechnology Department, Centro Andaluz De Biología Del Desarrollo, Universidad Pablo De Olavide and CIBERER, Sevilla, Spain.
authors.forename: Plácido
authors.initials: P
authors.lastname: Navas
authors.affiliation: Clinical Biochemistry Department, Institut d'Investigació Sanitària Sant Joan De Déu and CIBERER, Barcelona, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Artuch
authors.affiliation: Neurometabolic Unit, National Hospital for Neurology and Neurosurgery, London, UK.
authors.forename: Iain
authors.initials: I
authors.lastname: Hargreaves
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques; D005260:Female; D006801:Humans; D008297:Male; D028361:Mitochondrial Diseases / Q000473:pathology / Q000652:urine*; D051437:Renal Insufficiency / Q000473:pathology / Q000652:urine*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000652:urine
chemical_list: D014451:Ubiquinone; C024989:coenzyme Q10
keywords: coenzyme Q10; high pressure liquid chromatography; mitochondrial disease; reference values; renal disease; urine