title: Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques.
abstract: This work reports the effect of silver bionanoparticles (Bio(AgNPs) synthesized by Actinobacteria CGG 11n on selected Gram (+) and Gram (-) bacteria. Flow cytometry, classical antibiogram method and fluorescent microscopy approach was used for evaluation of antimicrobial activity of Bio(AgNPs) and their combination with antibiotics. Furthermore, the performed research specified the capacity of flow cytometry method as an alternative to the standard ones and as a complementary method to electromigration techniques. The study showed antibacterial activity of both BioAgNPs and the combination of antibiotics/BioAgNPs against all the tested bacteria strains in comparison with a diffusion, dilution and bioautographic methods. The synergistic effect of antibiotics/BioAgNPs combination (e.g. kanamycin, ampicillin, neomycin and streptomycin) was found to be more notable against Pseudomonas aeruginosa representing a prototype of multi-drug resistant "superbugs" for which effective therapeutic options are very limited.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Viorica
authors.initials: V
authors.lastname: Railean-Plugaru
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Pomastowski
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rafinska
authors.affiliation: Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wypij
authors.affiliation: Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kupczyk
authors.affiliation: Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Dahm
authors.affiliation: Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Jackowski
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D005434:Flow Cytometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Actinobacteria; Antibacterial activity; Flow cytometry; Silver nanoparticles; Synergistic effect