title: Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides.
abstract: DNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Verga
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Welter
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany. Electronic address: andreas.marx@uni-konstanz.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
mesh_terms: D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D021881:DNA, Catalytic / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D009841:Oligonucleotides; D001710:Biotin; D004247:DNA; D004259:DNA-Directed DNA Polymerase
keywords: DNA polymerase; Naked-eye detection; Oligonucleotide-modified nucleotide; Rolling circle amplification; Single-base resolution