title: Rice terpene synthase 24 (OsTPS24) encodes a jasmonate-responsive monoterpene synthase that produces an antibacterial γ-terpinene against rice pathogen.
abstract: Rice is one of the most important crops worldwide and is widely used as a model plant for molecular studies of monocotyledonous species. The plant hormone jasmonic acid (JA) is involved in rice-pathogen interactions. In addition, volatile compounds, including terpenes, whose production is induced by JA, are known to be involved in the rice defense system. In this study, we analyzed the JA-induced terpene synthase OsTPS24 in rice. We found that OsTPS24 was localized in chloroplasts and produced a monoterpene, γ-terpinene. The amount of γ-terpinene increased after JA treatment. γ-Terpinene had significant antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo); however, it did not show significant antifungal activity against Magnaporthe oryzae. The antibacterial activity of the γ-terpinene against Xoo was caused by damage to bacterial cell membranes. These results suggest that γ-terpinene plays an important role in JA-induced resistance against Xoo, and that it functions as an antibacterial compound in rice.
authors.affiliation: Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshitomi
authors.affiliation: Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Shiduku
authors.initials: S
authors.lastname: Taniguchi
authors.affiliation: Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Uji
authors.affiliation: Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Akimitsu
authors.affiliation: Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan. Electronic address: gomiken@ag.kagawa-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Gomi
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002849:Chromatography, Gas; D000081005:Cyclohexane Monoterpenes; D003517:Cyclopentanes / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D039821:Monoterpenes / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology*; D054883:Oxylipins / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014974:Xanthomonas / Q000187:drug effects / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D000081005:Cyclohexane Monoterpenes; D003517:Cyclopentanes; D039821:Monoterpenes; D054883:Oxylipins; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; C018669:gamma-terpinene; C011006:jasmonic acid; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
keywords: Jasmonic acid; Plant volatile compounds; Rice; Terpene synthase; γ-Terpinene