title: Non-periodic oscillatory deformation of an actomyosin microdroplet encapsulated within a lipid interface.
abstract: Active force generation in living organisms, which is mainly involved in actin cytoskeleton and myosin molecular motors, plays a crucial role in various biological processes. Although the contractile properties of actomyosin have been extensively investigated, their dynamic contribution to a deformable membrane remains unclear because of the cellular complexities and the difficulties associated with in vitro reconstitution. Here, by overcoming these experimental difficulties, we demonstrate the dynamic deformation of a reconstituted lipid interface coupled with self-organized structure of contractile actomyosin. Therein, the lipid interface repeatedly oscillates without any remarkable periods. The oscillatory deformation of the interface is caused by the aster-like three-dimensional hierarchical structure of actomyosin inside the droplet, which is revealed that the oscillation occurs stochastically as a Poisson process.
authors.affiliation: Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Nishigami
authors.affiliation: Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ito
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 678-1297, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Sonobe
authors.affiliation: Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ichikawa
mesh_terms: D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism*; D001696:Biomechanical Phenomena; D066292:Lipid Droplets / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D010507:Periodicity; D010725:Phosphocreatine / Q000378:metabolism; D014867:Water
chemical_list: D000199:Actins; D008055:Lipids; D010725:Phosphocreatine; D014867:Water; D000205:Actomyosin