title: Chromatin structure and gene regulation: a dynamic view of enhancer function.
abstract: Localized chromatin organization is now recognized as an important determinant of cell identity and developmental pathways. Recent studies have demonstrated that these epigenetic states are unexpectedly dynamic and malleable. In this Extra view we will highlight the transient nature of stimulus-induced enhancer accessibility and its importance for transcription regulation. Using glucocorticoid receptor (GR) as a model system we will discuss spatiotemporal relationships between receptor/chromatin interactions, lifetimes of the DNase I hypersensitivity sites (DHSs), long-range interactions, and gene regulation. We propose that differential temporal activation and utilization of distal regulatory elements plays a role in directing divergent stimulus-induced transcriptional programs.
authors.affiliation: a Laboratory of Receptor Biology and Gene Expression; National Cancer Institute; National Institutes of Health ; Bethesda , MD USA.
authors.forename: Diana A
authors.initials: DA
authors.lastname: Stavreva
authors.affiliation: a Laboratory of Receptor Biology and Gene Expression; National Cancer Institute; National Institutes of Health ; Bethesda , MD USA.
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Hager
mesh_terms: D000818:Animals; D001665:Binding Sites; D064113:CRISPR-Cas Systems / Q000235:genetics; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D008958:Models, Molecular; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
chemical_list: D002843:Chromatin; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors; D012313:RNA; D003850:Deoxyribonuclease I
keywords: DNase I; chromatin accessibility; enhancers; gene pulsing; glucocorticoid receptor; long-range interactions; transcription; ultradian