title: Allergenic Characterization of 27-kDa Glycoprotein, a Novel Heat Stable Allergen, from the Pupa of Silkworm, Bombyx mori.
abstract: Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase, has been identified. The purpose of this study was to identify novel food allergens in silkworm pupa by analyzing a protein extract after heat treatment. Heat treated extracts were examined by proteomic analysis. A 27-kDa glycoprotein was identified, expressed in Escherichia coli, and purified. IgE reactivity of the recombinant protein was investigated by ELISA. High molecular weight proteins (above 100 kDa) elicited increased IgE binding after heat treatment compared to that before heat treatment. The molecular identities of these proteins, however, could not be determined. IgE reactivity toward a 27-kDa glycoprotein was also increased after heating the protein extract. The recombinant protein was recognized by IgE antibodies from allergic subjects (33.3%). Glycation or aggregation of protein by heating may create new IgE binding epitopes. Heat stable allergens are shown to be important in silkworm allergy. Sensitization to the 27-kDa glycoprotein from silkworm may contribute to elevation of IgE to silkworm.
authors.affiliation: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Kyoung Yong
authors.identifier: 0000-0001-9887-1426
authors.initials: KY
authors.lastname: Jeong
authors.affiliation: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: June Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Kyung Hee
authors.identifier: 0000-0003-3605-5364
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jae-Hyun
authors.identifier: 0000-0002-0760-0071
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jung-Won
authors.identifier: 0000-0003-0249-8749
authors.initials: JW
authors.lastname: Park
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000209:etiology; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D006358:Hot Temperature; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D040901:Proteomics; D011679:Pupa / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D016415:Sequence Alignment
chemical_list: D000485:Allergens; D000939:Epitopes; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E
keywords: 27-kDa Glycoprotein; Allergens; Bombyx mori; Hemolymph