title: Caffeine and REM sleep deprivation: Effect on basal levels of signaling molecules in area CA1.
abstract: We have investigated the neuroprotective effect of chronic caffeine treatment on basal levels of memory-related signaling molecules in area CA1 of sleep-deprived rats. Animals in the caffeine groups were treated with caffeine in drinking water (0.3g/l) for four weeks before they were REM sleep-deprived for 24h in the Modified Multiple Platforms paradigm. Western blot analysis of basal protein levels of plasticity- and memory-related signaling molecules in hippocampal area CA1 showed significant down regulation of the basal levels of phosphorylated- and total-CaMKII, phosphorylated- and total-CREB as well as those of BDNF and CaMKIV in sleep deprived rats. All these changes were completely prevented in rats that chronically consumed caffeine. The present findings suggest an important neuroprotective property of caffeine in sleep deprivation.
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, TX, USA. Electronic address: kalkadhi@uh.edu.
authors.forename: Karim A
authors.initials: KA
authors.lastname: Alkadhi
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, TX, USA.
authors.forename: Ibrahim A
authors.initials: IA
authors.lastname: Alhaider
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002110:Caffeine / Q000494:pharmacology* / Q000627:therapeutic use; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism; D000697:Central Nervous System Stimulants / Q000494:pharmacology* / Q000627:therapeutic use; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D012892:Sleep Deprivation / Q000188:drug therapy / Q000378:metabolism*; D012895:Sleep, REM / Q000187:drug effects*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D000697:Central Nervous System Stimulants; D017362:Cyclic AMP Response Element-Binding Protein; D018696:Neuroprotective Agents; D002110:Caffeine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: BDNF; CREB; Immunoblot analysis; Modified multiple platforms; Rat