title: Adenosine plasma level correlates with homocysteine and uric acid concentrations in patients with coronary artery disease.
abstract: The role of hyperhomocysteinemia in coronary artery disease (CAD) patients remains unclear. The present study evaluated the relationship between homocysteine (HCys), adenosine plasma concentration (APC), plasma uric acid, and CAD severity evaluated using the SYNTAX score. We also evaluated in vitro the influence of adenosine on HCys production by hepatoma cultured cells (HuH7). Seventy-eight patients (mean age ± SD: 66.3 ± 11.3; mean SYNTAX score: 19.9 ± 12.3) and 30 healthy subjects (mean age: 61 ± 13) were included. We incubated HuH7 cells with increasing concentrations of adenosine and addressed the effect on HCys level in cell culture supernatant. Patients vs. controls had higher APC (0.82 ± 0.5 μmol/L vs 0.53 ± 0.14 μmol/L; p < 0.01), HCys (15 ± 7.6 μmol/L vs 6.8 ± 3 μmol/L, p < 0.0001), and uric acid (242.6 ± 97 vs 202 ± 59, p < 0.05) levels. APC was correlated with HCys and uric acid concentrations in patients (Pearson's R = 0.65 and 0.52; p < 0.0001, respectively). The SYNTAX score was correlated with HCys concentration. Adenosine induced a time- and dose-dependent increase in HCys in cell culture. Our data suggest that high APC is associated with HCys and uric acid concentrations in CAD patients. Whether the increased APC participates in atherosclerosis or, conversely, is part of a protective regulation process needs further investigations.
authors.affiliation: a UMR MD2, Aix Marseille University and IRBA (Institute of Research in Biology of the French Army), School of Medicine, Bvd P Dramard 13015 Marseille, France.|b Laboratory of Biochemistry, Timone University Hospital, Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Fromonot
authors.affiliation: c Department of Cardiology, Timone University Hospital, Marseille, France.
authors.forename: P
authors.initials: P
authors.lastname: Deharo
authors.affiliation: a UMR MD2, Aix Marseille University and IRBA (Institute of Research in Biology of the French Army), School of Medicine, Bvd P Dramard 13015 Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Bruzzese
authors.affiliation: c Department of Cardiology, Timone University Hospital, Marseille, France.
authors.forename: T
authors.initials: T
authors.lastname: Cuisset
authors.affiliation: c Department of Cardiology, Timone University Hospital, Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Quilici
authors.affiliation: d Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II Napoly", Italia.
authors.forename: S
authors.initials: S
authors.lastname: Bonatti
authors.affiliation: a UMR MD2, Aix Marseille University and IRBA (Institute of Research in Biology of the French Army), School of Medicine, Bvd P Dramard 13015 Marseille, France.
authors.forename: E
authors.initials: E
authors.lastname: Fenouillet
authors.affiliation: a UMR MD2, Aix Marseille University and IRBA (Institute of Research in Biology of the French Army), School of Medicine, Bvd P Dramard 13015 Marseille, France.|b Laboratory of Biochemistry, Timone University Hospital, Marseille, France.
authors.forename: G
authors.initials: G
authors.lastname: Mottola
authors.affiliation: a UMR MD2, Aix Marseille University and IRBA (Institute of Research in Biology of the French Army), School of Medicine, Bvd P Dramard 13015 Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Ruf
authors.affiliation: a UMR MD2, Aix Marseille University and IRBA (Institute of Research in Biology of the French Army), School of Medicine, Bvd P Dramard 13015 Marseille, France.|b Laboratory of Biochemistry, Timone University Hospital, Marseille, France.
authors.forename: R
authors.initials: R
authors.lastname: Guieu
mesh_terms: D000241:Adenosine / Q000097:blood*; D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood*; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D020138:Hyperhomocysteinemia / Q000097:blood; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D014527:Uric Acid / Q000097:blood*
chemical_list: D006710:Homocysteine; D014527:Uric Acid; D000241:Adenosine
keywords: adenosine; adénosine; coronaropathie; coronary artery disease; homocysteine; homocystéine