title: Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer.
abstract: Extracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prostate cancer (PCa) patients exhibit genuine and differential physical and biological properties compared to benign prostate hyperplasia (BPH). Importantly, transcriptomics characterization of uEVs led us to define the decreased abundance of Cadherin 3, type 1 (CDH3) transcript in uEV from PCa patients. Tissue and cell line analysis strongly suggested that the status of CDH3 in uEVs is a distal reflection of changes in the expression of this cadherin in the prostate tumor. CDH3 was negatively regulated at the genomic, transcriptional, and epigenetic level in PCa. Our results reveal that uEVs could represent a non-invasive tool to inform about the molecular alterations in PCa.
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (Ciberehd), Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Royo
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Zuñiga-Garcia
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Torrano
authors.affiliation: Department of Urology, Basurto University Hospital, Bilbao, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Loizaga
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Sanchez-Mosquera
authors.affiliation: Department of Pathology, Basurto University Hospital, Bilbao, Spain.
authors.forename: Aitziber
authors.initials: A
authors.lastname: Ugalde-Olano
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Esperanza
authors.initials: E
authors.lastname: González
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Cortazar
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Palomo
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Fernández-Ruiz
authors.affiliation: Department of Urology, Basurto University Hospital, Bilbao, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Lacasa-Viscasillas
authors.affiliation: Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Berdasco
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: James D
authors.initials: JD
authors.lastname: Sutherland
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Barrio
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Zabala-Letona
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Martín-Martín
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Arruabarrena-Aristorena
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Lorea
authors.initials: L
authors.lastname: Valcarcel-Jimenez
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Caro-Maldonado
authors.affiliation: Department of Urology, Basurto University Hospital, Bilbao, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Gonzalez-Tampan
authors.affiliation: Department of Urology, Basurto University Hospital, Bilbao, Spain.
authors.forename: Guido
authors.initials: G
authors.lastname: Cachi-Fuentes
authors.affiliation: Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain.
authors.forename: Manel
authors.initials: M
authors.lastname: Esteller
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (Ciberehd), Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Aransay
authors.affiliation: Department of Urology, Basurto University Hospital, Bilbao, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Unda
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (Ciberehd), Spain.|Ikerbasque, Basque Foundation for Science, Bizkaia, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Falcón-Pérez
authors.affiliation: CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain.|Ikerbasque, Basque Foundation for Science, Bizkaia, Spain.|Biochemistry and Molecular Biology Department, University of the Basque Country (UPV/EHU), Bizkaia, Spain.
authors.forename: Arkaitz
authors.initials: A
authors.lastname: Carracedo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000652:urine*; D015820:Cadherins / Q000235:genetics* / Q000652:urine*; D055354:Exosomes / Q000378:metabolism; D000067128:Extracellular Vesicles / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000652:urine*
chemical_list: D014408:Biomarkers, Tumor; C512197:CDH3 protein, human; D015820:Cadherins
keywords: exosomes; extracellular vesicles; prostate cancer; urine biomakers