title: Near-infrared fluorescence-based multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk.
abstract: In this study, we developed a novel near-infrared fluorescence based multiplex lateral flow immunoassay by conjugating a near-infrared label to broad-specificity monoclonal antibody/receptor as detection complexes. Different antigens were dispensed onto separate test zones of nitrocellulose membrane to serve as capture reagents. This assay format allowed the simultaneous detection of four families of antibiotics (β-lactams, tetracyclines, quinolones and sulfonamides) in milk within 20 min. Qualitative and quantitative analysis of target antibiotics were realized by imaging the fluorescence intensity of the near-infrared label captured on respective test lines. For qualitative analysis, the cut-off values of β-lactams, tetracyclines, quinolones and sulfonamides were determined to be 8 ng/mL, 2 ng/mL, 4 ng/mL and 8 ng/mL respectively, which were much lower than the conventional gold nanoparticle based lateral flow immunoassay. For quantitative analysis, the detection ranges were 0.26-3.56 ng/mL for β-lactams, 0.04-0.98 ng/mL for tetracyclines, 0.08-2.0 ng/mL for quinolones, and 0.1-3.98 ng/mL for sulfonamides, with linear correlation coefficients higher than 0.97. The mean spiked recoveries ranged from 93.7% to 108.2% with coefficient of variations less than 16.3%. These results demonstrated that this novel immunoassay is a promising approach for rapidly screening the four families of antibiotic residues in milk.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: yqchen@cau.edu.cn.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cancer Genetics and Epigenetics, City of Hope Cancer Center, 1500 East Duarte Road, Duarte, CA 91010, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Han
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Jiangyang
authors.initials: J
authors.lastname: Liu
authors.affiliation: University of Missouri Informatics Institute (MUII), 241 Engineering Building West, Columbia, MO 65211, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390, USA.
authors.forename: Lidong
authors.initials: L
authors.lastname: He
authors.affiliation: China Institute of Veterinary Drug Control, Beijing 110000, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: zhangliying01@sina.com.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D004350:Drug Residues / Q000737:chemistry / Q000302:isolation & purification*; D005453:Fluorescence; D005455:Fluorescent Antibody Technique / Q000379:methods; D005506:Food Contamination; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents
keywords: Lateral flow immunoassay; Near-infrared fluorescence; Quinolones; Sulfonamides; Tetracyclines; β-lactams