title: The Epigenetic Reprogramming Roadmap in Generation of iPSCs from Somatic Cells.
abstract: Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is a comprehensive epigenetic process involving genome-wide modifications of histones and DNA methylation. This process is often incomplete, which subsequently affects iPSC reprogramming, pluripotency, and differentiation capacity. Here, we review the epigenetic changes with a focus on histone modification (methylation and acetylation) and DNA modification (methylation) during iPSC induction. We look at changes in specific epigenetic signatures, aberrations and epigenetic memory during reprogramming and small molecules influencing the epigenetic reprogramming of somatic cells. Finally, we discuss how to improve iPSC generation and pluripotency through epigenetic manipulations.
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus C 8000, Denmark.
authors.forename: Jacob
authors.initials: J
authors.lastname: Brix
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus C 8000, Denmark.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus C 8000, Denmark. Electronic address: alun@biomed.au.dk.
authors.forename: Yonglun
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming; D019175:DNA Methylation; D057890:Epigenomics; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D006657:Histones
keywords: DNA methylation; Epigenetic memory; Epigenetics; Histone modification; Induced pluripotent stem cells (iPSCs); Reprogramming