title: Expression, purification, crystallization and crystallographic analysis of the N-terminal domain of translocated intimin receptor.
abstract: Translocated intimin receptor (Tir) is an Escherichia coli-encoded protein that is transported into the host cell through a sophisticated bacterial type III secretion system (T3SS). Tir anchors the infected cell membrane twice using both its N- and C-termini from inside the host cytoplasm for signalling. It plays a key role in enterohemorrhagic Escherichia coli (EHEC) infection, attaching and effacing (A/E) lesions and intracellular signal transduction. Here, the overexpression, purification and crystallization of its N-terminal intracellular domain are reported. The crystal belonged to the orthorhombic space group I4122, with unit-cell parameters a = b = 59.79, c = 183.11 Å. The asymmetric unit contained one molecule, with a solvent content of 51% and a VM of 2.55 Å(3) Da(-1).
authors.affiliation: Department of Cardiothoracic Surgery, The 306 Hospital, No. 9 Anxiang North Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Bing Yang
authors.initials: BY
authors.lastname: Huang
authors.affiliation: Third Military Medical University, Shapingba District, Chongqing 400038, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Yan Fang
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, The 306 Hospital, No. 9 Anxiang North Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Jun Jun
authors.initials: JJ
authors.lastname: Zhou
authors.affiliation: Department of Cardiothoracic Surgery, The 306 Hospital, No. 9 Anxiang North Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Xiao Yong
authors.initials: XY
authors.lastname: Song
authors.affiliation: Department of Cardiothoracic Surgery, The 306 Hospital, No. 9 Anxiang North Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Cardiothoracic Surgery, The 306 Hospital, No. 9 Anxiang North Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Xin Min
authors.initials: XM
authors.lastname: Li
authors.affiliation: Department of Cardiothoracic Surgery, The 306 Hospital, No. 9 Anxiang North Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D000072417:Protein Domains; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000069296:Type III Secretion Systems
chemical_list: D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; D000069296:Type III Secretion Systems
keywords: N-terminus of Tir; enterohaemorrhagic Escherichia coli; translocated intimin receptor; type III secretion system