title: Optimization of norovirus virus-like particle production in Pichia pastoris using a real-time near-infrared bioprocess monitor.
abstract: The production of norovirus virus-like particles (NoV VLPs) displaying NY-ESO-1 cancer testis antigen in Pichia pastoris BG11 Mut(+) has been enhanced through feed-strategy optimization using a near-infrared bioprocess monitor (RTBio(®) Bioprocess Monitor, ASL Analytical, Inc.), capable of monitoring and controlling the concentrations of glycerol and methanol in real-time. The production of NoV VLPs displaying NY-ESO-1 in P. pastoris has potential as a novel cancer vaccine platform. Optimization of the growth conditions resulted in an almost two-fold increase in the expression levels in the fermentation supernatant of P. pastoris as compared to the starting conditions. We investigated the effect of methanol concentration, batch phase time, and batch to induction transition on NoV VLP-NY-ESO-1 production. The optimized process included a glycerol transition phase during the first 2 h of induction and a methanol concentration set point of 4 g L(-1) during induction. Utilizing the bioprocess monitor to control the glycerol and methanol concentrations during induction resulted in a maximum NoV VP1-NY-ESO-1 yield of 0.85 g L(-1) . © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:518-526, 2016.
authors.affiliation: Dept. of Biomedical Engineering, Cornell University, 357 Stocking Hall Ithaca, NY 14853, Ithaca, NY.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Parker
authors.affiliation: Dept. of Biological Engineering, Cornell University, Ithaca, NY.
authors.forename: Mitchell H
authors.initials: MH
authors.lastname: Maloy
authors.affiliation: Dept. of Microbiology, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Jaime
authors.initials: J
authors.lastname: Tome-Amat
authors.affiliation: Dept. of Food Science, Cornell University, Ithaca, NY.
authors.forename: Cameron L
authors.initials: CL
authors.lastname: Bardliving
authors.affiliation: Dept. of Food Science, Cornell University, Ithaca, NY.
authors.forename: Carl A
authors.initials: CA
authors.lastname: Batt
authors.affiliation: ASL Analytical, Inc, 2500 Crosspark Rd, Coralville, IA.
authors.forename: Kaylee J
authors.initials: KJ
authors.lastname: Lanz
authors.affiliation: ASL Analytical, Inc, 2500 Crosspark Rd, Coralville, IA.
authors.forename: Jonathon T
authors.initials: JT
authors.lastname: Olesberg
authors.affiliation: ASL Analytical, Inc, 2500 Crosspark Rd, Coralville, IA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Arnold
mesh_terms: D005285:Fermentation; D005990:Glycerol / Q000032:analysis / Q000378:metabolism; D000432:Methanol / Q000032:analysis / Q000378:metabolism; D029322:Norovirus / Q000737:chemistry / Q000378:metabolism*; D010316:Particle Size; D010843:Pichia / Q000737:chemistry* / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
chemical_list: D005990:Glycerol; D000432:Methanol
keywords: NY-ESO-1; Norovirus virus-like particles; bioprocess monitor; fed-batch fermentation