title: Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions.
abstract: Sorghum is one of the world's most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum's growing season and increases yield in temperate regions. However, sorghum's sensitivity to low soil temperatures adversely impacts seed germination. In this study, we evaluated the 242 accessions of the ICRISAT sorghum mini core collection for seed germination and seedling vigor at 12 °C as a measure of cold tolerance. Genome-wide association analysis was performed with approximately 162,177 single nucleotide polymorphism markers. Only one marker locus (Locus 7-2) was significantly associated with low-temperature germination and none with vigor. The linkage of Locus 7-2 to low-temperature germination was supported by four lines of evidence: strong association in three independent experiments, co-localization with previously mapped cold tolerance quantitative trait loci (QTL) in sorghum, a candidate gene that increases cold tolerance and germination rate when its wheat homolog is overexpressed in tobacco, and its syntenic region in rice co-localized with two cold tolerance QTL in rice. This locus may be useful in developing tools for molecular breeding of sorghums with improved low-temperature germinability.
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India.|b Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.|c UWA Institute of Agriculture, the University of Western Australia, Crawley WA 6009, Australia.
authors.forename: Hari D
authors.initials: HD
authors.lastname: Upadhyaya
authors.affiliation: d Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India.
authors.forename: Dintyala V S S R
authors.initials: DV
authors.lastname: Sastry
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India.
authors.forename: Sangam L
authors.initials: SL
authors.lastname: Dwivedi
authors.affiliation: b Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: P V Vara
authors.initials: PV
authors.lastname: Prasad
authors.affiliation: e Department of Horticulture and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA.
authors.forename: A Millie
authors.initials: AM
authors.lastname: Burrell
authors.affiliation: f USDA-ARS, Southern Plains Agricultural Research Center, College Station, TX 77845, USA.
authors.forename: Robert R
authors.initials: RR
authors.lastname: Klein
authors.affiliation: b Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Geoffrey P
authors.initials: GP
authors.lastname: Morris
authors.affiliation: e Department of Horticulture and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Klein
mesh_terms: D000222:Adaptation, Physiological; D002874:Chromosome Mapping; D003080:Cold Temperature; D017343:Genes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D018525:Germination / Q000235:genetics*; D058977:Molecular Sequence Annotation; D012275:Oryza / Q000235:genetics; D012621:Seasons; D036226:Seedlings / Q000235:genetics* / Q000254:growth & development; D045868:Sorghum / Q000235:genetics* / Q000254:growth & development; D026801:Synteny
keywords: SNP; association mapping; basses températures en début de saison; cartographie par association pangénomique; early-season cold tolerance; sorgho; sorghum