title: Helicobacter pullorum induces nitric oxide release in murine macrophages that promotes phagocytosis and killing.
abstract: Helicobacter pullorum is an avian enterohepatic species that, more recently, has also been found as a naturally acquired infection in mice and rats, and isolated from patients with gastrointestinal and hepatobiliary diseases. In this work, the interaction between H. pullorum and murine macrophages was examined. Firstly, the impact of nitric oxide, which is an antimicrobial produced by mammalian macrophages, on H. pullorum 6350-92 viability and morphology was studied by colony-forming assays and light microscopy, respectively. Exposure to nitric oxide lowered H. pullorum viability, in a growth-phase-dependent manner, and decreased the mean cell size. However, the number of coccoid forms remained low, contrasting with what has been observed for other Helicobacter species. Confocal microscopy showed that H. pullorum is internalized by murine macrophages, triggering nitric oxide production that promotes phagocytosis and killing of the pathogen. Interaction between H. pullorum and macrophages stimulated secretion of pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-6 and MIP-2. These results show that H. pullorum is able to infect mammalian murine cells triggering an inflammatory response.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República EAN, 2780-157, Oeiras, Portugal.
authors.forename: Margarida R
authors.initials: MR
authors.lastname: Parente
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República EAN, 2780-157, Oeiras, Portugal.
authors.forename: João T
authors.initials: JT
authors.lastname: Monteiro
authors.affiliation: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156, Oeiras, Portugal.
authors.forename: Gabriel G
authors.initials: GG
authors.lastname: Martins
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República EAN, 2780-157, Oeiras, Portugal.
authors.forename: Lígia M
authors.initials: LM
authors.lastname: Saraiva
mesh_terms: D000818:Animals; D002460:Cell Line; D015169:Colony Count, Microbial; D016207:Cytokines / Q000378:metabolism; D016998:Helicobacter / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects*; D008853:Microscopy; D009569:Nitric Oxide / Q000378:metabolism*; D010587:Phagocytosis
chemical_list: D016207:Cytokines; D009569:Nitric Oxide