title: The effect of the paraoxonase 1 (PON1) T(-107)C polymorphism on serum PON1 activity in women is dependent on fatty acid intake.
abstract: Paraoxonase 1 (PON1) is an enzyme that prevents the peroxidation of lipoprotein and cell membranes. Our hypothesis is that the effect of the PON1 T(-107)C polymorphism on serum PON1 activity in healthy adult women is dependent on their fatty acid intake profile. This study included women (n = 39) who completed a food frequency questionnaire. Fatty acid intake was estimated based on the interview and a nutrient reference table. Blood samples were collected for genotyping and to measure serum PON1 activity. Serum PON1 activity was different among genotypes and was higher for women of the CC genotype (P < .001). Women in the study were categorized in 2 groups according to the median nutrient intake. Overall, there was a difference (P < .05) in serum PON1 activity between the CC and TT genotypes in women ingesting either above or below the median total fat, saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, omega 3 (n-3) and omega 6 (n-6; P < .05). However, genotype effects on serum PON1 activity were not observed in women ingesting below the median (15:1) ratio of n-6/n-3 (P > .05) but were observed in women ingesting above the ratio of n-6/n-3 (P < .05). This is partly because women of the CC genotype had decreased PON1 activity when ingesting a lower ratio of n-6/n-3 diet (P < .05), while women of the TT genotype had increased PON1 activity (P < .05). In conclusion, the overall presence of the C allele was associated with increased serum PON1 activity, although a diet with high saturated fatty acid or a low ratio of n-6/n-3 reduced PON1 activity in women with the CC genotype.
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Fabiola G
authors.initials: FG
authors.lastname: Santos
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Maitê K
authors.initials: MK
authors.lastname: Becker
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Corrêa
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Driele N
authors.initials: DN
authors.lastname: Garcia
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Sandra C
authors.initials: SC
authors.lastname: Vale
authors.affiliation: Department of Gynecology and Obstetrics, College of Medicine, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: José A
authors.initials: JA
authors.lastname: Crespo-Ribeiro
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Carlos C
authors.initials: CC
authors.lastname: Barros
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Electronic address: augusto.schneider@ufpel.edu.br.
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000293:Adolescent; D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D001938:Brazil; D003430:Cross-Sectional Studies; D003677:Deficiency Diseases / Q000097:blood / Q000201:enzymology / Q000209:etiology / Q000235:genetics; D059305:Diet, High-Fat / Q000009:adverse effects*; D005228:Fatty Acids, Essential / Q000172:deficiency; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000009:adverse effects; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage* / Q000009:adverse effects / Q000172:deficiency; D005260:Female; D056726:Genetic Association Studies; D006785:Hospitals, University; D006801:Humans; D015227:Lipid Peroxidation; D008875:Middle Aged; D009749:Nutrition Surveys; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: D005228:Fatty Acids, Essential; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
keywords: Human; SNPs; Unsaturated fat; omega-3 Fatty acids; omega-6 Fatty acids