title: Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography.
abstract: The effects of 5, 20, and 40 miracidia dose exposures of Echinostoma caproni on the amino acid contents of Biomphalaria glabrata were studied using high performance thin-layer chromatography-densitometry. Amino acids were identified and quantified in whole bodies of exposed snails and in the uninfected matched controls at 2 and 4 weeks post-exposure. Using cellulose layers with the mobile phase 2-butanol-pyridine-glacial acetic acid-deionized water (39:34:10:26) and ninhydrin detection reagent [2% ninhydrin in acetone-n-butanol (1:1)], five amino acids were identified, i.e., leucine/isoleucine, valine, alanine, glycine, and ornithine, by hRF value comparison and color differentiation. Quantitatively, there was a marked elevation in the amounts of four of these five amino acids (isoleucine/leucine, valine, alanine, and ornithine) across dose levels at 4 weeks post-infection (P<0.05). Elevation of the amino acid content in the high dose snail group suggested that some changes occurred in the amino acid metabolism of the snails in that group as a function of miracidia dose.
authors.forename: Mai
authors.initials: M
authors.lastname: Nguyen
authors.forename: Bernard
authors.initials: B
authors.lastname: Fried
authors.forename: Joseph
authors.initials: J
authors.lastname: Sherma
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001700:Biomphalaria / Q000737:chemistry* / Q000469:parasitology*; D002855:Chromatography, Thin Layer; D004449:Echinostoma / Q000254:growth & development*; D059208:Parasite Load
chemical_list: D000596:Amino Acids