title: Remifentanil-induced preconditioning has cross-talk with A1 and A2B adenosine receptors in ischemic-reperfused rat heart.
abstract: The purpose of this study was to determine whether there is a cross-talk between opioid receptors (OPRs) and adenosine receptors (ADRs) in remifentanil preconditioning (R-Pre) and, if so, to investigate the types of ADRs involved in the cross-talk. Isolated rat hearts received 30 min of regional ischemia followed by 2 hr of reperfusion. OPR and ADR antagonists were perfused from 10 min before R-Pre until the end of R-Pre. The heart rate, left ventricular developed pressure (LVDP),velocity of contraction (+dP/dtmax), and coronary flow (CF) were recorded. The area at risk and area of necrosis were measured. After reperfusion, the LVDP, +dP/dtmax,and CF showed a significant increase in the R-Pre group compared with the control group (no intervention before or after regional ischemia). These increases in the R-Pre group were blocked by naloxone, a nonspecific ADR antagonist, an A1 ADR antagonist, and an A2B ADR antagonist. The infarct size was reduced significantly in the R-Pre group compared with the control group. The infarct-reducing effect in the R-Pre group was blocked by naloxone, the nonspecific ADR antagonist, the A1 ADR antagonist, and the A2B ADR antagonist. The results of this study demonstrate that there is cross-talk between ADRs and OPRs in R-Pre and that A1 ADR and A2B ADR appear to be involved in the cross-talk.
authors.affiliation: Department of Anesthesiology and Pain Medicine, School of Medicine, Keimyung University. yclee@dsmc.or.kr.
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Lee
authors.forename: Jiyoon
authors.initials: J
authors.lastname: Jung
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Park
mesh_terms: D000241:Adenosine / Q000737:chemistry; D000701:Analgesics, Opioid / Q000737:chemistry*; D000818:Animals; D003326:Coronary Circulation; D006321:Heart / Q000502:physiology; D019194:Ischemic Preconditioning; D008297:Male; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000378:metabolism; D010880:Piperidines / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043705:Receptor, Adenosine A2A / Q000235:genetics*; D043704:Receptor, Adenosine A2B / Q000235:genetics*; D011957:Receptors, Opioid / Q000378:metabolism; D000077208:Remifentanil; D015427:Reperfusion Injury / Q000473:pathology*; D013997:Time Factors; D016277:Ventricular Function, Left
chemical_list: D000701:Analgesics, Opioid; D010880:Piperidines; D043705:Receptor, Adenosine A2A; D043704:Receptor, Adenosine A2B; D011957:Receptors, Opioid; D000241:Adenosine; D000077208:Remifentanil