title: Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars.
abstract: P[(R)-lactate-co-(R)-3-hydroxybutyrate] [P(LA-co-3HB)] was produced in engineered Escherichia coli using lignocellulose-derived hydrolysates from Miscanthus × giganteus (hybrid Miscanthus) and rice straw. Hybrid Miscanthus-derived hydrolysate exhibited no negative effect on polymer production, LA fraction, and molecular weight of the polymer, whereas rice straw-derived hydrolysate reduced LA fraction. These results revealed that P(LA-co-3HB) was successfully produced from hybrid Miscanthus-derived sugars.
authors.affiliation: a Division of Applied Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: a Division of Applied Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.
authors.forename: Camila
authors.initials: C
authors.lastname: Utsunomia
authors.affiliation: a Division of Applied Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Sasaki
authors.affiliation: a Division of Applied Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: b Field Science Center for Northern Biosphere, Hokkaido University , Sapporo , Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yamada
authors.affiliation: a Division of Applied Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.|c CREST , Kawaguchi , Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ooi
authors.affiliation: a Division of Applied Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.|c CREST , Kawaguchi , Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D019344:Lactic Acid; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: bio-based polyester; biorefinery; biosynthesis; lignocellulosic biomass