title: Kv1.3 potassium channel mediates macrophage migration in atherosclerosis by regulating ERK activity.
abstract: Ion channels expressed in macrophages have been tightly related to atherosclerosis by coupling cellular function. How the voltage-gated potassium channels (Kv) affect macrophage migration remain unknown. The aim of our study is to investigate whether Kv1.3-ERK signaling pathway plays an important role in the process. We explored the expression of Kv1.3 in coronary atherosclerotic heart disease and found Kv1.3 channel was increased in acute coronary syndrome patients. Treatment of RAW264.7 cells with Kv1.3 small interfering RNA, suppressed cell migration. The expression of phosphorylated ERK1/2 also decreased after knockdown of Kv1.3. On the other hand, overexpression of Kv1.3 channel promoted cell migration and ERK1/2 phosphorylation. U-0126, the mitogen-activated protein kinase inhibitors, could reverse macrophage migration induced by Kv1.3 channel overexpression. Downregulation of Kv1.3 channel by siRNA could not further inhibit cell migration when cells were treated with U-0126. It means that ERK is downstream signal of Kv1.3 channel. We concluded that Kv1.3 may stimulate macrophage migration through the activation of ERK.
authors.affiliation: Department of Geriatric Cardiology, Qilu Hospital of Shandong University, 107 Wenhua Xi Rd, Jinan 250012, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Kan
authors.affiliation: Department of Geriatric Cardiology, Qilu Hospital of Shandong University, 107 Wenhua Xi Rd, Jinan 250012, China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Gao
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Department of Cardiology, QiLu Hospital of Shandong University, 107 Wenhua Xi Rd, Jinan 250012, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Ma
authors.affiliation: Department of Geriatric Cardiology, Qilu Hospital of Shandong University, 107 Wenhua Xi Rd, Jinan 250012, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Geriatric Cardiology, Qilu Hospital of Shandong University, 107 Wenhua Xi Rd, Jinan 250012, China.
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Ling
authors.affiliation: Department of Geriatric Cardiology, Qilu Hospital of Shandong University, 107 Wenhua Xi Rd, Jinan 250012, China. Electronic address: wangyuanyuan1204@163.com.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D003324:Coronary Artery Disease / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D051664:Kv1.3 Potassium Channel / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D000067996:RAW 264.7 Cells
chemical_list: D051664:Kv1.3 Potassium Channel
keywords: Atherosclerosis; Cell migration; Extracellular signal-regulated kinase; Macrophage; Voltage-gated potassium channel