title: Diagnostic performance of the "MESACUP anti-Skin profile TEST".
abstract: BACKGROUND
  The "MESACUP anti-Skin profile TEST" is a new, commercially available ELISA kit to detect circulating IgG autoantibodies against desmoglein 1, desmoglein 3, BP180, BP230, and type VII collagen, both simultaneously and more rapidly than previous assays.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the diagnostic accuracy of this kit for the diagnosis of pemphigus foliaceus, pemphigus vulgaris, bullous pemphigoid and epidermolysis bullosa acquisita.
  
  
  METHODS
  Dual-centre retrospective study in which 138 patients with autoimmune blistering diseases were compared to 40 controls
  
  
  RESULTS
  Using the MESACUP anti-Skin profile TEST, both sensitivities and specificities for desmoglein 1, desmoglein 3, BP180, BP230, and type VII collagen autoantibodies were similar to those obtained using previous, specific ELISA systems and 88% of the results were concordant without any significant difference.
  
  
  CONCLUSIONS
  The MESACUP anti-Skin profile TEST had a similar performance to previously produced ELISA systems. The novel kit can be used for rapid diagnosis of most common autoimmune blistering diseases and is especially suitable for identifying overlapping disorders.
authors.affiliation: Department of Dermatology, Allergology, Ludwig Maximilian University, Frauenlobstr. 9-11, 80337 Munich, Germany, Institute of Behavioural Sciences, Semmelweis University, Budapest, Hungary.
authors.forename: Orsolya N
authors.initials: ON
authors.lastname: Horváth
authors.affiliation: Department of Dermatology, Allergology, Ludwig Maximilian University, Frauenlobstr. 9-11, 80337 Munich, Germany.
authors.forename: Rita
authors.initials: R
authors.lastname: Varga
authors.affiliation: Medical and Biological Laboratories Company, Ltd., Nagoya, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kaneda
authors.affiliation: Department of Dermatology, University of Lübeck, Lübeck, Germany.
authors.forename: Enno
authors.initials: E
authors.lastname: Schmidt
authors.affiliation: Department of Dermatology, Allergology, Ludwig Maximilian University, Frauenlobstr. 9-11, 80337 Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ruzicka
authors.affiliation: Department of Dermatology, Allergology, Ludwig Maximilian University, Frauenlobstr. 9-11, 80337 Munich, Germany.
authors.forename: Miklós
authors.initials: M
authors.lastname: Sárdy
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D024143:Collagen Type VII / Q000276:immunology; D051183:Desmoglein 1 / Q000276:immunology; D051184:Desmoglein 3 / Q000276:immunology; D000071821:Dystonin / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D024041:Non-Fibrillar Collagens / Q000276:immunology; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012867:Skin / Q000276:immunology*; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis* / Q000276:immunology; D000097805:Collagen Type XVII
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D024143:Collagen Type VII; D051183:Desmoglein 1; D051184:Desmoglein 3; D000071821:Dystonin; D007074:Immunoglobulin G; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human
keywords: ELISA; bullous pemphigoid; diagnostics; epidermolysis bullosa acquisita; pemphigus foliaceus; pemphigus vulgaris