title: Population structure of colonizing and invasiveStaphylococcus aureusstrains in northern Vietnam.
abstract: Staphylococcus aureusis an important global health problem worldwide. There is still scarce information on the population structure ofS. aureusstrains in Asia, where the majority of the world population lives. This study characterized the diversity ofS. aureusstrains in northern Vietnam through multilocus sequence typing (MLST). Eighty-five carriage isolates from the community and 77 invasive isolates from the clinical setting were selected and tested for meticillin resistance and the presence of Panton-Valentine leukocidin (PVL). MLST was performed on these isolates, of which CC59 (25.4 %), CC188 (17.3 %) and CC45 (16.7 %) were the predominant clonal complexes (CCs). CC59 carriage isolates had significantly lower rates of meticillin-resistantS. aureus(MRSA) than their corresponding clinical group isolates (32 vs 83 %). There were no significant differences in rates of MRSA between carriage isolates and clinical isolates of CC45 and CC188. CC59 carriage isolates were significantly lower in rates of PVL+than CC59 clinical isolates (32 vs 83 %), but the converse was shown in CC45 isolates (14 vs 0 %, respectively). This study revealed vast differences in the molecular epidemiology and population structure ofS. aureusin community and clinical settings in Vietnam. Nevertheless, the data underline the spread of virulent and/or resistant strains (MRSA and/or PVL+) in the community, suggesting the necessity for further surveillance to determine the mechanism of transmission of these strains (i.e. MRSA/PVL+) outside clinical settings.
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam.
authors.forename: Bich
authors.initials: B
authors.lastname: Ngoc Thi Vu
authors.affiliation: Princeton University, Princeton, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: J Jafari
authors.affiliation: Princeton University, Princeton, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Aardema
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam.
authors.forename: Huong
authors.initials: H
authors.lastname: Kieu Thi Tran
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam.
authors.forename: Diep
authors.initials: D
authors.lastname: Ngoc Thi Nguyen
authors.affiliation: National Hospital for Tropical Diseases, Hanoi, Vietnam.
authors.forename: Trinh
authors.initials: T
authors.lastname: Tuyet Dao
authors.affiliation: National Hospital for Tropical Diseases, Hanoi, Vietnam.
authors.forename: Trung
authors.initials: T
authors.lastname: Vu Nguyen
authors.affiliation: Hanoi Medical University, Hanoi, Vietnam.
authors.forename: Toan
authors.initials: T
authors.lastname: Khanh Tran
authors.affiliation: Hanoi Medical University, Hanoi, Vietnam.
authors.forename: Chuc
authors.initials: C
authors.lastname: Kim Thi Nguyen
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam.
authors.forename: Annette
authors.initials: A
authors.lastname: Fox
authors.affiliation: MIVEGEC unit, IRD 224-CNRS 5290-University of Montpellier, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Bañuls
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam.|Nuffield Department of Clinical Medicine, Centre for Tropical Medicine, University of Oxford, Oxford, UK.
authors.forename: Guy
authors.initials: G
authors.lastname: Thwaites
authors.affiliation: National Hospital for Tropical Diseases, Hanoi, Vietnam.
authors.forename: Kinh
authors.initials: K
authors.lastname: Van Nguyen
authors.affiliation: Department of Medical Microbiology, Radboudumc, Nijmegen, The Netherlands.|Oxford University Clinical Research Unit, Hanoi, Vietnam.|Nuffield Department of Clinical Medicine, Centre for Tropical Medicine, University of Oxford, Oxford, UK.
authors.forename: Heiman F L
authors.initials: HFL
authors.lastname: Wertheim