title: SMN affects membrane remodelling and anchoring of the protein synthesis machinery.
abstract: Disconnection between membrane signalling and actin networks can have catastrophic effects depending on cell size and polarity. The survival motor neuron (SMN) protein is ubiquitously involved in assembly of spliceosomal small nuclear ribonucleoprotein particles. Other SMN functions could, however, affect cellular activities driving asymmetrical cell surface expansions. Genes able to mitigate SMN deficiency operate within pathways in which SMN can act, such as mRNA translation, actin network and endocytosis. Here, we found that SMN accumulates at membrane protrusions during the dynamic rearrangement of the actin filaments. In addition to localization data, we show that SMN interacts with caveolin-1, which mediates anchoring of translation machinery components. Importantly, SMN deficiency depletes the plasma membrane of ribosomes, and this correlates with the failure of fibroblasts to extend membrane protrusions. These findings strongly support a relationship between SMN and membrane dynamics. We propose that SMN could assembly translational platforms associated with and governed by the plasma membrane. This activity could be crucial in cells that have an exacerbated interdependence of membrane remodelling and local protein synthesis.
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy IRCCS Fondazione Santa Lucia, Rome 00143, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Gabanella
authors.affiliation: CNR-IBPM, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Pisani
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy IRCCS Fondazione Santa Lucia, Rome 00143, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Borreca
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy IRCCS Fondazione Santa Lucia, Rome 00143, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Farioli-Vecchioli
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy European Brain Research Institute (EBRI) Rita Levi-Montalcini, Rome 00143, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Ciotti
authors.affiliation: Department of Ecological and Biological Sciences, Tuscia University, Viterbo 01100, Italy.
authors.forename: Tiziano
authors.initials: T
authors.lastname: Ingegnere
authors.affiliation: CNR-IBPM, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Onori
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy IRCCS Fondazione Santa Lucia, Rome 00143, Italy.
authors.forename: Martine
authors.initials: M
authors.lastname: Ammassari-Teule
authors.affiliation: CNR-IBPM, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Corbi
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy Department of System Medicine, University of 'Tor Vergata', Rome 00137, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Canu
authors.affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome 00185, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Monaco
authors.affiliation: CNR-IBPM, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Passananti
authors.affiliation: CNR-Institute of Cell Biology and Neurobiology, Rome 00143, Italy IRCCS Fondazione Santa Lucia, Rome 00143, Italy mariagrazia.dicerto@cnr.it.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Di Certo
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D022081:Cell Surface Extensions / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D006801:Humans; D014176:Protein Biosynthesis; D021381:Protein Transport; D012270:Ribosomes / Q000378:metabolism; D055532:SMN Complex Proteins / Q000502:physiology*
chemical_list: D051242:Caveolin 1; D055532:SMN Complex Proteins
keywords: Actin filament; Local translation; Membrane remodelling; SMN