title: Trypanosoma cruzi Proline Transport Presents a Cell Density-dependent Regulation.
abstract: Trypanosoma cruzi, the etiological agent of Chagas disease, uses proline as its main carbon source, essential for parasite growth and stage differentiation in epimastigotes and amastigotes. Since proline is mainly obtained from extracellular medium by transport proteins, in this work we studied the regulation of the T. cruzi proline transporter TcAAAP069. Proline uptake and intracellular concentration presented oscillations during epimastigote growth phases, increasing during the early exponential phase (322 pmol/min) and decreasing to undetectable levels during the late exponential phase. Transporter expression rate correlated with proline uptake, and its subcellular localization alternated from both, the plasma membrane and close to the flagellar pocket, when the transport is higher, to only the flagellar pocket region, when the transport decreased until proline uptake and TcAAAP069 protein became undetectable at the end of the growth curve. Interestingly, when parasites were treated with conditioned medium or were concentrated to artificially increase the culture density, the proline transport was completely abolished resembling the effects observed in late exponential phase. These data highlight for the first time the existence of a density-associated regulation of relevant physiological processes such as proline metabolism.
authors.affiliation: Laboratorio de Parasitología Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Universidad de Buenos Aires and CONICET, Buenos Aires, Argentina.
authors.forename: Melisa
authors.initials: M
authors.lastname: Sayé
authors.affiliation: Laboratorio de Parasitología Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Universidad de Buenos Aires and CONICET, Buenos Aires, Argentina.
authors.forename: Mariana R
authors.initials: MR
authors.lastname: Miranda
authors.affiliation: Laboratorio de Parasitología Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Universidad de Buenos Aires and CONICET, Buenos Aires, Argentina.
authors.forename: Chantal
authors.initials: C
authors.lastname: Reigada
authors.affiliation: Laboratorio de Parasitología Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Universidad de Buenos Aires and CONICET, Buenos Aires, Argentina.
authors.forename: Claudio A
authors.initials: CA
authors.lastname: Pereira
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D003593:Cytoplasm / Q000737:chemistry; D005786:Gene Expression Regulation; D015388:Organelles; D011392:Proline / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D026921:Amino Acid Transport Systems, Neutral; C033082:proline transporter; D011392:Proline
keywords: Chagas disease; proline uptake; trypanosomatids