title: Methanosarcina flavescenssp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.
abstract: A novel, strictly anaerobic, methanogenic archaeon, strain E03.2T, was isolated from a full-scale biogas plant in Germany. Cells were non-motile sarcina-like cocci, occurring in aggregates. Strain E03.2Tgrew autotrophically on H2plus CO2, and additionally cells could utilize acetate, methanol, moni-, di- and trimethylamine as carbon and energy sources; however, growth or methanogenesis on formate was not observed. Yeast extract and vitamins stimulated growth but were not mandatory. The optimal growth temperature of strain E03.2Twas approximately 45 °C; maximal growth rates were obtained at about pH 7.0 in the presence of approximately 6.8 mM NaCl. The DNA G+C content of strain E03.2Twas 41.3 mol%. Phylogenetic analyses based on 16S rRNA gene andmcrAsequences placed strain E03.2Twithin the genusMethanosarcina. Based on 16S rRNA gene sequence similarity strain E03.2Twas related to seven different species of the genusMethanosarcina, but most closely related toMethanosarcina thermophilaTM-1T. Phenotypic, physiological and genomic characteristics indicated that strain E03.2Trepresents a novel species of the genusMethanosarcina, for which the nameMethanosarcina flavescenssp. nov. is proposed. The type strain is E03.2T( = DSM 100822T= JCM 30921T).
authors.affiliation: Institut für Mikrobiologie, Technische Universität Dresden, 01062 Dresden, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Kern
authors.affiliation: Institut für Allgemeine Mikrobiologie, Universität Kiel, 24118 Kiel, Germany.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Fischer
authors.affiliation: Institut für Mikrobiologie und Biotechnologie, Universität Bonn, 53115 Bonn, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Deppenmeier
authors.affiliation: Institut für Allgemeine Mikrobiologie, Universität Kiel, 24118 Kiel, Germany.
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Schmitz
authors.affiliation: Institut für Mikrobiologie, Technische Universität Dresden, 01062 Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Rother