title: Functional and cell proliferative properties of an exopolysaccharide produced by Nitratireductor sp. PRIM-31.
abstract: Exopolysaccharides (EPS) produced by bacteria are important source of natural biomolecules with therapeutic applications. In this study EPS produced by a strain PRIM-31 isolated from seawater from south west coast of India identified by 16S rRNA gene sequencing as Nitratireductor sp. was investigated for its biotechnological applications. The isolate produced 650 mg L(-1) EPS under optimum growth conditions. The purified EPS contained 58.3% carbohydrates with 7.08% uronic acid containing sugars, functional groups such as sulfate (2.68%) and trace amounts of proteins (0.65%). Molecular weight of the EPS was 90 kDa and monosaccharide composition was arabinose, glucose, xylose, glucuronic acid and galactose (6.6: 3.5: 2.1: 1.3: 1). The EPS displayed antioxidant activity in terms of total antioxidant capacity, ferric reducing power, scavenging of DPPH (IC50 390 μg mL(-1)) and superoxide radicals (IC50 340 μg mL(-1)). Cell proliferative activity of the EPS was evidenced by significant improvement in human dermal fibroblast (HDF) proliferation compared to control. Further, significant improvement (41%) in HDF cell migration was observed in in vitro scratch assay with EPS treatment. The EPS also showed antiproliferative activity against glioblastoma cells with an IC50 of 234.04 μg mL(-1). These functional properties of the EPS prospect its biotechnological applications.
authors.affiliation: Yenepoya Research Centre, Yenepoya University, Mangalore, India.
authors.forename: P
authors.initials: P
authors.lastname: Priyanka
authors.affiliation: Yenepoya Research Centre, Yenepoya University, Mangalore, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Arun
authors.affiliation: Yenepoya Research Centre, Yenepoya University, Mangalore, India.
authors.forename: P
authors.initials: P
authors.lastname: Ashwini
authors.affiliation: Yenepoya Research Centre, Yenepoya University, Mangalore, India. Electronic address: rekhapd@hotmail.com.
authors.forename: P D
authors.initials: PD
authors.lastname: Rekha
mesh_terms: D020561:Alphaproteobacteria / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D011135:Polysaccharides, Bacterial
keywords: Exopolysaccharide; HDF cell proliferation; Wound healing