title: Cytocompatibility, mechanical and dissolution properties of high strength boron and iron oxide phosphate glass fibre reinforced bioresorbable composites.
abstract: In this study, Polylactic acid (PLA)/phosphate glass fibres (PGF) composites were prepared by compression moulding. Fibres produced from phosphate based glasses P2O5-CaO-MgO-Na2O (P45B0), P2O5-CaO-MgO-Na2O-B2O3 (P45B5), P2O5-CaO-MgO-Na2O-Fe2O3 (P45Fe3) and P2O5-CaO-MgO-Na2O-B2O3-Fe2O3 (P45B5Fe3) were used to reinforce the bioresorbable polymer PLA. Fibre mechanical properties and degradation rate were investigated, along with the mechanical properties, degradation and cytocompatibility of the composites. Retention of the mechanical properties of the composites was evaluated during degradation in PBS at 37°C for four weeks. The fibre volume fraction in the composite varied from 19 to 23%. The flexural strength values (ranging from 131 to 184MPa) and modulus values (ranging from 9.95 to 12.29GPa) obtained for the composites matched those of cortical bone. The highest flexural strength (184MPa) and modulus (12.29GPa) were observed for the P45B5Fe3 composite. After 28 days of immersion in PBS at 37°C, ~35% of the strength profile was maintained for P45B0 and P45B5 composites, while for P45Fe3 and P45B5Fe3 composites ~40% of the initial strength was maintained. However, the overall wet mass change of P45Fe3 and P45B5Fe3 remained significantly lower than that of the P45B0 and P45B5 composites. The pH profile also revealed that the P45B0 and P45B5 composites degraded quicker, correlating well with the degradation profile. From SEM analysis, it could be seen that after 28 days of degradation, the fibres in the fractured surface of P45B5Fe3 composites remain fairly intact as compared to the other formulations. The in vitro cell culture studies using MG63 cell lines revealed both P45Fe3 and P45B5Fe3 composites maintained and showed higher cell viability as compared to the P45B0 and P45B5 composites. This was attributed to the slower degradation rate of the fibres in P45Fe3 and P45B5Fe3 composites as compared with the fibres in P45B0 and P45B5 composites.
authors.affiliation: Division of Materials, Mechanics and Structures, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK. Electronic address: nusrat_sharmin27@yahoo.com.
authors.forename: Nusrat
authors.initials: N
authors.lastname: Sharmin
authors.affiliation: Purac Biomaterials, 5150 N Royal Atlanta DR. Tucker, GA 30084, USA.
authors.forename: Muhammad S
authors.initials: MS
authors.lastname: Hasan
authors.affiliation: Division of Materials, Mechanics and Structures, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Parsons
authors.affiliation: Division of Materials, Mechanics and Structures, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK.
authors.forename: Chris D
authors.initials: CD
authors.lastname: Rudd
authors.affiliation: Division of Materials, Mechanics and Structures, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK. Electronic address: Ifty.Ahmed@nottingham.ac.uk.
authors.forename: Ifty
authors.initials: I
authors.lastname: Ahmed
mesh_terms: D020341:Absorbable Implants; D001672:Biocompatible Materials / Q000737:chemistry*; D001895:Boron / Q000737:chemistry*; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010710:Phosphates; D012995:Solubility
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D010710:Phosphates; C009753:fiberglass; C000499:ferric oxide; D001895:Boron
keywords: Bioresorbable composites; Cytocompatibility; Dissolution properties; Mechanical properties; Phosphate glass fibres