title: Extraction, purification and anti-fatigue activity of γ-aminobutyric acid from mulberry (Morus alba L.) leaves.
abstract: Mulberry (Morus alba L.) is a tree species of Moraceae widely distributed in Southern China. In the present study, the white crystal of γ-aminobutyric acid (GABA) was purified from mulberry leaves, and its bioactivity was also investigated. The main results were as follows: first, the crude GABA was extracted from mulberry leaves by using biochemical methods. Then, the crude was purified by chromatography over an S-8 macroporous resin, Sephadex G-10, and 732 cation exchange resin to yield a white crystal. Lavage administration and exposure of GABA to male NIH mice showed no adverse effects on their growth and development. In an endurance capacity test, the average loaded-swimming time of medium dose was 111.60% longer than the control (P < 0.01). Further investigations showed that relative to that of model control, the respective blood lactate (BL) concentrations of low- and medium-dose were 28.52% and 28.81% lower (P < 0.05), whereas the levels of blood urea nitrogen (BUN) were 36.83% and 40.54% lower (P < 0.05), and that of liver glycogen (LG) levels were 12.81% and 17.22% lower (P < 0.05). The results indicated that GABA has an advantage over taurine of anti-fatigue effect. These findings were indicative of the anti-fatigue activity of GABA.
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.|Postdoctoral Research Station, China Academy of Chinese Medicine Science, Beijing 100700, China.
authors.forename: Hengwen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Chemistry, Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Xuanhui
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150036, Heilongjiang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: He
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.
authors.forename: Benjun
authors.initials: B
authors.lastname: Wei
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medicine Science, Beijing 100053, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D005221:Fatigue / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control*; D006003:Glycogen / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D031625:Morus / Q000737:chemistry*; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D013654:Taurine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D013654:Taurine; D019344:Lactic Acid; D005680:gamma-Aminobutyric Acid; D006003:Glycogen