title: Antioxidant Potential and In Situ Analysis of Major and Trace Element Determination of Ood-saleeb, a Known Unani Herbal Medicine by ICP-MS.
abstract: The intention of the present research work was to investigate the antioxidant activity and trace element analysis of Ood-saleeb, a known herbal medicine. Preliminary screening of phytochemicals showed that the extract of Ood-saleeb had flavonoids and phenolics. The significant activities in all antioxidant assays were observed in the extract of Ood-saleeb in comparison with the standard antioxidant with respect to dose of Ood-saleeb. Incredible activities to scavenge reactive oxygen species were also observed by the extract of Ood-saleeb. The IC50 values of all factors were determined using ascorbic acid as a standard. The inductive coupled plasma-mass spectroscopy (ICP-MS) was employed for the estimation of trace elements in Ood-saleeb extract. The concentrations of up to 18 elements were detected successfully. Silicon was found in high concentration (85.3 μg/g) while lithium was in low concentration (3 ng/g). The trace elements in the sample were found at different percentage levels which play a key role in the treatment of diseases.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Raish
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Ajaz
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Khalid M
authors.initials: KM
authors.lastname: Alkharfy
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Fahad I
authors.initials: FI
authors.lastname: Al-Jenoobi
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Al-Mohizea
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Kazi
authors.initials: K
authors.lastname: Mohsin
authors.affiliation: Research Centre, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Syed Rizwan
authors.initials: SR
authors.lastname: Ahamad
authors.affiliation: Quality Assurance Unit, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Naushad
authors.initials: N
authors.lastname: Ali
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia. faiyazs@fastmail.fm.|Center of Excellence in Biotechnology Research (CEBR), King Saud University, Riyadh, 11451, Saudi Arabia. faiyazs@fastmail.fm.
authors.forename: Faiyaz
authors.initials: F
authors.lastname: Shakeel
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis; D029001:Herbal Medicine; D013058:Mass Spectrometry; D029596:Paeonia / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014131:Trace Elements / Q000032:analysis*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D017382:Reactive Oxygen Species; D014131:Trace Elements
keywords: Antioxidant; Ascorbic acid; ICP-MS; Ood-saleeb; Trace elements