title: Tobacco smoking and oxidative stress to DNA: a meta-analysis of studies using chromatographic and immunological methods.
abstract: Oxidative stress to DNA from smoking was investigated in one randomized smoking cessation study and in 36 cohort studies from excretion of urinary 8-oxo-7-hydrodeoxyguanosine (8-oxodG). Meta-analysis of the 36 cohort studies showed smoking associated with a 15.7% (95% CL 11.0:20.3, p < 0.0001) increased oxidative stress to DNA, in agreement with the reduction of oxidative stress to DNA found in the smoking cessation study. Meta-analysis of the 22 studies that used chromatography methodology on 1709 persons showed a significant 29.3% increase in smokers (95% CL 17.3;41.3), but meta-analysis of 14 studies on 3668 persons using ELISA methodology showed a non-significant effect of 8.7% [95% CL -1.2;18.6]. Tobacco smoke induces oxidative damage to DNA; however, this is not detected with ELISA methodology. Currently, the use of existing ELISA methodology to measure urinary excretion of 8-oxo-7-hydrodeoxyguanosine cannot be recommended.
authors.affiliation: a Research Unit, Mental Health Service , Esbjerg , Denmark ;
authors.forename: Pernille K
authors.initials: PK
authors.lastname: Ellegaard
authors.affiliation: b Department of Clinical Pharmacology , Bispebjerg Hospital , Copenhagen N , Denmark ;|c Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark ;|d Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark.
authors.forename: Henrik E
authors.initials: HE
authors.lastname: Poulsen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000652:urine; D002853:Chromatography, Liquid; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018384:Oxidative Stress; D012907:Smoking / Q000652:urine*; D016540:Smoking Cessation
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: DNA oxidation; oxidative stress; tobacco