title: Tomato (Solanum lycopersicum) variety discrimination and hybridization analysis based on the 5S rRNA region.
abstract: The tomato (Solanum lycopersicum) is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylogenetic relationship and hybridization information of tomato varieties is very important for further variety breeding. Thus, in this study, we collected 26 tomato varieties and attempted to distinguish them based on the 5S rRNA region, which is widely used in the determination of phylogenetic relations. Sequence analysis of the 5S rRNA region suggested that a large number of nucleotide variations exist among tomato varieties. These variable nucleotide sites were also informative regarding hybridization. Chromas sequencing of Yellow Mountain View and Seuwiteuking varieties indicated three and one variable nucleotide sites in the non-transcribed spacer (NTS) of the 5S rRNA region showing hybridization, respectively. Based on a phylogenetic tree constructed using the 5S rRNA sequences, we observed that 16 tomato varieties were divided into three groups at 95% similarity. Rubiking and Sseommeoking, Lang Selection Procedure and Seuwiteuking, and Acorn Gold and Yellow Mountain View exhibited very high identity with their partners. This work will aid variety authentication and provides a basis for further tomato variety breeding.
authors.affiliation: School of Life Sciences, Ludong University , Yantai , Shandong , P. R. China.
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Sun
authors.affiliation: Department of Horticulture, Kangwon National University , Chuncheon , Korea.
authors.forename: Ho-Min
authors.initials: HM
authors.lastname: Kang
authors.affiliation: Department of Plant & Food Science, Sangmyung University , Cheonan , Korea.
authors.forename: Young-Sik
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Agricultural and Life Science Research Institute, Kangwon National University , Chuncheon , Korea.
authors.forename: Jun-Pill
authors.initials: JP
authors.lastname: Baek
authors.affiliation: School of Life Sciences, Ludong University , Yantai , Shandong , P. R. China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Zheng
authors.affiliation: School of Life Sciences, Ludong University , Yantai , Shandong , P. R. China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Xiang
authors.affiliation: Department of Bio-Health Technology, Kangwon National University, Chuncheon, Korea; Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
authors.forename: Soon-Kwan
authors.initials: SK
authors.lastname: Hong
keywords: 5S rRNA region; genetic diversity; phylogenetic relationship; tomato variety; variety discrimination