title: Cultivation of Spirulina maxima in medium supplemented with sugarcane vinasse.
abstract: The feasibility of sugarcane vinasse as supplement in growth medium of Spirulina maxima was investigated. The cell was cultivated under autotrophic (no vinasse, 70 μmol photons m(-2) s(-1)), heterotrophic (no light, culture medium supplemented with vinasse at 0.1% v/v and 1.0% v/v) and mixotrophic conditions (70 μmol photons m(-2) s(-1), vinasse at 0.1% v/v and 1.0% v/v). These preliminary results suggested a cyclic two-stage cultivation - CTSC, with autotrophic condition during light phase of the photoperiod (12 h, 70-200 μmol photons m(-2) s(-1)) and heterotrophic condition during dark phase (12h, 3.0% v/v vinasse). The adopted CTSC strategy consisted in three cycles with 75% withdrawal of suspension and reposition of medium containing 3.0% v/v vinasse, separated by autotrophic rest periods of few days between cycles. Results show an increase of biomass concentration between 0.495 g L(-1) and 0.609 g L(-1) at the 7th day of each cycle and high protein content (between 74.3% and 77.3% w/w).
authors.affiliation: Escola de Química, Federal University of Rio de Janeiro, Av. Horácio Macedo, 2030, Centro de Tecnologia, Bl. E, Sala 201, Rio de Janeiro, RJ 21941-909, Brazil. Electronic address: raquel.c.rezende@gmail.com.
authors.forename: Raquel Rezende
authors.initials: RR
authors.lastname: Dos Santos
authors.affiliation: Escola de Química, Federal University of Rio de Janeiro, Av. Horácio Macedo, 2030, Centro de Tecnologia, Bl. E, Sala 201, Rio de Janeiro, RJ 21941-909, Brazil.
authors.forename: Ofélia de Queiroz Fernandes
authors.initials: OQF
authors.lastname: Araújo
authors.affiliation: Escola de Química, Federal University of Rio de Janeiro, Av. Horácio Macedo, 2030, Centro de Tecnologia, Bl. E, Sala 201, Rio de Janeiro, RJ 21941-909, Brazil.
authors.forename: José Luiz
authors.initials: JL
authors.lastname: de Medeiros
authors.affiliation: Instituto de Química, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos 149, Centro de Tecnologia, Bl. A Sala 532, Rio de Janeiro, RJ 21941-909, Brazil.
authors.forename: Ricardo Moreira
authors.initials: RM
authors.lastname: Chaloub
mesh_terms: D018533:Biomass; D019149:Bioreactors; D003470:Culture Media; D007220:Industrial Waste; D008027:Light; D058086:Microalgae / Q000254:growth & development; D031786:Saccharum / Q000737:chemistry*; D053545:Spirulina / Q000254:growth & development*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D003470:Culture Media; D007220:Industrial Waste
keywords: Biochemical composition; Cyclic two-stage cultivation; Microalgae cultivation; Spirulina maxima; Sugarcane vinasse