title: Relationship between hOGG1 Ser326Cys gene polymorphism and coronary artery lesions in patients with diabetes mellitus.
abstract: To study the relationship between human 8-oxoguanine glycosylase (hOGG1) Ser326Cys gene polymorphism and coronary artery lesions in patients with diabetes mellitus, we analyzed 323 patients with diabetic mellitus, who underwent coronary angiography. Using PCR-RFLP, these patients were grouped into three genotypes: Cys/Cys (n=85), Ser/Ser (n=121), and Ser/Cys (n=117). Several clinical data, including history of diseases and biochemical indices were recorded. hOGG1 mRNA expression and 8-hydroxy deoxyguanosine (8-OHdG) were measured by RT-PCR and ELISA, respectively. The quantities and severity of coronary artery with lesions were analyzed from coronary angiography. The Gensini and SYNTAX scores were detected by the unitary criteria. The 8-OHdG levels showed statistical difference among the three genotypes (F=21.56, P<0.05). Also, 8-OHdG in Cys/Cys genotype was higher than Ser/Ser and Ser/Cys genotype (q=2.32, q=3.12, P<0.05). In terms of the expression of hOGGl mRNA, the measure of hOGGl/β-actin showed significant difference among the three groups (F=12.56, P<0.05). On comparing two groups, hOGGl/β-actin in Cys/Cys genotype was higher thanSer/Ser and Ser/Cys genotypes (q=2.32, q=3.12, P<0.05). Percentage of 3-vessel lesions was high in Cys/Cys genotype and percentage of 1-vessel lesions was low in Ser/Cys genotype. Gensini and SYNTAX scores and ratio of complex lesions were significantly higher in the Cys/Cys genotype than the other two genotypes (FGensini=47.16, FSYNTAX=55.12; P<0.05). hOGG1 Ser326Cys polymorphism showed correlation with coronary artery lesions in patients with diabetes mellitus, and Cys/Cys genotype may have more impact on the severity of lesions.
authors.affiliation: Graduate Department of Medicine College, Nanchang UniversityNanchang 330031, China; Department of Cardiology, Jiangxi Provincial People's HospitalNanchang 338025, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanchang University Nanchang, China.
authors.forename: Meng-Hong
authors.initials: MH
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanchang University Nanchang, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Qi
authors.affiliation: Cardiovascular Disease Institute of Jiangxi Province Nanchang, China.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Wu
authors.affiliation: Cardiovascular Disease Institute of Jiangxi Province Nanchang, China.
authors.forename: Yu-Zhi
authors.initials: YZ
authors.lastname: Ge
authors.affiliation: Cardiovascular Disease Institute of Jiangxi Province Nanchang, China.
authors.forename: Hua-Tai
authors.initials: HT
authors.lastname: Li
keywords: 8-hydroxy deoxyguanosine; coronary artery lesions; diabetes mellitus; hOGG1; polymorphism