title: Covalent interlocking of glucose oxidase and peroxidase in the voids of paper: enzyme-polymer "spider webs".
abstract: A modular, general method for trapping enzymes within the voids of paper, without chemical activation of cellulose, is reported. Glucose oxidase and peroxidase were crosslinked with poly(acrylic acid) via carbodiimide chemistry, producing 3-dimensional networks interlocked in cellulose fibers. Interlocking prevented enzyme activity loss and enhanced the washability and stability.
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-2060, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Riccardi
authors.forename: D
authors.initials: D
authors.lastname: Mistri
authors.forename: O
authors.initials: O
authors.lastname: Hart
authors.forename: M
authors.initials: M
authors.lastname: Anuganti
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.forename: R M
authors.initials: RM
authors.lastname: Kasi
authors.forename: C V
authors.initials: CV
authors.lastname: Kumar
mesh_terms: D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D010209:Paper; D010544:Peroxidases / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D011108:Polymers; D005949:Glucose Oxidase; D010544:Peroxidases