title: IL-17A up-regulates expression of endothelial tissue factor in liver cirrhosis via the ROS/p38 signal pathway.
abstract: Interleukin-17A (IL-17A), an inflammatory cytokine, is elevated in liver cirrhosis. Inflammation and coagulation dysfunction are closely related. Tissue factor (TF) is a bridge between endothelial activation, blood coagulation and inflammation. The aims of the present study were to evaluate endothelial TF expression in liver cirrhosis and identify the possible underlying role of IL-17A in TF expression. In the present study, we found that TF expression was increased on endothelium of splenic vein from cirrhotic patients and significantly correlated with intima/media ratios of splenic vein and coagulation parameters. Serum levels of IL-17A were significantly higher in cirrhotic patients as compared with normal controls. Cirrhotic serum and IL-17A stimulated TF expression in HUVECs, which was reduced by blockade of IL-17A, p38, and reactive oxygen species (ROS). Taken together, our data show that enhanced expression of endothelial TF, which plays an important role in coagulopathy and splenic vein remodeling in liver cirrhosis, is induced by IL-17A in a ROS dependent manner.
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, Shaanxi, Xian 710004, China.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Pu
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, Shaanxi, Xian 710004, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, Shaanxi, Xian 710004, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, Shaanxi, Xian 710004, China.
authors.forename: Na
authors.initials: N
authors.lastname: Huang
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Shaanxi, Xi'an 710004, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. Electronic address: yangjundr@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, Shaanxi, Xian 710004, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Shaanxi, Xi'an 710004, China. Electronic address: lzf2568@gmail.com.
authors.forename: Zongfang
authors.initials: Z
authors.lastname: Li
mesh_terms: D004730:Endothelium, Vascular / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D013162:Splenic Vein / Q000378:metabolism*; D013925:Thromboplastin / Q000378:metabolism*; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C508717:IL17A protein, human; D020381:Interleukin-17; D017382:Reactive Oxygen Species; D013925:Thromboplastin; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Endothelial cells; Interleukin-17; Liver cirrhosis; Reactive oxygen species; Tissue factor