title: Deep-sequencing of the T-cell receptor repertoire in patients with haplo-cord and matched-donor transplants.
abstract: Haplo-cord transplant has emerged as a feasible and reliable approach for haematopoietic stem cell transplant in patients who are unable to find matched-donor. This approach provides fast myeloid recovery, low incidence of graft vs host disease (GVHD) and favorable graft versus leukemia (GVL) effects. T cell recovery plays an important role in preventing infectious complications; it also mediates the GVHD and the GVL effects. Here, we utilized a novel RNA-based sequencing approach to quantitatively characterize the T cell receptor (TCRs) repertoire in patients underwent haplo-cord transplant in comparison with those underwent matched-donor transplant. Our study shows that higher percentage of cord cells early post transplant were associated with significantly higher TCR diversity. TCR diversity was significantly lower in patients with GVHD and in relapsed patients. A larger cohort study is needed to validate these data and to provide useful information on the specific TCR clones correlated with clinical outcome.
authors.affiliation: a School of Pharmacy, University of Southern California , Los Angeles , CA , USA.
authors.forename: Houda
authors.initials: H
authors.lastname: Alachkar
authors.affiliation: b Section of Hematology/Oncology, Department of Medicine, The University of Chicago , Chicago , IL , USA.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D006086:Graft vs Host Disease / Q000235:genetics; D020544:Graft vs Leukemia Effect / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000628:therapy; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D061349:Unrelated Donors
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: GVHD; GVL; Haplo-cord; T cell receptors; T cell repertoire; chimerism; relapse; stem cell transplant