title: [Screening and identification of a bacterium capable of converting agar to neoagaro oligosaccharides].
abstract: OBJECTIVE
  To screen and identify a bacterium capable of converting agar to neoagaro oligosaccharides.
  
  
  METHODS
  We took samples of porphyra haitanensis and nearby seawater, and then used the medium containing 1 per thousand agar to enrich the target bacteria. The target isolates were obtained by dilution-plate method, of which crude enzymes were further obtained by liquid culture. We adopted DNS method to determine the target bacteria which can convert agar to neoagaro oligosaccharides. The phylogenetics was identified by analyzing 16S rDNA sequence and combining the strain's morphological and bacterial colonial physiological biochemical characteristics.
  
  
  RESULTS
  We isolated a gram-negative bacterial strain HJPHYXJ-1 capable of transforming agar to neoagaro oligosaccharides. Basic Local Alignment Search Tool (BLAST) search of HJPHYXJ-1's 16S rDNA sequence on GenBank suggested that the similarity between this strain and Vibrio natriegens reached 99% . In addition, the morphological and physiological biochemical characteristics of HJPHYXJ-1 also showed highly similarity to Vibrio natriegens. So we identified HJPHYXJ-1 as Vibrio natriegens. The results of HPLC suggested that the metabolite of enzymatic degradation was neoagaro oligosaccharides.
  
  
  CONCLUSIONS
  HJPHYXJ-1 or the new isolate of Vibrio natriegens was capable of converting agar to neoagaro oligosaccharides.
authors.forename: Junping
authors.initials: J
authors.lastname: Han
authors.forename: Yayan
authors.initials: Y
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.forename: Meitian
authors.initials: M
authors.lastname: Xiao
mesh_terms: D000362:Agar / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D014733:Vibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009844:Oligosaccharides; D012336:RNA, Ribosomal, 16S; D000362:Agar