title: Gingko biloba extract (Ginaton) ameliorates dextran sulfate sodium (DSS)-induced acute experimental colitis in mice via reducing IL-6/STAT3 and IL-23/IL-17.
abstract: This study explored the underlying mechanism of Gingko biloba extract (Ginaton) on dextran sulfate sodium (DSS)-induced acute experimental colitis in mice. 40 male C57BL/6 mice were randomly divided into four groups: normal control group, Ginaton group, Ginaton treatment group, and DSS group. After 7 days administration, mice were sacrificed and colons were collected for H-E staining, immunohistochemistry, real-time PCR and Western blot. By observing clinical disease activity and histological damage, we assessed the effect of Ginaton on DSS-induced acute experimental colitis in mice and observed the effect of Ginaton on normal mice. We also explored the specific mechanism of Ginaton on DSS-induced acute experimental colitis in mice through examining the expression of inflammatory related mediators (gp130, STAT3, p-STAT3, ROR-γt) and cytokines (IL-6, IL-17, IL-23). Ginaton-treated DSS mice showed significant improvement over untreated DSS mice. Specifically, Ginaton improved clinical disease activity (DAI score, weight closs, colon shortening, and bloody stool) and histological damage, and reduced the expression of inflammatory-related mediators (p-STAT3, gp130, ROR-γt) and cytokines (IL-6, IL-17, IL-23). In addition, clinical disease activity, histological damage, the expression of inflammatory related mediators (STAT3, p-STAT3, gp130, ROR-t) and cytokines (IL-6, IL-17, IL-23) in mice of Ginaton group were similar to normal control group. In conclusion, Ginaton ameliorates DSS-induced acute experimental colitis in mice by reducing IL-17 production, which is at least partly involved in inhibiting IL-6/STAT3 signaling pathway and IL-23/IL-17 axis. Moreover, Ginaton itself does not cause inflammatory change in normal mice. These results support that Ginaton can be as a potential clinical treatment for ulcerative colitis (UC).
authors.affiliation: Department of Gastroenterology, Shengjing Hospital of China Medical University 39 Huaxiang Road, Tiexi District, Shenyang 110022, Liaoning Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Shengjing Hospital of China Medical University 39 Huaxiang Road, Tiexi District, Shenyang 110022, Liaoning Province, China.
authors.forename: Lian-Jie
authors.initials: LJ
authors.lastname: Lin
authors.affiliation: Department of Gastroenterology, Shengjing Hospital of China Medical University 39 Huaxiang Road, Tiexi District, Shenyang 110022, Liaoning Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of China Medical University 155 Nanjing North Street, Heping District, Shenyang 110001, Liaoning Province, China.
authors.forename: Li-Xuan
authors.initials: LX
authors.lastname: Sang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of China Medical University 155 Nanjing North Street, Heping District, Shenyang 110001, Liaoning Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, Shengjing Hospital of China Medical University 39 Huaxiang Road, Tiexi District, Shenyang 110022, Liaoning Province, China.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zheng
keywords: Ginaton; IL-23/IL-17; IL6/STAT3; acute experimental colitis