title: Enhanced and Differential Capture of Circulating Tumor Cells from Lung Cancer Patients by Microfluidic Assays Using Aptamer Cocktail.
abstract: Collecting circulating tumor cells (CTCs) shed from solid tumor through a minimally invasive approach provides an opportunity to solve a long-standing oncology problem, the real-time monitoring of tumor state and analysis of tumor heterogeneity. However, efficient capture and detection of CTCs with diverse phenotypes is still challenging. In this work, a microfluidic assay is developed using the rationally-designed aptamer cocktails with synergistic effect. Enhanced and differential capture of CTCs for nonsmall cell lung cancer (NSCLC) patients is achieved. It is also demonstrated that the overall consideration of CTC counts obtained by multiple aptamer combinations can provide more comprehensive information in treatment monitoring.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Libo
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Lung Cancer, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, P. R. China.
authors.forename: Chuanhao
authors.initials: C
authors.lastname: Tang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Lung Cancer, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Oncology, Translational Medicine Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, P. R. China.
authors.forename: Haixu
authors.initials: H
authors.lastname: Hu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Molecular and Medical Pharmacology, California NanoSystems Institute, University of California, Los Angeles, CA, 90095, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Fong
authors.affiliation: Laboratory of Oncology, Translational Medicine Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, P. R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Molecular and Medical Pharmacology, California NanoSystems Institute, University of California, Los Angeles, CA, 90095, USA.
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
authors.affiliation: Department of Lung Cancer, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, P. R. China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Gao
authors.affiliation: Laboratory of Oncology, Translational Medicine Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D044085:Microfluidics / Q000379:methods*; D053770:Nanowires; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D015203:Reproducibility of Results; D012825:Silicon / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D012825:Silicon
keywords: aptamers; cancer diagnosis; circulating tumor cells; rare cell enrichment; tumor heterogeneity