title: Ionic liquids-lithium salts pretreatment followed by ultrasound-assisted extraction of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside and vitexin from Phyllostachys edulis leaves.
abstract: An efficient method for the extraction of vitexin, vitexin-4″-O-glucoside, and vitexin-2″-O-rhamnoside from Phyllostachys edulis leaves comprises heat treatment using an ionic liquid-lithium salt mixture (using 1-butyl-3-methylimidazolium bromide as the solvent and lithium chloride as the additive), followed by ultrasound-assisted extraction. To obtain higher extraction yields, the effects of the relevant experimental parameters (including heat treatment temperature and time, relative amounts of 1-butyl-3-methylimidazolium bromide and lithium chloride, power and time of the ultrasound irradiation, and the liquid-solid ratio) are evaluated and response surface methodology is used to optimize the significant factors. The morphologies of the treated and untreated P. edulis leaves are studied by scanning electron microscopy. The improved extraction method proposed provides high extraction yield, good repeatability and precision, and has wide potential applications in the analysis of plant samples.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China; State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, 150040 Harbin, China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Hou
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China; State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, 150040 Harbin, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China; State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, 150040 Harbin, China.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China; State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, 150040 Harbin, China. Electronic address: ylhounefu@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D047310:Apigenin / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D007529:Isoflavones / Q000302:isolation & purification; D008094:Lithium / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014465:Ultrasonics
chemical_list: D005960:Glucosides; D007093:Imidazoles; D052578:Ionic Liquids; D007529:Isoflavones; D010936:Plant Extracts; D012997:Solvents; C477953:isovitexin-4'-O-glucoside; C021329:vitexin rhamnoside; C502841:1-butyl-3-methylimidazolium chloride; D047310:Apigenin; D008094:Lithium; C032731:vitexin
keywords: Ionic liquid; Lithium salt; Phyllostachys edulis; Ultrasound-assisted extraction; Vitexin; Vitexin-2″-O-rhamnoside-; Vitexin-4″-O-glucoside