title: Protective Effect of Ethanol Extracts of the Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), on the Experimental Middle Cerebral Artery Occlusion/Reperfusion (MCAO/R) Model.
abstract: In this study, we investigated the effects of ethanol extracts of Ophiocordyceps sinensis (EEOS) on neuroprotective efficacy in a rat model of focal cerebral ischemia/reperfusion (IR). The effects of EEOS on mortality rate, neurobehavior, grip strength, polymorphonuclear (PMN) cells, interleukin (IL)-1β, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, intracellular adhesion molecule 1 (ICAM-1), and cyclooxygenase-2 (COX-2) were determined by enzyme-linked immunosorbent assay and immunohistochemistry. The cerebral infarction was examined through tetrazolium chloride staining. EEOS significantly inhibited IR-induced brain production of IL-1β, TNF-α, iNOS, ICAM-1, and COX-2. Moreover, EEOS suppressed infiltration of PMN cells. EEOS caused a significant reduction in the infarct size compared with the middle cerebral artery occlusion group. The study demonstrates the neuroprotective potential of EEOS inhibition of IR through anti-inflammatory activity in a rat model of IR.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Kong
authors.affiliation: Department of Dermatology, The First Hospital of Chinese People's Liberation Army, Gansu, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Shuofeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xing
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Guan
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People's Republic of China.
authors.forename: Chunchao
authors.initials: C
authors.lastname: Han
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001203:Ascomycota / Q000737:chemistry*; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000517:prevention & control* / Q000628:therapy; D008297:Male; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018696:Neuroprotective Agents