title: Large molecular systems landscape uncovers T cell trapping in human skin cancer.
abstract: Immune surveillance of tumour cells is an important function of CD8 T lymphocytes, which has failed in cancer for reasons still unknown in many respect but mainly related to cellular processes in the tumour microenvironment. Applying imaging cycler microscopy to analyse the immune contexture in a human skin cancer we could identify and map 7,000 distinct cell surface-associated multi-protein assemblies. The resulting combinatorial geometry-based high-functional resolution led to discovery of a mechanism of T cell trapping in the epidermis, which involves SPIKE, a network of suprabasal keratinocyte projections piercing and interconnecting CD8 T cells. It appears initiated by clusters of infrabasal T and dendritic cells connected via cell projections across a fractured basal lamina to suprabasal keratinocytes and T lymphocytes.
authors.affiliation: Molecular Pattern Recognition Research Group, Medical Faculty, OvG University Magdeburg, Leipziger Straße 44, D-39120 Magdeburg, Germany.
authors.forename: Reyk
authors.initials: R
authors.lastname: Hillert
authors.affiliation: Molecular Pattern Recognition Research Group, Medical Faculty, OvG University Magdeburg, Leipziger Straße 44, D-39120 Magdeburg, Germany.|Department for Structural Cell Biology, Center for Biological Systems Analysis (ZBSA), Albert-Ludwigs-University,Freiburg, Habsburger Straße 49, D-79104 Freiburg,Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Gieseler
authors.affiliation: Molecular Pattern Recognition Research Group, Medical Faculty, OvG University Magdeburg, Leipziger Straße 44, D-39120 Magdeburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Krusche
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Humme
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Röwert-Huber
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Sterry
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Walden
authors.affiliation: Molecular Pattern Recognition Research Group, Medical Faculty, OvG University Magdeburg, Leipziger Straße 44, D-39120 Magdeburg, Germany.|Human Toponome Project (HUTO), Margaretenstraße 20, D-81373 Munich, Germany.
authors.forename: Walter
authors.initials: W
authors.lastname: Schubert
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008954:Models, Biological; D012878:Skin Neoplasms / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D059016:Tumor Microenvironment / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers