title: Perfluorocarbon attenuates inflammatory cytokines, oxidative stress and histopathologic changes in paraquat-induced acute lung injury in rats.
abstract: The effects of perfluorocarbon (PFC) on paraquat (PQ) induced acute lung injury (ALI) was evaluated among rats. Twenty four Wistar rats were divided into 4 groups: control group injected by saline physiologic 0.9%, PFC group injected by Perfluorocarbon, PQ group injected by PQ and PQ+PFC group injected by PFC one hour after receiving paraquat. Bronchoalveular fluid content, inflammatory cytokines, oxidative and histopathologic changes were measured after 72 h. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and transforming growth factor-β1(TGF-β1) in the PQ group were increased compared to either control or PFC groups, but their levels decreased in PQ+PFC group significantly (p<0.05). Also, histopathologic evaluation revealed an increase in malondialdehyde (MDA) and hydroxyproline (HP) in the PQ group but a decrease in PQ+PFC group significantly (p<0.01). PFC emulsion by its anti-inflammatory, anti-oxidative and anti-fibrotic properties can reduce the inflammatory and fibrotic alterations, pulmonary oedema, and pulmonary histopathologic changes created by PQ.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khalighi
authors.affiliation: Student Researches Committee, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Rahmani
authors.affiliation: Department of Physiology, Faculty of Veterinary, Ilam University, Ilam, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Cheraghi
authors.affiliation: Department of Pathology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Mohammad Reza Hafezi
authors.initials: MR
authors.lastname: Ahmadi
authors.affiliation: Department of Cardiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Koroush
authors.initials: K
authors.lastname: Soleimannejad
authors.affiliation: Department of Pathology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Ruhangiz
authors.initials: R
authors.lastname: Asadollahi
authors.affiliation: Department of Epidemiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran. Electronic address: Masoud_1241@yahoo.co.uk.
authors.forename: Khairollah
authors.initials: K
authors.lastname: Asadollahi
mesh_terms: D055371:Acute Lung Injury; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D005466:Fluorocarbons / Q000494:pharmacology*; D015850:Interleukin-6; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005466:Fluorocarbons; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D010269:Paraquat
keywords: Histopathology; Inflammation; Lung; Malondealdehyde; Paraquat; Perfluorocarbon