title: Adsorption and Unfolding of a Single Protein Triggers Nanoparticle Aggregation.
abstract: The response of living systems to nanoparticles is thought to depend on the protein corona, which forms shortly after exposure to physiological fluids and which is linked to a wide array of pathophysiologies. A mechanistic understanding of the dynamic interaction between proteins and nanoparticles and thus the biological fate of nanoparticles and associated proteins is, however, often missing mainly due to the inadequacies in current ensemble experimental approaches. Through the application of a variety of single molecule and single particle spectroscopic techniques in combination with ensemble level characterization tools, we identified different interaction pathways between gold nanorods and bovine serum albumin depending on the protein concentration. Overall, we found that local changes in protein concentration influence everything from cancer cell uptake to nanoparticle stability and even protein secondary structure. We envision that our findings and methods will lead to strategies to control the associated pathophysiology of nanoparticle exposure in vivo.
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Sergio
authors.initials: S
authors.lastname: Dominguez-Medina
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Lydia
authors.initials: L
authors.lastname: Kisley
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Tauzin
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Anneli
authors.initials: A
authors.lastname: Hoggard
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Shuang
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: A Swarnapali D S
authors.initials: AS
authors.lastname: Indrasekara
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Sishan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Lin-Yung
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Derry
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.
authors.forename: Anton
authors.initials: A
authors.lastname: Liopo
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.|Department of Materials Science and NanoEngineering, Rice University , Houston, Texas 77251, United States.
authors.forename: Eugene R
authors.initials: ER
authors.lastname: Zubarev
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.|Department of Electrical and Computer Engineering, Rice University , Houston, Texas 77251, United States.
authors.forename: Christy F
authors.initials: CF
authors.lastname: Landes
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77251, United States.|Department of Electrical and Computer Engineering, Rice University , Houston, Texas 77251, United States.
authors.forename: Stephan
authors.initials: S
authors.lastname: Link
mesh_terms: D000327:Adsorption; D006046:Gold / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D000066970:Protein Corona / Q000737:chemistry* / Q000378:metabolism*; D058767:Protein Unfolding
chemical_list: D000066970:Protein Corona; D006046:Gold
keywords: correlation spectroscopy; nanorods; protein corona; superlocalization microscopy; surface plasmon