title: The linker between LOV2-Jα and STK plays an essential role in the kinase activation by blue light in Arabidopsis phototropin1, a plant blue light receptor.
abstract: Phototropin (phot), a blue light receptor in plants, is composed of several domains: LOV1, LOV2, and a serine/threonine kinase (STK). LOV2 is the main regulator of light activation of STK. However, the detailed mechanism remains unclear. In this report, we focused on the linker region between LOV2 and STK excluding the Jα-helix. Spectroscopy and a kinase assay for the substituents in the linker region of Arabidopsis phot1 LOV2-STK indicated that the linker is involved in the activation of STK. A putative module in the middle of the linker would be critical for intramolecular signaling and/or regulation of STK.
authors.affiliation: Department of Biological Science, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kashojiya
authors.affiliation: Department of Biological Science, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Shizue
authors.initials: S
authors.lastname: Yoshihara
authors.affiliation: Department of Biological Science, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Okajima
authors.affiliation: Department of Biological Science, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Tokutomi
mesh_terms: D019943:Amino Acid Substitution; D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation / Q000528:radiation effects; D017353:Gene Deletion; D006860:Hydrogen Bonding / Q000528:radiation effects; D008027:Light; D026161:MADS Domain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000528:radiation effects; D056949:Phototropins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D015202:Protein Engineering; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C450148:LKP2 protein, Arabidopsis; C527342:LOV1 protein, Arabidopsis; D026161:MADS Domain Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D056949:Phototropins; D020033:Protein Isoforms; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C476012:SEEDSTICK protein, Arabidopsis; C103646:NPH1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: blue light; phototropin; serine/threonine kinase