title: No selection of CXCR4-using variants in cell reservoirs of dual-mixed HIV-infected patients on suppressive maraviroc therapy.
abstract: We used ultradeep sequencing to investigate the evolution of the frequency of CXCR4-using viruses in the peripheral blood mononuclear cells of 22 patients infected with both CCR5 and CXCR4-using viruses treated with the CCR5 antagonist maraviroc for 24 weeks and a stable antiviral therapy. The mean CXCR4-using virus frequency in peripheral blood mononuclear cells was 59% before maraviroc intensification and 52% after 24 weeks of effective treatment, indicating no selection by maraviroc.
authors.affiliation: aINSERM,UMR1043 bUniversité Toulouse III Paul-Sabatier, Faculté de Médecine Toulouse-Purpan cCHU de Toulouse, Hôpital Purpan, Laboratoire de Virologie dINSERM, UMR1027 eUniversité de Toulouse III fCHU Toulouse, COREVIH gCHU de Toulouse, Hôpital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Raymond
authors.forename: Florence
authors.initials: F
authors.lastname: Nicot
authors.forename: Romain
authors.initials: R
authors.lastname: Carcenac
authors.forename: Nicolas
authors.initials: N
authors.lastname: Jeanne
authors.forename: Michelle
authors.initials: M
authors.lastname: Cazabat
authors.forename: Mary
authors.initials: M
authors.lastname: Requena
authors.forename: Lise
authors.initials: L
authors.lastname: Cuzin
authors.forename: Pierre
authors.initials: P
authors.lastname: Delobel
authors.forename: Jacques
authors.initials: J
authors.lastname: Izopet
mesh_terms: D019380:Anti-HIV Agents / Q000008:administration & dosage*; D003510:Cyclohexanes / Q000008:administration & dosage*; D005838:Genotype; D006678:HIV / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D000077592:Maraviroc; D012641:Selection, Genetic; D014230:Triazoles / Q000008:administration & dosage*; D056189:Viral Tropism
chemical_list: D019380:Anti-HIV Agents; D003510:Cyclohexanes; D014230:Triazoles; D000077592:Maraviroc