title: Potential Skin Regeneration Activity and Chemical Composition of Absolute from Pueraria thunbergiana Flower.
abstract: The flower of Pueraria thunbergiana BENTH (PTBF) contains isoflavonoids and essential oil components. It has many biological and pharmacological activities, including anti-diabetes, anti-oxidant, and weight loss. However, its effect on skin regeneration remains unknown. In the present study, we isolated the absolute from PTBF through solvent extraction and determined the role of the absolute on skin regeneration-associated responses in human epidermal-keratinocytes (HaCats). The PTBF absolute, which contained 10 compounds, stimulated migration and proliferation and increased the phosphorylation of serine/threonine-specific protein kinase and extracellular signal-regulated kinasel/2 in HaCats. It induced type I and IV collagen synthesis in HaCats. In addition, treatment with PTBF absolute resulted in increased sprout outgrowth in HaCats. These findings suggest that PTBF absolute may participate in skin regeneration, probably through promotion of migration, proliferation, and collagen synthesis.
authors.forename: Do-Yoon
authors.initials: DY
authors.lastname: Kim
authors.forename: Kyung-Jong
authors.initials: KJ
authors.lastname: Won
authors.forename: Dae-Il
authors.initials: DI
authors.lastname: Hwang
authors.forename: Seok Won
authors.initials: SW
authors.lastname: Yoon
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Joo-Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Myeong Sik
authors.initials: MS
authors.lastname: Yoon
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Lee
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024141:Collagen Type IV / Q000378:metabolism; D035264:Flowers / Q000737:chemistry; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry*; D012038:Regeneration / Q000187:drug effects; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology*; D015595:Skin Aging / Q000187:drug effects
chemical_list: D024141:Collagen Type IV; D010936:Plant Extracts