title: Whole body radioprotective effect of phenolic extracts from the fruits of Malus baccata (Linn.) Borkh.
abstract: This study was designed to evaluate the radioprotective effect of phenolics extracted from the fruits of Malus baccata (Linn.) Borkh. (MBP-3b) against damage induced by (60)Co γ-irradiation in vivo. MBP-3b could significantly improve the activity of endogenous antioxidant enzymes and the T-AOC, as well as reduce the MDA level in the liver and kidneys of irradiated mice. In addition, pretreatment with MBP-3b at a dose of 150 mg per kg bw could significantly enhance immunomodulation activity by promoting the proliferation of spenocytes and monocyte phagocytosis. The administration of MBP-3b prevented the decline induced by radiation of haematological parameters (WBC, RBC, PLT and HGB). Furthermore, MBP-3b could protect spenocytes from radiation-induced damage by inhibiting cell apoptosis. The results indicated that MBP-3b possesses strong whole body radioprotective and immunomodulatory activities. The main constituents of MBP-3b were tentatively identified as delphinidin-3,5-diglucoside, cyanidin-3-glucoside, chlorogenic acid, proanthocyanidin C1, quercetin-3-galactoside, quercetin-3-glucoside, quercetin-3-xyloside/arabinoside, phloretin-2-xyloseglucoside, quercetin-3-rhamnoside and phlorizin. MBP-3b could be used as a probable radioprotector against gamma radiation induced oxidative damage.
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. wl@ysu.edu.cn.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. wl@ysu.edu.cn.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China. wangzhenyu103528@126.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002726:Chlorogenic Acid / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D005720:Gamma Rays / Q000009:adverse effects; D005960:Glucosides / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D027845:Malus / Q000737:chemistry*; D051379:Mice; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D044945:Proanthocyanidins / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; D011837:Radiation-Protective Agents; C462279:cyanidin-3-O-beta-glucopyranoside; C016527:isoquercitrin; C013221:proanthocyanidin; D002726:Chlorogenic Acid; D008315:Malondialdehyde; D011794:Quercetin; C017185:delphinidin