title: Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp. T241.
abstract: Serratia sp. T241, a newly isolated xylose-utilizing strain, produced three 2,3-butanediol (2,3-BD) stereoisomers. In this study, three 2,3-butanediol dehydrogenases (BDH1-3) and one glycerol dehydrogenase (GDH) involved in 2,3-BD isomers formation by Serratia sp. T241 were identified. In vitro conversion showed BDH1 and BDH2 could catalyzed (3S)-acetoin and (3R)-acetoin into (2S,3S)-2,3-BD and meso-2,3-BD, while BDH3 and GDH exhibited the activities from (3S)-acetoin and (3R)-acetoin to meso-2,3-BD and (2R,3R)-2,3-BD. Four encoding genes were assembled into E. coli with budA (acetolactate decarboxylase) and budB (acetolactate synthase), responsible for converting pyruvate into acetoin. E. coli expressing budAB-bdh1/2 produced meso-2,3-BD and (2S,3S)-2,3-BD. Correspondingly, (2R,3R)-2,3-BD and meso-2,3-BD were obtained by E. coli expressing budAB-bdh3/gdh. These results suggested four enzymes might contribute to 2,3-BD isomers formation. Mutants of four genes were developed in Serratia sp. T241. Δbdh1 led to reduced concentration of meso-2,3-BD and (2S,3S)-2,3-BD by 97.7% and 87.9%. (2R,3R)-2,3-BD with a loss of 73.3% was produced by Δbdh3. Enzyme activity assays showed the decrease of 98.4% and 22.4% by Δbdh1 and Δbdh3 compared with the wild strain. It suggested BDH1 and BDH3 played important roles in 2,3-BD formation, BDH2 and GDH have small effects on 2,3-BD production by Serratia sp. T241.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Liaoyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Zewang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Jiebo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Quanming
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Kaihui
authors.initials: K
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Science, Fujian Agriculture and Forestry University, FuZhou, Fujian province, 350002, PR China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, PR China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Shen
mesh_terms: D002072:Butylene Glycols / Q000737:chemistry* / Q000378:metabolism*; D002384:Catalysis; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D023061:Gene Order; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009876:Operon; D010957:Plasmids / Q000235:genetics; D017384:Sequence Deletion; D012705:Serratia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013237:Stereoisomerism; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D013401:Sugar Alcohol Dehydrogenases; C095946:galactitol 2-dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase