title: The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α.
abstract: Although androgen therapy resistance and poor clinical outcomes are seen in most canine prostate cancer cases, there are only a few tools for analysing canine prostate cancer by using a cell biological approach. Therefore, to evaluate androgen-independent neoplastic cell growth, a new canine prostate cancer cell line (CHP-1) was established in this study. CHP-1 over-expressed the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is over-expressed in human androgen-independent prostate cancer. The CHP-1 xenograft also showed SGTA over-expression. Although CHP-1 shows poor androgen receptor (AR) signalling upon dihydrotestosterone stimulation, forced expression of AR enabled evaluation of AR signalling. Taken together, these results suggest that CHP-1 will be a useful model for investigating the pathogenesis of androgen-dependent and androgen-independent canine prostate cancer.
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Azakami
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Nakahira
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Michishita
authors.affiliation: Department of Veterinary Clinical Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Onozawa
authors.affiliation: Department of Veterinary Clinical Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Bonkobara
authors.affiliation: Department of Reproduction, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishioka
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sako
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ochiai
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Omi
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D005973:Glutamine; D008297:Male; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D000074436:Tetratricopeptide Repeat
chemical_list: D002352:Carrier Proteins; D005973:Glutamine
keywords: androgen receptor signalling; canine; prostate cancer; small glutamine-rich tetratricopeptide repeat-containing protein α