title: Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
abstract: This study presents a novel signal amplification method for high-sensitive electrochemical immunosensing. Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody. The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles. The remarkable conductivity of AuNPs provides a feasible pathway for electron transfer. The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml. Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IR, Iran.
authors.forename: Hanieh
authors.initials: H
authors.lastname: Shirazi
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IR, Iran.
authors.forename: Anita
authors.initials: A
authors.lastname: Ahmadi
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IR, Iran.
authors.forename: Maedeh
authors.initials: M
authors.lastname: Darzianiazizi
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IR, Iran.
authors.forename: Susan
authors.initials: S
authors.lastname: Kashanian
authors.affiliation: Department of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience and Nanotechnology Research Center (NNRC), Faculty of Science, Razi University, Kermanshah, IR, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IR, Iran. omidfar@tums.ac.ir.
authors.forename: Kobra
authors.initials: K
authors.lastname: Omidfar
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D005290:Ferric Compounds; C118071:N-trimethyl chitosan chloride; D012709:Serum Albumin; C000499:ferric oxide; D006046:Gold; D048271:Chitosan