title: Controlled association and delivery of nanoparticles from jet-sprayed hybrid microfibrillar matrices.
abstract: To develop bioactive scaffolds of targeted properties for tissue repair or biomedical applications, hybrid microfiber-nanoparticle (MF-NP) matrices capable of controlled nanoparticle (NP) delivery were prepared through two novel approaches. In a first strategy, the suppleness of the jet-spraying method to produce polymer microfibers (MF) was used to deposit poly(d,l-lactide) (PLA) NP on poly(lactic-co-glycolic acid) (PLGA) MF by direct co-projection. The second approach relied on the post-incubation of PLA NP aqueous dispersion with MF preliminarily prepared by jet-spraying. NP coverage density onto MF and NP release was assessed by scanning electron microscopy and fluorescence measurements using coumarin-6 loaded NP. The first process was shown to allow high coverage density of NP onto MF (300 μg/mg MF) and strong association, with no NP release observed over time. In the second approach, direct incubation of PLA NP with PLA MF led to lower NP coverage density (40 μg/mg MF) with very fast release of NP from MF. The pre-coating of MF with poly-l-lysine (PLL) or the one of NP with lysozyme as a model protein drug afforded a higher coverage density and stronger association, coupled with a more sustained release of NP from MF over time. These results show the possibility to control the immobilization density and release of NP through appropriate preparation process and surface modification, and are of prime interest for the development of complex scaffolds with orchestrated bioactivity.
authors.affiliation: CNRS, University Lyon 1, UMR 5305, Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, 7 Passage du Vercors, 69367 Lyon Cedex 07, France.
authors.forename: Nermin
authors.initials: N
authors.lastname: Keloglu
authors.affiliation: CNRS, University Lyon 1, UMR 5305, Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, 7 Passage du Vercors, 69367 Lyon Cedex 07, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Verrier
authors.affiliation: Aix-Marseille Université, CNRS, UMR 7273, Institut de Chimie Radicalaire, Avenue Escadrille Normandie-Niemen, 13397 Marseille Cedex 20, France. Electronic address: Thomas.trimaille@univ-amu.fr.
authors.forename: Thomas
authors.initials: T
authors.lastname: Trimaille
authors.affiliation: CNRS, University Lyon 1, UMR 5305, Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, 7 Passage du Vercors, 69367 Lyon Cedex 07, France. Electronic address: jerome.sohier@ibcp.fr.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Sohier
mesh_terms: D000327:Adsorption; D003374:Coumarins / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods; D065546:Drug Liberation; D004591:Electrophoresis, Polyacrylamide Gel; D019344:Lactic Acid / Q000737:chemistry*; D020894:Microfibrils / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties; D013844:Thiazoles / Q000737:chemistry
chemical_list: D003374:Coumarins; D003692:Delayed-Action Preparations; D011091:Polyesters; D013844:Thiazoles; C517282:coumarin 6; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D009113:Muramidase
keywords: Controlled delivery; Microfibers; Nanoparticles; PLA; Scaffolds