title: Macrophage Recruitment Contributes to Regeneration of Mechanosensory Hair Cells in the Zebrafish Lateral Line.
abstract: In vertebrates, damage to mechanosensory hair cells elicits an inflammatory response, including rapid recruitment of macrophages and neutrophils. While hair cells in amniotes usually become permanently lost, they readily regenerate in lower vertebrates such as fish. Damage to hair cells of the fish lateral line is followed by inflammation and rapid regeneration; however the role of immune cells in this process remains unknown. Here, we show that recruited macrophages are required for normal regeneration of lateral line hair cells after copper damage. We found that genetic ablation or local ablation using clodronate liposomes of macrophages recruited to the site of injury, significantly delays hair cell regeneration. Neutrophils, on the other hand, are not needed for this process. We anticipate our results to be a starting point for a more detailed description of extrinsic signals important for regeneration of mechanosensory cells in vertebrates. J. Cell. Biochem. 117: 1880-1889, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Simón A
authors.initials: SA
authors.lastname: Carrillo
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.|Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Anguita-Salinas
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Peña
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Morales
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Salomé
authors.initials: S
authors.lastname: Muñoz-Sánchez
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Carlos
authors.initials: C
authors.lastname: Muñoz-Montecinos
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Susana
authors.initials: S
authors.lastname: Paredes-Zúñiga
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Karina
authors.initials: K
authors.lastname: Tapia
authors.affiliation: FONDAP Center for Genome Regulation. Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Miguel L
authors.initials: ML
authors.lastname: Allende
mesh_terms: D000825:Animal Structures / Q000502:physiology*; D000818:Animals; D003300:Copper / Q000633:toxicity*; D008264:Macrophages / Q000276:immunology*; D040542:Mechanotransduction, Cellular / Q000276:immunology*; D009475:Neurons, Afferent / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D012038:Regeneration / Q000276:immunology*; D015027:Zebrafish / Q000276:immunology*
chemical_list: D003300:Copper
keywords: CLODRONATE; INFLAMMATION; MACROPHAGE; REGENERATION; RESOLUTION; ZEBRAFISH