title: Synthesis of pH-responsive β-CD-based star polymer and impact of its self-assembly behavior on pectinase activity.
abstract: A novel type of pH-responsive star polymer based on β-cyclodextrin (β-CD) was synthesized and further covalently conjugated with enzyme. The impact of its self-assembly behavior on enzyme activity was investigated. In our design, azide containing the polymer (N3)7-β-CD-(PtBA)14was synthesized via atom transfer radical polymerization of tert-butyl acrylate using (N3)7-β-CD-(Br)14as the multifunctional initiator. The final product (N3)7-β-CD-(PAA)14was obtained via hydrolysis and covalently conjugating pectinase onto pH-responsive polyacrylic acid (PAA) arms. PAA can change its conformation with the self-assembly by altered pH, leading its nanostructure into micellar nanoparticles in aqueous solution and further affecting the activity of immobilized pectinase. The results were proved by fluorescence spectroscopy and dynamic light scattering. This system proves that the activity of immobilized enzyme can be tailored predictably, and this pH-responsive polymer holds great potential for controllable delivery of enzymes.
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hu
authors.affiliation: Basic Experimental Teaching Center, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, People's Republic of China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Lei
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D053758:Nanoparticles / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D047392:beta-Cyclodextrins; D011096:Polygalacturonase; C031215:betadex
keywords: enzyme immobilization; pH-responsive; pectinase; self-assembly; β-cyclodextrin