title: Microbacterium horti sp. nov., a bacterium isolated from Cucurbita maxima cultivating soil.
abstract: A novel bacterial strain THG-SL1(T) was isolated from a soil sample of Cucurbita maxima garden and was characterized by using a polyphasic approach. Cells were Gram-reaction-positive, non-motile and rod-shaped. The strain was aerobic, catalase positive and weakly positive for oxidase. Phylogenetic analysis based on 16S rRNA gene sequence analysis but it shared highest similarity with Microbacterium ginsengisoli KCTC 19189(T) (96.6 %), indicating that strain THG-SL1(T) belongs to the genus Microbacterium. The DNA G + C content of the isolate was 68.9 mol %. The major fatty acids were anteiso-C15: 0 (39.7 %), anteiso-C17: 0 (24.4 %) and iso-C16: 0 (18.5 %). The major polar lipids of strain THG-SL1(T) were phosphatidylglycerol (PG) and an unidentified glycolipid (GL). The predominant respiratory isoprenoid quinones were menaquinone-11 and menaquinone-12. The diamino acid in the cell-wall peptidoglycan was ornithine. Based on the results of polyphasic characterization, strain THG-SL1(T) represented a novel species within the genus Microbacterium, for which the name Microbacterium horti sp. nov. is proposed. The type strain is THG-SL1(T) (=KACC 18286(T)=CCTCC AB 2015117(T)).
authors.affiliation: Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi, 446-701, Republic of Korea.
authors.forename: Shahina
authors.initials: S
authors.lastname: Akter
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi, 446-701, Republic of Korea.
authors.forename: Jae Hee
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, # 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. acuyin@khu.ac.kr.
authors.forename: Chang Shik
authors.initials: CS
authors.lastname: Yin
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001482:Base Composition; D028464:Cucurbita; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity; D024482:Vitamin K 2 / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2
keywords: 16S rRNA; Gram-reaction-positive; Microbacterium horti; Polyphasic taxonomy