title: Developmental validation of the PowerPlex(®) Fusion 6C System.
abstract: The PowerPlex(®) Fusion 6C System is a 27-locus, six-dye, multiplex that includes all markers in the expanded CODIS core loci and increases overlap with STR database standards throughout the world. Additionally, it contains two, rapidly mutating, Y-STRs and is capable of both casework and database workflows, including direct amplification. A multi-laboratory developmental validation study was performed on the PowerPlex(®) Fusion 6C System. Here, we report the results of that study which followed SWGDAM guidelines and includes data for: species specificity, sensitivity, stability, precision, reproducibility and repeatability, case-type samples, concordance, stutter, DNA mixtures, and PCR-based procedures. Where appropriate we report data from both extracted DNA samples and direct amplification samples from various substrates and collection devices. Samples from all studies were separated on both Applied Biosystems 3500 series and 6-dye capable 3130 series Genetic Analyzers and data is reported for each. Together, the data validate the design and demonstrate the performance of the PowerPlex(®) Fusion 6C System.
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: martin.ensenberger@promega.com.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Ensenberger
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
authors.forename: Kristy A
authors.initials: KA
authors.lastname: Lenz
authors.affiliation: Los Angeles County Sheriff's Department Crime Laboratory, 1800 Paseo Racho Castilla, Los Angeles, CA 90032, USA.
authors.forename: Learden K
authors.initials: LK
authors.lastname: Matthies
authors.affiliation: Los Angeles County Sheriff's Department Crime Laboratory, 1800 Paseo Racho Castilla, Los Angeles, CA 90032, USA.
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Hadinoto
authors.affiliation: Connecticut Department of Emergency Services and Public Protection, Division of Scientific Services, DNA Unit, 278 Colony Street, Meriden, CT 06451, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Schienman
authors.affiliation: Connecticut Department of Emergency Services and Public Protection, Division of Scientific Services, DNA Unit, 278 Colony Street, Meriden, CT 06451, USA.
authors.forename: Angela J
authors.initials: AJ
authors.lastname: Przech
authors.affiliation: Connecticut Department of Emergency Services and Public Protection, Division of Scientific Services, DNA Unit, 278 Colony Street, Meriden, CT 06451, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Morganti
authors.affiliation: Connecticut Department of Emergency Services and Public Protection, Division of Scientific Services, DNA Unit, 278 Colony Street, Meriden, CT 06451, USA.
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Renstrom
authors.affiliation: Connecticut Department of Emergency Services and Public Protection, Division of Scientific Services, DNA Unit, 278 Colony Street, Meriden, CT 06451, USA.
authors.forename: Victoria M
authors.initials: VM
authors.lastname: Baker
authors.affiliation: Erie County (NY) Central Police Services Forensic Laboratory, 45 Elm Street, 417E, Buffalo, NY 14203, USA.
authors.forename: Kori M
authors.initials: KM
authors.lastname: Gawrys
authors.affiliation: Minnesota Bureau of Criminal Apprehension, 1430 Maryland Avenue East, St. Paul, MN 55106, USA.
authors.forename: Marlijn
authors.initials: M
authors.lastname: Hoogendoorn
authors.affiliation: National Institute of Standards and Technology, Applied Genetics Group, 100 Bureau Drive, Gaithersburg, MD 20899, USA.
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Steffen
authors.affiliation: National Institute of Toxicology and Forensic Sciences, Madrid Department, Biology Service, José Echegaray 4, Las Rozas 28232, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Martín
authors.affiliation: National Institute of Toxicology and Forensic Sciences, Madrid Department, Biology Service, José Echegaray 4, Las Rozas 28232, Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Alonso
authors.affiliation: North Dakota Office of Attorney General, Crime Laboratory Division, 2641 East Main Avenue, Bismarck, ND 58501, USA.
authors.forename: Hope R
authors.initials: HR
authors.lastname: Olson
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Sprecher
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Storts
mesh_terms: D000818:Animals; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis / Q000235:genetics; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D044707:Forensic Sciences / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D004247:DNA
keywords: CODIS; DNA typing; Forensic science; Fusion 6C; PowerPlex(®); STR; Short tandem repeat; Validation