title: [Study on Stability of Human Intestinal Bacterial Biotransformation Model].
abstract: Objective: To investigate the stability of human intestinal bacterial biotransformation model using isoquercetin as the substrate of transformation. METHODs: The in vitro transformation model was established using the intestinal bacteria form different volunteers, or different passages of the same volunteer in accordance with the "biotransformation model of human intestinal bacteria and its standard operating procedures" to transform isoquercetin.
  
  
  RESULTS
  Within 24 hours, all models established with the intestinal bacteria from different volunteers could transform isoquercetin to quercetin and the transformation efficiency was inclined to increase with the increase of the number of culture passages.
  
  
  CONCLUSIONS
  The intestinal bacterial model established in accordance with the "standard operating procedures" is stable and the results obtained with this model are reproducible, which demonstrats the suitability of this model for the investigation of the chemical constituents of Chinese medicinal materials.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Cong
authors.forename: Ru-feng
authors.initials: RF
authors.lastname: Wang
authors.forename: Yu-shuai
authors.initials: YS
authors.lastname: Peng
authors.forename: Li-jia
authors.initials: LJ
authors.lastname: Liu
authors.forename: Can
authors.initials: C
authors.lastname: Zhao
authors.forename: Xiu-wei
authors.initials: XW
authors.lastname: Yang
mesh_terms: D001711:Biotransformation; D004365:Drugs, Chinese Herbal; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D011794:Quercetin / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D011794:Quercetin