title: Multimodal formyl peptide receptor 1 targeted inflammation imaging probe: cFLFLF-MHI-DOTA.
abstract: Formyl peptide receptor 1 (FPR1) targeting multimodal probe cFLFLFK-MHI-DOTA for leukocyte based inflammation imaging is described. The compound consists of three domains, (a) cFLFLF peptide for FPR1 recognition and binding for activated leukocyte, (b) heptamethine cyanine dye (MHI) for near infrared fluorescence (NIRF) detection and imaging, and (c) metal chelator DOTA ligand that could form complex with a radiometal for nuclear (PET/SPECT) imaging or with a paramagnetic metal for MRI imaging. Detailed synthesis, characterization and in vitro evaluation are reported. The availability of dual mode inflammation imaging probe would allow in vivo gross level imaging of inflammation foci as well as ex vivo microscopic level cellular imaging for role played by innate immune cells in inflamed tissue.
authors.affiliation: Department of Radiology & Medical Imaging, University of Virginia, Charlottesville, VA 22908, United States; School of Materials and Science & Engineering, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Radiology & Medical Imaging, University of Virginia, Charlottesville, VA 22908, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiology & Medical Imaging, University of Virginia, Charlottesville, VA 22908, United States.
authors.forename: Mahendra D
authors.initials: MD
authors.lastname: Chordia
authors.affiliation: The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Hospital for Special Surgery, New York, NY 10021, United States.
authors.forename: Li
authors.initials: L
authors.lastname: Shao
authors.affiliation: Department of Radiology & Medical Imaging, University of Virginia, Charlottesville, VA 22908, United States. Electronic address: dp3r@virginia.edu.
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Pan
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging*; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008670:Metals / Q000737:chemistry; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry*; D049268:Positron-Emission Tomography; D011859:Radiography; D044042:Receptors, Formyl Peptide / Q000737:chemistry / Q000378:metabolism*; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D002232:Carbocyanines; D003287:Contrast Media; C469986:FPR1 protein, human; D008670:Metals; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; C000605614:cinnamoyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanine
keywords: Formyl peptide receptor; Inflammation; Molecular imaging; Near infrared fluorescence; cFLFLF peptide