title: Caveolin-1 affects tumor drug resistance in esophageal squamous cell carcinoma by regulating expressions of P-gp and MRP1.
abstract: Esophageal squamous cell carcinoma (ESCC) is the most common cancer in China, and multidrug resistance (MDR) remains one of the biggest problems in ESCC chemotherapy. In this study, we aimed to investigate the mechanism of Caveolin-1, an integral membrane protein, on regulating ESCC MDR. First, immunohistochemistry was used to check the protein expression of Caveolin-1, MDR-related protein of P-glycoprotein (P-gp), and multidrug resistance protein 1 (MRP1) in 84 pathologically characterized ESCC tissues, matched adjacent tumor, and adjacent normal-looking tissues. The results showed that Caveolin-1 expression level was elevated in ESCC tissues than that of matched adjacent tumor and adjacent normal-looking tissues (P < 0.05), and the expression of Caveolin-1 has close correlation with P-gp and MRP1 during tumor genesis of ESCC (P = 0.034, P = 0.009, respectively). Then, Caveolin-1 overexpression and knockdown were used to investigate its effect on expressions of P-gp and MRP1 in ESCC cell line Ec9706. The messenger RNA (mRNA) and protein expression levels of P-gp and MRP1 were checked by real-time quantitative reverse transcription-PCR (qRT-PCR) and Western blot (WB). The results showed that Caveolin-1 overexpression significantly promotes the mRNA and protein expression of MRP1 (P < 0.05), while almost has no effect on the mRNA and protein expression of P-gp (P > 0.05); Cavoelin-1 knockdown inhibits the mRNA and protein expressions of both P-gp and MRP1 (P < 0.05). The similar result was found in another ESCC cell line Eca109. So, it is concluded that Caveolin-1 affects ESCC MDR by regulating the expressions of P-gp and MRP1; therefore, it can be taken as a significant marker and target in tumor therapy.
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Basic Science of Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, 450001, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Cao
authors.affiliation: Henan Tianxing Education and Media Company, Limited, Zhengzhou, Henan Province, 450002, China.
authors.forename: Mingjin
authors.initials: M
authors.lastname: Yue
authors.affiliation: Department of Basic Science of Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, 450001, China.
authors.forename: Naigang
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Department of Basic Science of Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, 450001, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Basic Science of Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, 450001, China.|Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan Province, 450001, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Basic Science of Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, 450001, China.|Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan Province, 450001, China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Dong
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. wlx2246@126.com.
authors.forename: Shixin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Basic Science of Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, 450001, China. sjmo@zzu.edu.cn.|Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan Province, 450001, China. sjmo@zzu.edu.cn.
authors.forename: Saijun
authors.initials: S
authors.lastname: Mo
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D051242:Caveolin 1 / Q000235:genetics*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D051242:Caveolin 1; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; C495540:CAV1 protein, human; C421109:multidrug resistance-associated protein 1
keywords: Caveolin-1; Esophageal squamous cell carcinoma; MRP1; Multidrug resistance; P-gp