title: Hedgehog Signaling Regulates the Ciliary Transport of Odorant Receptors in Drosophila.
abstract: Hedgehog (Hh) signaling is a key regulatory pathway during development and also has a functional role in mature neurons. Here, we show that Hh signaling regulates the odor response in adult Drosophila olfactory sensory neurons (OSNs). We demonstrate that this is achieved by regulating odorant receptor (OR) transport to and within the primary cilium in OSN neurons. Regulation relies on ciliary localization of the Hh signal transducer Smoothened (Smo). We further demonstrate that the Hh- and Smo-dependent regulation of the kinesin-like protein Cos2 acts in parallel to the intraflagellar transport system (IFT) to localize ORs within the cilium compartment. These findings expand our knowledge of Hh signaling to encompass chemosensory modulation and receptor trafficking.
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Gonzalo M
authors.initials: GM
authors.lastname: Sanchez
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Liza
authors.initials: L
authors.lastname: Alkhori
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Hatano
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Sebastian W
authors.initials: SW
authors.lastname: Schultz
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Anujaianthi
authors.initials: A
authors.lastname: Kuzhandaivel
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Shadi
authors.initials: S
authors.lastname: Jafari
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Granseth
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden. Electronic address: mattias.alenius@liu.se.
authors.forename: Mattias
authors.initials: M
authors.lastname: Alenius
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002118:Calcium / Q000378:metabolism; D002923:Cilia / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism; D016296:Mutagenesis; D009812:Odorants; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000072086:Smoothened Receptor
chemical_list: D029721:Drosophila Proteins; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D018035:Receptors, Odorant; D000072086:Smoothened Receptor; C107452:cos protein, Drosophila; C070844:ptc protein, Drosophila; C100664:smo protein, Drosophila; D016547:Kinesins; D002118:Calcium