title: Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene.
abstract: Industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. In the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt medium was assessed. 16S rRNA sequence analysis showed that they belong to four bacterial genera, namely Acinetobacter, Bacillus, Microbacterium, and Ochrobactrum. Among these isolates, Bacillus megaterium YB3 showed considerably good growth and was further evaluated for its pyrene-degrading efficiency. B. megaterium YB3 could degrade 72.44% of 500 mg L(-1) pyrene within 7 days. GC-MS analysis of ethyl acetate extracted fractions detected two relatively less toxic metabolic intermediates of the pyrene degradation pathway. B. megaterium YB3 also tested positive for catechol 1, 2-dioxygenase and aromatic-ring-hydroxylating dioxygenase indole-indigo conversion assays. Considering the ability and efficiency of B. megaterium YB3 to degrade high pyrene content, the strain can be used as a tool to develop bioremediation technologies for the effective biodegradation of pyrene and possibly other PAHs in the environment.
authors.affiliation: University School of Biotechnology, Guru Gobind Singh Indraprastha University, Dwarka, New Delhi, India.
authors.forename: Sumer Singh
authors.initials: SS
authors.lastname: Meena
authors.affiliation: Department of Environmental Studies, University of Delhi, Delhi, India.
authors.forename: Radhey Shyam
authors.initials: RS
authors.lastname: Sharma
authors.affiliation: State Grading Laboratory, Directorate of Agricultural Marketing, Govt. of NCT of Delhi, Delhi, India.
authors.forename: Priti
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Department of Environmental Studies, University of Delhi, Delhi, India.
authors.forename: Swagata
authors.initials: S
authors.lastname: Karmakar
authors.affiliation: University School of Biotechnology, Guru Gobind Singh Indraprastha University, Dwarka, New Delhi, India.
authors.forename: Kamal Krishan
authors.initials: KK
authors.lastname: Aggarwal
mesh_terms: D000085:Acetates / Q000737:chemistry; D001410:Bacillus megaterium / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase / Q000032:analysis; D049308:Dioxygenases / Q000032:analysis; D004789:Enzyme Activation; D007203:Indigo Carmine / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D000085:Acetates; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C007650:ethyl acetate; D007203:Indigo Carmine; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase
keywords: Bacillus megaterium; Biodegradation; Metabolites; PAHs; Pyrene