title: Complex Enzyme-Assisted Extraction, Purification, and Antioxidant Activity of Polysaccharides from the Button Mushroom, Agaricus bisporus (Higher Basidiomycetes).
abstract: Agaricus bisporus polysaccharides (ABP) were extracted by complex enzyme-assisted extraction methodology. The following were optimal conditions for the extraction of crude ABP: complex enzyme amount, 2.2%; temperature, 62°C; time, 3 h; and pH, 4. Under these conditions, the experimental yield of crude ABP was 6.87%. The crude ABP was purified by diethylaminoethyl-cellulose 52 chromatography and Sephadex G-100 chromatography, and one fraction-namely, ABP-1-was produced. The ABP-1 contained 93.67% carbohydrate, 1.46% protein, and 0.62% uronic acid. The constituent monosaccharides were predominantly glucose, galactose, mannose, and xylose. The antioxidant activities of ABP-1 were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, its ferric-reducing activity power, and the reducing power assay. At a concentration of 1.2 mg/mL, ABP-1 seemed to possess good free radical scavenging activity, with a scavenging value of about 56%. The results indicate that ABP-1 has good antioxidant activity.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China.
authors.forename: Xiulian
authors.initials: X
authors.lastname: Yin
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China; Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals & Oils, Nanjing University of Finance and Economics, Nanjing, China.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: You
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China.
authors.forename: Xinghai
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013696:Temperature
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl