title: Predictive value of plasma miRNA-718 for esophageal squamous cell carcinoma.
abstract: BACKGROUND
  Increasing evidence demonstrated that circulating miRNAs could serve as meaningfully non-invasive and reliable biomarkers for the detection of various cancers.
  
  
  OBJECTIVE
  To investigate the expression level of plasma miRNA-718 in esophageal squamous cell carcinoma (ESCC) patients and its diagnostic value.
  
  
  METHODS
  Quantitative real time polymerase chain reaction (qRT-PCR) method was performed to examine the expression levels of plasma miRNA-718 in 120 consecutive ESCC patients and 51 healthy controls. The difference of plasma miRNA-718 expression level between the paired pre- and postoperative patients was compared. The correlation between plasma miRNA-718 expression levels and clinicopathological characteristics was further analyzed and the receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic power of plasma miRNA-718 for ESCC.
  
  
  RESULTS
  Plasma miRNA-718 expression level of ESCC patients was significantly lower than that of healthy controls. Compared with preoperative patients, the plasma miRNA-718 expression level of postoperative patients was significantly upregulated. Plasma miRNA-718 expression level was inversely correlated with the lymph node metastasis and TNM stage. The ROC curve analysis showed that plasma miRNA-718 yielded AUCs of 0.715, 0.689 and 0.620 for the detection of ESCC patients, early patients with Tis-T1 or early patients with TNM 0-I, respectively.
  
  
  CONCLUSIONS
  Plasma miRNA-718 is downregulated in ESCC patients and might serve as a potential diagnostic marker for ESCC.
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Dong
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Kaiyan
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Meng
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Lv
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ming
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.|Henan Key Laboratory of Medical Laboratory, Zhengzhou, Henan, China.
authors.forename: Fucheng
authors.initials: F
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; C000589574:MIRN718 microRNA, human; D035683:MicroRNAs
keywords: Esophageal squamous cell carcinoma; diagnosis; microRNA-718; plasma