title: Down regulation of miR-18a, miR-21 and miR-221 genes in gastric cancer cell line by chrysin-loaded PLGA-PEG nanoparticles.
abstract: Chrysin were well-documented as having significant biological roles particularly cancer chemo-preventive activity. However, the poor water solubility of chrysin limited their bioavailability and biomedical applications. In this study, we encapsulate the chrysin into PLGA-PEG nanoparticles for local treatment. In regard to the amount of the drug load, IC50 was significant decreased in nanocapsulated chrysin in comparison with free chrysin. This was confirmed through decrease of miR-18a, miR-21, and miR-221 genes expression by real-time PCR. The results demonstrated that PLGA-PEG-chrysin complexes can be more effective than free chrysin. Therefore, PLGA-PEG can be a better nanocarrier for this kind of hydrophobic flavonoid.
authors.affiliation: a Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|b Hematology and Oncology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|c International Aras Branch, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Mohammadian
authors.affiliation: a Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Yones
authors.initials: Y
authors.lastname: Pilehvar-Soltanahmadi
authors.affiliation: a Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mofarrah
authors.affiliation: a Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dastani-Habashi
authors.affiliation: a Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|b Hematology and Oncology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D035683:MicroRNAs / Q000096:biosynthesis*; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D005419:Flavonoids; D019344:Lactic Acid; D035683:MicroRNAs; D011092:Polyethylene Glycols; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012334:RNA, Neoplasm; C522576:MIRN18A microRNA, human; C524940:MIRN21 microRNA, human; C525709:MIR221, human; C043561:chrysin; C000595209:polyethylene glycol 1000
keywords: Chrysin; PLGA-PEG; miRNAs; real-time PCR