title: Combination antiretroviral studies for patients with primary biliary cirrhosis.
abstract: Following the characterization of a human betaretrovirus in patients with primary biliary cirrhosis (PBC), pilot studies using antiretroviral therapy have been conducted as proof of principal to establish a link of virus with disease and with the eventual aim to find better adjunct therapies for patients unresponsive to ursodeoxycholic acid. In the first open label pilot study, the reverse transcriptase inhibitor lamivudine had little demonstrable biochemical or histological effect after 1 year. Whereas, lamivudine in combination with zidovudine was associated with a significant reduction in alkaline phosphatase as well as improvement in necroinflammatory score, cholangitis and ductopenia over a 12 mo period. A double blind, multi-center randomized controlled trial using lamivudine with zidovudine for 6 mo confirmed a significant reduction in alkaline phosphatase, ALT and AST in patients on antiviral therapy. However, none of the patients achieved the stringent endpoint criteria for normalization of alkaline phosphatase. Furthermore, some patients developed biochemical rebound consistent with drug resistance. A major fault of these studies has been the inability to measure the viral load in peripheral blood and therefore, provide a direct correlation between improvement of hepatic biochemistry and reduction in viral load. Nevertheless, viral mutants to lamivudine with zidovudine were later characterized in the NOD.c3c4 mouse model of PBC that has been used to test other antiretroviral regimens to betaretrovirus. The combination of tenofovir and emtricitabine reverse transcriptase inhibitors and the HIV protease inhibitor, lopinavir were found to abrogate cholangitis in the NOD.c3c4 mouse model and the same regimen normalized the liver tests in a PBC patient with HIV and human betaretrovirus infection. This combination antiretroviral therapy has now been used in a double blind randomized controlled crossover study for patients with PBC followed by an open label extension study. Only a third of the PBC patients were able to tolerate the lopinavir but those maintained on tenofovir, emtricitabine and lopinavir experienced sustained and clinically meaningful reduction in hepatic biochemistry. While we await the histological and virological evaluation, it is clear that better tolerated regimens of antiretroviral treatment will be required in future clinical trials.
authors.affiliation: Ellina Lytvyak, Aldo J Montano-Loza, Andrew L Mason, Division of Gastroenterology and Hepatology, University of Alberta, Edmonton T6G 2E1, Alberta, Canada.
authors.forename: Ellina
authors.initials: E
authors.lastname: Lytvyak
authors.affiliation: Ellina Lytvyak, Aldo J Montano-Loza, Andrew L Mason, Division of Gastroenterology and Hepatology, University of Alberta, Edmonton T6G 2E1, Alberta, Canada.
authors.forename: Aldo J
authors.initials: AJ
authors.lastname: Montano-Loza
authors.affiliation: Ellina Lytvyak, Aldo J Montano-Loza, Andrew L Mason, Division of Gastroenterology and Hepatology, University of Alberta, Edmonton T6G 2E1, Alberta, Canada.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Mason
mesh_terms: D000818:Animals; D044966:Anti-Retroviral Agents / Q000008:administration & dosage*; D030121:Betaretrovirus / Q000302:isolation & purification / Q000472:pathogenicity; D002986:Clinical Trials as Topic; D004359:Drug Therapy, Combination; D000068679:Emtricitabine / Q000008:administration & dosage; D006801:Humans; D019259:Lamivudine / Q000008:administration & dosage; D008105:Liver Cirrhosis, Biliary / Q000188:drug therapy* / Q000821:virology; D061466:Lopinavir / Q000008:administration & dosage; D008324:Mammary Tumor Virus, Mouse / Q000472:pathogenicity; D051379:Mice; D000068698:Tenofovir / Q000008:administration & dosage; D015215:Zidovudine / Q000008:administration & dosage
chemical_list: D044966:Anti-Retroviral Agents; D061466:Lopinavir; D019259:Lamivudine; D015215:Zidovudine; D000068698:Tenofovir; D000068679:Emtricitabine
keywords: Antiretroviral therapy; Endpoints for trials; Human betaretrovirus; Primary biliary cirrhosis; Randomized controlled trial