title: Preparative separation of gallocatechin gallate from Camellia ptilophylla using macroporous resins followed by sephadex LH-20 column chromatography.
abstract: Gallocatechin gallate (GCG) possesses multiple potential biological activities. However, the content of GCG in traditional green tea is too low which limits its in-depth pharmacological research and application. In the present study, a simple, efficient and environment-friendly chromatographic separation method was developed for preparative enrichment and separation of GCG from cocoa tea (Camellia ptilophylla) which contains high content of GCG. In the first step, the adsorption properties of selected resins were evaluated, and XAD-7HP resin was chosen by its adsorption and desorption properties for GCG. In order to maximize column efficiency for GCG collection, the operating parameters (e.g., flow rate, ethanol concentration, and bed height) were optimized. We found that the best combination was the feed concentration at 20mg/mL, flow rate at 0.75 BV/h and the ratio of diameter to bed heights as 1:12. Under these conditions, the purity of GCG was 45% with a recovery of 89%. In order to obtain pure target, a second step was established using column chromatography with sephadex LH-20 gel and 55% ethanol-water solution as eluent. After this step, the purity of the GCG was 91% with a recovery of 68% finally.
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China; CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China; Department of Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Li
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China; CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China; CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.
authors.forename: Cheuk-Lun
authors.initials: CL
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Environment Science, Yi Li Normal University, Yining, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China; CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xianggang
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Chuangxing
authors.initials: C
authors.lastname: Ye
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China; CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China. Electronic address: gohey@cuhk.edu.hk.
authors.forename: Chun-hay
authors.initials: CH
authors.lastname: Ko
mesh_terms: D028244:Camellia / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003911:Dextrans / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D003911:Dextrans; D010936:Plant Extracts; D012117:Resins, Synthetic; C417940:gallocatechin gallate; D002392:Catechin; C025614:sephadex
keywords: Camellia ptilophylla; Gallocatechin gallate; Macroporous resins; Preparative enrichment and separation; Sephadex LH-20