title: Effect of denture-coating composite on Candida albicans biofilm and surface degradation after disinfection protocol.
abstract: BACKGROUND
  Denture stomatitis is the most common pathology affecting denture wearers and its main cause is colonisation of dentures with Candida albicans.
  
  
  OBJECTIVE
  This study investigated the effectiveness of two commercial composite surface sealants (Biscover(®) LV and Surface Coat(®)) to reduce C. albicans biofilm colonisation on denture resin, as well as their surface integrity after disinfection cycles with 1% sodium hypochlorite solution.
  
  
  METHODS
  Heat-cured acrylic resin specimens were manufactured (10 mm × 10 mm × 1 mm). The specimen surfaces were mechanically polished to simulate rough or smooth denture surfaces. Four surface-treatment groups were tested: smooth surfaces [0.3 μm of mean roughness (Ra)]; rough surfaces (3 μm of Ra); rough surfaces treated with Biscover(®) LV; and rough surfaces treated with Surface Coat(®). Specimens of each group were randomly divided to undergo immersion in distilled water or 1% sodium hypochlorite for 30 or 90 cycles each. Specimens of all groups in each immersion solution were tested using a crystal violet (CV) staining assay for biofilm quantification and by scanning electron microscopy for visual analyses of surface integrity and biofilm structure. CV assay data were analysed using one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test (P < 0.05).
  
  
  RESULTS
  The effectiveness and surface integrity of Biscover(®) LV-treated surfaces were similar to those of smooth surfaces, whereas Surface Coat(®) -treated surfaces presented a similar performance to rough surfaces in all solutions and cycles.
  
  
  CONCLUSIONS
  These results suggest the possibility of clinical use of Biscover(®) LV for denture coating on surfaces in which mechanical polish is not indicated, such as the fitting surface.
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors.forename: Matheus Jacobina
authors.identifier: http://orcid.org/0000-0001-5879-1309
authors.initials: MJ
authors.lastname: Silva
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors.forename: Denise G
authors.identifier: http://orcid.org/0000-0003-1215-5299
authors.initials: DG
authors.lastname: de Oliveira
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors.forename: Oscar O
authors.initials: OO
authors.lastname: Marcillo
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors.forename: Karin H
authors.initials: KH
authors.lastname: Neppelenbroek
authors.affiliation: Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, São Paulo, Brazil.
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Lara
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors.forename: Vinícius C
authors.initials: VC
authors.lastname: Porto
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D018441:Biofilms; D002176:Candida albicans / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D003775:Dental Polishing / Q000379:methods; D003819:Denture Bases; D003820:Denture Cleansers / Q000737:chemistry; D004203:Disinfection / Q000379:methods*; D005840:Gentian Violet; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D012973:Sodium Hypochlorite / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000179:Acrylates; D000180:Acrylic Resins; C481141:BisCover; D020099:Coated Materials, Biocompatible; D004396:Coloring Agents; D003764:Dental Materials; D003820:Denture Cleansers; D019279:Resin Cements; D012464:Saliva, Artificial; D012973:Sodium Hypochlorite; D005840:Gentian Violet
keywords: Candida albicans; Dentures; crystal violet; dental polishing; denture cleansers; scanning electron microscopy