title: Coating morphology and surface composition of acrylic terpolymers with pendant catechol, OEG and perfluoroalkyl groups in varying ratio and the effect on protein adsorption.
abstract: This work aims at developing versatile low-biofouling polymeric coatings by using acrylic terpolymers (DOFs) that bear pendant catechol (D), oligo(ethylene glycol) (O), and perfluoroalkyl (F) groups in varying ratios. The polymers were endowed with the ability to form firmly coatings on virtually any surfaces and undergo surface microphase separation and self-assembly, as revealed by the surface enrichment of F pendants and the morphology variation from irregular solid domains to discrete crater-type aggregates of different size. The effect on protein adsorption was investigated using bovine serum albumin (BSA) and adhesive fibrinogen (Fib) as model proteins. The coating of DOF164 (low F content), which has morphology of discrete crater-type aggregates of ∼ 400 nm in size, adsorbed a least amount of protein but with a highest protein unit activity as determined by SPR and immunosorbent assay; whereas the coating of DOF1612 (high F content) showed a 12.3-fold higher adsorption capacity toward Fib. Interestingly, a 2.2-fold lower adsorption amount but with a 1.8-fold higher unit activity was found for Fib adsorbed on the DOF164 surface than on DOF250 (without F fraction), whose OEG segments being a widely recognized protein compatible material. The features of the DOF164 terpolymer presenting a robust coating ability and a minimal protein adsorption capacity while with a high protein unit activity suggest its potential application as a non-fouling surface-modifier for medical antifouling coatings and as a matrix material for selective protein immobilization and activity preservation in biosensor construction.
authors.affiliation: Orthopaedic Department of Renmin Hospital, Wuhan University, Wuhan 430060, PR China. Electronic address: zhongjun8496@aliyun.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Orthopaedic Department of Renmin Hospital, Wuhan University, Wuhan 430060, PR China; Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ji
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Duan
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Tu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China. Electronic address: adzhang@mail.ccnu.edu.cn.
authors.forename: Aidong
authors.initials: A
authors.lastname: Zhang
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D057219:Biofouling; D002396:Catechols / Q000737:chemistry*; D002417:Cattle; D005466:Fluorocarbons / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D000179:Acrylates; D002396:Catechols; D005466:Fluorocarbons; D011108:Polymers; D011506:Proteins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C034221:catechol
keywords: Antifouling; Catechol; Dopamine; Morphology; Perfluoroalkyl; Poly(ethylene glycol); Protein adsorption; Terpolymer