title: NMR-based metabolomic analysis of Haliotis diversicolor exposed to thermal and hypoxic stresses.
abstract: Haliotis diversicolor is a commercially important cultured shellfish. It is also an important marine model organism for environmental science. High temperature accompanied with hypoxia frequently induces diseases or even death to abalones. In present study, (1)H NMR spectroscopy together with pattern recognition methods was used to investigate the responses of muscle and gill of H. diversicolor to thermal and hypoxic stresses. It was found that obvious gender-, time- and tissue-specific metabolic responses were induced by thermal and hypoxic stresses. In combination with the changes of H. diversicolor in physiological features, the dual-modal stresses were suggested to mainly cause the disturbance in energy metabolism and osmotic balance in muscle and gill tissues with different mechanisms. Further, the corresponding correlation networks and metabolic pathways derived from the characteristic metabolites were used to assess the major metabolic functions of these characteristic metabolites. These findings shed some lights on the metabolic influences of environmental stresses on marine organisms.
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, PR China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China. Electronic address: shcai@xmu.edu.cn.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China. Electronic address: jianghua.feng@xmu.edu.cn.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Feng
mesh_terms: D000818:Animals; D004784:Environmental Monitoring; D049851:Gastropoda / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*
keywords: Gill; Haliotis diversicolor; Metabolomics; Muscle; Thermal and hypoxic stresses