title: [Vacuolating autotransporter toxin affects biological characteristics and pathogenicity of avian pathogenic Escherichia coli].
abstract: OBJECTIVE
  To determined the role of vacuolating autotransporter toxin (vat) gene in avian pathogenic Escherichia coli (APEC), we detected the biological characteristics and pathogenicity of vat gene mutant strain of APEC-O1.
  
  
  METHODS
  We constructed the vat mutant and complementary strain of APEC-O1 by the Red recombination system and plasmid pSTV28. Then we compared the growth curve, motility, agglutination, biofilm formation and pathogenicity of mutant strain, wild-type strain and complementary strain.
  
  
  RESULTS
  The vat mutant did not affect the growth and resistance to environment stress of APEC. However, inactivation of APEC-O1 vat gene resulted in enhanced motility, diminished agglutination, decreased biofilm formation and attenuated virulence in ducks.
  
  
  CONCLUSIONS
  These data indicated that Vat affect the motility, agglutination, biofilm formation and virulence of APEC-O1, which help us to understand the role of the Vat in the APEC pathogenicity.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shaohui
authors.initials: S
authors.lastname: Wang
authors.forename: Denghui
authors.initials: D
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Xiangan
authors.initials: X
authors.lastname: Han
authors.forename: Mingxing
authors.initials: M
authors.lastname: Tian
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.forename: Zongping
authors.initials: Z
authors.lastname: Liu
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D004372:Ducks; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D011201:Poultry Diseases / Q000382:microbiology*; D000069320:Type V Secretion Systems / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D000069320:Type V Secretion Systems; C000605763:vacuolating autotransporter toxin, E coli