title: A Mutant with Expression Deletion of Gene Sec-1 in a 1RS.1BL Line and Its Effect on Production Quality of Wheat.
abstract: The chromosome arm 1RS of rye (Secale cereal L.) has been used worldwide as a source of genes for agronomic and resistant improvement. However, the 1RS arm in wheat has end-use quality defects that are partially attributable to the presence of ω-secalins, which are encoded by genes at the Sec-1 locus. Various attempts in removing the Sec-1 genes from the 1RS.1BL translocation chromosome have been made. In the present study, two new primary 1RS.1BL translocation lines, T917-26 and T917-15, were developed from a cross between wheat variety "A42912" and Chinese local rye "Weining." The lines T917-15 and T917-26 carried a pair of intact and homogeneous 1RS.1BL chromosomes. The line T917-26 also harbored an expression deletion of some genes at the Sec-1 locus, which originated from a mutation that occurred simultaneously with wheat-rye chromosome translocations. These results suggest that the accompanying mutations of the evolutionarily significant translocations are remarkable resources for plant improvement. Comparison of translocation lines with its wheat parent showed improvements in the end-use quality parameters, which included protein content (PC), water absorption (WA), sodium dodecyl sulfate sedimentation (SDSS), wet gluten (WG), dry gluten (DG) and dough stickiness (DS), whereas significant reduction in gluten index (GI) and stability time (ST) were observed. These findings indicate that 1RS in wheat has produced a higher amount of protein, although these comprised worse compositions. However, in the T917-26 line that harbored an expression deletion mutation in the Sec-1 genes, the quality parameters were markedly improved relative to its sister line, T917-15, especially for GI and DS (P < 0.05). These results indicated that expression deletion of Sec-1 genes significantly improves the end-use quality of wheat cultivars harboring the 1RS.1BL translocation. Strategies to remove the Sec-1 genes from the 1RS.1BL translocation in wheat improvement are discussed.
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Tianheng
authors.initials: T
authors.lastname: Ren
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Benju
authors.initials: B
authors.lastname: Yan
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Feiquan
authors.initials: F
authors.lastname: Tan
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Manyu
authors.initials: M
authors.lastname: Yang
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Ren
mesh_terms: D032461:Chromosomes, Plant; D002523:Edible Grain / Q000737:chemistry / Q000235:genetics; D005433:Flour / Q000032:analysis; D005504:Food Analysis; D063427:Food Quality; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D017384:Sequence Deletion; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins