title: Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT.
abstract: Cadherin-12 (CDH12) is a subtype of N-cadherin family. In this study, we investigated the expression of CDH12 and the role of CDH12 in prognosis of colorectal cancer (CRC) patients. In addition, we observed the influence of CDH12 on proliferation and progression of CRC cell lines. By using immunohistochemical staining, we analyzed CRC samples and adjacent non-tumor tissues collected from 78 patients who underwent laparoscopic surgery in Shanghai Minimally Invasive Center, China. Statistical analyses were used to analyze relationship between CDH12 and tumor features. Kaplan-Meier method was used to analyze patients' survival. Proliferation ability of CRC cells was tested by CCK-8 assay, and transwell assays were performed to detect migration and invasion ability. Western blot assay was performed to investigate epithelial-mesenchymal transition (EMT) variants. We found that expression of CDH12 in tumor tissue was higher than in adjacent normal tissue. High expression of CDH12 was associated with tumor invasion depth and predicts poor prognosis of CRC patients. Ectopic/repressing expression of CDH12 increased/decreased the proliferation and migration ability of CRC cells. CDH12 is able to increase cancer cell migration and invasion via promoting EMT by targeting transcriptional factor Snail. These findings may conclude that CDH12 may act as a predictor in CRC patients' prognosis and an oncogene in CRC cell proliferation and migration. CDH12 may influence CRC cell progression through promoting EMT by targeting Snail. In addition, CDH12 is promoted by MCP1 through induction of MCPIP.
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.|Shanghai Institute of Digestive Surgery, Shanghai, People's Republic of China.
authors.forename: Jingkun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.|Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.|Shanghai Institute of Digestive Surgery, Shanghai, People's Republic of China.
authors.forename: Puxiongzhi
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Surgery, Munich University, Munich, Germany.
authors.forename: Hao
authors.initials: H
authors.lastname: Feng
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zong
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.|Shanghai Institute of Digestive Surgery, Shanghai, People's Republic of China.
authors.forename: Baochi
authors.initials: B
authors.lastname: Ou
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China. prozhmh@126.com.
authors.forename: Minhua
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China. luaiguo1965@163.com.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Lu
mesh_terms: D000368:Aged; D000818:Animals; D000070585:Cadherin Related Proteins; D015820:Cadherins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011379:Prognosis; D014157:Transcription Factors / Q000235:genetics
chemical_list: C442480:CDH23 protein, human; D000070585:Cadherin Related Proteins; D015820:Cadherins; D014157:Transcription Factors
keywords: Cadherin-12; Colorectal cancer; EMT; MCP1; Metastasis; Snail