title: The Impact of Dietary Energy Intake Early in Life on the Colonic Microbiota of Adult Mice.
abstract: The complex and dynamic interactions between diet, gut microbiota (GM) structure and function, and colon carcinogenesis are only beginning to be elucidated. We examined the colonic microbiota and aberrant crypt foci (ACF) in C57BL/6N female mice fed various dietary interventions (control, energy restricted and high-fat) provided during two phases (initiation and progression) of azoxymethane (AOM)-induced early colon carcinogenesis. During progression (wks. 22-60), a high-fat diet enhanced ACF formation compared to a control or energy restricted diet. In contrast, energy restriction during initiation phase (wks. 3-21) enhanced ACF burden at 60 weeks, regardless of the diet in progression phase. Alterations in GM structure during the initiation phase diet were partially maintained after changing diets during the progression phase. However, diet during the progression phase had major effects on the mucosal GM. Energy restriction in the progression phase increased Firmicutes and reduced Bacteroidetes compared to a high-fat diet, regardless of initiation phase diet, suggesting that diet may have both transient effects as well as a lasting impact on GM composition. Integration of early life and adult dietary impacts on the colonic microbial structure and function with host molecular processes involved in colon carcinogenesis will be key to defining preventive strategies.
authors.affiliation: The Ohio State University Ph.D. Program in Nutrition, The Ohio State University, Columbus, OH 43210, USA.|Division of Epidemiology, College of Public Health, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Xu
authors.affiliation: Division of Biosciences, College of Dentistry, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Galley
authors.affiliation: Division of Biosciences, College of Dentistry, The Ohio State University, Columbus, OH 43210, USA.|Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.|Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.|Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus OH 43205, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Bailey
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Thomas-Ahner
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.|Division of Medical Oncology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Steven K
authors.initials: SK
authors.lastname: Clinton
authors.affiliation: Division of Epidemiology, College of Public Health, The Ohio State University, Columbus, OH 43210, USA.|Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Olivo-Marston
mesh_terms: D000818:Animals; D001397:Azoxymethane; D041963:Bacteroidetes / Q000187:drug effects / Q000502:physiology; D031204:Caloric Restriction; D002273:Carcinogens; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage; D018450:Disease Progression; D002149:Energy Intake; D005260:Female; D000068536:Bacillota / Q000187:drug effects / Q000502:physiology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000187:drug effects / Q000502:physiology*
chemical_list: D002273:Carcinogens; D004041:Dietary Fats; D001397:Azoxymethane