title: Baicalein Inhibits MCF-7 Cell Proliferation In Vitro, Induces Radiosensitivity, and Inhibits Hypoxia Inducible Factor.
abstract: Hypoxia inducible factor (HIF) is a key transcription factor responsible for imparting adaptability to the cancer cells growing in tumors. HIF induces the modulation of glucose metabolism, angiogenesis, and prosurvival signaling. Therefore, HIF is one of the attractive targets to treat solid tumors. Results presented in this study indicate that Baicalein (BA) inhibits HIF stabilization and also reduces its transcription activity in MCF-7 cells in vitro. Furthermore, BA was found to have antiproliferative ability as determined by the MTT assay and clonogenic survival. BA also induces apoptosis in MCF-7 cells at the concentration of 50 µM. We also report the radiosensitization of MCF-7 cells when they are treated with BA, resulting in higher γ-radiation-induced DNA damage. BA is extensively used in Chinese medicine and is known to be nontoxic at pharmacological doses. Our studies indicate that BA is one of the attractive natural compounds suitable for further evaluation as an adjuvant therapy.
authors.affiliation: Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Gade
authors.affiliation: Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
authors.forename: Nitin Motilal
authors.initials: NM
authors.lastname: Gandhi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D053960:DNA Breaks; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000037:antagonists & inhibitors* / Q000235:genetics; D061986:MCF-7 Cells; D018517:Plant Roots / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D031364:Scutellaria baicalensis / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D051793:Hypoxia-Inducible Factor 1; D011838:Radiation-Sensitizing Agents; C006680:baicalein