title: Individualized significance of the -251 A/T single nucleotide polymorphism of interleukin-8 in severe infections.
abstract: Based on the concept of the individualized nature of sepsis, we investigated the significance of the -251 A/T (rs4073) single nucleotide polymorphism (SNP) of interleukin (IL)-8 in relation to the underlying infection. Genotyping was performed in 479 patients with severe acute pyelonephritis (UTI, n = 146), community-acquired pneumonia (CAP, n = 109), intra-abdominal infections (IAI, n = 119), and primary bacteremia (BSI, n = 105) by restriction fragment length polymorphism of the polymerase chain reaction (PCR) product and compared with 104 healthy volunteers. Circulating IL-8 was measured within the first 24 h of diagnosis by an immunosorbent assay. Carriage of the AA genotype was protective from the development of UTI (odds ratio 0.38, p: 0.007) and CAP (odds ratio 0.30, p: 0.004), but not from IAI and BSI. Protection from the development of severe sepsis/septic shock was provided for carriers of the AA genotype among patients with UTI (odds ratio 0.15, p: 0.015). This was accompanied by greater concentrations of circulating IL-8 among patients with the AA genotype. It is concluded that carriage of rs4073 modifies susceptibility for severe infection in an individualized way. This is associated with a modulation of circulating IL-8.
authors.affiliation: 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece.
authors.forename: M D
authors.initials: MD
authors.lastname: Georgitsi
authors.affiliation: 1st Department of Internal Medicine, Thriasion Elefsis General Hospital, Elefsis, Greece.
authors.forename: V
authors.initials: V
authors.lastname: Vitoros
authors.affiliation: Department of Urology, Lamia General Hospital, Lamia, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Panou
authors.affiliation: 2nd Department of Critical Care Medicine, University of Athens, Medical School, Athens, Greece.
authors.forename: I
authors.initials: I
authors.lastname: Tsangaris
authors.affiliation: Intensive Care Unit, Aghios Dimitrios General Hospital, Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Aimoniotou
authors.affiliation: Department of Medicine and Research Laboratory of Internal Medicine, Medical School, University of Thessaly, Larissa, Greece.
authors.forename: N K
authors.initials: NK
authors.lastname: Gatselis
authors.affiliation: Intensive Care Unit, "G.Gennimatas" General Hospital, Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Chasou
authors.affiliation: Department of Critical Care Medicine, University of Thrace, Medical School, Alexandroupolis, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Kouliatsis
authors.affiliation: 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Leventogiannis
authors.affiliation: Department of Internal Medicine, University of Patras, Patras, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Velissaris
authors.affiliation: Department of Clinical Microbiology, Evangelismos General Hospital, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Belesiotou
authors.affiliation: Intensive Care Unit, Aghios Pavlos General Hospital, Thessaloniki, Greece.
authors.forename: O
authors.initials: O
authors.lastname: Dioritou-Aggaliadou
authors.affiliation: 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Giannitsioti
authors.affiliation: Department of Internal Medicine, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: M G
authors.initials: MG
authors.lastname: Netea
authors.affiliation: 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece. egiamarel@med.uoa.gr.|4th Department of Internal Medicine, ATTIKON University General Hospital, 1 Rimini Street, 12462, Athens, Greece. egiamarel@med.uoa.gr.
authors.forename: E J
authors.initials: EJ
authors.lastname: Giamarellos-Bourboulis
authors.affiliation: Department of Internal Medicine, Chios General Hospital, Chios, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Giannikopoulos
authors.affiliation: 2nd Department of Internal Medicine, Thriasion Elefsis General Hospital, Elefsis, Greece.
authors.forename: Z
authors.initials: Z
authors.lastname: Alexiou
authors.affiliation: 2nd Department of Surgery, Aristotle University of Thessaloniki, Medical School, Thessaloniki, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Voloudakis
authors.affiliation: 1st Department of Respiratory Medicine, University of Athens, Medical School, Athens, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Koutsoukou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001424:Bacterial Infections / Q000235:genetics* / Q000473:pathology*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D016209:Interleukin-8