title: Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil.
abstract: The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7 mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.
authors.affiliation: a Department of Agroenvironmental Chemistry and Plant Nutrition , Czech University of Life Sciences Prague , Prague , Czech Republic.
authors.forename: Jiřina
authors.initials: J
authors.lastname: Száková
authors.affiliation: a Department of Agroenvironmental Chemistry and Plant Nutrition , Czech University of Life Sciences Prague , Prague , Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Havlíčková
authors.affiliation: a Department of Agroenvironmental Chemistry and Plant Nutrition , Czech University of Life Sciences Prague , Prague , Czech Republic.
authors.forename: Adéla
authors.initials: A
authors.lastname: Šípková
authors.affiliation: b Institute of Microbiology, Vídeňská , Prague , Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Gabriel
authors.affiliation: b Institute of Microbiology, Vídeňská , Prague , Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Švec
authors.affiliation: b Institute of Microbiology, Vídeňská , Prague , Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Baldrian
authors.affiliation: c Atomic Absorption Spectrometry Laboratory, Institute of Chemical Technology , Prague , Czech Republic.
authors.forename: Jiřina
authors.initials: J
authors.lastname: Sysalová
authors.affiliation: d Department of Chemistry, Faculty of Science , Masaryk University , Brno , Czech Republic.|e Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic , Brno , Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Coufalík
authors.affiliation: d Department of Chemistry, Faculty of Science , Masaryk University , Brno , Czech Republic.
authors.forename: Rostislav
authors.initials: R
authors.lastname: Červenka
authors.affiliation: d Department of Chemistry, Faculty of Science , Masaryk University , Brno , Czech Republic.|f Department of Public Health, Faculty of Medicine , Masaryk University , Brno , Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Zvěřina
authors.affiliation: d Department of Chemistry, Faculty of Science , Masaryk University , Brno , Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Komárek
authors.affiliation: a Department of Agroenvironmental Chemistry and Plant Nutrition , Czech University of Life Sciences Prague , Prague , Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Tlustoš
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D004784:Environmental Monitoring / Q000379:methods; D004787:Environmental Pollution / Q000032:analysis*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D017669:Mercury Compounds / Q000032:analysis* / Q000737:chemistry; D008767:Methylmercury Compounds / Q000032:analysis* / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic
chemical_list: D017669:Mercury Compounds; D008767:Methylmercury Compounds; D012989:Soil Pollutants; D008628:Mercury
keywords: Mercury; microbial community; mobility; rhizobox; speciation