title: Enamel Surface with Pit and Fissure Sealant Containing 45S5 Bioactive Glass.
abstract: Enamel demineralization adjacent to pit and fissure sealants leads to the formation of marginal caries, which can necessitate the replacement of existing sealants. Dental materials with bioactive glass, which releases ions that inhibit dental caries, have been studied. The purpose of this study was to evaluate the enamel surface adjacent to sealants containing 45S5 bioactive glass (BAG) under simulated microleakage between the material and the tooth in a cariogenic environment. Sealants containing 45S5BAG filler were prepared as follows: 0% 45S5BAG + 50.0% glass (BAG0 group), 12.5% 45S5BAG + 37.5% glass (BAG12.5 group), 25.0% 45S5BAG + 25.0% glass (BAG25.0 group), 37.5% 45S5BAG + 12.5% glass (BAG37.5 group), and 50.0% 45S5BAG + 0% glass (BAG50.0 group). A cured sealant disk was placed over a flat bovine enamel disk, separated by a 60-µm gap, and immersed in lactic acid solution (pH 4.0) at 37 °C for 15, 30, and 45 d. After the storage period, each enamel disk was separated from the cured sealant disk, and the enamel surface was examined with optical 3-dimensional surface profilometer, microhardness tester, and scanning electron microscopy. The results showed a significant increase in roughness and a decrease in microhardness of the enamel surface as the proportion of 45S5BAG decreased (P< 0.05). In the scanning electron microscopy images, enamel surfaces with BAG50.0 showed a smooth surface, similar to those in the control group with distilled water, even after prolonged acid storage. Additionally, an etched pattern was observed on the surface of the demineralized enamel with a decreasing proportion of 45S5BAG. Increasing the 45S5BAG filler contents of the sealants had a significant impact in preventing the demineralization of the enamel surface within microgaps between the material and the tooth when exposed to a cariogenic environment. Therefore, despite some marginal leakage, these novel sealants may be effective preventive dental materials for inhibiting secondary caries at the margins.
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: S-Y
authors.initials: SY
authors.lastname: Yang
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: J-S
authors.initials: JS
authors.lastname: Kwon
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: K-N
authors.initials: KN
authors.lastname: Kim
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea kmkim@yuhs.ac.
authors.forename: K-M
authors.initials: KM
authors.lastname: Kim
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002326:Cariogenic Agents / Q000737:chemistry; D002417:Cattle; D002516:Ceramics / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry; D003743:Dental Enamel / Q000648:ultrastructure*; D003763:Dental Leakage / Q000473:pathology; D005898:Glass / Q000737:chemistry*; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D021621:Imaging, Three-Dimensional / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D061848:Optical Imaging / Q000379:methods; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D017001:Tooth Demineralization / Q000473:pathology
chemical_list: D002326:Cariogenic Agents; C500418:Clinpro Sealant; D003188:Composite Resins; D010895:Pit and Fissure Sealants; D011109:Polymethacrylic Acids; C425330:bioactive glass 45S5; C020946:triethylene glycol dimethacrylate; D019344:Lactic Acid; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate
keywords: SEM; caries resistance; microhardness; microleakage; surface roughness; tooth demineralization