title: The Oligo Fucoidan Inhibits Platelet-Derived Growth Factor-Stimulated Proliferation of Airway Smooth Muscle Cells.
abstract: In the pathogenesis of asthma, the proliferation of airway smooth muscle cells (ASMCs) is a key factor in airway remodeling and causes airway narrowing. In addition, ASMCs are also the effector cells of airway inflammation. Fucoidan extracted from marine brown algae polysaccharides has antiviral, antioxidant, antimicrobial, anticlotting, and anticancer properties; however, its effectiveness for asthma has not been elucidated thus far. Platelet-derived growth factor (PDGF)-treated primary ASMCs were cultured with or without oligo-fucoidan (100, 500, or 1000 µg/mL) to evaluate its effects on cell proliferation, cell cycle, apoptosis, and Akt, ERK1/2 signaling pathway. We found that PDGF (40 ng/mL) increased the proliferation of ASMCs by 2.5-fold after 48 h (p < 0.05). Oligo-fucoidan reduced the proliferation of PDGF-stimulated ASMCs by 75%-99% after 48 h (p < 0.05) and induced G₁/G₀ cell cycle arrest, but did not induce apoptosis. Further, oligo-fucoidan supplementation reduced PDGF-stimulated extracellular signal-regulated kinase (ERK1/2), Akt, and nuclear factor (NF)-κB phosphorylation. Taken together, oligo-fucoidan supplementation might reduce proliferation of PDGF-treated ASMCs through the suppression of ERK1/2 and Akt phosphorylation and NF-κB activation. The results provide basis for future animal experiments and human trials.
authors.affiliation: Department of Internal Medicine, Kuang-Tien General Hospital, No. 117, Shatian Road Shalu District, Taichung City 433, Taiwan. yan5104@ms23.hinet.net.
authors.forename: Chao-Huei
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Department of Biotechnology, Hungkuang University, 34 Chung-Chie Rd, Sha Lu, Taichung 443, Taiwan. 717230@gmail.com.
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Tsao
authors.affiliation: Department of Internal Medicine, Kuang-Tien General Hospital, No. 117, Shatian Road Shalu District, Taichung City 433, Taiwan. ker200448@yahoo.com.tw.|Institute of BioMedical Nutrition, Hungkuang University, 34 Chung-Chie Rd, Sha Lu, Taichung 443, Taiwan. ker200448@yahoo.com.tw.
authors.forename: Wang-Sheng
authors.initials: WS
authors.lastname: Ko
authors.affiliation: Institute of BioMedical Nutrition, Hungkuang University, 34 Chung-Chie Rd, Sha Lu, Taichung 443, Taiwan. chiouyl@sunrise.hk.edu.tw.|Department of Nursing, Hungkuang University, 34 Chung-Chie Rd, Sha Lu, Taichung 443, Taiwan. chiouyl@sunrise.hk.edu.tw.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Chiou
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001249:Asthma / Q000188:drug therapy / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008168:Lung / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D000459:Phaeophyceae; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats
chemical_list: D018927:Anti-Asthmatic Agents; D010982:Platelet-Derived Growth Factor; D011134:Polysaccharides; C007789:fucoidan
keywords: airway smooth muscle cells; asthma; oligo-fucoidan