title: Vitronectin: a promising breast cancer serum biomarker for early diagnosis of breast cancer in patients.
abstract: Breast cancer is the most common cancer in women worldwide, identification of new biomarkers for early diagnosis and detection will improve the clinical outcome of breast cancer patients. In the present study, we determined serum levels of vitronectin (VN) in 93 breast cancer patients, 30 benign breast lesions, 9 precancerous lesions, and 30 healthy individuals by enzyme-linked immunosorbent assays. Serum VN level was significantly higher in patients with stage 0-I primary breast cancer than in healthy individuals, patients with benign breast lesion or precancerous lesions, as well as those with breast cancer of higher stages. Serum VN level was significantly and negatively correlated with tumor size, lymph node status, and clinical stage (p < 0.05 in all cases). In addition, VN displayed higher area under curve (AUC) value (0.73, 95 % confidence interval (CI) [0.62-0.84]) than carcinoembryonic antigen (CEA) (0.64, 95 % CI [0.52-0.77]) and cancer antigen 15-3 (CA 15-3) (0.69, 95 % CI [0.58-0.81]) when used to distinguish stage 0-I cancer and normal control. Importantly, the combined use of three biomarkers yielded an improvement in receiver operating characteristic curve with an AUC of 0.83, 95 % CI [0.74-0.92]. Taken together, our current study showed for the first time that serum VN is a promising biomarker for early diagnosis of breast cancer when combined with CEA and CA15-3.
authors.affiliation: Department of Breast Surgery, Affiliated 307 Hospital, 8, Dongda Avenue, Beijing, 100071, China.
authors.forename: Wende
authors.initials: W
authors.lastname: Hao
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Bingshui
authors.initials: B
authors.lastname: Xiu
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Xiqin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Shuofeng
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Cuimi
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Breast Surgery, Affiliated 307 Hospital, 8, Dongda Avenue, Beijing, 100071, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Ying
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Breast Surgery, Affiliated 307 Hospital, 8, Dongda Avenue, Beijing, 100071, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Breast Surgery, Affiliated 307 Hospital, 8, Dongda Avenue, Beijing, 100071, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Hao
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Olympia Diagnostics, Inc., Sunnyvale, CA, 94086, USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Johnson
authors.affiliation: Department of Breast Surgery, Affiliated 307 Hospital, 8, Dongda Avenue, Beijing, 100071, China.
authors.forename: Di
authors.initials: D
authors.lastname: Meng
authors.affiliation: Division of Experimental Cancer Research, Department of Laboratory Medicine in Malmö, Clinical Research Center, Lund University, 205 02, Malmö, Sweden.
authors.forename: Jenny L
authors.initials: JL
authors.lastname: Persson
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.
authors.forename: Heqiu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China. xyfeng2002@126.com.
authors.forename: XiaoYan
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Breast Surgery, Affiliated 307 Hospital, 8, Dongda Avenue, Beijing, 100071, China. hy1963@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000097:blood; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000097:blood; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008875:Middle Aged; D011379:Prognosis; D019096:Vitronectin / Q000097:blood*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D019096:Vitronectin
keywords: Breast cancer; Diagnosis; Early stage; Serum