title: First genetic characterization of rotavirus C in Russia.
abstract: Rotaviruses C (RVC) cause sporadic cases and outbreaks of diarrhea in humans and animals worldwide. The aim of this study was to monitor RVC during a surveillance study of sporadic cases of viral gastroenteritis in the Novosibirsk and Omsk regions of Russia from 2006 to 2011. A total of 2144 stool samples from children and adults hospitalized with acute gastroenteritis were tested for RVC by RT-PCR. Sixteen RVC-positive stool samples were detected at a rate of 0.6% (13/2037) in children and 2.8% (3/107) in adults. The low detection rate suggested that RVC infection was an uncommon cause of hospitalization in Russia. The complete VP7, VP4, VP6, and NSP4 gene sequences were determined. It was found that RVCs with at least two different genome backgrounds circulated in Siberia. VP4, VP6, and NSP4 gene sequences of most Russian RVC strains clustered with South Asian strains, while the VP7 gene showed a closer relationship to European strains. Meanwhile, only VP4 and NSP4 sequences of the strain Omsk08-386 clustered with South Asian strains, while its VP6 and VP7 sequences clustered with European strains. This is the first genetic characterization of Russian RVC strains and the first report on the prevalence of RVC in the Asian part of Russia.
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. Electronic address: ezhr@niboch.nsc.ru.
authors.forename: Elena
authors.initials: E
authors.lastname: Zhirakovskaia
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
authors.forename: Artem
authors.initials: A
authors.lastname: Tikunov
authors.affiliation: Municipal Children's Hospital No. 3, Novosibirsk, Russia.
authors.forename: Vera
authors.initials: V
authors.lastname: Klemesheva
authors.affiliation: Center for Hygiene and Epidemiology in the Omsk Region, Omsk, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Loginovskikh
authors.affiliation: Novosibirsk State University, Novosibirsk, Russia. Electronic address: nauka@nsc.ru.
authors.forename: Sergey
authors.initials: S
authors.lastname: Netesov
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. Electronic address: tikunova@niboch.nsc.ru.
authors.forename: Nina
authors.initials: N
authors.lastname: Tikunova
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007297:Inpatients; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D058974:Phylogeography; D011159:Population Surveillance; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology*; D012426:Russia / Q000453:epidemiology; D055815:Young Adult
keywords: Gastroenteritis; Genotyping; Phylogenetic analysis; Prevalence; Rotavirus C