title: Protein aggregate turbidity: Simulation of turbidity profiles for mixed-aggregation reactions.
abstract: Due to their colloidal nature, all protein aggregates scatter light in the visible wavelength region when formed in aqueous solution. This phenomenon makes solution turbidity, a quantity proportional to the relative loss in forward intensity of scattered light, a convenient method for monitoring protein aggregation in biochemical assays. Although turbidity is often taken to be a linear descriptor of the progress of aggregation reactions, this assumption is usually made without performing the necessary checks to provide it with a firm underlying basis. In this article, we outline utilitarian methods for simulating the turbidity generated by homogeneous and mixed-protein aggregation reactions containing fibrous, amorphous, and crystalline structures. The approach is based on a combination of Rayleigh-Gans-Debye theory and approximate forms of the Mie scattering equations.
authors.affiliation: Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia; Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: damien.hall@anu.edu.au.
authors.forename: Damien
authors.initials: D
authors.lastname: Hall
authors.affiliation: Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Dehlsen
authors.affiliation: Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia.
authors.forename: Evelyn Jane
authors.initials: EJ
authors.lastname: Mannix
authors.affiliation: Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Williams
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan; Advanced Materials Science Center, College of Bioresource Sciences, Nihon University, Chiyoda-ku, Tokyo 102-8275, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Arisaka
authors.affiliation: Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Carver
mesh_terms: D008027:Light; D009391:Nephelometry and Turbidimetry; D066329:Protein Aggregates; D011506:Proteins / Q000737:chemistry*
chemical_list: D066329:Protein Aggregates; D011506:Proteins
keywords: Light scattering; Mixed aggregation; Protein aggregation; Turbidity