title: Chlorogenic acid ameliorates endotoxin-induced liver injury by promoting mitochondrial oxidative phosphorylation.
abstract: Acute or chronic hepatic injury is a common pathology worldwide. Mitochondrial dysfunction and the depletion of adenosine triphosphate (ATP) play important roles in liver injury. Chlorogenic acids (CGA) are some of the most abundant phenolic acids in human diet. This study was designed to test the hypothesis that CGA may protect against chronic lipopolysaccharide (LPS)-induced liver injury by modulating mitochondrial energy generation. CGA decreased the activities of serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. The contents of ATP and adenosine monophosphate (AMP), as well as the ratio of AMP/ATP, were increased after CGA supplementation. The activities of enzymes that are involved in glycolysis were reduced, while those of enzymes involved in oxidative phosphorylation were increased. Moreover, phosphorylated AMP-activated protein kinase (AMPK), and mRNA levels of AMPK-α, peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α), nuclear respiratory factor 1, and mitochondrial DNA transcription factor A were increased after CGA supplementation. Collectively, these findings suggest that the hepatoprotective effect of CGA might be associated with enhanced ATP production, the stimulation of mitochondrial oxidative phosphorylation and the inhibition of glycolysis.
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China; College of Food Safety, Guizhou Medical University, Guiyang 550025, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China. Electronic address: ruanzheng@ncu.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Xugang
authors.initials: X
authors.lastname: Shu
authors.affiliation: College of Food Safety, Guizhou Medical University, Guiyang 550025, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Mi
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Food Science and Technology and School of Food Science, Nanchang University, Nanchang 330047, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China. Electronic address: yinyulong@isa.ac.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000284:Administration, Oral; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*; D002726:Chlorogenic Acid / Q000008:administration & dosage*; D019587:Dietary Supplements; D004731:Endotoxins; D004734:Energy Metabolism / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D004731:Endotoxins; D002726:Chlorogenic Acid; D000255:Adenosine Triphosphate
keywords: Chlorogenic acid; Energy metabolism; Glycolysis; Hepatoprotective; Mitochondrial respiration