title: Coupling curvature-dependent and shear stress-stimulated neotissue growth in dynamic bioreactor cultures: a 3D computational model of a complete scaffold.
abstract: The main challenge in tissue engineering consists in understanding and controlling the growth process of in vitro cultured neotissues toward obtaining functional tissues. Computational models can provide crucial information on appropriate bioreactor and scaffold design but also on the bioprocess environment and culture conditions. In this study, the development of a 3D model using the level set method to capture the growth of a microporous neotissue domain in a dynamic culture environment (perfusion bioreactor) was pursued. In our model, neotissue growth velocity was influenced by scaffold geometry as well as by flow- induced shear stresses. The neotissue was modeled as a homogenous porous medium with a given permeability, and the Brinkman equation was used to calculate the flow profile in both neotissue and void space. Neotissue growth was modeled until the scaffold void volume was filled, thus capturing already established experimental observations, in particular the differences between scaffold filling under different flow regimes. This tool is envisaged as a scaffold shape and bioprocess optimization tool with predictive capacities. It will allow controlling fluid flow during long-term culture, whereby neotissue growth alters flow patterns, in order to provide shear stress profiles and magnitudes across the whole scaffold volume influencing, in turn, the neotissue growth.
authors.affiliation: Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Onderwijs en Navorsing 1 (+8), Herestraat 49, PB 813, 3000, Leuven, Belgium. yguyot@ulg.ac.be.|Biomechanics Research Unit, Universite de Liege, Chemin des Chevreuils 1 - BAT 52/3, 4000, Liège, Belgium. yguyot@ulg.ac.be.
authors.forename: Y
authors.initials: Y
authors.lastname: Guyot
authors.affiliation: Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Onderwijs en Navorsing 1 (+8), Herestraat 49, PB 813, 3000, Leuven, Belgium.|Skeletal Biology and Engineering Research Center, KU Leuven, Onderwijs en Navorsing 1 (+8), Herestraat 49, PB 813, 3000, Leuven, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Papantoniou
authors.affiliation: Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Onderwijs en Navorsing 1 (+8), Herestraat 49, PB 813, 3000, Leuven, Belgium.|Skeletal Biology and Engineering Research Center, KU Leuven, Onderwijs en Navorsing 1 (+8), Herestraat 49, PB 813, 3000, Leuven, Belgium.
authors.forename: F P
authors.initials: FP
authors.lastname: Luyten
authors.affiliation: Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Onderwijs en Navorsing 1 (+8), Herestraat 49, PB 813, 3000, Leuven, Belgium.|Biomechanics Research Unit, Universite de Liege, Chemin des Chevreuils 1 - BAT 52/3, 4000, Liège, Belgium.|Department of Mechanical Engineering, Biomechanics Section, KU Leuven, Celestijnenlaan 300C, PB 2419, 3001, Leuven, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Geris
mesh_terms: D001696:Biomechanical Phenomena; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D003198:Computer Simulation; D006801:Humans; D008954:Models, Biological; D013314:Stress, Mechanical; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: 3D neotissue growth; Bioreactor; Fluid flow; Numerical modeling; Shear stress influence