title: Isolation and fine mapping of Rps6: an intermediate host resistance gene in barley to wheat stripe rust.
abstract: We uncouple host and nonhost resistance in barley to Puccinia striiformis ff. spp. hordei and tritici . We isolate, fine map, and physically anchor Rps6 to chromosome 7H in barley. A plant may be considered a nonhost of a pathogen if all known genotypes of a plant species are resistant to all known isolates of a pathogen species. However, if a small number of genotypes are susceptible to some known isolates of a pathogen species this plant may be considered an intermediate host. Barley (Hordeum vulgare) is an intermediate host for Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. We wanted to understand the genetic architecture underlying resistance to Pst and to determine whether any overlap exists with resistance to the host pathogen, Puccinia striiformis f. sp. hordei (Psh). We mapped Pst resistance to chromosome 7H and show that host and intermediate host resistance is genetically uncoupled. Therefore, we designate this resistance locus Rps6. We used phenotypic and genotypic selection on F2:3 families to isolate Rps6 and fine mapped the locus to a 0.1 cM region. Anchoring of the Rps6 locus to the barley physical map placed the region on a single fingerprinted contig spanning a physical region of 267 kb. Efforts are now underway to sequence the minimal tiling path and to delimit the physical region harboring Rps6. This will facilitate additional marker development and permit identification of candidate genes in the region.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Dawson
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.|School of Biological Sciences, University of Essex, Colchester, CO4 3SQ, UK.
authors.forename: John N
authors.initials: JN
authors.lastname: Ferguson
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Matthew
authors.initials: M
authors.lastname: Gardiner
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Phon
authors.initials: P
authors.lastname: Green
authors.affiliation: National Institute of Agricultural Botany, Huntingdon Road, Cambridge, CB3 0LE, UK.
authors.forename: Amelia
authors.initials: A
authors.lastname: Hubbard
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK. matthew.moscou@sainsbury-laboratory.ac.uk.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Moscou
mesh_terms: D000704:Analysis of Variance; D001487:Basidiomycota; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers