title: Application of cell and biomaterial-based tissue engineering methods in the treatment of cartilage, menisci and ligament injuries.
abstract: Over 20 years ago it was realized that the traditional methods of the treatment of injuries to joint components: cartilage, menisci and ligaments, did not give satisfactory results and so there is a need of employing novel, more effective therapeutic techniques. Recent advances in molecular biology, biotechnology and polymer science have led to both the experimental and clinical application of various cell types, adapting their culture conditions in order to ensure a directed differentiation of the cells into a desired cell type, and employing non-toxic and non-immunogenic biomaterial in the treatment of knee joint injuries. In the present review the current state of knowledge regarding novel cell sources, in vitro conditions of cell culture and major important biomaterials, both natural and synthetic, used in cartilage, meniscus and ligament repair by tissue engineering techniques are described, and the assets and drawbacks of their clinical application are critically evaluated.
authors.affiliation: Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956, No 135/147, 61-545, Poznan, Poland. doktortrzeciak@gmail.com.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Trzeciak
authors.affiliation: Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956, No 135/147, 61-545, Poznan, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Richter
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Garbary 15, 61-688, Poznan, Poland.
authors.forename: Wiktoria
authors.initials: W
authors.lastname: Suchorska
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Garbary 15, 61-688, Poznan, Poland.|The Postgraduate School of Molecular Medicine, Medical University of Warsaw, Zwirki i Wigury 61, 02-091, Warsaw, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Augustyniak
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Garbary 15, 61-688, Poznan, Poland.|The Postgraduate School of Molecular Medicine, Medical University of Warsaw, Zwirki i Wigury 61, 02-091, Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Lach
authors.affiliation: Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956, No 135/147, 61-545, Poznan, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kaczmarek
authors.affiliation: Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956, No 135/147, 61-545, Poznan, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kaczmarczyk
mesh_terms: D001672:Biocompatible Materials / Q000627:therapeutic use*; D002356:Cartilage / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods; D006801:Humans; D007718:Knee Injuries / Q000628:therapy*; D007719:Knee Joint / Q000601:surgery; D008022:Ligaments; D019637:Orthopedic Procedures / Q000009:adverse effects / Q000379:methods*; D011108:Polymers; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers
keywords: Biocompatible; Biodegradation; Cartilage tissue engineering; Collagen; Fibrin; Scaffold; Silk