title: Well-Defined Macromolecules Using Horseradish Peroxidase as a RAFT Initiase.
abstract: Enzymatic catalysis and control over macromolecular architectures from reversible addition-fragmentation chain transfer polymerization (RAFT) are combined to give a new method of making polymers. Horseradish peroxidase (HRP) is used to catalytically generate radicals using hydrogen peroxide and acetylacetone as a mediator. RAFT is used to control the polymer structure. HRP catalyzed RAFT polymerization gives acrylate and acrylamide polymers with relatively narrow molecular weight distributions. The polymerization is rapid, typically exceeding 90% monomer conversion in 30 min. Complex macromolecular architectures including a block copolymer and a protein-polymer conjugate are synthesized using HRP to catalytically initiate RAFT polymerization.
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Alex P
authors.initials: AP
authors.lastname: Danielson
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Dylan
authors.initials: D
authors.lastname: Bailey-Van Kuren
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Melissa E
authors.initials: ME
authors.lastname: Lucius
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Makaroff
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Cameron
authors.initials: C
authors.lastname: Williams
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Page
authors.affiliation: Department of Chemical, Paper and Biomedical Engineering, Miami University, 650 E High St, Oxford, OH, 45056, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Berberich
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
authors.forename: Dominik
authors.initials: D
authors.lastname: Konkolewicz
mesh_terms: D055162:Biocatalysis; D005609:Free Radicals / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007700:Kinetics; D015394:Molecular Structure; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005609:Free Radicals; D011108:Polymers; D006735:Horseradish Peroxidase
keywords: enzyme catalysis; heme proteins; polymerization; protein modifications; raft polymerization