title: Keratinocytes derived from chicken embryonic stem cells support Marek's disease virus infection: a highly differentiated cell model to study viral replication and morphogenesis.
abstract: BACKGROUND
  Marek's disease is a virus disease with worldwide distribution that causes major losses to poultry production. Vaccines against Marek's disease virus, an oncogenic alphaherpesvirus, reduce tumour formation but have no effect on virus shedding. Successful horizontal virus transmission is linked to the active viral replication in feather follicle epithelial cells of infected chickens, from which infectious viral particles are shed into the environment. The feather follicle epithelium is the sole tissue in which those infectious particles are produced and no in vitro cell-systems can support this highly efficient morphogenesis. We previously characterized embryonic stem-cell-derived keratinocytes, showing they display a marker-gene profile similar to skin keratinocytes, and therefore we tested their susceptibility to Marek's disease virus infection.
  
  
  RESULTS
  We show herein that keratinocytes derived from chicken embryonic stem-cells are fully permissive to the replication of either non-pathogenic or pathogenic Marek's disease viruses. All viruses replicated on all three keratinocyte lines and kinetics of viral production as well as viral loads were similar to those obtained on primary cells. Morphogenesis studies were conducted on infected keratinocytes and on corneocytes, showing that all types of capsids/virions were present inside the cells, but extracellular viruses were absent.
  
  
  CONCLUSIONS
  The keratinocyte lines are the first epithelial cell-line showing ectodermal specific markers supporting Marek's disease virus replication. In this in vitro model the replication lead to the production of cell-associated viral progeny. Further work will be devoted to the study of relationship between 3D differentiation of keratinocytes and Marek's disease virus replication.
authors.affiliation: INRA - Université François-Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, ISP, F-37380, Nouzilly, France. mathilde.couteaudier@hotmail.fr.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Couteaudier
authors.affiliation: INRA - Université François-Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, ISP, F-37380, Nouzilly, France. Katia.Courvoisier@tours.inra.fr.
authors.forename: Katia
authors.initials: K
authors.lastname: Courvoisier
authors.affiliation: INRA - Université François-Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, ISP, F-37380, Nouzilly, France. Laetitia.Fragnet@tours.inra.fr.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Trapp-Fragnet
authors.affiliation: INRA - Université François-Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, ISP, F-37380, Nouzilly, France. Caroline.Denesvre@tours.inra.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: Denesvre
authors.affiliation: INRA - Université François-Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, ISP, F-37380, Nouzilly, France. Jean-Francois.Vautherot@tours.inra.fr.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Vautherot
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D053595:Embryonic Stem Cells / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology* / Q000821:virology*; D025922:Mardivirus / Q000502:physiology* / Q000648:ultrastructure; D008380:Marek Disease / Q000821:virology; D014779:Virus Replication