title: Zinc acclimation mitigated high zinc induced oxidative stress by enhancing antioxidant defenses in large yellow croaker Pseudosciaena crocea.
abstract: The hypothesis tested in the present study was that Zn acclimation will alleviate high Zn induced oxidative stress in large yellow croaker Pseudosciaena crocea. To the end, fish were pre-exposed to 0 and 2mgZnL(-1) for 48h and then exposed to 0 and 10mgZnL(-1) for 48h. Lipid peroxidation, activities and mRNA levels of antioxidant enzyme genes (Cu/Zn-SOD, CAT, GPx and GR), and gene expressions of Nrf2-Keap1 signaling molecules at different exposure time (12h, 24h and 48h) were determined in the liver and spleen of large yellow croaker. 10mgZnL(-1) exposure alone enhanced lipid peroxidation in the liver during 12-48h and in the spleen during 24-48h. Although 2mgZnL(-1) pre-exposure did not affect lipid peroxidation, 2mgZnL(-1) pre-exposure mitigated high Zn induced oxidative stress. The positive effect of Zn acclimation could be attributed to the up-regulated expression and activities of antioxidant enzyme genes under high Zn stress. Obtained results also showed a coordinated transcriptional regulation of antioxidant genes, suggesting that Nrf2 is required for the protracted induction of these genes. Besides, the sharp increase in Keap1 expression levels would support its role in switching off Nrf2 response. In conclusion, Zn acclimation mitigated high Zn-induced oxidative stress in large yellow croker, emphasizing a central role of transcription factor Nrf2 in the process.
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China. Electronic address: zhengjialang@aliyun.com.
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
authors.affiliation: Postgraduate Work Department, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Zhu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Wu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Ai-Yi
authors.initials: AY
authors.lastname: Zhu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shen
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000818:Animals; D029941:Fish Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010473:Perciformes / Q000378:metabolism / Q000502:physiology*; D013154:Spleen / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015032:Zinc / Q000378:metabolism* / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; D015032:Zinc
keywords: Antioxidant response; Gene expression; Large yellow croaker; Nrf2; Zinc acclimation