title: Molecular characterization and expression patterns of emerin (EMD) gene in skeletal muscle between Meishan and Large White pigs.
abstract: The emerin protein is a nuclear membrane protein and has important functions in muscle development, regeneration, and cell signal transduction. However, knowledge regarding emerin in the domestic animal is limited. In this study, we cloned and characterized the pig emerin (EMD) gene. Semi-quantitative RT-PCR analysis revealed that the EMD gene was expressed at the highest level in the heart and fat at 120d. However, the fetal skeletal muscles displayed a greater abundance of EMD mRNA than that in skeletal muscles at postnatal development stages. In addition, the expression level of EMD at 60 day was significantly higher (p<0.05) in Meishan than Large White pigs. Pig EMD protein displayed the sarcolemma and perinuclear distribution in skeletal muscle sections, and there was no distribution change of EMD in skeletal muscle sections between Large White and Meishan pigs. These studies provide useful information for further research on the functions of pig EMD gene in skeletal muscle.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University, Chengdu, Sichuan, PR China. Electronic address: wanglinjie@sicau.edu.cn.
authors.forename: Linjie
authors.initials: L
authors.lastname: Wang
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000196:embryology* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism; D034421:Sus scrofa / Q000196:embryology / Q000235:genetics*
chemical_list: D008565:Membrane Proteins; D009687:Nuclear Proteins; C092710:emerin
keywords: Different expression; Emerin; Pig; Skeletal muscle