title: Rifoligotyping assay: an alternative method for rapid detection of rifampicin resistance inMycobacterium tuberculosisisolates from Morocco.
abstract: One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug resistant strains. In the past decades, considerable efforts have been made upon the development of new molecular technologies and methodologies for detection of drug resistance inMycobacterium tuberculosis(MTB). A sensitive, specific reverse line blot assay, called rifoligotyping (RIFO), for the detection of genotypic resistance to rifampicin (RIF), was designed and evaluated. RIFO includes oligonucleotide probes specific for wild-type and mutant sequences, allowing specific and sensitive detection of both genotypes in a single assay. The RIFO was applied on 500 MTB isolates from Morocco. The results of the RIFO showed a good sensitivity (90.9%) and high specificity (100%); the positive and negative predictive values were 100% and 96.1%, respectively. This rapid, simple, economical assay provides a practical alternative for RIF genotyping, especially in low-income countries, to improve TB control and management.
authors.affiliation: Unit of Biology and Medical Research, National Center of Energy, Sciences and Nuclear Techniques, Rabat, Morocco; Laboratory of Biochimestry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco.
authors.forename: Imane
authors.initials: I
authors.lastname: Chaoui
authors.affiliation: Unit of Biology and Medical Research, National Center of Energy, Sciences and Nuclear Techniques , Rabat , Morocco.
authors.forename: Naima
authors.initials: N
authors.lastname: Atalhi
authors.affiliation: National Tuberculosis Reference Laboratory, National Institute of Hygiene , Rabat , Morocco.
authors.forename: Radia
authors.initials: R
authors.lastname: Sabouni
authors.affiliation: National Tuberculosis Reference Laboratory, National Institute of Hygiene , Rabat , Morocco.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Akrim
authors.affiliation: Laboratory of Mycobacteria Genetics, Research Service, Pasteur Institute , Tanger , Morocco.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Abid
authors.affiliation: Laboratory of Biochimestry and Immunology, Faculty of Sciences, Mohammed V University , Rabat , Morocco.
authors.forename: Saaid
authors.initials: S
authors.lastname: Amzazi
authors.affiliation: Unit of Biology and Medical Research, National Center of Energy, Sciences and Nuclear Techniques , Rabat , Morocco.
authors.forename: Mohammed
authors.initials: M
authors.lastname: ElMzibri
keywords: MTB; Morocco; multi drug resistant; reverse line blot assay; rifampicin resistance