title: Cell membrane chromatography coupled with UHPLC-ESI-MS/MS method to screen target components from Peucedanum praeruptorum Dunn acting on α1A adrenergic receptor.
abstract: Peucedanum praeruptorum Dunn (BaiHuaQianHu in Chinese) is a traditional Chinese medicine that has a long history of use in China. In this study, HEK 293 α1A adrenergic cell membrane chromatography was coupled with UHPLC-ESI-MS/MS and successfully used to identify active components from Peucedanum praeruptorum Dunn. Paeruptorin A, paeruptorin B, and paeruptorin C were identified with α1A adrenergic receptor activity. Pharmacological assays showed that tamsulosin hydrochloride, paeruptorin A, paeruptorin B, and paeruptorin C in concentrations of 1×10(-8) to 1×10(-4)mol/mL could relax prostate strips pre-contracted with adrenalin in a concentration dependent manner. Therefore, the HEK293 α1A cell membrane chromatography coupled UHPLC-ESI-MS/MS system may be a potentially useful drug discovery method for screening for medicinal herbal components with α1A adrenergic receptor inhibitory activity.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, Shaanxi, PR China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Pharmacy, Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, PR China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, Shaanxi, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, Shaanxi, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, Shaanxi, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, Shaanxi, PR China. Electronic address: wangsc@mail.xjtu.edu.cn.
authors.forename: Sicen
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D018340:Receptors, Adrenergic, alpha-1
keywords: Cell membrane chromatography; Peucedanum praeruptorum Dunn; UHPLC–ESI–MS/MS; α1A adrenergic receptor