title: A stereospecific solid-phase screening assay for colonies expressing both (R)- and (S)-selective ω-aminotransferases.
abstract: A novel solid-phase screening assay was developed for colonies expressing both (R)- and (S)-selective ω-aminotransferases. This high-throughput assay can be used to screen rapidly large variant libraries with enhanced substrate selectivity and enantioselectivities.
authors.affiliation: School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Willies
authors.affiliation: School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK.
authors.forename: James L
authors.initials: JL
authors.lastname: Galman
authors.affiliation: School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK.
authors.forename: Iustina
authors.initials: I
authors.lastname: Slabu
authors.affiliation: School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK nicholas.turner@manchester.ac.uk.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turner
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D001681:Biological Assay / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D013237:Stereoisomerism; D000637:Transaminases
chemical_list: D000637:Transaminases
keywords: aminotransferases; biocatalysis; directed evolution