title: Investigation of FIH-1 and SOCS3 expression in KRAS mutant and wild-type patients with colorectal cancer.
abstract: Colorectal cancer (CRC) is a multistep process based on the accumulation of somatic mutations in genes such as APC and KRAS. Data on the presence of mutations in KRAS gene in CRC and its relationship with clinicopathological parameters and expression of genes involved in tumor progression are scarce. We unbiasedly examined the KRAS status in samples from 99 patients and its correlation with clinicopathological parameters such as age, sex, tumor location, lymph node metastasis, tumor stage, tumor grade, and vascular invasion. Consistent with reports of other researchers, 38.4 % of our samples harbored KRAS mutation in their genomes with preferential mutation in codon 12 (89.4 %). Nevertheless, unlike previous reports, we were not able to correlate KRAS status with clinicopathological parameters (P > 0.05) except for vascular invasion. Patients with KRAS mutation have more vascular invasion compared with patient having wild-type KRAS. Next, we investigated the expression of two tumor suppressor genes, factor-inhibiting hypoxia-inducible factor 1 (FIH-1) and suppressor of cytokine signaling (SOCS3), in both KRAS mutant and wild-type groups and looked for any correlation between their expression and clinicopathological parameters. Although the expression of both genes was not regular, none of the clinicopathological parameters were associated with the expressions of FIH-1 and SOCS3 at mRNA level (P > 0.05). However, decline in FIH-1 expression at protein level in KRAS mutant group was correlated with stage IV and grade 2 of tumor (P ≤ 0.05). Our results demonstrated that there is no or low correlation between KRAS status, FIH-1, and SOCS3 expression with epidemiologic and clinicpathological characteristics in CRC.
authors.affiliation: Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR, Iran.|Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR, Iran.|GI and Liver Disease Research Center, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Vakil
authors.affiliation: Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR, Iran. rnajafipour@gmail.com.|Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR, Iran. rnajafipour@gmail.com.
authors.forename: Reza
authors.initials: R
authors.lastname: Najafipour
authors.affiliation: GI and Liver Disease Research Center, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran. n_rakhshani@yahoo.com.
authors.forename: Nasser
authors.initials: N
authors.lastname: Rakhshani
authors.affiliation: GI and Liver Disease Research Center, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Zamani
authors.affiliation: Molecular Pathology Department of Mehr General Hospital, Tehran, Iran.
authors.forename: Arman
authors.initials: A
authors.lastname: Morakabati
authors.affiliation: Departments of Medical Informatics, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Javadi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003062:Codon / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000235:genetics*
chemical_list: D003062:Codon; C117307:KRAS protein, human; D012097:Repressor Proteins; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D006899:Mixed Function Oxygenases; C439790:HIF1AN protein, human; D016283:Proto-Oncogene Proteins p21(ras)
keywords: Colorectal cancer; FIH-1 and SOCS3; KRAS mutation