title: Complete genome sequence of Methanospirillum hungatei type strain JF1.
abstract: Methanospirillum hungatei strain JF1 (DSM 864) is a methane-producing archaeon and is the type species of the genus Methanospirillum, which belongs to the family Methanospirillaceae within the order Methanomicrobiales. Its genome was selected for sequencing due to its ability to utilize hydrogen and carbon dioxide and/or formate as a sole source of energy. Ecologically, M. hungatei functions as the hydrogen- and/or formate-using partner with many species of syntrophic bacteria. Its morphology is distinct from other methanogens with the ability to form long chains of cells (up to 100 μm in length), which are enclosed within a sheath-like structure, and terminal cells with polar flagella. The genome of M. hungatei strain JF1 is the first completely sequenced genome of the family Methanospirillaceae, and it has a circular genome of 3,544,738 bp containing 3,239 protein coding and 68 RNA genes. The large genome of M. hungatei JF1 suggests the presence of unrecognized biochemical/physiological properties that likely extend to the other Methanospirillaceae and include the ability to form the unusual sheath-like structure and to successfully interact with syntrophic bacteria.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA ; UCLA DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095-1489 USA.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Gunsalus
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA.
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Cook
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Crable
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA.
authors.forename: Lars
authors.initials: L
authors.lastname: Rohlin
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA.
authors.forename: Erin
authors.initials: E
authors.lastname: McDonald
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA ; Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 USA.
authors.forename: Housna
authors.initials: H
authors.lastname: Mouttaki
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 USA.
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Sieber
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Poweleit
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489 USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Theodosius Dobzhansky Center for Genome Bionformatics, St. Petersburg State University, St. Petersburg, Russia ; Algorithmic Biology Lab, St. Petersburg Academic University, St. Petersburg, Russia.
authors.forename: Alla L
authors.initials: AL
authors.lastname: Lapidus
authors.affiliation: Theodosius Dobzhansky Center for Genome Bionformatics, St. Petersburg State University, St. Petersburg, Russia.
authors.forename: Hajnalka Erzsebet
authors.initials: HE
authors.lastname: Daligault
authors.affiliation: Oak Ridge National Laboratory, Oak Ridge, Tennessee USA.
authors.forename: Miriam
authors.initials: M
authors.lastname: Land
authors.affiliation: Oak Ridge National Laboratory, Oak Ridge, Tennessee USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Gilna
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA ; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Culley
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McInerney
keywords: Anaerobic; Formate; Hydrogen; Methangenic archaea; Methanomicrobiales; Motile; Syntrophic partnerships