title: Nonlinearity in MCF7 Cell Survival Following Exposure to Modulated 6 MV Radiation Fields: Focus on the Dose Gradient Zone.
abstract: The study of cell survival following exposure to nonuniform radiation fields is taking on particular interest because of the increasing evidence of a nonlinear relationship at low doses. We conducted in vitro experiments using the MCF7 breast cancer cell line. A 2.4 × 2.4 cm(2) square area of a T25 flask was irradiated by a Varian Novalis accelerator delivering 6 MV photons. Cell survival inside the irradiation field, in the dose gradient zone and in the peripheral zone, was determined using a clonogenic assay for different radiation doses at the isocenter. Increased cell survival was observed inside the irradiation area for doses of 2, 10, and 20 Gy when nonirradiated cells were present at the periphery, while the cells at the periphery showed decreased survival compared to controls. Increased survival was also observed at the edge of the dose gradient zone for cells receiving 0.02 to 0.01 Gy when compared with cells at the periphery of the same flask, whatever the isocenter dose. These data are the first to report cell survival in the dose gradient zone. Radiotherapists must be aware of this nonlinearity in dose response.
authors.affiliation: Laboratoire d'Histologie-Embryologie, Faculté de Médecine Rangueil, Toulouse, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Lacoste-Collin
authors.affiliation: Laboratoire d'Histologie-Embryologie, Faculté de Médecine Rangueil, Toulouse, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Castiella
authors.affiliation: Equipe 15 UMR 1037, Centre de Recherche en Cancérologie de Toulouse et Service de Radiochirurgie Stéréotaxique, Centre Hospitalier Universitaire Rangueil, Toulouse, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Franceries
authors.affiliation: Equipe 15 UMR 1037, Centre de Recherche en Cancérologie de Toulouse et Service de Radiochirurgie Stéréotaxique, Centre Hospitalier Universitaire Rangueil, Toulouse, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Cassol
authors.affiliation: Service de Radiothérapie, Institut Claudius Regaud, Toulouse, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Vieillevigne
authors.affiliation: Groupement Scientifique en Biologie et Médecine Spatiale, Faculté de Médecine Rangueil, Toulouse, France.
authors.forename: Veronica
authors.initials: V
authors.lastname: Pereda
authors.affiliation: Equipe 15 UMR 1037, Centre de Recherche en Cancérologie de Toulouse et Service de Radiochirurgie Stéréotaxique, Centre Hospitalier Universitaire Rangueil, Toulouse, France.
authors.forename: Manuel
authors.initials: M
authors.lastname: Bardies
authors.affiliation: Laboratoire d'Histologie-Embryologie, Faculté de Médecine Rangueil, Toulouse, France; Groupement Scientifique en Biologie et Médecine Spatiale, Faculté de Médecine Rangueil, Toulouse, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Courtade-Saïdi
keywords: IMRT; MCF7 breast cancer cell line; bystander effect; clonogenic assay; dose gradient zone