title: Capillary electrophoresis of RNA in hydroxyethylcellulose polymer with various molecular weights.
abstract: Recent research demonstrates that large numbers of long noncoding RNAs (lncRNAs) in mammals exhibit indices of functionality, and thus analysis of longer RNAs is of great significance. In the present work, we investigated the effect of molecular weight on the separation performance of long RNA by capillary electrophoresis (CE). Results demonstrate that (1) low molecular weight of hydroxyethylcellulose (HEC) (90k) favors the separation of short RNA (<1000 nt). The resolution for short RNA was improved and the migration time was linearly extended with the increase of polymer concentration. (2) In the longer chain HEC (250k, 720k and 1300k), the resolution for the small RNA fragment (<1000 nt) became better as the polymer concentration increased, whereas the resolution for the large ones (>3000 nt) deteriorated. (3) Based on logarithmic plot, there exist two migration regimes for RNA in short chain HEC (90k), three regimes in moderate chain HEC (250k and 720k), and four regimes in the long chain HEC (1300k). Such a systematic investigation of long RNAs may be useful for research on lncRNAs in the length range of 100-10,000 nt.
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China. Electronic address: zhenqingli@163.com.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Wuhan Mechanical Technology College, No. 42 LuoYu East Road, Wuhan 430075, China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Luo
authors.affiliation: Photonics and Bio-medical Research Institute, Department of Physics Faculty of Science, East China University of Science and Technology, No. 130 Meilong Road, Shanghai 200237, China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka Suita-city, Osaka 565-0871, Japan. Electronic address: yoshi.yamaguchi@ap.eng.osaka-u.ac.jp.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D008970:Molecular Weight; D012313:RNA / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D012313:RNA; D002482:Cellulose; C002283:hydroxyethylcellulose
keywords: Capillary electrophoresis; Hydroxyethylcellulose; RNA