title: Creation of a Hybrid Scaffold with Dual Configuration of Aligned and Random Electrospun Fibers.
abstract: A novel hybrid construct was developed by combining aligned fibers (AFs) and random fibers (RFs) to form a scaffolding system. Homogeneous fiber-based structures were fabricated by electrospinning, which produced both random and aligned fiber mats depending on the collection method. The upper part of the scaffold contained an AF layer, which possessed a well-organized configuration that provided uniaxial topographic guidance. For mechanical stability and support, the lower part of the scaffold was composed of an RF layer. Despite the presence of randomly distributed RFs, desirable alignment and differentiation could be achieved in cultured C2C12 myoblasts by controlling the density of AF layer. The fibrous structure of the hybrid scaffold also exhibited high porosity and therefore reasonable permeability. Owing to the structural stability provided by the underlying RFs, the cell-laden fibrous scaffolds were amenable to physical manipulation, such as multilayering. Collectively, the morphological features and manipulable architecture of the developed scaffolds suggest that they would perform well in practical applications.
authors.affiliation: Micro/Nano Scale Manufacturing R&D Group, Korea Institute of Industrial Technology , Ansan-si, Gyeonggi-do 426-910, Korea.
authors.forename: Suk-Hee
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Mechanical and Aerospace Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Mechanical and Aerospace Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Byungjun
authors.initials: B
authors.lastname: Lee
authors.affiliation: Micro/Nano Scale Manufacturing R&D Group, Korea Institute of Industrial Technology , Ansan-si, Gyeonggi-do 426-910, Korea.
authors.forename: Jean Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Micro/Nano Scale Manufacturing R&D Group, Korea Institute of Industrial Technology , Ansan-si, Gyeonggi-do 426-910, Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Micro/Nano Scale Manufacturing R&D Group, Korea Institute of Industrial Technology , Ansan-si, Gyeonggi-do 426-910, Korea.
authors.forename: Nak Kyu
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Department of Mechanical and Aerospace Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Noo Li
authors.initials: NL
authors.lastname: Jeon
authors.affiliation: Department of Mechanical and Aerospace Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Kahp-Yang
authors.initials: KY
authors.lastname: Suh
mesh_terms: D000818:Animals; D002460:Cell Line; D048430:Cell Shape; D005455:Fluorescent Antibody Technique; D051379:Mice; D032446:Myoblasts / Q000166:cytology; D010539:Permeability; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: aligned fiber; electrospinning; muscle tissue engineering; myoblast; random fiber