title: Enhanced dynamic instability of microtubules in a ROS free inert environment.
abstract: Reactive oxygen species (ROS), one of the regulators in various biological processes, have recently been suspected to modulate microtubule (MT) dynamics in cells. However due to complicated cellular environment and unavailability of any in vitro investigation, no detail is understood yet. Here, by performing simple in vitro investigations, we have unveiled the effect of ROS on MT dynamics. By studying dynamic instability of MTs in a ROS free environment and comparing with that in the presence of ROS, we disclosed that MTs showed enhanced dynamics in the ROS free environment. All the parameters that define dynamic instability of MTs e.g., growth and shrinkage rates, rescue and catastrophe frequencies were significantly affected by the presence of ROS. This work clearly reveals the role of ROS in modulating MT dynamics in vitro, and would be a great help in understanding the role of ROS in regulation of MT dynamics in cells.
authors.affiliation: Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Md Sirajul
authors.initials: MS
authors.lastname: Islam
authors.affiliation: Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Arif Md Rashedul
authors.initials: AM
authors.lastname: Kabir
authors.affiliation: Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Inoue
authors.affiliation: Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan; Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Sada
authors.affiliation: Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan; Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan. Electronic address: kakugo@sci.hokudai.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Kakugo
mesh_terms: D000818:Animals; D008870:Microtubules / Q000378:metabolism*; D017382:Reactive Oxygen Species; D013552:Swine; D014404:Tubulin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D014404:Tubulin
keywords: Dynamic instability; Microtubule; Oxidative stress; Reactive oxygen species; Total internal reflection fluorescence microscopy; Tubulin