title: APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea.
abstract: Lepidopteran midgut aminopeptidases N (APNs) are phylogenetically divided into eight clusters, designated as APN1-8. Although APN1 has been implicated as one of the receptors for Cry1Ac in several species, its potential role in the mode of action of Cry2Ab has not been functionally determined so far. To test whether APN1 also acts as one of the receptors for Cry1Ac in Helicoverpa zea and even for Cry2Ab in this species, we conducted a gain of function analysis by heterologously expressing H. zea APN1 (HzAPN1) in the midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) and a loss of function analysis by RNAi (RNA interference) silencing of the endogenous APN1 in the three cell lines using the HzAPN1 double strand RNA (dsRNA). Heterologous expression of HzAPN1 significantly increased the susceptibility of the three cell lines to Cry1Ac, but had no effects on their susceptibility to Cry2Ab. Knocking down of the endogenous APN1 made the three cell lines resistant to Cry1Ac, but didn't change cell lines susceptibility to Cry2Ab. The findings from this study demonstrate that HzAPN1 is a functional receptor of Cry1Ac, but not Cry2Ab.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.|Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721.
authors.forename: Jizhen
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Gemei
authors.initials: G
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Kongming
authors.initials: K
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: USDA-ARS, Crop Genetics and Breeding Research Unit, Tifton, GA 31793, USA.
authors.forename: Xinzhi
authors.initials: X
authors.lastname: Ni
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.|Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Li
mesh_terms: D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004731:Endotoxins / Q000378:metabolism* / Q000633:toxicity; D041981:Gastrointestinal Tract / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014162:Transfection; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D004731:Endotoxins; D000626:Aminopeptidases