title: Interaction of stromal and microvascular components in keratocystic odontogenic tumors.
abstract: OBJECTIVE
  Little is known about the interaction of stromal components in odontogenic tumors. Thus, the aim of this study was to investigate mast cells (MCs), myofibroblasts, macrophages, and their possible association with angiogenesis and lymphangiogenesis in keratocystic odontogenic tumors (KCOTs).
  
  
  METHODS
  Thirty cases of KCOTs were included and analyzed by immunohistochemistry for mast cell tryptase, α-SMA, CD34, CD163, and D240. For comparative purpose, 15 radicular cysts (CRs) and 7 pericoronal follicles (PFs) were included.
  
  
  RESULTS
  There was an increase in MCs for RCs and this difference was significant when they were compared to KCOTS and PFs. A significant increase in the density of MFs was observed for KCOTs when compared to RCs and PFs (P = 0.00). No significant difference in CD163-positive macrophages (P = 0.084) and CD34-positive vessels (P = 0.244) densities was observed between KCOTs, RCs, and PFs, although KCOTs showed a higher density of all proteins. Significant difference in lymphatic vessel density was observed for KCOTs when compared to RCs and PFs (P = 0.00). Positive correlation was observed between mast cell tryptase and CD34 in KCOTs (P = 0.025).
  
  
  CONCLUSIONS
  A significant interaction between the MC population and CD34-positive vessels in KCOTs supported the hypothesis that MCs and blood vessels contribute to the stromal scaffold of KCOT.
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Ernesto Santos
authors.initials: ES
authors.lastname: Sousa-Neto
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.|Department of Dental Public Health, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Maria Cristina Teixeira
authors.initials: MC
authors.lastname: Cangussu
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.|Human Pathology Postgraduate Program, Oswaldo Cruz Foundation, Fiocruz, Salvador, Bahia, Brazil.|Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Fiocruz, Salvador, Bahia, Brazil.
authors.forename: Clarissa Araújo
authors.identifier: http://orcid.org/0000-0001-8922-2985
authors.initials: CA
authors.lastname: Gurgel
authors.affiliation: Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Fiocruz, Salvador, Bahia, Brazil.
authors.forename: Vanessa Sousa
authors.initials: VS
authors.lastname: Guimarães
authors.affiliation: Human Pathology Postgraduate Program, Oswaldo Cruz Foundation, Fiocruz, Salvador, Bahia, Brazil.
authors.forename: Eduardo Antônio Gonçalves
authors.initials: EA
authors.lastname: Ramos
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.|Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Flávia Caló Aquino
authors.initials: FC
authors.lastname: Xavier
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.|Division of Periodontics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Patrícia Ramos
authors.initials: PR
authors.lastname: Cury
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.|Department of Oral and Maxillofacial Surgery, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Braúlio
authors.initials: B
authors.lastname: Carneiro Júnior
authors.affiliation: Department of Medical and Surgical Sciences, University of Catania, Catania, Italy.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Leonardi
authors.affiliation: Dentistry and Health Postgraduate Program, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.|Human Pathology Postgraduate Program, Oswaldo Cruz Foundation, Fiocruz, Salvador, Bahia, Brazil.|Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Jean Nunes
authors.initials: JN
authors.lastname: Dos Santos
mesh_terms: D003238:Connective Tissue / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D042583:Lymphangiogenesis; D042601:Lymphatic Vessels / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008407:Mast Cells / Q000473:pathology; D058628:Myofibroblasts / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology; D009807:Odontogenic Cysts / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009808:Odontogenic Tumors / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology*; D011842:Radicular Cyst / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*
keywords: angiogenesis; cells; keratocyst; lymphangiogenesis; macrophages; myofibroblasts