title: Studies of the viral binding proteins of shrimp BP53, a receptor of white spot syndrome virus.
abstract: The specific binding between viral attachment proteins (VAPs) of a virus and its cellular receptors on host cells mediates virus entry into host cells, which triggers subsequent viral infections. Previous studies indicate that F1 ATP synthase β subunit (named BP53), is found on the surface of shrimp cells and involved in white spot syndrome virus (WSSV) infection by functioning as a potential viral receptor. Herein, in a far-western blotting assay, three WSSV proteins with molecular weights of 28 kDa, 37 kDa, and >50 kDa were found to interact with BP53. The 28 kDa and 37 kDa proteins were identified as the envelope protein VP28 and VP37 of WSSV respectively, which could be recognized by the polyclonal antibodies. Enzyme-linked immunosorbent binding assays revealed that VP37 contributed to almost 80% of the binding capability for BP53 compared with the same amount of total WSSV protein. The relationship between BP53 and its complementary interacting protein, VP37, was visualized using a co-localization assay. Bound VP37 on the cell surface co-localized with BP53 and shared a similar subcellular location on the outer surface of shrimp cells. Pearson's correlation coefficients reached to 0.67 ± 0.05 and the Mander's overlap coefficients reached 0.70 ± 0.05, which indicated a strong relationship between the localization of BP53 and bound rVP37. This provides evidence for an interaction between BP53 and VP37 obtained at the molecular and cellular levels, supporting the hypothesis that BP53 serves as a receptor for WSSV by binding to VP37. The identification of the viral binding proteins of shrimp BP53 is helpful for better understanding the pathogenic mechanisms of WSSV to infect shrimp at the cellular level.
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Gao
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: liangyan@ysfri.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
mesh_terms: D025181:ATP Synthetase Complexes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D054884:Host-Pathogen Interactions; D033561:Penaeidae / Q000378:metabolism / Q000821:virology*; D011991:Receptors, Virus / Q000737:chemistry* / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D053585:Virus Attachment; D053586:Virus Internalization; D046848:White spot syndrome virus 1 / Q000472:pathogenicity*
chemical_list: D011991:Receptors, Virus; D017361:Viral Nonstructural Proteins; D025181:ATP Synthetase Complexes
keywords: BP53; F(1) ATP synthase β subunit; Interaction; Shrimp; VP37; WSSV