title: A novel automated device for rapid nucleic acid extraction utilizing a zigzag motion of magnetic silica beads.
abstract: We report a novel automated device for nucleic acid extraction, which consists of a mechanical control system and a disposable cassette. The cassette is composed of a bottle, a capillary tube, and a chamber. After sample injection in the bottle, the sample is lysed, and nucleic acids are adsorbed on the surface of magnetic silica beads. These magnetic beads are transported and are vibrated through the washing reagents in the capillary tube under the control of the mechanical control system, and thus, the nucleic acid is purified without centrifugation. The purified nucleic acid is automatically extracted in 3 min for the polymerase chain reaction (PCR). The nucleic acid extraction is dependent on the transport speed and the vibration frequency of the magnetic beads, and optimizing these two parameters provided better PCR efficiency than the conventional manual procedure. There was no difference between the detection limits of our novel device and that of the conventional manual procedure. We have already developed the droplet-PCR machine, which can amplify and detect specific nucleic acids rapidly and automatically. Connecting the droplet-PCR machine to our novel automated extraction device enables PCR analysis within 15 min, and this system can be made available as a point-of-care testing in clinics as well as general hospitals.
authors.affiliation: Graduate School of Science and Technology, Shinshu University, Nagano, Japan; Core Technology Development Center, Seiko Epson Corporation, Suwa, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Yamaguchi
authors.affiliation: Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan. Electronic address: kmatsuda@shinshu-u.ac.jp.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsuda
authors.affiliation: Core Technology Development Center, Seiko Epson Corporation, Suwa, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Uehara
authors.affiliation: Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
authors.affiliation: Institute of Engineering, Academic Assembly, Shinshu University, Nagano, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Saito
mesh_terms: D001331:Automation; D057230:Limit of Detection; D008280:Magnetics; D009696:Nucleic Acids / Q000302:isolation & purification*; D012822:Silicon Dioxide
chemical_list: D009696:Nucleic Acids; D012822:Silicon Dioxide
keywords: Automated device; Magnetic silica beads; Nucleic acid extraction; Zigzag motion