title: Oral antibody to interleukin-10 reduces growth rate depression due to Eimeria spp. infection in broiler chickens.
abstract: Eimeria spp. must be controlled in floor-reared poultry to prevent the onset of coccidiosis. Here we use an oral antibody to chicken IL-10 to prevent growth depression due to Eimeria spp. infection. Egg antibody directed against an antigenic peptide of IL-10 was produced in laying hens and measured using an ELISA. In the first experiment, egg yolk powder containing antibody to chicken IL-10 (vlpramqt conjugate) (anti-IL-10 yolk powder) was fed at 3.4 g/kg feed to determine growth response following mixed Eimeria spp. challenge. Chicks were fed either anti-IL-10 antibodies or control antibodies and challenged (d3) with either sterile saline or a 10× attenuated Eimeria spp. vaccine. Control-fed and Eimeria-challenged chicks grew 8.8% slower than those challenged with saline (P < 0.04), whereas anti-IL-10-fed Eimeria challenged chicks were not different from untreated controls. In the second trial a dose response was performed with doses of either 0 (control antibody), 0.34-, or 3.4-g anti-IL-10 yolk powder/kg feed. Control-fed, Eimeria-challenged chicks grew 10.6% slower than control saline-challenged chicks (P < 0.05); however, anti-IL-10-fed chicks fed either dose of anti-IL-10 were not different from saline-challenged chicks. Finally, the effect of anti-IL-10 on acquired immunity was investigated. Chicks were fed control or anti-IL-10 yolk powder and vaccinated with a 1× dose of Eimeria vaccine at d 3. After 14 d, antibody was removed from the diet. Chicks were either saline or 10× Eimeria challenged at d 17. We found that the anti-IL-10-fed chickens did not show a reduction in growth due to challenge; hence anti-IL-10 does not appear to affect adaptive immunity during the primary immunization. Overall, use of an antibody to IL-10 is a novel method in preventing adverse effects of Eimeria spp. infection in poultry.
authors.affiliation: Animal Sciences Department, University of Wisconsin-Madison, Madison, WI.
authors.forename: Jordan M
authors.initials: JM
authors.lastname: Sand
authors.affiliation: Animal Sciences Department, University of Wisconsin-Madison, Madison, WI Comparative Biosciences Graduate Program, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI.
authors.forename: Maria K
authors.initials: MK
authors.lastname: Arendt
authors.affiliation: Animal Sciences Department, University of Wisconsin-Madison, Madison, WI.
authors.forename: Alec
authors.initials: A
authors.lastname: Repasy
authors.affiliation: Uludag University, Gorukle/Bursa, Turkey.
authors.forename: Gűlay
authors.initials: G
authors.lastname: Deniz
authors.affiliation: Animal Sciences Department, University of Wisconsin-Madison, Madison, WI mcook@wisc.edu.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Cook
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000913:Antibodies, Protozoan / Q000008:administration & dosage / Q000494:pharmacology*; D030161:Avian Proteins / Q000378:metabolism*; D002645:Chickens / Q000254:growth & development; D003048:Coccidiosis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D004032:Diet / Q000662:veterinary; D004539:Eimeria / Q000502:physiology; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism*; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*
chemical_list: D000913:Antibodies, Protozoan; D030161:Avian Proteins; D016753:Interleukin-10
keywords: Eimeria; anti-IL-10; chicken; coccidiosis; egg antibody