title: Human regulatory T cells suppress proliferation of B lymphoma cells.
abstract: Activated regulatory T cells (Tregs) suppress proliferation and differentiation of normal B cells. In our study, allogeneic polyclonal CD4 (+) CD25 (+) Tregs and CD4 (+) CD25 (+) CD127(lo)Tregs expanded in vitro in the presence of rapamycin and low dose IL-2 suppressed proliferation of 11 out of 12 established lymphoma B-cell lines. The effect of expanded CD4 (+) CD25 (+) Tregs on survival of freshly isolated lymphoma B cells maintained in culture with soluble multimeric CD40L and IL-4 was variable across lymphoma entities. The survival of freshly isolated follicular lymphoma cells usually decreased in cocultures with CD4 (+) CD25 (+) Tregs. Treg effect on chronic lymphocytic leukemia/small lymphocytic lymphoma cells ranged from suppression to help in individual patients. CD4 (+) CD25 (+) Tregs or CD4 (+) CD25 (+) CD127(lo)Tregs expanded ex vivo with rapamycin could be used to suppress regrowth of residual lymphoma after autologous hematopoietic cell transplantation (HCT), and to counteract both graft-versus-host disease and lymphoma re-growth after allogeneic HCT in select patients with lymphoma susceptible to the regulation by Tregs.
authors.affiliation: a Department of Immunology , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Monika Anna
authors.initials: MA
authors.lastname: Grygorowicz
authors.affiliation: a Department of Immunology , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Marzena
authors.initials: M
authors.lastname: Biernacka
authors.affiliation: b Department of Molecular and Translational Oncology , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Mateusz
authors.initials: M
authors.lastname: Bujko
authors.affiliation: a Department of Immunology , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Eliza
authors.initials: E
authors.lastname: Nowak
authors.affiliation: c Department of Pathology and Laboratory Diagnostics , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Rymkiewicz
authors.affiliation: d Department of Lymphoid Malignancies , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Paszkiewicz-Kozik
authors.affiliation: a Department of Immunology , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Ilona Sara
authors.initials: IS
authors.lastname: Borycka
authors.affiliation: c Department of Pathology and Laboratory Diagnostics , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Bystydzienski
authors.affiliation: d Department of Lymphoid Malignancies , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Walewski
authors.affiliation: a Department of Immunology , Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology , Warszawa , Poland ;
authors.forename: Sergiusz
authors.initials: S
authors.lastname: Markowicz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000187:drug effects / Q000473:pathology*; D044963:Biopsy, Fine-Needle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D008213:Lymphocyte Activation; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D061251:Primary Cell Culture; D020123:Sirolimus / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors; C508594:IL2 protein, human; D007376:Interleukin-2; D020123:Sirolimus
keywords: B-cell lymphoma; dendritic cells; immune regulation; regulatory T cells; tumor immunology