title: Epichlorohydrin-Cross-linked Hydroxyethyl Cellulose/Soy Protein Isolate Composite Films as Biocompatible and Biodegradable Implants for Tissue Engineering.
abstract: A series of epichlorohydrin-cross-linked hydroxyethyl cellulose/soy protein isolate composite films (EHSF) was fabricated from hydroxyethyl cellulose (HEC) and soy protein isolate (SPI) using a process involving blending, cross-linking, solution casting, and evaporation. The films were characterized with FTIR, solid-state (13)C NMR, UV-vis spectroscopy, and mechanical testing. The results indicated that cross-linking interactions occurred in the inter- and intramolecules of HEC and SPI during the fabrication process. The EHSF films exhibited homogeneous structure and relative high light transmittance, indicating there was a certain degree of miscibility between HEC and SPI. The EHSF films exhibited a relative high mechanical strength in humid state and an adjustable water uptake ratio and moisture absorption ratio. Cytocompatibility, hemocompatibility and biodegradability were evaluated by a series of in vitro and in vivo experiments. These results showed that the EHSF films had good biocompatibility, hemocompatibility, and anticoagulant effect. Furthermore, EHSF films could be degraded in vitro and in vivo, and the degradation rate could be controlled by adjusting the SPI content. Hence, EHSF films might have a great potential for use in the biomedical field.
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Yanteng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Materials Engineering, Yancheng Institute of Technology , Yancheng, Jiangsu 224051, China.
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Shiqun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gan
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Bioproducts and Bioprocesses National Science Program, Agriculture and Agri-Food Canada , 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada.
authors.forename: Peter R
authors.initials: PR
authors.lastname: Chang
authors.affiliation: Bioproducts and Bioprocesses National Science Program, Agriculture and Agri-Food Canada , 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada.
authors.forename: Debbie P
authors.initials: DP
authors.lastname: Anderson
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004811:Epichlorohydrin / Q000737:chemistry*; D005260:Female; D051379:Mice; D011817:Rabbits; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D030262:Soybean Proteins; D014867:Water; D004811:Epichlorohydrin; D002482:Cellulose; C002283:hydroxyethylcellulose
keywords: biocompatibility; biodegradability; cross-linking; hydroxyethyl cellulose; soy protein isolate