title: In situ remineralisation response of different artificial caries-like enamel lesions to home-care and professional fluoride treatments.
abstract: BACKGROUND
  Artificial lesions produced by different protocols might directly influence the response to different remineralising treatments. This study compared the response of different artificial caries-like enamel lesions to home-care and professional fluoride based-remineralising treatments in situ.
  
  
  METHODS
  The tested demineralising protocols were methylcellulose- MC gel, polyacrylic acid - PA gel, tetraethyl methylene diphosphanate - TEMDP solution, and acetate- Buffer solution. The lesions were remineralised using an in situ model, following a crossover and double blind design. Twelve subjects wore intra-oral appliances during 3 phases (3 d each): control (C) (saliva); home-care F(-) treatment (FD) (1,100 ppm F(-) dentifrice, 2x1 min/day); and professional (FVD) (22,600 ppm F(-) varnish) plus FD. The de-remineralisation was measured by transverse microradiography-TMR and hardness (surface hardness/cross-sectional hardness, SH/CSH, respectively).
  
  
  RESULTS
  For SH, lesions produced by PA gel were the only one showing significant differences among the remineralising treatments (C x FD x FVD); while the TEMDP lesion were not responsive to any fluoride treatment (for both SH/CSH). For TMR, there were no differences among the remineralising treatments, regardless of the type of lesion. Generally, the most responsive lesions to fluoride were the less demineralised lesions (considering hardness: PA gel and Buffer).
  
  
  CONCLUSIONS
  The type of lesion has influence on the surface remineralisation degree induced by home-care and professional fluoride treatments using this in situ model.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry - University of São Paulo, Al. Dr. Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil. priaranda@usp.br.
authors.forename: Priscila Maria Aranda
authors.initials: PM
authors.lastname: Salomão
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry - University of São Paulo, Al. Dr. Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil. liviacomar@yahoo.com.br.
authors.forename: Lívia Picchi
authors.initials: LP
authors.lastname: Comar
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry - University of São Paulo, Al. Dr. Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil. mbuzalaf@fob.usp.br.
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry - University of São Paulo, Al. Dr. Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil. acm@usp.br.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Magalhães
mesh_terms: D000293:Adolescent; D000328:Adult; D002327:Cariostatic Agents / Q000627:therapeutic use*; D018592:Cross-Over Studies; D003729:Dental Care; D003743:Dental Enamel / Q000473:pathology*; D003802:Dentifrices / Q000627:therapeutic use*; D004311:Double-Blind Method; D005459:Fluorides / Q000627:therapeutic use*; D006801:Humans; D009910:Oral Hygiene; D014089:Tooth Remineralization / Q000379:methods*; D055815:Young Adult
chemical_list: D002327:Cariostatic Agents; D003802:Dentifrices; D005459:Fluorides