title: Biocompatible Size-Defined Dendrimer-Albumin Binding Protein Hybrid Materials as a Versatile Platform for Biomedical Applications.
abstract: For the design of a biohybrid structure as a ligand-tailored drug delivery system (DDS), it is highly sophisticated to fabricate a DDS based on smoothly controllable conjugation steps. This article reports on the synthesis and the characterization of biohybrid conjugates based on noncovalent conjugation between a multivalent biotinylated and PEGylated poly(amido amine) (PAMAM) dendrimer and a tetrameric streptavidin-small protein binding scaffold. This protein binding scaffold (SA-ABDwt) possesses nM affinity toward human serum albumin (HSA). Thus, well-defined biohybrid structures, finalized by binding of one or two HSA molecules, are available at each conjugation step in a controlled molar ratio. Overall, these biohybrid assemblies can be used for (i) a controlled modification of dendrimers with the HSA molecules to increase their blood-circulation half-life and passive accumulation in tumor; (ii) rendering dendrimers a specific affinity to various ligands based on mutated ABD domain, thus replacing tedious dendrimer-antibody covalent coupling and purification procedures.
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Maly
authors.affiliation: Institute of Biotechnology CAS, v. v. i, Pru˚myslová 595, Vestec, ,252 42, Jesenice u Prahy, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Stanek
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Frolik
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Maly
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069, Dresden, Germany.
authors.forename: Franka
authors.initials: F
authors.lastname: Ennen
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069, Dresden, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Semeradtova
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Wrobel
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Marcel
authors.initials: M
authors.lastname: Stofik
authors.affiliation: Department of Biology, Faculty of Science, University of J.E. Purkinje, 400 96, Ústí nad Labem, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Knapova
authors.affiliation: Institute of Biotechnology CAS, v. v. i, Pru˚myslová 595, Vestec, ,252 42, Jesenice u Prahy, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Kuchar
authors.affiliation: Institute of Chemical Process Fundamentals CAS, v. v. i, Rozvojová 135, 165 02, Prague, Czech Republic.
authors.forename: Lucie Cervenkova
authors.initials: LC
authors.lastname: Stastna
authors.affiliation: Institute of Chemical Process Fundamentals CAS, v. v. i, Rozvojová 135, 165 02, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Cermak
authors.affiliation: Institute of Microbiology CAS, v. v. i, Vídeˇnská 1083, 142 20, Prague, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Sebo
authors.affiliation: Institute of Biotechnology CAS, v. v. i, Pru˚myslová 595, Vestec, ,252 42, Jesenice u Prahy, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Maly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002460:Cell Line; D050091:Dendrimers / Q000138:chemical synthesis* / Q000494:pharmacology; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011073:Polyamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D050091:Dendrimers; D008024:Ligands; C104700:PAMAM Starburst; D011073:Polyamines; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin; D011092:Polyethylene Glycols; D001710:Biotin; D019809:Streptavidin
keywords: HSA; PAMAM dendrimer; Streptavidin-biotin conjugations; binding domain proteins; biohybrid structures