title: Evaluation of a New Device for Simplifying and Standardizing Stool Sample Preparation for Viral Molecular Testing with Limited Hands-On Time.
abstract: Sensitive molecular assays have greatly improved the diagnosis of viral gastroenteritis. However, the proper preparation of stool samples for clinical testing remains an issue. bioMérieux has developed a stool preprocessing device (SPD) that includes a spoon for calibrated sampling and a vial containing buffer, glass beads, and two filters. The resulting stool filtrate is used for nucleic acid extraction. The purpose of this study was to evaluate the performance of the SPD for the quantification of human adenovirus (HAdV) DNA in stool samples collected from hematopoietic stem cell transplant (HSCT) recipients. HAdV DNA was quantified with the Adenovirus R-gene kit. The suitability of the device to reproducibly quantify HAdV DNA in stools using different extraction platforms (easyMAG and QIAsymphony) was determined using archived HAdV-positive stool samples. Coefficients of variation of HAdV DNA quantifications ranged from 1.79% to 1.83%, and no difference in quantification was observed between the two extraction systems. The HAdV DNA limit of quantification using the SPD was 3.75 log10copies/g of stool. HAdV DNA quantification using the SPD was then compared to that of the routine preprocessing technique on 75 fresh stool samples collected prospectively from pediatric HSCT recipients at risk for HAdV infections. Thirty-eight samples were HAdV DNA positive with both the SPD and routine preprocessing methods. HAdV DNA loads were on average 1.14-log10copies/g of stool higher with the SPD (P< 0.0001) than with routine methods. This new device enabled a standardized preparation of stool samples in <5 min and a reproducible and sensitive quantification of HAdV DNA. The use of the SPD for the detection of other gastrointestinal infections warrants further evaluation.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France.
authors.forename: Linda
authors.initials: L
authors.lastname: Feghoul
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France.
authors.forename: Maud
authors.initials: M
authors.lastname: Salmona
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France.
authors.forename: Janine
authors.initials: J
authors.lastname: Cherot
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Hematology Department, Hôpital Robert Debré, APHP, Paris, France.
authors.forename: Mony
authors.initials: M
authors.lastname: Fahd
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Hematology Department, Hôpital Robert Debré, APHP, Paris, France.
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Dalle
authors.affiliation: bioMérieux SA, Grenoble, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Vachon
authors.affiliation: bioMérieux SA, Grenoble, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Perrod
authors.affiliation: bioMérieux SA, Grenoble, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourgeois
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Scieux
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Hematology Department, Hôpital Robert Debré, APHP, Paris, France.
authors.forename: André
authors.initials: A
authors.lastname: Baruchel
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Simon
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France jerome.le-goff@sls.aphp.fr.
authors.forename: Jérôme
authors.initials: J
authors.lastname: LeGoff
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000821:virology; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D019562:Viral Load / Q000379:methods