title: Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars.
abstract: Incorporation of bioactive drugs and biofunctionalization of polyester fibrous scaffolds are essential means to improve their bio-functions and histocompatibility for regenerative medicine. However, it is still a challenge to biofunctionalize such drug carriers via traditional biochemical methods while maintaining their properties without changes in drug activity and loading ratio. Here, we demonstrated a facile approach for biofunctionalization of PLGA fibrous scaffolds with various molecules (i.e., PEG polymer, RGD peptide and bFGF growth factor for cell repellent, adhesion and proliferation, respectively) via mussel-Inspired poly(dopamine) (PDA) coating in aqueous solution. By virtue of the mild and efficient nature of this approach, the drug-loaded PLGA fibers could be easily biofunctionalized and showed negligible effects on the scaffold properties, especially drug activity and loading ratio. Further, in vivo study showed that, a ginsenoside-Rg3-loaded fibrous scaffold functionalized with bFGF growth factor could not only promote the early-stage wound healing in rabbit ear wounds (bio-signal from bFGF), but also inhibit later-stage hypertrophic scars formation (release of Rg3 drug). Therefore, the mussel-inspired method for bio-modification provides a facile and effective strategy to combine drug and bio-function in one system, thus facilitating a synergistic effect of drug-therapy and bio-signal when such biomaterial is used for regenerative medicine.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Ninth People's Hospital affiliated to Medical School of Shanghai Jiao Tong University, 639 Zhi Zao Ju Road, Shanghai 200011, PR China.
authors.forename: Liying
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Plastic and Reconstructive Surgery, Ninth People's Hospital affiliated to Medical School of Shanghai Jiao Tong University, 639 Zhi Zao Ju Road, Shanghai 200011, PR China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Sun
authors.affiliation: Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu, 215006, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu, 215006, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu, 215006, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Pan
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu, 215006, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu, 215006, PR China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Ninth People's Hospital affiliated to Medical School of Shanghai Jiao Tong University, 639 Zhi Zao Ju Road, Shanghai 200011, PR China. Electronic address: zhangyg18@126.com.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu, 215006, PR China. Electronic address: wgcui80@hotmail.com.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D055806:Microvessels / Q000187:drug effects / Q000473:pathology; D056951:Photoelectron Spectroscopy; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D036145:Ginsenosides; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C097367:ginsenoside Rg3; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen
keywords: Biofunctionalization; Drug-loading; Electrospun scaffold; Hypertrophic scar; Poly (dopamine)