title: Propofol suppresses invasion, angiogenesis and survival of EC-1 cells in vitro by regulation of S100A4 expression.
abstract: OBJECTIVE
  Propofol possess anticancer properties in several cancers. In the present study, we investigate the effect of propofol on the human esophageal squamous cell carcinomas (ESCC) EC-1 cells in vitro and its molecular mechanisms of action.
  
  
  METHODS
  EC-1 cells were explored to 10-100 μmol/L propofol for 72 h or 100 μmol/L/mL propofol for 24-72 h. EC-1 cells were explored to 100 μmol/L propofol for 24 h, then was transiently transfected into PcDNA3.1-S100A4 cDNA or PcDNA3.1 plasmid for 48 hrs. MTT, TUNEL, ELISA, migration, tube formation and immunoblotting were analized.
  
  
  RESULTS
  Propofol inhibits invasion, angiogenesis, proliferation and induces apoptosis in a dose and time-dependence manner, followed by deseased S100A4 expression by Western blot assay. Pre-transfection of PcDNA3.1-S100A4 cDNA inhibits propofol-induced apoptosis and promotes invasion and angiogenesis in EC-1 cells in vitro.
  
  
  CONCLUSIONS
  Propofol inhibited invasion, angiogenesis and induces apoptosis of human EC-1 cells in vitro through regulation of S100A4 expression. It not only can be an anesthesia agent, but also plays a important role of inhibiting the migration and angiogenesis of ESCC cells in the therapy of ESCC patients.
authors.affiliation: Department of Anesthesia, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangzhou, Guangdong, China. zhuangjgz@sina.com.
authors.forename: X-G
authors.initials: XG
authors.lastname: Guo
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: Y-B
authors.initials: YB
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Zhuang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004938:Esophageal Neoplasms / Q000098:blood supply / Q000188:drug therapy / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D015742:Propofol / Q000494:pharmacology*; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human; D015742:Propofol