title: Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis.
abstract: Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration.
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. kazu-azuma@muses.tottori-u.ac.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. yoshikon@shimadzu.co.jp.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Hirao
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. yo-haya@shimadzu.co.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayakawa
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. ymurahata@muses.tottori-u.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Murahata
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. tosaki@muses.tottori-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. tsuka@muses.tottori-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. imagawat@muses.tottori-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. yokamoto@muses.tottori-u.ac.jp.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. taro@muses.tottori-u.ac.jp.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Ito
keywords: Plasma-free amino acid; canine plasma amino acid levels; liquid chromatography measurements; pre- and post-food intake; pre-column labeling