title: The IL-33 gene is related to increased susceptibility to systemic sclerosis.
abstract: Systemic sclerosis (SSc) is a chronic inflammatory disease characterized by widespread fibrosis of the skin and several visceral organs. The pro-fibrotic potential of interleukin (IL)-33 has been demonstrated by in both in vitro and in vivo settings; moreover, increased level of IL-33 has also been reported in patients with SSc. Therefore, the aim of the present study was to detect the potential association of IL-33 gene polymorphisms on the susceptibility of SSc. A total of 300 SSc patients and 280 healthy controls (HC) were enrolled in this multicentric preliminary candidate gene study. DNA samples were harvested using an appropriate commercial DNA isolation kit. Four single nucleotide polymorphisms (SNPs) of IL-33 gene (rs7044343, rs1157505, rs11792633 and rs1929992) were genotyped using the appropriate commercial primer/probe sets on real-time PCR. There was no significant difference in terms of the allelic distributions and minor allele frequencies of evaluated four IL-33 polymorphisms between the SSc and HC groups (P > 0.05 for all). Moreover, the genotypic distributions of rs1157505, rs11792633 and rs1929992 polymorphisms were not significantly different (P > 0.05 for all). However, CC genotype of rs7044343 SNP was significantly higher in the SSc group compared to the HC group (P = 0.013, OR 1.75, 95 % CI 1.12-2.72). This preliminary candidate gene study demonstrates that rs7044343 polymorphism of IL-33 gene is associated with the susceptibility to the SSc in Turkish population. It may be suggested that IL-33 gene may be a candidate gene to research in SSc.
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Firat University, 23119, Elazig, Turkey. kocassk@yahoo.com.
authors.forename: Suleyman Serdar
authors.initials: SS
authors.lastname: Koca
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Uludag University, Bursa, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Pehlivan
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Sitki Kocman University, Mugla, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kara
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Marmara University, Istanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Alibaz-Oner
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Oztuzcu
authors.affiliation: Department of Rheumatology, Sisli Florence Nightingale Hospital, Istanbul, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Yilmaz
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Gozde Yildirim
authors.initials: GY
authors.lastname: Cetin
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Kisacik
authors.affiliation: Department of Rheumatology, Faculty of Medicine, 19 Mayis University, Samsun, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Ozgen
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors.forename: Omer Nuri
authors.initials: ON
authors.lastname: Pamuk
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Marmara University, Istanbul, Turkey.
authors.forename: Haner
authors.initials: H
authors.lastname: Direskeneli
authors.affiliation: Department of Rheumatology, Faculty of Medicine, 19 Mayis University, Samsun, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sayarlioglu
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Ahmet Mesut
authors.initials: AM
authors.lastname: Onat
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D000067596:Interleukin-33 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D012595:Scleroderma, Systemic / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D014421:Turkey
chemical_list: C506536:IL33 protein, human; D000067596:Interleukin-33
keywords: Genetic; IL-33 gene polymorphism; Systemic sclerosis