title: Antigen-specific T cells fully conserve antitumour function following cryopreservation.
abstract: Immunotherapies based on the autologous adoptive transfer of ex vivo-manipulated T cells are rapidly evolving for the treatment of both metastatic and primary malignancies. However, extended ex vivo culturing reduces the functionality of isolated T cells. Cryopreservation of rapidly expanded T cells for subsequent use throughout an immunotherapeutic regimen is a highly desirable recourse, thus far encumbered by a lack of studies investigating its effects on effector T-cell functionality. Here we directly compare murine tumour-reactive CD8(+) T cells cryopreserved during ex vivo expansion to freshly isolated populations. We show that cryopreservation fully conserves the differentiation potential of effector T cells, secretion of pro-inflammatory cytokines, cytotoxic function and does not impair the three-dimensional scanning motility of T cells or their capacity to infiltrate and reject tumours.
authors.affiliation: Immune Imaging Program, Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia.|Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Galeano Niño
authors.affiliation: Immune Imaging Program, Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia.|Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Rain Y Q
authors.initials: RY
authors.lastname: Kwan
authors.affiliation: Immune Imaging Program, Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia.|Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.|Department of Dermatology, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weninger
authors.affiliation: Immune Imaging Program, Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia.|Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Maté
authors.initials: M
authors.lastname: Biro
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D015925:Cryopreservation; D016207:Cytokines / Q000378:metabolism; D005240:Feasibility Studies; D006801:Humans; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*
chemical_list: D000951:Antigens, Neoplasm; D016207:Cytokines