title: Detection of Borrelia burgdorferi sensu stricto and Borrelia garinii DNAs in patient with Hyperkeratosis lenticularis perstans (Flegel disease).
abstract: Determination of the causative agent of erythema-like skin lesions in case of nonspecific superficial perivascular dermatitis was supported by histological examination and led to the latter diagnosis of Hyperkeratosis lenticularis perstans (Flegel disease) in patient. The presence of antibodies against Borrelia burgdorferi in patient serum was confirmed by a routine ELISA method and verified by Western blot technique. Skin biopsy and blood specimens were analyzed by PCR and multilocus sequence analysis (MLSA). Western blot method revealed IgG antibody response against two specific antigens, 17 and 83 kDa proteins. The recombinant test detected IgG antibody response against p100 and p41 antigens. The sequence analysis of amplicons from the selected genomic loci obtained from skin biopsy and serum samples revealed the presence of two species from B. burgdorferi sensu lato complex as a co-infection in this patient-B. burgdorferi sensu stricto (s.s.) and Borrelia garinii.
authors.affiliation: Institute of Microbiology, Faculty of Medicine, Comenius University in Bratislava and University Hospital, Bratislava, Slovakia. katarina.schwarzova@fmed.uniba.sk.
authors.forename: Katarina
authors.initials: K
authors.lastname: Schwarzova
authors.affiliation: Department of Dermatology, Faculty Hospital with Policlinic in Nove Zamky, Nove Zamky, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Kozub
authors.affiliation: Cytopatos s.r.o., Dermatopathology Unit, Bratislava, Slovakia.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Szep
authors.affiliation: Biology Center, Institute of Parasitology CAS, České Budějovice, Czech Republic.
authors.forename: Marina
authors.initials: M
authors.lastname: Golovchenko
authors.affiliation: Biology Center, Institute of Parasitology CAS, České Budějovice, Czech Republic.
authors.forename: Natasha
authors.initials: N
authors.lastname: Rudenko
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001706:Biopsy; D015153:Blotting, Western; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D060085:Coinfection / Q000382:microbiology / Q000473:pathology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007642:Keratosis / Q000382:microbiology* / Q000473:pathology; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology / Q000473:pathology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G