title: [Development and validation of an analytical method to quantify residues of cleaning products able to inactivate prion].
abstract: OBJECTIVE
  In this study, a novel analytical method to quantify prion inactivating detergent in rinsing waters coming from the washer-disinfector of a hospital sterilization unit has been developed. The final aim was to obtain an easy and functional method in a routine hospital process which does not need the cleaning product manufacturer services.
  
  
  METHODS
  An ICP-MS method based on the potassium dosage of the washer-disinfector's rinsing waters was developed. Potassium hydroxide is present on the composition of the three prion inactivating detergent currently on the French market. The detergent used in this study was the Actanios LDI(®) (Anios laboratories). A Passing and Bablok regression compares concentrations measured with this developed method and with the HPLC-UV manufacturer method.
  
  
  RESULTS
  According to results obtained, the developed method is easy to use in a routine hospital process. The Passing and Bablok regression showed that there is no statistical difference between the two analytical methods during the second rinsing step. Besides, both methods were linear on the third rinsing step, with a 1.5ppm difference between the concentrations measured for each method.
  
  
  CONCLUSIONS
  This study shows that the ICP-MS method developed is nonspecific for the detergent, but specific for the potassium element which is present in all prion inactivating detergent currently on the French market. This method should be functional for all the prion inactivating detergent containing potassium, if the sensibility of the method is sufficient when the potassium concentration is very low in the prion inactivating detergent formulation.
authors.affiliation: Service de stérilisation, centre hospitalier universitaire d'Angers, 4, rue Larrey, 49933 Angers, France; Micro- et nanomédecines biomimétiques, Inserm U1066, université Nantes Angers Le Mans, 4, rue Larrey, 49933 Angers, France.
authors.forename: T
authors.initials: T
authors.lastname: Briot
authors.affiliation: Service de stérilisation, centre hospitalier universitaire d'Angers, 4, rue Larrey, 49933 Angers, France.
authors.forename: A
authors.initials: A
authors.lastname: Robelet
authors.affiliation: Laboratoire de pharmacologie-toxicologie, centre hospitalier universitaire d'Angers, 4, rue Larrey, 49933 Angers, France.
authors.forename: N
authors.initials: N
authors.lastname: Morin
authors.affiliation: Micro- et nanomédecines biomimétiques, Inserm U1066, université Nantes Angers Le Mans, 4, rue Larrey, 49933 Angers, France.
authors.forename: J
authors.initials: J
authors.lastname: Riou
authors.affiliation: Laboratoire de pharmacologie-toxicologie, centre hospitalier universitaire d'Angers, 4, rue Larrey, 49933 Angers, France; Groupe d'étude des interactions hôtes-pathogènes, UPRES EA 3142, université Nantes Angers Le Mans, 4, rue Larrey, 49933 Angers, France.
authors.forename: B
authors.initials: B
authors.lastname: Lelièvre
authors.affiliation: Service de stérilisation, centre hospitalier universitaire d'Angers, 4, rue Larrey, 49933 Angers, France. Electronic address: avlebelle-dehaut@chu-angers.fr.
authors.forename: A-V
authors.initials: AV
authors.lastname: Lebelle-Dehaut
mesh_terms: D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection; D004350:Drug Residues / Q000494:pharmacology; D006761:Hospitals; D013058:Mass Spectrometry; D011188:Potassium / Q000032:analysis; D011328:Prions / Q000187:drug effects*; D015203:Reproducibility of Results; D012915:Soaps / Q000494:pharmacology*; D013242:Sterilization
chemical_list: D004202:Disinfectants; D011328:Prions; D012915:Soaps; D011188:Potassium
keywords: ICP-MS; Laveur désinfecteur; Potassium; Prion; Sterilization; Stérilisation; Washer-disinfector