title: Impact of the chemicals, essential for the purification process of strict Fe-hydrogenase, on the corrosion of mild steel.
abstract: The influence of additional chemical molecules, necessary for the purification process of [Fe]-hydrogenase from Clostridium acetobutylicum, was studied on the anaerobic corrosion of mild steel. At the end of the purification process, the pure [Fe-Fe]-hydrogenase was recovered in a Tris-HCl medium containing three other chemicals at low concentration: DTT, dithionite and desthiobiotin. Firstly, mild steel coupons were exposed in parallel to a 0.1 M pH7 Tris-HCl medium with or without pure hydrogenase. The results showed that hydrogenase and the additional molecules were in competition, and the electrochemical response could not be attributed solely to hydrogenase. Then, solutions with additional chemicals of different compositions were studied electrochemically. DTT polluted the electrochemical signal by increasing the Eoc by 35 mV 24 h after the injection of 300 μL of control solutions with DTT, whereas it drastically decreased the corrosion rate by increasing the charge transfer resistance (Rct 10 times the initial value). Thus, DTT was shown to have a strong antagonistic effect on corrosion and was removed from the purification process. An optimal composition of the medium was selected (0.5 mM dithionite, 7.5 mM desthiobiotin) that simultaneously allowed a high activity of hydrogenase and a lower impact on the electrochemical response for corrosion tests.
authors.affiliation: Laboratoire de Génie chimique, CNRS-Université Fédérale de Toulouse (INP, UPS), 4 allée Emile Monso, BP 84234, 31432 Toulouse, France.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Rouvre
authors.affiliation: Université Fédérale de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, F-60200 Compiègne, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Gauquelin
authors.affiliation: Université Fédérale de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, F-60200 Compiègne, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Meynial-Salles
authors.affiliation: Laboratoire de Génie chimique, CNRS-Université Fédérale de Toulouse (INP, UPS), 4 allée Emile Monso, BP 84234, 31432 Toulouse, France. Electronic address: regine.basseguy@ensiacet.fr.
authors.forename: Régine
authors.initials: R
authors.lastname: Basseguy
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism*; D003343:Corrosion; D004227:Dithionite / Q000378:metabolism*; D004229:Dithiothreitol / Q000378:metabolism*; D055664:Electrochemical Techniques; D004867:Equipment Design; D006864:Hydrogenase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013232:Steel / Q000737:chemistry*
chemical_list: D007506:Iron-Sulfur Proteins; D013232:Steel; D004227:Dithionite; D001710:Biotin; C004749:desthiobiotin; C118830:iron hydrogenase; D006864:Hydrogenase; D004229:Dithiothreitol
keywords: Anaerobic corrosion; Desthiobiotin; Dithionite; Dithiothreitol (DTT); Hydrogenase; Mild steel