title: Facile fabrication of poly(L-lactic acid) microsphere-incorporated calcium alginate/hydroxyapatite porous scaffolds based on Pickering emulsion templates.
abstract: In this study, we develop a facile one-pot approach to the fabrication of poly(L-lactic acid) (PLLA) microsphere-incorporated calcium alginate (ALG-Ca)/hydroxyapatite (HAp) porous scaffolds based on HAp nanoparticle-stabilized oil-in-water Pickering emulsion templates, which contain alginate in the aqueous phase and PLLA in the oil phase. The emulsion aqueous phase is solidified by in situ gelation of alginate with Ca(2+) released from HAp by decreasing pH with slow hydrolysis of D-gluconic acid δ-lactone (GDL) to produce emulsion droplet-incorporated gels, followed by freeze-drying to form porous scaffolds containing microspheres. The pore structure of porous scaffolds can be adjusted by varying the HAp or GDL concentration. The compressive tests show that the increase of HAp or GDL concentration is beneficial to improve the compressive property of porous scaffolds, while the excessive HAp can lead to the decrease in compressive property. Moreover, the swelling behavior studies display that the swelling ratios of porous scaffolds reduce with increasing HAp or GDL concentration. Furthermore, hydrophobic drug ibuprofen (IBU) and hydrophilic drug bovine serum albumin (BSA) are loaded into the microspheres and scaffold matrix, respectively. In vitro drug release results indicate that BSA has a rapid release while IBU has a sustained release in the dual drug-loaded scaffolds. In vitro cell culture experiments verify that mouse bone mesenchymal stem cells can proliferate on the porous scaffolds well, indicating the good biocompatibility of porous scaffolds. All these results demonstrate that the PLLA microsphere-incorporated ALG-Ca/HAp porous scaffolds have a promising potential for tissue engineering and drug delivery applications.
authors.affiliation: Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China; Institute of Biomaterials, College of Materials and Energy, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Ma
authors.affiliation: Institute of Biomaterials, College of Materials and Energy, South China Agriculture University, Guangzhou 510642, China. Electronic address: yangzhuohong@yahoo.com.cn.
authors.forename: Zhuohong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Biomaterials, College of Materials and Energy, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Wuyi
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China.
authors.forename: Zhengshan
authors.initials: Z
authors.lastname: Du
authors.affiliation: Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yi
authors.affiliation: Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China. Electronic address: zhywang@scut.edu.cn.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Wang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000493:pharmacokinetics; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D065546:Drug Liberation; D017886:Durapatite / Q000737:chemistry*; D004655:Emulsions; D005942:Gluconates / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007052:Ibuprofen / Q000737:chemistry / Q000493:pharmacokinetics; D019344:Lactic Acid / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004655:Emulsions; D005942:Gluconates; D006603:Hexuronic Acids; D007783:Lactones; D011091:Polyesters; D011108:Polymers; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide); D020723:Glucuronic Acid; D017886:Durapatite; C106477:glucono-1,4-lactone; D007052:Ibuprofen
keywords: Alginate; Hydroxyapatite; Microspheres; Nanocomposite porous scaffolds; Pickering emulsion; Poly(L-lactic acid)