title: Novel glucometer-based immunosensing strategy suitable for complex systems with signal amplification using surfactant-responsive cargo release from glucose-encapsulated liposome nanocarriers.
abstract: Methods based on surfactant-responsive controlled release systems of cargoes from nanocontainers have been developed for bioanalytical applications, but most were utilized for drug delivery and a few reports were focused on immunoassays. Herein we design an in situ amplified immunoassay protocol for high-efficient detection of aflatoxins (aflatoxin B1, AFB1 used in this case) based on surfactant-responsive cargo release from glucose-encapsulated liposome nanocarriers with sensitivity enhancement. Initially, biotinylated liposome nanocarrier encapsulated with glucose was synthesized using a reverse-phase evaporation method. Thereafter, the nanocarrier was utilized as the signal-generation tag on capture antibody-coating microplate through classical biotin-avidin linkage after reaction with biotinylated detection antibody. Upon addition of buffered surfactant (1X PBS-Tween 20 buffer) into the medium, the surfactant immediately hydrolyzed the conjugated liposome, and released the encapsulated glucose from the nanocarriers, which could be quantitatively determined by using a low-cost personal glucometer (PGM). The detectable signal increased with the increment of target analyte. Under the optimal conditions, the assay could allow PGM detection toward target AFB1 as low as 0.6 pg mL(-1) (0.6 ppt). Moreover, the methodology also showed good reproducibility and high specificity toward target AFB1 against other mycotoxins and proteins, and was applicable for quantitatively monitoring target AFB1 in the complex systems, e.g., naturally contaminated/spiked peanut samples and serum specimens, with the acceptable results. Taking these advantages of simplification, low cost, universality and sensitivity, our design provides a new horizon for development of advanced immunoassays in future point-of-care testing.
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China. Electronic address: juantang@jxnu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Yapei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Huiqiong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Cengceng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China. Electronic address: dianping.tang@fzu.edu.cn.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000097:blood*; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D010367:Arachis / Q000737:chemistry / Q000382:microbiology; D001231:Aspergillus flavus / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D001710:Biotin / Q000737:chemistry; D015190:Blood Glucose Self-Monitoring; D002417:Cattle; D003692:Delayed-Action Preparations / Q000737:chemistry; D005947:Glucose / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008081:Liposomes / Q000737:chemistry*; D011817:Rabbits; D015203:Reproducibility of Results; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D003692:Delayed-Action Preparations; D008081:Liposomes; D013501:Surface-Active Agents; D001710:Biotin; D016604:Aflatoxin B1; D005947:Glucose
keywords: Aflatoxin B(1); Glucose-encapsulated liposome nanocarriers; Immunoassay; Personal glucometer; Surfactant-responsive cargo release