title: Exposure to fipronil elevates systolic blood pressure and disturbs related biomarkers in plasma of rats.
abstract: Recent reports show that fipronil affects non-target organisms, including environmental species populations and potentially humans. We aimed to examine if fipronil exposure affects the systolic blood pressure and related biomarkers. Thus, fipronil was orally administered to rats (30 mg/kg/day) during 15 days (Fipronil group) or physiological solution (Control group). While fipronil increased significantly the systolic blood pressure (158±13 mmHg), no significant changes were observed in Control group (127±3 mmHg). Significantly, higher levels of fipronil in plasma were observed in Fipronil group (0.46±0.09 μg/mL versus 0.17±0.11 μg/mL in Control group). Fipronil group showed lower weight gain compared with Control group. While fipronil resulted in higher concentrations of endothelin-1, reduced antioxidant capacity and lower levels of circulating matrix metalloproteinase 2 (MMP-2) and nitric oxide (NO) metabolites compared to Control group, no alteration was observed in serum biomarkers of renal and hepatic/biliary functional abilities. Therefore, this study suggests that fipronil causes hypertension and endothelin-1 plays a key role. Also, these findings suggest that reductions of both MMP-2 and NO may contribute with the elevation of systolic blood pressure observed with fipronil.
authors.affiliation: Center for Toxicological Assistance, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil.
authors.forename: Joao Leandro
authors.initials: JL
authors.lastname: Chaguri
authors.affiliation: Center for Toxicological Assistance, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil.
authors.forename: Antonio Francisco
authors.initials: AF
authors.lastname: Godinho
authors.affiliation: Center for Toxicological Assistance, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil.
authors.forename: Daniel França
authors.initials: DF
authors.lastname: Horta
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil.
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Gonçalves-Rizzi
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil.
authors.forename: Jose Sergio
authors.initials: JS
authors.lastname: Possomato-Vieira
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil.
authors.forename: Regina Aparecida
authors.initials: RA
authors.lastname: Nascimento
authors.affiliation: Center for Toxicological Assistance, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil; Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. Electronic address: carlosjunior@ibb.unesp.br.
authors.forename: Carlos Alan
authors.initials: CA
authors.lastname: Dias-Junior
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001794:Blood Pressure / Q000187:drug effects*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D011720:Pyrazoles / Q000633:toxicity*; D051381:Rats
chemical_list: D015415:Biomarkers; D007306:Insecticides; D011720:Pyrazoles; D009569:Nitric Oxide; D020778:Matrix Metalloproteinase 2; C082360:fipronil
keywords: Biomarkers; Cardiovascular toxicity; Fipronil; Pesticides