title: Asymmetric Mach-Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection.
abstract: In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si₃N₄ Asymmetric Mach-Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab'). We measured a best volumetric sensitivity of 10⁴ rad/RIU, leading to a Limit of Detection below 5 × 10(-7) RIU. On sensors functionalized with Fab', we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.
authors.affiliation: Nanoscience Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123 Povo (TN), Italy. tatevik.chalyan@unitn.it.
authors.forename: Tatevik
authors.initials: T
authors.lastname: Chalyan
authors.affiliation: Nanoscience Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123 Povo (TN), Italy. romain.guider@unitn.it.
authors.forename: Romain
authors.initials: R
authors.lastname: Guider
authors.affiliation: LaBSSAH, Fondazione Bruno Kessler, Via Sommarive 18, 38123 Povo, Italy. pasqua@fbk.eu.
authors.forename: Laura
authors.initials: L
authors.lastname: Pasquardini
authors.affiliation: LaBSSAH, Fondazione Bruno Kessler, Via Sommarive 18, 38123 Povo, Italy. mzanetti@fbk.eu.
authors.forename: Manuela
authors.initials: M
authors.lastname: Zanetti
authors.affiliation: LioniX B.V., Enschede 7522, The Netherlands. f.h.falke@lionixbv.nl.
authors.forename: Floris
authors.initials: F
authors.lastname: Falke
authors.affiliation: LioniX B.V., Enschede 7522, The Netherlands. f.schreuder@lionixbv.nl.
authors.forename: Erik
authors.initials: E
authors.lastname: Schreuder
authors.affiliation: LioniX B.V., Enschede 7522, The Netherlands. r.g.heideman@lionixbv.nl.
authors.forename: Rene G
authors.initials: RG
authors.lastname: Heideman
authors.affiliation: LaBSSAH, Fondazione Bruno Kessler, Via Sommarive 18, 38123 Povo, Italy. pederzo@fbk.eu.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pederzolli
authors.affiliation: Nanoscience Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123 Povo (TN), Italy. lorenzo.pavesi@unitn.it.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Pavesi
mesh_terms: D016607:Aflatoxin M1 / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D007368:Interferometry; D055620:Optical Phenomena; D017785:Photons; D015203:Reproducibility of Results; D017655:Silicon Compounds / Q000737:chemistry*
chemical_list: D017655:Silicon Compounds; D016607:Aflatoxin M1; C032734:silicon nitride
keywords: Aflatoxin M1; Fab′; Mach–Zehnder interferometer; biosensor; limit of detection