title: [Interaction of Flightless I with Nup88 and Importin β].
abstract: High expression of Fightless I (FLII) is associated to multiple tumors. Based on our previous study that FLII might be involved in the nuclear export, we assessed the possible interaction of FLII with the nuclear envelop associating proteins Importin β and Nup88. We first constructed GST-FLII, GST-LRR recombinant plasmids and transformed them into the Rosetta strain to produce GST-FLII, GST-LRR fusion protein. After purification of these proteins, GST-pull down, as well as co-immunoprecipitation, were used to test the interaction of FLII with Importin β and Nup88. FLII interacted with Importin β and Nup88, and FLII LRR domain is responsible for these interactions. Thus, FLII may play a role in nuclear export through interaction with Importin β and Nup88.
authors.forename: Shengyou
authors.initials: S
authors.lastname: Liao
authors.forename: Cuihua
authors.initials: C
authors.lastname: Wang
authors.forename: Dong'e
authors.initials: D
authors.lastname: Tang
authors.forename: Jinmei
authors.initials: J
authors.lastname: Wei
authors.forename: Yujiao
authors.initials: Y
authors.lastname: He
authors.forename: Haiting
authors.initials: H
authors.lastname: Xiong
authors.forename: Fengmei
authors.initials: F
authors.lastname: Xu
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Gao
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liu
authors.forename: Langxia
authors.initials: L
authors.lastname: Liu
mesh_terms: D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015534:Trans-Activators; D028961:beta Karyopherins / Q000378:metabolism*
chemical_list: C098179:FLII protein, human; D008840:Microfilament Proteins; C104835:NUP88 protein, human; D028861:Nuclear Pore Complex Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D028961:beta Karyopherins