title: Hepatic Transcriptome Responses of Domesticated and Wild Turkey Embryos to Aflatoxin B₁.
abstract: The mycotoxin, aflatoxin B₁ (AFB₁) is a hepatotoxic, immunotoxic, and mutagenic contaminant of food and animal feeds. In poultry, AFB₁ can be maternally transferred to embryonated eggs, affecting development, viability and performance after hatch. Domesticated turkeys (Meleagris gallopavo) are especially sensitive to aflatoxicosis, while Eastern wild turkeys (M. g. silvestris) are likely more resistant. In ovo exposure provided a controlled AFB₁ challenge and comparison of domesticated and wild turkeys. Gene expression responses to AFB₁ in the embryonic hepatic transcriptome were examined using RNA-sequencing (RNA-seq). Eggs were injected with AFB₁ (1 μg) or sham control and dissected for liver tissue after 1 day or 5 days of exposure. Libraries from domesticated turkey (n = 24) and wild turkey (n = 15) produced 89.2 Gb of sequence. Approximately 670 M reads were mapped to a turkey gene set. Differential expression analysis identified 1535 significant genes with |log₂ fold change| ≥ 1.0 in at least one pair-wise comparison. AFB₁ effects were dependent on exposure time and turkey type, occurred more rapidly in domesticated turkeys, and led to notable up-regulation in cell cycle regulators, NRF2-mediated response genes and coagulation factors. Further investigation of NRF2-response genes may identify targets to improve poultry resistance.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA. monso181@umn.edu.
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Monson
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA. ccardona@umn.edu.
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Cardona
authors.affiliation: Department of Animal, Dairy and Veterinary Sciences, College of Agriculture, Utah State University, Logan, UT 84322, USA. roger@usu.edu.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Coulombe
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA. reedx054@umn.edu.
authors.forename: Kent M
authors.initials: KM
authors.lastname: Reed
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D008297:Male; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D059467:Transcriptome / Q000187:drug effects*; D014422:Turkeys / Q000235:genetics*
chemical_list: D016604:Aflatoxin B1
keywords: RNA-seq; aflatoxin; differential expression; domesticated turkey; embryonic; liver; wild turkey