title: Pan_02 murine pancreatic cancer model.
abstract: OBJECTIVE
  The aim of this study was to investigate and describe basic features of Pan_02 murine pancreatic ade- nocarcinoma tumor model. Pan_02 has very low sensitivity to chemotherapeutics therefore it is very similar to human pancreatic cancers.
  
  
  METHODS
  Mice were subcutaneously injected with different number of cells and tumor growth was measured. Tumors were also investigated with ultrasonograph VEVO2100 in doppler mode to detect viable and function- al blood vessels. Collected tumor samples were investigated to asses necrosis and blood vessel permeability.
  
  
  RESULTS
  We found substantial differences in tumor growth depending on a number of inoculated tumor cells. Mice injected with 0.5 × 106 cells gave the most consistent pattern of growth. All tumors showed substantial vascularisation but bigger tumors were more likely to develop larger blood vessels instead of a more dense network.
  
  
  CONCLUSIONS
  Murine Pan_02 cancer model shares many features with human PDAC cancers and therefore it is a good model to study new drugs. Injection of 0.5 × 106 cells gives consistent results although it requires more time for the tumor to grow. It also allows the immune system to adapt. Owing ta good vascularisation, Pan_02 is a good model to study chemotherapy against pancreatic adenocarcinoma but it may not be the best model for immunotherapy since it does not respond to the immune stimulation (unpublished data).
authors.affiliation: Department of Immunology, Jagiellonian University Medical College, Czysta 18, Krakow, Poland. krzykawski.marcin@gmail.com.
authors.forename: Marcin Przemysław
authors.initials: MP
authors.lastname: Krzykawski
authors.forename: Martyna
authors.initials: M
authors.lastname: Krzykawska-Serda
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jasińska
authors.forename: Janusz
authors.initials: J
authors.lastname: Marcinkiewicz
mesh_terms: D000818:Animals; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured