title: Supersonic Shear Wave Elastography of Response to Anti-cancer Therapy in a Xenograft Tumor Model.
abstract: Our objective was to determine if supersonic shear wave elastography (SSWE) can detect changes in stiffness of a breast cancer model under therapy. A human invasive carcinoma was implanted in 22 mice. Eleven were treated with an anti-angiogenic therapy and 11 with glucose for 24 d. Tumor volume and stiffness were assessed during 2 wk before treatment and 0, 7, 12, 20 and 24 d after the start of therapy using SSWE. Pathology was assessed after 12 and 24 d of treatment. We found that response to therapy was associated with early softening of treated tumors only, resulting in a significant difference from non-treated tumors after 12 d of treatment (p = 0.03). On pathology, large areas of necrosis were observed at 12 d in treated tumors. Although treatment was still effective, treated tumors subsequently stiffened during a second phase of the treatment (days 12-24), with a small amount of necrosis observed on pathology on day 24. In conclusion, SSWE was able to measure changes in the stiffness of tumors in response to anti-cancer treatment. However, stiffness changes associated with good response to treatment may change over time, and increased stiffness may also reflect therapy efficacy.
authors.affiliation: Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, UMR-S970, Paris, France; Radiology Department, Université Paris Descartes Sorbonne Paris Cité, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France. Electronic address: foucauld.chammings@egp.aphp.fr.
authors.forename: Foucauld
authors.initials: F
authors.lastname: Chamming's
authors.affiliation: Pathology Department, Université Paris Descartes Sorbonne Paris Cité, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Marie-Aude
authors.initials: MA
authors.lastname: Le-Frère-Belda
authors.affiliation: Institut Langevin-ondes et images, Ecole Supérieure de Physique et de Chimie Industrielle (ESPCI), Paris, France.
authors.forename: Heldmuth
authors.initials: H
authors.lastname: Latorre-Ossa
authors.affiliation: Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, UMR-S970, Paris, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Fitoussi
authors.affiliation: Radiology Department, Université Paris Descartes Sorbonne Paris Cité, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Alban
authors.initials: A
authors.lastname: Redheuil
authors.affiliation: U612 Institut National de la Sante et de la Recherche Medicale, Pharmacologie Pre-clinique Antitumorale, Paris, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Assayag
authors.affiliation: Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, UMR-S970, Paris, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Pidial
authors.affiliation: Institut Langevin-ondes et images, Ecole Supérieure de Physique et de Chimie Industrielle (ESPCI), Paris, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Gennisson
authors.affiliation: Institut Langevin-ondes et images, Ecole Supérieure de Physique et de Chimie Industrielle (ESPCI), Paris, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Tanter
authors.affiliation: Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, UMR-S970, Paris, France; Radiology Department, Université Paris Descartes Sorbonne Paris Cité, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Charles-André
authors.initials: CA
authors.lastname: Cuénod
authors.affiliation: Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, UMR-S970, Paris, France; Radiology Department, Université Paris Descartes Sorbonne Paris Cité, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Laure S
authors.initials: LS
authors.lastname: Fournier
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology*; D045744:Cell Line, Tumor; D016903:Drug Monitoring / Q000379:methods*; D055119:Elastic Modulus / Q000187:drug effects; D054459:Elasticity Imaging Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents
keywords: Breast cancer; Pathology; Response to therapy; Shear wave elastography; Stiffness changes; Ultrasound