title: Superoxide dismutases in chronic gastritis.
abstract: Human gastric diseases have shown significant changes in the activity and expression of superoxide dismutase (SOD) isoforms. The aim of this study was to detect Mn-SOD activity and expression in the tissue of gastric mucosa, primarily in chronic gastritis (immunohistochemical Helicobacter pylori-negative gastritis, without other pathohistological changes) and to evaluate their possible connection with pathohistological diagnosis. We examined 51 consecutive outpatients undergoing endoscopy for upper gastrointestinal symptoms. Patients were classified based on their histopathological examinations and divided into three groups: 51 patients (archive samples between 2004-2009) with chronic immunohistochemical Helicobacter pylori-negative gastritis (mononuclear cells infiltration were graded as absent, moderate, severe) divided into three groups. Severity of gastritis was graded according to the updated Sydney system. Gastric tissue samples were used to determine the expression of Mn-SOD with anti-Mn-SOD Ab immunohistochemically. The Mn-SOD expression was more frequently present in specimens with severe and moderate inflammation of gastric mucosa than in those with normal mucosa. In patients with normal histological finding, positive immunoreactivity of Mn-SOD was not found. Our results determine the changes in Mn-SOD expression occurring in the normal gastric mucosa that had undergone changes in the intensity of chronic inflammatory infiltrates in the lamina propria.
authors.affiliation: Department of Pathology and Cytology, General Hospital Vinkovci, Vinkovci, Croatia.|Department of Pathology, Faculty of Medicine, University of Osijek, Osijek, Croatia.
authors.forename: Dražen
authors.initials: D
authors.lastname: Švagelj
authors.affiliation: Department of Urology, General Hospital Vinkovci, Vinkovci, Croatia.
authors.forename: Velimir
authors.initials: V
authors.lastname: Terzić
authors.affiliation: Department of Clinical and Laboratory Diagnostics, Faculty of Medicine, University of Osijek, Osijek, Croatia.
authors.forename: Jasna
authors.initials: J
authors.lastname: Dovhanj
authors.affiliation: Department of Surgery, General Hospital Vinkovci, Vinkovci, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Švagelj
authors.affiliation: Department of Pathology and Cytology, General Hospital Vinkovci, Vinkovci, Croatia.
authors.forename: Mirta
authors.initials: M
authors.lastname: Cvrković
authors.affiliation: Department of Pathology and Cytology, General Hospital Vinkovci, Vinkovci, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Švagelj
mesh_terms: D000906:Antibodies / Q000737:chemistry; D016022:Case-Control Studies; D002908:Chronic Disease; D005753:Gastric Mucosa / Q000201:enzymology* / Q000473:pathology; D005756:Gastritis / Q000175:diagnosis* / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D005773:Gastroscopy; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D010045:Outpatients; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000906:Antibodies; D013482:Superoxide Dismutase
keywords: Mn-SOD; chronic inflammation; gastric mucosa