title: Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes.
abstract: BACKGROUND
  Preeclampsia is associated with inadequate invasion of trophocytes and spiral artery remodeling. As a β-D-glucuronidase enzyme, Heparanase is related to tumor angiogenesis, development and invasion. Trophocytes have similar characteristics to tumor cells, and heparanase could therefore play an important role in the pathogenesis of preeclampsia.
  
  
  METHODS
  The expression of heparanase in severe preeclampsia and normal placentas was detected via real-time PCR, immunohistochemistry and western blotting. The effects of heparanase on trophocytes migration and invasion were investigated by culturing the HTR-8/Svneo cell line with recombinant human heparanase protein in vitro.
  
  
  RESULTS
  The levels of inactive 65-kDa heterologous heparanase dimers were obviously increased, and the content of the 50-kDa active polypeptide was decreased in severe preeclampsia. Furthermore, exogenous heparanase protein could reduce the migration and invasion of HTR-8/Svneo cells.
  
  
  CONCLUSIONS
  Our results suggested that heparanase might be an important factor in the pathogenesis of severe preeclampsia.
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
authors.forename: Yan-Yun
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Luo
keywords: Preeclampsia; heparanase; invasion; trophocyte