title: Pseudomonas aeruginosa and Heterotrophic Bacteria Count in Bottled Waters in Iran.
abstract: BACKGROUND
  Nowadays, due to increased public awareness about water pollution and water borne diseases as well as water network deficiencies, bottled water consumers have increased dramatically worldwide, including Iran. Pseudomonas aeruginosa is an opportunistic human pathogen capable of causing widespread infections in burn and immune-compromised patients. The aim of this study was to investigate, P. aeruginosa in bottled waters selling in Iranian markets.
  
  
  METHODS
  One hundred and twenty samples of five unknown (not famous) domestic bottled water brands were purchased from Tehran retailers during 2013. The samples were evaluated for the presence of P. aeruginosa. In addition, heterotrophic plate counts were determined by incubation at 37 °C for 24 h.
  
  
  RESULTS
  P. aeruginosa was detected in 36.7% (44 samples) of all samples examined. In addition, heterotrophic bacteria in 32.5% (39 samples) of the samples were higher than 100 CFU/mL, while in 7.5% (9 samples) of the samples HPC relied between 20 and 100 CFU/ml.
  
  
  CONCLUSIONS
  In contrast to public believe, bottled waters are not free of microorganisms, and it is suggested that authorities should provide stricter monitoring and control plan for water resources and plants. Concerning HPC and P. aeruginosa brands B and D were not suitable for drinking.
authors.affiliation: Dept. of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Matin
authors.initials: M
authors.lastname: Mohammadi Kouchesfahani
authors.affiliation: Dept. of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Alimohammadi
authors.affiliation: Dept. of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Nabizadeh Nodehi
authors.affiliation: Dept. of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Aslani
authors.affiliation: Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sassan
authors.initials: S
authors.lastname: Rezaie
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Samieh
authors.initials: S
authors.lastname: Asadian
keywords: Bottled water; Heterotrophic plate count; Pseudomonas aeruginosa