title: Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma.
abstract: Oral squamous cell carcinoma (OSCC) has been reported as the most prevalent cancer of the head and neck region, while early diagnosis remains challenging. Here we took a comprehensive bioinformatics study on microarray data of 326 OSCC clinical samples with control of 165 normal tissues. The cell interaction pathways of ECM-receptor interaction and focal adhesion were found to be significantly regulated in OSCC samples. Further analysis of the topological properties and expression consistency identified that three hub genes in the gene interaction network, MMP9, PDIA3 and BGH3, were consistently up-expressed in OSCC samples. When being validated on additional microarray datasets of 41 OSCC samples, the validation rate of over-expressed BGH3, MMP9, and PDIA3 reached 90%, 90% and 84% respectively. At last, immuno-histochemical assays were done to test the protein expression of the three genes on newly collected clinical samples of 35 OSCC, 20 samples of pre-OSCC stage, and 12 normal oral mucosa specimens. Their protein expression levels were also found to progressively increase from normal mucosa to pre-OSCC stage and further to OSCC (ANOVA p = 0.000), suggesting their key roles in OSCC pathogenesis. Based on above solid validation, we propose BGH3, MMP9 and PDIA3 might be further explored as potential biomarkers to aid OSCC diagnosis.
authors.affiliation: Department of Oral Medicine, School of Stomatology, Tongji University, Shanghai, 200092, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Oral Medicine, School of Stomatology, Tongji University, Shanghai, 200092, China.
authors.forename: Fangyang
authors.initials: F
authors.lastname: Shao
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Pi
authors.affiliation: Department of Oral Medicine, School of Stomatology, Tongji University, Shanghai, 200092, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Shi
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Oral Medicine, School of Stomatology, Tongji University, Shanghai, 200092, China.
authors.forename: Diping
authors.initials: D
authors.lastname: Gong
authors.affiliation: Department of Oral Medicine, School of Stomatology, Tongji University, Shanghai, 200092, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Advanced Institute of Translational Medicine, Tongji University, Shanghai, 200092, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Tang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D030541:Databases, Genetic; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D000099222:betaIG-H3 Protein
chemical_list: D014408:Biomarkers, Tumor; D016326:Extracellular Matrix Proteins; D020780:Matrix Metalloproteinase 9; D019704:Protein Disulfide-Isomerases; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C085868:PDIA3 protein, human