title: Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone.
abstract: The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.
authors.affiliation: Department of Chemistry and Chemical Biology, School of Science and Technology, Gunma University.
authors.forename: Kiichi
authors.initials: K
authors.lastname: Sato
authors.forename: Sayaka
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Eri
authors.initials: E
authors.lastname: Yoshida
authors.forename: Reina
authors.initials: R
authors.lastname: Ishii
authors.forename: Naoki
authors.initials: N
authors.lastname: Sasaki
authors.forename: Kin-ichi
authors.initials: K
authors.lastname: Tsunoda
authors.forename: Kae
authors.initials: K
authors.lastname: Sato
mesh_terms: D000818:Animals; D001577:Benzophenones / Q000737:chemistry*; D002448:Cell Adhesion / Q000528:radiation effects; D002450:Cell Communication / Q000528:radiation effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004867:Equipment Design; D006801:Humans; D020014:K562 Cells; D056656:Lab-On-A-Chip Devices; D008253:Macaca mulatta; D008856:Microscopy, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D001577:Benzophenones; D003432:Cross-Linking Reagents; C047723:benzophenone