title: The effect of Flos Lonicerae Japonicae extract on gastro-intestinal motility function.
abstract: BACKGROUND
  Flos Lonicerae Japonicae is a well-known herb of traditional Chinese medicine that has been used for heat-clearing, detoxification, anti-inflammation, throat pain and gastro-intestinal (GI) disorder. In order to verify the effect of Flos Lonicerae Japonicae on GI disorder, we investigated the prokinetic effect of GC-7101 on GI motility function.
  
  
  METHODS
  GC-7101 is the standardized extract of Flos Lonicerae Japonicae. The contractile action of GC-7101 on feline esophageal smooth muscle cell (ESMC) was evaluated by measuring dispersed cell length. The isometric tension study was performed to investigate the effect of GC-7101 on feline lower esophageal sphincther (LES). The prokinetic effect of GC-7101 was investigated by gastric emptying (GE) and gastro-intestinal transit (GIT) in rats.
  
  
  RESULTS
  GC-7101 produced concentration-dependent contractions in ESMCs. Pretreatment with 5-HT3 and 5-HT4 receptor blocker (ondansetron and GR113808) inhibited the contractile responses of the GC-7101-induced ESMCs. In isometric tension study, GC-7101 recovered the HCl-induced decreased tone of LES muscle strips. The treatment of GC-7101 enhanced the carbachol-induced contractile responses and the electric field stimulation (EFS)-induced on-contraction. The oral administration of GC-7101 not only significantly accelerated GE and GIT in normal rats but also recovered the delayed GE and GIT, and its effect was more potent than that of conventional prokinetics (e.g., domperidone, a dopamine-receptor antagonist, and mosapride, a 5-HT4-receptor agonist).
  
  
  CONCLUSIONS
  GC-7101 revealed a prokinetic effect through enhancing the contractile responses of ESMCs, tone increases, enhancing the carbarchol- or EFS-induced contractile responses of LES muscle strips, and the acceleration of GE and GIT. We have identified the significant potential of GC-7101 for the development of new prokinetic drugs through this study.
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-784, Republic of Korea. Electronic address: yoonnamjin@naver.com.
authors.forename: Yoonjin
authors.initials: Y
authors.lastname: Nam
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-784, Republic of Korea. Electronic address: J45505@naver.com.
authors.forename: Jong Mi
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-784, Republic of Korea. Electronic address: widelia_324@hotmail.com.
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-784, Republic of Korea. Electronic address: laia0906@naver.com.
authors.forename: Hyun Su
authors.initials: HS
authors.lastname: Ha
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-784, Republic of Korea. Electronic address: udsohn@cau.ac.kr.
authors.forename: Uy Dong
authors.initials: UD
authors.lastname: Sohn
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002415:Cats; D002478:Cells, Cultured; D004294:Domperidone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D049630:Esophageal Sphincter, Lower / Q000187:drug effects; D005746:Gastric Emptying / Q000187:drug effects; D005769:Gastrointestinal Motility / Q000187:drug effects*; D005772:Gastrointestinal Transit / Q000187:drug effects; D041743:Herb-Drug Interactions; D007211:Indoles / Q000494:pharmacology; D031239:Lonicera; D008297:Male; D009025:Morpholines / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D017294:Ondansetron / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D001549:Benzamides; D004365:Drugs, Chinese Herbal; C000612343:GC-7101; D007211:Indoles; D009025:Morpholines; D010936:Plant Extracts; D013449:Sulfonamides; D017294:Ondansetron; D004294:Domperidone; D002217:Carbachol; C062720:mosapride; C082871:GR 113808
keywords: Atropine (PubChem CID: 174174); Carbachol (PubChem CID: 5831); Cisplatin (PubChem CID: 2767); Esophageal smooth muscle cells; GR113808 (PubChem CID: 119376); Gastric emptying; Gastro-intestinal system; Gastrointestinal transit; L-NNA (PubChem CID: 440005); Lonicerae Japonicae Thunberg; Loperamide hydrochloride (PubChem CID: 71420); Lower esophageal sphincter; Ondansetron hydrochloride dehydrate (PubChem CID: 68647)