title: Luliconazole Retention in Stratum Corneum and Prevention of Fungal Infection in a Guinea Pig Tinea Pedis Model.
abstract: OBJECTIVE
  To compare drug concentrations in the stratum corneum following daily application of luliconazole and terbinafine cream in a guinea pig tinea pedis model.
  
  
  METHODS
  Luliconazole 1% cream or terbinafine 1% cream were topically applied once daily to hind limbs of guinea pigs for 14 days. Drug concentration in stratum corneum of plantar skin was measured by HPLC-UV on days 1, 3, 7, 10, and 14. Separately, creams were applied daily for 5 days to the hind limbs of guinea pigs and skin drug release determined. In addition, drug retention in the stratum corneum was assessed by infecting guinea pigs with Trichophyton mentagrophytes, 14 and 21 days after a single application of luliconazole or terbinafine creams.
  
  
  RESULTS
  Luliconazole stratum corneum concentrations were higher than those of terbinafine throughout the study. Concentrations of luliconazole and terbinafine were 71.6μg/g and 36.6μg/g, respectively, after a single application (P<.05), reaching steady state after 10 days. Cumulative release of luliconazole from the stratum corneum was 4.5 times greater than with terbinafine. Unlike terbinafine, no fungal invasion of the stratum corneum was seen 14 days post-treatment with luliconazole.
  
  
  CONCLUSIONS
  Drug concentrations of luliconazole in the stratum corneum and subsequent release are greater than those achieved with terbinafine and may contribute to clinical efficacy. Luliconazole may also provide greater protection against disease recurrence.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Koga
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Nanjoh
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Toga
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Pillai
authors.forename: William
authors.initials: W
authors.lastname: Jo
authors.forename: Ryoji
authors.initials: R
authors.lastname: Tsuboi
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004817:Epidermis / Q000378:metabolism*; D006168:Guinea Pigs; D007093:Imidazoles / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008297:Male; D014008:Tinea Pedis / Q000517:prevention & control*
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; C112528:luliconazole