title: Transducin-like enhancer of split-1 is expressed and functional in human macrophages.
abstract: Macrophages display heterogeneous phenotypes, including the classical M1 proinflammatory and the alternative M2 anti-inflammatory polarization states. The transducin-like enhancer of split-1 (TLE1) is a transcriptional corepressor whose functions in macrophages have not been studied yet. We report that TLE1 is highly expressed in human alternative macrophages in vitro and in atherosclerotic plaques as well as in adipose tissue M1/M2 mixed macrophages. TLE1 silencing in alternative macrophages decreases the expression of the M2 markers IL-1Ra and IL-10, while it exacerbates TNFα and CCL3 induction by lipopolysaccharide. Hence, TLE1 is expressed in human macrophages where it has potential anti-inflammatory and alternative phenotype promoting properties.
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Federica
authors.initials: F
authors.lastname: De Paoli
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Copin
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Vanhoutte
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Derudas
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Manjula
authors.initials: M
authors.lastname: Vinod
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Zawadzki
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Susen
authors.affiliation: Inserm, CHU Lille, University of Lille, U1190, EGID, France.
authors.forename: François
authors.initials: F
authors.lastname: Pattou
authors.affiliation: CHU Lille, France.
authors.forename: Stéphan
authors.initials: S
authors.lastname: Haulon
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Bart
authors.initials: B
authors.lastname: Staels
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
authors.forename: Jérome
authors.initials: J
authors.lastname: Eeckhoute
authors.affiliation: Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.|INSERM, U 1081, Institute for Research on Cancer and Aging of Nice (IRCAN), 'Aging and Diabetes' team, University of Nice-Sophia Antipolis, France.|Clinical Chemistry Laboratory, University Hospital, Nice, France.
authors.forename: Giulia
authors.initials: G
authors.lastname: Chinetti-Gbaguidi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015992:Body Mass Index; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D056970:Co-Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D050152:Intra-Abdominal Fat / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D057507:Mice, 129 Strain; D009765:Obesity / Q000097:blood / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D058226:Plaque, Atherosclerotic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D056970:Co-Repressor Proteins; C508609:IL10 protein, human; C507763:IL1RN protein, human; C508598:IL4 protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D011994:Recombinant Proteins; D012097:Repressor Proteins; C499043:TLE1 protein, human; C434946:Tle1 protein, mouse; D016753:Interleukin-10; D015847:Interleukin-4
keywords: atherosclerosis; inflammation; macrophage polarization; transcription factors