title: Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samples.
abstract: The quantification of human genomic DNA is a necessary first step in the DNA casework sample analysis workflow. DNA quantification determines optimal sample input amounts for subsequent STR (short tandem repeat) genotyping procedures, as well as being a useful screening tool to identify samples most likely to provide probative genotypic evidence. To better mesh with the capabilities of newest-generation STR analysis assays, the Quantifiler(®) HP and Quantifiler(®) Trio DNA Quantification Kits were designed for greater detection sensitivity and more robust performance with samples that contain PCR inhibitors or degraded DNA. The new DNA quantification kits use multiplex TaqMan(®) assay-based fluorescent probe technology to simultaneously quantify up to three human genomic targets, allowing samples to be assessed for total human DNA, male contributor (i.e., Y-chromosome) DNA, as well as a determination of DNA degradation state. The Quantifiler HP and Trio Kits use multiple-copy loci to allow for significantly improved sensitivity compared to earlier-generation kits that employ single-copy target loci. The kits' improved performance provides better predictive ability for results with downstream, newest-generation STR assays, and their shortened time-to-result allows more efficient integration into the forensic casework analysis workflow.
authors.affiliation: Thermo Fisher Scientific, Human Identification Group, 180 Oyster Point Blvd., South San Francisco, CA 94080, United States.
authors.forename: Allison
authors.initials: A
authors.lastname: Holt
authors.affiliation: Thermo Fisher Scientific, Human Identification Group, 180 Oyster Point Blvd., South San Francisco, CA 94080, United States.
authors.forename: Sharon Chao
authors.initials: SC
authors.lastname: Wootton
authors.affiliation: Thermo Fisher Scientific, Human Identification Group, 180 Oyster Point Blvd., South San Francisco, CA 94080, United States.
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Mulero
authors.affiliation: Thermo Fisher Scientific, Human Identification Group, 180 Oyster Point Blvd., South San Francisco, CA 94080, United States.
authors.forename: Pius M
authors.initials: PM
authors.lastname: Brzoska
authors.affiliation: Thermo Fisher Scientific, Human Identification Group, 180 Oyster Point Blvd., South San Francisco, CA 94080, United States.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Langit
authors.affiliation: Thermo Fisher Scientific, Human Identification Group, 180 Oyster Point Blvd., South San Francisco, CA 94080, United States. Electronic address: bob.green@thermofisher.com.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Green
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000097:blood / Q000235:genetics; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D053803:Forensic Genetics / Q000295:instrumentation / Q000379:methods; D060005:Genotyping Techniques / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA
keywords: DNA quantification; Forensic science; Quantitative real-time PCR