title: Selective elimination of the free fatty acid fraction from esterified fatty acids in rat plasma through chemical derivatization and immobilization on amino functionalized silica nano-particles.
abstract: A high throughput and low cost approach to separate free fatty acids (FFAs) from phospholipid and acylglycerols (esterified fatty acids, EFAs) has been demonstrated, which may be widely used as a sample preparation method in the metabolomics and lipid research. The optimal conditions for FFAs reacting with N-hydroxysuccinimide (NHS) only need 10min at room temperature to obtain a 93.5% yield of FFAs-NHS ester. The rest 6% FFA transformed into N-cyclohexyl-fatty acid-amide which is stable to methyl esterification adopted for fatty acids analysis. 10min are taken for FFAs-NHS ester to react with amino functionalized silica nanoparticles to immobilize the FFAs. The separation of FFAs from EFAs could be carried out readily by centrifugation. The whole process including derivatization, immobilization, and centrifugation takes less than 40min. Much more accurate fatty acids composition of rat plasma EFAs could be obtained by this approach than the previous reported methods.
authors.affiliation: Beijing Institute of Microchemistry, No.15 Xinjiangongmen Road, Haidian District, Beijing 100091, China. Electronic address: chenjun05@tsinghua.org.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Beijing Institute of Microchemistry, No.15 Xinjiangongmen Road, Haidian District, Beijing 100091, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lyu
authors.affiliation: Functional Nanomaterials Laboratory, Center for Micro/Nanomaterials and Technology and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry (TIPC), Chinese Academy of Sciences, No.29 Zhongguancun East Road, Haidian District, Beijing 100190, China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Yang
authors.affiliation: Beijing Institute of Microchemistry, No.15 Xinjiangongmen Road, Haidian District, Beijing 100091, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Functional Nanomaterials Laboratory, Center for Micro/Nanomaterials and Technology and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry (TIPC), Chinese Academy of Sciences, No.29 Zhongguancun East Road, Haidian District, Beijing 100190, China. Electronic address: jhhe@mail.ipc.ac.cn.
authors.forename: Junhui
authors.initials: J
authors.lastname: He
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification*; D005230:Fatty Acids, Nonesterified / Q000737:chemistry / Q000302:isolation & purification*; D005989:Glycerides / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry
chemical_list: D004952:Esters; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D010743:Phospholipids; D013388:Succinimides; D012822:Silicon Dioxide; C001426:N-hydroxysuccinimide
keywords: Amino functionalized silica nanoparticles; Esterified fatty acids; Free fatty acids; Lipids; Sample preparation