title: Effects of chromium-enriched Bacillus subtilis KT260179 supplementation on growth performance, caecal microbiology, tissue chromium level, insulin receptor expression and plasma biochemical profile of mice under heat stress.
abstract: The aim of this study was to investigate the effect of providing supplementary Cr-enriched Bacillus subtilis (CEBS) to mice with regard to their growth performance, caecal microbiology, tissue Cr concentration, insulin receptor (IR) expression and plasma biochemical profile. A total of ninety-six Kunming strain mice were allocated to four different groups: control, CEBS, inorganic Cr and B. subtilis. After 15 d of treatment, mice that received CEBS or normal B. subtilis had higher body weights than control mice, and after 30 d mice given either CEBS or B. subtilis had greater body weights than control mice or those given inorganic Cr. The concentration of Cr in tissues (heart, liver, spleen, kidney and skeletal muscle) increased after CEBS supplementation. B. subtilis and CEBS supplementation caused a significant increase in the numbers of Lactobacillus and Bifidobacterium in the caecum, whereas the numbers of Escherichia coli and Staphylococcus decreased significantly compared with the control. The levels of IR RNA and protein in skeletal muscles increased significantly. Plasma glucose, total cholesterol, TAG and LDL-cholesterol levels declined significantly in the CEBS group compared with the control group, whereas plasma insulin and HDL-cholesterol levels increased significantly. In conclusion, CEBS supplementation enhanced the regulation of body growth, increased tissue organic Cr concentrations, altered caecal microbiota and enhanced IR expression to produce significant changes in plasma biochemistry.
authors.affiliation: The Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,no. 40 Nongke South Road,Hefei 230031,Anhui,People's Republic of China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Yang
authors.affiliation: The Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,no. 40 Nongke South Road,Hefei 230031,Anhui,People's Republic of China.
authors.forename: Yayuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: The Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,no. 40 Nongke South Road,Hefei 230031,Anhui,People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: The Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,no. 40 Nongke South Road,Hefei 230031,Anhui,People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,no. 40 Nongke South Road,Hefei 230031,Anhui,People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: The Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,no. 40 Nongke South Road,Hefei 230031,Anhui,People's Republic of China.
authors.forename: Chonglong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D001412:Bacillus subtilis; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002432:Cecum / Q000187:drug effects / Q000382:microbiology*; D002784:Cholesterol / Q000097:blood; D002857:Chromium / Q000378:metabolism / Q000494:pharmacology*; D000069196:Gastrointestinal Microbiome; D006358:Hot Temperature; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D012333:RNA, Messenger; D014280:Triglycerides; D002857:Chromium; D002784:Cholesterol; D011972:Receptor, Insulin
keywords: ADG average daily gain; CEBS Cr-enriched Bacillus subtilis; Cr-enriched Bacillus; F:G ratio of feed:gain; GAPDH glyceraldehyde phosphate dehydrogenase; Gene expression; Heat stress; IR insulin receptor; Metabolism; Mice; TC total cholesterol