title: Identification of activation-tagArabidopsismutants with altered production of germination stimulants forPhelipanche ramosa(L.).
abstract: Germination of seeds of root parasites like broomrapes (Orobanchaceae) is tightly regulated by chemical products exuded from the roots of the host plant, known as germination stimulants (GSs). Changes in the levels of synthesis and emission of GS can allow the development of practical measures for control of the crops-harming parasitic species. However, the genes encoding enzymes responsible for GS biosynthesis are still unknown. We performed a large-scale screening of 62,000Arabidopsisactivation-tag mutants for alteration in susceptibility toPhelipanche ramosaand to identify lines with altered GS production among them. After five successive screenings we identified 36 lines with altered susceptibility toP. ramosa. Seven of them displayed altered levels of GS production. By using a combination of Southern blot and thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR), we pinpointed the location of activation-tag constructs in these lines. A combination of differential display and quantitative real-time PCR (qRT-PCR) allowed us to identify several affected genes. Two of them are directly involved in isoprenoid biosynthetic pathway in chloroplasts, and we believe that their activation led to increased levels of GS production. We believe that these genes are responsible for increased GS production in five of theArabidopsislines resistant toP. ramosa.
authors.affiliation: Department of Plant Physiology and Molecular Biology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Ina
authors.initials: I
authors.lastname: Kirilova
authors.affiliation: Department of Plant Physiology and Molecular Biology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Iliya D
authors.initials: ID
authors.lastname: Denev
authors.affiliation: Department of Plant Physiology and Molecular Biology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Rumyana
authors.initials: R
authors.lastname: Bineva
authors.affiliation: Department of Plant Physiology and Molecular Biology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Maria
authors.initials: M
authors.lastname: Gevezova
authors.affiliation: Agricultural Academy, AgroBioInstitute , Sofia , Bulgaria.
authors.forename: Milena
authors.initials: M
authors.lastname: Alexandrova
authors.affiliation: Agricultural Academy, AgroBioInstitute , Sofia , Bulgaria.
authors.forename: Kaloyan
authors.initials: K
authors.lastname: Kostov
authors.affiliation: Agricultural Academy, AgroBioInstitute , Sofia , Bulgaria.
authors.forename: Rossitza
authors.initials: R
authors.lastname: Batchvarova
keywords: Arabidopsis; Phelipanche ramosa; activation-tag mutants; broomrapes; germination stimulants