title: A single 60-min bout of peristaltic pulse external pneumatic compression transiently upregulates phosphorylated ribosomal protein s6.
abstract: We investigated whether a single 60-min bout of whole leg, peristaltic pulse external pneumatic compression (EPC) altered select growth factor-related mRNAs and/or various phospho(p)-proteins related to cell growth, proliferation, inflammation and apoptosis signalling (e.g. Akt-mTOR, Jak-Stat). Ten participants (8 males, 2 females; aged 22·2 ± 0·4 years) reported to the laboratory 4 h post-prandial, and vastus lateralis muscle biopsies were obtained prior to (PRE), 1 h and 4 h post-EPC treatment. mRNA expression was analysed using real-time RT-PCR and phosphophorylated and cleaved proteins were analysed using an antibody array. No changes in selected growth factor-related mRNAs were observed following EPC. All p-proteins significantly altered by EPC decreased, except for p-rps6 (Ser235/236) which increased 31% 1 h post-EPC compared to PRE levels (P = 0·016). Notable decreases also included p-BAD (Ser112; -28%, P = 0·004) at 4 h post-EPC compared to PRE levels. In summary, an acute bout of EPC transiently upregulates p-rps6 as well as affecting other markers in the Akt-mTOR signalling cascade. Future research should characterize whether chronic EPC application promotes alterations in lower-limb musculature and/or enhances exercise-induced training adaptations.
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.|School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: J S
authors.identifier: http://orcid.org/0000-0001-6762-086X
authors.initials: JS
authors.lastname: Martin
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Kephart
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Mobley
authors.affiliation: The Orthopaedic Clinic, Auburn, AL, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Wilson
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.|Athletics Department, Auburn University, Auburn, AL, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Goodlett
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.|School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Roberts
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D048228:Intermittent Pneumatic Compression Devices; D008297:Male; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D052097:Quadriceps Muscle / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D038601:Ribosomal Protein S6 / Q000378:metabolism*; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D038601:Ribosomal Protein S6; C546842:MTOR protein, human; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases
keywords: Akt-mTOR signalling; external pneumatic compression; phosphorylation; rps6; skeletal muscle