title: Cell free fetal DNA in the plasma of pregnant women with preeclampsia.
abstract: OBJECTIVE
  Insufficient cytotrophoblast invasion to the myometrium is associated with preeclampsia, especially with the early-onset preeclampsia (before 34 gestational weeks). Several investigations have marked changes in the concentration of cell free fetal DNA in the maternal circulation of women with preeclampsia. However, these studies were not performed for early or late preeclampsia subgroups individually. The present authors planned to determine the levels of the cell free both fetal and maternal DNA in the maternal circulation in early preeclampsia subgroup and compare it with normotensive control cohort.
  
  
  METHODS
  A total of 16 women; eight of these with preeclampsia and eight normotensive control cohorts with singleton male pregnancy between 28 and 32 gestational weeks were included in the study. Real-time PCR analysis was performed for determining the circulating cell free DNA levels.
  
  
  RESULTS
  Cell free fetal DNA concentrations were higher in early preeclamptic women than control subjects. The authors found no statistically significant difference in each levels of maternal and total DNA between hypertensive and normotensive groups.
  
  
  CONCLUSIONS
  The present findings suggest that the levels of cell free fetal DNA in maternal circulation were higher in pregnancies which are complicated with early preeclampsia than normotensive controls.
authors.forename: M M
authors.initials: MM
authors.lastname: Seval
authors.forename: H G
authors.initials: HG
authors.lastname: Karabulut
authors.forename: A
authors.initials: A
authors.lastname: Tükün
authors.forename: A
authors.initials: A
authors.lastname: Koç
mesh_terms: D000328:Adult; D001794:Blood Pressure; D016022:Case-Control Studies; D004247:DNA / Q000097:blood*; D017931:DNA Primers; D005260:Female; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D008297:Male; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D017931:DNA Primers; D004247:DNA