title: Molecular Basis for the Interaction Between AP4 β4 and its Accessory Protein, Tepsin.
abstract: The adaptor protein 4 (AP4) complex (ϵ/β4/μ4/σ4 subunits) forms a non-clathrin coat on vesicles departing the trans-Golgi network. AP4 biology remains poorly understood, in stark contrast to the wealth of molecular data available for the related clathrin adaptors AP1 and AP2. AP4 is important for human health because mutations in any AP4 subunit cause severe neurological problems, including intellectual disability and progressive spastic para- or tetraplegias. We have used a range of structural, biochemical and biophysical approaches to determine the molecular basis for how the AP4 β4 C-terminal appendage domain interacts with tepsin, the only known AP4 accessory protein. We show that tepsin harbors a hydrophobic sequence, LFxG[M/L]x[L/V], in its unstructured C-terminus, which binds directly and specifically to the C-terminal β4 appendage domain. Using nuclear magnetic resonance chemical shift mapping, we define the binding site on the β4 appendage by identifying residues on the surface whose signals are perturbed upon titration with tepsin. Point mutations in either the tepsin LFxG[M/L]x[L/V] sequence or in its cognate binding site on β4 abolish in vitro binding. In cells, the same point mutations greatly reduce the amount of tepsin that interacts with AP4. However, they do not abolish the binding between tepsin and AP4 completely, suggesting the existence of additional interaction sites between AP4 and tepsin. These data provide one of the first detailed mechanistic glimpses at AP4 coat assembly and should provide an entry point for probing the role of AP4-coated vesicles in cell biology, and especially in neuronal function.
authors.affiliation: Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA.|Center for Structural Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Meredith N
authors.initials: MN
authors.lastname: Frazier
authors.affiliation: Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, UK.
authors.forename: Alexandra K
authors.initials: AK
authors.lastname: Davies
authors.affiliation: Center for Structural Biology, Vanderbilt University, Nashville, TN, USA.|Department of Biochemistry and Chemistry, Vanderbilt University, Nashville, TN, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Voehler
authors.affiliation: Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA.|Center for Structural Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Amy K
authors.initials: AK
authors.lastname: Kendall
authors.affiliation: Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Georg H H
authors.initials: GH
authors.lastname: Borner
authors.affiliation: Center for Structural Biology, Vanderbilt University, Nashville, TN, USA.|Department of Biochemistry and Chemistry, Vanderbilt University, Nashville, TN, USA.
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Chazin
authors.affiliation: Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, UK.
authors.forename: Margaret S
authors.initials: MS
authors.lastname: Robinson
authors.affiliation: Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA.|Center for Structural Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Lauren P
authors.initials: LP
authors.lastname: Jackson
mesh_terms: D033964:Adaptor Protein Complex 4 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D017354:Point Mutation; D011485:Protein Binding
chemical_list: D033964:Adaptor Protein Complex 4
keywords: adaptor protein complexes; biochemistry; cell biology; membrane trafficking; non-clathrin coats; structural biology; vesicle coats