title: Propagation of field highly pathogenic porcine reproductive and respiratory syndrome virus in MARC-145 cells is promoted by cell apoptosis.
abstract: Infection of porcine reproductive and respiratory syndrome virus (PRRSV) induces cell apoptosis both in vivo and in vitro. However, the correlation between host cell apoptosis and PRRSV replication is unclear. Here, the promotion of PRRSV propagation by cell apoptosis in MARC-145 cells was reported. The observation on propagation of field highly pathogenic PRRSV (HP-PRRSV) in MARC-145 cells showed that infection of overgrown MARC-145 cells obviously elevated virus production and cell apoptosis was triggered in these cells before virus inoculation. The investigation on propagation of field HP-PRRSV in apoptosis induced MARC-145 cells displayed that induction of apoptosis further increased the virus production and a vigorous viral RNA replication accompanied by fast virus release in these cells was detected in the initial 24h post infection. In addition, when field HP-PRRSV was serially passed in drug-treated MARC-145 cells, the progeny viruses kept a stable viral titer and infectivity to its native target cells in the tested generations. In summary, these findings demonstrated that apoptotic MARC-145 cells were more susceptible to field HP-PRRSV and propagation of the virus was promoted by effective replication and cell-to-cell transmission of the virus in these cells.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Mengyun
authors.initials: M
authors.lastname: Ge
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China. Electronic address: zengfanya@scu.edu.cn.
authors.forename: Fanya
authors.initials: F
authors.lastname: Zeng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000254:growth & development*; D014779:Virus Replication
keywords: Apoptosis; Cell-to-cell transmission; Field HP-PRRSV; Infectious cell cluster; MARC-145 cells