title: Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice.
abstract: OBJECTIVE
  An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.
  
  
  RESULTS
  Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group.
  
  
  CONCLUSIONS
  Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
authors.forename: Stella Siaw Xiu
authors.initials: SS
authors.lastname: Joan
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Jee
authors.initials: J
authors.lastname: Pui-Fong
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
authors.forename: Adelene Ai-Lian
authors.initials: AA
authors.lastname: Song
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Li-Yen
authors.initials: LY
authors.lastname: Chang
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.|Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Sazaly
authors.initials: S
authors.lastname: AbuBakar
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia. raha@upm.edu.my.|Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia. raha@upm.edu.my.
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013294:Lactococcus lactis / Q000235:genetics / Q000254:growth & development*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013381:Subtilisins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000914:Antibodies, Viral; D001426:Bacterial Proteins; C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007071:Immunoglobulin A, Secretory; D007252:Influenza Vaccines; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C080937:NisP protein, Lactococcus lactis; D013381:Subtilisins
keywords: H1N1; Haemagglutinin; Influenza virus; Lactococcus lactis; Oral vaccine; Surface display; Vaccine