title: Functional Analysis of the Glucuronyltransferases GlcAT-P and GlcAT-S of Drosophila melanogaster: Distinct Activities towards the O-linked T-antigen.
abstract: The Drosophila melanogaster glucuronyltransferases dGlcAT-S and dGlcAT-P were reported to be expressed ubiquitously and results of in vitro activity assays indicate a functional redundancy. We analyzed both transferases in vivo and in vitro and could show significant differences in their activity towards N-and O-glycoproteins in vivo. While GlcAT-P is able to use N-linked N-acetyllactosamine chains and the O-linked T-antigen as a substrate to form non-sulfated HNK1- (GlcAβ1-3Galβ1-4GlcNAcβ1-) and glucuronyl-T-antigens in vivo, GlcAT-S adds glucuronic acid only to N-linked chains, thereby synthesizing only the non-sulfated HNK1-antigen.
authors.affiliation: Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany. isabelle.breloy@uni-koeln.de.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Breloy
authors.affiliation: Octapharma Biopharmaceuticals GmbH, Im Neuenheimer Feld 590, Heidelberg 69220, Germany. tilo.schwientek@octapharma.com.
authors.forename: Tilo
authors.initials: T
authors.lastname: Schwientek
authors.affiliation: Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany. althoffd@smail.uni-koeln.de.
authors.forename: Deborah
authors.initials: D
authors.lastname: Althoff
authors.affiliation: Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany. marvin.holz@hhu.de.
authors.forename: Marvin
authors.initials: M
authors.lastname: Holz
authors.affiliation: Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany. TimKoppen@web.de.
authors.forename: Tim
authors.initials: T
authors.lastname: Koppen
authors.affiliation: Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany. akrupa@gmx.de.
authors.forename: Angelika
authors.initials: A
authors.lastname: Krupa
authors.affiliation: Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany. franz.hanisch@uni-koeln.de.
authors.forename: Franz-Georg
authors.initials: FG
authors.lastname: Hanisch
mesh_terms: D000606:Amino Sugars / Q000378:metabolism; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D020723:Glucuronic Acid / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques
chemical_list: D000606:Amino Sugars; D000957:Antigens, Viral, Tumor; D029721:Drosophila Proteins; C000458:N-acetyllactosamine; D020723:Glucuronic Acid; C473770:GlcAT-P protein, Drosophila; C473769:GlcAT-S protein, Drosophila; D014453:Glucuronosyltransferase
keywords: Drosophila melanogaster; N-glycans; O-glycans; glucuronyltransferases; glycomics; mass spectrometry