title: Screening and Characterization of Lactic Acid Bacteria Strains with Anti-inflammatory Activities through in vitro and Caenorhabditis elegans Model Testing.
abstract: The present study was conducted to screen candidate probiotic strains for anti-inflammatory activity. Initially, a nitric oxide (NO) assay was used to test selected candidate probiotic strains for anti-inflammatory activity in cultures of the murine macrophage cell line, RAW 264.7. Then, the in vitro probiotic properties of the strains, including bile tolerance, acid resistance, and growth in skim milk media, were investigated. We also performed an in vitro hydrophobicity test and an intestinal adhesion assay using Caenorhabditis elegans as a surrogate in vivo model. From our screening, we obtained 4 probiotic candidate lactic acid bacteria (LAB) strains based on their anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell cultures and the results of the in vitro and in vivo probiotic property assessments. Molecular characterization using 16S rDNA sequencing analysis identified the 4 LAB strains as Lactobacillus plantarum. The selected L. plantarum strains (CAU1054, CAU1055, CAU1064, and CAU1106) were found to possess desirable in vitro and in vivo probiotic properties, and these strains are good candidates for further investigations in animal models and human clinical studies to elucidate the mechanisms underlying their anti-inflammatory activities.
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Hye Kyoung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Sun-Hae
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Cho Rong
authors.initials: CR
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Mi Ri
authors.initials: MR
authors.lastname: Park
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam 463-746, Korea.
authors.forename: Myung-Ki
authors.initials: MK
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Geun-Bae
authors.initials: GB
authors.lastname: Kim
keywords: Caenorhabditis elegans; anti-inflammatory; lactic acid bacteria; probiotics