title: Phosphorylation of 4EBP by oral leucine administration was suppressed in the skeletal muscle of PGC-1α knockout mice.
abstract: Leucine is known to increase mTOR-mediated phosphorylation of 4EBP. In this study, leucine was administered to skeletal muscle-PGC-1α knockout mice. We observed attenuated 4EBP phosphorylation in the skeletal muscle, but not in the liver, of the PGC-1α knockout mice. These data suggest that skeletal muscle-PGC-1α is important for leucine-mediated mTOR activation and protein biosynthesis.
authors.affiliation: a Graduate School of Life and Environmental Sciences , Kyoto Prefectural University , Kyoto , Japan.|b Research Fellow of Japan Society for the Promotion of Science , Tokyo , Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Yoshimura
authors.affiliation: a Graduate School of Life and Environmental Sciences , Kyoto Prefectural University , Kyoto , Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Minami
authors.affiliation: c Laboratory of Animal Models for Human Diseases , National Institutes of Biomedical Innovation, Health and Nutrition , Osaka , Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Matsuda
authors.affiliation: d Section of Cardiology, Department of Medicine , University of Chicago , Chicago , IL , USA.|e Global COE Program , Tokyo Medical and Dental University , Tokyo , Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sawada
authors.affiliation: f Graduate School of Nutritional and Environmental Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miura
authors.affiliation: a Graduate School of Life and Environmental Sciences , Kyoto Prefectural University , Kyoto , Japan.
authors.forename: Yasutomi
authors.initials: Y
authors.lastname: Kamei
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000284:Administration, Oral; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins; D039642:Eukaryotic Initiation Factors; D005786:Gene Expression Regulation; D007930:Leucine / Q000008:administration & dosage*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009928:Organ Specificity; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C499527:Eif4ebp1 protein, mouse; D039642:Eukaryotic Initiation Factors; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010750:Phosphoproteins; C485290:Ppargc1a protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; C546843:mTOR protein, mouse; D058570:TOR Serine-Threonine Kinases; D007930:Leucine
keywords: 4EBP; leucine; mTOR; muscle specific PGC-1α knockout mouse