title: Topoisomerase-Based Preparation and AFM Imaging of Multi-Interlocked Circular DNA.
abstract: Multi-interlocked circular DNA structures have been in high demand for fabricating complicated functional DNA architectures and nanodevices such as molecular switches, shuttles, and motors. Even though various innovative methods have been developed in the past, creation of multi-interlocked circular DNA structures with defined numbers of DNA molecules and linking patterns is still a challenging task nowadays. Here, we propose a top-down decatenation of kinetoplast DNA as a new approach for creating multi-interlocked circular DNA structures. Through optimizing the amount and reaction time of topoisomerase II, we synthesized completely mutually interlocked tricircular, tetra-circular, and oligo-circular DNA structures, which have not yet been acquirable through any other existing synthetic means. The catenation structures of multiple circular DNA were further verified through atomic force microscopic analysis of the backbone overlapping patterns and the circumference. It accordingly is our expectation that the top-down enzymatic approaches could offer a highly interlocked network with defined numbers of circular DNA with simple protocols, and could consequently be beneficial to the design and fabrication of sophisticated functional molecules and nanodevices in the areas of supramolecular chemistry, DNA nanotechnology, and material science.
authors.affiliation: Lexington High School , 251 Waltham Street, Lexington, Massachusetts 02421, United States.
authors.forename: Tevin
authors.initials: T
authors.lastname: Li
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore, 637371.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore, 637371.
authors.forename: Lianzhe
authors.initials: L
authors.lastname: Hu
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore, 637371.
authors.forename: Fangwei
authors.initials: F
authors.lastname: Shao
mesh_terms: D004250:DNA Topoisomerases, Type II / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry*; D018625:Microscopy, Atomic Force
chemical_list: D004270:DNA, Circular; D004250:DNA Topoisomerases, Type II