title: Analyses of C-Reactive Protein, Endothelial Nitric Oxide Synthase and Interleukin-6 Gene Polymorphisms in Adolescents with a Family History of Premature Coronary Artery Disease: A Pilot Study.
abstract: BACKGROUND
  Family history of premature atherosclerosis imposes a high risk to people. The relationship between atherosclerosis and gene polymorphisms of various biomarkers such as Endothelial Nitric Oxide Synthase (eNOS), C-Reactive Protein (CRP), and Interleukin-6 (IL-6) has shown in previous studies.
  
  
  OBJECTIVE
  The major aim of the study was to evaluate the CRP, eNOS, and IL-6 gene polymorphisms in a group of adolescents who have a parental history of early coronary artery disease (CAD).
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  Thirty-six volunteers with a father with obstructive CAD during the first four decades and 46 subjects with a father with normal coronary arteries documented with coronary angiography were included in the study. Polymerase chain reaction-restriction fragment length polymorphism techniques were used to analyze CRP, eNOS, and IL-6 polymorphisms.
  
  
  RESULTS
  We did not find any differences between the two groups with regard to age, sex, body mass index, renal functions, systolic and diastolic blood pressures, lipid profile, and fasting glucose, hemoglobin, and high sensitivity CRP. A significant difference was only observed in IL-6-572 G/C genotype distribution and allele frequency between two groups (Pc=0.036 OR=3.48 CI (95%) 1.17-10.32).
  
  
  CONCLUSIONS
  The present study showed a significant association between the IL-6-572 G/C gene polymorphism (presence of C allele) and adolescents with a parental history of premature CAD.
authors.affiliation: Department of Cardiology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Ataç
authors.initials: A
authors.lastname: Çelik
authors.affiliation: Department of Pediactics, Süleymaniye Obstetrics and Gynecology Training and Research Hospital, İstanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Özçetin
authors.affiliation: Department of Medical Biology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Ateş
authors.affiliation: Department of Cardiology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Altunkaş
authors.affiliation: Department of Cardiology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Kayıhan
authors.initials: K
authors.lastname: Karaman
authors.affiliation: Department of Cardiovascular Surgery, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: İlker
authors.initials: İ
authors.lastname: Akar
authors.affiliation: Department of Cardiovascular Surgery, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: İlker
authors.initials: İ
authors.lastname: İnce
authors.affiliation: Department of Cardiology, GATA Haydarpaşa Military Hospital, İstanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Yalçın
authors.affiliation: Department of Cardiology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Karayakalı
authors.affiliation: Department of Cardiology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Köksal
authors.initials: K
authors.lastname: Ceyhan
authors.affiliation: Department of Cardiology, Akdeniz University Faculty of Medicine, Antalya, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Koç
keywords: Atherosclerosis; C-reactive protein; interleukins; nitric oxide synthase; polymorphism