title: Bioaffinity Mass Spectrometry Screening.
abstract: Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS or ESI-FTMS) was used to screen 192 natural product extracts and a 659-member natural product-based fragment library for bindings to a potential malaria drug target, Plasmodium falciparum Rab11a (PfRab11a, PF13_0119). One natural product extract and 11 fragments showed binding activity. A new natural product, arborside E, was identified from the active extract of Psydrax montigena as a weak binder. Its binding activity and inhibitory activity against PfRab11a were confirmed by ESI-FTMS titration experiments and an orthogonal enzyme assay.
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia.
authors.forename: Ben
authors.initials: B
authors.lastname: Yang
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia.
authors.forename: Yun Jiang
authors.initials: YJ
authors.lastname: Feng
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia.
authors.forename: Hoan
authors.initials: H
authors.lastname: Vu
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia.
authors.forename: Brendan
authors.initials: B
authors.lastname: McCormick
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Rowley
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia.
authors.forename: Liliana
authors.initials: L
authors.lastname: Pedro
authors.affiliation: University of Washington, Seattle, WA, USA.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Crowther
authors.affiliation: University of Washington, Seattle, WA, USA.
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Van Voorhis
authors.affiliation: Queensland Herbarium, DSITI, Brisbane Botanic Gardens, Queensland, Australia.
authors.forename: Paul I
authors.initials: PI
authors.lastname: Forster
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia r.quinn@griffith.edu.au.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D010944:Plants / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D001688:Biological Products; D010936:Plant Extracts
keywords: FTMS; PfRab11a; malaria