title: Novel optical sensing film based on a functional nonwoven nanofibre mat for an easy, fast and highly selective and sensitive detection of tryptamine in beer.
abstract: In this paper, the combination of Solid Surface-Room Temperature Phosphorescence (SS-RTP) and nanotechnology has led to a new approach in the detection of biogenic amines in complex matrices. This novel approach allows, for the first time, the direct determination of the concentration of tryptamine in beers. The novelty of the proposed optical sensor resides in its simplicity, rapidity, absence of complex chromatographic separation, sample clean-up, preconcentration, and derivatization protocols. Therefore, this novel methodology simplifies and reduces considerably the time and cost of the analysis, resolving the two major problems of the determination of tryptamine in beer up to now: low sensitivity and matrix effects. The proposed sensor is based on a novel white, uncharged, and non-luminescent functional nonwoven nanofibre mat (Tiss®-Link) formed by hydrophilic nanofibres of 300 nm of diameter functionalized with a high concentration of active vinyl groups (330 µmol g(-1)). It is used to carry out a kinetically controlled covalent immobilisation of tryptamine via Michael type-reaction. The transduction of the sensor is phosphorescence; the covalently immobilized tryptamine is quantified by SS-RTP, obtaining a detection limit of 6 ng mL(-1) with short response times (15 min). The applicability of the sensor was demonstrated by analysing tryptamine in 10 different varieties of beers, obtaining recovery percentages close to 100%.
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ramon-Marquez
authors.affiliation: NanoMyP®, Nanomateriales y Polimeros S.L., Spin-Off company of the UGR, BIC building, Avd. Innovacion 1, E-18016 Granada, Spain. Electronic address: amedina@nanomyp.com.
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Medina-Castillo
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernandez-Gutierrez
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain. Electronic address: jffernan@ugr.es.
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Fernandez-Sanchez
mesh_terms: D001515:Beer / Q000032:analysis*; D005504:Food Analysis / Q000191:economics / Q000379:methods; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D014363:Tryptamines / Q000032:analysis*
chemical_list: D014363:Tryptamines; C030820:tryptamine
keywords: Beer; Nanofibre mat; Nanotechnology; Optical sensor; SS-RTP; Tryptamine