title: [Efficacy of RNA interference mediated by cationic liposomes].
abstract: To investigate the cytotoxicity of the homemade peptide cationic liposome CDO14 and its efficacy of RNA interference (RNAi). MTT method was used to determine the cytotoxicity of the liposome to a human lung cancer cell line Luc-A549 that can express luciferase stably. Luciferase siRNA (Luc-siRNA) was transfected into Luc-A549 cells by CDO14. Contents of luciferase in the transfected cells were detected by luminous instrument and contents of total protein in these cells were detected by BCA method. Nude mice were inoculated with Luc-A549 cells in axilla to establish xenograft tumor model. Complexes of Luc-siRNA and the cationic liposomes were injected into the modeling mice via tail vein. Contents of luciferase in the transfected mice were detected by the whole body imaging system. The cytotoxicity of the homemade cationic liposome was similar to that of commercial liposome DOTAP, and lower than that of Lipo2000. The siRNA transfection efficacy mediated by CDO14 was higher than that mediated by DOTAP. The homemade peptide cationic liposome CDO14 is expected to serve as delivery vector in gene therapy because of its low cytotoxicity and high transfection efficiency.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Han
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhen
authors.forename: Shubiao
authors.initials: S
authors.lastname: Zhang
authors.forename: Yinan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Enxia
authors.initials: E
authors.lastname: Wang
authors.forename: Zi
authors.initials: Z
authors.lastname: Liu
authors.forename: Yaoting
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D002412:Cations; D045744:Cell Line, Tumor; D005229:Fatty Acids, Monounsaturated; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes; D008156:Luciferases; D008175:Lung Neoplasms; D051379:Mice; D008819:Mice, Nude; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D034741:RNA, Small Interfering; D008156:Luciferases; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane