title: Phase Space Bottlenecks in Enzymatic Reactions.
abstract: The definition of a transition state on an individual reactive trajectory is made via a committor analysis. In the past, the bottleneck definition has often been applied in configuration space. This is an approximation, and in order to expand this definition, we are revisiting an enzyme in which we had identified a fast subpicosecond motion that makes the reaction possible. First we used a time-series analysis method to identify the exact time when this motion initiates donor-acceptor compression. Then we modified the standard committor analysis of transition path sampling to identify events in phase space and found that there is a dividing surface in phase space significantly earlier than the configurationally defined transition-state crossing.
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Antoniou
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Schwartz
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D008956:Models, Chemical
chemical_list: D004798:Enzymes