title: Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis typing of carbapenemases producing Acinetobacter baumannii isolated from burn patients.
abstract: BACKGROUND
  Acinetobacter baumannii is one of the important causes of nosocomial infection especially in burn patients. So, carbapenemase producing strains can make serious therapeutic problems. Molecular epidemiology studies play key role in decreasing the incidence of carbapenemase producing strains.
  
  
  METHODS
  In this study, 50 carbapenem resistant A. baumannii isolates from burn patients were collected. Antibiotic susceptibility testing and PCR assay was performed for detection of blaVIM, blaIMP, blaOXA-23,blaOXA-48,blaNDM-1, blaSPM-1, blaOxa-51 and blaKPC genes. Molecular typing for carbapenemase producing strains were performed by PFGE and MLVA.
  
  
  RESULTS
  According to the results of antibiotic susceptibility tests all of strains were XDR and 46 (92%) harbored carbapenemase genes. PFGE and MLVA results showed the presence of 11 and 5 dominant clonal types respectively.
  
  
  CONCLUSIONS
  The results of this study were showed the presence of certain clonal groups in two different wards of hospital indicating the spread of carbapenemase producing A. baumannii. On the other hand, the results showed the more discriminating power for PFGE.
authors.affiliation: Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Azimi
authors.affiliation: Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Malihe
authors.initials: M
authors.lastname: Talebi
authors.affiliation: Department of Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Khodaei
authors.affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Najafi
authors.affiliation: Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran. Electronic address: azizlari@gmail.com.
authors.forename: Abdolaziz Rastegar
authors.initials: AR
authors.lastname: Lari
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002056:Burns / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests; D018598:Minisatellite Repeats / Q000235:genetics*; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: A. baumannii; MLVA; PFGE