title: Curcumin-Loading-Dependent Stability of PEGMEMA-Based Micelles Affects Endocytosis and Exocytosis in Colon Carcinoma Cells.
abstract: Polymeric micelles were formed from poly(poly(ethylene glycol) methyl ether methacrylate)-block-poly(styrene) (P(PEGMEMA)-b-PS) block copolymer of two different chain lengths. The micelles formed were approximately 16 and 46 nm in diameter and used to encapsulate curcumin. Upon loading of the curcumin into the micelles, their size increased to approximately 34 and 80 nm in diameter, respectively, with a loading efficiency of 58%. The unloaded micelles were not cytotoxic to human colon carcinoma cells, whereas only the smaller loaded micelles were cytotoxic after 72 h of exposure. The micelles were rapidly internalized by the cells within minutes of exposure, with the loaded micelles internalized to a greater extent owing to their enhanced stability compared to that of the unloaded micelles. The larger micelles were more rapidly internalized and exocytosed than the smaller micelles, demonstrating the effect of micelle size and drug loading on drug delivery and cytotoxicity.
authors.affiliation: Graduate School of Biomedical Engineering and ‡Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Sydney, NSW 2052, Australia.
authors.forename: Teddy
authors.initials: T
authors.lastname: Chang
authors.affiliation: Graduate School of Biomedical Engineering and ‡Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Sydney, NSW 2052, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Trench
authors.affiliation: Graduate School of Biomedical Engineering and ‡Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Sydney, NSW 2052, Australia.
authors.forename: Joshua
authors.initials: J
authors.lastname: Putnam
authors.affiliation: Graduate School of Biomedical Engineering and ‡Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Sydney, NSW 2052, Australia.
authors.forename: Martina H
authors.initials: MH
authors.lastname: Stenzel
authors.affiliation: Graduate School of Biomedical Engineering and ‡Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Sydney, NSW 2052, Australia.
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lord
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D008823:Micelles; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008689:Methacrylates; D008823:Micelles; D011108:Polymers; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D003474:Curcumin
keywords: cancer; cell uptake; curcumin; drug delivery; endocytosis; exocytosis; flow cytometry; live cell imaging; nanoparticles; polymeric micelles