title: Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling.
abstract: BACKGROUND
  Women with triple negative breast cancers (TNBCs) have a poor prognosis due to lack of suitable targeted therapies. Changes in the protein glycosylation are increasingly being recognized as an important modification associated with cancer etiology.
  
  
  METHODS
  In an attempt to identify TNBC biomarkers with greater diagnostic and prognostic capabilities, hydrazide- based chemistry method combined with LC-MS/MS were used to purify and identify N-linked glycopeptides or glycoproteins of tissues from TNBC patients.
  
  
  RESULTS
  A total of 550 unique N-linked glycoproteins were identified, among these proteins, 72 unique N-linked glycoproteins were significantly regulated in tumor tissues, of which 56 proteins were upregulated and 16 proteins were downregulated. To assess the validity of the results, three selected proteins including Vascular endothelial growth factor receptor 1, Insulin receptor, Tissue factor pathway inhibitor were selected for western blot analysis, and these proteins were found as potential biomarkers of TNBC. The top three pathways of differentially expressed glycoproteins participated in were caveolar-mediated endocytosis signaling, agrin interactions at neuromuscular junction and LXR/RXR activation.
  
  
  CONCLUSIONS
  This work provides potential glycoprotein markers to function as a novel tissue-based biomarker for TNBC.
authors.affiliation: Breast Center of Jiangsu Province, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Jindao
authors.initials: J
authors.lastname: Wu
authors.forename: Huaxing
authors.initials: H
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
authors.forename: Xiaoan
authors.initials: X
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zha
authors.forename: Mengdi
authors.initials: M
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Shui
authors.initials: S
authors.lastname: Wang
authors.forename: Tiansong
authors.initials: T
authors.lastname: Xia
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling; D008074:Lipoproteins / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008875:Middle Aged; D040901:Proteomics; D011972:Receptor, Insulin / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D006020:Glycopeptides; D006023:Glycoproteins; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; D011972:Receptor, Insulin; D040281:Vascular Endothelial Growth Factor Receptor-1