title: Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing.
abstract: An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3'-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis.
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. yumeishen@sjtu.edu.cn.
authors.forename: Lianjiang
authors.initials: L
authors.lastname: Tan
authors.forename: Yazhi
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Gong
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Shen
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000143:Acids / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D058105:Polymerization; D017422:Sequence Analysis, DNA
chemical_list: D000143:Acids; D005456:Fluorescent Dyes; D009711:Nucleotides