title: Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis.
abstract: MicroRNAs (miRNAs) are a group of short, noncoding, regulatory RNA molecules the dysregulation of which contributes to the pathogenesis of myocarditis. Argonaute proteins are essential components of miRNA-induced silencing complex and play important roles during miRNA biogenesis and function. However, the expression pattern of four AGO family members has not yet been detected in the coxsackievirus B3 (CVB3)-induced myocarditis tissue samples. In this study, we detected the expression of four AGOs in the CVB3-infected mouse heart tissues and found that AGO1 and AGO3 up-regulated significantly at 4 and 8h after CVB3 infection. Further in vitro research indicated that up-regulated AGO1 and AGO3 are related to the down-regulated TNFAIP3, which is a negative regulator of NF-κB pathway. Subsequently, we confirmed that TNFAIP3 is a direct target of miR-19a/b, and during CVB3 infection, the expression of miR-19a/b and miR-125a/b is not significantly changed. TNFAIP3 level is mainly reduced by up-regulated AGO1 and AGO3. This research sheds light on the relationship between overexpressed AGO proteins and CVB3-induced myocarditis, and this provides potential therapeutic target for viral myocarditis.
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Shougang
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Pingliang First People's Hospital, Pingliang, 744000, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Qiongying
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Nephrology, the 306th Hospital of the Chinese PLA, Beijing 100101, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Bai
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chang
authors.affiliation: Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Cardiac Surgery, Lanzhou University Second Hospital, Lanzhou, 730030, China. Electronic address: bingrengao@yeah.net.
authors.forename: Bingren
authors.initials: B
authors.lastname: Gao
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism*; D015153:Blotting, Western; D003384:Coxsackievirus Infections / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D029822:Enterovirus B, Human; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D009205:Myocarditis / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D059467:Transcriptome
chemical_list: D060565:Argonaute Proteins; D016328:NF-kappa B
keywords: Argonaute; Coxsackievirus; MicroRNA; Myocarditis; TNFAIP3