title: A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium.
abstract: A novel heterotrophic bacterium capable of heterotrophic nitrification and aerobic denitrification was isolated from ammonium contaminated landfill leachate and physiochemical and phylogenetically identified as Zobellella taiwanensis DN-7. DN-7 converted nitrate, nitrate, and ammonium to N2 as the primary end product. Single factor experiments suggested that the optimal conditions for ammonium removal were trisodium citrate as carbon source, C/N ratio 8, pH 8.0-10.0, salinity less than 3 %, temperature 30 °C, and rotation speed more than 150 rpm. Specifically, DN-7 could remove 1000.0 and 2000.0 mg/L NH4 (+)-N completely within 96 and 216 h, with maximum removal rates of 19.6 and 17.3 mg L(-1) h(-1), respectively. These results demonstrated that DN-7 is a promising candidate for application of high-strength ammonium wastewater treatments.
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing, 400714, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing, 400714, China.
authors.forename: Yangqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing, 400714, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, 48109, USA.
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Xi
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing, 400714, China. songliyan@cigit.ac.cn.
authors.forename: Liyan
authors.initials: L
authors.lastname: Song
mesh_terms: D000332:Aerobiosis; D044044:Aeromonadaceae / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D064751:Ammonium Compounds / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D002244:Carbon / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D058440:Denitrification; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen; D010802:Phylogeny; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D064751:Ammonium Compounds; D002951:Citrates; D009566:Nitrates; D009573:Nitrites; C514290:trisodium citrate; D002244:Carbon; D009584:Nitrogen
keywords: Aerobic denitrification; Heterotrophic nitrification; High-strength ammonium removal; Zobellella taiwanensis DN-7