title: Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions.
abstract: Vitrification could provide a promising tool for the cryopreservation of fish embryos. To achieve successful cryopreservation, several parameters should be taken into account in the design of a vitrification protocol. In the present study, some relevant factors were investigated (choice of a proper vitrificant solutions and temperature for thawing) using neurulation-stage embryos. Six DMSO-based vitrificant solutions (V1-V6) were tested using a 6-step incorporation protocol. DMSO-based vitrificant solutions contained DMSO + permeable cryoprotectants + nonpermeable cryoprotectants. Embryos were immersed in vitrificant solutions for 7 minutes and directly plunged into liquid nitrogen. After vitrification (-196 °C for 10 minutes), the thawing was performed in a water bath at 0 or 20 °C and then embryos incubated until hatched. Our results demonstrated that some embryos vitrified in 5 of 6 vitrification solutions survived and hatched out, but none survived after vitrification in V2. The highest survival rate (45.45%) was observed in samples frozen with the best vitrificant solution (V6) and thawing combination (20 °C). These results establish that cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions is possible.
authors.affiliation: Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: skeivanloo@gau.ac.ir.
authors.forename: Saeide
authors.initials: S
authors.lastname: Keivanloo
authors.affiliation: Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sudagar
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000187:drug effects; D005399:Fishes / Q000196:embryology*; D005615:Freezing; D058989:Vitrification / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide
keywords: Acipenser persicus; Cryopreservation; DMSO; Embryo; Persian sturgeon