title: Protective effects of carnosine alone and together with alpha-tocopherol on lipopolysaccharide (LPS) plus ethanol-induced liver injury.
abstract: The aim of this study was to investigate the effect of carnosine (CAR) alone and together with vitamin E (Vit E) on alcoholic steatohepatitis (ASH) in rats. ASH was induced by ethanol (3 times; 5 g/kg; 12 h intervals, via gavage), followed by a single dose of lipopolysaccharide (LPS; 10 mg/kg; i.p.). CAR (250 mg/kg; i.p.) and Vit E (200 mg D-α-tocopherol/kg; via gavage) were administered 30 min before and 90 min after the LPS injection. CAR treatment lowered high serum transaminase activities together with hepatic histopathologic improvements in rats with ASH. Reactive oxygen species formation, malondialdehyde levels, myeloperoxidase activities and transforming growth factor β1 (TGF-β1) and collagen 1α1 (COL1A1) expressions were observed to decrease. These improvements were more remarkable in CAR plus Vit E-treated rats. Our results indicate that CAR may be effective in suppressing proinflammatory, prooxidant, and profibrotic factors in the liver of rats with ASH.
authors.affiliation: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, Istanbul, Turkey.
authors.forename: Esra Betül
authors.initials: EB
authors.lastname: Kalaz
authors.affiliation: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, Istanbul, Turkey.
authors.forename: A Fatih
authors.initials: AF
authors.lastname: Aydın
authors.affiliation: Department of Pathology, Yeditepe University Medical Faculty, Kayışdağı, Istanbul, Turkey.
authors.forename: Işın
authors.initials: I
authors.lastname: Doğan-Ekici
authors.affiliation: Department of Biochemistry, Yeditepe University Medical Faculty, Kayışdağı, Istanbul, Turkey.
authors.forename: Jale
authors.initials: J
authors.lastname: Çoban
authors.affiliation: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, Istanbul, Turkey. Electronic address: sdabbasoglu@yahoo.com.
authors.forename: Semra
authors.initials: S
authors.lastname: Doğru-Abbasoğlu
authors.affiliation: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, Istanbul, Turkey.
authors.forename: Müjdat
authors.initials: M
authors.lastname: Uysal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002336:Carnosine / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D000431:Ethanol; D008315:Malondialdehyde; D002336:Carnosine; D024502:alpha-Tocopherol
keywords: Carnosine; Ethanol; Lipopolysaccharide; Liver; Oxidative stress; Vitamin E