title: Analysis of circulating CD14+/CD16+ monocyte-derived macrophages (MDMs) in the peripheral blood of patients with oral squamous cell carcinoma.
abstract: OBJECTIVE
  Monocytes/macrophages are regarded as the first line of defense in tumors. Therefore, analyzing monocyte subtypes in oral squamous cell carcinoma (OSCC) may be of value in disease monitoring and to explore immunotherapeutic strategies for cancer patients.
  
  
  METHODS
  Circulating peripheral blood CD14+/CD16+ monocyte-derived macrophages (MDMs) were evaluated in OSCC patients with oral squamous cell carcinoma (n = 44) compared with controls (n = 85). Moreover, epitope detection in monocytes (EDIM) technology was used to detect biomarkers Apo10 and transketolase-like-1 in CD14+/CD16+ MDMs.
  
  
  RESULTS
  Compared with controls, no significant (P = .3646) difference (control group 9.8%, OSCC group 8.8%) in CD14+/CD16+ MDM were noted in OSCC. However, EDIM-Apo10 and EDIM-TKTL1 scores detected in the CD14+/CD16+ MDMs were increased in OSCC compared with controls (P < .0001).
  
  
  CONCLUSIONS
  Analyzing CD14+/CD16+ MDMs represents a stable cell population for detecting biomarkers in cancer disease monitoring.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital Tuebingen, 72076 Tuebingen, Germany. Electronic address: dr.dr.martingrimm@googlemail.com.
authors.forename: Martin
authors.initials: M
authors.lastname: Grimm
authors.affiliation: Zyagnum AG, 64319 Pfungstadt, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Feyen
authors.affiliation: Zyagnum AG, 64319 Pfungstadt, Germany.
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Coy
authors.affiliation: Biovis' Diagnostik MVZ, 65555 Limburg an der Lahn, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Hofmann
authors.affiliation: Cancer Research Center, Sanford-Burnham Medical Research Institute, La Jolla, CA, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Teriete
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital Tuebingen, 72076 Tuebingen, Germany.
authors.forename: Siegmar
authors.initials: S
authors.lastname: Reinert
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000097:blood / Q000276:immunology; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood* / Q000276:immunology; D008207:Lymphatic Metastasis; D008264:Macrophages / Q000145:classification* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000145:classification* / Q000276:immunology; D009062:Mouth Neoplasms / Q000097:blood* / Q000276:immunology / Q000473:pathology; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D017452:Receptors, IgG / Q000097:blood* / Q000276:immunology
chemical_list: D014408:Biomarkers, Tumor; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG