title: Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection.
abstract: This study was conducted to examine the effects of eggshell membrane hydrolysates (ESMH) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. Whole ESMH (before fractionation), and fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) of the hydrolysates were assessed in this experiment. As lipopolysaccharide (LPS) and IFN-γ caused the inflammation on Raw264.7 cell, whole ESMH and fraction I showed to be effective in inhibiting the induction of cell inflammation depending on the concentration, and also showed outstanding effect to suppress the skin inflammation. Fraction I inhibited collagenase and elastase activities to a greater extent than the other fractions, while all fractions had antibiotic effects at concentrations of 10 mg/disc and 20 mg/disc. In addition, it showed the moisture protection effects of skin on the holding amount and losing amount of moisture in upper-inner arm of the human body with a relatively low loss rate in skin, which confirmed that the hydrolyzed fractions of ESM helps to form the superior protective layer of moisture. It was concluded that ESMH fractions with different molecular weights, especially the 10 kDa fraction, have anti-lipopolysaccharide, anti-IFN-γ-induced inflammation, anti- collagenase and elastase activities, and thus can be used as a cosmetic agent to protect skin.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Jinhee
authors.initials: J
authors.lastname: Yoo
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Kimoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Youngji
authors.initials: Y
authors.lastname: Yoo
authors.affiliation: Sungkyun Biotech Co., LTD R&D Center, Ansan 425-839, Korea.
authors.forename: Jongkeun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Sungkyun Biotech Co., LTD R&D Center, Ansan 425-839, Korea.
authors.forename: Heejin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Environmental Horticulture, Dankook University, Cheonan 330-714, Korea.
authors.forename: Youngjae
authors.initials: Y
authors.lastname: Shin
keywords: anti-aging; anti-inflammation; antibiotic; eggshell membrane; moisture protection