title: [Flow Cytometric Analysis of Genome Size in Atractylodes lancea].
abstract: OBJECTIVE
  To develop a rapid analytical method for the determination of genome size of Atractylodes lancea by flow cytometry (FCM), and to estimate genome size of five typical plant types of cultivated Atractylodes lancea.
  
  
  METHODS
  The fresh young leaves of Atractylodes lancea were used for the preparation of nuclear suspension with two-step protocol. After staining with propidium iodide, the mixture was analyzed by flow cytometry. Zea mays 'CE-777' or Vicafaba 'Inorce' was used for DNA reference standard.
  
  
  RESULTS
  The flow cytometric method was developed after screening internal standard, optimizing sample preparation and FCM setups. The narrow leaf type of Atractylodes lancea got the biggest genome size, whereas the smallest one was fasciated stem type. It was suggested that parted leaf might be caused by genome size decrease.
  
  
  CONCLUSIONS
  The methodology presented in this study is suitable for measuring the genome size of Atractylodes lancea. This study also provides useful information on population variation, evaluation of germplasm resources, and breeding of Atractylodes lancea.
authors.forename: Jin-jing
authors.initials: JJ
authors.lastname: Ni
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Yu
mesh_terms: D031174:Atractylodes / Q000235:genetics*; D018744:DNA, Plant; D005434:Flow Cytometry; D059646:Genome Size; D018515:Plant Leaves; D013194:Staining and Labeling
chemical_list: D018744:DNA, Plant