title: The DJ-1 superfamily members YhbO and YajL from Escherichia coli repair proteins from glycation by methylglyoxal and glyoxal.
abstract: YhbO and YajL belong to the PfpI/Hsp31/DJ-1 superfamily. Both proteins are involved in protection against environmental stresses. Here, we show that, like DJ-1 and Hsp31, they repair glyoxal- and methylglyoxal-glycated proteins. YhbO and YajL repair glycated serum albumin, collagen, glyceraldehyde-3-phosphate dehydrogenase, and fructose biphosphate aldolase. Bacterial extracts from deglycase mutants display increased glycation levels, whereas deglycase overexpression decreases protein glycation. Moreover, yhbO and yajL mutants display decreased viability in methylglyoxal- or glucose-containing media. Finally, the apparent glyoxalase activities of YhbO and YajL reflect their deglycase activities.
authors.affiliation: Stress Molecules, Institut Jacques Monod, Université Paris 7, CNRS UMR 7592, 15 rue Hélène Brion, 75013 Paris, France; Lebanese American University, School of Pharmacy, Byblos, Lebanon.
authors.forename: Jad
authors.initials: J
authors.lastname: Abdallah
authors.affiliation: Stress Molecules, Institut Jacques Monod, Université Paris 7, CNRS UMR 7592, 15 rue Hélène Brion, 75013 Paris, France.
authors.forename: Mouadh
authors.initials: M
authors.lastname: Mihoub
authors.affiliation: Stress Molecules, Institut Jacques Monod, Université Paris 7, CNRS UMR 7592, 15 rue Hélène Brion, 75013 Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gautier
authors.affiliation: Stress Molecules, Institut Jacques Monod, Université Paris 7, CNRS UMR 7592, 15 rue Hélène Brion, 75013 Paris, France. Electronic address: richarme@paris7.jussieu.fr.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Richarme
mesh_terms: D019610:Cytoprotection / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006037:Glyoxal / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D017127:Glycation End Products, Advanced; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012269:Ribosomal Proteins; C554198:YajL protein, E coli; C513540:YhbO protein, E coli; C469996:hchA protein, E coli; D006037:Glyoxal; D011765:Pyruvaldehyde
keywords: Carbonyl stress; Electrophile stress; Glycation; Maillard reaction; Protein repair