title: Association of O-Antigen Serotype with the Magnitude of Initial Systemic Cytokine Responses and Persistence in the Urinary Tract.
abstract: Urinary tract infection (UTI) is one of the most common ailments requiring both short-term and prophylactic antibiotic therapies. Progression of infection from the bladder to the kidney is associated with more severe clinical symptoms (e.g., fever and vomiting) as well as with dangerous disease sequelae (e.g., renal scaring and sepsis). Host-pathogen interactions that promote bacterial ascent to the kidney are not completely understood. Prior studies indicate that the magnitude of proinflammatory cytokine elicitation in vitro by clinical isolates of uropathogenic Escherichia coli (UPEC) inversely correlates with the severity of clinical disease. Therefore, we hypothesize that the magnitude of initial proinflammatory responses during infection defines the course and severity of disease. Clinical UPEC isolates obtained from patients with a nonfebrile UTI elicited high systemic proinflammatory responses early during experimental UTI in a murine model and were attenuated in bladder and kidney persistence. Conversely, UPEC isolates obtained from patients with febrile UTI elicited low systemic proinflammatory responses early during experimental UTI and exhibited prolonged persistence in the bladder and kidney. Soluble factors in the supernatant from saturated cultures as well as the lipopolysaccharide (LPS) serotype correlated with the magnitude of proinflammatory responses in vitro. Our data suggest that the structure of the O-antigen sugar moiety of the LPS may determine the strength of cytokine induction by epithelial cells. Moreover, the course and severity of disease appear to be the consequence of the magnitude of initial cytokines produced by the bladder epithelium during infection.
  
  
  OBJECTIVE
  The specific host-pathogen interactions that determine the extent and course of disease are not completely understood. Our studies demonstrate that modest changes in the magnitude of cytokine production observed using in vitro models of infection translate into significant ramifications for bacterial persistence and disease severity. While many studies have demonstrated that modifications of the LPS lipid A moiety modulate the extent of Toll-like receptor 4 (TLR4) activation, our studies implicate the O-antigen sugar moiety as another potential rheostat for the modulation of proinflammatory cytokine production.
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: Horvath
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Ashay S
authors.initials: AS
authors.lastname: Patel
authors.affiliation: Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Mohamed
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Douglas W
authors.initials: DW
authors.lastname: Storm
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Chandra
authors.initials: C
authors.lastname: Singh
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Birong
authors.initials: B
authors.lastname: Li
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Nationwide Children's Hospital, Columbus, Ohio, USA The Ohio State University School of Medicine, Columbus, Ohio, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Koff
authors.affiliation: Nationwide Children's Hospital, Columbus, Ohio, USA The Ohio State University School of Medicine, Columbus, Ohio, USA.
authors.forename: Venkata R
authors.initials: VR
authors.lastname: Jayanthi
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA The Ohio State University School of Medicine, Columbus, Ohio, USA.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Mason
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA The Ohio State University School of Medicine, Columbus, Ohio, USA Sheryl.justice@nationwidechildrens.org.
authors.forename: Sheryl S
authors.initials: SS
authors.lastname: Justice
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D006801:Humans; D051379:Mice; D019081:O Antigens / Q000145:classification / Q000276:immunology*; D065288:Serogroup; D014551:Urinary Tract / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D014552:Urinary Tract Infections / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D056689:Uropathogenic Escherichia coli / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D016207:Cytokines; D019081:O Antigens