title: New strategies for the use of Linum usitatissimum cell factories for the production of bioactive compounds.
abstract: In this work, suspension-cultured cells of Linum usitatissimum L. were used to evaluate the effect of two types of cyclodextrins, β-glucan and (Z)-3-hexenol separately or in combination on phytosterol and tocopherol production. Suspension-cultured cells of L. usitatissimum were able to produce high levels of phytosterols in the presence of 50 mM methylated-β-cyclodextrins (1325.96 ± 107.06 μg g dry weight(-1)) separately or in combination with β-glucan (1278.57 ± 190.10 μg g dry weight(-1)) or (Z)-3-hexenol (1507.88 ± 173.02 μg g dry weight(-1)), being cyclodextrins able to increase both the secretion and accumulation of phytosterols in the spent medium, whereas β-glucan and (Z)-3-hexenol themselves only increased its intracellular accumulation. Moreover, the phytosterol values found in the presence of hydroxypropylated-β-cyclodextrins were lower than those found in the presence of methylated-β-cyclodextrins in all cases studied. However, the results showed that the presence of methylated-β-cyclodextrins did not increase the tocopherols production and only an increase in tocopherol levels was observed when cells were elicited with 50 mM hydroxypropylated-β-cyclodextrins in combination with β-glucan (174 μg g dry weight(-1)) or (Z)-3-hexenol (257 μg g dry weight(-1)). Since the levels of tocopherol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. To sum up, flax cell cultures elicited with cyclodextrins alone or in combination with β-glucan or (Z)-3-hexenol were able produce phytosterols and tocopherols, and therefore, these elicited suspension-cultured cells of L. usitatissimum can provide an alternative system, which is at the same time more sustainable, economical and ecological for their production.
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100, Murcia, Spain. Electronic address: lorena.almagro@um.es.
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100, Murcia, Spain.
authors.forename: Pascual
authors.initials: P
authors.lastname: García-Pérez
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100, Murcia, Spain.
authors.forename: Sarai
authors.initials: S
authors.lastname: Belchí-Navarro
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100, Murcia, Spain.
authors.forename: Pedro Joaquín
authors.initials: PJ
authors.lastname: Sánchez-Pujante
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100, Murcia, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Pedreño
mesh_terms: D019149:Bioreactors / Q000592:standards; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003505:Cyclodextrins / Q000494:pharmacology; D004357:Drug Synergism; D019597:Flax / Q000187:drug effects / Q000378:metabolism*; D000441:Hexanols / Q000494:pharmacology; D010840:Phytosterols / Q000096:biosynthesis*; D024505:Tocopherols / Q000378:metabolism*; D047071:beta-Glucans / Q000494:pharmacology
chemical_list: D003505:Cyclodextrins; D000441:Hexanols; D010840:Phytosterols; D047071:beta-Glucans; D024505:Tocopherols
keywords: (Z)-3-hexenol; Cyclodextrins; Flax; Phytosterols; Suspension-cultured cell; Tocopherols; β-glucan