title: Polymorphisms in the cytochrome P-450 (CYP) 1A1 and 17 genes are not associated with acne vulgaris in the Polish population.
abstract: BACKGROUND
  The pathogenesis of acne is complex, multifactorial and not well understood. The genetic background of this dermatosis is well documented.
  
  
  OBJECTIVE
  To assess the frequency of -34 T > C single nucleotide polymorphism in the promoter of the CYP17 gene as well as m1 (+6,235 T > C) and m2 (+4,889 A > G) mutation in the coding region CYP1A1 gene acne patients from the Northern Polish population.
  
  
  METHODS
  The study included 115 acne patients and 94 healthy controls (aged over 20) without acne in anamnesis. The CYP1A1 polymorphism was analyzed by polymerase chain reaction (PCR). The restriction fragment length polymorphism (RFLP) was used to analyze m1 mutation and allele-specific PCR in the case of m2 mutation. The CYP17 polymorphism was analyzed by RFLP. The results were evaluated by the Pearson's χ(2) test.
  
  
  RESULTS
  There were no statistically significant associations between allele and genotype frequencies between the acne and the control group.
  
  
  CONCLUSIONS
  We did not confirm the role of the CYP1A1 and CYP17 gene as predictor factors for acne development in the Polish population.
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Prof. Roman Nowicki MD, PhD.
authors.forename: Michał
authors.initials: M
authors.lastname: Sobjanek
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Prof. Roman Nowicki MD, PhD.
authors.forename: Monika
authors.initials: M
authors.lastname: Zabłotna
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Prof. Roman Nowicki MD, PhD.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dobosz-Kawałko
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Prof. Roman Nowicki MD, PhD.
authors.forename: Igor
authors.initials: I
authors.lastname: Michajłowski
authors.affiliation: Department of Nursing, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Andrzej Chamienia MD, PhD.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Mędrzycka-Dąbrowska
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Prof. Roman Nowicki MD, PhD.
authors.forename: Roman
authors.initials: R
authors.lastname: Nowicki
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland. Head of the Department: Prof. Roman Nowicki MD, PhD.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sokołowska-Wojdyło
keywords: CYP17; CYP1A1; acne; cytochrome P-450; gene polymorphism; genetic factors