title: Chloroplast Activity and 3'phosphadenosine 5'phosphate Signaling Regulate Programmed Cell Death in Arabidopsis.
abstract: Programmed cell death (PCD) is a crucial process both for plant development and responses to biotic and abiotic stress. There is accumulating evidence that chloroplasts may play a central role during plant PCD as for mitochondria in animal cells, but it is still unclear whether they participate in PCD onset, execution, or both. To tackle this question, we have analyzed the contribution of chloroplast function to the cell death phenotype of the myoinositol phosphate synthase1 (mips1) mutant that forms spontaneous lesions in a light-dependent manner. We show that photosynthetically active chloroplasts are required for PCD to occur in mips1, but this process is independent of the redox state of the chloroplast. Systematic genetic analyses with retrograde signaling mutants reveal that 3'-phosphoadenosine 5'-phosphate, a chloroplast retrograde signal that modulates nuclear gene expression in response to stress, can inhibit cell death and compromises plant innate immunity via inhibition of the RNA-processing 5'-3' exoribonucleases. Our results provide evidence for the role of chloroplast-derived signal and RNA metabolism in the control of cell death and biotic stress response.
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Quentin
authors.identifier: http://orcid.org/0000-0002-2288-7744
authors.initials: Q
authors.lastname: Bruggeman
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Christelle
authors.initials: C
authors.lastname: Mazubert
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Florence
authors.initials: F
authors.lastname: Prunier
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Raphaël
authors.identifier: http://orcid.org/0000-0002-1238-844X
authors.initials: R
authors.lastname: Lugan
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Kai Xun
authors.initials: KX
authors.lastname: Chan
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Su Yin
authors.identifier: http://orcid.org/0000-0002-7556-1211
authors.initials: SY
authors.lastname: Phua
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Barry James
authors.initials: BJ
authors.lastname: Pogson
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Anja
authors.initials: A
authors.lastname: Krieger-Liszkay
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Marianne
authors.initials: M
authors.lastname: Delarue
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Moussa
authors.initials: M
authors.lastname: Benhamed
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
authors.forename: Catherine
authors.initials: C
authors.lastname: Bergounioux
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.) cecile.raynaud@u-psud.fr.
authors.forename: Cécile
authors.initials: C
authors.lastname: Raynaud
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance / Q000235:genetics; D009154:Mutation; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics; D044224:Pseudomonas syringae / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
chemical_list: D002734:Chlorophyll; D000244:Adenosine Diphosphate; C010840:adenosine 3'-phosphate-5'-phosphate; D007296:Myo-Inositol-1-Phosphate Synthase