title: Evaluation of allergenic potential for rice seed protein components utilizing a rice proteome database and an allergen database in combination with IgE-binding of recombinant proteins.
abstract: Among 131 rice endosperm proteins previously identified by MS-based proteomics, most of the proteins showed low or almost no sequence similarity to known allergens in databases, whereas nine proteins did it significantly. The sequence of two proteins showed high overall identity with Hsp70-like hazel tree pollen allergen (Cor a 10) and barley α-amylase (Hor v 16), respectively, whereas the others showed low identity (28-58%) with lemon germin-like protein (Cit l 1), corn zein (Zea m 50 K), wheat chitinase-like xylanase inhibitor (Tri a XI), and kinase-like pollen allergen of Russian thistle (Sal k 1). Immuno-dot blot analysis showed that recombinant proteins for these rice seed homologs were positive in the IgE-binding, but not necessarily similarity dependent, from some allergic patients. These results suggest that utilization of proteome and sequence databases in combination with IgE-binding analysis was effective to screen and evaluate allergenic potential of rice seed protein components.
authors.affiliation: a Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya , Japan.|b Department of Food and Nutritional Environment , College of Human Life Environment, Kinjo Gakuin University , Nagoya , Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Hirano
authors.affiliation: a Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya , Japan.|c Faculty of Agriculture, Department of Applied Biological Chemistry , Shizuoka University , Shizuoka , Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Hino
authors.affiliation: a Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Kenzi
authors.initials: K
authors.lastname: Oshima
authors.affiliation: a Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
authors.affiliation: d Department of Pediatrics , Fujita Health University, The Second Teaching Hospital , Nagoya , Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Urisu
authors.affiliation: e Transgenic Crop Research and Development Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
authors.affiliation: a Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000485:Allergens / Q000276:immunology*; D030562:Databases, Protein; D007073:Immunoglobulin E / Q000276:immunology*; D012275:Oryza / Q000196:embryology / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D020543:Proteome; D011994:Recombinant Proteins / Q000276:immunology; D012639:Seeds / Q000276:immunology*
chemical_list: D000485:Allergens; D010940:Plant Proteins; D020543:Proteome; D011994:Recombinant Proteins; D007073:Immunoglobulin E
keywords: allergen database; food allergy; rice proteome; rice seed