title: α-Tocopherol succinate improves encapsulation and anticancer activity of doxorubicin loaded in solid lipid nanoparticles.
abstract: This work aimed to develop solid lipid nanoparticles (SLN) co-loaded with doxorubicin and α-tocopheryl succinate (TS), a succinic acid ester of α-tocopherol that exhibits anticancer actions, evaluating the influence of TS on drug encapsulation efficiency. The SLN were characterized for size, zeta potential, entrapment efficiency (EE), and drug release. Studies of in vitro anticancer activity were also conducted. The EE was significantly improved from 30 ± 1% to 96 ± 2% for SLN without and with TS at 0.4%, respectively. In contrast, a reduction in particle size from 298 ± 1 to 79 ± 1 nm was observed for SLN without and with TS respectively. The doxorubicin release data show that SLN provide a controlled drug release. The in vitro studies showed higher cytotoxicity for doxorubicin-TS-loaded SLN than for free doxorubicin in breast cancer cells. These findings suggest that TS-doxorubicin-loaded SLN is a promising alternative for the treatment of cancer.
authors.affiliation: Department of Phamaceutics, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Brazil.
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Oliveira
authors.affiliation: Department of Phamaceutics, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Brazil.
authors.forename: Samuel V
authors.initials: SV
authors.lastname: Mussi
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Brazil.
authors.forename: Dawidson A
authors.initials: DA
authors.lastname: Gomes
authors.affiliation: Department of Chemistry, Institute of Exact Sciences, Federal University of Minas Gerais (UFMG), Brazil.
authors.forename: Maria Irene
authors.initials: MI
authors.lastname: Yoshida
authors.affiliation: Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Av Antônio Carlos, 6627, Campus Pampulha, 31270-901 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Frederic
authors.initials: F
authors.lastname: Frezard
authors.affiliation: Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Av Antônio Carlos, 6627, Campus Pampulha, 31270-901 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Virgínia M
authors.initials: VM
authors.lastname: Carregal
authors.affiliation: Department of Phamaceutics, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Brazil. Electronic address: lucas@farmacia.ufmg.br.
authors.forename: Lucas A M
authors.initials: LAM
authors.lastname: Ferreira
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D004339:Drug Compounding; D065546:Drug Liberation; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D061986:MCF-7 Cells; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D024502:alpha-Tocopherol / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D008055:Lipids; D004317:Doxorubicin; D024502:alpha-Tocopherol
keywords: Cancer; Cytotoxicity; Doxorubicin; Solid lipid nanoparticle; α-Tocopherol succinate