title: Tempol, a Membrane-Permeable Radical Scavenger, Exhibits Anti-Inflammatory and Cardioprotective Effects in the Cerulein-Induced Pancreatitis Rat Model.
abstract: To date, it remains unclear whether mild form of acute pancreatitis (AP) may cause myocardial damage which may be asymptomatic for a long time. Pathogenesis of AP-related cardiac injury may be attributed in part to ROS/RNS overproduction. The aim of the present study was to evaluate the oxidative stress changes in both the pancreas and the heart and to estimate the protective effects of 1-oxyl-2,2,6,6-tetramethyl-4-hydroxypiperidine (tempol) at the early phase of AP. Cerulein-induced AP led to the development of acute edematous pancreatitis with a significant decrease in the level of sulfhydryl (-SH) groups (oxidation marker) both in heart and in pancreatic tissues as well as a substantial increase in plasma creatine kinase isoenzyme (CK-MB) activity (marker of the heart muscle lesion) which confirmed the role of oxidative stress in the pathogenesis of cardiac damage. The tempol treatment significantly reduced the intensity of inflammation and oxidative damage and decreased the morphological evidence of pancreas injury at early AP stages. Moreover, it markedly attenuated AP-induced cardiac damage revealed by normalization of the -SH group levels and CK-MB activity. On the basis of these studies, it is possible to conclude that tempol has a profound protective effect against cardiac and pancreatic damage induced by AP.
authors.affiliation: Chair and Department of Pathophysiology, Medical University of Lublin, 20-090 Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marciniak
authors.affiliation: Chair and Department of Clinical Pathomorphology, Medical University of Lublin, 20-090 Lublin, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Walczyna
authors.affiliation: Department of Pharmacology, Medical University of Lublin, 20-093 Lublin, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Rajtar
authors.affiliation: Department of Pharmacology, Medical University of Lublin, 20-093 Lublin, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Marciniak
authors.affiliation: Chair and Department of Vascular Surgery and Angiology, Medical University of Lublin, 20-081 Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojtak
authors.affiliation: Department of Pharmacology, University of Life Sciences in Lublin, 20-033 Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Lasiecka
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002108:Ceruletide; D052279:Creatine Kinase, MB Form / Q000097:blood; D003497:Cyclic N-Oxides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D010195:Pancreatitis / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy* / Q000201:enzymology; D017208:Rats, Wistar; D013113:Spin Labels; D014867:Water / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002316:Cardiotonic Agents; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D013113:Spin Labels; D014867:Water; D002108:Ceruletide; D052279:Creatine Kinase, MB Form; D000681:Amylases; C001803:tempol