title: Photocatalytically Renewable Micro-electrochemical Sensor for Real-Time Monitoring of Cells.
abstract: Electrode fouling and passivation is a substantial and inevitable limitation in electrochemical biosensing, and it is a great challenge to efficiently remove the contaminant without changing the surface structure and electrochemical performance. Herein, we propose a versatile and efficient strategy based on photocatalytic cleaning to construct renewable electrochemical sensors for cell analysis. This kind of sensor was fabricated by controllable assembly of reduced graphene oxide (RGO) and TiO2 to form a sandwiching RGO@TiO2 structure, followed by deposition of Au nanoparticles (NPs) onto the RGO shell. The Au NPs-RGO composite shell provides high electrochemical performance. Meanwhile, the encapsulated TiO2 ensures an excellent photocatalytic cleaning property. Application of this renewable microsensor for detection of nitric oxide (NO) release from cells demonstrates the great potential of this strategy in electrode regeneration and biosensing.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Jia-Quan
authors.initials: JQ
authors.lastname: Xu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Duo
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China). whhuang@whu.edu.cn.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D055664:Electrochemical Techniques / Q000295:instrumentation*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000032:analysis
chemical_list: D009569:Nitric Oxide
keywords: electrochemistry; nitric oxide; photocatalytic cleaning; recyclable detection; renewable electrochemical sensors