title: [Cardioprotective Effect and Its Mechanism of Total Saponins of Panacis Majoris Rhizoma in Myocardial Infarction Rats].
abstract: OBJECTIVE
  To explore the cardioprotective effect and its mechanism of total saponins of Panacis Majoris Rhizoma in myocardial infarction (MI) rats.
  
  
  METHODS
  The MI model rats induced by ligating anterior descending branch of coronary artery were randomly divided into four group:model group, total saponins of Panacis Majoris Rhizoma (100 and 200 mg/kg) groups and compound Danshen dripping pills group. The rats were orally administrated with drugs once a day for four weeks. Another rats were selected as sham operation group. After four weeks intervention, cardiac function was examined, the serum levels of TNF-α, IL-1β, IL-6 and IL-8 were measured by using ELISA, respectively. The myocardial hypertrophy index was investigated, the myocardial infarct size, degree of ventricular dilatation, myocardial interstitial collagen volume fraction and tissue morphology were investigated by HE, Masson, picric acid-sirius red staining and observing with alight microscope and electron microscope. Protein expressions of phosphorylation IκB-α( pIκB-α) and NF-κB p65 in heart tissue were detected by Western blotting.
  
  
  RESULTS
  Total saponins of Panacis Majoris Rhizoma might significantly decrease the levels of serum TNF-α, IL-1β, IL-6 and IL-8; decrease myocardial hypertrophy indexes, myocardial infarct size, degree of ventricular dilatation and myocardial interstitial collagen volume fraction; improve heart tissue morphology and cardiac function; downregulate protein expression of pIκB-α and NF-κBp65; and upregulate protein expression of SIRT1. The aforementioned action effects of total saponins of Panacis Majoris Rhizoma (200 mg/kg) were similar with compound Danshen dripping pills.
  
  
  CONCLUSIONS
  Total saponins of Panacis Majoris Rhizoma possesses cardioprotective effect against ligating left anterior descending branch induced MI in rats. The mechanism may be related to strengthening SIRT1 expression, inhibiting the phosphorylation of IκB-α, and finally inhibiting the activation of NF-κB and proinflammatory production.
authors.forename: Zhong-wen
authors.initials: ZW
authors.lastname: Bao
authors.forename: Hui-lin
authors.initials: HL
authors.lastname: Qin
authors.forename: Meng-qiong
authors.initials: MQ
authors.lastname: Shi
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Xiao-jiao
authors.initials: XJ
authors.lastname: Yang
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006321:Heart / Q000187:drug effects; D020672:I-kappa B Proteins / Q000378:metabolism; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D009203:Myocardial Infarction / Q000188:drug therapy*; D009206:Myocardium / Q000473:pathology; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B; D005894:Panax / Q000737:chemistry*; D051381:Rats; D027343:Rhizome / Q000737:chemistry; D027561:Salvia miltiorrhiza; D012503:Saponins / Q000737:chemistry*; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; C000606206:Nfkbia protein, rat; C498145:Rela protein, rat; D012503:Saponins; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; C028972:dan-shen root extract