title: Leaf malate and succinate accumulation are out of phase throughout the development of the CAM plant Ananas comosus.
abstract: In plants with Crassulacean Acid Metabolism (CAM), organic acids, mainly malate are crucial intermediates for carbon fixation. In this research we studied the circadian oscillations of three organic anions (malate, citrate, and succinate) in Ananas comosus, assessing the effect of season and plant development stage. Seasonal and plant development dependencies were observed. The circadian oscillations of malate and citrate were typical of CAM pathways reported in the literature. Citrate content was quite stable (25-30 μmol g(-1) FW) along the day, with a seasonal effect. Succinate was shown to have both diurnal and seasonal oscillations and also a correlation with malate, since it accumulated during the afternoon when malate content was normally at a minimum, suggesting a possible mechanistic effect between both anions in CAM and/or respiratory metabolisms.
authors.affiliation: Instituto de Inovação Tecnológica dos Açores (INOVA), Estrada de São Gonçalo, 9504-540 Ponta Delgada, São Miguel, Açores, Portugal; Centro de Ecologia Evolução e Alterações Ambientais (cE3c), Universidade de Lisboa, Faculdade de Ciências, Campo Grande, 1749-016 Lisboa, Portugal. Electronic address: nuno_rainha@sapo.pt.
authors.forename: N
authors.initials: N
authors.lastname: Rainha
authors.affiliation: Instituto de Inovação Tecnológica dos Açores (INOVA), Estrada de São Gonçalo, 9504-540 Ponta Delgada, São Miguel, Açores, Portugal.
authors.forename: V P
authors.initials: VP
authors.lastname: Medeiros
authors.affiliation: Instituto de Inovação Tecnológica dos Açores (INOVA), Estrada de São Gonçalo, 9504-540 Ponta Delgada, São Miguel, Açores, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Ferreira
authors.affiliation: Instituto de Inovação Tecnológica dos Açores (INOVA), Estrada de São Gonçalo, 9504-540 Ponta Delgada, São Miguel, Açores, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Raposo
authors.affiliation: Instituto de Inovação Tecnológica dos Açores (INOVA), Estrada de São Gonçalo, 9504-540 Ponta Delgada, São Miguel, Açores, Portugal.
authors.forename: J P
authors.initials: JP
authors.lastname: Leite
authors.affiliation: Centro de Ecologia Evolução e Alterações Ambientais (cE3c), Universidade de Lisboa, Faculdade de Ciências, Campo Grande, 1749-016 Lisboa, Portugal; Universidade de Lisboa, Faculdade de Ciências, Departamento de Biologia Vegetal, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.affiliation: Instituto Superior de Agronomia, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Pacheco
authors.affiliation: Instituto de Inovação Tecnológica dos Açores (INOVA), Estrada de São Gonçalo, 9504-540 Ponta Delgada, São Miguel, Açores, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Ponte
authors.affiliation: Universidade de Lisboa, Faculdade de Ciências, Departamento de Biologia Vegetal, Campo Grande, 1749-016 Lisboa, Portugal; Biosystems & Integrative Sciences Institute (BioISI), Universidade de Lisboa, Faculdade de Ciências, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: A B
authors.initials: AB
authors.lastname: Silva
mesh_terms: D031230:Ananas / Q000378:metabolism*; D001683:Biological Clocks / Q000502:physiology*; D008293:Malates / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D012621:Seasons; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D008293:Malates; C030298:malic acid; D019802:Succinic Acid
keywords: Ananas comosus; Citrate; Crassulacean Acid Metabolism; Malate; Organic acids; Pineapple; Succinate