title: A High-Throughput Platform for Formulating and Screening Multifunctional Nanoparticles Capable of Simultaneous Delivery of Genes and Transcription Factors.
abstract: Simultaneous delivery of multiple genes and proteins (e.g., transcription factors; TFs) is an emerging issue surrounding therapeutic research due to their ability to regulate cellular circuitry. Current gene and protein delivery strategies, however, are based on slow batch synthesis, which is ineffective, poorly controlled, and incapable of simultaneous delivery of both genes and proteins with synergistic functions. Consequently, advances in this field have been limited to in vitro studies. Here, by integrating microfluidic technologies with a supramolecular synthetic strategy, we present a high-throughput approach for formulating and screening multifunctional supramolecular nanoparticles (MFSNPs) self-assembled from a collection of functional modules to achieve simultaneous delivery of one gene and TF with unprecedented efficiency both in vitro and in vivo. We envision that this new approach could open a new avenue for immunotherapy, stem cell reprogramming, and other therapeutic applications.
authors.affiliation: Laboratory of Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), Chinese Academy of Sciences, No. 11 Beiyitiao, Zhongguancun, Beijing (China).|Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin (China).|California NanoSystems Institute, Department of Chemical and Bimolecular Engineering, UCLA (USA).
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: California NanoSystems Institute, Department of Chemical and Bimolecular Engineering, UCLA (USA).
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Du
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA).
authors.forename: Jin-sil
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA).
authors.forename: Kuan-Ju
authors.initials: KJ
authors.lastname: Chen
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA).
authors.forename: Shuang
authors.initials: S
authors.lastname: Hou
authors.affiliation: California NanoSystems Institute, Department of Chemical and Bimolecular Engineering, UCLA (USA).
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 80708 (Taiwan).
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Lin
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA).
authors.forename: Kevin Sean
authors.initials: KS
authors.lastname: Chen
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA).
authors.forename: Tracy
authors.initials: T
authors.lastname: Ro
authors.affiliation: Division of Liver and Pancreas Transplantation, Department of Surgery, David Geffen School of Medicine, UCLA (USA).
authors.forename: Gerald S
authors.initials: GS
authors.lastname: Lipshutz
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA).
authors.forename: Lily
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin (China).
authors.forename: Linqi
authors.initials: L
authors.lastname: Shi
authors.affiliation: California NanoSystems Institute, Department of Chemical and Bimolecular Engineering, UCLA (USA). luucla@ucla.edu.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Crump Institute for Molecular Imaging, California NanoSystems Institute, Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095 (USA). hrtseng@mednet.ucla.edu.
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
authors.affiliation: Laboratory of Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), Chinese Academy of Sciences, No. 11 Beiyitiao, Zhongguancun, Beijing (China). wanghao@nanoctr.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
mesh_terms: D016503:Drug Delivery Systems; D018014:Gene Transfer Techniques; D005796:Genes; D057166:High-Throughput Screening Assays; D046210:Microfluidic Analytical Techniques; D053758:Nanoparticles / Q000008:administration & dosage* / Q000032:analysis* / Q000737:chemistry; D014157:Transcription Factors / Q000008:administration & dosage*
chemical_list: D014157:Transcription Factors
keywords: biomolecular delivery; microfluidics; nanoparticles; supramolecular chemistry; transcription factors