title: Optimization of medium components for production of chitin deacetylase byBacillus amyloliquefaciensZ7, using response surface methodology.
abstract: Plackett-Burman design and Box-Behnken response surface methodology (RSM) was employed to optimize the medium components for the chitin deacetylase (CDA) activity fromBacillus amyloliquefaciensZ7. Plackett-Burman design was applied to determine the specific medium components affecting CDA activity and found that starch, chitin and MgSO4were critical in augmenting CDA activity. These significant parameters were further optimized using Box-Behnken RSM and the optimum concentrations of starch, chitin and MgSO4were found to be 24.4, 8.8 and 0.19 g/L, respectively. The optimum medium composition was chitin 8.8 g/L, starch 24.4 g/L, yeast extract 10g/L, MgSO40.19 g/L, K2HPO40.3 g/L and NaCl 5 g/L. Under these optimal conditions, the CDA activity ofBacillus amyloliquefaciensZ7 increased distinctly from 18.75 to 27.48 U/mL (46.6% increase in total yield).
authors.affiliation: Key Laboratory for Non-wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China; College of Forestry, Central South University of Forestry and Technology, Changsha, P.R. China.
authors.forename: Yuanhao
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory for Non-wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China; College of Forestry, Central South University of Forestry and Technology, Changsha, P.R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory for Non-wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China; College of Forestry, Central South University of Forestry and Technology, Changsha, P.R. China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Non-wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China; College of Forestry, Central South University of Forestry and Technology, Changsha, P.R. China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Non-wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China; College of Forestry, Central South University of Forestry and Technology, Changsha, P.R. China.
authors.forename: Junang
authors.initials: J
authors.lastname: Liu
keywords: Box–Behnken response surface methodology; Plackett–Burman design; chitin deacetylase