title: Controlled Logic Gates-Switch Gate and Fredkin Gate Based on Enzyme-Biocatalyzed Reactions Realized in Flow Cells.
abstract: Controlled logic gates, where the logic operations on the Data inputs are performed in the way determined by the Control signal, were designed in a chemical fashion. Specifically, the systems where the Data output signals directed to various output channels depending on the logic value of the Control input signal have been designed based on enzyme biocatalyzed reactions performed in a multi-cell flow system. In the Switch gate one Data signal was directed to one of two possible output channels depending on the logic value of the Control input signal. In the reversible Fredkin gate the routing of two Data signals between two output channels is controlled by the third Control signal. The flow devices were created using a network of flow cells, each modified with one enzyme that biocatalyzed one chemical reaction. The enzymatic cascade was realized by moving the solution from one reacting cell to another which were organized in a specific network. The modular design of the enzyme-based systems realized in the flow device allowed easy reconfiguration of the logic system, thus allowing simple extension of the logic operation from the 2-input/3-output channels in the Switch gate to the 3-input/3-output channels in the Fredkin gate. Further increase of the system complexity for realization of various logic processes is feasible with the use of the flow cell modular design.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699, USA.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Fratto
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699, USA. ekatz@clarkson.edu.
authors.forename: Evgeny
authors.identifier: http://orcid.org/0000-0002-1618-4620
authors.initials: E
authors.lastname: Katz
keywords: biocatalysis; biocomputing; enzymes; flow cells; logic gates