title: Silencing of Paralemmin-3 Protects Mice from lipopolysaccharide-induced acute lung injury.
abstract: Excessive inflammatory response induced by lipopolysaccharide (LPS) plays a critical role in the development of acute lung injury (ALI). Paralemmin-3 (PALM3) is a novel protein that can modulate LPS-stimulated inflammatory responses in alveolar epithelial A549 cells. However, it remains unclear whether it is involved in the progression of ALI in vivo. Therefore, we studied the role of PALM3 in the pathogenesis of ALI induced by LPS. ALI was induced by LPS peritoneal injection in C57BL/6J mice. Lentivirus-mediated small interfering RNA (siRNA) targeting the mouse PALM3 gene and a negative control siRNA were intranasally administered to the mice. We found that the expression of PALM3 was up-regulated in the lung tissues obtained from the mouse model of LPS-induced ALI. The LPS-evoked inflammatory response (neutrophils and the concentrations of proinflammatory cytokines [IL-6, IL-1β, TNF-α, MIP-2] in the bronchoalveolar lavage fluid [BALF]), histologic lung injury (lung injury score), permeability of the alveolar capillary barrier (lung wet/dry weight ratio and BALF protein concentration) and mortality rates were attenuated in the PALM3 siRNA-treated mice. These results indicate that PALM3 contributes to the development of ALI in mice challenged with LPS. Inhibiting PALM3 through the intranasal application of specific siRNA protected against LPS-induced ALI.
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Respiratory Medicine, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, Pudong New Area Gongli Hospital, Shanghai, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Field Internal Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Qian
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Lv
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Qian
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Guisheng
authors.initials: G
authors.lastname: Qian
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China. Electronic address: weiyao.weiyao@live.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Respiratory Medicine, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. Electronic address: wuxueling76@126.com.
authors.forename: Xueling
authors.initials: X
authors.lastname: Wu
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology*; D000818:Animals; D055785:Gene Knockdown Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008810:Mice, Inbred C57BL; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D034741:RNA, Small Interfering
keywords: Acute lung injury; Paralemmin-3; lipopolysaccharide