title: Yfiler(®) Plus amplification kit validation and calculation of forensic parameters for two Austrian populations.
abstract: With the new 6-dye AmpFISTR(®) Yfiler(®) Plus amplification kit (Thermo Fisher Scientific, Waltham, MA, USA) a set of 25 Y-chromosomal short tandem repeat loci (Y-STRs), including seven rapidly mutating Y-STRs (RM Y-STRs), is now available for forensic DNA typing. In this study we present our validation data for the AmpFISTR(®) Yfiler(®) Plus amplification kit and show the results of Y-chromosomal typing of 425 unrelated male individuals from two Austrian populations (Salzburg and Upper Austria) with the AmpFISTR(®) Yfiler(®) Plus amplification kit. Forensic parameters were calculated and compared for four Y-STR marker sets. We also typed five brother pairs to evaluate the power of discrimination for related individuals. The AmpFISTR(®) Yfiler(®) Plus (Yfiler Plus) kit appeared to be unimpaired by typical inhibitors such as hematin and humic acid or by large amounts of female components. An upgrade of analyzed markers resulted in increased discrimination capacity that is crucial for forensic trace analysis.
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria.
authors.forename: Ines
authors.initials: I
authors.lastname: Pickrahn
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Müller
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria.
authors.forename: Waltraud
authors.initials: W
authors.lastname: Zahrer
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria.
authors.forename: Bettina
authors.initials: B
authors.lastname: Dunkelmann
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria.
authors.forename: Jan
authors.initials: J
authors.lastname: Cemper-Kiesslich
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Kreindl
authors.affiliation: Department of Legal Medicine, University of Salzburg, Austria. Electronic address: Franz.Neuhuber@sbg.ac.at.
authors.forename: Franz
authors.initials: F
authors.lastname: Neuhuber
mesh_terms: D001317:Austria; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods; D053803:Forensic Genetics / Q000379:methods* / Q000592:standards; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: Haplotype diversity; Rapidly mutating Y-STRs (RM Y-STRs); Y-STRs; Y-chromosome; Yfiler; Yfiler Plus