title: Outer membrane Protein A plays a role in pathogenesis of Acinetobacter nosocomialis.
abstract: Acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of human infections. However, the specific virulence factors of this microorganism have not yet been determined. We investigated the role of outer membrane protein A (OmpA) in the pathogenesis of A. nosocomialis. A ΔompA mutant of the A. nosocomialis ATCC 17903(T) strain was constructed using markerless gene deletion. The ΔompA mutant displayed reduced biofilm formation in polystyrene tubes and reduced adherence to A549 cells in comparison to the wild-type strain. These virulence traits of the ΔompA mutant strain were restored when the ompA gene was complemented. Cytotoxicity was not significantly different between the wild-type strain and the ΔompA mutant when A549 cells were infected with bacteria or treated with outer membrane vesicles (OMVs). However, OMVs from the wild-type strain induced cytotoxicity in HEp-2 cells, whereas OMVs from the ΔompA mutant did not induce cytotoxicity. Proteomic analysis of OMVs revealed that OmpA influenced the distribution of envelope and periplasmic proteins. Overall, this study is the first report that links OmpA to A. nosocomialis pathogenesis, and highlights OmpA as a putative target to develop anti-virulence agents or vaccines against A. nosocomialis infection.
authors.affiliation: a Department of Microbiology , Kyungpook National University School of Medicine , Daegu , Korea.|b Department of Orthopedic Surgery , Ulsan University Hospital, University of Ulsan College of Medicine , Ulsan , Korea.
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: c Department of Nanobiomedical Science , Dankook University , Cheonan , Korea.
authors.forename: Man Hwan
authors.initials: MH
authors.lastname: Oh
authors.affiliation: a Department of Microbiology , Kyungpook National University School of Medicine , Daegu , Korea.
authors.forename: So Hyun
authors.initials: SH
authors.lastname: Jun
authors.affiliation: a Department of Microbiology , Kyungpook National University School of Medicine , Daegu , Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Jeon
authors.affiliation: d Division of Life Science, Korea Basic Science Institute , Daejeon , Korea.
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.affiliation: b Department of Orthopedic Surgery , Ulsan University Hospital, University of Ulsan College of Medicine , Ulsan , Korea.
authors.forename: Kwangho
authors.initials: K
authors.lastname: Kim
authors.affiliation: a Department of Microbiology , Kyungpook National University School of Medicine , Daegu , Korea.
authors.forename: Yoo Chul
authors.initials: YC
authors.lastname: Lee
authors.affiliation: a Department of Microbiology , Kyungpook National University School of Medicine , Daegu , Korea.
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000072283:A549 Cells; D000150:Acinetobacter / Q000235:genetics / Q000472:pathogenicity*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D002460:Cell Line; D002470:Cell Survival; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006801:Humans; D009154:Mutation; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D037521:Virulence Factors; C078167:OMPA outer membrane proteins
keywords: acinetobacter nosocomialis; biofilm; outer membane protein A; outer membrane vesicles; pathogenesis