title: Ratiometric Fluorescent Pattern for Sensing Proteins Using Aqueous Polymer-Pyrene/γ-Cyclodextrin Inclusion Complexes.
abstract: A ratiometric, versatile, and selective fluorescent pattern to sense and distinguish proteins on the basis of dissociation of aqueous polymer-pyrene/γ-cyclodextrin (γ-CD) inclusion complexes was developed. First, two kinds of aqueous polymer-pyrene were prepared via atom transfer radical polymerization using pyrene functionalized initiator. Then, the pyrene molecules could be accumulated into γ-CD cavity and form polymer-pyrene/γ-CD complexes, resulting in appearance of excimer emissions. The resultant complexes responded to proteins in two ways: nonmetalloproteins binding to polymer component triggered dissociation of the inclusion complexes, accompanied by alteration of pyrene excimer/monomer emission and ratiometric fluorescent intensity changes; the presence of metalloproteins could quench pyrene excimer/monomer emission because of energy transfer. Moreover, the fluorescent responses of the inclusion complexes to different proteins could be modulated by changing polymer type and chain length, resulting in a tunable selectivity and sensitivity. The proposed fluorescent inclusion complexes could provide a promising platform for sensing proteins.
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.|Graduate School, University of Chinese Academy of Sciences , Beijing 100049, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D003208:Concanavalin A / Q000032:analysis*; D005453:Fluorescence; D006801:Humans; D015394:Molecular Structure; D009211:Myoglobin / Q000032:analysis*; D010434:Pepsin A / Q000032:analysis*; D011108:Polymers / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D012709:Serum Albumin / Q000032:analysis*; D014867:Water / Q000737:chemistry; D047408:gamma-Cyclodextrins / Q000737:chemistry
chemical_list: D009211:Myoglobin; D011108:Polymers; D011721:Pyrenes; D012709:Serum Albumin; D047408:gamma-Cyclodextrins; D014867:Water; D003208:Concanavalin A; C030984:pyrene; D010434:Pepsin A; C023792:gamma-cyclodextrin