title: Target-Induced and Equipment-Free DNA Amplification with a Simple Paper Device.
abstract: We report on a paper device capable of carrying out target-induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA-enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.
authors.affiliation: Departments of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: Christy Y
authors.initials: CY
authors.lastname: Hui
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Departments of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Gu
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: Balamurali
authors.initials: B
authors.lastname: Kannan
authors.affiliation: Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.
authors.forename: Sana
authors.initials: S
authors.lastname: Jahanshahi-Anbuhi
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.|Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.
authors.forename: Carlos D M
authors.initials: CD
authors.lastname: Filipe
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada. brennanj@mcmaster.ca.
authors.forename: John D
authors.initials: JD
authors.lastname: Brennan
authors.affiliation: Departments of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada. liying@mcmaster.ca.|Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada. liying@mcmaster.ca.
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Li
mesh_terms: D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D010209:Paper
chemical_list: D004247:DNA
keywords: biosensor; paper; point of care diagnostics; pullulan; rolling circle amplification