title: Adipose-derived stem cells seeded on polyglycolic acid for the treatment of stress urinary incontinence.
abstract: OBJECTIVE
  To evaluate the effect and possible mechanism of suburethral tissue-engineered sling in an animal model of stress urinary incontinence (SUI).
  
  
  METHODS
  Adipose-derived stem cells (ADSCs) were obtained from the adipose tissues of rats. The differentiation potential, proliferation, and viability of rat ADSCs were evaluated after infecting these cells with a lentiviral vector-encoding green fluorescent protein (GFP). Next, GFP transfected ADSCs were seeded on polyglycolic acid (PGA) fibers to construct the tissue-engineered sling with the induction of 5-azacytidine (5-Aza). Afterward, the tissue-engineered slings were transplanted into a rat model of SUI that was established by vaginal balloon dilatation method and bilateral ovariectomy. Histology and the leak point pressure measurements were performed at 2 months after tissue-engineered sling implantation.
  
  
  RESULTS
  The ADSCs were found to be efficiently transfected with GFP, without any effects on proliferation, cell cycle and multi-differentiation potential. After been seeded on PGA fibers, ADSCs formed tissue-engineered slings in 4 weeks of induction culture. Two months after implantation, the mean leak point pressure (LPP) was significantly increased in sling-treated rats compared with the balloon-injured ovariectomized rats. Immunofluorescence assay showed that some of the GFP expressing cells stained positive for muscle-specific markers.
  
  
  CONCLUSIONS
  The newly suburethral tissue-engineered sling restores LPP in the rat model of SUI, which could be an effective treatment in future SUI therapy.
authors.affiliation: Department of Urology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200240, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200240, People's Republic of China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Shi
authors.affiliation: Department of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Yi Shan Road 600, Shanghai, 200233, People's Republic of China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Yi Shan Road 600, Shanghai, 200233, People's Republic of China.
authors.forename: Nai-Long
authors.initials: NL
authors.lastname: Cao
authors.affiliation: Department of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Yi Shan Road 600, Shanghai, 200233, People's Republic of China. jamesqfu@aliyun.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D011100:Polyglycolic Acid / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D053825:Suburethral Slings; D023822:Tissue Engineering / Q000379:methods*; D014550:Urinary Incontinence, Stress / Q000628:therapy*
chemical_list: D011100:Polyglycolic Acid
keywords: Adipose-derived stem cells (ADSCs); Polyglycolic acid (PGA); Sling; Stress urinary incontinence (SUI)