title: Possible Involvement of the Inhibition of NF-κB Factor in Anti-Inflammatory Actions That Melatonin Exerts on Mast Cells.
abstract: Melatonin is a molecule endogenously produced in a wide variety of immune cells, including mast cells (RBL-2H3). It exhibits immunomodulatory, anti-inflammatory and anti-apoptotic properties. The physiologic mechanisms underlying these activities of melatonin have not been clarified in mast cells. This work is designed to determine the anti-inflammatory effect and mechanism of action of melatonin on activated mast cells. RBL-2H3 were pre-treated with exogenous melatonin (MELx) at physiological (100nM) and pharmacological (1 mM) doses for 30 min, washed and activated with PMACI (phorbol 12-myristate 13-acetate plus calcium ionophore A23187) for 2 h and 12 h. The data shows that pre-treatment of MELx in stimulated mast cells, significantly reduced the levels of endogenous melatonin production (MELn), TNF-α and IL-6. These effects are directly related with the MELx concentration used. MELx also inhibited IKK/NF-κB signal transduction pathway in stimulated mast cells. These results indicate a molecular basis for the ability of melatonin to prevent inflammation and for the treatment of allergic inflammatory diseases through the down-regulation of mast cell activation. J. Cell. Biochem. 117: 1926-1933, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Medical Biochemistry, Molecular Biology and Immunology, University of Seville Medical School, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Maldonado
authors.affiliation: Department of Medical Biochemistry, Molecular Biology and Immunology, University of Seville Medical School, Spain.
authors.forename: H
authors.initials: H
authors.lastname: García-Moreno
authors.affiliation: Department of Medical Biochemistry, Molecular Biology and Immunology, University of Seville Medical School, Spain.
authors.forename: C
authors.initials: C
authors.lastname: González-Yanes
authors.affiliation: Department of Medical Biochemistry, Molecular Biology and Immunology, University of Seville Medical School, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: Calvo
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000001:Calcimycin / Q000633:toxicity; D045744:Cell Line, Tumor; D015850:Interleukin-6 / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D008550:Melatonin / Q000494:pharmacology*; D016328:NF-kappa B / Q000276:immunology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D008550:Melatonin; D013755:Tetradecanoylphorbol Acetate
keywords: CYTOKINES; INFLAMMATION; MELATONIN; NF-κB; RBL-2H3 MAST CELLS