title: Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing.
abstract: A novel protocol of label-free electrochemical impedance immunosensor based on bovine serum albumin-nitidine chloride-reduced graphene oxide (BSA-NC-rGO) nanocomposite was proposed for quantitative determination of carcino-embryonic antigen (CEA). BSA was anchored to rGO via the aromatic plane of NC by π-stacking interaction to realize bio-functionalization of rGO, and then gold nanoparticles (AuNPs) were electrodeposited onto the surface of BSA-NC-rGO nanocomposite. The morphology, conductivity and interaction of different nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and UV-vis spectrum. CEA monoclonal antibody (anti-CEA) was conjugated to AuNPs via gold-thiol chemistry to construct electrochemical immunosensing platform, and the specific immunoreaction between CEA and anti-CEA was monitored by EIS. Under optimum conditions, CEA could be quantified in a wide range of 0.1-200 ng mL(-1) (R=0.9948) with low detection limit of 0.067 ng mL(-1). The proposed immunosensor exhibited great potential for detecting blood samples.
authors.affiliation: College of Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Deng
authors.affiliation: College of Sciences, Shanghai University, Shanghai 200444, PR China. Electronic address: luck@shu.edu.cn.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Surgery, Shanghai Xinhua Hospital, Shanghai Jiaotong University, Shanghai 200092, PR China. Electronic address: hanbaosan@126.com.
authors.forename: Baosan
authors.initials: B
authors.lastname: Han
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D053119:Benzophenanthridines / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood*; D002417:Cattle; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D053119:Benzophenanthridines; D002272:Carcinoembryonic Antigen; D010087:Oxides; D012710:Serum Albumin, Bovine; D006046:Gold; D006108:Graphite; C013615:nitidine
keywords: Bovine serum albumin; Gold nanoparticles; Impedimetric immunosensor; Nitidine chloride; Reduced graphene oxide