title: Bioaccumulation of nickel by E. sativa and role of plant growth promoting rhizobacteria (PGPRs) under nickel stress.
abstract: Phytoremediation potential of plants can be enhanced in association with microbes. Further, many plant growth-promoting rhizobacteria can improve growth under stress. The present study was conducted to investigate the effect of Pseudomonas putida (P. putida) on nickel (Ni) uptake and on growth of Eruca sativa (E. sativa). Three different levels of Ni (low; 150 ug/g, medium; 250 ug/g and high; 500 ug/g) were applied to the soil containing E. sativa seedlings, with or without P. putida. Ni-toxicity was measured by metamorphic parameters including shoot length, root length, biomass, chlorophyll and proline and Ni contents. Inoculation with P. putida increased 34% and 41% in root and shoot length and 38% and 24% in fresh, dry weight respectively, as compared to non-inoculated plants. Similarly, Ni uptake increased by up to 46% following P. putida inoculation as compared to non-inoculated plants. Indole acetic acid, siderophore and 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity in the growing media enhanced growth and Ni uptake in E. sativa. The present results offer insight on Plant Growth Promoting Rhizobacteria (PGPR), such as P. putida, for the potential to enhance the plant growth by inhibiting the adverse effects of Ni in E. sativa.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: Kamran_2093@yahoo.com.
authors.forename: Muhammad Aqeel
authors.initials: MA
authors.lastname: Kamran
authors.affiliation: Department of Biosciences, COMSATS Institute of Information and Technology, Islamabad, Pakistan.
authors.forename: Syed Ali Musstjab Akber Shah
authors.initials: SAMAS
authors.lastname: Eqani
authors.affiliation: University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Sadia
authors.initials: S
authors.lastname: Bibi
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, PR China.
authors.forename: Ren-Kou
authors.initials: RK
authors.lastname: Xu
authors.affiliation: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.lastname: Amna
authors.affiliation: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Muhammad Farooq Hussain
authors.initials: MFH
authors.lastname: Monis
authors.affiliation: Norwegian Institute for Air Research, FRAM - High North Research, Centre on Climate and the Environment, NO-9296 Tromsø, Norway.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Katsoyiannis
authors.affiliation: Department of Biosciences, COMSATS Institute of Information and Technology, Islamabad, Pakistan.
authors.forename: Habib
authors.initials: H
authors.lastname: Bokhari
authors.affiliation: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: hassaan_javed@yahoo.com.
authors.forename: Hassan Javed
authors.initials: HJ
authors.lastname: Chaudhary
mesh_terms: D001673:Biodegradation, Environmental; D019607:Brassicaceae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D009532:Nickel / Q000378:metabolism* / Q000633:toxicity; D018517:Plant Roots / Q000254:growth & development; D016958:Pseudomonas putida / Q000502:physiology*; D036226:Seedlings / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological
chemical_list: D012987:Soil; D009532:Nickel
keywords: E. sativa; Nickel; Phyto remediation; Phytoremediation; Plant growth