title: G-lignin and hemicellulosic monosaccharides distinctively affect biomass digestibility in rapeseed.
abstract: In this study, total 19 straw samples from four Brassica species were determined with a diverse cell wall composition and varied biomass enzymatic digestibility under sulfuric acid or lime pretreatment. Correlation analysis was then performed to detect effects of cell wall compositions and wall polymer features (cellulose crystallinity, hemicellulosic monosaccharides and lignin monomers) on rapeseeds biomass digestibility. As a result, coniferyl alcohol (G-lignin) showed a strongly negative effect on biomass saccharification, whereas hemicellulosic monosaccharides (fucose, galactose, arabinose and rhamnose) were positive factors on lignocellulose digestions. Notably, chemical analyses of four typical pairs of samples indicated that hemicellulosic monosaccharides and G-lignin may coordinately influence biomass digestibility in rapeseeds. In addition, Brassica napus with lower lignin content exhibited more efficiency on both biomass enzymatic saccharification and ethanol production, compared with Brassica junjea. Hence, this study has at first time provided a genetic strategy on cell wall modification towards bioenergy rapeseed breeding.
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Pei
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Youbing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology & Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Changbing
authors.initials: C
authors.lastname: Yu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Tingdong
authors.initials: T
authors.lastname: Fu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Peng
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: chenpeng@mail.hzau.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
mesh_terms: D056804:Biofuels; D018533:Biomass; D029741:Brassica rapa / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D002482:Cellulose / Q000032:analysis; D003246:Conservation of Energy Resources; D000431:Ethanol / Q000378:metabolism; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D009005:Monosaccharides / Q000737:chemistry* / Q000378:metabolism; D010087:Oxides / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D056804:Biofuels; D017610:Calcium Compounds; D009005:Monosaccharides; D010087:Oxides; D013464:Sulfuric Acids; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C016538:lime; C033158:sulfuric acid
keywords: Biomass digestibility; Enzymatic saccharification; G-lignin; Hemicellulosic monosaccharide; Rapeseed