title: Expression of pro-angiogenic genes in mesenchymal stem cells derived from dermis of patients with psoriasis.
abstract: BACKGROUND
  Recent experimental studies revealed that angiogenesis and lymphangiogenesis are closely related to psoriasis. Our microarray analysis suggested that the pro-angiogenic genes platelet endothelial cell adhesion molecule-1 (PECAM1), facio-genital dysplasia-5 (FGD5), prostaglandin-endoperoxide synthase-1 (PTGS1), melanoma cell adhesion molecule (MCAM), vasohibin-2 (VASH2), and stabilin-1 (STAB1) are differentially expressed in dermal mesenchymal stem cells in psoriasis.
  
  
  OBJECTIVE
  The aim of this study was to investigate the mRNA and protein expression of PECAM1, FGD5, PTGS1, MCAM, VASH2, and STAB1 for angiogenesis and the possible mechanisms in psoriasis.
  
  
  METHODS
  We studied 12 patients with plaque psoriasis and 14 healthy controls matched for age and sex. Dermal mesenchymal stem cells were expanded, passaged, and identified by cellular morphology, immunophenotyping, and multipotential differentiation. The mRNA and protein expression of the above-mentioned six genes were confirmed by quantitative real-time reverse transcription-polymerase chain reaction and Western blotting.
  
  
  RESULTS
  The significantly decreased expression of PECAM1, PTGS1, FGD5, and MCAM at both mRNA and protein level (except VASH2 and STAB1) were demonstrated in mesenchymal stem cells from psoriatic skin lesions compared with non-lesional from healthy controls.
  
  
  CONCLUSIONS
  We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM rather than VASH2 and STAB1 may be play a vital role in pathological dermal angiogenesis disorders of psoriasis. Therefore, anti-angiogenesis is attractive and offers future potential for application in patients with psoriasis.
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Xuping
authors.initials: X
authors.lastname: Niu
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: WenJuan
authors.initials: W
authors.lastname: Chang
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Junqin
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory Animal Center, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000328:Adult; D042501:Angiogenic Proteins / Q000235:genetics / Q000378:metabolism; D051929:CD146 Antigen / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016201:Receptors, Lymphocyte Homing / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D042501:Angiogenic Proteins; D051929:CD146 Antigen; D015816:Cell Adhesion Molecules, Neuronal; C572072:FGD5 protein, human; D020662:Guanine Nucleotide Exchange Factors; C497902:MCAM protein, human; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D016201:Receptors, Lymphocyte Homing; C449926:STAB1 protein, human; C574858:VASH2 protein, human; D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human