title: Paired comparison of methylene blue- and amotosalen-treated plasma and cryoprecipitate.
abstract: OBJECTIVE
  Cryoprecipitate is used in the treatment of patients with acquired hypofibrinogenaemia. Studies have not directly compared cryoprecipitate produced following pathogen inactivation (PI) of fresh-frozen plasma (FFP) using different systems. The effects of methylene blue (MB) and amotosalen (AS) PI systems on the quality of FFP and cryoprecipitate were investigated in a paired study.
  
  
  METHODS
  Seven group A and 7 group O pools of plasma were prepared and split into individual units and rapidly frozen to produce FFP. Units of FFP were thawed and either PI treated with MB or amotosalen, or left untreated (control). Samples of FFP along with the corresponding cryoprecipitate were tested for a range of coagulation factors, thrombin generation (TGT) and rotational thromboelastometry (ROTEM).
  
  
  RESULTS
  AS-FFP showed a smaller decrease following treatment for most coagulation factors analysed than MB-FFP, except fibrinogen (antigen) and factor VII, partly due to lower volume losses. There was no significant difference between treatment methods for fibrinogen content of cryoprecipitate with treated units meeting current UK specification, or TGT and ROTEM parameters studied.
  
  
  CONCLUSIONS
  MB-cryo contained a significantly higher content of FVIII and lower content of FXIII when compared to AS-cryo, with no difference in fibrinogen activity.
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.
authors.forename: L
authors.initials: L
authors.lastname: Backholer
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.
authors.forename: M
authors.initials: M
authors.lastname: Wiltshire
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.
authors.forename: S
authors.initials: S
authors.lastname: Proffitt
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.
authors.forename: P
authors.initials: P
authors.lastname: Cookson
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Cardigan
mesh_terms: D001779:Blood Coagulation Factors / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D016555:Matched-Pair Analysis; D008751:Methylene Blue / Q000494:pharmacology*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010949:Plasma / Q000737:chemistry / Q000821:virology; D013916:Thrombelastography; D013917:Thrombin / Q000032:analysis / Q000378:metabolism; D038301:Virus Inactivation / Q000187:drug effects
chemical_list: D001779:Blood Coagulation Factors; D011564:Furocoumarins; D017319:Photosensitizing Agents; D005340:Fibrinogen; D013917:Thrombin; C118577:amotosalen; D008751:Methylene Blue
keywords: cryoprecipitate; fresh-frozen plasma; pathogen inactivation