title: A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to α-glucosidase inhibitor screening.
abstract: The medicines targeted at α-glucosidase played an important role in anti-diabetes and anti-HIV therapy. Unfortunately, the method based on fluorescent assay strategy for α-glucosidase inhibitor screening remains poorly investigated. In this study, a novel "Turn On" fluorescence sensor platform has been developed for trace α-glucosidase inhibitor screening from natural medicines. Firstly, carbon dots were prepared by one-pot synthesis and used as the signal output. Combining with the carbon dots, cobalt oxyhydroxide (CoOOH) nanoflakes were employed to build the fluorescence resonance energy transfer (FRET) based sensor platform. Secondly, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was innovatively introduced as α-glucosidase substrate. With hydrolysis of AAG by α-glucosidase, ascorbic acids (AA) were released that can rapidly reduce CoOOH nanoflakes to Co(2+), and then FRET was stopped accompanying with the fluorescence recovery of CDs. The sensor platform was ultrasensitive to AA with a detection limit of 5 nM, ensuring the sensitive monitoring of enzyme activity. Acarbose was used as the inhibitor model and its inhibition rate is proportional to the logarithm of concentration in range of 10(-9)-10(-3)M with the correlation coefficient of R(2)=0.996, and an ultralow limit of detection of ~1×10(-9)M was obtained. The inhibiting ability of seven compounds isolated from natural medicines was also evaluated. The constructed sensor platform was proven to be sensitive and selective as well as cost-effective, facile and reliable, making it promising as a candidate for trace α-glucosidase inhibitor screening.
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China; Key Laboratories of Chemical Safety and Health, China National Center for Food Safety Risk Assessment, Beijing 100050, China. Electronic address: 61254368@163.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Weiheng
authors.initials: W
authors.lastname: Kong
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Shuaimin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Peiwei
authors.initials: P
authors.lastname: Gong
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xia
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China; Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. Electronic address: jmyou6304@163.com.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
authors.affiliation: Key Laboratories of Chemical Safety and Health, China National Center for Food Safety Risk Assessment, Beijing 100050, China. Electronic address: wuyongning@cfsa.net.cn.
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
mesh_terms: D020909:Acarbose / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis / Q000378:metabolism; D002244:Carbon / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009584:Nitrogen / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D010087:Oxides; C477250:HCoO2; D003035:Cobalt; D002244:Carbon; D000520:alpha-Glucosidases; D009584:Nitrogen; D001205:Ascorbic Acid; D020909:Acarbose
keywords: Enzyme inhibition; Fluorescence resonance energy transfer; Nitrogen-doped carbon dots; α-Glucosidase