title: Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules.
abstract: Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Ksenia
authors.initials: K
authors.lastname: Krupina
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Kleiss
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Metzger
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Sadek
authors.initials: S
authors.lastname: Fournane
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Schmucker
authors.affiliation: Institute for Genetics, University of Cologne, 50674 Cologne, Germany.
authors.forename: Kay
authors.initials: K
authors.lastname: Hofmann
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Fischer
authors.affiliation: Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, 67085 Strasbourg, France.
authors.forename: Nicodeme
authors.initials: N
authors.lastname: Paul
authors.affiliation: Centre for Gene Regulation & Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Iain Malcolm
authors.initials: IM
authors.lastname: Porter
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France; Computer Science Department, ICube, UMR 7357, University of Strasbourg, CNRS, Fédération de Médecine Translationnelle, 67085 Strasbourg, France.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Raffelsberger
authors.affiliation: Computer Science Department, ICube, UMR 7357, University of Strasbourg, CNRS, Fédération de Médecine Translationnelle, 67085 Strasbourg, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Poch
authors.affiliation: Centre for Gene Regulation & Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Jason Reese
authors.initials: JR
authors.lastname: Swedlow
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Brino
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France. Electronic address: sumara@igbmc.fr.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sumara
mesh_terms: D000705:Anaphase / Q000502:physiology; D064107:Aurora Kinase B / Q000378:metabolism*; D002460:Cell Line; D020090:Chromosome Segregation / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*; D054875:Ubiquitination / Q000502:physiology
chemical_list: D025801:Ubiquitin; D064107:Aurora Kinase B; D017027:Protein Tyrosine Phosphatases; C489397:UBASH3B protein, human
keywords: Aurora B; UBASH3B; chromosome segregation; mitosis; ubiquitin; ubiquitin receptor