title: Simultaneous saccharification and fermentation of dilute alkaline-pretreated corn stover for enhanced butanol production by Clostridium saccharobutylicum DSM 13864.
abstract: Simultaneous saccharification and fermentation (SSF) process was applied for biobutanol production by Clostridium saccharobutylicum DSM 13864 from corn stover (CS). The key influential factors in SSF process, including corn steep liquor concentration, dry biomass and enzyme loading, SSF temperature, inoculation size and pre-hydrolysis time were optimized. In 5-L bioreactor with SSF process, butanol titer and productivity of 12.3 g/L and 0.257 g/L/h were achieved at 48 h, which were 20.6% and 21.2% higher than those in separate hydrolysis and fermentation (SHF), respectively. The butanol yield reached 0.175 g/g pretreated CS in SSF, representing 50.9% increase than that in SHF (0.116 g/g pretreated CS). This study proves the feasibility of efficient and economic production of biobutanol from CS by SSF.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Dong
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Ji-Cai
authors.initials: JC
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Guo-Chao
authors.initials: GC
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Rui-Zhi
authors.initials: RZ
authors.lastname: Han
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China yni@jiangnan.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Ni
mesh_terms: D000468:Alkalies; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000191:economics / Q000379:methods; D000440:Butanols / Q000032:analysis / Q000191:economics / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D003313:Zea mays / Q000378:metabolism*
chemical_list: D000468:Alkalies; D000440:Butanols
keywords: Clostridium saccharobutylicum; butanol; corn stover; simultaneous saccharification and fermentation