title: Equol Effectively Inhibits Toxic Activity of Human Neutrophils without Influencing Their Viability.
abstract: Equol (7,4'-dihydroxy-isoflavan, or 4',7-isoflavandiol) is a chroman derivative produced by intestinal bacteria in response to soy isoflavone intake in some, but not in all, humans. Equol shows strong anti-oxidant, anti-estrogenic, anti-cancerous and anti-inflammatory properties. The antioxidative capacity of equol has recently received considerable attention, and it has been used for preventing and treating several diseases. We investigated the effect of equol on human neutrophils, extra- and intracellular formation of oxidants, the phosphorylation of protein regulating NADPH oxidase and its effect on apoptosis. Neutrophils, isolated from blood from healthy subjects, were tested upon activation with various stimulants, proper for reactive oxygen species (ROS) production and treated by equol. Equol has the ability to reduce the toxic action of neutrophils. With increasing concentrations, equol decreased the amount of oxidants produced by neutrophils both extra- and intracellularly. The phosphorylation of p40(phox) (a component of NADPH oxidase, responsible for the assembly of functional oxidase in intracellular membranes) was reduced in the presence of equol. The experiments showed that equol did not change the number of viable, apoptotic or dead neutrophils significantly in all concentrations used. These results indicate the promising effect of equol in the operation of ROS in different mechanisms in the model of inflammation.
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dx00FA;bravskx00E1; cesta 9, Bratislava, Slovak Republic.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pažoureková
authors.forename: Marianna
authors.initials: M
authors.lastname: Lucová
authors.forename: Radomír
authors.initials: R
authors.lastname: Nosál
authors.forename: Katarína
authors.initials: K
authors.lastname: Drábiková
authors.forename: Juraj
authors.initials: J
authors.lastname: Harmatha
authors.forename: Ján
authors.initials: J
authors.lastname: Šmidrkal
authors.forename: Viera
authors.initials: V
authors.lastname: Jančinová
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D060754:Equol / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D060754:Equol; D019255:NADPH Oxidases; C113333:NCF4 protein, human; D013755:Tetradecanoylphorbol Acetate