title: Rosmarinic Acid Attenuates Cell Damage against UVB Radiation-Induced Oxidative Stress via Enhancing Antioxidant Effects in Human HaCaT Cells.
abstract: This study was designed to investigate the cytoprotective effect of rosmarinic acid (RA) on ultraviolet B (UVB)-induced oxidative stress in HaCaT keratinocytes. RA exerted a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. RA also attenuated UVB-induced oxidative macromolecular damage, including protein carbonyl content, DNA strand breaks, and the level of 8-isoprostane. Furthermore, RA increased the expression and activity of superoxide dismutase, catalase, heme oxygenase-1, and their transcription factor Nrf2, which are decreased by UVB radiation. Collectively, these data indicate that RA can provide substantial cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and has potential to be developed as a medical agent for ROS-induced skin diseases.
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Pattage Madushan Dilhara Jayatissa
authors.initials: PM
authors.lastname: Fernando
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Yea Seong
authors.initials: YS
authors.lastname: Ryu
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Susara Ruwan Kumara Madduma
authors.initials: SR
authors.lastname: Hewage
authors.affiliation: Aging Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors.forename: Sung Wook
authors.initials: SW
authors.lastname: Chae
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
keywords: Antioxidant system; Oxidative stress; Reactive oxygen species; Rosmarinic acid; Ultraviolet B