title: Mice housed on coal dust-contaminated sand: A model to evaluate the impacts of coal mining on health.
abstract: Coal dust is the most important air pollutant in coal mining in regards to producing deleterious health effects. It permeates the surrounding environment threatening public health. The aim of this study was to evaluate the toxic effects associated with exposure to sand contaminated with coal dust particles below 38 μm in diameter, obtained from a mineral sample collected in the largest coal mine in South America, La Loma, Cesar, Colombia. Sterilized sand was spiked with coal dust to obtain concentrations ranging from zero to 4% coal dust. To model natural exposure, mice were housed for eight weeks in boxes containing this mixture as bedding after which, they were euthanized and blood and tissue samples were collected. Real time PCR analysis revealed an increase in Cyp1A1 mRNA for living on sand with coal dust concentrations greater than 2% compared to mice living on sand without coal dust. Unexpectedly, for mice on coal dust-polluted sand, Sod1, Scd1 and Nqo1 hepatic mRNA were downregulated. The Comet assay in peripheral blood cells and the micronucleus test in blood smears, showed a significant potential genotoxic effect only at the highest coal dust concentration. Histopathological analysis revealed vascular congestion and peribronchial inflammation in the lungs. A dose-response relationship for the presence of hepatic steatosis, vacuolization and nuclei enlargements was observed in the exposed animals. The data suggest living on a soil polluted with coal dust induces molecular, cellular and histopathological changes in mice. Accordingly, the proposed model can be used to identify deleterious effects of exposure to coal dust deposited in soils that may pose health risks for surrounding wildlife populations.
authors.affiliation: Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena, 130014, Colombia. Electronic address: kcaballerog@unicartagena.edu.co.
authors.forename: Karina
authors.initials: K
authors.lastname: Caballero-Gallardo
authors.affiliation: Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena, 130014, Colombia. Electronic address: joliverov@unicartagena.edu.co.
authors.forename: Jesus
authors.initials: J
authors.lastname: Olivero-Verbel
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003031:Coal / Q000633:toxicity*; D003032:Coal Mining; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004391:Dust; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008111:Liver Function Tests; D051379:Mice; D008813:Mice, Inbred ICR; D009152:Mutagenicity Tests; D016273:Occupational Exposure / Q000009:adverse effects*; D016272:Occupational Health; D010316:Particle Size; D012822:Silicon Dioxide
chemical_list: D003031:Coal; D004391:Dust; D012822:Silicon Dioxide; D019363:Cytochrome P-450 CYP1A1
keywords: Colombia; Liver; Mining; Total suspended particles; Toxicity