title: Feed additive production by fermentation of herb Polygonum hydropiper L. and cassava pulp with simultaneous flavonoid dissolution.
abstract: Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett-Burman factorial design (PB design) was used to optimize the flavonoid dissolution and protein content. Using the screening function of PB design, four different significant factors for the two response variables were acquired: factors A (CP) and B (PHL) for the flavonoid dissolution versus factors G (inoculum size) and H (fermentation time) for protein content. Then, two RSMs were used sequentially to improve the values of the two response variables separately. The mutual corroboration of the experimental results in the present study confirmed the validity of the associated experimental design. The validation experiment showed a flavonoid dissolution rate of 94.00%, and a protein content of 18.20%, gaining an increase in 21.20% and 199.10% over the control, respectively. The present study confirms the feasibility of feed additive production by Saccharomyces cerevisiae with CP and PHL and simultaneous optimization of flavonoid dissolution and protein content using a two-stage RSM.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou, People's Republic of China.
authors.forename: Zhen-Tao
authors.initials: ZT
authors.lastname: Song
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou, People's Republic of China.|Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, People's Republic of China.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Zhu
mesh_terms: D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005419:Flavonoids / Q000737:chemistry*; D005503:Food Additives / Q000138:chemical synthesis* / Q000737:chemistry; D002365:Manihot / Q000737:chemistry / Q000378:metabolism; D028502:Polygonum / Q000737:chemistry / Q000378:metabolism; D012995:Solubility
chemical_list: D003470:Culture Media; D005419:Flavonoids; D005503:Food Additives; D000431:Ethanol
keywords: Polygonum hydropiper L; cassava pulp; flavonoid dissolution; response surface methodology; simultaneous saccharification and fermentation