title: Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes.
abstract: The anti-obesity effects of starter (Leuconostoc mesenteroides+Lactobacillus plantarum) fermented kimchi on 3T3-L1 adipocyte were studied using naturally fermented kimchi (NK), a functional kimchi (FK, NK supplemented with green tea), and FK supplemented with added starters (FKS). Oil red O staining and cellular levels of triglyceride (TG) and glycerol were used to evaluate the in vitro anti-obesity effects of these kimchis in 3T3-L1 cells. The expressions of adipogenesis/lipogenesis-related genes of peroxisome proliferator-active receptor (PPAR)-γ, CCAAT/enhance-binding protein (C/EBP)-α, and fatty acid synthase (FAS) were determined by RT-PCR. Kimchis, especially FKS, markedly decreased TG levels and increased levels of intracellular glycerol and lipid lipolysis. In addition, FKS also reduced the mRNA levels of PPAR-γ, C/EBP-α, and FAS, which are related to adipogenesis/lipogenesis in 3T3-L1 cells. These results suggest the anti-obesity effects of FKS were to due to enhanced lipolysis and reduced adipogenesis/lipogenesis in 3T3-L1 adipocytes.
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busan 46241, Korea.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busan 46241, Korea; Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi 541004, People's Republic of China.
authors.forename: Jia-Le
authors.initials: JL
authors.lastname: Song
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busan 46241, Korea.
authors.forename: Eui-Seong
authors.initials: ES
authors.lastname: Park
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busan 46241, Korea.
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Ju
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busan 46241, Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busan 46241, Korea.
authors.forename: Kun-Young
authors.initials: KY
authors.lastname: Park
keywords: 3T3-L1 cells; antiobesity; kimchi; starters