title: Expressions of lysophosphatidic acid receptors in the development of human ovarian carcinoma.
abstract: OBJECTIVE
  To investigate the associations between the expressions of three lysophosphatidic acid (LPA) receptors (LPA1-3) and the development of ovarian carcinoma (OC).
  
  
  METHODS
  Ovarian tissue specimens, including normal ovarian epithelium tissues, benign ovarian tumor tissues and OC tissues were collected from patients who underwent surgical resections between March 2012 and December 2014. Immunohistochemical staining was used to detect LPA receptor expressions in ovarian tissues. Reverse transcription-polymerase chain reaction and Western blotting were used to detect mRNA and protein expression of LPA receptors, respectively. Association analysis between LPA receptors protein expression and clinical pathological characteristics was conducted. The value of LPA2 and LPA3 in discriminating OC was confirmed by receiver-operator characteristic (ROC) curves analysis.
  
  
  RESULTS
  The positive expression rates of LPA2 and LPA3 in OC group was obviously higher than normal control and benign groups. The LPA2 and LPA3 mRNA and protein levels in OC group were higher than in normal control and benign groups. LPA2 and LPA3 mRNA expression levels were positively correlated with LPA2 and LPA3 protein expression in OC group. ROC curve analysis revealed that LPA2 yield a specificity of 96.3% and a sensitivity of 97.9%, and LPA3 yield a specificity of 98.5% and a sensitivity of 97.9% for the detection of OC.
  
  
  CONCLUSIONS
  LPA2 and LPA3 were highly expressed in OC tissues, which may be involved in the development of OC. Further, LPA2 and LPA3 had higher sensitivity and specificity in distinguishing the OC from benign ovarian tumors, which could be potential diagnostic indictors in OC.
authors.affiliation: Department of Gynecology and Obstetrics, Zhujiang Hospital of Southern Medical University Guangzhou 510515, P. R. China.
authors.forename: Jinge
authors.initials: J
authors.lastname: Si
authors.affiliation: Department of Gynecology and Obstetrics, Zhongshan Affiliated Hospital of Sun Yat-Sen University Zhongshan 528403, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Gynecology and Obstetrics, Zhujiang Hospital of Southern Medical University Guangzhou 510515, P. R. China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Gynecology, Boai Hospital of Zhongshan Zhongshan 528403, P. R. China.
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Yan
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xiamen University Xiamen 361003, P. R. China.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Tang
keywords: Ovarian carcinoma; ROC curve analysis; immunohistochemical staining; lysophosphatidic acid receptors; pathological grades; pathological stages; pathological types; reverse transcription-polymerase chain reaction