title: Mobility-Selected Ion Trapping and Enrichment Using Structures for Lossless Ion Manipulations.
abstract: The integration of ion mobility spectrometry (IMS) with mass spectrometry (MS) and the ability to trap ions in IMS-MS measurements is of great importance for performing reactions, accumulating ions, and increasing analytical measurement sensitivity. The development of Structures for Lossless Ion Manipulations (SLIM) offers the potential for ion manipulations in an extended and more effective manner, while opening opportunities for many more complex sequences of manipulations. Here, we demonstrate an ion separation and trapping module and a method based upon SLIM that consists of a linear mobility ion drift region, a switch/tee and a trapping region that allows the isolation and accumulation of mobility-separated species. The operation and optimization of the SLIM switch/tee and trap are described and demonstrated for the enrichment of the low abundance ions. A linear improvement in ion intensity was observed with the number of trapping/accumulation events using the SLIM trap, illustrating its potential for enhancing the sensitivity of low abundance or targeted species.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Tsung-Chi
authors.initials: TC
authors.lastname: Chen
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Yehia M
authors.initials: YM
authors.lastname: Ibrahim
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Ian K
authors.initials: IK
authors.lastname: Webb
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Sandilya V B
authors.initials: SV
authors.lastname: Garimella
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Hamid
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Liulin
authors.initials: L
authors.lastname: Deng
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: William E
authors.initials: WE
authors.lastname: Karnesky
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Spencer A
authors.initials: SA
authors.lastname: Prost
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Sandoval
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Randolph V
authors.initials: RV
authors.lastname: Norheim
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Tolmachev
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352, United States.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D006801:Humans; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D011487:Protein Conformation
chemical_list: D007477:Ions; D010455:Peptides