title: Biochanin A Ameliorates Arsenic-Induced Hepato- and Hematotoxicity in Rats.
abstract: Biochanin A (BCA) is a natural organic compound of the phytoestrogenic isoflavone class that has antioxidant and metal chelator properties in the presence of transition metal ions, however, its efficacy in animal models is still obscure. Therefore, the objective of this study was to investigate the protective effects of BCA against arsenic-induced hepatic injury and hematotoxicity in rats. The results suggest that arsenic intoxicated rats showed significantly higher levels of plasma hepatic markers than normal control rats. Furthermore, an increase in lipid peroxidation with depletion of reduced glutathione (GSH) and activities of superoxide dismutase (SOD) and catalase (CAT) occurred in the livers of rats exposed to arsenic. Administration of BCA (20 mg/kg·bw/day) and selenium (3 mg/kg·bw/day) resulted in a significant reversal of hepatic and oxidative stress markers in arsenic-intoxicated rats. A low dose of BCA (10 mg/kg·bw/day) did not show any preventive effect, while a high dose of BCA (40 mg/kg·bw/day) partially prevented all hepatotoxicity events. These biochemical perturbations were supported by histopathological observations of the liver. Our results suggest that administration of BCA (20 mg/kg·bw/day) attenuated the arsenic hepatotoxicity, a property that could contribute to the therapeutic approaches for chronic liver diseases.
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. bisnanojalal@gmail.com.
authors.forename: Abdulkadhar Mohamed
authors.initials: AM
authors.lastname: Jalaludeen
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. hows4u@nate.com.
authors.forename: Woo Tae
authors.initials: WT
authors.lastname: Ha
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. ranran24@kku.ac.kr.
authors.forename: Ran
authors.initials: R
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. jhkim541@konkuk.ac.kr.
authors.forename: Jin Hoi
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. dojt@konkuk.ac.kr.
authors.forename: Jeong Tae
authors.initials: JT
authors.lastname: Do
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. chankyu@konkuk.ac.kr.
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. urbangypsy@hanmail.net.
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Heo
authors.affiliation: Division of Food Bioscience, College of Biomedical and Health Science, Konkuk University, Chung-ju 380-701, Korea. abseng@kku.ac.kr.
authors.forename: Won Young
authors.initials: WY
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. songh@konkuk.ac.kr.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001151:Arsenic / Q000633:toxicity; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D004909:Erythrocyte Indices / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D017382:Reactive Oxygen Species; D019833:Genistein; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium; D001151:Arsenic; C004541:biochanin A
keywords: arsenic; biochanin A; lipid peroxidation; reactive oxygen species; selenium