title: Protective effect of hydrogen sulfide on renal injury in the experimental unilateral ureteral obstruction.
abstract: OBJECTIVE
  Ureteral obstruction is a common pathology and causes kidney fibrosis and dysfunction at late period. In this present study, we investigated the antifibrotic and antiinflammatory effects of hydrogen sulfide on kidney damage after unilateral ureteral obstruction (UUO) in rats.
  
  
  METHODS
  24 rats were divided into four groups. Group 1 was control, group 2 was sham, group 3 included rats with UUO and group 4 rats with UUO which were given sodium hydrogen sulfide (NaHS)-exogenous donor of hydrogen sulfide (intraperitoneally 56 µmoL/kg/day). After 14 days, rats were killed and their kidneys were taken and blood analysis was performed. Tubular necrosis, mononuclear cell infiltration and interstitial fibrosis were determined histopathologically in a part of the kidneys; nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH) levels were determined in the other part of the kidneys. Urea-creatinine levels were investigated by blood analysis. Statistical analyses were made by the Chi-square test and one-way analysis of variance (ANOVA).
  
  
  RESULTS
  There was no significantly difference for urea-creatinine levels among groups. Pathologically, there was serious tubular necrosis and fibrosis in group 3 and there was significantly decreasing of tubular necrosis and fibrosis in group 4 (p<0.005). Also, there was significantly increase of NO and MDA levels and decrease of GSH levels in group 3 compared to other groups (p<0.005).
  
  
  CONCLUSIONS
  hydrogen sulfide prevents kidney damage with antioxidant and antiinflammatory effect.
authors.affiliation: Department of Urology, Bahcelievler State Hospital, Istanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Dursun
authors.affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Otunctemur
authors.affiliation: Department of Urology, Katip Celebi University, Ataturk Training and Research Hospital, Izmir, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Ozbek
authors.affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Sahin
authors.affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Besiroglu
authors.affiliation: Department of Biochemistry, Kocaeli University, Kocaeli, Turkey.
authors.forename: Ozgur Doga
authors.initials: OD
authors.lastname: Ozsoy
authors.affiliation: Department of Biochemistry, Kocaeli University, Kocaeli, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Cekmen
authors.affiliation: Department of Pathology, Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Somay
authors.affiliation: Department of Pathology, Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey.
authors.forename: Nurver
authors.initials: N
authors.lastname: Ozbay
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D005355:Fibrosis; D005978:Glutathione / Q000032:analysis; D006862:Hydrogen Sulfide / Q000494:pharmacology* / Q000627:therapeutic use; D007668:Kidney / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis; D018384:Oxidative Stress; D011897:Random Allocation; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D015203:Reproducibility of Results; D013997:Time Factors; D014508:Urea / Q000097:blood; D014517:Ureteral Obstruction / Q000150:complications / Q000517:prevention & control*
chemical_list: D000893:Anti-Inflammatory Agents; D009569:Nitric Oxide; D008315:Malondialdehyde; D014508:Urea; D003404:Creatinine; D005978:Glutathione; D006862:Hydrogen Sulfide