title: Effective pretreatment of dilute NaOH-soaked chestnut shell with glycerol-HClO4-water media: structural characterization, enzymatic saccharification, and ethanol fermentation.
abstract: In this study, an effective pretreatment of dilute NaOH-soaked chestnut shell (CNS) with glycerol-HClO4-water (88.8:1.2:10, w/w/w) media at 130 °C for 30 min was successfully demonstrated. Results revealed that the combination pretreatment removed 66.0 % of lignin and 73.7 % of hemicellulose in untreated CNS. The changes in the structural features (crystallinity, morphology, and porosity) of the solid residue of CNS were characterized with Fourier transform infrared spectroscopy, fluorescent microscope, scanning electron microscopy, and X-ray diffraction. Biotransformation of glycerol-HClO4-water pretreated-NaOH-soaked CNS (50 g/L) with a cocktail of enzymes for 72 h, the reducing sugars and glucose were 39.7 and 33.4 g/L, respectively. Moreover, the recovered hydrolyzates containing 20 g/L glucose had no inhibitory effects on the ethanol-fermenting microorganism, and the ethanol production was 0.45 g/g glucose within 48 h. In conclusion, this combination pretreatment shows promise as pretreatment solvent for wheat straw, although the in-depth exploration of this subject is needed.
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China. heyucai2001@163.com.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Di
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Da-Zhou
authors.initials: DZ
authors.lastname: Gao
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Gang-Gang
authors.initials: GG
authors.lastname: Chong
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Mei-Zi
authors.initials: MZ
authors.lastname: Huang
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
mesh_terms: D000431:Ethanol / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D031324:Juglans / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry; D010472:Perchlorates / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D010472:Perchlorates; D011134:Polysaccharides; D014867:Water; D000431:Ethanol; D012972:Sodium Hydroxide; C007916:hemicellulose; D008031:Lignin; D005947:Glucose; D005990:Glycerol; C494474:perchlorate
keywords: Chestnut shell; Glycerol; NaOH soaking; Pretreatment; Saccharification