title: Improving cytotoxicity against cancer cells by chemo-photodynamic combined modalities using silver-graphene quantum dots nanocomposites.
abstract: The combination of chemotherapy and photodynamic therapy has emerged as a promising strategy for cancer therapy due to its synergistic effects. In this work, PEGylated silver nanoparticles decorated with graphene quantum dots (Ag-GQDs) were tested as a platform to deliver a chemotherapy drug and a photosensitizer, simultaneously, in chemo-photodynamic therapy against HeLa and DU145 cancer cells in vitro. Ag-GQDs have displayed high efficiency in delivering doxorubicin as a model chemotherapy drug to both cancer cells. The Ag-GQDs exhibited a strong antitumor activity by inducing apoptosis in cancer cells without affecting the viability of normal cells. Moreover, the Ag-GQDs exhibited a cytotoxic effect due to the generation of the reactive singlet oxygen upon 425 nm irradiation, indicating their applicability in photodynamic therapy. In comparison with chemo or photodynamic treatment alone, the combined treatment of Ag-GQDs conjugated with doxorubicin under irradiation with a 425 nm lamp significantly increased the death in DU145 and HeLa. This study suggests Ag-GQDs as a multifunctional and efficient therapeutic system for chemo-photodynamic modalities in cancer therapy.
authors.affiliation: Department of Physics, University of Puerto Rico - Rio Piedras Campus, San Juan, PR, USA; Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA.
authors.forename: Khaled
authors.initials: K
authors.lastname: Habiba
authors.affiliation: Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA; Department of Biology, University of Puerto Rico - Rio Piedras Campus, San Juan, PR, USA.
authors.forename: Joel
authors.initials: J
authors.lastname: Encarnacion-Rosado
authors.affiliation: Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA; Faculty of Education, University of Puerto Rico - Rio Piedras Campus, San Juan, PR, USA.
authors.forename: Kenny
authors.initials: K
authors.lastname: Garcia-Pabon
authors.affiliation: Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA; Department of Biology, University of Puerto Rico - Bayamon Campus, Bayamon, PR, USA.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Villalobos-Santos
authors.affiliation: Department of Physics, University of Puerto Rico - Rio Piedras Campus, San Juan, PR, USA.
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Makarov
authors.affiliation: Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA; Department of Physics, University of Puerto Rico - Bayamon Campus, Bayamon, PR, USA.
authors.forename: Javier A
authors.initials: JA
authors.lastname: Avalos
authors.affiliation: Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA; Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, PR, USA; Department of Chemistry, University of Puerto Rico - Rio Piedras Campus, San Juan, PR, USA.
authors.forename: Brad R
authors.initials: BR
authors.lastname: Weiner
authors.affiliation: Department of Physics, University of Puerto Rico - Rio Piedras Campus, San Juan, PR, USA; Molecular Sciences Research Center, University of Puerto Rico, San Juan, PR, USA; Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, PR, USA.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Morell
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D003131:Combined Modality Therapy / Q000379:methods; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004359:Drug Therapy, Combination; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology; D045663:Quantum Dots / Q000627:therapeutic use; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D026082:Singlet Oxygen / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D012834:Silver; D006108:Graphite; D004317:Doxorubicin
keywords: cancer therapy; drug delivery; multifunctional nanoparticles; nanocomposites; photodynamic therapy; silver nanoparticles