title: Effect and mechanism of dihydroartemisinin on proliferation, metastasis and apoptosis of human osteosarcoma cells.
abstract: Osteosarcoma represents an aggressive type of bone malignancy that poses a significant health threat. The objective of the current study was to analyze the effect and mechanism of dihydroartemisinin (DHA) on the proliferation, metastasis and apoptosis of human osteosarcoma cells. A gradient concentration of DHA (15, 25 and 35 μmol.L-1) was used to stimulate the cells, along with control and Dimethyl sulfoxide (DMSO). The phenotypic outcomes were characterized using MTT assay, clone formation assay, Hoechst 33258 staining assay, luciferase reporter plasmid assay, Western blot and wound healing assay. In addition, IBM SPSS Statistics 18.0 software was applied for statistical analysis and all experimental data were expressed as mean ± s.d. Analysis of variance (ANOVA) was applied to compare the differences among multiple groups. Our results demonstrated that DHA inhibited the proliferation and metastasis of osteosarcoma cells and promoted the apoptosis in the cytomorphosis.
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: C
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Traditional Chinese Orthopedics and Physiotherapy, Traditional Chinese Medicine Hospital of Baoji, Baoji, Shaanxi, China.
authors.forename: P Y
authors.initials: PY
authors.lastname: Ao
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Luo
authors.affiliation: Department of Orthopedics, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Radiology, Peoples Hospital of Zhengzhou University and Henan Province Peoples Hospital, Zhengzhou, Henan, China.
authors.forename: D P
authors.initials: DP
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D037621:Artemisinins / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D012516:Osteosarcoma / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary
chemical_list: D037621:Artemisinins; C039060:artenimol