title: Effects of acupuncturing Pishu combined with Ginsenoside Rg3 on the immune function of rats with chronic fatigue.
abstract: OBJECTIVE
  This study was designed to investigate the effects of acupuncturing Pishu combined with Ginsenoside Rg3 on the immune function of rats with chronic fatigue.
  
  
  METHODS
  Forty male SD rats were equally randomized into control group, chronic fatigue system group (CFS), Ginsenoside Rg3 (Rg3) group, acupuncture group and acupuncture combined with Ginsenoside Rg3 (A+Rg3) group. Rats with chronic fatigue were established by bounding and forced swimming in cold water once daily for 21 days except control group, then the rats in the acupuncture and A+Rg3 group were treated by manual acupuncture stimulation of bilateral "Pishu" once daily for 7 days. Ginsenoside Rg3 was administered by intravenous to the rats of the A+Rg3 and Rg3 group for 7 days in dosages of 2 mg/kg body weight, and two markers of physical fatigue were evaluated: body weight and blood lactic acid (LA). The percentages of CD3(+) lymphocytes, CD4(+) lymphocytes, and CD8(+) lymphocytes in the spleens of the rats were evaluated using flow cytometric analysis. Serum IFN-gamma (IFN-γ) and IL-4 contents were detected by ELISA.
  
  
  RESULTS
  Increased body weight and reduced blood LA concentrations were found in the rat of Rg3 group and A+Rg3 group than that in CFS group. The rat of Rg3 group and A+Rg3 group also showed a significant increase in the percentage of CD4(+) lymphocytes and a significant decrease in the percentage of CD8(+) lymphocytes and correct CD4(+)/CD8(+) ratio. Compared with the CFS group, the level of IFN-γ in the Rg3, acupuncture and A+Rg3 groups was reduced and IL-4 was increased.
  
  
  CONCLUSIONS
  Acupuncture and Rg3 can improve the immune system activity of CFS rats and acupuncturing Pishu combined with Rg3 was significantly superior compared with Rg3 and acupuncture, respectively.
authors.affiliation: Liaoning University of Traditional Chinese Medicine Shenyang 110032, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Liaoning University of Traditional Chinese Medicine Shenyang 110032, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Liaoning University of Traditional Chinese Medicine Shenyang 110032, China.
authors.forename: Xiande
authors.initials: X
authors.lastname: Ma
authors.affiliation: Liaoning University of Traditional Chinese Medicine Shenyang 110032, China.
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Chen
keywords: Forced swimming test; Ginsenoside Rg3; acupuncture; fatigue; systemic immune response