title: [Genetic diversity of the Rhizobia and screening of high-efficient growth-promoting strains isolated from Sesbania cannabina in Rudong County].
abstract: OBJECTIVE
  To study the genetic diversity and phylogeny of Rhizobia isolated from Sesbania cannabina growing on the tidal flat in Rudong County and screen high-efficient growth-promoting strains as Rhizobia inoculator to S. cannabina.
  
  
  METHODS
  Phylogenetic analyses were based on 16S rRNA gene, housekeeping genes (recA, atpD, glnII) and symbiotic genes (nodA, nifH). The growth-promoting efficiency was tested by plant inoculation assay on S. cannabina in greenhouse.
  
  
  RESULTS
  The 32 isolates belonged to Ensifer, Neorhizobium, Rhizobium, and most closely related to E. meliloti, N. huautlense, R. pusense. The phylogenies of nodA and nifH were congruent, and most closely related to E. saheli. The 7 representative isolates were resistant to high concentration of NaCl (5%, W/V), and YIC5082 grew well in TY medium with 6% NaCl. In plant inoculation assay, all the 7 representative isolates were effective on symbiotic nitrogen fixation, and 6 out of the 7 isolates significantly enhanced the fresh weight and height of plants.
  
  
  CONCLUSIONS
  Rhizobia isolated from S. cannabina growing on the tidal flat in Rudong County showed rich genetic diversity. N. huautlense and E. meliloti were the dominant species. Most of the isolates showed fine growth-promoting efficiency and salt tolerance. YIC5077 showed the best growth-promoting efficiency, good nodulation and nitrogen fixation abilities, which has promising potential applications as Rhizobia inoculator to S. cannabina.
authors.forename: Xiangyue
authors.initials: X
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.forename: Hailong
authors.initials: H
authors.lastname: Wu
authors.forename: Wanping
authors.initials: W
authors.lastname: Song
authors.forename: Hualing
authors.initials: H
authors.lastname: Xu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012230:Rhizobiaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D049931:Sesbania / Q000254:growth & development* / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012965:Sodium Chloride