title: Ectopic expression of R3 MYB transcription factor gene OsTCL1 in Arabidopsis, but not rice, affects trichome and root hair formation.
abstract: In Arabidopsis, a MYB-bHLH-WD40 (MBW) transcriptional activator complex activates the homeodomain protein gene GLABRA2 (GL2), leading to the promotion of trichome formation and inhibition of root hair formation. The same MBW complex also activates single-repeat R3 MYB genes. R3 MYBs in turn, play a negative feedback role by competing with R2R3 MYB proteins for binding bHLH proteins, thus blocking the formation of the MBW complex. By BLASTing the rice (Oryza sativa) protein database using the entire amino acid sequence of Arabidopsis R3 MYB transcription factor TRICHOMELESS1 (TCL1), we found that there are two genes in rice genome encoding R3 MYB transcription factors, namely Oryza sativa TRICHOMELESS1 (OsTCL1) and OsTCL2. Expressing OsTCL1 in Arabidopsis inhibited trichome formation and promoted root hair formation, and OsTCL1 interacted with GL3 when tested in Arabidopsis protoplasts. Consistent with these observations, expression levels of GL2, R2R3 MYB transcription factor gene GLABRA1 (GL1) and several R3 MYB genes were greatly reduced, indicating that OsTCL1 is functional R3 MYB. However, trichome and root hair formation in transgenic rice plants overexpressing OsTCL1 remained largely unchanged, and elevated expression of OsGL2 was observed in the transgenic rice plants, indicating that rice may use different mechanisms to regulate trichome formation.
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Kaijie
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Hainan
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Qingnan
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Ling
authors.initials: L
authors.lastname: Cai
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Xutong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Epigenetics of MOE &Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Shucai
authors.initials: S
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics*; D000066630:Ectopic Gene Expression; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D056426:Genetic Loci; D042541:Intracellular Space / Q000378:metabolism; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D011485:Protein Binding; D021381:Protein Transport; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D064201:Trichomes / Q000235:genetics* / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors