title: [Rapid determination of lactose, sucrose, glucose and fructose in foods by capillary zone electrophoresis with indirect ultraviolet detection].
abstract: A new and rapid method for the simultaneous determination of lactose, sucrose, glucose and fructose by capillary zone electrophoresis ( CZE) with indirect ultraviolet detection was developed. The separation was completed with an uncoated fused-silica capillary with 30.2 cm of total length (effective length of 20 cm) x 50 µm. The separation buffer consisted of 4 mmol/L potassium sorbate, 10 mmol/L sodium phosphate, 30 mmol/L NaOH (pH 12. 56) and 0. 5 mmol/L hexadecytrimethylammonium bromide (CTAB). The separation was performed at a voltage of -8 kV with the ultraviolet detection at 254 nm. The analysis of the four carbohydrates was completed within 10 min. The limits of detection (S/N= 3) for lactose, sucrose, glucose and fructose were 50, 75, 25 and 25 mg/L, and the limits of quantification (S/N = 10) were 150, 225, 75 and 75 mg/L, respectively. The average recoveries for the four carbohydrates were in the range of 87.0%-107.0% with the relative standard deviations of 1.2%-4.7%. No organic solvent was consumed throughout the whole process of the analysis. The method was used for the analysis of nine food samples and a quality control sample. The results demonstrated that the method is simple, rapid, accurate, and suitable for the routine analysis of the four carbohydrates in food samples.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ding
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002021:Buffers; D019075:Electrophoresis, Capillary; D005504:Food Analysis / Q000379:methods*; D005632:Fructose / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D007785:Lactose / Q000032:analysis*; D013395:Sucrose / Q000032:analysis*
chemical_list: D002021:Buffers; D005632:Fructose; D013395:Sucrose; D005947:Glucose; D007785:Lactose