title: IDENTIFICATION OF PROTEASOME ALPHA6 SUBUNIT ASSOCIATED WITH DELTAMETHRIN RESISTANCE IN Drosophila melanogaster Kc CELLS.
abstract: Differential expression of the proteasome alpha6 (prosalpha6) was previously reported between Plutella xylostella strains that are resistant or susceptible to the pesticide deltamethrin (DM). This finding indicated that the prosalpha6 may be involved in DM resistance. In this article, qPCR analysis revealed that the prosalpha6 was also significantly upregulated in Drosophila Kc cells treated with DM. To better understand the contribution of prosalpha6 in DM resistance, RNA interference, heterologous expression, and a proteasome inhibitor (MG-132) were used. MG-132 was used to suppress proteasomal activity, and the dsRNA was designed to block the function of prosalpha6. The results indicated that both MG-132 and prosalpha6 knockdown decreased the cellular viability following DM treatment. Prosalpha6 was cloned and transfected into Drosophila Kc cells. The result showed that overexpression of prosalpha6 in Drosophila Kc cells conferred some protection against DM. Taken together, our results indicate that prosalpha6 is involved in Drosophila cells DM resistance.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Energy and Mechanical Engineering, Nanjing Normal University, Nanjing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Chi
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agriculture Science, Guiyang, China.
authors.forename: Fengliang
authors.initials: F
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
authors.forename: Luogen
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D004331:Drosophila melanogaster / Q000378:metabolism*; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D007305:Insecticide Resistance; D009570:Nitriles; D011722:Pyrethrins; D015854:Up-Regulation
chemical_list: D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D010450:Endopeptidases; C000611097:proteasome alpha6 subunit, Drosophila
keywords: MG-132; RNA interference; deltamethrin; overexpression; pesticide resistance; prosalpha6