title: Photodynamic detection of canine mammary gland tumours after oral administration of 5-aminolevulinic acid.
abstract: 5-Aminolevulinic acid (5-ALA) is widely used in photodynamic detection (PDD) and therapy. We evaluated the pharmacokinetics of 5-ALA-induced porphyrins and its effectiveness in PDD in dogs with mammary gland tumours (MGTs) following oral administration. Healthy dogs and those with MGTs (nine each) were orally administered 4 mg kg-15-ALA. Protoporphyrin IX (PpIX) was not detected in the plasma of healthy dogs but it peaked in dogs with MGT at 2 h after 5-ALA administration. In the PDD study, 16 dogs with MGT were orally administered 40 mg kg-15-ALA, and MGT but not normal tissue showed red fluorescence after 2-4 h. Photon counts were 6635-63 890 and 59-4011 (median, 19 943 and 919) for MGT and non-tumour tissues, respectively. Cell density strongly correlated with PpIX photon counts of MGT tissue of the dogs (R = 0.743, P = 0.0009). We suggest that 5-ALA-PDD might be an effective diagnostic tool for MGTs.
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yokoe
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ogura
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishizuka
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Bio- and Material Photonics, Chitose Institute of Science and Technology, Chitose, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Sugiyama
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Murahata
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ito
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000284:Administration, Oral; D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000175:diagnosis*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000494:pharmacology*; D011166:Porphyrins / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000662:veterinary*
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D000622:Aminolevulinic Acid
keywords: 5-aminolevulinic acid; dog; mammary gland tumour; protoporphyrin IX