title: Over-expression of Arabidopsis thaliana SFD1/GLY1, the gene encoding plastid localized glycerol-3-phosphate dehydrogenase, increases plastidic lipid content in transgenic rice plants.
abstract: Lipids are the major constituents of all membranous structures in plants. Plants possess two pathways for lipid biosynthesis: the prokaryotic pathway (i.e., plastidic pathway) and the eukaryotic pathway (i.e., endoplasmic-reticulum (ER) pathway). Whereas some plants synthesize galactolipids from diacylglycerol assembled in the plastid, others, including rice, derive their galactolipids from diacylglycerols assembled by the eukaryotic pathway. Arabidopsis thaliana glycerol-3-phosphate dehydrogenase (G3pDH), coded by SUPPRESSOR OF FATTY ACID DESATURASE 1 (SFD1; alias GLY1) gene, catalyzes the formation of glycerol 3-phosphate (G3p), the backbone of many membrane lipids. Here SFD1 was introduced to rice as a transgene. Arabidopsis SFD1 localizes in rice plastids and its over-expression increases plastidic membrane lipid content in transgenic rice plants without any major impact on ER lipids. The results suggest that over-expression of plastidic G3pDH enhances biosynthesis of plastid-localized lipids in rice. Lipid composition in the transgenic plants is consistent with increased phosphatidylglycerol synthesis in the plastid and increased galactolipid synthesis from diacylglycerol produced via the ER pathway. The transgenic plants show a higher photosynthetic assimilation rate, suggesting a possible application of this finding in crop improvement.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 415, New Delhi 110067, India.
authors.forename: Vijayata
authors.initials: V
authors.lastname: Singh
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 415, New Delhi 110067, India.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 415, New Delhi 110067, India.
authors.forename: Adnan
authors.initials: A
authors.lastname: Siddiqui
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 415, New Delhi 110067, India.
authors.forename: Subaran
authors.initials: S
authors.lastname: Singh
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 415, New Delhi 110067, India.
authors.forename: Zeeshan Zahoor
authors.initials: ZZ
authors.lastname: Banday
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, 415, New Delhi 110067, India.
authors.forename: Ashis Kumar
authors.initials: AK
authors.lastname: Nandi
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D038983:Galactolipids / Q000378:metabolism*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D018087:Plastids / Q000201:enzymology; D011993:Recombinant Fusion Proteins; D036226:Seedlings / Q000166:cytology / Q000201:enzymology / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D005227:Fatty Acids; D038983:Galactolipids; D005994:Glycerophosphates; D008055:Lipids; D011993:Recombinant Fusion Proteins; C029620:alpha-glycerophosphoric acid; D005993:Glycerolphosphate Dehydrogenase; C487187:SFD1 protein, Arabidopsis
keywords: Arabidopsis; ESI–MS/MS; G3pDH; Lipids; SFD1; Transgenic rice