title: MiR-125b Functions as a Tumor Suppressor and Enhances Chemosensitivity to Cisplatin in Osteosarcoma.
abstract: MicroRNAs are highly conserved noncoding RNA that negatively modulate protein expression at a posttranscriptional and/or translational level and are deeply involved in the pathogenesis of several types of cancers. To date, the potential microRNAs regulating the growth and progression of osteosarcoma are not fully identified yet. Previous reports have shown differentially expressed miR-125b in osteosarcoma. However, the role of miR-125b in human osteosarcoma has not been totally illuminated. In this study, we have shown that miR-125b was downregulated in human osteosarcoma tissues compared to the adjacent tissues and effects as a tumor suppressor in vitro We found that stable overexpression of miR-125b in osteosarcoma cell lines U2OS and MG-63 inhibited cell proliferation, migration, and invasion. Our data also verified that Bcl-2 is the target of miR-125b. Meanwhile, we showed that Bcl-2 was inversely correlated with miR-125b in osteosarcoma tissues. More importantly, we proved that miR-125b increased the chemosensitivity of osteosarcoma cell lines to cisplatin by targeting Bcl-2. In conclusion, our data demonstrate that miR-125b is a tumor suppressor and support its potential application for the treatment of osteosarcoma in the future.
authors.affiliation: Department of Trauma and Orthopedics, Institute of Chinese People's Liberation Army, General Hospital of Jinan Military Command, Jinan, China wangfeicz@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Trauma and Orthopedics, Institute of Chinese People's Liberation Army, General Hospital of Jinan Military Command, Jinan, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Yu
authors.affiliation: Oncology Department, Feicheng Mining Central Hospital, Taian, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Clinical Auditory Center, Eye & Ear Infirmary of Shandong Provincial Hospital Group, Shandong Provincial Key Laboratory of Otology, Jinan, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Trauma and Orthopedics, Institute of Chinese People's Liberation Army, General Hospital of Jinan Military Command, Jinan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Emergency, Traffic Hospital of Shandong Province, Jinan, China.
authors.forename: Haiyin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Trauma and Orthopedics, Institute of Chinese People's Liberation Army, General Hospital of Jinan Military Command, Jinan, China doctorzhaotingbao@163.com.
authors.forename: Tingbao
authors.initials: T
authors.lastname: Zhao
mesh_terms: D001859:Bone Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002945:Cisplatin / Q000494:pharmacology*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D012516:Osteosarcoma / Q000188:drug therapy* / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D002945:Cisplatin
keywords: Bcl-2; cisplatin; invasion; miR-125b; osteosarcoma; proliferation