title: Effect of arsenate As (V) on the biomarkers of Myriophyllum alterniflorum in oligotrophic and eutrophic conditions.
abstract: Alternate watermilfoil, Myriophyllum alterniflorum is an aquatic macrophyte found in the Limousin rivers (France) whose potential for biomonitoring of metal pollution has been demonstrated. The objective of the present study carried out in vitro was to identify biomarkers for an early detection of the pollution by a metalloid As (V) in eutrophic and oligotrophic conditions. A synthetic medium of similar composition to the waters of the River Vienne was prepared. The morphological development of watermilfoil was monitored for 30 days, with or without contamination by 100 μg L(-1) As (V). In addition, the mineralization of plants and the analysis of biomarkers (chlorophylls, photosynthetic and respiratory intensities …) were investigated after 21 days. Our results indicated that eutrophic medium, induced a decrease in chlorophyll pigments, in growth and an increase in H2O2 compared to the oligotrophic medium. While, the presence of As (V), led to a decrease in the osmotic potential, pigment content, photosynthesis and respiration rates and an inhibition of shoot branching of plants in both conditions. However, a significant increase in H2O2 content was noted in the eutrophic medium. Finally, As (V) was found to be more accumulated in roots than shoots in both conditions but was more accumulated in oligotrophic one. Therefore, we can conclude that the water trophic level modifies the response of M. alterniflorum in presence of arsenate. Thus, M. alterniflorum shows a great promise in water-quality biomonitoring.
authors.affiliation: Lebanese University, Plateform for Research and Analysis in Environnemental Sciences, Doctoral School of Sciences and Technologies, P.O.Box 5, Hadath, Beirut, Lebanon; Limoges University, Faculty of Pharmacy, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, Laboratoire de Botanique et Cryptogamie, 2, rue Dr. Marcland, 87025 Limoges, France; Limoges University, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, 123, Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: M
authors.initials: M
authors.lastname: Krayem
authors.affiliation: Limoges University, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, 123, Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: V
authors.initials: V
authors.lastname: Deluchat
authors.affiliation: Limoges University, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, 123, Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: M
authors.initials: M
authors.lastname: Rabiet
authors.affiliation: Limoges University, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, 123, Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: K
authors.initials: K
authors.lastname: Cleries
authors.affiliation: Limoges University, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, 123, Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Lenain
authors.affiliation: Lebanese University, Plateform for Research and Analysis in Environnemental Sciences, Doctoral School of Sciences and Technologies, P.O.Box 5, Hadath, Beirut, Lebanon.
authors.forename: Z
authors.initials: Z
authors.lastname: Saad
authors.affiliation: Lebanese University, Plateform for Research and Analysis in Environnemental Sciences, Doctoral School of Sciences and Technologies, P.O.Box 5, Hadath, Beirut, Lebanon.
authors.forename: V
authors.initials: V
authors.lastname: Kazpard
authors.affiliation: Limoges University, Faculty of Pharmacy, Groupement de Recherche Eau, Sol, Environnement - GRESE EA 4330, Laboratoire de Botanique et Cryptogamie, 2, rue Dr. Marcland, 87025 Limoges, France. Electronic address: pascal.labrousse@unilim.fr.
authors.forename: P
authors.initials: P
authors.lastname: Labrousse
mesh_terms: D001149:Arsenates / Q000493:pharmacokinetics / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004784:Environmental Monitoring; D005602:France; D006861:Hydrogen Peroxide / Q000378:metabolism; D019684:Magnoliopsida / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D045483:Rivers; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D001149:Arsenates; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D006861:Hydrogen Peroxide
keywords: Arsenate; Biomarkers; Myriophyllum alterniflorum; Phosphates; Trophic level