title: [Protective Effect of Lycium ruthenicum on Peripheral Blood System Against Radiation Injury in Mice].
abstract: OBJECTIVE
  To study the protective effect of Lycium ruthenicum on peripheral blood system against radiation injury in mice.
  
  
  METHODS
  Kunming mice were randomly divided into control group, model group, positive group and Lycium ruthenicum high dose (8 g/kg), middle dose (4 g/kg) and low dose (2 g/kg)treatment groups that experimented three days after irradiation. In the same way, groups were set at 7 days and 14 days after irradiation respectively. Lycium ruthenicum extract were administered orally to the mice in the three Lycium ruthenicum treatment groups and normal saline were administered orally to the mice in control group and model group for 14 days. Positive group were treated with radioprotective agent amifostine (WR-2721) at 30 min before irradiation. Except control group, mice in other groups received quantity of 5 Gy X-radiation whole body evenly with one time. Hemogram, organ index, DNA, Caspase-3, Caspase-6 and P53 contents were observed at the 3rd, 7th and 14th day after irradiation.
  
  
  RESULTS
  Lycium ruthenicum significantly increased the total red blood cell count, hemoglobin count, the indexes of spleen and thymus and bone marrow DNA contents (P < 0.05), as well as decreased Caspase-3 and Caspase-6 contents in serum and the expression of P53 in intestinal crypt epithelial cells.
  
  
  CONCLUSIONS
  The results showed that Lycium ruthenicum had protective effects on peripheral blood system against radiation injury in mice.
authors.forename: Ya-bin
authors.initials: YB
authors.lastname: Duan
authors.forename: Xing-cheng
authors.initials: XC
authors.lastname: Yao
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: Chen
authors.forename: Jun-bo
authors.initials: JB
authors.lastname: Zhu
authors.forename: Juan-ling
authors.initials: JL
authors.lastname: Zhang
authors.forename: Xiang-yang
authors.initials: XY
authors.lastname: Li
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004906:Erythrocyte Count; D032305:Lycium / Q000737:chemistry*; D051379:Mice; D011832:Radiation Injuries / Q000188:drug therapy*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects; D014965:X-Rays
chemical_list: D004365:Drugs, Chinese Herbal; D011837:Radiation-Protective Agents; C505409:Casp3 protein, mouse; D053148:Caspase 3