title: Advances in the integration of optical and mass spectrometry molecular imaging technologies: from omics data to molecular signature discovery.
abstract: Many molecular mechanisms in complex biological processes of diseases cannot be fully understood without direct visualization. In the last decades, advances in imaging principles and technologies have expanded our ability to capture and analyze the morphology of tissues and cells' components on conventional widefield optical and fluorescence microscopies and their derivatives confocal and multiphoton fluorescence laser-scanning microscopes, as well as transmission electron microscopes. Innovative imaging technologies are now emerging for constructing fine structural features, precise localization and the dynamic interplay of single and macromolecular assemblies that drive cell growth, differentiation and cell death as well as stromal and chromatin remodeling within many cellular context. The newer super-resolution microscopies capture images with unprecedented sensitivity and clarity allowing the exploration of interactions between individual molecules with a distance resolution as low as 20 nm. But these techniques are not robust enough to quantitate molecules on a genome-wide scale. Mass spectrometry imaging is a high-throughput chemical imaging technique for the identification, quantitation and distribution of proteins, lipids and chemical metabolites at picomol level within a single-cell and complex multicellular tissue. Here we provide an overview on imaging instrumentations and computational platforms to store, data mining, analyze and retrieving genomic, proteomic and immunohistochemistry digital image information which are available for multilevel academic-private collaborations. The expansion of these data sets will lead to a merge picture from it we will retrieve knowledge for more rational-design systems to basic and clinical research in near future.
authors.affiliation: Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP, Brazil.
authors.forename: José E
authors.initials: JE
authors.lastname: Belizário
authors.affiliation: Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP, Brazil.
authors.forename: Beatriz A
authors.initials: BA
authors.lastname: Sangiuliano
authors.affiliation: Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP, Brazil.
authors.forename: Marcela
authors.initials: M
authors.lastname: Perez-Sosa
authors.affiliation: Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP, Brazil.
authors.forename: Beatriz V
authors.initials: BV
authors.lastname: Santos
authors.affiliation: Department of Cell Biology and Development, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP, Brazil.
authors.forename: Gláucia
authors.initials: G
authors.lastname: Machado-Santelli
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D040901:Proteomics / Q000379:methods*