title: Development of Biodegradable Poly(citrate)-Polyhedral Oligomeric Silsesquioxanes Hybrid Elastomers with High Mechanical Properties and Osteogenic Differentiation Activity.
abstract: Biodegradable elastomeric biomaterials have attracted much attention in tissue engineering due to their biomimetic viscoelastic behavior and biocompatibility. However, the low mechanical stability at hydrated state, fast biodegradation in vivo, and poor osteogenic activity greatly limited bioelastomers applications in bone tissue regeneration. Herein, we develop a series of poly(octanediol citrate)-polyhedral oligomeric silsesquioxanes (POC-POSS) hybrids with highly tunable elastomeric behavior (hydrated state) and biodegradation and osteoblasts biocompatibility through a facile one-pot thermal polymerization strategy. POC-POSS hybrids show significantly improved stiffness and ductility in either dry or hydrated conditions, as well as good antibiodegradation ability (20-50% weight loss in 3 months). POC-POSS hybrids exhibit significantly enhanced osteogenic differentiation through upregulating alkaline phosphatase (ALP) activity, calcium deposition, and expression of osteogenic markers (ALPL, BGLAP, and Runx2). The high mechanical stability at hydrated state and enhanced osteogenic activity make POC-POSS hybrid elastomers promising as scaffolds and nanoscale vehicles for bone tissue regeneration and drug delivery. This study may also provide a new strategy (controlling the stiffness under hydrated condition) to design advanced hybrid biomaterials with high mechanical properties under physiological condition for tissue regeneration applications.
authors.affiliation: Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an, Shaanxi 710054, China.
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an, Shaanxi 710054, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction , Guangzhou 510000, Guangdong, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Biologic and Materials Sciences, University of Michigan , Ann Arbor 48109-2009, Michigan, United States.|Department of Biomedical Engineering, University of Michigan , Ann Arbor 48109-2009, Michigan, United States.|Macromolecular Science and Engineering Center, University of Michigan , Ann Arbor, Michigan 48109-2009, United States.
authors.forename: Peter X
authors.initials: PX
authors.lastname: Ma
authors.affiliation: Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an, Shaanxi 710054, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lei
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D063566:Biodegradable Plastics / Q000737:chemistry* / Q000627:therapeutic use; D001861:Bone Regeneration; D001842:Bone and Bones / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D019343:Citric Acid / Q000737:chemistry; D020724:Elastomers / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D055595:Mechanical Phenomena; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D063566:Biodegradable Plastics; D020724:Elastomers; D019343:Citric Acid
keywords: biodegradation; hybrid elastomers; mechanical properties; osteogenic differentiation; silica-based biomaterials