title: Physiological Characteristics and Anti-obesity Effect of Lactobacillus plantarum Q180 Isolated from Feces.
abstract: Obesity is strongly associated with several metabolic and chronic diseases and has become a major public health problem of worldwide concern. This study aimed to investigate the physiological characteristics and anti-obesity effects of Lactobacillus plantarum Q180. Lactobacillus plantarum Q180 was isolated from the faces of healthy adults and found to have a lipase inhibitory activity of 83.61±2.32% and inhibited adipocyte differentiation of 3T3-L1 cells (14.63±1.37%) at a concentration of 100 μg/mL. The strain was investigated for its physiological characteristics. The optimum growth temperature of L. plantarum Q180 was 37℃. Lactobacillus plantarum Q180 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed the highest resistance to rifampicin, polymyxin B and vancomycin. The strain showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. It also did not produce carcinogenic enzymes such as β-glucuronidase. The survival rate of L. plantarum Q180 in MRS broth containing 0.3% bile was 97.8%. Moreover, the strain showed a 97.2% survival rate after incubation for 3 h in pH 2.0. Lactobacillus plantarum Q180 was displayed resistance to Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus with rates of 55.6%, 38.0% and 47.6%, respectively. These results demonstrate that L. plantarum Q180 has potential as a probiotic with anti-obesity effects.
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Korea; Department of Food Bioscience and Technology, Korea University, Seoul 136-701, Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Korea.
authors.forename: Seong-A
authors.initials: SA
authors.lastname: Cho
authors.affiliation: Department of Food Bioscience and Technology, Korea University, Seoul 136-701, Korea.
authors.forename: Sae-Hun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Korea.
authors.forename: Sang-Dong
authors.initials: SD
authors.lastname: Lim
keywords: Lactobacillus plantarum; anti-adipogenic activity; anti-lipase activity; physiological characteristics