title: MALDI ionization mechanisms investigated by comparison of isomers of dihydroxybenzoic acid.
abstract: Matrix-assisted laser desorption/ionization (MALDI) ion formation mechanisms were investigated by comparison of isomers of dihydroxybenzoic acid (DHB). These exhibit substantially different MALDI performance, the basis for which was not previously understood. Luminescence decay curves are used here to estimate excited electronic state properties relevant for the coupled chemical and physical dynamics (CPCD) model. With these estimates, the CPCD predictions for relative total ion and analyte ion yields are in good agreement with the data for the DHB isomers. Predictions of a thermal equilibrium model were also compared and found to be incompatible with the data. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Chemistry and Biochemistry, Southern Illinois University Carbondale, Carbondale, IL, 62901, USA.
authors.forename: Kristopher M
authors.initials: KM
authors.lastname: Kirmess
authors.affiliation: RKResearch, Seftigen, Switzerland.
authors.forename: Richard
authors.initials: R
authors.lastname: Knochenmuss
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Blanchard
authors.affiliation: Department of Chemistry and Biochemistry, Southern Illinois University Carbondale, Carbondale, IL, 62901, USA.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Kinsel
mesh_terms: D005841:Gentisates / Q000737:chemistry*; D007536:Isomerism; D049449:Luminescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013816:Thermodynamics
chemical_list: D005841:Gentisates
keywords: CPCD; MALDI; dihydroxybenzoic acid isomers; ionization mechanisms; pooling