title: Magnetic molecularly imprinted polymer nanoparticles for selective solid phase extraction and pre-concentration of Tizanidine in human urine.
abstract: In this work, the magnetic molecularly imprinted polymer nanoparticles (MMIP-NPs) for the selective pre-concentration of Tizanidine have been described. The polymer nanoparticles were synthesized by the polymerization of methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, 2,2-azobisisobutyronitrile as an initiator and Tizanidine as a template molecule. The MMIP-NPs were characterized by scanning electron microscopy (SEM) and thermogravimeteric analysis (TGA). Imprinted Tizanidine molecules were removed from the polymeric structure using acetic acid in methanol (10:90 V/V%), as the eluent solvent. The limits of detection (L.O.D) for Tizanidine were 1.13×10(-6)M and 1.68×10(-6)M in ultrapure water and urine, respectively. Also, the relative standard deviations (R.S.D) in ultrapure water and urine were 2.21% and 2.58%, respectively. The method was applied to the determination of Tizanidine in the human urine samples.
authors.affiliation: Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Golaleh
authors.initials: G
authors.lastname: Sheykhaghaei
authors.affiliation: Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Moayad
authors.initials: M
authors.lastname: Hossainisadr
authors.affiliation: Department of Chemistry, Mahabad Branch, Islamic Azad University, Mahabad, P.O. Box 443, Iran. Electronic address: khanahmadzadeh@iau-mahabad.ac.Ir.
authors.forename: Salah
authors.initials: S
authors.lastname: Khanahmadzadeh
authors.affiliation: Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Mirabdollah
authors.initials: M
authors.lastname: Seyedsajadi
authors.affiliation: Department of Chemistry, Mahabad Branch, Islamic Azad University, Mahabad, P.O. Box 443, Iran.
authors.forename: Awat
authors.initials: A
authors.lastname: Alipouramjad
mesh_terms: D003000:Clonidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013696:Temperature
chemical_list: D011108:Polymers; C023754:tizanidine; D003000:Clonidine
keywords: Human urine; Magnetic nanoparticles; Magnetic solid phase extraction (MSPE); Molecularly imprinted polymer; Ttizanidine