title: Expression and significance of CD4(+)CD25(+)CD127(-) regulatory T cells in peripheral blood of patients with different phenotypes of Guillain-Barré syndrome.
abstract: OBJECTIVE
  This study aims to investigate the changes of immune status and significance in patients with Guillain-Barré syndrome (GBS).
  
  
  METHODS
  The proportion of CD4(+)CD25(+)CD127(-) regulatory T cells in peripheral blood before immunotherapy for 41 patients with GBS (including 29 classic type and 12 variant type) and 42 normal control patients (healthy volunteers) were evaluated by flow cytometry. And molybdenum three phenol red method was used to detect cerebrospinal fluid protein content of 28 patients with GBS (including 19 with classic type and 9 with variant type).
  
  
  RESULTS
  Compared with healthy control group, the CD4(+)CD25(+)CD127(-) of GBS group had obvious difference (P<0.05). Of which, the CD4(+)CD25(+)CD127(-) regulatory T cells of the classic GBS group had no significant changes compared with the variant group (P>0.05), as well as the cerebrospinal fluid protein content between classic and variant GBS groups. The decrease of the proportion of CD4(+)CD25(+)CD127(-) regulatory T cells suggested abnormal expression of immune function in GBS patients.
  
  
  CONCLUSIONS
  The decrease of GBS regulatory T cell number or function indicated that the immune regulatory T cells mediated imbalance of immune regulation involved in the pathogenesis of GBS.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
authors.forename: Shu-Chao
authors.initials: SC
authors.lastname: Pan
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
authors.forename: An-Qiang
authors.initials: AQ
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Qian
keywords: Guillain-Barré syndrome; Regulatory T cells; flow cytometry