title: Differential immune response of vitellogenin gene to Vibrio anguillarum in noble scallop Chlamys nobilis and its correlation with total carotenoid content.
abstract: Vitellogenin (Vg), an egg yolk precursor protein, not only functions as a source of nutrients and a nonpolar molecular carrier that combine and transfer lipids, proteins, vitamin and carotenoids to oocytes during the oogenesis. but also links with the immune defense in many oviparous animals. To investigate whether Vg plays a immune defensive role in noble scallop Chlamys nobilis, an acute Vibrio anguillarum infection experiment was conducted in orange and brown scallops with different carotenoids content. qRT-PCR result showed that Vg transcripts were significantly up-regulated after challenge with V. anguillarum in orange and brown shell scallops compared to the control group and Vg expression reached the highest spot at 6 h, indicated that Vg possessed an immune function in the noble scallop. Interestingly, a significantly positive correlation between Vg transcript levels and total carotenoids content in the ovary was observed, indicating that Vg gene expression was up regulated by carotenoids. The results suggest that Vg is a potent immune protector and carotenoid may linked with Vg plays an important role in host immune system against pathogens in noble scallop C. nobilis.
authors.affiliation: Key Laboratory of Marine Biotechnology of Guangdong Province, Shantou University, Shantou 515063, China; Mariculture Research Center for Subtropical Shellfish & Algae of Guangdong Province, Shantou 515063, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Biotechnology of Guangdong Province, Shantou University, Shantou 515063, China; Mariculture Research Center for Subtropical Shellfish & Algae of Guangdong Province, Shantou 515063, China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory of Marine Biotechnology of Guangdong Province, Shantou University, Shantou 515063, China; Mariculture Research Center for Subtropical Shellfish & Algae of Guangdong Province, Shantou 515063, China. Electronic address: hpzheng@stu.edu.cn.
authors.forename: Huaiping
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Marine Biotechnology of Guangdong Province, Shantou University, Shantou 515063, China; Mariculture Research Center for Subtropical Shellfish & Algae of Guangdong Province, Shantou 515063, China.
authors.forename: Helu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Biotechnology of Guangdong Province, Shantou University, Shantou 515063, China; Mariculture Research Center for Subtropical Shellfish & Algae of Guangdong Province, Shantou 515063, China.
authors.forename: Shengkang
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D049897:Pectinidae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D015854:Up-Regulation; D014733:Vibrio / Q000502:physiology*; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D014819:Vitellogenins
keywords: Carotenoid; Chlamys nobilis; Vibrio anguillarum; Vitellogenin gene