title: Comprehensive Identification and Bread-Making Quality Evaluation of Common Wheat Somatic Variation Line AS208 on Glutenin Composition.
abstract: High molecular weight glutenin subunits (HMW-GSs) are important seed storage proteins in wheat (Triticum aestivum) that determine wheat dough elasticity and processing quality. Clarification of the defined effectiveness of HMW-GSs is very important to breeding efforts aimed at improving wheat quality. To date, there have no report on the expression silencing and quality effects of 1Bx20 and 1By20 at the Glu-B1 locus in wheat. A wheat somatic variation line, AS208, in which both 1Bx20 and 1By20 at Glu-B1 locus were silenced, was developed recently in our laboratory. Evaluation of agronomic traits and seed storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high performance liquid chromatography (RP-HPLC) indicated that AS208 was highly similar to its parental cultivar Lunxuan987 (LX987), with the exception that the composition and expression of HMW-GSs was altered. The 1Bx20 and 1By20 in AS208 were further identified to be missing by polymerase chain reaction (PCR) and quantitative real-time RT-PCR (qRT-PCR) assays. Based on the PCR results for HMW-GS genes and their promoters in AS208 compared with LX987, 1Bx20 and 1By20 were speculated to be deleted in AS208 during in vitro culture. Quality analysis of this line with Mixograph, Farinograph, and Extensograph instruments, as well as analysis of bread-making quality traits, demonstrated that the lack of the genes encoding 1Bx20 and 1By20 caused various negative effects on dough processing and bread-making quality traits, including falling number, dough stability time, mixing tolerance index, crude protein values, wet gluten content, bread size, and internal cell structure. AS208 can potentially be used in the functional dissection of other HMW-GSs as a plant material with desirable genetic background, and in biscuit making industry as a high-quality weak gluten wheat source.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100048, China.
authors.forename: Lele
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Shunli
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Lipu
authors.initials: L
authors.lastname: Du
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, 250100, China.
authors.forename: Xinyou
authors.initials: X
authors.lastname: Cao
authors.affiliation: Institute of Agricultural Sciences of Lixiahe Districts, Yangzhou, Jiangsu, 225007, China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100048, China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Ye
mesh_terms: D001939:Bread / Q000032:analysis* / Q000592:standards*; D063427:Food Quality; D020869:Gene Expression Profiling; D014644:Genetic Variation; D005983:Glutens / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D005983:Glutens; C037302:glutenin