title: [Effect of zoledronic acid on cell proliferation and apoptosis of human periodontal fibroblasts].
abstract: OBJECTIVE
  To observe the effect of different concentrations of zoledronic acid on human gingival fibroblast (HGF) cells proliferation and apoptosis, and to investigate the mechanism of bisphosphonate-related osteonecrosis of the jaw (BRONJ) caused by zoledronic acid.
  
  
  METHODS
  Different concentrations (0, 0.5, l.0, 5.0, 10.0 µmol/L) of zoledronic acid acted on the HGF. After 24 hours, flow cytometry was used to detect the rate of apoptosis and methyl thiazolyl terazolium (MTT) assay used to observe the proliferation of HGF. The effect of different concentrations of zoledronic acid on cell proliferation was examined by proliferation test on day 2, 4 and 7.
  
  
  RESULTS
  Flow cytometry showed that when the concentration of zoledronic acid was 0, 0.5, 1.0, 5.0, 10.0 µmol/L, the apoptosis rate was (0.67 ± 0.50)%, (2.13 ± 0.21)%, (3.27 ± 0.23)%, (4.17 ± 0.35)%, (9.87 ± 1.79)% respectively. The difference in HGF cells between control group and zoledronic acid groups was significant (P < 0.05) at three concentrations of zoledronic acid (1.0, 5.0, 10.0 µmol/L). The cell proliferation test showed an concentration-time dependent effect. With increase of concentration and time, the cell proliferation was significantly inhibited. There was significant difference in absorbance value among the different concentration groups (P < 0.05).
  
  
  CONCLUSIONS
  Zoledronic acid can inhibit HGF proliferation and promote its apoptosis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China.
authors.forename: Qingbiao
authors.initials: Q
authors.lastname: Fu
authors.forename: Caiwen
authors.initials: C
authors.lastname: Cui
authors.forename: Bin
authors.initials: B
authors.lastname: Xuan
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Guo
authors.forename: Chunlin
authors.initials: C
authors.lastname: Liu
authors.affiliation: Email: zj301doctor@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D059266:Bisphosphonate-Associated Osteonecrosis of the Jaw / Q000209:etiology; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D004396:Coloring Agents; D004164:Diphosphonates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D004396:Coloring Agents; D004164:Diphosphonates; D007093:Imidazoles; D013778:Tetrazolium Salts; D013844:Thiazoles; D000077211:Zoledronic Acid; C022616:thiazolyl blue