title: PGC-1α overexpression via local transfection attenuates mitophagy pathway in muscle disuse atrophy.
abstract: Loss of mitochondrial structural and functional integrity plays a critical role in the pathogenesis of muscle disuse atrophy. Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) has been suggested to modulate autophagy-lysosome pathway (mitophagy) during muscle atrophy, but clear evidence is still lacking. In the current study, we tested the hypothesis that overexpression of PGC-1α via in vivo transfection would ameliorate mitophagy in mouse tibialis anterior muscle subjected to two weeks of immobilization (IM), followed by remobilization (RM). While mitochondrial biogenesis and antioxidant enzymes are decreased, all autophagic and mitophagic protein markers such as Beclin-1, Bnip3, PINK1, parkin, Mul1 and the LC3II/LC3I ratio were increased in IM-RM muscle together with activation of FoxO pathway. Overexpression of PGC-1α significantly increased mitochondrial DNA proliferation and oxidative enzyme activity, whereas it mitigated oxidative stress and mitochondrial ubiquination in IM-RM muscle. Protein contents of PINK1, parkin and Mul1 in mitochondria decreased by approximately 50% with PGC-1α treatment. Furthermore, PGC-1α overexpression suppressed FoxO1 and FoxO3 activation along with a decreased LC3II/LC3I ratio. Importantly, PGC-1α attenuated IM-RM-induced ubiquination and degradation of mitofusion protein Mfn2. Muscle apoptotic tendency, measured by Bax/Bcl2 ratio and caspase-3 activity, were elevated with IM-RM, but unaffected by PGC-1α. We conclude that overexpression of PGC-1α by in vivo transfection can inhibit activation of mitophagy pathway in the atrophying muscle caused by immobilization.
authors.affiliation: Laboratory of Physiological Hygiene and Exercise Science, School of Kinesiology, University of Minnesota Twin Cities, Minneapolis, 1900 University Avenue, Minneapolis, MN 55455, USA.
authors.forename: Chounghun
authors.initials: C
authors.lastname: Kang
authors.affiliation: Laboratory of Physiological Hygiene and Exercise Science, School of Kinesiology, University of Minnesota Twin Cities, Minneapolis, 1900 University Avenue, Minneapolis, MN 55455, USA. Electronic address: llji@umn.edu.
authors.forename: Li Li
authors.initials: LL
authors.lastname: Ji
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D063306:Mitophagy / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009135:Muscular Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020966:Muscular Disorders, Atrophic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000096:biosynthesis* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D014162:Transfection
chemical_list: D004272:DNA, Mitochondrial; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D017382:Reactive Oxygen Species
keywords: Apoptosis; Immobilization; Mitochondria; Mitophagy; Muscle; Muscle disuse atrophy; Oxidative stress; PGC-1α overexpression