title: Expression profile of interferon tau-stimulated genes in ovine peripheral blood leukocytes during embryonic death.
abstract: Early and efficient detection of embryonic death (ED) has a valuable impact as important as early pregnancy diagnosis in ruminants. Among early pregnancy diagnosis methods, detection of the expression of interferon tau-stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) is well documented in cows and ewes. Therefore, we hypothesized that the expression profile of ISGs in PBLs might also be useful for detecting ED in these animals. For this purpose, pregnant ewes were used as an experimental model. Pregnancy was detected on Day 18 after mating by transrectal ultrasonography. Pregnant ewes were divided into a control group (sham injection on Day 18, n = 10) and ED group (treated with 75 μg synthetic PGF2α on Day 18, n = 12). PBLs and plasma were collected on Days 0 (mating day), 15, 18, 19, 20, 21, 23, and 25 by jugular venipuncture. Total RNA was isolated from PBLs. ISGs expression levels were determined by real-time polymerase chain reaction in triplicate. Electrochemiluminescence immunoassay was used to measure progesterone (P4) levels in plasma. In the ED group, the P4 level declined to less than 1 ng/mL on Day 19 and remained at a low level until the end of the study. Compared with that on Day 0, receptor transporter protein 4 (RTP4) and ISG15 expression was upregulated on Day 15 and remained high until Day 21 in both groups, and RTP4 and ISG15 mRNA levels were attenuated on Days 23 and 25 only in the ED group (P < 0.001). Myxovirus resistance 1 expression was upregulated on Day 15 and remained high until Day 23 in both groups, but was attenuated on Day 25 in the ED group (P < 0.05). The B2-microglobulin mRNA level did not change significantly during the study in either group. These results indicate that the decline in P4 concentration was an immediate response to PGF2α and that the embryo may have survived longer than the CL on the basis of the extended period of ISGs expression. This suggests that the absence of P4 could be the reason for ED rather than a direct effect of PGF2α. In conclusion, the expression of ISGs, including ISG15, RTP4, and myxovirus resistance 1, but not B2-microglobulin, in PBLs may serve as a marker of ED.
authors.affiliation: Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Dicle University, Diyarbakir, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Kose
authors.affiliation: Faculty of Veterinary Medicine, Department of Physiology, Dicle University, Diyarbakir, Turkey.
authors.forename: M S
authors.initials: MS
authors.lastname: Kaya
authors.affiliation: Faculty of Veterinary Medicine, Department of Physiology, Dicle University, Diyarbakir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Aydilek
authors.affiliation: Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Dicle University, Diyarbakir, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Kucukaslan
authors.affiliation: Faculty of Veterinary Medicine, Department of Animal Husbandry, Dicle University, Diyarbakir, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Bayril
authors.affiliation: Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Dicle University, Diyarbakir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Bademkiran
authors.affiliation: Faculty of Agriculture, Department of Animal Husbandry, Osmangazi University, Eskisehir, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Kiyma
authors.affiliation: Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Dicle University, Diyarbakir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Ozyurtlu
authors.affiliation: Faculty of Medicine, Department of Biostatistics, Karabuk University, Karabuk, Turkey.
authors.forename: S A
authors.initials: SA
authors.lastname: Kayis
authors.affiliation: Faculty of Veterinary Medicine, Department of Genetics, Selcuk University, Konya, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Guzeloglu
authors.affiliation: Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Dicle University, Diyarbakir, Turkey. Electronic address: moatli@hotmail.com.
authors.forename: M O
authors.initials: MO
authors.lastname: Atli
mesh_terms: D000818:Animals; D020964:Embryo Loss; D005260:Female; D005786:Gene Expression Regulation; D007118:Immunoassay; D007370:Interferon Type I / Q000494:pharmacology*; D007962:Leukocytes / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D012756:Sheep; D059467:Transcriptome
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; D012333:RNA, Messenger; C020947:interferon tau; D011374:Progesterone
keywords: Embryonic death; Ewe; Interferon tau–stimulated genes; Peripheral blood leukocytes