title: Effects of BmCPV Infection on Silkworm Bombyx mori Intestinal Bacteria.
abstract: The gut microbiota has a crucial role in the growth, development and environmental adaptation in the host insect. The objective of our work was to investigate the microbiota of the healthy silkworm Bombyx mori gut and changes after the infection of B. mori cypovirus (BmCPV). Intestinal contents of the infected and healthy larvae of B. mori of fifth instar were collected at 24, 72 and 144 h post infection with BmCPV. The gut bacteria were analyzed by pyrosequencing of the 16S rRNA gene. 147(135) and 113(103) genera were found in the gut content of the healthy control female (male) larvae and BmCPV-infected female (male) larvae, respectively. In general, the microbial communities in the gut content of healthy larvae were dominated by Enterococcus, Delftia, Pelomonas, Ralstonia and Staphylococcus, however the abundance change of each genus was depended on the developmental stage and gender. Microbial diversity reached minimum at 144 h of fifth instar larvae. The abundance of Enterococcus in the females was substantially lower and the abundance of Delftia, Aurantimonas and Staphylococcus was substantially higher compared to the males. Bacterial diversity in the intestinal contents decreased after post infection with BmCPV, whereas the abundance of both Enterococcus and Staphylococcus which belongs to Gram-positive were increased. Therefore, our findings suggested that observed changes in relative abundance was related to the immune response of silkworm to BmCPV infection. Relevance analysis of plenty of the predominant genera showed the abundance of the Enterococcus genus was in negative correlation with the abundance of the most predominant genera. These results provided insight into the relationship between the gut microbiota and development of the BmCPV-infected silkworm.
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Kumar
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Gong
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Liang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Sulan
authors.initials: S
authors.lastname: Kuang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 215123, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 215123, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 215123, China.
authors.forename: Renyu
authors.initials: R
authors.lastname: Xue
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 215123, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Gong
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D012831:Bombyx / Q000276:immunology / Q000382:microbiology*; D005260:Female; D000069196:Gastrointestinal Microbiome; D054884:Host-Pathogen Interactions; D007814:Larva / Q000276:immunology / Q000382:microbiology; D008297:Male; D012087:Reoviridae / Q000276:immunology*