title: Profiling the decomposition odour at the grave surface before and after probing.
abstract: Human remains detection (HRD) dogs are recognised as a valuable and non-invasive search method for remains concealed in many different environments, including clandestine graves. However, the search for buried remains can be a challenging task as minimal odour may be available at the grave surface for detection by the dogs. Handlers often use a soil probe during these searches in an attempt to increase the amount of odour available for detection, but soil probing is considered an invasive search technique. The aim of this study was to determine whether the soil probe assists with increasing the abundance of volatile organic compounds (VOCs) available at the grave surface. A proof-of-concept method was developed using porcine remains to collect VOCs within the grave without disturbing the burial environment, and to compare their abundance at the grave surface before and after probing. Detection and identification of the VOC profiles required the use of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) due to its superior sensitivity and selectivity for decomposition odour profiling. The abundance of decomposition VOCs was consistently higher within the grave environment compared to the grave surface, except when the grave surface had been disturbed, confirming the reduced availability of odour at the grave surface. Although probing appeared to increase the abundance of VOCs at the grave surface on many of the sampling days, there were no clear trends identified across the study and no direct relationships with the environmental variables measured. Typically, the decomposition VOCs that were most prevalent in the grave soil were the same VOCs detected at the grave surface, whereas the trace VOCs detected in these environments varied throughout the post-burial period. This study highlighted that probing the soil can assist with releasing decomposition VOCs but is likely correlated to environmental and burial variables which require further study. The use of a soil probe to assist HRD dogs should not be disregarded but should only follow the use of non-invasive methods if deemed appropriate.
authors.affiliation: Centre for Forensic Science, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia. Electronic address: shari.forbes@uts.edu.au.
authors.forename: S L
authors.initials: SL
authors.lastname: Forbes
authors.affiliation: Centre for Forensic Science, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.
authors.forename: A N
authors.initials: AN
authors.lastname: Troobnikoff
authors.affiliation: Centre for Forensic Science, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Ueland
authors.affiliation: Centre for Forensic Science, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.
authors.forename: K D
authors.initials: KD
authors.lastname: Nizio
authors.affiliation: Centre for Forensic Science, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.
authors.forename: K A
authors.initials: KA
authors.lastname: Perrault
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D009812:Odorants / Q000032:analysis*; D011180:Postmortem Changes; D012987:Soil; D013552:Swine; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D012987:Soil; D055549:Volatile Organic Compounds
keywords: Buried remains; Comprehensive two-dimensional gas chromatography–mass spectrometry; Forensic taphonomy; GC×GC–TOFMS; Soil probing; Volatile organic compounds