title: Honey Bee Allatostatins Target Galanin/Somatostatin-Like Receptors and Modulate Learning: A Conserved Function?
abstract: Sequencing of the honeybee genome revealed many neuropeptides and putative neuropeptide receptors, yet functional characterization of these peptidic systems is scarce. In this study, we focus on allatostatins, which were first identified as inhibitors of juvenile hormone synthesis, but whose role in the adult honey bee (Apis mellifera) brain remains to be determined. We characterize the bee allatostatin system, represented by two families: allatostatin A (Apime-ASTA) and its receptor (Apime-ASTA-R); and C-type allatostatins (Apime-ASTC and Apime-ASTCC) and their common receptor (Apime-ASTC-R). Apime-ASTA-R and Apime-ASTC-R are the receptors in bees most closely related to vertebrate galanin and somatostatin receptors, respectively. We examine the functional properties of the two honeybee receptors and show that they are transcriptionally expressed in the adult brain, including in brain centers known to be important for learning and memory processes. Thus we investigated the effects of exogenously applied allatostatins on appetitive olfactory learning in the bee. Our results show that allatostatins modulate learning in this insect, and provide important insights into the evolution of somatostatin/allatostatin signaling.
authors.affiliation: Department of Zoology, Dunedin, Otago, New Zealand.|Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur la Cognition Animale (UMR 5169), Toulouse, France.|Université de Toulouse, UPS Centre de Recherches sur la Cognition Animale (UMR 5169), Toulouse, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Urlacher
authors.affiliation: CNRS, UMR 5203, Institut de Génomique Fonctionnelle, Montpellier, France.|INSERM, U1191, Montpellier, France.|Université de Montpellier, UMR 5203, Montpellier, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Soustelle
authors.affiliation: CNRS, UMR 5203, Institut de Génomique Fonctionnelle, Montpellier, France.|INSERM, U1191, Montpellier, France.|Université de Montpellier, UMR 5203, Montpellier, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Parmentier
authors.affiliation: Department of Animal Physiology and Neurobiology, Zoological Institute, KU Leuven, Leuven, Belgium.
authors.forename: Heleen
authors.initials: H
authors.lastname: Verlinden
authors.affiliation: EA 4552 Réceptorologie et ciblage thérapeutique en cancérologie, Université de Toulouse, UPS, Toulouse, France.
authors.forename: Marie-Julie
authors.initials: MJ
authors.lastname: Gherardi
authors.affiliation: EA 4552 Réceptorologie et ciblage thérapeutique en cancérologie, Université de Toulouse, UPS, Toulouse, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fourmy
authors.affiliation: Department of Zoology, Dunedin, Otago, New Zealand.
authors.forename: Alison R
authors.initials: AR
authors.lastname: Mercer
authors.affiliation: Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur la Cognition Animale (UMR 5169), Toulouse, France.|Université de Toulouse, UPS Centre de Recherches sur la Cognition Animale (UMR 5169), Toulouse, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Devaud
authors.affiliation: Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur la Cognition Animale (UMR 5169), Toulouse, France.|Université de Toulouse, UPS Centre de Recherches sur la Cognition Animale (UMR 5169), Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Massou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001070:Appetitive Behavior / Q000502:physiology; D001516:Bees / Q000145:classification / Q000502:physiology*; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology; D017124:Conserved Sequence; D019004:Galanin / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism; D007858:Learning / Q000502:physiology; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D055696:Olfactory Perception / Q000502:physiology; D010802:Phylogeny; D044088:Receptors, Galanin / Q000235:genetics* / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013004:Somatostatin / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D007605:Juvenile Hormones; D009479:Neuropeptides; D044088:Receptors, Galanin; D017481:Receptors, Somatostatin; C076523:allatostatin; D013004:Somatostatin; D019004:Galanin