title: Electrochemical Fabrication of Functional Gelatin-Based Bioelectronic Interface.
abstract: Gelatin remains one of the most important biopolymeric material platforms because of its availability, safety, biocompatibility, biodegradability, and stimuli-responsive properties. Here we report a simple, rapid, and reagentless anodic deposition method to assemble gelatin hydrogels from aqueous salt solutions onto an electrode surface. Results indicate that anodic reactions partially oxidize gelatin to yield a covalently cross-linked network that can perform multiple functions. First, anodically deposited gelatin remains activated, allowing covalent protein grafting and thus enabling biofunctionalization for electrochemical biosensing. Second, the anodically deposited gelatin retains its thermally responsive physical cross-linking properties that enable switching functions. Finally, the physical and chemical cross-linking mechanisms are reversible, which enables self-healing functions. Thus, anodic deposition provides a facile method to assemble gelatin-based multifunctional matrices for diverse applications in bioelectronics.
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland , College Park, Maryland 20742, United States.|Key Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, Jianghan University , Wuhan 430056, People's Republic of China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland , College Park, Maryland 20742, United States.|Fischell Department of Bioengineering, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland , College Park, Maryland 20742, United States.|Fischell Department of Bioengineering, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Payne
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005780:Gelatin; D005949:Glucose Oxidase