title: Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera.
abstract: Potent Botulinum neurotoxins (BoNTs) represent a threat to public health and safety. Botulism is a disease caused by BoNT intoxication that results in muscle paralysis that can be fatal. Sensitive assays capable of detecting BoNTs from different substrates and settings are essential to limit foodborne contamination and morbidity. In this report, we describe a rapid 96-well microfluidic double sandwich immunoassay for the sensitive detection of BoNT-A from animal sera. This BoNT microfluidic assay requires only 5 μL of serum, provides results in 75 min using a standard fluorescence microplate reader and generates minimal hazardous waste. The assay has a <30 pg·mL(-1) limit of detection (LOD) of BoNT-A from spiked human serum. This sensitive microfluidic BoNT-A assay offers a fast and simplified workflow suitable for the detection of BoNT-A from serum samples of limited volume in most laboratory settings.
authors.affiliation: Produce Safety & Microbiology Research Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA. lmar.babrak@ars.usda.gov.
authors.forename: Lmar
authors.initials: L
authors.lastname: Babrak
authors.affiliation: Produce Safety & Microbiology Research Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA. alice.lin@ars.usda.gov.
authors.forename: Alice
authors.initials: A
authors.lastname: Lin
authors.affiliation: Foodborne Toxin Detection and Prevention Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA. larry.stanker@ars.usda.gov.
authors.forename: Larry H
authors.initials: LH
authors.lastname: Stanker
authors.affiliation: Foodborne Toxin Detection and Prevention Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA. jeffery.mcgarvey@ars.usda.gov.
authors.forename: Jeffery
authors.initials: J
authors.lastname: McGarvey
authors.affiliation: Produce Safety & Microbiology Research Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA. robert.hnasko@ars.usda.gov.
authors.forename: Robert
authors.initials: R
authors.lastname: Hnasko
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology; D019274:Botulinum Toxins, Type A / Q000032:analysis / Q000097:blood* / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae; D057141:Food, Preserved / Q000032:analysis; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D006736:Horses; D006801:Humans; D057230:Limit of Detection; D051379:Mice; D046210:Microfluidic Analytical Techniques; D009498:Neurotoxins / Q000032:analysis / Q000097:blood* / Q000276:immunology; D044967:Serum / Q000737:chemistry; D012756:Sheep
chemical_list: D055910:Antibodies, Immobilized; D009498:Neurotoxins; D019274:Botulinum Toxins, Type A
keywords: animal serum; botulinum neurotoxin (BoNT); botulism; diagnostic; immunoassay; microfluidic; rapid detection; toxin