title: [Correlation of HPLC Characteristic Spectra of Vinegar Corydalis Rhizoma Decoction Pieces, Water Decoction and Formula Granules].
abstract: OBJECTIVE
  To study the correlation of characteristic spectra of Vinegar Corydalis Rhizoma decoction pieces, water decoction and formula granules by HPLC, and to investigate the transfer of the main chemical constituents between three different forms.
  
  
  METHODS
  The analysis was carried out by a Phenomenex Gemini C18 column (250 mm x 4.6 mm,5 μm) with acetonitrile-1% acetic acid and ammonium acetate buffer solution (pH 6.0) as the mobile phase in a gradient elution mode. The detection wavelength was 280 nm with a flow rate of 0.8 mL /min. The column temperature was 30 degrees C. The characteristic spectra from 11 batches of Vinegar Corydalis Rhizoma decoction pieces, 11 batches of water decoction and 11 batches of formula granules were established respectively.
  
  
  RESULTS
  Ten peaks in the HPLC characteristic spectra from 11 batches of formula granules could be tracked in the water decoction, nine peaks in the HPLC characteristic spectra could be tracked in the decoction pieces. In the ten common peaks, four components such as protopine, palnatine chloride, berberine hydrochloride and tetrahydropalmatine were verified.
  
  
  CONCLUSIONS
  The main chemical components of Vinegar Corydalis Rhizoma decoction pieces, water decoction and formula granules are basically the same, the common component contents have similar proportion.
authors.forename: Mei
authors.initials: M
authors.lastname: Wei
authors.forename: Lan-zhe
authors.initials: LZ
authors.lastname: Du
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Guang-da
authors.initials: GD
authors.lastname: Zhang
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Chen
mesh_terms: D019342:Acetic Acid; D053119:Benzophenanthridines; D001599:Berberine; D001600:Berberine Alkaloids; D002851:Chromatography, High Pressure Liquid; D031311:Corydalis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D013696:Temperature; D014867:Water
chemical_list: D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D014867:Water; D001599:Berberine; C014215:tetrahydropalmatine; D019342:Acetic Acid; C009093:protopine