title: Separation and preparation of 6-gingerol from molecular distillation residue of Yunnan ginger rhizomes by high-speed counter-current chromatography and the antioxidant activity of ginger oils in vitro.
abstract: Molecular distillation residue (MD-R) from ginger had the most total phenol content of 247.6mg gallic acid equivalents per gram (GAE/g) among the ginger oils. High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale was successfully performed in separation and purification of 6-gingerol from MD-R by using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10:2:5:7, v/v/v/v). The target compound was isolated, collected, purified by HSCCC in the head-tail mode, and then analyzed by HPLC. A total of 90.38±0.53mg 6-gingerol was obtained from 600mg MD-R, with purity of 99.6%. In addition, the structural identification of 6-gingerol was performed by EI/MS, (1)H NMR and (13)C NMR. Moreover, the orders of antioxidant activity were vitamin E (VE)>supercritical fluid extraction oleoresin (SFE-O)=MD-R=6-gingerol>molecular distillation essential oil (MD-EO) and butylated hydroxytoluene (BHT)=VE>6-gingerol>MD-R=SFE-O>MD-EO, respectively in 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and β-Carotene bleaching.
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Gan
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liang
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Chen
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wen
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: niyy102@163.com.
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Ni
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000032:analysis / Q000378:metabolism; D002396:Catechols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D057168:Distillation; D005233:Fatty Alcohols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D020939:Zingiber officinale / Q000737:chemistry*; D010851:Picrates / Q000032:analysis / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002396:Catechols; D005233:Fatty Alcohols; D010851:Picrates; D010938:Plant Oils; C007845:gingerol; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: 6-Gingerol; Antioxidant activity; High-speed counter-current chromatography (HSCCC); Molecular distillation residue