title: Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin.
abstract: The present study aimed at biochemical and molecular characterization of Escherichia coli strains isolated from horticultural products and irrigation water of Cotonou. The samples were collected from 12 market gardeners of 4 different sites. Rapid' E. coli medium was used for identification of E. coli strains and the antimicrobial susceptibility was performed by the agar disk diffusion method. The β-lactamases production was sought by the liquid acidimetric method. The genes coding for β-lactamases and toxins were identified by PCR method. The results revealed that about 34.95% of the analyzed samples were contaminated by E. coli. Cabbages were the most contaminated by E. coli (28.26%) in dry season. All isolated strains were resistant to amoxicillin. The penicillinase producing E. coli carried blaTEM (67.50%), blaSHV (10%), and blaCTX-M (22.50%) genes. The study revealed that the resistance genes such as SLTI (35.71%), SLTII (35.71%), ETEC (7.15%), and VTEC (21.43%) were carried. Openly to the found results and considering the importance of horticultural products in Beninese food habits, it is important to put several strategies aiming at a sanitary security by surveillance and sensitization of all the actors on the risks of some practices.
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Wassiyath
authors.initials: W
authors.lastname: Moussé
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Haziz
authors.initials: H
authors.lastname: Sina
authors.affiliation: Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d'Abomey-Calavi, ISBA-Champ de foire, 01 BP 526 Cotonou, Benin.
authors.forename: Farid
authors.initials: F
authors.lastname: Baba-Moussa
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Pacôme A
authors.initials: PA
authors.lastname: Noumavo
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Nadège A
authors.initials: NA
authors.lastname: Agbodjato
authors.affiliation: Centre de Recherches Agricoles Sud, Institut National des Recherches Agricoles du Bénin, Attogon, BP 884 Cotonou, Benin.
authors.forename: Adolphe
authors.initials: A
authors.lastname: Adjanohoun
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Lamine
authors.initials: L
authors.lastname: Baba-Moussa
mesh_terms: D000383:Agriculture; D001541:Benin; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000235:genetics / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D014675:Vegetables / Q000382:microbiology; D014871:Water Microbiology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001618:beta-Lactamases