title: Association of cord blood des-acyl ghrelin with birth weight, and placental GHS-R1 receptor expression in SGA, AGA, and LGA newborns.
abstract: Although ghrelin in cord blood has been associated to birth weight, its role in fetal and postnatal growth has not been elucidated. The aim of this study was to analyze total ghrelin, acyl ghrelin (AG), and des-acyl ghrelin (DAG) in cord blood of newborns with idiopathic birth weight alterations, and to evaluate protein expression of placental GHS-R1, in order to investigate their correlation with birth weight and placental weight. We performed a cross-sectional comparative study in umbilical cord blood and placentas from healthy mothers of SGA, AGA, and LGA (small, adequate and large for gestational age) term newborns (n = 20 per group). Cord blood total ghrelin, AG, and DAG were measured by ELISA, and placental GHS-R1 expression was evaluated by Western blot. Cord blood DAG was higher in SGA compared to AGA newborns (902.1 ± 109.1 and 597.4 ± 58.2 pg/ml, respectively, p = 0.01) while LGA and AGA showed similar values (627.2 ± 76.4 pg/ml for LGA, p = 0.80). DAG negatively correlated with birthweight (r = -0.31, p = 0.02) and placental weight (r = -0.33, p = 0.02). No differences in AG or total ghrelin were found. GHS-R1 protein in placenta was not differentially expressed among SGA, AGA, and LGA. Our results suggest a role of DAG in intrauterine growth. Further studies are needed in order to elucidate the mechanisms by which DAG participates in fetal growth.
authors.affiliation: Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus. 20 de Enero No. 929 Col. Obregon, 37320, Leon, Guanajuato, Mexico.
authors.forename: Martha I
authors.initials: MI
authors.lastname: González-Domínguez
authors.affiliation: Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus. 20 de Enero No. 929 Col. Obregon, 37320, Leon, Guanajuato, Mexico.
authors.forename: Maria-Luisa
authors.initials: ML
authors.lastname: Lazo-de-la-Vega-Monroy
authors.affiliation: Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus. 20 de Enero No. 929 Col. Obregon, 37320, Leon, Guanajuato, Mexico.
authors.forename: Silvio
authors.initials: S
authors.lastname: Zaina
authors.affiliation: Biology Department, Natural and Exact Sciences Division, University of Guanajuato, Guanajuato Campus, Guanajuato, Mexico.
authors.forename: Myrna
authors.initials: M
authors.lastname: Sabanero
authors.affiliation: UMAE No. 48 IMSS, Leon, Guanajuato, Mexico.
authors.forename: Leonel
authors.initials: L
authors.lastname: Daza-Benítez
authors.affiliation: Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus. 20 de Enero No. 929 Col. Obregon, 37320, Leon, Guanajuato, Mexico.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Malacara
authors.affiliation: Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus. 20 de Enero No. 929 Col. Obregon, 37320, Leon, Guanajuato, Mexico. gloriabs70@hotmail.com.
authors.forename: Gloria
authors.initials: G
authors.lastname: Barbosa-Sabanero
mesh_terms: D000293:Adolescent; D000328:Adult; D001724:Birth Weight / Q000502:physiology*; D003430:Cross-Sectional Studies; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005865:Gestational Age; D054439:Ghrelin / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D007236:Infant, Small for Gestational Age; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D054440:Receptors, Ghrelin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D054439:Ghrelin; D054440:Receptors, Ghrelin; C422650:ghrelin, des-n-octanoyl
keywords: Birth weight; Des-acyl ghrelin; GHS-R1; Placenta