title: Dynamic Micropatterning of Cells on Nanostructured Surfaces Using a Cell-friendly Photoresist.
abstract: Cellular dynamics under complex topographical microenvironments are important for many biological processes in development and diseases, but systematic investigation has been limited due to the lack of technology. Herein, we developed a new dynamic cell patterning method based on a cell-friendly photoresist polymer that allows in situ control of cell dynamics on nanostructured surfaces. Using this method, we quantitatively compared the spreading dynamics of cells on nanostructured surfaces to those on flat surfaces. Furthermore, we investigated how cells behaved when they simultaneously encountered two topographically distinct surfaces during spreading. This method will allow many exciting opportunities in the fundamental study of cellular dynamics.
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio) and ‡Department of Mechanical Engineering, Pohang University of Science and Technology , San31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
authors.forename: SoonHo
authors.initials: S
authors.lastname: Kweon
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio) and ‡Department of Mechanical Engineering, Pohang University of Science and Technology , San31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Song
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio) and ‡Department of Mechanical Engineering, Pohang University of Science and Technology , San31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
authors.forename: HyoungJun
authors.initials: H
authors.lastname: Park
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio) and ‡Department of Mechanical Engineering, Pohang University of Science and Technology , San31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
authors.forename: Jong-Cheol
authors.initials: JC
authors.lastname: Choi
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio) and ‡Department of Mechanical Engineering, Pohang University of Science and Technology , San31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
mesh_terms: D060833:Cellular Microenvironment; D006367:HeLa Cells; D006801:Humans; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial
keywords: cell migration; cell patterning; cell-friendly photoresist; dynamic patterning; nanostructured surface