title: Role of the durum wheat dehydrin in the function of proteases conferring salinity tolerance in Arabidopsis thaliana transgenic lines.
abstract: Dehydrins are claimed to stabilize macromolecules against freezing damage, dehydration, ionic or osmotic stresses, thermal stress and re-folding yield. However, their precise function remains unknown. In this context, we report the behavior of protease activities in dehydrin transgenic Arabidopsis lines against the wild type plant under salt stress (100mM NaCl). Indeed, proteases play key roles in plants, maintaining strict protein quality control and degrading specific sets of proteins in response to diverse environmental and developmental stimuli. We proved that durum wheat DHN-5 modulates the activity of some proteases, summarized on the promotion of the Cysteinyl protease and the decrease of the Aspartyl protease activity. This fact is also upgraded in salt stress conditions. We conclude that the dehydrin transgenic context encodes salinity tolerance in transgenic lines through the modulation of the interaction not only at transcriptional level but also at protein level and also with the impact of salt stress as an endogenous and exogenous effector on some biocatalysts like proteases.
authors.affiliation: Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, B.P 1177, 3018 Sfax, Tunisia. Electronic address: saibiwalid@gmail.com.
authors.forename: Walid
authors.initials: W
authors.lastname: Saibi
authors.affiliation: Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, B.P 1177, 3018 Sfax, Tunisia.
authors.forename: Nabil
authors.initials: N
authors.lastname: Zouari
authors.affiliation: International Center for Biosaline Agriculture (ICBA), P.O.Box 14660, Dubai, United Arab Emirates.
authors.forename: Khaled
authors.initials: K
authors.lastname: Masmoudi
authors.affiliation: Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, B.P 1177, 3018 Sfax, Tunisia.
authors.forename: Faiçal
authors.initials: F
authors.lastname: Brini
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D004789:Enzyme Activation; D015870:Gene Expression; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant; D012965:Sodium Chloride; D010447:Peptide Hydrolases
keywords: Aspartyl-protease; Cysteinyl-protease; Dehydrin transgenic context; Salinity tolerance; Transgenic Arabidopsis lines and biocatalyst modulation