title: Hemorheological effects of secoisolariciresinol in ovariectomized rats.
abstract: Postmenopausal women often develop hemorheological disorders which may affect the systemic blood circulation and present a cardiovascular risk factor.
  
  
  
  We evaluated effects of secoisolariciresinol (SECO), a phytoestrogen, on hemorheological parameters and lipid peroxidation in a model of the age-related and/or surgical menopause induced by ovariectomy in rats.
  
  
  
  Arterial blood was sampled from sham-operated female rats, ovariectomized rats (OVX), and OVX treated with SECO (OVXSECO) (20 mg/kg/day intragastrically for two weeks). Plasma estrogen concentration and the following hemorheological parameters were measured: RBC aggregation (half-time of aggregation, T1/2; amplitude of aggregation, AMP; aggregation index, AI), RBC deformability (elongation index, EI), whole blood viscosity at the shear rate of 3-300 s-1, plasma viscosity, hematocrit, plasma fibrinogen. Lipid peroxidation was evaluated by measuring conjugated dienes (CD) and thiobarbituric acid reactive substances (TBARS) in plasma.
  
  
  
  Ovariectomy in rats caused a 60% decrease in plasma estrogen level and triggered the development of macro- and microhemorheological abnormalities. Blood viscosity increased by 12-31%, RBC elongation index reduced by 16-28%, and T1/2 and AI increased by 35% and 29% respectively. The increase in blood viscosity correlated predominantly with reduced RBC deformability. Plasma CD and TBARS were elevated by 47% and 104% respectively. SECO therapy for OVX rats reduced blood viscosity by 9-18% and T1/2 by 32%, and increased EI by 4-17%. SECO therapy disrupted the correlation between blood viscosity and RBC deformability. Lipid peroxidation was significantly inhibited, as shown by the reduction in CD and TBARS plasma concentrations by 89% and 70% respectively. SECO did not affect plasma viscosity, estrogen or fibrinogen levels.
  
  
  
  SECO treatment for OVX rats improves blood macro- and microrheological parameters, possibly through antioxidant protection of RBC.
authors.affiliation: Steward St. Elizabeth's Medical Center/Department of Anesthesiology, Critical Care and Pain Medicine, Tufts University School of Medicine, Boston, MA, USA. E-mail: mikhail.maslov@steward.org.
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Maslov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Plotnikova
authors.affiliation: E.D. Goldberg Institute of Pharmacology and Regenerative Medicine, Russian Academy of Sciences, Tomsk, Russia.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Anishchenko
authors.affiliation: E.D. Goldberg Institute of Pharmacology and Regenerative Medicine, Russian Academy of Sciences, Tomsk, Russia.
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Aliev
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Nikolay E
authors.initials: NE
authors.lastname: Nifantiev
authors.affiliation: E.D. Goldberg Institute of Pharmacology and Regenerative Medicine, Russian Academy of Sciences, Tomsk, Russia.
authors.forename: Mark B
authors.initials: MB
authors.lastname: Plotnikov
mesh_terms: D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects*; D002072:Butylene Glycols / Q000494:pharmacology*; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004958:Estradiol / Q000097:blood; D005260:Female; D006400:Hematocrit; D018056:Hemorheology / Q000187:drug effects*; D017705:Lignans / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D010052:Ovariectomy / Q000009:adverse effects*; D010053:Ovary / Q000601:surgery; D048789:Phytoestrogens / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002072:Butylene Glycols; D017705:Lignans; D048789:Phytoestrogens; D004958:Estradiol; C060283:secoisolariciresinol
keywords: Ovariectomized rats; RBC aggregation; RBC deformability; plasma viscosity; secoisolariciresinol; whole blood viscosity