title: Monocytic Angiotensin and Endothelin Receptor Imbalance Modulate Secretion of the Profibrotic Chemokine Ligand 18.
abstract: OBJECTIVE
  To assess monocytic expression and ratio of angiotensin and endothelin receptors in systemic sclerosis (SSc) and their functional relevance.
  
  
  METHODS
  Receptor expression was measured by flow cytometry. Chemokine ligand 18 (CCL18) concentration in supernatants of peripheral blood mononuclear cells stimulated with immunoglobulin G was measured by ELISA.
  
  
  RESULTS
  Monocytes of patients with SSc presented an increased angiotensin II Type 1 receptor (AT1R)/AT2R ratio compared with those of healthy donors. Patients with lung fibrosis and patients with high modified Rodnan skin score showed a reduced endothelin 1 Type A receptor (ETAR)/ETBR ratio. High AT1R/AT2R, but low ETAR/ETBR ratios corresponded to higher CCL18 secretion.
  
  
  CONCLUSIONS
  Altered angiotensin and endothelin receptor ratios observed in SSc influence autoantibody-mediated effects such as secretion of profibrotic CCL18.
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
authors.forename: Judith
authors.initials: J
authors.lastname: Rademacher
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
authors.forename: Angela
authors.initials: A
authors.lastname: Kill
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Mattat
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
authors.forename: Duska
authors.initials: D
authors.lastname: Dragun
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
authors.forename: Elise
authors.initials: E
authors.lastname: Siegert
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck.
authors.forename: Jeannine
authors.initials: J
authors.lastname: Günther
authors.affiliation: From the Department of Rheumatology and Clinical Immunology, and the Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; Cell Autoimmunity, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin; Department of Rheumatology, University of Lübeck, Lübeck, Germany.J. Rademacher, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; A. Kill, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; K. Mattat, Department of Rheumatology and Clinical Immunology, Charité University Hospital; D. Dragun, Professor, Department of Nephrology, Transplantology and Intensive Care, University Hospital Charité; E. Siegert, MD, Department of Rheumatology and Clinical Immunology, Charité University Hospital; J. Günther*, MSc, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ; G. Riemekasten*, Professor, Department of Rheumatology and Clinical Immunology, Charité University Hospital, and Cell Autoimmunity, DRFZ, and Department of Rheumatology, University of Lübeck. Gabriela.Riemekasten@uksh.de.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Riemekasten
mesh_terms: D000328:Adult; D000368:Aged; D019742:Chemokines, CC / Q000097:blood*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D044022:Receptor, Endothelin A / Q000378:metabolism*; D012595:Scleroderma, Systemic / Q000378:metabolism*; D055815:Young Adult
chemical_list: C111792:CCL18 protein, human; D019742:Chemokines, CC; D044140:Receptor, Angiotensin, Type 1; D044022:Receptor, Endothelin A
keywords: ANGIOTENSIN RECEPTORS; AUTOANTIBODIES; ENDOTHELIN RECEPTORS; HUMAN CCL18 PROTEIN; MONOCYTES; SYSTEMIC SCLERODERMA