title: Understanding regulation of the host-mediated gut symbiont population and the symbiont-mediated host immunity in the Riptortus-Burkholderia symbiosis system.
abstract: Valuable insect models have tremendously contributed to our understanding of innate immunity and symbiosis. Bean bug, Riptortus pedestris, is a useful insect symbiosis model due to harboring cultivable monospecific gut symbiont, genus Burkholderia. Bean bug is a hemimetabolous insect whose immunity is not well-understood. However, we recently identified three major antimicrobial peptides of Riptortus and examined the relationship between gut symbiosis and host immunity. We found that the presence of Burkholderia gut symbiont positively affects Riptortus immunity. From studying host regulation mechanisms of symbiont population, we revealed that the symbiotic Burkholderia cells are much more susceptible to Riptortus immune responses than the cultured cells. We further elucidated that the immune-susceptibility of the Burkholderia gut symbionts is due to the drastic change of bacterial cell envelope. Finally, we show that the immune-susceptible Burkholderia symbionts are able to prosper in host owing to the suppression of immune responses of the symbiotic midgut.
authors.affiliation: Department of Microbiology, Kosin University College of Medicine, Busan 602-703, South Korea.
authors.forename: Jiyeun Kate
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Jun Beom
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Ho Am
authors.initials: HA
authors.lastname: Jang
authors.affiliation: College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Han
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
authors.forename: Takema
authors.initials: T
authors.lastname: Fukatsu
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea. Electronic address: brlee@pusan.ac.kr.
authors.forename: Bok Luel
authors.initials: BL
authors.lastname: Lee
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D001424:Bacterial Infections / Q000276:immunology* / Q000382:microbiology; D019117:Burkholderia / Q000276:immunology*; D002473:Cell Wall / Q000378:metabolism; D004198:Disease Susceptibility; D006430:Hemiptera / Q000276:immunology*; D007113:Immunity, Innate; D007422:Intestines / Q000276:immunology* / Q000382:microbiology; D064307:Microbiota; D023421:Models, Animal; D013559:Symbiosis
chemical_list: D023181:Antimicrobial Cationic Peptides
keywords: Antimicrobial peptide; Burkholderia symbiont; Gut immunity; Innate immunity; Insect symbiosis; Riptortus pedestris