title: 1H NMR-based metabolomics study on the physiological variations during the rat pregnancy process.
abstract: In this study, NMR-based metabolomics in combination with multivariate pattern recognition technologies were employed to evaluate the physiological variations in the Wistar rats' plasma that are induced by pregnancy on the gestational days (GDs) 11, 14, 17 and 20. Untargeted metabolomics analysis revealed some possible mechanism of physiological effects for healthy pregnancies and showed a metabolic trajectory during pregnancy process. The levels of 24 metabolites were found to change significantly throughout pregnancy in maternal plasma. These metabolite changes involved in varied kinds of metabolic pathways including synthesis of biological substances, microbial metabolism in diverse environments, protein digestion and absorption, carbohydrate metabolism, digestion and absorption, mineral absorption, and ATP (Adenosine Triphosphate)-binding cassette transporters (ABC transporters). The substantial cores of all the metabolic pathways are promoting fetal growth and development and regulating maternal physiological state. This work showed relevant metabolic pathways perturbation in the maternal plasma due to normal pregnancy and provided the physical basis of time-dependent metabolic trajectory against which disease-related maternal physiological responses may be better understood in future studies.
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China; Northwest Metabolomics Research Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98109, United States.
authors.forename: Guiping
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China.
authors.forename: Zhishui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China. Electronic address: jianghua.feng@xmu.edu.cn.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Feng
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose; D004734:Energy Metabolism; D005260:Female; D008055:Lipids / Q000097:blood; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D011247:Pregnancy / Q000097:blood*; D066244:Proton Magnetic Resonance Spectroscopy; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D008055:Lipids
keywords: Gestation period; Metabolomics; NMR; Pattern recognition; Physiological variation