title: COMPARATIVE EVALUATION OF SEQUENTIAL INTRAOPERATIVE USE OF WHOLE BLOOD FOLLOWED BY BRILLIANT BLUE VERSUS CONVENTIONAL BRILLIANT BLUE STAINING OF INTERNAL LIMITING MEMBRANE IN MACULAR HOLE SURGERY.
abstract: OBJECTIVE
  To compare the structural and functional outcome of use of autologous heparinized whole blood before staining internal limiting membrane with brilliant blue (BB) versus conventional BB-assisted macular hole surgery.
  
  
  METHODS
  Sixty eyes of 60 patients were randomly divided equally in Group A (BB staining using whole blood) and Group B (conventional BB staining). Clinical assessment and spectral domain optical coherence tomography was done at baseline and 3 weeks, 6 weeks, 16 weeks, and 6 months postoperatively.
  
  
  RESULTS
  Group A eyes had a significantly higher best-corrected visual acuity as compared with Group B postoperatively (P < 0.001, <0.001, 0.004, 0.04 at 3, 6, 16 weeks, and 6 months). Inner segment/outer segment junction continuity was noted in greater number of eyes in Group A compared with Group B (P = 0.02, 0.002, 0.003, and 0.03 at 3, 6, 16 weeks, and 6 months). Eyes in Group A had significantly higher outer foveal thickness at 3 weeks (P = 0.001) and 6 weeks (P < 0.001) compared with Group B.
  
  
  CONCLUSIONS
  Use of whole blood before staining internal limiting membrane with BB causes earlier and better visual rehabilitation postoperatively, which could be attributed to earlier photoreceptor regeneration as evidenced by inner segment/outer segment junction continuity and increase in outer foveal thickness.
authors.affiliation: Guru Nanak Eye Centre, Maulana Azad Medical College, New Delhi, India.
authors.forename: Basudeb
authors.initials: B
authors.lastname: Ghosh
authors.forename: Supriya
authors.initials: S
authors.lastname: Arora
authors.forename: Neha
authors.initials: N
authors.lastname: Goel
authors.forename: Anisha
authors.initials: A
authors.lastname: Seth
authors.forename: Richa
authors.initials: R
authors.lastname: Pyare
authors.forename: Preethi
authors.initials: P
authors.lastname: Sridharan
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Thakar
mesh_terms: D000328:Adult; D000368:Aged; D001485:Basement Membrane / Q000473:pathology*; D001557:Benzenesulfonates / Q000008:administration & dosage*; D001769:Blood; D004396:Coloring Agents / Q000008:administration & dosage*; D058450:Endotamponade; D019773:Epiretinal Membrane / Q000473:pathology*; D005260:Female; D006801:Humans; D007429:Intraocular Pressure; D007430:Intraoperative Care; D008297:Male; D008875:Middle Aged; D016684:Prone Position; D012167:Retinal Perforations / Q000503:physiopathology / Q000601:surgery*; D013194:Staining and Labeling / Q000379:methods; D041623:Tomography, Optical Coherence; D014792:Visual Acuity / Q000502:physiology
chemical_list: D001557:Benzenesulfonates; D004396:Coloring Agents; C006796:brilliant blue