title: The Influence of Dexmedetomidine on Ultrasound-induced Pulmonary Capillary Hemorrhage in Rats.
abstract: The use of xylazine, a veterinary sedative, with ketamine for rat anesthesia has been shown to enhance the pulmonary capillary hemorrhage (PCH) effect of diagnostic ultrasound. This study was undertaken to assess whether the sedative/analgesic dexmedetomidine, commonly used in the intensive care unit, can also enhance ultrasound-induced PCH. Female Sprague Dawley rats were anesthetized with various combinations of ketamine plus xylazine or dexmedetomidine. The dosage of dexmedetomidine was reduced for some groups to doses relevant to human clinical usage. The right thorax of all rats was shaved and depilated for ultrasound transmission and the rats were scanned with diagnostic ultrasound using a 7.6-MHz linear array in a 38°C de-gassed water bath. There was no significant difference in PCH results for the recommended anesthetic dosages of ketamine plus xylazine and ketamine plus 500 μg/kg dexmedetomidine. The varied doses of dexmedetomidine enhanced the PCH, even for the lowest dose of 4 μg/kg, equivalent to a low human dose of 0.64 μg/kg. There was no significant difference in PCH for 500 μg/kg dexmedetomidine with or without ketamine. Further research is needed to identify and characterize other factors that may modify the patient risk from ultrasound-induced PCH.
authors.affiliation: Department of Radiology, University of Michigan, Ann Arbor, MI. Electronic address: douglm@umich.edu.
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Miller
authors.affiliation: Department of Radiology, University of Michigan, Ann Arbor, MI.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Dou
authors.affiliation: Department of Radiology, University of Michigan, Ann Arbor, MI.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor, MI.
authors.forename: Krishnan
authors.initials: K
authors.lastname: Raghavendran
mesh_terms: D000818:Animals; D002196:Capillaries / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000528:radiation effects*; D020927:Dexmedetomidine / Q000008:administration & dosage / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006470:Hemorrhage / Q000175:diagnosis / Q000209:etiology*; D006993:Hypnotics and Sedatives / Q000008:administration & dosage / Q000009:adverse effects; D008171:Lung Diseases / Q000175:diagnosis / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000069453:Ultrasonic Waves / Q000009:adverse effects; D014463:Ultrasonography / Q000009:adverse effects*
chemical_list: D006993:Hypnotics and Sedatives; D020927:Dexmedetomidine
keywords: Comet-tail artifact; Dexmedetomidine anesthesia; Mechanical index; Pulmonary ultrasound