title: Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression.
abstract: Mineralizing osteoblasts (MOBs) can release exosomes, although the functional significance remains unclear. In the present study, we demonstrate that exosomes derived from mineralizing pre-osteoblast MC3T3-E1 cells can promote bone marrow stromal cell (ST2) differentiation to osteoblasts. We reveal that MOB-derived exosomes significantly influence miRNA profiles in recipient ST2 cells, and these changes tend to activate the Wnt signaling pathway by inhibiting Axin1 expression and increasing β-catenin expression. We also suggest that MOB derived-exosomes partly induce the variation in miRNA expression in recipient ST2 cells by exosomal miRNA transfer. These findings suggest an exosome-mediated mode of cell-to-cell communication in the osteogenic microenvironment, and also indicate the potential of MOB exosomes in bone tissue engineering.
authors.affiliation: Shandong Academy of Medical Sciences, Shandong Medical Biotechnological Center, Key Laboratory for Biotechnology Drugs of the Ministry of Health, Jinan, China.
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Shandong Academy of Medical Sciences, Shandong Medical Biotechnological Center, Key Laboratory for Biotechnology Drugs of the Ministry of Health, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luan
authors.affiliation: Shandong Academy of Medical Sciences, Shandong Medical Biotechnological Center, Key Laboratory for Biotechnology Drugs of the Ministry of Health, Jinan, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
authors.affiliation: Shandong Academy of Medical Sciences, Shandong Medical Biotechnological Center, Key Laboratory for Biotechnology Drugs of the Ministry of Health, Jinan, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Shandong Academy of Medical Sciences, Shandong Medical Biotechnological Center, Key Laboratory for Biotechnology Drugs of the Ministry of Health, Jinan, China.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Han
mesh_terms: D016475:3T3 Cells; D000818:Animals; D060466:Axin Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic; D002450:Cell Communication; D002460:Cell Line; D060833:Cellular Microenvironment; D019295:Computational Biology; D055354:Exosomes / Q000378:metabolism*; D005103:Expert Systems; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000819:agonists / Q000235:genetics / Q000378:metabolism
chemical_list: D060466:Axin Protein; C555452:Axin1 protein, mouse; D015415:Biomarkers; C495267:CTNNB1 protein, mouse; D035683:MicroRNAs; D051176:beta Catenin
keywords: cell-to-cell communication; exosomes; microRNA; osteoblast differentiation