title: Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis-mass spectrometry.
abstract: A newly developed sheathless interface for capillary electrophoresis-mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine modifications. The system performed well in identification and quantification of both 5-methylcytosine and 5-hydroxymethylcytosine using only 125 pg (∼20 cells) genomic DNA samples.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
authors.forename: Ji
authors.initials: J
authors.lastname: Nie
authors.affiliation: Shanghai AB Sciex Analytical Instrument Trading Co., Ltd, Beijing, P. R. China.
authors.forename: Hong-Xu
authors.initials: HX
authors.lastname: Chen
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
authors.forename: Ying-Lin
authors.initials: YL
authors.lastname: Zhou
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis* / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D023281:Genomics; D057230:Limit of Detection; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA