title: Down-regulation of miR-320 associated with cancer progression and cell apoptosis via targeting Mcl-1 in cervical cancer.
abstract: Our previous studies have demonstrated overexpression of Mcl-1 in cervical cancer tumorigenesis. However, the molecular mechanism of its overexpression remains not elucidated. MiR-320 has been reported to be down-regulated in various types of cancer, and bioinformatics prediction indicated that it may regulate the expression of Mcl-1. The aim of this study is to investigate the role of miR-320 and its target gene Mcl-1 in cervical cancer progression and to assess their clinical significance. miR-320 and Mcl-1 expressions in human cervical cancer tissues were investigated by qRT-PCR, in situ hybridization, and immunohistochemical staining, respectively. The clinicopathological implications of these molecules were analyzed. Bioinformatic prediction and luciferase assays were employed to identify the predicted microRNA (miRNA) which regulates Mcl-1. The apoptosis, proliferation, migration, and invasion assays were performed to investigate the effect of miR-320 on the cervical cancer cells. MiR-320 expression is significantly down-regulated versus Mcl-1 expression is up-regulated in cervical cancer tissues compared with normal controls with a negative correlation between them. Luciferase assay showed that miR-320 negatively regulates Mcl-1 expression. In addition, miR-320 induces apoptosis via down-regulation of Mcl-1 and activation of caspase-3 but inhibits cell proliferation, migration, invasion, and tumorigenesis in cervical cancer cells. Our studies show that miR-320 expression is decreased in cervical cancer, and its expression is negatively correlated with Mcl-1 expression in cervical cancer. In addition, miR-320 inhibits cervical cancer progression by down-regulation of Mcl-1. These results indicate that miR-320 may be an important biomarker and target for diagnosis and treatment of cervical cancer patient.
authors.affiliation: Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, China. zhangting040715@163.com.|Department of Molecular Cell Biology and Toxicology, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment Cancer Center; School of Public Health, Nanjing Medical University, Nanjing, 210029, China. zhangting040715@163.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
authors.affiliation: Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Wang
authors.affiliation: Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Daozhen
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment Cancer Center; School of Public Health, Nanjing Medical University, Nanjing, 210029, China. jwzhou@njmu.edu.cn.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000469:parasitology*
chemical_list: C579095:MCL1 protein, human; C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D053148:Caspase 3
keywords: Apoptosis; Cervical cancer; Mcl-1; miR-320