title: Impacts of dietary calcium, phytate, and nonphytate phosphorus concentrations in the presence or absence of phytase on inositol hexakisphosphate (IP6) degradation in different segments of broilers digestive tract.
abstract: A total of 1,440 straight-run Heritage 56M × fast-feathering Cobb 500F broiler birds were fed from 11 to 13 d of age to determine the impacts of calcium (Ca), phytate phosphorus (PP), nonphytate P (NPP) and phytase concentrations on the myo-inositol hexakisphosphate (IP6) flow through the different parts of gastrointestinal tract (GIT). The experiment was a 2×2×2×3 randomized block design with 2 Ca (0.7 and 1.0%), 2 PP (0.23 and 0.34%), 2 nPP (0.28 and 0.45%) and 3 phytase (0-, 500-, and 1,000-phytase unit (FTU)/kg) concentrations. The experiment was replicated twice (block) with 3 replicates per treatment (TRT) of 10 birds per block. Concentration of IP6 in crop, proventriculus (PROV) plus (+) gizzard (GIZ) and distal ileum digesta as well as the ileal IP6 disappearance was determined at 13 d of age. In crop, higher IP6 concentration was seen with increased Ca (P < 0.05). Despite the interaction between PP and phytase, higher dietary PP led to greater IP6 concentration (P < 0.05). Similar main effects of PP and phytase were also seen in Prov+Giz and ileum (P < 0.05) without interactions. Interaction between Ca and nPP on IP6 concentration was seen in Prov+Giz (P < 0.05). Decreased ileal IP6 disappearance was found at higher Ca (62.3% at 0.7% Ca vs. 57.5% at 1.0% Ca; P < 0.05). In general, adding phytase improved IP6 degradation but the degree of impact was dependent on nPP and PP (P < 0.05). In conclusion, phytase inclusion significantly reduced IP6 concentration and IP6 disappearance in distal ileum regardless of GIT segments or diet composition, but impacts of dietary Ca, nPP, and PP differed depending on GIT segment examined.
authors.affiliation: Department of Animal and Avian Sciences, University of Maryland, College Park, MD.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Animal and Avian Sciences, University of Maryland, College Park, MD rangel@umd.edu.
authors.forename: R
authors.initials: R
authors.lastname: Angel
authors.affiliation: Department of Animal and Avian Sciences, University of Maryland, College Park, MD.
authors.forename: S-W
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Animal and Avian Sciences, University of Maryland, College Park, MD.
authors.forename: K
authors.initials: K
authors.lastname: Brady
authors.affiliation: Enzyme R&D, DuPont Industrial Biosciences, Aarhus, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Yu
authors.affiliation: Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough, UK.
authors.forename: P W
authors.initials: PW
authors.lastname: Plumstead
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002136:Calcium, Dietary / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D041981:Gastrointestinal Tract / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism*; D009928:Organ Specificity; D016226:Phosphorus, Dietary / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D011897:Random Allocation
chemical_list: D002136:Calcium, Dietary; D007295:Inositol Phosphates; D016226:Phosphorus, Dietary; D010833:Phytic Acid; D010832:6-Phytase
keywords: IP6 concentration; crop; ileum; phytase; proventriculus and gizzard