title: Evaluation of Autologous Fascia Implantation With Controlled Release of Fibroblast Growth Factor for Recurrent Laryngeal Nerve Paralysis Due to Long-term Denervation.
abstract: OBJECTIVE
  Paralyzed tissue due to long-term denervation is resistant to many treatments because it induces irreversible histological changes and disorders of deglutition or phonation. We sought to determine the effect of autologous transplantation of fascia into the vocal fold (ATFV) with controlled release of basic fibroblast growth factor (bFGF) on long-term unilateral vocal fold paralysis (UVFP).
  
  
  METHODS
  Unilateral recurrent laryngeal nerve (RLN) section was performed on 20 rats. Five rats were implanted with autologous fascia only (fascia group), and 10 rats were implanted with autologous fascia and a gelatin hydrogel sheet with 1 μg (1 μg bFGF + fascia group) or 0.1 μg (0.1 μg bFGF + fascia group) of bFGF 4 months after RLN section. We evaluated the normalized glottal gap and laryngeal volume and histological changes 3 months after implantation.
  
  
  RESULTS
  The normalized glottal gap was significantly reduced in the 3 fascia implantation groups. Normalized laryngeal volume, fat volume, and lateral thyroarytenoid muscle volume were significantly increased in the 2 fascia implantation with bFGF groups.
  
  
  CONCLUSIONS
  The ATFV with controlled release of bFGF repaired the glottal gap and laryngeal volume after RLN section and may reduce the occurrence of aspiration and hoarseness. We speculate that this treatment improves laryngeal function in long-term RLN denervation.
authors.affiliation: Department of Otolaryngology, Yamato Municipal Hospital, Yamato City, Kanagawa, Japan hiromi.nagai@yamatocity-hosp.jp.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Nishiyama ENT Clinic, Kanagawa, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Nishiyama
authors.affiliation: Department of Otolaryngology Head-Neck Surgery, Kitasato University, Kanagawa, Japan.
authors.forename: Yutomo
authors.initials: Y
authors.lastname: Seino
authors.affiliation: Kyoto University Institute for Frontier Medical Science, Kyoto, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.affiliation: Department of Otolaryngology Head-Neck Surgery, Kitasato University, Kanagawa, Japan.
authors.forename: Makito
authors.initials: M
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D003692:Delayed-Action Preparations; D005205:Fascia / Q000637:transplantation*; D005346:Fibroblast Growth Factors / Q000008:administration & dosage* / Q000494:pharmacology; D020100:Hydrogels; D007821:Laryngeal Muscles / Q000187:drug effects / Q000294:innervation / Q000473:pathology; D007830:Larynx / Q000187:drug effects / Q000473:pathology; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012009:Recurrent Laryngeal Nerve / Q000601:surgery*; D014182:Transplantation, Autologous; D014826:Vocal Cord Paralysis / Q000628:therapy*; D014827:Vocal Cords / Q000187:drug effects / Q000294:innervation / Q000601:surgery*
chemical_list: D003692:Delayed-Action Preparations; D020100:Hydrogels; D005346:Fibroblast Growth Factors
keywords: basic fibroblast growth factor; laryngeal functional disorders; larynx; tissue regeneration; vocal fold paralysis