title: Virus and calcium: an unexpected tandem to optimize insecticide efficacy.
abstract: The effective control of insect pests is based on the rational use of the most efficient and safe insecticide treatments. To increase the effects of classical insecticides and to avoid the ability of certain pest insects to develop resistance, it is essential to propose novel strategies. Previous studies have shown that calcium-dependent phosphorylation/dephosphorylation is now considered as a new cellular mechanism for increasing the target sensitivity to insecticides. Because it is known that virus entry is correlated with intracellular calcium concentration rise, this report attempts to present the most important data relevant to the feasibility of combining an insect virus such as baculovirus or densovirus with an insecticide. In this case, the insect virus is not used as a bioinsecticide but acts as a synergistic agent able to trigger calcium rise and to activate calcium-dependent intracellular signalling pathways involved in the increase of the membrane receptors and/or ion channels sensitivity to insecticides. This virus-insecticide mixture represents a promising alternative to optimize the efficacy of insecticides against insect pests while reducing the doses.
authors.affiliation: Laboratoire RCIM, UPRES EA 2647/USC INRA 1330, SFR 4207 QUASAV, UFR Sciences, Université d'Angers, 2 boulevard Lavoisier, F-49045, Angers, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Apaire-Marchais
authors.affiliation: DGIMI UMR 1333 INRA UM2, Place Eugène Bataillon, cc101, 34095, Montpellier, France.
authors.forename: Mylène
authors.initials: M
authors.lastname: Ogliastro
authors.affiliation: UMR MIVEGEC (IRD 224, CNRS 5290, UM), 911 Avenue Agropolis BP 64501, 34394, Montpellier, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chandre
authors.affiliation: UMR MIVEGEC (IRD 224, CNRS 5290, UM), 911 Avenue Agropolis BP 64501, 34394, Montpellier, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Pennetier
authors.affiliation: Laboratoire RCIM, UPRES EA 2647/USC INRA 1330, SFR 4207 QUASAV, UFR Sciences, Université d'Angers, 2 boulevard Lavoisier, F-49045, Angers, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Raymond
authors.affiliation: Laboratoire RCIM, UPRES EA 2647/USC INRA 1330, SFR 4207 QUASAV, UFR Sciences, Université d'Angers, 2 boulevard Lavoisier, F-49045, Angers, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Lapied
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000254:growth & development*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D017990:Densovirus / Q000254:growth & development*; D007300:Insect Control / Q000379:methods*; D007313:Insecta / Q000187:drug effects* / Q000821:virology*; D007306:Insecticides / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D002118:Calcium