title: Perivascular tissue mediated relaxation - a novel player in human vascular tone regulation.
abstract: Perivascular tissue (PVT) modulates vascular tone, releasing adventitia/adipocyte derived relaxing factor (ADRF). Its physiological role remains unclear. We studied isolated internal thoracic artery (ITA) segments obtained from 132 patients subjected to coronary artery bypass grafting. The vessels were skeletonized in vitro and the ITA rings and PVT were incubated in separate isolated organ baths. Skeletonized ITA segments were first precontracted with 10(-5.5)mol/L 5-hydroxytryptamine hydrochloride. The PVT was next transferred to the ITA tissue bath. This resulted in relaxation of ITA, presumably related to ADRF release from PVT which was floating freely in the tissue bath. The in-vitro relaxation responses were then correlated to patients' characteristics - including demographics, clinical and laboratory data, as well as therapy. Perivascular tissue transfer resulted in 49.7 ± 26.2% relaxation of precontracted ITA segments. In multiple linear regression modelling, the relaxation of ITAto PVT was negatively related to patient age (β = -0.67; 95% CI -1.17 - -0.17; P = 0.009), symptoms of CCS class 4 angina (β = -20.11; 95%CI -32.25 - -7.97; P = 0.001), and positively to body mass (β = 0.37; 95%CI 0.08 - 0.67; P = 0.01) and lack of heart failure symptoms (NYHA class 1) (β = 9.06; 95%CI 0.33 - 17.79; P = 0.04). The relaxation response to PVT was not related to patients' sex, diabetes, hypertension, lipid profile or therapy in both univariate and multivariate analysis. PVT might play an important role in regulating vascular tone in humans as exemplified by its changing physiological function with age and in atherosclerosis.
authors.affiliation: Department of Cardiac Surgery, Medical University of Silesia, School of Medicine in Katowice, Poland. mdeja@sum.edu.pl.
authors.forename: M A
authors.initials: MA
authors.lastname: Deja
authors.affiliation: Department of Cardiac Surgery, Medical University of Silesia, School of Medicine in Katowice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Malinowski
authors.affiliation: Department of Electrocardiology and Heart Failure, Medical University of Silesia, School of Health Sciences in Katowice, Poland.
authors.forename: K S
authors.initials: KS
authors.lastname: Golba
authors.affiliation: Department of Cardiac Surgery, Medical University of Silesia, School of Medicine in Katowice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Piekarska
authors.affiliation: Department of Cardiac Surgery, Medical University of Silesia, School of Medicine in Katowice, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Wos
mesh_terms: D000368:Aged; D003238:Connective Tissue / Q000502:physiology*; D001026:Coronary Artery Bypass; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008323:Mammary Arteries / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D008875:Middle Aged; D012701:Serotonin / Q000494:pharmacology; D019738:Surgically-Created Structures / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology*
chemical_list: D012701:Serotonin