title: The Immunohistochemical Evaluation of Solid Pseudopapillary Tumors of the Pancreas and Pancreatic Neuroendocrine Tumors Reveals ERO1Lβ as a New Biomarker.
abstract: Solid pseudopapillary tumor of the pancreas (SPTP) is a class of low-grade malignant tumors that carry a favorable prognosis after surgery. Our group has reported that dysfunctions in the endoplasmic reticulum (ER) protein processing pathway may play a role in tumor development. However, alterations of this pathway in other pancreatic tumors had not been well investigated. In this study, we collected 35 SPTP and pancreatic neuroendocrine tumor (PNET) specimens and described the clinicopathological features of them. We performed immunohistochemistry (IHC) for 6 representative proteins (ERO1Lβ, TRAM1, GRP94, BIP, P4HB, and PDIA4) involved in the ER pathway in both SPTP and PNET specimens. We compared the IHC scoring results of tumors and matched normal pancreas tissues and demonstrated that these proteins were downregulated in SPTP specimens. Five of these proteins (TRAM1, GRP94, BIP, P4HB, and PDIA4) did not display significant changes between PNET and normal pancreas tissue. However, ERO1Lβ was upregulated in PNET tissues compared to the normal tissues, which could be used as a pathological biomarker in the future.
authors.affiliation: From the Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai (JX, HC, JG, JZ, BS, XD, XZ, CP); Department of Surgery, The Second Affiliated Hospital of Zhejiang University, Hangzhou (YZ); and Research Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China (MS).
authors.forename: Junjie
authors.initials: J
authors.lastname: Xie
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Minmin
authors.initials: M
authors.lastname: Shi
authors.forename: Jiangning
authors.initials: J
authors.lastname: Gu
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Baiyong
authors.initials: B
authors.lastname: Shen
authors.forename: Xiaxing
authors.initials: X
authors.lastname: Deng
authors.forename: Xi
authors.initials: X
authors.lastname: Zhan
authors.forename: Chenghong
authors.initials: C
authors.lastname: Peng
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002648:Child; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D008875:Middle Aged; D018358:Neuroendocrine Tumors / Q000378:metabolism* / Q000473:pathology; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism*; D010179:Pancreas / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C486174:TRAM1 protein, human; C053387:endoplasmin; D011393:Procollagen-Proline Dioxygenase; D050862:Oxidoreductases Acting on Sulfur Group Donors; C485767:ERO1B protein, human; C526497:P4HB protein, human; C526505:PDIA4 protein, human; D019704:Protein Disulfide-Isomerases