title: The LSM1-7 Complex Differentially Regulates Arabidopsis Tolerance to Abiotic Stress Conditions by Promoting Selective mRNA Decapping.
abstract: In eukaryotes, the decapping machinery is highly conserved and plays an essential role in controlling mRNA stability, a key step in the regulation of gene expression. Yet, the role of mRNA decapping in shaping gene expression profiles in response to environmental cues and the operating molecular mechanisms are poorly understood. Here, we provide genetic and molecular evidence that a component of the decapping machinery, the LSM1-7 complex, plays a critical role in plant tolerance to abiotic stresses. Our results demonstrate that, depending on the stress, the complex from Arabidopsis thaliana interacts with different selected stress-inducible transcripts targeting them for decapping and subsequent degradation. This interaction ensures the correct turnover of the target transcripts and, consequently, the appropriate patterns of downstream stress-responsive gene expression that are required for plant adaptation. Remarkably, among the selected target transcripts of the LSM1-7 complex are those encoding NCED3 and NCED5, two key enzymes in abscisic acid (ABA) biosynthesis. We demonstrate that the complex modulates ABA levels in Arabidopsis exposed to cold and high salt by differentially controlling NCED3 and NCED5 mRNA turnover, which represents a new layer of regulation in ABA biosynthesis in response to abiotic stress. Our findings uncover an unanticipated functional plasticity of the mRNA decapping machinery to modulate the relationship between plants and their environment.
authors.affiliation: Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain.
authors.forename: Carlos
authors.identifier: http://orcid.org/0000-0002-9971-4972
authors.initials: C
authors.lastname: Perea-Resa
authors.affiliation: Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain.
authors.forename: Cristian
authors.identifier: http://orcid.org/0000-0001-5652-6595
authors.initials: C
authors.lastname: Carrasco-López
authors.affiliation: Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain.
authors.forename: Rafael
authors.identifier: http://orcid.org/0000-0002-8668-7434
authors.initials: R
authors.lastname: Catalá
authors.affiliation: Laboratory of Growth Regulators, Institute of Experimental Botany Academy of Sciences of the Czech Republic, v.v.i. & Palacký University, 78371 Olomouc, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Turečková
authors.affiliation: Laboratory of Growth Regulators, Institute of Experimental Botany Academy of Sciences of the Czech Republic, v.v.i. & Palacký University, 78371 Olomouc, Czech Republic.
authors.forename: Ondrej
authors.identifier: http://orcid.org/0000-0003-3452-0154
authors.initials: O
authors.lastname: Novak
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah 84112.
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah 84112.
authors.forename: Leslie
authors.identifier: http://orcid.org/0000-0001-6691-2000
authors.initials: L
authors.lastname: Sieburth
authors.affiliation: Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany INRA, Institut Jean-Pierre Bourgin, UMR 1318, 78026 Versailles, France.
authors.forename: José Manuel
authors.identifier: http://orcid.org/0000-0002-5033-7192
authors.initials: JM
authors.lastname: Jiménez-Gómez
authors.affiliation: Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain salinas@cib.csic.es.
authors.forename: Julio
authors.initials: J
authors.lastname: Salinas
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D008954:Models, Biological; D010937:Plant Growth Regulators / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C030052:mRNA decapping enzymes; D012965:Sodium Chloride; D000040:Abscisic Acid; D004722:Endoribonucleases