title: Identification of mumps virus protein and lipid composition by mass spectrometry.
abstract: BACKGROUND
  Mumps virus is a negative-sense, single stranded RNA virus consisting of a ribonucleocapsid core enveloped by a lipid membrane derived from host cell, which causes mumps disease preventable by vaccination. Since virus lipid envelope and glycosylation pattern are not encoded by the virus but dependent on the host cell at least to some extent, the aim of this work was to analyse L-Zagreb (L-Zg) mumps virus lipids and proteins derived from two cell types; Vero and chicken embryo fibroblasts (CEF). Jeryl Lynn 5 (JL5) mumps strain lipids were also analysed.
  
  
  METHODS
  Virus lipids were isolated by organic phase extraction and subjected to 2D-high performance thin layer chromatography followed by lipid extraction and identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Virus samples were also subjected to gel electrophoresis under denaturating conditions and protein bands were excised, in-gel trypsinized and identified by MS as well as tandem MS.
  
  
  RESULTS
  Results showed that lipids of both mumps virus strains derived from Vero cells contained complex glycolipids with up to five monosaccharide units whereas the lipid pattern of mumps virus derived from CEF was less complex. Mumps virus was found to contain expected structural proteins with exception of fusion (F) protein which was not detected but on the other hand, V protein was detected. Most interesting finding related to the mumps proteins is the detection of several forms of nucleoprotein (NP), some of which appear to be C-terminally truncated.
  
  
  CONCLUSIONS
  Differences found in lipid and protein content of mumps virus demonstrated the importance of detailed biochemical characterization of mumps virus and the methodology described here could provide a means for a more comprehensive quality control in vaccine production.
authors.affiliation: University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia. mbrgles@gmail.com.|Center of Excellence for Viral Immunology and Vaccines, CERVirVac, Rijeka, Zagreb, Croatia. mbrgles@gmail.com.
authors.forename: Marija
authors.initials: M
authors.lastname: Brgles
authors.affiliation: Vienna University of Technology, Institute of Chemical Technologies and Analytics, A-1060, Vienna, Austria. maximilian.bonta@tuwien.ac.at.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Bonta
authors.affiliation: University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia. msantak@unizg.hr.|Center of Excellence for Viral Immunology and Vaccines, CERVirVac, Rijeka, Zagreb, Croatia. msantak@unizg.hr.
authors.forename: Maja
authors.initials: M
authors.lastname: Šantak
authors.affiliation: University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia. mmarkusi@unizg.hr.|Center of Excellence for Viral Immunology and Vaccines, CERVirVac, Rijeka, Zagreb, Croatia. mmarkusi@unizg.hr.
authors.forename: Maja
authors.initials: M
authors.lastname: Jagušić
authors.affiliation: University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia. dforcic@unizg.hr.|Center of Excellence for Viral Immunology and Vaccines, CERVirVac, Rijeka, Zagreb, Croatia. dforcic@unizg.hr.
authors.forename: Dubravko
authors.initials: D
authors.lastname: Forčić
authors.affiliation: University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10000, Zagreb, Croatia. bhalassy@unizg.hr.|Center of Excellence for Viral Immunology and Vaccines, CERVirVac, Rijeka, Zagreb, Croatia. bhalassy@unizg.hr.
authors.forename: Beata
authors.initials: B
authors.lastname: Halassy
authors.affiliation: Vienna University of Technology, Institute of Chemical Technologies and Analytics, A-1060, Vienna, Austria. guenter.allmaier@tuwien.ac.at.
authors.forename: Günter
authors.initials: G
authors.lastname: Allmaier
authors.affiliation: Vienna University of Technology, Institute of Chemical Technologies and Analytics, A-1060, Vienna, Austria. martina.marchetti-deschmann@tuwien.ac.at.
authors.forename: Martina
authors.initials: M
authors.lastname: Marchetti-Deschmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000821:virology; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000737:chemistry*; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D008055:Lipids; D014764:Viral Proteins