title: An innovative platform for quick and flexible joining of assorted DNA fragments.
abstract: Successful synthetic biology efforts rely on conceptual and experimental designs in combination with testing of multi-gene constructs. Despite recent progresses, several limitations still hinder the ability to flexibly assemble and collectively share different types of DNA segments. Here, we describe an advanced system for joining DNA fragments from a universal library that automatically maintains open reading frames (ORFs) and does not require linkers, adaptors, sequence homology, amplification or mutation (domestication) of fragments in order to work properly. This system, which is enhanced by a unique buffer formulation, provides unforeseen capabilities for testing, and sharing, complex multi-gene circuitry assembled from different DNA fragments.
authors.affiliation: Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, 37831, USA.
authors.forename: Henrique Cestari
authors.initials: HC
authors.lastname: De Paoli
authors.affiliation: Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, 37831, USA.
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Tuskan
authors.affiliation: Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, 37831, USA.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Yang
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D019175:DNA Methylation; D004262:DNA Restriction Enzymes; D015723:Gene Library; D016366:Open Reading Frames; D015203:Reproducibility of Results
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes