title: A Combinatorial effect of carboxymethyl cellulose based scaffold and microRNA-15b on osteoblast differentiation.
abstract: The present study was aimed to synthesize and characterize a bio-composite scaffold containing carboxymethyl cellulose (CMC), zinc doped nano-hydroxyapatite (Zn-nHAp) and ascorbic acid (AC) for bone tissue engineering applications. The fabricated bio-composite scaffold was characterized by SEM, FT-IR and XRD analyses. The ability of scaffold along with a bioactive molecule, microRNA-15b (miR-15b) for osteo-differentiation at cellular and molecular levels was determined using mouse mesenchymal stem cells (mMSCs). miR-15b acts as posttranscriptional gene regulator and regulates osteoblast differentiation. The scaffold and miR-15b were able to promote osteoblast differentiation; when these treatments were combined together on mMSCs, there was an additive effect on promotion of osteoblast differentiation. Thus, it appears that the combination of CMC/Zn-nHAp/AC scaffold with miR-15b would provide more efficient strategy for treating bone related defects and bone regeneration.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Vimalraj
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Saravanan
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Vairamani
authors.affiliation: Nanotechnology Research Center, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Gopalakrishnan
authors.affiliation: Bioproducts Laboratory, Central Leather Research Institute, Chennai, Tamil Nadu, India.
authors.forename: T P
authors.initials: TP
authors.lastname: Sastry
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India. Electronic address: selvamurugan.n@ktr.srmuniv.ac.in.
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D010006:Osteoblasts / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D035683:MicroRNAs; C570833:Mirn15 microRNA, mouse; D002266:Carboxymethylcellulose Sodium
keywords: Carboxylmethyl cellulose/Zn doped nano-hydroxyapatite/ascorbic acid; Mesenchymal stem cells; miR-15b