title: Morphology and systematics of two freshwater urostylid ciliates, with description of a new species (Protista, Ciliophora, Hypotrichia).
abstract: The morphology of two freshwater urostylid species, Neourostylopsis flava spec. nov. and Pseudourostyla subtropicaChen et al., 2014, isolated from freshwater ponds in northern and southern China, respectively, was investigated following examination of specimens in vivo and following protargol staining. Neourostylopsis flava spec. nov. is distinguished from its congeners by the following characteristics: body size 150-220 × 50-75 μm in vivo; yellow in colour; bright yellow to yellow-brownish spherical cortical granules densely arranged along marginal cirral rows and in irregular short rows on dorsal side; adoral zone with 40-55 membranelles; six to eight frontal, three or four buccal, two pretransverse ventral and seven to nine transverse cirri; 27-40 midventral pairs extending to about anterior 55% of cell; four or five left and four right marginal rows; freshwater habitat. A redescription of a freshwater population of P. subtropica is also provided. Phylogenetic analyses based on small subunit ribosomal DNA sequences shows that P. subtropica and N. flava spec. nov. group with their congeners and both Neourostylopsis and Pseudourostyla are monophyletic.
authors.affiliation: College of Life Science and Technology, Harbin Normal University, Harbin 150025, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Pan
authors.affiliation: Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors.forename: Yangbo
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, China. Electronic address: gaof@ouc.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Al-Farraj
authors.affiliation: Department of Life Sciences, Natural History Museum, London SW7 5BD, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Warren
authors.affiliation: The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: andrews1201@hotmail.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Shao
mesh_terms: D002681:China; D016798:Ciliophora / Q000145:classification* / Q000166:cytology*; D005618:Fresh Water / Q000469:parasitology; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: Freshwater ciliate; Morphology; Neourostylopsis flava spec. nov.; Phylogeny; Pseudourostyla subtropica; SSU rDNA