title: Mini-incision microdissection testicular sperm extraction: a useful technique for men with cryptozoospermia.
abstract: Microdissection testicular sperm extraction (micro-TESE) was developed to minimize the testicular injury associated with multiple open TESEs. We sought to evaluate a mini-incision micro-TESE in men with cryptozoospermia and non-obstructive azoospermia (NOA). We conducted a retrospective study of 26 consecutive men with NOA and cryptozoospermia who underwent a primary (first) micro-TESE between March 2015 and August 2015. Final assessment of sperm recovery (reported on the day of intra-cytoplasmic sperm injection (ICSI)) was recorded as (i) successful (available spermatozoa for ICSI) or (ii) unsuccessful (no spermatozoa for ICSI). The decision to perform a mini-incision micro-TESE (with limited unilateral micro-dissection) or standard/extensive (with unilateral or bilateral micro-dissection) was guided by the intra-operative identification of sperm recovery (≥5 spermatozoa) from the first testicle. Overall, sperm recovery was successful in 77% (20/26) of the men. In 37% of the men (8/26), the mini-incision micro-TESE was successful (positive sperm recovery). The remaining 18 men required a standard (extensive) microdissection: 61% (11/18) underwent a unilateral and 39% (7/18) a bilateral micro-TESE. We found that 90% (9/10) of the men with cryptozoospermia and 63% (10/16) of the men with NOA underwent a unilateral (mini or standard micro-TESE). The mini-incision micro-TESE allowed for successful sperm recovery in 60% (6/10) of the men with cryptozoospermia and 13% (2/16) of the men with NOA. The data demonstrate that a mini-incision micro-TESE together with rapid intra-operative assessment and identification of spermatozoa recovery can be useful in men undergoing microTESE, particularly, men with cryptozoospermia.
authors.affiliation: McGill University, Montreal, QC, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Alrabeeah
authors.affiliation: Ovo Fertility Clinic, Montreal, QC, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Witmer
authors.affiliation: Ovo Fertility Clinic, Montreal, QC, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Ruiz
authors.affiliation: McGill University, Montreal, QC, Canada.
authors.forename: A
authors.initials: A
authors.lastname: AlMalki
authors.affiliation: Ovo Fertility Clinic, Montreal, QC, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Phillips
authors.affiliation: McGill University, Montreal, QC, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Zini
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000601:surgery*; D005240:Feasibility Studies; D006801:Humans; D008297:Male; D042282:Microdissection / Q000379:methods*; D012189:Retrospective Studies; D053625:Sperm Retrieval; D013737:Testis / Q000601:surgery*
keywords: azoospermia; infertility; microsurgery; spermatozoa; testis