title: "Light-up" Sensing of human 8-oxoguanine DNA glycosylase activity by target-induced autocatalytic DNAzyme-generated rolling circle amplification.
abstract: Human 8-oxoguanine DNA glycosylase (hOGG1) plays a crucial role in maintaining the genomic integrity of living organisms for its capability of repairing DNA oxidative damage. The expression level of hOGG1 is closely associated with many diseases including various kinds of cancers. In this study, a novel "light-up" sensor based on target-induced formation of 5' phosphorylated probe and autocatalytic DNAzyme-generated rolling circle amplification has been developed for highly sensitive human 8-oxoguanine DNA glycosylase (hOGG1) activity assay. The approach reaches detection limit as low as 0.001U/mL for hOGG1 via scarcely increased background signal and dual signal amplification strategy. To the best of our knowledge, it is one of the most sensitive methods for the detection of base excision repair enzyme. Moreover, the approach shows excellent specificity over other nonspecific enzymes would interfere with the assay and holds great promise for application in real sample analysis. Hence, the proposed method provides a highly sensitive, selective, and desirable hOGG1 sensing platform.
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Xiang-Juan
authors.initials: XJ
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Cen
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: xiachu@hnu.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Chu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D045647:DNA Glycosylases / Q000032:analysis* / Q000378:metabolism; D021881:DNA, Catalytic / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods; D005470:Fluorometry / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection
chemical_list: D021881:DNA, Catalytic; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human
keywords: Autocatalytic DNAzyme; Rolling circle amplification; Target-induced dual signal amplification strategy; hOGG1