title: [Effect of Bacillus thuringiensis var. israelensis (Bti) on detoxification enzyme activity of larvae of Culex pipiens pallens and Aedes aegypti].
abstract: OBJECTIVE
  To investigate the effect of Bacillus thuringiensis var. israelensis (Bti) on the activities of three detoxification enzymes of Culex pipiens pallens and Aedes aegypti larvae.
  
  
  METHODS
  The activities of glutathione transferase, acetyl cholinesterase and carboxyl esterase, were detected after two kinds of mosquito larvae were treated by Bti at different time and concentrations.
  
  
  RESULTS
  The activities of three detoxification enzymes of the two kinds of mosquito larvae were influenced by Bti treatment. The activity of glutathione transferase was increased after the Bti treatment, but declined significantly and inhibited at a low level sustainably. The activity of carboxyl esterase was increased after the Bti treatment, but recovered to normal level quickly. Acetyl cholinesterase was affected slightly by the Bti treatment, shortly inhibited in the first time and then recovered. The active effects of the three detoxification enzymes were positively related to the concentration of Bti.
  
  
  CONCLUSIONS
  The enzyme activities of glutathione transferase, acetyl cholinesterase and carboxyl esterase could be affected by Bti significantly.
authors.forename: Guang-jie
authors.initials: GJ
authors.lastname: Han
authors.forename: Chuan-ming
authors.initials: CM
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.forename: Jian-hang
authors.initials: JH
authors.lastname: Qi
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000330:Aedes / Q000201:enzymology*; D000818:Animals; D001413:Bacillus thuringiensis; D043182:Carboxylesterase / Q000378:metabolism*; D003465:Culex / Q000201:enzymology*; D005982:Glutathione Transferase / Q000378:metabolism*; D007814:Larva / Q000201:enzymology; D009032:Mosquito Control; D010572:Pest Control, Biological
chemical_list: D005982:Glutathione Transferase; D043182:Carboxylesterase; D000110:Acetylcholinesterase