title: A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse.
abstract: In this study, a co-production of two high value-added products, glucose and xylooligosaccharides (XOS), was investigated by utilizing sugarcane bagasse (SB) within a multi-product bio-refinery framework optimized by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum cellulose conversion of xylan-removed SB, 98.69±1.30%, and a maximum extracted SB xylan conversion into XOS (xylobiose and xylotriose) of 57.36±0.79% that was the highest SB xylan conversion reported in the literature, employing cellulase from Penicillium oxalicum EU2106 and recombinant endo-β-1,4-xylanase in Pichia pastoris. Consequently, a mass balance analysis showed that the maximum yields of glucose and XOS were 34.43±0.32g and 5.96±0.09 g per 100 g raw SB. Overall, this described process may be a preferred option for the comprehensive utilization of SB.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Xue
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yin
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Sui-Xin
authors.initials: SX
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Lin-Hui
authors.initials: LH
authors.lastname: Su
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Duan
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
authors.forename: Jun-Liang
authors.initials: JL
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China. Electronic address: jiaxunfeng@sohu.com.
authors.forename: Jia-Xun
authors.initials: JX
authors.lastname: Feng
mesh_terms: D001709:Biotechnology / Q000379:methods; D002480:Cellulase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D005965:Glucuronates / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D010407:Penicillium / Q000378:metabolism; D010843:Pichia / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry
chemical_list: D004187:Disaccharides; D005965:Glucuronates; D009844:Oligosaccharides; D014312:Trisaccharides; C570991:xylooligosaccharide; C515044:xylotriose; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; C004173:xylobiose; D005947:Glucose
keywords: Box–Behnken design; Co-production of glucose and xylooligosaccharides; High value-added products; Sugarcane bagasse