title: Downregulation of human intercellular adhesion molecule-1 attenuates the metastatic ability in human breast cancer cell lines.
abstract: Intercellular adhesion molecule-1 (ICAM-1) is a cell surface glycoprotein that belongs to immunoglobulin superfamily and plays an important role in tumor cell expansion or metastasis. However, the detailed mechanisms of ICAM-1 in breast cancer remain unclear. In this study, we evaluated the expression level of ICAM-1 in breast cancer using tissue microarray and clinical tissue specimens by immunohistochemical method, and the results revealed that ICAM-1 is highly expressed in the breast cancer tissues. To investigate whether ICAM-1 can affect the metastasis ability in breast cancer, we knocked down ICAM-1 expression in breast cancer cell line MCF-7 by using lentivirus-mediated RNA interference (RNAi). As a result, we stably silenced ICAM-1 expression in MCF-7 cells by infection with lentivirus expressing green fluorescent protein (GFP), the change of metastatic ability of MCF-7 cells was assessed by wound-healing assay, Transwell assay or clone formation assay. Our results showed that silencing of ICAM-1 can inhibit the metastatic ability of MCF-7 cell lines in vitro significantly, and the decreased migration and invasion was accompanied by a reduction of MMP-14. These results implying that ICAM-1 might be involved in the progression of breast cancer metastasis and lentivirus-mediated silencing of ICAM-1 might be a potential therapeutic approach for the treatment of breast cancer.
authors.affiliation: Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Dalin
authors.initials: D
authors.lastname: Di
authors.affiliation: Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Immunology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Ju
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D061986:MCF-7 Cells; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics
chemical_list: D018799:Intercellular Adhesion Molecule-1; D053511:Matrix Metalloproteinase 14