title: The effect of melatonin on oxidative stress and apoptosis in experimental diabetes mellitus-related ovarian injury.
abstract: We aimed to evaluate the effect of melatonin on oxidative stress and ovarian injury in rats. Twenty-four Sprague-Dawley albino rats were divided into three groups: Group 1 as nondiabetic healthy controls (n = 8), group 2 as nontreated diabetic rats (n = 8) and group 3 as melatonin-treated diabetic rats (n = 8). After overt diabetes was produced by intraperitoneal injection of streptozosin, 20 mg/kg/day of melatonin was given intraperitoneally to group 3 for a week. NF-kB and caspase-3 immunoexpressions, lipid peroxidation, the activities of antioxidative enzymes, total oxidant capacity and total antioxidant capacity were assessed. Immunoexpressions of NF-kB and caspase-3 were significantly lower in group 3 than group 2. There was a significant decrease in superoxide dismutase activity in group 2 than group 1 and a significant increase in group 3 compared with group 2. We observed a nonsignificant decrease in catalase activity between group 1 and group 2 and a nonsignificant increase between group 2 and group 3. There was a nonsignificant increase in the plasma level of total oxidant status in group 2 than group 1, but a significant decrease was observed in group 3 compared to group 2. Total antioxidant status was significantly lower in group 2 compared with group 1 and group 3. In conclusion, melatonin ameliorates the negative effects of oxidative stress on DM-related ovarian injury.
authors.affiliation: a Department of Obstetrics and Gynecology .
authors.forename: Umit
authors.initials: U
authors.lastname: Nayki
authors.affiliation: b Department of Anesthesiology and Reanimation .
authors.forename: Didem
authors.initials: D
authors.lastname: Onk
authors.affiliation: c Department of Pathology .
authors.forename: Gurhan
authors.initials: G
authors.lastname: Balci
authors.affiliation: a Department of Obstetrics and Gynecology .
authors.forename: Cenk
authors.initials: C
authors.lastname: Nayki
authors.affiliation: d Department of Cardiovascular Surgery , and.
authors.forename: Alper
authors.initials: A
authors.lastname: Onk
authors.affiliation: e Department of Biochemistry School of Medicine , Erzincan University , Erzincan , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Çankaya
authors.affiliation: e Department of Biochemistry School of Medicine , Erzincan University , Erzincan , Turkey.
authors.forename: Ayse Humeyra
authors.initials: AH
authors.lastname: Taskın Kafa
authors.affiliation: e Department of Biochemistry School of Medicine , Erzincan University , Erzincan , Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kuzucu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D008550:Melatonin / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016328:NF-kappa B; D053148:Caspase 3; D008550:Melatonin
keywords: Apoptosis; NF–kB pathway; diabetes; ovary; oxidative stress; rat