title: A simple and sensitive UHPLC-MS/MS method for quantification of buddlejasaponin IV in rat plasma and its application to a pharmacokinetic study.
abstract: Buddlejasaponin IV (BS-IV), a natural triterpene saponin isolated from several herbal plants, has drawn a lot of attention for its anti-inflammatory, antinociceptive, antihyperlipidemia, and antitumor activities. In this study, a simple and sensitive method for determination of BS-IV in rat plasma was developed for the first time, using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Tenacissoside I was used as an internal standard (IS). Separation was achieved on an Agilent Extend-C18 column with gradient elution using methanol-water as mobile phase at a flow rate of 400μL/min. A triple quadrupole mass spectrometer operating in the positive/negative ion-switching electrospray ionization mode with selection reaction monitoring (SRM) was used to determine BS-IV and IS transitions of 941.4→779.5 and 815.5→755.5, respectively. The lower limit of quantification was 3.00ng/mL with a linear range of 3.0-3000ng/mL. The intra- and inter-day precisions were both ≤10.4% for BS-IV, and the average intra- and inter-day accuracies ranged from -7.2% to 6.7%. The validated assay was successfully applied to a pharmacokinetic study of BS-IV following oral administration of 3, 6, 12mg/kg and an intravenous administration of 0.9mg/kg to rats.
authors.affiliation: Departments of Cardiology and Echocardiography, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Departments of Cardiology and Echocardiography, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Departments of Cardiology and Echocardiography, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Trauma, The First Hospital of Jilin University, Changchun 130021, China. Electronic address: tanl_luk@hotmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*
chemical_list: D012503:Saponins; D014315:Triterpenes; C418227:buddlejasaponin IV
keywords: Buddlejasaponin IV; Pharmacokinetics; Rat plasma; UHPLC-MS/MS