title: Follicle-stimulating hormone-induced rescue of cumulus cell apoptosis and enhanced development ability of buffalo oocytes.
abstract: The effect of follicle-stimulating hormone (FSH) on apoptotic status of cumulus cells, expression of proapoptotic and antiapoptotic genes, and development rate of in vitro fertilization-produced buffalo embryos were investigated. FSH supplementation in in vitro maturation-medium resulted in a dose-dependent reduction in the expression of proapoptotic genes namely, BCL2-associated X protein (BAX), cytochrome c, and caspase-3 and increase in the expression of antiapoptotic genes such as B-cell lymphoma 2 (BCL2) and X-linked inhibitor of apoptosis protein (XIAP) in cumulus cells of mature oocyte. Cumulus expansion, oocyte maturation, cleavage, and blastocyst development rates were significantly higher (P < 0.05) in 5 and 10-μg/mL FSH-supplemented groups as compared with control. Significant increase in the expression of FSH receptor messenger RNA was also found with 5 and 10-μg/mL FSH (P < 0.05). Terminal deoxynucleotidyl transferase dUTP nick end labeling assay confirmed that the population of apoptotic cumulus cells of matured oocytes was reduced in the FSH-treated groups as compared with control (P < 0.05). In conclusion, our data suggest that FSH may attenuate apoptosis in cumulus cells via mitochondria-dependent apoptotic pathway by increasing XIAP expression, resulting in a more favorable ratio of BCL2/BAX expression and decreasing the cytochrome c and caspase-3 expression, eventually contributing to developmental competence of oocytes. The information generated will help in improving the in vitro embryo production program in buffalo.
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: A
authors.initials: A
authors.lastname: Jain
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: T
authors.initials: T
authors.lastname: Jain
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: M
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: S
authors.initials: S
authors.lastname: De
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: M
authors.initials: M
authors.lastname: Gohain
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India. Electronic address: tirthadatta@gmail.com.
authors.forename: T K
authors.initials: TK
authors.lastname: Datta
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002020:Buffaloes / Q000502:physiology*; D054885:Cumulus Cells / Q000187:drug effects* / Q000502:physiology; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D046149:Embryo Culture Techniques / Q000662:veterinary; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology
chemical_list: D005640:Follicle Stimulating Hormone
keywords: Apoptosis; Buffalo; Cumulus cell; FSH