title: Assessment of Pradosia huberi effects on the reproductive system of male rats.
abstract: Pradosia huberi is a species found in the Amazon region and used as an antiulcerogenic and gastroprotective agent; however, phytochemical analysis has revealed the presence of compounds with potential toxic effects on the reproductive system. For the evaluation of the toxicity of P. huberi on male fertility, male Wistar rats were divided into four groups: one control (distilled water p.o.) and three treated (hydroalcoholic extract of the stem bark of P. Huberi (PH-HAE) at doses of 1.22, 6.1, and 30.5 mg/kg p.o.) once daily, for 63 days. In the last week of treatment (from the 57th to the 63rd day), the rats were mated with untreated virgin females (n = 30/group) and were killed on day 64. To investigate the toxic potential of PH-HAE on the reproductive system of rats the following parameters were evaluated: sperm production, genotoxicity, and general development. The production of gametes and their morphology did not differ between control and treated groups. Treatment with PH-HAE did not result in fewer vaginal plugs formed, indicating that the ability to mate was not impaired, but caused an increase of 14.3 and 10.8% in the preimplantation loss index, a reduction of 14.3 and 10.8% in the implantation index, and a reduction of 5.6 and 8.2% in the postimplantation loss index of female rats mated with rats treated with 6.1 and 30.5 mg/kg, respectively, indicating a possible toxic action of PH-HAE on the reproductive system of rats.
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil lanafarma@gmail.com.
authors.forename: Elane
authors.initials: E
authors.lastname: Cristina Silva Dos Santos
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Priscylla Silva
authors.initials: PS
authors.lastname: Antunes
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Flávia Luana Pereira
authors.initials: FL
authors.lastname: Dos Santos
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Aldeíde de Oliveira Batista
authors.initials: Ade O
authors.lastname: Rocha
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: João Carlos Lima Rodrigues
authors.initials: JC
authors.lastname: Pita
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Aline Lira
authors.initials: AL
authors.lastname: Xavier
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Cibério Landim
authors.initials: CL
authors.lastname: Macêdo
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Kerollayne Christtine
authors.initials: KC
authors.lastname: Jacob
authors.affiliation: Biotechnology Center, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Nayara Alves
authors.initials: NA
authors.lastname: de Oliveira
authors.affiliation: Institute of Scientific and Technological Research of Amapa, 68900-260 Macapá, AP, Brazil.
authors.forename: Alessandra Azevedo Nascimento
authors.initials: AA
authors.lastname: de Medeiros
authors.affiliation: Department of Pharmaceutical Sciences, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Margareth de Fátima Formiga Melo
authors.initials: Mde F
authors.lastname: Diniz
authors.affiliation: Department of Physiology and Pathology, Federal University of Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Rita
authors.initials: R
authors.lastname: de Cássia da Silveira E Sá
mesh_terms: D000818:Animals; D005260:Female; D005298:Fertility / Q000187:drug effects; D005837:Genitalia, Male / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000009:adverse effects*; D017208:Rats, Wistar; D029632:Sapotaceae / Q000737:chemistry*; D013091:Spermatogenesis / Q000187:drug effects
chemical_list: D010936:Plant Extracts
keywords: Pradosia huberi; fertility; gametes; rats; reproductive toxicity; sperm