title: Cytotoxic Effects of Phosphonate-Functionalized Mesoporous Silica Nanoparticles.
abstract: In this work, we synthesized pristine mesoporous silica nanoparticles (MSN) and functionalized these with phosphonate groups (MSN-Phos). We report, for the first time, cell death in MCF-7 cells (human breast adenocarcinoma cell line) when exposed to the empty MSN and MSN-Phos nanoparticles. In comparison, the same nanoparticles were found to elicit few deleterious effects on normal human foreskin fibroblast cells (BJ cells). MCF-7 cells were found to exhibit a concentration-dependent uptake, whereas no detectable nanoparticle uptake was observed in the BJ cells, irrespective of treatment dosage. A disruption of the cell cycle in the MCF-7 cells was determined to be the cause of cell death from the nanoparticle exposure, thereby suggesting the role of nondrug loaded MSN and MSN-Phos as effective anticancer drugs.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore , 4 Engineering Drive 4, Singapore 117585, Singapore.
authors.forename: Nandita
authors.initials: N
authors.lastname: Menon
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore , 4 Engineering Drive 4, Singapore 117585, Singapore.
authors.forename: David T
authors.initials: DT
authors.lastname: Leong
mesh_terms: D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D057446:Hydrodynamics; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D063065:Organophosphonates / Q000494:pharmacology*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D063065:Organophosphonates; D012822:Silicon Dioxide
keywords: BJ cells; MCF-7 cells; anticancer drugs; cell cycle; mesoporous silica nanoparticles; phosphonate groups