title: [Development of HTS model on SERT inhibitors combined biological screening model with HTVS].
abstract: In order to improve the efficiency of drug screening on serotonin transporter (SERT) inhibitors, a high-throughput screening (HTS) model is established in RBL-2H3 cells. The RBL-2H3 cells are very similar to the serotonin genetic neuro, in modulation of post-receptor mechanisms and transduction pathway of SERT reactivated. Depending on a fluorescence substrate ASP+ used in detection method of inhibitor rates, it's convenient, quick, accurate and effective, not making the environmental biohazard compared with radioactive experiments. Furthermore, biological screening model combined with computer aided virtual screening technique describing high-throughput virtual screening (HTVS). Bayesian classification method and molecular fingerprint similarity were applied to virtual screening technique, for screening compounds in compound library. Some compounds have been found, and then validated further by biological screening model. Combination of HTS and HTVS improves the efficiency of screening SERT inhibitors.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Jian-song
authors.initials: JS
authors.lastname: Fang
authors.forename: Ai-lin
authors.initials: AL
authors.lastname: Liu
authors.forename: Guan-hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D057166:High-Throughput Screening Assays; D008954:Models, Biological; D051381:Rats; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*
chemical_list: D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors