title: Sensitive Detection of Organophosphorus Pesticides in Medicinal Plants Using Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction Combined with Sweeping Micellar Electrokinetic Chromatography.
abstract: A simple, rapid, and sensitive method using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) combined with sweeping micellar electrokinetic chromatography (sweeping-MEKC) has been developed for the determination of nine organophosphorus pesticides (chlorfenvinphos, parathion, quinalphos, fenitrothion, azinphos-ethyl, parathion-methyl, fensulfothion, methidathion, and paraoxon). The important parameters that affect the UA-DLLME and sweeping efficiency were investigated. Under the optimized conditions, the proposed method provided 779.0-6203.5-fold enrichment of the nine pesticides compared to the normal MEKC method. The limits of detection ranged from 0.002 to 0.008 mg kg(-1). The relative standard deviations of the peak area ranged from 1.2 to 6.5%, indicating the good repeatability of the method. Finally, the developed UA-DLLME-sweeping-MEKC method has been successfully applied to the analysis of the investigated pesticides in several medicinal plants, including Lycium chinense, Dioscorea opposite, Codonopsis pilosula, and Panax ginseng, indicating that this method is suitable for the determination of trace pesticide residues in real samples with complex matrices.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Jin-Chao
authors.initials: JC
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: He
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Yuan-Jia
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macau 999078, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D010573:Pesticide Residues / Q000737:chemistry* / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D009943:Organophosphorus Compounds; D010573:Pesticide Residues
keywords: capillary electrophoresis; organophosphorus; sweeping; ultrasound-assisted dispersive liquid−liquid microextraction