title: Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs.
abstract: Eukaryotes remodel the nucleus during mitosis using a variety of mechanisms that differ in the timing and the extent of nuclear envelope (NE) breakdown. Here, we probe the principles enabling this functional diversity by exploiting the natural divergence in NE management strategies between the related fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus [1-3]. We show that inactivation of Ned1, the phosphatidic acid phosphatase of the lipin family, by CDK phosphorylation is both necessary and sufficient to promote NE expansion required for "closed" mitosis in S. pombe. In contrast, Ned1 is not regulated during division in S. japonicus, thus limiting membrane availability and necessitating NE breakage. Interspecies gene swaps result in phenotypically normal divisions with the S. japonicus lipin acquiring an S. pombe-like mitotic phosphorylation pattern. Our results provide experimental evidence for the mitotic regulation of phosphatidic acid flux and suggest that the regulatory networks governing lipin activity diverged in evolution to give rise to strikingly dissimilar mitotic programs.
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Makarova
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, UK.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240, USA.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240, USA.
authors.forename: Janel Renée
authors.initials: JR
authors.lastname: Beckley
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240, USA.
authors.forename: Kathleen Louise
authors.initials: KL
authors.lastname: Gould
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, UK. Electronic address: snezhana.oliferenko@kcl.ac.uk.
authors.forename: Snezhana
authors.initials: S
authors.lastname: Oliferenko
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D048750:Cell Nucleus Division / Q000502:physiology*; D020090:Chromosome Segregation / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D009930:Organic Chemicals / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D009930:Organic Chemicals; C088310:lipine