title: Fumarate Reductase-Producing Enterococci Reduce Methane Production in Rumen Fermentation In Vitro.
abstract: Biotic agents such as fumarate-reducing bacteria can be used for controlling methane (CH4) production in the rumen. Fumarate-reducing bacteria convert fumarate to succinate by fumarate reductase, ultimately leading to the production of propionate. Fumarate-reducing bacteria in the genus Enterococcus were isolated from rumen fluid samples from slaughtered Korean native goats. The enterococci were identified as Enterococcus faecalis SROD5 and E. faecium SROD by phylogenetic analyses of 16S rRNA gene sequences. The fumarate reductase activities of the SROD5 and SROD strains were 42.13 and 37.05 mM NADH oxidized/min/mg of cellular nitrogen (N), respectively. Supplementation of rumen fermentation in vitro with the SROD5 and SROD strains produced significantly higher propionate, butyrate, and total volatile fatty acid (VFA) concentrations than controls at 12 h; VFA concentrations tended to increase after 24 h of incubation. The generated CH4 concentration was significantly lower in the SROD5 and SROD treatment groups after 24 h of incubation. These findings indicate that E. faecium SROD has potential as a direct-fed microbial additive for increasing total VFAs while decreasing CH4 production in rumen fermentation in vitro.
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Seon-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Lovelia L
authors.initials: LL
authors.lastname: Mamuad
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of Korea.
authors.forename: Dong-Woon
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Environment, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Soo-Ki
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Sang-Suk
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D016983:Enterococcus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005285:Fermentation; D006041:Goats; D008697:Methane / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D013385:Succinate Dehydrogenase; D008697:Methane
keywords: Enterococcus faecalis SROD5; Enterococcus faecium SROD; fumarate reductase activity; methane; rumen fermentation in vitro