title: Electrochemical examination of ability of dsDNA/PAM composites for storing and releasing of doxorubicin.
abstract: Composites consisting of ss- and ds-DNA strands and polyacrylamide (PAM) hydrogel have been synthesized. DNA was entrapped non-covalently. The obtained DNA biomaterial exhibited a strong increase in guanine and adenine anodic currents when temperature reached the physiological level. This increase was related to the unique oligonucleotide structural changes in the composite. The structural alterations in the PAM lattices were employed for the release of the drug accumulated in the composite. Doxorubicin (Dox) was selected as the drug; it was accumulated by intercalation to dsDNA and was slowly released from the dsDNA/PAM system by using a minor temperature increase (up to 40÷45 °C) as it is routinely done in hyperthermia. The applied release temperature was either constant or oscillating. The binding strength, the rate of Dox release and the properties of the composite were examined using voltammetry, SEM and ICP-MS.
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland. Electronic address: ezabost@chem.uw.edu.pl.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Zabost
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Liwinska
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Karbarz
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Eliza
authors.initials: E
authors.lastname: Kurek
authors.affiliation: College of Rehabilitation, Kasprzaka 49, 01-234 Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Lyp
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Mikolaj
authors.initials: M
authors.lastname: Donten
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Stojek
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage*; D006147:Guanine / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007364:Intercalating Agents / Q000008:administration & dosage; D010084:Oxidation-Reduction; D013696:Temperature; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000903:Antibiotics, Antineoplastic; D001672:Biocompatible Materials; D004247:DNA; D003692:Delayed-Action Preparations; D004317:Doxorubicin; D006147:Guanine; D020100:Hydrogels; D007364:Intercalating Agents; D000099166:Polyacrylamides
keywords: DNA electrooxidation; Doxorubicin; Drug delivery system; Polyacrylamide; Square wave voltammetry; Switching system