title: A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp.
abstract: Penaeus monodon densovirus (PmDNV) is one of the major causes of stunted shrimp in the aquaculture industry in Thailand. Significant reductions in levels of PmDNV as assessed by PCR analysis of shrimp hepatopancreas were seen in both prophylactic and curative experiments after feeding shrimp with a formulated diet containing mixed inactivated bacteria harboring dsRNAs corresponding to the PmDNV ns1 and vp genes. Significant reductions of approximately 88% (prophylactic) and 64% (curative) of PmDNV were observed, suggesting that this diet has a high potential for application in commercial aquaculture for reducing PmDNV associated stunted growth of shrimp.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
authors.forename: Chaweewan
authors.initials: C
authors.lastname: Chimwai
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
authors.forename: Punnee
authors.initials: P
authors.lastname: Tongboonsong
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
authors.forename: Orathai
authors.initials: O
authors.lastname: Namramoon
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand; Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Sakol
authors.initials: S
authors.lastname: Panyim
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand. Electronic address: pongsopee.att@mahidol.ac.th.
authors.forename: Pongsopee
authors.initials: P
authors.lastname: Attasart
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D061046:Biological Control Agents; D017990:Densovirus / Q000235:genetics / Q000502:physiology*; D050296:Microbial Viability; D033561:Penaeidae / Q000502:physiology / Q000821:virology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism / Q000494:pharmacology*; D013785:Thailand; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics
chemical_list: D061046:Biological Control Agents; D012330:RNA, Double-Stranded; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins
keywords: Double-stranded RNA; Oral delivery; Penaeus monodon densovirus; PmDNV inhibition; RNAi; Shrimp