title: Genetic Diversity of the Two Commercial Tetraploid Cotton Species in the Gossypium Diversity Reference Set.
abstract: A diversity reference set has been constructed for the Gossypium accessions in the US National Cotton Germplasm Collection to facilitate more extensive evaluation and utilization of accessions held in the Collection. A set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid Gossypium accessions representative of the range of diversity of the improved and wild accessions of G. hirsutum and G. barbadense. The reference set contained 410 G. barbadense accessions and 1523 G. hirsutum accessions. Observed numbers of polymorphic and private bands indicated a greater diversity in G. hirsutum as compared to G. barbadense as well as in wild-type accessions as compared to improved accessions in both species. The markers clearly differentiated the 2 species. Patterns of diversity within species were observed but not clearly delineated, with much overlap occurring between races and regions of origin for wild accessions and between historical and geographic breeding pools for cultivated accessions. Although the percentage of accessions showing introgression was higher among wild accessions than cultivars in both species, the average level of introgression within individual accessions, as indicated by species-specific bands, was much higher in wild accessions of G. hirsutum than in wild accessions of G. barbadense. The average level of introgression within individual accessions was higher in improved G. barbadense cultivars than in G. hirsutum cultivars. This molecular characterization reveals the levels and distributions of genetic diversity that will allow for better exploration and utilization of cotton genetic resources.
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones). Lori.Hinze@ars.usda.gov.
authors.forename: Lori L
authors.initials: LL
authors.lastname: Hinze
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: Elodie
authors.initials: E
authors.lastname: Gazave
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gore
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: David D
authors.initials: DD
authors.lastname: Fang
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: Brian E
authors.initials: BE
authors.lastname: Scheffler
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: John Z
authors.initials: JZ
authors.lastname: Yu
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: Don C
authors.initials: DC
authors.lastname: Jones
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: James
authors.initials: J
authors.lastname: Frelichowski
authors.affiliation: From the USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, College Station, TX (Hinze, Yu, Frelichowski, and Percy); School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY (Gazave and Gore); USDA-ARS, Southern Regional Research Center, Cotton Fiber Bioscience Research Unit, New Orleans, LA (Fang); USDA-ARS, Jamie Whitten Delta States Research Center, Genomics and Bioinformatics Research Unit, Stoneville, MS (Scheffler); and Cotton Incorporated, Cary, NC (Jones).
authors.forename: Richard G
authors.initials: RG
authors.lastname: Percy
mesh_terms: D000483:Alleles; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D003368:Gossypium / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D057891:Tetraploidy
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Gossypium barbadense; Gossypium hirsutum; germplasm collection; introgression; population structure