title: Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression.
abstract: The gaseous phytohormone ethylene participates in the regulation of root growth and development in Arabidopsis. It is known that root growth inhibition by ethylene involves auxin, which is partially mediated by the action of the WEAK ETHYLENE INSENSITIVE2/ANTHRANILATE SYNTHASE α1 (WEI2/ASA1), encoding a rate-limiting enzyme in tryptophan (Trp) biosynthesis, from which auxin is derived. However, the molecular mechanism by which ethylene decreases root growth via ASA1 is not understood. Here we report that the ethylene-responsive AP2 transcription factor, ETHYLENE RESPONSE FACTOR1 (ERF1), plays an important role in primary root elongation of Arabidopsis. Using loss- and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth. This discloses one mechanism linking ethylene signaling and auxin biosynthesis in Arabidopsis roots.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province, China.
authors.forename: Jie-Li
authors.initials: JL
authors.lastname: Mao
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province, China.
authors.forename: Zi-Qing
authors.initials: ZQ
authors.lastname: Miao
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province, China.|Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui Province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province, China.
authors.forename: Lin-Hui
authors.initials: LH
authors.lastname: Yu
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province, China.
authors.forename: Xiao-Teng
authors.initials: XT
authors.lastname: Cai
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui Province, China.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Xiang
mesh_terms: D000878:Anthranilate Synthase / Q000096:biosynthesis* / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D010454:Peptide Termination Factors / Q000096:biosynthesis* / Q000235:genetics; D010937:Plant Growth Regulators / Q000096:biosynthesis* / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D005030:Ethylenes; D007210:Indoleacetic Acids; D010454:Peptide Termination Factors; D010937:Plant Growth Regulators; C494978:eukaryotic release factor 1, Arabidopsis; C036216:ethylene; C543688:ASA1 protein, Arabidopsis; D000878:Anthranilate Synthase