title: Molecular changes in mitochondrial respiratory activity and metabolic enzyme activity in muscle of four pig breeds with distinct metabolic types.
abstract: Skeletal muscles are metabolically active and have market value in meat-producing farm animals. A better understanding of biological pathways affecting energy metabolism in skeletal muscle could advance the science of skeletal muscle. In this study, comparative pathway-focused gene expression profiling in conjunction with muscle fiber typing were analyzed in skeletal muscles from Duroc, Pietrain, and Duroc-Pietrain crossbred pigs. Each breed type displayed a distinct muscle fiber-type composition. Mitochondrial respiratory activity and glycolytic and oxidative enzyme activities were comparable among genotypes, except for significantly lower complex I activity in Pietrain pigs homozygous-positive for malignant hyperthermia syndrome. At the transcriptional level, lactate dehydrogenase B showed breed specificity, with significantly lower expression in Pietrain pigs homozygous-positive for malignant hyperthermia syndrome. A similar mRNA expression pattern was shown for several subunits of oxidative phosphorylation complexes, including complex I, complex II, complex IV, and ATP synthase. Significant correlations were observed between mRNA expression of genes in focused pathways and enzyme activities in a breed-dependent manner. Moreover, expression patterns of pathway-focused genes were well correlated with muscle fiber-type composition. These results stress the importance of regulation of transcriptional rate of genes related to oxidative and glycolytic pathways in the metabolic capacity of muscle fibers. Overall, the results further the breed-specific understanding of the molecular basis of metabolic enzyme activities, which directly impact meat quality.
authors.affiliation: Leibniz Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Leibniz Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Nares
authors.initials: N
authors.lastname: Trakooljul
authors.affiliation: Leibniz Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Eduard
authors.initials: E
authors.lastname: Muráni
authors.affiliation: Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, D-30173, Hannover, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Krischek
authors.affiliation: Insititute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.affiliation: Department of Animal Sciences, Quality of Food of Animal Origin, Georg-August-University Goettingen, Goettingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wicke
authors.affiliation: Leibniz Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.affiliation: Leibniz Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. s.wimmers@fbn-dummertorf.de.
authors.forename: Siriluck
authors.identifier: http://orcid.org/0000-0003-4714-1057
authors.initials: S
authors.lastname: Ponsuksili
mesh_terms: D000818:Animals; D001947:Breeding; D008931:Mitochondria, Muscle / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D013552:Swine
chemical_list: D024101:Mitochondrial Proteins; D009124:Muscle Proteins
keywords: Expression pattern; Metabolic enzymes; Muscle fiber; Pig