title: Transforming growth factor-beta increases breast cancer stem cell population partially through upregulating PMEPA1 expression.
abstract: The prostate transmembrane protein, androgen-induced 1 (PMEPA1) has been previously shown to promote solid malignancies in a variety of cancers, but the role and mechanisms of PMEPA1 in breast cancer has not been fully addressed. Here, we found that PMEPA1 was upregulated in breast cancer cell lines as well as in a set of clinical invasive breast ductal carcinomas. Interestingly, depletion of PMEPA1 decreased breast cancer stem cell (CSC)-enriched populations, while ectopic overexpression of PMEPA1 increased breast CSC-enriched populations. Furthermore, transforming growth factor-β (TGF-β) treatment was also found to upregulate PMEPA1 expression and the CSC-enriched populations in triple-negative breast cancer cell lines. TGF-β-induced PMEPA1 expression partially contributed to TGF-β-induced breast CSC maintenance. These findings suggest that TGF-β-PMEPA1 axis might provide new diagnosis and therapeutic targets for breast cancer treatment.
authors.affiliation: Pharmaceutical College of Kunming Medical University, Kunming 650500, China Department of Neurology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Nie
authors.affiliation: Department of Pathology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
authors.forename: Zhongmei
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
authors.forename: Ceshi
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China wangdianhuakm@126.com liurong@mail.kiz.ac.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Pharmaceutical College of Kunming Medical University, Kunming 650500, China wangdianhuakm@126.com liurong@mail.kiz.ac.cn.
authors.forename: Dianhua
authors.initials: D
authors.lastname: Wang
mesh_terms: D001943:Breast Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D008565:Membrane Proteins; C412868:PMEPA1 protein, human; D016212:Transforming Growth Factor beta
keywords: PMEPA1; TGF-β; cancer stem cell