title: Asanoa endophyticasp. nov., an endophytic actinomycete isolated from the rhizome ofBoesenbergia rotunda.
abstract: A novel Gram-stain-positive, non-motile, endophytic actinomycete, designated strain BR3-1T, which produced spore chains borne on the tips of short sporophores, was isolated from the rhizome ofBoesenbergia rotundacollected from Udon Thani province, Thailand. This strain was investigated for its taxonomic position using a polyphasic approach. The strain contained 3-hydroxydiaminopimelic acid andmeso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars comprised glucose, mannose, rhamnose, ribose and xylose. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides were found as the characteristic phospholipids. The predominant menaquinones were MK-10(H8) and MK-10(H6). The major cellular fatty acids were iso-C16 : 0, iso-C15 : 0and anteiso-C15 : 0. The G+C content of the genomic DNA was 71.4 mol%. 16S rRNA gene sequence analysis revealed that strain BR3-1Tbelonged to the genusAsanoaand was most closely related toAsanoa ishikariensis(99.39 %),Asanoa iriomotensis(99.31 %),Asanoa siamensis(99.17 %),Asanoa ferruginea(98.84 %) andAsanoa hainanensis(98.71 %). The DNA-DNA relatedness value between strain BR3-1Tand its phylogenetically closest relatives was in the range of 15.4 % ± 1.2 to 45.8 % ± 2.6. In addition, some physiological and biochemical properties indicated that strain BR3-1Tcould be readily distinguished from all type strains in the genusAsanoa. Thus, strain BR3-1Tshould be classified as a representative of a novel species, for which the nameAsanoa endophyticasp. nov. is proposed. The type strain is BR3-1T( = BCC 66355T= NBRC 110002T).
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang,Bangkok 10520,Thailand.
authors.forename: Nantawan
authors.initials: N
authors.lastname: Niemhom
authors.affiliation: Bioassay Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang,Pathum Thani 12120,Thailand.
authors.forename: Chanikul
authors.initials: C
authors.lastname: Chutrakul
authors.affiliation: BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang,Pathum Thani 12120,Thailand.
authors.forename: Chanwit
authors.initials: C
authors.lastname: Suriyachadkun
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang,Bangkok 10520,Thailand.|Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang,Bangkok 10520,Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai