title: MATE Transporter-Dependent Export of Hydroxycinnamic Acid Amides.
abstract: The ability of Arabidopsis thaliana to successfully prevent colonization by Phytophthora infestans, the causal agent of late blight disease of potato (Solanum tuberosum), depends on multilayered defense responses. To address the role of surface-localized secondary metabolites for entry control, droplets of a P. infestans zoospore suspension, incubated on Arabidopsis leaves, were subjected to untargeted metabolite profiling. The hydroxycinnamic acid amide coumaroylagmatine was among the metabolites secreted into the inoculum. In vitro assays revealed an inhibitory activity of coumaroylagmatine on P. infestans spore germination. Mutant analyses suggested a requirement of the p-coumaroyl-CoA:agmatine N4-p-coumaroyl transferase ACT for the biosynthesis and of the MATE transporter DTX18 for the extracellular accumulation of coumaroylagmatine. The host plant potato is not able to efficiently secrete coumaroylagmatine. This inability is overcome in transgenic potato plants expressing the two Arabidopsis genes ACT and DTX18. These plants secrete agmatine and putrescine conjugates to high levels, indicating that DTX18 is a hydroxycinnamic acid amide transporter with a distinct specificity. The export of hydroxycinnamic acid amides correlates with a decreased ability of P. infestans spores to germinate, suggesting a contribution of secreted antimicrobial compounds to pathogen defense at the leaf surface.
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
authors.forename: Melanie
authors.identifier: http://orcid.org/0000-0002-8136-9250
authors.initials: M
authors.lastname: Dobritzsch
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
authors.forename: Tilo
authors.identifier: http://orcid.org/0000-0003-3383-9518
authors.initials: T
authors.lastname: Lübken
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
authors.forename: Lennart
authors.initials: L
authors.lastname: Eschen-Lippold
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Gorzolka
authors.affiliation: Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany Institute of Pharmacy, Biogenic Drugs, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
authors.forename: Elke
authors.identifier: http://orcid.org/0000-0003-3803-1614
authors.initials: E
authors.lastname: Blum
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Matern
authors.affiliation: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
authors.forename: Sylvestre
authors.initials: S
authors.lastname: Marillonnet
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Böttcher
authors.affiliation: Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany Institute of Pharmacy, Biogenic Drugs, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
authors.forename: Birgit
authors.identifier: http://orcid.org/0000-0002-0615-5674
authors.initials: B
authors.lastname: Dräger
authors.affiliation: Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany Interdisciplinary Centre for Crop Plant Research, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany srosahl@ipb-halle.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Rosahl
mesh_terms: D000577:Amides / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D003373:Coumaric Acids / Q000378:metabolism*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D055750:Phytophthora infestans / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000382:microbiology
chemical_list: D000577:Amides; D003373:Coumaric Acids