title: Association of DNA repair and xenobiotic pathway gene polymorphisms with genetic susceptibility to gastric cancer patients in West Bengal, India.
abstract: Gastric cancer is one of the most common malignancies in India. DNA repair gene or xenobiotic pathway gene polymorphisms have recently been shown to affect individual susceptibility to gastric cancer. Here, the possible interaction between common polymorphisms in X-ray repair cross complementing group I (XRCC1) gene and glutathione S-transferase (GST) genes (GSTM1, GSTT1 and GSTP1), smoking and alcohol consumption and overall survival in gastric cancer patients were evaluated. In this population-based case control study, 70 gastric cancer patients and 82 healthy controls were enrolled. The epidemiological data were collected by a standard questionnaire, and blood samples were collected from each individual. XRCC1 Arg194Trp, Arg280His and Arg399Gln polymorphisms were determined by polymerase chain reaction and direct DNA sequencing. GSTM1 and GSTT1 null polymorphisms and GSTP1 Ile105Val polymorphism were identified by multiplex polymerase chain reaction and restriction fragment length polymorphism (RFLP), respectively. The risk of gastric cancer was significantly elevated in individuals with XRCC1 Arg/Gln +Gln/Gln (p = 0.031; odds ratio = 2.32; 95 % confidence interval (CI) 1.07-5.06) and GSTP1 Val/Val genotype (p = 0.009; odds ratio = 8.64; 95 % CI 1.84-40.55). An elevated risk for GC was observed in smokers and alcohol consumers carrying GSTP1 Ile/Val +Val/Val genotype (p = 0.041; odds ratio = 3.71; 95 % CI 0.98-14.12; p = 0.002; odds ratio = 12.31; 95 % CI 1.71-88.59). These findings suggest that XRCC1 rs25487 and GSTP1 rs1695 can be considered as a risk factor associated with gastric cancer and might be used as a molecular marker for evaluating the susceptibility of the disease.
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Soumee
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Sudakshina
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Biswabandhu
authors.initials: B
authors.lastname: Bankura
authors.affiliation: Department of Surgery, Institute of Post Graduate Medical Education & Research, 244 A.J.C Bose Road, Kolkata, 700 020, West Bengal, India.
authors.forename: Makhan Lal
authors.initials: ML
authors.lastname: Saha
authors.affiliation: Department of Surgery, Institute of Post Graduate Medical Education & Research, 244 A.J.C Bose Road, Kolkata, 700 020, West Bengal, India.
authors.forename: Suvendu
authors.initials: S
authors.lastname: Maji
authors.affiliation: Department of Biotechnology, Mizoram University, Tanhril, Aizawl, Mizoram, P.O. Box No. 190, India.
authors.forename: Souvik
authors.initials: S
authors.lastname: Ghatak
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Arup Kumar
authors.initials: AK
authors.lastname: Pattanayak
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Susanta
authors.initials: S
authors.lastname: Sadhukhan
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Manalee
authors.initials: M
authors.lastname: Guha
authors.affiliation: Department of Biotechnology, Mizoram University, Tanhril, Aizawl, Mizoram, P.O. Box No. 190, India.
authors.forename: Senthil Kumar
authors.initials: SK
authors.lastname: Nachimuthu
authors.affiliation: Department of Oncogene Regulation and Viral Associated Human Cancer, Chittaranjan Cancer Research Institute, 37, S.P. Mukherjee Road, Kolkata, 700026, West Bengal, India.
authors.forename: Chinmay Kumar
authors.initials: CK
authors.lastname: Panda
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Biswanath
authors.initials: B
authors.lastname: Maity
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India. madhuzoo@yahoo.com.
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Das
mesh_terms: D016022:Case-Control Studies; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics*; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase
keywords: GSTP1; Gastric cancer; SNPs; XRCC1