title: RETRACTED: The Anaphase-Promoting Complex/Cyclosome Is Essential for Entry into Meiotic M-Phase.
abstract: Vertebrate immature oocytes are arrested at prophase of meiosis I (MI). Hormonal stimulation breaks this prophase-I arrest and induces re-entry into MI. The mechanism underlying meiotic resumption remains largely elusive. Here, we demonstrate that the anaphase-promoting complex/cyclosome (APC/C) in complex with Cdh1 has an unexpected function in meiosis in that it is essential for meiotic resumption. We identify the catalytic subunit of protein phosphatase 6 (PP6c) as the critical substrate whose APC/C(Cdh1)-mediated destruction is a prerequisite for the re-entry of immature Xenopus laevis oocytes into MI. Preventing PP6c destruction impairs activating autophosphorylation of Aurora A, a cell-cycle kinase critical for meiotic translation. Restoring meiotic translation rescues the meiotic resumption defect of Cdh1-depleted oocytes. Thus, our studies discover that the essential function of the APC/C in triggering cell-cycle transitions is not limited to M-phase exit but also applies to entry into meiotic M-phase, and identify a crucial APC/C-PP6c-Aurora A axis in the resumption of female meiosis.
authors.affiliation: Department of Molecular Genetics, Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Saurav
authors.initials: S
authors.lastname: Malhotra
authors.affiliation: Department of Biochemistry, OCISB, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Palakkad Krishnanunni
authors.initials: PK
authors.lastname: Vinod
authors.affiliation: Cell Cycle, Biotechnology Center, TU Dresden, 01307 Dresden, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Mansfeld
authors.affiliation: Department of Genetics, University of Bayreuth, Universitä tsstrasse 30, 95440 Bayreuth, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Stemmann
authors.affiliation: Department of Molecular Genetics, Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany. Electronic address: thomas.u.mayer@uni-konstanz.de.
authors.forename: Thomas U
authors.initials: TU
authors.lastname: Mayer