title: Comparative proteomic analysis of Litopenaeus vannamei gills after vaccination with two WSSV structural proteins.
abstract: White spot syndrome virus (WSSV) is one of the most devastating viral pathogens of cultured shrimp worldwide. Recently published papers show the ability of WSSV structural protein VP28 to vaccinate shrimp and raise protection against the virus. This study attempted to identify the joining proteins of the aforementioned shrimp quasi-immune response by proteomic analysis. The other envelope protein, VP36B, was used as the non-protective subunit vaccine control. Shrimp were intramuscularly injected with rVPs or PBS on day 1 and day 4 and then on day 7 their gill tissues were sampled. The two-dimensional electrophoresis (2-DE) patterns of gill proteins between vaccinated and PBS groups were compared and 20 differentially expressed proteins identified by mass spectrometry, some of which were validated in gill and hemocyte tissues using real-time quantitative RT-PCR. Many of identified proteins and their expression levels also linked with the shrimp response during WSSV infection. The list of up-regulated protein spots found exclusively in rVP28-vaccinated shrimp include calreticulin and heat shock protein 70 with chaperone properties, ubiquitin, and others. The two serine proteases, chymotrypsin and trypsin, were significantly increased in shrimp of both vaccinated groups compared to PBS controls. The information presented here should be useful for gaining insight into invertebrate immunity.
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, 81157, Taiwan, ROC.
authors.forename: Li-Hao
authors.initials: LH
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, 81157, Taiwan, ROC.
authors.forename: Shi-Wei
authors.initials: SW
authors.lastname: Lin
authors.affiliation: Tungkang Biotechnology Research Center, Fisheries Research Institute, Council of Agriculture, Pingtung, 92845, Taiwan, ROC.
authors.forename: Kuan-Fu
authors.initials: KF
authors.lastname: Liu
authors.affiliation: Aquaculture Division, Fisheries Research Institute, Council of Agriculture, Keelung, 20246, Taiwan, ROC.
authors.forename: Chin-I
authors.initials: CI
authors.lastname: Chang
authors.affiliation: Mariculture Research Center, Fisheries Research Institute, Council of Agriculture, Tainan, 72453, Taiwan, ROC.
authors.forename: Jinn-Rong
authors.initials: JR
authors.lastname: Hseu
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, 81157, Taiwan, ROC. Electronic address: jmtsai@webmail.nkmu.edu.tw.
authors.forename: Jyh-Ming
authors.initials: JM
authors.lastname: Tsai
mesh_terms: D000818:Animals; D005880:Gills / Q000276:immunology / Q000821:virology; D007113:Immunity, Innate; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D015678:Viral Structural Proteins; D014765:Viral Vaccines
keywords: Invertebrate immunity; Proteomic analysis; Shrimp; Vaccine; White spot syndrome virus (WSSV)