title: Genotyping of clinical and environmental Aspergillus flavus isolates from Iran using microsatellites.
abstract: Aspergillus flavus is the second most important Aspergillus species causing human infections in tropical countries. Despite an increasing number of infections of A. flavus in Iran, the molecular epidemiology of clinical and environmental strains has not been well studied. We used a panel of nine microsatellite markers to analyse the genetic relatedness of A. flavus. Microsatellite typing of 143 (n = 119 clinical and n = 24 environmental) isolates demonstrated 118 different genotypes. A possible outbreak at a pulmonary ward was discovered. The discriminatory power for the individual markers ranged from 0.4812 to 0.9457 and the panel of all nine markers combined yielded a diversity index of 0.9948. This high-resolution typing method assists in better understanding of the molecular epidemiology of A. flavus.
authors.affiliation: Department of Medical Mycology and Parasitology, Tehran University of Medical Science, Tehran, Iran.|Department of Medical Mycology and Parasitology, Kurdistan University of Medical Science, Sanandaj, Iran.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Khodavaisy
authors.affiliation: Department of Medical Mycology and Parasitology, Mazandaran University of Medical Science, Sari, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Badali
authors.affiliation: Department of Medical Mycology and Parasitology, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Sassan
authors.initials: S
authors.lastname: Rezaie
authors.affiliation: Department of Medical Mycology and Parasitology, Mazandaran University of Medical Science, Sari, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Nabili
authors.affiliation: Department of Pulmonary Diseases, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Keivan G
authors.initials: KG
authors.lastname: Moghadam
authors.affiliation: Department of Infectious Diseases, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shirin
authors.initials: S
authors.lastname: Afhami
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.
authors.forename: Ferry
authors.identifier: http://orcid.org/0000-0002-5622-1916
authors.initials: F
authors.lastname: Hagen
authors.affiliation: Department of Medical Mycology and Parasitology, Kurdistan University of Medical Science, Sanandaj, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Aala
authors.affiliation: Department of Medical Mycology and Parasitology, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Sayed-Jamal
authors.initials: SJ
authors.lastname: Hashemi
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.|Department of Medical Microbiology, Radboudumc, Nijmegen, The Netherlands.
authors.forename: Jacques F
authors.identifier: http://orcid.org/0000-0003-3253-6080
authors.initials: JF
authors.lastname: Meis
mesh_terms: D001228:Aspergillosis / Q000382:microbiology*; D001231:Aspergillus flavus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004783:Environmental Microbiology; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D007492:Iran; D018895:Microsatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods*
keywords: 
  Aspergillus flavus
  ; Iran; microsatellite typing