title: The correlation of virulence, pathogenicity, and itraconazole resistance with SAP activity in Candida albicans strains.
abstract: The relationship between SAP2 activity and drug resistance in Candida albicans was investigated by using itraconazole-resistant and itraconazole-sensitive C. albicans isolates. The precipitation zones were measured to analyze SAP2 activity. Mice were classified into itraconazole-resistant and -sensitive C. albicans isolate groups, and a control group, with their survival and mortality rate being observed over 30 days. The relative expression levels of CDR1, CDR2, MDR1, and SAP2 were measured using RT-PCR. It was found that the secreted aspartyl proteinase activity of itraconazole-resistant C. albicans strains was significantly higher than that of itraconazole-sensitive C. albicans strains (P < 0.001). A significantly higher mortality rate was recorded for mice treated with itraconazole-resistant C. albicans than for mice treated with itraconazole-sensitive C. albicans. In regards to the CDR1, CDR2, and MDR1 genes, there was no significant difference between the 2 groups of mice. Positive correlations between SAP2 and MDR1 and between CDR1 and CDR2 were found. The high expression level of SAP2 may relate to the virulence, pathogenicity, and resistance of C. albicans.
authors.affiliation: Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.|Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Feng
authors.affiliation: Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.|Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.|Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.|Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xi
authors.affiliation: Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.|Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.
authors.forename: Zusha
authors.initials: Z
authors.lastname: Qiao
authors.affiliation: Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.|Department of Dermatovenereology, The Second Hospital of ShanXi Medical University, No. 382 WuYi Road, Taiyuan City 030001, ShanXi Province, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology / Q000472:pathogenicity*; D002177:Candidiasis / Q000401:mortality; D025141:Drug Resistance, Fungal; D005260:Female; D005656:Fungal Proteins / Q000378:metabolism*; D017964:Itraconazole / Q000494:pharmacology*; D008297:Male; D051379:Mice; D014774:Virulence
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D017964:Itraconazole; D016282:Aspartic Acid Endopeptidases; C090753:SAP2 protein, Candida
keywords: Candida albicans; pathogenicity; pathogénicité; protéinase aspartique sécrétée (SAP); resistance; résistance; secreted aspartyl proteinase (SAP); virulence