title: 2-Amino-2-deoxy-glucose conjugated cobalt ferrite magnetic nanoparticle (2DG-MNP) as a targeting agent for breast cancer cells.
abstract: In this study, 2-amino-2-deoxy-glucose (2DG) was conjugated to COOH modified cobalt ferrite magnetic nanoparticles (COOH-MNPs), which were designed to target tumor cells as a potential targetable drug/gene delivery agent for cancer treatment. According to our results, it is apparent that, 2DG labeled MNPs were internalized more efficiently than COOH-MNPs under the same conditions in all cell types (MDA-MB-231 and MCF-7 cancer and MCF-10A normal breast cells) (p<0.001). Moreover, the highest amount of uptake was observed in MDA-MB-231, followed by MCF-7 and normal MCF-10A cells for both MNPs. The apoptotic effects of 2DG-MNPs were further evaluated, and it was found that apoptosis was not induced at low concentrations of 2DG-MNPs in all cell types, whereas dramatic cell death was observed at higher concentrations. In addition, the gene expression levels of four drug-metabolizing enzymes, two Phase I (CYP1A1, CYP1B1) and two Phase II (GSTM3, GSTZ1) were also increased with the high concentrations of 2DG-MNPs.
authors.affiliation: Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Aşık
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey.
authors.forename: Tuğba Nur
authors.initials: TN
authors.lastname: Aslan
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey.
authors.forename: Mürvet
authors.initials: M
authors.lastname: Volkan
authors.affiliation: Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Biological Sciences, Middle East Technical University, Ankara 06800, Turkey. Electronic address: guray@metu.edu.tr.
authors.forename: N Tülin
authors.initials: NT
authors.lastname: Güray
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry* / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005944:Glucosamine / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C569492:cobalt ferrite; D003035:Cobalt; D005944:Glucosamine
keywords: 2-Amino-2-deoxy-glucose; Breast cancer; Cobalt ferrite nanoparticles; Targeting therapy