title: Purification and Partial Characterization of a Novel Bacteriocin Synthesized by Lactobacillus paracasei HD1-7 Isolated from Chinese Sauerkraut Juice.
abstract: Bacteriocins have antimicrobial activities against food-spoiling bacteria and food-borne pathogens. Paracin 1.7, a bacteriocin synthesized by Lactobacillus paracasei HD1-7 isolated from Chinese sauerkraut juice, was studied. Following partial purification with ammonium sulfate precipitation, CM Sepharose Fast Flow, and Sephadex G-10 chromatography, the molecular weight of Paracin 1.7 was about 10 kDa based on Tricine-SDS-PAGE results. A 2.87 fold purified bacteriocin was produced, reaching a final yield of 39.93% and the specific activity of 1.56 × 10(3) AU/mg. The N-terminal amino acid sequence of Paracin 1.7 was VSNTFFA, and the LC/LTQ results revealed that the N-terminal amino acid sequence was similar to that of ABC-type oligopeptide transport system protein and N-acetylmuramoyl-L-alanine amidase. Paracin 1.7 was sensitive to protease K, had antimicrobial activities at a broad pH range (3.0-8.0), and was heat resistant (121 °C for 20 min). Paracin 1.7 from Lactobacillus paracasei HD1-7 is a novel bacteriocin that has potential applications in food preservation.
authors.affiliation: Heilongjiang University, Harbin, Heilongjiang, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Ge
authors.affiliation: Heilongjiang University, Harbin, Heilongjiang, China.
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Heilongjiang University, Harbin, Heilongjiang, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Xin
authors.affiliation: Heilongjiang University, Harbin, Heilongjiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Heilongjiang University, Harbin, Heilongjiang, China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Ping
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D000069980:Lacticaseibacillus paracasei / Q000378:metabolism*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins