title: Bilaminar Device of Poly(Lactic-co-Glycolic Acid)/Collagen Cultured With Adipose-Derived Stem Cells for Dermal Regeneration.
abstract: Several materials are commercially available as substitutes for skin. However, new strategies are needed to improve the treatment of skin wounds. In this study, we developed and characterized a new device consisting of poly(lactic-co-glycolic acid) (PLGA) and collagen associated with mesenchymal stem cells derived from human adipose tissue. To develop the bilaminar device, we initially obtained a membrane of PLGA by dissolving the copolymer in chloroform and then produced a collagen type I scaffold by freeze-drying. The materials were characterized physically by gel permeation chromatography, scanning electron microscopy, and mass loss. Biological activity was assessed by cell proliferation assay. A preliminary study in vivo was performed with a pig model in which tissue regeneration was assessed macroscopically and histologically, the commercial device Integra being used as a control. The PLGA/collagen bilaminar material was porous, hydrolytically degradable, and compatible with skin growth. The polymer complex allowed cell adhesion and proliferation, making it a potentially useful cell carrier. In addition, the transparency of the material allowed monitoring of the lesion when the dressings were changed. Xenogeneic mesenchymal cells cultured on the device (PLGA/collagen/ASC) showed a reduced granulomatous reaction to bovine collagen, down-regulation of α-SMA, enhancement in the number of neoformed blood vessels, and collagen organization as compared with normal skin; the device was superior to other materials tested (PLGA/collagen and Integra) in its ability to stimulate the formation of new cutaneous tissue.
authors.affiliation: Department of Cell Biology and Structural, Biology Institute, UNICAMP, Campinas, Brazil.
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Domingues
authors.affiliation: Department of Materials Engineering, Faculty of Mechanical Engineering, UNICAMP, Campinas, Brazil.
authors.forename: Giselle
authors.initials: G
authors.lastname: Cherutti
authors.affiliation: Department of Physiological Sciences, Biomaterials Laboratory, PUC- SP, Sorocaba, Brazil.
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Motta
authors.affiliation: Department of Biology, Structural Biology Laboratory, UFSCAR, Sorocaba, Brazil.
authors.forename: Moema A
authors.initials: MA
authors.lastname: Hausen
authors.affiliation: Department of Physiological Sciences, Biomaterials Laboratory, PUC- SP, Sorocaba, Brazil.
authors.forename: Rômulo T D
authors.initials: RT
authors.lastname: Oliveira
authors.affiliation: Department of Biology, Structural Biology Laboratory, UFSCAR, Sorocaba, Brazil.
authors.forename: Elaine C M
authors.initials: EC
authors.lastname: Silva-Zacarin
authors.affiliation: Department of Physiological Sciences, Biomaterials Laboratory, PUC- SP, Sorocaba, Brazil.
authors.forename: Maria Lourdes P
authors.initials: ML
authors.lastname: Barbo
authors.affiliation: Department of Materials Engineering, Faculty of Mechanical Engineering, UNICAMP, Campinas, Brazil. eliduek@pucsp.br.|Department of Physiological Sciences, Biomaterials Laboratory, PUC- SP, Sorocaba, Brazil. eliduek@pucsp.br.
authors.forename: Eliana A R
authors.initials: EA
authors.lastname: Duek
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012038:Regeneration; D012867:Skin / Q000166:cytology / Q000293:injuries / Q000648:ultrastructure; D012879:Skin Physiological Phenomena; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen
keywords: Collagen scaffold; Dermal; Mesenchymal stem cells; Poly(lactic-co-glycolic acid); Tissue regeneration