title: Chemopreventive Effects of Eryngium foetidum L. Leaves on COX-2 Reduction in Mice Induced Colorectal Carcinogenesis.
abstract: To investigate the potential effects of Eryngium foetidum Linn. leaves (EF) in colitis-induced colorectal carcinogenesis in mice by azoxymethane (AOM) and dextran sulfate sodium (DSS), 39 ICR male mice were studied and divided into 6 groups. The mice were received a modified AIN-76 diet in Group 1, whereas Group 2 was given an AOM, DSS, and AIN-76 diet. Groups 3 and 4 were fed with 0.8% and 3.2% freeze-dried EF with AIN-76 diets, for 5 wk. Groups 5 and 6 were fed with 0.8% and 3.2% EF diets for 5 wk during AOM/DSS administration. The mice were necropsied at Week 20 and their colons were collected. The results indicated that the incidences of tumors in Groups 2, 5, and 6 was 100%, 75%, and 88%, with multiplicities (mean ±SE) of 3.75 ±0.92, 2.38 ± 0.96 and 4.25 ± 0.79, respectively. Interestingly, there was a significant difference in COX-2 expression in mice received 3.2% EF in their diet, but the proliferative cell nuclear antigen index and iNOS protein expression were not significantly different. We concluded that EF at a dose level of 3.2% in their diet had a preventive effect on colorectal carcinogenesis via the proinflammatory cytokine, COX-2.
authors.affiliation: a Institute of Nutrition, Mahidol University , Nakhon Pathom , Thailand.
authors.forename: Kamonwan
authors.initials: K
authors.lastname: Promtes
authors.affiliation: b Laboratory Animal Section, National Cancer Institute , Bangkok , Thailand.
authors.forename: Piengchai
authors.initials: P
authors.lastname: Kupradinun
authors.affiliation: c Companion Animal Cancer Research Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University , Bangkok , Thailand.
authors.forename: Anudep
authors.initials: A
authors.lastname: Rungsipipat
authors.affiliation: a Institute of Nutrition, Mahidol University , Nakhon Pathom , Thailand.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Tuntipopipat
authors.affiliation: a Institute of Nutrition, Mahidol University , Nakhon Pathom , Thailand.
authors.forename: Chaniphun
authors.initials: C
authors.lastname: Butryee
mesh_terms: D000818:Animals; D001835:Body Weight; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D031049:Eryngium; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D018515:Plant Leaves; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2