title: Investigation of single nucleotide polymorphisms based on the intronic sequences of the propylene alcohol dehydrogenase gene in Chinese tobacco genotypes.
abstract: A pair of primers was designed to amplify the propylene alcohol dehydrogenase gene sequence based on the cDNA sequence of the tobacco allyl-alcohol dehydrogenase gene. All introns were sequenced using traditional polymerase chain reaction (PCR) methods and T-A cloning. The sequences from common tobacco (Nicotiana tabaccumL.) and rustica tobacco (Nicotiana rusticaL.) were analysed between the third intron and the fourth intron of the propylene alcohol dehydrogenase gene. The results showed that the alcohol dehydrogenase gene is a low-copy nuclear gene. The intron sequences have a combination of single nucleotide polymorphisms and length polymorphisms between common tobacco and rustica tobacco, which are suitable to identify the different germplasms. Furthermore, there are some single nucleotide polymorphism sites in the target sequence within common tobacco that can be used to distinguish intraspecific varieties.
authors.affiliation: Department of Biology, Mudanjiang Teachers College , Mudanjiang , P.R. China.
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Wei
authors.affiliation: Laboratory of Breeding, Mudanjiang Tobacco Research Institute , Mudanjiang , P.R. China.
authors.forename: En-Jian
authors.initials: EJ
authors.lastname: Qiu
authors.affiliation: Department of Biology, Mudanjiang Teachers College , Mudanjiang , P.R. China.
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Guo
authors.affiliation: Department of Biology, Mudanjiang Teachers College , Mudanjiang , P.R. China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Hao
authors.affiliation: Laboratory of Breeding, Mudanjiang Tobacco Research Institute , Mudanjiang , P.R. China.
authors.forename: Rong-Ping
authors.initials: RP
authors.lastname: Chen
keywords: intron polymorphisms; propylene alcohol dehydrogenase gene; tobacco