title: Effects of PVA-coated nanoparticles on human T helper cell activity.
abstract: Superparamagnetic iron oxide nanoparticles (SPION) are used as high-sensitive enhancer for magnetic resonance imaging, where they represent a promising tool for early diagnosis of destructive diseases such as rheumatoid arthritis (RA). Since we could demonstrate that professional phagocytes are activated by amino-polyvinyl-alcohol-coated-SPION (a-PVA-SPION), the study here focuses on the influence of a-PVA-SPION on human T cells activity. Therefore, primary human CD4+ T cells from RA patients and healthy subjects were treated with varying doses of a-PVA-SPION for 20h or 72h. T cells were then analyzed for apoptosis, cellular energy, expression of the activation marker CD25 and cell proliferation. Although, we observed that T cells from RA patients are more susceptible to low-dose a-PVA-SPION-induced apoptosis than T cells from healthy subjects, in both groups a-PVA-SPION do not activate CD4+ T cells per se and do not influence mitogen-mediated T cells activation with regard to CD25 expression and cell proliferation. Nevertheless, our results demonstrate that CD4+ T cells from RA patients and healthy subjects differ in their response to mitogen stimulation and oxygen availability. We conclude from our data, that a-PVA-SPION do neither activate nor significantly influence mitogen-stimulated CD4+ T cells activation and have negligible influence on T cells apoptosis.
authors.affiliation: Charité University Hospital, Department of Rheumatology and Clinical Immunology, 10117 Berlin, Germany; German Rheumatism Research Centre (DRFZ), 10117 Berlin, Germany. Electronic address: cindy.strehl@charite.de.
authors.forename: Cindy
authors.initials: C
authors.lastname: Strehl
authors.affiliation: Charité University Hospital, Department of Rheumatology and Clinical Immunology, 10117 Berlin, Germany; German Rheumatism Research Centre (DRFZ), 10117 Berlin, Germany.
authors.forename: Saskia
authors.initials: S
authors.lastname: Schellmann
authors.affiliation: Powder Technology Laboratory, Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Lionel
authors.initials: L
authors.lastname: Maurizi
authors.affiliation: MatSearch Consulting Hofmann, CH-1009 Pully-Lausanne, Switzerland.
authors.forename: Margarethe
authors.initials: M
authors.lastname: Hofmann-Amtenbrink
authors.affiliation: Charité University Hospital, Department of Rheumatology and Clinical Immunology, 10117 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Häupl
authors.affiliation: Powder Technology Laboratory, Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofmann
authors.affiliation: Charité University Hospital, Department of Rheumatology and Clinical Immunology, 10117 Berlin, Germany; German Rheumatism Research Centre (DRFZ), 10117 Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Buttgereit
authors.affiliation: Charité University Hospital, Department of Rheumatology and Clinical Immunology, 10117 Berlin, Germany; German Rheumatism Research Centre (DRFZ), 10117 Berlin, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Gaber
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001172:Arthritis, Rheumatoid / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D005290:Ferric Compounds / Q000633:toxicity; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008280:Magnetics; D008934:Mitogens / Q000494:pharmacology; D053758:Nanoparticles / Q000633:toxicity*; D010101:Oxygen Consumption / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000633:toxicity*; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects*
chemical_list: D005290:Ferric Compounds; D053645:Interleukin-2 Receptor alpha Subunit; D008934:Mitogens; C000499:ferric oxide; D011142:Polyvinyl Alcohol
keywords: Apoptosis; Cellular energy metabolism; Human T helper cells; Iron oxide nanoparticles; Proliferation