title: MiR-381 inhibits epithelial ovarian cancer malignancy via YY1 suppression.
abstract: Epithelial ovarian cancer (EOC) is a common type of gynecologic cancer, which accounts for the majority of deaths among all gynecologic malignant tumors in developed countries. A series of recent studies suggested that miR-381 might play important roles in the development of various cancer types. However, the biological function of miR-381 in EOC remains to be investigated. We examined the levels of miR-381 expression in EOC tissues and cell lines. We identified miR-381 target genes by bioinformatic prediction. We also characterized the phenotype regarding cell proliferation, cell migration, and cell invasion in EOC cells lines with altered expression levels of both miR-381 and its target gene, YY1. The expression levels of miR-381 were downregulated in EOC tissues and cell lines. Overexpression of miR-381 significantly inhibited EOC cell proliferation, migration, and invasion. Restoration of YY1 expression partially reversed the phenotype induced by miR-381 overexpression. Knockdown of miR-381 target gene, YY1, mimicked the phenotype induced by miR-381 overexpression. MiR-381 regulated EOC cell through miR-381/YY1/p53 and miR-381/YY1/Wnt signaling axis. We concluded that miR-381 inhibited EOC cell proliferation, migration, and invasion, at least in part, via suppressing YY1 expression.
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Nangang, Harbin, Heilongjiang, 150020, China.
authors.forename: Bairong
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Nursing, the Affiliated Tumor Hospital, Harbin Medical University, Harbin, 150020, China.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Nangang, Harbin, Heilongjiang, 150020, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Nangang, Harbin, Heilongjiang, 150020, China.
authors.forename: Tianbo
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Nangang, Harbin, Heilongjiang, 150020, China. lougehyd@163.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Lou
mesh_terms: D000077216:Carcinoma, Ovarian Epithelial; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D050986:YY1 Transcription Factor / Q000235:genetics*
chemical_list: C588249:MIRN381 microRNA, human; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; D050986:YY1 Transcription Factor; C494698:YY1 protein, human
keywords: Cell invasion; Cell migration; Cell proliferation; Epithelial ovarian cancer; YY1; miR-381