title: Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology.
abstract: In present study, the beneficial effect of corncob acid hydrolysate environment on lipid fermentation of Trichosporon dermatis was elucidated by method of cell biology (mainly using flow cytometry and microscope) for the first time. Propidium iodide (PI) and rhodamine 123 (Rh123) staining showed that corncob acid hydrolysate environment was favorable for the cell membrane integrity and mitochondrial membrane potential of T. dermatis and thus made its lipid fermentation more efficient. Nile red (NR) staining showed that corncob acid hydrolysate environment made the lipid accumulation of T. dermatis slower, but this influence was not serious. Moreover, the cell morphology of T. dermatis elongated in the corncob acid hydrolysate, but the cell morphology changed as elliptical-like during fermentation. Overall, this work offers one simple and effective method to evaluate the influence of lignocellulosic hydrolysates environment on lipid fermentation.
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Xue-Fang
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Lin
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Gao-Xiang
authors.initials: GX
authors.lastname: Qi
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Si-Lan
authors.initials: SL
authors.lastname: Shi
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China. cxd_cxd@hotmail.com.|Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China. cxd_cxd@hotmail.com.|, No. 2 Nengyuan Road, Tianhe District, Guangzhou, 510640, People's Republic of China. cxd_cxd@hotmail.com.
authors.forename: Xin-De
authors.initials: XD
authors.lastname: Chen
mesh_terms: D000143:Acids / Q000737:chemistry; D001487:Basidiomycota / Q000737:chemistry; D002462:Cell Membrane / Q000187:drug effects; D060833:Cellular Microenvironment / Q000235:genetics; D003872:Dermatitis / Q000378:metabolism* / Q000382:microbiology; D005285:Fermentation; D005434:Flow Cytometry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry*; D014250:Trichosporon / Q000737:chemistry / Q000201:enzymology; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
chemical_list: D000143:Acids; D008055:Lipids; D011492:Protein Hydrolysates; C036909:lignocellulose; D008031:Lignin
keywords: Cell biology; Corncob acid hydrolysate; Flow cytometry; Microbial oil; Trichosporon dermatis