title: Silvibacterium bohemicum gen. nov. sp. nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park.
abstract: During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs. Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length. Its pH range for growth was 3-6, with optimal conditions found at approximately 4-5. It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%). The G+C DNA content was 58.2mol%. According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T). Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin. Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp. nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
authors.affiliation: Institute of Microbiology ASCR, Laboratory of Environmental Microbiology, Prague, Czech Republic.
authors.forename: Salvador
authors.initials: S
authors.lastname: Lladó
authors.affiliation: Institute of Microbiology ASCR, Laboratory of Molecular Structure Characterization, Prague, Czech Republic.
authors.forename: Oldrich
authors.initials: O
authors.lastname: Benada
authors.affiliation: Institute of Microbiology ASCR, Laboratory of Environmental Biotechnology, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Cajthaml
authors.affiliation: Institute of Microbiology ASCR, Laboratory of Environmental Microbiology, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Baldrian
authors.affiliation: Institute of Microbiology ASCR, Laboratory of Fungal Genetics and Metabolism, Prague, Czech Republic. Electronic address: garcia@biomed.cas.cz.
authors.forename: Paula
authors.initials: P
authors.lastname: García-Fraile
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D050260:Carbohydrate Metabolism / Q000235:genetics; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D065928:Forests; D016680:Genome, Bacterial / Q000235:genetics; D000068316:Parks, Recreational; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D064028:Tracheophyta
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S
keywords: Acidobacteria; Bohemian Forest National Park; Coniferous soil; Taxonomy