title: Study of Mast Cells and Granules from Primo Nodes Using Scanning Ionic Conductance Microscopy.
abstract: Acupuncture points have a notable characteristic in that they have a higher density of mast cells (MCs) compared with nonacupoints in the skin, which is consistent with the augmentation of the immune function by acupuncture treatment. The primo vascular system, which was proposed as the anatomical structure of the acupuncture points and meridians, also has a high density of MCs. We isolated the primo nodes from the surfaces of internal abdominal organs, and the harvested primo nodes were stained with toluidine blue. The MCs were easily recognized by their stained color and their characteristic granules. The MCs were classified into four stages according to the degranulation of histamine granules in the MCs. Using conventional optical microscopes details of the degranulation state of MCs in each stage were not observable. However, we were able to investigate the distribution of the granules on the surfaces of the MCs in each stage, and to demonstrate the height profiles and three-dimensional structures of the MCs without disturbance of the cell membrane by using the scanning ion conductance microscopy.
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, South Korea.
authors.forename: Yeong-Yung
authors.initials: YY
authors.lastname: Yoo
authors.affiliation: Research & Development Center, Park System Inc., Suwon, South Korea; Department of Physics, Korea University, Seoul, South Korea.
authors.forename: Goo-Eun
authors.initials: GE
authors.lastname: Jung
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul, South Korea.
authors.forename: Hee-Min
authors.initials: HM
authors.lastname: Kwon
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, South Korea. Electronic address: wushu117@gmail.com.
authors.forename: Kyoung-Hee
authors.initials: KH
authors.lastname: Bae
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, South Korea; Research & Development Center, Park System Inc., Suwon, South Korea. Electronic address: msjchok@gmail.com.
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Cho
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, South Korea.
authors.forename: Kwang-Sup
authors.initials: KS
authors.lastname: Soh
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D008297:Male; D008407:Mast Cells / Q000737:chemistry* / Q000166:cytology; D016740:Meridians; D008853:Microscopy; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: acupuncture; histamine granule; mast cell; primo vascular system; scanning ion conductance microscope (SICM)