title: Molecular and Functional Characterization of a Polygalacturonase-Inhibiting Protein from Cynanchum komarovii That Confers Fungal Resistance in Arabidopsis.
abstract: Compliance with ethical standards: This study did not involve human participants and animals, and the plant of interest is not an endangered species. Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat proteins that plants produce against polygalacturonase, a key virulence agent in pathogens. In this paper, we cloned and purified CkPGIP1, a gene product from Cynanchum komarovii that effectively inhibits polygalacturonases from Botrytis cinerea and Rhizoctonia solani. We found the expression of CkPGIP1 to be induced in response to salicylic acid, wounding, and infection with B. cinerea and R. solani. In addition, transgenic overexpression in Arabidopsis enhanced resistance against B. cinerea. Furthermore, CkPGIP1 obtained from transgenic Arabidopsis inhibited the activity of B. cinerea and R. solani polygalacturonases by 62.7-66.4% and 56.5-60.2%, respectively. Docking studies indicated that the protein interacts strongly with the B1-sheet at the N-terminus of the B. cinerea polygalacturonase, and with the C-terminus of the polygalacturonase from R. solani. This study highlights the significance of CkPGIP1 in plant disease resistance, and its possible application to manage fungal pathogens.
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Liu
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Sihong
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Xiancai
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000382:microbiology*; D020171:Botrytis / Q000201:enzymology; D003001:Cloning, Molecular; D031105:Cynanchum / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance / Q000235:genetics; D005786:Gene Expression Regulation; D020128:Inhibitory Concentration 50; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011096:Polygalacturonase / Q000037:antagonists & inhibitors; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012232:Rhizoctonia / Q000201:enzymology; D020156:Salicylic Acid / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: C081479:PGIP protein, plant; D010936:Plant Extracts; D010940:Plant Proteins; D011096:Polygalacturonase; D020156:Salicylic Acid