title: Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat.
abstract: OBJECTIVE
  We aimed to evaluate whether pancreatic kininogenase (PKase) can relieve renal fibrosis and investigate its mechanisms in diabetic nephropathy (DN) rats Methods: We established streptozotocin (STZ) induced-DN rats. After treatment with PKase for 4 weeks, urinary weight, urinary protein content and blood glucose concentration were detected, and then renal histopathological changes were examined using Hematoxylin and Eosin (H&amp;E) and Masson's thrchrome staining. In addition, the expressions of miR-433, transforming growth factor-β1 (TGF-β1) and antizyme inhibitor 1 (Azin1) were detected by qRT-PCR and/or western blotting.
  
  
  RESULTS
  PKase reduced urinary weight, urinary protein contents and blood glucose concentrations. PKase treated DN rats exhibited less renal fibrosis than untreated DN rats (P < 0.05). Furthermore, the expression levels of TGF-β and miR-433 were reduced (P < 0.05), while Azin1 expression was increased in renal tissues of PKase treated DN rats compared with untreated DN rats (P < 0.05).
  
  
  CONCLUSIONS
  PKase might not only inhibit the development of DN by reducing urinary weight, urinary protein content and blood glucose concentration in DN rats, but also relieve renal fibrosis in DN rats through inhibiting the expression of TGF-β1, and miR-433 and Azin1 might involve in this process.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Heilongjiang, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Cheng
authors.forename: Liansheng
authors.initials: L
authors.lastname: Ren
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Dianjun
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D007610:Kallikreins / Q000494:pharmacology / Q000627:therapeutic use*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010179:Pancreas; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D013311:Streptozocin; D007610:Kallikreins