title: Mass spectrometric detection of the amino acid sequence polymorphism of the hepatitis C virus antigen.
abstract: A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force microscope (AFM chip) with subsequent protein identification by tandem mass spectrometric (MS/MS) analysis. Biospecific AFM-capturing of viral particles containing HCVcoreAg from serum samples was performed by use of AFM chips with monoclonal antibodies (anti-HCVcore) covalently immobilized on the surface. Biospecific complexes were registered and counted by AFM. Further MS/MS analysis allowed to reliably identify the HCVcoreAg in the complexes formed on the AFM chip surface. Analysis of MS/MS spectra, with the account taken of the possible polymorphisms in the amino acid sequence of the HCVcoreAg, enabled us to increase the number of identified peptides.
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia. Electronic address: kaysheva@gmail.com.
authors.forename: A L
authors.initials: AL
authors.lastname: Kaysheva
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: Yu D
authors.initials: YD
authors.lastname: Ivanov
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Frantsuzov
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Krohin
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Pavlova
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: V F
authors.initials: VF
authors.lastname: Uchaikin
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: V А
authors.initials: VА
authors.lastname: Konev
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: O B
authors.initials: OB
authors.lastname: Kovalev
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Ziborov
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow 119121 Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D019943:Amino Acid Substitution; D002846:Chromatography, Affinity; D018936:Hepatitis C Antigens / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D011485:Protein Binding; D044967:Serum / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014758:Viral Core Proteins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D018936:Hepatitis C Antigens; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus
keywords: HCVcoreAg detection; Hepatitis C; Mass spectrometry; SAP