title: Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis.
abstract: BACKGROUND
  Human gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human gnathostomiasis.
  
  
  RESULTS
  The recombinant protein (named rGslic18) was applied to ICT kit as the antigen. The diagnostic value of ICT kit was evaluated using serum samples from parasitologically proven and clinically suspected gnathostomiasis patients, healthy volunteers and patients with other parasitic diseases. The ICT kit exhibited quite high sensitivity (93.75%) and specificity (97.01%).
  
  
  CONCLUSIONS
  The ICT kit is simple, convenient and easy to implement and expected to provide reliable diagnostic results for human gnathostomiasis. It also will be a promising diagnostic tool not only for large-scale epidemiological surveys in endemic or remote areas where diagnostic facilities are poor but also for a rapid clinical diagnosis in the bedside laboratory.
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. pair.wu@gmail.com.|Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, 80161, Thailand. pair.wu@gmail.com.
authors.forename: Penchom
authors.initials: P
authors.lastname: Janwan
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. pewpan@kku.ac.th.|Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. pewpan@kku.ac.th.
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan. hyamasak@niid.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. rutchanee5020@gmail.com.|Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. rutchanee5020@gmail.com.
authors.forename: Rutchanee
authors.initials: R
authors.lastname: Rodpai
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. porlau@kku.ac.th.|Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. porlau@kku.ac.th.
authors.forename: Porntip
authors.initials: P
authors.lastname: Laummaunwai
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. thanchomnang@yahoo.com.|Faculty of Medicine, Mahasarakham University, Mahasarakham, 44000, Thailand. thanchomnang@yahoo.com.
authors.forename: Tongjit
authors.initials: T
authors.lastname: Thanchomnang
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. sanpoolor@yahoo.com.|Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. sanpoolor@yahoo.com.|Faculty of Medicine, Mahasarakham University, Mahasarakham, 44000, Thailand. sanpoolor@yahoo.com.
authors.forename: Oranuch
authors.initials: O
authors.lastname: Sanpool
authors.affiliation: Division of Research and Development, Adtec Inc., Oita, 879-0471, Japan. k-kobayashi@adtec-inc.co.jp.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Division of Research and Development, Adtec Inc., Oita, 879-0471, Japan. k-takayama@adtec-inc.co.jp.
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Takayama
authors.affiliation: Division of Research and Development, Adtec Inc., Oita, 879-0471, Japan. y-kobayashi@adtec-inc.co.jp.
authors.forename: Yukuharu
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. wanch_ma@kku.ac.th.|Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. wanch_ma@kku.ac.th.
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D002846:Chromatography, Affinity / Q000379:methods*; D058429:Gnathostomiasis / Q000175:diagnosis*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013997:Time Factors
chemical_list: D000906:Antibodies; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins