title: Protein-targeted corona phase molecular recognition.
abstract: Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Gili
authors.identifier: http://orcid.org/0000-0003-2592-7956
authors.initials: G
authors.lastname: Bisker
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Juyao
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Hoyoung D
authors.initials: HD
authors.lastname: Park
authors.affiliation: Department of Biological Systems Engineering, University of Nebraska-Lincoln, 223 L.W. Chase Hall, Lincoln, Nebraska 68583, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Iverson
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Justin T
authors.initials: JT
authors.lastname: Nelson
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley, 201 Gilman Hall, Berkeley, California 94720, USA.
authors.forename: Markita P
authors.initials: MP
authors.lastname: Landry
authors.affiliation: Department of Chemistry, Institute for Physical Chemistry, Göttingen University, Tammannstrasse 6, 37077 Göttingen, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kruss
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Strano
mesh_terms: D001798:Blood Proteins / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry*; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D037742:Nanotubes, Carbon; D011108:Polymers; D000066970:Protein Corona
chemical_list: D001798:Blood Proteins; D037742:Nanotubes, Carbon; D011108:Polymers; D000066970:Protein Corona; D005340:Fibrinogen