title: Human S100A10 plays a crucial role in the acquisition of the endometrial receptivity phenotype.
abstract: In assisted reproduction, about 30% of embryo implantation failures are related to inadequate endometrial receptivity. To identify molecules involved in endometrial receptivity acquisition, we investigated, using a SELDI-TOF approach, the protein expression profile of early-secretory and mid-secretory endometrium samples. Among the proteins upregulated in mid-secretory endometrium, we investigated the function of S100A10 in endometrial receptivity and implantation process. S100A10 was expressed in epithelial and stromal cells of the endometrium of fertile patients during the implantation windows. Conversely, it was downregulated in the mid-secretory endometrium of infertile patients diagnosed as non-receptive. Transcriptome analysis of human endometrial epithelial and stromal cells where S100A10 was silenced by shRNA revealed the deregulation of 37 and 256 genes, respectively, related to components of the extracellular matrix and intercellular connections. Functional annotations of these deregulated genes highlighted alterations of the leukocyte extravasation signaling and angiogenesis pathways that play a crucial role during implantation. S100A10 silencing also affected the migration of primary endometrial epithelial and stromal cells, decidualization and secretory transformation of primary endometrial stromal cells and epithelial cells respectively, and promoted apoptosis in serum-starved endometrial epithelial cells. Our findings identify S100A10 as a player in endometrial receptivity acquisition.
authors.affiliation: a Inserm U1203, 'Développement embryonnaire précoce humain et pluripotence', Hôpital Saint-Eloi , Montpellier , France.|b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.|c Université de Montpellier, UFR de Médecine , Montpellier , France.|d OVO Fertility , Montréal , Québec , Canada.
authors.forename: Laurence
authors.initials: L
authors.lastname: Bissonnette
authors.affiliation: a Inserm U1203, 'Développement embryonnaire précoce humain et pluripotence', Hôpital Saint-Eloi , Montpellier , France.|b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.|c Université de Montpellier, UFR de Médecine , Montpellier , France.
authors.forename: Loubna
authors.initials: L
authors.lastname: Drissennek
authors.affiliation: a Inserm U1203, 'Développement embryonnaire précoce humain et pluripotence', Hôpital Saint-Eloi , Montpellier , France.|b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Antoine
authors.affiliation: b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Tiers
authors.affiliation: b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.|c Université de Montpellier, UFR de Médecine , Montpellier , France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
authors.affiliation: b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.|c Université de Montpellier, UFR de Médecine , Montpellier , France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
authors.affiliation: e CHU Montpellier, Hôpital Gui de Chauliac, Service Anatomie cytologie pathologiques , Montpellier , France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Perrochia
authors.affiliation: d OVO Fertility , Montréal , Québec , Canada.
authors.forename: François
authors.initials: F
authors.lastname: Bissonnette
authors.affiliation: d OVO Fertility , Montréal , Québec , Canada.
authors.forename: Isaac-Jacques
authors.initials: IJ
authors.lastname: Kadoch
authors.affiliation: a Inserm U1203, 'Développement embryonnaire précoce humain et pluripotence', Hôpital Saint-Eloi , Montpellier , France.|b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.|c Université de Montpellier, UFR de Médecine , Montpellier , France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Haouzi
authors.affiliation: a Inserm U1203, 'Développement embryonnaire précoce humain et pluripotence', Hôpital Saint-Eloi , Montpellier , France.|b CHU Montpellier, Institut de Médecine Régénératrice et de Biothérapie, Hôpital Saint-Eloi , Montpellier , France.|c Université de Montpellier, UFR de Médecine , Montpellier , France.|f CHU Montpellier, Département de Biologie de la Reproduction et du DPI, Hôpital Arnaud de Villeneuve , Montpellier , France.
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D000328:Adult; D017306:Annexin A2 / Q000378:metabolism*; D017209:Apoptosis; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D015536:Down-Regulation; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D013058:Mass Spectrometry; D008597:Menstrual Cycle; D010641:Phenotype; D011247:Pregnancy; D040901:Proteomics; D009418:S100 Proteins / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics; D014327:Trophoblasts / Q000166:cytology; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C072337:S100A11 protein, human
keywords: S100A10; early implantation; endometrial receptivity; human; proteome