title: Evaluation of local trace element status and 8-Iso-prostaglandin F2α concentrations in patients with Tinea pedis.
abstract: BACKGROUND
  Tinea pedis (TP) is an infection of the feet caused by fungi. The infectious diseases caused by dermatophytes are mainly related to the enzymes produced by these fungi. Up to the now, the local 8-iso-prostaglandin F2α (8-iso-PGF2α), concentration as oxidative stress biomarker and trace elements status have not been published in patients with TP. The aim of this study is to evaluate the relationship between oxidative stress and trace elements (Cu, Zn, Se), and to evaluate the ratios of Cu/Zn and Cu/Se in this disorder.
  
  
  METHODS
  Forty-three consecutive patients with a diagnosis of unilateral interdigital TP were enrolled in this study. The samples were obtained by scraping the skin surface. 8-iso-PGF2α concentrations in scraping samples were determined by ELISA. In addition, the levels of Se, Zn and Cu in scraping samples were determined on flame and furnace atomic absorption spectrophotometer using Zeeman background correction.
  
  
  RESULTS
  Oxidative stress was confirmed by the significant elevation in 8-iso-PGF2α concentrations (p < 0.05). When compared to non-lesional area, Zn and Se levels were significantly lower on lesional area, whereas Cu levels was higher on the lesional area than the non-lesional area (p < 0.05). In addition, the correlation results of this study were firstly shown that there were significant and positive correlations between Cu and 8-iso-PGF2α parameters, but negative correlations between Se-Cu; Se-8-iso-PGF2α parameters in lesional area. Furthermore, the ratios of Cu/Zn and Cu/Se were significantly higher on the lesional area than the non-lesional area (p < 0.05). According to sex and fungal subtypes, there was no significant difference in the concentrations of 8-iso-PGF2α and trace elements in patients with TP (p > 0.05).
  
  
  CONCLUSIONS
  Our results showed that there is a possible link between oxidative stress (increased 8-iso-PGF2α concentrations) and imbalanced of trace elements status in lesional area of TP patients. The use of antifungal agents together with both Zn and Se drugs could be helpful in the both regression of disease and in shortening the duration of disease.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Cukurova University, 01330 Adana, Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Miraloglu
authors.affiliation: Department of Biochemistry, Sutcu Imam University, Faculty of Medicine, Kahramanmaras, Turkey.
authors.forename: Ergul Belge
authors.initials: EB
authors.lastname: Kurutas
authors.affiliation: Department of Dermatology, Sutcu Imam University, Faculty of Medicine, Kahramanmaras, Turkey.
authors.forename: Perihan
authors.initials: P
authors.lastname: Ozturk
authors.affiliation: Department of Dermatology, Fatih University, Faculty of Medicine, Istanbul, Turkey.
authors.forename: Ozer
authors.initials: O
authors.lastname: Arican
keywords: 8-iso-PGF2α; Oxidative stress; Tinea pedis; Trace elements