title: Photodynamic Graphene Quantum Dot: Reduction Condition Regulated Photoactivity and Size Dependent Efficacy.
abstract: Prequenching and selective activation of photosensitizer (PS) are highly desired in photodynamic therapy (PDT) to avoid off-target effect due to nonspecific activation and poor targeting selectivity of PS. In this study, nanographene materials as a unique π-conjugated planar system for electronic transfer were employed as the robust platform for temporarily quenching of PS. Photosensitizer chlorin e6 (Ce6) was integrated onto planar structure of graphene quantum dot (GQD) or graphene oxide (GO) via a reduction cleavable disulfide linker. The formed hybrid nanosystem displayed considerable fluorescence quenching and slight phototoxicity, even under the condition of light irradiation, while the photoactivity of PS could be selectively recovered in the presence of the reducing agent. Compared with graphene oxide system with larger size (around 200 nm), GQD nanosystem exhibited significantly improved tumor accumulation via enhanced permeation and retention effect (EPR effect). The in vivo study demonstrated extremely effective suppression of tumor growth for the group treated with the GQD nanosystem with cleavable linker, revealing the promising application of the presented novel strategy.
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine , Shanghai, People's Republic of China.
authors.forename: Dou
authors.initials: D
authors.lastname: Du
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine , Shanghai, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine , Shanghai, People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine , Shanghai, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine , Shanghai, People's Republic of China.
authors.forename: Yong Y
authors.initials: YY
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002735:Chlorophyllides; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008027:Light; D010087:Oxides / Q000737:chemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000627:therapeutic use; D011166:Porphyrins / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D002735:Chlorophyllides; D010087:Oxides; D017319:Photosensitizing Agents; D011166:Porphyrins; C062985:phytochlorin; D006108:Graphite
keywords: EPR effect; disulfide; graphene quantum dot; photoactivity; photodynamic therapy; redox-responsive