title: In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy.
abstract: Ionizing radiation (IR) injury is tissue damage caused by high energy electromagnetic waves such as X-ray and gamma ray. Diagnosis and treatment of IR injury are difficult due to its characteristics of clinically latent post-irradiation periods and the following successive and unpredictable inflammatory bursts. Skin is one of the many sensitive organs to IR and bears local injury upon exposure. Early-stage diagnosis of IR skin injury is essential in order to maximize treatment efficiency and to prevent the aggravation of IR injury. In this study, early-stage changes of the IR injured skin at the cellular level were characterized in an in vivo mouse model by two-photon microscopy (TPM). Various IR doses were applied to the mouse hind limbs and the injured skin regions were imaged daily for 6 days after IR irradiation. Changes in the morphology and distribution of the epidermal cells and damage of the sebaceous glands were observed before clinical symptoms. These results showed that TPM is sensitive to early-stage changes of IR skin injury and may be useful for its diagnosis.
authors.affiliation: Divison of Integrative Biosciences &Biotechnology, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Rep. of Korea.
authors.forename: Won Hyuk
authors.initials: WH
authors.lastname: Jang
authors.affiliation: National Radiation Emergency Medical Centre, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.
authors.forename: Sehwan
authors.initials: S
authors.lastname: Shim
authors.affiliation: Divison of Integrative Biosciences &Biotechnology, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Rep. of Korea.
authors.forename: Taejun
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Rep. of Korea.
authors.forename: Yeoreum
authors.initials: Y
authors.lastname: Yoon
authors.affiliation: Laboratory of Experimental Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.
authors.forename: Won-Suk
authors.initials: WS
authors.lastname: Jang
authors.affiliation: National Radiation Emergency Medical Centre, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.|Laboratory of Experimental Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.|Department of Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Myung
authors.affiliation: National Radiation Emergency Medical Centre, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.|Laboratory of Experimental Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.|Department of Pathology, Korea Cancer Centre Hospital, Korea Institute of Radiological &Medical Sciences (KIRAMS), 75 Nowon-ro, Nowon-gu, Seoul 01812, Rep. of Korea.
authors.forename: Sunhoo
authors.initials: S
authors.lastname: Park
authors.affiliation: Divison of Integrative Biosciences &Biotechnology, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Rep. of Korea.|Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Rep. of Korea.
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004817:Epidermis / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008853:Microscopy; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000473:pathology*; D011833:Radiation Injuries, Experimental; D011839:Radiation, Ionizing; D012627:Sebaceous Glands / Q000473:pathology / Q000528:radiation effects; D012871:Skin Diseases / Q000209:etiology* / Q000473:pathology*