title: Thiolated-2-methacryloyloxyethyl phosphorylcholine protected silver nanoparticles as novel photo-induced cell-killing agents.
abstract: Silver nanoparticles (AgNPs) have several medical applications as antimicrobial agents such as in drug delivery and cancer therapy. However, AgNPs are of limited use because of their toxicity, which may damage the surrounding healthy tissue. In this study, thiolated-2-methacryloyloxyethyl phosphorylcholine (MPC-SH) protected silver nanoparticles (MPC-AgNPs) are prepared as cell-killing agents under UV irradiation. MPC-AgNPs are characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and UV-visible spectrophotometry. The surface plasmon resonance (SPR) band of MPC-AgNPs is observed at 404 nm, and the average diameter of the particles is determined at 13.4 ± 2.2 nm through transmission electron microscopy (TEM) and at 18.4 nm (PDI=0.18) through dynamic light scattering (DLS). Cell viability in contact with MPC-AgNPs is relatively high, and MPC-AgNPs also exhibit a cell-killing effect under UV irradiation.
authors.affiliation: Graduate School of Science and Engineering, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan.
authors.forename: Arunee
authors.initials: A
authors.lastname: Sangsuwan
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan; ORDIST, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan.
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan; ORDIST, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan. Electronic address: yasu.bmt@kansai-u.ac.jp.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010316:Particle Size; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D056951:Photoelectron Spectroscopy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D014466:Ultraviolet Rays; D014961:X-Ray Diffraction
chemical_list: D008689:Methacrylates; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D010767:Phosphorylcholine; D012834:Silver; C070638:2-methacryloyloxyethyl phosphorylcholine
keywords: 2-Methacryloyloxyethyl phosphorylcholine; Biocompatibility; Cytotoxicity; Photo-induced cell-killing agents; Silver nanoparticles; Surface modification; Thiolene reaction