title: Expression and diagnosis of transient receptor potential vanilloid1 in urothelium of patients with overactive bladder.
abstract: This study was carried out to test expression of transient receptor potential vanilloid1 (TRPV1) in urothelium of female patients with overactive bladder (OAB) and explore clinical significance of TRPV1 in diagnosing female OAB. TRPV1 expression in urothelium of female OAB patients (n=21) and healthy females (n=9) was detected using Strept Avidin-Biotin Complex (SABC), an immunohistochemical method and image analysis system. Relative content of TRPV1 was expressed by average optical density (AOD) and was analyzed through data of urodynamics. Compared to TRPV1 expression in urothelium of healthy females (AOD 0.3658 ± 0.1009), TRPV1 expression in OAB patients was much higher (AOD 0.4834 ± 0.1252) and the difference was significant P less than 0.05. Observation and comparison in clinic of urodynamic parameters of female patients and healthy females revealed that the former had lower indexes with remarkable differences (P less than 0.05) such as Qmax, first desire volume (FDV), strong desire volume (SDV), maximum cyst capacity (MCC) and bladder compliance (BC). Thus high expression of TRPV1 in urothelium of female OAB patients is closely correlated to OAB occurrence, showing great importance of improved bladder sensitivity in female OAB occurrence mechanism.
authors.affiliation: Department of Urology, Yantai Hill Hospital of Yantai City, Shandong, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Department of Urology, Yantai Hill Hospital of Yantai City, Shandong, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Chu
authors.affiliation: Department of Urology, Yantai Hill Hospital of Yantai City, Shandong, China.
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Urology, Yantai Hill Hospital of Yantai City, Shandong, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Urology, First Peoples Hospital of Jining, Shandong, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Lv
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D050916:TRPV Cation Channels / Q000032:analysis* / Q000502:physiology; D053201:Urinary Bladder, Overactive / Q000209:etiology / Q000378:metabolism*; D019459:Urothelium / Q000737:chemistry*
chemical_list: D050916:TRPV Cation Channels; C494482:TRPV1 protein, human