title: The effect of phytosterol protects rats against 4-nitrophenol-induced liver damage.
abstract: We investigated the effect of phytosterol (PS) in regard to liver damage induced by 4-nitrophenol (PNP). Twenty rats were randomly divided into four groups (Control, PS, PNP, and PNP+PS). The PS and PNP+PS groups were pretreated with PS for one week. The PNP and PNP+PS groups were injected subcutaneously with PNP for 28 days. The control group received a basal diet and was injected with vehicle alone. Treatment with PS prevented the elevation of the total bilirubin levels, as well as an increase in serum alkaline transaminase and aspartate transaminase, which are typically caused by PNP-induced liver damage. Histopathologically showed that liver damage was significantly mitigated by PS treatment. However, there was no significant change in antioxidant enzyme activities, and the Nrf2-antioxidant system was not activated after treatment with PS. These results suggest that PS could mitigate liver damage induced by PNP, but does not enhance antioxidant capacity.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Meiyan
authors.initials: M
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Yansen
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China. Electronic address: chunmeili@njau.edu.cn.
authors.forename: ChunMei
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007279:Injections, Subcutaneous; D008297:Male; D051267:NF-E2-Related Factor 2; D009596:Nitrophenols / Q000633:toxicity*; D010840:Phytosterols / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000637:Transaminases / Q000097:blood*
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D009596:Nitrophenols; D010840:Phytosterols; D000637:Transaminases; D001663:Bilirubin; C024836:4-nitrophenol
keywords: 4-Nitrophenol; Liver; Oxidative stress; Phytosterol; Rat