title: MiR-328 suppresses the survival of esophageal cancer cells by targeting PLCE1.
abstract: Esophageal cancer (EC) is the sixth leading cause of death worldwide. Recent studies have highlighted the vital role of microRNAs (miRNAs) in EC development and diagnosis. In our study, qPCR analysis showed that miRNA-328 was expressed at significantly low levels in EC109 and EC9706 cells. The results also showed that overexpression of miR-328 by lentivirus-mediated gene transfer markedly inhibited cell proliferation and invasion, and enhanced apoptosis; whereas, inhibition of miR-328 significantly promoted cell proliferation and invasion, and suppressed apoptosis in EC109 and EC9706 cells. Dual-luciferase reporter assay confirmed that miR-328 directly targeted phospholipase C epsilon 1 (PLCE1) by binding to target sequences in the 3'-UTR. qPCR and Western blot analysis showed that the PLCE1 was overexpressed in EC109 and EC9706 cells. Additionally, we found that miR-328 overexpression decreased PLCE1 mRNA and protein levels, while miR-328 inhibition enhanced the PLCE1 expression. Further analysis showed that PLCE1 overexpression rescued the inhibitory effect of miR-328 on cell proliferation and invasion, and repressed the promotive effect of miR-328 on cell apoptosis. In conclusion, our results suggest that miR-328 suppresses the survival of EC cells by regulating PLCE1 expression, which might be a potential therapeutic method for EC.
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450014, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.affiliation: Department of Physiology and Neurobiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450014, PR China.
authors.forename: Zhongmian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: No.3, Kangfuqian Street, Department of Gastroenterology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, PR China; No.3, Kangfuqian Street, Medical Microecology and Clinical Nutrition Research Institute of Zhengzhou University, Zhengzhou, Henan, 450052, PR China. Electronic address: pengyuanzhengcn@163.com.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism*
chemical_list: C548170:MIRN328 microRNA, human; D035683:MicroRNAs; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon
keywords: Esophageal cancer (EC); PLCE1; miR-328