title: Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil.
abstract: The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in eight samples of different types of propolis (red, green and brown), collected from different regions in Brazil. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH and ABTS), Artepillin C, p-coumaric acid and antimicrobial activity against two bacteria were determined for all extracts. For the EtOH extracts, the anti-proliferative activity regarding the cell lines of B16F10, were also evaluated. Amongst the samples evaluated, the red propolis from the Brazilian Northeast (states of Sergipe and Alagoas) showed the higher biological potential, as well as the larger content of antioxidant compounds. The best results were shown for the extracts obtained through the conventional extraction method (EtOH). However, the highest concentrations of Artepillin C and p-coumaric acid were identified in the extracts from SCO2, indicating a higher selectivity for the extraction of these compounds. It was verified that the composition and biological activity of the Brazilian propolis vary significantly, depending on the type of sample and geographical area of collection.
authors.affiliation: Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil.|Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.
authors.forename: Bruna Aparecida Souza
authors.initials: BA
authors.lastname: Machado
authors.affiliation: Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.
authors.forename: Rejane Pina Dantas
authors.initials: RP
authors.lastname: Silva
authors.affiliation: Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.
authors.forename: Gabriele de Abreu
authors.initials: Gde A
authors.lastname: Barreto
authors.affiliation: Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.
authors.forename: Samantha Serra
authors.initials: SS
authors.lastname: Costa
authors.affiliation: Faculty of Pharmacy, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
authors.forename: Danielle Figuerêdo da
authors.initials: DF
authors.lastname: Silva
authors.affiliation: Faculty of Pharmacy, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
authors.forename: Hugo Neves
authors.initials: HN
authors.lastname: Brandão
authors.affiliation: Faculty of Pharmacy, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
authors.forename: José Luiz Carneiro da
authors.initials: JL
authors.lastname: Rocha
authors.affiliation: Technology Development Center, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Odir Antônio
authors.initials: OA
authors.lastname: Dellagostin
authors.affiliation: Biotechnology, University of Caixias do Sul, Caixias do Sul, Rio Grande do Sul, Brazil.
authors.forename: João Antônio Pegas
authors.initials: JA
authors.lastname: Henriques
authors.affiliation: Biotechnology, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Marcelo Andres
authors.initials: MA
authors.lastname: Umsza-Guez
authors.affiliation: Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil.
authors.forename: Francine Ferreira
authors.initials: FF
authors.lastname: Padilha
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001938:Brazil; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011422:Propionates; D011429:Propolis / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D003373:Coumaric Acids; D005419:Flavonoids; D010636:Phenols; D010666:Phenylpropionates; D011422:Propionates; D000431:Ethanol; C102259:artepillin C; D011429:Propolis; C495469:p-coumaric acid