title: Lipidomics Biomarkers of Diet-Induced Hyperlipidemia and Its Treatment with Poria cocos.
abstract: Hyperlipidemia is a major cause of atherosclerotic cardiovascular disease. Poria cocos (PC) is a medicinal product widely used in Asia. This study was undertaken to define the alterations of lipid metabolites in rats fed a high-fat diet to induce hyperlipidemia and to explore efficacy and mechanism of action of PC in the treatment of diet-induced hyperlipidemia. Plasma samples were then analyzed using UPLC-HDMS. The untreated rats fed a high-fat diet exhibited significant elevation of plasma triglyceride and total and low-density lipoprotein (LDL) cholesterol concentrations. This was associated with marked changes in plasma concentrations of seven fatty acids (palmitic acid, hexadecenoic acid, hexanoylcarnitine, tetracosahexaenoic acid, cervonoyl ethanolamide, 3-hydroxytetradecanoic acid, and 5,6-DHET) and five sterols [cholesterol ester (18:2), cholesterol, hydroxytestosterone, 19-hydroxydeoxycorticosterone, and cholic acid]. These changes represented disorders of biosynthesis and metabolism of the primary bile acids, steroids, and fatty acids and mitochondrial fatty acid elongation pathways in diet-induced hyperlipidemia. Treatment with PC resulted in significant improvements of hyperlipidemia and the associated abnormalities of the lipid metabolites.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University , Xi'an 710069, China.|Division of Nephrology and Hypertension, School of Medicine, University of California-Irvine , Irvine, California 92897, United States.
authors.forename: Hua
authors.initials: H
authors.lastname: Miao
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University , Xi'an 710069, China.
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: Division of Nephrology and Hypertension, School of Medicine, University of California-Irvine , Irvine, California 92897, United States.
authors.forename: Nosratola D
authors.initials: ND
authors.lastname: Vaziri
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University , Xi'an 710069, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Tang
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University , Xi'an 710069, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University , Xi'an 710069, China.
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.affiliation: Division of Nephrology and Hypertension, School of Medicine, University of California-Irvine , Irvine, California 92897, United States.
authors.forename: Mahyar
authors.initials: M
authors.lastname: Khazaeli
authors.affiliation: Division of Nephrology and Hypertension, School of Medicine, University of California-Irvine , Irvine, California 92897, United States.
authors.forename: Mehrdokht
authors.initials: M
authors.lastname: Tarbiat-Boldaji
authors.affiliation: Division of Nephrology and Hypertension, School of Medicine, University of California-Irvine , Irvine, California 92897, United States.
authors.forename: Leili
authors.initials: L
authors.lastname: Hatami
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University , Xi'an 710069, China.|Division of Nephrology and Hypertension, School of Medicine, University of California-Irvine , Irvine, California 92897, United States.
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000188:drug therapy*; D000960:Hypolipidemic Agents / Q000008:administration & dosage*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D055441:Poria / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D000960:Hypolipidemic Agents; D008077:Lipoproteins, LDL; D014280:Triglycerides; D002784:Cholesterol
keywords: Poria cocos; fatty acid metabolism; hyperlipidemia; lipidomics; mass spectrometry; ultraperformance liquid chromatography