title: Antimicrobial potential of commercial silver nanoparticles and the characterization of their physical properties toward probiotic bacteria isolated from fermented milk products.
abstract: The application of nanotechnology in the agriculture and food sector is relatively recent compared to its usage in drug delivery or pharmaceuticals. Therefore, this paper presents a study of the effect of silver nanoparticles on probiotic bacteria based on the example of Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12 and Streptococcus thermophilus ST-Y31 isolated from fermented milk products. Probiotic bacteria are one of the most crucial groups of bacteria for the food industry, because of their claimed health-promoting properties. Studies have shown that the type and concentration of silver nanoparticle solutions have a significant impact on the tested probiotic bacteria which are profitable for the digestive system. In the presence of all tested silver nanoparticles, St. thermophilus ST-Y31 growth was inhibited significantly by the dilution method as opposed to the disk-diffusion method. Both the disk-diffusion and the dilution methods showed no significant differences between L. acidophilus LA-5 and B. animalis subsp. lactis BB-12. The concentrations 2 μg mL(-1) and 0.25 μg mL(-1) had the highest antibacterial activity and statistically significant impacts on the tested probiotic strains. To our knowledge, this is the first report on potential antimicrobial effect of nanosilver against the health-promoting probiotic bacteria L. acidophilus LA-5, B. animalis subsp. lactis BB-12 and St. thermophilus ST-Y31 isolated from fermented milk products.
authors.affiliation: a Department of Functional Food, Ecological Food and Commodities , Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences , Warsaw , Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Mikiciuk
authors.affiliation: a Department of Functional Food, Ecological Food and Commodities , Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences , Warsaw , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Mikiciuk
authors.affiliation: a Department of Functional Food, Ecological Food and Commodities , Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences , Warsaw , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wrońska
authors.affiliation: b Department of Food Analysis , Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology , Warsaw , Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Szterk
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects; D043302:Cultured Milk Products / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000187:drug effects; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D019936:Probiotics; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D048249:Streptococcus thermophilus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Silver nanoparticles; antimicrobial effect; lactic acid bacteria; packaging; probiotic