title: MiR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein.
abstract: Nin one binding (NOB1) protein has been identified as an oncogene in various human cancers, including prostate cancer. MicroRNAs (miRs) have also been recognized as novel regulatory molecules of gene expression. The present study aimed to discover potential miRs that target NOB1 and regulate NOB1 expression in prostate cancer. miR-192, which is an important regulator of numerous cancers, was found to be significantly downregulated in prostate cancer cells. Moreover, we noted that miR-192 overexpression markedly inhibited the proliferation, colony‑forming ability, and migratory capacity of the prostate cancer cells. miR-192 overexpression also induced cell cycle arrest in the G1 phase, as shown by flow cytometry. Bioinformatics analysis results revealed that NOB1 was a possible candidate target gene of miR-192. This discovery was further verified through dual‑luciferase reporter assay, RT-qPCR, and western blot analysis. We suggest that miR-192 directly regulates the mRNA and protein expression of NOB1. Furthermore, miR-192 inhibited the expression of p38 mitogen-activated protein kinase. The results of our study indicated that miR-192 negatively regulated NOB1 expression and impaired the tumorigenicity of prostate cancer cells. Therefore, we suggest that targeting miR-192 and NOB1 is a novel strategy which will assist in the development of new therapeutics that will be used in the future to prevent and treat prostate cancer.
authors.affiliation: Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471002, P.R. China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Urology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471002, P.R. China.
authors.forename: Shuaiqi
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471002, P.R. China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471002, P.R. China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Hao
authors.affiliation: Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471002, P.R. China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Huo
mesh_terms: D063646:Carcinogenesis / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
chemical_list: C546783:MIRN192 microRNA, human; D035683:MicroRNAs; C506664:NOB1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D048051:p38 Mitogen-Activated Protein Kinases