title: Sialoglycoproteins prepared from the eggs of Carassius auratus prevent bone loss by inhibiting the NF-κB pathway in ovariectomized rats.
abstract: In this study, we investigated the improvement of osteoporosis by sialoglycoproteins isolated from the eggs of Carassius auratus (Ca-SGP) in ovariectomized rats. Ca-SGP was supplemented to ovariectomized Sprague-Dawley rats for 90 days. The results showed that Ca-SGP treatment remarkably prevented the reduction of bone mass, improved cancellous bone structure and biochemical properties. Ca-SGP also significantly decreased the serum contents of TRAP, Cath-K, MMP-9, DPD, CTX-1, Ca, and P. Mechanism investigation revealed that Ca-SGP significantly increased the OPG/RANKL ratio in mRNA expression, protein expression and serum content. Further research suggested that NF-κB signaling pathways were inhibited by suppressing the mRNA and protein expressions of NFATc1 and TRAF6, diminishing the mRNA expression and phosphorylation of NF-κB p65, three key transcription factors in NF-κB pathways. These results suggest that Ca-SGP can improve osteoporosis by inhibiting bone resorption via suppressing the activation of osteoclastogenesis related NF-κB pathways.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Xia
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Qingdao Central Hospital, Qingdao, Shandong Province 266003, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D000818:Animals; D005260:Female; D029941:Fish Proteins / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism; D010012:Osteogenesis; D010024:Osteoporosis / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology / Q000517:prevention & control; D010052:Ovariectomy; D010063:Ovum / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000378:metabolism*; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D016328:NF-kappa B; D012795:Sialoglycoproteins; D048029:TNF Receptor-Associated Factor 6; D051996:Transcription Factor RelA