title: Multidrug resistance in Clostridium perfringens isolated from diarrheal neonatal piglets in Thailand.
abstract: Clostridium perfringens causes diarrhea in neonatal piglets, thereby affecting commercial swine farming. The objective of this study was to determine the prevalence and characterize antimicrobial resistance in C. perfringens isolated from diarrheal neonatal piglets in Thailand. A total of 260 rectal swab samples were collected from 13 farms and were subjected to C. perfringens isolation. A total of 148 samples were PCR-positive for C. perfringens toxin genes, from which 122 were recovered. All isolates were cpb2-encoding C. perfringens type A and enterotoxin gene negative. Most of the isolates were susceptible to ampicillin, bacitracin, chlorotetracycline, doxycycline, and oxytetracycline with MIC50 values ranging from 0.32 to 8 μg/ml. The high resistance rates were observed for ceftiofur, enrofloxacin, erythromycin, lincomycin, and tylosin. Among resistant isolates, 82% were resistant to more than one type of antibiotics. The distinct pattern of multiple drug resistance in C. perfringens was observed in different regions, potentially reflecting the farm specific usage of these agents.
authors.affiliation: Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand; Microbiology Laboratory, Veterinary Diagnostic Center, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
authors.forename: Bhinyada
authors.initials: B
authors.lastname: Ngamwongsatit
authors.affiliation: Microbiology Laboratory, Veterinary Diagnostic Center, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
authors.forename: Wimonrat
authors.initials: W
authors.lastname: Tanomsridachchai
authors.affiliation: Faculty of Public Health, Thammasat University, Pathumthani, 12121, Thailand.
authors.forename: Orasa
authors.initials: O
authors.lastname: Suthienkul
authors.affiliation: Zoetis (Thailand) Limited, 323 United Building, 46th Floor, Silom Road, Bangkok, 10400, Thailand.
authors.forename: Supanee
authors.initials: S
authors.lastname: Urairong
authors.affiliation: Zoetis (Thailand) Limited, 323 United Building, 46th Floor, Silom Road, Bangkok, 10400, Thailand.
authors.forename: Wichian
authors.initials: W
authors.lastname: Navasakuljinda
authors.affiliation: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. Electronic address: tavan.jan@mahidol.ac.th.
authors.forename: Tavan
authors.initials: T
authors.lastname: Janvilisri
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003015:Clostridium Infections / Q000662:veterinary*; D003016:Clostridium perfringens / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D016000:Cluster Analysis; D003967:Diarrhea / Q000662:veterinary*; D024901:Drug Resistance, Multiple, Bacterial; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D013785:Thailand / Q000453:epidemiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotics; Clostridium perfringens; Diarrhea; Resistance; Swine