title: Altered miRNA expression in pulmonary sarcoidosis.
abstract: BACKGROUND
  miRNAs control important cellular functions including angiogenesis/angiostasis or fibrosis and reveal altered expression during pathological processes in the lung.
  
  
  METHODS
  The aim of the study was to investigate the expression of selected miRNAs (miR-let7f, miR-15b, miR-16, miR-20a, miR-27b, miR-128a, miR-130a, miR-192 miR-221, miR-222) in patients with pulmonary sarcoidosis (n = 94) and controls (n = 50). The expression was assessed by q-PCR in BALF cells and peripheral blood lymphocytes (PB lymphocytes). For statistical analysis, the Kruskal-Wallis test, Mann-Whitney U- test, Neuman-Keuls' multiple comparison test, and Spearman's rank correlation were used.
  
  
  RESULTS
  In BALF cells, significantly higher expression of miR-192 and miR-221 and lower expression of miR-15b were found in patients than controls. MiR-27b, miR-192 and miR-221 expression was significantly higher in patients without parenchymal involvement (stages I) than those at stages II-IV. Patients with acute disease demonstrated significantly higher miR-27b, miR-192 and miR-221 expression than those with insidious onset. For PB lymphocytes, patients demonstrated significantly greater miR-15b, miR-27b, miR-192, miR-221 and miR-222 expression, but lower miR-let7f and miR-130a expression, than controls. Stage I patients demonstrated significantly higher miR-16 and miR-15b expression than those in stages II-IV, and patients with the acute form demonstrated higher miR-130a and miR-15b expression. In BALF cells, miR-16 and miR-20a expression was significantly higher in patients with lung volume restriction, and miR-let7f was higher in the PB lymphocytes in patients with obturation. Several correlations were observed between the pattern of miRNA expression, lung function parameters and selected laboratory markers.
  
  
  CONCLUSIONS
  The obtained results suggest that the studied miRNAs play a role in the pathogenesis of sarcoidosis, and that some of them might have negative prognostic value.
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Kiszałkiewicz
authors.affiliation: Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, Kopcińskiego 22, 90-153, Lodz, Poland.
authors.forename: Wojciech J
authors.initials: WJ
authors.lastname: Piotrowski
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Pastuszak-Lewandoska
authors.affiliation: Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, Kopcińskiego 22, 90-153, Lodz, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Górski
authors.affiliation: Department of General and Oncological Pulmonology, 1st Chair of Internal Diseases, Medical University of Lodz, Kopcińskiego 22, 90-153, Lodz, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Antczak
authors.affiliation: Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, Kopcińskiego 22, 90-153, Lodz, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Górski
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Domańska-Senderowska
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Migdalska-Sęk
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Karolina H
authors.initials: KH
authors.lastname: Czarnecka
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Nawrot
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland. ewa.brzezianska@umed.lodz.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzeziańska-Lasota
mesh_terms: D000328:Adult; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D035683:MicroRNAs