title: Promicromonospora albasp. nov., an actinomycete isolated from the cuticle ofCamponotus japonicasMayr.
abstract: A novel actinomycete, designated strain 1C-HV12T, was isolated from the cuticle ofCamponotus japonicasMayr and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genusPromicromonosporaand that it forms a monophyletic clade with the closest relativesPromicromonospora umidemergensJCM 17975T(99.17 % 16S rRNA gene sequence similarity),Promicromonospora vindobonensisV45T(98.88 %) andPromicromonospora iranensisHM 792T(98.85 %). Moreover, morphological and chemotaxonomic properties of strain 1C-HV12Talso confirmed the affiliation of the isolate to the genusPromicromonospora. The peptidoglycan hydrolysates contained alanine, glutamic acid and lysine, and whole-cell sugars were galactose, glucose, rhamnose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The major menaquinones were MK-9(H4) and MK-9(H2). The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0and anteiso-C17 : 0. The DNA G+C content was 71.6 mol%. However, the low level of DNA-DNA relatedness and some phenotypic characteristics allowed the isolate to be differentiated from closely related species. Therefore, it is concluded that strain 1C-HV12Trepresents a novel species of the genusPromicromonospora, for which the namePromicromonospora albasp. nov. is proposed. The type strain is 1C-HV12T( = CGMCC 4.7283T= DSM 100490T).
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.|Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang