title: Influence of retinoic acid on mesenchymal stem cell differentiation in amyloid hydrogels.
abstract: This paper presents data related to the research article "Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation" [1]. Here we probed the collective influence of all-trans retinoic acid (RA) and substrate properties (amyloid hydrogel) on human mesenchymal stem cell (hMSC) differentiation. Stem cells were cultured on soft amyloid hydrogels [1], [2] in the presence and absence of matrix encapsulated RA. The cell morphology was imaged and assessed via quantification of circularity. Further immunostaining and quantitative real time PCR was used to quantify various markers of differentiation in the neuronal lineage.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, Maharashtra, India.
authors.forename: Reeba Susan
authors.initials: RS
authors.lastname: Jacob
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, Maharashtra, India; IITB Monash Research Academy, IIT Bombay, Mumbai, India.
authors.forename: Subhadeep
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, Maharashtra, India.
authors.forename: Dhiman
authors.initials: D
authors.lastname: Ghosh
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, Maharashtra, India.
authors.forename: Samir K
authors.initials: SK
authors.lastname: Maji
keywords: Amyloid hydrogel; Nanofibrils; Self-assembly; Stem cell; Tissue engineering; retinoic acid