title: Inositol-Requiring Enzyme 1-Dependent Activation of AMPK Promotes Brucella abortus Intracellular Growth.
abstract: AMP-activated protein kinase (AMPK) is a serine/threonine kinase that is well conserved during evolution. AMPK activation inhibits production of reactive oxygen species (ROS) in cells via suppression of NADPH oxidase. However, the role of AMPK during the process of Brucella infection remains unknown. Our data demonstrate that B. abortus infection induces AMPK activation in HeLa cells in a time-dependent manner. The known AMPK kinases LKB1, CAMKKβ, and TAK1 are not required for the activation of AMPK by B. abortus infection. Instead, this activation is dependent on the RNase activity of inositol-requiring enzyme 1 (IRE1). Moreover, we also found that B. abortus infection-induced IRE1-dependent activation of AMPK promotes B. abortus intracellular growth with peritoneal macrophages via suppression of NADPH-derived ROS production.
  
  
  OBJECTIVE
  Previous studies showed that B. abortus infection does not promote any oxidative burst regulated by NADPH oxidase. However, the underlying mechanism remains elusive. We report for the first time that AMPK activation caused by B. abortus infection plays important role in NADPH oxidase-derived ROS production.
authors.affiliation: Central Laboratory, The Second Hospital of Jilin University, Changchun, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Dong
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wei
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Wanchun
authors.initials: W
authors.lastname: Sun
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China Qishengpeng@yahoo.com.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Peng
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D002003:Brucella abortus / Q000254:growth & development / Q000502:physiology*; D004722:Endoribonucleases / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D017737:Macrophages, Peritoneal / Q000382:microbiology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C528229:ERN1 protein, human; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D004722:Endoribonucleases