title: In vitroscreening for anti-acetylcholiesterase, anti-oxidant, anti-glucosidase, anti-inflammatory and anti-bacterial effect of three traditional medicinal plants.
abstract: In this study we investigated the phytoconstituentsCalluna vulgaris,Ferula hermonisandTribulus terrestris, and then assessed their possible biological activities by using standard methods. A preliminary phytochemical investigation of the three extracts revealed the presence of alkaloids, flavonoids, proteins, lipids, phenolic compounds, saponins, sterols and amino acids. Three extracts showed anti-oxidant effect as they inhibited the 1,1-diphenyl-2-picryl hydrazyl (DPPH) oxidation and production of thiobarbituric acid reactive substances (TBARS). Moreover, three extracts showed anti-acetylcholiesterase (AChE) and this effect was concentration dependent.C. vulgariswas the most potent inhibitor of AChE. Furthermore, the three plant extracts had an inhibitory effect toward α-glucosidase. The inhibitory effect was concentration dependent and the most potent inhibitor for α-glucosidase was the extract fromT. terrestris. Calluna vulgarisshowed anti-inflammatory effect at tested concentrations while the other two extracts exhibited this effect only at concentration of 25 μg/mL. Finally,C. vulgarishad a significant effect against pathogenic bacteria (Agrobacterium tumefaciens,Erwiniasp.,Klebsiella pneumoniaandPseudomonas aeruginosa) in comparison to other extracts fromFerulasp., orTribulussp. In conclusion, all tested extracts could be promising sources for the treatment of diabetes, Alzheimer's disease, infectious diseases and oxidative stress related disorders because they are rich in phenols and flavonoids that give anti-oxidant molecules and produce an inhibitory effect against the tested enzymes.
authors.affiliation: Biochemistry Department, Faculty of Science, Alexandria University , Alexandria , Egypt.
authors.forename: Doaa A
authors.initials: DA
authors.lastname: Ghareeb
authors.affiliation: Plant and Microbiology Department, Faculty of Science, Alexandria University , Alexandria , Egypt.
authors.forename: Amani M D
authors.initials: AM
authors.lastname: ElAhwany
authors.affiliation: Chemistry Department, Faculty of Science, Alexandria University , Alexandria , Egypt.
authors.forename: Sherif M
authors.initials: SM
authors.lastname: El-Mallawany
authors.affiliation: Al-Leith University College, Umm Al-Qura University , Makkah , Saudi Arabia.
authors.forename: Ashraf A
authors.initials: AA
authors.lastname: Saif
keywords: Alzheimer treatment; Calluna vulgaris; Ferula hermonis; Tribulus terrestris; anti-diabetic