title: Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles.
abstract: OBJECTIVE
  The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles.
  
  
  METHODS
  We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles.
  
  
  RESULTS
  For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ(2) F:%σ(2) B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies.
  
  
  CONCLUSIONS
  These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.
authors.affiliation: Department of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA.
authors.forename: Keewan
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA. mbloom@albany.edu.|Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA. mbloom@albany.edu.
authors.forename: Michael S
authors.identifier: http://orcid.org/0000-0002-0028-5494
authors.initials: MS
authors.lastname: Bloom
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Victor Y
authors.initials: VY
authors.lastname: Fujimoto
authors.affiliation: Department of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA.
authors.forename: Erin M
authors.initials: EM
authors.lastname: Bell
authors.affiliation: Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA.
authors.forename: Recai M
authors.initials: RM
authors.lastname: Yucel
authors.affiliation: Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Browne
mesh_terms: D000328:Adult; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism
chemical_list: D008075:Lipoproteins, HDL
keywords: Biological variability; Follicular fluid (FF); High density lipoprotein (HDL); Human ovarian follicles; In vitro fertilization (IVF)