title: MRI monitoring of nanocarrier accumulation and release using Gadolinium-SPIO co-labelled thermosensitive liposomes.
abstract: Encapsulation of anticancer drugs in triggerable nanocarriers can beneficially modify pharmacokinetics and biodistribution of chemotherapeutic drugs, and consequently increase tumor drug concentration and efficacy, while reducing side effects. Thermosensitive liposomes release their contents triggered by hyperthermia, which can be, for example, precisely delivered using an MR Imaging-guided focused ultrasound procedure. In such a scenario, it is attractive to demonstrate the accumulation of liposomes before applying hyperthermia, as well as to document the release of liposome content using MRI. To address this need, thermosensitive liposomes were developed and characterized, which were doubly loaded by iron oxide nanoparticles and Gd-chelate, as opposed to loading with a single contrast agent. When intact, the transverse relaxivity of the liposomes is high allowing detection of carriers in tissue. After heating the longitudinal relaxivity steeply increases indicating release of the small molecular contents. By choosing the appropriate MR sequences, availability and release can be evaluated without interference of one contrast agent with the other. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: University Medical Center Utrecht, Department of Radiology, Imaging Division, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Cyril
authors.initials: C
authors.lastname: Lorenzato
authors.affiliation: University Medical Center Utrecht, Department of Radiology, Imaging Division, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Chris
authors.initials: C
authors.lastname: Oerlemans
authors.affiliation: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, 3584 CG, Utrecht, The Netherlands.
authors.forename: Merel
authors.initials: M
authors.lastname: van Elk
authors.affiliation: Biomolecular Imaging, Bijvoet Center, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: Willie J C
authors.initials: WJ
authors.lastname: Geerts
authors.affiliation: University Medical Center Utrecht, Department of Radiology, Imaging Division, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.|Institut de Mathématiques de Bordeaux, UMR 5251 CNRS/Université de Bordeaux, F-33400, Talence, France.
authors.forename: Baudouin
authors.initials: B
authors.lastname: Denis de Senneville
authors.affiliation: University Medical Center Utrecht, Department of Radiology, Imaging Division, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Chrit
authors.initials: C
authors.lastname: Moonen
authors.affiliation: University Medical Center Utrecht, Department of Radiology, Imaging Division, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Clemens
authors.initials: C
authors.lastname: Bos
mesh_terms: D003287:Contrast Media / Q000737:chemistry; D004337:Drug Carriers / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D065546:Drug Liberation / Q000528:radiation effects*; D005682:Gadolinium; D006358:Hot Temperature; D008081:Liposomes / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D000069453:Ultrasonic Waves
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D008081:Liposomes; D058185:Magnetite Nanoparticles; D005682:Gadolinium
keywords: High Intensity focused ultrasound (HIFU); drug delivery systems (DDS); magnetic resonance imaging (MRI); magnetoliposomes; relaxometry; thermosensitive liposomes (TSL)