title: Differential expression of six chicken genes associated with fatness traits in a divergently selected broiler population.
abstract: A genome-wide association study has shown a number of chicken (Gallus gallus) single nucleotide polymorphism (SNP) markers to be significantly associated with abdominal fat content in Northeast Agricultural University (NEAU) broiler lines selected divergently for abdominal fat content (NEAUHLF). The six significant SNPs are located in the kinase insert domain receptor (KDR), tumor suppressor candidate 3 (TUSC3), phosphoribosyl pyrophosphate amidotransferase (PPAT), exocyst complex component 1 (EXOC1), v-myb myeloblastosis viral oncogene homolog (avian)-like 2 (MYBL2) and KIAA1211 (undefined) genes. In this study, the expression levels of these genes were investigated in both abdominal fat and liver tissues using 32 14th generation chickens from the NEAUHLF. The levels of expression of KDR in abdominal fat and KDR and TUSC3 in liver differed significantly between the two lines. The expression level of KDR in the abdominal fat was significantly correlated with the abdominal fat weight (AFW) and abdominal fat percentage (AFP). The expression levels of KDR, TUSC3 and PPAT in liver were significantly correlated with AFW and AFP, indicating that the six genes, especially KDR and TUSC3, could be associated with fat traits in domestic chickens. This study could provide insight into the mechanisms underlying the formation of abdominal fat in chickens.
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Songsong
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Jin
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Luan
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yumao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Li
authors.initials: L
authors.lastname: Leng
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. Electronic address: shouzhiwang@126.com.
authors.forename: Shouzhi
authors.initials: S
authors.lastname: Wang
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D020869:Gene Expression Profiling / Q000379:methods / Q000662:veterinary*; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000379:methods* / Q000662:veterinary*; D005828:Genetics, Population / Q000379:methods; D005838:Genotype; D015810:Linkage Disequilibrium; D009929:Organ Size / Q000235:genetics; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide
chemical_list: D030161:Avian Proteins
keywords: Abdominal fat; Candidate genes; Chicken; Differential expression; Liver