title: Emerging therapy in arthritis: Modulation of markers of the inflammatory process.
abstract: The induction of tolerance has been proposed as a therapeutic strategy for arthritis aiming to decrease progression of the pathology, probably by promoting suppressor mechanisms of the autoimmune response. This work aimed to confirm whether the treatment with vitamin D3 could synergize oral tolerance induced by hydrolyzed collagen peptides, in our experimental model of antigen induced arthritis in New Zealand rabbits. Clinical observation of the phenomenon indicates that simultaneous treatment with hydrolyzed collagen peptides and vitamin D3 was beneficial when compared with no treatment, for arthritic animals, and for arthritic animals that received treatment with only hydrolyzed collagen peptides or vitamin D3. Treatment with hydrolyzed collagen peptides caused diminished proinflammatory cytokine levels, an effect synergized significantly by the simultaneous treatment with vitamin D3. The anatomical-pathological studies of the animals that received both treatments simultaneously showed synovial tissues without lymphocytic and plasma cell infiltrates, and without vascular proliferation. Some of the synovial tissue of the animals of these groups showed a slight decrease in Galectin-3 expression. We propose that simultaneous oral treatment with vitamin D3 and hydrolyzed collagen peptides could increase the immunoregulatory effect on the process of previously triggered arthritis. We used articular cartilage hydrolysate and not collagen II because peptides best expose antigenic determinants that could induce oral tolerance. Oral tolerance may be considered in the design of novel alternative therapies for autoimmune disease and we have herein presented novel evidence that the simultaneous treatment with vitamin D3 may synergize this beneficial effect.
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: P A
authors.initials: PA
authors.lastname: Mortarino
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: D P
authors.initials: DP
authors.lastname: Goy
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: D B
authors.initials: DB
authors.lastname: Abramson
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Cabello
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: G E
authors.initials: GE
authors.lastname: Bumaguin
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: E J
authors.initials: EJ
authors.lastname: Vitelli
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Toledo
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Sarrio
authors.affiliation: INSTITUTO De Inmunología Clínica Y Experimental De Rosario (IDICER-CONICET), Rosario, Argentina.
authors.forename: S M
authors.initials: SM
authors.lastname: Pezzotto
authors.affiliation: School of Dentistry, Department of Morphology, Physiology and Basic Pathology, University of São Paulo, Brazil.
authors.forename: J P
authors.initials: JP
authors.lastname: Mardegan Issa
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.
authors.forename: G R
authors.initials: GR
authors.lastname: Cointry
authors.affiliation: Laboratorio De Biología Osteoarticular, Ingeniería Tisular Y Terapias Emergentes (LABOATEM), Facultad De Ciencias Médicas, Universidad Nacional De Rosario, Santa Fe, Argentina.|Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes (LABOATEM), Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Argentina and Consejo de Investigaciones de la Universidad Nacional de Rosario (CIUNR)-CONICET, Rosario, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Feldman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000276:immunology*; D002762:Cholecalciferol / Q000008:administration & dosage*; D003094:Collagen / Q000737:chemistry; D016207:Cytokines / Q000276:immunology; D005260:Female; D006801:Humans; D010455:Peptides / Q000008:administration & dosage*; D011817:Rabbits; D013583:Synovial Membrane / Q000276:immunology
chemical_list: D016207:Cytokines; D010455:Peptides; D002762:Cholecalciferol; D003094:Collagen
keywords: arthritis; immunomodulation; microscopy research; oral tolerance; vitamin D