title: [HPLC-PDA-CAD Fingerprints of Salt Anemarrhenae Rhizoma].
abstract: OBJECTIVE
  To establish the HPLC-PDA-CAD fingerprints and to determine its seven main constituents so as to provide a reliable evidence for the scientific evaluation and quality control of salt Anemarrhenae Rhizoma.
  
  
  METHODS
  The chromatographic fingerprint was obtained with Thermo Hypersil C18 column (250 mm x 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water; The CAD parameters were pressure of 241. 3 kPa, filter of high and range of 200 pA. The detection wavelength of PDA was set at 258 nm.
  
  
  RESULTS
  The common mode of HPLC-PDA-CAD fingerprint of salt Anemarrhenae Rhizoma was set up. There were 5 PDA and 12 CAD common peaks in the fingerprints. Timosaponin B II, anemarsaponin E, timosaponin B III, timosaponin A III, neomangiferin, mangiferin and baohuoside I were identified in fingerprints and determined.
  
  
  CONCLUSIONS
  The established HPLC-PDA-CAD fingerprint method is accurate, reliable, and has a good reproducibility and precision, which can be used for the quality control of salt Anemarrhenae Rhizoma.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Ze-bi
authors.initials: ZB
authors.lastname: Song
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal; D064209:Phytochemicals / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D064209:Phytochemicals; D012503:Saponins; D013256:Steroids; C543146:timosaponin AIII; C523027:timosaponin B-II