title: Streptomyces daqingensissp. nov., isolated from saline-alkaline soil.
abstract: A novel strain of actinobacteria, designated strain NEAU-ZJC8T, was isolated from a saline-alkaline soil collected from Heilongjiang Province, north-east China and characterized using a polyphasic approach. Strain NEAU-ZJC8Texhibited morphological, cultural and chemotaxonomic features consistent with its classification as representing a member of the genusStreptomyces. Growth occurred at 16-35 °C, pH 5.0-11.0 and in the presence of 0-11 % (w/v) NaCl. The cell-wall peptidoglycan contained ll-diaminopimelic acid and glycine. Whole-cell hydrolysates mainly contained glucose, galactose and ribose. Predominant menaquinones were MK-9(H6), MK-9(H4) and MK-9(H8). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, iso-C15 : 0, anteiso-C17 : 0and C16 : 0. The G+C content of the DNA was 72.7 mol%. A phylogenetic tree based on 16S rRNA sequences showed that strain NEAU-ZJC8Tformed a distinct clade within the genusStreptomycesand was closely related toStreptomyces haliclonaeDSM 41970T(98.56 % similarity) andStreptomyces marinusNBRC 105047T(97.96 % similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ZJC8Tcould be distinguished from its closest phylogenetic relatives. Therefore, strain NEAU-ZJC8Trepresents a novel species of the genusStreptomyces, for which the nameStreptomyces daqingensissp. nov. is proposed. The type strain is NEAU-ZJC8T( = CGMCC 4.7178T= JCM 30057T).
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Pan
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Hairong
authors.initials: H
authors.lastname: He
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Yuejing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,PR China.|Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University,No. 59 Mucai Street, Xiangfang District, Harbin 150030,PR China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang