title: Accurate concentration control of mitochondria and nucleoids.
abstract: All cellular materials are partitioned between daughters at cell division, but by various mechanisms and with different accuracy. In the yeast Schizosaccharomyces pombe, the mitochondria are pushed to the cell poles by the spindle. We found that mitochondria spatially reequilibrate just before division, and that the mitochondrial volume and DNA-containing nucleoids instead segregate in proportion to the cytoplasm inherited by each daughter. However, nucleoid partitioning errors are suppressed by control at two levels: Mitochondrial volume is actively distributed throughout a cell, and nucleoids are spaced out in semiregular arrays within mitochondria. During the cell cycle, both mitochondria and nucleoids appear to be produced without feedback, creating a net control of fluctuations that is just accurate enough to avoid substantial growth defects.
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Rishi
authors.initials: R
authors.lastname: Jajoo
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Yoonseok
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Dann
authors.initials: D
authors.lastname: Huh
authors.affiliation: Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA.
authors.forename: Matheus P
authors.initials: MP
authors.lastname: Viana
authors.affiliation: Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA.
authors.forename: Susanne M
authors.initials: SM
authors.lastname: Rafelski
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Springer
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. johan_paulsson@harvard.edu.
authors.forename: Johan
authors.initials: J
authors.lastname: Paulsson
mesh_terms: D002453:Cell Cycle; D048750:Cell Nucleus Division / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology / Q000648:ultrastructure; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D053142:Mitochondrial Size; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology; D012568:Schizosaccharomyces / Q000166:cytology / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C112212:Pom1 protein, S pombe