title: Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E2 Generation in LPS-stimulated RAW264.7 Cells.
abstract: Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE2) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE2 generation via suppression of iNOS and COX-2 expression.
authors.affiliation: Department of Dental Hygiene, Gwang Yang Health College, Gwangyang 57764, Korea.; College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 28160, Korea.
authors.forename: Yu Yeon
authors.initials: YY
authors.lastname: Jung
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 28160, Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 28160, Korea.
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Han
authors.affiliation: Department of Food Science and Nutrition, College of Natural Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Park
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 28160, Korea.
authors.forename: Dong Ju
authors.initials: DJ
authors.lastname: Son
keywords: COX-2; Inflammation; Ixeris dentate Nakai; Nitric oxide; Prostaglandin E2; iNOS