title: Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.
abstract: It is well established that topographical features modulate cell behaviour, including cell morphology, proliferation and differentiation. To define the effects of topography on human induced pluripotent stem cells (iPSC), we plated cells on a topographical library containing over 1000 different features in medium lacking animal products (xeno-free). Using high content imaging, we determined the effect of each topography on cell proliferation and expression of the pluripotency marker Oct4 24 h after seeding. Features that maintained Oct4 expression also supported proliferation and cell-cell adhesion at 24 h, and by 4 days colonies of Oct4-positive, Sox2-positive cells had formed. Computational analysis revealed that small feature size was the most important determinant of pluripotency, followed by high wave number and high feature density. Using this information we correctly predicted whether any given topography within our library would support the pluripotent state at 24 h. This approach not only facilitates the design of substrates for optimal human iPSC expansion, but also, potentially, identification of topographies with other desirable characteristics, such as promoting differentiation.
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, King's College London, 28th Floor, Tower Wing, Guy's Hospital, Great Maze Pond, London SE1 9RT, United Kingdom.
authors.forename: Andreas
authors.initials: A
authors.lastname: Reimer
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, Enschede, The Netherlands.|Merln Institute for Technology-inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.
authors.forename: Aliaksei
authors.initials: A
authors.lastname: Vasilevich
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, Enschede, The Netherlands.
authors.forename: Frits
authors.initials: F
authors.lastname: Hulshof
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, King's College London, 28th Floor, Tower Wing, Guy's Hospital, Great Maze Pond, London SE1 9RT, United Kingdom.
authors.forename: Priyalakshmi
authors.initials: P
authors.lastname: Viswanathan
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, Enschede, The Netherlands.|Materiomics b.v., Maastricht, The Netherlands.|Merln Institute for Technology-inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.
authors.forename: Clemens A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, Enschede, The Netherlands.|Materiomics b.v., Maastricht, The Netherlands.|Merln Institute for Technology-inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, King's College London, 28th Floor, Tower Wing, Guy's Hospital, Great Maze Pond, London SE1 9RT, United Kingdom.
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Watt
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000235:genetics*
chemical_list: D050814:Octamer Transcription Factor-3