title: Energy driven cascade recognition for selective detection of nucleic acids with high discrimination factor at room temperature.
abstract: In this article, we demonstrated a cascade recognition strategy for the detection of single strand nucleic acid with high discrimination factor at room temperature. The cascade recognition strategy contains a toehold mediated strand displacement and a double-toehold mediated double strand displacement reaction, thus enable the high ability to discern point mutation of target. The discrimination factor of the model target is between 45 and 109, with the medium of 70. This strategy is homogeneous, easy operation, enzyme-free, isothermal, and can be easily adapted to high-throughput devices without the need of designing complicated instruments.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Jun Long
authors.initials: JL
authors.lastname: Li
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yao
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yin
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Clinical Laboratory, Chongqing Municipal Jiulongpo District the First People's Hospital, Chongqing 400050, PR China.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: guomingxie@cqmu.edu.cn.
authors.forename: Guoming
authors.initials: G
authors.lastname: Xie
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D004247:DNA
keywords: Cascade recognition strategy; DNA; Single-base mutation; Toehold-mediated strand displacement reaction