title: Comments on Scheffler et al. Cytotoxic Evaluation of E-Liquid Aerosol using Different Lung Derived Cell Models. Int. J. Environ. Res. Public Health, 2015, 12, 12466-12474.
abstract: There is merit in considering a simple toxicological screening method that evaluates the total cytotoxic potential of e-liquids or electronic cigarettes (ECs) aerosol emissions in one single testing. However, there is growing confusion, with several researchers endorsing their personal solution to the problem. Here, we discuss as an example the recent paper by Scheffler and colleagues, in which the authors suggest that more relevant and well differentiated cell lines from human airways could be the most suitable candidates for toxicological evaluation of ECs aerosol emissions. We advance recommendations for validated protocols and advocate for an international coordinated effort aimed at establishing consensus on methodology.
authors.affiliation: Dipartimento di Medicina Clinica e Sperimentale, Università di Catania, Via Palermo 636, 95121 Catania, Italy. polosa@unict.it.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Polosa
authors.affiliation: Dipartimento di Medicina Clinica e Sperimentale, Università di Catania, Via Palermo 636, 95121 Catania, Italy. mascaru@unict.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Caruso
authors.affiliation: Dipartimento di Scienze Biomediche e Biotecnologiche, V.le A. Doria 6, 95125 Catania, Italy. fguarin@unict.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Guarino
mesh_terms: D066300:Electronic Nicotine Delivery Systems / Q000009:adverse effects*; D006801:Humans; D012906:Smoke / Q000009:adverse effects*; D023382:Toxicity Tests, Acute / Q000379:methods*; D062789:Tobacco Products
chemical_list: D012906:Smoke
keywords: cytotoxicity; electronic cigarette; tobacco product directive; vapour emission