title: Expression profile of FGF receptors in preimplantation ovine embryos and the effect of FGF2 and PD173074.
abstract: Fibroblast growth factors (FGFs) and their receptors (FGFRs) are increasingly recognized as important regulators of embryo development in mammals. This study investigated the importance of FGF signaling during in vitro development of ovine embryo. The mRNAs of four FGFR subtypes were detected throughout preimplantation development of in vitro fertilized (IVF) embryos, peaked in abundance at the morula stage, and decreased significantly at the blastocyst stage. To gain insight into the role of these mRNAs in embryo development, IVF embryos were cultured in the presence of FGF2 (100 or 500 ng/ml: beginning from days 1 or 4 to 7) or PD173074 (1 µM: beginning from days 1 to 7) as usual treatments for activation or inhibition of FGFRs, respectively. FGF2-supplementation did not affect the percentage of embryos that developed to the blastocyst, blastocyst cell count and the proportion of cells allocated in inner cell mass (ICM) and trophectoderm (TE) compared to control (p > 0.05). Also, increasing the dosage or duration of FGF2 treatment did not significantly alter blastocyst yield or differential cell count (p > 0.05). PD173074-mediated inhibition of FGFRs did not significantly affect blastocyst yield (p > 0.05). Assessment of expression profiles of lineage-associated markers revealed that FGF2 (500 ng/ml) supplementation: (i) significantly increased expression of putative hypoblast marker (GATA4), (ii) significantly decreased expression of putative epiblast (EPI) marker (NANOG) and (iii) did not change TE markers (CDX2 and IFNT) and pluripotency makers (OCT4, SOX2 and REX1). In summary, FGF2-mediated activation of FGFRs may promote a switch in transcriptional profile of ovine ICM from EPI- to hypoblast-associated gene expression.
authors.affiliation: a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran .|b Department of Animal Science , College of Agriculture, Isfahan University of Technology , Isfahan , Iran , and.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Moradi
authors.affiliation: b Department of Animal Science , College of Agriculture, Isfahan University of Technology , Isfahan , Iran , and.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Riasi
authors.affiliation: a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran .
authors.forename: Somayyeh
authors.initials: S
authors.lastname: Ostadhosseini
authors.affiliation: a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran .
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hajian
authors.affiliation: a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran .
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran .
authors.forename: Pouria
authors.initials: P
authors.lastname: Hosseinnia
authors.affiliation: a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran .|c Department of Embryology , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , ACECR , Tehran , Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D050991:GATA4 Transcription Factor / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D018398:Homeodomain Proteins / Q000096:biosynthesis; D008297:Male; D011743:Pyrimidines / Q000494:pharmacology*; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051499:Receptor, Fibroblast Growth Factor, Type 4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D012756:Sheep
chemical_list: D050991:GATA4 Transcription Factor; D018398:Homeodomain Proteins; C115711:PD 173074; D011743:Pyrimidines; D016222:Fibroblast Growth Factor 2; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D051498:Receptor, Fibroblast Growth Factor, Type 3; D051499:Receptor, Fibroblast Growth Factor, Type 4
keywords: FGF2; FGFR; epiblast; hypoblast; ovine embryo