title: Ligand-lipid and ligand-core affinity control the interaction of gold nanoparticles with artificial lipid bilayers and cell membranes.
abstract: Interactions between nanoparticles (NPs) and biomembranes depend on the physicochemical properties of the NPs, such as size and surface charge. Here we report on the size-dependent interaction of gold nanoparticles (AuNPs), stabilized with ligands differing in charge, i.e. sodium 3-(diphenylphosphino)benzene sulfonate (TPPMS) and sodium 3,3',3″-triphenylphosphine sulfonate (TPPTS), respectively, with artificial membranes (black lipid membranes; BLMs) and HeLa cells. The TPPTS-stabilized AuNPs affect BLMs at lower size than TPPMS-stabilized ones. On HeLa cells we found decreasing cytotoxicity with increasing particle size, however, with an overall lower cytotoxicity for TPPTS-stabilized AuNPs. We attribute size-dependent BLM properties as well as reduced cytotoxicity of TPPTS-stabilized AuNPs to weaker shielding of the AuNP core when stabilized with TPPTS. We hypothesize that the partially unshielded hydrophobic gold core can embed into the hydrophobic membrane interior. Thereby we demonstrate that ligand-dependent cytotoxicity of NP can occur even when the NPs are not translocated through the membrane.
  
  
  
  The use of nanoparticles (NPs) in the clinical setting means that there will be interactions between NPs and cell membranes. The authors investigated the underlying processes concerning cellular uptake and potential toxicity of gold nanoparticles (AuNPs) using particles with ligands different sizes and charges. The findings should further enhance existing knowledge on future design of safer NPs in the clinic.
authors.affiliation: Institute of Inorganic Chemistry, RWTH Aachen University, Aachen, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Broda
authors.affiliation: Institute of Nanotechnology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Setzler
authors.affiliation: Institute of Inorganic Chemistry, RWTH Aachen University, Aachen, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Leifert
authors.affiliation: Institute for Laboratory Animal Science, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Steitz
authors.affiliation: Rudolf Virchow Center, University of Würzburg, Würzburg, Germany; Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Benz
authors.affiliation: Institute of Inorganic Chemistry, RWTH Aachen University, Aachen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Simon
authors.affiliation: Institute of Nanotechnology, Karlsruhe Institute of Technology, Karlsruhe, Germany. Electronic address: wolfgang.wenzel@kit.edu.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wenzel
mesh_terms: D002462:Cell Membrane; D006046:Gold; D006801:Humans; D008051:Lipid Bilayers; D053768:Metal Nanoparticles; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D006046:Gold
keywords: Artificial black lipid membranes; Colloidal synthesis; Electrophysiology; Gold nanoparticles; Nanotoxicity; Size and surface charge effect