title: Using hydrogen deuterium exchange mass spectrometry to engineer optimized constructs for crystallization of protein complexes: Case study of PI4KIIIβ with Rab11.
abstract: The ability of proteins to bind and interact with protein partners plays fundamental roles in many cellular contexts. X-ray crystallography has been a powerful approach to understand protein-protein interactions; however, a challenge in the crystallization of proteins and their complexes is the presence of intrinsically disordered regions. In this article, we describe an application of hydrogen deuterium exchange mass spectrometry (HDX-MS) to identify dynamic regions within type III phosphatidylinositol 4 kinase beta (PI4KIIIβ) in complex with the GTPase Rab11. This information was then used to design deletions that allowed for the production of diffraction quality crystals. Importantly, we also used HDX-MS to verify that the new construct was properly folded, consistent with it being catalytically and functionally active. Structures of PI4KIIIβ in an Apo state and bound to the potent inhibitor BQR695 in complex with both GTPγS and GDP loaded Rab11 were determined. This hybrid HDX-MS/crystallographic strategy revealed novel aspects of the PI4KIIIβ-Rab11 complex, as well as the molecular mechanism of potency of a PI4K specific inhibitor (BQR695). This approach is widely applicable to protein-protein complexes, and is an excellent strategy to optimize constructs for high-resolution structural approaches.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, British Columbia, V8P 5C2, Canada.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Fowler
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, British Columbia, V8P 5C2, Canada.
authors.forename: Jacob A
authors.initials: JA
authors.lastname: McPhail
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, British Columbia, V8P 5C2, Canada.
authors.forename: Meredith L
authors.initials: ML
authors.lastname: Jenkins
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH, United Kingdom.
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Masson
authors.affiliation: Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco (UCSF), California, 94158.
authors.forename: Florentine U
authors.initials: FU
authors.lastname: Rutaganira
authors.affiliation: Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco (UCSF), California, 94158.
authors.forename: Kevan M
authors.initials: KM
authors.lastname: Shokat
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH, United Kingdom.
authors.forename: Roger L
authors.initials: RL
authors.lastname: Williams
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, British Columbia, V8P 5C2, Canada.
authors.forename: John E
authors.initials: JE
authors.lastname: Burke
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D020691:rab GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C581087:PI4KB protein, human
keywords: HDX-MS; Rab11; X-ray crystallography; hydrogen deuterium exchange mass spectrometry; lipid signaling; phosphatidylinositol 4 kinase; phosphoinositide signaling; structural biology