title: Changes in immunoexpression of p53, Ki-67, Ets-1, APAF-1 and PTEN in serrated and conventional colon adenomas.
abstract: The balance between apoptosis and proliferation is tipped towards a decrease of apoptosis as the colonocyte progresses in the adenoma to carcinoma sequence of colon carcinogenesis. According to literature data, proteins like p53, Ki-67, APAF-1, Ets-1, PTEN contribute to inhibition of apoptosis and stimulation of proliferation.
  
  
  OBJECTIVE
  Considering the complex interference among colorectal carcinogenetic mechanisms, our aim was to study the markers Ets-1 and APAF-1 relative to p53, Ki-67 and PTEN expression in colon adenomas/polyps (A/P).
  
  
  METHODS
  We performed immunohistochemistry on 99 colon A/P cases from the material of the Department of Pathology, Emergency County Hospital of Tirgu Mures, Romania. Secondary EnVision Flex/HRP (Horseradish peroxidase) (20 minutes) was used for signal amplification.
  
  
  RESULTS
  The majority of A/P show increased Ki-67, p53, Ets-1 expression, decreased APAF-1 expression and preserved PTEN expression. p53, Ki-67, Ets-1 and APAF-1 demonstrated statistically significant correlations with histological type and grade of dysplasia. We also observed that expression of these proteins in the intestinal crypts has a typical distribution according to histological type and grade of dysplasia.
  
  
  CONCLUSIONS
  In case of hyperplastic polyps APAF-1 expression decreases as p53 and Ki-67 expression increases, followed by a decrease in PTEN expression in serrated adenomas, and an increase of Ets-1 expression in conventional adenomas.
authors.affiliation: Department of Anatomy and Embryology, University of Medicine and Pharmacy of Tirgu Mures, Romania; deneslorand@gmail.com.
authors.forename: Zsuzsánna
authors.initials: Z
authors.lastname: Pap
authors.forename: Izabella Ágota
authors.initials: IÁ
authors.lastname: Ilyés
authors.forename: Simona Liliana
authors.initials: SL
authors.lastname: Mocan
authors.forename: Lóránd
authors.initials: L
authors.lastname: Dénes
authors.forename: Monica Cristina
authors.initials: MC
authors.lastname: Muică Nagy-Bota
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Pávai
authors.forename: Annamária
authors.initials: A
authors.lastname: Szántó
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053477:Apoptotic Protease-Activating Factor 1 / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D050784:Proto-Oncogene Protein c-ets-1 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D053477:Apoptotic Protease-Activating Factor 1; C494041:ETS1 protein, human; D019394:Ki-67 Antigen; D050784:Proto-Oncogene Protein c-ets-1; D016159:Tumor Suppressor Protein p53; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human