title: Core-shell microcapsules of solid lipid nanoparticles and mesoporous silica for enhanced oral delivery of curcumin.
abstract: Newly designed microcapsules (MC) combining a core of solid lipid nanoparticle (SLN) and a mesoporous silica shell have been developed and explored as oral delivery system of curcumin (CU). CU-loaded MC (MC-CU) are 2 μm sized and have a mesoporous silica shell of 0.3 μm thickness with a wormlike structure as characterized by small angle X-ray scattering (SAXS), nitrogen adsorption/desorption and transmission electron microscopy (TEM) measurements. It was found that SLN acts as reservoir of curcumin while the mesoporous shell insures the protection and the controlled release of the drug. MC-CU displayed a pH-dependent in vitro release profile with marked drug retention at pH 2.8. Neutral red uptake assay together with confocal laser scanning microscopy (CLSM) showed a good cell tolerance to MC-CU at relatively high concentration of inert materials. Besides, the cell-uptake test revealed that fluorescent-MC were well internalized into Caco-2 cells, confirming the possibility to use MC for gut cells targeting. These findings suggest that organic core-silica shell microcapsules are promising drug delivery systems with enhanced bioavailability for poorly soluble drugs.
authors.affiliation: SRSMC, UMR 7565, Université de Lorraine-CNRS, F-54506 Vandœuvre-lès-Nancy, France.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: SRSMC, UMR 7565, Université de Lorraine-CNRS, F-54506 Vandœuvre-lès-Nancy, France. Electronic address: roudayna.diab@univ-lorraine.fr.
authors.forename: Roudayna
authors.initials: R
authors.lastname: Diab
authors.affiliation: CITHEFOR, EA 3452, Université de Lorraine, F-54000 Nancy, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Joubert
authors.affiliation: SRSMC, UMR 7565, Université de Lorraine-CNRS, F-54506 Vandœuvre-lès-Nancy, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Canilho
authors.affiliation: SRSMC, UMR 7565, Université de Lorraine-CNRS, F-54506 Vandœuvre-lès-Nancy, France. Electronic address: andreea.pasc@univ-lorraine.fr.
authors.forename: Andreea
authors.initials: A
authors.lastname: Pasc
mesh_terms: D000284:Administration, Oral; D018938:Caco-2 Cells; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods; D065546:Drug Liberation; D004705:Endocytosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D002214:Capsules; D008055:Lipids; D012822:Silicon Dioxide; D003474:Curcumin
keywords: Bioavailability; Caco-2 cells; Core–shell mesoporous silica; Curcumin