title: Isolation of a lytic bacteriophage against virulent Aeromonas hydrophila from an organized equine farm.
abstract: A bacteriophage (VTCCBPA6) against a pathogenic strain of Aeromonas hydrophila was isolated from the sewage of an organized equine breeding farm. On the basis of TEM analysis, phage belonged to family Myoviridae. PCR amplification and sequence analysis of gp23 gene (encoding for major capsid protein) revealed phylogenetic resemblance to T4 like virus genus. Protein profiling by SDS-PAGE also indicated its resemblance to T4 like phage group. However, the comparison of its gp23 gene sequence with previously reported phages showed similarity with T4-like phages infecting Enterobacteriaceae instead of Aeromonas spp. Thus, to our knowledge, this report points toward the fact that a novel/evolved phage might exist in equine environment against A. hydrophila, which can be potentially used as a biocontrol agent.
authors.affiliation: Veterinary Type Culture Collection, Bagla Road, Hisar, 125001, India.
authors.forename: Taruna
authors.initials: T
authors.lastname: Anand
authors.affiliation: Veterinary Type Culture Collection, Bagla Road, Hisar, 125001, India.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Vaid
authors.affiliation: Veterinary Type Culture Collection, Bagla Road, Hisar, 125001, India.
authors.forename: Bidhan Ch
authors.initials: BCh
authors.lastname: Bera
authors.affiliation: ICAR - National Research Centre on Equines, Sirsa Road, Hisar, India.
authors.forename: Jitender
authors.initials: J
authors.lastname: Singh
authors.affiliation: Veterinary Type Culture Collection, Bagla Road, Hisar, 125001, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Barua
authors.affiliation: ICAR - National Research Centre on Equines, Sirsa Road, Hisar, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Virmani
authors.affiliation: ICAR - National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.
authors.forename: K
authors.initials: K
authors.lastname: Rajukumar
authors.affiliation: Veterinary Type Culture Collection, Bagla Road, Hisar, 125001, India.
authors.forename: Neeraj Kumar
authors.initials: NK
authors.lastname: Yadav
authors.affiliation: Veterinary Type Culture Collection, Bagla Road, Hisar, 125001, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Nagar
authors.affiliation: ICAR - Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: Raj K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: ICAR - National Research Centre on Equines, Sirsa Road, Hisar, India.
authors.forename: B N
authors.initials: BN
authors.lastname: Tripathi
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity / Q000821:virology*; D000818:Animals; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D036022:Capsid Proteins / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D000072480:Farms; D016679:Genome, Viral; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000628:therapy / Q000662:veterinary; D006734:Horse Diseases / Q000382:microbiology* / Q000628:therapy / Q000821:virology; D006736:Horses; D058507:Host Specificity; D017900:Myoviridae / Q000145:classification / Q000302:isolation & purification; D012722:Sewage / Q000382:microbiology
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D012722:Sewage; C064517:gp23 protein, Bacteriophage T4
keywords: Biocontrol; Electron micrography; Myoviridae; gp23 gene