title: Features of the Antitumor Effect of Vaccinia Virus Lister Strain.
abstract: Oncolytic abilities of vaccinia virus (VACV) served as a basis for the development of various recombinants for treating cancer; however, "natural" oncolytic properties of the virus are not examined in detail. Our study was conducted to know how the genetically unmodified L-IVP strain of VACV produces its antitumor effect. Human A431 carcinoma xenografts in nude mice and murine Ehrlich carcinoma in C57Bl mice were used as targets for VACV, which was injected intratumorally. A set of virological methods, immunohistochemistry, light and electron microscopy was used in the study. We found that in mice bearing A431 carcinoma, the L-IVP strain was observed in visceral organs within two weeks, but rapidly disappeared from the blood. The L-IVP strain caused decrease of sizes in both tumors, however, in different ways. Direct cell destruction by replicating virus plays a main role in regression of A431 carcinoma xenografts, while in Ehrlich carcinoma, which poorly supported VACV replication, the virus induced decrease of mitoses by pushing tumor cells into S-phase of cell cycle. Our study showed that genetically unmodified VACV possesses at least two mechanisms of antitumor effect: direct destruction of tumor cells and suppression of mitoses in tumor cells.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8 Lavrentiev Avenue, Novosibirsk 630090, Russia. zoman89@gmail.com.
authors.forename: Evgeniy
authors.initials: E
authors.lastname: Zonov
authors.affiliation: State Research Center of Virology and Biotechnology "Vector", Koltsovo 630559, Russia. g.v.kochneva@yandex.ru.
authors.forename: Galina
authors.initials: G
authors.lastname: Kochneva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8 Lavrentiev Avenue, Novosibirsk 630090, Russia. pretty_lier@mail.ru.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Yunusova
authors.affiliation: State Research Center of Virology and Biotechnology "Vector", Koltsovo 630559, Russia. gaa@vector.nsc.ru.
authors.forename: Antonina
authors.initials: A
authors.lastname: Grazhdantseva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8 Lavrentiev Avenue, Novosibirsk 630090, Russia. vrichter@ngs.ru.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Richter
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8 Lavrentiev Avenue, Novosibirsk 630090, Russia. lenryab@yandex.ru.
authors.forename: Elena
authors.initials: E
authors.lastname: Ryabchikova
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics / Q000502:physiology*; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
keywords: VACV-induced arrest of mitoses; genetically unmodified L-IVP strain; human A-431 carcinoma xenografts; murine Ehrlich carcinoma; vaccinia virus