title: Protein-templated cobaltous phosphate nanocomposites for the highly sensitive and selective detection of platelet-derived growth factor-BB.
abstract: We synthesized novel Co3(PO4)2-based nanocomposites with 3D porous architectures via self-assembly; here, bovine serum albumin (BSA) and aptamer were used as organic phases to produce Co3(PO4)2@BSA and Co3(PO4)2@Apt nanocomposites, respectively. The formation mechanism of Co3(PO4)2-based nanocomposites was described based on characterizations of their physio-chemical performance, and the developed nanocomposites were applied as scaffold materials to construct a novel electrochemical aptasensor and detect platelet-derived growth factor-BB (PDGF-BB). The PDGF-BB targeting aptamer must be immobilized onto the Co3(PO4)2@BSA-modified electrode to detect PDGF-BB, whereas Co3(PO4)2@Apt-based aptasensor may be directly used to determine the target protein. Electrochemical impedance spectroscopy results showed that the developed Co3(PO4)2@BSA- and Co3(PO4)2@Apt-based aptasensors present highly sensitive detection ability toward PDGF-BB. Due to the special nanoflower structure, the Co3(PO4)2@BSA-based aptasensor features a detection limit of 3.7 pg mL(-1); while the limit of detection of the Co3(PO4)2@Apt-based aptasensor is 61.5 pg mL(-1), which is the possible bioactivity loss of the aptamer in Co3(PO4)2@Apt nanocomposite. The two detection limits obtained are still much lower than or comparable with those of previously reported aptasensors. The Co3(PO4)2@BSA- and Co3(PO4)2@Apt-based aptasensors showed high selectivity, stability, and applicability for detecting the desired protein. This finding indicates that the Co3(PO4)2-based nanocomposites could be used as an electrochemical biosensor for various detection procedures in the biomedical field.
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Linghao
authors.initials: L
authors.lastname: He
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Ji
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration,Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Peng
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Fufeng
authors.initials: F
authors.lastname: Yan
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China; Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration,Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Fang
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration,Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Hongzhong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China; Henan Collaborative Innovation Center of Food Production and Safety, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Jia
authors.affiliation: State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China; Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration,Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China. Electronic address: mainzhh@163.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D010710:Phosphates; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D012710:Serum Albumin, Bovine; D003035:Cobalt
keywords: Aptasensor; Cobaltous phosphate nanocomposites; Platelet-derived growth factor-BB