title: A highly selective and sensitive near-infrared fluorescent probe for imaging of hydrogen sulphide in living cells and mice.
abstract: Hydrogen sulphide (H2S), the third endogenous gaseous signalling molecule, has attracted attention in biochemical research. The selective detection of H2S in living systems is essential for studying its functions. Fluorescence detection methods have become useful tools to explore the physiological roles of H2S because of their real-time and non-destructive characteristics. Herein we report a near-infrared fluorescent probe, NIR-HS, capable of tracking H2S in living organisms. With high sensitivity, good selectivity and low cytotoxicity, NIR-HS was able to recognize both the exogenous and endogenous H2S in living cells. More importantly, it realized the visualization of endogenous H2S generated in cells overexpressing cystathionine β-synthase (CBS), one of the enzymes responsible for producing endogenous H2S. The probe was also successfully applied to detect both the exogenous and endogenous H2S in living mice. The superior sensing properties of the probe render it a valuable research tool in the H2S-related medical research.
authors.affiliation: School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.|Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210029, China.
authors.forename: Xi Emily
authors.initials: XE
authors.lastname: Zheng
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zou
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Yanguo
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Meng
authors.affiliation: School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
authors.forename: En
authors.initials: E
authors.lastname: Lai
authors.affiliation: School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
authors.forename: Zhichen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.|Guangdong Key Lab of Nano-Micro Material Research, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, 518055, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism; D004136:Dinitrobenzenes / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D012680:Sensitivity and Specificity; D014966:Xanthenes / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004136:Dinitrobenzenes; D005456:Fluorescent Dyes; D015335:Molecular Probes; C000612386:NIR-HS compound; D014966:Xanthenes; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide