title: Validated LC-MS-MS Method for Multiresidual Analysis of 13 Illicit Phenethylamines in Amniotic Fluid.
abstract: A multi-residue analytical method was developed for the determination in amniotic fluid (AF) of 13 illicit phenethylamines, including 12 compounds never investigated in this matrix before. Samples were subject to solid-phase extraction using; hydrophilic-lipophilic balance cartridges which gave good recoveries and low matrix effects on analysis of the extracts. The quantification was performed by liquid chromatography electrospray tandem mass spectrometry. The water-acetonitrile mobile phase containing 0.1% formic acid, used with a C18 reversed phase column, provided adequate separation, resolution and signal-to-noise ratio for the analytes and the internal standard. The final optimized method was validated according to international guidelines. A monitoring campaign to assess fetal exposure to these 13 substances of abuse has been performed on AF test samples obtained from pregnant women. All mothers (n = 194) reported no use of drugs of abuse during pregnancy, and this was confirmed by the analytical data.
authors.affiliation: Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23/a, 07100 Sassari, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Burrai
authors.affiliation: Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23/a, 07100 Sassari, Italy marvi@uniss.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Nieddu
authors.affiliation: Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23/a, 07100 Sassari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Carta
authors.affiliation: Dipartimento di Scienze Biomediche, Università di Sassari, V.le San Pietro 43, 07100 Sassari, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Trignano
authors.affiliation: Dipartimento di Scienze Biomediche, Università di Sassari, V.le San Pietro 43, 07100 Sassari, Italy.
authors.forename: Raimonda
authors.initials: R
authors.lastname: Sanna
authors.affiliation: Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23/a, 07100 Sassari, Italy.
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Boatto
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D010627:Phenethylamines / Q000032:analysis*; D011247:Pregnancy; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013287:Illicit Drugs; D010627:Phenethylamines