title: Enhanced methane production from microalgal biomass by anaerobic bio-pretreatment.
abstract: Anaerobic digestion (AD) of microalgal biomass is one of the most energy efficient technologies to convert microalgae to biofuels. In order to improve the biogas productivity, breaking up the tough and rigid cell wall of microalgae by pretreatment is necessary. In this work, Bacillus licheniformis, a facultative anaerobic bacterial with hydrolytic and acidogenic activities, was adopted to pretreat Chlorella sp. In the established pretreatment process, pure bacterial culture (0%, 1%, 2%, 4%, 8%, v/v) were used to pretreat Chlorella sp. under anaerobic condition at 37°C for 60 h. The soluble chemical oxygen demands (SCOD) content was increased by 16.4-43.4%, while volatile fatty acids (VFAs) were improved by 17.3-44.2%. Furthermore, enhancement of methane production (9.2-22.7%) was also observed in subsequent AD. The results indicated that the more dosages of bacteria were used to pretreat the microalgal biomass in the range of 1-8%, the more methane was produced.
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: He
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Fan
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Naveen Reddy
authors.initials: NR
authors.lastname: Katukuri
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China.
authors.forename: Xianzheng
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China. Electronic address: guorb@qibebt.ac.cn.
authors.forename: Rong-Bo
authors.initials: RB
authors.lastname: Guo
mesh_terms: D000693:Anaerobiosis; D000069976:Bacillus licheniformis / Q000378:metabolism; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors; D000460:Chlorophyta / Q000378:metabolism*; D005232:Fatty Acids, Volatile; D006868:Hydrolysis; D008697:Methane / Q000378:metabolism*; D058086:Microalgae / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D008697:Methane
keywords: Anaerobic digestion; Anaerobic pretreatment; Bacillus licheniformis; Chlorella sp; Microalgae