title: Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey.
abstract: The aim of this study was to investigate Mycoplasma spp. species in blood samples of the domestic cats from the province of Istanbul, Turkey. Three hundred eighty four blood samples of client-owned cats were used for the identification of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMt) by Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays. Out of 384 blood samples, 74 (19.3%) were positive for one of Mycoplasma species. The total prevalence of Mhf, CMhm and CMt infections was 9.9%, 17.7% and 0.8% respectively. The most common species was CMhm. Co-infections were mostly with Mhf/CMhm and the frequency was 8.1%. Two cats were infected with three species. The current study was the first molecular prevalence study of hemotropic mycoplasmas in Istanbul, reporting the presence of CMt for the first time in Turkey. Prevalence of feline mycoplasma was notably high in Istanbul and PCR assay could be preferred rather than the microscopic examination for the diagnosis.
authors.forename: Handan
authors.initials: H
authors.lastname: Cetinkaya
authors.forename: Damla
authors.initials: D
authors.lastname: Haktanir
authors.forename: Seckin
authors.initials: S
authors.lastname: Arun
authors.forename: Cem
authors.initials: C
authors.lastname: Vurusaner
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D001769:Blood / Q000382:microbiology*; D002371:Cat Diseases / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D002415:Cats; D060085:Coinfection / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D014421:Turkey / Q000453:epidemiology; D014730:Veterinary Medicine / Q000379:methods