title: Optimal sample volumes of human trabecular bone in μCT analysis within vertebral body and femoral head.
abstract: Trabecular bones of different skeletal sites have different bone morphologies. How to select an appropriate volume of region of interest (ROI) to reflect the microarchitecture of trabecular bone in different skeletal sites was an interesting problem. Therefore, in this study, the optimal volumes of ROI within vertebral body and femoral head, and if the relationships between volumes of ROI and microarchitectural parameters were affected by trabecular bone morphology were studied. Within vertebral body and femoral head, different cubic volumes of ROI (from (1 mm)(3) to (20 mm)(3)) were set to compare with control groups(whole volume of trabecular bone). Five microarchitectural parameters (BV/TV, Tb.N, Tb.Th, Tb.Sp, and BS/BV) were obtained. Nonlinear curve fitting functions were used to explore the relationships between the microarchitectural parameters and the volumes of ROI. The volumes of ROI could affect the microarchitectural parameters when the volume was smaller than (8 mm)(3) within the vertebral body and smaller than (13 mm)(3) within the femoral head. As the volume increased, the variable tendencies of BV/TV, Tb.N, and Tb.Sp were different between these two skeletal sites. The curve fitting functions between these two sites were also different. The relationships between volumes of ROI and microarchitectural parameters were affected by the different trabecular bone morphologies within lumbar vertebral body and femoral head. When depicting the microarchitecture of human trabecular bone within lumbar vertebral body and femoral head, the volume of ROI would be larger than (8 mm)(3) and (13 mm)(3).
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Wen
authors.affiliation: Department of Oral and Maxillofacial Surgery, State Key Laboratory of Military Stomatology, School of Stomatology, Fourth Military Medical University Xi'an, China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Zong
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, 463 Hospital of PLA Shenyang, China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Fa-Qi
authors.initials: FQ
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Feng
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Ya-Bo
authors.initials: YB
authors.lastname: Yan
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
keywords: human; microarchitecture; region of interest; trabecular bone; μCT