title: The protective effect of daidzein on high glucose-induced oxidative stress in human umbilical vein endothelial cells.
abstract: Endothelial cell dysfunction is considered a major cause of vascular complications in diabetes. In the present study, we investigated the protective effect of daidzein, a natural isoflavonoid, against high-glucose-induced oxidative damage in human umbilical vein endothelial cells (HUVECs). Treatment with a high concentration of glucose (30 mM) induced oxidative stress in the endothelial cells, against which daidzein protected the cells as demonstrated by significantly increased cell viability. In addition, lipid peroxidation, intracellular reactive oxygen species (ROS) generation, and indirect nitric oxide levels induced by the high glucose treatment were significantly reduced in the presence of daidzein (0.02-0.1 mM) in a dose-dependent manner. High glucose levels induced the overexpression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-κB proteins in HUVECs, which was suppressed by treatment with 0.04 mM daidzein. These findings indicate the potential of daidzein to reduce high glucose-induced oxidative stress.
authors.forename: Mi Hwa
authors.initials: MH
authors.lastname: Park
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Ju
authors.forename: Mihyang
authors.initials: M
authors.lastname: Kim
authors.forename: Ji-Sook
authors.initials: JS
authors.lastname: Han
mesh_terms: D002470:Cell Survival / Q000187:drug effects* / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D005947:Glucose / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D016328:NF-kappa B / Q000096:biosynthesis; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D007529:Isoflavones; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C004742:daidzein; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D005947:Glucose