title: Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy.
abstract: The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life.
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 862, Ed. Ciências Biomédicas, 5°andar. Vila Clementino, CEP: 04023-900 São Paulo, SP, Brazil.
authors.forename: Glauber Menezes
authors.initials: GM
authors.lastname: Lopim
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 862, Ed. Ciências Biomédicas, 5°andar. Vila Clementino, CEP: 04023-900 São Paulo, SP, Brazil.
authors.forename: Diego
authors.initials: D
authors.lastname: Vannucci Campos
authors.affiliation: Instituto do Cérebro (INCE), Hospital Israelita Albert Einstein, São Paulo, SP, Brazil; Universidade de Mogi das Cruzes, SP, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Gomes da Silva
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 862, Ed. Ciências Biomédicas, 5°andar. Vila Clementino, CEP: 04023-900 São Paulo, SP, Brazil.
authors.forename: Alexandre Aparecido
authors.initials: AA
authors.lastname: de Almeida
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Lent
authors.affiliation: Departamento de Neurologia e Neurocirurgia, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
authors.forename: Esper Abrão
authors.initials: EA
authors.lastname: Cavalheiro
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 862, Ed. Ciências Biomédicas, 5°andar. Vila Clementino, CEP: 04023-900 São Paulo, SP, Brazil. Electronic address: arida.nexp@epm.br.
authors.forename: Ricardo Mario
authors.initials: RM
authors.lastname: Arida
mesh_terms: D000818:Animals; D002452:Cell Count; D006624:Hippocampus / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D009474:Neurons / Q000473:pathology* / Q000502:physiology*; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D010862:Pilocarpine
keywords: Epilepsy; Hippocampus; Neuronal cell; Non-neuronal cell; Seizure frequency