title: Altered lipid accumulation inNannochloropsis salinaCCAP849/3 following EMS and UV induced mutagenesis.
abstract: Microalgae have potential as a chemical feed stock in a range of industrial applications.Nannochloropsis salinawas subject to EMS mutagenesis and the highest lipid containing cells selected using fluorescence-activated cell sorting. Assessment of growth, lipid content and fatty acid composition identified mutant strains displaying a range of altered traits including changes in the PUFA content and a total FAME increase of up to 156% that of the wild type strain. Combined with a reduction in growth this demonstrated a productivity increase of up to 76%. Following UV mutagenesis, lipid accumulation of the mutant cultures was elevated to more than 3 fold that of the wild type strain, however reduced growth rates resulted in a reduction in overall productivity. Changes observed are indicative of alterations to the regulation of the omega 6 Kennedy pathway. The importance of these variations in physiology for industrial applications such as biofuel production is discussed.
authors.affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, Devon PL1 3DH, UK.
authors.forename: T A
authors.initials: TA
authors.lastname: Beacham
authors.affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, Devon PL1 3DH, UK; Plymouth University, Drake Circus, Plymouth, PL4 8AA, UK.
authors.forename: V Mora
authors.initials: VM
authors.lastname: Macia
authors.affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, Devon PL1 3DH, UK.
authors.forename: P
authors.initials: P
authors.lastname: Rooks
authors.affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, Devon PL1 3DH, UK; Plymouth University, Drake Circus, Plymouth, PL4 8AA, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: White
authors.affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, Devon PL1 3DH, UK.
authors.forename: S T
authors.initials: ST
authors.lastname: Ali
keywords: Biofuels; Lipid; Microalgae; Nannochloropsis; Random mutation; UV