title: The Protective Effects of Alisol A 24-Acetate from Alisma canaliculatum on Ovariectomy Induced Bone Loss in Vivo.
abstract: Alisma canaliculatum is a herb commonly used in traditional Korean medicine, and has been shown in scientific studies to have antitumor, diuretic hepatoprotective, and antibacterial effects. Recently, the anti-osteoclastogenesis of alisol A 24-acetate from Alisma canaliculatum was investigated in vitro. However, the influence of alisol A 24-acetate on osteoporosis in animals has not been investigated. The present study was undertaken to investigate the anti-osteoporotic effect of alisol A 24-acetate on bone mass in ovariectomized (OVX) mice and to identify the mechanism responsible for its effects. OVX mice were treated daily with 0.5 or 2 μg/g of alisol A 24-acetate for a period of six weeks. It was found that these administrations significantly suppressed osteoporosis in OVX mice and improved bone morphometric parameters. The serum estradiol, bone alkaline phosphatase levels, regulatory T/Th17 cell numbers were significantly increased by alisol A 24-acetate as compared with untreated OVX mice. In addition, TRAP activity was inhibited by alisol A 24-acetate in OVX mice. These results suggest alisol A 24-acetate effectively prevents bone loss in OVX mice, and that it can be considered a potential therapeutic for the treatment of postmenopausal osteoporosis.
authors.affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea. hyh7733@naver.com.
authors.forename: Yun-Ho
authors.initials: YH
authors.lastname: Hwang
authors.affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea. kang8404@nate.com.
authors.forename: Kyung-Yun
authors.initials: KY
authors.lastname: Kang
authors.affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea. 6525140@hanmail.net.
authors.forename: Sung-Ju
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea. sjnam@ewha.ac.kr.
authors.forename: Sang-Jip
authors.initials: SJ
authors.lastname: Nam
authors.affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea. sony@sunchon.ac.kr.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Son
authors.affiliation: Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea. sungtae@sunchon.ac.kr.
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Yee
mesh_terms: D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D034902:Alisma / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001862:Bone Resorption / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002783:Cholestenones / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000097:blood; D018655:Lymphocyte Count; D051379:Mice; D008809:Mice, Inbred C3H; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy / Q000009:adverse effects; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects; D000071681:Tartrate-Resistant Acid Phosphatase; D058504:Th17 Cells / Q000187:drug effects
chemical_list: D050071:Bone Density Conservation Agents; D002783:Cholestenones; D007527:Isoenzymes; D010936:Plant Extracts; C457233:alisol A 24-acetate; D004958:Estradiol; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Alisma canaliculatum; alisol A 24-acetate; bone loss; osteoporosis; regulator T cell; traditional Korean medicine