title: Infectious Spondylitis in a Patient with Chronic Kidney Disease: Identification of Campylobacter fetus Subsp. testudinum by 16S Ribosomal RNA Sequencing.
abstract: We report the first case of spondylitis with bacteremia caused by Campylobacter fetus subsp. testudinum identified by 16S ribosomal ribonucleic acid (rRNA) gene sequencing. An 81-year-old man presented with fever and general weakness. His medical history included end-stage renal disease, hypertension, and type 2 diabetes. Despite empirical antibiotic treatment, his fever and back pain persisted. Magnetic resonance imaging with gadolinium enhancement showed a low-signal-intensity lesion in T1-weighted imaging and a high-signal-intensity lesion in T2-weighted imaging at the L3 vertebral body. C. fetus grew on 1 pair of blood cultures. C. fetus subsp. testudinum was identified via 16S rRNA sequencing of the cultivated organisms. The patient recovered uneventfully after 6 weeks of optimal antibiotic treatment, selected using susceptibility tests. C. fetus spondylitis is a very rare disease. In this unique case involving end-stage renal disease, the underlying pathogen was identified by 16S rRNA sequencing.
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School.
authors.forename: Hong Sang
authors.initials: HS
authors.lastname: Choi
authors.forename: Sung Un
authors.initials: SU
authors.lastname: Shin
authors.forename: Eun Hui
authors.initials: EH
authors.lastname: Bae
authors.forename: Seong Kwon
authors.initials: SK
authors.lastname: Ma
authors.forename: Soo Wan
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D001431:Bacteriological Techniques; D002169:Campylobacter Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D002168:Campylobacter fetus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging; D008279:Magnetic Resonance Imaging; D008297:Male; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D051436:Renal Insufficiency, Chronic / Q000150:complications*; D017422:Sequence Analysis, DNA; D013166:Spondylitis / Q000175:diagnosis* / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S