title: Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
abstract: D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability. The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design. The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3. These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.
authors.affiliation: Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Wei
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: WenJing
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: FengJie
authors.initials: F
authors.lastname: Cui
authors.affiliation: Shandong Depu Chemical Technology Co., Ltd., Tai'an 271200, China.
authors.forename: QinHua
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Shandong Depu Chemical Technology Co., Ltd., Tai'an 271200, China.
authors.forename: ChangFeng
authors.initials: C
authors.lastname: Liu
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D008962:Models, Theoretical; D012266:Ribose / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D013213:Starch / Q000737:chemistry; D014174:Transketolase / Q000235:genetics*; D003313:Zea mays / Q000737:chemistry
chemical_list: D003470:Culture Media; D012266:Ribose; D013213:Starch; D014174:Transketolase; D005947:Glucose