title: Essential Contribution of CD4+ T Cells to Antigen-Induced Nasal Hyperresponsiveness in Experimental Allergic Rhinitis.
abstract: Nasal hyperresponsiveness (NHR) is a characteristic feature of allergic rhinitis (AR); however, the pathogenesis of NHR is not fully understood. In this study, during the establishment of an experimental AR model using ovalbumin-immunized and -challenged mice, augmentation of the sneezing reaction in response to nonspecific proteins as well as a chemical stimulant was detected. Whether NHR is independent of mast cells and eosinophils was determined by using mast cell- and eosinophil-deficient mice. NHR was suppressed by treatment with anti-CD4 antibody, suggesting the pivotal contribution of CD4+ T cells. Furthermore, antigen challenge to mice to which in vitro-differentiated Th1, Th2, and Th17 cells but not naïve CD4+ T cells had been adoptively transferred led to the development of equivalent NHR. Since antigen-specific IgE and IgG were not produced in these mice and since antigen-specific IgE-transgenic mice did not develop NHR even upon antigen challenge, humoral immunity would be dispensable for NHR. CD4+ T cells play a crucial role in the pathogenesis of AR via induction of NHR, independent of IgE-, mast cell-, and eosinophil-mediated responses.
authors.affiliation: Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Tomoe
authors.initials: T
authors.lastname: Nishimura
authors.affiliation: Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
authors.affiliation: Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Saeki
authors.affiliation: Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitamura
authors.affiliation: Mammalian Genetics Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Kunie
authors.initials: K
authors.lastname: Matsuoka
authors.affiliation: Basic Research Center, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yonekawa
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Kanagawa, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.affiliation: Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects; D008807:Mice, Inbred BALB C; D009666:Nose / Q000473:pathology*; D012130:Respiratory Hypersensitivity / Q000150:complications* / Q000276:immunology*; D065631:Rhinitis, Allergic / Q000150:complications* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D007073:Immunoglobulin E