title: Interactions between N-Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus.
abstract: Glucocorticoid hormones, via activation of their receptors, promote memory consolidation, but the exact underlying mechanisms remain elusive. We examined how corticosterone regulates AMPA receptor (AMPAR) availability in the synapse, which is important for synaptic plasticity and memory formation. Peptides which specifically block the interaction between N-Ethylmaleimide-Sensitive Factor (NSF) and the AMPAR-subunit GluA2 prevented the increase in synaptic transmission and surface expression of AMPARs known to occur after corticosterone application to hippocampal neurons. Combining a live imaging Fluorescence Recovery After Photobleaching (FRAP) approach with the use of the pH-sensitive GFP-AMPAR tagging revealed that this NSF/GluA2 interaction was also essential for the increase of the mobile fraction and reduction of the diffusion of AMPARs after treating hippocampal neurons with corticosterone. We conclude that the interaction between NSF and GluA2 contributes to the effects of corticosterone on AMPAR function. © 2016 Wiley Periodicals, Inc.
authors.affiliation: SILS-CNS, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Centre National De La Recherche Scientifique, University of Nice - Sophia-Antipolis Institut De Pharmacologie Moléculaire Et Cellulaire, UMR7275, Valbonne, 06560, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Cassé
authors.affiliation: Institute of Neuroscience, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 20031, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: SILS-CNS, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Xiong
authors.affiliation: SILS-CNS, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.
authors.forename: Marian
authors.initials: M
authors.lastname: Joels
authors.affiliation: Centre National De La Recherche Scientifique, University of Nice - Sophia-Antipolis Institut De Pharmacologie Moléculaire Et Cellulaire, UMR7275, Valbonne, 06560, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Martin
authors.affiliation: SILS-CNS, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.
authors.forename: Harm J
authors.initials: HJ
authors.lastname: Krugers
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D005938:Glucocorticoids / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D005938:Glucocorticoids; D018091:Receptors, AMPA; D052066:N-Ethylmaleimide-Sensitive Proteins; C498737:Nsf protein, rat; D003345:Corticosterone
keywords: AMPA receptor; AP2; NSF; stress; synapses