title: Clinical and immunological responses of dust mite sensitive, atopic dogs to treatment with sublingual immunotherapy (SLIT).
abstract: BACKGROUND
  Sublingual immunotherapy (SLIT) has been reported to be beneficial in people with atopic dermatitis (AD) and dust mite sensitivity. Evaluation of this therapy has not been reported in spontaneous canine AD.
  
  
  OBJECTIVE
  The objective of this study was to preliminarily evaluate the effectiveness of an established SLIT protocol, as used in human patients, in dogs with AD.
  
  
  METHODS
  Ten dust mite sensitive dogs with spontaneous AD.
  
  
  METHODS
  Dogs underwent a 6 month open trial of SLIT concurrently with decreasing dose oral methylprednisolone. Clinical evaluations and quantitative serum anti-mite IgE and IgG levels were performed every 2 months.
  
  
  RESULTS
  Mean methylprednisolone use from the first 2 months of the study to the final 2 months declined from 10.2 to 4.3 mg/kg/2 months (P < 0.001, Student's paired t-test); at 6 months, four dogs required no oral corticosteroid administration. Over the course of the study, median Canine Atopic Dermatitis Extent and Severity Index (CADESI)-03 scores declined from 76.5 to 59; median pruritus scores declined from 65 to 37 (P < 0.02 and P < 0.01, respectively; Wilcoxon signed-rank test). Pre- and post-SLIT intradermal test scores for mite allergen were not significantly different over time. Median Dermatophagoides farinae (DF)-specific IgE levels declined significantly from 150.2 × 10(3) AU/mL to 3.6 × 10(3) AU/mL (P < 0.05). Concurrently, median DF-specific IgG levels increased from 18.5 × 10(6) AU/mL to 3923.4 × 10(6) AU/mL (P < 0.05; Wilcoxon signed-rank tests).
  
  
  CONCLUSIONS
  SLIT treatment produced clinical improvement in dogs with dust mite-associated AD and was associated with serological changes supporting this improvement. Further studies in larger numbers of dogs and those with polysensitization are warranted.
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive, Madison, WI, 53706, USA.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: DeBoer
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive, Madison, WI, 53706, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Verbrugge
authors.affiliation: Allergy Associates of La Crosse, 2727 Midwest Drive, Onalaska, WI, 54650, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Morris
mesh_terms: D000286:Administration, Sublingual; D000818:Animals; D003876:Dermatitis, Atopic / Q000628:therapy / Q000662:veterinary*; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D007167:Immunotherapy / Q000379:methods / Q000662:veterinary*; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use; D039981:Pyroglyphidae / Q000276:immunology*
chemical_list: D005938:Glucocorticoids; D008775:Methylprednisolone