title: Effect of Periodic Granulocyte Colony-Stimulating Factor Administration on Endothelial Progenitor Cells and Different Monocyte Subsets in Pediatric Patients with Muscular Dystrophies.
abstract: Muscular dystrophies (MD) are heterogeneous group of diseases characterized by progressive muscle dysfunction. There is a large body of evidence indicating that angiogenesis is impaired in muscles of MD patients. Therefore, induction of dystrophic muscle revascularization should become a novel approach aimed at diminishing the extent of myocyte damage. Recently, we and others demonstrated that administration of granulocyte colony-stimulating factor (G-CSF) resulted in clinical improvement of patients with neuromuscular disorders. To date, however, the exact mechanisms underlying these beneficial effects of G-CSF have not been fully understood. Here we used flow cytometry to quantitate numbers of CD34+ cells, endothelial progenitor cells, and different monocyte subsets in peripheral blood of pediatric MD patients treated with repetitive courses of G-CSF administration. We showed that repetitive cycles of G-CSF administration induced efficient mobilization of above-mentioned cells including cells with proangiogenic potential. These findings contribute to better understanding the beneficial clinical effects of G-CSF in pediatric MD patients.
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland.
authors.forename: Andrzej
authors.identifier: 0000-0002-8980-1474
authors.initials: A
authors.lastname: Eljaszewicz
authors.affiliation: Department of Pediatric Rehabilitation and Center of Early Support for Handicapped Children "Give a Chance", Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Sienkiewicz
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland; Department of Immunology, Medical University of Bialystok, 15-269 Bialystok, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Grubczak
authors.affiliation: Department of Pediatric Rehabilitation and Center of Early Support for Handicapped Children "Give a Chance", Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Okurowska-Zawada
authors.affiliation: Department of Pediatric Rehabilitation and Center of Early Support for Handicapped Children "Give a Chance", Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Paszko-Patej
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland.
authors.forename: Paula
authors.initials: P
authors.lastname: Miklasz
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Radzikowska
authors.affiliation: Department of Pediatric Rehabilitation and Center of Early Support for Handicapped Children "Give a Chance", Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kulak
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland; Department of Allergology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Marcin
authors.identifier: 0000-0001-7183-3120
authors.initials: M
authors.lastname: Moniuszko