title: Ischemic preconditioning and inflammatory response syndrome after reperfusion injury: an experimental model in diabetic rats.
abstract: Quantification of local ischemia and inflammatory response syndrome correlated with histological changes associated with ischemia-reperfusion injury (IRI) after revascularization techniques. We included 12 adult male Wistar rats, aged eight weeks that were randomly divided into two groups. The first group acted as the control and at the second group, we induced diabetes by intraperitoneal streptozotocin administration (60 mg/kg). After eight weeks, the rats were subject to ischemic preconditioning for 10 minutes at three regular intervals. Twenty-four hours post-preconditioning, both groups were subject to ischemia for 20 minutes, followed by 30 minutes of reperfusion. Oxygen extraction was higher in Group 1, the arterio-venous CO2 gradient was higher in the control group, but not significant. The lactate production was higher in Group 1. The second group had a higher Na+ and also a significant difference in K+ values. Receptor for Advanced Glycation End (RAGE) values were higher in the second group but with no significant difference (RAGE1=0.32 ng/mL versus RAGE2=0.40 ng/mL). The muscle samples from the control group displayed significant rhabdomyolysis, damage to the nucleus, while the preconditioned group showed almost normal morphological characteristics. The lungs and kidneys were most damaged in the control group, with damage expressed as thickened alveolar septa, neutrophil infiltrates, eosinophilic precipitates in the proximal convolute tubule. Ischemic preconditioning significantly attenuates the ischemic reperfusion injury.
authors.affiliation: Department of Infectious Diseases, University of Medicine and Pharmacy of Tirgu Mures, Romania; ancameda.georgescu@umftgm.ro.
authors.forename: Bianca Liana
authors.initials: BL
authors.lastname: Grigorescu
authors.forename: Anca Meda
authors.initials: AM
authors.lastname: Georgescu
authors.forename: Adrian-Dan
authors.initials: AD
authors.lastname: Cioc
authors.forename: Raluca-Ştefania
authors.initials: RŞ
authors.lastname: Fodor
authors.forename: Ovidiu Simion
authors.initials: OS
authors.lastname: Cotoi
authors.forename: Pal
authors.initials: P
authors.lastname: Fodor
authors.forename: Sanda Maria
authors.initials: SM
authors.lastname: Copotoiu
authors.forename: Leonard
authors.initials: L
authors.lastname: Azamfirei
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000473:pathology; D004195:Disease Models, Animal; D000860:Hypoxia / Q000150:complications; D007249:Inflammation / Q000150:complications* / Q000473:pathology*; D007477:Ions; D019194:Ischemic Preconditioning; D008297:Male; D009928:Organ Specificity; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism; D015427:Reperfusion Injury / Q000150:complications* / Q000473:pathology; D013577:Syndrome
chemical_list: C000593874:Ager protein, rat; D007477:Ions; D000067759:Receptor for Advanced Glycation End Products