title: A Microfluidic Approach to Investigating a Synergistic Effect of Tobramycin and Sodium Dodecyl Sulfate on Pseudomonas aeruginosa Biofilms.
abstract: In recent years, a microfluidic technology has contributed a significant role in biological research, specifically for the study of biofilms. Bacterial biofilms are a source of infections and contamination in the environment due to an extra polymeric matrix. Inadequate uses of antibiotics make the bacterial biofilms antibiotic resistant. Therefore, it is important to determine the effective concentration of antibiotics in order to eliminate bacterial biofilms. The present microfluidic study was carried out to analyze the activities of tobramycin and sodium dodecyl sulfate (SDS) against Pseudomonas aeruginosa biofilms with a continuous flow in order to achieve a greater delivery of the agents. The results show that a co-treatment of tobramycin and SDS significantly reduced the biomass of biofilms (by more than 99%) after 24 h. Tobramycin and SDS killed and detached bacteria in the cores of biofilms. Evidently, our data suggest that a microchannel would be effective for both quantitative and qualitative evaluations in order to test combinatorial effect of drugs and chemicals on a complexed biological system including biofilm.
authors.affiliation: Department of Bionano Engineering, Hanyang University.
authors.forename: Soojeong
authors.initials: S
authors.lastname: Shin
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
authors.forename: Jangsun
authors.initials: J
authors.lastname: Hwang
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Seo
authors.forename: Eunwon
authors.initials: E
authors.lastname: Lee
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Choi
authors.forename: Sangjun
authors.initials: S
authors.lastname: Moon
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Hong
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005898:Glass / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000494:pharmacology*; D014031:Tobramycin / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012967:Sodium Dodecyl Sulfate; D014031:Tobramycin