title: Effects of Water-Soluble Polysaccharides with Different Chemical Structure, Isolated from Acorus calamus L. and Trifolium pratense L., on Nitric Oxide Production: A Screening Study.
abstract: Screening study of the effects of sweet flag (Acorus calamus L.) rhizome and clover (Trifolium pratense L.) aerial part on the production of NO by mouse macrophages was carried out. The polysaccharides were separated by ion exchange chromatography into fractions differing by monomeric composition and ramification type and were used in concentrations of 20, 40, and 100 μg/ml. Four fractions of Acorus calamus L. (PSF-101, PSF-102, PSF-103, and PSF-105), used in different concentrations, moderately stimulated nitrite production by macrophages. Three of five Trifolium pratense L. polysaccharides (PS62-3, PS62-4, and PS62-5) exhibited a significant specific effect on NO production. Rhamnogalactouronans from clover PS63-3 in all concentrations and from PS62-5 in a concentration of 100 μg/ml exhibited the highest activity, comparable to the NO-stimulatory activity of the reference LPS, while polysaccharide PS62-3 in a concentration of 40 μg/ml exhibited even higher activity.
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Ligacheva
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. m.danilets@mail.ru.
authors.forename: M G
authors.initials: MG
authors.lastname: Danilets
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Trofimova
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Sherstoboev
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Zhdanov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Guriev
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Belousov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.|National Research Tomsk Polytechnological University, Tomsk, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Yusubov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Korzh
authors.affiliation: Siberian State Medical University, Tomsk, Russia.|National Research Tomsk Polytechnological University, Tomsk, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Krivoshchekov
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
mesh_terms: D029023:Acorus / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D010368:Pectins / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D029921:Trifolium / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D009569:Nitric Oxide; D010368:Pectins
keywords: classical activation; macrophages; nitric oxide; pectin polysaccharides; rhamnogalactouronan