title: Fast synthesis and bioconjugation of (68) Ga core-doped extremely small iron oxide nanoparticles for PET/MR imaging.
abstract: Combination of complementary imaging techniques, like hybrid PET/MRI, allows protocols to be developed that exploit the best features of both. In order to get the best of these combinations the use of dual probes is highly desirable. On this sense the combination of biocompatible iron oxide nanoparticles and 68Ga isotope is a powerful development for the new generation of hybrid systems and multimodality approaches. Our objective was the synthesis and application of a chelator-free 68Ga-iron oxide nanotracer with improved stability, radiolabeling yield and in vivo performance in dual PET/MRI. We carried out the core doping of iron oxide nanoparticles, without the use of any chelator, by a microwave-driven protocol. The synthesis allowed the production of extremely small (2.5 nm) 68Ga core-doped iron oxide nanoparticles. The microwave approach allowed an extremely fast synthesis with a 90% radiolabeling yield and T1 contrast in MRI. With the same microwave approach the nano-radiotracer was functionalized in a fast and efficient way. We finally evaluated these dual targeting nanoparticles in an angiogenesis murine model by PET/MR imaging. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.|Universidad Complutense de Madrid and CIBERES, 28040, Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Pellico
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.|Universidad Complutense de Madrid and CIBERES, 28040, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Ruiz-Cabello
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Saiz-Alía
authors.affiliation: Technological Support Center (CAT), Universidad Rey Juan Carlos, Móstoles, Spain.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Del Rosario
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Caja
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Montoya
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Fernández de Manuel
authors.affiliation: Departamento de Biomateriales y Materiales Bioinspirados, Instituto de Ciencia de Materiales de Madrid, CSIC, Madrid, Spain.
authors.forename: M Puerto
authors.initials: MP
authors.lastname: Morales
authors.affiliation: Departamento de Biomateriales y Materiales Bioinspirados, Instituto de Ciencia de Materiales de Madrid, CSIC, Madrid, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Gutiérrez
authors.affiliation: Physics Department, Universidad Carlos III, Av de la Universidad 40, 28911, Leganés, Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Galiana
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Enríquez
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Herranz
mesh_terms: D000792:Angiography / Q000379:methods; D000818:Animals; D005290:Ferric Compounds; D005710:Gallium Radioisotopes; D008279:Magnetic Resonance Imaging / Q000379:methods; D053768:Metal Nanoparticles; D051379:Mice; D008872:Microwaves; D064847:Multimodal Imaging / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods
chemical_list: D005290:Ferric Compounds; D005710:Gallium Radioisotopes; C000499:ferric oxide
keywords: Iron Oxide; PET/MRI; T1-MRI; chelator-free 68Ga; nano-radiotracer