title: Functionalization of an Antisense Small RNA.
abstract: In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5' untranslated region that can be activated by this new riboregulator. As a result, both RNAs can orthogonally trans-activate translation of their cognate, independent targets. The two riboregulators can also repress each other by antisense interaction, although not symmetrically. Our work highlights that antisense small RNAs can work as regulatory agents beyond the antisense paradigm and that, hence, they could be interfaced with other circuits used in synthetic biology.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rodrigo
authors.affiliation: School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cordero
authors.affiliation: School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: Manish
authors.initials: M
authors.lastname: Kushwaha
authors.affiliation: School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom; Institute of Systems and Synthetic Biology, Centre National de la Recherche Scientifique, Université d'Evry val d'Essonne, 91000 Évry, France. Electronic address: Alfonso.Jaramillo@warwick.ac.uk.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Jaramillo
mesh_terms: D060846:Cell Engineering / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism*; D058727:RNA, Small Untranslated / Q000235:genetics / Q000378:metabolism*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D016372:RNA, Antisense; D058727:RNA, Small Untranslated
keywords: biotechnology; computational design; information encoding; regulatory RNA; synthetic biology