title: Five New Cytotoxic Metabolites from the Marine Fungus Neosartorya pseudofischeri.
abstract: The marine fungus Neosartorya pseudofischeri was isolated from Acanthaster planci from the South China Sea. In a preliminary bioactivity screening, the crude methanol extract of the fungal mycelia showed significant inhibitory activity against the Sf9 cell line from the fall armyworm Spodoptera frugiperda. Five novel compounds, including 5-olefin phenylpyropene A (1), 13-dehydroxylpyripyropene A (4), deacetylsesquiterpene (7), 5-formyl-6-hydroxy-8-isopropyl-2- naphthoic acid (9) and 6,8-dihydroxy-3-((1E,3E)-penta-1,3-dien-1-yl)isochroman-1-one (10), together with eleven known compounds, phenylpyropene A (2) and C (3), pyripyropene A (5), 7-deacetylpyripyropene A (6), (1S,2R,4aR,5R,8R,8aR)-1,8a-dihydroxy-2-acetoxy-3,8-dimethyl-5- (prop-1-en-2-yl)-1,2,4a, 5,6,7,8,8a-octahydronaphthalene (8), isochaetominine C (11), trichodermamide A (12), indolyl-3-acetic acid methyl ester (13), 1-acetyl-β-carboline (14), 1,2,3,4-tetrahydro-6-hydroxyl-2-methyl-l,3,4-trioxopyrazino[l,2-a]-indole (15) and fumiquinazoline F (16), were obtained. The structures of these compounds were determined mainly by MS and NMR data. The absolute configuration of 9 was assigned by the single-crystal X-ray diffraction studies. Compounds 1-11 and 15 showed significant cytotoxicity against the Sf9 cells from S. frugiperda.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. lanwj@mail.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. lanwj@mail.sysu.edu.cn.
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Lan
authors.affiliation: Lab of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China. marnatprod2015@yeah.net.
authors.forename: Sheng-Jiao
authors.initials: SJ
authors.lastname: Fu
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. xumy3@mail2.sysu.edu.cn.
authors.forename: Meng-Yang
authors.initials: MY
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. natprodlwl@gmail.com.
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Liang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. cklam@mail.sysu.edu.cn.
authors.forename: Chi-Keung
authors.initials: CK
authors.lastname: Lam
authors.affiliation: Lab of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China. guohuazhong@scau.edu.cn.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Zhong
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. junxu@biochemomes.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. lssydp@mail.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. lssydp@mail.sysu.edu.cn.
authors.forename: De-Po
authors.initials: DP
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. ceslhj@mail.sysu.edu.cn.
authors.forename: Hou-Jin
authors.initials: HJ
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D002681:China; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology; D055326:Neosartorya; D012623:Seawater; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D018411:Spodoptera / Q000187:drug effects; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D007306:Insecticides; D012717:Sesquiterpenes
keywords: Neosartorya pseudofischeri; cytotoxic activity; marine fungus; phenylpyropene; pyripyropene; sesquiterpene