title: Lack of effects on key cellular parameters of MRC-5 human lung fibroblasts exposed to 370 mT static magnetic field.
abstract: The last decades have seen increased interest toward possible adverse effects arising from exposure to intense static magnetic fields. This concern is mainly due to the wider and wider applications of such fields in industry and clinical practice; among them, Magnetic Resonance Imaging (MRI) facilities are the main sources of exposure to static magnetic fields for both general public (patients) and workers. In recent investigations, exposures to static magnetic fields have been demonstrated to elicit, in different cell models, both permanent and transient modifications in cellular endpoints critical for the carcinogenesis process. The World Health Organization has therefore recommended in vitro investigations as important research need, to be carried out under strictly controlled exposure conditions. Here we report on the absence of effects on cell viability, reactive oxygen species levels and DNA integrity in MRC-5 human foetal lung fibroblasts exposed to 370 mT magnetic induction level, under different exposure regimens. Exposures have been performed by using an experimental apparatus designed and realized for operating with the static magnetic field generated by permanent magnets, and confined in a magnetic circuit, to allow cell cultures exposure in absence of confounding factors like heating or electric field components.
authors.affiliation: CNR - Institute for Electromagnetic Sensing of Environment, Via Diocleziano 328, 80124 Naples, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Romeo
authors.affiliation: CNR - Institute for Electromagnetic Sensing of Environment, Via Diocleziano 328, 80124 Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Sannino
authors.affiliation: CNR - Institute for Electromagnetic Sensing of Environment, Via Diocleziano 328, 80124 Naples, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Scarfì
authors.affiliation: CNR - Institute for Electromagnetic Sensing of Environment, Via Diocleziano 328, 80124 Naples, Italy.|Department of Physics, University of Naples Federico II, CMSA via Cintia, 80126, Napoli, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Massa
authors.affiliation: Italian Workers' Compensation Authority (INAIL)-Regional Technical Advisory Department Risk and Prevention Assessment (CONTARP) of Campania, via Nuova Poggioreale, 80143 Napoli.
authors.forename: Raffaele
authors.initials: R
authors.lastname: d'Angelo
authors.affiliation: CNR - Institute for Electromagnetic Sensing of Environment, Via Diocleziano 328, 80124 Naples, Italy.
authors.forename: Olga
authors.initials: O
authors.lastname: Zeni
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004574:Electromagnetic Fields / Q000009:adverse effects; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects; D008279:Magnetic Resonance Imaging; D016273:Occupational Exposure; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species