title: Bioactivity of periodontal ligament stem cells on sodium titanate coated with graphene oxide.
abstract: As a biocompatible and low cytotoxic nanomaterial, graphene oxide (GO) has captured tremendous interests in tissue engineering. However, little is known about the behavior of dental stem cells on GO. This study was to evaluate the bioactivity of human periodontal ligament stem cells (PDLSCs) on GO coated titanium (GO-Ti) substrate in vitro as compared to sodium titanate (Na-Ti) substrate. By scanning electron microscope (SEM), confocal laser scanning microscope (CLSM), methylthiazol tetrazolium (MTT) assay, alkaline phosphatase (ALP) activity, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis, we investigated the attachment, morphology, proliferation and osteogenic differentiation of PDLSCs on these two substrates. When seeded on GO-Ti substrate, PDLSCs exhibited significantly higher proliferation rate, ALP activity and up-regulated gene expression level of osteogenesis-related markers of collagen type I (COL-I), ALP, bone sialoprotein (BSP), runt related transcription factor 2 (Runx2) and osteocalcin (OCN) compared with those on Na-Ti substrate. Moreover, GO promoted the protein expression of BSP, Runx2 and OCN. These findings suggest that the combination of GO and PDLSCs provides a promising construct for regenerative dentistry.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Pishan
authors.initials: P
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, China.
authors.forename: Xianlei
authors.initials: X
authors.lastname: Li
authors.affiliation: Center of Bio and Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, Jinan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D005786:Gene Expression Regulation; D006108:Graphite; D006801:Humans; D016130:Immunophenotyping; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D010087:Oxides; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds; D014025:Titanium
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D010087:Oxides; D015675:Osteocalcin; C471701:sodium titanate; D006108:Graphite; D014025:Titanium; D000469:Alkaline Phosphatase