title: The effect of an inhibitor of gut serotonin (LP533401) during the induction of periodontal disease.
abstract: OBJECTIVE
  LP533401 is an inhibitor of tryptophan hydroxylase 1, which regulates serotonin production in the gut. Previous work indicates that LP533401 has an anabolic effect in bone. Thus, we hypothesized that inhibition of gut serotonin production may modulate the host response in periodontal disease. In this study, we aimed to analyze the effects of LP533401 in a rat periodontitis model to evaluate the role of gut serotonin in periodontitis pathophysiology.
  
  
  METHODS
  Twenty-four rats were divided into three groups: treated group (T: ligature-induced periodontal disease and LP533401, 25 mg/kg/d) by gavage; ligature group (L: ligature-induced periodontal disease only); and control group (C: without ligature-induced periodontal disease). After 28 d, radiographic alveolar bone support was measured on digital radiographs, and alveolar bone volume fraction, tissue mineral density and trabeculae characteristics were quantified by microcomputed tomography in the right hemi-mandible. Left hemi-mandibles were decalcified and alveolar bone loss, attachment loss and area of collagen in the gingiva were histologically analyzed.
  
  
  RESULTS
  Significant difference between the L and C groups was found, confirming that periodontal disease was induced. We observed no difference between the T and L groups regarding alveolar bone destruction and area of collagen.
  
  
  CONCLUSIONS
  LP533401 (25 mg/kg/d) for 28 d does not prevent bone loss and does not modulate host response in a rat model of induced periodontal disease.
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista - UNESP, São José dos Campos, SP, Brazil.
authors.forename: G M G
authors.initials: GM
authors.lastname: Lima
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista - UNESP, São José dos Campos, SP, Brazil.
authors.forename: B J M
authors.initials: BJ
authors.lastname: Corazza
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista - UNESP, São José dos Campos, SP, Brazil.
authors.forename: R M
authors.initials: RM
authors.lastname: Moraes
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista - UNESP, São José dos Campos, SP, Brazil.
authors.forename: F E
authors.initials: FE
authors.lastname: de Oliveira
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista - UNESP, São José dos Campos, SP, Brazil.
authors.forename: L D
authors.initials: LD
authors.lastname: de Oliveira
authors.affiliation: Department of General Biology, State University of Ponta Grossa - UEPG, Ponta Grossa, Paraná, Brazil.
authors.forename: G C N
authors.initials: GC
authors.lastname: Franco
authors.affiliation: Vanderbilt Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Orthopedic Surgery & Rehabilitation and Vanderbilt University Institute of Imaging Sciences, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Perrien
authors.affiliation: Vanderbilt Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Departments of Medicine, Pharmacology and Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: F
authors.initials: F
authors.lastname: Elefteriou
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista - UNESP, São José dos Campos, SP, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Anbinder
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D003094:Collagen; D004195:Disease Models, Animal; D005881:Gingiva / Q000473:pathology; D008026:Ligation / Q000009:adverse effects; D008297:Male; D008334:Mandible / Q000473:pathology; D017622:Periodontal Attachment Loss / Q000000981:diagnostic imaging / Q000517:prevention & control; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000473:pathology*; D010518:Periodontitis / Q000188:drug therapy / Q000473:pathology; D011743:Pyrimidines / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism* / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: C548240:LP533401; D011743:Pyrimidines; D012701:Serotonin; D003094:Collagen
keywords: bone; enterochromaffin cells; periodontitis; serotonin