title: Hypoxia alters steroidogenesis in female marine medaka through miRNAs regulation.
abstract: Hypoxia is a worldwide environmental problem in marine ecosystems, leading to serious declines in fishery production over large areas. Our previous studies demonstrated that hypoxia is an endocrine disruptor which can cause reproductive impairment through the regulation of miRNAs, suggesting the functional role of miRNAs in reproductive systems in response to hypoxia. In this study, we used small RNA sequencing to determine the change in miRNA profile in ovary of marine medaka Oryzias melastigma under hypoxic stress. A total of 509 miRNAs were found in the ovary of marine medaka, in which, 33 and 10 miRNAs were found to be statistically significant upregulated and downregulated under hypoxia, respectively. Bioinformatics analysis highlighted that a large number of hypoxia-suppressed miRNAs that target a variety of steroidogenic enzymes including steroidogenic acute regulatory protein, aromatase, and 17-alpha-monooxygenase. Also, estrogen receptor 2 and androgen receptor were found to be targeted by hypoxia-responsive miRNAs. For the first time, our results showed that hypoxia may upregulate specific steroidogenic enzymes and hormone receptors through actions of miRNA, and hence provide a novel mechanism for the observed female reproductive impairment caused by hypoxia.
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region; State Key Laboratory in Marine Pollution, Hong Kong. Electronic address: balllai@hku.hk.
authors.forename: Keng Po
authors.initials: KP
authors.lastname: Lai
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong , Hong Kong Special Administrative Region. Electronic address: marcoli@cuhk.edu.hk.
authors.forename: Jing-Woei
authors.initials: JW
authors.lastname: Li
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region; State Key Laboratory in Marine Pollution, Hong Kong. Electronic address: annatse@hku.hk.
authors.forename: Anna Chung-Kwan
authors.initials: AC
authors.lastname: Tse
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong , Hong Kong Special Administrative Region. Electronic address: tf.chan@cuhk.edu.hk.
authors.forename: Ting-Fung
authors.initials: TF
authors.lastname: Chan
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region; State Key Laboratory in Marine Pollution, Hong Kong. Electronic address: rudolfwu@hku.hk.
authors.forename: Rudolf Shiu-Sun
authors.initials: RS
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D029941:Fish Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology; D000860:Hypoxia / Q000503:physiopathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009990:Oryzias / Q000502:physiology*; D010053:Ovary / Q000378:metabolism; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D017423:Sequence Analysis, RNA; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D029941:Fish Proteins; D035683:MicroRNAs; D013256:Steroids
keywords: Fish; Hypoxia; MicroRNAs; Ovary; Steroidogenesis