title: [Optimization of Polysaccharide Extraction from Spirodela polyrrhiza by Plackett-Burman Design Combined with Box-Behnken Response Surface Methodology].
abstract: OBJECTIVE
  To optimize the processing of polysaccharide extraction from Spirodela polyrrhiza.
  
  
  METHODS
  Five factors related to extraction rate of polysaccharide were optimized by the Plackett-Burman design. Based on this study, three factors, including alcohol volume fraction, extraction temperature and ratio of material to liquid, were regarded as investigation factors by Box-Behnken response surface methodology.
  
  
  RESULTS
  The effect order of three factors on the extraction rate of polysaccharide from Spirodela polyrrhiza were as follows: extraction temperature, alcohol volume fraction,ratio of material to liquid. According to Box-Behnken response, the best extraction conditions were: alcohol volume fraction of 81%, ratio of material to liquid of 1:42, extraction temperature of 100 degrees C, extraction time of 60 min for four times.
  
  
  CONCLUSIONS
  Plackett-Burman design and Box-Behnken response surface methodology used to optimize the extraction process for the polysaccharide in this study is effective and stable.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Qi-nan
authors.initials: QN
authors.lastname: Wu
mesh_terms: D029064:Araceae / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*; D013696:Temperature
chemical_list: D011134:Polysaccharides