title: Diversity and homogeneity of oral microbiota in healthy Korean pre-school children using pyrosequencing.
abstract: Objectives The purpose of this study was designed to identify the oral microbiota in healthy Korean pre-school children using pyrosequencing. Materials and methods Dental plaque samples were obtained form 10 caries-free pre-school children. The samples were analysed using pyrosequencing. Results The pyrosequencing analysis revealed that, at the phylum level, Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria and Fusobacteria showed high abundance. Also, predominant genera were identified as core microbiome, such as Streptococcus, Neisseria, Capnocytophaga, Haemophilus and Veilonella. Conclusions The diversity and homogeneity was shown in the dental plaque microbiota in healthy Korean pre-school children.
authors.affiliation: a Department of Pediatric Dentistry, School of Dentistry , Kyung Hee University , Seoul , Korea ;
authors.forename: Soo Eon
authors.initials: SE
authors.lastname: Lee
authors.affiliation: a Department of Pediatric Dentistry, School of Dentistry , Kyung Hee University , Seoul , Korea ;|b Department of Dentistry, Graduate School , Kyung Hee University , Seoul , Korea.
authors.forename: Ok Hyung
authors.initials: OH
authors.lastname: Nam
authors.affiliation: a Department of Pediatric Dentistry, School of Dentistry , Kyung Hee University , Seoul , Korea ;
authors.forename: Hyo-Seol
authors.initials: HS
authors.lastname: Lee
authors.affiliation: a Department of Pediatric Dentistry, School of Dentistry , Kyung Hee University , Seoul , Korea ;
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Choi
mesh_terms: D039903:Actinobacteria / Q000145:classification; D001419:Bacteria / Q000145:classification*; D041963:Bacteroidetes / Q000145:classification; D002206:Capnocytophaga / Q000145:classification; D002675:Child, Preschool; D003731:Dental Caries / Q000382:microbiology; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D000068536:Bacillota / Q000145:classification; D045182:Fusobacteria / Q000145:classification; D006190:Haemophilus / Q000145:classification; D006801:Humans; D008297:Male; D059013:Microbial Consortia; D064307:Microbiota; D009055:Mouth / Q000382:microbiology*; D009343:Neisseria / Q000145:classification; D010865:Pilot Projects; D020560:Proteobacteria / Q000145:classification; D056910:Republic of Korea; D013291:Streptococcus / Q000145:classification; D014678:Veillonella / Q000145:classification
keywords: Oral microbiota; healthy children; homogeneity; microbial diversity; primary tooth