title: Preparation of cryogel columns for depletion of hemoglobin from human blood.
abstract: In this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.
authors.affiliation: a Biochemistry Division, Department of Chemistry, Faculty of Science , Hacettepe University , Ankara , Turkey ;
authors.forename: Ali
authors.initials: A
authors.lastname: Derazshamshir
authors.affiliation: b Biochemistry Division, Department of Chemistry , Aksaray University , Aksaray , Turkey ;
authors.forename: Gözde
authors.initials: G
authors.lastname: Baydemir
authors.affiliation: c Vocational School of Gerede Chemistry Technology Division , Abant Izzet Baysal University , Bolu , Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Yılmaz
authors.affiliation: a Biochemistry Division, Department of Chemistry, Faculty of Science , Hacettepe University , Ankara , Turkey ;
authors.forename: Nilay
authors.initials: N
authors.lastname: Bereli
authors.affiliation: a Biochemistry Division, Department of Chemistry, Faculty of Science , Hacettepe University , Ankara , Turkey ;
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D059985:Cryogels / Q000737:chemistry*; D004811:Epichlorohydrin / Q000737:chemistry; D006454:Hemoglobins / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D007098:Imino Acids / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*
chemical_list: D059985:Cryogels; D006454:Hemoglobins; D007098:Imino Acids; D004811:Epichlorohydrin; D011102:Polyhydroxyethyl Methacrylate; C008109:iminodiacetic acid
keywords: Hemoglobin depletion; IDA; PHEMA cryogel; metal chelate affinity sorbents