title: Roles of phagocytosis activating protein (PAP) in Aeromonas hydrophila infected Cyprinus carpio.
abstract: Cyprinus carpio (koi) is one of the most popular ornamental fish. A major problem for C. carpio farming is bacterial infections especially by Aeromonas hydrophila. Previously studies had shown that the Phagocytosis Activating Protein (PAP) gene was involved in the innate immune response of animals. Therefore, we attempted to identify a role for the PAP gene in the immunology of C. carpio. The expression of the PAP was found in C. carpio whole blood and increased when the fish were stimulated by inactivated A. hydrophila. In addition, PAP-phMGFP DNA was injected as an immunostimulant. The survival rate and the phagocytic index were significantly increased in the A. hydrophila infected fish that received the PAP-phMGFP DNA immunostimulant. A chitosan-PAP-phMGFP nanoparticle was then developed and feeded into fish which infected with A. hydrophila. These fish had a significantly lower mortality rate than the control. Therefore, this research confirmed a key role for PAP in protection fish from bacterial infection and the chitosan-PAP-phMGFP nanoparticle could be a good prototype for fish immunostimulant in the future.
authors.affiliation: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Dept. of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Monwadee
authors.initials: M
authors.lastname: Wonglapsuwan
authors.affiliation: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Dept. of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Pataraporn
authors.initials: P
authors.lastname: Kongmee
authors.affiliation: Dept. of Aquatic Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Naraid
authors.initials: N
authors.lastname: Suanyuk
authors.affiliation: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Dept. of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. Electronic address: wilaiwan58@hotmail.com.
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Chotigeat
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000276:immunology*; D002347:Carps / Q000276:immunology* / Q000382:microbiology*; D048271:Chitosan / Q000737:chemistry; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology; D007113:Immunity, Innate / Q000276:immunology*; D053758:Nanoparticles / Q000737:chemistry; D010587:Phagocytosis / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D001798:Blood Proteins; D029941:Fish Proteins; D048271:Chitosan
keywords: Aeromonas hydrophila; Carp; Chitosan; PAP; Phagocytosis; Ribosomal protein L26