title: Regeneration of tracheal epithelium using mouse induced pluripotent stem cells.
abstract: Conclusion The findings demonstrated the potential use of induced pluripotent stem cells for regeneration of tracheal epithelium. Objective Autologous tissue implantation techniques using skin or cartilage are often applied in cases of tracheal defects with laryngeal inflammatory lesions and malignant tumor invasion. However, these techniques are invasive with an unstable clinical outcome. The purpose of this study was to investigate regeneration in a tracheal defect site of nude rats after implantation of ciliated epithelium that was differentiated from induced pluripotent stem cells. Method Embryoid bodies were formed from mouse induced pluripotent stem cells. They were cultured with growth factors for 5 days, and then cultured at the air-liquid interface. The degree of differentiation achieved prior to implantation was determined by histological findings and the results of real-time polymerase chain reaction. Embryoid bodies including ciliated epithelium were embedded into collagen gel that served as an artificial scaffold, and then implanted into nude rats, creating an 'air-liquid interface model'. Histological evaluation was performed 7 days after implantation. Results The ciliated epithelial structure survived on the lumen side of regenerated tissue. It was demonstrated histologically that the structure was composed of ciliated epithelial cells.
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ikeda
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Imaizumi
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Susumu
authors.initials: S
authors.lastname: Yoshie
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Koshi
authors.initials: K
authors.lastname: Otsuki
authors.affiliation: b Department of Cellular and Integrative Physiology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Masao
authors.initials: M
authors.lastname: Miyake
authors.affiliation: b Department of Cellular and Integrative Physiology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hazama
authors.affiliation: c Department of Cell Science, Institute of Biomedical Sciences , Fukushima Medical University , Fukushima , Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Wada
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan ;
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000502:physiology; D057026:Induced Pluripotent Stem Cells / Q000502:physiology / Q000637:transplantation*; D008297:Male; D051379:Mice; D011916:Rats, Inbred F344; D011923:Rats, Nude; D012038:Regeneration; D020545:Respiratory Mucosa / Q000502:physiology*; D014133:Tracheal Diseases / Q000628:therapy*
keywords: Induced pluripotent stem cells; ciliated epithelial cells; embryoid body; regeneration