title: Determination of bisphenol A-glucuronide in human urine using ultrahigh-pressure liquid chromatography/tandem mass spectrometry.
abstract: BACKGROUND
  Used widely as a plasticizer and as a monomer for plastics, bisphenol A (BPA) is under investigation as a possible endocrine disrupter. As an indication of systemic exposure, a fast and accurate assay was developed for the major BPA metabolite in human urine, BPA-monoglucuronide (BPA-G), using ultrahigh-pressure liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS).
  
  
  METHODS
  Urine samples were prepared using solid-phase mixed-mode reversed-phase/anion-exchange extraction. BPA-G was measured using UHPLC/MS/MS with an amide UHPLC column interfaced to a triple-quadrupole mass spectrometer equipped with negative ion electrospray, collision-induced dissociation and selected reaction monitoring. [(13) C12 ]-BPA-G was used as a surrogate standard.
  
  
  RESULTS
  By measuring the glucuronide metabolite of BPA, potential interference due to BPA contamination from containers, solvents, pipette, etc., was avoided. The standard curve had a linear regression coefficient of 0.999, and the intra- and inter-assay variations were less than 10%. The assay was validated according to FDA guidelines.
  
  
  CONCLUSIONS
  A fast, accurate, and highly selective method for the determination of BPA-G in human urine was developed and validated using UHPLC/MS/MS. This method is suitable for assessing human exposure to BPA. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, 833 S. Wood Street, M/C 781, Chicago, IL, 60612, USA.
authors.forename: Zane Z
authors.initials: ZZ
authors.lastname: Hauck
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, 833 S. Wood Street, M/C 781, Chicago, IL, 60612, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, 833 S. Wood Street, M/C 781, Chicago, IL, 60612, USA.
authors.forename: Guannan
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, 833 S. Wood Street, M/C 781, Chicago, IL, 60612, USA.
authors.forename: Richard B
authors.initials: RB
authors.lastname: van Breemen
mesh_terms: D001559:Benzhydryl Compounds / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000652:urine; D020719:Glucuronides / Q000652:urine*; D006801:Humans; D010636:Phenols / Q000652:urine*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D020719:Glucuronides; D010636:Phenols; C512161:bisphenol A glucuronide; D000098826:Bisphenol A Compounds