title: The Responses of Tissues from the Brain, Heart, Kidney, and Liver to Resuscitation following Prolonged Cardiac Arrest by Examining Mitochondrial Respiration in Rats.
abstract: Cardiac arrest induces whole-body ischemia, which causes damage to multiple organs. Understanding how each organ responds to ischemia/reperfusion is important to develop better resuscitation strategies. Because direct measurement of organ function is not practicable in most animal models, we attempt to use mitochondrial respiration to test efficacy of resuscitation on the brain, heart, kidney, and liver following prolonged cardiac arrest. Male Sprague-Dawley rats are subjected to asphyxia-induced cardiac arrest for 30 min or 45 min, or 30 min cardiac arrest followed by 60 min cardiopulmonary bypass resuscitation. Mitochondria are isolated from brain, heart, kidney, and liver tissues and examined for respiration activity. Following cardiac arrest, a time-dependent decrease in state-3 respiration is observed in mitochondria from all four tissues. Following 60 min resuscitation, the respiration activity of brain mitochondria varies greatly in different animals. The activity after resuscitation remains the same in heart mitochondria and significantly increases in kidney and liver mitochondria. The result shows that inhibition of state-3 respiration is a good marker to evaluate the efficacy of resuscitation for each organ. The resulting state-3 respiration of brain and heart mitochondria following resuscitation reenforces the need for developing better strategies to resuscitate these critical organs following prolonged cardiac arrest.
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Junhwan
authors.initials: J
authors.lastname: Kim
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: José Paul Perales
authors.initials: JP
authors.lastname: Villarroel
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Tai
authors.initials: T
authors.lastname: Yin
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Shinozaki
authors.affiliation: College of Liberal Arts and Sciences, Villanova University, Villanova, PA 19085, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Hong
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Joshua W
authors.initials: JW
authors.lastname: Lampe
authors.affiliation: Center for Resuscitation Science, Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Lance B
authors.initials: LB
authors.lastname: Becker
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001921:Brain / Q000473:pathology*; D016887:Cardiopulmonary Resuscitation; D019069:Cell Respiration; D006321:Heart / Q000503:physiopathology*; D006323:Heart Arrest / Q000473:pathology*; D006339:Heart Rate; D007668:Kidney / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D017207:Rats, Sprague-Dawley