title: In Vitro Antibiofilm Activity of an Exopolysaccharide from the Marine Thermophilic Bacillus licheniformis T14.
abstract: The development of antibiofilm strategies is of major interest in contrasting bacterial pathogenic biofilms. A novel fructose and fucose rich exopolysaccharide (EPS1-T14) produced by the recently described thermophilic Bacillus licheniformis T14, isolated from a shallow hydrothermal vent of Panarea Island (Eolian Island, Italy), was evaluated for its effects on biofilm formation by multiresistant clinical strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The antibiofilm activity of EPS1-T14 was assessed by microtiter plate assays and visualized by confocal laser scanning microscopic images. EPS1-T14, with molecular weight of 1000 kDa, reduced biofilm formation on abiotic surfaces without affecting bacterial vitality. The novel EPS1-T14 is a water-soluble, noncytotoxic exopolymer able to prevent biofilm formation and its use may represent a promising therapeutic strategy for combating bacterial biofilm-associated infections. EPS1-T14 as antiadhesive biomolecule could be useful for novel prospective in medical and nonmedical applications.
authors.affiliation: Research Centre for Extreme Environments and Extremophiles, Department of Biological and Environmental Sciences, University of Messina, V.le F. Stagno d'Alcontres 31, 98166, Messina, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Spanò
authors.affiliation: Department of Biomedical Sciences and Morphological and Functional Images, University of Messina, V. Consolare Valeria, 98125, Messina, Italy.
authors.forename: Pasqualina
authors.initials: P
authors.lastname: Laganà
authors.affiliation: Department of Biomedical Sciences and Morphological and Functional Images, University of Messina, V. Consolare Valeria, 98125, Messina, Italy.
authors.forename: Giuseppa
authors.initials: G
authors.lastname: Visalli
authors.affiliation: Research Centre for Extreme Environments and Extremophiles, Department of Biological and Environmental Sciences, University of Messina, V.le F. Stagno d'Alcontres 31, 98166, Messina, Italy.
authors.forename: Teresa L
authors.initials: TL
authors.lastname: Maugeri
authors.affiliation: Research Centre for Extreme Environments and Extremophiles, Department of Biological and Environmental Sciences, University of Messina, V.le F. Stagno d'Alcontres 31, 98166, Messina, Italy. cgugliandolo@unime.it.
authors.forename: Concetta
authors.initials: C
authors.lastname: Gugliandolo
mesh_terms: D000069976:Bacillus licheniformis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D060086:Hydrothermal Vents / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D011135:Polysaccharides, Bacterial