title: Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland.
abstract: Two halophilic archaea, designated strains WSM-64(T) and WSM-66, were isolated from a sample taken from a borehole in the currently unexploited Barycz mining area belonging to the "Wieliczka" Salt Mine Company, in Poland. Strains are red pigmented and form non-motile cocci that stain Gram-negative. Strains WSM-64(T) and WSM-66 showed optimum growth at 40 °C, in 20% NaCl and at pH 6.5-7.5. The strains were facultative anaerobes. The major polar lipids of the two strains were phosphatidylglycerol (PG2), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulfated diglycosyl diether (S-DGD). Menaquinone MK-8 was the major respiratory quinone. The DNA G+C content of strain WSM-64(T) was 61.2 mol% by HPLC method; 61.0 mol% by genome sequencing. Analysis of the almost complete 16S rRNA gene sequence indicated that the strains WSM-64(T) and WSM-66 (99.7% identity) represented a member of the genus Halorhabdus in the family Halobacteriaceae. Both strains formed a distinct cluster and were most closely related to Halorhabdus tiamatea SARL4B(T) and Halorhabdus utahensis AX-2(T) (DSM 12940(T)) (95.4% and 95.6%, respectively). ANI values of WSM-64(T) with the closest relative type strains were <78.5%. Based on 16S rRNA gene sequence and whole genome analyses, physiological and biochemical characteristics we describe a new species represented by strain WSM-64(T) (=DSM 29498(T) =CECT 8673(T)) for which we propose the name Halorhabdus rudnickae sp. nov.
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
authors.forename: Luciana
authors.initials: L
authors.lastname: Albuquerque
authors.affiliation: Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kowalewicz-Kulbat
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Drzewiecka
authors.affiliation: Department of Genetics of Microorganisms, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Stączek
authors.affiliation: Área de Genómica y Salud, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública), Avenida de Cataluña 21, 46020 Valencia, Spain; Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, C/Catedrático José Beltrán 2, 46980 Valencia, Paterna, Spain.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: d'Auria
authors.affiliation: Marine Microbiology Group, Institut Mediterrani d'Estudis Avançats (CSIC-UIB), E-07190 Esporles, Illes Balears, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Rosselló-Móra
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal; Microbiology Unit, BIOCANT Biotechnological Park, 3060-197 Cantanhede, Portugal. Electronic address: milton@ci.uc.pt.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D015373:Bacterial Typing Techniques; D019015:Geologic Sediments / Q000382:microbiology*; D006216:Halobacteriaceae / Q000145:classification* / Q000302:isolation & purification; D011044:Poland
keywords: Haloarchaea; Halorhabdus; Halorhabdus rudnickae sp. nov