title: Nanoliposomal Growth Hormone and Sodium Nitrite Release from Silicone Fibers Reduces Thrombus Formation Under Flow.
abstract: Biocompatibility of artificial lungs can be improved by endothelialization of hollow fibers. Bioavailability of growth-inducing and anti-thrombotic agents on the hollow fiber-blood interface inhibits thrombosis. We investigated if nanoliposomal growth-inducing growth hormone (nGH) and anti-thrombotic sodium nitrite (nNitrite) incorporation into collagen-coating on silicone hollow fibers improves blood biocompatibility by increasing endothelial cell growth and nitrite bioavailability under flow. Nitrite production rate was assessed under varying flow conditions. Finite element (FE) modeling was used to simulate nitrite transport within the parallel-plate flow chamber, and nitrite bioavailability on the fiber-blood interface at 1-30 dyn/cm(2) shear stress. Endothelial cell number on fibers coated with nNitrite-nGH-collagen conjugate was 1.5-fold higher than on collagen-coated fibers. For collagen-coated fibers, nitrite production reached a maximum at 18 dyn/cm(2) shear stress. When fibers were coated with nNitrite-nGH-collagen conjugate, nitrite production increased continuously by increasing shear stress. FE modeling revealed that nitrite concentrations at the fiber-blood interface were affected by shear stress-induced nitrite production, and diffusion/convection-induced nitrite removal. Highest nitrite concentrations and lowest thrombus deposition were observed on fibers coated with nNitrite-nGH-collagen conjugate exposed to 6-12 dyn/cm(2) shear stress. In conclusion, our results suggest that nNitrite-nGH-Col conjugate coatings promote endothelialization of silicone hollow fibers in biohybrid artificial lungs.
authors.affiliation: School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11365-4563, Enqelab Avenue, Tehran, Iran.|Research Center for New Technologies in Life Science Engineering, University of Tehran, 143951374, Enqelab Avenue, Tehran, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Salehi-Nik
authors.affiliation: School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11365-4563, Enqelab Avenue, Tehran, Iran. amoabediny@ut.ac.ir.|Research Center for New Technologies in Life Science Engineering, University of Tehran, 143951374, Enqelab Avenue, Tehran, Iran. amoabediny@ut.ac.ir.
authors.forename: Ghassem
authors.initials: G
authors.lastname: Amoabediny
authors.affiliation: School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11365-4563, Enqelab Avenue, Tehran, Iran.|Research Center for New Technologies in Life Science Engineering, University of Tehran, 143951374, Enqelab Avenue, Tehran, Iran.
authors.forename: Seyedeh Parnian
authors.initials: SP
authors.lastname: Banikarimi
authors.affiliation: Department of Orthopedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.|Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, Mekelweg 2, 2628 CD, Delft, The Netherlands.
authors.forename: Behdad
authors.initials: B
authors.lastname: Pouran
authors.affiliation: Research Center for New Technologies in Life Science Engineering, University of Tehran, 143951374, Enqelab Avenue, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Malaie-Balasi
authors.affiliation: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Zandieh-Doulabi
authors.affiliation: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
authors.forename: Jenneke
authors.initials: J
authors.lastname: Klein-Nulend
mesh_terms: D021522:Bioartificial Organs; D040761:Biomimetic Materials / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001783:Blood Flow Velocity; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D019382:Human Growth Hormone / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008081:Liposomes; D008168:Lung / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D008955:Models, Cardiovascular; D049329:Nanostructures / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D012977:Sodium Nitrite / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D013927:Thrombosis / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D003692:Delayed-Action Preparations; D008081:Liposomes; D019382:Human Growth Hormone; D012977:Sodium Nitrite; D012825:Silicon
keywords: Biohybrid artificial lungs; Biomimetic collagen coating; Endothelialization; Finite element modeling; Nitrite bioavailability; Shear stress; Silicone hollow fibers; Thrombus deposition