title: Fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study.
abstract: OBJECTIVE
  Intraductal papillary mucinous neoplasm (IPMN) is an intraductal mucin-producing pancreatic neoplasm with the potential for malignant transformation. Changes in glycans expressed on the cell surface and glycotransferases play important roles in malignant transformation. We conducted this study to analyze glycan alterations in IPMNs by using a lectin microarray and to identify the factors associated with altered glycans and their relationships with malignant transformation.
  
  
  METHODS
  Using a lectin microarray, we evaluated glycan expression in 22 samples of IPMN with carcinoma, obtained from curative resections performed in our department. We also used immunohistochemistry to investigate fucosyltransferase 8 (Fut 8) protein expression, which is associated with glycan alterations in IPMNs.
  
  
  RESULTS
  The lectin microarray demonstrated that only two lectins, Aleuria aurantia lectin (AAL) and Aspergillus oryzae L-fucose-specific lectin (AOL), which bind to fucose, exhibited significant sequential increases from normal pancreatic duct to adenoma and carcinoma. Similarly, Fut 8 protein expression, which is associated with AAL and AOL, sequentially and significantly increased from the normal pancreatic duct to adenoma and carcinoma.
  
  
  CONCLUSIONS
  Lectin microarray analysis suggested that fucosylation is associated with the malignant transformation of IPMNs.
authors.affiliation: Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan. kiminori@oita-u.ac.jp.
authors.forename: Kiminori
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohta
authors.affiliation: Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yada
authors.affiliation: Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Komori
authors.affiliation: Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Iwashita
authors.affiliation: Department of Pathology, Oita Prefectural Hospital, Oita, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kashima
authors.affiliation: Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Inomata
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D000369:Aged, 80 and over; D001236:Aspergillus oryzae; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002291:Carcinoma, Papillary / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005643:Fucose; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D037102:Lectins; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011134:Polysaccharides / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D037102:Lectins; D011134:Polysaccharides; C064549:lectin, Aleuria aurantia; D005643:Fucose; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase
keywords: AAL; AOL; Fucosylation; IPMN; Lectin microarray