title: A critical role of LAMP-1 in avian reovirus P10 degradation associated with inhibition of apoptosis and virus release.
abstract: Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. The ARV p10 protein, a viroporin responsible for the induction of cell syncytium formation and apoptosis, is rapidly degraded in host cells. However, the mechanism of p10 degradation and its relevance are still unclear. We report here the identification of cellular lysosome-associated membrane protein 1 (LAMP-1) as an interaction partner of p10 by yeast two-hybrid screening, immunoprecipitation and confocal microscopy assays. We found that rapid degradation of p10 was associated with ubiquitination. Importantly, ARV p10 degradation in host cells could be completely abolished by knockdown of LAMP-1 by siRNA, indicating that LAMP-1 is required for ARV p10 degradation in host cells. In contrast, overexpression of LAMP-1 facilitated p10 degradation. Furthermore, knockdown of LAMP-1 allowed p10 accumulation, enhancing p10-induced apoptosis and viral release. Thus, LAMP-1 plays a critical role in ARV p10 degradation associated with inhibition of apoptosis and viral release.
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: He
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China. vetwyq@cau.edu.cn.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China. vetwyq@cau.edu.cn.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China. vetwyq@cau.edu.cn.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China. sjzheng@cau.edu.cn.|Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China. sjzheng@cau.edu.cn.|College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China. sjzheng@cau.edu.cn.
authors.forename: Shijun J
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins; D006801:Humans; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism*; D030101:Orthoreovirus, Avian / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D057074:Virus Release / Q000502:physiology*
chemical_list: D052118:Lysosomal-Associated Membrane Protein 1; D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins