title: Novel technologies provide more engineering strategies for amino acid-producing microorganisms.
abstract: Traditionally, amino acid-producing strains were obtained by random mutagenesis and subsequent selection. With the development of genetic and metabolic engineering techniques, various microorganisms with high amino acid production yields are now constructed by rational design of targeted biosynthetic pathways. Recently, novel technologies derived from systems and synthetic biology have emerged and open a new promising avenue towards the engineering of amino acid production microorganisms. In this review, these approaches, including rational engineering of rate-limiting enzymes, real-time sensing of end-products, pathway optimization on the chromosome, transcription factor-mediated strain improvement, and metabolic modeling and flux analysis, were summarized with regard to their application in microbial amino acid production.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Tianyuan
authors.initials: T
authors.lastname: Su
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China. qiqingsheng@sdu.edu.cn.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification* / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D000596:Amino Acids
keywords: Amino acids; Microorganisms; Novel technologies; Synthetic biology