title: Presepsin (sCD14-ST) secretion and kinetics by peripheral blood mononuclear cells and monocytic THP-1 cell line.
abstract: Presepsin could help for early diagnosis of systemic infection. Little is known regarding its kinetics. We studied presepsin concentration after challenge with bacterial agonist lipopolysaccharide (LPS) stimulation in peripheral mononuclear cells (PMNC) collected from 5 healthy volunteers and in a human cell line of monocytic cells (THP1). In PMNC, an exposure to LPS (100 ng/mL) induced an increase of median presepsin levels as early as hour 1 (+31%, p=0.007), concomitantly to IL-6 synthesis. In THP1 cells, presepsin was detected at 1 hour after LPS exposure, and peaked at 3 hours, in THP1 cells. In conclusion, we report here that presepsin, a surrogate marker of the host response to bacteria, increases early in PMNC and in a monocytic cell lineage. Our findings might confirm the potential usefulness of presepsin bedside as an early marker of infectious diseases.
authors.affiliation: Service de diagnostic biologique automatisé, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Chenevier-Gobeaux
authors.affiliation: Service d'hématologie, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France., Faculté de médecine, Université Paris Descartes, Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Bardet
authors.affiliation: Service de bactériologie, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Poupet
authors.affiliation: Service de bactériologie, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Poyart
authors.affiliation: Service de diagnostic biologique automatisé, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France, UMR 1124 pharmacologie, toxicologie et signalisation cellulaire, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Borderie
authors.affiliation: Service d'accueil des urgences, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France.
authors.forename: Yann-Erick
authors.initials: YE
authors.lastname: Claessens
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006801:Humans; D007239:Infections / Q000097:blood / Q000175:diagnosis; D015850:Interleukin-6 / Q000096:biosynthesis; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D018805:Sepsis / Q000097:blood / Q000175:diagnosis
chemical_list: D015415:Biomarkers; C508600:IL6 protein, human; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D010446:Peptide Fragments; C000594167:presepsin protein, human
keywords: biomarkers; lipopolysaccharide; presepsin; sepsis