title: Production of genome-edited pluripotent stem cells and mice by CRISPR/Cas.
abstract: Clustered regularly at interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nucleases, so-called CRISPR/Cas, was recently developed as an epoch-making genome engineering technology. This system only requires Cas9 nuclease and single-guide RNA complementary to a target locus. CRISPR/Cas enables the generation of knockout cells and animals in a single step. This system can also be used to generate multiple mutations and knockin in a single step, which is not possible using other methods. In this review, we provide an overview of genome editing by CRISPR/Cas in pluripotent stem cells and mice.
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Horii
authors.forename: Izuho
authors.initials: I
authors.lastname: Hatada
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D053595:Embryonic Stem Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D000072669:Gene Editing / Q000639:trends; D055879:Gene Knock-In Techniques / Q000639:trends; D055786:Gene Knockout Techniques / Q000639:trends; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D009030:Mosaicism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism