title: Microsatellite analysis of Saccharomyces uvarum diversity.
abstract: Considered as a sister species of Saccharomyces cerevisiae, S. uvarum is, to a lesser extent, an interesting species for fundamental and applied research studies. Despite its potential interest as a new gene pool for fermenting agents, the intraspecific molecular genetic diversity of this species is still poorly investigated. In this study, we report the use of nine microsatellite markers to describe S. uvarum genetic diversity and population structure among 108 isolates from various geographical and substrate origins (wine, cider and natural sources). Our combined microsatellite markers set allowed differentiating 89 genotypes. In contrast to S. cerevisiae genetic diversity, wild and human origin isolates were intertwined. A total of 75% of strains were proven to be homozygotes and estimated heterozygosity suggests a selfing rate above 0.95 for the different population tested here. From this point of view, the S. uvarum life cycle appears to be more closely related to S. paradoxus or S. cerevisiae of natural resources than S. cerevisiae wine isolates. Population structure could not be correlated to distinct geographic or technological origins, suggesting lower differentiation that may result from a large exchange between human and natural populations mediated by insects or human activities.
authors.affiliation: Université de Bordeaux, Unité de recherche Œnologie, ISVV, EA 4577, USC 1366 INRA, Villenave d'Ornon, France Bordeaux Sciences Agro, 1 cours du Général de Gaulle, 33170 Gradignan, France isabelle.masneuf@agro-bordeaux.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Masneuf-Pomarede
authors.affiliation: INRA, UMR1202 Biodiversité Gènes et Ecosystèmes, PlateForme Génomique, Cestas 33610, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Salin
authors.affiliation: Université de Bordeaux, Unité de recherche Œnologie, ISVV, EA 4577, USC 1366 INRA, Villenave d'Ornon, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Börlin
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Coton
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané
authors.forename: Monika
authors.initials: M
authors.lastname: Coton
authors.affiliation: Université de Haute Alsace, Laboratoire Vigne Biotechnologie et Environnement, 68000 Colmar, France.
authors.forename: Christine Le
authors.initials: CL
authors.lastname: Jeune
authors.affiliation: INRA, UMR1083 Science pour l'Œnologie, Montpellier, F-34060, France SupAgro, UMR1083, Montpellier, F-34060, France Université Montpellier, UMR1083, Montpellier, F-34060, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Legras
mesh_terms: D005516:Food Microbiology; D014644:Genetic Variation; D018895:Microsatellite Repeats; D010944:Plants / Q000382:microbiology; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics*
keywords: Saccharomyces uvarum; genetic diversity; microsatellite markers; population structure