title: The substrate promiscuity of a phosphopantetheinyl transferase SchPPT for coenzyme A derivatives and acyl carrier proteins.
abstract: Phosphopantetheinyl transferases (PPTases) catalyze the posttranslational modification of acyl carrier proteins (ACPs) in fatty acid synthases (FASs), ACPs in polyketide synthases, and peptidyl carrier proteins (PCPs) in nonribosomal peptide synthetases (NRPSs) in all organisms. Some bacterial PPTases have broad substrate specificities for ACPs/PCPs and/or coenzyme A (CoA)/CoA analogs, facilitating their application in metabolite production in hosts and/or labeling of ACPs/PCPs, respectively. Here, a group II PPTase SchPPT from Streptomyces chattanoogensis L10 was characterized to accept a heterologous ACP and acetyl-CoA. Thus, SchPPT is a promiscuous PPTase and may be used on polyketide production in heterologous bacterial host and labeling of ACPs.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors.forename: Yue-Yue
authors.initials: YY
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors.forename: Hong-Dou
authors.initials: HD
authors.lastname: Luo
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors.forename: Xiao-Sheng
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: Shanghai Aobopharmtech Inc. Ltd., Shanghai, 201203, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lin
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China. jianghuisioc@zju.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003065:Coenzyme A / Q000737:chemistry* / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism*
chemical_list: D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; C082662:phosphopantetheinyl transferase; D017855:Transferases (Other Substituted Phosphate Groups); D003065:Coenzyme A
keywords: Acyl carrier protein; Coenzyme A; Phosphopantetheinyl transferases; Streptomyces chattanoogensis; Substrate specificity