title: Surface-modified yeast cells: A novel eukaryotic carrier for oral application.
abstract: The effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.
authors.affiliation: Molecular and Cell Biology, Saarland University, D-66123 Saarbrücken, Germany.
authors.forename: Elisabeth E
authors.initials: EE
authors.lastname: Kenngott
authors.affiliation: Molecular and Cell Biology, Saarland University, D-66123 Saarbrücken, Germany.
authors.forename: Ruth
authors.initials: R
authors.lastname: Kiefer
authors.affiliation: Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), D-66123 Saarbrücken, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Schneider-Daum
authors.affiliation: Charité Universitätsmedizin Berlin and Deutsches Rheuma-Forschungszentrum, D-10117, Berlin, Germany.
authors.forename: Alf
authors.initials: A
authors.lastname: Hamann
authors.affiliation: Department of Pharmacy, Biopharmaceutics and Pharmaceutical Technology, Saarland University, D-66123 Saarbrücken, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Schneider
authors.affiliation: Molecular and Cell Biology, Saarland University, D-66123 Saarbrücken, Germany.
authors.forename: Manfred J
authors.initials: MJ
authors.lastname: Schmitt
authors.affiliation: Molecular and Cell Biology, Saarland University, D-66123 Saarbrücken, Germany. Electronic address: fb@microbiol.uni-sb.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Breinig
mesh_terms: D000941:Antigens / Q000008:administration & dosage* / Q000737:chemistry*; D002460:Cell Line; D002473:Cell Wall / Q000737:chemistry; D018920:Coculture Techniques; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005057:Eukaryotic Cells; D006801:Humans; D019012:Integrin beta1; D008565:Membrane Proteins / Q000737:chemistry; D010581:Peyer's Patches / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D014014:Tissue Adhesives; D015008:Yersinia enterocolitica; D015011:Yersinia pseudotuberculosis
chemical_list: D000941:Antigens; D004337:Drug Carriers; D019012:Integrin beta1; D008565:Membrane Proteins; D014014:Tissue Adhesives
keywords: Cell surface-expression; Invasin; M cell; Oral delivery; Saccharomyces cerevisiae