title: A Novel Naturally Occurring Class I 5-Enolpyruvylshikimate-3-Phosphate Synthase from Janibacter sp. Confers High Glyphosate Tolerance to Rice.
abstract: As glyphosate is a broad spectrum herbicide extensively used in agriculture worldwide, identification of new aroA genes with high level of glyphosate tolerance is essential for the development and breeding of transgenic glyphosate-tolerant crops. In this study, an aroA gene was cloned from a Janibacter sp. strain isolated from marine sediment (designated as aroAJ. sp). The purified aroAJ. sp enzyme has a Km value of 30 μM for PEP and 83 μM for S3P, and a significantly higher Ki value for glyphosate (373 μM) than aroAE. coli. AroAJ. sp is characterized as a novel and naturally occurring class I aroA enzyme with glyphosate tolerance. Furthermore, we show that aroAJ. sp can be used as an effective selectable marker in both japonica and indica rice cultivar. Transgenic rice lines were tested by herbicide bioassay and it was confirmed that they could tolerate up to 3360 g/ha glyphosate, a dosage four-fold that of the recommended agricultural application level. To our knowledge, it is the first report of a naturally occurring novel class I aroA gene which can be efficiently utilized to study and develop transgenic glyphosate-tolerant crops, and can facilitate a more economical and simplified weed control system.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shu-yuan
authors.initials: SY
authors.lastname: Yi
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Zi-duo
authors.initials: ZD
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Lin
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics* / Q000378:metabolism; D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics; D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics; D000383:Agriculture; D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli; D005798:Genes, Bacterial; D005819:Genetic Markers; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006720:Homozygote; D007700:Kinetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014170:Transformation, Genetic; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D005819:Genetic Markers; D005998:Glycine; D000097797:Glyphosate