title: Correlation between polymorphism of endothelial nitric oxide synthase and avascular necrosis of femoral head.
abstract: OBJECTIVE
  We analyzed the correlation between mutation in intron 4 and exon 7 of endothelial nitric oxide synthase (eNOS) and avascular necrosis of femoral head (ANFH).
  
  
  METHODS
  A total of 260 ANFH cases without history of hip joint injuries were diagnosed and subject to staging according to Ficat standard, with 262 health subjects as control. Venous blood was collected to extract genome DNA, which was then amplified by PCR. The polymorphism of 27 bp repeat sequence in intron 4 and G894T polymorphism in exon 7 of eNOS gene was detected.
  
  
  RESULTS
  The b/b, b/a and a/a genotype frequency of intron 4 was 77.7%, 19.2% and 3.1% in ANFH group, respectively, and that in the control group was 58.0%, 32.8% and 9.2%, respectively. The b allele frequency in ANFH group was obviously higher than that in the control (P<0.0001). The frequency of 894 G/G wild type, G/T heterozygote and T/T homozygote in eNOS exon 7 was analyzed by PCR-RLFP: 65.4%, 26.5% and 8.1% in ANFH group, and 46.2%, 37.8% and 16% in normal control, respectively. The frequency of TT genotype in ANFH was obviously higher than that in the control group (P<0.001).
  
  
  CONCLUSIONS
  Polymorphism of eNOS was correlated with ANFH.
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Jing-Na
authors.initials: JN
authors.lastname: Sun
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Hai-Jing
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Chao-Hua
authors.initials: CH
authors.lastname: Zhu
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Cell Therapy Center, The First Hospital of Hebei Medical University No. 89 Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Quan-Hai
authors.initials: QH
authors.lastname: Li
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Zhen-Shuan
authors.initials: ZS
authors.lastname: Zhao
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Yu
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Liu
authors.affiliation: Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
keywords: Polymorphism/genetics; endothelial cells/enzymology; ischemia; necrosis of femoral head/genetics; nitric oxide synthase (NOS)/genetics