title: Binding of Sulpiride to Seric Albumins.
abstract: The aim of this work was to study the interaction of sulpiride with human serum albumin (HSA) and bovine serum albumin (BSA) through the fluorescence quenching technique. As sulpiride molecules emit fluorescence, we have developed a simple mathematical model to discriminate the quencher fluorescence from the albumin fluorescence in the solution where they interact. Sulpiride is an antipsychotic used in the treatment of several psychiatric disorders. We selectively excited the fluorescence of tryptophan residues with 290 nm wavelength and observed the quenching by titrating HSA and BSA solutions with sulpiride. Stern-Volmer graphs were plotted and quenching constants were estimated. Results showed that sulpiride form complexes with both albumins. Estimated association constants for the interaction sulpiride-HSA were 2.20 (±0.08) × 10⁴ M(-1), at 37 °C, and 5.46 (±0.20) × 10⁴ M(-1), at 25 °C. Those for the interaction sulpiride-BSA are 0.44 (±0.01) × 10⁴ M(-1), at 37 °C and 2.17 (±0.04) × 10⁴ M(-1), at 25 °C. The quenching intensity of BSA, which contains two tryptophan residues in the peptide chain, was found to be higher than that of HSA, what suggests that the primary binding site for sulpiride in albumin should be located next to the sub domain IB of the protein structure.
authors.affiliation: Laboratory of Innovations in Therapies, Education and Bioproducts, Oswaldo Cruz Institute/FIOCRUZ, Av. Brasil 4365, Rio de Janeiro 21045-900, Brazil. viviane.fragoso@ioc.fiocruz.br.
authors.forename: Viviane Muniz
authors.initials: VM
authors.lastname: da Silva Fragoso
authors.affiliation: Postgraduation in Medical Sciences, Rio de Janeiro State University, Av. Manoel de Abreu, 444, Rio de Janeiro 20550-171, Brazil. carla.morais@inca.gov.br.
authors.forename: Carla Patrícia
authors.initials: CP
authors.lastname: de Morais Coura
authors.affiliation: Laboratory of Innovations in Therapies, Education and Bioproducts, Oswaldo Cruz Institute/FIOCRUZ, Av. Brasil 4365, Rio de Janeiro 21045-900, Brazil. luanda.hoope@ioc.fiocruz.br.
authors.forename: Luanda Yanaan
authors.initials: LY
authors.lastname: Hoppe
authors.affiliation: Applied Mathematics, Rio de Janeiro State University, Rua São Francisco Xavier, 524, Rio de Janeiro 20559-900, Brazil. marilia.agsoares@gmail.com.
authors.forename: Marília Amável Gomes
authors.initials: MA
authors.lastname: Soares
authors.affiliation: Postgraduation in Medical Sciences, Rio de Janeiro State University, Av. Manoel de Abreu, 444, Rio de Janeiro 20550-171, Brazil. dilsons@uerj.br.|Applied Mathematics, Rio de Janeiro State University, Rua São Francisco Xavier, 524, Rio de Janeiro 20559-900, Brazil. dilsons@uerj.br.
authors.forename: Dilson
authors.initials: D
authors.lastname: Silva
authors.affiliation: Postgraduation in Medical Sciences, Rio de Janeiro State University, Av. Manoel de Abreu, 444, Rio de Janeiro 20550-171, Brazil. ccortezs@ime.uerj.br.|Applied Mathematics, Rio de Janeiro State University, Rua São Francisco Xavier, 524, Rio de Janeiro 20559-900, Brazil. ccortezs@ime.uerj.br.
authors.forename: Celia Martins
authors.initials: CM
authors.lastname: Cortez
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013469:Sulpiride / Q000378:metabolism*
chemical_list: D014150:Antipsychotic Agents; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013469:Sulpiride
keywords: albumins; fluorescence quenching; interaction; sulpiride