title: Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria.
abstract: We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Melissa
authors.initials: M
authors.lastname: Neo
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: David G
authors.initials: DG
authors.lastname: Morris
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Pawel
authors.initials: P
authors.lastname: Kuklik
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Dennis H
authors.initials: DH
authors.lastname: Lau
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Hany
authors.initials: H
authors.lastname: Dimitri
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Lim
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Prashanthan
authors.initials: P
authors.lastname: Sanders
authors.affiliation: Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.|Centre for Heart Rhythm Disorders (CHRD), South Australian Health and Medical Research Institute (SAHMRI), School of Medical Sciences, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Saint
mesh_terms: D000200:Action Potentials; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology; D016275:Atrial Function; D022062:Electrophysiologic Techniques, Cardiac / Q000295:instrumentation; D059907:Functional Neuroimaging; D006325:Heart Atria / Q000294:innervation* / Q000503:physiopathology; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials; D046228:Microarray Analysis; D008839:Microelectrodes; D009431:Neural Conduction; D010865:Pilot Projects; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013610:Tachycardia / Q000503:physiopathology
keywords: arrhythmogenic mechanisms; atrial action potential membrane recordings; atrial fibrillation; cartographie à haute densité; electrophysiology; enregistrements de potentiels d’action auriculaires; fibrillation auriculaire; high-density mapping; mécanismes proarythmiques; électrophysiologie