title: Upregulation of Mir-34a in AGS Gastric Cancer Cells by a PLGA-PEG-PLGA Chrysin Nano Formulation.
abstract: BACKGROUND
  Nano-therapy has the potential to revolutionize cancer therapy. Chrysin, a natural flavonoid, was recently recognized as having important biological roles in chemical defenses and nitrogen fixation, with anti-inflammatory and anti-oxidant effects but the poor water solubility of flavonoids limitstheir bioavailability and biomedical applications.
  
  
  OBJECTIVE
  Chrysin loaded PLGA-PEG-PLGA was assessed for improvement of solubility, drug tolerance and adverse effects and accumulation in a gastric cancer cell line (AGS).
  
  
  METHODS
  Chrysin loaded PLGA-PEG copolymers were prepared using the double emulsion method (W/O/W). The morphology and size distributions of the prepared PLGA-PEG nanospheres were investigated by 1H NMR, FT-IR and SEM. The in vitro cytotoxicity of pure and nano-chrysin was tested by MTT assay and miR-34a was measured by real-time PCR.
  
  
  RESULTS
  1H NMR, FT-IR and SEM confirmed the PLGA-PEG structure and chrysin loaded on nanoparticles. The MTT results for different concentrations of chrysin at different times for the treatment of AGS cell line showed IC50 values of 68.2, 56.2 and 42.3 μM and 58.2, 44.2, 36.8 μM after 24, 48, and 72 hours of treatment, respectively for chrysin itslef and chrysin-loaded nanoparticles. The results of real time PCR showed that expression of miR-34a was upregulated to a greater extent via nano chrysin rather than free chrysin.
  
  
  CONCLUSIONS
  Our study demonstrates chrysin loaded PLGA-PEG promises a natural and efficient system for anticancer drug delivery to fight gastric cancer.
authors.affiliation: Department of Medical Biotechnology, Faculty of Advance Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran E-mail : zarghami@tbzmed.ac.ir.
authors.forename: Farideh
authors.initials: F
authors.lastname: Mohammadian
authors.forename: Alireza
authors.initials: A
authors.lastname: Abhari
authors.forename: Hassan
authors.initials: H
authors.lastname: Dariushnejad
authors.forename: Faraz
authors.initials: F
authors.lastname: Zarghami
authors.forename: Alireza
authors.initials: A
authors.lastname: Nikanfar
authors.forename: Yones
authors.initials: Y
authors.lastname: Pilehvar-Soltanahmadi
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D004337:Drug Carriers; D005419:Flavonoids; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; C043561:chrysin; D011092:Polyethylene Glycols