title: Whitening Effect of Watersoluble Royal Jelly from South Korea.
abstract: Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
authors.forename: Sang Mi
authors.initials: SM
authors.lastname: Han
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
authors.forename: In Phyo
authors.initials: IP
authors.lastname: Hong
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
authors.forename: Soon Ok
authors.initials: SO
authors.lastname: Woo
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
authors.forename: Se Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
authors.forename: Hye Ri
authors.initials: HR
authors.lastname: Jang
authors.affiliation: Catholic University of Daegu School of Medicine, Daegu 42472, Korea.
authors.forename: Kwan Kyu
authors.initials: KK
authors.lastname: Park
authors.affiliation: School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW 2795, Australia.
authors.forename: Sok Cheon
authors.initials: SC
authors.lastname: Pak
keywords: TRP-1; TRP-2; royal jelly; tyrosinase; water-soluble royal jelly; α-MSH