title: Biodegradable nanofiber-membrane for sustainable release of lidocaine at the femoral fracture site as a periosteal block: In vitro and in vivo studies in a rabbit model.
abstract: The aim of this study was to evaluate the efficacy of a biodegradable, lidocaine-embedded, nanofibrous membrane for the sustainable analgesic release onto fragments of a segmental femoral fracture site. Membranes of three different lidocaine concentrations (10%, 30%, and 50%) were produced via an electrospinning technique. In vitro lidocaine release was assessed by high-performance liquid chromatography. A femoral segmental fracture, with intramedullary Kirschner-wire fixation and polycaprolactone stent enveloping the fracture site, was set-up in a rabbit model for in vivo assessment of post-operative recovery of activity. Eighteen rabbits were randomly assigned to three groups (six rabbits per group): group A comprised of rabbits with femoral fractures and underwent fixation; group B comprised of a comparable fracture model to that of group A with the implantation of lidocaine-loaded nanofibers; and group C, the control group, received only anesthesia. The following variables were measured: change in body weight, food and water intake before and after surgery, and total activity count post-surgery. All membranes eluted effective levels of lidocaine for more than 3 weeks post-surgery. Rabbits in group B showed faster recovery of activity post-operatively, compared with those in group A, which confirmed the pain relief efficacy of the lidocaine-embedded nanofibers. Nanofibers with sustainable lidocaine release have adequate efficacy and durability for pain relief in rabbits with segmental long bone fractures.
authors.affiliation: Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Ying-Chao
authors.initials: YC
authors.lastname: Chou
authors.affiliation: Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Yi-Shiun
authors.initials: YS
authors.lastname: Cheng
authors.affiliation: Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Yung-Heng
authors.initials: YH
authors.lastname: Hsu
authors.affiliation: Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Yi-Hsun
authors.initials: YH
authors.lastname: Yu
authors.affiliation: Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan. Electronic address: shihjung@mail.cgu.edu.tw.
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D020341:Absorbable Implants; D000779:Anesthetics, Local / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D004326:Drinking / Q000187:drug effects; D065546:Drug Liberation; D004435:Eating / Q000187:drug effects; D005264:Femoral Fractures / Q000503:physiopathology* / Q000601:surgery; D008012:Lidocaine / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009407:Nerve Block / Q000379:methods*; D010149:Postoperative Pain / Q000517:prevention & control; D010521:Periosteum / Q000294:innervation; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000779:Anesthetics, Local; D003692:Delayed-Action Preparations; D008567:Membranes, Artificial; D008012:Lidocaine
keywords: Biodegradable nanofiber; Periosteal block; Sustainable release of lidocaine