title: Structures of Xenopus Embryonic Epidermal Lectin Reveal a Conserved Mechanism of Microbial Glycan Recognition.
abstract: Intelectins (X-type lectins), broadly distributed throughout chordates, have been implicated in innate immunity. Xenopus laevis embryonic epidermal lectin (XEEL), an intelectin secreted into environmental water by the X. laevis embryo, is postulated to function as a defense against microbes. XEEL is homologous (64% identical) to human intelectin-1 (hIntL-1), which is also implicated in innate immune defense. We showed previously that hIntL-1 binds microbial glycans bearing exocyclic vicinal diol groups. It is unknown whether XEEL has the same ligand specificity. Also unclear is whether XEEL and hIntL-1 have similar quaternary structures, as XEEL lacks the corresponding cysteine residues in hIntL-1 that stabilize the disulfide-linked trimer. These observations prompted us to further characterize XEEL. We found that hIntL-1 and XEEL have similar structural features. Even without the corresponding intermolecular disulfide bonds present in hIntL-1, the carbohydrate recognition domain of XEEL (XEELCRD) forms a stable trimer in solution. The structure of XEELCRD in complex with d-glycerol-1-phosphate, a residue present in microbe-specific glycans, indicated that the exocyclic vicinal diol coordinates to a protein-bound calcium ion. This ligand-binding mode is conserved between XEEL and hIntL-1. The domain architecture of full-length XEEL is reminiscent of a barbell, with two sets of three glycan-binding sites oriented in opposite directions. This orientation is consistent with our observation that XEEL can promote the agglutination of specific serotypes of Streptococcus pneumoniae. These data support a role for XEEL in innate immunity, and they highlight structural and functional conservation of X-type lectins among chordates.
authors.affiliation: From the Departments of Chemistry.
authors.forename: Kittikhun
authors.initials: K
authors.lastname: Wangkanont
authors.affiliation: Biochemistry, and.
authors.forename: Darryl A
authors.initials: DA
authors.lastname: Wesener
authors.affiliation: Biochemistry, and.
authors.forename: Jack A
authors.initials: JA
authors.lastname: Vidani
authors.affiliation: From the Departments of Chemistry,; Biochemistry, and. Electronic address: kiessling@chem.wisc.edu.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Kiessling
authors.affiliation: Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706. Electronic address: forest@bact.wisc.edu.
authors.forename: Katrina T
authors.initials: KT
authors.lastname: Forest
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018360:Crystallography, X-Ray; D016207:Cytokines / Q000737:chemistry / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D016207:Cytokines; D058851:GPI-Linked Proteins; C432397:ITLN1 protein, human; C502725:ITLN2 protein, Xenopus; D037102:Lectins; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial
keywords: X-ray crystallography; bacterial agglutination; carbohydrate-binding protein; galactofuranose; glycerol phosphate; innate immunity; intelectin; lectin; omentin; protein cross-linking