title: Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.
abstract: Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.
authors.forename: Roberta Fogliatto
authors.initials: RF
authors.lastname: Mariot
authors.forename: Luisa Abruzzi
authors.initials: LA
authors.lastname: de Oliveira
authors.affiliation: RIKILT Food Safety Institute, Wageningen University , 6708 PB Wageningen, The Netherlands.
authors.forename: Marleen M
authors.initials: MM
authors.lastname: Voorhuijzen
authors.affiliation: RIKILT Food Safety Institute, Wageningen University , 6708 PB Wageningen, The Netherlands.
authors.forename: Martijn
authors.initials: M
authors.lastname: Staats
authors.affiliation: Plant Breeding, Wageningen UR , 6708 PB Wageningen, The Netherlands.
authors.forename: Ronald C B
authors.initials: RC
authors.lastname: Hutten
authors.affiliation: RIKILT Food Safety Institute, Wageningen University , 6708 PB Wageningen, The Netherlands.
authors.forename: Jeroen P
authors.initials: JP
authors.lastname: van Dijk
authors.affiliation: RIKILT Food Safety Institute, Wageningen University , 6708 PB Wageningen, The Netherlands.
authors.forename: Esther J
authors.initials: EJ
authors.lastname: Kok
authors.forename: Jeverson
authors.initials: J
authors.lastname: Frazzon
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis* / Q000633:toxicity; D053898:Biosynthetic Pathways; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012992:Solanine / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000470:Alkaloids; D010940:Plant Proteins; C514919:alpha-solanine; D012992:Solanine; C011860:alpha-chaconine
keywords: GAMEs; SGT1 and SGT3 genes; Solanum tuberosum; gene expression; glycoalkaloids