title: Use of the Integrated Biomarker Response to Measure the Effect of Short-term Exposure to Dibenz[a,h]anthracene in Common carp (Cyprinus carpio).
abstract: Dibenz[a,h]anthracene (DbA) is a polycyclic aromatic hydrocarbon that is released into the environment through incomplete combustion of gasoline, cigarettes, and coal tar. The effects of short-term (10 days) exposure of common carp (Cyprinus carpio) to DbA (0-50 µg L(-1)) were evaluated using the following four biomarkers: DNA damage, 7-ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, and vitellogenin (VTG) levels. An integrated biomarker response (IBR) was calculated for exposure to DbA, and the results were compared with those in our previous study of two other PAHs, benzo[k]fluoranthene (BkF) and benzo[a]pyrene (BaP). DbA exposure resulted in a significant (p < 0.05) increase in DNA damage, EROD activity, and VTG levels relative to the control. By contrast, DbA did not affect AChE activity. The IBR increased as the concentration of DbA increased. Based on the IBR values, the order of toxicity for the PAHs was BkF > BaP > DbA. Our results suggest that the IBR can be used as a quantitative tool for evaluating the responses of multiple biomarkers to PAH exposure.
authors.affiliation: Environmental Biology and Chemistry Research Center, Korea Institute of Toxicology, Jinju, 660-844, Korea.
authors.forename: Ja-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 305-343, Korea. wookkim@kitox.re.kr.
authors.forename: Woo-Keun
authors.identifier: http://orcid.org/0000-0001-8355-4057
authors.initials: WK
authors.lastname: Kim
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001551:Benz(a)Anthracenes / Q000032:analysis / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002347:Carps / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D013997:Time Factors; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D001551:Benz(a)Anthracenes; D015415:Biomarkers; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1; D000110:Acetylcholinesterase; C026486:1,2,5,6-dibenzanthracene
keywords: Biomarker; Common carp; Dibenz[ah]anthracene; Star plot