title: The effect of medium viscosity on kinetics of ATP hydrolysis by the chloroplast coupling factor CF1.
abstract: The coupling factor CF1 is a catalytic part of chloroplast ATP synthase which is exposed to stroma whose viscosity is many-fold higher than that of reaction mixtures commonly used to measure kinetics of CF1-catalyzed ATP hydrolysis. This study is focused on the effect of medium viscosity modulated by sucrose or bovine serum albumin (BSA) on kinetics of Ca(2+)- and Mg(2+)-dependent ATP hydrolysis by CF1. These agents were shown to reduce the maximal rate of Ca(2+)-dependent ATPase without changing the apparent Michaelis constant (К m), thus supporting the hypothesis on viscosity dependence of CF1 activity. For the sulfite- and ethanol-stimulated Mg(2+)-dependent reaction, the presence of sucrose increased К m without changing the maximal rate that is many-fold as high as that of Ca(2+)-dependent hydrolysis. The hydrolysis reaction was shown to be stimulated by low concentrations of BSA and inhibited by its higher concentrations, with the increasing maximal reaction rate estimated by extrapolation. Sucrose- or BSA-induced inhibition of the Mg(2+)-dependent ATPase reaction is believed to result from diffusion-caused deceleration, while its BSA-induced stimulation is probably caused by optimization of the enzyme structure. Molecular mechanisms of the inhibitory effect of viscosity are discussed. Taking into account high protein concentrations in the chloroplast stroma, it was suggested that kinetic parameters of ATP hydrolysis, and probably those of ATP synthesis in vivo as well, must be quite different from measurements taken at a viscosity level close to that of water.
authors.affiliation: Institute of Basic Biological Problems Russian Academy of Sciences, Pushchino, Russia. alexander.malyan@gmail.com.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Malyan
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002384:Catalysis / Q000187:drug effects; D025222:Chloroplast Proton-Translocating ATPases / Q000187:drug effects* / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D004058:Diffusion; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D018724:Spinacia oleracea / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology*; D014783:Viscosity / Q000187:drug effects
chemical_list: D003470:Culture Media; D012710:Serum Albumin, Bovine; D013395:Sucrose; D000255:Adenosine Triphosphate; D025222:Chloroplast Proton-Translocating ATPases; D008274:Magnesium; D002118:Calcium
keywords: ATP hydrolysis; Chloroplast coupling factor 1 (CF1); Viscosity