title: Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii.
abstract: The giant freshwater prawn, Macrobrachium rosenbergii, is important to many Asian countries due to its high economic value as an aquaculture product. With demand increasing, there is requirement for a better understanding of the biosynthetic components that regulate its growth and reproduction, including steroids, in order to help increase production. Vertebrate-type steroids and their receptors were identified in crustaceans and implicated in reproduction. In this study, we presented the sex steroids estradiol and progesterone by LC-MS/MS in female M. rosenbergii, and reveal steroidogenic-related genes by in silico analysis of de novo assembled transcriptomes. Comparative analysis with other species was performed to confirm their putative role, as well as tissue-specific and quantitative gene expression. We reveal 29 transcripts that encode for steroidogenic-related proteins, including steroidogenic enzymes, a nuclear steroid hormone receptors, and a steroidogenic factor. Moreover, we identified for the first time the presence of steroidogenic factor 1, StAR-related lipid transfer protein, estradiol receptor- and progesterone-like protein in M. rosenbergii. Those targeted for gene expression analysis (3 beta-hydroxysteroid dehydrogenase, 17 beta-hydroxysteroid dehydrogenase, estrogen sulfotransferase and progesterone receptor-like) showed widespread expression within many tissues, and at relatively high levels in the central nervous system (CNS) during ovarian maturation. In summary, we provide further evidence for the existence of steroidogenic pathways in crustaceans, which may be useful for advancing prawn aquaculture.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Tipsuda
authors.initials: T
authors.lastname: Thongbuakaew
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand.
authors.forename: Tanapan
authors.initials: T
authors.lastname: Siangcham
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland 4558, Australia.
authors.forename: Saowaros
authors.initials: S
authors.lastname: Suwansa-ard
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland 4558, Australia.
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland 4558, Australia.
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Electronic address: prapee.sre@mahidol.ac.th.
authors.forename: Prapee
authors.initials: P
authors.lastname: Sretarugsa
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000096:biosynthesis*; D004958:Estradiol / Q000096:biosynthesis / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D033541:Palaemonidae / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis / Q000494:pharmacology
chemical_list: D060829:Arthropod Proteins; D011374:Progesterone; D004958:Estradiol
keywords: Aquaculture; Macrobrachium rosenbergii; Steroidogenesis; Transcriptome