title: Visual multiple recognition of protein biomarkers based on an array of aptamer modified gold nanoparticles in biocomputing to strip biosensor logic operations.
abstract: We developed a strip biosensors array based on aptamer-modified gold nanoparticles as receptors and combined the protein-aptamer binding reaction with the streptavidin-biotin interaction as well as the sandwich format. We found that a series of protein receptors obtained a distinct response pattern to each target protein. Three proteins have been well distinguished with the naked eyes and a portable reader without mutual interference, accompanying with lower limit of detection and wider linear range. A complete set of four elementary logic gates (AND, OR, INH, and NAND) and eight combinative logic gates (AND-OR; AND-INH; OR-INH; INH-NAND; AND-OR-INH; AND-INH-NAND; OR-INH-NAND; AND-OR-INH-NAND) are thoroughly realized using this array, which could eventually be applicable to the keypad-lock system with enhanced complexity in the near future. Moreover, this array shows excellent linear relationships, anti-interference capability, real human serum samples applicability, long-term storage stability and reproducibility. All indicate that this design has very good prospects for development.
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Qin
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wen
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. Electronic address: wangsf@hubu.edu.cn.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D001710:Biotin / Q000737:chemistry; D039301:Computers, Molecular; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D019809:Streptavidin / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D011506:Proteins; D001710:Biotin; D006046:Gold; D019809:Streptavidin
keywords: Aptamers; Gold nanoparticles; Logic gate; Protein; The strip biosensors array