title: Identifying the genes regulated by IDH1 via gene-chip in glioma cell U87.
abstract: Glioma is the most common form of primary brain tumor. Increasing evidence show that IDH1 gene mutation is implicated in glioma. However, the mechanism involved in the progression of glioma remains unclear until now. In the study reported here, we used gene chip to identifying the genes regulated with IDH mutanted at R132. The results showed that IDH1-mutant leads to 1255 up-regulated genes and 1862 down-regulated genes in U87 cell lines. Meanwhile, GO and gene-network was performed and shown IDH1-mutant mainly affect small molecule metabolic process, mitotic cell cycle and apoptosis. This result will lay a foundation for further study of IDH1 gene function in the future.
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Lou
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, China.
authors.forename: Daming
authors.initials: D
authors.lastname: Cui
keywords: Glioma; IDH1; gene mutation; gene-chip; overexpression