title: Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates.
abstract: A highly sensitive surface plasmon resonance (SPR) immunosensor for the important ErbB2 breast cancer biomarker has been developed. Optimization of the assay has been carried out, including signal enhancement employing gold nanoparticles (GNPs). The effect of the signal amplification of the GNPs has been also studied. The assay has been tested with clinically relevant matrices. Results in 50% human serum yielded a LOD of 180 pg mL(-1) which is a concentration 83 times lower than the clinical cut-off. Raw lysates from model breast cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-436) have been also assayed and higher quantities of the ErbB2 protein were clearly observed in the ErbB2 over-expressing case (SK-BR-3). The results confirmed that the simple and highly sensitive SPR immunosensor represents a feasible tool for ErbB2 detection.
authors.affiliation: Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain.
authors.forename: Unai
authors.initials: U
authors.lastname: Eletxigerra
authors.affiliation: Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain; Departamento de Física Aplicada 1, Universidad del País Vasco (UPV/EHU), Vitoria, Spain. Electronic address: josu.martinez@tekniker.es.
authors.forename: Josu
authors.initials: J
authors.lastname: Martinez-Perdiguero
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.affiliation: Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain.
authors.forename: Santos
authors.initials: S
authors.lastname: Merino
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: Biosensor; Cell lysates; ErbB2 biomarker; Gold nanoparticles; Serum; Surface plasmon resonance (SPR)