title: Converting cell fates: generating hematopoietic stem cells de novo via transcription factor reprogramming.
abstract: Even though all paradigms of stem cell therapy and regenerative medicine emerged from the study of hematopoietic stem cells (HSCs), the inability to generate these cells de novo or expand them in vitro persists. Initial efforts to obtain these cells began with the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technologies, but these strategies have yet to yield fully functional cells. Subsequently, more recent approaches involve transcription factor (TF) overexpression to reprogram PSCs and various somatic cells. The induction of pluripotency with just four TFs by Yamanaka informs our ability to convert cell fates and demonstrates the feasibility of utilizing terminally differentiated cells to generate cells with multilineage potential. In this review, we discuss the recent efforts undertaken using TF-based reprogramming strategies to convert several cell types into HSCs.
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, New York.|Black Family Stem Cell Institute, Icahn School of Medicine, New York, New York.|The Graduate School of Biomedical Science, Icahn School of Medicine, New York, New York.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Daniel
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, New York.|Black Family Stem Cell Institute, Icahn School of Medicine, New York, New York.|Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine, New York, New York.
authors.forename: Ihor R
authors.initials: IR
authors.lastname: Lemischka
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, New York.|Black Family Stem Cell Institute, Icahn School of Medicine, New York, New York.
authors.forename: Kateri
authors.initials: K
authors.lastname: Moore
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D044968:Regenerative Medicine; D033581:Stem Cell Transplantation / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors
keywords: cell fate conversion; hematopoiesis; hematopoietic stem cells; reprogramming; transcription factors