title: TGF-β1 levels and intraocular tissue alterations in mice infected with a virulent type I RH Toxoplasma gondii strain.
abstract: Toxoplasmosis is generally self-limiting in healthy adults but it may cause toxoplasmic retinochoroiditis in cases of congenital infection leading to blindness. The importance of host genetics in determining disease severity in ocular toxoplasmosis has been shown in different inbred mouse strains using low-virulence toxoplasma strain. In this study, we studied intraocular immune response and tissue alterations in the genetically resistant BALB/c and susceptible MF1 mice infected with a virulent type I RH Toxoplasma gondii strain by intravitreal route. We observed a significant up-regulation of IFN-γ and TNF-α to >2200 pg/ml and >300 pg/ml respectively in the blood of both BALB/c and MF1mice during the early stages of post intraocular infection (p < 0.01) but the levels dropped sharply to normal during the late stages of the infection on day 26. The cytokine levels detected were higher in the MF1 mice compared with the BALB/c mice and a relatively higher levels were observed in the aqueous humour (AqH) than in the blood of both group of mice. The TGF-β1 level in the blood and AqH of BALB/c mice remained low throughout the infection period compared with MF1 mice which showed gradual increase to 50 pg/ml in the blood and AqH during the early stages of infection which then further increased 2-fold-132 pg/ml on day 11 (p < 0.01) and remained high till the last day of observation on day 26 except that the TGF-β1 level in AqH dropped sharply to normal level. In summary, our results support that TGF-β1 may down-regulate the effector functions of anti-Toxoplasma cellular immunity during acute toxoplasmosis. We document that a mild Th1 pro-inflammatory response in the BALB/c mice with high IFN-γ and TNF-α and, low TGF-β1 levels during the early stages of infection may have contributed to an effective cellular immune response leading to lower morbidity, mortality and less ocular tissue damage. However in the MF1 mice, a significantly high TGF-β1 level in the blood as well as in the AqH during the acute intra-ocular toxoplasma infection may have adversely interfered with an effective cellular immune response leading to an increased mortality and extensive ocular tissue damage with parasite tachyzoites observed in the pigment epithelium layers.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box: 24923, Safat 13110, Kuwait. Electronic address: Iqbal@hsc.edu.kw.
authors.forename: Jamshaid
authors.initials: J
authors.lastname: Iqbal
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box: 24923, Safat 13110, Kuwait.
authors.forename: Mohammad Ahmed
authors.initials: MA
authors.lastname: Al-Awadhi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box: 24923, Safat 13110, Kuwait.
authors.forename: Raj Gopal
authors.initials: RG
authors.lastname: Raghupathy
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D016207:Cytokines / Q000032:analysis / Q000097:blood; D015536:Down-Regulation; D005123:Eye / Q000473:pathology; D005260:Female; D007111:Immunity, Cellular; D007249:Inflammation; D051379:Mice; D008807:Mice, Inbred BALB C; D014122:Toxoplasma / Q000472:pathogenicity*; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D014126:Toxoplasmosis, Ocular / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D014774:Virulence
chemical_list: D016207:Cytokines; D053773:Transforming Growth Factor beta1
keywords: Ocular toxoplasmosis; Retinochoroiditis; TGF-β1