title: Metabolic modeling of clostridia: current developments and applications.
abstract: Anaerobic Clostridium spp. is an important bioproduction microbial genus that can produce solvents and utilize a broad spectrum of substrates including cellulose and syngas. Genome-scale metabolic (GSM) models are increasingly being put forth for various clostridial strains to explore their respective metabolic capabilities and suitability for various bioconversions. In this study, we have selected representative GSM models for six different clostridia (Clostridium acetobutylicum, C. beijerinckii, C. butyricum, C. cellulolyticum, C. ljungdahlii and C. thermocellum) and performed a detailed model comparison contrasting their metabolic repertoire. We also discuss various applications of these GSM models to guide metabolic engineering interventions as well as assessing cellular physiology.
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802-1503, USA.
authors.forename: Satyakam
authors.initials: S
authors.lastname: Dash
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802-1503, USA.
authors.forename: Chiam Yu
authors.initials: CY
authors.lastname: Ng
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802-1503, USA costas@engr.psu.edu.
authors.forename: Costas D
authors.initials: CD
authors.lastname: Maranas
mesh_terms: D002482:Cellulose / Q000378:metabolism; D003013:Clostridium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000378:metabolism; D005285:Fermentation; D016680:Genome, Bacterial; D060847:Metabolic Engineering; D008954:Models, Biological
chemical_list: D002482:Cellulose
keywords: Wood-Ljungdahl pathway; cellulotytic; clostridia; genome scale metabolic modeling; solventogenic