title: Real-Time Capture and Visualization of Individual Viruses in Complex Media.
abstract: Label-free imaging of individual viruses and nanoparticles directly in complex solutions is important for virology research and biosensing applications. A successful visualization technique should be rapid, sensitive, and inexpensive, while needing minimal sample preparation or user expertise. Current approaches typically require fluorescent labeling or the use of an electron microscope, which are expensive and time-consuming to use. We have developed an imaging technique for real-time, sensitive, and label-free visualization of viruses and nanoparticles directly in complex solutions such as serum. By combining the advantages of a single-particle reflectance imaging sensor, with microfluidics, we perform real-time digital detection of individual 100 nm vesicular stomatitis viruses as they bind to an antibody microarray. Using this approach, we have shown capture and visualization of a recombinant vesicular stomatitis virus Ebola model (rVSV-ZEBOV) at 100 PFU/mL in undiluted fetal bovine serum in less than 30 min.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Scherr
authors.affiliation: nanoView Diagnostics Inc. , Boston, Massachusetts 02215, United States.
authors.forename: George G
authors.initials: GG
authors.lastname: Daaboul
authors.forename: Jacob
authors.initials: J
authors.lastname: Trueb
authors.forename: Derin
authors.initials: D
authors.lastname: Sevenler
authors.forename: Helen
authors.initials: H
authors.lastname: Fawcett
authors.forename: Bennett
authors.initials: B
authors.lastname: Goldberg
authors.affiliation: Microbiology Department, Boston University School of Medicine , Boston, Massachusetts 02118, United States.
authors.forename: John H
authors.initials: JH
authors.lastname: Connor
authors.forename: M Selim
authors.initials: MS
authors.lastname: Ünlü
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D029043:Ebolavirus / Q000235:genetics; D007118:Immunoassay / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000379:methods; D011994:Recombinant Proteins / Q000276:immunology; D044967:Serum / Q000737:chemistry; D018116:Vesiculovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D055910:Antibodies, Immobilized; D011994:Recombinant Proteins
keywords: Ebola; biosensor; interferometry; label-free; single virus