title: DL-3-n-butylphthalide delays the onset and progression of diabetic cataract by inhibiting oxidative stress in rat diabetic model.
abstract: DL-3-n-butylphthalide (NBP) is a therapeutic drug used for ischemic stroke treatment. Here, we investigated the impact of NBP on the development of rat diabetic cataract induced by intraperitoneal injection of streptozotocin (STZ). NBP was then administrated by oral gavage for nine weeks. Cataract development was monitored through ophthalmoscope inspections. The levels of blood glucose and serum reactive oxygen species (ROS), malondialdehyde (MDA) and 8-Hydroxydeovexyguanosine (8-OHdG) were measured. Total and soluble protein and oxidative stress parameters, such as 2, 4- dinitrophenylhydrazone (DNP), 4-hydroxynonenal (4-HNE) and MDA in the lenses were determined by Western blot and thiobarbituric acid analyses. The expressions of NF-E2-related factor 2 (Nrf2) and its downstream antioxidant enzymes, thioredoxin (TRX), Catalase and nuclear accumulation of Nrf2 were determined by Western blot and immunohistochemistry analyses. We showed that NBP treatment significantly improved the cataract scores, the levels of DNP, 4-HNE, and MDA in the lens compared to the non-treated groups. NBP also enhanced the expressions of Nrf2, TRX and catalase in the lens of diabetic rats. In addition, NBP treatment also decreased levels of blood glucose, serum MDA and 8-OHdG. These results suggested that NBP treatment significantly delayed the onset and progression of diabetic cataract by inhibiting the oxidative stresses.
authors.affiliation: Department of Hematology, the Second Hospital of Hebei Medical University, 215 Western Heping Road, Shijiazhuang 050000, China.
authors.forename: Fuxu
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, the Second Hospital of Hebei Medical University, 215 Western Heping Road, Shijiazhuang 050000, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Digestology, the Second Hospital of Hebei Medical University, 215 Western Heping Road, Shijiazhuang 050000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.affiliation: Department of Ophthalmology, the Second Hospital of Hebei Medical University, 215 Western Heping Road, Shijiazhuang 050000, China.
authors.forename: Xiujin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Laboratorical center for Electron Microscopy, Hebei Medical University, 361 Eastern Zhongshan Road, Shijiazhuang 050017, China.
authors.forename: Li
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Digestology, the Second Hospital of Hebei Medical University, 215 Western Heping Road, Shijiazhuang 050000, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, 361 Eastern Zhongshan Road, Shijiazhuang 050017, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Pathology, Hebei Medical University, 361 Eastern Zhongshan Road, Shijiazhuang 050017, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, 361 Eastern Zhongshan Road, Shijiazhuang 050017, China.|Department of Pathology, Hebei Medical University, 361 Eastern Zhongshan Road, Shijiazhuang 050017, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D015415:Biomarkers; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002386:Cataract / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D048909:Diabetes Complications; D003921:Diabetes Mellitus, Experimental; D004195:Disease Models, Animal; D018450:Disease Progression; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D001572:Benzofurans; D015415:Biomarkers; D001786:Blood Glucose; D051267:NF-E2-Related Factor 2; C495697:Txn1 protein, rat; D013879:Thioredoxins; C027125:3-n-butylphthalide; D002374:Catalase