title: Prevention of amphotericin B nephrotoxicity through use of phytotherapeutic medication.
abstract: Objective To evaluate the effect of diosmin and hesperidin flavonoids in the prevention of amphotericin B nephrotoxicity, through an experimental model on rats. Method Adult, male Wistar rats were distributed into the following groups: saline; diosmin hesperidin (animals that received 50 mg/kg of diosmin hesperidin, drinking water, for ten days); amphotericin B (animals that received 15 mg/kg/day of amphotericin B through intraperitoneal treatment for five days); amphotericin B+diosmin hesperidin. Renal function, fractional excretion of sodium; potassium and magnesium and oxidative metabolites were evaluated. Results Treatment with amphotericin B reduced renal function, as shown by the clearance of creatinine, increased tubular function markers and fractional excretion of sodium, potassium, magnesium and oxidative metabolites. Pre-treatment with diosmin hesperidin ameliorated clearance of creatinine and reduced tubular and oxidative injury. Conclusion Administration of amphotericin B resulted in reduction of renal function with tubular injury, and diosmin hesperidin showing an antioxidant protective effect on the kidneys.
authors.affiliation: Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Fábio Dos Santos
authors.initials: Fdos S
authors.lastname: Schlottfeldt
authors.affiliation: Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Sheila Marques
authors.initials: SM
authors.lastname: Fernandes
authors.affiliation: Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Daniel Malisani
authors.initials: DM
authors.lastname: Martins
authors.affiliation: Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Cordeiro
authors.affiliation: Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Cassiane Dezoti da
authors.initials: CD
authors.lastname: Fonseca
authors.affiliation: Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Mirian
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Departamento de Enfermagem Médico Cirúrgica, Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Maria de Fatima Fernandes
authors.initials: Mde F
authors.lastname: Vattimo