title: Expression and significance of squalene epoxidase in squamous lung cancerous tissues and pericarcinoma tissues.
abstract: BACKGROUND
  A high expression of squalene epoxidase (SQLE) is related to tumor occurrence, development, and prognosis in a variety of cancers. In this study, the expression and significance of SQLE was analyzed in patients with squamous lung cancer and pericarcinoma tissues.
  
  
  METHODS
  The SQLE mRNA and protein expression were separately examined by semi-quantitative reverse transcription polymerase chain reaction, fluorescence quantitative polymerase chain reaction and Western blot in 65 cases of squamous cell lung carcinoma tissues and adjacent non-cancerous lung tissues.
  
  
  RESULTS
  The expression of SQLE mRNA and protein in lung squamous cancerous tissues was significantly higher than in pericarcinoma tissues (63.07% vs. 44.61%, P = 0.0348; 67.69% vs. 38.46%, P = 0.0008). The positive expression rate of SQLE mRNA was not associated with gender, age, smoking, or tumor size (P > 0.05). The expression of SQLE mRNA was closely correlated with poor differentiation, clinical stages, and lymphatic metastasis (P < 0.05). The expression of SQLE mRNA was negatively associated with overall survival rate (P < 0.05).
  
  
  CONCLUSIONS
  A high expression of SQLE was found in squamous lung cancer tissues. The high expression of the SQLE gene may be closely related to the occurrence and development of squamous cell lung carcinoma. SQLE expression predicts a poor prognosis and may serve as a novel lung carcinoma molecule marker.
authors.affiliation: Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, Shandong, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, Shandong, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.affiliation: Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, Shandong, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, Shandong, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Yu
authors.affiliation: Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, Shandong, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Guo
keywords: Lung neoplasms; RT-PCR; SQLE; Western blot