title: Fabrication of duck's feet collagen-silk hybrid biomaterial for tissue engineering.
abstract: Collagen constituting the extracellular matrix has been widely used as biocompatible material for human use. In this study, we have selected duck's feet for extracting collagen. A simple method not utilizing harsh chemical had been employed to extract collagen from duck's feet. We fabricated duck's feet collagen/silk hybrid scaffold for the purpose of modifying the degradation rate of duck's feet collagen. This study suggests that extracted collagen from duck's feet is biocompatible and resembles collagen extracted from porcine which is commercially used. Duck's feet collagen is also economically feasible and it could therefore be a good candidate as a tissue engineering material. Further, addition of silk to fabricate a duck's feet collagen/silk hybrid scaffold could enhance the biostability of duck's feet collagen scaffold. Duck's feet collagen/silk scaffold increased the cell viability compared to silk alone. Animal studies also showed that duck's feet collagen/silk scaffold was more biocompatible than silk alone and more biostable than duck's feet or porcine collagen alone. Additionally, the results revealed that duck's feet collagen/silk hybrid scaffold had high porosity, cell infiltration and proliferation. We suggest that duck's feet collagen/silk hybrid scaffold could be used as a dermal substitution for full thickness skin defects.
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea; Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Hae Sang
authors.initials: HS
authors.lastname: Park
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Ok Joo
authors.initials: OJ
authors.lastname: Lee
authors.affiliation: School of Medicine, George Washington University, Washington D.C. 20037, USA.
authors.forename: Janet Ren
authors.initials: JR
authors.lastname: Chao
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Ju
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Moon
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea.
authors.forename: Ye Ri
authors.initials: YR
authors.lastname: Park
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin, Chonbuk, Jeonju 561 756, Republic of Korea.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Song
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin, Chonbuk, Jeonju 561 756, Republic of Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, School of Medicine, Chuncheon, Republic of Korea; Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University, School of Medicine, Chuncheon, Republic of Korea. Electronic address: hlpch@paran.com.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry*; D004372:Ducks; D005349:Fibroins / Q000737:chemistry; D008297:Male; D051379:Mice; D016062:Porosity; D051381:Rats; D047011:Silk / Q000737:chemistry*; D012844:Sincalide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D003094:Collagen; D005349:Fibroins; D012844:Sincalide
keywords: Collagen; Duck’s feet; Silk