title: Extraction and purification methods in downstream processing of plant-based recombinant proteins.
abstract: During the last two decades, the production of recombinant proteins in plant systems has been receiving increased attention. Currently, proteins are considered as the most important biopharmaceuticals. However, high costs and problems with scaling up the purification and isolation processes make the production of plant-based recombinant proteins a challenging task. This paper presents a summary of the information regarding the downstream processing in plant systems and provides a comprehensible overview of its key steps, such as extraction and purification. To highlight the recent progress, mainly new developments in the downstream technology have been chosen. Furthermore, besides most popular techniques, alternative methods have been described.
authors.affiliation: Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Banacha Street 12/16, 90-237 Lodz, Poland. Electronic address: eweloj@biol.uni.Lodz.pl.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Łojewska
authors.affiliation: Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Banacha Street 12/16, 90-237 Lodz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kowalczyk
authors.affiliation: Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Banacha Street 12/16, 90-237 Lodz, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Olejniczak
authors.affiliation: Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Banacha Street 12/16, 90-237 Lodz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sakowicz
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002845:Chromatography; D010940:Plant Proteins / Q000302:isolation & purification*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification*
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins
keywords: Downstream processing; Extraction of protein; Plant based platforms; Purification of protein; Recombinant protein