title: Multilocus sequence typing of 102 Burkholderia pseudomallei strains isolated from China.
abstract: The phylogenetic and epidemiological relationships of 102 Burkholderia pseudomallei clinical isolates from different geographical and population sources in China were investigated by multilocus sequence typing (MLST). The MLST data were analysed using the e-BURST algorithm, and an unweighted pair-group method with arithmetic mean dendrogram was constructed based on the pair-wise differences in the allelic profiles of the strains. Forty-one sequence types (STs) were identified, of which eight were novel (ST1341, ST1345, ST1346, ST1347, ST1348, ST1349, ST1350, ST1351). No geographical-specific or host population-specific phylogenetic lineages were identified. ST46, ST50, ST55, ST58, ST70 and ST1095 predominated, but ~44% of isolates were assigned to 45 STs illustrating high genetic diversity in the strain collection. Additionally, the phylogenetic relationships of the dominant STs in China showed significant linkeage with B. pseudomallei isolates from Thailand. Analysis of the gmhD allele suggests high genetic variation in B. pseudomallei in China.
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: P
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory,People's Hospital of Sanya City,Hainan Province,People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory,People's Hospital of Sanya City,Hainan Province,People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: C
authors.initials: C
authors.lastname: Ren
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health,Jiangsu Provincial Centre for Disease Control and Prevention,Nanjing,People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Mao
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008554:Melioidosis / Q000453:epidemiology* / Q000382:microbiology*; D008875:Middle Aged; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D010802:Phylogeny
keywords: Burkholderia pseudomallei; e-BURST; multilocus sequence typing (MLST)