title: Multi-substrate biodegradation interaction of 1, 4-dioxane and BTEX mixtures by Acinetobacter baumannii DD1.
abstract: This study evaluated substrate interactions during the aerobic biodegradation of 1, 4-dioxane and BTEX mixtures by a pure culture, Acinetobacter baumannii DD1, which is capable of utilizing 1, 4-dioxane for growth. A. baumannii DD1 could utilize BTEX as a sole carbon source, but could not utilize m-xylene and p-xylene. In binary mixtures, there was a lag of about 14 h before the degradation of BTE, and 1, 4-dioxane only started to be utilized when BTE was completely degraded by 1, 4-dioxane-grown DD1. Furthermore, the biodegradation rate of 1, 4-dioxane decreased from 73.33 to 40.74 mg/(h g dry weight) after the biodegradation of benzene. 1, 4-dioxane could not be degraded after the biodegradation of o-xylene in 80 h. DD1 could also not degrade m-xylene and p-xylene coexisting with 1, 4-dioxane. The ability of DD1 to degrade BTEX occurred in the following order: benzene > ethylbenzene > toluene > o-xylene > m-xylene = p-xylene. The biodegradation of 1, 4-dioxane was not activated in the mixture with o-xylene, primarily because of the accumulation of the specific toxic intermediate, 2, 3-dimethylphenol. The lag in BTE degradation was presumably because of the induction of enzymes necessary for BTE degradation. Additionally, SDS-PAGE analysis demonstrated that there were different proteins during the degradation of benzene and 1, 4-dioxane.
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China.|Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou, 310012, China.
authors.forename: YuYang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China.|Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou, 310012, China.
authors.forename: Huanlin
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China. shends@zju.edu.cn.|Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou, 310012, China. shends@zju.edu.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Shen
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology / Q000378:metabolism*; D001554:Benzene / Q000378:metabolism; D001555:Benzene Derivatives / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004146:Dioxanes / Q000378:metabolism*; D049308:Dioxygenases / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D004146:Dioxanes; D014992:Xylenes; D010105:Oxygenases; D049308:Dioxygenases; D001554:Benzene; C025223:1,4-dioxane
keywords: 1, 4-dioxane; BTEX; Biodegradation; Enzyme; Substrate inhibition