title: Surface Plasmon Resonance Imaging-MALDI-TOF Imaging Mass Spectrometry of Thin Tissue Sections.
abstract: Identification and quantification of proteins in imaging of biological samples are a challenge in today's science. Here, we demonstrate a novel surface plasmon resonance imaging-matrix assisted laser desorption ionization imaging mass spectrometry (SPRi-MALDI IMS) coupled technique competent for the acquisition of multiparametric information by creating a tissue section imprint on an SPRi sensor surface. Correlated images were acquired in SPRi and in MALDI IMS for abundant proteins from a single mouse kidney tissue. The spatial organization of the transferred proteins from the tissue to the SPRi surface was preserved and imaged by SPR and MALDI MS. Surface chemistry was selected to nonspecifically adsorb and retain high concentrations of proteins on the SPRi surface. The diffusion kinetics were controlled to ensure fast transfer of proteins from the tissue sections with minimal lateral diffusion to achieve high spatial fidelity transfer. Lastly, the SPRi instrument was modified to insert a tissue sample in the fluidics chamber to facilitate the real-time measurement of the transfer process. The MALDI IMS experimental conditions, such as matrix deposition and the interface between the SPRi prism and the MALDI IMS instrument, were also optimized. The results show quantitative and regioselective SPRi images correlating to MALDI IMS images of different proteins transferred from a single tissue section.
authors.affiliation: Département de Chimie, Université de Montréal , C.P. 6128 Succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Simon
authors.initials: S
authors.lastname: Forest
authors.affiliation: Département de Chimie, Université de Montréal , C.P. 6128 Succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Julien
authors.initials: J
authors.lastname: Breault-Turcot
authors.affiliation: Département de Chimie, Université de Montréal , C.P. 6128 Succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaurand
authors.affiliation: Département de Chimie, Université de Montréal , C.P. 6128 Succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.|Centre for Self-Assembled Chemical Structures (CSACS), McGill University , Montreal, Quebec H3A 2K6, Canada.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Masson
mesh_terms: D000818:Animals; D004058:Diffusion; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance
chemical_list: D011506:Proteins