title: Genotyping of feline leukemia virus in Mexican housecats.
abstract: Feline leukemia virus (FeLV) is a retrovirus with variable rates of infection globally. DNA was obtained from cats' peripheral blood mononuclear cells, and proviral DNA of pol and env genes was detected using PCR. Seventy-six percent of cats scored positive for FeLV using env-PCR; and 54 %, by pol-PCR. Phylogenetic analysis of both regions identified sequences that correspond to a group that includes endogenous retroviruses. They form an independent branch and, therefore, a new group of endogenous viruses. Cat gender, age, outdoor access, and cohabitation with other cats were found to be significant risk factors associated with the disease. This strongly suggests that these FeLV genotypes are widely distributed in the studied feline population in Mexico.
authors.affiliation: Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Campus 4, Cuautitlán Izcalli Estado de México, Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, CP. 54714, Cuautitlán Izcalli, Estado de México, México. ramiralh@unam.mx.
authors.forename: Hugo
authors.identifier: http://orcid.org/0000-0003-1682-8104
authors.initials: H
authors.lastname: Ramírez
authors.affiliation: Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Campus 4, Cuautitlán Izcalli Estado de México, Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, CP. 54714, Cuautitlán Izcalli, Estado de México, México.
authors.forename: Marcela
authors.initials: M
authors.lastname: Autran
authors.affiliation: Immuno-Virology Laboratory, Department of Immunological Research, UMAE Pediatrics Hospital, XXI Century National Medical Center, IMSS, Av. Cuauhtémoc 330, Col. Doctores, CP. 06725, Ciudad de México, México.
authors.forename: M Martha
authors.initials: MM
authors.lastname: García
authors.affiliation: Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Campus 4, Cuautitlán Izcalli Estado de México, Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, CP. 54714, Cuautitlán Izcalli, Estado de México, México.
authors.forename: M Ángel
authors.initials: MÁ
authors.lastname: Carmona
authors.affiliation: Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Campus 4, Cuautitlán Izcalli Estado de México, Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, CP. 54714, Cuautitlán Izcalli, Estado de México, México.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Rodríguez
authors.affiliation: Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Campus 4, Cuautitlán Izcalli Estado de México, Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, CP. 54714, Cuautitlán Izcalli, Estado de México, México.
authors.forename: H Alejandro
authors.initials: HA
authors.lastname: Martínez
mesh_terms: D000818:Animals; D002415:Cats; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D005256:Leukemia Virus, Feline / Q000235:genetics*; D008297:Male; D008800:Mexico / Q000453:epidemiology; D010802:Phylogeny; D012192:Retroviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012307:Risk Factors; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D004279:DNA, Viral
keywords: Central Mexico; FeLV; PCR; Phylogenetic analysis; Risk factors