title: MicroRNA-503 represses epithelial-mesenchymal transition and inhibits metastasis of osteosarcoma by targeting c-myb.
abstract: Deregulated expression of miRNAs contributes to the development of osteosarcoma. Our previous study has showed that miR-503 was downregulated in osteosarcoma tissues. However, the mechanism of the miR-503 in osteosarcoma development still remains largely undefined. In our study, we found that miR-503 overexpression suppressed cell invasion and migration and inhibited epithelial-to-mesenchymal transition (EMT) of MG-63. Furthermore, we identified that c-myb, an oncogene, was a direct target of miR-503. Moreover, overexpression of c-myb could rescue miR-503-suppressed invasion and EMT. The expression of c-myb was upregulated in osteosarcoma cell lines. Therefore, we conclude that high miR-503 expression suppressed osteosarcoma cell mobility and EMT through targeting c-myb, and this may serve as a therapeutic target.
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Xinzhen
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Ming Shui County People's Hospital, Suihua, Heilongjiang Prov., 150001, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Pang
authors.affiliation: Administration Center Hospital of Nongken, Bei'an, Heilongjiang Prov., 150001, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chong
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China.
authors.forename: Shiqian
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, You Zheng St., Nangang District, Harbin, Heilongjiang Prov., 150001, China. fj3q3q@yeah.net.
authors.forename: Ming
authors.initials: M
authors.lastname: Shao
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D035683:MicroRNAs; D020598:Proto-Oncogene Proteins c-myb
keywords: EMT; MicroRNA; Osteosarcoma; c-myb; miR-503