title: Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity.
abstract: A novel fluorescence detection system was developed for DNA methyltransferase (MTase) activity assay based on a target-protected dumbbell molecular probe mediated cascade rolling circle amplification. It showed excellent specificity and sensitivity with a detection limit of 0.0024 U mL(-1), and potential application in quantitatively monitoring MTase activity and screening of anticancer drugs.
authors.affiliation: Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, P. R. China. wjiang@sdu.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
mesh_terms: D015254:DNA Modification Methylases / Q000378:metabolism*; D057230:Limit of Detection; D015335:Molecular Probes / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D015254:DNA Modification Methylases