title: Anti-proliferative effect of the extract of Guangzao (Fructus Choerospondiatis) on cultured rat cardiac fibroblasts.
abstract: OBJECTIVE
  To ascertain if total flavonoids of Guangzao (Fructus Choerospondiatis) (TFFC) extracted from Guangzao (Fructus Choerospondiatis) can inhibit angiotensin II-induced proliferation of cardiac fibroblasts (CFs).
  
  
  METHODS
  CFs were cultured by the differential attachment method. A model of cell proliferation was established by stimulation with Ang II. Cardiac fibroblasts growth was determined using a hemocytometer. Cell proliferation was detected by methyl thiazole tetrazolium. Lactate dehydrogenase activity was measured by chemical colorimetric method.
  
  
  RESULTS
  Proliferation of TFFC-treated (25, 50, 100 mg/L) fibroblasts was significantly less than that of cells in the angiotensin II group (P < 0.01), and TFFC inhibited proliferation in a dose-dependent manner. These inhibitory effects were partly blocked by pretreatment with NG-nitro-L-arginine methyl ester (L-NAME) and 1H-[1,2,4]-oxadiazole-[4,3-a]-quinoxalin-1-one (ODQ).
  
  
  CONCLUSIONS
  TFFC inhibited angiotensin II-induced proliferation of cardiac fibroblasts via a mechanism that probably involves activation of the NO-cyclic guanosine monophosphate signaling pathway.
authors.forename: Lei
authors.initials: L
authors.lastname: Jia
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yang
authors.forename: Deli
authors.initials: D
authors.lastname: Zhu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Gao
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wang
authors.forename: Huihui
authors.initials: H
authors.lastname: Xing
authors.forename: Junping
authors.initials: J
authors.lastname: Bao
authors.forename: Liang
authors.initials: L
authors.lastname: Shu
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids