title: A Dendritic Golgi Satellite between ERGIC and Retromer.
abstract: The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows visualization of Golgi-related organelles for live imaging in neurons. We show that pGolt labels a widespread microsecretory Golgi satellite (GS) system that is, in contrast to Golgi outposts, present throughout basal and apical dendrites of all pyramidal neurons. The GS system contains glycosylation machinery and is localized between ERGIC and retromer. Synaptic activity restrains lateral movement of ERGIC, GS, and retromer close to one another, allowing confined processing of secretory cargo. Several synaptic transmembrane proteins pass through and recycle back to the GS system. Thus, the presence of an ER-ERGIC-GS-retromer microsecretory system in all neuronal dendrites enables autonomous local control of transmembrane protein synthesis and processing.
authors.affiliation: RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany; Emmy-Noether Group "Neuronal Protein Transport", University Medical Center Hamburg-Eppendorf, Center for Molecular Neurobiology, ZMNH, 20251 Hamburg, Germany; Cell Biology, Faculty of Science, Utrecht University, 3584 Utrecht, the Netherlands.
authors.forename: Marina
authors.initials: M
authors.lastname: Mikhaylova
authors.affiliation: RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany.
authors.forename: Sujoy
authors.initials: S
authors.lastname: Bera
authors.affiliation: Combinatorial Neuroimaging Core Facility (CNI), Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Kobler
authors.affiliation: Department of Neurochemistry/Molecular Biology, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany.
authors.forename: Renato
authors.initials: R
authors.lastname: Frischknecht
authors.affiliation: RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany; Leibniz Guest Group "Dendritic Organelles and Synaptic Function", University Medical Center Hamburg-Eppendorf, Center for Molecular Neurobiology, ZMNH, 20251 Hamburg, Germany. Electronic address: kreutz@lin-magdeburg.de.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Kreutz
mesh_terms: D003712:Dendrites / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009474:Neurons; D015388:Organelles / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins