title: [Effect of Serum Containing Sesamin on Angiotensin II-Induced Apoptosis in Rat Cardiomyocytes].
abstract: OBJECTIVE
  To investigate the effect of serum containing sesamin on angiotension II (Ang II)-induced apoptosis in rat cardiomyocytes and the possible mechanisms.
  
  
  METHODS
  H9c2 rat cardiomyocytes were preincubated with serum containing sesamin or blank serum for 12 h, followed by incubation with Ang II for 24 h. Cell viability was assessed by MTT assay and cell apoptosis was evaluated by flow cytometric analysis. Protein expression of BCL-2, BAX, Caspase-3, p47phox and superoxide dismutase (SOD) was determined by Western blot analysis. Levels of intracellular reactive oxygen species (ROS), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) were measured colorimetrically.
  
  
  RESULTS
  Preincubation with serum containing sesamin significantly improved cell viability and suppressed cell apoptosis in H9c2 rat cardiomyocytes exposed to Ang II (P < 0.05 or P < 0.01), with the expression of BAX, Caspase-3 and p47phox protein down-regulated and BCL-2 and SOD protein up-regulated markedly (P < 0.05 or P < 0.01). The levels of T-AOC were effectively increased in serum containing sesamin groups, while the levels of intracellular ROS and MDA contents were decreased significantly (P < 0.05 or P < 0.01). Control serum had no influence on the above mentioned measurements.
  
  
  CONCLUSIONS
  Sesamin is capable of suppressing Ang II-induced apoptosis in H9c2 rat cardiomyocytes, which might be derived, at least partly, from amelioration of oxidative stress, regulation of BAX/BCL-2 protein expression and suppression of Caspase-3 protein expression.
authors.forename: Shu-guo
authors.initials: SG
authors.lastname: Zheng
authors.forename: You-nan
authors.initials: YN
authors.lastname: Ren
authors.forename: Meng-qiu
authors.initials: MQ
authors.lastname: Zhao
authors.forename: Shan-jun
authors.initials: SJ
authors.lastname: Tao
authors.forename: Xiang
authors.initials: X
authors.lastname: Kong
authors.forename: Jie-ren
authors.initials: JR
authors.lastname: Yang
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D004149:Dioxoles / Q000494:pharmacology*; D017705:Lignans / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects*; D018384:Oxidative Stress; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; C494828:Bax protein, rat; D004149:Dioxoles; D017705:Lignans; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D000804:Angiotensin II; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C505410:Casp3 protein, rat; D053148:Caspase 3; C054125:sesamin