title: Allelic Variation of BnaC.TT2.a and Its Association with Seed Coat Color and Fatty Acids in Rapeseed (Brassica napus L.).
abstract: Efficient molecular markers for the selection of rapeseed genetic materials with high seed oil content and ideal fatty acid (FA) composition are preferred by rapeseed breeders. Recently, we reported the molecular mechanism of TRANSPARENT TESTA 2 (TT2) in inhibiting seed FA biosynthesis in Arabidopsis. However, evidence showing the association of rapeseed TT2 homologs and seed FA production are still insufficient. In this study, we collected 83 rapeseed (Brassica napus L.) landraces from different geographical backgrounds to conduct association mapping of BnaC.TT2.a in relation to seed coat color and FA biosynthesis. Population background was corrected by 84 pairs of SSR markers that were uniformly distributed among the linkage groups of the Tapidor-Ningyou-7 DH population. A single copy of BnaC.TT2.a for single nucleotide polymorphism (SNP) assay was cloned by a pair of previously reported specific primers. From the analysis of BnaC.TT2.a allelic variations using GLM+Q model, four SNPs on intron 1 of BnaC.TT2.a that were associated with seed FA were discovered. Moreover, an InDel at position 738 on exon 3 of BnaC.TT2.a indicated a change of protein function that was significantly associated with seed coat color, linoleic acid (C18:2), and total FA content. These findings revealed the role of BnaC.TT2.a in regulating the seed color formation and seed FA biosynthesis in rapeseed, thereby suggesting effective molecular markers for rapeseed breeding.
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Nazim
authors.initials: N
authors.lastname: Hussain
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Lixi
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000483:Alleles; D029688:Brassica napus / Q000235:genetics*; D002138:Calibration; D005227:Fatty Acids / Q000378:metabolism*; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D054643:INDEL Mutation / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics; D012639:Seeds / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers; D010940:Plant Proteins