title: Pasteurellaceae bacteria from the oral cavity of Tasmanian devils (Sarcophilus Harrisii) show high minimum inhibitory concentration values towards aminoglycosides and clindamycin.
abstract: Threatened by Devil Facial Tumor Disease, the Tasmanian devil populations are vulnerable and decreasing. Additionally, the devils' biting behaviour elevates their risk of acquiring bite wound infections caused by members of the bacterial Pasteurellaceae family that are natural inhabitants of the oral microbiota. In medical management of such bite wounds, antimicrobial susceptibility profiles are crucial. Prior to this investigation, no available data on minimal inhibitory concentration (MIC) values existed. A total of 26 isolates obtained from the oral cavity of 26 healthy Tasmanian devils were tested for their antimicrobial susceptibility by broth micro dilution. Most prominently, high MIC values for clindamycin (≥4 μg ml(-1) ), gentamicin (≥8 μg ml(-1) ) and amikacin (≥32 μg ml(-1) ), were observed for 92, 77 and 73% of the strains tested respectively. This study may be used as a guideline for antimicrobial therapy against bite wound infections caused by Pasteurellaceae originating from the oral cavity of Tasmanian devils.
  
  
  CONCLUSIONS
  Tasmanian devils' aggressive behaviour makes bite wounds in fellow devils and human caretakers a common entity. Pasteurellaceae bacteria are common inhabitants of the oral microbiota of Tasmanian devils and a likely cause of bite wound infections. Here, for the first time, we report antimicrobial sensitivity profiles from a broad collection of Pasteurellaceae isolates obtained from the oral cavity of Tasmanian devils. Low MIC values were observed for the majority of the 22 antimicrobial agents included, yet nearly all strains were tolerant to clindamycin and the aminoglycosides. The work can serve as a guide for clinicians involved in treatment of bite wounds inflicted by devils in animals and humans.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medicine, University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: N
authors.initials: N
authors.lastname: Gutman
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medicine, University of Copenhagen, Frederiksberg C, Denmark.|Centre for Zoo and Wild Animal Health, Copenhagen Zoo, Frederiksberg, Denmark.|Institute for Marine and Antarctic Studies, University of Tasmania, Launceston, TAS, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hansen
authors.affiliation: Centre for Zoo and Wild Animal Health, Copenhagen Zoo, Frederiksberg, Denmark.
authors.forename: M F
authors.initials: MF
authors.lastname: Bertelsen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medicine, University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: A M
authors.initials: AM
authors.lastname: Bojesen
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001733:Bites and Stings / Q000382:microbiology; D002981:Clindamycin / Q000494:pharmacology*; D005153:Facial Neoplasms; D006801:Humans; D008394:Marsupialia / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D015661:Pasteurellaceae / Q000187:drug effects* / Q000302:isolation & purification; D014946:Wound Infection / Q000382:microbiology
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002981:Clindamycin
keywords: MIC; Pasteurellaceae; Sarcophilus harrisii; Tasmanian devil; antimicrobial susceptibility; minimum inhibitory concentration