title: Phosphoric acid pretreatment of Achyranthes aspera and Sida acuta weed biomass to improve enzymatic hydrolysis.
abstract: Achyranthes aspera and Sida acuta, two types of weed biomass are abundant and waste in Thailand. We focus on them as novel feedstock for bio-ethanol production because they contain high-cellulose content (45.9% and 46.9%, respectively) and unutilized material. Phosphoric acid (70%, 75%, and 80%) was employed for the pretreatment to improve by enzymatic hydrolysis. The pretreatment process removed most of the xylan and a part of the lignin from the weeds, while most of the glucan remained. The cellulose conversion to glucose was greater for pretreated A. aspera (86.2 ± 0.3%) than that of the pretreated S. acuta (82.2 ± 1.1%). Thus, the removal of hemicellulose significantly affected the efficiency of the enzymatic hydrolysis. The scanning electron microscopy images showed the exposed fibrous cellulose on the cell wall surface, and this substantial change of the surface structure contributed to improving the enzyme accessibility.
authors.affiliation: Department of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000, Thailand.
authors.forename: Premjet
authors.initials: P
authors.lastname: Siripong
authors.affiliation: Center for Agricultural Biotechnology, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Muang, Phitsanulok 65000, Thailand.
authors.forename: Premjet
authors.initials: P
authors.lastname: Duangporn
authors.affiliation: Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan; Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Takata
authors.affiliation: Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: y-tsutsu@agr.kyushu-u.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tsutsumi
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D056804:Biofuels; D018533:Biomass; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D003246:Conservation of Energy Resources / Q000379:methods; D005936:Glucans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D019660:Malvaceae / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D013785:Thailand
chemical_list: D056804:Biofuels; D005936:Glucans; D010756:Phosphoric Acids; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; C030242:phosphoric acid; D005947:Glucose
keywords: Concentrated phosphoric acid; Enzymatic hydrolysis; Lignocellulosic feedstocks; Pretreatment; Weed biomass