title: Microwave-Assisted Extraction Followed by Solid-Phase Extraction for the Chromatographic Analysis of Alkaloids in Stephania cepharantha.
abstract: A procedure involving microwave-assisted extraction (MAE) followed by solid-phase extraction (SPE) was established for the extraction and purification of three bisbenzylisoquinoline alkaloids from Stephania cepharantha, and a reversed-phase high-performance liquid chromatography (HPLC) method was developed for the quantification of the target alkaloids. Chromatographic separation was achieved on a Phenomenex Luna Phenyl-Hexyl column. Prior to the HPLC analysis, the alkaloids were rapidly extracted by an optimized MAE process using 0.01 mol/L hydrochloric acid as the solvent. The MAE extract was subsequently purified by SPE using a cation-exchange polymeric cartridge. The MAE-SPE procedure extracted the three alkaloids with satisfactory recoveries ranging from 100.44 to 102.12%. In comparison with the MAE, Soxhlet and ultrasonic-assisted extractions, the proposed MAE-SPE method showed satisfactory cleanup efficiency. Thus, the validated MAE-SPE-HPLC method is specific, accurate and applicable to the determination of alkaloids in S. cepharantha.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Daotao
authors.initials: D
authors.lastname: Xie
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Instrumental Analysis Center, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Jixian
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China jmhuang@shmu.edu.cn.
authors.forename: Jianming
authors.initials: J
authors.lastname: Huang
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008872:Microwaves; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods*; D031607:Stephania / Q000737:chemistry*
chemical_list: D000470:Alkaloids