title: Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway.
abstract: Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23 mM; 288±17.7 mg/L) and glycerol (12.62±1.55 mM; 1.16±0.14 g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.
authors.affiliation: Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hirokawa
authors.affiliation: Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Maki
authors.affiliation: Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Tatsuke
authors.affiliation: Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: taizo@brs.kyushu-u.ac.jp.
authors.forename: Taizo
authors.initials: T
authors.lastname: Hanai
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism*; D000458:Cyanobacteria / Q000502:physiology* / Q000528:radiation effects; D005990:Glycerol / Q000378:metabolism; D008027:Light; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D010788:Photosynthesis / Q000502:physiology* / Q000528:radiation effects; D011409:Propylene Glycols / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D058615:Synthetic Biology / Q000379:methods
chemical_list: D011409:Propylene Glycols; D011994:Recombinant Proteins; D002245:Carbon Dioxide; C041787:1,3-propanediol; D005990:Glycerol
keywords: 1,3-propanediol production; Cyanobacteria; Dihydroxyacetone phosphate; Pseudovitamin B(12); Synthetic metabolic pathway