title: Pharmacokinetic and bioavailability study of angeloylgomisin H in rat plasma by UPLC-MS/MS.
abstract: Angeloylgomisin H, as a major lignin in the fruits, was reported to have the potential to improve insulin-stimulated glucose uptake by activating PPAR-γ. In this work, a sensitive and selective UPLC-MS/MS method for determination of angeloylgomisin H in rat plasma is developed. After addition of rutin as an internal standard (IS), protein precipitation by acetonitrile was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reactions monitoring (MRM) mode was used for quantification using target fragment ions m/z 523.2-315.1 for angeloylgomisin H, and m/z 611.1-303.1 for IS. Calibration plots were linear throughout the range 5-2000 ng/mL for angeloylgomisin H in rat plasma. Mean recoveries of angeloylgomisin H in rat plasma ranged from 86.2% to 92.5%. RSD of intra-day and inter-day precision were both < 11%. The accuracy of the method was between 93.0% and 104.1%. The method was successfully applied to pharmacokinetic study of angeloylgomisin H after either oral or intravenous administration. The absolute bioavailability of angeloylgomisin H was reported as high as 4.9%.
authors.affiliation: Department of Cardiology, Wenzhou Central Hospital Wenzhou 325000, Zhejiang, China.
authors.forename: Shanjiang
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Wenzhou Central Hospital Wenzhou 325000, Zhejiang, China.
authors.forename: Zhuyin
authors.initials: Z
authors.lastname: Jia
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, China.
authors.forename: Ledan
authors.initials: L
authors.lastname: Dong
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui Lishui 323000, Zhejiang, China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Geng
authors.affiliation: Laboratory Animal Centre, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
authors.forename: Zezheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Laboratory Animal Centre, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
authors.forename: Suping
authors.initials: S
authors.lastname: Yang
authors.affiliation: Laboratory Animal Centre, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, China.
authors.forename: Fuli
authors.initials: F
authors.lastname: Liu
keywords: Angeloylgomisin H; UPLC-MS/MS; pharmacokinetics; rat plasma