title: Elevated Levels of Urinary Markers of Oxidative DNA and RNA Damage in Type 2 Diabetes with Complications.
abstract: The mechanisms underlying progression of type 2 diabetes are complex and varied. Recent studies indicated that oxidative stress provided a new sight. To further assess the relationship between nucleic acid oxidation and complications in patients with type 2 diabetes and explore its possible molecular mechanisms, we studied 1316 subjects, including 633 type 2 diabetes patients and 683 age- and sex-matched healthy controls. Urinary levels of DNA oxidation marker 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and RNA oxidation marker 8-oxo-7,8-dihydroguanosine (8-oxoGuo) were measured by ultraperformance liquid chromatography and mass spectrometry (UPLC-MS/MS). Serum glucose, HbA1c, total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides (TG) were also determined. The results showed significantly elevated levels of both the urinary 8-oxodGuo and 8-oxoGuo in diabetes patients with/without complications compared with age-matched healthy control subjects (p = 0.02 and p < 0.001, resp.). Patients with complications, especially macrovascular complications, exhibited higher levels of 8-oxoGuo than those without complications, while there was no difference in the concentrations of serum glucose and lipids. The finding indicates the role for oxidative damage to DNA and RNA, as a molecular mechanism contributing to the progression of type 2 diabetes. Elevated levels of 8-oxoGuo may be a risk factor for type 2 diabetes complications, especially in diabetic macrovascular complications.
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Xinle
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China; The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gan
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Yuangao
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Bin
authors.identifier: 0000-0002-0275-3722
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Lab Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China.
authors.forename: Jianping
authors.identifier: 0000-0003-2793-9221
authors.initials: J
authors.lastname: Cai
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D004247:DNA / Q000652:urine*; D004249:DNA Damage; D003710:Demography; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D048909:Diabetes Complications / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012313:RNA / Q000652:urine*
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine; D012313:RNA; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine