title: Effects of fertilizer application and dry/wet processing of Miscanthus x giganteus on bioethanol production.
abstract: The effects of wet and dry processing of miscanthus on bioethanol production using simultaneous saccharification and fermentation (SSF) process were investigated, with wet samples showing higher ethanol yields than dry samples. Miscanthus grown with no fertilizer, with fertilizer and with swine manure were sampled for analysis. Wet-fractionation was used to separate miscanthus into solid and liquid fractions. Dilute sulfuric acid pretreatment was employed and the SSF process was performed with saccharomyces cerevisiae and a cocktail of enzymes at 35°C. After pretreatment, cellulose compositions of biomass of the wet samples increased from 61.0-67.0% to 77.0-87.0%, which were higher than the compositions of dry samples. The highest theoretical ethanol yield of 88.0% was realized for wet processed pretreated miscanthus, grown with swine manure. Changes to the morphology and chemical composition of the biomass samples after pretreatment, such as crystallinity reduction, were observed using SEM and FTIR. These changes improved ethanol production.
authors.affiliation: Energy and Environmental Systems Program, College of Arts and Science, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States; Biological Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States.
authors.forename: Nana Abayie
authors.initials: NA
authors.lastname: Boakye-Boaten
authors.affiliation: Biological Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States. Electronic address: xshuangn@ncat.edu.
authors.forename: Shuangning
authors.initials: S
authors.lastname: Xiu
authors.affiliation: Biological Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Shahbazi
authors.affiliation: Biological Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Joint School of Nanoscience and Nanoengineering, North Carolina A & T State University, 2907 E. Gate City Blvd, Greensboro, NC 27401, United States; Biological Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Biological Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States.
authors.forename: Michelle
authors.initials: M
authors.lastname: Mims
authors.affiliation: Energy and Environmental Systems Program, College of Arts and Science, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, United States.
authors.forename: Keith
authors.initials: K
authors.lastname: Schimmel
mesh_terms: D000818:Animals; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000378:metabolism; D003246:Conservation of Energy Resources; D000431:Ethanol / Q000032:analysis / Q000737:chemistry; D005285:Fermentation; D005308:Fertilizers; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D008372:Manure; D006109:Poaceae / Q000737:chemistry* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013464:Sulfuric Acids / Q000737:chemistry; D013552:Swine
chemical_list: D056804:Biofuels; D005308:Fertilizers; D008372:Manure; D013464:Sulfuric Acids; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C033158:sulfuric acid
keywords: Bioethanol; Dilute sulfuric acid pretreatment; Miscanthus x giganteus (MxG); Simultaneous saccharification and fermentation; Wet and dry processing