title: Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT.
abstract: The purpose of this study is to explore the role of hypoxia on the invasion and metastasis of laryngeal carcinoma. The invasion and migration ability of laryngeal cancer SCC10A cell was detected by transwell assay. Western blot was applied to analyze the expression of EMT-related proteins. Fifty-seven samples from postoperative patients with laryngeal cancer were collected to study. Immunohistochemistry was used to examine the expression of GLUT-1 and EMT-related proteins (Vim, E-cad, N-cad) in normal laryngeal squamous epithelial tissue, laryngeal cancer adjacent tissues and laryngeal squamous cell carcinoma tissues. Hypoxia promoted laryngeal cancer cell invasion and migration. Hypoxia also enhanced the expression of GLUT-1, vimentin and N-cad, which exist statistically significant correlation with the clinical staging and lymph node metastases (P < 0.05). The expression of GLUT-1 is positively correlated with Vim and N-cad expression in laryngeal squamous cell carcinoma tissues, but negatively correlated with E-cad expression. The patient survival rate with the positive expression of GLUT-1, Vim and N-cad becomes much shorter compared with those with negative expression of GLUT-1, Vim and N-cad (P < 0.05). Hypoxia promoted laryngeal cancer cell invasion and migration via EMT.
authors.affiliation: Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China. jhzuoch@sina.com.|Nanhua Hospital, University of South China, Hengyang, 421000, Hunan, People's Republic of China. jhzuoch@sina.com.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zuo
authors.affiliation: Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhangjiajie City Hospital, Zhangjiajie, 427000, Hunan, People's Republic of China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Lei
authors.affiliation: Nanhua Hospital, University of South China, Hengyang, 421000, Hunan, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Wen
authors.affiliation: Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China.|Nanhua Hospital, University of South China, Hengyang, 421000, Hunan, People's Republic of China.
authors.forename: Sai
authors.initials: S
authors.lastname: Li
authors.affiliation: Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China.|Nanhua Hospital, University of South China, Hengyang, 421000, Hunan, People's Republic of China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Lv
authors.affiliation: Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China. 1637790824@qq.com.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China. gb_wen@yahoo.com.cn.
authors.forename: Gebo
authors.initials: G
authors.lastname: Wen
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C050361:CDH2 protein, human; D015820:Cadherins; D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human; D014746:Vimentin
keywords: E-cadherin; Epithelial–mesenchymal transition; Glucose transporter-1; Laryngeal carcinoma; Vimentin