title: Antileukemic activity of lignans and phenylpropanoids of Cinnamomum parthenoxylon.
abstract: In this study, we evaluated the in vitro cytotoxicity of fractions and isolated constituents from Cinnamomum parthenoxylon woods against human leukemia HL-60 and U937 cells. The n-Hex, EtOAc, and MeOH-H2O fractions of the woods inhibited cell proliferation in both cell lines. Our phytochemical investigation of the n-Hex and EtOAc fractions led to the isolation of lignans and phenylpropanoids, whose chemical structures were confirmed by spectroscopic analyses. All isolated compounds were evaluated for their in vitro antileukemic activity; especially, hinokinin and cubebin exhibited strong inhibition toward U937 cell proliferation. Morphological observation indicated that these cytotoxic actions were mediated by apoptosis. Our findings suggested that an oxygenated functional group at the C-9 position in dibenzylfuran skeleton contributed their potency. In addition, these results enhanced the ethnopharmacological value of C. parthenoxylon.
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bengkulu University, Jalan Raya W.R. Supratman, Kandang Limun, Bengkulu 38371, Indonesia.
authors.forename: Morina
authors.initials: M
authors.lastname: Adfa
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bengkulu University, Jalan Raya W.R. Supratman, Kandang Limun, Bengkulu 38371, Indonesia.
authors.forename: Rizki
authors.initials: R
authors.lastname: Rahmad
authors.affiliation: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ninomiya
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bengkulu University, Jalan Raya W.R. Supratman, Kandang Limun, Bengkulu 38371, Indonesia.
authors.forename: Salprima
authors.initials: S
authors.lastname: Yudha S
authors.affiliation: Division of Anaerobe Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan; United Graduate School of Drug Discovery and Medicinal Information Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: koketsu@gifu-u.ac.jp.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D052117:Benzodioxoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D027424:Cinnamomum / Q000737:chemistry* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D020298:U937 Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D052117:Benzodioxoles; D017705:Lignans; D010936:Plant Extracts; C475934:hinokinin; C433065:cubebin; D015107:4-Butyrolactone
keywords: Antileukemic activity; Apoptosis; Cinnamomum parthenoxylon; Lignan; Phenylpropanoid