title: iBodies: Modular Synthetic Antibody Mimetics Based on Hydrophilic Polymers Decorated with Functional Moieties.
abstract: Antibodies are indispensable tools for biomedicine and anticancer therapy. Nevertheless, their use is compromised by high production costs, limited stability, and difficulty of chemical modification. The design and preparation of synthetic polymer conjugates capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, immunocytochemistry, and immunoprecipitation is reported. The conjugates, named "iBodies", consist of an HPMA copolymer decorated with low-molecular-weight compounds that function as targeting ligands, affinity anchors, and imaging probes. We prepared specific conjugates targeting several proteins with known ligands and used these iBodies for enzyme inhibition, protein isolation, immobilization, quantification, and live-cell imaging. Our data indicate that this highly modular and versatile polymer system can be used to produce inexpensive and stable antibody substitutes directed toward virtually any protein of interest with a known ligand.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Šácha
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Knedlík
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Schimer
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Tykvart
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Parolek
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Navrátil
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Dvořáková
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.|First Faculty of Medicine, Charles University, Kateřinská 32, 12108, Prague 2, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Sedlák
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Heyrovského n. 2, 16206, Prague 6, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Ulbrich
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Heyrovského n. 2, 16206, Prague 6, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Strohalm
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Majer
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Heyrovského n. 2, 16206, Prague 6, Czech Republic. subr@imc.cas.cz.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Šubr
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Flemingovo n. 2, 16610, Prague 6, Czech Republic. konval@uochb.cas.cz.|Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague 2, Czech Republic. konval@uochb.cas.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Konvalinka
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018716:Molecular Mimicry; D011108:Polymers / Q000737:chemistry*
chemical_list: D000906:Antibodies; D011108:Polymers
keywords: HPMA; antibody mimetics; molecular recognition; polymer conjugates; protein targeting