title: Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean.
abstract: Sclerotinia stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary is a major disease of soybean (Glycine max (L.) Merr.). At present, we revealed the three-way interaction between Trichoderma harzianum T-aloe, pathogen S. sclerotiorum and soybean plants in order to demonstrate biocontrol mechanism and evaluate biocontrol potential of T-aloe against S. sclerotiorum in soybean. In our experiments, T-aloe inhibited the growth of S. sclerotiorum with an efficiency of 56.3% in dual culture tests. T-aloe hyphae grew in parallel or intertwined with S. sclerotiorum hyphae and produced hooked contact branches, indicating mycoparasitism. Plate tests showed that T-aloe culture filtrate inhibited S. sclerotiorum growth with an inhibition efficiency of 51.2% and sclerotia production. T-aloe pretreatment showed growth-promoting effect on soybean plants. The activities of peroxidase, superoxide dismutase, and catalase increased, and the hydrogen peroxide (H2O2) as well as the superoxide radical (O2(-)) content in soybean leaves decreased after T-aloe pretreatment in response to S. sclerotiorum pathogen challenge. T-aloe treatment diminished damage caused by pathogen stress on soybean leaf cell membrane, and increased chlorophyll as well as total phenol contents. The defense-related genes PR1, PR2, and PR3 were expressed in the leaves of T-aloe-treated plants. In summary, T-aloe displayed biocontrol potential against S. sclerotiorum. This is the first report of unraveling biocontrol potential of Trichoderma Spp. to soybean sclerotinia stem rot from the three-way interaction between the biocontrol agent, pathogen S. sclerotiorum and soybean plants.
authors.affiliation: Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences, Xinjiang 830011, China; College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China. Electronic address: zhangfl2007@126.com.
authors.forename: Fuli
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Ge
authors.affiliation: College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
authors.affiliation: Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences, Xinjiang 830011, China; School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China.
authors.forename: Xiue
authors.initials: X
authors.lastname: Ji
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, College of Life Science and Agronomy, ZhouKou Normal University, ZhouKou 466001, China. Electronic address: lichengwei@zknu.edu.cn.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Li
mesh_terms: D001203:Ascomycota; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D013025:Glycine max / Q000382:microbiology*; D014242:Trichoderma / Q000254:growth & development*
keywords: Resistance; SA and JA/ET signaling; Sclerotinia sclerotiorum; Soybean seedlings; Trichoderma harzianum T-aloe