title: Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu²⁺ and Biological Thiols in Aqueous Solution and Living Cells.
abstract: A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu(2+) and biothiols. It was found that HNA can specifically bind to Cu(2+) with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu(2+) ensemble displayed a "turn-on" fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu(2+) to Hcy, Cys and GSH were estimated to be 1.5 μM, 1.0 μM and 0.8 μM, respectively, suggesting that HNA-Cu(2+) is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu(2+) to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay.
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China. jhongmin66@163.com.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Jia
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China. yangming0721@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China. qtmeng@ustl.edu.cn.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, He'nan 453003, China. guangjiehe@163.com.
authors.forename: Guangjie
authors.initials: G
authors.lastname: He
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China. Wangyue9088@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China. huzhizhi@163.com.
authors.forename: Zhizhi
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. run.zhang@mq.edu.au.
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China. zhangzhiqiang@ustl.edu.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D003300:Copper / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D016014:Linear Models; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013881:Thioridazine / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D003300:Copper; D013881:Thioridazine
keywords: aldazine; biothiols; chemosensing ensemble; detection; fluorescence imaging