title: Bacteroides caecicolasp. nov. andBacteroides gallinaceumsp. nov., isolated from the caecum of an Indonesian chicken.
abstract: Six strains of anaerobic bacteria, C13EG70T, C13EG118, C13EG186T, C13GAMG5, C13GAMG28 and C13GAMG40, were isolated from the caecum of a healthy chicken bred in Bogor, Indonesia. Phylogenetic analysis showed the isolates were separated into two groups. Group I (C13EG70Tand C13EG118) showed nearly identical 16S rRNA gene sequences (99.9 % sequence similarity). Group II (C13EG186T, C13GAMG5, C13GAMG28 and C13GAMG40) showed nearly identical 16S rRNA gene sequences (>99.4 % sequence similarity). The isolates showed low 16S rRNA gene sequence similarities to recognized species of the genusBacteroides. High gene sequence similarities were found between type strains (C13EG70Tand C13EG186T) andBacteroides salanitronisJCM 13657T(87.9, 91.5 %, respectively). Physiological, biochemical and genotypic characteristics demonstrated that these strains could be separated from the type strain ofB. salanitronis. It is concluded that Group I and Group II represent novel species. Two novel species of the genusBacteroidesare proposed asBacteroides caecicolasp. nov. (type strain C13EG70T= LIPI12-4-Ck732T= JSAT12-4-Ck732T= InaCC B449T= NBRC 110958T) andBacteroides gallinaceumsp. nov. (type strain C13EG186T= LIPI12-4-Ck844T= JSAT12-4-Ck884T= InaCC B451T= NBRC 110963T).
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Irisawa
authors.affiliation: Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta-Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Sugiyono
authors.initials: S
authors.lastname: Saputra
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kitahara
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta-Bogor Km. 46, Cibinong 16911, Indonesia.
authors.lastname: Sulistiani
authors.affiliation: Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta-Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Titin
authors.initials: T
authors.lastname: Yulineri
authors.affiliation: Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta-Bogor Km. 46, Cibinong 16911, Indonesia.
authors.forename: Achmad
authors.initials: A
authors.lastname: Dinoto
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma