title: Pseudahrensia todarodissp. nov., isolated from the gut of a Japanese flying squid,Todarodes pacificus.
abstract: A novel Gram-stain-negative, non-motile, non-spore-forming, non-flagellated, aerobic, beige-coloured and rod-shaped bacterium, designated strain KHS02T, was isolated from the intestinal tract of a Japanese flying squid,Todarodes pacificus, which was collected from the East Sea, Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain KHS02Tformed a monophyletic clade withPseudahrensia aquimarisHDW-32T, with which it had the highest sequence similarity (98.67 %). Strain KHS02Tgrew optimally at pH 7 with 2 % (w/v) NaCl at 25 °C on marine broth 2216, but could not grow without Na+. The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids (>10 % of the total) were summed feature 8 (C18 : 1ω6cand/or C18 : 1ω7c) and 11-methyl C18 : 1ω7c. The polar lipids of strain KHS02Tcomprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and an unidentified lipid. The genomic DNA G+C content was 58.6 mol%. DNA-DNA hybridization showed that the isolate shared 16.2 ± 1.3 % (reciprocal, 15.7 ± 2.8 %) genomic relatedness with the type strain of the closest species. In conclusion, this isolate is suggested to represent a novel species of the genusPseudahrensia, for which the namePseudahrensia todarodisis proposed. The type strain is KHS02T( = KACC 18257T= JCM 30419T).
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Hyun Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Pil Soo
authors.initials: PS
authors.lastname: Kim
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: June-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Woorim
authors.initials: W
authors.lastname: Kang
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Na-Ri
authors.initials: NR
authors.lastname: Shin
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Tae Woong
authors.initials: TW
authors.lastname: Whon
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae