title: Caffeine treatment aggravates secondary degeneration after spinal cord injury.
abstract: Spinal cord injury (SCI) often results in some form of paralysis. Recently, SCI therapy has been focused on preventing secondary injury to reduce both neuroinflammation and lesion size so that functional outcome after an SCI may be improved. Previous studies have shown that adenosine receptors (AR) are a major regulator of inflammation after an SCI. The current study was performed to examine the effect of caffeine, a pan-AR blocker, on spontaneous functional recovery after an SCI. Animals were assigned into 3 groups randomly, including sham, PBS and caffeine groups. The rat SCI was generated by an NYU impactor with a 10 g rod dropped from a 25 mm height at thoracic 9 spinal cord level. Caffeine and PBS were injected daily during the experiment period. Hind limb motor function was evaluated by the Basso, Beattie, Bresnahan (BBB) locomotor rating scale at 1 week and 4 weeks after the SCI. Spinal cord segments were collected after final behavior evaluation for morphological analysis. The tissue sparing was evaluated by luxol fast blue staining. Immunofluorescence stain was employed to assess astrocyte activation and neurofilament positioning, while microglia activation was examined by immunohistochemistry stain.The results showed that spontaneous functional recovery was blocked after the animals were subjected caffeine daily. Moreover, caffeine administration increased the demyelination area, promoted astrocyte and microglia activation and decreased the quantity of neurofilaments. These findings suggest that the neurotoxicity effect of caffeine may be associated with the inhibition of neural repair and the promotion of neuroinflammation.
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Cheng-Chang
authors.initials: CC
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan. Electronic address: jming@mail.ncku.edu.tw.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Jou
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology; D002110:Caffeine / Q000633:toxicity*; D004660:Encephalitis / Q000209:etiology / Q000503:physiopathology*; D006614:Hindlimb / Q000503:physiopathology; D007382:Intermediate Filaments / Q000187:drug effects / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000502:physiology; D009043:Motor Activity / Q000187:drug effects; D009186:Myelin Sheath / Q000187:drug effects; D058915:Purinergic P1 Receptor Antagonists / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D013119:Spinal Cord Injuries / Q000150:complications / Q000473:pathology / Q000503:physiopathology*
chemical_list: D058915:Purinergic P1 Receptor Antagonists; D002110:Caffeine
keywords: Caffeine; Spinal cord injury; Spontaneous functional recovery