title: IL-15/sIL-15Rα gene transfer induces weight loss and improves glucose homeostasis in obese mice.
abstract: Obesity and its associated metabolic problems are a major public health issue. The objective of the current study is to investigate the therapeutic effects of interleukin 15/soluble interleukin 15 receptor-α (IL-15/sIL-15Rα) on high-fat diet-induced obesity and obesity-associated metabolic disorders. We demonstrate that the multiple hydrodynamic delivery of 2 μg IL-15/sIL-15Rα plasmid results in numerous beneficial effects, including a reduction of body weight and fat mass, an alleviation of fatty liver, an improvement in glucose homeostasis and insulin sensitivity in obese mice. These effects are accompanied by a suppressed expression of genes involved in lipid accumulation and lipogenesis, including Pparγ, Cd36, Fabp4, Mgat1, Scd-1 and Fas, and elevated mRNA levels of genes involved in adaptive thermogenesis and fatty acid β-oxidation, such as Ucp1, Ucp3, Pgc-1α, Pgc-1β, Pparα, Pparδ, Cpt1-α and Cpt1-β in obese animals. These results suggest that the overexpression of the Il-15/sIl-15Rα gene is an effective approach in treating diet-induced obesity and its associated metabolic complications.
authors.affiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, USA.
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA, USA.
authors.forename: D
authors.initials: D
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D059305:Diet, High-Fat; D005234:Fatty Liver / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006706:Homeostasis; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D019409:Interleukin-15 / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D053720:Interleukin-15 Receptor alpha Subunit / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D011897:Random Allocation; D014280:Triglycerides / Q000378:metabolism; D015431:Weight Loss / Q000235:genetics*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D019409:Interleukin-15; D053720:Interleukin-15 Receptor alpha Subunit; D014280:Triglycerides