title: Mariniluteicoccus endophyticussp. nov., an endophytic actinobacterium isolated from root ofOcimum basilicum.
abstract: Two strains of Gram-stain-positive, aerobic, non-motile, irregular coccus-shaped bacteria, designated YIM 2617Tand YIM 2617-2, were isolated from the root ofOcimum basilicum. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the two strains were closely related toMariniluteicoccus flavusYIM M13146T(98.1 and 98.0 % similarities, respectively) and formed a robust clade withM. flavusin the neighbour-joining tree. Optimum growth of the strains was observed at 28-32 °C, at pH 7.0-8.0 and in the presence of 0-2 % (w/v) NaCl. The chemotaxonomic profiles of the strains comprised anteiso-C15 : 0as the major cellular fatty acid, MK-9(H4) as the predominant menaquinone, alanine, glycine, glutamic acid and ll-diaminopimelic acid as peptidoglycan components, and phosphatidylcholine and diphosphatidylglycerol as the major polar lipids. The G+C contents of the genomic DNA of strains YIM 2617Tand YIM 2617-2 were 66.4 and 66.6 mol%, respectively. DNA-DNA hybridizations of the two strains withM. flavusYIM M13146Tgave mean relatedness values of 47.6 ± 2.0 and 43.2 ± 1.5 %, respectively, while the relatedness value between them was 92.1 ± 2.2 %. On the basis of the data recorded from the present study, strains YIM 2617Tand YIM 2617-2 represent a novel species of the genusMariniluteicoccus, for which the nameMariniluteicoccus endophyticussp. nov. is proposed. The type strain is YIM 2617T( = KCTC 29482T= DSM 28728T= JCM 30097T).
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou 510275,PR China.|Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091,PR China.
authors.forename: Bing-Bing
authors.initials: BB
authors.lastname: Liu
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091,PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chu
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou 510275,PR China.
authors.forename: Nimaichand
authors.initials: N
authors.lastname: Salam
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Wei-Yao
authors.initials: WY
authors.lastname: Hu
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd,Kunming, 650231,PR China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091,PR China.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou 510275,PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li