title: Transcription of densovirus endogenous sequences in the Myzus persicae genome.
abstract: Integration of non-retroviral sequences in the genome of different organisms has been observed and, in some cases, a relationship of these integrations with immunity has been established. The genome of the green peach aphid, Myzus persicae (clone G006), was screened for densovirus-like sequence (DLS) integrations. A total of 21 DLSs localized on 10 scaffolds were retrieved that mostly shared sequence identity with two aphid-infecting viruses, Myzus persicae densovirus (MpDNV) and Dysaphis plantaginea densovirus (DplDNV). In some cases, uninterrupted potential ORFs corresponding to non-structural viral proteins or capsid proteins were found within DLSs identified in the aphid genome. In particular, one scaffold harboured a complete virus-like genome, while another scaffold contained two virus-like genomes in reverse orientation. Remarkably, transcription of some of these ORFs was observed in M. persicae, suggesting a biological effect of these viral integrations. In contrast to most of the other densoviruses identified so far that induce acute host infection, it has been reported previously that MpDNV has only a minor effect on M. persicae fitness, while DplDNV can even have a beneficial effect on its aphid host. This suggests that DLS integration in the M. persicae genome may be responsible for the latency of MpDNV infection in the aphid host.
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Clavijo
authors.affiliation: UMR 385 BGPI, INRA-CIRAD-SupAgro, CIRAD TA-A54/K, CampusInternational de Baillarguet, 34398 Montpellier, France.
authors.forename: Manuella
authors.initials: M
authors.lastname: van Munster
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Monsion
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Bochet
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Brault
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D017990:Densovirus / Q000145:classification / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics; D016662:Virus Integration
chemical_list: D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins