title: Cheese whey: A cost-effective alternative for hyaluronic acid production by Streptococcus zooepidemicus.
abstract: This study focuses on the optimisation of cheese whey formulated media for the production of hyaluronic acid (HA) by Streptococcus zooepidemicus. Culture media containing whey (W; 2.1g/L) or whey hydrolysate (WH; 2.4 g/L) gave the highest HA productions. Both W and WH produced high yields on protein consumed, suggesting cheese whey is a good nitrogen source for S. zooepidemicus production of HA. Polysaccharide concentrations of 4.0 g/L and 3.2g/L were produced in W and WH in a further scale-up to 5L bioreactors, confirming the suitability of the low-cost nitrogen source. Cheese whey culture media provided high molecular weight (>3000 kDa) HA products. This study revealed replacing the commercial peptone by the low-cost alternative could reduce HA production costs by up to a 70% compared to synthetic media.
authors.affiliation: Departamento de Química Analítica y Alimentaria, Universidad de Vigo, Ourense, Spain; CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. Electronic address: sabelara@uvigo.es.
authors.forename: Isabel R
authors.initials: IR
authors.lastname: Amado
authors.affiliation: Grupo de Reciclado y Valorización de Materiales Residuales, IIM-CSIC, Vigo, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Vázquez
authors.affiliation: International Iberian Nanotechnology Laboratory (INL), Avda. Mestre José Veiga s/n, 4715 Braga, Portugal.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Pastrana
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
mesh_terms: D002611:Cheese / Q000032:analysis*; D003470:Culture Media; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000191:economics*; D018502:Streptococcus equi / Q000378:metabolism*; D000067796:Whey / Q000737:chemistry*; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D003470:Culture Media; D000067816:Whey Proteins; D006820:Hyaluronic Acid
keywords: Agro-industrial waste; Cheese whey; Fermentation; Hyaluronic acid; Polymer; Whey protein hydrolysate