title: Metabolic biotransformation of copper-benzo[a]pyrene combined pollutant on the cellular interface of Stenotrophomonas maltophilia.
abstract: Previous studies have confirmed that Stenotrophomonas maltophilia can bind an appreciable amount of Cu(II) and degrade BaP. However, the removal mechanisms of Cu(II) coexisted with BaP by S. maltophilia are still unclear. In this study, the micro-interaction of contaminants on the cellular surface was investigated. The results indicated that carboxyl groups played an important role in the binding of copper to the thallus and that the cell walls were the main adsorption sites. Nevertheless, these reactive groups had no obvious effect on the uptake of BaP. Instead, the disruption and modification of cell walls accelerated transportation of BaP across the membrane into cells. The observation of SEM-EDS confirmed that Cu(II) would be adsorbed and precipitated onto the cell surface but would also be removed by extracellular precipitation when BaP coexisted. And the XPS analysis reflected that part of Cu(II) bound onto biosorbents changed into Cu(I) and Cu.
authors.affiliation: College of Natural Resources and Environment of South China Agricultural University, Guangzhou 510632, China; Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Shuona
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China. Electronic address: huayin@scut.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Shaoyu
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Chemistry, School of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dang
mesh_terms: D000327:Adsorption; D001564:Benzo(a)pyrene / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002462:Cell Membrane / Q000378:metabolism; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D015386:Hazardous Substances / Q000032:analysis; D020615:Stenotrophomonas maltophilia / Q000378:metabolism*; D013499:Surface Properties; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015386:Hazardous Substances; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D003300:Copper
keywords: Benzo[a]pyrene; Biological removal; Cellular interface; Combined pollutant; Copper