title: An Approach for the Selective Detection of Nitric Oxide in Biological Systems: An in vitro and in vivo Perspective.
abstract: A naphthalimide-based fluorescent probe, LyNP-NO, was designed and synthesized for the selective detection of exogenously and endogenously generated nitric oxide (NO) in C6 glial cells. In addition, LyNP-NO was also explored for monitoring endogenous NO levels in rat hippocampus at various tissue depths by stimulating the brain with N-methyl-d-aspartate (NMDA).
authors.affiliation: Department of Chemistry, Guru Nanak Dev University, Amritsar, 143005, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Department of Chemistry, Guru Nanak Dev University, Amritsar, 143005, India.
authors.forename: Shahi
authors.initials: S
authors.lastname: Imam Reja
authors.affiliation: Department of Chemistry, Guru Nanak Dev University, Amritsar, 143005, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Bhalla
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, India.
authors.forename: Muskan
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, India.
authors.forename: Gurcharan
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Chemistry, Guru Nanak Dev University, Amritsar, 143005, India. mksharmaa@yahoo.co.in.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D016202:N-Methylaspartate / Q000494:pharmacology; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D009569:Nitric Oxide; D016202:N-Methylaspartate
keywords: biological activity; fluorescent probes; imaging agents; lysosomes; nitric oxide