title: Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics.
abstract: Nogo-A and its receptors have been shown to control synaptic plasticity, including negatively regulating long-term potentiation (LTP) in the cortex and hippocampus at a fast time scale and restraining experience-dependent turnover of dendritic spines over days. However, the molecular mechanisms and the precise time course mediating these actions of Nogo-A are largely unexplored. Here we show that Nogo-A signaling in the adult nervous system rapidly modulates the spine actin cytoskeleton within minutes to control structural plasticity at dendritic spines of CA3 pyramidal neurons. Indeed, acute Nogo-A loss-of-function transiently increases F-actin stability and results in an increase in dendritic spine density and length. In addition, Nogo-A acutely restricts AMPAR insertion and mEPSC amplitude at hippocampal synaptic sites. These data indicate a crucial function of Nogo-A in modulating the very tight balance between plasticity and stability of the neuronal circuitry underlying learning processes and the ability to store long-term information in the mature CNS. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Division of Cellular Neurobiology, Zoological Institute, Braunschweig, Germany.
authors.forename: Yves
authors.initials: Y
authors.lastname: Kellner
authors.affiliation: Division of Cellular Neurobiology, Zoological Institute, Braunschweig, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Fricke
authors.affiliation: Brain Research Institute, University of Zurich, Zurich, Switzerland.|Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.
authors.forename: Stella
authors.initials: S
authors.lastname: Kramer
authors.affiliation: Division of Cellular Neurobiology, Zoological Institute, Braunschweig, Germany.
authors.forename: Cristina
authors.initials: C
authors.lastname: Iobbi
authors.affiliation: Division of Cell Biology, Faculty of Science, Utrecht University Utrecht, Netherlands.
authors.forename: Corette J
authors.initials: CJ
authors.lastname: Wierenga
authors.affiliation: Brain Research Institute, University of Zurich, Zurich, Switzerland.|Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.
authors.forename: Martin E
authors.initials: ME
authors.lastname: Schwab
authors.affiliation: Division of Cellular Neurobiology, Zoological Institute, Braunschweig, Germany.|Helmholtz Centre for Infection Research, AG NIND, Braunschweig, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Korte
authors.affiliation: Division of Cellular Neurobiology, Zoological Institute, Braunschweig, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Zagrebelsky
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D056654:CA3 Region, Hippocampal / Q000378:metabolism; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D008810:Mice, Inbred C57BL; D055369:Miniature Postsynaptic Potentials / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology; D000070798:Nogo Proteins / Q000378:metabolism*; D017966:Pyramidal Cells / Q000502:physiology; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D000199:Actins; D000070798:Nogo Proteins; D018091:Receptors, AMPA; C000603201:Rtn4 protein, mouse; C000603197:Rtn4 protein, rat
keywords: AMPA receptor insertion; FRAP; actin cytoskeleton; neurite-growth inhibitors; structural plasticity