title: One-Pot/Sequential Native Chemical Ligation Using Photocaged Crypto-thioester.
abstract: A practical and efficient methodology for the chemical synthesis of peptides/proteins using a one-pot/sequential ligation is described. It features the use of photocleavable S-protection on an N-sulfanylethylaniline moiety. Removal of the S-protecting ligated materials under UV irradiation provides a readily usable mixture for subsequent native chemical ligation.
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University , 1-78-1 Shomachi, Tokushima 770-8505, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Aihara
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University , 1-78-1 Shomachi, Tokushima 770-8505, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yamaoka
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University , 1-78-1 Shomachi, Tokushima 770-8505, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Naruse
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University , 1-78-1 Shomachi, Tokushima 770-8505, Japan.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Inokuma
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University , 1-78-1 Shomachi, Tokushima 770-8505, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shigenaga
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University , 1-78-1 Shomachi, Tokushima 770-8505, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Otaka
mesh_terms: D008026:Ligation; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D055668:Photochemical Processes; D013457:Sulfur Compounds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D010455:Peptides; D013457:Sulfur Compounds