title: Design of chimeric antigen receptors with integrated controllable transient functions.
abstract: The ability to control T cells engineered to permanently express chimeric antigen receptors (CARs) is a key feature to improve safety. Here, we describe the development of a new CAR architecture with an integrated switch-on system that permits to control the CAR T-cell function. This system offers the advantage of a transient CAR T-cell for safety while letting open the possibility of multiple cytotoxicity cycles using a small molecule drug.
authors.affiliation: Cellectis Inc, 430E, 29th street, NYC, NY 10016, USA.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Juillerat
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris.
authors.forename: Alan
authors.initials: A
authors.lastname: Marechal
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Filhol
authors.affiliation: Cellectis Inc, 430E, 29th street, NYC, NY 10016, USA.
authors.forename: Julien
authors.initials: J
authors.lastname: Valton
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris.
authors.forename: Aymeric
authors.initials: A
authors.lastname: Duclert
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirot
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris.
authors.forename: Philippe
authors.initials: P
authors.lastname: Duchateau
mesh_terms: D016827:CD8 Antigens / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000737:chemistry / Q000276:immunology; D006801:Humans; D050506:Mutant Chimeric Proteins / Q000235:genetics* / Q000276:immunology; D000072417:Protein Domains; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology; D057127:Single-Chain Antibodies / Q000235:genetics; D020123:Sirolimus / Q000031:analogs & derivatives / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D016559:Tacrolimus / Q000494:pharmacology; D014162:Transfection
chemical_list: C539629:AP 21967; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D050506:Mutant Chimeric Proteins; D011948:Receptors, Antigen, T-Cell; D057127:Single-Chain Antibodies; D020123:Sirolimus; D016559:Tacrolimus