title: High-level expression and characterization of a glycosylated human cementum protein 1 with lectin activity.
abstract: This work aims to contribute to the knowledge of human cementum protein 1 (CEMP1), its conformational characteristics and influence during the biomineralization process. The results revealed that hrCEMP1 expressed in Pichia pastoris is a 2.4% glycosylated, thermostable protein which possesses a molecular mass of 28,770 Da. The circular dichroism spectrum indicated a secondary structure content of 28.6% of alpha-helix, 9.9% of beta-sheet and 61.5% of random-coil forms. Biological activity assays demonstrated that hrCEMP1 nucleates and regulates hydroxyapatite crystal growth. Hereby, it is demonstrated for the first time that CEMP1 has a (C-type) lectin-like activity and specifically recognizes mannopyranoside. The information produced by this biochemical and structural characterization may contribute to understand more fully the biological functions of CEMP1.
authors.affiliation: Facultad de Odontología, Laboratorio de Biología Periodontal, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Romo-Arévalo
authors.affiliation: Facultad de Odontología, Laboratorio de Biología Periodontal, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Higinio
authors.initials: H
authors.lastname: Arzate
authors.affiliation: Facultad de Odontología, Laboratorio de Biología Periodontal, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Montoya-Ayala
authors.affiliation: Instituto de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Adela
authors.initials: A
authors.lastname: Rodríguez-Romero
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D004903:Erythrocyte Aggregation / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal; D006031:Glycosylation; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010843:Pichia / Q000378:metabolism*; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C510886:CEMP1 protein, human; D006882:Hydroxyapatites; D037102:Lectins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D008358:Mannose
keywords: C-type lectin-like protein; biomineralization; cementum protein 1; hydroxyapatite; periodontum; root cementum