title: Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and ethanol production.
abstract: The saccharification of ozonated sugarcane bagasse (SCB) by enzymes from Myceliophthora thermophila JCP 1-4 was studied. Fungal enzymes provided slightly higher sugar release than commercial enzymes, working at 50°C. Sugar release increased with temperature increase. Kinetic studies showed remarkable glucose release (4.99 g/L, 3%w/w dry matter) at 60°C, 8 h of hydrolysis, using an enzyme load of 10 FPU (filter paper unit). FPase and β-glucosidase activities increased during saccharification (284% and 270%, respectively). No further significant improvement on glucose release was observed increasing the enzyme load above 7.5 FPU per g of cellulose. Higher dry matter contents increased sugars release, but not yields. The fermentation of hydrolysates by Saccharomyces cerevisiae provided glucose-to-ethanol conversions around to 63%.
authors.affiliation: Department of Biology, IBILCE/UNESP - Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, São Paulo State, Brazil. Electronic address: josianipereira@gmail.com.
authors.forename: Josiani
authors.initials: J
authors.lastname: de Cassia Pereira
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid - UVa, Calle Doctor Mergelina, s/n, 47005 Valladolid, Spain. Electronic address: rtravaini@gmail.com.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Travaini
authors.affiliation: Department of Biochemistry and Chemical Technology, IQ/UNESP - Univ Estadual Paulista, Rua Prof. Francisco Degni, 55, 14800-060 Araraquara, São Paulo State, Brazil. Electronic address: nmarques@iq.unesp.br.
authors.forename: Natalia
authors.initials: N
authors.lastname: Paganini Marques
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid - UVa, Calle Doctor Mergelina, s/n, 47005 Valladolid, Spain. Electronic address: silvia@iq.uva.es.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bolado-Rodríguez
authors.affiliation: Department of Biochemistry and Chemical Technology, IQ/UNESP - Univ Estadual Paulista, Rua Prof. Francisco Degni, 55, 14800-060 Araraquara, São Paulo State, Brazil. Electronic address: dbocchini35@gmail.com.
authors.forename: Daniela Alonso
authors.initials: DA
authors.lastname: Bocchini Martins
mesh_terms: D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D031786:Saccharum / Q000378:metabolism*; D020037:Sordariales / Q000201:enzymology*; D013696:Temperature
chemical_list: D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D005947:Glucose
keywords: Enzymatic saccharification; Ethanol; Fungal enzymes; Ozonolysis; Sugarcane bagasse