title: Effects of icing or heat stress on the induction of fibrosis and/or regeneration of injured rat soleus muscle.
abstract: The effects of icing or heat stress on the regeneration of injured soleus muscle were investigated in male Wistar rats. Bupivacaine was injected into soleus muscles bilaterally to induce muscle injury. Icing (0 °C, 20 min) was carried out immediately after the injury. Heat stress (42 °C, 30 min) was applied every other day during 2-14 days after the bupivacaine injection. Injury-related increase in collagen deposition was promoted by icing. However, the level of collagen deposition in heat-stressed animals was maintained at control levels throughout the experimental period and was significantly lower than that in icing-treated animals at 15 and 28 days after bupivacaine injection. Furthermore, the recovery of muscle mass, protein content, and muscle fiber size of injured soleus toward control levels was partially facilitated by heat stress. These results suggest that, compared with icing, heat stress may be a beneficial treatment for successful muscle regeneration at least by reducing fibrosis.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-17 Machikaneyama-cho, Toyonaka City, Osaka, 560-0043, Japan. wing4897@gmail.com.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Shibaguchi
authors.affiliation: Department of Exercise and Health Sciences, Faculty of Education, Yamaguchi University, Yamaguchi City, Yamaguchi, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Department of Exercise and Health Sciences, Faculty of Education, Yamaguchi University, Yamaguchi City, Yamaguchi, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Fujitsu
authors.affiliation: Department of Exercise and Health Sciences, Faculty of Education, Yamaguchi University, Yamaguchi City, Yamaguchi, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Nomura
authors.affiliation: Graduate School of Health and Sports Science, Juntendo University, Inzai City, Chiba, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Yoshihara
authors.affiliation: Graduate School of Health and Sports Science, Juntendo University, Inzai City, Chiba, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Naito
authors.affiliation: Hirosaki Gakuin University, Hirosaki City, Aomori, Japan.
authors.forename: Toshitada
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-17 Machikaneyama-cho, Toyonaka City, Osaka, 560-0043, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ogura
authors.affiliation: Graduate School of Health and Sports Science, Doshisha University, Kyotanabe City, Kyoto, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ohira
mesh_terms: D000818:Animals; D003080:Cold Temperature; D017679:Cryotherapy; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature / Q000627:therapeutic use*; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*
chemical_list: D050884:HSP72 Heat-Shock Proteins
keywords: Fibrosis; Heat shock protein 72; Heat stress; Icing; Muscle regeneration; Satellite cell