title: Heterologous complementation studies reveal the solute transport profiles of a two-member nucleobase cation symporter 1 (NCS1) family in Physcomitrella patens.
abstract: As part of an evolution-function analysis, two nucleobase cation symporter 1 (NCS1) from the moss Physcomitrella patens (PpNCS1A and PpNCS1B) are examined--the first such analysis of nucleobase transporters from early land plants. The solute specificity profiles for the moss NCS1 were determined through heterologous expression, growth and radiolabeled uptake experiments in NCS1-deficient Saccharomyces cerevisiae. Both PpNCS1A and 1B, share the same profiles as high affinity transporters of adenine and transport uracil, guanine, 8-azaguanine, 8-azaadenine, cytosine, 5-fluorocytosine, hypoxanthine, and xanthine. Despite sharing the same solute specificity profile, PpNCS1A and PpNCS1B move nucleobase compounds with different efficiencies. The broad nucleobase transport profile of PpNCS1A and 1B differs from the recently-characterized Viridiplantae NCS1 in breadth, revealing a flexibility in solute interactions with NCS1 across plant evolution.
authors.affiliation: Department of Biology, Indiana University-Purdue University Fort Wayne, 2101 East Coliseum Blvd., Fort Wayne, IN 46805, USA.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Minton
authors.affiliation: Department of Biology, Indiana University-Purdue University Fort Wayne, 2101 East Coliseum Blvd., Fort Wayne, IN 46805, USA.
authors.forename: Micah
authors.initials: M
authors.lastname: Rapp
authors.affiliation: Department of Biology, Indiana University-Purdue University Fort Wayne, 2101 East Coliseum Blvd., Fort Wayne, IN 46805, USA.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Stoffer
authors.affiliation: Department of Plant Pathology and Ecology, The Connecticut Agricultural Experiment Station, 123 Huntington St, New Haven, CT 06511, USA.
authors.forename: Neil P
authors.initials: NP
authors.lastname: Schultes
authors.affiliation: Department of Biology, Indiana University-Purdue University Fort Wayne, 2101 East Coliseum Blvd., Fort Wayne, IN 46805, USA. Electronic address: mourad@ipfw.edu.
authors.forename: George S
authors.initials: GS
authors.lastname: Mourad
mesh_terms: D019068:Bryopsida / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D033702:Nucleobase Transport Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D033702:Nucleobase Transport Proteins; D010940:Plant Proteins
keywords: Nucleobase cation symporter 1; Physcomitrella patens; Purine and pyrimidine; Transport