title: Prevalence of Borrelia miyamotoi in Ixodes persulcatus in Irkutsk City and its neighboring territories, Russia.
abstract: Adult Ixodes persulcatus were collected in highly populated districts in Irkutsk city, Russia, and in popular recreational and professional areas in its neighboring territories. Borrelia miyamotoi infection in I. persulcatus was examined using multiplex Taqman-PCR targeting 16S rDNA, and nested PCR and sequencing analyses targeting flaB and 16S rDNA. B. miyamotoi and Lyme disease Borrelia species were detected in 13 (infection rate, 2.9%) and 77 (17.3%) out of 445 I. persulcatus ticks, respectively, collected from 4 sites around the Baikal Lake. The 16S rDNA and flaB sequences of these amplicons were closely related to those of B. miyamotoi detected and/or isolated from I. persulcatus in Japan and Far Eastern Russia, and clustered separately from those of Europe and North America. These results indicate that additional surveillance for B. miyamotoi infection is needed in order to determine how it affects human health in Irkutsk City and its neighboring territories.
authors.affiliation: Federal State Public Science Institution, Scientific Centre of Family Health and Human Reproduction Problems, Irkutsk, Russia.
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Khasnatinov
authors.affiliation: Federal State Public Science Institution, Scientific Centre of Family Health and Human Reproduction Problems, Irkutsk, Russia.
authors.forename: Galina A
authors.initials: GA
authors.lastname: Danchinova
authors.affiliation: Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Takano
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan; United Graduate School of Agricultural Science & Veterinary Science, Gifu University, Gifu, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kawabata
authors.affiliation: School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Ohashi
authors.affiliation: Faculty of Pharmaceutical Sciences, Chiba Institute of Science (CIS), Choshi, Japan. Electronic address: masuzawat@cis.ac.jp.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Masuzawa
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001898:Borrelia / Q000235:genetics / Q000302:isolation & purification*; D005408:Flagellin / Q000235:genetics; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S; C064192:flaB1 protein, bacteria; D005408:Flagellin
keywords: Borrelia miyamotoi; Irkutsk; Ixodes persulcatus; Tick