title: One-pot peptide and protein conjugation: a combination of enzymatic transamidation and click chemistry.
abstract: Enzymatic transamidation and copper-catalyzed azide-alkyne cycloaddition (CuAAC) were combined to yield covalently conjugated peptides and proteins. The addition of glutathione preserved enzymatic activity in the presence of copper. Tuning the reaction kinetics was key to success, providing up to 95% conversion. This one-pot reaction allowed for targeted fluorescent protein labeling.
authors.affiliation: Department of Chemistry, Université de Montréal, 2900 Boulevard Edouard-Montpetit, Montréal, Québec H3T 1J4, Canada. joelle.pelletier@umontreal.ca.
authors.forename: N M
authors.initials: NM
authors.lastname: Rachel
authors.forename: J N
authors.initials: JN
authors.lastname: Pelletier
mesh_terms: D057930:Click Chemistry; D004798:Enzymes / Q000378:metabolism*; D005978:Glutathione / Q000737:chemistry; D007700:Kinetics; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D004798:Enzymes; D010455:Peptides; D011506:Proteins; D005978:Glutathione