title: Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2).
abstract: OBJECTIVE
  Although tonsil-mesenchymal stem cells (T-MSCs) have been studied as a new autologous or homologous source of MSCs, research on specific markers of MSCs and localization for purified T-MSC isolation has not yet been reported. This study investigates the expression of W5C5 (SUSD2) in tonsil stromal cells and the colony-forming ability and differentiation potential of W5C5+ cells to determine the usefulness of W5C5+ MSCs as a marker that can be used for the purification of T-MSCs. In addition, the location of W5C5+ cells expressed in the tonsil tissues is examined.
  
  
  METHODS
  T-MSCs were isolated from the tonsillar tissues of 12 patients undergoing tonsillectomy. The colony-forming ability, surface markers, proliferation potential, and differentiation capacities of purified W5C5+ MSCs, W5C5- MSCs, and unselected T-MSCs were evaluated. The location of the W5C5+ cells in the tonsillar tissues was also investigated by immunohistochemistry.
  
  
  RESULTS
  W5C5 was expressed in 2.5±0.4% of fresh human tonsil stromal cells. W5C5+ cells formed many colonies, but W5C5- cells did not form any colonies. The colony-forming number of W5C5+ cells (74.4 ± 9.8) was significantly higher than that of unselected tonsil stromal cells (23.6 ± 3.7). However, the differences in proliferation potential, surface marker expression, and differentiation potential between W5C5+ T-MSCs and unselected T-MSCs were not significant. W5C5+ cells were identified in the perivascular area around the blood vessels.
  
  
  CONCLUSIONS
  W5C5+ T-MSCs possessed typical MSC properties with high colony-forming efficiency, and niches of W5C5+ T-MSCs were located in the perivascular area of tonsil tissues. These findings suggest that W5C5 is a useful single marker for the isolation of purified T-MSCs.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University, Busan, Korea.
authors.forename: Byung-Joo
authors.initials: BJ
authors.lastname: Lee
authors.forename: Dae-Woon
authors.initials: DW
authors.lastname: Kang
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Park
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Song
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Jeon-Yeob
authors.initials: JY
authors.lastname: Jang
authors.forename: Jin-Choon
authors.initials: JC
authors.lastname: Lee
authors.forename: Soo-Geun
authors.initials: SG
authors.lastname: Wang
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
authors.forename: Sung-Chan
authors.initials: SC
authors.lastname: Shin
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D014066:Palatine Tonsil / Q000166:cytology*
chemical_list: D015703:Antigens, CD; D008562:Membrane Glycoproteins; C582626:SUSD2 protein, human