title: Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG.
abstract: This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Baek
authors.affiliation: National Institute of Animal Resource, RDA, Suwon 441-706, Korea.
authors.forename: Sung Kwon
authors.initials: SK
authors.lastname: Park
authors.affiliation: D&P Biotech, Daegu 700-422, Korea.
authors.forename: Myung Hoon
authors.initials: MH
authors.lastname: Lee
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
authors.forename: Sung Il
authors.initials: SI
authors.lastname: Kim
authors.affiliation: National Institute of Animal Resource, RDA, Suwon 441-706, Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Cho
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
authors.forename: Chang Bon
authors.initials: CB
authors.lastname: Choi
keywords: BIO-TAG; Hanwoo; rapid diagnosis kit; synthetic peptide