title: RNA Helicase Associated with AU-rich Element (RHAU/DHX36) Interacts with the 3'-Tail of the Long Non-coding RNA BC200 (BCYRN1).
abstract: RNA helicase associated with AU-rich element (RHAU) is an ATP-dependent RNA helicase that demonstrates high affinity for quadruplex structures in DNA and RNA. To elucidate the significance of these quadruplex-RHAU interactions, we have performed RNA co-immunoprecipitation screens to identify novel RNAs bound to RHAU and characterize their function. In the course of this study, we have identified the non-coding RNA BC200 (BCYRN1) as specifically enriched upon RHAU immunoprecipitation. Although BC200 does not adopt a quadruplex structure and does not bind the quadruplex-interacting motif of RHAU, it has direct affinity for RHAU in vitro. Specifically designed BC200 truncations and RNase footprinting assays demonstrate that RHAU binds to an adenosine-rich region near the 3'-end of the RNA. RHAU truncations support binding that is dependent upon a region within the C terminus and is specific to RHAU isoform 1. Tests performed to assess whether BC200 interferes with RHAU helicase activity have demonstrated the ability of BC200 to act as an acceptor of unwound quadruplexes via a cytosine-rich region near the 3'-end of the RNA. Furthermore, an interaction between BC200 and the quadruplex-containing telomerase RNA was confirmed by pull-down assays of the endogenous RNAs. This leads to the possibility that RHAU may direct BC200 to bind and exert regulatory functions at quadruplex-containing RNA or DNA sequences.
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Evan P
authors.initials: EP
authors.lastname: Booy
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Ewan K S
authors.initials: EK
authors.lastname: McRae
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Ryan
authors.initials: R
authors.lastname: Howard
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Soumya R
authors.initials: SR
authors.lastname: Deo
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Emmanuel O
authors.initials: EO
authors.lastname: Ariyo
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Edis
authors.initials: E
authors.lastname: Dzananovic
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Markus
authors.initials: M
authors.lastname: Meier
authors.affiliation: From the Departments of Chemistry and Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
authors.forename: Jörg
authors.initials: J
authors.lastname: Stetefeld
authors.affiliation: From the Departments of Chemistry and Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada sean.mckenna@umanitoba.ca.
authors.forename: Sean A
authors.initials: SA
authors.lastname: McKenna
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D054856:G-Quadruplexes; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008969:Molecular Sequence Data; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D062085:RNA, Long Noncoding; C000609640:long non-coding RNA BC200, human; C466988:DHX36 protein, human; D053487:DEAD-box RNA Helicases
keywords: BC200; BCYRN1; DHX36; G-quadruplex; RHAU; RNA helicase; RNA structure; RNA-protein interaction; long noncoding RNA (long ncRNA, lncRNA); telomerase RNA