title: In vitro dioxin-like potencies of HO- and MeO-PBDEs and inter-species sensitivity variation in birds.
abstract: Due to their bioaccumulative properties, hydroxylated and methoxylated polybrominated diphenyl ethers (HO-/MeO-PBDEs) may pose ecological risks to wild life, including birds. However, their toxicity potencies in avian species are largely unknown. In the present study, an avian AHR1 luciferase reporter gene (LRG) assay with luciferase probes from chicken, pheasant and quail was used to test activations of avian aryl hydrocarbon receptor (AHR)-mediated pathways by 19 HO- or MeO-PBDEs in different avian species. Species-specific relative potencies (RePs) of HO-/MeO-PBDEs to tetrachlorodibenzo-p-dioxin (TCDD) and relative sensitivities of various species to each chemical were estimated. The results indicated that the ReP of the most potent HO-/MeO-PBDEs, 5-Cl-6-HO-BDE-47, was 7.8×10(-4) for chicken, 1.1×10(-2) for pheasant, and 1.7×10(-1) for quail comparing to TCDD. In addition, it was found that avian species with the greatest sensitivity to TCDD did not always have the greatest sensitivity to HO-/MeO-PBDEs and vice versa. This study contributed to filling in the knowledge gap regarding the dioxin-like activity of HO-/MeO-PBDEs in birds, and provided beneficial information for the prioritization of HO-/MeO-PBDEs for further research.
  HO-/MeO-PBDEs activate avian AHR-mediated pathways in a congener- and species- specific manner. 5-Cl-6-HO-BDE-47 was the most potent among the nineteen HO-/MeO-PBDEs tested.
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
authors.forename: Junjiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China. Electronic address: howard50003250@yahoo.com.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
authors.forename: Guanyong
authors.initials: G
authors.lastname: Su
authors.affiliation: Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5.
authors.forename: Reza
authors.initials: R
authors.lastname: Farmahin
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China; Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada S7N 5B3.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D019556:COS Cells; D002645:Chickens / Q000378:metabolism; D002522:Chlorocebus aethiops; D004147:Dioxins / Q000633:toxicity; D046094:Galliformes / Q000378:metabolism; D017930:Genes, Reporter; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry / Q000633:toxicity*; D006900:Hydroxylation; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D011784:Quail / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D013045:Species Specificity
chemical_list: D004147:Dioxins; D055768:Halogenated Diphenyl Ethers; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon
keywords: Emerging chemicals; High-throughput screening; Prioritization; Relative potency; Species sensitivity