title: Singlet Oxygen-Induced Cell Death in Arabidopsis under High-Light Stress Is Controlled by OXI1 Kinase.
abstract: Studies of the singlet oxygen ((1)O2)-overproducing flu and chlorina1 (ch1) mutants of Arabidopsis (Arabidopsis thaliana) have shown that (1)O2-induced changes in gene expression can lead to either programmed cell death (PCD) or acclimation. A transcriptomic analysis of the ch1 mutant has allowed the identification of genes whose expression is specifically affected by each phenomenon. One such gene is OXIDATIVE SIGNAL INDUCIBLE1 (OXI1) encoding an AGC kinase that was noticeably induced by excess light energy and (1)O2 stress conditions leading to cell death. Photo-induced oxidative damage and cell death were drastically reduced in the OXI1 null mutant (oxi1) and in the double mutant ch1*oxi1 compared with the wild type and the ch1 single mutant, respectively. This occurred without any changes in the production rate of (1)O2 but was cancelled by exogenous applications of the phytohormone jasmonate. OXI1-mediated (1)O2 signaling appeared to operate through a different pathway from the previously characterized OXI1-dependent response to pathogens and H2O2 and was found to be independent of the EXECUTER proteins. In high-light-stressed plants, the oxi1 mutation was associated with reduced jasmonate levels and with the up-regulation of genes encoding negative regulators of jasmonate signaling and PCD. Our results show that OXI1 is a new regulator of (1)O2-induced PCD, likely acting upstream of jasmonate.
authors.affiliation: CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementales, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);Aix-Marseille Université, F-13284 Marseille, France (L.S., A.C., B.L., F.M., M.H.);POPS Transcriptomic Platform, Institute of Plant Sciences Paris-Saclay IPS2, Rue de Noetzlin, 91405 Orsay, France (L.T.); andUniversité Avignon et des Pays de Vaucluse, 84000 Avignon, France (F.M.).
authors.forename: Leonard
authors.initials: L
authors.lastname: Shumbe
authors.affiliation: CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementales, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);Aix-Marseille Université, F-13284 Marseille, France (L.S., A.C., B.L., F.M., M.H.);POPS Transcriptomic Platform, Institute of Plant Sciences Paris-Saclay IPS2, Rue de Noetzlin, 91405 Orsay, France (L.T.); andUniversité Avignon et des Pays de Vaucluse, 84000 Avignon, France (F.M.).
authors.forename: Anne
authors.initials: A
authors.lastname: Chevalier
authors.affiliation: CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementales, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);Aix-Marseille Université, F-13284 Marseille, France (L.S., A.C., B.L., F.M., M.H.);POPS Transcriptomic Platform, Institute of Plant Sciences Paris-Saclay IPS2, Rue de Noetzlin, 91405 Orsay, France (L.T.); andUniversité Avignon et des Pays de Vaucluse, 84000 Avignon, France (F.M.).
authors.forename: Bertrand
authors.initials: B
authors.lastname: Legeret
authors.affiliation: CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementales, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);Aix-Marseille Université, F-13284 Marseille, France (L.S., A.C., B.L., F.M., M.H.);POPS Transcriptomic Platform, Institute of Plant Sciences Paris-Saclay IPS2, Rue de Noetzlin, 91405 Orsay, France (L.T.); andUniversité Avignon et des Pays de Vaucluse, 84000 Avignon, France (F.M.).
authors.forename: Ludivine
authors.identifier: http://orcid.org/0000-0001-7912-7220
authors.initials: L
authors.lastname: Taconnat
authors.affiliation: CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementales, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);Aix-Marseille Université, F-13284 Marseille, France (L.S., A.C., B.L., F.M., M.H.);POPS Transcriptomic Platform, Institute of Plant Sciences Paris-Saclay IPS2, Rue de Noetzlin, 91405 Orsay, France (L.T.); andUniversité Avignon et des Pays de Vaucluse, 84000 Avignon, France (F.M.).
authors.forename: Fabien
authors.initials: F
authors.lastname: Monnet
authors.affiliation: CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementales, F-13108 Saint-Paul-lez-Durance, France (L.S., A.C., B.L., F.M., M.H.);Aix-Marseille Université, F-13284 Marseille, France (L.S., A.C., B.L., F.M., M.H.);POPS Transcriptomic Platform, Institute of Plant Sciences Paris-Saclay IPS2, Rue de Noetzlin, 91405 Orsay, France (L.T.); andUniversité Avignon et des Pays de Vaucluse, 84000 Avignon, France (F.M.) michel.havaux@cea.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Havaux
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008027:Light; D008957:Models, Genetic; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000494:pharmacology; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D026082:Singlet Oxygen / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D045332:Photosystem II Protein Complex; D010937:Plant Growth Regulators; D026082:Singlet Oxygen; C011006:jasmonic acid; C482589:OXI1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases