title: Lysobacter terricolasp. nov., isolated from greenhouse soil.
abstract: Strain 5GH22-11T, which was isolated from greenhouse soil in the Yangpyeong region, Gyeonggi province, Republic of Korea, was characterized to be an aerobic, Gram-stain-negative, flagellated, rod-shaped bacterium. It could grow at temperatures from 10 to 33 °C (optimum of 28-30 °C), in the pH range of 6.0-10.0 (optimum of pH 7.0) and without NaCl. 16S rRNA gene sequence analysis showed that strain 5GH18-14Tshowed the highest sequence similarities withLysobacter niabensisGH34-4T(98.6 %),Lysobacter yangpyeongensisGH19-3T(98.1 %), 'Lysobacter fragariae' THG-DN8.7 (97.9 %),Lysobacter terraeTHG-A13T(97.3 %), 'Lysobacter rhizosphaerae' THG-DN8.3 (97.2 %), 'Lysobacter tyrosinelyticus' THG-DN8.2 (97.2 %) andLysobacter oryzaeYC6269T(97.2 %), revealing less than 95.5 % sequence similarities with all other species with validly published names. Phylogenetic trees also indicated that strain 5GH18-14Tformed a compact subcluster withL. niabensisGH34-4T,L. yangpyeongensisGH19-3T,L. terraeTHG-A13TandL. oryzaeYC6269Twithin the genusLysobacter. The predominant quinone of strain 5GH18-14Twas Q-8. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmonomethylethanolamine in large amounts, and moderate or small amounts of three unknown phospholipids and two unknown aminophospholipids. DNA-DNA hybridization values with closely related species were below 70 %. The DNA G+C content was 65.9 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it has been demonstrated that strain 5GH18-14Trepresents a novel species of the genusLysobacter, for which the nameLysobacter terricolasp. nov. is proposed. The type strain is 5GH18-14T( = KACC 16954T= JCM 30862T).
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Seung-Beom
authors.initials: SB
authors.lastname: Hong
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Soon-Ja
authors.initials: SJ
authors.lastname: Seok
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Jeong-Seon
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun,Jeollabuk-do 55365,Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon