title: Effects of Dietary Copper-Methionine on Matrix Metalloproteinase-2 in the Lungs of Cold-Stressed Broilers as an Animal Model for Pulmonary Hypertension.
abstract: The objective of the present study was to investigate the effects of different levels of copper (as supplemental copper-methionine) on ascites incidence and matrix metalloproteinase-2 (MMP-2) changes in the lungs of cold-stressed broilers. For this purpose, 480 1-day-old Ross 308 broiler chickens were randomly assigned to six treatments. Treatments consisted of two ambient temperatures (thermoneutral and cold stress) each combined with 0, 100, and 200 mg supplemental copper/kg as copper-methionine in a 2 × 3 factorial arrangement in a completely randomized design with four replicates. Ascites was diagnosed based on abdominal and pericardial fluid accumulation at 45 days of age. Fourty-eight broilers were killed at 38 and 45 days of age, and their lungs were collected for biological analysis. Results showed that MMP-2 increased in the lungs of ascitic broilers and that copper-methionine supplementation significantly reduced MMP-2 in cold-stressed broiler chickens. Treatments did not affect tissue inhibitor of metalloproteinase-2 (TIMP-2) at 38 and 45 days of age, and no difference was observed between 100 and 200 mg/kg copper-methionine treatments. In conclusion, copper-methionine at higher than conventional levels of supplementation decreased ascites incidence in low temperature through reduced MMP-2 concentration. Further research is warranted to investigate the effect of copper on MMP-2 concentrations in other tissues with high oxygen demand.
authors.affiliation: Department of Animal Sciences, Isfahan University of Technology, Isfahan, 84156-83111, Iran. mina_b641028@yahoo.com.|Unité de Recherche "Matrice Extracellulaire et Dynamique Cellulaire" (MEDyC), UMR CNRS/URCA NO 7369, Faculté de Médecine de Reims, 51095, Reims Cedex, France. mina_b641028@yahoo.com.
authors.forename: Mina
authors.initials: M
authors.lastname: Bagheri Varzaneh
authors.affiliation: Department of Animal Sciences, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Rahmani
authors.affiliation: Department of Animal Sciences, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Rahman
authors.initials: R
authors.lastname: Jahanian
authors.affiliation: Department of Animal Sciences, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Amir Hossein
authors.initials: AH
authors.lastname: Mahdavi
authors.affiliation: Unité de Recherche "Matrice Extracellulaire et Dynamique Cellulaire" (MEDyC), UMR CNRS/URCA NO 7369, Faculté de Médecine de Reims, 51095, Reims Cedex, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Perreau
authors.affiliation: Unité de Recherche "Matrice Extracellulaire et Dynamique Cellulaire" (MEDyC), UMR CNRS/URCA NO 7369, Faculté de Médecine de Reims, 51095, Reims Cedex, France.
authors.forename: Gwenn
authors.initials: G
authors.lastname: Perrot
authors.affiliation: Unité de Recherche "Matrice Extracellulaire et Dynamique Cellulaire" (MEDyC), UMR CNRS/URCA NO 7369, Faculté de Médecine de Reims, 51095, Reims Cedex, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Brézillon
authors.affiliation: Unité de Recherche "Matrice Extracellulaire et Dynamique Cellulaire" (MEDyC), UMR CNRS/URCA NO 7369, Faculté de Médecine de Reims, 51095, Reims Cedex, France.|CHU de Reims, Laboratoire Central de Biochimie, 51092, Reims Cedex, France.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Maquart
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003300:Copper / Q000008:administration & dosage / Q000494:pharmacology*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006976:Hypertension, Pulmonary / Q000188:drug therapy* / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D008715:Methionine / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D003300:Copper; D008715:Methionine; D020778:Matrix Metalloproteinase 2
keywords: Ascites; Copper-methionine; Matrix metalloproteinase-2 (MMP-2); Tissue inhibitor of metalloproteinase-2 (TIMP-2)