title: Lactobacillus acidophilus modulates inflammatory activity by regulating the TLR4 and NF-κB expression in porcine peripheral blood mononuclear cells after lipopolysaccharide challenge.
abstract: A total of forty weaned pigs ((Landrace × Yorkshire) × Duroc) were used to evaluate the effects of Lactobacillus acidophilus on inflammatory activity after lipopolysaccharide (LPS) challenge. Experimental treatments were as follows: (T1) control diet+saline challenge; (T2) control diet with 0·1% L. acidophilus+saline challenge; (T3) control diet+LPS challenge; and (T4) control diet with 0·1% L. acidophilus+LPS challenge. On d-14, piglets were challenged with saline (T1 and T2) or LPS (T3 and T4). Blood samples were obtained at 0, 2, 4, 6 and 12 h after being challenged and analysed for immune cell cytokine production and gene expression pattern. The L. acidophilus treatment increased the average daily weight gain (ADWG) and average daily feed intake (ADFI) compared with the control diet. With the control diet, the LPS challenge (T3) increased the number of immune cells and expression of TNF-α and IL-6 compared with the saline challenge (T1). Whereas with the saline challenge L. acidophilus treatment (T2) increased the number of leucocytes and CD4 compared with the control diet (T1), with the LPS challenge L. acidophilus treatment (T4) decreased the number of leucocytes, lymphocytes, CD4+ and CD8+ and expression of TNF-α and IL-6 compared with the control diet (T3). L. acidophilus treatment decreased the expression of TRL4 and NF-κB in peripheral blood mononuclear cells (PBMC) after LPS challenge, which leads to inhibition of TNF-α, IFN-γ, IL-6, IL-8 and IL1B1 and to induction of IL-4 and IL-10. We suggested that L. acidophilus improved ADWG and ADFI and protected against LPS-induced inflammatory responses by regulating TLR4 and NF-κB expression in porcine PBMC.
authors.affiliation: Department of Animal Resource and Science,Dankook University,Cheonan,Choongnam,330-714,Republic of Korea.
authors.forename: Sang In
authors.initials: SI
authors.lastname: Lee
authors.affiliation: Department of Animal Resource and Science,Dankook University,Cheonan,Choongnam,330-714,Republic of Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Animal Resource and Science,Dankook University,Cheonan,Choongnam,330-714,Republic of Korea.
authors.forename: Jin Mo
authors.initials: JM
authors.lastname: Koo
authors.affiliation: Department of Animal Resource and Science,Dankook University,Cheonan,Choongnam,330-714,Republic of Korea.
authors.forename: In Ho
authors.initials: IH
authors.lastname: Kim
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects; D000821:Animal Feed / Q000009:adverse effects / Q000382:microbiology*; D000818:Animals; D003433:Crosses, Genetic; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D006130:Growth Disorders / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control / Q000662:veterinary; D007113:Immunity, Innate / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007779:Lactobacillus acidophilus / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D016328:NF-kappa B / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D056910:Republic of Korea; D034421:Sus scrofa; D051197:Toll-Like Receptor 4 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D014886:Weaning; D015430:Weight Gain / Q000187:drug effects
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051197:Toll-Like Receptor 4; C482199:lipopolysaccharide, E coli O55-B5
keywords: ADFI average daily feed intake; ADWG average daily weight gain; Inflammatory cytokines; LPS lipopolysaccharide; Lactobacillus acidophilus; Lipopolysaccharide; PBMC peripheral blood mononuclear cell; Peripheral blood mononuclear cells; TLR toll-like receptors; Toll-like receptor 4; qRT-PCR quantitative real-time PCR