title: Aberrant Wound Healing in an Epidermal Interleukin-4 Transgenic Mouse Model of Atopic Dermatitis.
abstract: Wound healing in a pre-existing Th2-dominated skin milieu was assessed by using an epidermal specific interleukin-4 (IL-4) transgenic (Tg) mouse model, which develops a pruritic inflammatory skin condition resembling human atopic dermatitis. Our results demonstrated that IL-4 Tg mice had delayed wound closure and re-epithelialization even though these mice exhibited higher degrees of epithelial cell proliferation. Wounds in IL-4 Tg mice also showed a marked enhancement in expression of inflammatory cytokines/chemokines, elevated infiltration of inflammatory cells including neutrophils, macrophages, CD3+ lymphocytes, and epidermal dendritic T lymphocytes. In addition, these mice exhibited a significantly higher level of angiogenesis as compared to wild type mice. Furthermore, wounds in IL-4 Tg mice presented with larger amounts of granulation tissue, but had less expression and deposition of collagen. Taken together, an inflamed skin condition induced by IL-4 has a pronounced negative influence on the healing process. Understanding more about the pathogenesis of wound healing in a Th2- dominated environment may help investigators explore new potential therapeutic strategies.
authors.affiliation: Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, Illinois, United States of America.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Departments of Dermatology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States of America.
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.affiliation: Departments of Dermatology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States of America.|Departments of Immunology and Microbiology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States of America.|Medicine Service, Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois, United States of America.
authors.forename: Lawrence S
authors.initials: LS
authors.lastname: Chan
authors.affiliation: Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, Illinois, United States of America.
authors.forename: Luisa A
authors.initials: LA
authors.lastname: DiPietro
authors.affiliation: Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, Illinois, United States of America.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000235:genetics / Q000503:physiopathology*; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D006097:Granulation Tissue / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009504:Neutrophils / Q000378:metabolism; D062326:Re-Epithelialization / Q000235:genetics / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics / Q000502:physiology
chemical_list: D016207:Cytokines; D015847:Interleukin-4