title: SOX11 and HIG-2 are cross-regulated and affect growth in mantle cell lymphoma.
abstract: The transcriptional factor SOX11 is a disease-defining antigen in mantle cell lymphoma (MCL) and absent in most non-malignant tissues. To explore the role of SOX11-related cell signaling, and potentially take benefit from these for targeted therapy, associated networks and proteins need to be defined. In this study, we used an inducible SOX11 knock-down system followed by gene expression analysis to identify co-regulated genes and associated signaling pathways. A limited number (n = 27) of significantly co-regulated genes were identified, including SETMAR, HIG-2, and CD24. Further analysis confirmed co-regulation of SOX11 with HIG-2 and CD24 at the protein level. Of major interest, knock-down of HIG-2 reduced SOX11 levels and increased proliferation, the proteins are thus cross-regulated. HIG-2 was localized at the plasma cell membrane in both cell lines and primary MCL cells, and could potentially be of interest for targeted therapy.
authors.affiliation: a Department of Immunotechnology , Lund University , Lund , Sweden ;|b CREATE Health, Lund University , Lund , Sweden.
authors.forename: Venera
authors.initials: V
authors.lastname: Kuci
authors.affiliation: a Department of Immunotechnology , Lund University , Lund , Sweden ;|b CREATE Health, Lund University , Lund , Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Nordström
authors.affiliation: a Department of Immunotechnology , Lund University , Lund , Sweden ;|b CREATE Health, Lund University , Lund , Sweden.
authors.forename: Paolo
authors.initials: P
authors.lastname: Conrotto
authors.affiliation: a Department of Immunotechnology , Lund University , Lund , Sweden ;|b CREATE Health, Lund University , Lund , Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Ek
mesh_terms: D051927:CD24 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism; D061251:Primary Cell Culture; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014161:Transduction, Genetic
chemical_list: D051927:CD24 Antigen; C497889:CD24 protein, human; C405620:HILPDA protein, human; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C097473:SOX11 protein, human; D055751:SOXC Transcription Factors
keywords: HIG-2; SETMAR and CD24; SOX11