title: Investigation on the Interaction of Hydroxyethyl Starch 130/0.4 (Voluven) and Serum Albumin for Pharmacokinetic and Toxicological Implications.
abstract: The interaction of hydroxyethyl starch 130/0.4 (Voluven) with human serum albumin (HSA) has been investigated by fluorescence (steady state and synchronous), Fourier transforms infrared (FT-IR), and circular dichroism (CD) spectroscopies. Analysis of the fluorescence quenching data of HSA by Voluven using the Stern-Volmer method revealed the formation of 1:1 ground-state complex. Evaluation of binding parameters and binding energy indicated that the binding reaction was exothermic. On the basis of fluorescence measurements, it was concluded that electrostatic forces play a crucial role in stabilizing the complex. The binding distance was calculated by using Förster resonance energy transfer (FRET) theory. The conformational changes of HSA were obtained qualitatively as well as quantitatively using synchronous fluorescence, FT-IR, and CD. The HSA underwent partial unfolding in the presence of Voluven.
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. profzhijianfu@163.com.|Yantaishan Hospital, Yantai, 264000, People's Republic of China. profzhijianfu@163.com.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Yantaishan Hospital, Yantai, 264000, People's Republic of China.
authors.forename: Shaoyan
authors.initials: S
authors.lastname: Huang
authors.affiliation: People's Hospital of Rizhao, Rizhao, 276826, People's Republic of China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Han
authors.affiliation: Yantaishan Hospital, Yantai, 264000, People's Republic of China.
authors.forename: Chenghai
authors.initials: C
authors.lastname: Wang
authors.affiliation: Yantaishan Hospital, Yantai, 264000, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Fu
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000737:chemistry*; D008958:Models, Molecular; D011485:Protein Binding; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: C485123:HES 130-0.4; D006895:Hydroxyethyl Starch Derivatives; D012709:Serum Albumin
keywords: Circular dichroism; FT-IR; Fluorescence; Human serum albumin; Hydroxyethyl starch