title: 7-Rhamnosylated Flavonols Modulate Homeostasis of the Plant Hormone Auxin and Affect Plant Development.
abstract: Flavonols are a group of secondary metabolites that affect diverse cellular processes. They are considered putative negative regulators of the transport of the phytohormone auxin, by which they influence auxin distribution and concomitantly take part in the control of plant organ development. Flavonols are accumulating in a large number of glycosidic forms. Whether these have distinct functions and diverse cellular targets is not well understood. The rol1-2 mutant of Arabidopsis thaliana is characterized by a modified flavonol glycosylation profile that is inducing changes in auxin transport and growth defects in shoot tissues. To determine whether specific flavonol glycosides are responsible for these phenotypes, a suppressor screen was performed on the rol1-2 mutant, resulting in the identification of an allelic series of UGT89C1, a gene encoding a flavonol 7-O-rhamnosyltransferase. A detailed analysis revealed that interfering with flavonol rhamnosylation increases the concentration of auxin precursors and auxin metabolites, whereas auxin transport is not affected. This finding provides an additional level of complexity to the possible ways by which flavonols influence auxin distribution and suggests that flavonol glycosides play an important role in regulating plant development.
authors.affiliation: From the Department of Plant and Microbial Biology, University of Zurich, 8008 Zurich, Switzerland.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Kuhn
authors.affiliation: From the Department of Plant and Microbial Biology, University of Zurich, 8008 Zurich, Switzerland.
authors.forename: Sanae
authors.initials: S
authors.lastname: Errafi
authors.affiliation: the Department of Chemistry, University of Zurich, 8057 Zurich, Switzerland.
authors.forename: Rahel
authors.initials: R
authors.lastname: Bucher
authors.affiliation: the Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic, and.
authors.forename: Petre
authors.initials: P
authors.lastname: Dobrev
authors.affiliation: the Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Geisler
authors.affiliation: the Department of Chemistry, University of Zurich, 8057 Zurich, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bigler
authors.affiliation: the Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic, and.
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
authors.affiliation: From the Department of Plant and Microbial Biology, University of Zurich, 8008 Zurich, Switzerland, chringli@botinst.uzh.ch.
authors.forename: Christoph
authors.initials: C
authors.lastname: Ringli
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D044948:Flavonols / Q000378:metabolism*; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006602:Hexosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D063245:Plant Development; D012210:Rhamnose / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D044948:Flavonols; D007210:Indoleacetic Acids; D005964:Glucosyltransferases; D006602:Hexosyltransferases; C519657:RHM1 protein, Arabidopsis; C521109:UGT89C1 protein, Arabidopsis; D012210:Rhamnose
keywords: Arabidopsis thaliana; UGT89C1; auxin; auxin transport; auxin turnover; flavonoid; flavonol glycosides; flavonols; metabolism; rhamnose*; rol1-2; transport