title: Isolation, diversity and acetylcholinesterase inhibitory activity of the culturable endophytic fungi harboured in Huperzia serrata from Jinggang Mountain, China.
abstract: Huperzia serrata has many important medicinal properties with proven pharmacological potential. Some of these properties may be mediated by its endophytic fungi. To test this hypothesis, in the present study, we provided a first insights into evaluating the species composition and acetylcholinesterase (AChE) inhibitory activity of the culturable endophytic fungi of H. serrata from the regional at Jinggang Mountain in southeastern China. A total number of 885 fungal isolates distributed across 44 genera and 118 putative species were obtained from 1422 fragments of fine H. serrata roots, stems and leaves base on ITS-rDNA sequences BLAST analysis. The endophytic fungi were phylogenetically diverse and species-rich, with high rate of colonization and isolation. The assemble of endophytic fungi consisted mainly of Ascomycota (97.15%), followed by Basidiomycota (1.92%) and unknown fungal species (0.90%). Colletotrichum (64.29%), Phyllosticta (3.39%), Hypoxylon (2.81%), Xylaria (2.25%) and Nigrospora (2.04%) were the most abundant genera, whereas the remaining genera were infrequent groups. Although, roots yielded low abundance strains, the diverse and species-rich were both higher than that of stems and leaves. In addition, out of the 247 endophytic fungi strains determinated, 221 fungal extracts showed AChE inhibition activities in vitro. Among them, 22 endophytic fungi strains achieved high inhibitory activity (≥50%) on AChE which belongs to 13 genera and five incertae sedis strains. Four endophytic fungi designated as JS4 (Colletotrichum spp.), FL14 (Ascomycota spp.), FL9 (Sarcosomataceae spp.) and FL7 (Dothideomycetes spp.) were displayed highly active (≥80%) against AChE, which the inhibition effects were even more intense than the positive control. Our findings highlight that H. serrata grown in Jinggang Mountain harbors a rich and fascinating endophytic fungus community with potential AChE inhibitory activity, which could further broaden the natural acetylcholinesterase inhibitors resources used for Alzheimer's disease treatment.
authors.affiliation: Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.|Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun, 336000, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lai
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.|Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330025, China.
authors.forename: Xi-Xi
authors.initials: XX
authors.lastname: Li
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Ri-Ming
authors.initials: RM
authors.lastname: Yan
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China. zhudu12@163.com.|Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China. zhudu12@163.com.
authors.forename: Du
authors.initials: D
authors.lastname: Zhu
mesh_terms: D001203:Ascomycota / Q000302:isolation & purification; D001487:Basidiomycota / Q000302:isolation & purification / Q000502:physiology; D044822:Biodiversity; D002681:China; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification*; D020231:Colletotrichum / Q000302:isolation & purification / Q000502:physiology; D004275:DNA, Ribosomal; D060026:Endophytes / Q000302:isolation & purification / Q000502:physiology*; D032501:Huperzia / Q000201:enzymology / Q000382:microbiology*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D002800:Cholinesterase Inhibitors; D004275:DNA, Ribosomal
keywords: Acetylcholinesterase inhibitory activity; Alzheimer’s disease; Endophytic fungi; Fungal diversity; Huperzia serrata