title: Antimicrobial susceptibility of Staphylococcus pseudintermedius colonizing healthy dogs in Saskatoon, Canada.
abstract: This study reports antimicrobial susceptibility of Staphylococcus pseudintermedius carried by healthy dogs in Saskatoon, and describes changes in antimicrobial resistance since a 2008 study. One hundred healthy dogs presenting to the wellness service at the Western College of Veterinary Medicine were screened for S. pseudintermedius by culturing rectal and pharyngeal swabs. Staphylococcus pseudintermedius was identified biochemically and antimicrobial minimum inhibitory concentrations were determined by broth microdilution. Methicillin resistance was confirmed by polymerase chain reaction (PCR) and sequencing of the mecA gene. Of 221 S. pseudintermedius isolates from 78 dogs, 7 were methicillin resistant. No resistance to the fluoroquinolones, nitrofurantoin, tigecycline, vancomycin, quinupristin-dalfopristin, linezolid, or daptomycin was identified. Of the 78 positive dogs, isolates resistant to penicillin were found in 78%, to ampicillin in 61% and to tetracycline in 26%; resistance to oxacillin, erythromycin, clindamycin, trimethoprim + sulfamethoxazole, chloramphenicol, and gentamicin was found in < 10% of dogs. Compared to the 2008 study, the frequency of resistance to all drugs increased, and the frequency of colonization with pan-susceptible isolates decreased from 46% to 30%.
authors.affiliation: Department of Veterinary Microbiology (Priyantha, Rubin), Department of Small Animal Clinical Sciences (Gaunt), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Roshan
authors.initials: R
authors.lastname: Priyantha
authors.affiliation: Department of Veterinary Microbiology (Priyantha, Rubin), Department of Small Animal Clinical Sciences (Gaunt), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Mathew C
authors.initials: MC
authors.lastname: Gaunt
authors.affiliation: Department of Veterinary Microbiology (Priyantha, Rubin), Department of Small Animal Clinical Sciences (Gaunt), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Rubin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002353:Carrier State; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D024881:Drug Resistance, Bacterial; D005260:Female; D008297:Male; D012525:Saskatchewan / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents