title: Quercetin suppresses NLRP3 inflammasome activation and attenuates histopathology in a rat model of spinal cord injury.
abstract: METHODS
  Randomized experimental study.
  
  
  OBJECTIVE
  To investigate the molecular mechanisms of quercetin in spinal cord injury (SCI) rats.
  
  
  METHODS
  China.
  
  
  METHODS
  One hundred female Sprague-Dawley rats were randomly assigned into four groups: sham group, SCI group, SCI+Vehicle (Veh) group, and the SCI+Quercetin (Que) group. The influences of quercetin on proinflammatory cytokine levels, histological changes and locomotion scale were estimated.
  
  
  RESULTS
  SCI significantly promoted nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome activation and increased proinflammatory cytokine productions in the SCI group as compared with the sham group. Quercetin administration significantly decreased reactive oxygen species production, inhibited NLRP3 inflammasome activation and reduced inflammatory cytokine levels. Moreover, quercetin administration attenuated histopathology and promoted locomotion recovery.
  
  
  CONCLUSIONS
  Quercetin can attenuate tissue damage and improve neurological function recovery, and the mechanism may be related to the inhibition of NLRP3 inflammasome activation.
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, General Hospital of Beijing Military Command, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, General Hospital of Beijing Military Command, Beijing, China.
authors.forename: N
authors.initials: N
authors.lastname: Han
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: F
authors.initials: F
authors.lastname: He
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Department of Orthopedics, General Hospital of Beijing Military Command, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, General Hospital of Beijing Military Command, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008124:Locomotion / Q000187:drug effects; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics / Q000378:metabolism*; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D020127:Recovery of Function / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D000975:Antioxidants; D016207:Cytokines; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; D017382:Reactive Oxygen Species; D011794:Quercetin