title: A self-assembly peptide nanofibrous scaffold reduces inflammatory response and promotes functional recovery in a mouse model of intracerebral hemorrhage.
abstract: Self-assembly peptide nanofibrous scaffold (SAPNS), such as RADA16-I, has been shown to reduce acute brain injury and enhance functional recovery in rat intracerebral hemorrhage (ICH) models. The acidic property of RADA16-I, however, limits its application in patients. In the present study, by using a modified neutral SAPNS (the RADA16mix) in collagenase IV induced ICH mice, we detected there were less microglial and apoptotic cells in mice injected with RADA16mix, meanwhile, more cells survived in this group. In addition, behavioral tests indicated that mice treated with RADA16mix showed better functional recovery than RADA16-I. Local delivery of RADA16mix reduces acute brain injury by lowering the number of apoptotic cells, decreasing glial reaction, reducing inflammatory response and, therefore promotes functional recovery. Moreover, new nerve fibers have grown into this new SAPNS, which indicates RADA16mix is able to serve as a bridge for nerve fibers to grow through.
  
  
  
  Acute brain injury, such as intracerebral hemorrhage is a serious problem. In this work, self-assembly peptide nanofibrous scaffold (SAPNS) were tested in a rat model to aid functional recovery. Several items have been considered, such as histology, brain water content, hematoma volume, cell death and survival, inflammatory response, and nerve fiber growth. The positive data generated should pave the way towards better treatment options.
authors.affiliation: Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, PR China.
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou, PR China.
authors.forename: Liumin
authors.initials: L
authors.lastname: He
authors.affiliation: Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, PR China; Department of Human Anatomy, Medical School of Jinan University, Guangzhou, PR China; Co-innovation Center of Neuroregeneration, Nantong University, Jiangsu, PR China.
authors.forename: Libing
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, PR China; School of Biomedical Science, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, PR China; State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, PR China. Electronic address: wtwu@hku.hk.
authors.forename: Wutian
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000628:therapy*; D006801:Humans; D051379:Mice; D057139:Nanofibers; D010455:Peptides / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D054457:Tissue Scaffolds
chemical_list: D010455:Peptides; C546681:RADA16-I
keywords: Functional recovery; Inflammatory response; Intracerebral hemorrhage; Self-assembly peptide nanofibrous scaffold