title: Immune responses to Aeromonas hydrophila infection in Schizothorax prenanti fed with oxidized konjac glucomannan and its acidolysis products.
abstract: The objective of the present study was to determine the effect of dietary oxidized konjac glucomannan (OKGM) and its acidolysis products (L-OKGM) on the immune parameters and the gene expression profile of some inflammatory-related cytokines from Schizothorax prenanti during the early stages of injection with Aeromonas hydrophila. Fish were orally administered with seven different diets containing 0 g kg(-1) (control diet), 8.0, 16.0 and 32.0 g kg(-1) OKGM and L-OKGM diets for 60 days prior to injection. After 60 days, the control and the treated fish were intraperitoneally injected with 0.2 ml PBS or 2 × 10(7) cfu/ml bacteria per fish and sampled at time 6 h post-injection. The results showed that the serum lysozyme activity and complement C3 level of fish fed 8.0 g kg(-1) L-OKGM was significantly increased after bacterial infection. Moreover, the injection with A. hydrophila generally up-regulated the expression of all measured genes when compared to their corresponding controls. When compared with the control group, the expression of TLR22, TNF-α and IL-1β was significantly increased in fish fed OKGM and L-OKGM diet after bacterial injection. Furthermore, the L-OKGM diet showed higher activity to trigger the immune response against bacteria, especially the low dosage L-OKGM diet. The results suggested that both of OKGM and L-OKGM are promising feed additive for S. prenanti in aquaculture.
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, 625014 Sichuan, PR China; Life Science and Technology Institute, Yangtze Normal University, Fuling, 408100, Chongqing, PR China.
authors.forename: Qiaoran
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, PR China. Electronic address: 405135288@qq.com.
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Huailiang
authors.initials: H
authors.lastname: Xu
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, PR China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, PR China.
authors.forename: Haolan
authors.initials: H
authors.lastname: Tang
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, PR China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Feng
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D017756:Aquaculture; D003530:Cyprinidae; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007113:Immunity, Innate; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008351:Mannans / Q000737:chemistry / Q000276:immunology*; D010084:Oxidation-Reduction; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D053583:Interleukin-1beta; D008351:Mannans; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; C022901:(1-6)-alpha-glucomannan
keywords: Acidolysis products; Aeromonas hydrophila; Immune parameters; Immune-related genes; Oxidized konjac glucomannan