title: Comparative evaluation of chemical and enzymatic saccharification of mixotrophically grown de-oiled microalgal biomass for reducing sugar production.
abstract: For the commercialization of microalgal based biofuels, utilization of de-oiled carbohydrate rich biomass is important. In the present study, chemo-enzymatic hydrolysis of mixotrophically grown Scenedesmus sp. CCNM 1077 de-oiled biomass is evaluated. Among the chemical hydrolysis, use of 0.5M HCl for 45 min at 121°C resulted in highest saccharification yield of 37.87% w/w of de-oiled biomass. However, enzymatic hydrolysis using Viscozyme L at loading rate of 20 FBGU/g of de-oiled biomass, pH 5.5 and temperature 45°C for 72 h resulted in saccharification yield of 43.44% w/w of de-oiled biomass. Further, 78% ethanol production efficiency was achieved with enzymatically hydrolyzed de-oiled biomass using yeast Saccharomyces cerevisiae ATCC 6793. These findings of the present study show application of mixotrophically grown de-oiled biomass of Scenedesmus sp. CCNM 1077 as promising feedstock for bioethanol production.
authors.affiliation: Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Pancha
authors.affiliation: Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Kaumeel
authors.initials: K
authors.lastname: Chokshi
authors.affiliation: Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Rahulkumar
authors.initials: R
authors.lastname: Maurya
authors.affiliation: Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Process Design and Engineering Division, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Sourish
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Bachani
authors.affiliation: Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D056804:Biofuels / Q000032:analysis*; D018533:Biomass; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol; D005285:Fermentation; D005947:Glucose; D006868:Hydrolysis; D058086:Microalgae / Q000737:chemistry* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D044444:Scenedesmus / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D000431:Ethanol; D002480:Cellulase; D005947:Glucose
keywords: Bioethanol; Chemo-enzymatic hydrolysis; De-oiled biomass; Mixotrophic growth; Reducing sugar