title: One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces.
abstract: In this paper, we report a one-step tumor cell detection approach based on the dynamic morphological behavior tracking of cancer cells on a ligand modified surface. Every cell on the surface was tracked in real time for several minutes immediately after seeding until these were finally attached. Cancer cells were found to be very active in the aptamer microenvironment, changing their shapes rapidly from spherical to semi-elliptical, with much flatter spread and extending pseudopods at regular intervals. When incubated on a functionalized surface, the balancing forces between cell surface molecules and the surface-bound aptamers, together with the flexibility of the membranes, caused cells to show these distinct dynamic activities and variations in their morphologies. On the other hand, healthy cells remained distinguishingly inactive on the surface over the same period. The quantitative image analysis of cell morphologies provided feature vectors that were statistically distinct between normal and cancer cells.
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, TX 76010, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA.
authors.forename: Mohammed Arif I
authors.initials: MA
authors.lastname: Mahmood
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, TX 76010, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA.
authors.forename: Mohammad Raziul
authors.initials: MR
authors.lastname: Hasan
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA; Department of Biology, University of Texas at Arlington, TX 76019, USA.
authors.forename: Umair J M
authors.initials: UJ
authors.lastname: Khan
authors.affiliation: Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA; Now at Department of Chemistry, University of Idaho, Moscow, ID 83844-2343, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Allen
authors.affiliation: Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA; Department of Bioengineering, University of Texas at Arlington, TX 76011, USA; Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, TX 76010, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA; Department of Biology, University of Texas at Arlington, TX 76019, USA; Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
authors.forename: Samir M
authors.initials: SM
authors.lastname: Iqbal
keywords: Cancer Cell Morphology; Cell Imaging; Early Cancer Diagnosis; Human Glioblastoma (hGBM); Temporal Image Processing