title: FKBP8 interact with classical swine fever virus NS5A protein and promote virus RNA replication.
abstract: The non-structural 5A (NS5A) protein of classical swine fever virus (CSFV) is proven to be involved in viral replication and can also modulate cellular signaling and host cellular responses via to its ability to interact with various cellular proteins. FKBP8 is also reported to promote virus replication. Here, we show that NS5A specifically interacts with FKBP8 through coimmunoprecipitation and GST-pulldown studies. Additionally, confocal microscopy study showed that NS5A and FKBP8 colocalized in the cytoplasm. Overexpression of FKBP8 via the eukaryotic expression plasmid pDsRED N1 significantly promoted viral RNA synthesis. The cells knockdown of FKBP8 by lentivirus-mediated shRNA markedly decreased the virus replication when infected with CSFV. These data suggest that FKBP8 plays a critical role in the viral life cycle, particularly during the virus RNA replication period. The investigation of FKBP8 protein functions may be beneficial for developing new strategies to treat CSFV infection.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Helin
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Kangkang
authors.initials: K
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Tianyue
authors.initials: T
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Qizhuang
authors.initials: Q
authors.lastname: Lv
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China. zcc87051007@126.com.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000502:physiology*; D004247:DNA; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D013552:Swine; D022021:Tacrolimus Binding Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D017361:Viral Nonstructural Proteins; D004247:DNA; D022021:Tacrolimus Binding Proteins
keywords: Classical swine fever virus (CSFV); FKBP8; Interaction; NS5A