title: Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii.
abstract: BACKGROUND
  Human parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) may cause intrauterine infections with potentially severe consequences to the fetus. Current serodiagnosis of these infections is based on detection of antibodies most often by EIA and individually for each pathogen. We developed singleplex and multiplex microsphere-based Suspension Immuno Assays (SIAs) for the simultaneous detection of IgG antibodies against B19V, CMV and T. gondii.
  
  
  METHODS
  We tested the performances of SIAs as compared to in-house and commercial reference assays using serum samples from well-characterized cohorts.
  
  
  RESULTS
  The IgG SIAs for CMV and T. gondii showed good concordance with the corresponding Vidas serodiagnostics. The B19V IgG SIA detected IgG in all samples collected >10 days after onset of symptoms and showed high concordance with EIAs (in-house and Biotrin). The serodiagnostics for these three pathogens performed well in multiplex format.
  
  
  CONCLUSIONS
  We developed singleplex and multiplex IgG SIAs for the detection of anti-B19V, -CMV and -T. gondii antibodies. The SIAs were highly sensitive and specific, and had a wide dynamic range. These components thus should be suitable for construction of a multiplex test for antibody screening during pregnancy.
authors.affiliation: Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland. yilin.wang@helsinki.fi.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Helsinki University Hospital, Helsinki, Finland. lea.hedman@helsinki.fi.
authors.forename: Lea
authors.initials: L
authors.lastname: Hedman
authors.affiliation: Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland. maria.perdomo@helsinki.fi.
authors.forename: Maria F
authors.initials: MF
authors.lastname: Perdomo
authors.affiliation: Section of Clinical Virology, Department of Medical Sciences, Uppsala University and Uppsala University Hospital, Uppsala, Sweden. amalelfaitouri@hotmail.com.
authors.forename: Amal
authors.initials: A
authors.lastname: Elfaitouri
authors.affiliation: Section of Clinical Virology, Department of Medical Sciences, Uppsala University and Uppsala University Hospital, Uppsala, Sweden. agneswiener@gmail.com.
authors.forename: Agnes
authors.initials: A
authors.lastname: Bölin-Wiener
authors.affiliation: Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland. arun.q.kumar@gsk.com.|Present address: T-Cell Platform, Translational Medicine, Novartis Vaccines (a GSK company), Siena, Italy. arun.q.kumar@gsk.com.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland. Maija.Lappalainen@hus.fi.|Helsinki University Hospital, Helsinki, Finland. Maija.Lappalainen@hus.fi.
authors.forename: Maija
authors.initials: M
authors.lastname: Lappalainen
authors.affiliation: Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland. Maria.Soderlund-Venermo@helsinki.fi.
authors.forename: Maria
authors.initials: M
authors.lastname: Söderlund-Venermo
authors.affiliation: Section of Clinical Virology, Department of Medical Sciences, Uppsala University and Uppsala University Hospital, Uppsala, Sweden. jonas.blomberg@medsci.uu.se.
authors.forename: Jonas
authors.initials: J
authors.lastname: Blomberg
authors.affiliation: Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland. Klaus.Hedman@helsinki.fi.|Helsinki University Hospital, Helsinki, Finland. Klaus.Hedman@helsinki.fi.
authors.forename: Klaus
authors.initials: K
authors.lastname: Hedman
mesh_terms: D000293:Adolescent; D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008863:Microspheres; D008875:Middle Aged; D016732:Parvovirus B19, Human / Q000276:immunology*; D012698:Serologic Tests; D014122:Toxoplasma / Q000276:immunology*; D014777:Virus Diseases / Q000175:diagnosis; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M