title: Spray Dried, Pasteurised Bovine Colostrum Protects Against Gut Dysfunction and Inflammation in Preterm Pigs.
abstract: Feeding bovine colostrum (BC) improves gut maturation and function and protects against necrotizing enterocolitis, relative to formula in newborn preterm pigs. Before BC can be used for preterm infants, it is important to test if the milk processing, required to reduce bacterial load and increase shelf life, may affect bioactivity and efficacy of a BC product.
  
  
  
  We investigated if spray dried, pasteurised BC had protective effects on gut function in preterm pigs, relative to formula. After a 2-day total parenteral nutrition period, preterm pigs were fed formula for a few hours (to induce a proinflammatory state) followed by 2 days of formula (FORM, n = 14), BC (colostrum [COLOS], n = 14), spray-dried BC (POW, n = 8), or pasteurised, spray-dried BC (POWPAS, n = 9).
  
  
  
  Spray drying and pasteurisation of BC decreased the concentration of transforming growth factor-β1, -β2 and increased protein aggregation. All of the 3 BC groups had reduced necrotizing enterocolitis severity, small intestinal levels of IL-1β, -8, and colonic lactic acid levels, and increased intestinal villus height, hexose absorption, and digestive enzyme activities, relative to the FORM group (all P < 0.05). All of the 3 BC diets stimulated epithelial cell migration in a wound-healing model with IEC-6 cells.
  
  
  
  Spray drying and pasteurisation affect BC proteins, but do not reduce the trophic and anti-inflammatory effects of BC on the immature intestine. It remains to be studied if BC products will benefit preterm infants just after birth when human milk is often not available.
authors.affiliation: *National Veterinary Institute, Technical University of Denmark †Comparative Paediatrics and Nutrition, Department of Clinical Veterinary and Animal Science, University of Copenhagen, Frederiksberg ‡Department of Pediatrics and Adolescent Medicine, Rigshospitalet, Copenhagen §The Medical Research Laboratories, Department of Clinical Medicine, Faculty of Health Sciences, Aarhus University, Aarhus ¶Department of Food Science, University of Copenhagen, Frederiksberg ||Department of Animal Science, Aarhus University, Tjele, Denmark.
authors.forename: Ann Cathrine F
authors.initials: AC
authors.lastname: Sty
authors.forename: Per T
authors.initials: PT
authors.lastname: Sangild
authors.forename: Kerstin
authors.initials: K
authors.lastname: Skovgaard
authors.forename: Thomas
authors.initials: T
authors.lastname: Thymann
authors.forename: Mette
authors.initials: M
authors.lastname: Bjerre
authors.forename: Dereck E W
authors.initials: DE
authors.lastname: Chatterton
authors.forename: Stig
authors.initials: S
authors.lastname: Purup
authors.forename: Mette
authors.initials: M
authors.lastname: Boye
authors.forename: Peter M H
authors.initials: PM
authors.lastname: Heegaard
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D003126:Colostrum; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D007249:Inflammation / Q000175:diagnosis / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D061352:Pasteurization; D010539:Permeability; D013552:Swine; D014021:Tissue Preservation / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers