title: Sequencing and comparative genomics analysis inSenecio scandensBuch.-Ham. Ex D. Don, based on full-length cDNA library.
abstract: Senecio scandensBuch.-Ham. ex D. Don, an important antibacterial source of Chinese traditional medicine, has a widespread distribution in a few ecological habitats of China. We generated a full-length complementary DNA (cDNA) library from a sample of elite individuals with superior antibacterial properties, with satisfactory parameters such as library storage (4.30 × 106CFU), efficiency of titre (1.30 × 106CFU/mL), transformation efficiency (96.35%), full-length ratio (64.00%) and redundancy ratio (3.28%). The BLASTN search revealed the facile formation of counterparts between the experimental sample andArabidopsis thalianain view of high-homology cDNA sequence (90.79%) withe-values <1e- 50. Sequence similarities to known proteins indicate that the entire sequences of the full-length cDNA clones consist of the major of functional genes identified by a large set of microarray data from the present experimental material. For other Compositae species, a large set of full-length cDNA clones reported in the present article will serve as a useful resource to facilitate further research on the transferability of expressed sequence tag-derived simple sequence repeats (EST-SSR) development, comparative genomics and novel transcript profiles.
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical College , Zunyi , Guizhou , P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Qian
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical College , Zunyi , Guizhou , P.R. China.
authors.forename: Junjiao
authors.initials: J
authors.lastname: Ping
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical College , Zunyi , Guizhou , P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical College , Zunyi , Guizhou , P.R. China.
authors.forename: Delin
authors.initials: D
authors.lastname: Xu
keywords: Senecio scandens Buch.-Ham. ex D. Don; comparative genetics; expressed sequence tags (ESTs); full-length cDNA library