title: Effect of Coating Method on the Survival Rate of L. plantarum for Chicken Feed.
abstract: This study was designed to find the most suitable method and wall material for microencapsulation of the Lactobacillus plantarum to maintain cell viability in different environmental conditions. To improve the stability of L. plantarum, we developed an encapsulation system of L. plantarum, using water-in-oil emulsion system. For the encapsulation of L. plantarum, corn starch and glyceryl monostearate were selected to form gel beads. Then 10% (w/v) of starch was gelatinized by autoclaving to transit gel state, and cooled down at 60ºC and mixed with L. plantarum to encapsulate it. The encapsulated L. plantarum was tested for the tolerance of acidic conditions at different temperatures to investigate the encapsulation ability. The study indicated that the survival rate of the microencapsulated cells in starch matrix was significantly higher than that of free cells in low pH conditions with relatively higher temperature. The results showed that corn starch as a wall material and glycerol monostearate as a gelling agent in encapsulation could play a role in the viability of lactic acid bacteria in extreme conditions. Using the current study, it would be possible to formulate a new water-in-oil system as applied in the protection of L. plantarum from the gastric conditions for the encapsulation system used in chicken feed industry.
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul 143-701, Korea.
authors.forename: Sang-Yoon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Bioindustrial Technologies, Konkuk University, Seoul 143-701, Korea.
authors.forename: Yeon-Ji
authors.initials: YJ
authors.lastname: Jo
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul 143-701, Korea.
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: Department of Food Science and Biotechnology, CHA University, Sungnam 463-836, Korea.
authors.forename: Boo-Yong
authors.initials: BY
authors.lastname: Lee
authors.affiliation: MILAE Resources ML Co., LTD., Seoul 138-050, Korea.
authors.forename: Jong-Kwon
authors.initials: JK
authors.lastname: Han
authors.affiliation: Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Gyeonggi 429-793, Korea.
authors.forename: Jae Kag
authors.initials: JK
authors.lastname: Lim
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Oh
keywords: Lactobacillus plantarum; encapsulation stability; gel bead; water in oil emulsion