title: Cysteine Dioxygenase Type 1 Inhibits Osteogenesis by Regulating Wnt Signaling in Primary Mouse Bone Marrow Stromal Cells.
abstract: Mesenchymal stem cells (MSCs) are multipotent cells, which can give rise to variety of cell types, including adipocytes and osteoblasts. Previously, we have shown that cysteine dioxygenase type 1 (Cdo1) promoted adipogenesis of primary mouse bone marrow stromal cells (BMSCs) and 3T3-L1 pre-adipocytes via interaction with Pparγ. However, the role of Cdo1 in osteogenesis remains unclear. Here, we demonstrated that expression of Cdo1 was elevated during osteoblastic differentiation of BMSCs in vitro. Interestingly, knockdown of Cdo1 by siRNA led to an increased expression of osteogenic related genes, elevated alkaline phosphatase (ALP) activity, and enhanced mineralization. Overexpression of Cdo1 in BMSCs inversely suppressed the osteogenesis. Furthermore, we found that overexpression of Cdo1 impaired Wnt signaling and restricted the Wnt3a induced expression of osteogenic transcriptional factors, such as Runx2 and Dlx5. Collectively, our findings indicate Cdo1 suppresses osteogenic differentiation of BMSCs, through a potential mechanism which involves in Wnt signaling reduction concomitantly.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.|Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.|Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.|Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.|Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Zichao
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.|Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Xianglong
authors.initials: X
authors.lastname: Han
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China.|Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Bai
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, University of Maryland, School of Dentistry, Baltimore, MD, USA.
authors.forename: Eung-Kwon
authors.initials: EK
authors.lastname: Pae
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics; D050558:Cysteine Dioxygenase / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060449:Wnt Signaling Pathway
chemical_list: D015415:Biomarkers; D034741:RNA, Small Interfering; D050558:Cysteine Dioxygenase