title: Mobilisporobacter senegalensisgen. nov., sp. nov., an anaerobic bacterium isolated from tropical shea cake.
abstract: A Gram-stain positive, endospore-forming, strictly anaerobic bacterium, designated strain Gal1T, was isolated from shea cake, a waste material from the production of shea butter, originating from Saraya, Senegal. The cells were rod-shaped, slightly curved, and motile with peritrichous flagella. The strain was oxidase-negative and catalase-negative. Growth was observed at temperatures ranging from 15 to 45 °C (optimum 30 °C) and at pH 6.5-9.3 (optimum pH 7.8). The salinity range for growth was 0-3.5 % NaCl (optimum 1 %). Yeast extract was required for growth. Strain Gal1Tfermented various carbohydrates such as mannose, mannitol, arabinose, cellobiose, fructose, glucose, maltose, sucrose, trehalose and lactose and the major end-products were ethanol and acetate. The only major cellular fatty acid was C16 : 0 (19.6 %). The DNA base G+C content of strain Gal1Twas 33.8 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that this strain was related toMobilitalea sibiricaDSM 26468Twith 94.27 % similarity,Clostridium populetiATTC 35295Twith 93.94 % similarity, andClostridium aminovalericumDSM 1283TandAnaerosporobacter mobilisDSM 15930Twith 93.63 % similarity. On the basis of phenotypic characteristics, phylogenetic analysis and the results of biochemical and physiological tests, strain Gal1Twas clearly distinguished from closely related genera, and strain Gal1Tcan be assigned to a novel species of a new genus for which the nameMobilisporobacter senegalensisgen. nov., sp. nov. is proposed. The type strain is Gal1T( = DSM 26537T= JCM 18753T).
authors.affiliation: Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.|Laboratoire de Microbiologie Appliquée et de Génie Industriel, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop,BP 5005 Dakar-Fann, Dakar,Senegal.
authors.forename: Malick
authors.initials: M
authors.lastname: Mbengue
authors.affiliation: Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.|Laboratoire de Microbiologie Appliquée et de Génie Industriel, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop,BP 5005 Dakar-Fann, Dakar,Senegal.
authors.forename: Abdoulaye
authors.initials: A
authors.lastname: Thioye
authors.affiliation: Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.
authors.forename: Marc
authors.initials: M
authors.lastname: Labat
authors.affiliation: Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Casalot
authors.affiliation: Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.
authors.forename: Manon
authors.initials: M
authors.lastname: Joseph
authors.affiliation: Laboratoire de Biochimie, Faculté des Sciences et Techniques, Université Cheikh Anta Diop,BP 5005 Dakar-Fann, Dakar,Senegal.
authors.forename: Abdoulaye
authors.initials: A
authors.lastname: Samb
authors.affiliation: Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.
authors.forename: Zouhaier
authors.initials: Z
authors.lastname: Ben Ali Gam