title: Gene variants at FTO, 9p21, and 2q36.3 are age-independently associated with myocardial infarction in Czech men.
abstract: OBJECTIVE
  Cardiovascular disease (CVD) is a major cause of morbidity and mortality in developed countries. This study aimed to confirm the effect of common putative CVD-associated gene variants (FTO rs17817449, KIF6 rs20455, 9p21 rs10757274 and 2q36.3 rs2943634) on CVD manifestation, and determine whether this effect differs between younger (< 50 years) and older CVD patients.
  
  
  METHODS
  1191 controls and 1889 MI patients were analyzed. All participants were Caucasian Czech males aged <65 years (532 were <50 years) who were examined at cardiology clinics in Prague, Czech Republic. Variants of FTO, 9p21, 2q36.3, and KIF-6 were genotyped using PCR-RFLP or TaqMan assay.
  
  
  RESULTS
  Variants of FTO (OR 1.48; 95% CI, 1.19-1.84 in a TT vs. GG comparison, p=0.0005); 9p21 (OR 1.74; 95% CI, 1.41-2.14 in an AA vs. GG comparison, p=0.0001); and 2q36.3 (OR 1.34; 95%CI, 1.09-1.65 in an AA vs. +C comparison, p=0.006) were significantly associated with MI in the male Czech population. In contrast, genotype frequencies of KIF-6 (rs20455) were the same in patients and controls (P=1.00). Nearly identical results were observed when a subset of young MI patients (N=532, aged <50 years) was analyzed.
  
  
  CONCLUSIONS
  We confirmed the importance of determining FTO, 9p21, and 2q36.3 variants as part of the genetic determination of MI risk in the Czech male population.
authors.affiliation: Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: jahb@ikem.cz.
authors.forename: J A
authors.initials: JA
authors.lastname: Hubacek
authors.affiliation: 3(rd) Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic. Electronic address: vrablikm@seznam.cz.
authors.forename: M
authors.initials: M
authors.lastname: Vrablik
authors.affiliation: Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: dadl@ikem.cz.
authors.forename: D
authors.initials: D
authors.lastname: Dlouha
authors.affiliation: Cardiology Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: vlst@ikem.cz.
authors.forename: V
authors.initials: V
authors.lastname: Stanek
authors.affiliation: Cardiology Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: mage@ikem.cz.
authors.forename: M
authors.initials: M
authors.lastname: Gebauerova
authors.affiliation: Preventive Cardiology Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: vead@ikem.cz.
authors.forename: V
authors.initials: V
authors.lastname: Adamkova
authors.affiliation: 3(rd) Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic. Electronic address: richard.ceska@vfn.cz.
authors.forename: R
authors.initials: R
authors.lastname: Ceska
authors.affiliation: 2rd Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic. Electronic address: Gabriela.Dostalova@vfn.cz.
authors.forename: G
authors.initials: G
authors.lastname: Dostálová
authors.affiliation: 2rd Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic. Electronic address: alinh@lf1.cuni.cz.
authors.forename: A
authors.initials: A
authors.lastname: Linhart
authors.affiliation: Institute of Medical Chemistry and Laboratory Diagnostics and the 4(th) Department of Internal Medicine, 1st Faculty of Medicine, Charles University in Prague, Czech Republic. Electronic address: vitek@cesnet.cz.
authors.forename: L
authors.initials: L
authors.lastname: Vitek
authors.affiliation: Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: japi@ikem.cz.
authors.forename: J
authors.initials: J
authors.lastname: Pitha
mesh_terms: D000367:Age Factors; D000375:Aging; D015992:Body Mass Index; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D018153:Czech Republic; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors
keywords: Cardiovascular disease; Polymorphism; Slavic population; Young