title: Graphene quantum dots FRET based sensor for early detection of heart attack in human.
abstract: Cardiac immunosensor for early detection of heart attack (myocardial infarction) was developed using amine functionalized graphene quantum dots (afGQDs) conjugated with antibody anti-cardiac Troponin I (anti-cTnI) to detect cardiac marker antigen Troponin I (cTnI) in blood based on fluorescence resonance energy transfer (FRET) between conjugate and graphene (quencher) only in 10 min. The anti-cTnI was covalently conjugated to afGQDs through carbodiimide coupling reaction. The conjugate was characterized by zeta potential UV-vis spectroscopy and field emission scanning electron microscopy (FESEM). The sensing performance of the sensor was studied with respect to changes in the photon count and photoluminescence of GQDs based on interaction of target cTnI with its specific anti-cTnI antibody. The sensor is highly specific and shows negligible response to non-specific antigens. The sensor displayed a linear response to cTnI from 0.001 to 1000 ng mL(-1) with a limit of detection of 0.192 pg mL(-1).
authors.affiliation: CSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India; CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Bhatnagar
authors.affiliation: CSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
authors.forename: Vanish
authors.initials: V
authors.lastname: Kumar
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: ashokigib@rediffmail.com.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: CSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: inderpreety@yahoo.co.in.
authors.forename: Inderpreet
authors.initials: I
authors.lastname: Kaur
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D042241:Early Diagnosis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis*; D045663:Quantum Dots / Q000737:chemistry*; D019210:Troponin I / Q000097:blood*
chemical_list: D055910:Antibodies, Immobilized; D019210:Troponin I; D006108:Graphite
keywords: Cardiac Troponin I sensor; FRET; Graphene quantum dots; Myocardial infarction