title: Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3.
abstract: In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.
authors.forename: Marit
authors.initials: M
authors.lastname: Reitsma
authors.forename: Shanna
authors.initials: S
authors.lastname: Bastiaan-Net
authors.affiliation: Department of Food Science, University of Parma , 43124 Parma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Sforza
authors.affiliation: Department of Internal Medicine, section Allergology, ErasmusMC , 3015 CE Rotterdam, The Netherlands.
authors.forename: Johanna P M
authors.initials: JP
authors.lastname: van der Valk
authors.affiliation: Department of Internal Medicine, section Allergology, ErasmusMC , 3015 CE Rotterdam, The Netherlands.
authors.forename: Roy
authors.initials: R
authors.lastname: van Gerth van Wijk
authors.forename: Huub F J
authors.initials: HF
authors.lastname: Savelkoul
authors.affiliation: Department of Internal Medicine, section Allergology, ErasmusMC , 3015 CE Rotterdam, The Netherlands.
authors.forename: Nicolette W
authors.initials: NW
authors.lastname: de Jong
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Wichers
mesh_terms: D000595:Amino Acid Sequence; D031021:Anacardium / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D052179:Antigens, Plant / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018604:Epitope Mapping; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*
chemical_list: D052179:Antigens, Plant; D010940:Plant Proteins
keywords: Ana o 1; Ana o 2; Ana o 3; cashew; tree nut allergy