title: Genetic Instability in Lymphocytes is Associated With Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation.
abstract: Earlier reports have suggested that exposure to radiation at workplace may induce cytogenetic abnormalities. However, the association between plasma antioxidants and the cytogenetic abnormalities in these patients has not been elucidated till now. Hence, the present study was undertaken to determine the relationship between the cytogenetic abnormalities, plasma antioxidant system, and the radiation exposure levels in men who were occupationally exposed to ionizing radiation. The study included 134 male volunteers, among whom 83 were occupationally exposed to ionizing radiation. Incidence of micronuclei and chromosomal aberration was assessed in lymphocytes. Total and reduced glutathione (GSH), total antioxidant capacity (TAC), superoxide dismutase (SOD), and lipid peroxidation were assessed in the plasma. The micronuclei frequency and chromosomal aberrations were significantly higher in the exposed group in comparison to the nonexposed group (P < 0.01-0.0001). Similarly, GSH, TAC, and SOD in the blood plasma were significantly higher in the exposed group than the nonexposed group (P < 0.01-0.0001). However, the level of malondialdehyde, which is an indicator of lipid peroxidation, did not differ significantly between both the groups. Importantly, radiation absorbed dose exhibited a positive correlation with the incidence of micronuclei in blood lymphocytes but not with chromosomal aberrations. This study shows that the susceptibility of peripheral blood lymphocytes to chromosomal damage is associated with plasma antioxidant levels. Furthermore, increased levels of blood plasma GSH, TAC, and SOD in occupationally exposed individuals could be an adaptive measure in response to oxidative stress to protect somatic cell genetic integrity.
authors.affiliation: Department of Clinical Embryology, Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Dayanidhi
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Department of Clinical Embryology, Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Department of Clinical Embryology, Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Sujith Raj
authors.initials: SR
authors.lastname: Salian
authors.affiliation: Department of Clinical Embryology, Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Shubhashree
authors.initials: S
authors.lastname: Uppangala
authors.affiliation: Department of Clinical Embryology, Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Guruprasad
authors.initials: G
authors.lastname: Kalthur
authors.affiliation: Department of Radiotherapy, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Challapalli
authors.affiliation: Department of Radiotherapy and Oncology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Shrinidhi Gururajarao
authors.initials: SG
authors.lastname: Chandraguthi
authors.affiliation: Department of Obstetrics & Gynecology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Pratap
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Clinical Embryology, Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India satish.adiga@manipal.edu.
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Adiga
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000139:chemically induced*; D005978:Glutathione / Q000097:blood; D006282:Health Personnel; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000209:etiology*; D011839:Radiation, Ionizing; D013482:Superoxide Dismutase / Q000097:blood; D055815:Young Adult
chemical_list: D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: genetic instability; ionizing radiation; micronuclei; occupational radiation exposure; oxidative stress; plasma antioxidants