title: Polyomavirus JC microRNA expression after infection in vitro.
abstract: The in vitro expression of the Polyomavirus JC (JCPyV) microRNAs, JC-miRNA-3p and -5p, at early time points post-infection was investigated. The expression of the JCPyV microRNAs was monitored in hematopoietic progenitor KG-1 cells and in kidney fibroblast-like COS-7 cells transformed with SV40 after infection with a JCPyV CY archetype viral clone. The JCPyV DNA viral load was low in KG-1 cells compared with that in COS-7 cells, which showed productive viral replication. The expression of the JCPyV microRNAs was observed from 12h after the viral infection of both cell types and in the exosomes present in their cell supernatant. Additionally, this study verified that the JCPyV microRNAs in the exosomes present in the supernatants produced by the infected cells might be carried into uninfected cells. These findings suggest that additional investigations of the expression of JCPyV microRNAs and their presence in exosomes are necessary to shed light on their regulatory role during viral reactivation.
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Giovannelli
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Clausi
authors.affiliation: Department of Infectious Diseases, Kobe Institute of Health, 4-6-5 Minatojima-Nakamachi, Chuo-ku, Kobe, Japan.
authors.forename: Souichi
authors.initials: S
authors.lastname: Nukuzuma
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
authors.forename: Nunzia
authors.initials: N
authors.lastname: Della Malva
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Nosi
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. Electronic address: simone.giannecchini@unifi.it.
authors.forename: Simone
authors.initials: S
authors.lastname: Giannecchini
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis; D055354:Exosomes / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D007577:JC Virus / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D013997:Time Factors; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs; D012367:RNA, Viral
keywords: Exosomes; JCPyV; KG-1 cells; MicroRNA expression; Viral replication