title: Superresolution microscopy with transient binding.
abstract: For single-molecule localization based superresolution, the concentration of fluorescent labels has to be thinned out. This is commonly achieved by photophysically or photochemically deactivating subsets of molecules. Alternatively, apparent switching of molecules can be achieved by transient binding of fluorescent labels. Here, a diffusing dye yields bright fluorescent spots when binding to the structure of interest. As the binding interaction is weak, the labeling is reversible and the dye ligand construct diffuses back into solution. This approach of achieving superresolution by transient binding (STB) is reviewed in this manuscript. Different realizations of STB are discussed and compared to other localization-based superresolution modalities. We propose the development of labeling strategies that will make STB a highly versatile tool for superresolution microscopy at highest resolution.
authors.affiliation: Technische Universität Braunschweig, Institut für Physikalische und Theoretische Chemie - NanoBioSciences, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Molle
authors.affiliation: Technische Universität Braunschweig, Institut für Physikalische und Theoretische Chemie - NanoBioSciences, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Raab
authors.affiliation: Technische Universität Braunschweig, Institut für Physikalische und Theoretische Chemie - NanoBioSciences, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Holzmeister
authors.affiliation: Technische Universität Braunschweig, Institut für Physikalische und Theoretische Chemie - NanoBioSciences, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Schmitt-Monreal
authors.affiliation: Universität Regensburg, Institut für Biochemie, Genetik und Mikrobiologie, Lehrstuhl für Mikrobiologie, Universitätsstraße 31, 93053 Regensburg, Germany.
authors.forename: Dina
authors.initials: D
authors.lastname: Grohmann
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
authors.affiliation: Technische Universität Braunschweig, Institut für Physikalische und Theoretische Chemie - NanoBioSciences, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany. Electronic address: p.tinnefeld@tu-braunschweig.de.
authors.forename: Philip
authors.initials: P
authors.lastname: Tinnefeld
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D036103:Nanotechnology
chemical_list: D005456:Fluorescent Dyes; D004247:DNA