title: An Outer Membrane Protein Involved in the Uptake of Glucose Is Essential for Cytophaga hutchinsonii Cellulose Utilization.
abstract: Cytophaga hutchinsonii specializes in cellulose digestion by employing a collection of novel cell-associated proteins. Here, we identified a novel gene locus, CHU_1276, that is essential for C. hutchinsonii cellulose utilization. Disruption of CHU_1276 in C. hutchinsonii resulted in complete deficiency in cellulose degradation, as well as compromised assimilation of cellobiose or glucose at a low concentration. Further analysis showed that CHU_1276 was an outer membrane protein that could be induced by cellulose and low concentrations of glucose. Transcriptional profiling revealed that CHU_1276 exerted a profound effect on the genome-wide response to both glucose and Avicel and that the mutant lacking CHU_1276 displayed expression profiles very different from those of the wild-type strain under different culture conditions. Specifically, comparison of their transcriptional responses to cellulose led to the identification of a gene set potentially regulated by CHU_1276. These results suggest that CHU_1276 plays an essential role in cellulose utilization, probably by coordinating the extracellular hydrolysis of cellulose substrate with the intracellular uptake of the hydrolysis product in C. hutchinsonii.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China.|Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Haidian District, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China.
authors.forename: Tengteng
authors.initials: T
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China weifliu@sdu.edu.cn.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Liu
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003589:Cytophaga / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D018085:Regulon
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002475:Cellobiose; D002482:Cellulose; D005947:Glucose