title: Ultrasensitive detection of influenza viruses with a glycan-based impedimetric biosensor.
abstract: An ultrasensitive impedimetric glycan-based biosensor for reliable and selective detection of inactivated, but intact influenza viruses H3N2 was developed. Such glycan-based approach has a distinct advantage over antibody-based detection of influenza viruses since glycans are natural viral receptors with a possibility to selectively distinguish between potentially pathogenic influenza subtypes by the glycan-based biosensors. Build-up of the biosensor was carefully optimized with atomic force microscopy applied for visualization of the biosensor surface after binding of viruses with the topology of an individual viral particle H3N2 analyzed. The glycan biosensor could detect a glycan binding lectin with a limit of detection (LOD) of 5 aM. The biosensor was finally applied for analysis of influenza viruses H3N2 with LOD of 13 viral particles in 1 μl, what is the lowest LOD for analysis of influenza viral particles by the glycan-based device achieved so far. The biosensor could detect H3N2 viruses selectively with a sensitivity ratio of 30 over influenza viruses H7N7. The impedimetric biosensor presented here is the most sensitive glycan-based device for detection of influenza viruses and among the most sensitive antibody or aptamer based biosensor devices.
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: András
authors.initials: A
authors.lastname: Hushegyi
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Dominika
authors.initials: D
authors.lastname: Pihíková
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Tomas
authors.initials: T
authors.lastname: Bertok
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic.
authors.forename: René
authors.initials: R
authors.lastname: Kizek
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia. Electronic address: Jan.Tkac@savba.sk.
authors.forename: Jan
authors.initials: J
authors.lastname: Tkac
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D058266:Dielectric Spectroscopy / Q000379:methods*; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification*; D053126:Influenza A Virus, H7N7 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D057230:Limit of Detection; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
keywords: Biosensors; Electrochemical impedance spectroscopy; Glycans; Influenza virus; Lectins; SAMs