title: Co-utilization of glycerol and lignocellulosic hydrolysates enhances anaerobic 1,3-propanediol production by Clostridium diolis.
abstract: Anaerobic fermentation using lignocellulosic hydrolysates as co-substrates is an economically attractive method to enhance 1,3-propanediol (1,3-PD) production by increasing the conversion yield from glycerol. Lignocellulosic hydrolysates contain the mixed sugars that are primarily glucose, xylose, and arabinose. Therefore, these three individual sugars were used, separately, as co-substrates with glycerol, in 1,3-PD production by a Clostridium diolis strain DSM 15410, resulting in an 18%-28% increase in the 1,3-PD yield. Co-fermentation of the mixed sugars and glycerol obtained a higher intracellular NADH/NAD(+) ratio and increased the 1,3-PD yield by 22% relative to fermentation of glycerol alone. Thereafter, two kinds of lignocellulosic hydrolysates, corn stover hydrolysate and corncob molasses, were individually co-fermented with glycerol. The maximum 1,3-PD yield from glycerol reached 0.85 mol/mol. Fed-batch co-fermentation was also performed, improving the 1,3-PD yield (from 0.62 mol/mol to 0.82 mol/mol). These results demonstrate that the co-fermentation strategy is an efficient and economical way to produce 1,3-PD from glycerol.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.|State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.|Joint International Research Laboratory of Metabolic &Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.|Joint International Research Laboratory of Metabolic &Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.|Joint International Research Laboratory of Metabolic &Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.|Joint International Research Laboratory of Metabolic &Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, PR China.|Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D001089:Arabinose / Q000378:metabolism; D019149:Bioreactors; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006868:Hydrolysis; D007218:Industrial Microbiology; D007700:Kinetics; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D008965:Molasses / Q000382:microbiology; D009243:NAD / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D011409:Propylene Glycols; D009243:NAD; C036909:lignocellulose; C041787:1,3-propanediol; D008031:Lignin; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D005990:Glycerol