title: Cu2+-attached pumice particles embedded composite cryogels for protein purification.
abstract: In this study, chromatographic performance of Cu2+-attached pumice particles embedded to monolithic cryogels (Cu2+-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy, Dicle University , Diyarbakir , Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Alkan
authors.affiliation: b Department of Chemistry, Biochemistry Division, Aksaray University , Aksaray , Turkey.
authors.forename: Şeyda Ceylan
authors.initials: ŞC
authors.lastname: Cömert
authors.affiliation: c Environmental Department, Faculty of Engineering, Aksaray University , Aksaray , Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Gürbüz
authors.affiliation: d Department of Science Teaching, Faculty of Education, Dicle University , Diyarbakir , Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Doğru
authors.affiliation: b Department of Chemistry, Biochemistry Division, Aksaray University , Aksaray , Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Odabaşı
mesh_terms: D003300:Copper / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D006801:Humans; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*; D017640:Silicates / Q000737:chemistry*
chemical_list: D059985:Cryogels; D012709:Serum Albumin; D017640:Silicates; D003300:Copper; C005144:pumice
keywords: Composite cryogel; human serum albumin chromatography; metal chelate; particle embedding; pumice