title: Docosahexaenoic acid attenuates adipose tissue angiogenesis and insulin resistance in high fat diet-fed middle-aged mice via a sirt1-dependent mechanism.
abstract: METHODS
  Docosahexaenoic acid (DHA; C22: 6, n-3), one of PUFAs, exerts beneficial effects on inflammatory diseases, obesity and diabetes. Angiogenesis in adipose tissue has a major role in the development of obesity and its related metabolic complications. Inhibition of angiogenesis is an emerging strategy for the novel treatment for obesity. Thus, we examined the effect of DHA on angiogenesis in adipose tissues and investigated the underlying mechanisms.
  
  
  RESULTS
  In high-fat diet (HFD) fed middle-aged mice, DHA inhibited the macrophage-derived inflammation and angiogenesis in adipose tissues, reduced adipocyte size and body fat composition and improved insulin sensitivity. Moreover, DHA reversed the HFD-induced reduction of Sirt1 in adipose tissues. Interestingly, the effects of DHA were attenuated by lentivirus-mediated Sirt1 knockdown with increasing expression of markers of macrophage-derived inflammation and angiogenesis, associated with impaired insulin sensitivity.
  
  
  CONCLUSIONS
  Overall, our findings demonstrated that DHA reduced angiogenesis of adipose tissues and attenuated insulin resistance in HFD-induced obese mice via the activation of Sirt1.
authors.affiliation: Cardiovascular Research Center, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.|Department of Medicine, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, P.R. China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, P.R. China.|Department of Prosthodontics, Stomatological Hospital, College of Stomatology, Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Ru
authors.initials: R
authors.lastname: Jia
authors.affiliation: Cardiovascular Research Center, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Yao
authors.affiliation: Cardiovascular Research Center, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.
authors.forename: Yirui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Cardiovascular Research Center, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.affiliation: Cardiovascular Research Center, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.
authors.forename: Nanping
authors.initials: N
authors.lastname: Wang
mesh_terms: D017667:Adipocytes / Q000187:drug effects; D000273:Adipose Tissue / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D000367:Age Factors; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D007333:Insulin Resistance; D008297:Male; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009765:Obesity / Q000178:diet therapy / Q000209:etiology / Q000378:metabolism; D015434:Panniculitis / Q000178:diet therapy; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D020533:Angiogenesis Inhibitors; D004281:Docosahexaenoic Acids; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: Adipose tissue; Angiogenesis; DHA; High-fat diet; Sirt1