title: Effect of carbodiimide-derivatized hyaluronic acid gelatin on preventing postsurgical intra-abdominal adhesion formation and promoting healing in a rat model.
abstract: Adhesions often occur after abdominal surgery. It could cause chronic pelvic pain, intestinal obstruction, and infertility. A hydrogel biomaterial, carbodiimide-derivatized hyaluronic acid gelatin (cd-HA gelatin), has been successfully used to reduce adhesion formation after flexor tendon grafting. This study investigated the efficacy of cd-HA gelatin in preventing postsurgical peritoneal adhesions in a rat model. The surgical traumas were created on the underlying muscle of the abdominal wall and the serosal layer of the cecum. The wounds were covered with or without cd-HA gelatin. Animals were euthanized at day 14 after surgery. Adhesion formation was assessed with adhesion degree and adhesion breaking strength. The healing of abdominal wall was evaluated with biomechanical testing and histological analysis. The adhesions occurred in all rats (n = 12) without cd-HA gelatin treatment. The application of cd-HA gelatin significantly reduced the adhesion rate from 100% to 58%. The decrease of adhesion breaking strength also manifested that cd-HA gelatin could reduce postsurgical intra-abdominal adhesion formation. Moreover, it was found that cd-HA gelatin was a safe material and could promote tissue healing. The cd-HA gelatin hydrogel could reduce the formation of intra-abdominal adhesions without adversely effects on wound healing.
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Long-Xiang
authors.initials: LX
authors.lastname: Lin
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Zhang
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Sun
mesh_terms: D034861:Abdominal Wall / Q000473:pathology* / Q000601:surgery*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D002234:Carbodiimides / Q000737:chemistry / Q000627:therapeutic use; D005260:Female; D005780:Gelatin / Q000737:chemistry / Q000627:therapeutic use*; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000627:therapeutic use*; D020100:Hydrogels / Q000737:chemistry / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D013710:Tendons / Q000637:transplantation; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D002234:Carbodiimides; D020100:Hydrogels; D005780:Gelatin; D006820:Hyaluronic Acid
keywords: healing; hyaluronic acid; hydrogel; postsurgical intra-abdominal adhesion