title: A Combination of Human Embryonic Stem Cell-Derived Pancreatic Endoderm Transplant with LDHA-Repressing miRNA Can Attenuate High-Fat Diet Induced Type II Diabetes in Mice.
abstract: Type II diabetes mellitus (T2D) is a chronic metabolic disorder that results from defects in both insulin secretion and insulin action. The deficit and dysfunction of insulin secreting β-cell are signature symptom for T2D. Additionally, in pancreatic β-cell, a small group of genes which are abundantly expressed in most other tissues are highly selectively repressed. Lactate dehydrogenase A (LDHA) is one of such genes. Upregulation of LDHA is found in both human T2D and rodent T2D models. In this study, we identified a LDHA-suppressing microRNA (hsa-miR-590-3p) and used it together with human embryonic stem cell (hESC) derived pancreatic endoderm (PE) transplantation into a high-fat diet induced T2D mouse model. The procedure significantly improved glucose metabolism and other symptoms of T2D. Our findings support the potential T2D treatment using the combination of microRNA and hESC-differentiated PE cells.
authors.affiliation: Department of Endocrinology, Wuxi People's Hospital of Huishan District, 2 Zhanqian Street, Wuxi, Jiangsu 214187, China.
authors.forename: Yunya
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Shao
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D059305:Diet, High-Fat; D004707:Endoderm / Q000166:cytology / Q000637:transplantation*; D000066449:Human Embryonic Stem Cells / Q000637:transplantation*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000080864:Lactate Dehydrogenase 5; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology; D016035:Pancreas Transplantation; D033581:Stem Cell Transplantation / Q000379:methods
chemical_list: D007527:Isoenzymes; C568221:MIRN590 microRNA, human; D035683:MicroRNAs; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5