title: Chrysin alleviates allergic inflammation and airway remodeling in a murine model of chronic asthma.
abstract: Asthma is a chronic airway inflammatory disorder and progresses mainly due to airway remodeling. Chrysin, a natural flavonoid, has been reported to possess multiple biologic activities, including anti-inflammation, anti-oxidation and anti-proliferation. The present study aimed to investigate whether chrysin could relieve allergic airway inflammation and remodeling in a murine model of chronic asthma and the mechanism involved. The female BALB/c mice sensitized and challenged with ovalbumin (OVA) successfully developed airway hyperresponsiveness (AHR), inflammation and remodeling. The experimental data showed that chrysin could alleviate OVA-induced AHR. Chrysin could also reduce OVA-induced increases in the number of inflammatory cells, especially eosinophils, interleukin (IL) -4, and IL-13 in bronchoalveolar lavage fluid (BALF) and total IgE in serum. The decreased interferon-γ (IFN-γ) level in BALF was also upregulated by chrysin. In addition, inflammatory cell infiltration, goblet cell hyperplasia and the expression of α-smooth muscle actin (α-SMA) around bronchioles were suppressed by chrysin. Furthermore, the phosphorylation levels of Akt and extracellular signal-regulated kinase (ERK) could be decreased by chrysin, which are associated with airway smooth muscle cell (ASMC) proliferation. These results indicate the promising therapeutic effect of chrysin on chronic asthma, especially the progression of airway remodeling.
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing, Jiangsu 210000, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Respiratory Medicine, The First People's Hospital of Kunshan, Qianjinxi Road No. 91, Kunshan, Jiangsu 215300, PR China.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing, Jiangsu 210000, PR China.
authors.forename: Yunshi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing, Jiangsu 210000, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing, Jiangsu 210000, PR China. Electronic address: jingshuyue@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Du
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing, Jiangsu 210000, PR China. Electronic address: fenggz@njmu.edu.cn.
authors.forename: Ganzhu
authors.initials: G
authors.lastname: Feng
mesh_terms: D000109:Acetylcholine; D056151:Airway Remodeling / Q000187:drug effects / Q000276:immunology; D000485:Allergens / Q000276:immunology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000188:drug therapy / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D007073:Immunoglobulin E / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009130:Muscle, Smooth / Q000187:drug effects / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism
chemical_list: D000485:Allergens; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005419:Flavonoids; D007073:Immunoglobulin E; C043561:chrysin; D010047:Ovalbumin; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D000109:Acetylcholine
keywords: Airway hyperresponsiveness; Airway remodeling; Asthma; Chrysin; Inflammation