title: Erwinia teleogrylli sp. nov., a Bacterial Isolate Associated with a Chinese Cricket.
abstract: A bacterial isolate (SCU-B244T) was obtained in China from crickets (Teleogryllus occipitalis) living in cropland deserted for approximately 10 years. The isolated bacteria were Gram-negative, facultatively anaerobic, oxidase-negative rods. A preliminary analysis of the 16S rRNA gene sequence indicated that the strain belongs to either the genus Erwinia or Pantoea. Analysis of multilocus sequence typing based on concatenated partial atpD, gyrB and infB gene sequences and physiological and biochemical characteristics indicated that the strain belonged to the genus Erwinia, as member of a new species as it was distinct from other known Erwinia species. Further analysis of the 16S rRNA gene showed SCU-B244T to have 94.71% identity to the closest species of that genus, Erwinia oleae (DSM 23398T), which is below the threshold of 97% used to discriminate bacterial species. DNA-DNA hybridization results (5.78±2.52%) between SCU-B244T and Erwinia oleae (DSM 23398T) confirmed that SCU-B244T and Erwinia oleae (DSM 23398T) represent different species combined with average nucleotide identity values which range from 72.42% to 74.41. The DNA G+C content of SCU-B244T was 55.32 mol%, which also differs from that of Erwinia oleae (54.7 to 54.9 mol%). The polyphasic taxonomic approach used here confirmed that the strain belongs to the Erwinia group and represents a novel species. The name Erwinia teleogrylli sp. nov. is proposed for this novel taxon, for which the type strain is SCU-B244T (= CGMCC 1.12772T = DSM 28222T = KCTC 42022T).
authors.affiliation: Key laboratory of Leather Chemistry and engineering, College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, Sichuan, P. R. China.|Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key laboratory of Leather Chemistry and engineering, College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, Sichuan, P. R. China.
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Long
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, P. R. China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zeng
authors.affiliation: Key laboratory of Leather Chemistry and engineering, College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, Sichuan, P. R. China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Tian
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D002681:China; D004390:Chlorpyrifos / Q000378:metabolism / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D004885:Erwinia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006135:Gryllidae / Q000187:drug effects* / Q000382:microbiology*; D007306:Insecticides / Q000378:metabolism / Q000494:pharmacology*; D058885:Multilocus Sequence Typing; D039881:Prokaryotic Initiation Factor-2 / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007306:Insecticides; D039881:Prokaryotic Initiation Factor-2; D012336:RNA, Ribosomal, 16S; D014157:Transcription Factors; D027081:DNA Gyrase; D004390:Chlorpyrifos