title: Induced production of cytochalasans in co-culture of marine fungus Aspergillus flavipes and actinomycete Streptomyces sp.
abstract: Abstarct Secondary metabolites profiles of co-culture of Aspergillus flavipes and Streptomyces sp. that isolated from the same habitat showed an induced production of a series of cytochalasans (five aspochalasins and rosellichalasin, determined by MS and NMR analysis). These cytochalasans were found to be produced by A. flavipes in LC-MS comparison analysis, and biological activity assays revealed that they were able to cause cytotoxic effects against Streptomyces sp. within a wide range of concentrations without causing any effect to the producer A. flavipes, which favoured the producer in competition. Further induction mechanism study applying membrane-separated culture and morphology study with scanning electron microscopy (SEM) suggested that the successful induction of active secondary metabolites required microbial physical contact.
authors.affiliation: a Institute of Marine Biology & Natural Products , Ocean College, Zhejiang University , Hangzhou , China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Yu
authors.affiliation: a Institute of Marine Biology & Natural Products , Ocean College, Zhejiang University , Hangzhou , China.
authors.forename: Wanjing
authors.initials: W
authors.lastname: Ding
authors.affiliation: a Institute of Marine Biology & Natural Products , Ocean College, Zhejiang University , Hangzhou , China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Ma
mesh_terms: D001230:Aspergillus / Q000378:metabolism*; D018920:Coculture Techniques; D003572:Cytochalasins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D003572:Cytochalasins
keywords: Aspergillus flavipes; Co-culture; Streptomyces; aspochalasin; cytochalasans