title: miRNA-146a induces vascular smooth muscle cell apoptosis in a rat model of coronary heart disease via NF-κB pathway.
abstract: The aim of this study was to investigate the role of miRNA-146a in modulating the function of vascular smooth muscle cells in a rat model of coronary heart disease. Vascular smooth muscle cells were isolated and cultured from the rat coronary heart disease model and normal rats (controls). miRNA-146a levels were measured in vascular smooth muscle cells obtained from rats with coronary heart disease and control rats. The proliferation, growth, apoptosis, and activation of the NF-κB pathway in the vascular smooth muscle cells were detected using the MTT assay and flow cytometry, respectively. The role of the NF-κB pathway in modulating the apoptosis of vascular smooth muscle cells was investigated by measuring the reactivity of the cells to an NF-κB pathway inhibitor (TPCA-1). Vascular smooth muscle cells from the disease model exhibited higher levels of miRNA-146a than that by the normal controls (P = 0.0024). The vascular smooth muscle cells obtained from rats with coronary heart disease showed decreased proliferation and growth and increased apoptosis. miRNA-146a overexpression elevated the rate of cell apoptosis. The NF-κB pathway was activated in vascular smooth muscle cells obtained from rats with coronary heart disease. Inhibition of the NF- κB pathway significantly decreased the rate of vascular smooth muscle cell apoptosis in coronary heart disease rats (P = 0.0038). In conclusion, miRNA- 146a was found to induce vascular smooth muscle cell apoptosis in rats with coronary heart disease via the activation of the NF-κB signal pathway.
authors.affiliation: Department of Cardiology, Southern Medical University, Hainan Province People's Hospital, Guangzhou, Guangdong Province, China.|Department of Cardiology, Hainan Western Central Hospital, Danzhou, Hainan Province, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Hainan Western Central Hospital, Danzhou, Hainan Province, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Hainan Western Central Hospital, Danzhou, Hainan Province, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D003324:Coronary Artery Disease / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction
chemical_list: C554180:MIRN146 microRNA, rat; D035683:MicroRNAs; D016328:NF-kappa B; D053148:Caspase 3