title: Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.
abstract: Epigenetics provides a mechanism in which the environment can interact with the genotype to produce a variety of phenotypes. These epigenetic modifications have been associated with altered gene expression and silencing of repetitive elements, and these modifications can be inherited mitotically. DNA methylation is the best characterized epigenetic mark and earlier studies have examined DNA methylation profiles in peripheral blood mononuclear cells in disease. However, any disease-related signatures identified would just display differences in the relative abundance of individual cell types as each cell subset generates a unique methylation profile. Therefore is it important to identify cell- or tissue-specific changes in DNA methylation, particularly in autoimmune diseases such as type 1 diabetes.
authors.affiliation: Centre for Diabetes and Metabolic Medicine, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London, E1 2AT, UK. m.a.n.dang@qmul.ac.uk.
authors.forename: Mary N
authors.initials: MN
authors.lastname: Dang
authors.affiliation: Centre for Diabetes and Metabolic Medicine, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London, E1 2AT, UK.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Bradford
authors.affiliation: Centre for Diabetes and Metabolic Medicine, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London, E1 2AT, UK.|Department of Endocrinology and Diabetes, University Campus Bio-Medico of Rome, Via Alvaro del Portillo, 21, 00128, Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Pozzilli
authors.affiliation: Centre for Diabetes and Metabolic Medicine, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London, E1 2AT, UK.
authors.forename: R David
authors.initials: RD
authors.lastname: Leslie
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D019175:DNA Methylation; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D044127:Epigenesis, Genetic; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology
keywords: Cell isolation; DNA extraction; DNA methylation; Flow cytometry; Illumina HumanMethylation450K BeadChip; Magnetic-activated cell sorting; Type 1 diabetes