title: Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans.
abstract: 1-Deoxy-D-xylulose 5-phosphate synthase (DXS) catalyzes the initial step of the plastidial 2C-methyl-D-erythritol-4-phosphate (DOXP-MEP) pathway involved in isoprenoid biosynthesis. In this study, we cloned the complete cDNA of potato DXS gene that was designated StDXS1. StDXS1 cDNA encodes for 719 amino acid residues, with MW of 77.8 kDa, and is present in one copy in the potato genome. Phylogenetic analysis and protein sequence alignments assigned StDXS1 to a group with DXS homologues from closely related species and exhibited homodomain identity with known DXS proteins from other plant species. Late blight symptoms occurred in parallel with a reduction in StDXS1 transcript levels, which may be associated with the levels of isoprenoids that contribute to plant protection against pathogens. Subcellular localization indicated that StDXS1 targets the chloroplasts where isoprenoids are synthesized. Arabidopsis expressing StDXS1 showed a higher accumulation of carotenoids and chlorophyll as compared to wild type controls. Lower levels of ABA and GA were detected in the transgenic DXS lines as compared to control plants, which reflected on higher germination rates of the transgenic DXS lines. No changes were detected in JA or SA contents. Selected downstream genes in the DOXP-MEP pathway, especially GGPPS genes, were up-regulated in the transgenic lines.
authors.affiliation: Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada; Agriculture and Agri-Food Canada, Morden, MB, Canada.
authors.forename: Maria Antonia
authors.initials: MA
authors.lastname: Henriquez
authors.affiliation: Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada; Department of Genetics, Faculty of Agriculture, University of Tanta, Tanta, El-Gharbia, Egypt.
authors.forename: Atta
authors.initials: A
authors.lastname: Soliman
authors.affiliation: Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.
authors.forename: Genyi
authors.initials: G
authors.lastname: Li
authors.affiliation: Agriculture and Agri-Food Canada, London, Ontario, Canada.
authors.forename: Abdelali
authors.initials: A
authors.lastname: Hannoufa
authors.affiliation: Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.
authors.forename: Belay T
authors.initials: BT
authors.lastname: Ayele
authors.affiliation: Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada. Electronic address: Fouad.Daayf@umanitoba.ca.
authors.forename: Fouad
authors.initials: F
authors.lastname: Daayf
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D055750:Phytophthora infestans / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014166:Transferases / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D005936:Glucans; D010940:Plant Proteins; D015118:Eicosapentaenoic Acid; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase
keywords: Abscisic acid; Carotenoids; DOXP-MEP; DXS; Functional analysis; Jasmonic acid; Phytophthora infestans; Salicylic acid; Solanum; Terpenoids