title: Bile Salt Hydrolase (Bsh) Activity Screening of Lactobacilli: In Vitro Selection of Indigenous Lactobacillus Strains with Potential Bile Salt Hydrolysing and Cholesterol-Lowering Ability.
abstract: The bile salt hydrolase (Bsh) activity of probiotic bacterium residing in gastrointestinal tract has often being associated with its cholesterol-lowering effects. Hence, Bsh activity was explored in this study as the criterion for the selection of most potential Bsh-active and cholesterol-lowering indigenous Lactobacillus strains. Forty lactobacilli were adjudged Bsh active after a preliminary screening of 102 lactobacilli and occurrence of Bsh activity correlated well with their natural habitats. Of the 40 shortlisted lactobacilli, fifteen putative Lactobacillus strains were selected and further tested for their comparative Bsh activity. In the end, indigenous Lactobacillus plantarum strains Lp91 and Lp21 were emerged as the promising Bsh-active lactobacilli with their substrate preference inclined more towards glycocholate than other bile acid amino conjugates. In addition, strains Lp91 and Lp21 also exhibited significantly high bile salt deconjugation, cholesterol assimilation and cholesterol co-precipitation ability in vitro. In conclusion, indigenous L. plantarum strains Lp91 and Lp21 may be the promising candidate probiotics to elucidate the ecological significance of probiotic Bsh activity in vivo.
authors.affiliation: Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India. rajesh.mbu@gmail.com.|Department of Microbiology and Immunology, National Institute of Nutrition, Hyderabad, 500007, India. rajesh.mbu@gmail.com.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Grover
authors.affiliation: Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India. vkbatish@gmail.com.
authors.forename: Virender Kumar
authors.initials: VK
authors.lastname: Batish
keywords: Bile salt hydrolase; Cell-free extract; Indigenous lactobacilli