title: Silencing effect of lentiviral vectors encod-ing shRNA of Herp on endoplasmic reticulum stress and inflammatory responses in RAW 264.7 macrophages.
abstract: Herp, a mammalian protein with a ubiquitin-like domain, can be strongly upregulated by endoplasmic reticulum (ER) stress during ER-associated protein degradation. However, the other cellular functions of Herp remain unclear. We explored the effect of Herp on ER stress and inflammatory responses in RAW 264.7 macrophages that had been exposed to tunicamycin or thapsigargin. We successfully constructed recombinant lentiviral vectors for Herp short-hairpin RNA (shRNA) expression to better understand the contribution made by Herp to other signaling pathways. Western blotting revealed that the recombinant Herp lentiviral shRNA vector significantly inhibited the expression of the Herp protein in the thapsigargin-treated RAW 264.7 macrophages. The reverse transcription quantitative polymerase chain reaction results showed that knockdown Herp inhibited the expression of ER stress-related genes during exposure to tunicamycin or thapsigargin. In RAW 264.7 macrophages, knockdown Herp markedly attenuated the expression of inflammatory cytokines when exposed to tunicamycin; however, it strongly enhanced the expression of inflammatory cytokines when exposed to thapsigargin. We concluded that Herp lentiviral shRNA vectors had been successfully constructed; knockdown Herp inhibited ER stress and had a different effect on inflammatory responses in RAW 264.7 macrophages depending on whether they were exposed to tunicamycin or thapsigargin.
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: F L
authors.initials: FL
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Lei
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Nyala University, Nyala, South Darfur, Sudan.
authors.forename: T N
authors.initials: TN
authors.lastname: Mahmoud
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: P F
authors.initials: PF
authors.lastname: Lin
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Jin
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: A H
authors.initials: AH
authors.lastname: Wang
mesh_terms: D000818:Animals; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors; D007249:Inflammation / Q000235:genetics* / Q000473:pathology; D016086:Lentivirus / Q000235:genetics; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: C415829:Herpud1 protein, mouse; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D014415:Tunicamycin