title: Serum Response Factor Protects Retinal Ganglion Cells Against High-Glucose Damage.
abstract: Serum response factor (SRF), which encodes the MADS-box family of related proteins, is a common transcription factor related to the expression of genes associated with cell survival. However, SRF's role in retinal ganglion cells (RGCs) after high-glucose injury remains unclear. In this study, we investigate the protective role of SRF after high-glucose injury and its underlying mechanism. The in vitro RGC model subjected to high glucose was established by employing a 50 mmol/L glucose culture environment. As detected by real-time quantitative PCR and Western blot, SRF was significantly upregulated in RGCs treated with high glucose. Overexpression of SRF significantly promoted survival among RGCs exposed to high glucose and inhibited RGC apoptosis. Knockdown of SRF exerted an inverse effect. Moreover, SRF upregulation enhanced expression of an antioxidant protein, nuclear factor erythroid 2-related factor (Nrf2), via control of the Fos-related antigen 1 (Fra-1). SRF upregulation also affected RGC survival after high-glucose treatment. Our findings showed that overexpression of SRF promoted survival of RGCs after high-glucose injury by regulating Fra-1 and Nrf2.
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China.
authors.forename: Houcheng
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Ophthalmology, Xi'an NO.1 Hospital, No. 30 Fen Alley South Street, Xi'an, 710002, China. qiangmaqm@tom.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D005947:Glucose / Q000633:toxicity*; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism*; D026362:Serum Response Factor / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D000099169:Fos-Related Antigen 1
chemical_list: D005947:Glucose; D051267:NF-E2-Related Factor 2; D016760:Proto-Oncogene Proteins c-fos; D026362:Serum Response Factor; D000099169:Fos-Related Antigen 1; C495637:Nfe2l2 protein, rat
keywords: Fra-1; High glucose; Nrf2; Overexpression; SRF