title: TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.
abstract: RAP80 localizes to sites of DNA insults to enhance the DNA-damage responses. Here we identify TRAIP/RNF206 as a novel RAP80-interacting protein and find that TRAIP is necessary for translocation of RAP80 to DNA lesions. Depletion of TRAIP results in impaired accumulation of RAP80 and functional downstream partners, including BRCA1, at DNA lesions. Conversely, accumulation of TRAIP is normal in RAP80-depleted cells, implying that TRAIP acts upstream of RAP80 recruitment to DNA lesions. TRAIP localizes to sites of DNA damage and cells lacking TRAIP exhibit classical DNA-damage response-defect phenotypes. Biochemical analysis reveals that the N terminus of TRAIP is crucial for RAP80 interaction, while the C terminus of TRAIP is required for TRAIP localization to sites of DNA damage through a direct interaction with RNF20-RNF40. Taken together, our findings demonstrate that the novel RAP80-binding partner TRAIP regulates recruitment of the damage signalling machinery and promotes homologous recombination.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Nam
authors.initials: N
authors.lastname: Soo Lee
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Hee
authors.initials: H
authors.lastname: Jin Chung
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Hyoung-June
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon 16499, Korea.
authors.forename: Seo
authors.initials: S
authors.lastname: Yun Lee
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon 16499, Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Ji
authors.affiliation: Department of Biological Sciences, Sookmyung Women's University, Seoul 04310, Republic of Korea.
authors.forename: Yoojeong
authors.initials: Y
authors.lastname: Seo
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Seung
authors.initials: S
authors.lastname: Hun Han
authors.affiliation: Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Choi
authors.affiliation: Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
authors.forename: Miyong
authors.initials: M
authors.lastname: Yun
authors.affiliation: Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
authors.forename: Seok-Geun
authors.initials: SG
authors.lastname: Lee
authors.affiliation: Center for Genomic Integrity, Institute for Basic Science (IBS), Ulsan National Institute of Science and Technology, Ulsan 44919, Korea.
authors.forename: Kyungjae
authors.initials: K
authors.lastname: Myung
authors.affiliation: Department of Biological Sciences, Sookmyung Women's University, Seoul 04310, Republic of Korea.
authors.forename: Yonghwan
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon 16499, Korea.
authors.forename: Ho
authors.initials: H
authors.lastname: Chul Kang
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Republic of Korea.|Center for Neuroscience Imaging Research, Institute for Basic Science, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Hongtae
authors.initials: H
authors.lastname: Kim
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004249:DNA Damage / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins; D057809:HEK293 Cells; D006367:HeLa Cells; D056488:Histone Chaperones; D006801:Humans; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D046888:Tissue Array Analysis; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D009687:Nuclear Proteins; C466234:UIMC1 protein, human; C000607340:TRAIP protein, human; D044767:Ubiquitin-Protein Ligases