title: Sonicating multi-lumen sliced catheter tips after the roll-plate technique improves the detection of catheter colonization in adults.
abstract: The Maki technique is the standard method for detecting catheter tip (CT) colonization. However, some "multi-lumen" catheters finish in a vaulted fornix and end at different distances from the CT. Therefore, we compared the traditional Maki technique with the sonication method using several cross-cut fragments of the CT. Our objective was to assess the yield of the Maki technique followed by sonication in the detection of adult CT colonization and catheter-related bloodstream infection (C-RBSI). For 3 months, we prospectively performed CT cultures of polyurethane catheters from adult patients admitted to our institution. First, we performed CT culture using the Maki technique on blood agar plates and then sonicated small fragments of CTs in 5 ml of BHI followed by culture of 100 μl of the sonicate. We included a total of 252 CVCs, with overall colonization and C-RBSI rates of 14.3% (36/252) and 5.9% (15/252). Of the 36 colonized CVCs, 21 (58.3%) were detected both by Maki and sonication, 6 (16.7%) were detected only by Maki technique, and 9 (25.0%) only by sonication method. Among 15 episodes with concomitant bacteremia, both techniques were positive and concordant in 9 cases (60.0%), the result of the Maki was positive in only 1 (6.7%), and sonication in 5 (33.3%). Our study shows that both techniques are complementary. We recommend sonicating fragments of the CT from patients with bacteremia of unknown origin and a negative CT culture by the Maki technique.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain. Electronic address: mariaguembe@hotmail.com.
authors.forename: María
authors.initials: M
authors.lastname: Guembe
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Martín-Rabadán
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Cruces
authors.affiliation: CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain; Cardiac Surgery Postoperative Care Unit, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Pérez Granda
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D055499:Catheter-Related Infections / Q000175:diagnosis* / Q000382:microbiology* / Q000517:prevention & control; D002405:Catheterization, Central Venous / Q000009:adverse effects; D057785:Catheters / Q000382:microbiology*; D003141:Communicable Diseases / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D011140:Polyurethanes; D012680:Sensitivity and Specificity; D013010:Sonication / Q000379:methods*
chemical_list: D011140:Polyurethanes
keywords: Central venous catheter; Pre-treatment; Roll-plate technique; Slicing; Sonication