title: Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae.
abstract: Septins are a conserved family of GTP-binding proteins that assemble into a highly ordered array of filaments at the mother bud neck in Saccharomyces cerevisiae cells. Many molecular functions and mechanisms of the septins in S. cerevisiae were already uncovered. However, structural information is only available from modeling the crystallized subunits of the human septins into the EM cryomicroscopy data of the yeast hetero-octameric septin rod. Octameric rods are the building block of septin filaments in yeast. We present here the first crystal structure of Cdc11, the terminal subunit of the octameric rod and discuss its structure in relation to its human homologues. Size exclusion chromatography analysis revealed that Cdc11 forms homodimers through its C-terminal coiled coil tail.
authors.affiliation: Institute for Biochemistry, Albert-Ludwigs University, 79104 Freiburg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Brausemann
authors.affiliation: Institute for Biochemistry, Albert-Ludwigs University, 79104 Freiburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gerhardt
authors.affiliation: Institute for Biochemistry, Albert-Ludwigs University, 79104 Freiburg, Germany.
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Schott
authors.affiliation: Institute for Biochemistry, Albert-Ludwigs University, 79104 Freiburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Einsle
authors.affiliation: Institute of Molecular Genetics and Cell Biology, Ulm University, 89081 Ulm, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Große-Berkenbusch
authors.affiliation: Institute of Molecular Genetics and Cell Biology, Ulm University, 89081 Ulm, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Johnsson
authors.affiliation: Institute of Molecular Genetics and Cell Biology, Ulm University, 89081 Ulm, Germany. Electronic address: thomas.gronemeyer@uni-ulm.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gronemeyer
mesh_terms: D018797:Cell Cycle Proteins / Q000737:chemistry*; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D058112:Septins / Q000737:chemistry* / Q000378:metabolism
chemical_list: C099502:CDC11 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029701:Saccharomyces cerevisiae Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; C000602252:SEPTIN2 protein, human; D058112:Septins
keywords: Binding interface; Cdc11; Crystal structure; Nucleotide binding; Septins; Yeast