title: Extraction, characterization and antioxidant activities of polysaccharides of Chuanminshen violaceum.
abstract: A single factor experiment and Box-Behnken design were applied to optimize the microwave-assisted extraction (MAE) of Chuanminshen violaceum polysaccharides (CVPs). The CVPs yield was 34.59 ± 0.51%, which was in accordance with the predicted value of 35.54%, under the following optimum conditions: microwave power of 466 W, extraction temperature of 64.5 °C, extraction time of 15 min, and water-raw material ratio of 40 mL/g. Two CVPs fractions (CVPs-I and CVPs-II) were obtained via stepwise ethanol precipitation. CVPs-II was further purified using DEAE cellulose-52 chromatography to obtain the major fraction of CVPs-IIa. Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis revealed that crude CVPs, CVPs-I, and CVPs-II were neutral polysaccharides and were mainly composed of glucose and galactose. The molecular weights of CVPs-I and CVPs-IIa were 233.69 and 11.02 kDa, respectively. Furthermore, all samples exhibited certain antioxidant activity or reducing power to scavenge the DPPH and ABTS radicals, among which CVPs-II was the strongest. In conclusion, MAE is an efficient method for extracting CVPs of C. violaceum, and this plant root has the potential to be explored as a source of natural antioxidants.
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: donghmin@outlook.com.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: zhangqing@sicau.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: 15283522791@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: 765416741@qq.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: lwj18728158690@163.com.
authors.forename: Weijie
authors.initials: W
authors.lastname: Lan
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: hjingliu@163.com.
authors.forename: Jingliu
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: Lee909293@163.com.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Printing and Packaging, Wuhan University, Wuhan 430072, China. Electronic address: xiongyabo1030@163.com.
authors.forename: Yabo
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: qinwen@sicau.edu.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Qin
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D019661:Apiaceae / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D008872:Microwaves; D008970:Molecular Weight; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013451:Sulfonic Acids / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Chuanminshen violaceum; Microwave-assisted extraction; Polysaccharide