title: Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women.
abstract: Non-invasive prenatal diagnosis is used to detect the genetic material of the fetus by isolating the cell-free fetal DNA (cffDNA) from maternal peripheral blood. In order to establish an isolation method for cffDNA from maternal peripheral blood in Chinese women, the cffDNA was acquired with a two-step centrifugation using a QlAamp DNA Blood mini kit. The SRY gene of plasma DNA was amplified by polymerase chain reaction (PCR). Real-time quantitative PCR was used to measure the concentration of cffDNA in maternal peripheral blood in different pregnant women. The results of the SRY gene amplification of plasma DNA from pregnant women was the same as that of the amniocyte DNA. The average concentration of cffDNA in maternal peripheral blood of pregnant women in different gestational stages was 0.98 ng/mL (0.26-1.49 ng/mL), 1.43 ng/mL (0.46- 2.34 ng/mL), and 1.95 ng/mL (0.65-6.81 ng/mL) from early, middle, and late gestational stages, respectively. The mean of cffDNA from total DNA in plasma in different stages of gestation was 22.28% (9.86-27.81%). The lowest concentration of DNA amplified by nested-PCR in our research was 10-4-10-3 ng/μL. The isolation method for cffDNA from maternal peripheral blood was successfully established and further research into its applications will be conducted.
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.
authors.forename: W C
authors.initials: WC
authors.lastname: Yang
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.|Maternal and Child Health Care Hospital of Zigong, Zigong, Sichuan Province, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.
authors.forename: B X
authors.initials: BX
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR), China.
authors.forename: C L
authors.initials: CL
authors.lastname: Wei
authors.affiliation: Department of Biochemistry, School of Life Sciences & the State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan Province, China.
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
authors.affiliation: Institute of Reproduction and Stem Cell Engineering, Central South University Xiangya School of Medicine, Changsha, Hunan Province, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan Province, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Fu
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou City, Sichuan Province, China.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR), China.|Department of Obstetrics and Gynecology, First Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan Province, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Fu
mesh_terms: D000328:Adult; D041322:Chromosomes, Human, Y / Q000235:genetics; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D055748:SOXB1 Transcription Factors / Q000097:blood* / Q000235:genetics
chemical_list: C490906:SOX1 protein, human; D055748:SOXB1 Transcription Factors; D004247:DNA