title: miR-33a-5p modulates TNF-α-inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs.
abstract: miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p. Moreover, we show that BMP-2 induces SATB2 expression by interacting with SATB2 directly via the BMP-2-RUNX2 pathway. However, TNF-α first decreases SATB2 expression by inhibiting miR-33a-5p degradation. We thus conclude that miR-33a-5p plays a central role in this complex regulatory network. These findings will help to understand the regulatory role of miR-33a-5p in the inflammatory process.
authors.affiliation: Department of Prosthodontics, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Mi
authors.affiliation: Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Qiongling
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Prosthodontics, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, China.
authors.forename: Xipeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Functional Reconstruction, Capital Medical University School of Stomatology, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Prosthodontics, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D006801:Humans; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010012:Osteogenesis; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; C550416:MIRN33a microRNA, human; D036961:Matrix Attachment Region Binding Proteins; D035683:MicroRNAs; C493709:RUNX2 protein, human; D011994:Recombinant Proteins; C521297:SATB2 protein, human; C439524:TNF protein, human; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha
keywords: SATB2; TNF-α; hBMSCs; miR-33a-5p; osteogenesis