title: Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14.
abstract: We have probed the molecular basis of recognition between human spliceosomal U2 snRNP protein p14 and RNA targets representing the intron branch site region. Interaction of an RNA duplex representing the branch site helix perturbed at least 10 nuclear magnetic resonance cross-peaks of (15)N-labeled p14. However, similar chemical shift changes were observed upon interaction with a duplex without the bulged branch site residue, suggesting that binding of p14 to RNA is nonspecific and does not recognize the branch site. We propose that the p14-RNA interaction screens charges on the backbone of the branch site during spliceosome assembly.
authors.affiliation: Department of Chemistry & Biochemistry, Hunter College of the City University of New York , New York, New York 10065, United States.|The Graduate Group in Chemistry, The Graduate Center, City University of New York , New York, New York 10016, United States.
authors.forename: William
authors.initials: W
authors.lastname: Perea
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306, United States.
authors.forename: Kersten T
authors.initials: KT
authors.lastname: Schroeder
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306, United States.
authors.forename: Amy N
authors.initials: AN
authors.lastname: Bryant
authors.affiliation: Department of Chemistry & Biochemistry, Hunter College of the City University of New York , New York, New York 10065, United States.|The Graduate Group in Chemistry, The Graduate Center, City University of New York , New York, New York 10016, United States.
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Greenbaum
mesh_terms: D006801:Humans; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017381:Spliceosomes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D012322:RNA Precursors; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D017413:Ribonucleoprotein, U2 Small Nuclear