title: Quantitative proteomic analysis of the effects of a GalNAc/Man-specific lectin CSL on yeast cells by label-free LC-MS.
abstract: A Ca(2+)-dependent GalNAc/Man-specific lectin (CSL) from Cyclina sinensis was isolated, and its stimulatory action was characterized in yeast. CSL showed a potent effect on the production of ethanol by Saccharomyces cerevisiae. In this work, the changes in the protein expression profiles of S. cerevisiae after 24h of incubation with CSL were analyzed using label-free quantitative proteomics. A total of 1410 proteins were identified, but only 117 proteins showed significant differences in normalized volume (p<0.05). Among the latter proteins, 24 proteins were up-regulated, and 93 were down-regulated. Analysis of the proteome revealed that CSL triggered changes in the concentrations of some enzymes, such as increased expression of hexokinase, glyceraldehyde 3-phosphate dehydrogenase and enolase and decreased expression of dihydrolipoamide dehydrogenase and aldehyde dehydrogenase. These results indicate that CSL can cause some changes in the metabolic pathway involved in ethanol synthesis in S. cerevisiae. These data may help us understand the stimulatory mechanism of lectin in the fermentation process.
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University/Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Linge
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: changqingtong@dlou.edu.cn.
authors.forename: Changqing
authors.initials: C
authors.lastname: Tong
authors.affiliation: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China.
authors.forename: Qiancheng
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Science, Vladivostok 690022, Russia.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Lukyanov
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Science, Vladivostok 690022, Russia.
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Chernikov
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University/Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, PR China; College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: aisingioro@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D019295:Computational Biology / Q000379:methods; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015003:Yeasts / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D037102:Lectins; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D005947:Glucose
keywords: Cyclina sinensis; GalNAc/Man-specific lectin; Label-free; Proteomics; Saccharomyces cerevisiae