title: MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope.
abstract: The organization and function of the nuclear envelope (NE) involves hundreds of nuclear membrane proteins and myriad protein-protein interactions, most of which are still uncharacterized. Many NE proteins interact stably or dynamically with the nuclear lamina or chromosomes. This can make them difficult to extract under nondenaturing conditions, and greatly limits our ability to explore and identify functional protein interactions at the NE. This knowledge is needed to understand nuclear envelope structure and the mechanisms of human laminopathy diseases. This chapter provides detailed protocols for MCLIP (membrane cross-linking immunoprecipitation) identification of novel protein-protein interactions in mammalian cells.
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Mohammed Hakim
authors.initials: MH
authors.lastname: Jafferali
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Figueroa
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm, Sweden. Electronic address: einar.hallberg@neurochem.su.se.
authors.forename: Einar
authors.initials: E
authors.lastname: Hallberg
mesh_terms: D000818:Animals; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D041681:NIH 3T3 Cells; D009685:Nuclear Envelope / Q000737:chemistry; D009687:Nuclear Proteins / Q000302:isolation & purification* / Q000502:physiology; D025941:Protein Interaction Mapping; D046509:Tissue Culture Techniques
chemical_list: D009687:Nuclear Proteins
keywords: MCLIP; Nuclear envelope; Nuclear lamina; Nuclear membrane; Nucleus; Protein–protein interactions; Proteomics