title: A novel L-arabinose-responsive regulator discovered in the rice-blast fungus Pyricularia oryzae (Magnaporthe oryzae).
abstract: In this study we identified the L-arabinose-responsive regulator of Pyricularia oryzae that regulates L-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF), AraR, that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on L-arabinose. Deletion mutants of these genes were screened for their growth phenotype on L-arabinose. One mutant, named Δara1, was further analyzed. Our analysis demonstrated that Ara1 from P. oryzae is the functional analog of AraR from A. niger, while there is no significant sequence similarity between them.
authors.affiliation: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology, Utrecht University, The Netherlands.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Klaubauf
authors.affiliation: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology, Utrecht University, The Netherlands.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Zhou
authors.affiliation: MPA, UMR 2847 CNRS-Bayer Crop science, Lyon, France.|UMR 1290 BIOGER-CPP, INRA, AgroParisTech, Campus AgroParisTech, Ave Louis Bretignières, F75850 Thiverval-Grignon, France.
authors.forename: Marc-Henri
authors.initials: MH
authors.lastname: Lebrun
authors.affiliation: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology, Utrecht University, The Netherlands.
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
authors.affiliation: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology, Utrecht University, The Netherlands.
authors.forename: Evy
authors.initials: E
authors.lastname: Battaglia
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D020082:Magnaporthe / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012275:Oryza / Q000382:microbiology*; D010427:Pentose Phosphate Pathway; D010935:Plant Diseases / Q000382:microbiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors; D001089:Arabinose
keywords: arabinanolytic regulator; pentose catabolic pathway; rice-blast fungus