title: Tropomyosin-1 protects transformed alveolar epithelial cells against cigaret smoke extract through the stabilization of F-actin-dependent cell-cell junctions.
abstract: The aim of the study was to estimate the effect of tropomyosin-1-based structural stabilization of F-actin in transformed human alveolar epithelial line H1299 cells subjected to high oxidative stress induced by cigaret smoke extract. We demonstrated here that cigaret smoke extract induces cell shrinking and detachment as a consequence of F-actin cytoskeleton degradation in H1299 cells not overexpressing tropomyosin-1. Furthermore, the treatment of these cells with cigaret smoke extract resulted in the loss of peripheral localization of ZO-1 and initiated apoptosis. In contrast, structural stabilization of F-actin, by overexpression of tropomyosin-1, preserved cell to cell interactions through the attenuation of cortical actin organization into thin fibers and thus protected these cells against oxidative stress-induced degradation of actin cytoskeleton and cell death. In conclusion, we suggest that structural stabilization of thin cortical F-actin fibers increases link between tight junctions proteins and actin cytoskeleton and thus protects H1299 cells against cigaret smoke extract.
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Bydgoszcz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Gagat
authors.affiliation: Department and Clinic of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Bydgoszcz, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Grzanka
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Bydgoszcz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Izdebska
authors.affiliation: Department of Medicinal Chemistry, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Bydgoszcz, Poland.
authors.forename: Wiktor Dariusz
authors.initials: WD
authors.lastname: Sroka
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Bydgoszcz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Hałas-Wiśniewska
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Bydgoszcz, Poland. Electronic address: agrzanka@cm.umk.pl.
authors.forename: Alina
authors.initials: A
authors.lastname: Grzanka
mesh_terms: D000199:Actins / Q000378:metabolism*; D056809:Alveolar Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D002461:Cell Line, Transformed; D015870:Gene Expression; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D065840:Protective Factors; D055550:Protein Stability; D059748:Proteolysis; D012906:Smoke; D014026:Nicotiana / Q000737:chemistry; D014335:Tropomyosin / Q000502:physiology*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D000199:Actins; D010936:Plant Extracts; D012906:Smoke; C569042:TJP1 protein, human; C490775:TPM1 protein, human; D014335:Tropomyosin; D062826:Zonula Occludens-1 Protein
keywords: Airways epithelial cells; Chronic obstructive pulmonary disease; F-actin; Tight junctions (TJs); Tropomyosin-1; Zonula occludens (ZO)-1