title: Synthesis and immunological evaluation of N-acyl modified Tn analogues as anticancer vaccine candidates.
abstract: Tumor-associated carbohydrate antigens (TACAs), which are aberrantly expressed on the surface of tumor cells, are important targets for anticancer vaccine development. Herein, several N-acyl modified Tn analogues were synthesized and conjugated with carrier protein CRM197. The immunological results of these glycoconjugates indicated that 6-CRM197 elicited higher titers of antibodies which cross-reacted with native Tn antigen than the unmodified 2-CRM197 did. The IFN-γ-producing frequency of lymphocytes in mice treated with 6-CRM197 was obviously increased, compared to that of mice vaccinated with 2-CRM197 (p=0.016), which was typically associated with the Th1 response. Moreover, the elicited antisera against antigen 6-CRM197 reacted strongly with the Tn-positive tumor cells, implying the potential of this glycoconjugate as an anticancer vaccine.
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, PR China; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing 100191, PR China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Song
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing 100191, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing 100191, PR China.
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing 100191, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing 100191, PR China.
authors.forename: Xiu-Jing
authors.initials: XJ
authors.lastname: Zheng
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Tai
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: zhouyf383@nenu.edu.cn.
authors.forename: Yifa
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing 100191, PR China. Electronic address: xinshan@bjmu.edu.cn.
authors.forename: Xin-Shan
authors.initials: XS
authors.lastname: Ye
mesh_terms: D000215:Acylation; D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D004167:Diphtheria Toxin / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D005260:Female; D006001:Glycoconjugates / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D056724:Immunity, Humoral / Q000187:drug effects; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis*; D019169:Jurkat Cells; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D000912:Antibodies, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D001426:Bacterial Proteins; D019496:Cancer Vaccines; D004167:Diphtheria Toxin; D006001:Glycoconjugates; D007106:Immune Sera; D007074:Immunoglobulin G; C040286:Tn antigen; C028581:CRM197 (non-toxic variant of diphtheria toxin); D007371:Interferon-gamma
keywords: Anticancer; Carbohydrate; Glycoconjugate; Tn antigen; Vaccine