title: Effect of ultrafine poly(ε-caprolactone) fibers on calcium phosphate cement: in vitro degradation and in vivo regeneration.
abstract: We incorporated ultrafine polymer fibers into calcium phosphate cement (CPC) to improve the resorption rate of CPC with fiber degradation. Different weight percentages of electrospun poly(ε-caprolactone) fibers (0%, 3%, and 7%, named as ultrafine fiber-incorporated CPC0 [UFICPC0], UFICPC3, and UFICPC7) were included into preset CPC specimens for in vitro immersion in lipase phosphate-buffered solution and long-term in vivo implantation in the femoral condyle of rabbits. The effect of the ultrafine poly(ε-caprolactone) fibers with a diameter ranging from nanometer to micrometer on CPC degradation was evaluated by measuring the pH of the medium, mass loss, porosity, and physiochemical properties. For the in vivo evaluation, histomorphometrical analysis as well as three-dimensional (3D) reconstruction was applied to assess the osteogenic properties of the CPC composite. After in vitro immersion and in vivo implantation, the total porosity and macroporosity as well as the bone formation and ingrowth increased significantly during time in the fiber-incorporated CPC specimens. After 24 weeks of implantation, the degraded space was occupied by newly formed bone, and the UFICPC3 and UFICPC7 composites showed ~3.5 times higher fraction of bone volume than that of the pristine CPC (UFICPC0). In vitro and in vivo results proved that the introduction of ultrafine degradable fibers within a CPC matrix can be used to improve macroporosity efficiently and enhance CPC degradation and bone ingrowth largely.
authors.affiliation: Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Boyuan
authors.initials: B
authors.lastname: Yang
authors.affiliation: Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Limei
authors.initials: L
authors.lastname: Li
authors.affiliation: Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Man
authors.affiliation: Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002130:Calcium Phosphates / Q000737:chemistry*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D011817:Rabbits
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; C020243:calcium phosphate
keywords: bone ingrowth; calcium phosphate cement; degraded space; macroporosity; ultrafine fibers