title: Effects of glutathione on sperm quality during liquid storage in boars.
abstract: The aim of this study was to investigate the effects of different concentrations of glutathione in Modena on boar sperm quality during liquid storage at 17°C. Boar semen samples were collected and diluted with Modena containing different concentrations (0, 1, 5, 10, 15 mmol/L) of glutathione. Sperm motility, effective survival period, plasma membrane integrity, acrosome integrity, total antioxidant capacity (T-AOC) activity, malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were measured and analyzed. The results showed that Modena supplemented with 1, 5 and 10 mmol/L glutathione improved sperm motility, effective survival period, plasma membrane integrity and T-AOC, and decreased MDA content and H2O2content. Meanwhile, the semen sample diluted with Modena containing 1 mmol/L glutathione achieved optimum effect, and effective survival period was 6.1 days. After 5 days preservation, sperm motility, plasma membrane integrity and T-AOC of the group treated with 1 mmol/L glutathione were all higher than that of other groups. Meanwhile, MDA content and H2O2content were lower than that of other groups. In conclusion, Modena supplemented with glutathione decreased the oxidative stress and improved the quality of boar semen during liquid storage at 17°C, and 1 mmol/L concentration was the optimum concentration. © 2016 Japanese Society of Animal Science.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, P.R. China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, P.R. China.
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, P.R. China.
authors.forename: Gong-She
authors.initials: GS
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, P.R. China. hjh19732008@126.com.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Hu
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects*; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D013552:Swine; D013696:Temperature
chemical_list: D017382:Reactive Oxygen Species; D005978:Glutathione
keywords: antioxidant capacity; boar semen; glutathione; liquid preservation; reactive oxygen species