title: Cryomilling for the fabrication of doxorubicin-containing silica-nanoparticle/polycaprolactone nanocomposite films.
abstract: Bionanocomposites need to have a homogeneous distribution of nanomaterials in the polymeric matrix to achieve consistent mechanical and biological functions. However, a significant challenge lies in achieving the homogeneous distribution of nanomaterials, particularly through a solvent-free approach. This report introduces a technology to address this need. Specifically, cryomilling, a solvent-free, low-temperature processing method, was applied to generate a bionanocomposite film with well-dispersed nanoparticles. As a proof-of-concept, polycaprolactone (PCL) and doxorubicin-containing silica nanoparticles (Si-Dox) were processed through cryomilling and subsequently heat pressed to form the PCL/Si-Dox (cPCL/Si-Dox) film. Homogeneous distribution of Si-Dox was observed under both confocal imaging and atomic force microscopy imaging. The mechanical properties of cPCL/Si-Dox were comparable to those of the pure PCL film. Subsequent in vitro release profiles suggested that sustained release of Dox from the cPCL/Si-Dox film was achievable over 50 days. When human cervical cancer cells were seeded directly on these films, uptake of Dox was observed as early as day 1 and significant inhibition of cell growth was recorded on day 5.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore and Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Jing
authors.initials: J
authors.lastname: Lim
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Mark Seow Khoon
authors.initials: MS
authors.lastname: Chong
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Swee-Hin
authors.initials: SH
authors.lastname: Teoh
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore and NTU-Northwestern Institute of Nanomedicine, Nanyang Technological University, Singapore. cjxu@ntu.edu.sg.
authors.forename: Chenjie
authors.initials: C
authors.lastname: Xu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival; D004317:Doxorubicin / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone; D012822:Silicon Dioxide; D004317:Doxorubicin