title: Scale-up of industrial biodiesel production to 40 m(3) using a liquid lipase formulation.
abstract: In this work, we demonstrate the scale-up from an 80 L fed-batch scale to 40 m(3) along with the design of a 4 m(3) continuous process for enzymatic biodiesel production catalyzed by NS-40116 (a liquid formulation of a modified Thermomyces lanuginosus lipase). Based on the analysis of actual pilot plant data for the transesterification of used cooking oil and brown grease, we propose a method applying first order integral analysis to fed-batch data based on either the bound glycerol or free fatty acid content in the oil. This method greatly simplifies the modeling process and gives an indication of the effect of mixing at the various scales (80 L to 40 m(3) ) along with the prediction of the residence time needed to reach a desired conversion in a CSTR. Suitable process metrics reflecting commercial performance such as the reaction time, enzyme efficiency, and reactor productivity were evaluated for both the fed-batch and CSTR cases. Given similar operating conditions, the CSTR operation on average, has a reaction time which is 1.3 times greater than the fed-batch operation. We also showed how the process metrics can be used to quickly estimate the selling price of the enzyme. Assuming a biodiesel selling price of 0.6 USD/kg and a one-time use of the enzyme (0.1% (w/woil ) enzyme dosage); the enzyme can then be sold for 30 USD/kg which ensures that that the enzyme cost is not more than 5% of the biodiesel revenue. Biotechnol. Bioeng. 2016;113: 1719-1728. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs., Lyngby, Denmark.
authors.forename: Jason
authors.initials: J
authors.lastname: Price
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs., Lyngby, Denmark.
authors.forename: Mathias
authors.initials: M
authors.lastname: Nordblad
authors.affiliation: Viesel Fuel LLC, Stuart, Florida.
authors.forename: Hannah H
authors.initials: HH
authors.lastname: Martel
authors.affiliation: Viesel Fuel LLC, Stuart, Florida.
authors.forename: Brent
authors.initials: B
authors.lastname: Chrabas
authors.affiliation: Viesel Fuel LLC, Stuart, Florida.
authors.forename: Huali
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Bioenergy Opportunities, Novozymes A/S, 2880 Bagsvaerd, Denmark.
authors.forename: Per Munk
authors.initials: PM
authors.lastname: Nielsen
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs., Lyngby, Denmark. jw@kt.dtu.dk.
authors.forename: John M
authors.initials: JM
authors.lastname: Woodley
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D019149:Bioreactors; D032641:Eurotiales / Q000201:enzymology; D007218:Industrial Microbiology; D008049:Lipase / Q000378:metabolism*
chemical_list: D056804:Biofuels; D008049:Lipase
keywords: continuous bioprocess; enzymatic biodiesel; fed-batch; scale-up