title: Characterization of a Secretory Annexin in Echinococcus granulosus.
abstract: Cystic echinococcosis, caused by Echinococcus granulosus, is a widespread parasitic zoonosis causing economic loss and public health problems. Annexins are important proteins usually present in the plasma membrane, but previous studies have shown that an annexin B33 protein of E. granulosus (Eg-ANX) could be detected in the excretory/secretory products and cyst fluid. In this study, we cloned and characterized Eg-ANX. In silico analysis showed that the amino acid sequence of Eg-ANX was conserved and lacked any signal peptides. The phospholipid-binding activity of recombinant Eg-ANX (rEg-ANX) was tested; liposomes could bind to rEg-ANX only in the presence of Ca(2+). In addition, we performed western blotting and immunohistochemical analyses to further validate the secretory properties of Eg-ANX. The protein could be detected in the cyst fluid of E. granulosus and was also present in the intermediate host tissues, which suggested that Eg-ANX might play an important role in parasite-host interaction.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xingju
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China guangyou1963@aliyun.com.
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D048209:Echinococcus granulosus / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D017302:Annexins; D015801:Helminth Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger; D011994:Recombinant Proteins