title: Strain improvement of Chlorella sp. for phenol biodegradation by adaptive laboratory evolution.
abstract: Microalgae are highly efficient photosynthesis cell factories for CO2 capture, biofuel productions and wastewater treatment. Phenol is a typical environmental contaminant. Microalgae normally have a low tolerance for, and a low degradation rate to, high concentration of phenol. Adaptive laboratory evolution was performed for phenolic wastewater treatment by Chlorella sp. The resulting strain was obtained after 31 cycles (about 95d) under 500mg/L phenol as environmental stress. It could grow under 500mg/L and 700mg/L phenol without significant inhibition. The maximal biomass concentrations of the resulting strain at day 8 were 3.40g/L under 500mg/L phenol and 2.70g/L under 700mg/L phenol, respectively. They were more than two times of those of the original strain. In addition, 500mg/L phenol was fully removed by the resulting strain in 7d when the initial cell density was 0.6g/L.
authors.affiliation: CAS Key Lab of Low-Carbon Conversion Science & Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Wang
authors.affiliation: CAS Key Lab of Low-Carbon Conversion Science & Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China.
authors.forename: Chuizhao
authors.initials: C
authors.lastname: Xue
authors.affiliation: CAS Key Lab of Low-Carbon Conversion Science & Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: CAS Key Lab of Low-Carbon Conversion Science & Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China. Electronic address: zhaoqy@sari.ac.cn.
authors.forename: Quanyu
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: CAS Key Lab of Low-Carbon Conversion Science & Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: CAS Key Lab of Low-Carbon Conversion Science & Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D019143:Evolution, Molecular; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism* / Q000494:pharmacology; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry
chemical_list: D010636:Phenols; D062065:Wastewater
keywords: Adaptive laboratory evolution; Microalgae; Phenol biodegradation; Wastewater treatment