title: Bagasse hydrolyzates from Agave tequilana as substrates for succinic acid production by Actinobacillus succinogenes in batch and repeated batch reactor.
abstract: The aim of this work was to obtain fermentable sugars by enzymatic or acid hydrolyses of Agave tequilana Weber bagasse in order to produce succinic acid with Actinobacillus succinogenes. Hydrolyses were carried out with mineral acids (sulfuric and hydrochloric acids) or a commercial cellulolytic enzyme, and were optimized statistically by a response surface methodology, having as factors the concentration of acid/enzyme and time of hydrolysis. The concentration of sugars obtained at optimal conditions for each hydrolysis were 21.7, 22.4y 19.8g/L for H2SO4, HCl and the enzymatic preparation respectively. Concerning succinic acid production, the enzymatic hydrolyzates resulted in the highest yield (0.446g/g) and productivity (0.57g/Lh) using A. succinogenes in a batch reactor system. Repeated batch fermentation with immobilized A. succinogenes in agar and with the enzymatic hydrolyzates resulted in a maximum concentration of succinic acid of 33.6g/L from 87.2g/L monosaccharides after 5 cycles in 40h, obtaining a productivity of 1.32g/Lh.
authors.affiliation: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico. Electronic address: rcoronagonzalez1@gmail.com.
authors.forename: Rosa Isela
authors.initials: RI
authors.lastname: Corona-González
authors.affiliation: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico.
authors.forename: Karla María
authors.initials: KM
authors.lastname: Varela-Almanza
authors.affiliation: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Arriola-Guevara
authors.affiliation: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico.
authors.forename: Álvaro de Jesús
authors.initials: Áde J
authors.lastname: Martínez-Gómez
authors.affiliation: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pelayo-Ortiz
authors.affiliation: Departamento de Madera Celulosa y Papel, Universidad de Guadalajara, km. 15.5 carretera Guadalajara-Nogales, C.P. 45020, Las Agujas, Zapopan, Jalisco, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Toriz
mesh_terms: D000143:Acids; D000188:Actinobacillus / Q000378:metabolism*; D000362:Agar; D027882:Agave; D019149:Bioreactors; D002241:Carbohydrates; D002482:Cellulose; D005285:Fermentation; D006868:Hydrolysis; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D000143:Acids; D002241:Carbohydrates; D000362:Agar; D002482:Cellulose; C027433:bagasse; D019802:Succinic Acid
keywords: Acid hydrolyzates; Actinobacillus succinogenes; Enzymatic hydrolyzates; Repeated batch fermentations; Succinic acid