title: Characterisation of IncA/C2 plasmids carrying an In416-like integron with the blaVIM-19 gene from Klebsiella pneumoniae ST383 of Greek origin.
abstract: The complete nucleotide sequences of three multidrug resistance (MDR) IncA/C-like plasmids from Enterobacteriaceae isolates carrying the VIM-type carbapenemase-encoding integrons In4863 (blaVIM-19-aacA7-dfrA1-ΔaadA1-smr2) or In4873 (blaVIM-1-aacA7-dfrA1-ΔaadA1-smr2) were determined, which are the first In416-like elements identified in Greece. Plasmids pKP-Gr642 and pKP-Gr8143 were from Klebsiella pneumoniae ST383 isolates, whereas plasmid pEcl-Gr4873 was from an Enterobacter cloacae ST88 isolate. Sequencing showed that pKP-Gr642 (162787bp) and pKP-Gr8143 (154395bp) consisted of the type 1 IncA/C2 conserved backbone, the blaCMY-2-like gene-containing region, and the ARI-B (with the sul2 gene) and ARI-A (with a class 1 integron) resistance islands, like the plasmid pUMNK88_161 from the USA. The third plasmid, pEcl-Gr4873 (153958bp), exhibited extensive similarity with the type 2 IncA/C2 plasmid pR55 from France. pEcl-Gr4873 carried only one resistance island of a hybrid transposon structure inserted in a different location to ARI-A in type 1 A/C2 plasmids. In all three plasmids, the In416-like integrons In4863 or In4873 were identified within non-identical class II transposon structures. All three In416-like-carrying regions presented significant similarities with the MDR region of the IncA/C2 plasmid pCC416 from Italy, carrying the prototype In416 integron (blaVIM-4-aacA7-dfrA1-ΔaadA1-smr2). These findings provided the basis for speculations regarding the evolution of IncA/C2 plasmids with In416-like integrons, and confirmed the rapid evolution of some IncA/C2 plasmid lineages. Considering the broad host range of IncA/C2 molecules, it seems that pKP-Gr642, pKP-Gr8143 and pEcl-Gr4873 plasmids might support the diffusion of In416-like integrons among Enterobacteriaceae.
authors.affiliation: Faculty of Medicine and University Hospital in Plzeň, Charles University in Prague, Plzeň, Czech Republic; Biomedical Center, Faculty of Medicine and University Hospital in Plzeň, Charles University in Prague, Plzeň, Czech Republic. Electronic address: c.papagiannitsis@gmail.com.
authors.forename: Costas C
authors.initials: CC
authors.lastname: Papagiannitsis
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic; CEITEC, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Dolejska
authors.affiliation: National Medicines Institute, Warsaw, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Izdebski
authors.affiliation: Department of Microbiology, National School of Public Health, Athens, Greece.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Giakkoupi
authors.affiliation: Faculty of Medicine and University Hospital in Plzeň, Charles University in Prague, Plzeň, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Skálová
authors.affiliation: Faculty of Medicine and University Hospital in Plzeň, Charles University in Prague, Plzeň, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Chudějová
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic; CEITEC, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Dobiasova
authors.affiliation: Department of Microbiology, National School of Public Health, Athens, Greece.
authors.forename: Alkiviadis C
authors.initials: AC
authors.lastname: Vatopoulos
authors.affiliation: University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Lennie P G
authors.initials: LP
authors.lastname: Derde
authors.affiliation: University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Marc J M
authors.initials: MJ
authors.lastname: Bonten
authors.affiliation: National Medicines Institute, Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Gniadkowski
authors.affiliation: Faculty of Medicine and University Hospital in Plzeň, Charles University in Prague, Plzeň, Czech Republic; Biomedical Center, Faculty of Medicine and University Hospital in Plzeň, Charles University in Prague, Plzeň, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Hrabák
mesh_terms: D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D023061:Gene Order; D006115:Greece; D006801:Humans; D032023:Integrons; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases
keywords: CMY; Class 1 integron; IncA/C(2) plasmids; VIM