title: Preparative separation of polyphenols from water-soluble fraction of Chinese propolis using macroporous absorptive resin coupled with preparative high performance liquid chromatography.
abstract: In this study, a preparative separation method was developed for isolation of eleven polyphenols from water-soluble fraction of Chinese propolis using macroporous absorptive resin (MAR) coupled with preparative high performance liquid chromatography (PHPLC). Water-soluble fraction of Chinese propolis was first "prefractioned" using MAR, which yielded four subfractions. The four subfractions were then isolated by PHPLC with an isocratic elution of methanol-water. Finally, eleven polyphenols were purified from Chinese propolis including caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxy cinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin and galangin. The purities of the compounds were determined by HPLC and the chemical structures were confirmed by UV and NMR analysis. The method developed was simple, effective, rapid, scalable and economical, and it was a promising basis for large-scale preparation of multiple components from natural products.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China. Electronic address: lxpsdta2001@sina.com.
authors.forename: Aifeng
authors.initials: A
authors.lastname: Li
authors.affiliation: College of Life Science, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Xuan
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Renmin
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Cui
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D011429:Propolis / Q000737:chemistry*
chemical_list: D059808:Polyphenols; D011429:Propolis
keywords: Chinese propolis; Macroporous absorptive resin; Polyphenols; Preparative high performance liquid chromatography; Water-soluble fraction