title: Decreased IDE and IGF2 expression but increased Aβ40 in the cerebral cortex of mouse pups by early life lead exposure.
abstract: As the abbreviation of plumbum and a chemical symbol for lead, Pb produces neurotoxic effects, which result into an impairment of learning and memory and other neurological dysfunctions. However, the mechanism of neurotoxicity of Pb exposure is unclear. The present study was undertaken to investigate the effects of maternal lead exposure on expression of insulin-degrading enzyme (IDE),insulin-like growth factor 2 (IGF2) and beta amyloid protein 40 (Aβ40) in the cerebral cortex of mice offspring. Lead exposure initiated from beginning of gestation to weaning. Lead acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.2% and 0.5% groups respectively. On the 21st postnatal day, On the PND21, the learning and memory ability were tested by water maze test and the Pb levels were also determined by graphite furnace atomic absorption spectrometry. The expression of IDE, IGF2 and Aβ40 in cerebral cortex was examined by immunohistochemistry, immunofluorescence and western blotting. The lead levels in blood and cerebral cortex of all lead exposure groups were significantly higher than that of the control group (P<0.05). In water maze test, the performances of 0.5% and 1% lead exposure groups were worse than that of the control group (P<0.05).The expression of IDE and IGF2 was decreased, but Aβ40 was increased in lead exposed groups than that of the control group (P<0.05). The decreased expression of IDE and IGF2 and increased expression of Aβ40 in the cerebral cortex of pups may contribute to the neurotoxicity associated with maternal Pb exposure.
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Henan Medical college,451191, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agriculture University, 450002, China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Mingwu
authors.initials: M
authors.lastname: Qiao
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Pingan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Shao
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agriculture University, 450002, China. Electronic address: haubiochem@163.com.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Yang
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007339:Insulysin / Q000378:metabolism*; D007854:Lead / Q000633:toxicity*; D007859:Learning Disabilities / Q000209:etiology; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology
chemical_list: D016229:Amyloid beta-Peptides; C513121:IGF2 protein, mouse; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D007854:Lead; D007335:Insulin-Like Growth Factor II; D007339:Insulysin
keywords: Beta amyloid protein 40; Cerebral cortex; Insulin-degrading enzyme; Insulin-like growth factor 2; Lead; Learning and memory