title: Sexual interactions with unfamiliar females reduce hippocampal neurogenesis among adult male rats.
abstract: Recent experiments have shown that sexual interactions prior to cell proliferation cause an increase in neurogenesis in adult male rats. Because adult neurogenesis is critical for some forms of memory, we hypothesized that sexually induced changes in neurogenesis may be involved in mate recognition. Sexually naive adult male rats were either exposed repeatedly to the same sexual partner (familiar group) or to a series of novel sexual partners (unfamiliar group), while control males never engaged in sexual interactions. Ovariectomized female rats were induced into estrus every four days. Males were given two injections of 5-bromo-2'-deoxyuridine (BrdU) (200mg/kg) to label proliferating cells, and the first sexual interactions occurred three days later. Males in the familiar and unfamiliar groups engaged in four, 30-min sexual interactions at four-day intervals, and brain tissue was collected the day after the last sexual interaction. Immunohistochemistry followed by microscopy was used to quantify BrdU-labeled cells. Sexual interactions with unfamiliar females caused a significant reduction in neurogenesis in the dentate gyrus compared to males that interacted with familiar females and compared to the control group. The familiar group showed no difference in neurogenesis compared to the control group. Males in the familiar group engaged in significantly more sexual behavior (ejaculations and intromissions) than did males in the unfamiliar group, suggesting that level of sexual activity may influence neurogenesis levels. In a second experiment, we tested whether this effect was unique to sexual interactions by replicating the entire procedure using anestrus females. We found that interactions with unfamiliar anestrus females reduced neurogenesis relative to the other groups, but this effect was not statistically significant. In combination, these results indicate that interactions with unfamiliar females reduce adult neurogenesis and the effect is stronger for sexual interactions than for social interactions.
authors.affiliation: Department of Biology, Middlebury College, McCardell Bicentennial Hall, Middlebury, VT 05753, USA; Program in Neuroscience, Middlebury College, McCardell Bicentennial Hall, Middlebury, VT 05753, USA. Electronic address: mspritze@middlebury.edu.
authors.forename: M D
authors.initials: MD
authors.lastname: Spritzer
authors.affiliation: Program in Neuroscience, Middlebury College, McCardell Bicentennial Hall, Middlebury, VT 05753, USA. Electronic address: molly.gail.curtis@gmail.com.
authors.forename: M G
authors.initials: MG
authors.lastname: Curtis
authors.affiliation: Department of Biology, Middlebury College, McCardell Bicentennial Hall, Middlebury, VT 05753, USA. Electronic address: julia.p.stern@gmail.com.
authors.forename: J P
authors.initials: JP
authors.lastname: DeLoach
authors.affiliation: Program in Neuroscience, Middlebury College, McCardell Bicentennial Hall, Middlebury, VT 05753, USA. Electronic address: jemaher00@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Maher
authors.affiliation: Program in Neuroscience, Middlebury College, McCardell Bicentennial Hall, Middlebury, VT 05753, USA. Electronic address: leanne.shulman@vermont.gov.
authors.forename: L M
authors.initials: LM
authors.lastname: Shulman
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D018891:Dentate Gyrus / Q000502:physiology; D005260:Female; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008568:Memory / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D020318:Rats, Long-Evans; D012727:Sex Characteristics; D012725:Sexual Behavior / Q000502:physiology*
keywords: adult neurogenesis; dentate gyrus; familiarity; hippocampus; sexual behavior; social behavior