title: Comparative analysis of axonal transport markers in primary mammalian neurons.
abstract: Axonal transport is important for neuronal development and the maintenance of effective neuronal function in mature cells. Observing the active transport of organelles and vesicles along the axons of living neurons has emerged as a valuable tool for probing the health of the neuron, and assessing changes associated with stress and neurodegenerative disease. Transport relies on two families of motor proteins: kinesins and dynein. Using these motors, a diverse set of cargos are transported toward the axon tip, the cell body, or anywhere in between. Of particular interest are organelles and cargos associated with disease and the changes in motility that these cargos undergo during pathogenesis. Here, we describe the factors that should be considered when studying different cargos, and the imaging parameters associated with optimal tracking of various organelles and proteins. Ultimately, the ideal cargo to investigate depends on the question being asked and the limitations of individual microscopes available for imaging.
authors.affiliation: Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA; Neuroscience Graduate Group, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Klinman
authors.affiliation: Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA; Neuroscience Graduate Group, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
authors.forename: Erika L F
authors.initials: EL
authors.lastname: Holzbaur
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D001369:Axons / Q000378:metabolism*; D002478:Cells, Cultured; D056445:Cytoplasmic Dyneins / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism; D007734:Kymography / Q000379:methods; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats; D020813:Receptor, trkB / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
chemical_list: D052118:Lysosomal-Associated Membrane Protein 1; D008869:Microtubule-Associated Proteins; D000090802:rab7 GTP-Binding Proteins; D020813:Receptor, trkB; D056445:Cytoplasmic Dyneins; D016547:Kinesins; D020691:rab GTP-Binding Proteins
keywords: APP; Autophagosomes; Axonal transport; Live-cell imaging; Lysosomes; Mitochondria; Signaling endosomes