title: The further characterization of the peptide specifically binding to gastric cancer.
abstract: Targeting peptide has been considered to be useful as a small molecule probe leading to multifunctional properties for both imaging detection and targeting therapy. Thus, the identification of novel targets is urgently needed to develop innovative agents to effectively control gastric cancer metastasis and progression. Previously, we reported a novel 12-mer peptide, GP-5 (IHKDKNAPSLVP), binding to gastric carcinoma (GC) cells specifically and sensitively, and it was screened by using a phage displayed peptide library and primarily analyzed. In this study, it was further identified via fluorescence microscopy, flow cytometry, tissue chip and other methods. Our results indicated that the peptide GP-5 presents a particularly high affinity and specificity to GC cells and tissues, whereas only background detection occurred with other control cancer cells, cancer tissues or normal tissues. Taken together, all results support that the peptide GP-5 is a potential candidate to be developed as a useful molecule fragment for the imaging detection and targeting therapy of GC.
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Han
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC 3216, Australia.
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Fenghuei
authors.initials: F
authors.lastname: Lin
authors.affiliation: Center of Medical Nanomaterial, Yulin Normal College, Yulin, Guangxi 537000, China.
authors.forename: Guochao
authors.initials: G
authors.lastname: Nie
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Affiliated High School, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.
authors.forename: Yingzhuo
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China. Electronic address: ychhou@snnu.edu.cn.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000328:Adult; D000368:Aged; D016923:Cell Death; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D013274:Stomach Neoplasms / Q000378:metabolism*; D046888:Tissue Array Analysis
chemical_list: D010455:Peptides
keywords: Gastric carcinoma; Molecular imaging diagnosis; Phage display peptide library; Targeting peptide; Targeting therapy