title: Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.
abstract: Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correct lipid composition during sperm development and show that phosphatidic acid levels are crucial in late stages of spermatogenesis.
authors.affiliation: Department of Genetics, University of Szeged, Szeged, Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Laurinyecz
authors.affiliation: Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Péter
authors.affiliation: Department of Genetics, University of Szeged, Szeged, Hungary.
authors.forename: Viktor
authors.initials: V
authors.lastname: Vedelek
authors.affiliation: Department of Anatomy, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Attila L
authors.initials: AL
authors.lastname: Kovács
authors.affiliation: Department of Anatomy, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Juhász
authors.affiliation: Department of Genetics, University of Szeged, Szeged, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Maróy
authors.affiliation: Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Vígh
authors.affiliation: Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary baloghg@brc.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Balogh
authors.affiliation: Department of Genetics, University of Szeged, Szeged, Hungary rsinka@bio.u-szeged.hu.
authors.forename: Rita
authors.initials: R
authors.lastname: Sinka
mesh_terms: D000483:Alleles; D000818:Animals; D002798:Diacylglycerol Cholinephosphotransferase; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000502:physiology*; D017344:Genes, Insect; D007248:Infertility, Male / Q000201:enzymology* / Q000473:pathology; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry*; D008297:Male; D008928:Mitochondria; D009154:Mutation / Q000235:genetics; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010770:Phosphotransferases; D013087:Spermatids / Q000378:metabolism / Q000648:ultrastructure; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008055:Lipids; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; C000611376:Cds protein, Drosophila; D010770:Phosphotransferases; D009713:Nucleotidyltransferases; D002798:Diacylglycerol Cholinephosphotransferase
keywords: Drosophila; lipid; spermatogenesis