title: Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.
abstract: A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying the formation of chromatin structure and the function of subtelomeres, the telomere-adjacent regions, remain obscure. Here we report the roles of the conserved centromeric protein Shugoshin 2 (Sgo2) in defining chromatin structure and functions of the subtelomeres in the fission yeast Schizosaccharomyces pombe. We show that Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body 'knob'. Furthermore, the absence of Sgo2 leads to the derepression of the subtelomeric genes and premature DNA replication at the subtelomeric late origins. Thus, the subtelomeric specialized chromatin domain organized by Sgo2 represses both transcription and replication to ensure proper gene expression and replication timing.
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Sanki
authors.initials: S
authors.lastname: Tashiro
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Handa
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.|Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsuda
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Takuto
authors.initials: T
authors.lastname: Ban
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takigawa
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Miyasato
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Kojiro
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Life Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Kugou
authors.affiliation: Department of Life Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Ohta
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.|Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yasushi
authors.identifier: http://orcid.org/0000-0001-9407-8228
authors.initials: Y
authors.lastname: Hiraoka
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Masukata
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Kanoh
mesh_terms: D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D042522:DNA Replication Timing; D016195:G2 Phase; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000737:chemistry / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D029702:Schizosaccharomyces pombe Proteins; C485974:Sgo2 protein, S pombe