title: Tissue Discs: A 3D Model for Assessing Modulation of Tissue Oxygenation.
abstract: The presence of hypoxia in solid tumours is correlated with poor treatment outcome. We have developed a 3-D tissue engineered construct to quantitatively monitor oxygen penetration through tumour tissue using the exogenous 2-nitroimidazole bioreductive probe pimonidazole and phosphorescence quenching technologies. Using this in vitro model we were able to examine the influence of the biguanides metformin and phenformin, antimycin A and KCN, on the distribution and kinetics of oxygen delivery as prototypes of modulators of oxygen metabolism.
authors.affiliation: Department of Integrative Oncology, BC Cancer Research Centre, Vancouver, BC, Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Gandolfo
authors.affiliation: Department of Integrative Oncology, BC Cancer Research Centre, Vancouver, BC, Canada.
authors.forename: A H
authors.initials: AH
authors.lastname: Kyle
authors.affiliation: Department of Integrative Oncology, BC Cancer Research Centre, Vancouver, BC, Canada. aim@bccrc.ca.
authors.forename: A I
authors.initials: AI
authors.lastname: Minchinton
mesh_terms: D000968:Antimycin A / Q000494:pharmacology; D015687:Cell Hypoxia; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology; D046509:Tissue Culture Techniques; D023822:Tissue Engineering
chemical_list: D000968:Antimycin A; D011190:Potassium Cyanide; D010100:Oxygen
keywords: Hypoxia; Metabolism; Oxygen sensing; Radiosensitivity; Tumour