title: Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death.
abstract: The R2R3-MYB proteins comprise one of the largest families of transcription factors in plants. Although genome-wide analysis of this family has been carried out in some plant species, little is known about R2R3-MYB genes in canola (Brassica napus L.). In this study, we have identified 76 R2R3-MYB genes in the canola genome through mining of expressed sequence tags (ESTs). The cDNA sequences of 44 MYB genes were successfully cloned. The transcriptional activities of BnaMYB proteins encoded by these genes were assayed in yeast. The subcellular localizations of representative R2R3-MYB proteins were investigated through GFP fusion. Besides, the transcript abundance level analysis during abiotic conditions and ABA treatment identified a group of R2R3-MYB genes that responded to one or more treatments. Furthermore, we identified a previously functionally unknown MYB gene-BnaMYB78, which modulates reactive oxygen species (ROS)-dependent cell death in Nicotiana benthamiana, through regulating the transcription of a few ROS- and defence-related genes. Taken together, this study has provided a solid foundation for understanding the roles and regulatory mechanism of canola R2R3-MYB genes.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Bisi
authors.initials: B
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Wu-Zhen
authors.initials: WZ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Jingxiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Jieyu
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China jiangyq@nwafu.edu.cn.
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Jiang
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016923:Cell Death; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D020570:Genes, myb / Q000235:genetics*; D005810:Multigene Family; D018384:Oxidative Stress; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017421:Sequence Analysis; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors
keywords: Brassica napus; MYB; ROS; abiotic stress; cell death