title: Hymenobacter paludissp. nov., isolated from a marsh.
abstract: A bacterial strain, designated KBP-30T, was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized taxonomically using a polyphasic approach. Cells of strain KBP-30Twere Gram-stain-negative, aerobic, motile by gliding rods that were covered by large capsules and formed red colonies. Growth occurred at 10-37 °C (optimum 20 °C), at pH 6-8 (optimum pH 6) and with 0-1 % NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-30Tbelonged to the genusHymenobacterand was most closely related toHymenobacter ocellatusMyx 2105Twith a sequence similarity of 97.7 %; 16S rRNA gene sequence similarities were less than 95.1 % with other members of the genus. Strain KBP-30Tcontained iso-C15 : 0, summed feature 4 (iso-C17 : 1I and/or anteiso-C17 : 1B), anteiso-C15 : 0, iso-C17 : 03-OH and summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified aminolipid, an unidentified glycolipid and eight unidentified lipids. The major polyamine was homospermidine. The DNA G+C content of the genomic DNA was 60.3 mol%. The DNA-DNA relatedness of strain KBP-30Twith respect toHymenobacter ocellatusMyx 2105Twas less than 42 %. On the basis of the phylogenetic inference and phenotypic data, strain KBP-30Trepresents a novel species of the genusHymenobacter, for which the nameHymenobacter paludissp. nov. is proposed. The type strain is KBP-30T( = LMG 27293T= KCTC 32237T).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Zih-Han
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu