title: Under fungal attack on a metalliferous soil: ROS or not ROS? Insights from Silene paradoxa L. growing under copper stress.
abstract: We investigated how the adaptation to metalliferous environments can influence the plant response to biotic stress. In a metallicolous and a non-metallicolous population of Silene paradoxa the induction of oxidative stress and the production of callose and volatiles were evaluated in the presence of copper and of the PAMP fungal protein cerato-platanin, separately and in combination. Our results showed incompatibility between the ordinary ROS-mediated response to fungal attack and the acquired mechanisms of preventing oxidative stress in the tolerant population. A similar situation was also demonstrated by the sensitive population growing in the presence of copper but, in this case, with a lack of certain responses, such as callose production. In addition, in terms of the joint behaviour of emitted volatiles, multivariate statistics showed that not only did the populations respond differently to the presence of copper or biotic stress, but also that the biotic and abiotic stresses interacted in different ways in the two populations. Our results demonstrated that the same incompatibility of hyperaccumulators in ROS-mediated biotic stress signals also seemed to be exhibited by the excluder metallophyte, but without the advantage of being able to rely on the elemental defence for plant protection from natural enemies.
authors.affiliation: Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019, Sesto Fiorentino, Italy. Electronic address: cosimo.taiti@unifi.it.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Taiti
authors.affiliation: Department of Biology, Università di Firenze, via Micheli 1, 50121, Firenze, Italy. Electronic address: elisabetta.giorni@unifi.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Giorni
authors.affiliation: Department of Biology, Università di Firenze, via Micheli 1, 50121, Firenze, Italy. Electronic address: ilaria.colzi@unifi.it.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Colzi
authors.affiliation: Department of Biology, Università di Firenze, via Micheli 1, 50121, Firenze, Italy. Electronic address: sara.pignattelli@gmail.it.
authors.forename: Sara
authors.initials: S
authors.lastname: Pignattelli
authors.affiliation: Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019, Sesto Fiorentino, Italy. Electronic address: nadia.bazihizina@unifi.it.
authors.forename: Nadia
authors.initials: N
authors.lastname: Bazihizina
authors.affiliation: Department of Earth Science, Università di Firenze, via La Pira 4, 50121, Firenze, Italy. Electronic address: antonella.buccianti@unifi.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Buccianti
authors.affiliation: Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134, Firenze, Italy. Electronic address: simone.luti@unifi.it.
authors.forename: Simone
authors.initials: S
authors.lastname: Luti
authors.affiliation: Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134, Firenze, Italy. Electronic address: uigia.pazzagli@unifi.it.
authors.forename: Luigia
authors.initials: L
authors.lastname: Pazzagli
authors.affiliation: Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019, Sesto Fiorentino, Italy. Electronic address: stefano.mancuso@unifi.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mancuso
authors.affiliation: Department of Biology, Università di Firenze, via Micheli 1, 50121, Firenze, Italy. Electronic address: cristina.gonnelli@unifi.it.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gonnelli
mesh_terms: D000222:Adaptation, Physiological; D003300:Copper / Q000378:metabolism* / Q000633:toxicity; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D029751:Silene / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D055549:Volatile Organic Compounds; D008315:Malondialdehyde; D003300:Copper; C048306:callose; D006861:Hydrogen Peroxide
keywords: Biotic interactions; Callose; Heavy metals; Oxidative stress; VOCs