title: miR-493 mediated DKK1 down-regulation confers proliferation, invasion and chemo-resistance in gastric cancer cells.
abstract: In the present study, we demonstrated that the levels of DKK1 were decreased in serums and tissues of GC. DKK1 levels inversely correlated with tumor class, TNM stage, distant metastasis and lymph node metastasis of GC. GC patients with low DKK1 levels had a poor overall survival. DKK1 inhibited the proliferation of GC cells in vitro and in vivo. DKK1 also inhibited invasion, but enhanced chemo-sensitivity of GC cells. Mechanically, miR-493 levels increased in GC and directly targeted and down-regulated DKK1 expression. In agreement, miR-493 promoted proliferation of GC cells in vitro and in vivo. MiR-493 also promoted invasion and chemo-resistance of GC cells. However, DKK1 overexpression reversed the effects of miR-493 on proliferation, invasion and chemo-sensitivity. Thus, our results provide new insight for the role of miR-493/DKK1 axis in GC.
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Jia
authors.affiliation: Department of Gastrointestinal Neoplasms Surgery, Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Peng
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Yingen
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Gastrointestinal Neoplasms Surgery, Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Minying
authors.initials: M
authors.lastname: Lu
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yin
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Guopei
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Department of Gastrointestinal Neoplasms Surgery, Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Cancer Research Institute and Cancer Center of Guangzhou Medical University, Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Guangzhou 510095, Guangdong, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D015153:Blotting, Western; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D015331:Cohort Studies; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; C501126:DKK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; C555448:MIRN493 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: DKK1; gastric cancer; invasion; miR-493; proliferation