title: A novel Meloidogyne enterolobii effector MeTCTP promotes parasitism by suppressing programmed cell death in host plants.
abstract: Meloidogyne enterolobii is one of the most important plant-parasitic nematodes that can overcome the Mi-1 resistance gene and damage many economically important crops. Translationally controlled tumour protein (TCTP) is a multifunctional protein that exists in various eukaryotes and plays an important role in parasitism. In this study, a novel M. enterolobii TCTP effector, named MeTCTP, was identified and functionally characterized. MeTCTP was specifically expressed within the dorsal gland and was up-regulated during M. enterolobii parasitism. Transient expression of MeTCTP in protoplasts from tomato roots showed that MeTCTP was localized in the cytoplasm of the host cells. Transgenic Arabidopsis thaliana plants overexpressing MeTCTP were more susceptible to M. enterolobii infection than wild-type plants in a dose-dependent manner. By contrast, in planta RNA interference (RNAi) targeting MeTCTP suppressed the expression of MeTCTP in infecting nematodes and attenuated their parasitism. Furthermore, MeTCTP could suppress programmed cell death triggered by the pro-apoptotic protein BAX. These results demonstrate that MeTCTP is a novel plant-parasitic nematode effector that promotes parasitism, probably by suppressing programmed cell death in host plants.
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Zhuo
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Borong
authors.initials: B
authors.lastname: Lin
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Sun
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Hu
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Vocational College of Ecological Engineering, Guangzhou, 510520, China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Liao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D017360:Arabidopsis / Q000235:genetics / Q000469:parasitology*; D003593:Cytoplasm / Q000378:metabolism; D004198:Disease Susceptibility; D004947:Esophagus / Q000378:metabolism; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000737:chemistry / Q000378:metabolism*; D006790:Host-Parasite Interactions; D008018:Life Cycle Stages / Q000235:genetics; D018551:Solanum lycopersicum / Q000166:cytology / Q000469:parasitology*; D010271:Parasites / Q000378:metabolism*; D010935:Plant Diseases / Q000469:parasitology; D030821:Plants, Genetically Modified; D034622:RNA Interference; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000469:parasitology*; D014431:Tylenchoidea / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D051028:bcl-2-Associated X Protein
keywords: Meloidogyne enterolobii; RNAi; overexpression; programmed cell death suppression; transgenic Arabidopsis; translationally controlled tumour protein