title: Oxidative stress and altered steroidogenesis in the ovary by cholinergic stimulation of coeliac ganglion in the first proestrous in rats. Implication of nitric oxide.
abstract: An ex-vivo Coeliac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-O) system from virgin rats in the first proestrous was used to test whether cholinergic stimulation of CG affects oxidative status and steroidogenesis in the ovary. The CG and the O were placed in separate buffered-compartments, connected by the SON, and the CG was stimulated by acetylcholine (Ach). To test a possible role of nitric oxide (NO) in the ovarian response to cholinergic stimulation of CG, aminoguanidine (AG) - an inhibitor of inducible-NO synthase was added to the O compartment. After 180 min incubation, the oxidative status was assessed in O whereas nitrite and steroidogenesis were assessed at 30, 120 and 180 min. Ach in CG decreased the total antioxidant capacity, but increased NO production and protein carbonization in O. Ach stimulation of CG increased estradiol, but decreased progesterone release in O by reducing the mRNAs related to their synthesis and degradation. The addition of AG to the O compartment caused an opposite effect, which was more pronounced in the presence of Ach in the CG compartment than in its absence. These results show that the stimulation of the extrinsic-cholinergic innervation of the O increases the concentration of NO, causes oxidative stress and modulates steroidogenesis in the first rat proestrous.
authors.affiliation: Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: María B
authors.initials: MB
authors.lastname: Delsouc
authors.affiliation: Laboratorio de Medicina Experimental y Traduccional (LME&T), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: María C
authors.initials: MC
authors.lastname: Della Vedova
authors.affiliation: Laboratorio de Medicina Experimental y Traduccional (LME&T), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Darío
authors.initials: D
authors.lastname: Ramírez
authors.affiliation: Laboratorio de Cronobiología (LABCRON), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Anzulovich
authors.affiliation: Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Delgado
authors.affiliation: Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: mcasais@unsl.edu.ar.
authors.forename: Marilina
authors.initials: M
authors.lastname: Casais
mesh_terms: D000818:Animals; D018678:Cholinergic Agents / Q000494:pharmacology*; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011359:Proestrus / Q000187:drug effects; D011374:Progesterone / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018678:Cholinergic Agents; D009569:Nitric Oxide; D011374:Progesterone
keywords: Acetylcholine; Aminoguanidine; First proestrous in rats; Nitric oxide; Ovarian steroidogenesis; Oxidative stress