title: Different response of osteoblastic cells to Mg(2+), Zn(2+) and Sr(2+) doped calcium silicate coatings.
abstract: Mg(2+), Zn(2+) and Sr(2+) substitution for Ca(2+) in plasma sprayed calcium silicate (Ca-Si) coatings have been reported to impede their degradation in physiological environment and, more importantly, to improve their biological performance. The reason for the improved biological performance is still elusive and, especially, the contribution of the dopant ions is lack of obvious and direct evidence. In this study, we aim to identify the effect of Mg(2+), Zn(2+) and Sr(2+) incorporation on the osteogenic ability of Ca-Si based coatings (Ca2MgSi2O7, Ca2ZnSi2O7 and Sr-CaSiO3) by minimizing the influence of Ca and Si ions release and surface physical properties. Similar surface morphology, crystallinity and roughness were achieved for all samples by optimizing the spray parameters. As expected, Ca and Si ions release from all the coatings showed the comparable concentration with immersing time. The response of MC3T3-E1 cells onto Mg(2+), Zn(2+) and Sr(2+) doped Ca-Si coatings were studied in terms of osteoblastic adhesion, proliferation, differentiation and mineralization. The results showed that the level of cell adhesion and proliferation increased the most on the surface of Mg-modified coating. Gene expressions of early markers of osteoblast differentiation (COL-I and ALP mRNA) were obviously improved on Zn-modified coating. Gene expressions of later markers for osteoblast differentiation (OPN and OC mRNA) and mineralized nodules formation were obviously accelerated on the surface of Sr-modified coating. Since Mg(2+), Zn(2+) and Sr(2+) play a regulatory role in different stages of osteogenesis, it may be possible to utilize this in the development of new coating materials for orthopedic application.
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Hu
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China. likai@mail.sic.ac.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China.
authors.forename: Houhua
authors.initials: H
authors.lastname: Pan
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People's Republic of China. xbzheng@mail.sic.ac.cn.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001031:Apatites; D017610:Calcium Compounds / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation; D008274:Magnesium / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis; D017640:Silicates / Q000737:chemistry*; D013324:Strontium / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors; D054457:Tissue Scaffolds; D015032:Zinc / Q000737:chemistry*
chemical_list: D001031:Apatites; D017610:Calcium Compounds; D017640:Silicates; D008274:Magnesium; D015032:Zinc; C031293:calcium silicate; D013324:Strontium