title: An Unusual Stress Metabolite from a Hydrothermal Vent Fungus Aspergillus sp. WU 243 Induced by Cobalt.
abstract: A novel hybrid polyketide-terpenoid, aspergstressin (1), possessing a unique fused polycyclic structure, was induced from culture broth of strain Aspergillus sp. WU 243 by cobalt ion stimulation. The strain was isolated from the digestive gland of Xenograpsus testudinatus, a unique type of crab which dwells in the Kueishantao hydrothermal vents off Taiwan. The chemical structure and relative configuration of the stress metabolite were established by spectroscopic means. Aspergillus sp. WU 243 produced aspergstressin (1) only under cobalt stressed culture conditions. The results show that stress-driven discovery of new natural products from hydrothermal vent fungi is an effective strategy to unveil the untapped reservoir of small molecules from species found in the hydrothermal vent environment.
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, China. dingchihong@163.com.
authors.forename: Chihong
authors.initials: C
authors.lastname: Ding
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, China. wxd_zju@163.com.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wu
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, China. naz22ia@hotmail.com.
authors.forename: Bibi Nazia
authors.initials: BN
authors.lastname: Auckloo
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, China. ctchen@faculty.nsysu.edu.tw.|Institute of Marine Geology and Chemistry, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. ctchen@faculty.nsysu.edu.tw.
authors.forename: Chen-Tung Arthur
authors.initials: CT
authors.lastname: Chen
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, China. gsyeying@zju.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310058, China. wkwnpc@zjgsu.edu.cn.
authors.forename: Kuiwu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, China. wubin@zju.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D000818:Animals; D001230:Aspergillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D003386:Brachyura / Q000382:microbiology; D002413:Cations, Divalent; D003035:Cobalt / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D060086:Hydrothermal Vents; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater; D013312:Stress, Physiological; D013624:Taiwan; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D003470:Culture Media; D061065:Polyketides; D013729:Terpenes; D003035:Cobalt
keywords: hydrothermal vent; marine fungi; marine natural products; metal-stress