title: Novel Recombinant Protein FlaA N/C Protects against Radiation Injury via NF-κB Signaling.
abstract: There are few safe and effective drugs available that protect healthy tissue against radiation-induced damage, highlighting the need to develop such radioprotective agents. We investigated the mechanism underlying the protective effects of the novel, recombinant, flagellin-like protein FlaA N/C against radiation-induced tissue damage in female BALB/c mice. FlaA N/C treatment increased the levels of several pro-proliferative cytokines while inhibiting apoptosis and reducing inflammation. These effects were accompanied by activation of the nuclear factor κB signaling pathway via direct interaction of FlaA N/C with Toll-like receptor 5, as well as enhanced survival of mice after total-body irradiation compared to that observed with treatment with amifostine, a radioprotective agent that is currently being used in clinical practice. These results indicate that FlaA N/C could be further explored as a possible protector of damage to healthy tissue during radiotherapy.
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Wu
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Yuanchun
authors.initials: Y
authors.lastname: Fan
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Peigeng
authors.initials: P
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Du
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Shi
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhou
mesh_terms: D004999:Amifostine / Q000008:administration & dosage; D000818:Animals; D016207:Cytokines / Q000276:immunology; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005408:Flagellin / Q000008:administration & dosage* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000276:immunology*; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000209:etiology / Q000276:immunology* / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects / Q000276:immunology; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D015398:Signal Transduction / Q000276:immunology* / Q000528:radiation effects; D015996:Survival Rate; D016896:Treatment Outcome; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D005408:Flagellin; C067138:flaA protein, bacteria; D004999:Amifostine