title: Immobilization of cells and enzymes to LentiKats®.
abstract: Biocatalyst immobilization is one of the techniques, which can improve whole cells or enzyme applications. This method, based on the fixation of the biocatalyst into or onto various materials, may increase robustness of the biocatalyst, allows its reuse, or improves the product yield. In recent decades, a number of immobilization techniques have been developed. They can be divided according to the used natural or synthetic material and principle of biocatalyst fixation in the particle. One option, based on the entrapment of cells or enzymes into a synthetic polyvinyl alcohol lens with original shape, is LentiKats® immobilization. This review describes the preparation principle of these particles and summarizes existing successful LentiKats® immobilizations. In addition, examples are compared with other immobilization techniques or free biocatalysts, pointing to the advantages and disadvantages of LentiKats®.
authors.affiliation: Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Krasňan
authors.affiliation: LentiKat's a.s., Pod Vinicí 83, 471 27, Stráž pod Ralskem, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Stloukal
authors.affiliation: Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
authors.forename: Michal
authors.initials: M
authors.lastname: Rosenberg
authors.affiliation: Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia. martin.rebros@stuba.sk.
authors.forename: Martin
authors.initials: M
authors.lastname: Rebroš
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002448:Cell Adhesion; D018914:Cells, Immobilized / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000378:metabolism; D011485:Protein Binding
chemical_list: D004800:Enzymes, Immobilized; D011142:Polyvinyl Alcohol
keywords: Biocatalyst; Immobilization; LentiKats; Polyvinyl alcohol