title: A simple, accurate, time-saving and green method for the determination of 15 sulfonamides and metabolites in serum samples by ultra-high performance supercritical fluid chromatography.
abstract: An analytical method based on ultra-high performance supercritical fluid chromatography (UHPSFC) with photo-diode array detection (PDA) has been developed to quantify 15 sulfonamides and their N4-acetylation metabolites in serum. Under the optimized gradient elution conditions, it took only 7min to separate all 15 sulfonamides and the critical pairs of each parent drug and metabolite were completely separated. Variables affecting the UHPSFC were optimized to get a better separation. The performance of the developed method was evaluated. The UHPSFC method allowed the baseline separation and determination of 15 sulfonamides and metabolites with limit of detection ranging from 0.15 to 0.35μg/mL. Recoveries between 90.1 and 102.2% were obtained with satisfactory precision since relative standard deviations were always below 3%. The proposed method is simple, accurate, time-saving and green, it is applicable to a variety of sulfonamides detection in serum samples.
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; School of Pharmacy, China Medical University, Shenyang 110013, China. Electronic address: 13840149878@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Wei-E
authors.initials: WE
authors.lastname: Zhou
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Li
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Ren
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Li
authors.affiliation: School of Pharmacy, China Medical University, Shenyang 110013, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, China Medical University, Shenyang 110013, China.
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Feng
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China. Electronic address: fengzhang@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000379:methods; D006801:Humans; D013424:Sulfanilamides / Q000097:blood; D013449:Sulfonamides / Q000097:blood*
chemical_list: D013424:Sulfanilamides; D013449:Sulfonamides
keywords: PDA; Resolution; Serum; Sulfonamides; Ultra-high performance supercritical fluid chromatography (UHPSFC)