title: Affinity purification of egg yolk immunoglobulins (IgY) using a human mycoplasma protein.
abstract: Egg yolk immunoglobulin (IgY) is a superior functional equivalent to mammalian IgG. However, the preparation of refined and highly purified IgY is still attributed as difficult task. Protein M (a transmembrane protein from human mycoplasma) has been newly demonstrated as an ideal affinity regent for mammalian antibody purification. This study aimed to evaluate the interaction between protein M and IgY. The results showed protein M could be a superior affinity reagent for IgY, scFv as well as IgYΔFc, based on pull down and western blot investigations; in addition, it was found that ∼125 times increase of effective IgY in the elutent was obtained using protein M affinity chromatography column compared with traditional IgY extraction methods. This indicates, the purification strategy of protein M is entirely different to traditional IBPs and the salient purification feature of protein M would be a breakthrough for purifying not only non-mammalian antibodies, but also monoclonal antibodies and engineered antibodies based on variable region.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Thirumalai
authors.initials: T
authors.lastname: Diraviyam
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. Electronic address: zhang.xy@nwsuaf.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D004530:Egg Yolk / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D045704:Mycoplasma genitalium
chemical_list: D001426:Bacterial Proteins; C016512:IgY; D007136:Immunoglobulins
keywords: Affinity purification; Egg yolk immunoglobulin (IgY); Protein M