title: High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest.
abstract: Extracts of plants from the Malaysian rainforest and other fragile habitats are being researched intensively for identification of beneficial biological actions, with assessment of antioxidant behavior being a common component of such assessments. A number of tests for antioxidant behavior are used, with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reduction activity potential (FRAP) assays often being used in parallel, and also with measurement of total phenolics content (TPC) as a surrogate marker for antioxidant capacity. The present study investigated the possible redundancy in using all three assays to determine antioxidant capacity in 92 extracts obtained from 27 plants from the Malaysian rainforest. The results demonstrated that the assays displayed a high (R ≥ 0.82) and significant (P < 0.0001) correlation with one another, indicating a high level of redundancy if all three assays are used in parallel. This appears to be a waste of potentially valuable plant extracts. Because of problems with the FRAP assay relating to color interference and variable rates of reaction point, the DPPH assay is the preferred assay in preliminary screening of extracts of plants from the Malaysian rainforest.
authors.affiliation: School of Biomedical Sciences, University of Nottingham, Nottingham NG7 2UH, UK. garry.clarke@nottingham.ac.uk.
authors.forename: Garry
authors.initials: G
authors.lastname: Clarke
authors.affiliation: School of Biomedical Sciences, University of Nottingham, Jalan Broga, 43500, Semenyih, Selangor, Malaysia. Kang-Nee.Ting@nottingham.edu.my.
authors.forename: Kang Nee
authors.initials: KN
authors.lastname: Ting
authors.affiliation: School of Biomedical Sciences, University of Nottingham, Jalan Broga, 43500, Semenyih, Selangor, Malaysia. Christophe.Wiart@nottingham.edu.my.
authors.forename: Christophe
authors.initials: C
authors.lastname: Wiart
authors.affiliation: School of Biomedical Sciences, University of Nottingham, Nottingham NG7 2UH, UK. jeff.fry@nottingham.ac.uk.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Fry
keywords: DPPH; FRAP; antioxidant; correlation; phenolics; plant extracts