title: Xuhuaishuia manganoxidans gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province.
abstract: A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, manganese-oxidizing bacterial strain, designated DY6-4T, was isolated from the surface sediment of the Pacific Clarion-Clipperton Fracture Zone. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain DY6-4T formed a lineage within the family Rhodobacteraceae and was distinct from the most closely related genera Sulfitobacter, Aliiroseovarius and Loktanella (94.0-96.0 %, 93.4-96.0 % and 91.9-95.9 % 16S rRNA gene sequence similarity, repectively). Optimal growth occurred in the presence of 1 % (w/v) NaCl, at pH 7.0 and at 28 °C. Strain DY6-4T contained ubiquinone-10 (Q-10) as the major ubiquinone, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and one unidentified aminolipid as the predominant polar lipids, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the main fatty acids (>10 % of the total). The DNA G+C content of strain DY6-4T was 66.6 mol%. On the basis of the polyphasic analyses, strain DY6-4T is considered to represent a novel species of a novel genus in the Roseobacter clade of the family Rhodobacteraceae, for which the name Xuhuaishuia manganoxidans gen. nov., sp. nov. is proposed. The type strain is DY6-4T ( = KCTC 42421T = MCCC 1K00502T).
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiaochong
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Dai
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008345:Manganese / Q000378:metabolism*; D010140:Pacific Ocean; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D008345:Manganese; C025204:Ubiquinone Q2