title: [Diversity of uncultured actinomycetes in saline-alkali soil from Jiuquan area of Hexi Corridor].
abstract: In order to more accurately understand community structure and diversity of actinomycetes in saline-alkali soil from Jiuquan area of Hexi Corridor, the community structure and diversity from three kinds of soil samples (primary, secondary saline alkali soil and farmland soil) were analyzed using uncultured methods. The results showed that the 16S rDNA clone library of actinomycetales from the primary saline-alkali soil belonged to 19 OTUs, Micrococcineae, Propionibacterineae, Corynebacterineae, Frankineae, Pseudonocardineae and unknown groups of Actinomycetales; the 16S r DNA clone library of actinomycetales from the secondary saline-alkali soil belonged to 14 OTUs, Micrococcineae, Propionibacterineae, Corynebacterineae, Frankineae, Pseudonocardineae and unknown groups of Actinomycetales; the 16S rDNA clone library of farmland soil belonged to 7 OTUs, Micrococcineae, Propionibacterineae, Corynebacterineae, Frankineae, Pseudonocardineae and unknown groups of Actinomycetales; Micrococcineae was the common population in the three soils, and also was the dominant population in primary saline alkali soil and farmland soil. The diversity index and rarefaction curves analysis showed that actinomycetes species richness was in order of primary saline-alkali soil > secondary saline-alkali soil > farmland soil. The dilution curves of primary saline-alkali soil and secondary saline-alkali soil were not leveled off, which indicated the actinomycetes diversity in saline-alkali soil was more enriched than the actual. The rich and diverse actinomycetes resources in saline-alkali soil from Jiuquan area of Hexi Corridor provide important data on the actinomycetes ecology distribution research, exploitation and utilization in saline-alkali soil.
authors.forename: Hai-yun
authors.initials: HY
authors.lastname: Li
authors.forename: Shi-quan
authors.initials: SQ
authors.lastname: Niu
authors.forename: Wei-bao
authors.initials: WB
authors.lastname: Kong
authors.forename: Wei-ru
authors.initials: WR
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Geng
authors.forename: Cai-hong
authors.initials: CH
authors.lastname: Han
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Da
authors.forename: Ai-mei
authors.initials: AM
authors.lastname: Zhang
authors.forename: Xue-tai
authors.initials: XT
authors.lastname: Zhu
mesh_terms: D039903:Actinobacteria / Q000145:classification*; D000468:Alkalies; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D000468:Alkalies; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil