title: Quantitative Appraisal of the Probiotic Attributes and In Vitro Adhesion Potential of Anti-listerial Bacteriocin-producing Lactic Acid Bacteria.
abstract: Estimation of bile tolerance, endurance to gastric and intestinal environment and adhesion potential to intestinal cells are significant selection criteria for probiotic lactic acid bacteria (LAB). In this paper, the probiotic potential of native bacteriocin-producing LAB isolated previously from indigenous source has been determined through quantitative approaches. Among fifteen anti-listerial bacteriocin-producing native LAB, ten strains were found to be bile tolerant. The presence of bile salt hydrolase (bsh) gene in native Lactobacillus plantarum strains was detected by PCR and confirmed by nucleic acid sequencing of a representative amplicon. Interestingly, three native LAB strains exhibited significant viability in simulated gastric fluid, analogous to the standard LAB Lactobacillus rhamnosus GG, while an overwhelming majority of the native LAB strains demonstrated the ability to survive and remain viable in simulated intestinal fluid. Quantitative adhesion assays based on conventional plating method and a fluorescence-based method revealed that the LAB isolates obtained from dried fish displayed significant in vitro adhesion potential to human adenocarcinoma HT-29 cells, and the adhesion level was comparable to some of the standard probiotic LAB strains. The present study unravels putative probiotic attributes in certain bacteriocin-producing LAB strains of non-human origin, which on further in vivo characterization could find specific applications in probiotic food formulations targeted for health benefits.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, Assam, India.
authors.forename: Sandipan
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, Assam, India.
authors.forename: Atul Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, Assam, India.
authors.forename: Manab Deb
authors.initials: MD
authors.lastname: Adhikari
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, Assam, India. aramesh@iitg.ernet.in.
authors.forename: Aiyagari
authors.initials: A
authors.lastname: Ramesh
keywords: Adhesion; Bacteriocin; Lactic acid bacteria; Probiotic