title: Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest.
abstract: A novel Streptomyces strain, MUSC 119(T), was isolated from a soil collected from a mangrove forest. Cells of MUSC 119(T) stained Gram-positive and formed light brownish grey aerial mycelium and grayish yellowish brown substrate mycelium on ISP 2 medium. A polyphasic approach was used to determine the taxonomic status of strain MUSC 119(T), which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces. The cell wall peptidoglycan consisted of LL-diaminopimelic acid. The predominant menaquinones were identified as MK-9(H8), MK-9(H6) and MK-9(H4). The polar lipid profile consisted of phosphatidylinositol, phosphatidylethanolamine, glycolipids, diphosphatidylglycerol and four phospholipids. The predominant cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The cell wall sugars were glucose, mannose, ribose and rhamnose. The phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain MUSC119(T) to be closely related to Streptomyces rhizophilus JR-41(T) (99.0 % sequence similarity), S. panaciradicis 1MR-8(T) (98.9 %), S. gramineus JR-43(T) (98.8 %) and S. graminisoli JR-19(T) (98.7 %). These results suggest that MUSC 119(T) should be placed within the genus Streptomyces. DNA-DNA relatedness values between MUSC 119(T) to closely related strains ranged from 14.5 ± 1.3 to 27.5 ± 0.7 %. The G+C content was determined to be 72.6 mol %. The polyphasic study of MUSC 119(T) showed that this strain represents a novel species, for which the name Streptomyces humi sp. nov. is proposed. The type strain of S. humi is MUSC 119(T) (=DSM 42174(T) = MCCC 1K00505(T)).
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 46150, Bandar Sunway, Selangor Darul Ehsan, Malaysia.|Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Nurullhudda
authors.initials: N
authors.lastname: Zainal
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 46150, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
authors.forename: Hooi-Leng
authors.initials: HL
authors.lastname: Ser
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Department of Medicine, Faculty of Medicine, Centre of Excellent for Research in AIDS (CERiA), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Kok-Keng
authors.initials: KK
authors.lastname: Tee
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 46150, Bandar Sunway, Selangor Darul Ehsan, Malaysia. lee.learn.han@monash.edu.
authors.forename: Learn-Han
authors.initials: LH
authors.lastname: Lee
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D065928:Forests; D016680:Genome, Bacterial; D023281:Genomics; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Actinobacteria; Malaysia; Mangrove forest; Streptomyces humi sp. nov