title: Prototyping the Experimental Setup to Quantify the Tissue Oxygen Consumption Rate and Its Preliminary Test.
abstract: In order to establish a reliable and practical method to make a diagnosis on the viability of an amputated extremity, we propose a method to evaluate the oxygen consumption rate. To validate this concept, we prototyped an experimental system with which the oxygen transfer rate into tissue can be assessed by the rate of change of the decrease in dissolved oxygen (DO) concentration within the buffer fluid surrounding the target tissue. The purpose of this study is to examine the feasibility of our prototyped experimental system by comparison between fresh and non-fresh rat skeletal muscles. The results show that the fresher tissue transferred more oxygen to the tissue, which suggests that tissue oxygen consumption is highly related to tissue freshness and can indirectly assess the tissue viability.
authors.affiliation: Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-Ku, Saitama, 337-8570, Japan. nobuo@sic.shibaura-it.ac.jp.|Systems Engineering and Science, Graduate school of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan. nobuo@sic.shibaura-it.ac.jp.
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.affiliation: Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-Ku, Saitama, 337-8570, Japan.|Systems Engineering and Science, Graduate school of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shibata
authors.affiliation: Systems Engineering and Science, Graduate school of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sawada
authors.affiliation: Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-Ku, Saitama, 337-8570, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mizukami
mesh_terms: D000818:Animals; D010101:Oxygen Consumption; D051381:Rats
keywords: Dissolved oxygen; Oxygen diffusion; Temperature; Tissue freshness; Tissue viability