title: Syzyguim guineense Extracts Show Antioxidant Activities and Beneficial Activities on Oxidative Stress Induced by Ferric Chloride in the Liver Homogenate.
abstract: The aim of this study was to determine the in vitro antioxidant activity, free radical scavenging property and the beneficial effects of extracts of various parts of Syzygium guineense in reducing oxidative stress damage in the liver. The effects of extracts on free radicals were determined on radicals DPPH, ABTS, NO and OH followed by the antioxidant properties using Ferric Reducing Antioxidant Power assay (FRAP) and hosphomolybdenum (PPMB). The phytochemical screening of these extracts was performed by determination of the phenolic content. The oxidative damage inhibition in the liver was determined by measuring malondialdehyde (MDA) as well as the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase. Overall, the bark extract of the ethanol/water or methanol showed the highest radical scavenging activities against DPPH, ABTS and OH radicals compared to the other extracts. This extract also contained the highest phenolic content implying the potential contribution of phenolic compounds towards the antioxidant activities. However, the methanol extract of the root demonstrated the highest protective effects of SOD and CAT against ferric chloride while the hydro-ethanol extract of the leaves exhibited the highest inhibitory effects on lipid peroxidation. These findings suggest that antioxidant properties of S. guineense extracts could be attributed to phenolic compounds revealed by phytochemical studies. Thus, the present results indicate clearly that the extracts of S. guineense possess antioxidant properties and could serve as free radical inhibitors or scavengers, acting possibly as primary antioxidants. The antioxidant properties of the bark extract may thus sustain its various biological activities.
authors.affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. apieme@yahoo.fr.
authors.forename: Constant Anatole
authors.initials: CA
authors.lastname: Pieme
authors.affiliation: Department of pharmacy, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. jngoupayo@yahoo.com.
authors.forename: Joseph
authors.initials: J
authors.lastname: Ngoupayo
authors.affiliation: Department of pharmacy, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. khoukouzherve@yahoo.fr.
authors.forename: Claude Herve Khou-Kouz
authors.initials: CH
authors.lastname: Nkoulou
authors.affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. moukette2006@yahoo.fr.
authors.forename: Bruno Moukette
authors.initials: BM
authors.lastname: Moukette
authors.affiliation: Department of Biochemistry, Faculty of Sciences University of Yaounde I, P.O. Box 812 Yaounde, Cameroon. njinoborle@yahoo.fr.
authors.forename: Borgia Legrand Njinkio
authors.initials: BL
authors.lastname: Nono
authors.affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. movicky@yahoo.fr.
authors.forename: Vicky Jocelyne Ama
authors.initials: VJ
authors.lastname: Moor
authors.affiliation: Department of Anesthesia and Reanimation, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. minkandeze@yahoo.fr.
authors.forename: Jacqueline Ze
authors.initials: JZ
authors.lastname: Minkande
authors.affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364 Yaounde, Cameroon. jngogang@yahoo.fr.
authors.forename: Jeanne Yonkeu
authors.initials: JY
authors.lastname: Ngogang
keywords: Syzygium guineense; antioxidant activity; lipid peroxidation; phenolic content; radical scavenging activity