title: A fluorescence enhancement-based label-free homogeneous immunoassay of benzo[a]pyrene (BaP) in aqueous solutions.
abstract: A fluorescence enhancement-based immunoassay has been developed for the detection of the polycyclic aromatic hydrocarbons (PAH), benzo[a]pyrene (BaP), in aqueous solutions. The results of this study show that BaP, which inefficiently fluoresces in aqueous solution, displays enhanced fluorescence when bound to the anti-BaP antibody (anti-BaP), as part of a label-free immunoassay system. Binding to anti-BaP results in a 3.12-fold increase in the fluorescence intensity of BaP, which emits at 435 nm when excited at 280 nm, due to the hydrophobic interaction and fluorescence resonance energy transfer (FRET) between antibody and antigen. As result of this phenomenon, the antibody-based fluorescence immunoassay system can be used to detect BaP specifically with a limit of detection (LOD) of 0.06 ng mL(-1). Finally, extraction recoveries of BaP from spiked wheat and barley samples were found to be in the range of 80.5-87.0% and 92.9-92.1%, respectively.
authors.affiliation: College of Biology and the Environment, Nanjing Forestry University, Nanjing 210-037, China.
authors.forename: Taihua
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science & Technology (GIST), 261 Cheomdan-gwagiro, Gwangju 500-712, South Korea; Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
authors.forename: Yo Han
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Biomedical Translational Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, South Korea.
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Shin
authors.affiliation: Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science & Technology (GIST), 261 Cheomdan-gwagiro, Gwangju 500-712, South Korea; Advanced Photonics Research Institute, Gwangju Institute of Science & Technology (GIST), 261 Cheomdan-gwagiro, Gwangju 500-712, South Korea. Electronic address: mkim@gist.ac.kr.
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D001564:Benzo(a)pyrene / Q000032:analysis*; D004785:Environmental Pollutants / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D015200:Fluoroimmunoassay / Q000379:methods*; D001467:Hordeum / Q000737:chemistry; D057230:Limit of Detection; D012996:Solutions; D014908:Triticum / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D004785:Environmental Pollutants; D012996:Solutions; D001564:Benzo(a)pyrene
keywords: Benzo[a]pyrene; FRET; Fluorescence enhancement; Fluorescence immunoassay; Polycyclic aromatic hydrocarbons