title: High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry.
abstract: In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve about 125,880 cells per minute (more than one hundred and twenty five thousand cells per minute), even while employing cost-effective low frame rate cameras (120 fps). The throughput achieved here is a notable progression in the field of diagnostics as it enables rapid quantitative testing and analysis. We demonstrate the applicability of the instrument to point-of-care diagnostics, by performing blood cell counting. We report a comparative analysis between the counts (in cells per μl) obtained from our instrument, with that of a commercially available hematology analyzer.
authors.affiliation: Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied Physics, Indian Institute of Science (IISc), Bangalore, India, 560012.
authors.forename: Veerendra Kalyan
authors.initials: VK
authors.lastname: Jagannadh
authors.affiliation: Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012.
authors.forename: Bindu Prabhath
authors.initials: BP
authors.lastname: Bhat
authors.affiliation: Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012.
authors.forename: Lourdes Albina
authors.initials: LA
authors.lastname: Nirupa Julius
authors.affiliation: Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied Physics, Indian Institute of Science (IISc), Bangalore, India, 560012. saisiva.gorthi@iap.iisc.ernet.in.|Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012. saisiva.gorthi@iap.iisc.ernet.in.
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi
mesh_terms: D004867:Equipment Design; D004906:Erythrocyte Count / Q000191:economics / Q000295:instrumentation*; D005434:Flow Cytometry / Q000191:economics / Q000295:instrumentation*; D057166:High-Throughput Screening Assays / Q000191:economics / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D008853:Microscopy / Q000191:economics / Q000295:instrumentation*
keywords: Clinical / biomedical analysis; High throughput screening; Microfluidic biosensors