title: Structural elucidation and protective role of a polysaccharide from Sargassum fusiforme on ameliorating learning and memory deficiencies in mice.
abstract: A fucoidan, Sargassum fusiforme polysaccharide 65 (SFPS65) A, was isolated from a brown alga (S. fusiforme). SFPS65A had an estimated molecular weight of 90kDa and showed αD(20) -74.3288 (c 0.05, H2O). SFPS65A is composed of fucose, galactose, xylose, glucose, glucuronic acid, and mannose in the ratio of 19.23:9.58:6.64:1:6.52:2.57. The structural features of SFPS65A were investigated using composition analysis, methylation analysis, infrared spectrum, nuclear magnetic resonance spectroscopy, and electrospray ionization quadruple time-of-flight tandem mass spectroscopy. Results showed that SFPS65A has a main chain composed of →3)-β-l-Fucp-(1→3,4)-β-l-Fucp-(1→3,4)-β-l-Fucp-(1→ and connected with →3,4)-α-d-GlcAp-(1→, →4)-β-d-Xylp-(1→, →4)-α-d-Galp-(1→, →3,6)-α-d-Manp-(1→ alternately. The branches at O-3 of the fucosyl residue and O-3 of the hexosyl residues may include sulfate, →4)-β-l-Fucp-(1→, β-d-Xylp-(1→, and β-d-Xylp-(1→. SFPS65A exhibited an activity on Alzheimer's disease in vivo in the pharmacological experiments by increasing the cognitive abilities of scopolamine-, ethanol-, and sodium nitrite-treated mice against memory deficits.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, People's Republic of China; Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Hu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, People's Republic of China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, People's Republic of China.
authors.forename: Mingcang
authors.initials: M
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, People's Republic of China.
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.affiliation: Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China. Electronic address: jianjiangsg@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, People's Republic of China. Electronic address: cghsimm@126.com.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D003072:Cognition Disorders / Q000188:drug therapy*; D007858:Learning / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D008745:Methylation; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D018696:Neuroprotective Agents / Q000737:chemistry / Q000627:therapeutic use; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use; D044647:Sargassum
chemical_list: D009005:Monosaccharides; D018696:Neuroprotective Agents; D011134:Polysaccharides; C007789:fucoidan
keywords: Alzheimer's disease; Chemical structure; ESI-Q-TOF-MS; Fucoidan; Sargassum fusiforme