title: Root-shoot interactions explain the reduction of leaf mineral content in Arabidopsis plants grown under elevated [CO2 ] conditions.
abstract: Although shoot N depletion in plants exposed to elevated [CO2 ] has already been reported on several occasions, some uncertainty remains about the mechanisms involved. This study illustrates (1) the importance of characterizing root-shoot interactions and (2) the physiological, biochemical and gene expression mechanisms adopted by nitrate-fed Arabidopsis thaliana plants grown under elevated [CO2 ]. Elevated [CO2 ] increases biomass and photosynthetic rates; nevertheless, the decline in total soluble protein, Rubisco and leaf N concentrations revealed a general decrease in leaf N availability. A transcriptomic approach (conducted at the root and shoot level) revealed that exposure to 800 ppm [CO2 ] induced the expression of genes involved in the transport of nitrate and mineral elements. Leaf N and mineral status revealed that N assimilation into proteins was constrained under elevated [CO2 ]. Moreover, this study also highlights how elevated [CO2 ] induced the reorganization of nitrate assimilation between tissues; root nitrogen assimilation was favored over leaf assimilation to offset the decline in nitrogen metabolism in the leaves of plants exposed to elevated [CO2 ].
authors.affiliation: Dpto. Ciencias del Medio Natural, Universidad Pública de Navarra, Campus de Arrosadía, E-31192, Mutilva Baja, Spain.|Instituto de Agrobiotecnología (IdAB), Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, E-31192, Mutilva Baja, Spain.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jauregui
authors.affiliation: Dpto. Ciencias del Medio Natural, Universidad Pública de Navarra, Campus de Arrosadía, E-31192, Mutilva Baja, Spain.|Instituto de Agrobiotecnología (IdAB), Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, E-31192, Mutilva Baja, Spain.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Aparicio-Tejo
authors.affiliation: Biología Molecular y Bioquímica, Instituto Andaluz de Biotencología, Unidad Asociada UMA-CSIC, Universidad de Málaga, Campus Universitairo de Teatinos, E-29071, Málaga, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Avila
authors.affiliation: Biología Molecular y Bioquímica, Instituto Andaluz de Biotencología, Unidad Asociada UMA-CSIC, Universidad de Málaga, Campus Universitairo de Teatinos, E-29071, Málaga, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Cañas
authors.affiliation: Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, LT-54333, Kaunas, Lithuania.
authors.forename: Sandra
authors.initials: S
authors.lastname: Sakalauskiene
authors.affiliation: Instituto de Agrobiotecnología (IdAB), Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, E-31192, Mutilva Baja, Spain.|Dpto. Biología Vegetal y Ecología, Universidad del País Vasco, Barrio Sarriena, s/n, E-48940, Bizkaia, Spain.
authors.forename: Iker
authors.initials: I
authors.lastname: Aranjuelo
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology*; D008903:Minerals / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism
chemical_list: D008903:Minerals; D009566:Nitrates; D002245:Carbon Dioxide; D012273:Ribulose-Bisphosphate Carboxylase; D009584:Nitrogen