title: Associations of Promoter Methylations and mRNA Expressions of MMP-2, MMP-7 and MMP-9 with Primary Fallopian Tube Carcinoma.
abstract: OBJECTIVE
  To explore the associations of matrix metalloprotease-2 (MMP-2), MMP-7 and MMP-9 methylations and messenger ribonucleic acid (mRNA) expressions with primary fallopian tube carcinoma (PFTC) development and prognosis.
  
  
  METHODS
  We recruited 48 patients with PFTC into the case group and 48 healthy individuals into the control group; PFTC tissues and normal fallopian tube tissues were obtained from subjects in both groups. Methylation specific polymerase chain reaction (PCR), reverse transcription PCR and the immunohistochemical method were used to examine methylation, mRNA expressions and protein expressions of MMP-2, MMP-7 and MMP-9, respectively.
  
  
  RESULTS
  The methylation rates of MMP-2, MMP-7 and MMP-9 in the case group were significantly lower than those in the control group (all p < 0.05); MMP-2, MMP-7 and MMP-9 protein and mRNA expressions of PFTC tissues were enormously higher than those of normal tissues (all p < 0.05); univariate survival analysis indicated that MMP-2 and MMP-9 methylations and their protein expressions were associated with PFTC prognosis (all p < 0.05), which was further confirmed by the Cox regression model (all p < 0.05).
  
  
  CONCLUSIONS
  The protein and mRNA expressions of MMP-2, MMP-7 and MMP-9 might be related to PFTC, while the methylations and protein expressions of MMP-2 and MMP-9 might be associated with PFTC progression and prognosis.
authors.affiliation: Department of Gynecology, Huzhou Maternity and Child Care Hospital, Huzhou, PR China.
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Ren
authors.forename: Yinjian
authors.initials: Y
authors.lastname: Zhou
authors.forename: Mingsong
authors.initials: M
authors.lastname: Liu
authors.forename: Su
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D019175:DNA Methylation / Q000235:genetics*; D005185:Fallopian Tube Neoplasms / Q000201:enzymology / Q000235:genetics*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000235:genetics*; D020783:Matrix Metalloproteinase 7 / Q000032:analysis / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9