title: Correlation Between the Expression of MicroRNA-301a-3p and the Proportion of Th17 Cells in Patients with Rheumatoid Arthritis.
abstract: Rheumatoid arthritis (RA) is characterized by chronic synovial inflammation and subsequent joint destruction. Previous studies have confirmed that Th17 cells play a critical role in the pathogenesis of RA. MicroRNA (miR)-301a-3p is a regulatory factor for Th17 cells differentiation that contributes to the pathogenesis of autoimmune diseases. The purposes of this study were to identify the alteration of Th17 cells and analyze the correlation between the expression of the miR-301a-3p and the proportion of Th17 cells in RA patients. The results showed that the frequency of Th17 cells and the expression of transcription factors (RORγt and STAT3) significantly increased in the peripheral blood mononuclear cells (PBMCs) from RA patients, and the associated proinflammatory cytokines were also upregulated. We also observed that the expression of protein inhibitor of activated STAT3 (PIAS3), the main cellular inhibitor of STAT3, was attenuated in RA patients and negatively correlated with the percentage of Th17 cells in RA. Interestingly, miR-301a-3p, an inhibitor of PIAS3 expression, was overexpressed in the PBMCs from RA patients and positively correlated with the frequency of Th17 cells in patients with RA. Taken together, these data indicated that miR-301a-3p and Th17 cells were augmented in peripheral blood, which may play an important role in the process of RA.
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.|Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tang
authors.affiliation: The Affiliated Hospital, Jiangsu University, Zhenjiang, 212001, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yin
authors.affiliation: Department of Laboratory Medicine, Suzhou Municipal Hospital (Eastern), Suzhou, 215001, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China. tjj850913@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.|Department of Laboratory Medicine, Suzhou Hospital Traditional Chinese Medicine, Suzhou, 215009, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.|Suzhou Health College, Suzhou, 215009, China.
authors.forename: Lixian
authors.initials: L
authors.lastname: Yi
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Rui
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China. sjwjs@ujs.edu.cn.|Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China. sjwjs@ujs.edu.cn.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000473:pathology*; D018791:CD4 Lymphocyte Count; D002454:Cell Differentiation / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D018832:Molecular Chaperones / Q000096:biosynthesis*; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000096:biosynthesis; D050828:Protein Inhibitors of Activated STAT / Q000096:biosynthesis*; D050796:STAT3 Transcription Factor / Q000096:biosynthesis; D013583:Synovial Membrane / Q000473:pathology*; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; C550735:MIRN301A microRNA, human; D035683:MicroRNAs; D018832:Molecular Chaperones; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C484745:PIAS3 protein, human; D050828:Protein Inhibitors of Activated STAT; C539900:RORC protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human
keywords: Th17 cells; microrna-301a; rheumatoid arthritis