title: Characterization and antioxidant activity of a novel polysaccharide from Pholidota chinensis Lindl.
abstract: A novel polysaccharide (PP) was isolated and purified from Pholidota chinensis Lindl. The structure of PP was investigated by HPLC, IR, NMR, etc. and the antioxidant activity was evaluated by hydroxyl radical and superoxide anion assay. Results showed Pholidota chinensis Lindl. contained 3.35% (dry weight) of polysaccharides. The salt-out assay was better than sevag assay for removing protein from crude polysaccharides. The intrinsic viscosity of PP was 1.55dL/g and its average molecular weight was 45,200Da. The monosaccharide composition analysis showed that PP consisted of mannose only. Spectroscopy analysis revealed that PP was mainly composed of α-1,3 and α-1,4 linked d-mannose units with some 2-O- and 3-O-acetyl groups. Moreover, PP can scavenge superoxide anion and hydroxyl radical in a dose-dependent manner, suggesting that PP may be a potential nature antioxidant and may contribute to the analgesic activity of Pholidota chinensis Lindl.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: haihuayang2013@gmail.com.
authors.forename: Haihua
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address: wyj79821@163.com.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: 13110775827@163.com.
authors.forename: Chunji
authors.initials: C
authors.lastname: Gan
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address: yuetl@nwsuaf.edu.cn.
authors.forename: Tianli
authors.initials: T
authors.lastname: Yue
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address: yuan324@msn.com.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D017665:Hydroxyl Radical / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D029595:Orchidaceae / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity
chemical_list: D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides; D017665:Hydroxyl Radical
keywords: Antioxidant activity; Pholidota chinensis Lindl.; Polysaccharide; Structure