title: Global profiling of the signaling network of papillary thyroid carcinoma.
abstract: OBJECTIVE
  Thyroid carcinoma is one of the most fast rising cancer diagnoses in the US. Papillary thyroid carcinoma (PTC) comprises 80% of thyroid carcinoma. The goal of our study is to identify regulatory proteins and signaling pathways altered in PTC.
  
  
  METHODS
  Protein Pathway Array (PPA) was applied to screen 65 signaling proteins and phosphoproteins in 27 pairs of PTC and surrounding benign tissues. Ingenuity Pathway Analysis (IPA) was applied to analyze the signaling pathway.
  
  
  RESULTS
  11 were differentially expressed between tumors and surrounding tissues, 8 of which were up-regulated (cytokeratin 18, Stat 1, HMG-1, p-p70 S6 kinase, Raf-B, glutamine synthetase, p-PKC δ, and HDAC1), while 3 of which were down-regulated (cytokeratin 5, BECN1, and p-ERK). Further study showed that two proteins (p-p70 S6 kinase and cytokeratin 18) were associated with lymph node metastasis. The top 10 canonical pathways in PTC were identified to be involved in PTC.
  
  
  CONCLUSIONS
  Taken together, there is a broad array of dysregulation of signaling proteins in PTC, suggesting a heterogeneous group of diseases.
authors.affiliation: Departments of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
authors.forename: Kaijun
authors.initials: K
authors.lastname: Huang
authors.affiliation: Departments of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
authors.forename: Miao
authors.initials: M
authors.lastname: Cui
authors.affiliation: Departments of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.affiliation: The First Affiliated Hospital, Sun-Yat Sen University, Guangzhou 510080, China. Electronic address: easd04lyb@126.com.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Departments of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address: david.zhang@mssm.edu.
authors.forename: David
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000473:pathology*; D002291:Carcinoma, Papillary; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis; D015398:Signal Transduction; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; D010750:Phosphoproteins
keywords: Papillary thyroid carcinoma; Protein Pathway Array; Signaling pathway