title: miR-873 induces lung adenocarcinoma cell proliferation and migration by targeting SRCIN1.
abstract: microRNAs (miRNAs) are endogenously expressed, conserved and small noncoding RNA that regulate gene expression by the post-transcriptional level. In this study, we aim to examine the role of miR-873 in lung adenocarcinoma. We found that the expression of miR-873 was upregulated in four lung adenocarcinoma cell lines and tissues. In addition, the expression levels of SRCIN1 were inversely correlated with the expression levels of miR-873 in lung adenocarcinoma tissues. Furthermore, SRCIN1 was confirmed asthe direct target of miR-873 by luciferase reporter assay and Western blotting. Overexpression of miR-873 promoted the proliferation and migration of lung adenocarcinoma cells, while SRCIN1 upregulation inhibited their proliferation and migration. Restoration of SRCIN1 could significantly reverse the proliferation and migration promotion imposed by miR-873. In summary, this study reveals for the first time that miR-873 increase the lung adenocarcinoma cell proliferation and migration through directly inhibiting SRCIN1 expression.
authors.affiliation: Department of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100021, China.
authors.forename: Yushun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100021, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Department of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100021, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100021, China.
authors.forename: Minjun
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100021, China.
authors.forename: Yanjiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100021, China.
authors.forename: Shugeng
authors.initials: S
authors.lastname: Gao
keywords: Lung adenocarcinoma; SRCIN1; miR-873; microRNA