title: Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells.
abstract: One major obstacle to the application of stem cell-derived cardiomyocytes (CMs) for disease modeling and clinical therapies is the inability to identify the developmental stage of these cells without the need for genetic manipulation or utilization of exogenous markers. In this study, we demonstrate that Raman microspectroscopy can non-invasively identify embryonic stem cell (ESC)-derived chamber-specific CMs and monitor cell maturation. Using this marker-free approach, Raman peaks were identified for atrial and ventricular CMs, ESCs were successfully discriminated from their cardiac derivatives, a distinct phenotypic spectrum for ESC-derived CMs was confirmed, and unique spectral differences between fetal versus adult CMs were detected. The real-time identification and characterization of CMs, their progenitors, and subpopulations by Raman microspectroscopy strongly correlated to the phenotypical features of these cells. Due to its high molecular resolution, Raman microspectroscopy offers distinct analytical characterization for differentiating cardiovascular cell populations.
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany; Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Brauchle
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany; Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany; Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, 675 Charles E. Young Drive South, MRL 3645, Los Angeles, CA 90095, USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Knopf
authors.affiliation: Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany.
authors.forename: Hannah
authors.initials: H
authors.lastname: Bauer
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany; Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany.
authors.forename: Nian
authors.initials: N
authors.lastname: Shen
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Linder
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany; Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Monaghan
authors.affiliation: Institute of Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Kornelia
authors.initials: K
authors.lastname: Ellwanger
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany; Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany.
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Layland
authors.affiliation: Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany.
authors.forename: Sara Y
authors.initials: SY
authors.lastname: Brucker
authors.affiliation: Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, 675 Charles E. Young Drive South, MRL 3645, Los Angeles, CA 90095, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Nsair
authors.affiliation: Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569 Stuttgart, Germany; Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstrasse 7/1, 72076 Tübingen, Germany; Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, 675 Charles E. Young Drive South, MRL 3645, Los Angeles, CA 90095, USA. Electronic address: katja.schenke-layland@med.uni-tuebingen.de.
authors.forename: Katja
authors.initials: K
authors.lastname: Schenke-Layland
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D053595:Embryonic Stem Cells / Q000166:cytology; D005333:Fetus / Q000166:cytology; D006325:Heart Atria / Q000166:cytology* / Q000196:embryology; D006352:Heart Ventricles / Q000166:cytology* / Q000196:embryology; D006801:Humans; D051379:Mice; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013059:Spectrum Analysis, Raman / Q000379:methods*