title: Maximizing the adequacy of Hologic(®) Cervista(®) HPV HR results on ThinPrep(®) Pap samples treated with glacial acetic acid.
abstract: BACKGROUND
  Although glacial acetic acid (GAA) treatment of bloody cervical samples has reduced the rate of unsatisfactory Pap Tests, recent studies suggest that it may negatively impact high-risk (hr)-HPV test results. The objectives of this study were to compare the levels of genomic DNA between GAA treated and nontreated ThinPrep(®) samples using the Hologic(®) Cervista(®) HPV-HR assay and to compare the adequacy of the ThinPrep(®) Pap Test between aliquoted and nonaliquoted samples.
  
  
  METHODS
  Prior to GAA treatment, 2.5 ml of the cervical sample was prealiquoted from 102 bloody ThinPrep(®) vials. Both GAA treated and nontreated samples were analyzed for hr-HPV using the Cervista(®) HPV HR assay. The levels of genomic DNA were measured and compared between these samples. In addition, ThinPrep(®) Pap Test adequacy rates were calculated and compared on aliquoted and nonaliquoted cervical samples.
  
  
  RESULTS
  Of the 102 cervical samples, 95 (93%) nontreated aliquots contained satisfactory levels of genomic DNA as compared to 36 (35%) GAA-treated samples (p < 0.00001). Ninety-nine (97%) aliquoted cervical samples were satisfactory for cytologic evaluation as compared to 1,326 (96%) GAA treated samples from 2013 (nonaliquoted); not statistically significant (p = 0.7968).
  
  
  CONCLUSIONS
  The levels of genomic DNA were significantly decreased in GAA treated vs non-treated TP samples. Aliquoting from the TP sample prior to treatment with GAA enables accurate measurement of DNA without affecting the adequacy of the TP cytology slide.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: Aparna
authors.initials: A
authors.lastname: Mahajan
authors.affiliation: University of Wisconsin Hospital and Clinics, Madison, Wisconsin.
authors.forename: Erek
authors.initials: E
authors.lastname: Kucher
authors.affiliation: University of Wisconsin Hospital and Clinics, Madison, Wisconsin.
authors.forename: Wanda
authors.initials: W
authors.lastname: Hoefle
authors.affiliation: University of Wisconsin Hospital and Clinics, Madison, Wisconsin.
authors.forename: Shawna
authors.initials: S
authors.lastname: Engelhardt
authors.affiliation: University of Wisconsin Hospital and Clinics, Madison, Wisconsin.
authors.forename: Molly
authors.initials: M
authors.lastname: Accola
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: Samuel
authors.initials: S
authors.lastname: Barasch
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: William
authors.initials: W
authors.lastname: Rehrauer
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Selvaggi
mesh_terms: D019342:Acetic Acid; D061809:Human Papillomavirus DNA Tests / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D065006:Papanicolaou Test / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity
chemical_list: D019342:Acetic Acid
keywords: Hologic® Cervista® HPV HR test; ThinPrep® Pap test; adequacy rates; cervical cytology; glacial acetic acid