title: [The adhesive properties of two bonding systems to tetracycline stained dentin].
abstract: OBJECTIVE
  To investigate and compare the bonding properties of Single Bond 2 and SE Bond to tetracycline stained dentin in vitro.
  
  
  METHODS
  Ten extracted tetracycline stained human teeth and ten extracted normal human teeth were collected and the occlusal dentin surfaces of all extracted teeth were exposed. The tetracycline stained teeth and normal teeth were divided into two groups, respectively and randomly, based on the adhesives applied. Total-etch adhesive(Single Bond 2) and self-etch adhesive(SE Bond) were used. After application of the adhesives to the dentin surfaces, composite crowns were built up. After 24 h water storage, the teeth were sectioned longitudinally into sticks(0.9 mm×0.9 mm bonding area) for micro tensile testing or micro Raman spectroscopy detection. Bonding strength(μTBS) and resin conversion rate were analyzed using one-way ANOVA.
  
  
  RESULTS
  The tetracycline Single Bond 2 group presented lower bonding strength[(16.17 ± 3.16) MPa] than the tetracycline SE Bond group[(25.82 ± 2.62) MPa], and also demonstrated lower bonding strength than the normal Single Bond 2 group[(29.13 ± 2.44) MPa] and the normal SE Bond group[(24.29±2.83) MPa] (P<0.05) , while there was no statistical differences among the other three groups(P>0.05). The resin conversion rate of tetracycline Single Bond 2 group[(55±6)%] was significantly lower than the tetracycline SE Bond group[(66±3)% ](P<0.05) and also lower than the normal Single Bond 2 group[(64 ± 5)%] and the normal SE Bond group[(65 ± 4)%] (P<0.05). No statistically significant differences were observed among the other three groups(P>0.05).
  
  
  CONCLUSIONS
  The bonding strength of total-etch adhesive system to the tetracycline stained dentin was significantly lower than that to the normal dentin.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Liu
authors.forename: K N
authors.initials: KN
authors.lastname: Liang
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: L B
authors.initials: LB
authors.lastname: He
mesh_terms: D000269:Adhesives; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D003442:Crowns; D001840:Dental Bonding; D045662:Dental Etching; D003764:Dental Materials; D003804:Dentin; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D011897:Random Allocation; D013718:Tensile Strength; D013754:Tetracyclines; D014075:Tooth Discoloration
chemical_list: D000269:Adhesives; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D013754:Tetracyclines; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate