title: Possible target-related proteins and signal network of bufalin in A549 cells suggested by both iTRAQ-based and label-free proteomic analysis.
abstract: Bufalin (BF) exhibited antiproliferation and antimigration effects on human A549 lung cancer cells. To search its target-related proteins, protein expression profiles of BF-treated and control cells were compared using two quantitative proteomic methods, iTRAQ-based and label-free proteomic analysis. A total of 5428 proteins were identified in iTRAQ-based analysis while 6632 proteins were identified in label-free analysis. The number of common identified proteins of both methods was 4799 proteins. By application of 1.20-fold for upregulated and 0.83-fold for downregulated cutoff values, 273 and 802 differentially expressed proteins were found in iTRAQ-based and label-free analysis, respectively. The number of common differentially expressed proteins of both methods was 45 proteins. Results of bioinformational analysis using Metacore(TM) showed that the two proteomic methods were complementary and both suggested the involvement of oxidative stress and regulation of gene expression in the effects of BF, and fibronectin-related pathway was suggested to be an important pathway affected by BF. Western blotting assay results confirmed BF-induced change in levels of fibronectin and other related proteins. Overexpression of fibronectin by plasmid transfection ameliorated antimigration effects of BF. Results of the present study provided information about possible target-related proteins and signal network of BF.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.|Department of Pharmacy, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou, P. R. China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Feng
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.affiliation: AB Sciex, Shanghai, P. R. China.
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Jin
authors.affiliation: AB Sciex, Shanghai, P. R. China.
authors.forename: Ji
authors.initials: J
authors.lastname: Luo
authors.affiliation: Thermo Fisher Scientific, Shanghai, P. R. China.
authors.forename: Ai-Ying
authors.initials: AY
authors.lastname: Nie
authors.affiliation: Thermo Fisher Scientific, Shanghai, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Thomson Reuters, Shanghai, P. R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Wan-Ying
authors.initials: WY
authors.lastname: Wu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Jiang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Hu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002018:Bufanolides / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005353:Fibronectins; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D002018:Bufanolides; D005353:Fibronectins; D020543:Proteome; C022777:bufalin
keywords: A549 cells; Bufalin; Cell biology; Label-free; iTRAQ-based