title: Recombinant CP40 from Corynebacterium pseudotuberculosis confers protection in mice after challenge with a virulent strain.
abstract: BACKGROUND
  Caseous Lymphadenitis (CLA) is a contagious, infectious, chronic disease caused by Corynebacterium pseudotuberculosis, which affects mainly sheep and goats. The clinical prevalence of CLA in Brazil is 30%, resulting in decreased milk production, weight loss, and unusable meat and leather. Prophylaxis is based on vaccination; however, current vaccinations do not offer effective protection against the infection, which makes the development of a new vaccine essential to control this disease.
  
  
  METHODS
  Here, we developed a recombinant vaccine based on CP40 protein (rCP40) combined with an adjuvant (Freund's complete adjuvant or saponin) and evaluated its efficacy in a murine model of CLA. Female BALB/c mice were used in an immunization assay.
  
  
  RESULTS
  rCP40 induced high levels of IgG2a and IgG2b antibodies. After challenge with a virulent strain of C. pseudotuberculosis C57 (10(4)CFU/mL), the levels of IgG2a and IgG2b were sustained, indicating a Th1 response. The groups immunized with rCP40 protein (GES and GEF groups) showed 100% protection and was statistically significant in the GES and GEF groups (p<0.037 and p<0.0952, respectively).
  
  
  CONCLUSIONS
  The results indicated the recombinant protein CP40 induced an specific immune response in mice that was able to afford protection after challenge, regardless the adjuvant used in the formulation.
authors.affiliation: Instituto de Tecnologia e Pesquisa - Universidade Tiradentes, Avenida Murilo Dantas, 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Droppa-Almeida
authors.affiliation: Instituto de Tecnologia e Pesquisa - Universidade Tiradentes, Avenida Murilo Dantas, 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil.
authors.forename: Wanessa L P
authors.initials: WL
authors.lastname: Vivas
authors.affiliation: Instituto de Tecnologia e Pesquisa - Universidade Tiradentes, Avenida Murilo Dantas, 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil.
authors.forename: Katharina Kelly O
authors.initials: KK
authors.lastname: Silva
authors.affiliation: Centro de Biotecnologia - Universidade Federal de Pelotas, Campus Capão do Leão, Capão do Leão, Rio Grande do Sul 96010-900, Brazil.
authors.forename: Andrea F S
authors.initials: AF
authors.lastname: Rezende
authors.affiliation: Faculdade de Ciências Biológicas e Ambientais - Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, Km 12 - Cidade Universitária, Mato Grosso do Sul 79804-970, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Simionatto
authors.affiliation: Instituto de Ciências da Saúde - Universidade Federal da Bahia Avenida Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA 40110-100, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Meyer
authors.affiliation: Instituto de Tecnologia e Pesquisa - Universidade Tiradentes, Avenida Murilo Dantas, 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil.
authors.forename: Isabel B
authors.initials: IB
authors.lastname: Lima-Verde
authors.affiliation: Centro de Biotecnologia - Universidade Federal de Pelotas, Campus Capão do Leão, Capão do Leão, Rio Grande do Sul 96010-900, Brazil.
authors.forename: Odir
authors.initials: O
authors.lastname: Delagostin
authors.affiliation: Centro de Biotecnologia - Universidade Federal de Pelotas, Campus Capão do Leão, Capão do Leão, Rio Grande do Sul 96010-900, Brazil. Electronic address: fpadilha@yahoo.com.
authors.forename: Sibele
authors.initials: S
authors.lastname: Borsuk
authors.affiliation: Instituto de Tecnologia e Pesquisa - Universidade Tiradentes, Avenida Murilo Dantas, 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil. Electronic address: sibeleborsuk@gmail.com.
authors.forename: Francine F
authors.initials: FF
authors.lastname: Padilha
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D003354:Corynebacterium Infections / Q000517:prevention & control*; D016925:Corynebacterium pseudotuberculosis; D005260:Female; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic
keywords: BALB/c mice; Caseous Lymphadenitis; Corynebacterium pseudotuberculosis; Serine-protease