title: Over-expression of microRNA-375 inhibits papillary thyroid carcinoma cell proliferation and induces cell apoptosis by targeting ERBB2.
abstract: MicroRNAs (miRs) played important roles in the cell proliferation, apoptosis and other biological processes in cancer. In the present study we found that miR-375 was significantly down-regulated in human papillary thyroid carcinoma (PTC) tissues and cell lines. In this study we try to investigate the biological activity of miR-375 in human PTC cells and try to find the potential target of miR-375. Our study indicated that over-expression of miR-375 could inhibit the PTC cells proliferation and this inhibition was caused by the induction of cell apoptosis. In vivo animal study indicated that over-expression of miR-375 could significantly decrease the migration and invasion of human PTC cell in vivo. These results exhibit over-expression of miR-375 in human PTC cells could inhibit the process of human PTC. Further study demonstrated ERBB2 was a direct target of miR-375, over-expression of miR-375 decrease the both mRNA and protein expression of ERBB2 in human PTC cells. These data indicate miR-375 play important roles in the process and development of human PTC. These finds suggested that appropriate application of miR-375 regulation might be a new sight for the treatment of human PTC in the future.
authors.affiliation: Department III of General Surgery, The First Affiliated Hospital of Henan, University of Science and Technology, Luoyang City, Henan Province, PR China. Electronic address: xinzheng_w@126.com.
authors.forename: Xin-Zheng
authors.initials: XZ
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
authors.forename: Ya-Kai
authors.initials: YK
authors.lastname: Hang
authors.affiliation: Department III of General Surgery, The First Affiliated Hospital of Henan, University of Science and Technology, Luoyang City, Henan Province, PR China.
authors.forename: Jin-Biao
authors.initials: JB
authors.lastname: Liu
authors.affiliation: Department III of General Surgery, The First Affiliated Hospital of Henan, University of Science and Technology, Luoyang City, Henan Province, PR China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Hou
authors.affiliation: Department III of General Surgery, The First Affiliated Hospital of Henan, University of Science and Technology, Luoyang City, Henan Province, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department III of General Surgery, The First Affiliated Hospital of Henan, University of Science and Technology, Luoyang City, Henan Province, PR China.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D058990:Molecular Targeted Therapy; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy
chemical_list: D035683:MicroRNAs; D018719:Erb-b2 Receptor Tyrosine Kinases; C540933:MIRN375 microRNA, human; C508053:ERBB2 protein, human
keywords: Apoptosis; ERBB2; MicroRNA-375; Papillary thyroid carcinoma