title: Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551.
abstract: The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag than E. coli.
authors.affiliation: a Department of Biosciences, College of Science and Engineering , Kanto Gakuin University , Yokohama , Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nakayama
authors.affiliation: b Third Department of Forensic Science , National Research Institute of Police Science , Kashiwa , Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohmori
authors.affiliation: a Department of Biosciences, College of Science and Engineering , Kanto Gakuin University , Yokohama , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: a Department of Biosciences, College of Science and Engineering , Kanto Gakuin University , Yokohama , Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Iwata
authors.affiliation: b Third Department of Forensic Science , National Research Institute of Police Science , Kashiwa , Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Seto
authors.affiliation: b Third Department of Forensic Science , National Research Institute of Police Science , Kashiwa , Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawahara
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000067397:Nerve Agents / Q000378:metabolism*; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D042301:Sphingomonadaceae / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C471213:His-His-His-His-His-His; D000067397:Nerve Agents; D009842:Oligopeptides; D009943:Organophosphorus Compounds; D011993:Recombinant Fusion Proteins; D006639:Histidine; D043303:Aryldialkylphosphatase
keywords: Sphingobium amiense; Sphingobium fuliginis; nerve agent; organophosphorus hydrolase; paraoxon