title: Alteration of Natural Killer cell phenotype and function in obese individuals.
abstract: Obesity is associated with increased cancer rates and higher susceptibility to infections. The adipose tissue of obese individuals is inflammatory and may negatively impact on innate and adaptive immunity in a systemic way. Here, we explored the phenotype and function of peripheral Natural Killer (NK) cells of patients in correlation with their body mass index (BMI). We found that high BMI was associated with an increased activation status of peripheral NK cells, as measured by surface levels of CD69 and levels of granzyme-B. However, these activated NK cells had an impaired capacity to degranulate or to produce cytokines/chemokines when exposed to tumor cell lines deficient in MHC-I expression or coated with antibodies. This suggests that chronic stimulation of NK cells during obesity may lead to their incapacity to respond normally and eliminate target cells, which could contribute to the greater susceptibility of obese individuals to develop cancers or infectious diseases.
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie - International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Lyon, France; CNRS, UMR5308, Lyon, France; Laboratoire d'Immunologie, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, France. Electronic address: sebastien.viel@inserm.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Viel
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie - International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Lyon, France; CNRS, UMR5308, Lyon, France; Laboratoire d'Immunologie, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, France.
authors.forename: Laurie
authors.initials: L
authors.lastname: Besson
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie - International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Lyon, France; CNRS, UMR5308, Lyon, France; Laboratoire d'Immunologie, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, France.
authors.forename: Emily
authors.initials: E
authors.lastname: Charrier
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie - International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Lyon, France; CNRS, UMR5308, Lyon, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Marçais
authors.affiliation: Endocrinology, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Disse
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie - International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Lyon, France; CNRS, UMR5308, Lyon, France; Laboratoire d'Immunologie, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Bienvenu
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie - International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Lyon, France; CNRS, UMR5308, Lyon, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Walzer
authors.affiliation: Université Lyon 1, Lyon, France; INSERM U1052, CNRS 5286, Cancer Research Center of Lyon, Lyon, France; Hematology Laboratory, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Dumontet
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000276:immunology*; D010641:Phenotype; D055815:Young Adult
keywords: Body mass index; Cytotoxicity; Degranulation; Multiparameter FACS; Natural Killer cells; Obesity