title: Simultaneous scanning of two mice in a small-animal PET scanner: a simulation-based assessment of the signal degradation.
abstract: In PET imaging, research groups have recently proposed different experimental set ups allowing multiple animals to be simultaneously imaged in a scanner in order to reduce the costs and increase the throughput. In those studies, the technical feasibility was demonstrated and the signal degradation caused by additional mice in the FOV characterized, however, the impact of the signal degradation on the outcome of a PET study has not yet been studied. Here we thoroughly investigated, using Monte Carlo simulated [18F]FDG and [11C]Raclopride PET studies, different experimental designs for whole-body and brain acquisitions of two mice and assessed the actual impact on the detection of biological variations as compared to a single-mouse setting. First, we extended the validation of the PET-SORTEO Monte Carlo simulation platform for the simultaneous simulation of two animals. Then, we designed [18F]FDG and [11C]Raclopride input mouse models for the simulation of realistic whole-body and brain PET studies. Simulated studies allowed us to accurately estimate the differences in detection between single- and dual-mode acquisition settings that are purely the result of having two animals in the FOV. Validation results showed that PET-SORTEO accurately reproduced the spatial resolution and noise degradations that were observed with actual dual phantom experiments. The simulated [18F]FDG whole-body study showed that the resolution loss due to the off-center positioning of the mice was the biggest contributing factor in signal degradation at the pixel level and a minimal inter-animal distance as well as the use of reconstruction methods with resolution modeling should be preferred. Dual mode acquisition did not have a major impact on ROI-based analysis except in situations where uptake values in organs from the same subject were compared. The simulated [11C]Raclopride study however showed that dual-mice imaging strongly reduced the sensitivity to variations when mice were positioned side-by-side while no sensitivity reduction was observed when they were facing each other. This is the first study showing the impact of different experimental designs for whole-body and brain acquisitions of two mice on the quality of the results using Monte Carlo simulated [18F]FDG and [11C]Raclopride PET studies.
authors.forename: Anthonin
authors.initials: A
authors.lastname: Reilhac
authors.forename: Frédéric
authors.initials: F
authors.lastname: Boisson
authors.forename: Catriona
authors.initials: C
authors.lastname: Wimberley
authors.forename: Arvind
authors.initials: A
authors.lastname: Parmar
authors.forename: David
authors.initials: D
authors.lastname: Zahra
authors.forename: Hasar
authors.initials: H
authors.lastname: Hamze
authors.forename: Emma
authors.initials: E
authors.lastname: Davis
authors.forename: Andrew
authors.initials: A
authors.lastname: Arthur
authors.forename: Caroline
authors.initials: C
authors.lastname: Bouillot
authors.forename: Arnaud
authors.initials: A
authors.lastname: Charil
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Grégoire
mesh_terms: D000465:Algorithms; D000818:Animals; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D051379:Mice; D008962:Models, Theoretical; D049268:Positron-Emission Tomography / Q000295:instrumentation / Q000379:methods*; D020891:Raclopride / Q000493:pharmacokinetics; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D020891:Raclopride