title: Long noncoding RNA H19 inhibits the proliferation of fetal liver cells and the Wnt signaling pathway.
abstract: In this study, we found that H19 is the most strongly differentially expressed long noncoding RNA (lncRNA) during liver development. H19 may inhibit the proliferation of fetal liver cells by blocking the interaction between heterogeneous nuclear ribonucleoprotein (hnRNP) U and actin, which results in gene transcriptional repression. Based on ChIP-seq analysis, we found that genes involved in the Wnt signaling pathway are enriched among hnRNP U-binding genes. Further investigation demonstrated that hnRNP U has opposing effects on cell proliferation and Wnt/β-catenin signaling pathway activity compared to H19 and that hnRNP U is very important in this process.
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Shaobing
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Infectious Diseases, The Second Affiliated Hospital of Harbin Medical University, Heilongjiang, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Jihang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Infectious Diseases, The Second Affiliated Hospital of Harbin Medical University, Heilongjiang, China.
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Chuanchuan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Jinzhao
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Shuhan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Beijing Youan Hospital, Capital Medical University, Beijing, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D060449:Wnt Signaling Pathway / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics*
chemical_list: D000199:Actins; C094335:H19 long non-coding RNA; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D062085:RNA, Long Noncoding; D051176:beta Catenin
keywords: ChIP-seq; H19; Wnt/β-catenin signaling pathway; hnRNP U; liver development; long noncoding RNA