title: Chemical characterization of Pleurotus eryngii polysaccharide and its tumor-inhibitory effects against human hepatoblastoma HepG-2 cells.
abstract: This study was designed to investigate the chemical characterization and antitumor effects of Pleurotus eryngii polysaccharides (PEP). The crude PEP was fractionated into two fractions, namely PEP-1 and PEP-2. HPLC analysis showed that PEP-1 and PEP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 2.54×10(4)Da (PEP-1) and 4.63×10(5)Da (PEP-2), respectively. High molecular mass PEP-2 was shown to exhibit stronger growth inhibition against human hepatoblastoma HepG-2 cells in comparison with PEP-1. Flow cytometric analysis showed that PEP-2 exerted a stimulatory effect on apoptosis of HepG-2 cells, and induced the cell-cycle arrest at the S-phase, with the observation of intracellular ROS production. These findings suggest that the polysaccharides, especially PEP-2, are very important nutritional ingredients responsible for the anticancer health benefits of P. eryngii.
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Daoyuan
authors.initials: D
authors.lastname: Ren
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China. Electronic address: yuanli112086@snnu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China. Electronic address: xbyang@snnu.edu.cn.
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D056945:Hep G2 Cells; D018197:Hepatoblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008970:Molecular Weight; D020076:Pleurotus / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D053148:Caspase 3; D053453:Caspase 9
keywords: Anti-proliferation; Apoptosis; Cell cycle; Pleurotus eryngii; Polysaccharide; Purification