title: Preparation and characterization of bioactive composite scaffolds from polycaprolactone nanofibers-chitosan-oxidized starch for bone regeneration.
abstract: The objective of this study was to fabricate and investigate the characteristics of a suitable scaffold for bone regeneration. Therefore, chitosan was combined with various amounts of oxidized starch through reductive alkylation process. Afterwards, chopped CaP-coated PCL nanofibers were added into the chitosan-starch composite scaffolds in order to obtain bioactivity and mimic bone extracellular matrix structure. Scanning electron microscopy confirmed that all scaffolds had well-interconnected porous structure. The mean pore size, porosity, and water uptake of the composite scaffolds increased by incorporation of higher amounts of starch, while this trend was opposite for compressive modulus and strength. Osteoblast-like cells (MG63) culturing on the scaffolds demonstrated that higher starch content could improve cell viability. Moreover, the cells spread and anchored well on the scaffolds, on which the surface was covered with a monolayer of cells.
authors.affiliation: Department of Life science Engineering, Faculty of New Sciences and Technologies University of Tehran, P.O. Box 14395-1561, Tehran, Iran. Electronic address: J_nourmohammadi@ut.ac.ir.
authors.forename: Jhamak
authors.initials: J
authors.lastname: Nourmohammadi
authors.affiliation: Department of Life science Engineering, Faculty of New Sciences and Technologies University of Tehran, P.O. Box 14395-1561, Tehran, Iran. Electronic address: ghaee@ut.ac.ir.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Ghaee
authors.affiliation: Department of Life science Engineering, Faculty of New Sciences and Technologies University of Tehran, P.O. Box 14395-1561, Tehran, Iran. Electronic address: samira-hoseini@ut.ac.ir.
authors.forename: Samira Hosseini
authors.initials: SH
authors.lastname: Liavali
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013213:Starch / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D014867:Water; C016240:polycaprolactone; D013213:Starch; D048271:Chitosan; C020243:calcium phosphate
keywords: Bone; Calcium phosphate; Chitosan; Oxidized starch; Polycaprolactone; Scaffold