title: Characterization of a Reuterin-Producing Lactobacillus reuteri BPL-36 Strain Isolated from Human Infant Fecal Sample.
abstract: A reuterin (3-hydroxypropinaldehyde, 3-HPA)-producing isolate from a human infant fecal sample was identified as Lactobacillus reuteri BPL-36 strain. The organism displayed a broad-spectrum antimicrobial activity. The gene (gdh) encoding a glycerol dehydratase subunit was detected by PCR, thus confirming its reuterin-producing ability. Reuterin concentration of 89.63 mM/mL was obtained in the MRS-glycerol medium after 16 h of incubation at 37 °C. The reuterin concentration required to inhibit the growth of Pseudomonas aeruginosa, Escherichia coli O157: H7, Salmonella typhi, Staphylococcus aureus, and Listeria monocytogenes was found to be 1.0, 2.0, 2.0, 4.0, and 10.0 AU/mL, respectively. Antimicrobial efficiency test using BPL-36 cell-free supernatant co-incubated along with different test pathogens was done. Viability of all the tested pathogens decreased with increasing contact time with the cell-free supernatant. S. typhi was observed to be the most susceptible among the tested organisms, and the number of viable cells hugely declined as the contact with cell-free supernatant continued, resulting in a reduction of 6 log cycles (100 % inhibition) of the cells after 4 h of treatment. Production of biogenic amines and degradation of mucin by the reuterin-producing BPL-36 strain were not detected.
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India. rkm.micro@gmail.com.
authors.forename: R K
authors.initials: RK
authors.lastname: Malik
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: G
authors.initials: G
authors.lastname: Manju
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Pandey
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Garima
authors.initials: G
authors.lastname: Singroha
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Behare
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: J K
authors.initials: JK
authors.lastname: Kaushik
keywords: Antimicrobials; Lactobacillus reuteri; Pathogens; Reuterin; Safety