title: Functional characterization and analysis of the Arabidopsis UGT71C5 promoter region.
abstract: In the present study, we isolated an Arabidopsis promoter, UGT71C5, and analyzed its role in the regulation of the light response mechanism. We constructed a fusion vector pBI121-pU-GUS by integrating the UGT71C5 promoter upstream of the GUS reporter gene in pBI121, and then transferred this vector into Arabidopsis plants. The GUS activity of the transgenic plants was detected using a spectrophotometer under normal growth conditions as well as under light, drought, and ABA stress-treatments. The obtained results indicated that the GUS activity of transgenic plants ranged in between the activities observed in wild-type and 35S transgenic plants, which were used as positive control. Light stress for 8 and 12 h increased the GUS activity in transgenic plants by 3 and 4 times, respectively, compared to the activity in these plants under normal conditions. No such change in the GUS activity was observed under drought and ABA-treated conditions. This suggests that the UGT71C5 promoter is light inducible. Our study provides helpful insights into the elucidation of inducible promoters in Arabidopsis and the molecular mechanisms of light response.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.|State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, Sichuan, China.|Department of Biotechnology, Chengdu Institute of Biological Products, Chengdu, Sichuan, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Longquanyi District Bureau of Agriculture Development, Chengdu, Sichuan, China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Luo
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Li
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.|State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D060054:Agrobacterium / Q000235:genetics; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005964:Glucosyltransferases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008027:Light; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000528:radiation effects*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D000040:Abscisic Acid; D005964:Glucosyltransferases; C000604705:UGT71C5 protein, Arabidopsis; D005966:Glucuronidase