title: Prospective evaluation of the chromogenic medium CandiSelect 4 for differentiation and presumptive identification of non-Candida albicans Candida species.
abstract: Rapid identification of pathogenic yeasts is a crucial step in timely and appropriate antifungal therapy. For diagnostics in the clinical laboratory, simplified alternatives to barcoding are needed. CandiSelect 4 (CS4) medium, a chromogenic medium for isolation of clinical yeasts, allows routine recognition of Candida albicans and presumptive identification of Candida tropicalis, Candida glabrata, and Candida krusei. We evaluated an extension of this method with 46 non-Candida albicans Candida (NCAC) and 7 Malassezia species. The medium supported growth of all species tested and a wide diversity of cultural types were observed. Colony colours were in violet, turquoise (including green and blue), or white tinges. Eight NCAC species produced violet pigmentation similar to that of C. albicans. Most NCAC species, including C. glabrata and C. tropicalis were distributed in the turquoise group. Malassezia species were invariably blue.
authors.affiliation: Department of Microbiology, Guizhou Medical University, 550004 Guiyang, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, 3584 CT Utrecht, The Netherlands.
authors.forename: G Sybren
authors.initials: GS
authors.lastname: de Hoog
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, 3584 CT Utrecht, The Netherlands.
authors.forename: Akke
authors.initials: A
authors.lastname: Cornelissen
authors.affiliation: Department of Microbiology, Guizhou Medical University, 550004 Guiyang, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lyu
authors.affiliation: Department of Microbiology, Guizhou Medical University, 550004 Guiyang, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Mou
authors.affiliation: Department of Microbiology, Guizhou Medical University, 550004 Guiyang, China.
authors.forename: Taohua
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Microbiology, Guizhou Medical University, 550004 Guiyang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, 3584 CT Utrecht, The Netherlands.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Vatanshenassan
authors.affiliation: Department of Microbiology, Guizhou Medical University, 550004 Guiyang, China; CBS-KNAW Fungal Biodiversity Centre, 3584 CT Utrecht, The Netherlands. Electronic address: joycekangtokyo@qq.com.
authors.forename: Yingqian
authors.initials: Y
authors.lastname: Kang
mesh_terms: D002175:Candida / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D002176:Candida albicans / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D016533:Mycological Typing Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D003470:Culture Media
keywords: Candida diagnostics; Chromogenic media; Malassezia; NCAC; Non-Candida albicans Candida