title: Towards proteomic species barcoding of fungi - An example using Scedosporium/Pseudallescheria complex isolates.
abstract: MALDI-ToF mass spectrometry offers fast and reliable species identification for bacteria and yeasts under clinical routine conditions. Here, we produced mass spectra for identification of clinically important species of the Pseudallescheria/Scedosporium complex using the recently suggested new nomenclature and use this example to discuss to what extent the principle of DNA barcoding might be transferred to mass spectrometry.
authors.affiliation: Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Bernhard
authors.affiliation: Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Andreas Erich
authors.initials: AE
authors.lastname: Zautner
authors.affiliation: Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, D-45122 Essen, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Steinmann
authors.affiliation: Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Weig
authors.affiliation: Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Groß
authors.affiliation: Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany. Electronic address: obader@gwdg.de.
authors.forename: Oliver
authors.initials: O
authors.lastname: Bader
mesh_terms: D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009181:Mycoses / Q000382:microbiology*; D010802:Phylogeny; D040901:Proteomics / Q000379:methods*; D011541:Pseudallescheria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D021681:Scedosporium / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal
keywords: Lomentospora prolificans; MALDI BioTyper; Scedosporium apiospermum complex; Scedosporium boydii