title: Oriented Protein Nanoarrays on Block Copolymer Template.
abstract: Here, a simple yet robust method is developed to fabricate oriented protein nanoarrays by employing a block copolymer (BCP) template, which presents nano-scaled spot areas at high-density arrays. Unlike the conventional BCP nanolithography, the BCP platform described here resists nonspecific protein adsorption and prevents the denaturation of immobilized proteins in aqueous solution. The orderly arranged array areas are functionalized by linking chemistry which allows for the precise control of protein orientation. This approach allows us to generate potentially oriented protein nanoarrays at high-density array spots, which is useful for miniaturized nanoarrays within high-throughput proteomic applications.
authors.affiliation: Key Laboratory of Materials Chemistry for Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, 430074, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Materials Chemistry for Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, 430074, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000327:Adsorption; D049452:Green Fluorescent Proteins / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D008689:Methacrylates; D011137:Polystyrenes; D011145:Polyvinyls; C521882:poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer; C541965:polystyrene-b-poly(ethylene oxide); C522509:polystyrene-block-poly(methyl methacrylate); D014867:Water; D049452:Green Fluorescent Proteins; D011102:Polyhydroxyethyl Methacrylate; D011092:Polyethylene Glycols
keywords: block copolymer; immobilization; orientation; protein nanoarray