title: Enhanced photoelectrochemical aptasensing platform for TXNDC5 gene based on exciton energy transfer between NCQDs and TiO2 nanorods.
abstract: The over expression of thioredoxin domain-containing protein 5 (TXNDC5) can promote the growth of castration-resistant prostate cancer (CRPC). A novel highly sensitive photoelectrochemical (PEC) aptsensor was developed for the detection of TXNDC5 by using the nanohybrids (TiO2 NRs/NCQDs) of nitrogen-doped carbon quantum dots (NCQDs) and TiO2 nanorods as the photo-to-electron conversion medium. TiO2 NRs/NCQDs nanohybrids were prepared by controlling the experimental condition. TiO2 NRs were self-assembled to form the nanopores with good photocurrent conversion efficiency. NCQDs possessed carboxyl groups (-COOH) and amino groups (-NH2) in the preparation process. -COOH and -NH2 groups played important roles for anchoring the capture probes (5' primer and 3' primer) through covalent binding. The ultrasensitive and stable detection for TXNDC5 was achieved by the specific recognition between the capture probes and the targets. The fabricated aptsensor showed excellent performance with a wide linear range (0.5 fmol/L ∼ 10 nmol/L) and a low detection limit of 0.1 fmol/L. This kind of aptsensor would provide a potential application for TXNDC5.
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: Shandong Medicinal Biotechnology Centre, the Key Lab for Biotechnology Drugs of Ministry of Health, the Key Lab of Rare and Uncommon Disease, Jinan 250022, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shandong Medicinal Biotechnology Centre, the Key Lab for Biotechnology Drugs of Ministry of Health, the Key Lab of Rare and Uncommon Disease, Jinan 250022, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Pan
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei