title: Novel Asymmetric Wettable AgNPs/Chitosan Wound Dressing: In Vitro and In Vivo Evaluation.
abstract: A novel silver nanoparticles (AgNPs)/chitosan composite dressing with asymmetric wettability surfaces was successfully prepared via a simple two-step method for biomedical applications as wound healing materials. First, AgNPs were assembled into the chitosan sponge which was prepared by lyophilization process. Then one side of the sponge was modified by a thin layer of stearic acid. The incorporation of AgNPs into chitosan dressing could enhance the antibacterial activity against drug-sensitive and drug-resistant pathogenic bacteria. The asymmetric surface modification endows the dressing with both highly hydrophobic property and inherent hydrophilic nature of chitosan. The hydrophobic surface of the dressing shows waterproof and antiadhesion for contaminant properties, whereas the hydrophilic surface preserves its water-absorbing capability and efficiently inhibits the growth of bacteria. Furthermore, the AgNPs/chitosan composite dressing displays improved moisture retention and blood clotting ability compared to the unmodified dressings. Cytocompatibility test evaluated in vitro and in a wound infection model illustrates the nontoxic nature of the composite dressing. More importantly, the in vivo wound healing model evaluation in mice reveals that the asymmetric AgNPs/chitosan dressing promotes the wound healing and accelerates the reepithelialization and collagen deposition. The silver accumulation in mice body treated by the composite dressing is far lower than that of the clinically used Acasin nanosilver dressing treated mice. This work indicates the huge potential of the novel AgNPs/chitosan wound dressing with asymmetrical wettability for clinical use.
authors.affiliation: School of Chemistry and Environmental Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology , Xiongchu Avenue, Wuhan 430073, PR China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Liang
authors.affiliation: School of Chemistry and Environmental Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology , Xiongchu Avenue, Wuhan 430073, PR China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: School of Chemistry and Environmental Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology , Xiongchu Avenue, Wuhan 430073, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Chemistry and Environmental Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology , Xiongchu Avenue, Wuhan 430073, PR China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Gao
authors.affiliation: School of Chemistry and Environmental Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology , Xiongchu Avenue, Wuhan 430073, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
mesh_terms: D000818:Animals; D001458:Bandages; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D017155:Wettability; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy
chemical_list: D012834:Silver; D048271:Chitosan
keywords: antiadhesion; antibacterial activity; asymmetric wettability; chitosan dressing; in vivo; silver nanoparticle