title: A Two-Photon Fluorescent Probe for Lysosomal Thiols in Live Cells and Tissues.
abstract: Lysosome-specific fluorescent probes are exclusive to elucidate the functions of lysosomal thiols. Moreover, two-photon microscopy offers advantages of less phototoxicity, better three dimensional spatial localization, deeper penetration depth and lower self-absorption. However, such fluorescent probes for thiols are still rare. In this work, an efficient two-photon fluorophore 1,8-naphthalimide-based probe conjugating a 2,4-dinitrobenzenesulfonyl chloride and morpholine was designed and synthesized, which exhibited high selectivity and sensitivity towards lysosomal thiols by turn-on fluorescence method quantitatively and was successfully applied to the imaging of thiols in live cells and tissues by two-photon microscopy.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Han
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D000818:Animals; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008813:Mice, Inbred ICR; D055620:Optical Phenomena; D009928:Organ Specificity; D017785:Photons; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds