title: The Orientations of Large Aspect-Ratio Coiled-Coil Proteins Attached to Gold Nanostructures.
abstract: Methods for patterning biomolecules on a substrate at the single molecule level have been studied as a route to sensors with single-molecular sensitivity or as a way to probe biological phenomena at the single-molecule level. However, the arrangement and orientation of single biomolecules on substrates has been less investigated. Here, the arrangement and orientation of two rod-like coiled-coil proteins, cortexillin and tropomyosin, around patterned gold nanostructures is examined. The high aspect ratio of the coiled coils makes it possible to study their orientations and to pursue a strategy of protein orientation via two-point attachment. The proteins are anchored to the surfaces using thiol groups, and the number of cysteine residues in tropomyosin is varied to test how this variation affects the structure and arrangement of the surface-attached proteins. Molecular dynamics studies are used to interpret the observed positional distributions. Based on initial studies of protein attachment to gold post structures, two 31-nm-long tropomyosin molecules are aligned between the two sidewalls of a trench with a width of 68 nm. Because the approach presented in this study uses one of twenty natural amino acids, this method provides a convenient way to pattern biomolecules on substrates using standard chemistry.
authors.affiliation: Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Jae-Byum
authors.initials: JB
authors.lastname: Chang
authors.affiliation: Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 440-746, South Korea.|Department of Chemistry, Sungkyunkwan University, Suwon, 440-746, South Korea.
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Evan
authors.initials: E
authors.lastname: Thompson
authors.affiliation: Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 440-746, South Korea.
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: No
authors.affiliation: Department of Chemistry, Sungkyunkwan University, Suwon, 440-746, South Korea.
authors.forename: Nam Hyeong
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Arrieta
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Vitor R
authors.initials: VR
authors.lastname: Manfrinato
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Keating
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Karl K
authors.initials: KK
authors.lastname: Berggren
mesh_terms: D006046:Gold / Q000737:chemistry*; D006801:Humans; D056004:Molecular Dynamics Simulation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013696:Temperature; D014335:Tropomyosin / Q000737:chemistry
chemical_list: D011506:Proteins; D014335:Tropomyosin; D006046:Gold
keywords: cortexillin; gold nanostructures; nanofabrication; protein patterning; tropomyosin