title: Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery.
abstract: Sepsis is the leading cause of death for critical ill patients and an essential focus in immunopharmacological research. The cecal ligation and puncture (CLP) model is regarded as a golden standard model for sepsis study. However, this animal model is easily affected by variability problems and dramatically affects pharmacological evaluation of anti-sepsis therapies, which requires standardized procedures and stable outcomes. Herein, the traditional syringe needle based puncture method was used as the major unstable factor for CLP models. Syringe needles created varied mortality in parallel experimental groups of CLP rats; they were inconsistent for severity control as mortality in CLP rats was not correlated with change in punctures, ligation lengths, or needle sizes. Moreover, the use of drainage tubes or strips, which was supposed to strengthen drainage stability, also failed to improve consistency of traditional syringe needles. To solve the consistency problem, an improved design of CLP surgery by puncture with newly-developed three-edged needles was described herein. In contrast to traditional syringe needles, these three-edged needles ensured more stable outcomes in repetitive groups. Furthermore, increased severity was found to be consistent with the enlarged needle size, as shown by the elevated mortality, increased proinflammatory cytokines, abnormal coagulation, worsen acidosis and more severe acute lung injury. In conclusion, application of the newly-developed three-edged needles provides a simple and feasible method to improve stability when conducting CLP surgery, which is significant for pharmacological studies on sepsis.
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Fan
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Yongling
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing, 400038, PR China. Electronic address: zhouh64@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, PR China. Electronic address: zhengj99219@163.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D055371:Acute Lung Injury / Q000097:blood / Q000209:etiology / Q000473:pathology; D000818:Animals; D002432:Cecum / Q000293:injuries / Q000601:surgery; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004867:Equipment Design; D008026:Ligation; D008070:Lipopolysaccharides / Q000097:blood; D008168:Lung / Q000473:pathology; D008297:Male; D009339:Needles; D011677:Punctures; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D018805:Sepsis / Q000097:blood / Q000150:complications / Q000473:pathology
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides
keywords: Animal model; Cecal ligation and puncture; Inconsistency; Sepsis; Syringe needles; Three-edged needles