title: Expression of the Mucus Adhesion Gene Mub, Surface Layer Protein Slp and Adhesion-Like Factor EF-TU of Lactobacillus acidophilus ATCC 4356 Under Digestive Stress Conditions, as Monitored with Real-Time PCR.
abstract: Expression of the mucus adhesion gene Mub, surface layer protein Slp and adhesion-like factor EF-Tu by Lactobacillus acidophilus ATCC 4356 grown in the presence of mucin, bile and pancreatin and at low pH was studied using real-time PCR. None of the genes were up-regulated under increasing concentrations of mucin, while Slp and EF-Tu were up-regulated in the presence of bile and pancreatin at normal concentrations (0.3%, w/v) and under stress conditions (1.0%, w/v).
authors.affiliation: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
authors.forename: K
authors.initials: K
authors.lastname: Ramiah
authors.affiliation: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
authors.forename: C A
authors.initials: CA
authors.lastname: van Reenen
authors.affiliation: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. lmtd@sun.ac.za.
authors.forename: L M T
authors.initials: LM
authors.lastname: Dicks
keywords: Lactobacillus acidophilus; Mub, Slp and EF-Tu expression; Real-time PCR