title: Ampullimonas aquatilisgen. nov., sp. nov. isolated from bottled mineral water.
abstract: Two isolates, designated B15.09-116Tand B15.09-124, were recovered from bottled mineral water in Portugal. Based on 16S rRNA gene sequence analysis, these strains were related most closely to species of the genusDerxia(belonging to the familyAlcaligenaceae) with pairwise sequence similarities of 93.0-93.6 %. The isolates were not pigmented and formed Gram-stain-negative, short, motile rod-shaped cells. The organisms were strictly aerobic, oxidase-positive and catalase-negative. These organisms also fixed N2. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 was the major respiratory quinone. The DNA G+C content of strain B15.09-116Twas 49.8 mol%. Based on phylogenetic, physiological and biochemical characteristics the two strains are considered to represent a novel species of a new genus, for which the nameAmpullimonas aquatilisgen. nov., sp. nov. is proposed. The type strain ofAmpullimonas aquatilisis B15.09-116T( = CECT 8581T= LMG 28208T).
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: França
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
authors.forename: Luciana
authors.initials: L
authors.lastname: Albuquerque
authors.affiliation: Instituto Nacional de Investigação Agrária e Veterinária, Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal.
authors.forename: Claudia
authors.initials: C
authors.lastname: Sánchez
authors.affiliation: Instituto Nacional de Investigação Agrária e Veterinária, Av. da República, Quinta do Marquês, 2780-159 Oeiras, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Fareleira
authors.affiliation: Microbiology Unit, BIOCANT Biotechnological Park, 3060-197 Cantanhede, Portugal.|Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa