title: CXCL12/CXCR4 Axis Improves Migration of Neuroblasts Along Corpus Callosum by Stimulating MMP-2 Secretion After Traumatic Brain Injury in Rats.
abstract: To investigate the effect of CXCL12 on migration of neural precursor cells after traumatic brain injury (TBI). We randomly divided 48 rats into four groups: (1) the sham group, rats were performed craniotomy only, (2) the control group, saline were injected into the ipsilateral cortex after TBI, (3) the CXCL12 group, CXCL12 were injected into the ipsilateral cortex after TBI, and (4) the CXCL12 + AMD3100 group, CXCL12 and AMD3100 were mixed together and injected into the ipsilateral cortex after TBI. At 7 days after TBI, the brain tissues were subjected to immunofluorescent double-labeled staining with the antibodies of CXCR4/DCX, MMP-2/DCX, MMP-2/GFAP, MMP-2/NeuN. Western blot assay was used to measure the protein levels of MMP-2. Compared with the control group, the number of CXCR4/DCX and MMP-2 positive cells around the injured corpus callosum area were significantly increased in the CXCL12 treatment group. The area occupied by these cells expanded and the shape changed from chain distribution to radial. CXCL12 + AMD3100 treatment significantly decreased the number and distribution area of CXCR4/DCX and MMP-2 positive cells compared with the CXCL12 treatment and control group. The DCX positive cells could not form chain or radial distribution. The protein expressions of MMP-2 had the similar change trends as the results of immunofluorescent staining. MMP-2 could be secreted by DCX, GFAP and NeuN positive cells. CXCL12/CXCR4 axis can improve the migration of the neuroblasts along the corpus callosum by stimulating the MMP-2 secretion of different types of cells.
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Mao
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Qin
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Tian
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China. jguohua@163.com.
authors.forename: Guohua
authors.initials: G
authors.lastname: Jin
mesh_terms: D000818:Animals; D001596:Benzylamines; D000070642:Brain Injuries, Traumatic / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000008:administration & dosage*; D003337:Corpus Callosum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D000080027:Cyclams; D000089662:Doublecortin Protein; D006571:Heterocyclic Compounds / Q000008:administration & dosage; D007276:Injections, Intraventricular; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D001596:Benzylamines; C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D000080027:Cyclams; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; D020778:Matrix Metalloproteinase 2; C088327:plerixafor
keywords: CXCL12; CXCR4; MMP-2; Migration; Traumatic brain injury