title: Methodological considerations for using umu assay to assess photo-genotoxicity of engineered nanoparticles.
abstract: In this study we investigated the feasibility of high-throughput (96-well plate) umu assay to test the genotoxic effect of TiO2 engineered nanoparticles (ENPs) under UV light (full spectrum) and visible light (455 nm). Exposure of TiO2 ENPs to up to 60 min of UV light induced a photocatalytic production of ROS. However, UV light itself caused cytotoxic damage to Salmonella typhimurium at exposures >15 min and a genotoxic effect at exposures >0.5 min; and use of UV filters did not lower this effect. No genotoxicity of TiO2 ENPs was observed under visible light conditions at concentrations up to 100 μg mL(-1); or under dark conditions at concentrations up to 667 μg mL(-1), though cytotoxicity was seen at the higher concentrations. Additionally, the growth factor calculation was influenced by a shading effect due to ENPs, and was corrected by considering the pre-incubation OD readings of Plate B. Recommendations provided in this paper, as well as investigation of the effect of the light sources should be considered when using the umu assay to quantify the photo-genotoxicity of engineered nanomaterials.
authors.affiliation: Department of Environmental Engineering, Miljoevej Building 113, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Denisa
authors.initials: D
authors.lastname: Cupi
authors.affiliation: Department of Environmental Engineering, Miljoevej Building 113, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. Electronic address: denc@env.dtu.dk.
authors.forename: Anders
authors.initials: A
authors.lastname: Baun
mesh_terms: D004249:DNA Damage / Q000187:drug effects*; D005240:Feasibility Studies; D009152:Mutagenicity Tests; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D014025:Titanium / Q000737:chemistry / Q000633:toxicity; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium
keywords: Engineered nanoparticles; Methodological considerations; Photo-genotoxicity; umu assay