title: [Porphyromonas gingivalis FimA genotype distribution among periodontitis patients with type 2 diabetes].
abstract: OBJECTIVE
  To investigate the distribution differences of Porphyromonas gingivalis(Pg) FimA genotypes between periodontitis patients with and without type 2 diabetes for a better understanding of the relationship between diabetes and periodontitis.
  
  
  METHODS
  Questionnaires and detailed periodontal examinations(6 sites per tooth) were conducted in 80 subjects with moderate-severe chronic periodontitis in the Department of Periodontology, Peking University School and Hospital of Stomatology. There were 40 type 2 diabetic patients and 40 systemicly healthy patients enrolled respectively. The periodontal parameters including plaque index(PLI), probing depth(PD), bleeding index(BI), attachment loss(AL) by 6 sites per tooth and numbers of missing teeth were also recorded. Pooled subgingival plaque samples using pocket method with Whatman No3 filterpaper were collectedat each of the 6 sites from one incisor and one molar. Pg and its FimA genotype distributions were investigated using DNA extrctedfrom plaque samples by PCR.
  
  
  RESULTS
  Diabetic patients had a significantly higher score of PLI[2.35(0.58) vs 1.64(0.76),P<0.05] , while rest of periodontal indexes observed(PD, BI and AL) did not differ significantly between diabetic patients and systemicly healthy controls(P>0.05). The detection rate of Pg did not show statistically significant difference between the two groups(50% vs 60%,P>0.05). However, the proportion of FimA Ⅱ was significantly higher in diabetic group than systemicly healthy group(80% vs 42%,P<0.05).
  
  
  CONCLUSIONS
  Type 2 diabetic patients were prone to be infected by highly virulent strains of Pg: FimA ⅡPg.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: B T
authors.initials: BT
authors.lastname: Yang
authors.affiliation: Department of Stomatology, Aviation General Hospital of China Medical University, Beijing 100012, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Xu
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: L
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Meng
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
mesh_terms: D016866:Bacteroidaceae Infections; D055113:Chronic Periodontitis / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D003774:Dental Plaque Index; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010512:Periodontal Index; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000302:isolation & purification