title: Change of Th17 Lymphocytes and Treg/Th17 in Typical and Atypical Optic Neuritis.
abstract: BACKGROUND
  Typical and atypical optic neuritis (ON) are two clinical types of autoimmune inflammatory diseases of the optic nerve that causes acute vision loss, and are difficult to distinguish in their early stages. The disturbance in the balance of Th17 and Treg lymphocytes is thought to play an essential role in these autoimmune inflammatory diseases.
  
  
  OBJECTIVE
  To detect the clinical relevance of Th17 and Treg in peripheral blood and the ratio of Treg/Th17 in patients with typical and atypical ON. To determine whether analysis of Th17 and Treg lymphocytes will provides insights into the different disease phenotypes of typical and atypical ON.
  
  
  METHODS
  We studied a consecutive series of patients aged 14-70 years who presented to our neurological department with typical ON (n = 30) or atypical ON (n = 33) within 4 weeks of their acute attacks. Routine clinical tests and ophthalmological examination were performed in all patients. Blood samples were collected from untreated patients and from gender- and age-matched healthy controls (n = 30). The proportion of peripheral blood Th17 cells and Treg cells was determined by flow cytometry.
  
  
  RESULTS
  Patients with atypical ON had a higher proportion of Th17 cells than patients with typical ON (3.61 ± 1.56 vs 2.55 ± 1.74, P<0.01) or controls (1.45 ± 0.86, P<0.01). The proportion of Th17 cells in patients with typical ON was also markedly higher than in controls (P<0.01). The mean percentage of Treg cells in atypical ON (6.31 ± 2.11) and typical ON (6.80 ± 2.00) were significantly lower when compared to controls (8.29 ± 2.32, both P<0.01). No significant difference in Treg frequency was observed between typical ON and atypical ON (p>0.05).
  
  
  CONCLUSIONS
  The frequency of Th17 cells is higher in atypical ON than typical ON, and patients with atypical ON have a greater imbalance of pro-inflammatory and regulatory cells than patients with typical ON when compared with controls. These changes are indicative of distinct pathological mechanisms and may provide useful information to distinguish typical and atypical ON.
authors.affiliation: Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Hengri
authors.initials: H
authors.lastname: Cong
authors.affiliation: Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Hanqiu
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Cui
authors.affiliation: Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009902:Optic Neuritis / Q000097:blood*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*; D058504:Th17 Cells / Q000166:cytology*