title: Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging.
abstract: Cells are complex chemical systems, where the molecular composition at different cellular locations and specific intracellular chemical interactions determine the biological function. An in-situ nondestructive characterization of the complicated chemical processes (like e.g. apoptosis) is the goal of our study. Here, we present the results of simultaneous and three-dimensional imaging of double organelles (nucleus and membrane) in single HeLa cells by means of either labelled or label-free surface-enhanced Raman spectroscopy (SERS). This combination of imaging with and without labels is not possible when using fluorescence microscopy. The SERS technique is used for a stereoscopic description of the intrinsic chemical nature of nuclei and the precise localization of folate (FA) and luteinizing hormone-releasing hormone (LHRH) on the membrane under highly confocal conditions. We also report on the time-dependent changes of cell nuclei as well as membrane receptor proteins during apoptosis analyzed by statistical multivariate methods. The multiplex three-dimensional SERS imaging technique allows for both temporal (real time) and spatial (multiple organelles and molecules in three-dimensional space) live-cell imaging and therefore provides a new and attractive 2D/3D tracing method in biomedicine on subcellular level.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Xiangru
authors.initials: X
authors.lastname: Bai
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Le
authors.initials: L
authors.lastname: Su
authors.affiliation: College of Computer Science and Technology, Jilin University, Changchun 130012 (China).
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Du
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
authors.affiliation: Department of Physics &Earth Sciences, Focus Area Health, Jacobs University Bremen, Campus Ring 1, 28759 Bremen (Germany).
authors.forename: Arnulf
authors.initials: A
authors.lastname: Materny
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors.forename: Jiming
authors.initials: J
authors.lastname: Hu
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D058948:Cell Tracking; D003593:Cytoplasm / Q000648:ultrastructure; D005492:Folic Acid / Q000737:chemistry / Q000302:isolation & purification; D006046:Gold / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D053768:Metal Nanoparticles / Q000737:chemistry; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D013059:Spectrum Analysis, Raman
chemical_list: D007987:Gonadotropin-Releasing Hormone; D006046:Gold; D005492:Folic Acid