title: Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots.
abstract: The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Jiskrová
authors.affiliation: Department of Metabolomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Novák
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Pospíšilová
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Holubová
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic; Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå SE-90183, Sweden.
authors.forename: Michal
authors.initials: M
authors.lastname: Karády
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Galuszka
authors.affiliation: Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå SE-90183, Sweden.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Robert
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic. Electronic address: ivo.frebort@upol.cz.
authors.forename: Ivo
authors.initials: I
authors.lastname: Frébort
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D002451:Cell Compartmentation; D003583:Cytokinins / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D001467:Hordeum / Q000254:growth & development / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D026901:Membrane Transport Proteins; D010937:Plant Growth Regulators; C560559:Wat1 protein, Arabidopsis