title: Fingerprint Analysis of Desmodium Triquetrum L. Based on Ultra Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometrics Methods.
abstract: A fingerprinting approach was developed by means of ultra high-performance liquid chromatography with photodiode array detector for the quality control of Desmodium triquetrum L., an herbal medicine widely used for clinical purposes. Ten batches of raw material samples of D. triquetrum were collected from different regions of China. All UPLC analyses were carried out on a Waters ACQUITY UPLC BEH shield RP18 column (2.1 × 50 mm, 1.7 µm particle size) at 60°C, with a gradient mobile phase composed of 0.1% aqueous formic acid and acetonitrile at a flow rate of 0.45 mL/min. The method validation results demonstrated the developed method possessing desirable reproducibility, efficiency, and allowing fingerprint analysis in one chromatographic run within 13 min. The quality assessment was achieved by using chemometrics methods including similarity analysis, hierarchical clustering analysis and principal component analysis. The developed method can be used for further quality control of D. triquetrum.
authors.affiliation: Department of Pharmacy, Tongde Hospital of Zhejiang Province, 234 Gucui Road, Hangzhou 310012, China zml9998@sina.com.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Xianrui
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Pharmacy, Tongde Hospital of Zhejiang Province, 234 Gucui Road, Hangzhou 310012, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ying
authors.affiliation: Department of Pharmacy, Tongde Hospital of Zhejiang Province, 234 Gucui Road, Hangzhou 310012, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Pharmacy, Tongde Hospital of Zhejiang Province, 234 Gucui Road, Hangzhou 310012, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Tongde Hospital of Zhejiang Province, 234 Gucui Road, Hangzhou 310012, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Jiang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D016000:Cluster Analysis; D007887:Fabaceae / Q000737:chemistry*; D057230:Limit of Detection; D025341:Principal Component Analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*