title: Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal.
abstract: Every year, the list of mammalian species for which cultures of pluripotent stem cells (PSCs) are generated increases. PSCs are a unique tool for extending the limits of experimental studies and modeling different biological processes. In this work, induced pluripotent stem cells (iPSCs) from the hybrids of common voles Microtus levis and Microtus arvalis, which are used as model objects to study genome organization on the molecular-genetic level and the mechanisms of X-chromosome inactivation, have been generated. Vole iPSCs were isolated and cultured in a medium containing cytokine LIF, basic fibroblast growth factor (bFGF), ascorbic acid, and fetal bovine serum. Undifferentiated state of vole iPSCs is maintained by activation of their endogenous pluripotency genes - Nanog, Oct4, Sox2, Sall4, and Esrrb. The cells were able to maintain undifferentiated state for at least 28 passages without change in their morphology and give rise to three germ layers (ectoderm, mesoderm and endoderm) upon differentiation.
authors.affiliation: Federal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Grigor'eva
authors.affiliation: Federal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Shevchenko
authors.affiliation: Federal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia ; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Medvedev
authors.affiliation: Federal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurok
authors.affiliation: Federal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Zhelezova
authors.affiliation: Federal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia ; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
authors.forename: S M
authors.initials: SM
authors.lastname: Zakian
keywords: common voles; induced pluripotent stem cells; reprogramming