title: Culture and Identification of Mouse Bone Marrow-Derived Dendritic Cells and Their Capability to Induce T Lymphocyte Proliferation.
abstract: BACKGROUND
  The aim of this study was to establish a culture method for mouse dendritic cells (DCs) in vitro and observe their morphology at different growth stages and their ability to induce the proliferation of T lymphocytes.
  
  
  METHODS
  Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) were used in combination to induce differentiation of mouse bone marrow (BM) mononucleocytes into DCs. The derived DCs were then assessed for morphology, phenotype, and function.
  
  
  RESULTS
  The mouse BM-derived mononucleocytes had altered cell morphology 3 days after induction by GM-CSF and IL-4 and grew into colonies. Typical dendrites appeared 8 days after induction. Many mature DCs were generated, with typical dendritic morphology observed under scanning electron microscopy. Expression levels of CD11c, a specific marker of BM-derived DCs, and of co-stimulatory molecules such as CD40, CD80, CD86, and MHC-II were elevated in the mature DCs. Furthermore, the mature DCs displayed a strong potency in stimulating the proliferation of syngenic or allogenic T lymphocytes.
  
  
  CONCLUSIONS
  Mouse BM-derived mononucleocytes cultured in vitro can produce a large number of DCs, as well as immature DCs, in high purity. The described in vitro culture method lays a foundation for further investigations of anti-tumor vaccines.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).
authors.forename: Wenguang
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China (mainland).
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).
authors.forename: Baihetiya
authors.initials: B
authors.lastname: Azhati
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).
authors.forename: Mulati
authors.initials: M
authors.lastname: Rexiati
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000648:ultrastructure; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology*; D013997:Time Factors