title: Oligodendrocyte precursors migrate along vasculature in the developing nervous system.
abstract: Oligodendrocytes myelinate axons in the central nervous system and develop from oligodendrocyte precursor cells (OPCs) that must first migrate extensively during brain and spinal cord development. We show that OPCs require the vasculature as a physical substrate for migration. We observed that OPCs of the embryonic mouse brain and spinal cord, as well as the human cortex, emerge from progenitor domains and associate with the abluminal endothelial surface of nearby blood vessels. Migrating OPCs crawl along and jump between vessels. OPC migration in vivo was disrupted in mice with defective vascular architecture but was normal in mice lacking pericytes. Thus, physical interactions with the vascular endothelium are required for OPC migration. We identify Wnt-Cxcr4 (chemokine receptor 4) signaling in regulation of OPC-endothelial interactions and propose that this signaling coordinates OPC migration with differentiation.
authors.affiliation: Department of Pediatrics, University of California at San Francisco (UCSF), San Francisco, CA 94158, USA.
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Tsai
authors.affiliation: Department of Pediatrics, University of California at San Francisco (UCSF), San Francisco, CA 94158, USA.
authors.forename: Jianqin
authors.initials: J
authors.lastname: Niu
authors.affiliation: Departments of Pharmacology and Neuroscience, University of California at San Diego (UCSD), San Diego, CA 92093, USA.
authors.forename: Roeben
authors.initials: R
authors.lastname: Munji
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Davalos
authors.affiliation: Division of Hematology, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: Junlei
authors.initials: J
authors.lastname: Chang
authors.affiliation: Division of Hematology, Department of Medicine, Stanford University, Stanford, CA 94305, USA. Department of Urology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA. Howard Hughes Medical Institute (HHMI), Chevy Chase, MD 20815, USA. Duke University School of Medicine, Durham, NC 27710, USA.
authors.forename: Haijing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, University of California at San Francisco (UCSF), San Francisco, CA 94158, USA.
authors.forename: An-Chi
authors.initials: AC
authors.lastname: Tien
authors.affiliation: Division of Hematology, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: Calvin J
authors.initials: CJ
authors.lastname: Kuo
authors.affiliation: Department of Neurology, UCSF, San Francisco, CA 94158, USA.
authors.forename: Jonah R
authors.initials: JR
authors.lastname: Chan
authors.affiliation: Departments of Pharmacology and Neuroscience, University of California at San Diego (UCSD), San Diego, CA 92093, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Daneman
authors.affiliation: Department of Pediatrics, University of California at San Francisco (UCSF), San Francisco, CA 94158, USA. Department of Neurology, UCSF, San Francisco, CA 94158, USA. Division of Neonatology, UCSF, San Francisco, CA 94158, USA. Newborn Brain Research Institute, UCSF, San Francisco, CA 94158, USA. stephen.fancy@ucsf.edu.
authors.forename: Stephen P J
authors.initials: SP
authors.lastname: Fancy
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000196:embryology; D002465:Cell Movement; D002540:Cerebral Cortex / Q000098:blood supply / Q000196:embryology*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D055495:Neurogenesis; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*; D038081:Organogenesis; D020286:Pericytes / Q000166:cytology / Q000502:physiology; D019718:Receptors, CXCR4 / Q000378:metabolism; D015398:Signal Transduction; D013116:Spinal Cord / Q000098:blood supply / Q000166:cytology / Q000196:embryology*; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: C514936:CXCR4 protein, mouse; D019718:Receptors, CXCR4; D051153:Wnt Proteins