title: Cinnamon oil: A possible alternative for contact lens disinfection.
abstract: OBJECTIVE
  To investigate the antibacterial activity of cinnamon oil alone and in combination with a multipurpose contact lens disinfectant solution (MPS) as well as tobramycin against multi drμg resistant conjunctival bacteria both in planktonic and sessile forms.
  
  
  METHODS
  Minimum inhibitory concentrations (MIC) of tobramycin and cinnamon oil against 19 bacterial strains were investigated against planktonic and sessile cells by micro-dilution methods. Synergistic effects were determined by well diffusion and micro-dilution tissue culture plate methods for planktonic and sessile cells respectively. Time kill assay was performed to study the bactericidal effect of cinnamon oil in concentrations ranging from 5% to 0.312% combined with an MPS with respect to time.
  
  
  RESULTS
  MICs of cinnamon oil against planktonic bacteria ranged from 0.04% to 1.25% versus 0.156% to 5% for sessile cells. Combination of cinnamon oil with tobramycin had a synergistic effect against most tested organisms. The MIC values of cinnamon oil in combination with tobramycin was significantly lower than cinnamon oil alone against biofilm production (P=0.004). Time kill assay revealed that combination of cinnamon oil and disinfectant successfully eradicated the tested microorganisms at all tested concentrations within 2h contact time except for 0.312% concentration (3h) versus 24h for MPS alone.
  
  
  CONCLUSIONS
  Cinnamon oil has a promising antimicrobial effect. It could be a probable candidate for contact lens disinfection.
authors.affiliation: Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Egypt. Electronic address: rhb00@fayoum.edu.eg.
authors.forename: Rasha H
authors.initials: RH
authors.lastname: Bassyouni
authors.affiliation: Botany Department, Faculty of Science, Cairo University, Egypt.
authors.forename: Zeinat
authors.initials: Z
authors.lastname: Kamel
authors.affiliation: Microbiology Department, Research Institute of Ophthalmology, Cairo, Egypt.
authors.forename: Maha Mohssen
authors.initials: MM
authors.lastname: Abdelfattah
authors.affiliation: Botany Department, Faculty of Science, Cairo University, Egypt.
authors.forename: Eman
authors.initials: E
authors.lastname: Mostafa
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002470:Cell Survival / Q000187:drug effects; D018639:Contact Lens Solutions; D003261:Contact Lenses / Q000382:microbiology*; D004203:Disinfection / Q000379:methods; D004338:Drug Combinations; D004866:Equipment Contamination / Q000517:prevention & control*; D005240:Feasibility Studies; D009822:Oils, Volatile / Q000008:administration & dosage*; D014031:Tobramycin / Q000008:administration & dosage*
chemical_list: D000900:Anti-Bacterial Agents; D018639:Contact Lens Solutions; D004338:Drug Combinations; D009822:Oils, Volatile; C510046:cinnamon oil, bark; D014031:Tobramycin
keywords: Antimicrobial; Cinnamon oil; Conjunctival microbiota; Contact lens; Disinfectant