title: Endoglin (CD105) coordinates the process of endometrial receptivity for embryo implantation.
abstract: Endoglin is a TGF-β receptor that is expressed in uterine endothelial and stromal cells in addition to trophoblast expression. However, the functional importance of endoglin in the embryo implantation process is not clear. We observed endoglin expression in the endometrium throughout the stages of its receptivity; however, its expression was enhanced during the receptive stage. Endoglin expression was predominant in epithelial cells of the lumen and glands, but showed a milder expression in stromal cells. Endoglin expression was initially observed in the primary decidual zone and later extended to the secondary decidua zone. Knockdown of endoglin via siRNA reduced the implantation sites along with the blastocyst numbers. Mouse blastocyst with endoglin-silenced endometrial epithelial cells (human and mouse origin) showed poor trophoblast outgrowth, which suggests an essential role for endoglin during endometrial receptivity. In conclusion, our findings reveal the association of endoglin with endometrial receptivity, which is important for embryo attachment.
authors.affiliation: Female Reproductive Biology Laboratory, Division of Endocrinology, CSIR-Central Drug Research Institute, B.S. 10/1, Sector-10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India.
authors.forename: Sangappa Basanna
authors.initials: SB
authors.lastname: Chadchan
authors.affiliation: Female Reproductive Biology Laboratory, Division of Endocrinology, CSIR-Central Drug Research Institute, B.S. 10/1, Sector-10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Female Reproductive Biology Laboratory, Division of Endocrinology, CSIR-Central Drug Research Institute, B.S. 10/1, Sector-10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India.
authors.forename: Vineet Kumar
authors.initials: VK
authors.lastname: Maurya
authors.affiliation: Female Reproductive Biology Laboratory, Division of Endocrinology, CSIR-Central Drug Research Institute, B.S. 10/1, Sector-10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India.
authors.forename: Upendra Kumar
authors.initials: UK
authors.lastname: Soni
authors.affiliation: Female Reproductive Biology Laboratory, Division of Endocrinology, CSIR-Central Drug Research Institute, B.S. 10/1, Sector-10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India. Electronic address: rajesh_jha@cdri.res.in.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Jha
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002478:Cells, Cultured; D003656:Decidua / Q000378:metabolism*; D010064:Embryo Implantation; D000071063:Endoglin / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D014327:Trophoblasts / Q000502:physiology
chemical_list: D000071063:Endoglin; C502004:Eng protein, mouse
keywords: Blastocyst attachment; Decidualization; Delayed implantation; Endoglin; Endometrium receptivity; Implantation sites