title: Attenuation of adhesion, quorum sensing and biofilm mediated virulence of carbapenem resistant Escherichia coli by selected natural plant products.
abstract: The multi-drug resistance offered by Carbapenem Resistant Escherichia coli (Family: Enterobacteriaceae; Class: Gammaproteobacteria) against third line antibiotics can be attributed towards its ability to develop biofilm. Such process involves adhesion and quorum-sensing induced colonization leading to biomass development. The present study explored the anti-adhesion, anti-quorum sensing and anti-biofilm potential of 05 pre-standardized potent herbals. Berberis aristata (PTRC-2111-A) exhibited maximum potential in all these activities i.e. 91.3% ± 0.05% (Anti-adhesion), 96.06% ± 0.05% (Anti-Quorum sensing) and 51.3% ± 0.07% (Anti-Biofilm formation) respectively. Camellia sinensis (PTRC-31911-A) showed both anti-adhesion (84.1% ± 0.03%) and anti-quorum sensing (90.0%) potential while Holarrhena antidysenterica (PTRC-8111-A) showed only anti-quorum sensing potential as compared to standards/antibiotics. These findings were in line with the molecular docking analysis of phytoligands against Lux S and Pilin receptors. Furthermore, the pairwise correlation analysis of the tested activities with qualitative, quantitative and bioactivity functional descriptors revealed that an increased content of alkaloid, moderate content of flavonoids and decreased content of tannins supported all the three activities. In addition, nitric oxide and superoxide scavenging activity were found to be correlated with anti-quorum sensing activity. The findings indicated clearly that B. aristata (Family: Berberidaceae) and C. sinensis (Family: Theaceae) were potent herbal leads with significant therapeutic potential which further needs to be explored at pre-clinical level in the future.
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Thakur
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India. Electronic address: ptrcmanuscripts@gmail.com.
authors.forename: Raman
authors.initials: R
authors.lastname: Chawla
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Tanwar
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: Ankita Singh
authors.initials: AS
authors.lastname: Chakotiya
authors.affiliation: Department of Biotechnology, Jamia Hamdard, Delhi, India.
authors.forename: Alka
authors.initials: A
authors.lastname: Narula
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Goel
authors.affiliation: Office of DG (LS), Defence Research and Development Organisation, DRDO Bhawan, Delhi, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000470:Alkaloids / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D005419:Flavonoids / Q000737:chemistry; D008297:Male; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*; D051381:Rats; D018440:beta-Lactam Resistance
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D001688:Biological Products; D015780:Carbapenems; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Bacterial adhesion; Biofilm; Carbapenem resistant; Escherichia coli; Herbal informatics; Quorum sensing