title: Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing.
abstract: The characterization of bacterial community compositions and the change in perfluoroalkyl acids (PFAAs) along a natural river distribution system were explored in the present study. Illumina high-throughput sequencing was used to explore bacterial community diversity and structure in sediment polluted by PFAAs from the Xiaoqing River, the area with concentrated fluorochemical facilities in China. The concentration of PFAAs was in the range of 8.44-465.60 ng/g dry weight (dw) in sediment. Perfluorooctanoic acid (PFOA) was the dominant PFAA in all samples, which accounted for 94.2 % of total PFAAs. High-level PFOA could lead to an obvious increase in relative abundance of Proteobacteria, ε-Proteobacteria, Thiobacillus, and Sulfurimonas and the decrease in relative abundance of other bacteria. Redundancy analysis revealed that PFOA played an important role in the formation of bacterial community, and PFOA at higher concentration could reduce the diversity of bacterial community. When the concentration of PFOA was below 100 ng/g dw in sediment, no significant effect on microbial community structure was observed. Thiobacillus and Sulfurimonas were positively correlated with the concentration of PFOA, suggesting that both genera were resistant to PFOA contamination.
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.|College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. wangty@rcees.ac.cn.
authors.forename: Tieyu
authors.initials: T
authors.lastname: Wang
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.|College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Xiawei
authors.initials: X
authors.lastname: Peng
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Lu
mesh_terms: D002210:Caprylates / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005466:Fluorocarbons / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000633:toxicity
chemical_list: D002210:Caprylates; D004269:DNA, Bacterial; D005466:Fluorocarbons; D014874:Water Pollutants, Chemical; C023036:perfluorooctanoic acid
keywords: Bacterial community structure; Illumina high-throughput sequencing; PFOA; Redundancy analysis; Sulfurimona; Thiobacillus