title: Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245.
abstract: Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245 as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was found optimum. The optimal conditions for biotransformation were pH 8, 1v/v/m airflow and 500rpm stirring speed. In batch mode of operation, 70.6% of 5g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep(®) 900 (OH(-)), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation.
authors.affiliation: Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden; Department of Botany, Faculty of Science, South Valley University, Qena, Egypt.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Sayed
authors.affiliation: Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden; Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Egypt.
authors.forename: Tarek
authors.initials: T
authors.lastname: Dishisha
authors.affiliation: Department of Botany, Faculty of Science, South Valley University, Qena, Egypt.
authors.forename: Waiel F
authors.initials: WF
authors.lastname: Sayed
authors.affiliation: Department of Botany, Faculty of Science, South Valley University, Qena, Egypt.
authors.forename: Wesam M
authors.initials: WM
authors.lastname: Salem
authors.affiliation: Department of Botany, Faculty of Science, South Valley University, Qena, Egypt.
authors.forename: Hanan A
authors.initials: HA
authors.lastname: Temerk
authors.affiliation: Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden; Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea. Electronic address: Sang-Hyun.Pyo@biotek.lu.se.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Pyo
mesh_terms: D000085:Acetates / Q000378:metabolism; D055162:Biocatalysis; D020148:Butyric Acid / Q000737:chemistry / Q000378:metabolism*; D003352:Corynebacterium / Q000254:growth & development* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D010084:Oxidation-Reduction; D011409:Propylene Glycols / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D014994:Xylose / Q000378:metabolism
chemical_list: D000085:Acetates; D011409:Propylene Glycols; C018163:trimethylolpropane; D020148:Butyric Acid; D014994:Xylose; D005947:Glucose
keywords: 2,2-Bis(hydroxymethyl)butyric acid; Corynebacterium sp.; Product recovery; Selective oxidation; Trimethylolpropane; Whole cell biotransformation