title: Role of Baicalin in Anti-Influenza Virus A as a Potent Inducer of IFN-Gamma.
abstract: Baicalin (BA) is a flavonoid compound purified from Scutellaria baicalensis Georgi and has been shown to possess a potent inhibitory activity against viruses. However, the role of BA in anti-influenza virus has not been extensively studied, and the immunological mechanism of BA in antiviral activity remains unknown. Here, we observed that BA could protect mice from infection by influenza virus A/PR/8/34 (H1N1), associated with increasing IFN-γ production, but presented no effects in IFN-γ or IFN-γ receptor deficient mice. Further study indicated that BA could inhibit A/PR/8/34 replication through IFN-γ in human PBMC. Moreover, BA can directly induce IFN-γ production in human CD4(+) and CD8(+) T cells and NK cells, and activate JAK/STAT-1 signaling pathway. Collectively, BA exhibited anti-influenza virus A (H1N1) activity in vitro and in vivo as a potent inducer of IFN-γ in major IFN-γ producing cells.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China; Key Laboratory of Medical Immunology, Ministry of Health, Beijing 100191, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China; Key Laboratory of Medical Immunology, Ministry of Health, Beijing 100191, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Pharmacy Departments, Liaoning University of Traditional Chinese Medicine, Liaoning 116600, China.
authors.forename: Ming-Bo
authors.initials: MB
authors.lastname: Zhang
authors.affiliation: Pharmacy Departments, Liaoning University of Traditional Chinese Medicine, Liaoning 116600, China.
authors.forename: Zheng-Yun
authors.initials: ZY
authors.lastname: Chu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China; Key Laboratory of Medical Immunology, Ministry of Health, Beijing 100191, China.
authors.forename: Yue-Dan
authors.initials: YD
authors.lastname: Wang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D005419:Flavonoids; C038044:baicalin; D007371:Interferon-gamma