title: In vitro anticancer potential of BaCO3 nanoparticles synthesized via green route.
abstract: Green synthesis of nanoparticles is a growing research area because of their potential applications in nanomedicine. Barium carbonate nanoparticles (BaCO3 NPs) were synthesized using an aqueous extract of Mangifera indica seed as a reducing agent. These particles were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM), selected area electron diffraction (SAED), Energy-dispersive-X-ray (EDX) and X-ray photoelectron spectroscopy (XPS) analysis. HR-TEM images are confirmed that green synthesized BaCO3 NPs have spherical, triangular and uneven shapes. EDX analysis confirmed the presence of Ba, C and O. The peaks at 2θ of 19.45, 23.90, 24.29, 27.72, 33.71, 34.08, 34.60, 41.98, 42.95, 44.18, 44.85, and 46.78 corresponding to (110), (111), (021), (002), (200), (112), (130), (221), (041), (202), (132) and (113) showed that BaCO3 NPs average size was ~18.3 nm. SAED pattern confirmed that BaCO3 NPs are crystalline nature. BaCO3 NPs significantly inhibited cervical carcinoma cells, as evidenced by cytotoxicity assay. Immunofluorescence and fluorescence assays showed that BaCO3 NPs increased the expression and activity of caspase-3, an autocatalytic enzyme that promotes apoptosis. According to the results, green synthesis route has great potential for easy, rapid, inexpensive, eco-friendly and efficient development of novel multifunctional nanoparticles for the treatment of cancer.
authors.affiliation: School of Mechanical Engineering, Yeungnam University, 214-1 Dae-dong, Gyeongsan-si, Gyeongsangbuk-do 712-749, Republic of Korea.
authors.forename: P C
authors.initials: PC
authors.lastname: Nagajyothi
authors.affiliation: Dept of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: Muthuraman
authors.initials: M
authors.lastname: Pandurangan
authors.affiliation: School of Chemical Engineering, Yeungnam University, 214-1 Dae-dong, Gyeongsan-si, Gyeongsangbuk-do 712-749, Republic of Korea. Electronic address: tvmsreekanth@gmail.com.
authors.forename: T V M
authors.initials: TV
authors.lastname: Sreekanth
authors.affiliation: School of Mechanical Engineering, Yeungnam University, 214-1 Dae-dong, Gyeongsan-si, Gyeongsangbuk-do 712-749, Republic of Korea. Electronic address: jshim@ynu.ac.kr.
authors.forename: Jaesool
authors.initials: J
authors.lastname: Shim
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001464:Barium / Q000737:chemistry / Q000494:pharmacology*; D002254:Carbonates / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D002254:Carbonates; D001464:Barium; C006685:barium carbonate
keywords: BaCO(3) nanoparticles; Caspase-3; Cytotoxicity; Green synthesis; Mangifera indica