title: MicroRNA-214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3.
abstract: MicroRNAs (miRNAs) are important gene regulators that participate in tumorigenesis. Previous studies have implicated that miR-214 is a tumor suppressor that is capable of inhibiting human hepatocellular carcinoma (HCC) cell growth. However, the mechanism by which miR-214 suppresses tumor development remains unknown. In the present study, miR-214 was observed to suppress tumor proliferation by directly targeting E2F transcription factor 3 (E2F3) in HCC cells. Colony formation, cell cycle and proliferation assays were employed to study the tumor suppressor role of miR-214 in cell proliferation. In addition, western blotting and dual-luciferase reporter assays were used to evaluate whether E2F3 was a target of miR-214. The results of these analyses revealed that E2F3 was a novel target of miR-214. Furthermore, enhanced expression of miR-214 or silencing of E2F3 inhibited the proliferation of HCC SMMC-7721 cells. These findings suggest that miR-214 suppresses HCC growth by targeting E2F3, and may provide a novel approach for the treatment of human HCC.
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Su'e
authors.initials: S
authors.lastname: Chang
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Zhenghao
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: N I
authors.initials: NI
authors.lastname: Hou
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Lumin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Cai
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: B O
authors.initials: BO
authors.lastname: Guo
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Tong
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Tusheng
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China; Cardiovascular Research Center, Xi'an Jiaotong University, College of Medicine, Xi'an, Shaanxi 710061, P.R. China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
keywords: E2F3; hepatocellular carcinoma; miR-214; proliferation