title: Biological characteristics of a novel giant cell tumor cell line derived from spine.
abstract: Giant cell tumor of bone(GCTB) is a special bone tumor for it consists of various cell types, and its biological characteristics is different from common benign or malignant neoplasm. In the present study, we report the biological features of a primary Asian GCTB cell line named GCTB28. We analyzed extensive properties of the GCTB28 cells including morphological observations, growth, cell cycle, karyotype, proliferation, proteins expression, surface biomarker verification, and tumorigenicity in nude mice. We found that the stromal cells of GCTB were endowed with self-renewal capacity and played dominant roles in GCTB development. Moreover, we confirmed that GCTB cells can be CD33(-)CD14(-) phenotype which was not in accord with previous study. This study provides an in vitro model system to investigate pathogenic mechanisms and molecular characteristics of GCTB and also provides a useful tool for researching the therapeutic targeting of GCTB.
authors.affiliation: Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China.|Department of Orthopaedics, The 169th Hospital of PLA, Hengyang, Hunan, China.|Department of Orthopaedics, Xiangnan Hospital, College of Medicine, Hunan Normal University, Hengyang, Hunan, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China.|Xiamen Hospital of Traditional Chinese Medicine, Xiamen, China.
authors.forename: Leqin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China.
authors.forename: Su
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China. jianruxiao63@126.com.
authors.forename: Jianru
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018212:Giant Cell Tumor of Bone / Q000235:genetics / Q000473:pathology*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008297:Male; D051379:Mice; D008819:Mice, Nude; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000235:genetics; D013131:Spine / Q000473:pathology*; D017154:Stromal Cells / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D018950:Lipopolysaccharide Receptors; D063268:Sialic Acid Binding Ig-like Lectin 3
keywords: Biomarker; Giant cell tumor of bone (GCTB); Mononuclear stromal cells; Multinuclear giant cells; Novel cell line of GCTB