title: Validation of a mass spectrometry-based method for milk traces detection in baked food.
abstract: A simple validated LC-MS/MS-based method was set up to detect milk contamination in bakery products, taking the effects of food processing into account for the evaluation of allergen recovery and quantification. Incurred cookies were prepared at eight levels of milk contamination and were cooked to expose all milk components, including allergenic proteins, to food processing conditions. Remarkable results were obtained in term of sufficiently low LOD and LOQ (1.3 and 4 mg/kg cookies, respectively). Precision was calculated as intra-day repeatability (RSD in the 5-20% range) and inter-day repeatability (4 days; RSD never exceeded 12%). The extraction recovery values ranged from 20% to 26%. Method applicability was evaluated by analysing commercial cookies labelled either as "milk-free" or "may contain milk". Although the ELISA methodology is considered the gold standard for detecting allergens in foods, this robust LC-MS/MS approach should be a useful confirmatory method for assessing and certifying "milk-free" food products.
authors.forename: Cristina
authors.initials: C
authors.lastname: Lamberti
authors.affiliation: ISPA-CNR, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy. Electronic address: cristina.lamberti@ispa.cnr.it.
authors.forename: Lamberti
authors.initials: L
authors.lastname: Cristina
authors.forename: Elena
authors.initials: E
authors.lastname: Acquadro
authors.affiliation: ABLE BioSciences, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy. Electronic address: acquadro@bipcamail.it.
authors.forename: Acquadro
authors.initials: A
authors.lastname: Elena
authors.forename: Davide
authors.initials: D
authors.lastname: Corpillo
authors.affiliation: ABLE BioSciences, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy. Electronic address: corpillo@bioindustrypark.it.
authors.forename: Corpillo
authors.initials: C
authors.lastname: Davide
authors.forename: Marzia
authors.initials: M
authors.lastname: Giribaldi
authors.affiliation: ISPA-CNR, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy. Electronic address: marzia.giribaldi@ispa.cnr.it.
authors.forename: Giribaldi
authors.initials: G
authors.lastname: Marzia
authors.forename: Lucia
authors.initials: L
authors.lastname: Decastelli
authors.affiliation: Centro Regionale Allergie e Intolleranze Alimentari, SS Controllo Alimenti, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy. Electronic address: Lucia.Decastelli@izsto.it.
authors.forename: Decastelli
authors.initials: D
authors.lastname: Lucia
authors.forename: Cristiano
authors.initials: C
authors.lastname: Garino
authors.affiliation: Dipartimento di Scienze del farmaco & DFB Center, Università del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100 Novara, Italy. Electronic address: cristiano.garino@pharm.unipmn.it.
authors.forename: Garino
authors.initials: G
authors.lastname: Cristiano
authors.forename: Marco
authors.initials: M
authors.lastname: Arlorio
authors.affiliation: Dipartimento di Scienze del farmaco & DFB Center, Università del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100 Novara, Italy. Electronic address: marco.arlorio@pharm.unipmn.it.
authors.forename: Arlorio
authors.initials: A
authors.lastname: Marco
authors.forename: Carlo
authors.initials: C
authors.lastname: Ricciardi
authors.affiliation: Politecnico di Torino, DISAT - Applied Science and Technology Dep., Corso Duca degli Abruzzi 24, 10129 Turin, Italy. Electronic address: carlo.ricciardi@polito.it.
authors.forename: Ricciardi
authors.initials: R
authors.lastname: Carlo
authors.forename: Laura
authors.initials: L
authors.lastname: Cavallarin
authors.affiliation: ISPA-CNR, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy. Electronic address: laura.cavallarin@ispa.cnr.it.
authors.forename: Cavallarin
authors.initials: C
authors.lastname: Laura
authors.affiliation: ISPA-CNR, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy. Electronic address: gabriella.giuffrida@ispa.cnr.it.
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Giuffrida
authors.forename: Giuffrida Maria
authors.initials: GM
authors.lastname: Gabriella
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Bakery products; Food processing; Hidden allergens; LC–MS/MS; Milk-free labelling