title: Complete genome sequence of cyanobacterium Fischerella sp. NIES-3754, providing thermoresistant optogenetic tools.
abstract: Cyanobacterial phytochrome-class photosensors are recently emerging optogenetic tools. We isolated Fischerella sp. strain NIES-3754 from hotspring at Suwa-shrine, Suwa, Nagano, Japan. We determined complete genome sequence of the NIES-3754 strain, which is composed of one chromosome and two putative replicons (total 5,826,863bp containing no gaps). We identified photosensor genes of 5 phytochromes and 9 cyanobacteriochromes, which will facilitate optogenetics of thermophile.
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan; Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan. Electronic address: hirose@ens.tut.ac.jp.
authors.forename: Yuu
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Center for Information Biology, National Institute of Genetics, Research Organization of Information and Systems, Yata, Mishima 411-8540, Japan.
authors.forename: Takatomo
authors.initials: T
authors.lastname: Fujisawa
authors.affiliation: College of Industrial Technology, Nihon University, 2-11-1 Shinei, Narashino, Chiba 275-8576, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ohtsubo
authors.affiliation: Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, Miyagi 980-8577, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Katayama
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Misawa
authors.affiliation: Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Wakazuki
authors.affiliation: Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimura
authors.affiliation: Center for Information Biology, National Institute of Genetics, Research Organization of Information and Systems, Yata, Mishima 411-8540, Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kawachi
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Eki
authors.affiliation: Genome Research Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: y3kanesa@nodai.ac.jp.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016680:Genome, Bacterial; D045482:Hot Springs; D006358:Hot Temperature; D007564:Japan; D008969:Molecular Sequence Data; D062308:Optogenetics; D020283:Photoreceptors, Microbial / Q000235:genetics; D010834:Phytochrome / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D012335:RNA, Ribosomal; D010834:Phytochrome
keywords: Cyanobacteria; Genome; Photosensor; Thermophile