title: Blockage of K(Ca)3.1 and Kv1.3 channels of the B lymphocyte decreases the inflammatory monocyte chemotaxis.
abstract: OBJECTIVE
  To investigate the effects of Ca(2+) activated potassium channel KCa3.1 and voltage-gated potassium channel Kv1.3 of B lymphocyte on inflammatory monocytes chemotaxis and the potential mechanisms.
  
  
  METHODS
  Thanswell test was used to detect the inflammatory monocyte (Ly-6C(hi)) chemotaxis caused by the B lymphocyte. Enzyme-linked immunosorbent assay (ELISA) was applied to detecting the C-C motif ligand 7 (CCL7) in cultured media. Cell counting kit-8 (CCK) was used to detect the proliferation of B lymphocytes after activation and blockage of both KCa3.1 and Kv1.3 channels. Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase (P-ERK) of the B lymphocytes.
  
  
  RESULTS
  When activated, B lymphocytes significantly proliferated. After application of KCa3.1 channel-specific inhibitor TRAM-34 and potent Kv1.3 channel inhibitor ShK, both B lymphocytes proliferation and Ly-6C(hi) monocyte chemotaxis were significantly inhibited. The expression of chemotaxis related factor CCL7 decreased remarkably.
  
  
  CONCLUSIONS
  The opening of KCa3.1 and Kv1.3 channels promote B lymphocyte activation, proliferation and Ly-6C(hi) monocyte chemotaxis. The increase of CCL7 secretion by B lymphocyte may explain the pro migration effects.
authors.affiliation: Department of Cardiology, Puyang Oilfield General Hospital, 124 Daqing Road, Puyang 457400, China. Electronic address: shuangxiazhang@yahoo.com.
authors.forename: Shuangxia
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Zhengzhou University People's Hospital, 7 Weiwu Road, 450003 Zhengzhou, China. Electronic address: xianpei_wang@163.com.
authors.forename: Xianpei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Zhengzhou University People's Hospital, 7 Weiwu Road, 450003 Zhengzhou, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Ju
authors.affiliation: Department of Cardiology, Zhengzhou University People's Hospital, 7 Weiwu Road, 450003 Zhengzhou, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Research Center of Ion Channelopathy, Institute of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Cardiology, Zhengzhou University People's Hospital, 7 Weiwu Road, 450003 Zhengzhou, China. Electronic address: gaocy6802@163.com.
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Gao
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D054410:Chemokine CCL7 / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology*; D003064:Cnidarian Venoms / Q000494:pharmacology*; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D051664:Kv1.3 Potassium Channel / Q000037:antagonists & inhibitors / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D011720:Pyrazoles / Q000494:pharmacology*
chemical_list: C515451:Ccl7 protein, mouse; D054410:Chemokine CCL7; D003064:Cnidarian Venoms; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496925:Kcnn4 protein, mouse; D051664:Kv1.3 Potassium Channel; D011720:Pyrazoles; C095391:ShK neurotoxin; C411671:TRAM 34; D002118:Calcium
keywords: B lymphocyte; K(Ca)3.1 channel; Kv1.3 channel; Ly-6C(hi) monocyte