title: Cholesterol Transporters ABCA1 and ABCG1 Gene Expression in Peripheral Blood Mononuclear Cells in Patients with Metabolic Syndrome.
abstract: ABCA1 and ABCG1 genes encode the cholesterol transporter proteins that play a key role in cholesterol and phospholipids homeostasis. This study was aimed at evaluating and comparing ABCA1 and ABCG1 genes expression in metabolic syndrome patients and healthy individuals. This case-control study was performed on 36 patients with metabolic syndrome and the same number of healthy individuals in Hamadan (west of Iran) during 2013-2014. Total RNA was extracted from mononuclear cells and purified using RNeasy Mini Kit column. The expression of ABCA1 and ABCG1 genes was performed by qRT-PCR. Lipid profile and fasting blood glucose were measured using colorimetric procedures. ABCG1 expression in metabolic syndrome patients was significantly lower (about 75%) compared to that of control group, while for ABCA1 expression, there was no significant difference between the two studied groups. Comparison of other parameters such as HDL-C, FBS, BMI, waist circumference, and systolic and diastolic blood pressure between metabolic syndrome patients and healthy individuals showed significant differences (P < 0.05). Decrease in ABCG1 expression in metabolic syndrome patients compared to healthy individuals suggests that hyperglycemia, related metabolites, and hyperlipidemia over the transporter capacity resulted in decreased expression of ABCG1. Absence of a significant change in ABCA1 gene expression between two groups can indicate a different regulation mechanism for ABCA1 expression.
authors.affiliation: Department of Clinical Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tavoosi
authors.affiliation: Department of Biochemistry, Medical School, Tehran University of Medical Sciences, Tehran 14176 13151, Iran.
authors.forename: Hemen
authors.initials: H
authors.lastname: Moradi-Sardareh
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran; Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Yadegarazari
authors.affiliation: Department of Internal Medicine, Medical School, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
authors.forename: Shiva
authors.initials: S
authors.lastname: Borzuei
authors.affiliation: Department of Biostatistics & Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Soltanian
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Goodarzi