title: A novel silk fibroin nanofibrous membrane for guided bone regeneration: a study in rat calvarial defects.
abstract: A novel membrane for guided bone regeneration (GBR), constituting silk fibroin (SF) nanofiber from native silk nanofibril solution, was prepared by electrospinning process. Another barrier membrane, a collagen-type membrane (Bio-Gide®), was used as a comparative sample. Twelve healthy male Sprague-Dawley rats were used in this study. Bilateral round defects were created in the calvarial bone. The bone regenerative efficacy was evaluated in rat calvarial defects. Animals were killed at 4 and 12 weeks. Bone regeneration was analyzed using micro-computed tomography and histological analysis. The SF nanofibrous membrane showed superior results with regard to mechanical tensile properties. At 4 weeks, the bone volume and collagen I positive areas in the SF group were greater than in the Bio-Gide group. At 12 weeks, the defect had completely healed with new bone in both the groups. In conclusion, the SF nanofibrous membranes showed satisfactory mechanical stability, good biocompatibility, slow degradability, and improved new bone regeneration without any adverse inflammatory reactions. Considering the low cost and low risk of disease transmission, the SF nanofibrous membrane is a potential candidate for GBR therapy compared with the widely used collagen membranes.
authors.affiliation: Institute of Stomatology, Chinese People Liberation Army General HospitalBeijing, China; Department of Stomatology, Suzhou Health CollegeSuzhou, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Lu
authors.affiliation: National Engineering Laboratory for Modern Silk, College Of Textile And Clothing Engineering, Soochow University Suzhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory of Stem Cell Research, Medical College, Soochow University Suzhou 215123, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital Of Soochow University Suzhou, China.
authors.forename: Xichao
authors.initials: X
authors.lastname: Zhou
authors.affiliation: National Engineering Laboratory for Modern Silk, College Of Textile And Clothing Engineering, Soochow University Suzhou, China.
authors.forename: Baoqi
authors.initials: B
authors.lastname: Zuo
authors.affiliation: Jiangsu Province Key Laboratory of Stem Cell Research, Medical College, Soochow University Suzhou 215123, China.
authors.forename: Xinran
authors.initials: X
authors.lastname: You
authors.affiliation: Department of Stomatology, The First Affiliated Hospital Of Soochow University Suzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Stomatology, Chinese People Liberation Army General Hospital Beijing, China.
authors.forename: Hongchen
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University Shanghai, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Tang
keywords: Silk fibroin nanofiber; guided bone regeneration (GBR); rat calvarial defects