title: Modified pectic polysaccharide from turmeric (Curcuma longa): A potent dietary component against gastric ulcer.
abstract: Native, intact (TrPP) and modified, low-molecular-weight (MTrPP) forms of pectic polysaccharides isolated from turmeric were evaluated for ulcer-preventive potentials in in vitro and in vivo models. Data indicated that MTrPP possessed significantly better ulcer-preventive property than TrPP; inhibiting ulcer scores up to 85%. Results were substantiated by effective muco-protection, H(+),K(+)-ATPase down-regulation, inhibition of H. pylori growth/adherence, higher antioxidant/cytoprotective mechanisms. Structural data indicated TrPP and MTrPP differ in their molecular weights and structural characteristics with different sugar compositions and side chain ratios. MTrPP was rich in galacturonic acid (687mg/g; TrPP-544mg/g) and galactose (52.9%; TrPP-21.7%). Results were substantiated by NMR/FTIR data indicating the presence of homogalacturonan and rhamnogalacturonam-I containing galactans. By virtue of binding to inflammatory marker (galectin-3), galactans may reduce inflammation induced ulcerations. The low molecular weight of MTrPP (155kDa; TrPP-13kDa) may increase its bioavailability than TrPP, thus MTrPP may possess higher antiulcer potential.
authors.affiliation: Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, KRS Road, Mysore, Karnataka, India.
authors.forename: Mysore R
authors.initials: MR
authors.lastname: Harsha
authors.affiliation: Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, KRS Road, Mysore, Karnataka, India.
authors.forename: Serkad V
authors.initials: SV
authors.lastname: Chandra Prakash
authors.affiliation: Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, KRS Road, Mysore, Karnataka, India. Electronic address: shylaja@cftri.res.in.
authors.forename: Shylaja M
authors.initials: SM
authors.lastname: Dharmesh
mesh_terms: D000897:Anti-Ulcer Agents / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D002460:Cell Line; D030024:Curcuma / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005690:Galactose / Q000032:analysis; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects; D006603:Hexuronic Acids / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010368:Pectins / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared; D013276:Stomach Ulcer / Q000188:drug therapy / Q000517:prevention & control
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D006603:Hexuronic Acids; D011134:Polysaccharides; C042491:rhamnogalacturonan I; C007819:galacturonic acid; D010368:Pectins; D017506:H(+)-K(+)-Exchanging ATPase; C003181:polygalacturonic acid; D005690:Galactose
keywords: Antiulcer; Dietary/natural; Galactan; Modified pectin; Turmeric