title: Comparison of in vitro antibacterial activities of two cationic peptides CM15 and CM11 against five pathogenic bacteria: Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio cholerae, Acinetobacter baumannii, and Escherichia coli.
abstract: In recent years, the widespread use of antibiotics has caused many bacterial pathogens resistance to conventional antibiotics. Therefore, generation of new antibiotics to control and reduce the effects of these pathogens is urgently needed. Antimicrobial peptides and proteins are important members of the host defense system in eukaryotes. These peptides are potent, broad-spectrum antibiotics that demonstrate potential as novel and alternative therapeutic agents for the treatment of drug-resistant infections. Accordingly, we evaluated two hybrid peptides CM11 (WKLFKKILKVL-NH2) and CM15 (KWKLFKKIGAVLKVL-NH2) on five important pathogenic bacteria. These peptides are short cecropin-melittin hybrid peptides obtained through a sequence combination approach, which are highly effective to inhibit the growth of important pathogenic bacteria. The activity of these two cationic peptides (CM11 and CM15) in different concentrations (2-64 mg/L) was investigated against standard and clinical isolates of important hospital infection bacteria by measuring MIC, MBC, and bactericidal assay. These peptides demonstrated the same ranges of inhibitory values: The organisms in early 24 h were more susceptible to polycationic peptides (MIC: 8 mg/L and MBC 32 mg/L), but after 48 h the MIC and MBC remained constant for the CM11 peptide. Bactericidal assay showed that all bacteria strains did not have any growth in agar plates after 40 min. The result showed that these two peptides are more effective than other peptides.
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Vanak Sq. Mollasadra St., P.O. Box: 19395-5487, Tehran, Iran. mm.genetics@gmail.com.
authors.forename: M Moosazadeh
authors.initials: MM
authors.lastname: Moghaddam
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Vanak Sq. Mollasadra St., P.O. Box: 19395-5487, Tehran, Iran. Abolhassani@gmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Abolhassani
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Vanak Sq. Mollasadra St., P.O. Box: 19395-5487, Tehran, Iran. hbabavalian@yahoo.com.
authors.forename: H
authors.initials: H
authors.lastname: Babavalian
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. rmirnejadreza@yahoo.com.
authors.forename: R
authors.initials: R
authors.lastname: Mirnejad
authors.affiliation: Department of Molecular Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran. azizi64@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Azizi Barjini
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Vanak Sq. Mollasadra St., P.O. Box: 19395-5487, Tehran, Iran. Jafar.amani@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Amani
keywords: Antimicrobial peptides; Cecropin; Hybrid peptide; Melittin