title: A HPLC-MS/MS method for determination of 6'''-feruloylspinosin in rat plasma and tissues: Pharmacokinetics and tissue distribution study.
abstract: A sensitive, reliable and accurate HPLC-MS/MS method was developed and validated for the quantification of 6'''-feruloylspinosin in rat plasma and tissues with puerarin as the internal standard. The separation was performed on a Proshell 120 EC-C18 column (4.6×150 mm, 2.7 μm) with a mobile phase consisting of acetonitrile and 0.1% formic acid (20:80, v/v) at 0.3 mL/min. The quantification was performed by MRM with m/z [M-H](-) 783.3→427.2 for 6'''-feruloylspinosin and m/z [M-H](-) 415.4→295.4 for the internal standard, respectively. The calibration curves covered over a concentration range of 20-2000 ng/mL in plasma and various tissues samples (heart, liver, spleen, lung, kidney, stomach, intestine, muscle, cerebrum and cerebellum) with good linearity (r(2)≥0.9914). Both the intra- and inter-day precisions were less than 14.70%, and the accuracy (RE%) ranged from -5.80% to 4.93%. The extraction recoveries were within 75.21-92.96%, and the matrix effect ranged from 87.21% to 113.44%. Compared with spinosin, 6'''-feruloylspinosin was distributed in rats faster whereas more slowly eliminated from the plasma. 6'''-Feruloylspinosin could be distributed rapidly and widely in various tissues, and transfer across the blood-brain barrier. In addition, both 6'''-feruloylspinosin and spinosin could enhance the expression of GABAAα1, GABAAα5, GABABR1 mRNA in rat hippocampal neurons significantly, indicating the bioactivity mechanism of 6'''-feruloylspinosin was involved in the GABA receptors.
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China.
authors.forename: Longdong
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Department of pharmacy, Weifang People's Hospital, Weifang 261041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266100, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China. Electronic address: xjbo@tjcu.edu.cn.
authors.forename: Junbo
authors.initials: J
authors.lastname: Xie
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China. Electronic address: zhyqing@tjcu.edu.cn.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Duan
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Xie
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics*; D006624:Hippocampus / Q000378:metabolism; D007529:Isoflavones / Q000493:pharmacokinetics; D008297:Male; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution / Q000502:physiology*
chemical_list: D005419:Flavonoids; D007529:Isoflavones; C055669:spinosin; C033607:puerarin
keywords: 6′′′-Feruloylspinosin; HPLC-MS/MS; Pharmacokinetics; Spinosin; Tissue distribution