title: Inhibitory effects of antibiofilm compound 1 against Staphylococcus aureus biofilms.
abstract: A novel benzimidazole molecule that was identified in a small-molecule screen and is known as antibiofilm compound 1 (ABC-1) has been found to prevent bacterial biofilm formation by multiple bacterial pathogens, including Staphylococcus aureus, without affecting bacterial growth. Here, the biofilm inhibiting ability of 156 μM ABC-1 was tested in various biofilm-forming strains of S. aureus. It was demonstrated that ABC-1 inhibits biofilm formation by these strains at micromolar concentrations regardless of the strains' dependence on Polysaccharide Intercellular Adhesin (PIA), cell wall-associated protein dependent or cell wall- associated extracellular DNA (eDNA). Of note, ABC-1 treatment primarily inhibited Protein A (SpA) expression in all strains tested. spa gene disruption showed decreased biofilm formation; however, the mutants still produced more biofilm than ABC-1 treated strains, implying that ABC-1 affects not only SpA but also other factors. Indeed, ABC-1 also attenuated the accumulation of PIA and eDNA on cell surface. Our results suggest that ABC-1 has pleotropic effects on several biofilm components and thus inhibits biofilm formation by S. aureus.
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.
authors.forename: Looniva
authors.initials: L
authors.lastname: Shrestha
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.|Project Research Center for Nosocomial Infectious Disease, Hiroshima University.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Kayama
authors.affiliation: Department of Synthetic Organic Chemistry, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku Hiroshima 734-8551, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.|Project Research Center for Nosocomial Infectious Disease, Hiroshima University.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kato
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.|Project Research Center for Nosocomial Infectious Disease, Hiroshima University.
authors.forename: Junzo
authors.initials: J
authors.lastname: Hisatsune
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.
authors.forename: Keiko
authors.initials: K
authors.lastname: Tsuruda
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.|Project Research Center for Nosocomial Infectious Disease, Hiroshima University.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Koizumi
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tatsukawa
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Synthetic Organic Chemistry, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku Hiroshima 734-8551, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences.|Project Research Center for Nosocomial Infectious Disease, Hiroshima University.
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001562:Benzimidazoles / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002473:Cell Wall / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics; D015536:Down-Regulation; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001562:Benzimidazoles; D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger; D013205:Staphylococcal Protein A; C113579:polysaccharide intercellular adhesin; C119204:streptococcal protective antigen; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
keywords: Staphylococcus aureus; antibiofilm compound 1; biofilm components