title: Circulating miR-21, miR-378, and miR-940 increase in response to an acute exhaustive exercise in chronic heart failure patients.
abstract: Congestive heart failure (CHF) is a major cause of hospitalizations, morbidity, and mortality in Western societies. In addition to optimal medical and device therapy, exercise training is an important adjunct treatment option for CHF patients. MicroRNAs (miRNAs, miRs) participate in a variety of physiological and pathological processes. Dynamic regulation of circulating miRNAs during exercise in healthy persons and athletes has recently been documented, however, the response of circulating miRNAs to exercise in CHF patients is undetermined. Twenty-eight CHF patients underwent a symptom-limited incremental cardiopulmonary exercise test on a bicycle ergometer using a standardized exercise protocol of revised Ramp10 programs at Shanghai Tongji Hospital. Blood samples were collected before and immediately after an acute exercise session. RNA was extracted from the serum and selected miRNAs were determined using quantitative polymerase chain reactions. Moreover, inflammatory and muscle damage markers were determined by enzyme linked immunosorbent assays. We found that serum miR-21, miR-378 and miR-940 levels were significantly up-regulated immediately following an acute exercise while the rest were not changed. In addition, no robust correlation was identified between changes of these miRNAs and exercise capacity, muscle damage or inflammation. In conclusion, serum miR-21, miR-378, and miR-940 increase in response to an acute exhaustive exercise in CHF patients. Further studies are needed to clarify the potential use of circulating miRNAs as biomarkers of exercise adaptation in CHF patients, and if they have any use as prognostic markers of cardiovascular outcomes.
authors.affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai 200444, China.|Shanghai Key Laboratory of Bio-Energy Crops, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Tianzhao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai 200444, China.|Shanghai Key Laboratory of Bio-Energy Crops, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Qiulian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Che
authors.affiliation: Cardiovascular Division of the Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Saumya
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Lemin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Jinfa
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Guanghe
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Hongbao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai 200444, China.|Shanghai Key Laboratory of Bio-Energy Crops, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000222:Adaptation, Physiological; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D015444:Exercise / Q000502:physiology; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: exercise; heart failure; microRNA