title: Polysaccharide with antioxidant, α-amylase inhibitory and ACE inhibitory activities from Momordica charantia.
abstract: Functional polysaccharide was isolated from Momordica charantia, with a yield of 36% (w/w). M. charantia bioactive polysaccharide (MCBP) was an acidic and branched heteropolysaccharide with a molecular weight of 92 kDa. Fourier transform infrared spectroscopic analysis indicated that MCBP was a pectin-like polysaccharide with an esterification degree of 53% and it contains numerous monosaccharides, predominantly glucose, galactose, and galaturonic acid. The results also showed that MCBP exhibited free radical scavenging activity (31.9%), ferric reducing antioxidant power (0.95 mM), α-amylase inhibition (89.1%), and angiotensin-converting enzyme inhibition (94.1%). In the terms of functionality, MCBP showed a lower water-holding capacity but higher in oil-holding capacity, emulsifying activity and foaming capacity compared to citrus pectin. Scanning electron microscopy images demonstrated that MCBP formed gels with a porous structure, and flow analysis showed that the gel solution exhibited pseudoplastic shear-thinning behavior. These findings indicated that MCBP is a promising functional macromolecular carbohydrate for the food and nutraceutical industries.
authors.affiliation: School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
authors.forename: Hwee-Feng
authors.initials: HF
authors.lastname: Tan
authors.affiliation: Analytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia. Electronic address: mattgan81@yahoo.com.
authors.forename: Chee-Yuen
authors.initials: CY
authors.lastname: Gan
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002241:Carbohydrates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D029777:Momordica charantia / Q000737:chemistry*; D009005:Monosaccharides; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014574:Uronic Acids; D014783:Viscosity; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D002241:Carbohydrates; D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D014574:Uronic Acids; D000516:alpha-Amylases
keywords: Enzyme inhibitory; Functional polysaccharide; Momordica charantia