title: Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice.
abstract: Neonatal studies in different mouse strains reveal that early life colonization affects the development of adaptive immunity in mice. The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes, but neonatal studies of NOD mice are lacking. We hypothesized that NOD mice deviate from another much used mouse strain, C57BL/6, with respect to postnatal microbiota and/or hematopoiesis and compared this in newborn mice of dams housed under the same conditions. A distinct bacteria profile rich in staphylococci was found at postnatal days (PND) 1-4 in NOD mice. Furthermore, a distinct splenic cell profile high in a granulocytic phenotype was evident in the neonatal NOD mice whereas neonatal C57BL/6 mice showed a profile rich in monocytes. Neonatal expression of Reg3g and Muc2 in the gut was deviating in NOD mice and coincided with fewer bacteria attaching to the Mucosal surface in NOD compared to C57BL/6 mice.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Dina Silke Malling
authors.initials: DS
authors.lastname: Damlund
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Stine Broeng
authors.initials: SB
authors.lastname: Metzdorff
authors.affiliation: Department of Pathology, Rigshospitalet, 2100 Copenhagen, Denmark.
authors.forename: Jane Preuss
authors.initials: JP
authors.lastname: Hasselby
authors.affiliation: Department of Food Science, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Maria
authors.initials: M
authors.lastname: Wiese
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Mia
authors.initials: M
authors.lastname: Lundsager
authors.affiliation: Department of Food Science, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Dennis Sandris
authors.identifier: 0000-0001-8121-1114
authors.initials: DS
authors.lastname: Nielsen
authors.affiliation: Bartholin Institute, Rigshospitalet, 2100 Copenhagen, Denmark.
authors.forename: Karsten Stig
authors.initials: KS
authors.lastname: Buschard
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Axel Kornerup
authors.initials: AK
authors.lastname: Hansen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
authors.forename: Hanne
authors.initials: H
authors.lastname: Frøkiær
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001422:Bacterial Adhesion; D015415:Biomarkers / Q000378:metabolism; D003920:Diabetes Mellitus / Q000097:blood* / Q000235:genetics / Q000382:microbiology*; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome; D018507:Gene Expression Regulation, Developmental; D006098:Granulocytes / Q000378:metabolism; D006410:Hematopoiesis; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D009000:Monocytes / Q000378:metabolism; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D010641:Phenotype; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013210:Staphylococcus / Q000254:growth & development
chemical_list: D015415:Biomarkers; C524626:Muc2 protein, mouse; D055262:Mucin-2; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C490255:Reg3g protein, mouse