title: Layer-by-Layer Assembly of Metal-Organic Frameworks in Macroporous Polymer Monolith and Their Use for Enzyme Immobilization.
abstract: New monolithic materials comprising zeolitic imidazolate framework (ZIF-8) located on the pore surface of poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith previously functionalized with N-(3-aminopropyl)-imidazole have been prepared via a layer-by-layer self-assembly strategy. These new ZIF-8@monolith hybrids are used as solid-phase carriers for enzyme immobilization. Their performance is demonstrated with immobilization of a model proteolytic enzyme trypsin. The best of the conjugates enable very efficient digestion of proteins that can be achieved in mere 43 s.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Liyin
authors.initials: L
authors.lastname: Wen
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Aicong
authors.initials: A
authors.lastname: Gao
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Cao
authors.affiliation: International Research Center for Soft Matter, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D008768:Methylmethacrylates / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis; D016062:Porosity; D020005:Propanols / Q000737:chemistry*; D059748:Proteolysis; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry*
chemical_list: C515394:1-(3-aminopropyl)imidazole; D004800:Enzymes, Immobilized; D007093:Imidazoles; D008768:Methylmethacrylates; D010446:Peptide Fragments; D020005:Propanols; D012710:Serum Albumin, Bovine; C020347:GMA-EDMA; D045304:Cytochromes c; D014357:Trypsin
keywords: enzyme immobilization; layer growth; macroporous polymer monolith; metal-organic framework; self-assembly