title: Transcriptomic profiling of the salt stress response in excised leaves of the halophyte Beta vulgaris ssp. maritima.
abstract: Beta vulgaris ssp. maritima is a halophytic relative of cultivated beets. In the present work a transcriptome response to acute salt stress imposed to excised leaves of sea beet was investigated. Salt treatments consisted of adding NaCl directly to the transpiration stream by immersing the petioles of excised leaves into the salt solutions. Sequencing libraries were generated from leaves subjected to either moderate or strong salt stress. Control libraries were constructed from untreated leaves. Sequencing was performed using the Illumina MiSeq platform. We obtained 32970 unigenes by assembling the pooled reads from all the libraries with Trinity software. Screening the nr database returned 18,362 sequences with functional annotation. Using the reference transcriptome we identified 1,246 genes that were differentially expressed after 48 h of NaCl stress. Genes related to several cellular functions such as membrane transport, osmoprotection, molecular chaperoning, redox metabolism or protein synthesis were differentially expressed in response to salt stress. The response of sea beet leaves to salt treatments was marked out by transcriptomic up-regulation of genes related to photosynthetic carbon fixation, ribosome biogenesis, cell wall-building and cell wall expansion. Furthermore, several novel and undescribed transcripts were responsive to salinity in leaves of sea beet.
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Skorupa
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Gołębiewski
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Domagalski
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kurnik
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Karim
authors.initials: K
authors.lastname: Abu Nahia
authors.affiliation: Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Złoch
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tretyn
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland. Electronic address: tybr@umk.pl.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Tyburski
mesh_terms: D027461:Beta vulgaris / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012965:Sodium Chloride
keywords: Beta vulgaris ssp. maritima; Excised leaves; Leaf transcriptome; Salt stress; Sea beet