title: Highly Flexible Platform for Tuning Surface Properties of Silica Nanoparticles and Monitoring Their Biological Interaction.
abstract: The following work presents a simple, reliable and scalable seeding-growth methodology to prepare silica nanoparticles (SiO2 NPs) (20, 30, 50 and 80 nm) directly in aqueous phase, both as plain- as well as fluorescent-labeled silica. The amount of fluorescent label per particle remained constant regardless of size, which facilitates measurements in terms of number-based concentrations. SiO2 NPs in dispersion were functionalized with an epoxysilane, thus providing a flexible platform for the covalent linkage of wide variety of molecules under mild experimental conditions. This approach was validated with ethylenediamine, two different amino acids and three akylamines to generate a variety of surface modifications. Accurate characterization of particle size, size distributions, morphology and surface chemistry is provided, both for as-synthesized particles and after incubation in cell culture medium. The impact of physicochemical properties of SiO2 NPs was investigated with human alveolar basal epithelial cells (A549) such as the effect in cytotoxicity, cell internalization and membrane interaction.
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Isaac
authors.initials: I
authors.lastname: Ojea-Jiménez
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Patricia
authors.initials: P
authors.lastname: Urbán
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Francisco
authors.initials: F
authors.lastname: Barahona
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Pedroni
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.|Institut de Biologie et Chimie des Protéines, BMSSI-UMR 5086, Université Lyon 1, Université de Lyon , 69367 Lyon, France.
authors.forename: Robin
authors.initials: R
authors.lastname: Capomaccio
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Ceccone
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kinsner-Ovaskainen
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: François
authors.initials: F
authors.lastname: Rossi
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection , Via E. Fermi 2749, 21027 Ispra, Varese, Italy.
authors.forename: Douglas
authors.initials: D
authors.lastname: Gilliland
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry; D004847:Epithelial Cells / Q000187:drug effects*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D003470:Culture Media; D005456:Fluorescent Dyes; D012822:Silicon Dioxide
keywords: A549 cells; cell membrane interaction; epoxide functionalization; fluorescent nanoparticles; silica nanoparticles; surface charge; toxicity; uptake