title: MBT-ASTRA: A suitable tool for fast antibiotic susceptibility testing?
abstract: The increasing resistance to antibiotics is an urgent health care problem. Detection of resistant microorganisms is the pre-requisite for initiating an adequate therapy and implementing respective hygiene measures. Depending on the species and the method employed for analysis, the time to result of antibiotic resistance testing ranges between five and 24h. As MALDI-TOF MS has become an established tool for the fast species identification in microbiological laboratories a time gap between the results of species identification and the information about antibiotic susceptibility arises. Here, we present a semi-quantitative MALDI-TOF MS-based approach for the detection of resistance in different species against different antibiotics.
authors.affiliation: Bruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany. Electronic address: Katrin.Sparbier@bruker.com.
authors.forename: Katrin
authors.initials: K
authors.lastname: Sparbier
authors.affiliation: Max von Pettenkofer-Institute, Institute of Bacteriology, Ludwig Maximilians University Munich, Munich, Germany. Electronic address: Schubert@med.uni-muenchen.de.
authors.forename: Sören
authors.initials: S
authors.lastname: Schubert
authors.affiliation: Bruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany. Electronic address: Markus.Kostrzewa@bruker.com.
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects* / Q000472:pathogenicity; D002439:Cefotaxime / Q000737:chemistry / Q000627:therapeutic use; D002939:Ciprofloxacin / Q000737:chemistry / Q000627:therapeutic use; D004352:Drug Resistance, Microbial; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014031:Tobramycin / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D002439:Cefotaxime; D014031:Tobramycin
keywords: Antibiotic resistance; Antibiotic susceptibility testing; MALDI-TOF MS; Semi-quantitative analysis