title: Preparation of bioactive and antimicrobial PLGA membranes by magainin II/EGF functionalization.
abstract: Development of dual functional materials that are capable of both reducing bacterial interaction and encouraging host tissue integration has gained importance in design of biomaterials. In this study, we prepared a bilayer poly (lactide co-glycolide) fibrous membrane with antibacterial and bioactive properties by electrospinning. The antibacterial layer was produced by covalent immobilization of antimicrobial peptide, Magainin II. The bioactive layer incorporating epidermal growth factor (EGF) molecules was subsequently electrospun on the antibacterial layer. The membranes were characterized by X-ray photoelectron spectroscopy, scanning electron microscopy and fluorescence microscopy. EGF release was detected by enzyme-linked immunosorbent assay. The antibacterial activity was tested against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The ability to support tissue cell integration was detected by using L-929 mouse fibroblasts. The dual functional membranes established enhanced antibacterial properties and increased tissue cell compatibility. This combined approach suggests a promising strategy for wound dressings, vascular grafts and dental membranes as well as catheters and fixation devices.
authors.affiliation: Ankara University, Faculty of Engineering, Chemical Engineering Department, 06100 Ankara, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Yüksel
authors.affiliation: Ankara University, Faculty of Engineering, Chemical Engineering Department, 06100 Ankara, Turkey. Electronic address: akarakecili@eng.ankara.edu.tr.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Karakeçili
authors.affiliation: Hacettepe University, Bioengineering Department, 06800, Beytepe, Ankara, Turkey.
authors.forename: T Tolga
authors.initials: TT
authors.lastname: Demirtaş
authors.affiliation: Hacettepe University, Bioengineering Department, 06800, Beytepe, Ankara, Turkey; Hacettepe University, Chemical Engineering Department 06800, Beytepe, Ankara, Turkey.
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D066255:EGF Family of Proteins / Q000737:chemistry*; D004560:Electricity; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology*; D054806:Magainins / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D066255:EGF Family of Proteins; D054806:Magainins; D008567:Membranes, Artificial; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Antibacterial activity; PLGA membrane; Tissue cell integration