title: Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli.
abstract: Acrylic acid is an important industrial feedstock. In this study, a de novo acrylate biosynthetic pathway from inexpensive carbon source glycerol was constructed in Escherichia coli. The acrylic acid was produced from glycerol via 3-hydroxypropionaldehyde, 3-hydroxypropionyl-CoA, and acrylyl-CoA. The acrylate production was improved by screening and site-directed mutagenesis of key enzyme enoyl-CoA hydratase and chromosomal integration of some exogenous genes. Finally, our recombinant strain produced 37.7 mg/L acrylic acid under shaking flask conditions. Although the acrylate production is low, our study shows feasibility of engineering an acrylate biosynthetic pathway from inexpensive carbon source. Furthermore, the reasons for limited acrylate production and further strain optimization that should be performed in the future were also discussed.
authors.affiliation: CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Tong
authors.affiliation: CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.affiliation: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Niu
authors.affiliation: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Guo
authors.affiliation: CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. liuhuizhou@qibebt.ac.cn.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
authors.affiliation: CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. zhaoguang@qibebt.ac.cn.
authors.forename: Guang
authors.identifier: http://orcid.org/0000-0003-0002-5972
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000179:Acrylates / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000737:chemistry; D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways; D002876:Chromosomes, Bacterial; D003470:Culture Media / Q000737:chemistry; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism*; D007218:Industrial Microbiology; D016297:Mutagenesis, Site-Directed; D011407:Propane / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000179:Acrylates; D000214:Acyl Coenzyme A; D003470:Culture Media; D011994:Recombinant Proteins; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; C047011:acryloyl-coenzyme A; D004746:Enoyl-CoA Hydratase; C036658:acrylic acid; D005990:Glycerol; D011407:Propane
keywords: Acrylic acid biosynthesis; Enoyl-CoA hydratase; Inexpensive carbon source; Recombinant Escherichia coli