title: Antimicrobial activities of ethanol and butanol fractions of white rose petal extract.
abstract: White rose (Rosa hybrida) petals were extracted with ethanol (EtOH) or butanol (BuOH), and tested for their antimicrobial activities against two species of Gram-positive bacteria, six species of Gram-negative bacteria, and two species of fungi. On in vitro antimicrobial assays, Helicobacter pylori and Propionibacterium acnes were highly susceptible to white rose petal extract (WRPE)-EtOH and WRPE-BuOH, leading to minimal inhibitory concentrations of 100 and 10 μg/mL for H. pylori and 400 and 40 μg/mL for P. acnes, respectively. In in vivo experiments, C57BL/6 mice were infected with H. pylori by intragastric inoculation (1 × 10(8) CFU/mouse) 3 times, and orally treated twice a day for 14 days with WRPE-EtOH and WRPE-BuOH. On a CLO kit assay, 200 mg/kg of WRPE-EtOH fully eliminated the bacteria from the gastric mucosa, and the effect of 100 mg/kg of ethanol fraction was similar to pantoprazole (30 mg/kg), displaying 75% elimination. WRPE-BuOH was more effective, exhibiting 75% elimination at 20 mg/kg. The CLO test results were confirmed by bacterial identification. WRPE-EtOH and WRPE-BuOH inhibited the growth of various bacteria and fungi, and in particular, they effectively killed H. pylori and eliminated the bacteria from the mouse stomach. The results indicate that WRPE-EtOH and WRPE-BuOH could be good candidates for the elimination of H. pylori.
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Dongsun
authors.initials: D
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Kyungha
authors.initials: K
authors.lastname: Shin
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Choi
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Cha
authors.affiliation: Department of Biomedical Laboratory Sciences, Semyung University, Jecheon, South Korea.
authors.forename: Sa-Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea.
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
authors.affiliation: Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, South Korea.
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Joo
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Cheongju, South Korea.
authors.forename: Jae Kwon
authors.initials: JK
authors.lastname: Choi
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Cheongju, South Korea.
authors.forename: Yoon Bok
authors.initials: YB
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Ehn-Kyoung
authors.initials: EK
authors.lastname: Choi
authors.affiliation: Department of Biomedical Laboratory Sciences, Yonsei University, Wonju, South Korea.
authors.forename: Jong Bae
authors.initials: JB
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea. Electronic address: solar93@cbu.ac.kr.
authors.forename: Yun-Bae
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000440:Butanols / Q000737:chemistry*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000382:microbiology; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010946:Plants, Medicinal; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000254:growth & development; D027826:Rosa / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000440:Butanols; D012997:Solvents; D000431:Ethanol
keywords: Antimicrobial activity; Butanol fraction; Ethanol fraction; Helicobacter pylori; Propionibacterium acnes; White rose petal extract