title: Functional characterization of a Glycine soja Ca(2+)ATPase in salt-alkaline stress responses.
abstract: It is widely accepted that Ca(2+)ATPase family proteins play important roles in plant environmental stress responses. However, up to now, most researches are limited in the reference plants Arabidopsis and rice. The function of Ca(2+)ATPases from non-reference plants was rarely reported, especially its regulatory role in carbonate alkaline stress responses. Hence, in this study, we identified the P-type II Ca(2+)ATPase family genes in soybean genome, determined their chromosomal location and gene architecture, and analyzed their amino acid sequence and evolutionary relationship. Based on above results, we pointed out the existence of gene duplication for soybean Ca(2+)ATPases. Then, we investigated the expression profiles of the ACA subfamily genes in wild soybean (Glycine soja) under carbonate alkaline stress, and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses, as evidenced by lower levels of membrane permeability and MDA content, but higher levels of SOD activity, proline concentration and chlorophyll content under stress conditions. Taken together, for the first time, we reported a P-type II Ca(2+)ATPase from wild soybean, GsACA1, which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.|Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Sun
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.|Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Jia
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Cui
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Huizi
authors.initials: H
authors.lastname: Duanmu
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Qingyue
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Jialei
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China. sunxiaoli2008@aliyun.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ymzhu@neau.edu.cn.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D002254:Carbonates / Q000633:toxicity; D019143:Evolution, Molecular; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000633:toxicity*; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D002254:Carbonates; D010940:Plant Proteins; D012965:Sodium Chloride; D000252:Calcium-Transporting ATPases
keywords: Alfalfa; Ca2+ATPase; Carbonate alkaline stress; Glycine soja; Salt stress