title: Oxidative stress level in fresh ejaculate is not related to semen parameters or to pregnancy rates in cycles with donor oocytes.
abstract: OBJECTIVE
  The purpose of the present study is to study the relationship between oxidative stress (OS) in semen, semen characteristics, and reproductive outcomes in oocyte donation intracytoplasmic sperm injection (ICSI) cycles.
  
  
  METHODS
  OS was measured in 132 semen samples.
  
  
  RESULTS
  OS levels were as follows: very high (1.5 %), high (43.2 %), low (30.3 %), and very low (25.0 %). Overall seminal parameters were as follows: volume (ml) = 4.2 (SD 2.1), concentration (millions/ml) = 61.6 (SD 59.8), motility (a+b%) = 47.4 (SD 18.0), and normal spermatozoa (%) = 8.2 (SD 5.1). Of the 101 cycles that reached embryo transfer, 55.4 % evolved in biochemical, 46.5 % in clinical, and 43.6 % in ongoing pregnancy. OS level does not relate to seminal parameters, fertilization rate, or pregnancy outcomes.
  
  
  CONCLUSIONS
  OS testing by nitro blue tetrazolium (NBT) in fresh ejaculate might not be useful for all patients. Reproductive results with young oocytes and ICSI do not seem to be affected by OS-level semen.
authors.affiliation: Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.
authors.forename: Aïda
authors.initials: A
authors.lastname: Pujol
authors.affiliation: Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Obradors
authors.affiliation: Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.
authors.forename: Erica
authors.initials: E
authors.lastname: Esteo
authors.affiliation: Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Costilla
authors.affiliation: Fundació Privada EUGIN, 08029, Barcelona, Spain.
authors.forename: Désireé
authors.initials: D
authors.lastname: García
authors.affiliation: Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.
authors.forename: Valerie
authors.initials: V
authors.lastname: Vernaeve
authors.affiliation: Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain. rvassena@eugin.es.
authors.forename: Rita
authors.initials: R
authors.lastname: Vassena
mesh_terms: D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism / Q000473:pathology; D008297:Male; D018587:Oocyte Donation; D009865:Oocytes / Q000378:metabolism*; D018384:Oxidative Stress; D011247:Pregnancy; D011256:Pregnancy Outcome; D012661:Semen / Q000378:metabolism; D020554:Sperm Injections, Intracytoplasmic
keywords: ICSI; Oocyte donation; Oxidative stress; Pregnancy rates; Sperm