title: Analyses of volatiles produced by the African fruit fly species complex (Diptera, Tephritidae).
abstract: Ceratitis fasciventris, Ceratitis anonae and Ceratitis rosa are polyphagous agricultural pests originating from the African continent. The taxonomy of this group (the so-called Ceratitis FAR complex) is unclear. To clarify the taxonomic relationships, male and female-produced volatiles presumably involved in pre-mating communication were studied using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC×GC-TOFMS) followed by multivariate analysis, and gas chromatography combined with electroantennographic detection (GC-EAD). GC×GC-TOFMS analyses revealed sex specific differences in produced volatiles. Male volatiles are complex mixtures that differ both qualitatively and quantitatively but share some common compounds. GC-EAD analyses of male volatiles revealed that the antennal sensitivities of females significantly differ in the studied species. No female volatiles elicited antennal responses in males. The results show clear species-specific differences in volatile production and provide complementary information for the distinct delimitation of the putative species by chemotaxonomic markers.
authors.affiliation: Institute of Organic Chemistry and Biochemistry ASCR, v.v.i., Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic; Institute of Chemical Technology in Prague, Technická 5, CZ-166 28 Prague 6, Czech Republic.
authors.forename: Radka
authors.initials: R
authors.lastname: Břízová
authors.affiliation: Institute of Organic Chemistry and Biochemistry ASCR, v.v.i., Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic; Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro, CEP 57072-970, Maceió, AL, Brazil.
authors.forename: Lucie
authors.initials: L
authors.lastname: Vaníčková
authors.affiliation: Charles University, Albertov 6, CZ-128 43 Prague 2, Czech Republic.
authors.forename: Mária
authors.initials: M
authors.lastname: Faťarová
authors.affiliation: International Centre of Insect Physiology and Ecology, PO Box 30772-00100 GPO, Nairobi, Kenya.
authors.forename: Sunday
authors.initials: S
authors.lastname: Ekesi
authors.affiliation: Institute of Organic Chemistry and Biochemistry ASCR, v.v.i., Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Hoskovec
authors.affiliation: Institute of Organic Chemistry and Biochemistry ASCR, v.v.i., Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic; Czech University of Life Sciences, Kamýcká 129, CZ-165 21 Prague 6, Czech Republic.
authors.forename: Blanka
authors.initials: B
authors.lastname: Kalinová
keywords: Ceratitis FAR complex; GC-EAD; GC×GC-TOFMS; chemotaxonomy; male and female-borne volatiles