title: Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.
abstract: Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation. The three Rab family members showed different localization patterns in oocytes. Microinjection of siRNA, antibody injection, or inhibitor treatment showed that (1) Rab3A regulates peripheral spindle and cortical granule (CG) migration, polarity establishment, and asymmetric division; (2) Rab27A regulates CG exocytosis following MII-stage oocyte activation; and (3) Rab35 plays an important role in spindle organization and morphology maintenance, and thus meiotic nuclear maturation. These results show that Rab proteins play important roles in mouse oocyte meiotic maturation and activation and that different members exert different distinct functions.
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.|Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: H H
authors.initials: HH
authors.lastname: Wang
authors.affiliation: Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.|Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Ouyang
authors.affiliation: Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: W
authors.initials: W
authors.lastname: Shen
authors.affiliation: Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO, 65211, USA.
authors.forename: H
authors.initials: H
authors.lastname: Schatten
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China. sunqy@ioz.ac.cn.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. sunqy@ioz.ac.cn.|Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China. sunqy@ioz.ac.cn.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D008540:Meiosis; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000075745:rab27 GTP-Binding Proteins; D020826:rab3A GTP-Binding Protein / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000075745:rab27 GTP-Binding Proteins; C543587:Rab35 protein, mouse; C512962:Rab27a protein, mouse; D020691:rab GTP-Binding Proteins; D020826:rab3A GTP-Binding Protein
keywords: Cortical granules; Meiotic maturation; Rab27A; Rab35; Rab3A