title: Sheet of osteoblastic cells combined with platelet-rich fibrin improves the formation of bone in critical-size calvarial defects in rabbits.
abstract: Techniques that use sheets of cells have been successfully used in various types of tissue regeneration, and platelet-rich fibrin (PRF) can be used as a source of growth factors to promote angiogenesis. We have investigated the effects of the combination of PRF and sheets of mesenchymal stem cells (MSC) from bone marrow on the restoration of bone in critical-size calvarial defects in rabbits to find out whether the combination promotes bony healing. Sheets of MSC and PRF were prepared from the same donor. We then implanted the combined MSC and PRF in critical-size calvarial defects in rabbits and assessed bony restoration by microcomputed tomography (microCT) and histological analysis. The results showed that PRF significantly increased bony regeneration at 8 weeks after implantation of sheets of MSC and PRF compared with sheets of MSC alone (p=0.0048). Our results indicate that the combination of sheets of MSC and PRF increases bone regeneration in critical-size calvarial defects in rabbits, and provides a new way to improve skeletal healing.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhifa
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Stomatology, the 94(th) hospital of PLA, NanChang, Jiangxi Province 330002, China.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhijin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, Wuhan General Hopital of Guangzhou Military Region, Wuhan 43000, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Weng
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Taiqiang
authors.initials: T
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zong
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China. Electronic address: liuyanpu@fmmu.edu.cn.
authors.forename: Yanpu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral Biology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China. Electronic address: kqomsliu@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D001792:Blood Platelets; D001861:Bone Regeneration; D005337:Fibrin; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D011817:Rabbits; D012886:Skull; D055114:X-Ray Microtomography
chemical_list: D005337:Fibrin
keywords: Bone regeneration; Cell sheet; Mesenchymal stem cells; Platelet-rich fibrin; Rabbit calvarial critical-size defect