title: Evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation.
abstract: The study of sexual behavior and the identification of the signals involved in mate recognition between con-specifics are key components that can shed some light, as part of an integrative taxonomic approach, in delimitating species within species complexes. In the Tephritidae family several species complexes have received particular attention as they include important agricultural pests such as the Ceratitis fasciventris (Bezzi), Ceratitis anonae (Graham) and Ceratitis rosa Karsch (FAR) complex, the Bactrocera dorsalis (Hendel) complex and the Anastrepha fraterculus (Wiedemann) complex. Here the value and usefulness of a methodology that uses walk-in field cages with host trees to assess, under semi-natural conditions, mating compatibility within these complexes is reviewed, and the same methodology to study the role of chemical communication in pre-mating isolation among Anastrepha fraterculus populations is used. Results showed that under the same experimental conditions it was possible to distinguish an entire range of different outcomes: from full mating compatibility among some populations to complete assortative mating among others. The effectiveness of the methodology in contributing to defining species limits was shown in two species complexes: Anastrepha fraterculus and Bactrocera dorsalis, and in the case of the latter the synonymization of several established species was published. We conclude that walk-in field cages constitute a powerful tool to measure mating compatibility, which is also useful to determine the role of chemical signals in species recognition. Overall, this experimental approach provides a good source of information about reproductive boundaries to delimit species. However, it needs to be applied as part of an integrative taxonomic approach that simultaneously assesses cytogenetic, molecular, physiological and morphological traits in order to reach more robust species delimitations.
authors.affiliation: Cátedra Terapéutica Vegetal, Facultad de Agronomía y Zootecnia (FAZ), Universidad Nacional de Tucumán (UNT), San Miguel de Tucumán; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: M Laura
authors.initials: ML
authors.lastname: Juárez
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Argentina.
authors.forename: Francisco
authors.initials: F
authors.lastname: Devescovi
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic.
authors.forename: Radka
authors.initials: R
authors.lastname: Břízová
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Argentina.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Bachmann
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Argentina.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Segura
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic.
authors.forename: Blanka
authors.initials: B
authors.lastname: Kalinová
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Argentina.
authors.forename: Patricia
authors.initials: P
authors.lastname: Fernández
authors.affiliation: Cátedra Terapéutica Vegetal, Facultad de Agronomía y Zootecnia (FAZ), Universidad Nacional de Tucumán (UNT), San Miguel de Tucumán; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: M Josefina
authors.initials: MJ
authors.lastname: Ruiz
authors.affiliation: Fujian Agriculture and Forestry University, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Chemistry Research Unit, USDA-ARS, Gainesville, USA.
authors.forename: Peter E A
authors.initials: PE
authors.lastname: Teal
authors.affiliation: Insect Pest Control Laboratory (IPCL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
authors.forename: Carlos
authors.initials: C
authors.lastname: Cáceres
authors.affiliation: Insect Pest Control Laboratory (IPCL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
authors.forename: Marc J B
authors.initials: MJ
authors.lastname: Vreysen
authors.affiliation: Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
authors.forename: Jorge
authors.initials: J
authors.lastname: Hendrichs
authors.affiliation: Cátedra Terapéutica Vegetal, Facultad de Agronomía y Zootecnia (FAZ), Universidad Nacional de Tucumán (UNT), San Miguel de Tucumán; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Vera
keywords: Anastrepha fraterculus; Bactrocera dorsalis; Ceratitis anonae; Tephritidae; field cages; species delimitation; ﻿ Ceratitis fasciventris; ﻿ Ceratitis rosa