title: Analysis of Hippo and TGFβ signaling in polarizing epithelial cells and mouse embryos.
abstract: The Hippo signaling pathway is involved in numerous biological events ranging from early development to organogenesis and when disrupted, impacts various human diseases including cancer. The Hippo pathway also interacts with and controls the activity of other signaling pathways such as the TGFβ/Smad pathway, in which Hippo pathway activity influences the subcellular localization of Smad transcription factors. Here, we describe techniques for examining crosstalk between Hippo and TGFβ signaling in polarizing mammary epithelial cells. In addition, we provide detailed methods for analyzing the subcellular localization of the Hippo pathway effectors, Taz and Yap using both in vitro cultured epithelial cells and in vivo in pregastrulation mouse embryos.
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ont., Canada.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Narimatsu
authors.affiliation: Department of Biochemistry and Donnelly Centre, University of Toronto, Canada.
authors.forename: Batool
authors.initials: B
authors.lastname: Labibi
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ont., Canada.
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Wrana
authors.affiliation: Department of Biochemistry and Donnelly Centre, University of Toronto, Canada. Electronic address: liliana.attisano@utoronto.ca.
authors.forename: Liliana
authors.initials: L
authors.lastname: Attisano
mesh_terms: D000217:Acyltransferases; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000818:Animals; D018797:Cell Cycle Proteins; D016764:Cell Polarity / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology*; D000090683:Hippo Signaling Pathway; D051379:Mice; D009024:Morphogenesis / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D015398:Signal Transduction; D051785:Smad Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics*; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D010750:Phosphoproteins; D051785:Smad Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D000091102:YAP-Signaling Proteins; C486223:Yap1 protein, mouse; D000217:Acyltransferases; C486363:tafazzin protein, mouse; D017346:Protein Serine-Threonine Kinases
keywords: Apical-basal polarity; EpH4 cells; Hippo; Immunofluorescence microscopy; Mouse embryos; TGFβ