title: Revisiting a protocol for extraction of mycobacterial lipids.
abstract: Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India; Department of Biochemistry, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Sinha
authors.affiliation: Department of Biochemistry, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Tandon
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Tyagi
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Khatri
authors.affiliation: Department of Chemistry, University of Delhi, Delhi 110007, India.
authors.forename: L
authors.initials: L
authors.lastname: Chandra Shekhar Reddy
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Neeraj K
authors.initials: NK
authors.lastname: Saini
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Pathak
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
authors.forename: Mandira
authors.initials: M
authors.lastname: Varma-Basil
authors.affiliation: Department of Chemistry, University of Delhi, Delhi 110007, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Prasad
authors.affiliation: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: bosemridula@yahoo.co.in.
authors.forename: Mridula
authors.initials: M
authors.lastname: Bose
keywords: M. tuberculosis; Thin layer chromatography; Total lipids