title: Cellular prion protein regulates invasion and migration of breast cancer cells through MMP-9 activity.
abstract: Function of cellular prion protein (PrP(c)) in cancer progression has not been elucidated yet. Ectopic expression of PrP(c) increases the invasion and migration of breast cancer cell line, MCF-7 cells. Overexpressed PrP(c) increases matrix metalloprotease-9 (MMP-9) expression by enhancing association of NF-κB in promoter of MMP-9 gene and ERK signaling in MCF-7 cells. Whereas, silencing of PrP(c) by siRNA suppresses ERK activation and MMP-9 expression resulting the down-regulation of MD-MB231 cell migration and invasion. Overall, these results suggest that PrP(c) contribute the breast cancer invasion and migration via MMP-9.
authors.affiliation: SIS Immunology Research Institute, Sookmyung Women's University, Seoul, South Korea.
authors.forename: Minchan
authors.initials: M
authors.lastname: Gil
authors.affiliation: Department of Convergence Medicine, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
authors.forename: Yun Kye
authors.initials: YK
authors.lastname: Kim
authors.affiliation: SIS Immunology Research Institute, Sookmyung Women's University, Seoul, South Korea.
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Kim
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, South Korea.
authors.forename: Wook
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Pathology, Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: csikpark@amc.seoul.kr.
authors.forename: Chan-Sik
authors.initials: CS
authors.lastname: Park
authors.affiliation: Department of Convergence Medicine, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 138-736, South Korea. Electronic address: kjlee@amc.seoul.kr.
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Lee
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002465:Cell Movement; D004789:Enzyme Activation; D006801:Humans; D020935:MAP Kinase Signaling System; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D018621:PrPC Proteins / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D018621:PrPC Proteins; D020780:Matrix Metalloproteinase 9
keywords: Breast cancer; Invasion; Matrix metalloprotease-9; Migration; Prion