title: Oxidative stress in follicular fluid of young women with low response compared with fertile oocyte donors.
abstract: The aim of this study was to determine the concentrations of oxidative stress markers, antioxidant enzymes and cytokines in the follicular fluid of young women with low response in ovarian stimulation cycles compared with high responders and fertile oocyte donors of the same age, to assess the impact of oxidative stress on ovarian reserve. The activity of follicular fluid antioxidant enzymes glutathione transferase, glutathione reductase and glutathione peroxidase was significantly lower in young women with reduced ovarian reserve compared with that in high responders and oocyte donors. Follicular fluid concentrations of oxidative stress marker malondialdehyde combined with 4-hydroxyalkenals and nitric oxide were higher in low responders than in high responders and oocyte donors. Significant differences between low responders and donors in concentrations of IL-2, IL-6, IL-8 and vascular endothelial growth factor were observed, with higher concentrations in low responders. However, IL-10 concentration was lower in low responders than in high responders and donors. No significant differences were found in follicular fluid concentrations of tumour necrosis factor alpha between the three groups. These results demonstrate that different concentrations of oxidative stress markers, oxidant enzymes and cytokines in low responders compared with high responders and oocyte donors may negatively impact ovarian response.
authors.affiliation: Clínica Tambre, Madrid, Spain. Electronic address: rocio@clinicatambre.com.
authors.forename: Rocío
authors.initials: R
authors.lastname: Nuñez-Calonge
authors.affiliation: Clínica Tambre, Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Cortés
authors.affiliation: Department Physiology, Medical School, University Complutense of Madrid, Madrid, Spain.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Gutierrez Gonzalez
authors.affiliation: Department Physiology, Medical School, University Complutense of Madrid, Madrid, Spain; Instituto de Investigación Biomédica de Vigo (IBIV), Xerencia de Xestión Integrada de Vigo, SERGAS, Vigo, Spain.
authors.forename: Roman
authors.initials: R
authors.lastname: Kireev
authors.affiliation: Department of Biochemistry and Mollecular Biology Medical School, University Complutense of Madrid, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Vara
authors.affiliation: Clínica Tambre, Madrid, Spain.
authors.forename: Leonor
authors.initials: L
authors.lastname: Ortega
authors.affiliation: Clínica Tambre, Madrid, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Caballero
authors.affiliation: Department Physiology, Medical School, University Complutense of Madrid, Madrid, Spain.
authors.forename: Lisa
authors.initials: L
authors.lastname: Rancan
authors.affiliation: Department Physiology, Medical School, University Complutense of Madrid, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Tresguerres
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D018587:Oocyte Donation; D065851:Ovarian Reserve; D010062:Ovulation Induction; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D007378:Interleukins; D009569:Nitric Oxide; D008315:Malondialdehyde
keywords: follicular fluid; low ovarian reserve; oxidative stress