title: Nutritional and Biochemical Profiling of Leucopaxillus candidus (Bres.) Singer Wild Mushroom.
abstract: The wild mushroom Leucopaxillus candidus (Bres.) Singer was studied for the first time to obtain information about its chemical composition, nutritional value and bioactivity. Free sugars, fatty acids, tocopherols, organic and phenolic acids were analysed by chromatographic techniques coupled to different detectors. L. candidus methanolic extract was tested regarding antioxidant potential (reducing power, radical scavenging activity and lipid peroxidation inhibition). L. candidus was shown to be an interesting species in terms of nutritional value, with high content in proteins and carbohydrates, but low fat levels, with the prevalence of polyunsaturated fatty acids. Mannitol was the most abundant free sugar and β-tocopherol was the main tocopherol isoform. Other compounds detected were oxalic and fumaric acids, p-hydroxybenzoic and cinnamic acids. The methanolic extract revealed antioxidant activity and did not show hepatoxicity in porcine liver primary cells. The present study provides new information about L. candidus.
authors.affiliation: Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, Bragança 5301-855, Portugal. vieira.aa.vanessa@gmail.com.|School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Ap. 1172, Bragança 5301-855, Portugal. vieira.aa.vanessa@gmail.com.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Vieira
authors.affiliation: Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, Bragança 5301-855, Portugal. lillian@ipb.pt.
authors.forename: Lillian
authors.initials: L
authors.lastname: Barros
authors.affiliation: School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Ap. 1172, Bragança 5301-855, Portugal. amartins@ipb.pt.
authors.forename: Anabela
authors.initials: A
authors.lastname: Martins
authors.affiliation: Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, Bragança 5301-855, Portugal. iferreira@ipb.pt.|School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Ap. 1172, Bragança 5301-855, Portugal. iferreira@ipb.pt.
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000302:isolation & purification / Q000378:metabolism; D045424:Complex Mixtures / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000378:metabolism; D005650:Fumarates / Q000302:isolation & purification / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D008353:Mannitol / Q000302:isolation & purification* / Q000378:metabolism; D055442:Metabolome; D009753:Nutritive Value; D010070:Oxalates / Q000302:isolation & purification / Q000378:metabolism; D010226:Parabens / Q000302:isolation & purification / Q000378:metabolism; D010851:Picrates / Q000037:antagonists & inhibitors; D061251:Primary Cell Culture; D013552:Swine; D024503:beta-Tocopherol / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002934:Cinnamates; D045424:Complex Mixtures; D005231:Fatty Acids, Unsaturated; D005650:Fumarates; D010070:Oxalates; D010226:Parabens; D010851:Picrates; C029010:cinnamic acid; D008353:Mannitol; C032005:fumaric acid; D024503:beta-Tocopherol; C004931:1,1-diphenyl-2-picrylhydrazyl; C038193:4-hydroxybenzoic acid
keywords: Leucopaxillus candidus; antioxidant activity; chemical composition; wild mushroom