title: Immunization against an IL-6 peptide induces anti-IL-6 antibodies and modulates the Delayed-Type Hypersensitivity reaction in cynomolgus monkeys.
abstract: Interleukin-6 (IL-6) overproduction has been involved in the pathogenesis of several chronic inflammatory diseases and the administration of an anti-IL-6 receptor monoclonal antibody has been proven clinically efficient to treat them. However, the drawbacks of monoclonal antibodies have led our group to develop an innovative anti-IL-6 strategy using a peptide-based active immunization. This approach has previously shown its efficacy in a mouse model of systemic sclerosis. Here the safety, immunogenicity, and efficacy of this strategy was assessed in non human primates. No unscheduled death and clinical signs of toxicity was observed during the study. Furthermore, the cynomolgus monkeys immunized against the IL-6 peptide produced high levels of anti-IL-6 antibodies as well as neutralizing antibodies compared to control groups. They also showed an important decrease of the cumulative inflammatory score following a delayed-type hypersensitivity reaction induced by the Tetanus vaccine compared to control groups (minus 57,9%, P = 0.014). These findings are highly significant because the immunizing IL-6 peptide used in this study is identical in humans and in monkeys and this novel anti-IL-6 strategy could thus represent a promising alternative to monoclonal antibodies.
authors.affiliation: Laboratoire Génomique, Bioinformatique et Applications, EA 4627, Chaire de Bioinformatique, Conservatoire National des Arts et Métiers, 292 rue Saint Martin, 75003 Paris, France.
authors.forename: Lucille
authors.initials: L
authors.lastname: Desallais
authors.affiliation: Toxicologist, Nice, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Bouchez
authors.affiliation: Laboratoire Génomique, Bioinformatique et Applications, EA 4627, Chaire de Bioinformatique, Conservatoire National des Arts et Métiers, 292 rue Saint Martin, 75003 Paris, France.
authors.forename: Hadley
authors.initials: H
authors.lastname: Mouhsine
authors.affiliation: Peptinov, 29 rue du Faubourg Saint-Jacques, Pépinière Paris Santé Cochin, Hôpital Cochin, 75014 Paris, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Moreau
authors.affiliation: Peptinov, 29 rue du Faubourg Saint-Jacques, Pépinière Paris Santé Cochin, Hôpital Cochin, 75014 Paris, France.
authors.forename: Rojo
authors.initials: R
authors.lastname: Ratsimandresy
authors.affiliation: Laboratoire Génomique, Bioinformatique et Applications, EA 4627, Chaire de Bioinformatique, Conservatoire National des Arts et Métiers, 292 rue Saint Martin, 75003 Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Montes
authors.affiliation: Peptinov, 29 rue du Faubourg Saint-Jacques, Pépinière Paris Santé Cochin, Hôpital Cochin, 75014 Paris, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Do
authors.affiliation: INSERM U970 PARCC (Paris Cardiovascular Research Center); Université Paris Descartes; Sorbonne Paris Cité; Paris, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Quintin-Colonna
authors.affiliation: Laboratoire Génomique, Bioinformatique et Applications, EA 4627, Chaire de Bioinformatique, Conservatoire National des Arts et Métiers, 292 rue Saint Martin, 75003 Paris, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Zagury
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007114:Immunization / Q000379:methods; D015850:Interleukin-6 / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D019947:Receptors, Interleukin-6 / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D015850:Interleukin-6; D010455:Peptides; D019947:Receptors, Interleukin-6