title: Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay.
abstract: Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called "Simple Western" was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Poultry Microbiological Safety and Processing Research Unit, 950 College Station Road, Athens, GA 30605-2720, USA. Electronic address: hungyueh.yeh@ars.usda.gov.
authors.forename: Hung-Yueh
authors.initials: HY
authors.lastname: Yeh
authors.affiliation: Food Science and Technology Program, Faculty of Agricultural Sciences, University of Puerto Rico Mayagüez Campus, P.O. Box 9000, Mayagüez, PR 00681-9000, USA.
authors.forename: Katherine Vargas
authors.initials: KV
authors.lastname: Serrano
authors.affiliation: Food Science and Technology Program, Faculty of Agricultural Sciences, University of Puerto Rico Mayagüez Campus, P.O. Box 9000, Mayagüez, PR 00681-9000, USA.
authors.forename: Aimee Silvestry
authors.initials: AS
authors.lastname: Acosta
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Poultry Microbiological Safety and Processing Research Unit, 950 College Station Road, Athens, GA 30605-2720, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Buhr
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001331:Automation; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D001483:Base Sequence; D002645:Chickens / Q000097:blood / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D019779:Salmonella enterica / Q000235:genetics / Q000276:immunology*; D015047:Zoonoses / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C088595:FlgK protein, Bacteria; D011994:Recombinant Proteins
keywords: Flagellar proteins; FlgK; Salmonella enterica; Simple Western; Zoonoses