title: Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells.
abstract: Oral squamous cell carcinoma (OSCC), the most frequent of all oral cancers, is a type of highly malignant tumors with a high capacity to invade locally and form distant metastases. An increasing number of studies have shown that microRNAs (miRNAs) play an important role in regulating cancer metastasis and invasion. In the present study, we detected the expression of miR-221 in two highly metastatic OSCC cell lines and two OSCC cell lines that are less metastatic using quantitative real-time PCR analysis (qRT-PCR). The qRT-PCR results indicate that miR-221 is upregulated in highly metastatic OSCC cell lines. Then, miR-221 expression was knocked down by transfection with miR-221 inhibitor, and UM1 cell migration and invasion were assessed using transwell migration and invasion assays. The results indicate that inhibition of miR-221 suppressed migration and invasion of UM1 cells. Furthermore, methyl-CpG binding domain protein 2 (MBD2) was identified as a direct target gene of miR-221. Additionally, MBD2 silencing could partly reverse the effect of miR-221 on cell migration and invasion. In conclusion, downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1.
authors.affiliation: The Medical Centre of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Shuqi
authors.initials: S
authors.lastname: He
authors.affiliation: The Medical Centre of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Renfa
authors.initials: R
authors.lastname: Lai
authors.affiliation: Department of Oral & Maxillofacial Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Oral & Maxillofacial Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Wangxiang
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Stomatology, Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Oral & Maxillofacial Surgery, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Oral & Maxillofacial Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Oral & Maxillofacial Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics; D015398:Signal Transduction
chemical_list: D004268:DNA-Binding Proteins; D035683:MicroRNAs; C489325:MBD2 protein, human; C525709:MIR221, human