title: Supported liquid membrane-protected molecularly imprinted beads for the solid phase micro-extraction of triazines from environmental waters.
abstract: In this work, a novel methodology based on the combination of MIP technology with micro solid-phase extraction in a hollow fibre device for the trace enrichment and cleanup of triazines in environmental waters is described. All parameters affecting both migration of triazines from samples to the lumen of the fibre and rebinding into MIP sites have been carefully optimized. Final conditions included, addition of 20% NaCl to the sample to produce salting-out effect, extraction of analytes during 45min with orbital stirring at 750rpm, and then washing the fibre with toluene during 5min to allow selective recognition of triazines before elution in HPLC inserts containing 450μL of a methanol/acetic acid mixture (95/5, v/v). Under optimum conditions, quantitative recoveries for simazine, cyanazine, atrazine, propazine and terbutylazine, were achieved both in surface, ground and tap water samples, with relative standard deviations lower than 10.6%, and limits of detection in the low ngL(-1) concentration level.
authors.affiliation: Departamento de Medio Ambiente, INIA, Carretera de A Coruña km 7.5, 28240 Madrid, Spain. Electronic address: turiel@inia.es.
authors.forename: E
authors.initials: E
authors.lastname: Turiel
authors.affiliation: Departamento de Medio Ambiente, INIA, Carretera de A Coruña km 7.5, 28240 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Díaz-Álvarez
authors.affiliation: Departamento de Medio Ambiente, INIA, Carretera de A Coruña km 7.5, 28240 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martín-Esteban
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005618:Fresh Water / Q000737:chemistry*; D006540:Herbicides / Q000302:isolation & purification*; D057230:Limit of Detection; D008567:Membranes, Artificial; D054802:Molecular Imprinting; D011109:Polymethacrylic Acids / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods; D014227:Triazines / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D006540:Herbicides; D008567:Membranes, Artificial; D011109:Polymethacrylic Acids; D014227:Triazines; D014874:Water Pollutants, Chemical; C030613:polymethacrylic acid
keywords: Aqueous samples; Imprinted polymers; Micro-solid phase extraction; Miniaturization; Supported liquid membrane; Triazines