title: PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root.
abstract: In plants, the generation of new cell types and tissues depends on coordinated and oriented formative cell divisions. The plasma membrane-localized receptor kinase ARABIDOPSIS CRINKLY 4 (ACR4) is part of a mechanism controlling formative cell divisions in the Arabidopsis root. Despite its important role in plant development, very little is known about the molecular mechanism with which ACR4 is affiliated and its network of interactions. Here, we used various complementary proteomic approaches to identify ACR4-interacting protein candidates that are likely regulators of formative cell divisions and that could pave the way to unraveling the molecular basis behind ACR4-mediated signaling. We identified PROTEIN PHOSPHATASE 2A-3 (PP2A-3), a catalytic subunit of PP2A holoenzymes, as a previously unidentified regulator of formative cell divisions and as one of the first described substrates of ACR4. Our in vitro data argue for the existence of a tight posttranslational regulation in the associated biochemical network through reciprocal regulation between ACR4 and PP2A-3 at the phosphorylation level.
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Kun
authors.initials: K
authors.lastname: Yue
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011;
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sandal
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
authors.forename: Elisabeth L
authors.initials: EL
authors.lastname: Williams
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
authors.forename: Evan
authors.initials: E
authors.lastname: Murphy
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium; Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium;
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Stes
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Natalia
authors.initials: N
authors.lastname: Nikonorova
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
authors.forename: Priya
authors.initials: P
authors.lastname: Ramakrishna
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
authors.forename: Nathan
authors.initials: N
authors.lastname: Czyzewicz
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Laura
authors.initials: L
authors.lastname: Montero-Morales
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Robert
authors.initials: R
authors.lastname: Kumpf
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
authors.forename: Zhefeng
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Brigitte
authors.initials: B
authors.lastname: van de Cotte
authors.affiliation: Multidisciplinary Centre for Integrative Biology, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
authors.forename: Mudassar
authors.initials: M
authors.lastname: Iqbal
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Michiel
authors.initials: M
authors.lastname: Van Bel
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Eveline
authors.initials: E
authors.lastname: Van De Slijke
authors.affiliation: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63130;
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Meyer
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Astrid
authors.initials: A
authors.lastname: Gadeyne
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom;
authors.forename: Cyril
authors.initials: C
authors.lastname: Zipfel
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Geert
authors.initials: G
authors.lastname: De Jaeger
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; ive.desmet@psb.vib-ugent.be marc.vanmontagu@ugent.be.
authors.forename: Marc
authors.initials: M
authors.lastname: Van Montagu
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Daniël
authors.initials: D
authors.lastname: Van Damme
authors.affiliation: Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium; Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium;
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011;
authors.forename: A Gururaj
authors.initials: AG
authors.lastname: Rao
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom; Centre for Plant Integrative Biology, University of Nottingham, Loughborough LE12 5RD, United Kingdom ive.desmet@psb.vib-ugent.be marc.vanmontagu@ugent.be.
authors.forename: Ive
authors.initials: I
authors.lastname: De Smet
mesh_terms: D017360:Arabidopsis / Q000166:cytology*; D029681:Arabidopsis Proteins / Q000502:physiology*; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000502:physiology*; D010766:Phosphorylation; D018517:Plant Roots / Q000166:cytology*; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D011956:Receptors, Cell Surface; C462019:ACR4 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases
keywords: columella; kinase; phosphatase; phosphorylation; stem cells