title: Functional characterization of TRPM7 in nasopharyngeal carcinoma and its knockdown effects on tumorigenesis.
abstract: The aim of this study was to evaluate the association of functional expression of TRPM7 with nasopharyngeal carcinoma (NPC) growth. We examined the correlation of TRPM7 expression with cell growth and proliferation, cell cycle, and apoptosis in vitro in NPC cell lines and NPC tumorigenesis in mice by conducting experiments in mice and by further analyzing the tumor volume and growth. We further explored to see whether there is any positive correlation with the TRPM7 knockdown in NPC cells with their sensitivity to radiation. We found that the functional expression of TRPM7 in nasopharyngeal carcinoma is a critical requirement for physiological processes such as cell cycle, resistance to apoptosis, and cell proliferation. TRPM7 knockdown also enhanced sensitivity to radiotherapy of nasopharyngeal carcinoma. Moreover, we identified TRPM7 as a novel potential regulator of cell proliferation in NPC, through signal transducer and activator of transcription 3 (STAT3)-mediated signaling pathway and other anti-apoptotic factors. TRPM7 and STAT3 activation might be critical for the growth of NPC cells and could be an effective target for treatment of nasopharyngeal carcinoma.
authors.affiliation: Department of Orthopedics, Zhuhai People's Hospital, Zhuhai, 519000, China.|Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Liao
authors.affiliation: Forevergen Biosciences Center, R&D Unit 602, Guangzhou, 510000, China.
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Luo
authors.affiliation: Forevergen Biosciences Center, R&D Unit 602, Guangzhou, 510000, China.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Wu
authors.affiliation: Forevergen Biosciences Center, R&D Unit 602, Guangzhou, 510000, China.
authors.forename: An-Shang
authors.initials: AS
authors.lastname: Lu
authors.affiliation: Forevergen Biosciences Center, R&D Unit 602, Guangzhou, 510000, China.
authors.forename: Pu-Xia
authors.initials: PX
authors.lastname: Su
authors.affiliation: Forevergen Biosciences Center, R&D Unit 602, Guangzhou, 510000, China. lbq@forevergen.cn.
authors.forename: Bing-Quan
authors.initials: BQ
authors.lastname: Lai
authors.affiliation: Department of Medical Oncology, State Key Laboratory of Oncology in South China, and Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. wangxiaoxiao806@sina.com.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D002277:Carcinoma; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D050053:TRPM Cation Channels / Q000235:genetics*; D047368:Tumor Burden / Q000235:genetics
chemical_list: D050796:STAT3 Transcription Factor; D050053:TRPM Cation Channels; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human
keywords: Apoptosis; CNE-1 cells; Nasopharyngeal carcinoma; Prognosis; Radiotherapy; STAT3; TRPM7