title: USPIO-enhanced 3D-cine self-gated cardiac MRI based on a stack-of-stars golden angle short echo time sequence: Application on mice with acute myocardial infarction.
abstract: To develop and assess a 3D-cine self-gated method for cardiac imaging of murine models.
  
  
  
  A 3D stack-of-stars (SOS) short echo time (STE) sequence with a navigator echo was performed at 7T on healthy mice (n = 4) and mice with acute myocardial infarction (MI) (n = 4) injected with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. In all, 402 spokes were acquired per stack with the incremental or the golden angle method using an angle increment of (360/402)° or 222.48°, respectively. A cylindrical k-space was filled and repeated with a maximum number of repetitions (NR) of 10. 3D cine cardiac images at 156 μm resolution were reconstructed retrospectively and compared for the two methods in terms of contrast-to-noise ratio (CNR). The golden angle images were also reconstructed with NR = 10, 6, and 3, to assess cardiac functional parameters (ejection fraction, EF) on both animal models.
  
  
  
  The combination of 3D SOS-STE and USPIO injection allowed us to optimize the identification of cardiac peaks on navigator signal and generate high CNR between blood and myocardium (15.3 ± 1.0). The golden angle method resulted in a more homogeneous distribution of the spokes inside a stack (P < 0.05), enabling reducing the acquisition time to 15 minutes. EF was significantly different between healthy and MI mice (P < 0.05).
  
  
  
  The method proposed here showed that 3D-cine images could be obtained without electrocardiogram or respiratory gating in mice. It allows precise measurement of cardiac functional parameters even on MI mice. J. Magn. Reson. Imaging 2016;44:355-365.
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: Aurélien J
authors.initials: AJ
authors.lastname: Trotier
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Castets
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: William
authors.initials: W
authors.lastname: Lefrançois
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: Emeline J
authors.initials: EJ
authors.lastname: Ribot
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Franconi
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Thiaudière
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 Université de Bordeaux, Bordeaux, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Miraux
mesh_terms: D000818:Animals; D055424:Cardiac-Gated Imaging Techniques / Q000379:methods*; D003287:Contrast Media; D003911:Dextrans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D019028:Magnetic Resonance Imaging, Cine / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10
keywords: 3D-cine cardiac imaging; STE; golden angle; mouse; myocardial infarction; self-gated