title: Detection of Different DNA Animal Species in Commercial Candy Products.
abstract: Candy products are consumed all across the world, but there is not much information about their composition. In this study we have used a DNA-based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species-informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly-types, and gummies (20) contained a significantly higher number of species than hard candies (9). We demonstrated the presence of DNA animal species in candy product which allow consumers to make choices and prevent allergic reaction.
authors.affiliation: Laboratory of Genetics of Natural Resources, Area of Genetics, Dept. of Functional Biology, Univ. of Oviedo, Asturias, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Muñoz-Colmenero
authors.affiliation: Sequencing Unit of the Univ. of Oviedo, Asturias, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Martínez
authors.affiliation: Laboratory of Genetics of Natural Resources, Area of Genetics, Dept. of Functional Biology, Univ. of Oviedo, Asturias, Spain.
authors.forename: Agustín
authors.initials: A
authors.lastname: Roca
authors.affiliation: Laboratory of Genetics of Natural Resources, Area of Genetics, Dept. of Functional Biology, Univ. of Oviedo, Asturias, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Garcia-Vazquez
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes; D002182:Candy / Q000032:analysis*; D002417:Cattle; D004247:DNA / Q000032:analysis*; D005399:Fishes; D005512:Food Hypersensitivity / Q000517:prevention & control; D006801:Humans; D016133:Polymerase Chain Reaction; D013552:Swine
chemical_list: D000485:Allergens; D004247:DNA
keywords: DNA markers; PCR cloning; animal species; candy; gelatin