title: Yangjing Capsule Ameliorates Spermatogenesis in Male Mice Exposed to Cyclophosphamide.
abstract: Yangjing capsule (YC), a traditional Chinese compound herbal preparation, has been proven as an effective drug to improve spermatogenesis in clinical practice. However, its pharmacological mechanisms were not fully clarified. This study was designed to investigate the protective effects of YC on spermatogenesis in the mouse model of spermatogenesis dysfunction induced by cyclophosphamide (CP). The administration of YC significantly increased the epididymal index, sperm count, and sperm motility of model mice. Histopathological changes demonstrated that CP caused obvious structural damage to testis, which were reversed by the administration of YC. Results from TUNEL assay showed that treatment with YC dramatically decreased the apoptosis of spermatogenic cell induced by CP. Moreover, YC treatment could inhibit the mRNA and protein expression of Bax to Bcl-2 and also raised expression of AR at both mRNA and protein levels. These data suggest that YC might ameliorate spermatogenesis in male mice exposed to CP through inhibiting the apoptosis of spermatogenic cell and enhancing the actions of testosterone in spermatogenesis.
authors.affiliation: Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Hongle
authors.identifier: 0000-0003-2303-6834
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Baofang
authors.initials: B
authors.lastname: Jin
authors.affiliation: Reproductive Medical Center, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Xindong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yugui
authors.identifier: 0000-0002-2866-6096
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Dalin
authors.initials: D
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.affiliation: Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cai