title: Direct interaction of Ste11 and Mkk1/2 through Nst1 integrates high-osmolarity glycerol and pheromone pathways to the cell wall integrity MAPK pathway.
abstract: Coordination and cross talks of MAPK pathways are critical for signaling efficiency, but their mechanisms are not well understood. Slt2, the MAP kinase of cell wall integrity pathway (CWI), is activated by heat stress even in the absence of upstream components of this pathway, suggesting a supplementary input for Slt2 activation. Here, we identify a new interaction of Ste11 and Mkk1, mediated by Nst1 that connects the high-osmolarity glycerol and pheromone pathways directly to CWI pathway in response to heat and pheromone. We suggest that Ser(407) and Thr(411) are novel residues of Mkk1 activated by these MAPK pathways.
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
authors.forename: Gang
authors.initials: G
authors.lastname: Leng
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Song
mesh_terms: D019943:Amino Acid Substitution; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism; D017353:Gene Deletion; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects; D048369:MAP Kinase Kinase 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020929:Mitogen-Activated Protein Kinase Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010675:Pheromones / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D054730:Protein Interaction Domains and Motifs; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000819:agonists / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D006389:Hemagglutinins, Viral; C000609585:Nst1 protein, S cerevisiae; D010446:Peptide Fragments; D010675:Pheromones; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D020928:Mitogen-Activated Protein Kinases; C072724:SLT2 protein, S cerevisiae; D020930:MAP Kinase Kinase Kinases; C067018:Ste11 protein, S cerevisiae; D048369:MAP Kinase Kinase 1; C561307:MKK2 protein, S cerevisiae; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: MAP kinase cascades; Mkk1/2; Nst1; Slt2; Ste11; budding yeast Saccharomyces cerevisiae; heat stress; pheromone stress; the cell wall integrity pathway