title: Effects and Molecular Mechanism of GST-Irisin on Lipolysis and Autocrine Function in 3T3-L1 Adipocytes.
abstract: Irisin, which was recently identified as a myokine and an adipokine, transforms white adipose tissue to brown adipose tissue and has increasingly caught the attention of the medical and scientific community. However, the signaling pathway of irisin and the molecular mechanisms responsible for the lipolysis effect remain unclear. In this study, we established an efficient system for the expression and purification of GST-irisin in Escherichia coli. The biological activity of GST-irisin was verified using the cell counting kit-8 assay and by detecting the mRNA expression of uncoupling protein 1. Our data showed that GST-irisin regulates mRNA levels of lipolysis-related genes such as adipose triglyceride lipase and hormone-sensitive lipase and proteins such as the fatty acid-binding protein 4, leading to increased secretion of glycerol and decreased lipid accumulation in 3T3-L1 adipocytes. In addition, exogenous GST-irisin can increase its autocrine function in vitro by regulating the expression of fibronectin type III domain-containing protein 5. GST-irisin could regulate glucose uptake in 3T3-L1 adipocytes. Hence, we believe that recombinant GST-irisin could promote lipolysis and its secretion in vitro and can potentially prevent obesity and related metabolic diseases.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.|School of Life Sciences, Jilin University, Changchun, 130012, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.|School of Life Sciences, Jilin University, Changchun, 130012, China.
authors.forename: Fangmin
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jilin University, Changchun, 130012, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.|School of Life Sciences, Jilin University, Changchun, 130012, China.|National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, 130012, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Endocrinology, the Second Hospital of Jilin University, Changchun, 130041, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.|School of Life Sciences, Jilin University, Changchun, 130012, China.|National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, 130012, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D041721:3T3-L1 Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D017667:Adipocytes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D005947:Glucose / Q000032:analysis; D006801:Humans; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins
chemical_list: C571428:FNDC5 protein, mouse; D005353:Fibronectins; D011994:Recombinant Proteins; D005947:Glucose