title: Chip-based visual detection of microRNA using DNA-functionalized gold nanoparticles.
abstract: In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle (AuNP) probes and a gene chip to detect microRNAs (miRNAs). Specific oligonucleotides were attached to the glass surface as capture probes for the target miRNAs, which were then detected via hybridization to the AuNP probes. The signal was amplified via the reduction of HAuCl4 by H2O2. The use of a single AuNP probe detected 10 pmol L(-1) of target miRNA. The recovery rate for miR-126 from fetal bovine serum was 81.5%-109.1%. The biosensor detection of miR-126 in total RNA extracted from lung cancer tissues was consistent with the quantitative PCR (qPCR) results. The use of two AuNP probes further improved the detection sensitivity such that even 1 fmol L(-1) of target miR-125a-5p was detectable. This assay takes less than 1 h to complete and the results can be observed by the naked eye. The platform simultaneously detected lung cancer related miR-126 and miR-125a-5p. Therefore, this low cost, rapid, and convenient technology could be used for ultrasensitive and robust visual miRNA detection.
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, 471003, China.|State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, 471003, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Clinical Research Center of Biological Sensing Technology, Shanghai Xuhui Central Hospital, Shanghai, 200040, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Zule
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Department of Oral and Maxillofacial, School of Stomatology, Dalian Medical University, Dalian, 116023, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China. hjmao@mail.sim.ac.cn.
authors.forename: Hongju
authors.initials: H
authors.lastname: Mao
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, 471003, China. sanqiangli2001@163.com.
authors.forename: Sanqiang
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China. jlzhao@mail.sim.ac.cn.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs; D006046:Gold; D004247:DNA
keywords: biosensor; chip; gold nanoparticles; microRNA; visual detection