title: Studies on water soluble polysaccharides from Pithecellobium dulce (Roxb.) Benth. seeds.
abstract: In this existing experimental work, water soluble PDP polysaccharides were secluded from Pithecellobium dulce (Roxb.) Benth. seeds. The physicochemical properties were analyzed in terms of swelling power, solubility, pH and water holding capacity. Micromeretic studies proved the polysaccharide may be used a potential pharmaceutical adjuvant. The polysaccharide was characterized by FT-IR, SEM, TGA and NMR techniques. Methylation analysis confirmed that the polysaccharide is composed of Arabinose (Araf) units. The chemical shifts of anomeric proton region were found in the region of 4.4-5.5ppm. Thermogravimetric analysis showed that PDP polysaccharide was thermally stable. The in vitro antioxidant capacities of the polysaccharide were investigated in terms of scavenging of hydroxyl radicals, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals, hydrogen peroxide (H2O2) and reducing power assay. The polysaccharide fractions showed activity in a concentration dependent manner which was comparable to the standard, ascorbic acid.
authors.affiliation: Department of Pharmaceutical Sciences and Technology, BIT Mesra, Ranchi, Jharkhand, 835215, India.
authors.forename: S
authors.initials: S
authors.lastname: Bagchi
authors.affiliation: Department of Pharmaceutical Sciences and Technology, BIT Mesra, Ranchi, Jharkhand, 835215, India. Electronic address: jayarampharm@gmail.com.
authors.forename: K Jayaram
authors.initials: KJ
authors.lastname: Kumar
mesh_terms: D007887:Fabaceae / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry*; D012639:Seeds / Q000378:metabolism; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D011134:Polysaccharides; D014867:Water; D017665:Hydroxyl Radical
keywords: Antioxidant; Pithecellobium dulce (Roxb.) Benth.; Water soluble PDP polysaccharides