title: Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and computer-assisted sperm analysis (CASA) semen variables.
abstract: The present study is the first to quantify leptin in seminal plasma of buffalo and investigate its relationship with seminal attributes. Ten ejaculates each from 10 Murrah buffalo bulls were collected. Semen quality variables such as semen volume, sperm concentration, sperm abnormalities, membrane integrity, antioxidant enzyme activities (superoxide dismutase, catalase and total antioxidant capacity), malondialdehyde (MDA) concentration, as well as sperm kinetics and motility variables were evaluated. The leptin concentration in serum and seminal plasma were estimated by the ELISA method. Bulls were classified in two groups on the basis of sperm concentration with Group I having >800 million sperm/mL and Group II <500 million sperm/mL. Greater (P<0.05) mean sperm abnormalities, seminal leptin concentrations and MDA concentrations were recorded in Group II than Group I. The seminal leptin was positively correlated with sperm abnormalities and MDA concentration while being negatively correlated with sperm concentration, but there was no correlation with sperm kinetic and motility variables, sperm membrane integrity and seminal plasma antioxidant enzyme activity. Thus, the data suggest that seminal leptin has a role in spermatogenesis and can be used as a marker for spermatogenesis to predict the capacity of buffalo bulls for semen production.
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India. Electronic address: drpradeepkrvet@gmail.com.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Saini
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: M H
authors.initials: MH
authors.lastname: Jan
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India; Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
authors.forename: Dheer Singh
authors.initials: DS
authors.lastname: Swami
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D002020:Buffaloes / Q000378:metabolism; D015925:Cryopreservation / Q000662:veterinary; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D020738:Leptin / Q000032:analysis* / Q000378:metabolism; D008297:Male; D012661:Semen / Q000737:chemistry* / Q000378:metabolism; D055101:Semen Analysis / Q000379:methods* / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa
chemical_list: D000975:Antioxidants; D020738:Leptin
keywords: Buffalo; CASA; Cryopreservation; Leptin; Semen quality