title: Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp.
abstract: The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroAPantoea sp is a class I AroA enzyme. When expressed in Escherichia coli, it conveyed high tolerance to glyphosate. AroAPantoea sp may be used to generate transgenic glyphosate-tolerant plants.
authors.affiliation: Plant Science Department, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.affiliation: Plant Science Department, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Cao
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020636:Pantoea / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012765:Shikimic Acid / Q000378:metabolism; D012988:Soil Microbiology; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D001426:Bacterial Proteins; D005998:Glycine; D006540:Herbicides; D011994:Recombinant Proteins; D012765:Shikimic Acid; D000097797:Glyphosate