title: Treatment of large bone defects with a novel biological transport disc in non-vascular transport distraction osteogenesis.
abstract: The aim of this study was to investigate a potential novel biological transport disc that avoids secondary injury to the body and facilitates bone healing. Twenty-seven dogs were divided randomly into three groups: group A were treated with human bone morphogenetic protein 2 (BMP-2) modified bone mesenchymal stem cell (BMSC) sheets combined with freeze-dried bone allograft as biological transport disc; group B were treated with BMSC sheets combined with freeze-dried bone allograft as transport disc (control); and group C were treated with direct extension only (blank). There were nine dogs in each group. Non-vascular transport distraction osteogenesis was performed in groups A and B to repair the mandibular bone defects, and in group C only mandibular truncation surgery was performed. The regeneration of bone was evaluated through X-ray, haematoxylin and eosin assay, and immunohistochemistry. After 2, 4, and 8 weeks of distraction, new bone density values in group A were 49.00±1.16, 66.63±2.62, and 72.78±2.67, respectively, and these were significantly different to values in groups B (P=0.0005, P=0.0004, P=0.0012) and C (P<0.0005, P=0.0001, P=0.0003). The average grey value for BMP-2 expression in group A after 4 weeks of distraction was 195.63±4.45, which was significantly different when compared to groups B (P=0.0022) and C (P=0.0006). This novel biological transport disc represents an effective non-secondary injury method to enhance new bone formation in non-vascular transport distraction osteogenesis.
authors.affiliation: The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zeng
authors.affiliation: College of Stomatology, Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: P
authors.initials: P
authors.lastname: Guo
authors.affiliation: College of Stomatology, Guangxi Medical University, Nanning, Guangxi, China. Electronic address: james249540408@hotmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Zhou
authors.affiliation: College of Stomatology, Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Q T
authors.initials: QT
authors.lastname: Xie
authors.affiliation: College of Stomatology, Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: F C
authors.initials: FC
authors.lastname: Liao
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004285:Dogs; D005612:Freeze Drying; D007150:Immunohistochemistry; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D019857:Osteogenesis, Distraction / Q000379:methods*; D011897:Random Allocation
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2
keywords: biological transport disc; bone marrow mesenchymal stem cells; cell sheet; freeze-dried bone allograft; human bone morphogenetic protein 2; transport distraction osteogenesis