title: Development of a Novel Functional Monoclonal Antibody to Human CD275: Characterization and Biological Activity.
abstract: CD275 (B7-H2, ICOSL), a co-stimulatory molecule of the B7 superfamily, plays a critical role in immune response. In this report, a novel mouse anti-human CD275 monoclonal antibody (MAb) was prepared using hybridoma technology, and immunological characteristics of the MAb were determined. The results showed that the MAb (clone 13D11) was IgG2(κ) and bound specifically to human CD275. By mutual competition, we found that the antibody recognized different epitopes of CD275 antigen compared with commercial antibodies and could block ICOS-CD275 interaction. Crosslinking of CD275 with MAb 13D11 markedly blocked ICOS positive signal and inhibited T cell proliferation and cytokine production. In addition, the 13D11 MAb was suitable for indirect ELISA detection. Thus, the MAb against human CD275 with high specificity and different activity would be useful for further study of this molecule.
authors.affiliation: 1 Jiangsu Province Key Laboratory of Stem Cell Research, Soochow University , Suzhou, China .
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Hu
authors.affiliation: 2 Jiangsu Institute of Clinical Immunology, the First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Cuiping
authors.initials: C
authors.lastname: Liu
authors.affiliation: 1 Jiangsu Province Key Laboratory of Stem Cell Research, Soochow University , Suzhou, China .
authors.forename: Jingnan
authors.initials: J
authors.lastname: An
authors.affiliation: 2 Jiangsu Institute of Clinical Immunology, the First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: 1 Jiangsu Province Key Laboratory of Stem Cell Research, Soochow University , Suzhou, China .
authors.forename: Yumin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: 2 Jiangsu Institute of Clinical Immunology, the First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Juean
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 2 Jiangsu Institute of Clinical Immunology, the First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: 1 Jiangsu Province Key Laboratory of Stem Cell Research, Soochow University , Suzhou, China .|2 Jiangsu Institute of Clinical Immunology, the First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D000918:Antibody Specificity; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; C498514:ICOSLG protein, human; C400230:Icosl protein, mouse; D060945:Inducible T-Cell Co-Stimulator Ligand