title: Purification and Structural Analysis of Desmoplakin.
abstract: Desmoplakin (DP) is an obligate component of desmosomes, where it links the desmosomal cadherin/plakoglobin/plakophilin assembly to intermediate filaments. DP contains a large amino-terminal domain (DPNT) that binds to the cadherin/plakoglobin/plakophilin complex, a central coiled-coil domain that dimerizes the molecule, and a C-terminal domain (DPCT) that binds to intermediate filaments. DPNT contains a plakin domain, comprising a set of spectrin-like repeats. DPCT contains three plakin repeat domains, each formed by 4.5 repeats of a sequence motif known as a plakin repeat that bind to intermediate filaments. Here, we review purification, biochemical characterization, and structural analysis of the DPNT plakin domain and the DPCT plakin repeat domains.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA; Department of Molecular & Cellular Physiology, Stanford University School of Medicine, Stanford, California, USA. Electronic address: bill.weis@stanford.edu.
authors.forename: William I
authors.initials: WI
authors.lastname: Weis
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D051180:Desmoplakins / Q000737:chemistry / Q000302:isolation & purification*; D004926:Escherichia coli; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: C495294:DSP protein, human; D051180:Desmoplakins
keywords: Desmoplakin; Desmosome; Intermediate filament; Plakin domain; Plakin repeat; Plakoglobin; Plakophilin; Spectrin repeat; Vimentin