title: Cloning of genes related to aliphatic glucosinolate metabolism and the mechanism of sulforaphane accumulation in broccoli sprouts under jasmonic acid treatment.
abstract: BACKGROUND
  Cytochrome P450 79F1 (CYP79F1), cytochrome P450 83A1 (CYP83A1), UDP-glucosyltransferase 74B1 (UGT74B1), sulfotransferase 18 (ST5b) and flavin-containing monooxygenase GS-OX1 (FMOGS - OX1 ) are important enzymes in aliphatic glucosinolate biosynthesis. In this study, their full-length cDNA in broccoli was firstly cloned, then the mechanism of sulforaphane accumulation under jasmonic acid (JA) treatment was investigated.
  
  
  RESULTS
  The full-length cDNA of CYP79F1, CYP83A1, UGT74B1, ST5b and FMOGS - OX1 comprised 1980, 1652, 1592, 1378 and 1623 bp respectively. The increase in aliphatic glucosinolate accumulation in broccoli sprouts treated with JA was associated with elevated expression of genes in the aliphatic glucosinolate biosynthetic pathway. Application of 100 µmol L(-1) JA increased myrosinase (MYR) activity but did not affect epithiospecifier protein (ESP) activity in broccoli sprouts, which was supported by the expression of MYR and ESP. Sulforaphane formation in 7-day-old sprouts treated with 100 µmol L(-1) JA was 3.36 and 1.30 times that in the control and 300 µmol L(-1) JA treatment respectively.
  
  
  CONCLUSIONS
  JA enhanced the accumulation of aliphatic glucosinolates in broccoli sprouts via up-regulation of related gene expression. Broccoli sprouts treated with 100 µmol L(-1) JA showed higher sulforphane formation than those treated with 300 µmol L(-1) JA owing to the higher glucoraphanin content and myrosinase activity under 100 µmol L(-1) JA treatment. © 2016 Society of Chemical Industry.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.|College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Runqiang
authors.initials: R
authors.lastname: Yang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Gu
mesh_terms: D016588:Anticarcinogenic Agents / Q000378:metabolism*; D001937:Brassica / Q000737:chemistry / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002681:China; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004790:Enzyme Induction; D055951:Functional Food / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017879:Isothiocyanates / Q000378:metabolism*; D008970:Molecular Weight; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013454:Sulfoxides
chemical_list: D016588:Anticarcinogenic Agents; D003517:Cyclopentanes; D005961:Glucosinolates; D007527:Isoenzymes; D017879:Isothiocyanates; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011994:Recombinant Proteins; D013454:Sulfoxides; C011006:jasmonic acid; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; C089817:cytochrome P450TYR; D005964:Glucosyltransferases; D015238:Sulfotransferases; D006026:Glycoside Hydrolases; C022739:thioglucosidase; C016766:sulforaphane
keywords: broccoli sprouts; gene expression; glucoraphanin; jasmonic acid; sulforaphane