title: Thermogladius calderaegen. nov., sp. nov., an anaerobic, hyperthermophilic crenarchaeote from a Kamchatka hot spring.
abstract: An obligately anaerobic, hyperthermophilic, organoheterotrophic archaeon, strain 1633T, was isolated from a terrestrial hot spring of the Uzon Caldera (Kamchatka Peninsula, Russia). Cells were regular cocci, 0.5-0.9 μm in diameter, with one flagellum. The temperature range for growth was 80-95 °C, with an optimum at 84 °C. Strain 1633Tgrew on yeast extract, beef extract, peptone, cellulose and cellobiose. No growth was detected on other sugars or carbohydrates, organic acids, or under autotrophic conditions. The only detected growth products were CO2, acetate, and H2. The growth rate was stimulated by elemental sulfur, which was reduced to hydrogen sulfide. Thein silico-calculated G+C content of the genomic DNA of strain 1633Twas 55.64 mol%. 16S rRNA gene sequence analysis placed strain 1633Ttogether with the non-validly published 'Thermogladius shockii' strain WB1 in a separate genus-level cluster within the familyDesulfurococcaceae. Average nucleotide identity (ANI) results revealed 75.72 % identity between strain 1633Tand 'Thermogladius shockii' WB1. Based on these results we propose a novel genus and species with the nameThermogladius calderaegen. nov., sp. nov. The type strain of the type species is 1633T( = DSM 22663T= VKM B-2946T).
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS,33/2 Leninsky prospect, 119071, Moscow,Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Kochetkova
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS,33/2 Leninsky prospect, 119071, Moscow,Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Kublanov
authors.affiliation: Immanuel Kant Baltic Federal University,Botkina str. 3, 236016, Kaliningrad,Russia.
authors.forename: Stepan V
authors.initials: SV
authors.lastname: Toshchakov
authors.affiliation: Department of Earth and Planetary Sciences, Northwestern University,Evanston, IL,USA.
authors.forename: Magdalena R
authors.initials: MR
authors.lastname: Osburn
authors.affiliation: Gubkin Russian State University of Oil and Gas,Leninskiy Prospect 65, 119991, Moscow,Russia.
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Novikov
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS,33/2 Leninsky prospect, 119071, Moscow,Russia.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS,33/2 Leninsky prospect, 119071, Moscow,Russia.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Perevalova