title: MicroRNA heterogeneity in endometrial cancer cell lines revealed by deep sequencing.
abstract: The aim of the present study was to obtain comprehensive microRNA (miRNA) profiles of type I [Ishikawa (ISK)] and type II (HEC-1B) human endometrial adenocarcinoma cell lines, utilizing the latest high-throughput sequencing techniques. RNA was extracted from ISK and HEC-1B cell lines. Sequencing results were obtained from a next-generation sequencing platform. Using the miRBase database and a series of software pipelines, miRNA expression was analyzed in the ISK and HEC-1B cell lines. It was revealed that the type and quantity of miRNAs in the two cell types varied significantly; 34 miRNAs were upregulated and 105 miRNAs were downregulated in HEC-1B cells compared with those of ISK cells. Furthermore, it was observed that the expression pattern of the miRNA (miR)-17-92 cluster differed between the two cell types, and the expression levels of the miR-200 family in ISK cells were markedly increased compared with those of HEC-1B cells. The present study therefore identified potential novel biomarkers, which may be useful in the differentiation between type I and type II endometrial cancer, and also revealed miRNA alterations that may be associated with endometrial cancer and its underlying pathogenic mechanisms.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu 210096, P.R. China.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Surgery, Fengxian Central Hospital, Shanghai 201400, P.R. China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Fengxian Central Hospital, Shanghai 201400, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu 210096, P.R. China; Research Center for Learning Science, Southeast University, Nanjing, Jiangsu 210096, P.R. China.
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
keywords: HEC-1B; Ishikawa; deep sequencing; endometrial cancer; microRNA