title: Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India.
abstract: The present study evaluated the ability of MIRU-VNTRs to discriminate Mycobacterium tuberculosis (MTB) clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping (discriminatory index of 0.9113) differentiated the strains into 68 distinct patterns, the biggest cluster being SIT11/48 SIT11 (n=48). The present study shows that 12-loci MIRUs and 3 ETRs allowed an efficient discrimination of these isolates (discriminatory indexes of 0.7819 and 0.5523, respectively).
authors.affiliation: Department of Biotechnology, Christ University, Hosur Road, Bangalore 560 029, Karnataka, India.
authors.forename: Biljo V
authors.initials: BV
authors.lastname: Joseph
authors.affiliation: Mycobacteria Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, Thiruvananthapuram 695 014, Kerala, India.
authors.forename: Smitha
authors.initials: S
authors.lastname: Soman
authors.affiliation: WHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, Abymes, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Hill
authors.affiliation: Mycobacteria Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, Thiruvananthapuram 695 014, Kerala, India.
authors.forename: R Ajay
authors.initials: RA
authors.lastname: Kumar
authors.affiliation: WHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, Abymes, France.
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
authors.affiliation: Mycobacteria Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, Thiruvananthapuram 695 014, Kerala, India. Electronic address: smundayoor@rgcb.res.in.
authors.forename: Sathish
authors.initials: S
authors.lastname: Mundayoor
keywords: IS6110-RFLP; India; Kerala; MIRU-VNTR; Mycobacterium tuberculosis; Spoligotyping