title: Evaluation of Selected Immunomodulatory Glycoproteins as an Adjunct to Cancer Immunotherapy.
abstract: Polysaccharopeptide (PSP), from Coriolus versicolor, has been used widely as an adjuvant to chemotherapy with demonstrated anti-tumor and broad immunomodulating effects. While PSP's mechanism of action still remains unknown, its enhanced immunomodulatory potential with acacia gum is of great interest. Acacia gum, which also contains polysaccharides and glycoproteins, has been demonstrated to be immunopotentiating. To elucidate whether PSP directly activates T-cell-dependent B-cell responses in vivo, we used a well-established hapten carrier system (Nitrophenyl-chicken gamma globulin (NP-CGG)). 6-week C57BL/6 male mice were immunised with 50 μg of NP25-CGG alum precipitate intraperitoneally. Mice were gavaged daily with 50 mg/kg PSP in a vehicle containing acacia gum and sacrificed at days 0, 4, 7, 10, 14 and 21. ELISA was used to measure the total and relative hapten-specific anti-NP IgA, IgM and IgG titre levels compared to the controls. It was found that PSP, combined with acacia gum, significantly increased total IgG titre levels at day 4 (P< 0.05), decreased IgM titre levels at days 4 and 21 (P< 0.05) with no alterations observed in the IgA or IgE titre levels at any of the time points measured. Our results suggest that while PSP combined with acacia gum appears to exert weak immunological effects through specific T-cell dependent B-cell responses, they are likely to be broad and non-specific which supports the current literature on PSP. We report for the first time the application of a well-established hapten-carrier system that can be used to characterise and delineate specific T-cell dependent B-cell responses of potential immunomodulatory glycoprotein-based herbal medicines combinations in vivo.
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.
authors.forename: Bhagwant Kaur
authors.initials: BK
authors.lastname: Sekhon
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.
authors.forename: Rebecca Heidi
authors.initials: RH
authors.lastname: Roubin
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.
authors.forename: Parimala B
authors.initials: PB
authors.lastname: Devi
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.|School of Medical Science, University of Western Sydney, Campbelltown, New South Wales, 2560, Australia.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Nammi
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.
authors.forename: Kei
authors.initials: K
authors.lastname: Fan
authors.affiliation: Faculty of Pharmacy, The University of Sydney, New South Wales, 2006, Australia.|School of Medical Sciences and Health Innovations Research Institute (HIRi), RMIT University, Victoria, 3000, Australia.
authors.forename: Daniel Man-yuen
authors.initials: DM
authors.lastname: Sze
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D006170:Gum Arabic / Q000494:pharmacology*; D006241:Haptens / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007167:Immunotherapy / Q000379:methods; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000628:therapy; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates / Q000276:immunology; D011509:Proteoglycans / Q000276:immunology* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D055454:Trametes / Q000276:immunology; D055545:gamma-Globins / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D006241:Haptens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009596:Nitrophenols; D010648:Phenylacetates; D011509:Proteoglycans; D055545:gamma-Globins; C093216:polysaccharide peptide; C026242:4-hydroxy-5-nitrophenyl acetic acid; D006170:Gum Arabic