title: Fast Detection of Phenolic Compounds in Extracts of Easter Pears (Pyrus communis) from the Atacama Desert by Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-Q/Orbitrap/MS/MS).
abstract: A small Chilean variety of pears growing in the town of Toconao, an oasis located at the northeastern edge of the Salar de Atacama, northern Chile, was studied by means of modern PDA and high resolution mass spectral data (UHPLC-PDA-HESI-orbitrap-MS/MS). In addition, the antioxidant features of the fruits were compared with the varieties Packhman's Triumph and Abate Fetel and correlated with the presence of phenolic compounds. The non-pigmented phenolics were fingerprinted and related to the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics and flavonoids measured by spectroscopic methods. The machine allowed a fast separation of 15 min employing a flow rate of 1 mL per minute and could accurately identify 25 compounds, including several isorhamnetin derivatives and phenolic acids, present in the peel and pulps of this Chilean variety for the first time. The compounds were monitored using a wavelength range of 210-800 nm. The native small Chilean pear showed the highest antioxidant activity measured as the bleaching of the DPPH radical, the ferric reducing antioxidant power and superoxide anion scavenging activity (8.61 ± 0.65 μg/mL, 712.63 ± 12.12 micromols trolox equivalents (μmol/TE)/100 g FW, and 82.89% ± 2.52% at 100 μg/mL, respectively).
authors.affiliation: Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile. mario.simirgiotis@uantof.cl.
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Simirgiotis
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Arturo Prat, Casilla 121, Iquique 1110939, Chile. elquispe@unap.cl.
authors.forename: Cristina
authors.initials: C
authors.lastname: Quispe
authors.affiliation: Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile. jorge.borquez@uantof.cl.
authors.forename: Jorge
authors.initials: J
authors.lastname: Bórquez
authors.affiliation: Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile. areche@uchile.cl.
authors.forename: Carlos
authors.initials: C
authors.lastname: Areche
authors.affiliation: Departamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar 2520000, Chile. bsepulveda@uc.cl.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sepúlveda
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D002677:Chile; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003889:Desert Climate; D005419:Flavonoids / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D031989:Pyrus / Q000737:chemistry* / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C047368:3-methylquercetin; D013481:Superoxides; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Chilean pears; Orbitrap (OT); Pera de Pascua; UHPLC-MS; antioxidant capacity; flavonoids; phenolics; proanthocyanidins