title: Unusual poly(3-hydroxyalkanoate) (PHA) biosynthesis behavior of Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002 isolated from palm oil mill effluent.
abstract: Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002, isolated from palm oil mill effluent, accumulated poly(3-hydroxyalkanoates) (PHAs) when grown on aliphatic fatty acids, sugars, and glycerol. The substrates were supplied at 20:1 C/N mole ratio. Among C-even n-alkanoic acids, myristic acid gave the highest PHA content 26 and 28 wt% in P. putida and D. tsuruhatensis, respectively. Among C-odd n-alkanoic acids, undecanoic gave the highest PHA content at 40 wt% in P. putida and 46 wt% in D. tsuruhatensis on pentadecanoic acid. Sugar and glycerol gave <10 wt% of PHA content for both bacteria. Interestingly, D. tsuruhatensis accumulated both short- and medium-chain length PHA when supplied with n-alkanoic acids ranging from octanoic to lauric, sucrose, and glycerol with 3-hydroxybutyrate as the major monomer unit. In P. putida, the major hydroxyalkanoates unit was 3-hydroxyoctanoate and 3-hydroxydecanoate when grown on C-even acids. Conversely, 3-hydroxyheptanoate, 3-hydrxoynonanoate, and 3-hydroxyundecanoate were accumulated with C-odd acids. Weight-averaged molecular weight (Mw) was in the range of 53-81 kDa and 107-415 kDa for P. putida and D. tsuruhatensis, respectively. Calorimetric analyses indicated that both bacteria synthesized semicrystalline polymer with good thermal stability with degradation temperature (Td) ranging from 178 to 282 °C.
authors.affiliation: Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Mohd Rafais Mohd
authors.initials: MRM
authors.lastname: Razaif-Mazinah
authors.affiliation: Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Siti Nor Syairah
authors.initials: SNS
authors.lastname: Anis
authors.affiliation: Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Hazwani Izzati
authors.initials: HI
authors.lastname: Harun
authors.affiliation: Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Khairunnisa Abdul
authors.initials: KA
authors.lastname: Rashid
authors.affiliation: Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.|Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Mohamad Suffian Mohamad
authors.initials: MSM
authors.lastname: Annuar
mesh_terms: D002210:Caprylates / Q000737:chemistry; D002244:Carbon; D042742:Delftia / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D008970:Molecular Weight; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002210:Caprylates; D005227:Fatty Acids; D010938:Plant Oils; D054813:Polyhydroxyalkanoates; D000073878:Palm Oil; D002244:Carbon; C101940:3-hydroxyoctanoic acid; D005990:Glycerol
keywords: Delftia tsuruhatensis; Pseudomonas putida; odd and even carbon sources; scl-co-mcl PHA; sugars