title: The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.
abstract: The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl β-D-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance.
authors.affiliation: United States Department of Agriculture, Renewable Product Technology unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA. Siqing.liu@ars.usda.gov.
authors.forename: Siqing
authors.initials: S
authors.lastname: Liu
authors.affiliation: United States Department of Agriculture, Renewable Product Technology unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Skory
authors.affiliation: United States Department of Agriculture, Bioenergy Research unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.
authors.forename: Nasib
authors.initials: N
authors.lastname: Qureshi
authors.affiliation: United States Department of Agriculture, Renewable Product Technology unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Hughes
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000235:genetics*; D021381:Protein Transport / Q000187:drug effects; D000069816:SEC Translocation Channels / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D000069816:SEC Translocation Channels; D000431:Ethanol
keywords: Escherichia coli; Ethanol tolerance; Lactobacillus buchneri; Membrane protein; yajC