title: Description of Endozoicomonas arenosclerae sp. nov. using a genomic taxonomy approach.
abstract: The taxonomic position of strains Ab112(T) (CBAS 572(T)) and Ab227_MC (CBAS 573) was evaluated by means of genomic taxonomy. These isolates represent the dominant flora cultured from the healthy marine sponge Arenosclera brasiliensis, endemic to Rio de Janeiro. Strains CBAS 572(T) and CBAS 573 shared >98 % 16S rRNA sequence identity with Endozoicomonas numazuensis and Endozoicomonas montiporae. In silico DNA-DNA Hybridization, i.e. genome-to-genome distance (GGD), amino acid identity (AAI) and average nucleotide identity (ANI) further showed that these strains had <70 %, at maximum 71.1 and 78 % of identity, respectively, to their closest neighbours E. numazuensis and E. montiporae. The DNA G+C content of CBAS 572(T) and CBAS 573 were 47.6 and 47.7 mol%, respectively. Phenotypic and chemotaxonomic features also allowed a separation from the type strains of their phylogenetic neighbours. Useful phenotypic features for discriminating CBAS 572(T) and CBAS 573 from E. numazuensis and E. montiporae species include C8 esterase, N-acetyl-β-glucosaminidase, citric acid, uridine and siderophore. The species Endozoicomonas arenosclerae sp. nov. is proposed to harbour the new isolates. The type strain is CBAS 572(T) (=Ab112(T)).
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Luciana R
authors.initials: LR
authors.lastname: Appolinario
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Diogo A
authors.initials: DA
authors.lastname: Tschoeke
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Cintia P J
authors.initials: CP
authors.lastname: Rua
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Tainá
authors.initials: T
authors.lastname: Venas
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Mariana E
authors.initials: ME
authors.lastname: Campeão
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Gilda R S
authors.initials: GR
authors.lastname: Amaral
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Leomil
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Louisi
authors.initials: L
authors.lastname: de Oliveira
authors.affiliation: Instituto Oswaldo Cruz (IOC), FIOCRUZ, Rio De Janeiro, RJ, Brazil.
authors.forename: Verônica Viana
authors.initials: VV
authors.lastname: Vieira
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Koko
authors.initials: K
authors.lastname: Otsuki
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.|Laboratory for Microbiology, Ghent University, Ghent, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil.|SAGE-COPPE, Federal University of Rio de Janeiro, Rio De Janeiro, RJ, Brazil.
authors.forename: Fabiano L
authors.initials: FL
authors.lastname: Thompson
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, Brazil. thompsoncristiane@gmail.com.
authors.forename: Cristiane C
authors.initials: CC
authors.lastname: Thompson
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D058893:DNA Barcoding, Taxonomic; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D056726:Genetic Association Studies; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Comparative genomics; Endozoicomonas new species; Genomes; Genomic taxonomy