title: High carotenoid bioaccessibility through linseed oil nanoemulsions with enhanced physical and oxidative stability.
abstract: Carotenoid (astaxanthin or lycopene) emulsions obtained by high pressure homogenization were investigated for their physical, oxidative and storage stability and biological fate on an in vitro digestion model of bioaccessibility. Emulsion stability evaluated at various processing environments (20-50°C, 2-10 pH, 0-500 mM NaCl, and 0-35 days storage at 25°C) depended on carotenoid and homogenization pressures (5, 10, 100 MPa). Trolox increased the oxidative stability of nanoemulsions (100 MPa) and acted synergistically with BHT in increasing the stability of lycopene nanoemulsion. Intestinal digestibility depended on homogenization pressures with the fastest release and lower amount of free fatty acids observed at 100 MPa. Carotenoid nanoemulsions (100 MPa) were partially (66%) digested and highly bioaccessible (>70%). Therefore, nanoemulsions provide an effective and stable system for efficient astaxanthin or lycopene delivery and bioavailability in foods, beverages, nutraceuticals and/or other agriproducts.
authors.affiliation: Master of Engineering Sciences with Specialization in Biotechnology, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Daniela
authors.initials: D
authors.lastname: Sotomayor-Gerding
authors.affiliation: National Bioproducts and Bioprocesses Program, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, BC V0H 1Z0, Canada(1).
authors.forename: B Dave
authors.initials: BD
authors.lastname: Oomah
authors.affiliation: Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Francisca
authors.initials: F
authors.lastname: Acevedo
authors.affiliation: Master of Engineering Sciences with Specialization in Biotechnology, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Morales
authors.affiliation: Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Mariela
authors.initials: M
authors.lastname: Bustamante
authors.affiliation: Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Centre for Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Carolina
authors.initials: C
authors.lastname: Shene
authors.affiliation: Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: monica.rubilar@ufrontera.cl.
authors.forename: Mónica
authors.initials: M
authors.lastname: Rubilar
mesh_terms: D001682:Biological Availability; D002338:Carotenoids / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry*; D008043:Linseed Oil / Q000737:chemistry*; D000077276:Lycopene
chemical_list: D004655:Emulsions; D002338:Carotenoids; D008043:Linseed Oil; D000077276:Lycopene
keywords: Antioxidants; Bioaccessibility; Carotenoids; Linseed oil; Nanoemulsions