title: Discriminative potential of some PCR-based and biochemical methods at Scedosporium strains.
abstract: Three innovative PCR-based methods (fluorescent-ITS, fluorescent-CBH and ITS-PCR DGGE) were tested using a reference set of nine strains of Scedosporium from the CBS fungal collection. Cellulolytic, lipolytic and proteolytic potential and the ability to dissolve CaCO3 of the strains were evaluated in vitro by means of agar assays. f-ITS profiles almost recognized main species, although included "Pseudallescheria" ellipsoidea and the Scedosporium boydii CBS 117432 and CBS 120157 in the same cluster. All strains successfully produced DNA polymorphisms by f-CBH amplification which divided them into three different groups. The DGGE approach separated the strains studied into other five clusters which in some case were not matching with species. Strains tested were monomorphic in possessing strong proteolytic and lipolytic activities. The comparison of the three PCR-based genotyping approaches, together with biodegradation ability screening, displayed an intraspecies variability in S. boydii, interfering with unambiguous species delimitation.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Kraková
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia.
authors.forename: Domenico
authors.initials: D
authors.lastname: Pangallo
authors.affiliation: Medical Faculty, Slovak Medical University, Limbová 12, 833 03 Bratislava, Slovakia. Electronic address: elena.pieckova@szu.sk.
authors.forename: Elena
authors.initials: E
authors.lastname: Piecková
authors.affiliation: Medical Faculty, Slovak Medical University, Limbová 12, 833 03 Bratislava, Slovakia.
authors.forename: Mária
authors.initials: M
authors.lastname: Majorošová
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D005838:Genotype; D016533:Mycological Typing Techniques / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D021681:Scedosporium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal
keywords: Fluorescent-CBH; Fluorescent-ITS; Hydrolytic activity; ITS–PCR DGGE