title: Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with Eimeria tenella.
abstract: The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang 471003, China.
authors.forename: Bian-Hua
authors.initials: BH
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang 471003, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Liu
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang 471003, China.
authors.forename: Fu-Wei
authors.initials: FW
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang 471003, China.
authors.forename: Er-Jie
authors.initials: EJ
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang 471003, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Wang
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000033:anatomy & histology / Q000469:parasitology*; D002645:Chickens; D003048:Coccidiosis / Q000188:drug therapy / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D003049:Coccidiostats / Q000008:administration & dosage / Q000009:adverse effects*; D016786:Eimeria tenella / Q000502:physiology*; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009570:Nitriles / Q000008:administration & dosage / Q000009:adverse effects*; D011201:Poultry Diseases / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D014227:Triazines / Q000008:administration & dosage / Q000009:adverse effects*
chemical_list: D003049:Coccidiostats; D007071:Immunoglobulin A, Secretory; D009570:Nitriles; D014227:Triazines; C057884:diclazuril
keywords: Eimeria tenella; bursa of Fabricius (BF); chicken; diclazuril; secretory immunoglobulin A (SIgA); ultrastructure