title: Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins.
abstract: Most proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls the cellular functions that involve protein complexes. Although several artificial dimerization systems have already been used for numerous applications in biomedical research, cellular protein complexes form not only simple dimers but also larger oligomers. In this study, we showed that fusion proteins comprising the induced heterodimer formation proteins FRB and FKBP formed various oligomers upon addition of rapamycin. By adjusting the configuration of fusion proteins, we succeeded in generating an inducible tetramer formation system. Proteins of interest also formed tetramers by fusing to the inducible tetramer formation system, which exhibits its utility in a broad range of biological applications.
authors.affiliation: Frontier Research Core for Life Sciences, University of Toyama, 3190 Gofuku, Toyama-shi, Toyama 930-8555, Japan. Electronic address: inobe@ctg.u-toyama.ac.jp.
authors.forename: Tomonao
authors.initials: T
authors.lastname: Inobe
authors.affiliation: Laboratory of Structural Neuropathology, Doshisha University Graduate School of Brain Science, 1-3 Tatara Miyakodani, Kyotanabe-shi, Kyoto 610-0394, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Nukina
mesh_terms: D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D000072417:Protein Domains / Q000187:drug effects; D055503:Protein Multimerization / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D011993:Recombinant Fusion Proteins; D058570:TOR Serine-Threonine Kinases; D022021:Tacrolimus Binding Proteins; D020123:Sirolimus
keywords: Chemically-induced oligomerization; FKBP; FRB; Oligomer; Protein engineering; Rapamycin