title: [Expression pattern of membrane type-1 matrix metalloproteinase in primary breast carcinomas].
abstract: OBJECTIVE
  To examine the expression pattern of membrane type-1 matrix metalloproteinase (MT1-MMP) in breast carcinomas.
  
  
  METHODS
  Forty-three breast cancer tissues were collected and examined for MT1-MMP protein and mRNA expressions using immunohistochemistry, semi-quantitative RT-PCR and in situ hybridization.
  
  
  RESULTS
  Immunohistochemistry of the breast cancer specimens showed MT1-MMP immunoreactivity on the cancer cell membrane. MT1-MMP mRNA was located in the stromal cells surrounding the breast cancer nest as shown by in situ hybridization. MT1-MMP mRNA expression was detected in all of the carcinomas, but its level was significantly lower in immunohistochemically negative specimens than in positive ones (0.547=0.0886 vs 0.759=0.0802, Plt;0.01).
  
  
  CONCLUSIONS
  MT1-MMP is very likely produced by stromal cells surrounding the breast cancer nest and anchored on the cell membrane after activation.
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: ygy531@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Yao
authors.forename: Lujia
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Hu
authors.forename: Wenji
authors.initials: W
authors.lastname: Li
authors.forename: Changsheng
authors.initials: C
authors.lastname: Ye
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14