title: Deferoxamine prevents cerebral glutathione and vitamin E depletions in asphyxiated neonatal rats: role of body temperature.
abstract: Hypoxic-ischaemic brain injury involves increased oxidative stress. In asphyxiated newborns iron deposited in the brain catalyses formation of reactive oxygen species. Glutathione (GSH) and vitamin E are key factors protecting cells against such agents. Our previous investigation has demonstrated that newborn rats, showing physiological low body temperature as well as their hyperthermic counterparts injected with deferoxamine (DF) are protected against iron-mediated, delayed neurotoxicity of perinatal asphyxia. Therefore, we decided to study the effects of body temperature and DF on the antioxidant status of the brain in rats exposed neonatally to critical anoxia. Two-day-old newborn rats were exposed to anoxia in 100% nitrogen atmosphere for 10 min. Rectal temperature was kept at 33 °C (physiological to rat neonates), or elevated to the level typical of healthy adult rats (37 °C), or of febrile adult rats (39 °C). Half of the rats exposed to anoxia under extremely hyperthermic conditions (39 °C) were injected with DF. Cerebral concentrations of malondialdehyde (MDA, lipid peroxidation marker) and the levels of GSH and vitamin E were determined post-mortem, (1) immediately after anoxia, (2) 3 days, (3) 7 days, and (4) 2 weeks after anoxia. There were no post-anoxic changes in MDA, GSH and vitamin E concentrations in newborn rats kept at body temperature of 33 °C. In contrast, perinatal anoxia at elevated body temperatures intensified oxidative stress and depleted the antioxidant pool in a temperature-dependent manner. Both the depletion of antioxidants and lipid peroxidation were prevented by post-anoxic DF injection. The data support the idea that hyperthermia may extend perinatal anoxia-induced brain lesions.
authors.affiliation: a N. Copernicus University , Department of Animal Physiology, Faculty of Biology and Environmental Protection , Toruń , Poland and.
authors.forename: Hanna
authors.initials: H
authors.lastname: Kletkiewicz
authors.affiliation: a N. Copernicus University , Department of Animal Physiology, Faculty of Biology and Environmental Protection , Toruń , Poland and.
authors.forename: Anna
authors.initials: A
authors.lastname: Nowakowska
authors.affiliation: a N. Copernicus University , Department of Animal Physiology, Faculty of Biology and Environmental Protection , Toruń , Poland and.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Siejka
authors.affiliation: b N. Copernicus University , Department of Medical Biology, Collegium Medicum , Bydgoszcz , Poland.
authors.forename: Celestyna
authors.initials: C
authors.lastname: Mila-Kierzenkowska
authors.affiliation: b N. Copernicus University , Department of Medical Biology, Collegium Medicum , Bydgoszcz , Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Woźniak
authors.affiliation: a N. Copernicus University , Department of Animal Physiology, Faculty of Biology and Environmental Protection , Toruń , Poland and.
authors.forename: Michał
authors.initials: M
authors.lastname: Caputa
authors.affiliation: a N. Copernicus University , Department of Animal Physiology, Faculty of Biology and Environmental Protection , Toruń , Poland and.
authors.forename: Justyna
authors.initials: J
authors.lastname: Rogalska
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001831:Body Temperature / Q000502:physiology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006979:Hyperthermia, Induced; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000378:metabolism*
chemical_list: D014810:Vitamin E; D008315:Malondialdehyde; D005978:Glutathione; D003676:Deferoxamine
keywords: Deferoxamine; glutathione; hyperthermia; neonatal anoxia; vitamin E