title: Knockdown of long non-coding RNA TP73-AS1 inhibits cell proliferation and induces apoptosis in esophageal squamous cell carcinoma.
abstract: Recent studies have shown that long non-coding RNAs (lncRNAs) are involved in a variety of biological processes and diseases in humans, including cancer. Our study serves as the first comprehensive analysis of lncRNA TP73-AS1 in esophageal cancer. We utilized a lncRNA microarray to analyze the expression profile of lncRNAs in esophageal squamous cell carcinoma. Our results show that lncRNA TP73-AS1 and BDH2 levels are generally upregulated in esophageal cancer tissues and are strongly correlated with tumor location or TNM stage in clinical samples. LncRNA TP73-AS1 knockdown inhibited BDH2 expression in EC9706 and KYSE30 cells, whereas BDH2 knockdown repressed esophageal cancer cell proliferation and induced apoptosis via the caspase-3 dependent apoptotic pathway. Overexpression of BDH2 in lncRNA TP73-AS1 knockdown cells partially rescued cell proliferation rates and suppressed apoptosis. In mouse xenografts, tumor size was reduced in lncRNA TP73-ASI siRNA-transfected tumors, suggesting that downregulation of lncRNA TP73-AS1 attenuated EC proliferation in vitro and in vivo. In addition, BDH2 or lncRNA TP73-AS1 knockdown enhanced the chemosensitivity of esophageal cancer cells to 5-FU and cisplatin. Our results suggest that lncRNA TP73-AS1 may be a novel prognostic biomarker that could serve as a potential therapeutic target for the treatment of esophageal cancer.
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.affiliation: Department of Hemato-tumor, The First Affiliated Hospital of Henan University of TCM, Zhengzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.|Collaborative Innovation Center of Cancer Chemoprevention, Henan, China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.|Collaborative Innovation Center of Cancer Chemoprevention, Henan, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D016022:Case-Control Studies; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C000607535:long non-coding RNA KIAA0495, human; C510607:BDH2 protein, human; D006884:Hydroxybutyrate Dehydrogenase
keywords: BDH2; LncRNA TP73-AS1; biomarker; chemosensitivity; esophageal cancer