title: "Deciphering Archaeal Communities" Omics Tools in the Study of Archaeal Communities.
abstract: Archaea constitute one of the three recognized phylogenetic groups of organisms living on the planet, and the latest to be discovered. Most Archaea resist cultivation and are studied using molecular methods. High-throughput amplicon sequencing and metagenomic approaches have been key in uncovering hitherto unknown archaeal diversity, their metabolic potential, and have even provided an insight into genomes of a number of uncultivated members of this group. Here, we summarize protocols describing sampling, molecular, metagenomic, and metatranscriptomic analyses as well as bioinformatics approaches that have proved useful for the study of archaea in natural samples.
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia. lejla.pasic@bf.uni-lj.si.
authors.forename: Lejla
authors.initials: L
authors.lastname: Pašić
authors.affiliation: Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, San Juan de Alicante, 03550, Alicante, Spain. amartin@umh.es.
authors.forename: Ana-Belen
authors.initials: AB
authors.lastname: Martin-Cuadrado
authors.affiliation: Unité d'Ecologie, Systématique et Evolution, CNRS UMR 8079, Université Paris-Sud, 91405, Orsay, France. puri.lopez@u-psud.fr.
authors.forename: Purificación
authors.initials: P
authors.lastname: López-García
mesh_terms: D001105:Archaea / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D056186:Metagenomics; D010802:Phylogeny
keywords: Archaeal communities; Bioinformatic analysis; Large insert-size library; Metagenomic DNA; Single-cell genomics; Small insert-size library