title: Establishment of Novel Monoclonal Antibody PMab-32 Against Rabbit Podoplanin.
abstract: Podoplanin (PDPN) is a type I transmembrane O-glycoprotein, which is known as a specific lymphatic marker. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. PDPN is also expressed in several normal tissues, including podocytes and type I alveolar cells. Although many monoclonal antibodies (MAbs) against human PDPN (hPDPN), mouse PDPN (mPDPN), and rat PDPN (rPDPN) have been established, useful antibodies against rabbit PDPN (rabPDPN) have not been developed. In this study, we immunized mice with the recombinant proteins of rabPDPN, and developed a novel anti-rabPDPN MAb, named PMab-32. PMab-32 could detect endogenous and exogenous rabPDPN in flow cytometry and Western blot analysis. The KD of PMab-32 was determined to be 6.2 × 10(-8) M by flow cytometry. Immunohistochemical analysis showed that PMab-32 is useful for detecting podocytes, type I alveolar cells, and lymphatic endothelial cells in normal rabbit tissues. PMab-32 is expected to be useful for various rabbit experiments.
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .|2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Honma
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogasawara
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .|2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Oki
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .|2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .
authors.forename: Takuro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .
authors.forename: Mika K
authors.initials: MK
authors.lastname: Kaneko
authors.affiliation: 2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
authors.forename: Michiaki
authors.initials: M
authors.lastname: Takagi
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Aoba-ku, Sendai, Japan .
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D016466:CHO Cells; D003412:Cricetulus; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D050199:Podocytes / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins