title: Multiple myeloma cell lines and primary tumors proteoma: protein biosynthesis and immune system as potential therapeutic targets.
abstract: Despite great advance in multiple myeloma (MM) treatment since 2000s, it is still an incurable disease and novel therapies are welcome. Therefore, the purpose of this study was to explore MM plasma cells' (MM-PC) proteome, in comparison with their normal counterparts (derived from palatine tonsils of normal donors, ND-PC), in order to find potential therapeutic targets expressed on the surface of these cells. We also aimed to evaluate the proteome of MM cell lines with different genetic alterations, to confirm findings obtained with primary tumor cells. Bone marrow (BM) samples from eight new cases of MM and palatine tonsils from seven unmatched controls were submitted to PC separation and, in addition to two MM cell lines (U266, RPMI-8226), were submitted to protein extraction for mass spectrometry analyses. A total of 81 proteins were differentially expressed between MM-PC and ND-PC - 72 upregulated and nine downregulated; U266 vs. RPMI 8226 cell lines presented 61 differentially expressed proteins - 51 upregulated and 10 downregulated. On primary tumors, bioinformatics analyses highlighted upregulation of protein biosynthesis machinery, as well as downregulation of immune response components, such as MHC class I and II, and complement receptors. We also provided comprehensive information about U266 and RPMI-8226 cell lines' proteome and could confirm some patients' findings.
authors.affiliation: Departamento de Oncologia Clínica e Experimental, Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Rodrigo Carlini
authors.initials: RC
authors.lastname: Fernando
authors.affiliation: Departamento de Oncologia Clínica e Experimental, Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Fabricio
authors.initials: F
authors.lastname: de Carvalho
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Diego Robles
authors.initials: DR
authors.lastname: Mazzotti
authors.affiliation: Fundação Pio XII - Hospital de Câncer de Barretos, Barretos, Brazil.
authors.forename: Adriane Feijó
authors.initials: AF
authors.lastname: Evangelista
authors.affiliation: Departamento de Oncologia Clínica e Experimental, Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Walter Moisés Tobias
authors.initials: WMT
authors.lastname: Braga
authors.affiliation: Departamento de Oncologia Clínica e Experimental, Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Maria
authors.initials: M
authors.lastname: de Lourdes Chauffaille
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, Conselho Nacional de Pesquisa em Energia e Materiais, CNPEM, Campinas, Brazil.
authors.forename: Adriana Franco Paes
authors.initials: AFP
authors.lastname: Leme
authors.affiliation: Departamento de Oncologia Clínica e Experimental, Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Gisele Wally Braga
authors.initials: GWB
authors.lastname: Colleoni
keywords: multiple myeloma; proteomics; therapeutic targets