title: [Application progress of CRISPR/Cas9 genome editing technology in the treatment of HIV-1 infection].
abstract: The goal of gene therapy is to introduce foreign genes into human target cells in a certain way to correct or compensate diseases caused by defective or abnormal genes. Therefore, gene therapy has great practical significance in studying the treatment of persistent or latent HIV-1 infection. At present, the existing methods of gene therapy have some major defects such as limited target site recognition and high frequency of off-targets. The latest research showed that the clustered regularly interspaced short palindromic repeats (CRISPR) /CRISPR-associated nuclease 9 (Cas9) system from bacteria and archaea has been successfully reformed to a targeted genome editing tool. Thus, how to achieve the goal of treating HIV-1 infection by modifying targeted HIV-1 virus genome effectively using the CRISPR/Cas9 system has become a current research focus. Here we review the latest achievements worldwide and briefly introduce applications of the CRISPR/Cas9 genome editing technology in the treatment of HIV-1 infection, including CCR5 gene editing, removal of HIV-1 virus and activation of HIV-1 virus, in order to provide reference for the prevention and treatment of HIV-1 infection.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China;Lamprey Research Center, Liaoning Normal University, Dalian 116029, China;
authors.forename: Ying-lun
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China; Lamprey Research Center, Liaoning Normal University, Dalian 116029, China;
authors.forename: Qing-wei
authors.initials: QW
authors.lastname: Li
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D015316:Genetic Therapy / Q000295:instrumentation / Q000379:methods*; D015658:HIV Infections / Q000235:genetics* / Q000628:therapy / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D017393:RNA Editing
chemical_list: D004720:Endonucleases