title: Mesenteric IL-10-producing CD5+ regulatory B cells suppress cow's milk casein-induced allergic responses in mice.
abstract: Food allergy is a hypersensitive immune reaction to food proteins. We have previously demonstrated the presence of IL-10-producing CD5(+) B cells and suggested their potential role in regulating cow's milk casein allergy in humans and IgE-mediated anaphylaxis in mice. In this study, we determined whether IL-10-producing CD5(+) regulatory B cells control casein-induced food allergic responses in mice and, if so, the underlying mechanisms. The induction of oral tolerance (OT) by casein suppressed casein-induced allergic responses including the decrease of body temperature, symptom score, diarrhea, recruitment of mast cells and eosinophils into jejunum, and other biological parameters in mice. Notably, the population of IL-10-producing CD5(+) B cells was increased in mesenteric lymph node (MLN), but not in spleen or peritoneal cavity (PeC) in OT mice. The adoptive transfer of CD5(+) B cells from MLN, but not those from spleen and PeC, suppressed the casein-induced allergic responses in an allergen-specific and IL-10-dependent manner. The inhibitory effect of IL-10-producing CD5(+) B cells on casein-induced allergic response was dependent on Foxp3(+) regulatory T cells. Taken together, mesenteric IL-10-producing regulatory B cells control food allergy via Foxp3(+) regulatory T cells and could potentially act as a therapeutic regulator for food allergy.
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: A-Ram
authors.initials: AR
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyuk Soon
authors.initials: HS
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Do Kyun
authors.initials: DK
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Seung Taek
authors.initials: ST
authors.lastname: Nam
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Dajeong
authors.initials: D
authors.lastname: Lee
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Min Bum
authors.initials: MB
authors.lastname: Lee
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD20892.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Beaven
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology*; D000818:Animals; D016175:B-Lymphocyte Subsets; D060151:B-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism*; D018956:CD5 Antigens; D002364:Caseins / Q000276:immunology*; D002417:Cattle; D002450:Cell Communication; D004195:Disease Models, Animal; D005260:Female; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000276:immunology; D056747:Immunomodulation; D016753:Interleukin-10 / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008643:Mesentery; D051379:Mice; D008892:Milk / Q000276:immunology*; D016269:Milk Hypersensitivity / Q000276:immunology* / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; D018956:CD5 Antigens; D002364:Caseins; D016753:Interleukin-10; D007073:Immunoglobulin E