title: Tissue-of-origin-specific gene repositioning in breast and prostate cancer.
abstract: Genes have preferential non-random spatial positions within the cell nucleus. The nuclear position of a subset of genes differ between cell types and some genes undergo repositioning events in disease, including cancer. It is currently unclear whether the propensity of a gene to reposition reflects an intrinsic property of the locus or the tissue. Using quantitative FISH analysis of a set of genes which reposition in cancer, we test here the tissue specificity of gene repositioning in normal and malignant breast or prostate tissues. We find tissue-specific organization of the genome in normal breast and prostate with 40 % of genes occupying differential positions between the two tissue types. While we demonstrate limited overlap between gene sets that repositioned in breast and prostate cancer, we identify two genes that undergo disease-related gene repositioning in both cancer types. Our findings indicate that gene repositioning in cancer is tissue-of-origin specific.
authors.affiliation: National Cancer Institute, NIH, Bethesda, MD, 20892, USA. meaburnk@mail.nih.gov.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Meaburn
authors.affiliation: National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
authors.forename: Olufunmilayo
authors.initials: O
authors.lastname: Agunloye
authors.affiliation: National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Devine
authors.affiliation: National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Leshner
authors.affiliation: National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Roloff
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: True
authors.affiliation: National Cancer Institute, NIH, Bethesda, MD, 20892, USA. mistelit@mail.nih.gov.
authors.forename: Tom
authors.initials: T
authors.lastname: Misteli
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009928:Organ Specificity / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*
keywords: Breast cancer; Nuclear architecture; Prostate cancer; Spatial gene positioning; Tissue specificity