title: Phenotypic characterization of nanshi oral liquid alters metabolic signatures during disease prevention.
abstract: This paper was designed to investigate the phenotypic characterization of Nanshi Oral Liquid (NOL) alters metabolic signatures of the 'Kidney Yang Deficiency syndrome' (KYDS). Urine metabolites were profiled by UPLC-ESI-Q-TOF-HDMS. The significantly changed metabolites such as xanthurenic acid, 4,8-dihydroxyquinoline, 3-methyldioxyindole, 4,6-dihydroxyquinoline, kynurenic acid, hippuric acid, taurine, tyramine, and 3-metanephrine, had been identified, and were related to the disturbance in tyrosine metabolism, steroid hormone biosynthesis, taurine and hypotaurine metabolism, tryptophan metabolism, phenylalanine metabolism and lysine degradation, which were helpful to further understanding the KYDS and intervention mechanism of NOL. The biochemical result showed that NOL can alleviate the kidney impairment induced by KYDS. Metabolomics results indicated the significantly changed metabolites were found to be reasonable in explaining the action mechanism of NOL. Interestingly, the effectiveness of NOL against KYDS was proved using the established metabolomics method and regulated the biomarkers as well as adjusted the metabolic disorder pathways. NOL had potentially pharmacological effect through regulating multiple perturbed pathways to normal state. This work showed that the metabolomics method was a powerful approach for studying the phenotypic characterization of disease's syndrome during disease prevention and its intervention mechanism.
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|Research Center of Chinmedomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|Research Center of Chinmedomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Infinitus (China) Company Ltd, Guangdong Province, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhao
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|Research Center of Chinmedomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|Department of Pharmaceutical Analysis, School of Pharmacy, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|Research Center of Chinmedomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Nan
authors.affiliation: Infinitus (China) Company Ltd, Guangdong Province, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Zou
authors.affiliation: Infinitus (China) Company Ltd, Guangdong Province, China.
authors.forename: Chung Wah
authors.initials: CW
authors.lastname: Ma
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|Research Center of Chinmedomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D007674:Kidney Diseases / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D025341:Principal Component Analysis; D051381:Rats; D013577:Syndrome; D016711:Yang Deficiency / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal