title: Properties of the Probiotic Strain Lactobacillus plantarum 8-RA-3 Grown in a Biofilm by Solid Substrate Cultivation Method.
abstract: The biofilm formation took place in 48 h within the solid substrate cultivation of Lactobacillus plantarum 8-RA-3 strain on the wheat bran saturated with the MRS medium. The drying of the bran fermented by lactobacilli resulted in a decrease in the number of colony-forming units (CFU) from 23.0 × 10(8) to 6.9 × 10(5) CFU/g in daily samples and to less than 10(4) CFU/g in 2- and 3-day samples. However, according to the fluorescence-based live/dead assay data, more than 40 % of the non-cultured bacteria were viable. As a result of mice kept on a diet with the introduction of bran fermented by Lact. plantarum 8-RA-3 for 72 h into the fodder, a recovery of normal level of intestinal lactobacilli, inhibited by administration of antibiotic was noted. The strain genetically identical to the Lact. plantarum 8-RA-3 was isolated from the feces of these mice. The results indicate that solid substrate cultivated Lact. plantarum 8-RA-3 strain formed a biofilm. Once dried and transferred into a non-cultured state, biofilm cells retained its viability and biological activity.
authors.affiliation: A.N. Severtsov Institute of Ecology and Evolution, Leninsky prospect, 33, 119071, Moscow, Russia.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Ushakova
authors.affiliation: Institute of Immunological Engineering, Lyubuchany, Chekhov District, 142380, Moscow Region, Russia.
authors.forename: Vyacheslav M
authors.initials: VM
authors.lastname: Abramov
authors.affiliation: Institute of Immunological Engineering, Lyubuchany, Chekhov District, 142380, Moscow Region, Russia.
authors.forename: Valentin S
authors.initials: VS
authors.lastname: Khlebnikov
authors.affiliation: Moscow State University, Lenin's Hills, 1/12, 119992, Moscow, Russia.
authors.forename: Alexandr M
authors.initials: AM
authors.lastname: Semenov
authors.affiliation: "Bioengineering" Centre, Prospekt 60-Letiya Oktyabrya, 7/1, 117312, Moscow, Russia.
authors.forename: Boris B
authors.initials: BB
authors.lastname: Kuznetsov
authors.affiliation: A.N. Severtsov Institute of Ecology and Evolution, Leninsky prospect, 33, 119071, Moscow, Russia.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Kozlova
authors.affiliation: A.N. Severtsov Institute of Ecology and Evolution, Leninsky prospect, 33, 119071, Moscow, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Nifatov
authors.affiliation: Institute of Immunological Engineering, Lyubuchany, Chekhov District, 142380, Moscow Region, Russia.
authors.forename: Vadim K
authors.initials: VK
authors.lastname: Sakulin
authors.affiliation: Institute of Immunological Engineering, Lyubuchany, Chekhov District, 142380, Moscow Region, Russia.
authors.forename: Igor V
authors.initials: IV
authors.lastname: Kosarev
authors.affiliation: Institute of Immunological Engineering, Lyubuchany, Chekhov District, 142380, Moscow Region, Russia.
authors.forename: Raisa N
authors.initials: RN
authors.lastname: Vasilenko
authors.affiliation: "Bioengineering" Centre, Prospekt 60-Letiya Oktyabrya, 7/1, 117312, Moscow, Russia.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Sukhacheva
authors.affiliation: International Science and Technology Center, Krasnoproletarskaya ulitsa, 32-34, 127473, Moscow, Russia. melnikov@istc.ru.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Melnikov
keywords: Biofilm; Lactobacillus plantarum; Non-cultured cells; Probiotics; Solid substrate cultivation; Wheat bran