title: Low sulfide levels and a high degree of cystathionine β-synthase (CBS) activation by S-adenosylmethionine (SAM) in the long-lived naked mole-rat.
abstract: Hydrogen sulfide (H2S) is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS), an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration.
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany. Electronic address: maja.dziegelewska@leibniz-fli.de.
authors.forename: Maja
authors.initials: M
authors.lastname: Dziegelewska
authors.affiliation: Reproduction Management, Leibniz Institute for Zoo & Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany. Electronic address: holtze@izw-berlin.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Holtze
authors.affiliation: Department of General Zoology, University of Duisburg-Essen, Universitätsstraße 2, 45141 Essen, Germany. Electronic address: christiane.vole@uni-due.de.
authors.forename: Christiane
authors.initials: C
authors.lastname: Vole
authors.affiliation: Klinik für Anästhesiologie, Universitätsklinikum, Albert-Einstein-Allee 23, 89081 Ulm, Germany. Electronic address: ulrich.wachter@uni-ulm.de.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wachter
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology - Hans-Knöll-Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany. Electronic address: Uwe.Menzel@hki-jena.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Menzel
authors.affiliation: Reproduction Management, Leibniz Institute for Zoo & Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany. Electronic address: morhart@izw-berlin.de.
authors.forename: Michaela
authors.initials: M
authors.lastname: Morhart
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany. Electronic address: marco.groth@leibniz-fli.de.
authors.forename: Marco
authors.initials: M
authors.lastname: Groth
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany. Electronic address: karol.szafranski@leibniz-fli.de.
authors.forename: Karol
authors.initials: K
authors.lastname: Szafranski
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany. Electronic address: arne.sahm@leibniz-fli.de.
authors.forename: Arne
authors.initials: A
authors.lastname: Sahm
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany; Department of Anaesthesiology and Intensive Care Therapy, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany. Electronic address: Christoph.Sponholz@med.uni-jena.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Sponholz
authors.affiliation: Department of General Zoology, University of Duisburg-Essen, Universitätsstraße 2, 45141 Essen, Germany; Central Animal Laboratory, University Hospital, University of Duisburg-Essen, Hufelandstraße 55, 45122 Essen, Germany. Electronic address: Philip.Dammann@uk-essen.de.
authors.forename: Philip
authors.initials: P
authors.lastname: Dammann
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany. Electronic address: klaus.huse@leibniz-fli.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Huse
authors.affiliation: Reproduction Management, Leibniz Institute for Zoo & Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany. Electronic address: HILDEBRANDT@izw-berlin.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hildebrandt
authors.affiliation: Genome Analysis, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany. Electronic address: matthias.platzer@leibniz-fli.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Platzer
mesh_terms: D000375:Aging / Q000097:blood* / Q000473:pathology; D000818:Animals; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism*; D004032:Diet; D006862:Hydrogen Sulfide / Q000097:blood*; D008099:Liver / Q000201:enzymology; D008136:Longevity / Q000235:genetics; D008715:Methionine / Q000378:metabolism; D019577:Mole Rats; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism*
chemical_list: D012436:S-Adenosylmethionine; D008715:Methionine; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide
keywords: Hydrogen sulfide; Longevity; Low methionine diet; Methionine; Mole-rats; Transsulfuration