title: EGFR MUTATIONS IN NON-SMALL CELL LUNG CANCER: LOCAL EPIDEMIOLOGY AND CLINICAL IMPORTANCE.
abstract: BACKGROUND
  Lung cancer's dismal prognosis led to new therapeutic approaches among which TKIs being among most promising;
  
  
  METHODS
  Retrospective study at the Regional Institute of Oncology Iasi of non small cell lung cancer patients which underwent molecular investigations between November 2013 - September 2014. EGFR mutation status (positive, negative, undetermined) was assessed with an Entrogen EGFR kit using DNA extracted from paraffin embedded samples (surgical or endobronchial biopsies) with the Macherey-Nagel "NucleoSpin FFPE DNAkit" and then amplified on a Applied Biosystem 7500 Real Time PCR System.
  
  
  RESULTS
  There were 63 adenocarcinoma samples (17 females, mean age 60,9 +/- 9 years): 49 primary lung tumors and 14 secondary lesions (brain, lymph nodes, pleural). There was insufficient bioptic material for three cases. TTF1 status was determined for 46 patients--six were negative. There were twelve mutations identified (7 female subjects, 5 male)--six L858R, five Del 19 and one G719X; ten were TTF1 positive for the remaining two TTF1 status was unknown. Female sex predominance was statistically significant (p = 0.02, chi squared). Mean age for mutation positive patients was 64 +/- 10 years; there were three never smokers, three active smokers and no data on smoking status was available for six subjects.
  
  
  CONCLUSIONS
  Although small dimension of the study group precludes statistical significance EGFR mutations seem to correlate with TTF1 status.
authors.forename: F
authors.initials: F
authors.lastname: Rusu-Cordunean
authors.forename: Mădălina Lavinia
authors.initials: ML
authors.lastname: Berlea
authors.forename: A T
authors.initials: AT
authors.lastname: Cernomaz
authors.forename: M V
authors.initials: MV
authors.lastname: Marinca
authors.forename: Simona
authors.initials: S
authors.lastname: Peter
authors.forename: Ina
authors.initials: I
authors.lastname: Pavel
authors.forename: B D
authors.initials: BD
authors.lastname: Grigoriu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D018773:Genes, erbB-1 / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012307:Risk Factors; D012383:Romania / Q000453:epidemiology; D012680:Sensitivity and Specificity; D017678:Sex Distribution; D014157:Transcription Factors
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C504003:TTF1 protein, human; D014157:Transcription Factors