title: Proteomic analysis of the plasma membrane-movement tubule complex of cowpea mosaic virus.
abstract: Cowpea mosaic virus forms tubules constructed from the movement protein (MP) in plasmodesmata (PD) to achieve cell-to-cell movement of its virions. Similar tubules, delineated by the plasma membrane (PM), are formed protruding from the surface of infected protoplasts. These PM-tubule complexes were isolated from protoplasts by immunoprecipitation and analysed for their protein content by tandem mass spectrometry to identify host proteins with affinity for the movement tubule. Seven host proteins were abundantly present in the PM-tubule complex, including molecular chaperonins and an AAA protein. Members of both protein families have been implicated in establishment of systemic infection. The potential role of these proteins in tubule-guided cell-cell transport is discussed.
authors.affiliation: Laboratory of Virology, Plant Sciences Group, Wageningen University, Wageningen, The Netherlands.
authors.forename: Paulus W
authors.initials: PW
authors.lastname: den Hollander
authors.affiliation: Laboratory of Molecular Plant Virology, Department of Phytopathology, Federal University of Lavras, Lavras, Brazil.
authors.forename: Priscilla
authors.initials: P
authors.lastname: de Sousa Geraldino Duarte
authors.affiliation: Laboratory of Virology, Plant Sciences Group, Wageningen University, Wageningen, The Netherlands.
authors.forename: Hanke
authors.initials: H
authors.lastname: Bloksma
authors.affiliation: Laboratory of Biochemistry, Agrotechnology and Food Sciences, Wageningen University, Wageningen, The Netherlands.
authors.forename: Sjef
authors.initials: S
authors.lastname: Boeren
authors.affiliation: Laboratory of Virology, Plant Sciences Group, Wageningen University, Wageningen, The Netherlands. Jan.vanlent@wur.nl.
authors.forename: Jan W M
authors.initials: JW
authors.lastname: van Lent
mesh_terms: D015153:Blotting, Western; D002462:Cell Membrane / Q000821:virology*; D017798:Comovirus / Q000235:genetics* / Q000502:physiology; D007887:Fabaceae / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000502:physiology*; D031425:Plasmodesmata / Q000821:virology; D040901:Proteomics; D011523:Protoplasts / Q000821:virology
chemical_list: D053830:Plant Viral Movement Proteins