title: Expression of a chitin deacetylase gene, up-regulated in Cryptococcus laurentii strain RY1, under nitrogen limitation.
abstract: This study reports the identification of a chitin deacetylase gene in Cryptococcus laurentii strain RY1 over-expressing under nitrogen limitation by differential display. The up-regulation took place in robustly growing cells rather than in starving quiescent autophagic cells. Quantitative Real Time-PCR, enzyme activity in cell lysate and cell wall analysis corroborated the up-regulation of chitin deacetylase under nitrogen limitation. These results suggest chitin deacetylase might play a significant role in nitrogen limiting growth of Cryptococcus laurentii strain RY1.
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Writachit
authors.initials: W
authors.lastname: Chakraborty
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Soumyadev
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Somnath
authors.initials: S
authors.lastname: Chakravorty
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Semantee
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Debanjana
authors.initials: D
authors.lastname: Bhattacharya
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Ratan
authors.initials: R
authors.lastname: Gachhui
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics*; D003454:Cryptococcus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D000068858:Kombucha Tea / Q000382:microbiology; D009584:Nitrogen / Q000172:deficiency*; D015854:Up-Regulation
chemical_list: D000068858:Kombucha Tea; D000581:Amidohydrolases; C019866:chitin deacetylase; D009584:Nitrogen
keywords: Chitin deacetylase; Cryptococcus laurentii strain RY1; Nitrogen limitation; Over-expressing