title: Obestatin Accelerates the Healing of Acetic Acid-Induced Colitis in Rats.
abstract: Obestatin, a 23-amino acid peptide derived from the proghrelin, has been shown to exhibit some protective and therapeutic effects in the gut. The aim of present study was to determine the effect of obestatin administration on the course of acetic acid-induced colitis in rats. Materials and Methods. Studies have been performed on male Wistar rats. Colitis was induced by a rectal enema with 3.5% acetic acid solution. Obestatin was administered intraperitoneally twice a day at a dose of 8 nmol/kg, starting 24 h after the induction of colitis. Seven or 14 days after the induction of colitis, the healing rate of the colon was evaluated. Results. Treatment with obestatin after induction of colitis accelerated the healing of colonic wall damage and this effect was associated with a decrease in the colitis-evoked increase in mucosal activity of myeloperoxidase and content of interleukin-1β. Moreover, obestatin administration significantly reversed the colitis-evoked decrease in mucosal blood flow and DNA synthesis. Conclusion. Administration of exogenous obestatin exhibits therapeutic effects in the course of acetic acid-induced colitis and this effect is related, at least in part, to the obestatin-evoked anti-inflammatory effect, an improvement of local blood flow, and an increase in cell proliferation in colonic mucosa.
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 16 Grzegorzecka Street, 31-531 Cracow, Poland; Department of Anatomy, Jagiellonian University Medical College, 12 Kopernika Street, 31-034 Cracow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Matuszyk
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 16 Grzegorzecka Street, 31-531 Cracow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ceranowicz
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 16 Grzegorzecka Street, 31-531 Cracow, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Warzecha
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 16 Grzegorzecka Street, 31-531 Cracow, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Cieszkowski
authors.affiliation: Department of Medical Physiology, Faculty of Health Sciences, Jagiellonian University Medical College, 12 Michałowskiego Street, 31-126 Cracow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bonior
authors.affiliation: Department of Medical Physiology, Faculty of Health Sciences, Jagiellonian University Medical College, 12 Michałowskiego Street, 31-126 Cracow, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jaworek
authors.affiliation: Chair of Clinical Biochemistry, Department of Diagnostics, Jagiellonian University Medical College, 15a Kopernika Street, 31-501 Cracow, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Kuśnierz-Cabala
authors.affiliation: Department of Internal Medicine II, Thuringia Clinic, Teaching Hospital of the University of Jena, Rainweg 68, 07318 Saalfeld, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Konturek
authors.affiliation: Faculty of Physical Education and Sport, University of Physical Education, 78 Jan Paweł II Street, 31-571 Cracow, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Ambroży
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 16 Grzegorzecka Street, 31-531 Cracow, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Dembiński
mesh_terms: D019342:Acetic Acid; D000818:Animals; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004247:DNA / Q000096:biosynthesis; D054439:Ghrelin / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000378:metabolism; D007413:Intestinal Mucosa / Q000098:blood supply / Q000187:drug effects; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D054439:Ghrelin; D053583:Interleukin-1beta; D012965:Sodium Chloride; D004247:DNA; D009195:Peroxidase; D019342:Acetic Acid