title: Ultrastructural and Cytochemical Properties of Peripheral Blood Cells of Piebald Naked Carp (Gymnocypris eckloni).
abstract: The ultrastructural and cytochemical properties of peripheral blood cells of Gymnocypris eckloni were investigated by transmission electron microscopy and a range of cytochemical techniques to provide clear insight into the structure and function of blood cells from this fish. Ultrastructurally, erythrocytes, leucocytes (neutrophils, eosinophils, lymphocytes, monocytes), thrombocytes and plasma cells were identified in the peripheral blood of G. eckloni. The most special ultrastructural characteristics of blood cells in this fish were that neutrophils exhibited only one type of cytoplasmic granules containing an eccentric, spherical or oval electron-dense core, and eosinophils presented two types of granules with non-uniform electronic density and without crystalloids in their cytoplasm. Neutrophils, eosinophils, lymphocytes, monocytes and thrombocytes were positive for periodic acid-Schiff and α-naphthyl acetate esterase staining. Intense peroxidase positive staining was observed in neutrophils and monocytes, but not in eosinophils, lymphocytes and thrombocytes. Neutrophils, eosinophils and monocytes were stained positively for acid phosphatase, whereas lymphocytes and thrombocytes did not stain. Leucocytes and thrombocytes were negative for alkaline phosphatase and Sudan black B staining. Erythrocytes were negative for all cytochemical staining. The cytochemical and ultrastructural features of peripheral blood cells of G. eckloni were similar to those of other fish species. However, some important differences were identified in G. eckloni.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Zheng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: J
authors.initials: J
authors.lastname: Fang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: X
authors.initials: X
authors.lastname: Peng
authors.affiliation: Colleage of Biological and Agro-forestry Engineering, Tongren University, Tongren, Guizhou, 554300, China.
authors.forename: J D
authors.initials: JD
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Yang
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000648:ultrastructure*; D002347:Carps / Q000097:blood*; D004912:Erythrocytes / Q000166:cytology / Q000648:ultrastructure*; D006651:Histocytochemistry / Q000662:veterinary; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary*; D009000:Monocytes / Q000166:cytology / Q000648:ultrastructure*; D009504:Neutrophils / Q000166:cytology / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000662:veterinary
chemical_list: D000135:Acid Phosphatase