title: A new ratiometric fluorescent probe for rapid, sensitive and selective detection of endogenous hydrogen sulfide in mitochondria.
abstract: We have developed a new ratiometric fluorescent probe composed of a coumarin-merocyanine dyad based on the FRET mechanism. The probe showed clear dual-emission signal changes in blue and red spectral windows upon addition of H2S in a dose dependent manner under a single wavelength excitation. The probe targeted mitochondria with high selectivity and sensitivity toward H2S.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China. bxzhao@sdu.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P. R. China. miaojy@sdu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China. bxzhao@sdu.edu.cn.
authors.forename: Jin-Ting
authors.initials: JT
authors.lastname: Liu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P. R. China. miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China. bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006862:Hydrogen Sulfide / Q000032:analysis*; D057230:Limit of Detection; D008928:Mitochondria / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide