title: Nocardiopsis mwathae sp. nov., isolated from the haloalkaline Lake Elmenteita in the African Rift Valley.
abstract: During a screening for novel and biotechnologically useful bacteria in haloalkaline lakes, strain No.156(T) was isolated from a sediment sample from lake Elmenteita in the African Rift Valley and studied by a polyphasic taxonomic approach. The strain was observed to form yellow aerial and substrate mycelia; optimal growth was found to be at 30-35 °C in salt concentrations of 6-9 % (w/v) and at pH 7-9. The DNA G+C content of the novel strain was 71 mol%. Analysis of 16S rRNA sequences indicated that the isolate belongs to the genus Nocardiopsis with sequence similarities below 98 % to the type strains of all other representatives of the genus. Mycolic acids were not detected in whole cell methanolysates. The peptidoglycan was found to contain meso-diaminopimelic acid as the diamino acid with no diagnostic sugars. The main polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol but no diphosphatidylglycerol. The predominant menaquinones were MK-11(H8), MK-11(H6), MK-10(H8) and MK-10(H6). Cellular fatty acids were found to consist of saturated and monounsaturated iso- and anteiso-branched acids with 16-18 C-length, tuberculostearic acid (Me18:0), and straight-chain saturated (16:0, 18:0) acids. These characteristics match those of the genus Nocardiopsis. Based on 16S rRNA gene sequence analysis and phenotypic characteristics, a novel species with the name Nocardiopsis mwathae is proposed. The type strain is No.156(T) (=DSM 46659(T) = CECT 8552(T)). The INSDC accession number for the 16S rRNA gene sequence of strain No.156(T) is KF976731.
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.|Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
authors.forename: Juliah Khayeli
authors.initials: JK
authors.lastname: Akhwale
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Göker
authors.affiliation: Central Facility for Microscopy, HZI-Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Taita Taveta University College, P.O Box 635-80300, Voi, Kenya. hamadiboga@ttuc.ac.ke.
authors.forename: Hamadi Iddi
authors.initials: HI
authors.lastname: Boga
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany. hans-peter.klenk@ncl.ac.uk.|School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK. hans-peter.klenk@ncl.ac.uk.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Haloalkaline; Polyphasic taxonomy; Soda lake; Streptosporangineae