title: Increased matrix metalloproteinase-2 expression and reduced tissue factor pathway inhibitor-2 expression correlate with angiogenesis and early postoperative recurrence of pancreatic carcinoma.
abstract: Matrix metalloproteinase (MMP)-2 and tissue factor pathway inhibitor (TFPI)-2 are known to influence tumor angiogenesis and progression. This work aimed to describe the levels of MMP-2 and TFPI-2 expression associated with tumor angiogenesis and early postoperative recurrence in patients with pancreatic carcinoma. Expression of MMP-2 and TFPI-2 in carcinoma tissues and paracarcinomatous tissues was assayed by immunostaining. Expression of vascular endothelial growth factor (VEGF) and CD34 in tumor tissues was also assayed by immunostaining. The correlations of MMP-2 and TFPI-2 with VEGF, microvessel density (MVD), and early postoperative recurrence were analyzed. The results showed that MMP-2 expression was significantly increased (P < 0.05) and TFPI-2 expression was significantly decreased (P < 0.001) in carcinoma tissues compared with paracarcinomatous tissues. MMP-2 expression was positively correlated with VEGF (r = 0.594, P < 0.001) and MVD (r = 0.432, P < 0.001) in carcinoma tissues. TFPI-2 expression was negatively correlated with VEGF (r = -0.654, P < 0.001) and MVD (r = -0.360, P < 0.001) in carcinoma tissues. Multivariate logistic regression analysis showed that up-regulated MMP-2 and down-regulated TFPI-2 were independent predictors of early postoperative recurrence of pancreatic carcinoma. Receiver operating characteristic curve analysis showed that the combination of MMP-2 and TFPI-2 was a reliable predictive model of early recurrence. We conclude that increased MMP-2 expression and reduced TFPI-2 expression are closely linked to angiogenesis and early postoperative recurrence of pancreatic carcinoma. Immunohistochemical assay of MMP-2 and TFPI-2 may be useful for predicting early relapse of pancreatic carcinoma after surgery.
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical UniversityHefei 230001, People's Republic of China; Anhui Province Key Laboratory of Hepatopancreatobiliary SurgeryHefei 230001, People's Republic of China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Zhai
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical UniversityHefei 230001, People's Republic of China; Anhui Province Key Laboratory of Hepatopancreatobiliary SurgeryHefei 230001, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical UniversityHefei 230001, People's Republic of China; Anhui Province Key Laboratory of Hepatopancreatobiliary SurgeryHefei 230001, People's Republic of China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical UniversityHefei 230001, People's Republic of China; Anhui Province Key Laboratory of Hepatopancreatobiliary SurgeryHefei 230001, People's Republic of China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Tang
keywords: MMP-2; TFPI-2; angiogenesis; pancreatic carcinoma; recurrence