title: Application of alkyl polyglycoside surfactant in ultrasonic-assisted extraction followed by macroporous resin enrichment for the separation of vitexin-2″-O-rhamnoside and vitexin from Crataegus pinnatifida leaves.
abstract: An alkyl polyglycoside (APG) surfactant was used in ultrasonic-assisted extraction to effectively extract vitexin-2″-O-rhamnoside (VOR) and vitexin (VIT) from Crataegus pinnatifida leaves. APG0810 was selected as the surfactant. The extraction process was optimized for ultrasonic power, the APG concentration, ultrasonic time, soaking time, and liquid-solid ratio. The proposed approach showed good recovery (99.80-102.50% for VOR and 98.83-103.19% for VIT) and reproducibility (relative standard deviation, n=5; 3.7% for VOR and 4.2% for VIT) for both components. The proposed sample preparation method is both simple and effective. The use of APG for extraction of key herbal ingredients shows great potential. Ten widely used commercial macroporous resins were evaluated in a screening study to identify a suitable resin for the separation and purification of VOR and VIT. After comparing static and dynamic adsorption and desorption processes, HPD100B was selected as the most suitable resin. After column adsorption and desorption on this resin, the target compounds VOR and VIT can be effectively separated from the APG0810 extraction solution. Recoveries of VOR and VIT were 89.27%±0.42% and 85.29%±0.36%, respectively. The purity of VOR increased from 35.0% to 58.3% and the purity of VIT increased from 12.5% to 19.9%.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.affiliation: College of Animal Science and Technology, Hebei North University, 075000, Zhangjiakou, China.
authors.forename: Yupin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China. Electronic address: ghuiyan@nefu.edu.cn.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Gu
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Fenglan
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Hu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. Electronic address: yanglei@nefu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D047310:Apigenin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D004355:Drug Stability; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D013010:Sonication / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013501:Surface-Active Agents; C021329:vitexin rhamnoside; D047310:Apigenin; C032731:vitexin
keywords: Alkyl polyglycoside; Crataegus pinnatifida; Macroporous resin; Ultrasonic-assisted extraction; Vitexin; Vitexin-2″-O-rhamnoside