title: Intra- and Interspecies Effects of Outer Membrane Vesicles from Stenotrophomonas maltophilia on β-Lactam Resistance.
abstract: The treatment ofStenotrophomonas maltophiliainfection with β-lactam antibiotics leads to increased release of outer membrane vesicles (OMVs), which are packed with two chromosomally encoded β-lactamases. Here, we show that these β-lactamase-packed OMVs are capable of establishing extracellular β-lactam degradation. We also show that they dramatically increase the apparent MICs of imipenem and ticarcillin for the cohabituating speciesPseudomonas aeruginosaandBurkholderia cenocepacia.
authors.affiliation: Laboratory for Protein Biochemistry and Biomolecular Engineering (L-PROBE), Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium.
authors.forename: Simon
authors.initials: S
authors.lastname: Devos
authors.affiliation: Laboratory for General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Ghent, Belgium.
authors.forename: Stephan
authors.initials: S
authors.lastname: Stremersch
authors.affiliation: Laboratory for General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Ghent, Belgium.
authors.forename: Koen
authors.initials: K
authors.lastname: Raemdonck
authors.affiliation: Laboratory for General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Ghent, Belgium.
authors.forename: Kevin
authors.initials: K
authors.lastname: Braeckmans
authors.affiliation: Laboratory for Protein Biochemistry and Biomolecular Engineering (L-PROBE), Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium bart.devreese@ugent.be.
authors.forename: Bart
authors.initials: B
authors.lastname: Devreese
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D057508:Burkholderia cenocepacia / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D002462:Cell Membrane / Q000737:chemistry; D003227:Conjugation, Genetic; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000201:enzymology*; D015870:Gene Expression; D022761:Gene Transfer, Horizontal; D006868:Hydrolysis; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D013982:Ticarcillin / Q000494:pharmacology; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D001618:beta-Lactamases; D013982:Ticarcillin