title: Temporal changes of bacterial communities in the Tuber melanosporum ectomycorrhizosphere during ascocarp development.
abstract: Ectomycorrhizae create a multitrophic ecosystem formed by the association between tree roots, mycelium of the ectomycorrhizal fungus, and a complex microbiome. Despite their importance in the host tree's physiology and in the functioning of the ectomycorrhizal symbiosis, detailed studies on ectomycorrhiza-associated bacterial community composition and their temporal dynamics are rare. Our objective was to investigate the composition and dynamics of Tuber melanosporum ectomycorrhiza-associated bacterial communities from summer to winter seasons in a Corylus avellana tree plantation. We used 16S ribosomal RNA (rRNA)-based pyrosequencing to compare the bacterial community structure and the richness in T. melanosporum's ectomycorrhizae with those of the bulk soil. The T. melanosporum ectomycorrhizae harbored distinct bacterial communities from those of the bulk soil, with an enrichment in Alpha- and Gamma-proteobacteria. In contrast to the bacterial communities of truffle ascocarps that vastly varies in composition and richness during the maturation of the fruiting body and to those from the bulk soil, T. melanosporum ectomycorrhiza-associated bacterial community composition stayed rather stable from September to January. Our results fit with a recent finding from the same experimental site at the same period that a continuous supply of carbohydrates and nitrogen occurs from ectomycorrhizae to the fruiting bodies during the maturation of the ascocarps. We propose that this creates a stable niche in the ectomycorrhizosphere although the phenology of the tree changes.
authors.affiliation: UMR1136 INRA Université de Lorraine, Interactions Arbres - Microorganismes, F-54280, Champenoux, France. deveau@nancy.inra.fr.|Université de Lorraine, UMR1136, Interactions Arbres - Microorganismes, F-54500, Vandœuvre-lès-Nancy, France. deveau@nancy.inra.fr.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Deveau
authors.affiliation: UMR1136 INRA Université de Lorraine, Interactions Arbres - Microorganismes, F-54280, Champenoux, France.|Université de Lorraine, UMR1136, Interactions Arbres - Microorganismes, F-54500, Vandœuvre-lès-Nancy, France.|Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Antony-Babu
authors.affiliation: UMR1136 INRA Université de Lorraine, Interactions Arbres - Microorganismes, F-54280, Champenoux, France.|Université de Lorraine, UMR1136, Interactions Arbres - Microorganismes, F-54500, Vandœuvre-lès-Nancy, France.
authors.forename: François
authors.initials: F
authors.lastname: Le Tacon
authors.affiliation: Université de Lorraine, UMR 1121, Agronomie & Environnement, Nancy-Colmar, 54500, Vandœuvre-lès-Nancy, France.|INRA, UMR 1121, Agronomie & Environnement, Nancy-Colmar, Centre INRA de Nancy-Lorraine, 54500, Vandœuvre-lès-Nancy, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Robin
authors.affiliation: UMR1136 INRA Université de Lorraine, Interactions Arbres - Microorganismes, F-54280, Champenoux, France.|Université de Lorraine, UMR1136, Interactions Arbres - Microorganismes, F-54500, Vandœuvre-lès-Nancy, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Frey-Klett
authors.affiliation: UMR1136 INRA Université de Lorraine, Interactions Arbres - Microorganismes, F-54280, Champenoux, France.|Université de Lorraine, UMR1136, Interactions Arbres - Microorganismes, F-54500, Vandœuvre-lès-Nancy, France.|INRA, UR1138, Biogéochimie des écosystèmes forestiers, F-54280, Champenoux, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Uroz
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000502:physiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D031211:Corylus / Q000382:microbiology / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015966:Gene Expression Regulation, Fungal; D038821:Mycorrhizae / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology; D012988:Soil Microbiology; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal
keywords: 16S rRNA-based pyrosequencing; Bacterial communities; Ectomycorrhizosphere; Soil-ectomycorrhizae interface; Temporal changes; Tuber melanosporum