title: S100A4 and P53 in myocardial collagen fibers of hypertrophic cardiomyopathy : Expression and clinical significance.
abstract: OBJECTIVE
  The purpose of this work is to investigate the expression of S100A4 and P53 and their correlation with myocardial collagen fibers in hypertrophic cardiomyopathy (HCM).
  
  
  METHODS
  Myocardial tissue obtained from 10 patients undergoing HCM surgery (HCM group) was compared to myocardial tissue obtained from 10 traffic accident deaths (healthy control group). Collagen volume fractions were visualized and compared using sirius red F3B(SR) staining. Immunohistochemistry S-P and in situ hybridization techniques were used to test the different expressions of S100A4, P53 proteins, and their mRNA. Imaging and statistical methods were used for quantitative analysis.
  
  
  RESULTS
  The collagen volume fraction in the HCM group was significantly higher than that in the control group. The integral optical density values of S100A4 and P53 in cardiomyocytes of the HCM group were also significantly higher than in the control group.
  
  
  CONCLUSIONS
  The increased content of collagen fibers and overexpression of S100A4 and P53 may play an important role in myocardial fibrosis of HCM, and they can be used in future HCM research. Blockade of S100A4 may have therapeutic potential in HCM by attenuating cardiac fibrosis.
authors.affiliation: Department of Medicine, Qingdao University, No. 16, Ningde Road, Shinan District, QingDao, SanDong Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Qi
authors.affiliation: Public Security Bureau, No. 128, Fuzhou Road, Shibei District, QingDao, SanDong Qingdao, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Pathology, Affiliated Hospital of Qiingdao University, No. 59, Haier Road, Laoshan District, QingDao, Shandong, China. pengzhao2015@sina.com.
authors.forename: P
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Affiliated Hospital of Qiingdao University, No. 59, Haier Road, Laoshan District, QingDao, Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Medicine, Qingdao University, No. 16, Ningde Road, Shinan District, QingDao, SanDong Shandong, China.
authors.forename: S
authors.initials: S
authors.lastname: Gong
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002312:Cardiomyopathy, Hypertrophic / Q000378:metabolism*; D005260:Female; D024022:Fibrillar Collagens / Q000378:metabolism*; D005355:Fibrosis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D015203:Reproducibility of Results; D000071999:S100 Calcium-Binding Protein A4 / Q000378:metabolism*; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D024022:Fibrillar Collagens; D000071999:S100 Calcium-Binding Protein A4; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C077591:S100A4 protein, human
keywords: Arrhythmia, cardiac; Biomarkers; Cardiac hypertrophy; Fibrosis; Sudden cardiac death