title: Evolution of DNA aptamers for malignant brain tumor gliosarcoma cell recognition and clinical tissue imaging.
abstract: Gliosarcoma, a variant of glioblastoma multiforme (GBM), is a highly invasive malignant tumor. Unfortunately, this disease still marked by poor prognosis regardless of modern treatments. It is of great significance to discover specific molecular probes targeting gliosarcoma for early cancer diagnosis and therapy. Herein, we have selected a group of DNA aptamers with high affinity and selectivity against gliosarcoma cells K308 using cell-SELEX. All the dissociation constants of these aptamers against gliosarcoma cells were in the nanomolar range and aptamer WQY-9 has the highest affinity and good selectivity among them. Furthermore, truncated aptamer sequence, WQY-9-B, shows similar recognition ability to aptamer WQY-9. In addition, WQY-9-B was found to be able to bind selectively and internalize into cytoplasm of target cancer cell at 37 °C. More importantly, compared to a random sequence, aptamer WQY-9-B showed excellent recognition rate (73.3%) for tissue sections of clinical gliosarcoma samples. These data suggests that aptamer WQY-9-B has excellent potential as an effective molecular probe for gliosarcoma diagnosis.
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Department of Neurosurgery, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
authors.forename: Qiaoyi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Department of Neurosurgery, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Department of Neurosurgery, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
authors.forename: Yuzhe
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, PR China; Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Department of Neurosurgery, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China; The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Song
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, PR China; Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Tan
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Department of Neurosurgery, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
authors.forename: Xing-Fu
authors.initials: XF
authors.lastname: Wang
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, PR China; Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jiuxing
authors.initials: J
authors.lastname: Li
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Department of Neurosurgery, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China. Electronic address: kdz99988@vip.sina.com.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Kang
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, PR China; Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China. Electronic address: cyyang@xmu.edu.cn.
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D055088:Early Detection of Cancer; D005909:Glioblastoma / Q000000981:diagnostic imaging* / Q000473:pathology; D018316:Gliosarcoma / Q000000981:diagnostic imaging* / Q000473:pathology; D006801:Humans; D015335:Molecular Probes; D052156:SELEX Aptamer Technique / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D015335:Molecular Probes
keywords: Aptamer; Biomarker; Cell-SELEX; Gliosarcoma