title: Changes in one-carbon metabolism after duodenal-jejunal bypass surgery.
abstract: Bariatric surgery alleviates obesity and ameliorates glucose tolerance. Using metabolomic and proteomic profiles, we evaluated metabolic changes in serum and liver tissue after duodenal-jejunal bypass (DJB) surgery in rats fed a normal chow diet. We found that the levels of vitamin B12in the sera of DJB rates were decreased. In the liver of DJB rats, betaine-homocysteine S-methyltransferase levels were decreased, whereas serine, cystathionine, cysteine, glutathione, cystathionine β-synthase, glutathione S-transferase, and aldehyde dehydrogenase levels were increased. These results suggested that DJB surgery enhanced trans-sulfuration and its consecutive reactions such as detoxification and the scavenging activities of reactive oxygen species. In addition, DJB rats showed higher levels of purine metabolites such as ATP, ADP, AMP, and inosine monophosphate. Decreased guanine deaminase, as well as lower levels of hypoxanthine, indicated that DJB surgery limited the purine degradation process. In particular, the AMP/ATP ratio and phosphorylation of AMP-activated protein kinase increased after DJB surgery, which led to enhanced energy production and increased catabolic pathway activity, such as fatty acid oxidation and glucose transport. This study shows that bariatric surgery altered trans-sulfuration and purine metabolism in the liver. Characterization of these mechanisms increases our understanding of the benefits of bariatric surgery.
authors.affiliation: Integrated Metabolomics Research Group, Seoul Western Center, Korea Basic Science Institute, Seoul, Republic of Korea.|KM Fundamental Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Jeeyoun
authors.initials: J
authors.lastname: Jung
authors.affiliation: Department of Surgery, College of Medicine, Hanyang University, Seoul, Republic of Korea.
authors.forename: Tae Kyung
authors.initials: TK
authors.lastname: Ha
authors.affiliation: Integrated Metabolomics Research Group, Seoul Western Center, Korea Basic Science Institute, Seoul, Republic of Korea.|Department of Chemistry, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Jueun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, School of Medicine, Keimyung University, Daegu, Republic of Korea.
authors.forename: Yunmee
authors.initials: Y
authors.lastname: Lho
authors.affiliation: Integrated Metabolomics Research Group, Seoul Western Center, Korea Basic Science Institute, Seoul, Republic of Korea.|Department of Chemistry, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Miso
authors.initials: M
authors.lastname: Nam
authors.affiliation: Integrated Metabolomics Research Group, Seoul Western Center, Korea Basic Science Institute, Seoul, Republic of Korea.
authors.forename: Doohae
authors.initials: D
authors.lastname: Lee
authors.affiliation: Diabetes Complications Research Center, UCD Conway Institute, School of Medicine and Medical Science, University College Dublin, Ireland; and.
authors.forename: Carel W
authors.initials: CW
authors.lastname: le Roux
authors.affiliation: Department of Chemistry, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Ryu
authors.affiliation: Department of Biochemistry, School of Medicine, Keimyung University, Daegu, Republic of Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Ha
authors.affiliation: Integrated Metabolomics Research Group, Seoul Western Center, Korea Basic Science Institute, Seoul, Republic of Korea gshwang@kbsi.re.kr.|Department of Life Science, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000714:Anastomosis, Surgical; D000818:Animals; D050110:Bariatric Surgery; D050940:Betaine-Homocysteine S-Methyltransferase / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003540:Cystathionine / Q000378:metabolism; D003541:Cystathionine beta-Synthase / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004386:Duodenum / Q000601:surgery*; D005227:Fatty Acids / Q000378:metabolism; D015390:Gastric Bypass; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006148:Guanine Deaminase / Q000378:metabolism; D019271:Hypoxanthine / Q000378:metabolism; D007291:Inosine Monophosphate / Q000378:metabolism; D007583:Jejunum / Q000601:surgery*; D008099:Liver / Q000378:metabolism*; D008297:Male; D055432:Metabolomics; D009765:Obesity / Q000378:metabolism / Q000601:surgery; D010084:Oxidation-Reduction; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D012694:Serine / Q000378:metabolism; D014805:Vitamin B 12 / Q000097:blood
chemical_list: D001786:Blood Glucose; D005227:Fatty Acids; D017382:Reactive Oxygen Species; D007291:Inosine Monophosphate; D019271:Hypoxanthine; D003540:Cystathionine; D000249:Adenosine Monophosphate; D012694:Serine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000444:Aldehyde Dehydrogenase; D050940:Betaine-Homocysteine S-Methyltransferase; D005982:Glutathione Transferase; D055372:AMP-Activated Protein Kinases; D006148:Guanine Deaminase; D003541:Cystathionine beta-Synthase; D005978:Glutathione; D005947:Glucose; D003545:Cysteine; D014805:Vitamin B 12
keywords: gastric bypass surgery; metabolism; metabolomics; proteomics