title: Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype.
abstract: Human bone marrow-derived stromal cells (hBMSCs) derived from the adult organism hold great promise for diverse settings in regenerative medicine. Therefore a more complete understanding of hBMSC biology to fully exploit the cells' potential for clinical settings is important. The protein CD24 has been reported to be involved in a diverse range of processes such as cancer, adaptive immunity, inflammation, and autoimmune diseases in other cell types. Its expression in hBMSCs, which has not yet been analyzed, may add an important aspect in the understanding of hBMSC biology. The present study therefore analyzes the expression, regulation, and functional implication of the surface protein CD24 in hBMSCs. Methods used are stimulation studies with TGF beta as well as shRNA-mediated knockdown and overexpression of CD24 followed by microarray, immunocytochemistry, and flow cytometric analyses. To our knowledge, we demonstrate for the first time that the expression of CD24 is an inherent property of hBMSCs. Importantly, the data links the upregulation of CD24 to the adoption of a myofibroblast-like gene expression pattern in hBMSCs. We demonstrate that CD24 is an important modulator in transforming growth factor beta 3 (TGFβ3) signaling with a reciprocal regulatory relationship between these two proteins.
authors.affiliation: Trauma Department, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Luisa Marilena
authors.initials: LM
authors.lastname: Schäck
authors.affiliation: Institute of Laboratory Animal Science, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Buettner
authors.affiliation: Cellular Neurophysiology, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wirth
authors.affiliation: Trauma Department, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Claudia
authors.identifier: 0000-0002-8537-2238
authors.initials: C
authors.lastname: Neunaber
authors.affiliation: Trauma Department, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Krettek
authors.affiliation: Trauma Department, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Hoffmann
authors.affiliation: Trauma Department, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Noack