title: MOBP levels are regulated by Fyn kinase and affect the morphological differentiation of oligodendrocytes.
abstract: Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS). Myelin is formed by extensive wrapping of oligodendroglial processes around axonal segments, which ultimately allows a rapid saltatory conduction of action potentials within the CNS and sustains neuronal health. The non-receptor tyrosine kinase Fyn is an important signaling molecule in oligodendrocytes. It controls the morphological differentiation of oligodendrocytes and is an integrator of axon-glial signaling cascades leading to localized synthesis of myelin basic protein (MBP), which is essential for myelin formation. The abundant myelin-associated oligodendrocytic basic protein (MOBP) resembles MBP in several aspects and has also been reported to be localized as mRNA and translated in the peripheral myelin compartment. The signals initiating local MOBP synthesis are so far unknown and the cellular function of MOBP remains elusive. Here, we show, by several approaches in cultured primary oligodendrocytes, that MOBP synthesis is stimulated by Fyn activity. Moreover, we reveal a new function for MOBP in oligodendroglial morphological differentiation.
authors.affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, Mainz 55128, Germany.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schäfer
authors.affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, Mainz 55128, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Müller
authors.affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, Mainz 55128, Germany.
authors.forename: Heiko J
authors.initials: HJ
authors.lastname: Luhmann
authors.affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, Mainz 55128, Germany white@uni-mainz.de.
authors.forename: Robin
authors.initials: R
authors.lastname: White
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D048430:Cell Shape; D002478:Cells, Cultured; D015870:Gene Expression; D008810:Mice, Inbred C57BL; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D009836:Oligodendroglia / Q000502:physiology*; D014176:Protein Biosynthesis; D051076:Proto-Oncogene Proteins c-fyn / Q000502:physiology*
chemical_list: C560488:Mobp protein, mouse; D009185:Myelin Proteins; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn
keywords: Fyn; MBP; MOBP; Morphological differentiation; Myelin; Oligodendrocyte; Translational regulation