title: Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung.
abstract: NiO nanoparticles were administered to rat lungs via intratracheal instillation or inhalation. During pulmonary toxicity caused by NiO nanoparticles, the induction of oxidative stress is a major factor. Both intratracheal instillation and inhalation of NiO nanoparticles induced pulmonary oxidative stress. The oxidative stress response protein, heme oxygenase-1 (HO-1), was induced by the administration of NiO nanoparticles at both the protein and gene expression level. Additionally, certain oxidative-stress markers in the lung, such as 8-iso-prostaglandin F2α, thioredoxin, and inducible nitric oxide synthase were increased. Furthermore, the concentration of myeloperoxidase (MPO) in the lung was also increased by the administration of NiO nanoparticles. When the amount of NiO in the lung is similar, the responses against pulmonary oxidative stress of intratracheal instillation and inhalation are also similar. However, the state of pulmonary oxidative stress in the early phase was different between intratracheal instillation and inhalation, even if the amount of NiO in the lung was similar. Inhalation causes milder oxidative stress than that caused by intratracheal instillation. On evaluation of the nanoparticle-induced pulmonary oxidative stress in the early phase, we should understand the different states of oxidative stress induced by intratracheal instillation and inhalation.
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu 761-0301, Kagawa, Japan. masa-horie@aist.go.jp.|Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. masa-horie@aist.go.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Horie
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. y-yoshiura@med.uoeh-u.ac.jp.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yoshiura
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. h-izumi@med.uoeh-u.ac.jp.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Izumi
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. toyabu@med.uoeh-u.ac.jp.
authors.forename: Takako
authors.initials: T
authors.lastname: Oyabu
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. t-tomonaga@med.uoeh-u.ac.jp.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Tomonaga
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. okadatakami@med.uoeh-u.ac.jp.
authors.forename: Takami
authors.initials: T
authors.lastname: Okada
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. leebw401@med.uoeh-u.ac.jp.
authors.forename: Byeong-Woo
authors.initials: BW
authors.lastname: Lee
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. tmyojo@med.uoeh-u.ac.jp.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Myojo
authors.affiliation: Department of Chemical Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, Japan. mkubo@hiroshima-u.ac.jp.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kubo
authors.affiliation: Department of Chemical Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, Japan. smd@hiroshima-u.ac.jp.
authors.forename: Manabu
authors.initials: M
authors.lastname: Shimada
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan. yasuom@med.uoeh-u.ac.jp.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morimoto
keywords: inhalation; intratracheal instillation; nanoparticle; nickel oxide; oxidative stress