title: Antioxidant and Anti-Inflammatory Properties of an Extract Rich in Polysaccharides of the Mushroom Polyporus dermoporus.
abstract: Polyporus dermoporus mushroom, native to Brazil, is produced under natural conditions in the unexplored reserve of Mata da Estrela-Rio Grande do Norte-RN. These mushrooms were delipidated with chloroform:methanol (2:1 v/v), extracted with water at 100 °C, and fractionated with ethanol (one and three volumes) and then centrifuged. The ethanol precipitation showed a high total sugar level of 64.8% and 1% of protein. This precipitate contained a high glucan level, characterized by chemical methods and by NMR of (13)C and ¹H and spectroscopy. The (13)C NMR spectrum of these mushroom extracts showed the presence of β-glucose by a signal at 103.25 ppm. Studies with these glucans were made to elucidate antioxidant and anti-inflammatory activities. This extract of glucans inhibited the lipid peroxidation (42.9%) and superoxide radicals (83.3%) at 67 μg/mL. However, the inhibition of hydroxyl radical by the extract of this mushroom was 96% at 267 μg/mL. The action of this extract on induced pleurisy showed a 92.5% and 68.7% reduction in polymorphonuclears cells and nitric oxide, respectively, at 30 mg/kg. The glucans reduced the croton oil-induced ear edema by 65.6% at 30 mg/kg.
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59072-940, Natal, Brazil. cmpguerra@hotmail.com.
authors.forename: Celina Maria P Guerra
authors.initials: CM
authors.lastname: Dore
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59072-940, Natal, Brazil. moniquegabi@gmail.com.
authors.forename: Monique Gabriela das Chagas F
authors.initials: MG
authors.lastname: Alves
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59072-940, Natal, Brazil. gloria_bios@hotmail.com.
authors.forename: Maria da Glória L
authors.initials: Mda G
authors.lastname: Santos
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59072-940, Natal, Brazil. leo_augustopdf@hotmail.com.
authors.forename: Leonardo Augusto R
authors.initials: LA
authors.lastname: de Souza
authors.affiliation: Laboratory of Mycology, Department of Botany, Ecology and Zoology, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova 59078-970, Natal, Brazil. baseia@cb.ufrn.br.
authors.forename: Iuri Goulart
authors.initials: IG
authors.lastname: Baseia
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59072-940, Natal, Brazil. eddaleite@cb.ufrn.br.
authors.forename: Edda Lisboa
authors.initials: EL
authors.lastname: Leite
keywords: Polyporus dermoporus; anti-inflammatory effect; antioxidant activities; glucan-protein; mushroom; polysaccharides