title: Investigation on host susceptibility of Tibetan pig to infection of porcine reproductive and respiratory syndrome virus through viral challenge study.
abstract: Previous reports showed that infection of porcine reproductive and respiratory syndrome virus (PRRSV) stimulated a variable host response and pig susceptibility to PRRSV was largely dependent on its genetic composition. In the present study, host susceptibility of Tibetan pig to PRRSV was compared with other two pig breeds, ZangMei black and Large White, by challenge of them with highly pathogenic PRRSV (HP-PRRSV). In the first challenge test, each eight piglets of the three breeds were inoculated with HP-PRRSV and clinical symptoms, viremia and animal mortality were examined up to 28 days post inoculation (DPI). In the secondary pathological study, each twelve piglets of the three breeds were challenged and three pigs of each breed were sacrificed on 4, 7, and 14 DPI for examination of gross damage and lung microscopic lesions. The results showed that no typical clinical signs such as cough, diarrhea and high fever were observed in challenged Tibetan pigs, which however all occurred in Large White accompanied with ∼40% mortality (3/8). In addition, a significant low and short viremia was detected specifically in Tibetan pigs. Based on histopathological analysis of lung sections, a mild to moderate interstitial pneumonia in Tibetan pigs and a much severe pneumonia in Large White were identified on 7-14 DPI. In summary, the study demonstrated that three genetically different pig breeds exhibited a differential host susceptibility to HP-PRRSV and Tibetan pig was much less susceptible to the virus in the three tested pig breeds.
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China; Sichuan Animal Science Academy, Chengdu, PR China.
authors.forename: Runmin
authors.initials: R
authors.lastname: Kang
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China.
authors.forename: Gaosheng
authors.initials: G
authors.lastname: Ji
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Sichuan Animal Science Academy, Chengdu, PR China.
authors.forename: Xuebing
authors.initials: X
authors.lastname: Lv
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China.
authors.forename: Mengyun
authors.initials: M
authors.lastname: Ge
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China.
authors.forename: Qingzhou
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China. Electronic address: whongning@163.com.
authors.forename: Hongning
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, Sichuan University, Chengdu, PR China. Electronic address: zengfanya@scu.edu.cn.
authors.forename: Fanya
authors.initials: F
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001831:Body Temperature; D001947:Breeding; D016207:Cytokines / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology; D020022:Genetic Predisposition to Disease; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D013552:Swine / Q000145:classification* / Q000235:genetics*; D019562:Viral Load; D015430:Weight Gain
chemical_list: D016207:Cytokines
keywords: Challenge; Highly pathogenic porcine reproductive and respiratory syndrome virus; Host susceptibility; Sus scrofa; Tibetan pig