title: Development of IgG Mediated Antibody Dependent Cell-mediated Cytotoxicity (ADCC) in the Serum and Genital Mucosa of HIV Seroconverters.
abstract: BACKGROUND
  We measured antibody-dependent cell mediated cytotoxicity (ADCC) activity in serum and genital fluids of heterosexually exposed women during HIV seroconversion.
  
  
  METHODS
  Plasma and cervico-vaginal lavage (CVL) fluid from 11 seroconverters (SC) were analyzed biannually from one year pre- to 6 year post-seroconversion using a51Cr-release assay to measure HIV-1 gp120 specific ADCC.
  
  
  RESULTS
  No SC had significant HIV specific CVL ADCC activity before seroconversion or until 1.5 yr after seroconversion. One individual had a %Specific Release (SR) of 25.4 at 2 years, 26.7 at 3 years and 21.0 at 4 years after seroconversion in CVL. Another sample had 4.7% SR at 2 years, 5.3 at 3 years, 10.9 at 4 years, and 8.4 at 5 years after seroconversion in CVL. A third had no activity until 17% SR 5 years after seroconversion in CVL. A fourth showed activity of 36.5% SR at 6.5 years after seroconversion. Seven women had no ADCC activity in their CVL. Paired serum samples showed HIV specific ADCC activity prior to the appearance of CVL ADCC activity.
  
  
  CONCLUSIONS
  HIV specific ADCC activity in CVL rose 2 years after seroconversion; ADCC was present in the serum prior to this time. These data suggest that genital tract ADCC activity is not present until well after acute infection.
authors.affiliation: CORE Center, Cook County Health and Hospitals System, Chicago, IL 60612, USA; Division of Infectious Diseases, Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Mariam
authors.initials: M
authors.lastname: Aziz
authors.affiliation: Department of Immunology/Microbiology Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Fareeha
authors.initials: F
authors.lastname: Mahmood
authors.affiliation: Department of Immunology/Microbiology Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Mariana
authors.initials: M
authors.lastname: Mata
authors.affiliation: SUNY Downstate Medical Center, Brooklyn, NY, 11203, USA.
authors.forename: Helen G
authors.initials: HG
authors.lastname: Durkin
authors.affiliation: Georgetown University, Washington, DC, 20057, USA.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Liu
authors.affiliation: University of California, San Francisco, CA, 94110 USA.
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Greenblatt
authors.affiliation: University of Southern California Norris Hospital, Los Angeles, CA, 90033 USA.
authors.forename: Marek
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Elizabeth T
authors.initials: ET
authors.lastname: Golub
authors.affiliation: Montefiore Medical Center, Bronx, NY, 10467 USA.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Anastos
authors.affiliation: CORE Center, Cook County Health and Hospitals System, Chicago, IL 60612, USA; Division of Infectious Diseases, Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Audrey L
authors.initials: AL
authors.lastname: French
authors.affiliation: Department of Immunology/Microbiology Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Linda L
authors.initials: LL
authors.lastname: Baum
keywords: Antibody dependent cellular cytotoxicity; HIV; Seroconverters; Women