title: Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
abstract: Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging. This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles. This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers. In addition, the diameter of the composites can be tuned by adjusting the flow on the microfluidic chip. The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data. The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles. The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability. Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan.
authors.forename: Chih-Hui
authors.initials: CH
authors.lastname: Yang
authors.affiliation: The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan; Department of Chinese Medicine, E-Da Hospital, Kaohsiung, Taiwan.
authors.forename: Lung-Shuo
authors.initials: LS
authors.lastname: Wang
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan.
authors.forename: Szu-Yu
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan.
authors.forename: Mao-Chen
authors.initials: MC
authors.lastname: Huang
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan.
authors.forename: Ya-Hua
authors.initials: YH
authors.lastname: Li
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan.
authors.forename: Yun-Chul
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan.
authors.forename: Pei-Fan
authors.initials: PF
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Taiwan. Electronic address: shawjf@isu.edu.tw.
authors.forename: Jei-Fu
authors.initials: JF
authors.lastname: Shaw
authors.affiliation: The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Taiwan. Electronic address: huangks@isu.edu.tw.
authors.forename: Keng-Shiang
authors.initials: KS
authors.lastname: Huang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010316:Particle Size; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D048271:Chitosan
keywords: Antibacterial; Chitosan; Microfluidic; Nanoparticles; Silver; Synthesis