title: DELLA proteins physically interact with CONSTANS to regulate flowering under long days in Arabidopsis.
abstract: Proper timing of flowering is essential for reproduction of plants. Although it is well known that both light and gibberellin (GA) signaling play critical roles in promoting flowering in Arabidopsis thaliana, whether and how they are integrated to regulate flowering remain largely unknown. Here, we show through biochemical studies that DELLA proteins physically interact with CONSTANS (CO). Furthermore, the interaction of CO with NF-YB2 is inhibited by the DELLA protein, RGA. Our findings suggest that regulation of flowering by GA signaling in leaves under long days is mediated, at least in part, through repression of DELLA proteins on CO, providing a molecular link between DELLA proteins, key components in GA signaling pathway, and CO, a critical flowering activator in photoperiod signaling pathway.
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agriculture and Biology, Shanghai Jiaotong University, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agriculture and Biology, Shanghai Jiaotong University, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agriculture and Biology, Shanghai Jiaotong University, China.
authors.forename: Peng-Bo
authors.initials: PB
authors.lastname: Xu
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agriculture and Biology, Shanghai Jiaotong University, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Du
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agriculture and Biology, Shanghai Jiaotong University, China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Lian
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005875:Gibberellins / Q000378:metabolism*; D047468:Immunoprecipitation; D017440:Photoperiod; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; C092776:CONSTANS protein, Arabidopsis; D004268:DNA-Binding Proteins; C109555:GAI protein, Arabidopsis; D005875:Gibberellins; C000609478:NF-YB2 protein, Arabidopsis; C508468:RGA protein, Arabidopsis; D012097:Repressor Proteins; D014157:Transcription Factors
keywords: CONSTANS; DELLA proteins; Nuclear Factor Y; flowering; photoperiodic