title: Gene Expressing and sRNA Sequencing Show That Gene Differentiation Associates with a Yellow Acer palmatum Mutant Leaf in Different Light Conditions.
abstract: Acer palmatum Thunb., like other maples, is a widely ornamental-use small woody tree for leaf shapes and colors. Interestingly, we found a yellow-leaves mutant "Jingling Huangfeng" turned to green when grown in shade or low-density light condition. In order to study the potential mechanism, we performed high-throughput sequencing and obtained 1,082 DEGs in leaves grown in different light conditions that result in A. palmatum significant morphological and physiological changes. A total of 989 DEGs were annotated and clustered, of which many DEGs were found associating with the photosynthesis activity and pigment synthesis. The expression of CHS and FDR gene was higher while the expression of FLS gene was lower in full-sunlight condition; this may cause more colorful substance like chalcone and anthocyanin that were produced in full-light condition, thus turning the foliage to yellow. Moreover, this is the first available miRNA collection which contains 67 miRNAs of A. palmatum, including 46 conserved miRNAs and 21 novel miRNAs. To get better understanding of which pathways these miRNAs involved, 102 Unigenes were found to be potential targets of them. These results will provide valuable genetic resources for further study on the molecular mechanisms of Acer palmatum leaf coloration.
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, No. 50 Zhonglingjie, Nanjing 210014, China.
authors.forename: Shu-Shun
authors.initials: SS
authors.lastname: Li
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, No. 50 Zhonglingjie, Nanjing 210014, China.
authors.forename: Qian-Zhong
authors.initials: QZ
authors.lastname: Li
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, No. 50 Zhonglingjie, Nanjing 210014, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Rong
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, No. 50 Zhonglingjie, Nanjing 210014, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tang
authors.affiliation: College of Biology and The Environment, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D031002:Acer / Q000235:genetics* / Q000254:growth & development; D002734:Chlorophyll / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008027:Light; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D010788:Photosynthesis; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs; D002734:Chlorophyll; D011494:Protein Kinases