title: An NF-κB--EphrinA5-Dependent Communication between NG2(+) Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.
abstract: Skeletal muscle growth immediately following birth is critical for proper body posture and locomotion. However, compared with embryogenesis and adulthood, the processes regulating the maturation of neonatal muscles is considerably less clear. Studies in the 1960s predicted that neonatal muscle growth results from nuclear accretion of myoblasts preferentially at the tips of myofibers. Remarkably, little information has been added since then to resolve how myoblasts migrate to the ends of fibers. Here, we provide insight into this process by revealing a unique NF-κB-dependent communication between NG2(+) interstitial cells and myoblasts. NF-κB in NG2(+) cells promotes myoblast migration to the tips of myofibers through cell-cell contact. This occurs through expression of ephrinA5 from NG2(+) cells, which we further deduce is an NF-κB target gene. Together, these results suggest that NF-κB plays an important role in the development of newborn muscles to ensure proper myoblast migration for fiber growth.
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Medical Center, Columbus, OH 43210, USA; Center for Muscle Health and Neuromuscular Disorders, The Ohio State University Medical Center, Columbus, OH 43210, USA; Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University Medical Center, Columbus, OH 43210, USA; The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: Jin-Mo
authors.initials: JM
authors.lastname: Gu
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Medical Center, Columbus, OH 43210, USA; The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Wang
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Medical Center, Columbus, OH 43210, USA; Center for Muscle Health and Neuromuscular Disorders, The Ohio State University Medical Center, Columbus, OH 43210, USA; The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Peterson
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Medical Center, Columbus, OH 43210, USA; Center for Muscle Health and Neuromuscular Disorders, The Ohio State University Medical Center, Columbus, OH 43210, USA; Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University Medical Center, Columbus, OH 43210, USA; The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Shintaku
authors.affiliation: The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: Sandya
authors.initials: S
authors.lastname: Liyanarachchi
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Medical Center, Columbus, OH 43210, USA; The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Coppola
authors.affiliation: Nationwide Children's Hospital, Columbus, OH 43205, USA.
authors.forename: Ashley E
authors.initials: AE
authors.lastname: Frakes
authors.affiliation: Nationwide Children's Hospital, Columbus, OH 43205, USA.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kaspar
authors.affiliation: Division of Biology and Bond Life Sciences Center, University of Missouri, Columbia, MO 65203, USA.
authors.forename: Dawn D
authors.initials: DD
authors.lastname: Cornelison
authors.affiliation: Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Medical Center, Columbus, OH 43210, USA; Center for Muscle Health and Neuromuscular Disorders, The Ohio State University Medical Center, Columbus, OH 43210, USA; Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University Medical Center, Columbus, OH 43210, USA; The Ohio State University Medical Center, Columbus, OH 43210, USA. Electronic address: denis.guttridge@osumc.edu.
authors.forename: Denis C
authors.initials: DC
authors.lastname: Guttridge
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000941:Antigens / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D036386:Ephrin-A5 / Q000378:metabolism*; D008297:Male; D018345:Mice, Knockout; D008822:Mice, Transgenic; D024510:Muscle Development / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D036386:Ephrin-A5; D016328:NF-kappa B; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4
keywords: NF-κB; development; ephrinA5; migration; myoblast; skeletal muscle