title: Src family tyrosine kinase inhibitors suppress Nav1.1 expression in cultured rat spiral ganglion neurons.
abstract: Src family kinases regulate neuronal voltage-gated Na(+) channels, which generate action potentials. The mechanisms of action, however, remain poorly understood. The aim of the present study was to further elucidate the effects of Src family kinases on Nav1.1 mRNA and protein expression in spiral ganglion neurons. Immunofluorescence staining techniques detected Nav1.1 expression in the spiral ganglion neurons. Additionally, quantitative PCR and western blot techniques were used to analyze Nav1.1 mRNA and protein expression, respectively, in spiral ganglion neurons following exposure to Src family kinase inhibitors PP2 (1 and 10 μM) and SU6656 (0.1 and 1 μM) for different lengths of time (6 and 24 h). In the spiral ganglion neurons, Nav1.1 protein expression was detected in the somas and axons. The Src family kinase inhibitors PP2 and SU6665 significantly decreased Nav1.1 mRNA and protein expression (p < 0.05), respectively, in the spiral ganglion neurons, and changes in expression were not dependent on time or dose (p > 0.05).
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Qingjiao
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Tingjia
authors.initials: T
authors.lastname: Wei
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. ymsu2@126.com.
authors.forename: Jiping
authors.initials: J
authors.lastname: Su
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001831:Body Temperature; D002478:Cells, Cultured; D003051:Cochlea / Q000033:anatomy & histology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007211:Indoles / Q000494:pharmacology; D062550:NAV1.1 Voltage-Gated Sodium Channel / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D011720:Pyrazoles / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000166:cytology*; D013449:Sulfonamides / Q000494:pharmacology; D013997:Time Factors; D019061:src-Family Kinases / Q000037:antagonists & inhibitors*
chemical_list: C509621:4-amino-7-phenylpyrazol(3,4-d)pyrimidine; C412373:AG 1879; D004791:Enzyme Inhibitors; D007211:Indoles; D062550:NAV1.1 Voltage-Gated Sodium Channel; D011720:Pyrazoles; D011743:Pyrimidines; D012333:RNA, Messenger; C416927:SU 6656; C568249:Scn1a protein, mouse; D013449:Sulfonamides; D019061:src-Family Kinases
keywords: Nav1.1; Quantitative PCR; Spiral ganglion neurons; Src family kinases; Western blot