title: Investigation of six bioactive anthraquinones in slimming tea by accelerated solvent extraction and high performance capillary electrophoresis with diode-array detection.
abstract: A rapid and effective method for effective separation and rapid simultaneous determination of six bioactive anthraquinones by capillary zone electrophoresis was developed. An accelerated solvent extraction procedure was used for the extraction of anthraquinones from slimming tea. Under the optimized conditions, the effective separation of six anthraquinones was achieved within 8 min. Good linearity was achieved, with a correlation coefficient (r) of ⩾ 0.999. The limit of detection ranged from 0.33 to 1.40 μg mL(-1). The intra- and inter-day relative standard deviation (RSD) of the six analytes was in the range of 2.3-3.9% and 3.2-4.9%, respectively. The average recovery of the six analytes from real tea samples was in the range of 86.15-98.30% with the RSD of 1.04-4.99%. The developed and validated method has speediness, high sensitivity, recovery and precision, and can be applied for the quality control of slimming tea.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Shuxuan
authors.initials: S
authors.lastname: Liang
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China. Electronic address: hanwen@hbu.edu.cn.
authors.forename: Hanwen
authors.initials: H
authors.lastname: Sun
mesh_terms: D000880:Anthraquinones / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D012997:Solvents / Q000737:chemistry; D013662:Tea / Q000737:chemistry*; D013696:Temperature
chemical_list: D000880:Anthraquinones; D010936:Plant Extracts; D012997:Solvents; D013662:Tea
keywords: Accelerated solvent extraction; Anthraquinones; Capillary zone electrophoresis; Slimming tea