title: Enantio-alteration of gene transcription associated with bioconcentration in adult zebrafish (Danio rerio) exposed to chiral PCB149.
abstract: Enantioselective enrichment of chiral PCB149 (2,2',3,4',5',6-hexachlorobiphenyl) was analysed in adult zebrafish (Danio rerio) exposed to the racemate, (-)-PCB149, and (+)-PCB149. Greater enrichment of (-)-PCB149 compared to (+) PCB149 was observed following 0.5 ng/L exposure; however, as the exposure time and concentration increased, racemic enrichment was observed in adult fish exposed to the racemate. No biotransformation between the two isomers was observed in fish exposed to single enantiomers. When zebrafish were exposed to different forms of chiral PCB149, enantioselective expression of genes associated with polychlorinated biphenyls (PCBs) was observed in brain and liver tissues and enantioselective correlations between bioconcentration and target gene expression levels were observed in brain and liver tissues. The strong positive correlations between expression levels of target genes (alox5a and alox12) and PCB149 bioconcentration suggest that prolonged exposure to the racemate of chiral PCB149 may result in inflammation-associated diseases. Prolonged exposure to (-)-PCB149 may also affect metabolic pathways such as dehydrogenation and methylation in the brain tissues of adult zebrafish. Hepatic expression levels of genes related to the antioxidant system were significantly negatively correlated with bioconcentration following exposure to (+)-PCB149.
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.|College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chai
authors.affiliation: College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Cui
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Pengqian
authors.initials: P
authors.lastname: Mu
authors.affiliation: College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Xu
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.affiliation: College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Chengju
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D014158:Transcription, Genetic; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; C120129:2,2',3,4',5',6-hexachlorobiphenyl; D011078:Polychlorinated Biphenyls