title: Magnaporthe oryzae-Secreted Protein MSP1 Induces Cell Death and Elicits Defense Responses in Rice.
abstract: The Magnaporthe oryzae snodprot1 homolog (MSP1), secreted by M. oryzae, is a cerato-platanin family protein. msp1-knockout mutants have reduced virulence on barley leaves, indicating that MSP1 is required for the pathogenicity of rice blast fungus. To investigate the functional roles of MSP1 and its downstream signaling in rice, recombinant MSP1 was produced in Escherichia coli and was assayed for its functionality. Application of MSP1 triggered cell death and elicited defense responses in rice. MSP1 also induced H2O2 production and autophagic cell death in both suspension-cultured cells and rice leaves. One or more protein kinases triggered cell death, jasmonic acid and abscisic acid enhanced cell death, while salicylic acid suppressed it. We demonstrated that the secretion of MSP1 into the apoplast is a prerequisite for triggering cell death and activating defense-related gene expression. Furthermore, pretreatment of rice with a sublethal MSP1 concentration potentiated resistance to the pathogen. Taken together, our results showed that MSP1 induces a high degree of cell death in plants, which might be essential for its virulence. Moreover, rice can recognize MSP1, resulting in the induction of pathogen-associated molecular pattern-triggered immunity.
authors.affiliation: 1 Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea;|2 Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, Cologne, 50829, Germany;
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 2 Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, Cologne, 50829, Germany;|3 Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, Korea;
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.affiliation: 1 Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea;
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.affiliation: 2 Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, Cologne, 50829, Germany;
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tsuda
authors.affiliation: 4 Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea; and.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: 5 Korean Lichen Research Institute, Sunchon National University, Suncheon, South Korea.
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: 4 Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea; and.
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: 1 Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea;|3 Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, Korea;
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
mesh_terms: D017360:Arabidopsis / Q000276:immunology / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure; D001343:Autophagy / Q000187:drug effects; D003517:Cyclopentanes / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D020082:Magnaporthe / Q000472:pathogenicity / Q000502:physiology*; D008954:Models, Biological; D012275:Oryza / Q000276:immunology / Q000382:microbiology* / Q000502:physiology / Q000648:ultrastructure; D054883:Oxylipins / Q000494:pharmacology; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D057865:Plant Immunity; D018515:Plant Leaves / Q000276:immunology / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins; D020156:Salicylic Acid / Q000494:pharmacology; D014026:Nicotiana / Q000276:immunology / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure
chemical_list: D003517:Cyclopentanes; D005656:Fungal Proteins; D054883:Oxylipins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D010937:Plant Growth Regulators; D011994:Recombinant Proteins; C011006:jasmonic acid; D006861:Hydrogen Peroxide; D020156:Salicylic Acid