title: Genome-Wide Analysis and Heavy Metal-Induced Expression Profiling of the HMA Gene Family in Populus trichocarpa.
abstract: The heavy metal ATPase (HMA) family plays an important role in transition metal transport in plants. However, this gene family has not been extensively studied in Populus trichocarpa. We identified 17 HMA genes in P. trichocarpa (PtHMAs), of which PtHMA1-PtHMA4 belonged to the zinc (Zn)/cobalt (Co)/cadmium (Cd)/lead (Pb) subgroup, and PtHMA5-PtHMA8 were members of the copper (Cu)/silver (Ag) subgroup. Most of the genes were localized to chromosomes I and III. Gene structure, gene chromosomal location, and synteny analyses of PtHMAs indicated that tandem and segmental duplications likely contributed to the expansion and evolution of the PtHMAs. Most of the HMA genes contained abiotic stress-related cis-elements. Tissue-specific expression of PtHMA genes showed that PtHMA1 and PtHMA4 had relatively high expression levels in the leaves, whereas Cu/Ag subgroup (PtHMA5.1- PtHMA8) genes were upregulated in the roots. High concentrations of Cu, Ag, Zn, Cd, Co, Pb, and Mn differentially regulated the expression of PtHMAs in various tissues. The preliminary results of the present study generated basic information on the HMA family of Populus that may serve as foundation for future functional studies.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: Library of Northeast Forestry University Harbin, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Quangang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Zhanchao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Hanzeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Haimei
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Li
keywords: HMA gene; P1B-type ATPases; Populus; heavy metal stress; phytoremediation