title: Identification of miRNAs and differentially expressed genes in early phase non-small cell lung cancer.
abstract: To explore the potential therapeutic targets of early‑stage non-small cell lung cancer (NSCLC), gene microarray analysis was conducted. The microarray data of NSCLC in stage IA, IB, IIA, and IIB (GSE50081), were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) in IB vs. IA, IIA vs. IB, IIB vs. IIA were screened out via R. ToppGene Suite was used to get the enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the DEGs. The GeneCoDis3 database and Cytoscape software were used to construct the transcriptional regulatory network. In total, 25, 17 and 14 DEGs were identified in IB vs. IA, IIA vs. IB, IIB vs. IIA of NSCLC, respectively. Some GO terms and pathways (e.g., extracellular space, alveolar lamellar body, bioactivation via cytochrome P450 pathway) were found significantly enriched in DEGs. Genes S100P, ALOX15B, CCL11, NLRP2, SERPINA3, FoxO4 and hsa-miR-491 may play important roles in the development of early-stage NSCLC. Thus, by bioinformatics analysis the key genes and biological processes involving in the development of early-stage NSCLC could be established, providing more potential references for the therapeutic targets.
authors.affiliation: Department of Internal Medicine-Oncology, Cangzhou Central Hospital, Cangzhou Municipality, Hebei 061000, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Tian
authors.affiliation: Renqiu People's Hospital, Renqiu Municipality, Hebei 062550, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics Department II, Cangzhou Central Hospital, Cangzhou Municipality, Hebei 061000, P.R. China.
authors.forename: Baojing
authors.initials: B
authors.lastname: Pei
authors.affiliation: Hejian People's Hospital, Hejian Municipality, Cangzhou Municipality, Hebei 062450, P.R. China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Hejian People's Hospital, Hejian Municipality, Cangzhou Municipality, Hebei 062450, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Cangxian County Dulin Central Hospital, Cangxian County, Cangzhou Municipality, Hebei 061000, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs