title: Optimization of growth medium for Sporosarcina pasteurii in bio-based cement pastes to mitigate delay in hydration kinetics.
abstract: Microbial-induced calcium carbonate precipitation has been identified as a novel method to improve durability and remediate cracks in concrete. One way to introduce microorganisms to concrete is by replacing the mixing water with a bacterial culture in nutrient medium. In the literature, yeast extract often has been used as a carbon source for this application; however, severe retardation of hydration kinetics has been observed when yeast extract is added to cement. This study investigates the suitability of alternative carbon sources to replace yeast extract for microbial-induced calcium carbonate precipitation in cement-based materials. A combination of meat extract and sodium acetate was identified as a suitable replacement in growth medium for Sporosarcina pasteurii; this alternative growth medium reduced retardation by 75 % (as compared to yeast extract) without compromising bacterial growth, urea hydrolysis, cell zeta potential, and ability to promote calcium carbonate formation.
authors.affiliation: Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin, 301 East Dean Keeton Street, Stop C1700, Austin, TX, 78712, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Williams
authors.affiliation: Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin, 301 East Dean Keeton Street, Stop C1700, Austin, TX, 78712, USA.
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Kirisits
authors.affiliation: Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin, 301 East Dean Keeton Street, Stop C1700, Austin, TX, 78712, USA. rferron@mail.utexas.edu.
authors.forename: Raissa Douglas
authors.initials: RD
authors.lastname: Ferron
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry* / Q000378:metabolism*; D003252:Construction Materials / Q000032:analysis / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D003890:Desiccation; D006868:Hydrolysis; D007700:Kinetics; D058644:Sporosarcina / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D014508:Urea / Q000378:metabolism
chemical_list: D003470:Culture Media; D014508:Urea; D002119:Calcium Carbonate
keywords: Biomineralization; Calcium carbonate; Hydration; Meat extract; Yeast extract