title: "Cold training" affects rat liver responses to continuous cold exposure.
abstract: Continuous exposure of homeothermic animals to low environmental temperatures elicits physiological adaptations necessary for animal survival, which are associated to higher generation of pro-oxidants in thermogenic tissues. It is not known whether intermittent cold exposure (cold training) is able to affect tissue responses to continuous cold exposure. Therefore, we investigated whether rat liver responses to continuous cold exposure of 2 days are modified by cold training (1h daily for 5 days per week for 3 consecutive weeks). Continuous cold increased liver oxidative metabolism by increasing tissue content of mitochondrial proteins and mitochondrial aerobic capacity. Cold training did not affect such parameters, but attenuated or prevented the changes elicited by continuous cold exposure. Two-day cold exposure increased lipid hydroperoxide and protein-bound carbonyl levels in homogenates and mitochondria, whereas cold training decreased such effects although it decreased only homogenate protein damage in control rats. The activities of the antioxidant enzymes GPX and GR and H2O2 production were increased by continuous cold exposure. Despite the increase in GPX and GR activities, livers from cold-exposed rats showed increased susceptibility to in vitro oxidative challenge. Such cold effects were decreased by cold training, which in control rats reduced only H2O2 production and susceptibility to stress. The changes of PGC-1, NRF-1, and NRF-2 expression levels were consistent with those induced by cold exposure and cold training in mitochondrial protein content and antioxidant enzyme activities. However, the mechanisms by which cold training attenuates the effects of the continuous cold exposure remain to be elucidated.
authors.affiliation: Department of Biology, University of Naples, I-80126 Naples, Italy. Electronic address: venditti@unina.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Venditti
authors.affiliation: Department of Biology, University of Naples, I-80126 Naples, Italy.
authors.forename: Gaetana
authors.initials: G
authors.lastname: Napolitano
authors.affiliation: Department of Biology, University of Naples, I-80126 Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Barone
authors.affiliation: Department of Biology, University of Naples, I-80126 Naples, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Di Meo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003080:Cold Temperature; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008930:Mitochondria, Liver / Q000235:genetics* / Q000378:metabolism*; D051265:NF-E2-Related Factor 1 / Q000096:biosynthesis / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics*; D010101:Oxygen Consumption / Q000235:genetics; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D051265:NF-E2-Related Factor 1; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat
keywords: Cold exposure; Mitochondria; Oxidative damage; Oxidative metabolism; Rat liver