title: A microfluidic indirect competitive immunoassay for multiple and sensitive detection of testosterone in serum and urine.
abstract: We demonstrate a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay (MIC) for multiple, sensitive, reliable and rapid detection of testosterone in human serum and urine samples. As MIC can detect biomarkers in a cost-effective and easy-to-operate manner, it may have great potential for clinical diagnosis and point-of-care testing (POCT).
authors.affiliation: School of Chemical Engineering and Material Science, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Wang
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Binfeng
authors.initials: B
authors.lastname: Hu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Ruitao
authors.initials: R
authors.lastname: Cha
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002417:Cattle; D006041:Goats; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013739:Testosterone / Q000097:blood* / Q000652:urine*
chemical_list: D012710:Serum Albumin, Bovine; D013739:Testosterone; D008165:Luminol; D006735:Horseradish Peroxidase