title: Mutagenic Potential ofBos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description.
abstract: Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechanisms of BPV E6 oncoprotein per se remain unknown. This study aimed to evaluate the mutagenic potential of Bos taurus papillomavirus type 1 (BPV-1) E6 recombinant oncoprotein by the cytokinesis-block micronucleus assay (CBMNA) and comet assay (CA). Peripheral blood samples of five calves were collected. Samples were subjected to molecular diagnosis, which did not reveal presence of BPV sequences. Samples were treated with 1 μg/mL of BPV-1 E6 oncoprotein and 50 μg/mL of cyclophosphamide (positive control). Negative controls were not submitted to any treatment. The samples were submitted to the CBMNA and CA. The results showed that BPV E6 oncoprotein induces clastogenesis per se, which is indicative of genomic instability. These results allowed better understanding the mechanism of cancer promotion associated with the BPV E6 oncoprotein and revealed that this oncoprotein can induce carcinogenesis per se. E6 recombinant oncoprotein has been suggested as a possible vaccine candidate. Results pointed out that BPV E6 recombinant oncoprotein modifications are required to use it as vaccine.
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil; Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), 05508-900 São Paulo, SP, Brazil.
authors.forename: Rodrigo Pinheiro
authors.initials: RP
authors.lastname: Araldi
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil; Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), 05508-900 São Paulo, SP, Brazil.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Mazzuchelli-de-Souza
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Diego Grando
authors.initials: DG
authors.lastname: Modolo
authors.affiliation: Laboratório de Biologia Molecular, Genética e Mutagênese, Departamento de Biologia, Faculdade de Ciências e Letras de Assis (FCLA), Universidade Estadual Paulista "Júlio de Mesquista Filho" (UNESP), 19806-900 Assis, SP, Brazil.
authors.forename: Edislane Barreiros
authors.initials: EB
authors.lastname: de Souza
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Thatiana Corrêa
authors.initials: TC
authors.lastname: de Melo
authors.affiliation: Laboratório de Fisiopatologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Diva Denelle
authors.initials: DD
authors.lastname: Spadacci-Morena
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Roberta Fiusa
authors.initials: RF
authors.lastname: Magnelli
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Márcio Augusto Caldas Rocha
authors.initials: MA
authors.lastname: de Carvalho
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Paulo Luis
authors.initials: PL
authors.lastname: de Sá Júnior
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Rodrigo Franco
authors.initials: RF
authors.lastname: de Carvalho
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Willy
authors.initials: W
authors.lastname: Beçak
authors.affiliation: Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
authors.forename: Rita de Cassia
authors.initials: Rde C
authors.lastname: Stocco
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000472:pathogenicity; D063646:Carcinogenesis / Q000235:genetics; D002417:Cattle; D002460:Cell Line; D003520:Cyclophosphamide / Q000008:administration & dosage; D042822:Genomic Instability / Q000187:drug effects; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000008:administration & dosage / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics*
chemical_list: D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; C059269:protein E6, Bovine papillomavirus; D003520:Cyclophosphamide