title: Nitric Oxide Alleviated Arsenic Toxicity by Modulation of Antioxidants and Thiol Metabolism in Rice (Oryza sativa L.).
abstract: Nitric oxide (NO) is a gaseous signaling molecule and has a profound impact on plant growth and development. It is reported to serve as pro oxidant as well as antioxidant in plant system. In the present study, we evaluated the protective role of NO against arsenate (As(V)) toxicity in rice plants. As(V) exposure has hampered the plant growth, reduced the chlorophyll content, and enhanced the oxidative stress, while the exogenous NO supplementation has reverted these symptoms. NO supplementation has reduced the arsenic (As) accumulation in root as well as shoot. NO supplementation to As(V) exposed plants has reduced the gene expression level of OsLsi1 and OsLsi2. As(V) stress significantly impacted thiol metabolism, it reduced GSH content and GSH/GSSG ratio, and enhanced the level of PCs. NO supplementation maintained the GSH/GSSG ratio and reduced the level of PCs. NO supplementation reverted As(V) induced iron deficiency in shoot and had significant impact of gene expression level of various iron transporters (OsYSL2, OsFRDL1, OsIRT1, and OsIRO2). Conclusively, exogenous application of NO could be advantageous against As(V) toxicity and could confer the tolerance to As(V) stress in rice.
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Amit P
authors.initials: AP
authors.lastname: Singh
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Garima
authors.initials: G
authors.lastname: Dixit
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Mishra
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Pradyumna K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Dwivedi
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Prabodh K
authors.initials: PK
authors.lastname: Trivedi
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Chakrabarty
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Shekhar
authors.initials: S
authors.lastname: Mallick
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Pandey
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts Amherst Amherst, MA, USA.
authors.forename: Om P
authors.initials: OP
authors.lastname: Dhankher
authors.affiliation: C.S.I.R.-National Botanical Research Institute Lucknow, India.
authors.forename: Rudra D
authors.initials: RD
authors.lastname: Tripathi
keywords: arsenate; arsenic transporter; iron transporter; nitric oxide; non-protein thiol; rice