title: Complete genome sequence of tobacco virus 1, a closterovirus from Nicotiana tabacum.
abstract: The complete genome sequence of a novel virus, provisionally named tobacco virus 1 (TV1), was determined, and this virus was identified in leaves of tobacco (Nicotiana tabacum) exhibiting leaf mosaic and yellowing symptoms in Anhui Province, China. The genome sequence of TV1 consists of 15,395 nucleotides with 61.6 % nucleotide sequence identity to mint virus 1 (MV1). Its genome organization is similar to that of MV1, containing nine open reading frames (ORFs) that potentially encode proteins with putative functions in virion assembly, cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis of the heat shock protein 70 homolog (HSP70h) placed TV1 alongside members of the genus Closterovirus in the family Closteroviridae. To our knowledge, this study is the first report of the complete genome sequence of a closterovirus identified in tobacco.
authors.affiliation: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Shuishui
authors.initials: S
authors.lastname: Qi
authors.affiliation: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China.
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Gao
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Ibukun A
authors.initials: IA
authors.lastname: Akinyemi
authors.affiliation: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Xu
authors.affiliation: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China. bgzhou@163.com.
authors.forename: Benguo
authors.initials: B
authors.lastname: Zhou
mesh_terms: D029381:Closteroviridae / Q000235:genetics*; D016679:Genome, Viral; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D014026:Nicotiana / Q000821:virology*
chemical_list: D012367:RNA, Viral