title: Preparation of Inert Polystyrene Latex Particles as MicroRNA Delivery Vectors by Surfactant-Free RAFT Emulsion Polymerization.
abstract: We present the preparation of 11 nm polyacrylamide-stabilized polystyrene latex particles for conjugation to a microRNA model by surfactant-free RAFT emulsion polymerization. Our synthetic strategy involved the preparation of amphiphilic polyacrylamide-block-polystyrene copolymers, which were able to self-assemble into polymeric micelles and "grow" into polystyrene latex particles. The surface of these sterically stabilized particles was postmodified with a disulfide-bearing linker for the attachment of the microRNA model, which can be released from the latex particles under reducing conditions. These nanoparticles offer the advantage of ease of preparation via a scaleable process, and the versatility of their synthesis makes them adaptable to a range of applications.
authors.affiliation: Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney , Building F11, Sydney, NSW 2006, Australia.
authors.forename: Cheuk Ka
authors.initials: CK
authors.lastname: Poon
authors.affiliation: Kolling Institute of Medical Research, Royal North Shore Hospital and The University of Sydney , St. Leonards, NSW 2065, Australia.
authors.forename: Owen
authors.initials: O
authors.lastname: Tang
authors.affiliation: Kolling Institute of Medical Research, Royal North Shore Hospital and The University of Sydney , St. Leonards, NSW 2065, Australia.
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Chen
authors.affiliation: Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney , Building F11, Sydney, NSW 2006, Australia.
authors.forename: Binh T T
authors.initials: BT
authors.lastname: Pham
authors.affiliation: Department of Chemistry, The University of Warwick , Coventry, CV4 7AL, United Kingdom.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gody
authors.affiliation: Kolling Institute of Medical Research, Royal North Shore Hospital and The University of Sydney , St. Leonards, NSW 2065, Australia.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Pollock
authors.affiliation: Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney , Building F11, Sydney, NSW 2006, Australia.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Hawkett
authors.affiliation: Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney , Building F11, Sydney, NSW 2006, Australia.|Department of Chemistry, The University of Warwick , Coventry, CV4 7AL, United Kingdom.|Faculty of Pharmacy and Pharmaceutical Sciences, Monash University , 381 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrier
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D065546:Drug Liberation; D004655:Emulsions / Q000737:chemistry; D007840:Latex / Q000737:chemistry*; D035683:MicroRNAs / Q000008:administration & dosage*; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D058105:Polymerization; D011137:Polystyrenes / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D004337:Drug Carriers; D004655:Emulsions; D007840:Latex; D035683:MicroRNAs; D011137:Polystyrenes; D013501:Surface-Active Agents; D000099166:Polyacrylamides