title: High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom.
abstract: Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.
authors.affiliation: Laboratório de Farmacologia, Instituto Butantan, São Paulo, Brazil.|Laboratório de Proteômica Funcional, Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo, ICT-UNIFESP, Campus de São José dos Campos, Rua Talim, 330, São Paulo, SP, 12231-280, Brazil.
authors.forename: Tarcísio
authors.initials: T
authors.lastname: Liberato
authors.affiliation: Laboratório de Farmacologia, Instituto Butantan, São Paulo, Brazil.
authors.forename: Lanfranco Ranieri Paolo
authors.initials: LRP
authors.lastname: Troncone
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, Brazil.
authors.forename: Edson T
authors.initials: ET
authors.lastname: Yamashiro
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, Brazil.
authors.forename: Solange M T
authors.initials: SMT
authors.lastname: Serrano
authors.affiliation: Laboratório de Proteômica Funcional, Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo, ICT-UNIFESP, Campus de São José dos Campos, Rua Talim, 330, São Paulo, SP, 12231-280, Brazil. andre.zelanis@unifesp.br.|Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, Brazil. andre.zelanis@unifesp.br.
authors.forename: André
authors.identifier: http://orcid.org/0000-0003-3066-6527
authors.initials: A
authors.lastname: Zelanis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*; D013111:Spider Venoms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D013112:Spiders / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D013111:Spider Venoms
keywords: Chitinase; Phoneutria nigriventer; Proteome; Spider venom