title: Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells.
abstract: Cyclic adenosine 3',5'-monophosphate (cAMP) is a recognized second messenger; however, knowledge of cAMP involvement in plant physiological processes originates primarily from pharmacological studies. To obtain direct evidence for cAMP function in plants, tobacco Bright Yellow-2 (BY-2) cells were transformed with the cAMP sponge, which is a genetically encoded tool that reduces cAMP availability. BY-2 cells expressing the cAMP sponge (cAS cells), showed low levels of free cAMP and exhibited growth inhibition that was not proportional to the cAMP sponge transcript level. Growth inhibition in cAS cells was closely related to the precocious inhibition of mitosis due to a delay in cell cycle progression. The cAMP deficiency also enhanced antioxidant systems. Remarkable changes occurred in the cAS proteomic profile compared with that of wild-type (WT) cells. Proteins involved in translation, cytoskeletal organization, and cell proliferation were down-regulated, whereas stress-related proteins were up-regulated in cAS cells. These results support the hypothesis that BY-2 cells sense cAMP deficiency as a stress condition. Finally, many proteasome subunits were differentially expressed in cAS cells compared with WT cells, indicating that cAMP signaling broadly affects protein degradation via the ubiquitin/proteasome pathway.
authors.affiliation: Istituto di Bioscienze e Biorisorse, CNR, Via G. Amendola 165/A, 70126, Bari, Italy.
authors.forename: Wilma
authors.initials: W
authors.lastname: Sabetta
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via H. J. Dunant 3, 21100, Varese, Italy.
authors.forename: Candida
authors.initials: C
authors.lastname: Vannini
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", via E. Orabona 4, 70125, Bari, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sgobba
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via H. J. Dunant 3, 21100, Varese, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Marsoni
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", via E. Orabona 4, 70125, Bari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Paradiso
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via H. J. Dunant 3, 21100, Varese, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ortolani
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via H. J. Dunant 3, 21100, Varese, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Bracale
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", via E. Orabona 4, 70125, Bari, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Viggiano
authors.affiliation: Istituto di Bioscienze e Biorisorse, CNR, Via G. Amendola 165/A, 70126, Bari, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Blanco
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", via E. Orabona 4, 70125, Bari, Italy. mariaconcetta.depinto@uniba.it.
authors.forename: Maria Concetta
authors.initials: MC
authors.lastname: de Pinto
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D030821:Plants, Genetically Modified; D040901:Proteomics; D013312:Stress, Physiological / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology*; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D000242:Cyclic AMP; D013482:Superoxide Dismutase
keywords: Antioxidant systems; Cell cycle; Cell stress; Cyclic AMP; Tobacco BY-2 cells; cAMP-sponge