title: The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein.
abstract: Tetrapyrrole biosynthesis is an essential and tightly regulated process, and glutamyl-tRNA reductase (GluTR) is a key target for multiple regulatory factors at the post-translational level. By binding to the thylakoid membrane protein FLUORESCENT (FLU) or the soluble stromal GluTR-binding protein (GBP), the activity of GluTR is down- or up-regulated. Here, we reconstructed a ternary complex composed of the C-terminal tetratricopepetide-repeat domain of FLU, GBP, and GluTR, crystallized and solved the structure of the complex at 3.2 Å. The overall structure resembles the shape of merged two binary complexes as previously reported, and shows a large conformational change within GluTR. We also demonstrated that GluTR binds tightly with GBP but does not bind to GSAM under the same condition. These findings allow us to suggest a biological role of the ternary complex for the regulation of plant GluTR.
authors.affiliation: Key Laboratory of Photobiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Key Laboratory of Photobiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Feilong
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photobiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|College of Life Sciences, Northwest A&F University, Xi'an, Shaanxi, 712100, China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Photobiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photobiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002151:Calorimetry; D002352:Carrier Proteins / Q000378:metabolism*; D018360:Crystallography, X-Ray; D019751:Intramolecular Transferases / Q000378:metabolism; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C478633:FLU protein, Arabidopsis; D046912:Multiprotein Complexes; C000613314:glutamyl-tRNA reductase-binding protein, Arabidopsis; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase; C000613313:glutamyl-tRNA reductase, Arabidopsis; D019751:Intramolecular Transferases; C061049:glutamate-1-semialdehyde 2,1-aminomutase