title: Development and inter-laboratory transfer of a decaplex polymerase chain reaction assay combined with capillary electrophoresis for the simultaneous detection of ten food allergens.
abstract: Food allergies cause health risks to susceptible consumers and regulations on labeling of food allergen contents have been implemented in many countries and regions. To achieve timely and accurate food allergen labeling, the development of fast and effective allergen detection methods is very important. Herein, a decaplex polymerase chain reaction (PCR) assay combined with capillary electrophoresis was developed to detect simultaneously 10 common food allergens from hazelnut, pistachio, oat, sesame, peanut, cashew, barley, wheat, soybean and pecan. The absolute limit of detection (LODa) of this system is between 2 and 20 copies of haploid genome, and the relative LOD (LODr) is as low as 0.005% (w/w) in simulated food mixtures. The developed assay was subsequently applied to 20 commercial food products and verified the allergen ingredients stated on the labels. Furthermore, results using this decaplex PCR assay was successfully replicated in three other laboratories, demonstrating the repeatability and applicability of this assay in routine analysis of the 10 food allergens.
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-BorLuh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-BorLuh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Agilent Technologies (China) Co., Ltd., 3 Wang Jing Bei Lu, Chao Yang District, Beijing 100102, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-BorLuh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China.
authors.forename: Aihu
authors.initials: A
authors.lastname: Pan
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-BorLuh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Quan
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-BorLuh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China; Agilent Technologies (China) Co., Ltd., 3 Wang Jing Bei Lu, Chao Yang District, Beijing 100102, People's Republic of China.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: HaeYeong
authors.initials: H
authors.lastname: Kim
authors.affiliation: GMO Detection Laboratory, Shanghai Entry-Exit Inspection and Quarantine Bureau, 1208 Minsheng Road, Shanghai 200135, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Agilent Technologies (China) Co., Ltd., 3 Wang Jing Bei Lu, Chao Yang District, Beijing 100102, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-BorLuh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China. Electronic address: yylltt@sjtu.edu.cn.
authors.forename: Litao
authors.initials: L
authors.lastname: Yang
mesh_terms: D000485:Allergens / Q000276:immunology; D005504:Food Analysis / Q000379:methods*; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000485:Allergens
keywords: Capillary electrophoresis; Decaplex polymerase chain reaction; Food allergen; Inter-laboratory transfer