title: Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors.
abstract: Human induced pluripotent stem cells (hiPSCs) hold great promise as a cell source for therapeutic applications and regenerative medicine. Traditionally, hiPSCs are expanded in two-dimensional static culture as colonies in the presence or absence of feeder cells. However, this expansion procedure is associated with lack of reproducibility and low cell yields. To fulfill the large cell number demand for clinical use, robust large-scale production of these cells under defined conditions is needed. Herein, we describe a scalable, low-cost protocol for expanding hiPSCs as aggregates in a lab-scale bioreactor.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, Canada, AB T2N 1N4.
authors.forename: Walaa
authors.initials: W
authors.lastname: Almutawaa
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, Canada, AB T2N 1N4.
authors.forename: Leili
authors.initials: L
authors.lastname: Rohani
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, Canada, AB T2N 1N4. rancourt@ucalgary.ca.
authors.forename: Derrick E
authors.initials: DE
authors.lastname: Rancourt
mesh_terms: D019149:Bioreactors; D002449:Cell Aggregation; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival; D004734:Energy Metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
keywords: Bioreactor; Energy metabolism; Induced pluripotent stem cells; Pluripotency