title: Temperature during the last week of incubation. I. Effects on hatching pattern and broiler chicken embryonic organ development.
abstract: We investigated the effects of an eggshell temperature (EST) of 35.6, 36.7, 37.8, and 38.9°C applied from d of incubation (E) 15, E17, and E19 on hatching pattern and embryonic organ development. A total of 2,850 first-grade eggs of a 43-week-old Ross 308 broiler breeder flock were incubated at an EST of 37.8°C until E15. From E15, E17, or E19 onward, eggs were incubated at an EST of 35.6, 36.7, 37.8, or 38.9°C. Moment of internal pipping (IP), external pipping (EP), and hatch was determined, and organ development was measured at E15, E17, E19, IP, EP, and hatch. A lower EST extended incubation duration compared to a higher EST. The lower incubation duration was mainly caused by the extended time until IP, whereas time between IP and hatch hardly varied between treatments. Relative heart weight was affected by EST already from 2 d after the start of EST treatment on E15, and effects became more pronounced at longer exposure time to various EST treatments. At hatch, the largest difference in relative heart weight was found between an EST of 35.6 and 38.9°C started at E15 (Δ=64.4%). From E17 onward, EST affected yolk-free body mass (YFBM) and relative stomach weight, where a lower EST resulted in a lower YFBM and relative stomach weight before IP and a higher YFBM and relative stomach weight after IP. From E19 onward, a lower EST resulted in a higher relative liver and spleen weight regardless of start time of treatment. Yolk weight and relative intestine weight were not affected by EST before and at E19, but a higher EST resulted in a higher yolk weight and lower relative intestine weight from IP onward. Based on the higher YFBM and higher relative organ growth found at hatch, we concluded that an EST lower than 37.8°C from E15 onward appears to be beneficial for optimal embryo development.
authors.affiliation: HatchTech BV, P.O. Box 256, 3900 AG Veenendaal, the Netherlands Adaptation Physiology Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands conny.maatjens@wur.nl.
authors.forename: C M
authors.initials: CM
authors.lastname: Maatjens
authors.affiliation: HatchTech BV, P.O. Box 256, 3900 AG Veenendaal, the Netherlands.
authors.forename: I A M
authors.initials: IA
authors.lastname: van Roovert-Reijrink
authors.affiliation: Biometris, Wageningen University, P.O. Box 16, 6700 AA Wageningen, the Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Engel
authors.affiliation: HatchTech BV, P.O. Box 256, 3900 AG Veenendaal, the Netherlands Adaptation Physiology Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands.
authors.forename: C W
authors.initials: CW
authors.lastname: van der Pol
authors.affiliation: Adaptation Physiology Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Kemp
authors.affiliation: Adaptation Physiology Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: van den Brand
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000196:embryology / Q000502:physiology*; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D004528:Egg Shell / Q000196:embryology / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000502:physiology*; D009929:Organ Size; D013696:Temperature
keywords: embryonic organ development; incubation; temperature