title: Induction of tolerance against the arthritogenic antigen with type-II collagen peptide-linked soluble MHC class II molecules.
abstract: In murine collagen-induced arthritis (CIA), self-reactive T cells can recognize peptide antigens derived from type-II collagen (CII). Activation of T cells is an important mediator of autoimmune diseases. Thus, T cells have become a focal point of study to treat autoimmune diseases. In this study, we evaluated the efficacy of recombinant MHC class II molecules in the regulation of antigen-specific T cells by using a self peptide derived from CII (CII260-274; IAGFKGEQGPKGEPG) linked to mouse I-A(q) in a murine CIA model. We found that recombinant I-A(q)/CII260-274 molecules could be recognized by CII-specific T cells and inhibit the same T cells in vitro. Furthermore, the development of CIA in mice was successfully prevented by in vivo injection of recombinant I-A(q)/CII260-274 molecules. Thus, treatment with recombinant soluble MHC class II molecules in complex with an immunodominant self-peptide might offer a potential therapeutic for chronic inflammation in autoimmune disease such as rheumatoid arthritis. [BMB Reports 2016; 49(6): 331-336].
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Yoon-Kyung
authors.initials: YK
authors.lastname: Park
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Sundo
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Se-Ho
authors.initials: SH
authors.lastname: Park
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D024043:Collagen Type II / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007108:Immune Tolerance; D018836:Inflammation Mediators / Q000378:metabolism; D018655:Lymphocyte Count; D051379:Mice; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012995:Solubility
chemical_list: D000941:Antigens; D024043:Collagen Type II; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D018836:Inflammation Mediators; D010455:Peptides; D011994:Recombinant Proteins