title: Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic ψχ heterodimer.
abstract: Several X-ray crystal structures of the E. coli core clamp loader containing the five core (δ', δ, and three truncated γ) subunits have been determined, but they lack the ψ and χ subunits. We report the first solution structure of the complete seven-subunit clamp loader complex using small angle X-ray scattering. This structure not only provides information about the location of the χ and ψ subunits but also provides a model of the dynamic nature of the clamp loader complex.
authors.affiliation: Biochemistry and Molecular Biology, University of Florida , P.O. BOX 100245, Gainesville, Florida 32610, USA.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Tondnevis
authors.affiliation: Cornell High Energy Synchrotron Source (CHESS), Cornell University , 161 Synchrotron Drive, Ithaca, New York 14853, USA.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Gillilan
authors.affiliation: Biochemistry and Molecular Biology, University of Florida , P.O. BOX 100245, Gainesville, Florida 32610, USA.
authors.forename: Linda B
authors.initials: LB
authors.lastname: Bloom
authors.affiliation: Biochemistry and Molecular Biology, University of Florida , P.O. BOX 100245, Gainesville, Florida 32610, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: McKenna