title: Phospholipase A2-Induced Remodeling Processes on Liquid-Ordered/Liquid-Disordered Membranes Containing Docosahexaenoic or Oleic Acid: A Comparison Study.
abstract: Vesicle cycling, which is an important biological event, involves the interplay between membrane lipids and proteins, among which the enzyme phospholipase A2 (PLA2) plays a critical role. The capacity of PLA2 to trigger the budding and fission of liquid-ordered (L(o)) domains has been examined in palmitoyl-docosahexaenoylphosphatidylcholine (PDPC) and palmitoyl-oleoylphosphatidylcholine (POPC)/sphingomyelin/cholesterol membranes. They both exhibited a L(o)/liquid-disordered (L(d)) phase separation. We demonstrated that PLA2 was able to trigger budding in PDPC-containing vesicles but not POPC ones. The enzymatic activity, line tension, and elasticity of the membrane surrounding the L(o) domains are critical for budding. The higher line tension of Lo domains in PDPC mixtures was assigned to the greater difference in order parameters of the coexisting phases. The higher amount of lysophosphatidylcholine generated by PLA2 in the PDPC-containing mixtures led to a less-rigid membrane, compared to POPC. The more elastic L(d) membranes in PDPC mixtures exert a lower counteracting force against the L(o) domain bending.
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Rayna
authors.initials: R
authors.lastname: Georgieva
authors.affiliation: Biophysical Chemistry Laboratory, Department of Physical Chemistry, Faculty of Chemistry and Pharmacy, University of Sofia , 1 J. Bourchier Str., 1164 Sofia, Bulgaria.
authors.forename: Kristina
authors.initials: K
authors.lastname: Mircheva
authors.affiliation: Institute of Solid State Physics, Bulgarian Academy of Sciences , 72 Tsarigradsko Chaussee, 1784 Sofia, Bulgaria.
authors.forename: Victoria
authors.initials: V
authors.lastname: Vitkova
authors.affiliation: Biophysical Chemistry Laboratory, Department of Physical Chemistry, Faculty of Chemistry and Pharmacy, University of Sofia , 1 J. Bourchier Str., 1164 Sofia, Bulgaria.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Balashev
authors.affiliation: Biophysical Chemistry Laboratory, Department of Physical Chemistry, Faculty of Chemistry and Pharmacy, University of Sofia , 1 J. Bourchier Str., 1164 Sofia, Bulgaria.
authors.forename: Tzvetanka
authors.initials: T
authors.lastname: Ivanova
authors.affiliation: Sorbonne Universites-UPMC Univ Paris 06, UMR 7203, INSERM ERL 1157, CHU St. Antoine, 27 rue Chaligny, 75012 Paris, France.|Department of Psychiatry, Hôpital Saint-Antoine, AP-HP , Paris, France.
authors.forename: Cedric
authors.initials: C
authors.lastname: Tessier
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Kamen
authors.initials: K
authors.lastname: Koumanov
authors.affiliation: Sorbonne Universites-UPMC Univ Paris 06, UMR 7203, INSERM ERL 1157, CHU St. Antoine, 27 rue Chaligny, 75012 Paris, France.|Department of Psychiatry, Hôpital Saint-Antoine, AP-HP , Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Nuss
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Albena
authors.initials: A
authors.lastname: Momchilova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Galya
authors.initials: G
authors.lastname: Staneva
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000378:metabolism; D055119:Elastic Modulus; D019301:Oleic Acid / Q000737:chemistry* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D054467:Phospholipases A2 / Q000378:metabolism*; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism
chemical_list: D010713:Phosphatidylcholines; D013109:Sphingomyelins; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D002784:Cholesterol; D054467:Phospholipases A2; C028694:1-palmitoyl-2-oleoylphosphatidylcholine