title: Functional and Genetic Analysis of Epiplakin in Epithelial Cells.
abstract: Epiplakin is a large member (>700 kDa) of the plakin protein family and exclusively expressed in epithelial cell types. Compared to other plakin proteins epiplakin exhibits an unusual structure as it consists entirely of a variable number of consecutive plakin repeat domains (13 in humans, 16 in mice). The only binding partners of epiplakin identified so far are keratins of simple as well as of stratified epithelia. Epiplakin-deficient mice show no obvious spontaneous phenotype. However, ex vivo studies using epiplakin-deficient primary cells indicated protective functions of epiplakin in response to stress. Recent studies using stress models for organs of the gastrointestinal tract revealed that epiplakin-deficient mice develop more pronounced pancreas and liver injuries than their wild-type littermates. In addition, impaired stress-induced keratin network reorganization was observed in the affected organs, and primary epiplakin-deficient hepatocytes showed reduced tolerance for forced keratin overexpression which could be rescued by a chemical chaperone. These findings indicate protective functions of epiplakin in chaperoning disease-induced keratin reorganization. In this review, we describe some of the methods we used to analyze epiplakin's function with the focus on biochemical and ex vivo techniques.
authors.affiliation: Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Vienna, Austria.
authors.forename: Sandra
authors.initials: S
authors.lastname: Szabo
authors.affiliation: Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Vienna, Austria.
authors.forename: Karl L
authors.initials: KL
authors.lastname: Wögenstein
authors.affiliation: Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Vienna, Austria. Electronic address: peter.fuchs@univie.ac.at.
authors.forename: Peter
authors.initials: P
authors.lastname: Fuchs
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000502:physiology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000502:physiology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D018345:Mice, Knockout; D061251:Primary Cell Culture
chemical_list: D001324:Autoantigens; C426678:epiplakin; D007633:Keratins
keywords: Chaperone like; Epiplakin; Epithelia; Keratin binding; Keratins; Mouse stress models; Plakin