title: [Difficulties and limitations in conducting translational research in thoracic oncology. A practical example].
abstract: BACKGROUND
  In a first study, we identified signatures of 3 mRNAs (semaphorin 3D [SEMA3D], cytokeratin 16 [KRT16] and UL16 binding protein 2 [ULBP2]) associated to response to a cisplatin-vinorelbin chemotherapy and to survival of advanced non-small cell lung cancers (NSCLC).
  
  
  METHODS
  The aim of this study was to develop immunohistochemistry tests for KRT16, ULBP2 and SEMA3D and to test proteins expression for prediction of response and survival in biopsies of the same patients.
  
  
  RESULTS
  We were not able to reproduce by the protein expression study the signature predicting response to chemotherapy in advanced NSCLC.
  
  
  CONCLUSIONS
  We highlight the difficulties of translational research in thoracic oncology emphasizing the complexity in obtaining adequate tissue samples and the difficulties in conduction and transposing in routine practice high throughput technique for transcriptomic analyses.
authors.affiliation: Service des soins intensifs, urgences oncologiques et oncologie thoracique, institut Jules-Bordet, université libre de Bruxelles, 1, rue Héger-Bordet, 1000 Bruxelles, Belgique. Electronic address: ap.meert@bordet.be.
authors.forename: A-P
authors.initials: AP
authors.lastname: Meert
authors.affiliation: Data centre, institut Jules-Bordet, université libre de Bruxelles, Bruxelles, Belgique.
authors.forename: L
authors.initials: L
authors.lastname: Ameye
authors.affiliation: Service des soins intensifs, urgences oncologiques et oncologie thoracique, institut Jules-Bordet, université libre de Bruxelles, 1, rue Héger-Bordet, 1000 Bruxelles, Belgique.
authors.forename: N
authors.initials: N
authors.lastname: Leclercq
authors.affiliation: Data centre, institut Jules-Bordet, université libre de Bruxelles, Bruxelles, Belgique.
authors.forename: M
authors.initials: M
authors.lastname: Paesmans
authors.affiliation: Service d'anatomopathologie, hôpital Erasme, université libre de Bruxelles, Bruxelles, Belgique.
authors.forename: M
authors.initials: M
authors.lastname: Remmelink
authors.affiliation: Service des soins intensifs, urgences oncologiques et oncologie thoracique, institut Jules-Bordet, université libre de Bruxelles, 1, rue Héger-Bordet, 1000 Bruxelles, Belgique.
authors.forename: J-P
authors.initials: JP
authors.lastname: Sculier
authors.affiliation: Service des soins intensifs, urgences oncologiques et oncologie thoracique, institut Jules-Bordet, université libre de Bruxelles, 1, rue Héger-Bordet, 1000 Bruxelles, Belgique.
authors.forename: T
authors.initials: T
authors.lastname: Berghmans
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000188:drug therapy / Q000378:metabolism / Q000401:mortality; D002945:Cisplatin / Q000008:administration & dosage; D058851:GPI-Linked Proteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D053536:Keratin-16 / Q000032:analysis / Q000378:metabolism*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality / Q000628:therapy; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D011379:Prognosis; D015203:Reproducibility of Results; D039961:Semaphorins / Q000032:analysis / Q000378:metabolism*; D012680:Sensitivity and Specificity; D016019:Survival Analysis; D057170:Translational Research, Biomedical / Q000379:methods / Q000592:standards; D014747:Vinblastine / Q000008:administration & dosage / Q000031:analogs & derivatives; D000077235:Vinorelbine
chemical_list: D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D036341:Intercellular Signaling Peptides and Proteins; C507431:KRT16 protein, human; D053536:Keratin-16; C000592402:Sema3D protein, human; D039961:Semaphorins; C423230:ULBP2 protein, human; D014747:Vinblastine; D002945:Cisplatin; D000077235:Vinorelbine
keywords: Cancer bronchique; Chemotherapy; Chimiothérapie; Facteurs prédictifs; Lung neoplasm; Predictive factors; Response; Réponse; Survie; Survival