title: Washed versus unwashed red blood cells for transfusion for the prevention of morbidity and mortality in preterm infants.
abstract: BACKGROUND
  Infants born very preterm often receive multiple red blood cell (RBC) transfusions during their initial hospitalisation. However, there is an increasing awareness of potential adverse effects of RBC transfusions in this vulnerable patient population. Modification of RBCs prior to transfusion, through washing with 0.9% saline, may reduce these adverse effects and reduce the rate of significant morbidity and mortality for preterm infants and improve outcomes for this high-risk group.
  
  
  OBJECTIVE
  To determine whether pre-transfusion washing of RBCs prevents morbidity and mortality in preterm infants.
  
  
  METHODS
  We used the standard search strategy of the Cochrane Neonatal Review Group to search the Cochrane Central Register of Controlled Trials (CENTRAL 2015, Issue 7), MEDLINE via PubMed (31 July 2015), EMBASE (31 July 2015), and CINAHL (31 July 2015). We also searched clinical trials databases, conference proceedings, and the reference lists of retrieved articles for randomised controlled trials and quasi-randomised trials.
  
  
  METHODS
  Randomised, cluster randomised, and quasi-randomised controlled trials including preterm infants (less than 32 weeks gestation) or very low birth weight infants (less than 1500 g), or both, who received one or more washed packed RBC transfusions.
  
  
  METHODS
  Two review authors independently assessed the eligibility of the trials. We identified four studies from the initial search. After further review of the full-text studies, we found one study meeting the selection criteria.
  
  
  RESULTS
  We included a single study enrolling a total of 21 infants for analysis in this review and reported on all-cause mortality during hospital stay, length of initial neonatal intensive care unit (NICU) stay (days), and duration of mechanical ventilation (days). There was no significant difference in mortality between the washed versus the unwashed RBCs for transfusion groups (risk ratio 1.63, 95% confidence interval (CI) 0.28 to 9.36; risk difference 0.10, 95% CI -0.26 to 0.45). There was no significant difference in the length of initial NICU stay between the washed versus the unwashed RBCs for transfusion groups (mean difference (MD) 25 days, 95% CI -21.15 to 71.15) or the duration of mechanical ventilation between the washed versus the unwashed RBCs for transfusion groups (MD 9.60 days, 95% CI -1.90 to 21.10).
  
  
  CONCLUSIONS
  We identified a single small study. The results from this study show a high level of uncertainty, as the confidence intervals are consistent with both a large improvement or a serious harm caused by the intervention. Consequently, there is insufficient evidence to support or refute the use of washed RBCs to prevent the development of significant neonatal morbidities or mortality. Further clinical trials are required to assess the potential effects of pre-transfusion washing of RBCs for preterm or very low birth weight infants, or both, on short- and long-term outcomes.
authors.affiliation: Robinson Research Institute, University of Adelaide, 72 King William Road, Adelaide, Australia.
authors.forename: Amy K
authors.initials: AK
authors.lastname: Keir
authors.forename: Dominic
authors.initials: D
authors.lastname: Wilkinson
authors.forename: Chad
authors.initials: C
authors.lastname: Andersen
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Stark
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D017707:Erythrocyte Transfusion / Q000009:adverse effects / Q000379:methods / Q000401:mortality; D004912:Erythrocytes; D006801:Humans; D062071:Infant, Extremely Premature; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D007363:Intensive Care Units, Neonatal / Q000706:statistics & numerical data; D007902:Length of Stay / Q000706:statistics & numerical data; D016032:Randomized Controlled Trials as Topic; D012121:Respiration, Artificial / Q000706:statistics & numerical data; D012965:Sodium Chloride; D013997:Time Factors
chemical_list: D012965:Sodium Chloride