title: Comparisons of the transferability of plasmids pCAR1, pB10, R388, and NAH7 among Pseudomonas putida at different cell densities.
abstract: The transferability of plasmids pCAR1, pB10, R388, and NAH7 was compared using the same donor-recipient system at different cell density combinations in liquid or on a solid surface. pCAR1 was efficiently transferred in liquid, whereas the other plasmids were preferentially transferred on a solid surface. Difference of liquid or solid affected the transfer frequency especially at lower cell densities.
authors.affiliation: a Biotechnology Research Center , The University of Tokyo , Tokyo , Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yanagida
authors.affiliation: a Biotechnology Research Center , The University of Tokyo , Tokyo , Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Sakuda
authors.affiliation: a Biotechnology Research Center , The University of Tokyo , Tokyo , Japan.
authors.forename: Chiho
authors.initials: C
authors.lastname: Suzuki-Minakuchi
authors.affiliation: b Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology , Shizuoka University , Hamamatsu , Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Shintani
authors.affiliation: a Biotechnology Research Center , The University of Tokyo , Tokyo , Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Matsui
authors.affiliation: a Biotechnology Research Center , The University of Tokyo , Tokyo , Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.affiliation: a Biotechnology Research Center , The University of Tokyo , Tokyo , Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D058491:Bacterial Load; D003227:Conjugation, Genetic; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D022761:Gene Transfer, Horizontal; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial
keywords: Pseudomonas putida; cell density; conjugation; horizontal gene transfer; plasmid