title: Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes.
abstract: Aged testes undergo profound histological and morphological alterations leading to a reduced functionality. Here, we investigated whether variations in longevity affect the development of local inflammatory processes, the oxidative state and the occurrence of apoptotic events in the testis. To this aim, well-established mouse models with delayed (growth hormone releasing hormone-knockout and Ames dwarf mice) or accelerated (growth hormone-transgenic mice) aging were used. We hereby show that the testes of short-lived mice show a significant increase in cyclooxygenase 2 expression, PGD2 production, lipid peroxidation, antioxidant enzymes expression, local macrophages and TUNEL-positive germ cells numbers, and the levels of both pro-caspase-3 and cleaved caspase-3. In contrast, although the expression of antioxidant enzymes remained unchanged in testes of long-lived mice, the remainder of the parameters assessed showed a significant reduction. This study provides novel evidence that longevity confers anti-inflammatory, anti-oxidant and anti-apoptotic capacities to the adult testis. Oppositely, short-lived mice suffer testicular inflammatory, oxidative and apoptotic processes.
authors.affiliation: Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2 Ciudad de Buenos Aires, Argentina.|Cátedra de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Matzkin
authors.affiliation: Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.
authors.forename: Johanna Gabriela
authors.initials: JG
authors.lastname: Miquet
authors.affiliation: Geriatrics Research, Department of Internal Medicine, School of Medicine, Southern Illinois University, Springfield, IL 62794, USA.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Geriatrics Research, Department of Internal Medicine, School of Medicine, Southern Illinois University, Springfield, IL 62794, USA.|Department of Medical Microbiology, Immunology and Cell Biology, School of Medicine, Southern Illinois University, Springfield, IL 62794, USA.
authors.forename: Cristal Monique
authors.initials: CM
authors.lastname: Hill
authors.affiliation: Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.
authors.forename: Daniel
authors.initials: D
authors.lastname: Turyn
authors.affiliation: Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2 Ciudad de Buenos Aires, Argentina.
authors.forename: Ricardo Saúl
authors.initials: RS
authors.lastname: Calandra
authors.affiliation: Geriatrics Research, Department of Internal Medicine, School of Medicine, Southern Illinois University, Springfield, IL 62794, USA.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bartke
authors.affiliation: Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2 Ciudad de Buenos Aires, Argentina.|Cátedra de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.
authors.forename: Mónica Beatriz
authors.initials: MB
authors.lastname: Frungieri
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000172:deficiency / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism*; D007985:Leydig Cells / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018384:Oxidative Stress; D010641:Phenotype; D015230:Prostaglandin D2 / Q000378:metabolism; D015398:Signal Transduction; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; D018836:Inflammation Mediators; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; C505409:Casp3 protein, mouse; D053148:Caspase 3; D015230:Prostaglandin D2
keywords: aging; apoptosis; inflammation; oxidative stress; testis