title: Role of Proteolipid Protein in HSV-1 Entry in Oligodendrocytic Cells.
abstract: Herpes simplex virus type 1 (HSV-1) has the ability to enter many different hosts and cell types by several strategies. This highly prevalent alphaherpesvirus can enter target cells using different receptors and different pathways: fusion at a neutral pH, low-pH-dependent and low-pH-independent endocytosis. Several cell receptors for viral entry have been described, but several observations suggest that more receptors for HSV-1 might exist. In this work, we propose a novel role for the proteolipid protein (PLP) in HSV-1 entry into the human oligodendrocytic cell line HOG. Cells transfected with PLP-EGFP showed an increase in susceptibility to HSV-1. Furthermore, the infection of HOG and HOG-PLP transfected cells with the R120vGF virus--unable to replicate in ICP4-defficient cells--showed an increase in viral signal in HOG-PLP, suggesting a PLP involvement in viral entry. In addition, a mouse monoclonal antibody against PLP drastically inhibited HSV-1 entry into HOG cells. PLP and virions colocalized in confocal immunofluorescence images, and in electron microscopy images, which suggest that PLP acts at the site of entry into HOG cells. Taken together these results suggest that PLP may be involved in HSV-1 entry in human oligodendrocytic cells.
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049, Madrid, Spain.|Universidad Complutense de Madrid, Facultad de Medicina, Plaza de Ramón y Cajal, s/n Ciudad Universitaria, 28040, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Bello-Morales
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Antonio Jesús
authors.initials: AJ
authors.lastname: Crespillo
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Praena
authors.affiliation: Universidad Autónoma de Madrid, Facultad de Medicina, Arzobispo Morcillo 4, 28029, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Tabarés
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 5, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Revilla
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 5, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: García
authors.affiliation: Universidad Complutense de Madrid, Facultad de Medicina, Plaza de Ramón y Cajal, s/n Ciudad Universitaria, 28040, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fraile-Ramos
authors.affiliation: University of Groningen, Faculty of Medical Sciences, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
authors.forename: Wia
authors.initials: W
authors.lastname: Baron
authors.affiliation: Department of Biological Sciences and Department of Biomedical and Translational Sciences, Rowan University, Glassboro, NJ, 08028, United States of America.
authors.forename: Claude
authors.initials: C
authors.lastname: Krummenacher
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049, Madrid, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: López-Guerrero
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006801:Humans; D018991:Myelin Proteolipid Protein / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism* / Q000821:virology; D053586:Virus Internalization
chemical_list: D018991:Myelin Proteolipid Protein; C472930:PLP1 protein, human