title: Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC-ESIMS/MS identification of phenolic components.
abstract: Cucurbitaceae are one of most widely used plant species for human food but lesser known members have not been examined for bioactive components. The purpose of this study was to evaluate the antioxidant and genoprotective activities from three cucurbitaceae seeds extracts and to identify phenolic components by LC-ESIMS/MS analysis. From the results, the yield of seeds extract was 20-41% (w/w) and samples had 16-40% total phenols as gallic acid equivalents (GAE). Compared with methanol solvent, using acidified methanol led to increased extraction yield by 1.4 to 10-fold, higher phenolic content (149.5 ± 1.2 to 396.4 ± 1.9 mg GAE/g), higher DPPH radical quenching and enhanced genoprotective activity using the pBR322 plasmid assay. LC-ESI-MS/MS analysis led to identification of 14-17 components, based on authentic standards and comparison with literature reports, as mainly phenolic acids and esters, flavonol glycosides. This may be the first mass spectrometric profiling of polyphenol components from cucurbitaceae seeds.
authors.affiliation: Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yasir
authors.affiliation: Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan; Department of Chemistry, University of California, CA 95616, USA.
authors.forename: Bushra
authors.initials: B
authors.lastname: Sultana
authors.affiliation: School of Biomedical Sciences, Ulster University, Coleraine BT52 ISA, United Kingdom.
authors.forename: Poonam Singh
authors.initials: PS
authors.lastname: Nigam
authors.affiliation: School of Biomedical Sciences, Ulster University, Coleraine BT52 ISA, United Kingdom. Electronic address: r.owusu-apenten@ulster.ac.uk.
authors.forename: Richard
authors.initials: R
authors.lastname: Owusu-Apenten
mesh_terms: D016587:Antimutagenic Agents / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D002853:Chromatography, Liquid; D019662:Cucurbitaceae / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D044948:Flavonols / Q000032:analysis; D006801:Humans; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D059808:Polyphenols / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D044948:Flavonols; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols
keywords: 4-O-Feruloylquinic acid (PubChem CID: 6171347); Antioxidant; Caftaric acid (PubChem CID: 6440397); Chicoric acid (PubChem CID: 5281764); Cucurbitaceae seeds; Genoprotective; Isoquercetin quercetin 3-O-glucoside (PubChem CID: 5280804); Kaemferol (PubChem CID: 5280863); LC–ESI-MS/MS analysis; Myricetin (PubChem CID: 5281672); Phenolic glycosides; Quercitrin (Quercetin 3-O-rhamnoside) PubChem CID: 535943; Rosmarinic acid (PubChem CID: 5281792); Synapic acid (PubChem CID: 10743); Syringic acid (PubChem CID: 10742); Total phenols; pBR322 plasmid