title: Hexabromocyclododecane (HBCD) induced changes in the liver proteome of eu- and hypothyroid female rats.
abstract: Hexabromocyclododecane (HBCD) is a brominated flame retardant known for its low acute toxicity as observed in animal experiments. However, HBCD exposure can affect liver functioning and thyroid hormone (TH) status. As exact mechanisms are unknown and only limited toxicological data exists, a gel-based proteomic approach was undertaken. In a eu- and hypothyroid female rat model, rats were exposed to 3 and 30 mg/kg bw/day HBCD for 7 days via their diet, and exposure was related to a range of canonical endpoints (hormone status, body weight) available for these animals. Alterations in the liver proteome under HBCD exposure were determined in comparison with patterns of control animals, for both thyroid states. This revealed significantly changed abundance of proteins involved in metabolic processes (gluconeogenesis/glycolysis, amino acid metabolism, lipid metabolism), but also in oxidative stress responses, in both euthyroid and hypothyroid rats. The results provide a more detailed picture on the mechanisms involved in these alterations, e.g. at the protein level changes of the proposed influence of HBCD on the lipid metabolism. Present results show that proteomic approaches can provide further mechanistic insights in toxicological studies.
authors.affiliation: Institute for Medical Biochemistry, Department for Biomedical Sciences, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria. Electronic address: ingrid.miller@vetmeduni.ac.at.
authors.forename: I
authors.initials: I
authors.lastname: Miller
authors.affiliation: Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5, Avenue des Hauts-Forneaux, L-4362 Esch-sur-Alzette, Luxembourg. Electronic address: tommaso.serchi@list.lu.
authors.forename: T
authors.initials: T
authors.lastname: Serchi
authors.affiliation: Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5, Avenue des Hauts-Forneaux, L-4362 Esch-sur-Alzette, Luxembourg. Electronic address: sebastien.cambier@list.lu.
authors.forename: S
authors.initials: S
authors.lastname: Cambier
authors.affiliation: Wageningen University, Human and Animal Physiology Group, P.O. Box 338, 6700 AH Wageningen, The Netherlands. Electronic address: charlenediepenbroek@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Diepenbroek
authors.affiliation: Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5, Avenue des Hauts-Forneaux, L-4362 Esch-sur-Alzette, Luxembourg. Electronic address: jenny.renaut@list.lu.
authors.forename: J
authors.initials: J
authors.lastname: Renaut
authors.affiliation: Wageningen University, Division of Toxicology, Tuinlaan 5, 6703 HE Wageningen, The Netherlands. Electronic address: Hans.vandenBerg@wur.nl.
authors.forename: J H J
authors.initials: JH
authors.lastname: Van der Berg
authors.affiliation: Wageningen Institute for Marine Resources & Ecosystem Studies, IMARES, IJmuiden, The Netherlands. Electronic address: christiaan.kwadijk@wur.nl.
authors.forename: C
authors.initials: C
authors.lastname: Kwadijk
authors.affiliation: Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5, Avenue des Hauts-Forneaux, L-4362 Esch-sur-Alzette, Luxembourg. Electronic address: arno.gutleb@list.lu.
authors.forename: A C
authors.initials: AC
authors.lastname: Gutleb
authors.affiliation: Wageningen University, Human and Animal Physiology Group, P.O. Box 338, 6700 AH Wageningen, The Netherlands; Charité-Universitätsmedizin Berlin, Institute for Experimental Endocrinology, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: eddy.rijntjes@charite.de.
authors.forename: E
authors.initials: E
authors.lastname: Rijntjes
authors.affiliation: Wageningen University, Division of Toxicology, Tuinlaan 5, 6703 HE Wageningen, The Netherlands. Electronic address: Tinka.Murk@WUR.nl.
authors.forename: A J
authors.initials: AJ
authors.lastname: Murk
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D005411:Flame Retardants / Q000633:toxicity*; D006728:Hormones / Q000097:blood; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D007037:Hypothyroidism / Q000473:pathology*; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D020543:Proteome / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000187:drug effects*; D013963:Thyroid Hormones / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D005411:Flame Retardants; D006728:Hormones; D006842:Hydrocarbons, Brominated; D020543:Proteome; D013963:Thyroid Hormones; C089796:hexabromocyclododecane
keywords: HBCD; Hypothyroidism; Lipid metabolism; Liver; Proteomics; Rat