title: Comparative analysis of the morphology and histochemistry of the duodenum of the coypu (Myocastor coypus bonariensis) during its prenatal and postnatal development.
abstract: The objective of this study focused on the comparative morphological and histochemical analysis of the duodenum of fetuses, juveniles and adult coypu (Myocastor coypus bonariensis), the major socioeconomic wildlife resource of Argentina. Histological and histochemical procedures for in situ characterization of glycoconjugates (GCs) were used. This study evidenced that fetal mucins differ histochemically in many respects from their adult counterparts. Only in fetuses from 90 days-post coitus (dpc) glycogen-rich sites were observed throughout the duodenal epithelium. The goblet cells appeared from 105 dpc and their secretory content varied considerably after birth. Duodenal glands presented scanty neutral and sulphated GCs in the 30-day juveniles; in adults the proportion of these GCs increased, and carboxylated and sialylated GCs were also observed. The results obtained in this work may be used in future studies to evaluate the effects of diet and intestinal pathologies in the glycosylation pattern of GCs. Also, knowledge of the normal glycoprofile of the duodenum of M. coypus bonariensis during its ontogenetic development may constitute a basis for the study of this organ in other Hystricognathi rodents.
authors.affiliation: Nacional Council of Scientific and Technical Research (CONICET), Argentina; Institute of Marine and Coastal Research, Nacional Council of Scientific and Technical Research - Nacional University of Mar del Plata, Funes 3250, Mar del Plata 7600, Buenos Aires, Argentina.
authors.forename: María Florencia
authors.initials: MF
authors.lastname: Tano de la Hoz
authors.affiliation: Department of Biological Sciences, Faculty of Veterinary Science, U.N.C.P.B.A., Tandil, Buenos Aires, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Eyheramendy
authors.affiliation: Department of Biological Sciences, Faculty of Veterinary Science, U.N.C.P.B.A., Tandil, Buenos Aires, Argentina.
authors.forename: Antonio Eduardo
authors.initials: AE
authors.lastname: Felipe
authors.affiliation: Institute of Marine and Coastal Research, Nacional Council of Scientific and Technical Research - Nacional University of Mar del Plata, Funes 3250, Mar del Plata 7600, Buenos Aires, Argentina. Electronic address: adiaz@mdp.edu.ar.
authors.forename: Alcira Ofelia
authors.initials: AO
authors.lastname: Díaz
mesh_terms: D000818:Animals; D004386:Duodenum / Q000033:anatomy & histology* / Q000254:growth & development; D004622:Embryo, Mammalian; D047108:Embryonic Development; D005333:Fetus; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000033:anatomy & histology* / Q000254:growth & development; D012377:Rodentia / Q000033:anatomy & histology* / Q000254:growth & development
keywords: Comparative morphology; Duodenum development; Glycoconjugates; Histochemistry; Hystricognathi; Myocastor coypus bonariensis