title: Eliciting cytotoxic T lymphocytes against human laryngeal cancer-derived antigens: evaluation of dendritic cells pulsed with a heat-treated tumor lysate and other antigen-loading strategies for dendritic-cell-based vaccination.
abstract: BACKGROUND
  Dendritic cells (DCs) have been used successfully in clinical pilot studies. However, tumor-specific immunity and clinical responses were only induced in certain cancer patients. It has been well documented that immunotherapy efficacy can be optimized for responses using antigen pulsing.
  
  
  METHODS
  The human laryngeal squamous cell cancer (LSCC) cell line SNU899 was used to evaluate the in vitro anti-tumor efficacy of three different preparations of dendritic cell (DC) vaccines consisting of either whole tumor cells or their derivatives including: i) DCs pulsed with a tumor cell supernatant (DC-TCS), ii) DCs pulsed with whole-cell tumor stressed lysate (DC-TSL), and iii) DCs pulsed with irradiated tumor cells (DC-ITC).
  
  
  RESULTS
  Our results showed that DC-TSL is an effective source of tumor-associated antigens (TAAs) for pulsing DCs. DC-TSL induced the highest expansion of TAA-specific T cells, the strongest Th1 cytokine response, and the most potent cytotoxic T lymphocyte (CTL) activity. DC-TCS and DC-ITC inhibited T cell activation but induced a certain extent of CTL activity.
  
  
  CONCLUSIONS
  These data suggest that DC-TSL is a more potent inducer of antitumor immunity against laryngeal cancer than other antigen-loading strategies using whole tumor cell materials. This strategy provides an alternative approach for DC-based immunotherapy for laryngeal cancer.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. 327826543@qq.com.|Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. 327826543@qq.com.|Department of Otorhinolaryngology Head and Neck Surgery, the Sixth Affiliated Hospital of Sun Yat-Sen University, Yuancun Second Cross Road 26#, Guangzhou, 510655, Guangdong, P.R. China. 327826543@qq.com.
authors.forename: Fan-Qin
authors.initials: FQ
authors.lastname: Wei
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. sunw2@mail2.sysu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Surgery, The University of Hong Kong, Pokfulam Road 102#, Hong Kong, P.R. China. thiansze@gmail.com.
authors.forename: Thian-Sze
authors.initials: TS
authors.lastname: Wong
authors.affiliation: Department of Surgery, The University of Hong Kong, Pokfulam Road 102#, Hong Kong, P.R. China. gaoweiwayne@gmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. yihui-wen@hotmail.com.|Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. yihui-wen@hotmail.com.
authors.forename: Yi-Hui
authors.initials: YH
authors.lastname: Wen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weijiaw@mail2.sysu.edu.cn.|Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weijiaw@mail2.sysu.edu.cn.
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Wei
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weiyi-009@163.com.|Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weiyi-009@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. wenwp@mail.sysu.edu.cn.|Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. wenwp@mail.sysu.edu.cn.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Wen
mesh_terms: D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000628:therapy; D018929:Cell Culture Techniques; D002457:Cell Extracts / Q000276:immunology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007822:Laryngeal Neoplasms / Q000276:immunology* / Q000628:therapy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D002457:Cell Extracts