title: Expression and functional implications of USP17 in glioma.
abstract: Glioma is the most common and malignant brain tumor with extremely poor prognosis. It is crucial to understand the molecular characteristics of glioma and find out more effective therapeutic targets for the treatment of glioma. USP17 is a novel deubiquitinating enzyme that is differentially expressed in certain types of solid tumor. Our present study investigated the pathological functions and clinical significance of USP17 in glioma for the first time. We found that USP17 was down-regulated in glioma tissue compared with normal tissues. Overexpression of USP17 in glioma cells reduced their tumorigenesis and proliferation ability through reducing Ras and Myc protein levels. Subsequent in vivo experiments showed that overexpression of USP17 suppressed tumor progression in an orthotopic glioma models. Further, study of a cohort of 104 patients with stage I-IV glioma showed that USP17 expression was negatively associated with the WHO grade (p<0.001). USP17 was more highly expressed in low grade (I+II) glioma than high-grade (III+IV) glioma (p<0.001). Taken together, our results indicate that USP17 might play important functions in glioma through suppressing glioma tumorigenesis and proliferation.
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, China.
authors.forename: Manmiao
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Huairui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lan
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, China. Electronic address: loumq68128@126.com.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Lou
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D049109:Cell Proliferation; D015536:Down-Regulation; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008297:Male; D008819:Mice, Nude; D008875:Middle Aged; D060787:Neoplasm Grading; D009368:Neoplasm Transplantation
chemical_list: D010450:Endopeptidases; C484854:USP17L2 protein, human
keywords: Glioma; Proliferation; Stage; Tumorigenesis; USP17