title: Transient concentrations of NaCl affect the PHA accumulation in mixed microbial culture.
abstract: The present study explores the feasibility of the accumulation of polyhydroxyalkanoates (PHAs) under the presence of transient concentrations of added sodium chloride, by means of a mixed microbial culture (MMC). This culture was enriched on a mixture of volatile fatty acids (VFAs) containing 0.8g Na(+)/L as NaOH. This MMC presented a maximum PHA accumulation capacity of 53wt% with 27Cmol% HV. Accumulation experiments performed with added NaCl at concentrations of 7, 13 and 20g/L shown that this salt provoked a decrease of the biomass PHA production rate, with an IC50 value close to 7gNaCl/L. The accumulated PHA was lower than the corresponding value of the assay without the addition of salt. Furthermore, the composition of the biopolymer, in terms of HB:HV ratio, changed from 2.71 to 6.37Cmol/Cmol, which means a HV decrease between 27 and 14Cmol%. Summarizing, the PHA accumulation by a MMC non-adapted to saline conditions affected the polymer composition and lead to lower production yields and rates than in absence of added NaCl.
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Rúa Lope Gómez de Marzoa, s/n., E-15705 Santiago de Compostela, Spain. Electronic address: tania.palmeiro@usc.es.
authors.forename: T
authors.initials: T
authors.lastname: Palmeiro-Sánchez
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Rúa Lope Gómez de Marzoa, s/n., E-15705 Santiago de Compostela, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fra-Vázquez
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Rúa Lope Gómez de Marzoa, s/n., E-15705 Santiago de Compostela, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Rey-Martínez
authors.affiliation: Faculty of Engineering and Science, University Adolfo Ibáñez, Avda. Padre Hurtado 750, Viña del Mar, Chile.
authors.forename: J L
authors.initials: JL
authors.lastname: Campos
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Rúa Lope Gómez de Marzoa, s/n., E-15705 Santiago de Compostela, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Mosquera-Corral
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D012965:Sodium Chloride
keywords: Inhibition; Microbial mixed culture; Polyhydroxyalkanoates; Sodium chloride; Volatile fatty acids