title: Application of marrow mesenchymal stem cell-derived extracellular matrix in peripheral nerve tissue engineering.
abstract: To advance molecular and cellular therapy into the clinic for peripheral nerve injury, modification of neural scaffolds with the extracellular matrix (ECM) of peripheral nerves has been established as a promising alternative to direct inclusion of support cells and/or growth factors within a neural scaffold, while cell-derived ECM proves to be superior to tissue-derived ECM in the modification of neural scaffolds. Based on the fact that bone marrow mesenchymal stem cells (BMSCs), just like Schwann cells, are adopted as support cells within a neural scaffold, in this study we used BMSCs as parent cells to generate ECM for application in peripheral nerve tissue engineering. A chitosan nerve guidance conduit (NGC) and silk fibroin filamentous fillers were respectively prepared for co-culture with purified BMSCs, followed by decellularization to stimulate ECM deposition. The ECM-modified NGC and lumen fillers were then assembled into a chitosan-silk fibroin-based, BMSC-derived, ECM-modified neural scaffold, which was implanted into rats to bridge a 10 mm-long sciatic nerve gap. Histological and functional assessments after implantation showed that regenerative outcomes achieved by our engineered neural scaffold were better than those achieved by a plain chitosan-silk fibroin scaffold, and suggested the benefits of BMSC-derived ECM for peripheral nerve repair. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Centre of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Centre of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, People's Republic of China.
authors.forename: Zhenmeiyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hand Surgery, Affiliated Hospital of Nantong University, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Centre of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, People's Republic of China.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Centre of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, People's Republic of China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Hand Surgery, Affiliated Hospital of Nantong University, People's Republic of China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D048271:Chitosan / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D010525:Peripheral Nerves / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005349:Fibroins; D048271:Chitosan
keywords: bone marrow mesenchymal stem cells (BMSCs); chitosan; extracellular matrix (ECM); neural scaffolds; peripheral nerve repair; silk fibroin