title: Functional and Genetic Analysis of VAB-10 Spectraplakin in Caenorhabditis elegans.
abstract: Intermediate filaments (IFs) are involved in multiple cellular processes that are essential for the maintenance of cell and tissue integrity. To achieve this crucial function, IFs have to be organized as long and resistant filaments across the cells and to be tightly anchored at the cell periphery. This anchoring takes place at the level desmosomes and hemidesmosomes through proteins belonging to the spectraplakin family. Here, we focus on the sole nematode Caenorhabditis elegans spectraplakin locus vab-10 that is essential to connect the epidermis to the cuticle apically and to the muscles basally. After briefly reviewing the structure of the gene, we first present the genetic tools available to study this gene as well as the reagents to examine the distribution of its translation products. We discuss the functional assays that enable examining their function. Finally, we detail a genetic method to identify spectraplakin functional partners through RNAi screens, and a biochemical method to examine the phosphorylation status of IFs.
authors.affiliation: IGBMC, Development and Stem Cells Program, CNRS (UMR 7104)/INSERM (U964)/Université de Strasbourg, Illkirch, France. Electronic address: gally@igbmc.fr.
authors.forename: Christelle
authors.initials: C
authors.lastname: Gally
authors.affiliation: Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: IBPS, Paris, France. Electronic address: michel.labouesse@upmc.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Labouesse
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005091:Exons; D009154:Mutation; D017434:Protein Structure, Tertiary
chemical_list: D029742:Caenorhabditis elegans Proteins; C475260:VAB-10 protein, C elegans
keywords: C. elegans hemidesmosomes; Intermediate filaments; Spectraplakin; VAB-10