title: Comparison of immunohistochemistry and mRNAin situhybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue.
abstract: Thyroid transcription factor-1 (TTF-1) reportedly possesses oncogenic and suppressive roles within the same tumor type and may play a dual function in the progression of lung cancer. Immunohistochemistry (IHC) and mRNA in situ hybridization (ISH) are commonly used methods for detecting protein or mRNA expression. The present study compared the concordance rate of the two methods in the evaluation of thyroid transcription factor-1 (TTF-1) expression in non-small cell lung carcinoma (NSCLC) using tissue microarray-based IHC and mRNA ISH. TTF-1 protein and mRNA expression levels were examined in 196 cases of NSCLC. The IHC and mRNA ISH agreement was 91.3% (179/196), and near-perfect agreement was observed between the two methods (κ-coefficient, 0.848). There was no significant difference between IHC and mRNA ISH, as analyzed by the McNemar-Bowker test (P=0.219). The present findings proved that IHC is comparable to mRNA ISH for evaluating TTF-1 expression in NSCLC. These two methods can be used to detect TTF-1 expression in future studies.
authors.affiliation: Department of General Surgery, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110840, P.R. China.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Division of Nephrology, Nanfang Hospital, Southern Medical University and Guangdong Provincial Institute of Nephrology, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Gastroenterology, 202nd Hospital of the People's Liberation Army, Shenyang, Liaoning 110003, P.R. China.
authors.forename: Fanjun
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of General Surgery, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110840, P.R. China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zhang
keywords: immunohistochemistry; mRNA in situ hybridization; non-small cell lung cancer; thyroid transcription factor-1; tissue microarray