title: Agaricus bisporus and its in vitro culture as a source of indole compounds released into artificial digestive juices.
abstract: The popularity of Agaricus bisporus results not only from the quality of the flavors, but also from the content of many substances of therapeutic properties. This paper presents a study on RP-HPLC determination of the content of indole compounds released from the lyophilized biomass from in vitro cultures of A. bisporus into artificial digestive juices at 37°C. A. bisporus in vitro cultures were grown on media enriched with zinc salts. The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the greatest number of samples. The content of 5-hydroxy-l-tryptophan in the investigated samples ranged from 86.62 to 531 mg/100g d.w. The amount of l-tryptophan was determined within the range of 1.63-4.68 mg/100g d.w. and for melatonin 0.43-0.64 mg/100g d.w. It was demonstrated for the first time that in vitro cultures of A. bisporus release indole compounds in conditions simulating the human digestive tract.
authors.affiliation: Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland. Electronic address: muchon@poczta.fm.
authors.forename: Bożena
authors.initials: B
authors.lastname: Muszyńska
authors.affiliation: Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kała
authors.affiliation: Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sułkowska-Ziaja
authors.affiliation: Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków 30-059, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Krakowska
authors.affiliation: Department of Inorganic and Analytical Chemistry, Jagiellonian University, Collegium Medicum, Medyczna St 9, 30-688 Kraków, Poland.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Opoka
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004063:Digestion; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D007419:Intestinal Secretions / Q000378:metabolism*
chemical_list: D007211:Indoles
keywords: Agaricus bisporus; Artificial gastric juices; In vitro culture; Indole compounds; Zinc