title: Implications of anticoagulants and gender on cell counts and growth factor concentration in platelet-rich plasma and platelet-rich gel supernatants from rabbits.
abstract: OBJECTIVE
  Our objectives were as follows: 1) to validate a protocol for producing rabbit platelet-rich plasma (PRP); 2) to determine the influence of two anticoagulants, sodium citrate and acid-citrate-dextrose solution A, and gender on cell count in PRP and growth factor concentration in pure platelet-rich gel supernatants; 3) to correlate the variables evaluated.
  
  
  METHODS
  Whole blood from 18 New Zealand rabbits (9 males and 9 females) was obtained with sodium citrate and acid-citrate-dextrose solution A for processing PRP fractions (A and B), which were evaluated for haematology. The PRP fractions were either activated with calcium gluconate or lysated with a detergent. The concentrations of transforming growth factor beta 1 and platelet-derived growth factor BB were assayed by ELISA.
  
  
  RESULTS
  The sodium citrate PRP-B had significantly higher counts of platelets in comparison to PRP-A and whole blood obtained with the same anticoagulant and the homologous acid-citrate-dextrose solution A PRP fraction. The sodium citrate PRP-A had a significantly higher count of leukocytes compared to the homologous acid-citrate-dextrose solution A fraction. All the PRP fractions had a significant leuko-reduction when compared to whole blood. The sodium citrate PRP-A fraction from female rabbits had significantly lower platelet counts and significantly higher leukocyte counts than the same acid-citrate-dextrose solution A fraction. Growth factor concentration was not affected by the type of anticoagulant or gender.
  
  
  CONCLUSIONS
  The type of anticoagulant and gender affected the cell counts in PRP, but they did not influence the growth factor concentration. More complete rabbit PRP studies should be performed before evaluating this type of substance in models of disease.
authors.forename: Juan C
authors.initials: JC
authors.lastname: González
authors.forename: Catalina
authors.initials: C
authors.lastname: López
authors.affiliation: Prof. Jorge U. Carmona, MVZ, MSc, PhD, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales, Colombia, Phone/Fax: +57 68 781516, E-mail: carmona@ucaldas.edu.co.
authors.forename: Jorge U
authors.initials: JU
authors.lastname: Carmona
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D000077214:Becaplermin; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D007958:Leukocyte Count; D008297:Male; D010956:Plasmapheresis / Q000379:methods / Q000662:veterinary; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000187:drug effects*; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D011817:Rabbits; D012727:Sex Characteristics; D000077559:Sodium Citrate; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D000925:Anticoagulants; D002951:Citrates; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; C002113:acid citrate dextrose; D000077214:Becaplermin; D000077559:Sodium Citrate; D019343:Citric Acid; D005947:Glucose
keywords: Animal model; platelet concentrates; platelet-derived growth factor BB; platelet-rich plasma; rabbit; transforming growth factor beta 1