title: Wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces via entrapment in low potential phenothiazine-modified redox polymers.
abstract: Phenothiazine-modified redox hydrogels were synthesized and used for the wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces. The effects of the pH value and electrode surface modification on the biocatalytic activity of the layers were studied in the presence of vanillin as the substrate. The enzyme electrodes were successfully employed as bioanodes in vanillin/O2 biofuel cells in combination with a high potential bilirubin oxidase biocathode. Open circuit voltages of around 700 mV could be obtained in a two compartment biofuel cell setup. Moreover, the use of a rather hydrophobic polymer with a high degree of crosslinking sites ensures the formation of stable polymer/enzyme films which were successfully used as bioanode in membrane-less biofuel cells.
authors.affiliation: Analytical Chemistry, Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Piyanut
authors.initials: P
authors.lastname: Pinyou
authors.affiliation: Analytical Chemistry, Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Adrian
authors.initials: A
authors.lastname: Ruff
authors.affiliation: Analytical Chemistry, Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Pöller
authors.affiliation: Analytical Chemistry, Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Alsaoub
authors.affiliation: Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknechtstrasse 24-25, D-14476, Golm/Potsdam, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Leimkühler
authors.affiliation: Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknechtstrasse 24-25, D-14476, Golm/Potsdam, Germany.
authors.forename: Ulla
authors.initials: U
authors.lastname: Wollenberger
authors.affiliation: Analytical Chemistry, Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany. Electronic address: wolfgang.schuhmann@ruhr-uni-bochum.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuhmann
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D001547:Benzaldehydes / Q000032:analysis / Q000378:metabolism; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D015374:Biosensing Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010640:Phenothiazines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D004800:Enzymes, Immobilized; D010640:Phenothiazines; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C100058:vanillin; D000445:Aldehyde Oxidoreductases; C031637:phenothiazine; D010100:Oxygen
keywords: Aldehyde oxidoreductase; Biofuel cell; Biosensor; Enzyme electrode; Phenothiazine; Redox polymer