title: Partitioning of carbon sources among functional pools to investigate short-term priming effects of biochar in soil: A (13)C study.
abstract: Biochar sequesters carbon (C) in soils because of its prolonged residence time, ranging from several years to millennia. In addition, biochar can promote indirect C-sequestration by increasing crop yield while, potentially, reducing C-mineralization. This laboratory study was set up to evaluate effects of biochar on C-mineralization with due attention to source appointment by using (13)C isotope signatures. An arable soil (S) (7.9 g organic C, OC kg(-1)) was amended (single dose of 10 g kg(-1) soil) with dried, grinded maize stover (leaves and stalks), either natural (R) or (13)C enriched (R*), and/or biochar (B/B*) prepared from the maize stover residues (450 °C). Accordingly, seven different combinations were set up (S, SR, SB, SR*, SB*, SRB*, SR*B) to trace the source of C in CO2 (180 days), dissolved organic-C (115 days) and OC in soil aggregate fractions (90 days). The application of biochar to soil reduced the mineralization of native soil organic C but the effect on maize stover-C mineralization was not consistent. Biochar application decreased the mineralization of the non-enriched maize stover after 90 days, this being consistent with a significant reduction of dissolved organic C concentration from 45 to 18 mg L(-1). However, no significant effect was observed for the enriched maize stover, presumably due to differences between the natural and enriched materials. The combined addition of biochar and enriched maize stover significantly increased (twofold) the presence of native soil organic C or maize derived C in the free microaggregate fraction relative to soil added only with stover. Although consistent effects among C sources and biochar materials remains elusive, our outcomes indicate that some biochar products can reduce mineralization and solubilization of other sources of C while promoting their physical protection in soil particles.
authors.affiliation: Department of Earth and Environmental Science, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Kerré
authors.affiliation: Department of Earth and Environmental Science, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium; The University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland 4072, Australia. Electronic address: m.hernandezsoriano@uq.edu.au.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Hernandez-Soriano
authors.affiliation: Department of Earth and Environmental Science, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
authors.forename: Erik
authors.initials: E
authors.lastname: Smolders
keywords: Biochar; Priming; Soil aggregates; Soil organic carbon; Stable isotopes