title: Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae).
abstract: In this study, different geographical populations of Rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. Phylogenetic trees were constructed using 12S and 16S rDNA sequences and showed two distinct clades: one composed of ticks from Brazil (Jaboticabal, SP), Cuba (Havana) Thailand (Bangkok) and the so-called "tropical strain" ticks. The second clade was composed of ticks from Spain (Zaragoza), Argentina (Rafaela, Santa Fe) and the so-called "temperate strain" ticks. Morphometric analysis showed good separation between females of the two clades and within the temperate clade. Males also exhibited separation between the two clades, but with some overlap. Multiple biological parameters revealed differences between the two clades, especially the weight of the engorged female. These results confirm the existence of at least two species under the name "R. sanguineus".
authors.affiliation: Instituto de Biociências, Departamento de Biologia, Unesp Campus Rio Claro, Av. 24 A, no. 1515, 13506-900 Rio Claro, SP, Brazil; Laboratório de Imunopatologia, Departamento de Patologia Veterinária, Unesp Campus Jaboticabal, Via de Acesso Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil.
authors.forename: Gustavo S
authors.initials: GS
authors.lastname: Sanches
authors.affiliation: Laboratório de Imunopatologia, Departamento de Patologia Veterinária, Unesp Campus Jaboticabal, Via de Acesso Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil.
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Évora
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, Santa Fe, Argentina.
authors.forename: Atílio J
authors.initials: AJ
authors.lastname: Mangold
authors.affiliation: Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
authors.forename: Sattaporn
authors.initials: S
authors.lastname: Jittapalapong
authors.affiliation: Centro de Ingeniería Genética y Biotecnología-CIGB, Habana, Cuba.
authors.forename: Alina
authors.initials: A
authors.lastname: Rodriguez-Mallon
authors.affiliation: Centro de Ingeniería Genética y Biotecnología-CIGB, Habana, Cuba.
authors.forename: Pedro E E
authors.initials: PE
authors.lastname: Guzmán
authors.affiliation: Laboratório de Imunopatologia, Departamento de Patologia Veterinária, Unesp Campus Jaboticabal, Via de Acesso Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil; Pontifícia Universidade Católica do Paraná-PUCPR, Rua Imaculada Conceição, 1155 Curitiba, PR, Brazil. Electronic address: gervasio.bechara@pucpr.br.
authors.forename: Gervásio H
authors.initials: GH
authors.lastname: Bechara
authors.affiliation: Instituto de Biociências, Departamento de Biologia, Unesp Campus Rio Claro, Av. 24 A, no. 1515, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Camargo-Mathias
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D005247:Feeding Behavior; D005260:Female; D008297:Male; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012098:Reproduction / Q000502:physiology; D048508:Rhipicephalus sanguineus / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D004247:DNA
keywords: Genetic analysis; Morphometry; Rhipicephalus sanguineus; Taxonomy