title: Microbubble-mediated ultrasound promotes accumulation of bone marrow mesenchymal stem cell to the prostate for treating chronic bacterial prostatitis in rats.
abstract: Chronic bacterial prostatitis (CBP) is an intractable disease. Although bone marrow mesenchymal stem cells (BMMSCs) are able to regulate inflammation in CBP, the effect of microbubble-mediated ultrasound- induced accumulation of BMMSCs on CBP remains unclear. To address this gap, a model of CBP was established in SD rats, which were then treated with BMMSCs alone (BMMSC group), BMMSCs with ultrasound (ultrasound group), BMMSCs with microbubble-mediated ultrasound (MMUS group) and compared with a healthy control group. A therapeutic-ultrasound apparatus was used to treat the prostate in the presence of circulating microbubbles and BMMSCs. The BMMSC distribution was assessed with in vivo imaging, and the prostate structure with light microscopy. Real-time quantitative RT-PCR, ELISA, and immunohistochemistry were used to assess the expressions of TNF-α and IL-1β. More BMMSCs were found in the prostate in the MMUS group than in the CBP, ultrasound, and BMMSC groups. Inflammatory cell infiltration, fibrous tissue hyperplasia, and tumor-like epithelial proliferation were significantly reduced in the MMUS group, as were the mRNA and protein expressions of TNF-α and IL-1β. Microbubble-mediated ultrasound-induced accumulation of BMMSCs can inhibit inflammation and decrease TNF-α and IL-1β expressions in the prostate of CBP rats, suggesting that this method may be therapeutic for CPB.
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Shanhong
authors.initials: S
authors.lastname: Yi
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.|Department of Urology, The 169th hospital of People's Liberation Army, Hengyang, Hunan Province, 421002, China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Han
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Department of Urology, Chongqing Emergency Medical Center, Chongqing, 400014, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Cui
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Shuangjiang
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liao
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ao
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Longkun
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D019793:Fluorescein / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D045423:Microbubbles; D011467:Prostate / Q000473:pathology*; D011472:Prostatitis / Q000382:microbiology* / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014465:Ultrasonics
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019793:Fluorescein