title: Synthesis of Isotopically Labeled (13)C3-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil.
abstract: The isotope dilution mass spectrometry (IDMS) is a highly efficient method for tackling the ion suppression in complex matrix by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), but a lack of commercial internal standards is a limiting factor for these analyses. Herein, an economical and efficient strategy for the synthesis of (13)C3-simazine via a three-step procedure was developed. The isotope-labeled internal standard was used for determination of simazine residue in soil samples. The quantitation method has a limit of detection of 0.015 μg/kg and quantitation of 0.08 μg/kg. The inter-day and intra-day precision of the method were below 4.6%. Recovery values were ranged between 92.9% and 99.2%. All the samples obtained from six provinces in China contained from 1 to 62 μg/kg of simazine.
authors.affiliation: Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China. songyan200714@163.com.|College of Pharmacology, Ningxia Medical University, Yinchuan 750004, China. songyan200714@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China. guoyangzhen163@163.com.
authors.forename: Yangzhen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China. zhangxia0561@126.com.|School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangxia0561@126.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China. 18311242015@163.com.|School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 18311242015@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China. chen@163.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. shegaimei@126.com.
authors.forename: Gaimei
authors.initials: G
authors.lastname: She
authors.affiliation: Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China. shedongmei@caas.cn.
authors.forename: Dongmei
authors.initials: D
authors.lastname: She
mesh_terms: D002247:Carbon Isotopes; D002853:Chromatography, Liquid; D004785:Environmental Pollutants / Q000032:analysis* / Q000138:chemical synthesis; D006540:Herbicides / Q000032:analysis* / Q000138:chemical synthesis; D006801:Humans; D007553:Isotope Labeling; D057230:Limit of Detection; D015588:Observer Variation; D012015:Reference Standards; D015203:Reproducibility of Results; D012839:Simazine / Q000032:analysis* / Q000138:chemical synthesis; D012987:Soil / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D002247:Carbon Isotopes; D004785:Environmental Pollutants; D006540:Herbicides; D012987:Soil; D012839:Simazine
keywords: 13C3-simazine; IDMS; UPLC-MS/MS; isotope labelling; synthesis