title: Development of a Genomic Resource and Quantitative Trait Loci Mapping of Male Calling Traits in the Lesser Wax Moth, Achroia grisella.
abstract: In the study of sexual selection among insects, the Lesser Waxmoth, Achroia grisella (Lepidoptera: Pyralidae), has been one of the more intensively studied species over the past 20 years. Studies have focused on how the male calling song functions in pair formation and on the quantitative genetics of male song characters and female preference for the song. Recent QTL studies have attempted to elucidate the genetic architecture of male song and female preference traits using AFLP markers. We continued these QTL studies using SNP markers derived from an EST library that allowed us to measure both DNA sequence variation and map loci with respect to the lepidopteran genome. We report that the level of sequence variation within A. grisella is typical among other Lepidoptera that have been examined, and that comparison with the Bombyx mori genome shows that macrosynteny is conserved. Our QTL map shows that a QTL for a male song trait, pulse-pair rate, is situated on the Z chromosome, a prediction for sexually selected traits in Lepidoptera. Our findings will be useful for future studies of genetic architecture of this model species and may help identify the genetics associated with the evolution of its novel acoustic communication.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas, United States of America.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Gleason
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas, United States of America.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, United States of America.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Hackett
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas, United States of America.
authors.forename: Bethany R
authors.initials: BR
authors.lastname: Harris
authors.affiliation: Institut de recherche sur la biologie de l'insecte (IRBI), CNRS UMR 7261,Université François Rabelais de Tours, Tours, France.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Greenfield
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D020224:Expressed Sequence Tags; D007178:Inbreeding; D008297:Male; D009036:Moths / Q000235:genetics* / Q000502:physiology; D040641:Quantitative Trait Loci; D012726:Sexual Behavior, Animal / Q000502:physiology; D014828:Vocalization, Animal / Q000502:physiology*