title: Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse.
abstract: Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classical Bordetella isolate from the trachea and lung of an ICR mouse. Isolate BH370 was initially identified as B. hinzii by 16S ribosomal DNA and ompA sequencing. Additionally, isolate BH370 also displayed antimicrobial sensitivity profiles similar to B. hinzii. However, analyses of nrdA sequences determined its identity as Bordetella genogroup 16. The isolation of BH370 from a healthy mouse suggests the possibility of it being a commensal. The nrdA gene was demonstrated to possess greater phylogenetic resolution as compared with 16S ribosomal DNA and ompA for the discrimination of non-classical Bordetella species.
authors.affiliation: Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Shih Keng
authors.initials: SK
authors.lastname: Loong
authors.forename: Nur Hidayana
authors.initials: NH
authors.lastname: Mahfodz
authors.forename: Haryanti Azura Mohamad
authors.initials: HA
authors.lastname: Wali
authors.forename: Siti Aisyah A
authors.initials: SA
authors.lastname: Talib
authors.forename: Siti Noraisah Ahmad
authors.initials: SN
authors.lastname: Nasrah
authors.forename: Pooi Fong
authors.initials: PF
authors.lastname: Wong
authors.forename: Sazaly
authors.initials: S
authors.lastname: Abubakar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001884:Bordetella / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010802:Phylogeny
chemical_list: D000900:Anti-Bacterial Agents