title: Uric acid quantification in fingernail of gout patients and healthy volunteers using HPLC-UV.
abstract: The presence of elevated uric acid (UA) levels is a sign of gout, that is, hyperuricemia. In this study the monitoring of the UA levels in less-invasive biological samples, such as the human fingernail, is suggested for the diagnosis and therapy of gout. Twenty-six healthy volunteers (HV) and 22 gout patients (GP) were studied. The UA was extracted from human fingernail samples, then separated on an Inertsil ODS-3 column (250 × 4.6 mm i.d., 4.0 μm, GL Sciences) by isocratic elution using methanol-74 mm phosphate buffer (pH 2.2) 2:98 (v/v). A UV detector was used to monitor the samples at 284 nm. Using the developed method, different UA concentrations were found in the GP and HV. When comparing the concentrations from GP with those from HV, a statistically significant correlation was observed between the UA (p < 0.01). In this study, the UA was confirmed as a potential biomarker for the diagnosis and therapy of gout. We have developed a novel sensitive, and simple method for the determination of UA in the fingernails of GP and HV. The human fingernail may serve as a noninvasive biosample for the diagnosis of gout. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory for Natural Resource of Changbai Mountain and Functional Molecules, Ministry of Education, College of Pharmacy, Yanbian University, Yanji, 133002, Jilin Province, China.
authors.forename: Xi-Ling
authors.initials: XL
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Affiliated Western Hospital, Yanbian University, Department of Endocrinology, Affiliated Hospital of Yanbian University, Yanji, 133000, Jilin, Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Pharmacy, Affiliated Western Hospital, Yanbian University, Department of Endocrinology, Affiliated Hospital of Yanbian University, Yanji, 133000, Jilin, Province, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Jin
authors.affiliation: Key Laboratory for Natural Resource of Changbai Mountain and Functional Molecules, Ministry of Education, College of Pharmacy, Yanbian University, Yanji, 133002, Jilin Province, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Li
authors.affiliation: Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Todoroki
authors.affiliation: Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Mizuno
authors.affiliation: Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Toyo'oka
authors.affiliation: Key Laboratory for Natural Resource of Changbai Mountain and Functional Molecules, Ministry of Education, College of Pharmacy, Yanbian University, Yanji, 133002, Jilin Province, China.|Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
authors.forename: Jun Zhe
authors.initials: JZ
authors.lastname: Min
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009262:Nails / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014527:Uric Acid / Q000032:analysis*
chemical_list: D014527:Uric Acid
keywords: HPLC-UV; fingernail; gout patient; uric acid