title: Identification and Expression Analyses of miRNAs from Two Contrasting Flower Color Cultivars of Canna by Deep Sequencing.
abstract: miRNAs are endogenous small RNA (sRNA) that play critical roles in plant development processes. Canna is an ornamental plant belonging to family Cannaceae. Here, we report for the first time the identification and differential expression of miRNAs in two contrasting flower color cultivars of Canna, Tropical sunrise and Red president. A total of 313 known miRNAs belonging to 78 miRNA families were identified from both the cultivars. Thirty one miRNAs (17 miRNA families) were specific to Tropical sunrise and 43 miRNAs (10 miRNA families) were specific to Red president. Thirty two and 18 putative new miRNAs were identified from Tropical sunrise and Red president, respectively. One hundred and nine miRNAs were differentially expressed in the two cultivars targeting 1343 genes. Among these, 16 miRNAs families targeting 60 genes were involved in flower development related traits and five miRNA families targeting five genes were involved in phenyl propanoid and pigment metabolic processes. We further validated the expression analysis of a few miRNA and their target genes by qRT-PCR. Transcription factors were the major miRNA targets identified. Target validation of a few randomly selected miRNAs by RLM-RACE was performed but was successful with only miR162. These findings will help in understanding flower development processes, particularly the color development in Canna.
authors.affiliation: Division of Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-National Botanical Research Institute campus, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
authors.forename: Sribash
authors.initials: S
authors.lastname: Roy
authors.affiliation: Division of Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-National Botanical Research Institute campus, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
authors.forename: Abhinandan Mani
authors.initials: AM
authors.lastname: Tripathi
authors.affiliation: Division of Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Division of Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.|Academy of Scientific and Innovative Research (AcSIR), CSIR-National Botanical Research Institute campus, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
authors.forename: Parneeta
authors.initials: P
authors.lastname: Mishra
authors.affiliation: Division of Plant Microbe Interaction, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Nautiyal
mesh_terms: D002338:Carotenoids / Q000032:analysis; D003116:Color; D005419:Flavonoids / Q000032:analysis; D035264:Flowers / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019862:Zingiberales / Q000235:genetics* / Q000254:growth & development
chemical_list: D005419:Flavonoids; D035683:MicroRNAs; D002338:Carotenoids