title: IL-27 attenuates the TGF-β1-induced proliferation, differentiation and collagen synthesis in lung fibroblasts.
abstract: OBJECTIVE
  Pulmonary fibrosis is a type of chronic lung disease and has characteristics that progress quickly, has a high fatality rate and a poor therapeutic effect. Our previous research showed that interleukin-27(IL-27) potentially attenuates BLM-induced pulmonary fibrosis, but the function of IL-27 in lung fibroblasts (LFs) differentiation pulmonary fibrosis is yet to be known.
  
  
  METHODS
  Here we investigated the effect of IL-27 on the proliferation, differentiation and collagen synthesis of lung fibroblasts induced by transforming growth factor-β1 (TGF-β1)using MTT, bromodeoxyuridine(BrdU) staining, real-time quantitative PCR(qPCR), Western blot, cell cycle FACS assay and immunofluorescence. We also examined the expression of the JAK/STAT and TGF-β1/Smad signaling pathway of IL-27 in lung fibroblasts.
  
  
  RESULTS
  TGF-β1 treated lung fibroblasts showed significantly increased proliferation, differentiation and collagen synthesis as well as overactivated JAK/STAT and TGF-β1/Smad signaling. However, the presence of IL-27 weakened these effects and obviously inactivated the JAK/STAT and TGF-β1/Smad signaling pathways.
  
  
  CONCLUSIONS
  Our results indicate that IL-27 may play an anti-fibrotic role in the development, differentiation and collagen synthesis in lung fibroblasts. These data also may provide a target gene therapy method in treating pulmonary fibrosis.
authors.affiliation: Department of Respiratory, The 2nd Affiliated Hospital of Kunming Medical University; Kunming, Yunnan, China. Electronic address: dongkm@hotmail.com.
authors.forename: Zhaoxing
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Radiology, The 2nd Affiliated Hospital of Kunming Medical University; Kunming, Yunnan, China. Electronic address: zhaoxinxiang06@126.com.
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, Yunnan Molecular Diagnostic Center, The 2nd Affiliated Hospital of Kunming Medical University, Dianmian Road, Kunming, Yunnan, China. Electronic address: 2820582685@qq.com.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Tai
authors.affiliation: Department of Respiratory, The 2nd Affiliated Hospital of Kunming Medical University; Kunming, Yunnan, China. Electronic address: 2233851218@qq.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Lei
authors.affiliation: Department of Respiratory, The 2nd Affiliated Hospital of Kunming Medical University; Kunming, Yunnan, China. Electronic address: w.y925@163.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiratory, The 2nd Affiliated Hospital of Kunming Medical University; Kunming, Yunnan, China. Electronic address: ljk5718@sina.com.cn.
authors.forename: ZhenKun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Respiratory, The 2nd Affiliated Hospital of Kunming Medical University; Kunming, Yunnan, China. Electronic address: ZT6958@sina.com.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D064094:Interleukin-27 / Q000494:pharmacology*; D053612:Janus Kinases / Q000378:metabolism; D008168:Lung / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D011658:Pulmonary Fibrosis / Q000188:drug therapy / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors*
chemical_list: D064094:Interleukin-27; D053773:Transforming Growth Factor beta1; D003094:Collagen; D053612:Janus Kinases
keywords: Differentiation; Interleukin-27; LFs; Proliferation; TGF-β1