title: Novel chimeric foot-and-mouth disease virus-like particles harboring serotype O VP1 protect guinea pigs against challenge.
abstract: Foot-and-mouth disease is a highly contagious, acute viral disease of cloven-hoofed animal species causing severe economic losses worldwide. Among the seven serotypes of foot-and-mouth disease virus (FMDV), serotype O is predominant, but its viral capsid is more acid sensitive than other serotypes, making it more difficult to produce empty serotype O VLPs in the low pH insect hemolymph. Therefore, a novel chimeric virus-like particle (VLP)-based candidate vaccine for serotype O FMDV was developed and characterized in the present study. The chimeric VLPs were composed of antigenic VP1 from serotype O and segments of viral capsid proteins from serotype Asia1. These VLPs elicited significantly higher FMDV-specific antibody levels in immunized mice than did the inactivated vaccine. Furthermore, the chimeric VLPs protected guinea pigs from FMDV challenge with an efficacy similar to that of the inactivated vaccine. These results suggest that chimeric VLPs have the potential for use in vaccines against serotype O FMDV infection.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China. Electronic address: lizhiyong02@caas.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Yinli
authors.initials: Y
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Xingcai
authors.initials: X
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Xingwen
authors.initials: X
authors.lastname: Bai
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors.forename: Youji
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000517:prevention & control* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D015967:Gene Expression Regulation, Viral; D006168:Guinea Pigs; D051379:Mice; D046529:Microscopy, Electron, Transmission; D011897:Random Allocation; D061987:Sf9 Cells; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D058425:Vaccines, Virus-Like Particle
keywords: Chimeric VLP; FMDV; Serotype O; Virus-like particles