title: MicroRNA-200a-3p suppresses tumor proliferation and induces apoptosis by targeting SPAG9 in renal cell carcinoma.
abstract: Sperm-associated antigen 9(SPAG9), as a well-recognized oncogene protein, has a critical effect on renal cell carcinoma (RCC) progression. Our study tried to explore the mediator of miR-200a-3p, a tumor suppressing miRNA on SPAG9 expression and renal cell proliferation and apoptosis. We found the expression of miR-200a-3p was significantly lower in RCC specimens. Based on in vitro assays, we found miR-200a-3p significantly inhibit cancer cell proliferation by inducing apoptosis. In addition, our study uncovered that miR-200a-3p directly regulates oncogenic SPAG9 in 786-O and ACHN cells. Silencing of SPAG9 resulted in significantly decreased in the growth and the cell cycle of the renal cancer cell lines. Understanding of oncogenic SPAG9 regulated by miR-200a-3p might be beneficial to reveal new therapeutic targets for RCC.
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Xian
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China. Electronic address: guxqprofessor@163.com.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Gu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; C111047:SPAG9 protein, human
keywords: Apoptosis; Proliferation; Renal cell carcinoma; SPAG9; miR-200a-3p