title: Treatment options after virological failure of first-line tenofovir-based regimens in South Africa: an analysis by deep sequencing.
abstract: In a South African cohort of participants living with HIV developing virological failure on first-line tenofovir disoproxyl fumarate (TDF)-based regimens, at least 70% of participants demonstrated TDF resistance according to combined Sanger and MiSeq genotyping. Sanger sequencing missed the K65R mutation in 30% of samples. Unless HIV genotyping is available to closely monitor epidemiological HIV resistance to TDF, its efficacy as second-line therapy will be greatly compromised.
authors.affiliation: aIrsiCaixa AIDS Research Institute, Universitat Autònoma de Barcelona, Badalona, Catalonia bUniversitat de Vic-Central de Catalunya, Vic, Spain cMcCord Hospital dUniversity of KwaZulu-Natal eNelson Mandela School of Medicine, Durban, South Africa fDivision of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts gRollins School of Public Health, Emory University hEmory University School of Medicine, Atlanta, Georgia, USA iHIV Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Catalonia, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Casadellà
authors.forename: Marc
authors.initials: M
authors.lastname: Noguera-Julian
authors.forename: Henry
authors.initials: H
authors.lastname: Sunpath
authors.forename: Michelle
authors.initials: M
authors.lastname: Gordon
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodriguez
authors.forename: Mariona
authors.initials: M
authors.lastname: Parera
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Kuritzkes
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Marconi
authors.forename: Roger
authors.initials: R
authors.lastname: Paredes
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology / Q000627:therapeutic use*; D023241:Antiretroviral Therapy, Highly Active / Q000379:methods; D024882:Drug Resistance, Viral; D005838:Genotype; D060005:Genotyping Techniques; D006678:HIV / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008826:Microbial Sensitivity Tests; D013019:South Africa; D000068698:Tenofovir / Q000494:pharmacology / Q000627:therapeutic use*; D017211:Treatment Failure
chemical_list: D019380:Anti-HIV Agents; D000068698:Tenofovir