title: The anti-apoptosis effect of MLAA-34 in leukemia and the β-catenin/T cell factor 4 protein pathway.
abstract: Objective This study aims to observe the effects of MLAA-34 gene in leukemia and explore its mechanism. Methods MLAA-34 RNAi vector was constructed. NOD/SCID mice and 293T cells, K562 cells, HL60 cells and U937 cells were used in this study. They were divided into MLAA-34-siRNA group and control group. The proliferation ability and apoptosis of cells were detected. The expression levels of β-Catenin and TCF 4 were determined using western blotting and immunohistochemical methods. Results There was significant correlation between MLAA-34 gene and U937 cell proliferation, the apoptosis rates of U937 cells with siRNA infection were higher than that of control group. There were significant differences in the expression levels of β-Catenin and TCF 4 between U937 cells and U937 with MLAA-34-siRNA cells group. Conclusions MLAA-34 gene had anti-apoptotic effect in leukemia, which maybe through the β-Catenin/TCF 4 pathway.
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University School of MedicineThe West Five Road, 157#, Xi'an 710004, Shaanxi, China; Department of Medicine, The Fourth Hospital of Xi'anNo. 21 Jiefang Road, Shaanxi 710004, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University School of Medicine The West Five Road, 157#, Xi'an 710004, Shaanxi, China.
authors.forename: Wanggang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University School of Medicine The West Five Road, 157#, Xi'an 710004, Shaanxi, China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University School of Medicine The West Five Road, 157#, Xi'an 710004, Shaanxi, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lei
authors.affiliation: Department of Gynaecology and Obstetrics, The Fourth Hospital of Xi'an No. 21 Jiefang Road, Shaanxi 710004, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicine, The Fourth Hospital of Xi'an No. 21 Jiefang Road, Shaanxi 710004, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University School of Medicine The West Five Road, 157#, Xi'an 710004, Shaanxi, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University School of Medicine The West Five Road, 157#, Xi'an 710004, Shaanxi, China.
authors.forename: Aili
authors.initials: A
authors.lastname: He
keywords: MLAA-34; apoptosis; leukemia; β-Catenin/TCF 4 pathway