title: Purification, characterization and antiglycation activity of a novel polysaccharide from black currant.
abstract: A novel polysaccharide fraction (BCP-1) was extracted from the black currant fruit by ultrasound-assisted compound enzyme and purified by chromatography on macroporous resin D4006, anion-exchange Q-Sepharose FF and Sephadex G-100 columns. BCP-1 consisted of galacturonic acid, xylose, mannose, glucose and galactose in a ratio of 1.00:3.14:1.83:17.90:1.98 and its molecular weight was 14,050 Da. The preliminary structure features of BCP-1 were investigated by FT-IR and NMR. SEM and Congo red test showed that BCP-1 had honeycomb-like structure, but no triple helix structure. BCP-1 exhibited significant inhibitory abilities on protein glycation. Especially, BCP-1 showed obvious inhibitory effects on the formation of dicarbonyl compounds and AGEs (% inhibition of 66.95 ± 0.33% and 67.15 ± 0.40% respectively), but weaker inhibition Amadori compound production (max. 37.15 ± 0.36% inhibition). This suggested that the inhibitory action of BCP-1 on protein glycation was more effective on the later phases of dicarbonyl compounds and AGEs formation.
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Gaijie
authors.initials: G
authors.lastname: Liu
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: yangyu_002@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dai
mesh_terms: D005638:Fruit / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D031965:Ribes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D011134:Polysaccharides
keywords: Antiglycation activity; Black currant; Carbazole (PubChem CID: 6854); Characterization; Girard-T (PubChem CID: 67156); Polysaccharides; Purification; Trifluoroacetic acid (PubChem CID: 6422); d-Arabinose (PubChem CID: 66308); d-Fructose (PubChem CID: 5984); d-Fucose (PubChem CID: 94270); d-Galactose (PubChem CID: 6036); d-Galacturonic acid (PubChem CID: 439215); d-Glucose (PubChem CID: 5793); d-Mannose (PubChem CID: 18950); d-Rhamnose (PubChem CID: 439548)