title: The FgNot3 Subunit of the Ccr4-Not Complex Regulates Vegetative Growth, Sporulation, and Virulence in Fusarium graminearum.
abstract: The Ccr4-Not complex is evolutionarily conserved and important for multiple cellular functions in eukaryotic cells. In this study, the biological roles of the FgNot3 subunit of this complex were investigated in the plant pathogenic fungus Fusarium graminearum. Deletion of FgNOT3 resulted in retarded vegetative growth, retarded spore germination, swollen hyphae, and hyper-branching. The ΔFgnot3 mutants also showed impaired sexual and asexual sporulation, decreased virulence, and reduced expression of genes related to conidiogenesis. Fgnot3 deletion mutants were sensitive to thermal stress, whereas NOT3 orthologs in other model eukaryotes are known to be required for cell wall integrity. We found that FgNot3 functions as a negative regulator of the production of secondary metabolites, including trichothecenes and zearalenone. Further functional characterization of other components of the Not module of the Ccr4-Not complex demonstrated that the module is conserved. Each subunit primarily functions within the context of a complex and might have distinct roles outside of the complex in F. graminearum. This is the first study to functionally characterize the Not module in filamentous fungi and provides novel insights into signal transduction pathways in fungal development.
authors.affiliation: Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University, Seoul, Republic of Korea.
authors.forename: Duc-Cuong
authors.initials: DC
authors.lastname: Bui
authors.affiliation: Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University, Seoul, Republic of Korea.|Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hokyoung
authors.initials: H
authors.lastname: Son
authors.affiliation: Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Kim
authors.affiliation: Eco-friendly New Materials Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
authors.forename: Hun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Eco-friendly New Materials Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
authors.forename: Gyung Ja
authors.initials: GJ
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yin-Won
authors.initials: YW
authors.lastname: Lee
mesh_terms: D004875:Ergosterol / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D005670:Fusarium / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics; D025301:Hyphae / Q000254:growth & development*; D009183:Mycotoxins / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D013172:Spores, Fungal / Q000254:growth & development*
chemical_list: D005656:Fungal Proteins; D009183:Mycotoxins; D004875:Ergosterol