title: Green Tea Polyphenols Attenuated Glutamate Excitotoxicity via Antioxidative and Antiapoptotic Pathway in the Primary Cultured Cortical Neurons.
abstract: Green tea polyphenols are a natural product which has antioxidative and antiapoptotic effects. It has been shown that glutamate excitotoxicity induced oxidative stress is linked to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. In this study we explored the neuroprotective effect of green teen polyphenols against glutamate excitotoxicity in the primary cultured cortical neurons. We found that green tea polyphenols protected against glutamate induced neurotoxicity in the cortical neurons as measured by MTT and TUNEL assays. Green tea polyphenols were then showed to inhibit the glutamate induced ROS release and SOD activity reduction in the neurons. Furthermore, our results demonstrated that green tea polyphenols restored the dysfunction of mitochondrial pro- or antiapoptotic proteins Bax, Bcl-2, and caspase-3 caused by glutamate. Interestingly, the neuroprotective effect of green tea polyphenols was abrogated when the neurons were incubated with siBcl-2. Taken together, these results demonstrated that green tea polyphenols protected against glutamate excitotoxicity through antioxidative and antiapoptotic pathways.
authors.affiliation: Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, China; Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cong
authors.affiliation: Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, China; College of Life & Environmental Science, Minzu University of China, Beijing 100081, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Cao
authors.affiliation: Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yong
authors.identifier: 0000-0002-7529-4408
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, China; College of Life & Environmental Science, Minzu University of China, Beijing 100081, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Qin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology*; D018698:Glutamic Acid / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D000066829:Neuroprotection / Q000187:drug effects; D009498:Neurotoxins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009498:Neurotoxins; D059808:Polyphenols; D013662:Tea; D051028:bcl-2-Associated X Protein; D018698:Glutamic Acid; D053148:Caspase 3