title: Expression of the Receptor for Advanced Glycation End Products in Epicardial Fat: Link with Tissue Thickness and Local Insulin Resistance in Coronary Artery Disease.
abstract: Increased expression of receptor for advanced glycation end products (RAGE) in adipose tissue has been associated with inflammation, adipocyte hypertrophy, and impaired insulin signal. Epicardial adipose tissue (EAT), a visceral fat surrounding the myocardium, is potentially involved in the onset/progression of coronary artery disease (CAD). To date, the role of RAGE in EAT has not been explored much. We examined whether the RAGE expression in EAT was associated with EAT adiposity and metabolic dysfunctions normally found in CAD patients. EAT samples were obtained from 33 patients undergoing open-heart surgery. EAT expression of RAGE, GLUT4, adiponenctin, GLO1, HMGB1, TLR-4, and MyD88 was analyzed by microarray. EAT thickness was quantified by echocardiography. Anthropometric measures and clinical parameters were taken. BMI, HOMA-IR, and LAP indices were calculated. With increasing RAGE expression in EAT we observed increases in EAT thickness, reduced expression of GLUT4, adiponectin, and GLO1, and elevations of HMGB1, TLR-4, and MyD88. There were significant correlations between RAGE and EAT thickness and between RAGE and the genes. LAP was higher in patients with increased RAGE expression. Our data suggest that in CAD patients RAGE may be involved in promoting EAT adiposity and metabolic dysfunction, such as impaired insulin signaling.
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Via L. Mangiagalli 31, 20133 Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Dozio
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Via L. Mangiagalli 31, 20133 Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Vianello
authors.affiliation: Diabetology and Metabolic Disease Unit, I.R.C.C.S. Policlinico San Donato, Piazza E. Malan 1, 20097 San Donato Milanese, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Briganti
authors.affiliation: Randox Laboratories Ltd., R&D, 55 Diamond Road, Crumlin, Antrim, Belfast BT29 4QY, UK.
authors.forename: John
authors.initials: J
authors.lastname: Lamont
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Via L. Mangiagalli 31, 20133 Milan, Italy.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Tacchini
authors.affiliation: Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Schmitz
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Via L. Mangiagalli 31, 20133 Milan, Italy; Service of Laboratory Medicine 1-Clinical Pathology, I.R.C.C.S. Policlinico San Donato, Piazza E. Malan 1, 20097 San Donato Milanese, Italy.
authors.forename: Massimiliano Marco
authors.initials: MM
authors.lastname: Corsi Romanelli
mesh_terms: D050154:Adiposity; D000368:Aged; D000369:Aged, 80 and over; D003324:Coronary Artery Disease / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D024243:HMGB1 Protein / Q000032:analysis; D006801:Humans; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000503:physiopathology; D007791:Lactoylglutathione Lyase / Q000032:analysis; D008297:Male; D008875:Middle Aged; D053594:Myeloid Differentiation Factor 88 / Q000032:analysis; D010496:Pericardium / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000503:physiopathology; D000067759:Receptor for Advanced Glycation End Products / Q000032:analysis* / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000032:analysis; D014463:Ultrasonography; D015854:Up-Regulation
chemical_list: C000593871:AGER protein, human; D024243:HMGB1 Protein; C585491:HMGB1 protein, human; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; D000067759:Receptor for Advanced Glycation End Products; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; C000597196:GLO1 protein, human; D007791:Lactoylglutathione Lyase