title: Colorectal cancer cell-derived interleukin-6 enhances the phagocytic capacity and migration of THP-1 cells.
abstract: Macrophages perform a versatile range of functions in response to environmental stimuli. In the present study, we evaluated whether interleukin-6 (IL-6), a cytokine released from colorectal cancer (CRC) cells and associated with CRC pathogenesis and metastasis, modulates the phagocytic capacity and migratory ability of macrophages, using a monocyte-macrophage THP-1 cell model and human peripheral monocytes. We found that CRC cells enhanced the phagocytic capacity and migration of THP-1 cells and human peripheral monocytes. CRC cell culture supernatants and recombinant IL-6 neutralized with anti-IL-6 and anti-gp130 antibodies considerably decreased IL-6-mediated phagocytosis by and migration of THP-1 cells and human peripheral monocytes, via the phosphorylation of signal transducer and activator of transcription 3 (STAT3). Our data suggest that CRC cells secreting IL-6 via STAT3 phosphorylation can enhance the phagocytic capacity and migration of macrophages in the tumor microenvironment.
authors.affiliation: Division of Hemato-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan. Electronic address: yehtyng@gmail.com.
authors.forename: Kun-Yun
authors.initials: KY
authors.lastname: Yeh
authors.affiliation: Division of Hemato-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan.
authors.forename: Tsung-Han
authors.initials: TH
authors.lastname: Wu
authors.affiliation: Division of Hemato-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung & Chang Gung University, College of Medicine, Keelung, Taiwan.
authors.forename: Tai-Ling
authors.initials: TL
authors.lastname: Wu
mesh_terms: D000906:Antibodies / Q000276:immunology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D050822:Cytokine Receptor gp130 / Q000276:immunology; D004789:Enzyme Activation / Q000276:immunology; D019073:HT29 Cells; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology* / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D059016:Tumor Microenvironment / Q000276:immunology
chemical_list: D000906:Antibodies; D017077:Culture Media, Conditioned; C508600:IL6 protein, human; D015850:Interleukin-6; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D050822:Cytokine Receptor gp130
keywords: Colorectal cancer; Interleukin-6; Macrophages; Migration; Phagocytosis