title: Zfrp8/PDCD2 Interacts with RpS2 Connecting Ribosome Maturation and Gene-Specific Translation.
abstract: Zfrp8/PDCD2 is a highly conserved protein essential for stem cell maintenance in both flies and mammals. It is also required in fast proliferating cells such as cancer cells. Our previous studies suggested that Zfrp8 functions in the formation of mRNP (mRNA ribonucleoprotein) complexes and also controls RNA of select Transposable Elements (TEs). Here we show that in Zfrp8/PDCD2 knock down (KD) ovaries, specific mRNAs and TE transcripts show increased nuclear accumulation. We also show that Zfrp8/PDCD2 interacts with the (40S) small ribosomal subunit through direct interaction with RpS2 (uS5). By studying the distribution of endogenous and transgenic fluorescently tagged ribosomal proteins we demonstrate that Zfrp8/PDCD2 regulates the cytoplasmic levels of components of the small (40S) ribosomal subunit, but does not control nuclear/nucleolar localization of ribosomal proteins. Our results suggest that Zfrp8/PDCD2 functions at late stages of ribosome assembly and may regulate the binding of specific mRNA-RNPs to the small ribosomal subunit ultimately controlling their cytoplasmic localization and translation.
authors.affiliation: Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Minakhina
authors.affiliation: Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Naryshkina
authors.affiliation: Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
authors.forename: Neha
authors.initials: N
authors.lastname: Changela
authors.affiliation: Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
authors.forename: William
authors.initials: W
authors.lastname: Tan
authors.affiliation: Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
authors.forename: Ruth
authors.initials: R
authors.lastname: Steward
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D029721:Drosophila Proteins; D012269:Ribosomal Proteins; C522153:Zfrp8 protein, Drosophila; C030004:ribosomal protein S2