title: Taurine protects against As2O3-induced autophagy in pancreas of rat offsprings through Nrf2/Trx pathway.
abstract: Arsenic was increasingly to blame as a risk factor for type 2 diabetes mellitus. In our previous study, we had found iAs stimulated autophagic flux and caused autophagic cell death through ROS pathway in INS-1 cells. Since NF-E2-related factor 2 (Nrf2) and the thioredoxin (Trx) system was a crucial line of defense against ROS, we investigated whether Nrf2/Trx pathway contributed to As2O3-stimulated autophagy and the role of taurine in this study. After treatment with 2 mg/kg BW-8 mg/kg BW As2O3 for 57 d, the expression of Nrf2 protein was decreased significantly in offsprings' pancreas. The expression of Trx gene was decreased significantly in pancreas subsequently. Finally, the generation of reactive oxygen species stimulated autophagy in arsenic-treated pancreas. Taurine could reverse arsenic-inhibited Nrf2 and Trx and inhibit autophagy. In short, inhibition of Nrf2/Trx pathway might play an important role in the pathogenesis of arsenic-related diabetes. Taurine could serve as nutrition supplementation against arsenic-related diabetes in high arsenic exposure area.
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yao
authors.affiliation: Liaoning Anti-Degenerative Diseases Natural Products Engineering Research Center, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Tianming
authors.initials: T
authors.lastname: Qiu
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Baoxu
authors.initials: B
authors.lastname: Qi
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Liaoning Anti-Degenerative Diseases Natural Products Engineering Research Center, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Liaoning Anti-Degenerative Diseases Natural Products Engineering Research Center, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Liaoning Anti-Degenerative Diseases Natural Products Engineering Research Center, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lvshun South Road, Dalian 116044, PR China. Electronic address: sunxiance@aliyun.com.
authors.forename: Xiance
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals; D001343:Autophagy / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D010087:Oxides / Q000633:toxicity*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013654:Taurine / Q000494:pharmacology*; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001152:Arsenicals; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D010087:Oxides; D013654:Taurine; D008315:Malondialdehyde; D013879:Thioredoxins; D000077237:Arsenic Trioxide
keywords: As(2)O(3); Autophagy; Nrf2; Taurine; Trx