title: Semaphorin 3A attenuates cardiac autonomic disorders and reduces inducible ventricular arrhythmias in rats with experimental myocardial infarction.
abstract: BACKGROUND
  To investigate the effects of semaphorin 3A (sema 3A) on cardiac autonomic regulation and subsequent ventricular arrhythmias (VAs) in post-infarcted hearts.
  
  
  RESULTS
  In order to explore the functions of sema 3A in post-infarcted hearts, lentivirus-Sema 3A-shRNA and negative control vectors were delivered to the peri-infarcted myocardium rats respectively. Meanwhile, recombinant sema 3A and control (0.9% NaCl solution) were injected intravenously into infarcted rats to test the therapeutic potential of sema 3A. Results indicated that levels of sema 3A were higher in post-infarcted hearts compared with sham rats. However, sema 3A silencing leaded to sympathetic hyperinnervation, increased myocardial norepinephrine (NE) content and inducible VAs. Conversely, the intravenous administration of sema 3A to infarcted rats reduced sympathetic nerve sprouting, improved cardiac autonomic regulation, and decreased the incidence of inducible VAs. However, both infarct size and cardiac function were similar among infarcted hearts.
  
  
  CONCLUSIONS
  The upregulation and administration of sema 3A exerted beneficial effects on infarction-induced cardiac autonomic disorders by increasing cardiac electrical stability and reducing VAs. Sema 3A might be a potential therapeutic agent for cardiac autonomic abnormalities induced arrhythmias.
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. hyperhhs@163.com.
authors.forename: Hesheng
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. 57467238@qq.com.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Xuan
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. xuemei2575@126.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. chengwenjuan12@163.com.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. yeziwang812@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. xinran0207@126.com.
authors.forename: Xinran
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. yjangelsmile@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. lixiaolu006@163.com.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. emilynana.hi@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. 497972404@qq.com.
authors.forename: Yugen
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, 250014, Jinan, China. yansuhua5537@163.com.
authors.forename: Suhua
authors.initials: S
authors.lastname: Yan
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004562:Electrocardiography; D055785:Gene Knockdown Techniques; D006321:Heart / Q000187:drug effects* / Q000294:innervation / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D040121:Semaphorin-3A / Q000235:genetics* / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D017180:Tachycardia, Ventricular / Q000235:genetics* / Q000503:physiopathology; D014693:Ventricular Fibrillation / Q000235:genetics* / Q000503:physiopathology
chemical_list: D011994:Recombinant Proteins; C488218:Sema3a protein, rat; D040121:Semaphorin-3A; D009638:Norepinephrine