title: The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival.
abstract: The aim of this study was to explore the protective effects of ulinastatin (urinary trypsin inhibitor, UTI) on liver ischemia-reperfusion injury (IRI) and graft survival. We employed mouse liver cold IRI and orthotopic liver transplantation (OLTx) models. UTI was added to lactated Ringer's (LR) solution for liver perfusion and preservation in vitro or combined with UTI injection intraperitoneally to the liver graft recipient. Our results indicated that UTI supplementation protected the liver from cold IRI in a dose-dependent manner and prolonged liver graft survival from extended cold preserved liver donors significantly. The underlying mechanism of UTI on liver IRI may be mediated by inhibition of proinflammatory cytokine release, increasing the expression of the antiapoptotic gene Bcl-2 and decreasing the expression of the proapoptosis genes of Caspase-3 and Bax, and further protects hepatocytes from apoptotic death and improves liver function.
authors.affiliation: Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Lianyue
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China; Department of Liver Surgery, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Zhongshan Hospital of Fudan University, Shanghai 200032, China.
authors.forename: Peiyao
authors.initials: P
authors.lastname: Fu
authors.affiliation: Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Zhuonan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Tumor Therapy, Tumor Hospital of Jilin Province, Changchun 130033, China.
authors.forename: Peidong
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Mingang
authors.initials: M
authors.lastname: Xin
authors.affiliation: Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Zhanpeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Wei
authors.identifier: 0000-0001-7917-7666
authors.initials: W
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D003080:Cold Temperature; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006085:Graft Survival; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D016031:Liver Transplantation; D008297:Male; D008810:Mice, Inbred C57BL; D010477:Perfusion; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D012996:Solutions; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D020011:Protective Agents; D012996:Solutions; D014361:Trypsin Inhibitors; D051028:bcl-2-Associated X Protein; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D053148:Caspase 3