title: Fate of PLA and PCL-Based Polymeric Nanocarriers in Cellular and Animal Models of Triple-Negative Breast Cancer.
abstract: An integrated platform to assess the interaction between nanocarriers and biological matrices has been developed by our group using poly methyl-methacrylate nanoparticles. In this study, we exploited this platform to evaluate the behavior of two biodegradable formulations, poly-ε-caprolactone (PCL3) and poly lactic-acid (PLA8), respectively, in cellular and animal models of triple-negative breast cancer (TNBC). Both NPs shared the main physicochemical parameters (size, shape, ζ-potential) and exclusively differentiated on the material on which they are composed. Our results showed that (1) PLA8 NPs, systemically injected in mice, underwent rapid degradation without penetration into tumors; (2) PLA8 NPs were not internalized in the human TNBC cell line (MDA-MB-231); (3) PCL3 NPs had a longer bioavailability, reached the tumor parenchyma, and efficiently penetrated in MDA-MB-231 cells. Our data highlight the relevance of the material selection to both improve bioavailability and target tropism, and make PCL3 NPs an interesting tool for the development of nanodrugs against TNBC.
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Sitia
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich , CH-8093 Zurich, Switzerland.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Ferrari
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Martina B
authors.initials: MB
authors.lastname: Violatto
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Laura
authors.initials: L
authors.lastname: Talamini
authors.affiliation: Dipartimento di Chimica Materiali e Ingegneria Chimica "G. Natta", Politecnico di Milano , 20133 Milano, Italia.
authors.forename: Luca
authors.initials: L
authors.lastname: Dragoni
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich , CH-8093 Zurich, Switzerland.
authors.forename: Claudio
authors.initials: C
authors.lastname: Colombo
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Laura
authors.initials: L
authors.lastname: Colombo
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Monica
authors.initials: M
authors.lastname: Lupi
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Paolo
authors.initials: P
authors.lastname: Ubezio
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Maurizio
authors.initials: M
authors.lastname: D'Incalci
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich , CH-8093 Zurich, Switzerland.
authors.forename: Massimo
authors.initials: M
authors.lastname: Morbidelli
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Mario
authors.initials: M
authors.lastname: Salmona
authors.affiliation: Dipartimento di Chimica Materiali e Ingegneria Chimica "G. Natta", Politecnico di Milano , 20133 Milano, Italia.
authors.forename: Davide
authors.initials: D
authors.lastname: Moscatelli
authors.affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , 20156 Milano, Italia.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bigini
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D051379:Mice; D053769:Nanocapsules / Q000009:adverse effects / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D014018:Tissue Distribution; D064726:Triple Negative Breast Neoplasms / Q000188:drug therapy*
chemical_list: D004337:Drug Carriers; D053769:Nanocapsules; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide)