title: ApoE and S-100 expression and its significance in the brain tissue of rats with focal contusion.
abstract: This study explored the effect of focal cerebral contusion on the expression of ApoE and S-100, and its significance in determining the time of brain injury. Based on a rat model of cerebral contusion, immunohistochemistry was used to analyze the expressions of S-100 and ApoE at different time points after injury. Thirty minutes following cerebral contusion, ApoE protein expression was significantly increased in cortex neurons (P < 0.01), and S-100 protein expression was significantly (P < 0.001) elevated 2 h after cerebral contusion. Over time, the number of ApoE and S-100 positively expressing cells gradually increased. Three days after injury, ApoE was widely distributed throughout the tissue and the number of ApoE-positive cells and staining intensity reached a peak. ApoE expression decreased after this time point. Five days after cerebral contusion, the number of S-100-positive cells reached a peak level of expression higher than that in the control group. Our data demonstrate that the expression of ApoE and S-100 correlated with the progression of focal cerebral contusion. This suggests that both proteins may serve as effective biomarkers of focal cerebral contusions.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Critical Care Medicine Department, the First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: R F
authors.initials: RF
authors.lastname: Chai
authors.affiliation: Department of Pathology, Successful Hospital Affiliated to Xiamen University, Xiamen, Fujian Province, China.
authors.forename: W S
authors.initials: WS
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: H
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, Xinjiang Province, China.
authors.forename: G
authors.initials: G
authors.lastname: Dangmurenjiafu
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; D009418:S100 Proteins