title: SGLT1 sugar transporter/sensor is required for post-oral glucose appetition.
abstract: Recent findings suggest that the intestinal sodium-glucose transporter 1 (SGLT1) glucose transporter and sensor mediates, in part, the appetite-stimulation actions of intragastric (IG) glucose and nonmetabolizable α-methyl-d-glucopyranoside (MDG) infusions in mice. Here, we investigated the role of SGLT1 in sugar conditioning using SGLT1 knockout (KO) and C57BL/6J wild-type (WT) mice. An initial experiment revealed that both KO and WT mice maintained on a very low-carbohydrate diet display normal preferences for saccharin, which was used in the flavored conditioned stimulus (CS) solutions. In experiment 2, mice were trained to drink one flavored solution (CS+) paired with an IG MDG infusion and a different flavored solution (CS-) paired with IG water infusion. In contrast to WT mice, KO mice decreased rather than increased the intake of the CS+ during training and failed to prefer the CS+ over the CS- in a choice test. In experiment 3, the KO mice also decreased their intake of a CS+ paired with IG glucose and avoided the CS+ in a choice test, unlike WT mice, which preferred the CS+ to CS-. In experiment 4, KO mice, like WT mice preferred a glucose + saccharin solution to a saccharin solution. These findings support the involvement of SGLT1 in post-oral glucose and MDG conditioning. The results also indicate that sugar malabsorption in KO mice has inhibitory effects on sugar intake but does not block their natural preference for sweet taste.
authors.affiliation: Department of Psychology, Brooklyn College, City University of New York, Brooklyn, New York; and Cognition, Brain, and Behavior Doctoral Subprogram, Graduate School, City University of New York, New York, New York; and asclafani@gc.cuny.edu.
authors.forename: Anthony
authors.initials: A
authors.lastname: Sclafani
authors.affiliation: Department of Molecular Plant Physiology and Biophysics, Julius-von-Sachs-Institute, University Würzburg, Würzburg, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Koepsell
authors.affiliation: Department of Psychology, Brooklyn College, City University of New York, Brooklyn, New York; and Cognition, Brain, and Behavior Doctoral Subprogram, Graduate School, City University of New York, New York, New York; and.
authors.forename: Karen
authors.identifier: http://orcid.org/0000-0002-1442-7692
authors.initials: K
authors.lastname: Ackroff
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001066:Appetite / Q000502:physiology*; D066249:Craving / Q000502:physiology*; D019422:Dietary Sucrose / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism*
chemical_list: D019422:Dietary Sucrose; C495675:Slc5a1 protein, mouse; D051269:Sodium-Glucose Transporter 1; D005947:Glucose
keywords: SGLT1 knockout; glucose; post-oral sugar conditioning; α-methyl-d-glucopyranoside