title: The regulation of transcriptome responses in zebrafish embryo exposure to triadimefon.
abstract: The residue of triadimefon (TDF) (a pesticide) has become the pollutant in water due to its intensive use in agriculture and medicine, and its stability in water leaching from soil and vegetation. In this study, RNA-seq, a high-throughput method was performed, to analyze the global expression of differential expressed genes (DEGs) in zebrafish embryos treated with TDF (10 μg/mL) from fertilization to 72 h post-fertilization (hpf) as compared with that in the control group (without TDF treatment). Two cDNA libraries were generated from treated and non-treated embryos, respectively. With the 79.4% and 78.8% of reads mapped to the reference, it was observed that many differential genes were expressed between the two libraries. The most 20 differentially expressed up-regulated or down-regulated genes were involving in the signaling transduction, the activation of many genes related to cytochrome P450 enzymes, and molecular metabolism. Validation of seven genes expression confirmed RNA-seq results. The transcriptome sequences were further subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and showed diverse biological functions and metabolic pathways. The data from this study contributed to a better understanding of the potential consequences of fish exposed to TDF, and to evaluate the potential threat of TDF to fish population in the aquatic environment. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 217-226, 2017.
authors.affiliation: Institute of Biochemistry, Microbiology, Immunology, Chung Shan Medical University, Taichung, Taiwan.|Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Li-Sung
authors.initials: LS
authors.lastname: Hsu
authors.affiliation: Department of Life Sciences, National Central University, Jhongli, Taiwan.
authors.forename: Bin-Hao
authors.initials: BH
authors.lastname: Chiou
authors.affiliation: Institute of Biochemistry, Microbiology, Immunology, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Tung-Wei
authors.initials: TW
authors.lastname: Hsu
authors.affiliation: Department of Life Sciences, National Central University, Jhongli, Taiwan.
authors.forename: Chien-Chia
authors.initials: CC
authors.lastname: Wang
authors.affiliation: Department of Life Sciences, National Central University, Jhongli, Taiwan.
authors.forename: Ssu Ching
authors.initials: SC
authors.lastname: Chen
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004247:DNA / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects; D004625:Embryo, Nonmammalian; D005659:Fungicides, Industrial / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D007814:Larva / Q000187:drug effects; D012313:RNA / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects*; D014230:Triazoles / Q000633:toxicity*; D015854:Up-Regulation / Q000187:drug effects; D015027:Zebrafish / Q000235:genetics*
chemical_list: D005659:Fungicides, Industrial; D014230:Triazoles; C032910:triadimefon; D012313:RNA; D004247:DNA; D003577:Cytochrome P-450 Enzyme System
keywords: RNA-seq; transcriptome; triadimefon; zebrafish