title: Protective effects of ψ taraxasterol 3-O-myristate and arnidiol 3-O-myristate isolated from Calendula officinalis on epithelial intestinal barrier.
abstract: The triterpene esters ᴪ taraxasterol-3-O-myristate (1) and arnidiol-3-O-myristate (2) were tested for their ability to protect epithelial intestinal barrier in an in vitro model. Their effects on ROS production and on trans-epithelial resistance were investigated on CaCo-2 cell monolayers both in basal and stress-induced conditions. Both compounds were able to modulate the stress damage induced by H2O2 and INFγ+TNFα, showing a potential use as model compounds for the study of new therapeutic agents for intestinal inflammations.
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy. Electronic address: stefano.dallacqua@unipd.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Dall'Acqua
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Catanzaro
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Cocetta
authors.affiliation: NATECO2 GmbH & Co. KG, Auenstrasse 18-20, 85283 Wolnzach, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Igl
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Ragazzi
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy.
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Giron
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Cecconello
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, Padova, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Montopoli
mesh_terms: D018938:Caco-2 Cells; D020838:Calendula / Q000737:chemistry*; D004847:Epithelial Cells / Q000187:drug effects*; D035264:Flowers / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide; D015394:Molecular Structure; D009226:Myristates / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D009226:Myristates; D017382:Reactive Oxygen Species; D014315:Triterpenes; C000608996:arnidiol-3-O-myristate; C000608995:taraxasterol-3-O-myristate; D006861:Hydrogen Peroxide
keywords: CaCo-2 cell monolayers; Calendula officinalis; Intestinal inflammation; ROS; TEER; Triterpene