title: His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase.
abstract: MitoNEET is the first identified iron sulfur protein that located in the mitochondrial outer membrane. We showed that knockdown of mitoNEET did not affect the iron sulfur protein expression in mitochondria and cytoplasm, but significantly reduced the cytosolic aconitase activity. The reduction of aconitase activity was rescued by transfection of wild type mitoNEET, but not by mitoNEET mutants H87C and H87S. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process.
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China. Electronic address: tgq@wmu.edu.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Tan
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Danhui
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Feiyan
authors.initials: F
authors.lastname: Pan
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Tang
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Taizhou Hospital, Wenzhou Medical University, Taizhou 317000, Zhejiang, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China. Electronic address: ljx@wmu.edu.cn.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lyu
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism*; D001665:Binding Sites; D001693:Biological Transport, Active / Q000502:physiology; D003600:Cytosol / Q000378:metabolism*; D056945:Hep G2 Cells; D006639:Histidine / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D061986:MCF-7 Cells; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: C525595:CISD1 protein, human; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins; D006639:Histidine; D000154:Aconitate Hydratase
keywords: Aconitase; His-87 ligand mutations; Iron-sulfur cluster; MitoNEET