title: Does stem cell therapy induce myocardial neoangiogenesis? Histological evaluation in an ischemia/reperfusion animal model.
abstract: BACKGROUND
  In an experimental model in the rabbit, a myocardial ischemia-reperfusion injury was obtained. Subsequently, the effects of homologous bone marrow stem cell (BMSC) administration were studied.
  
  
  METHODS
  In 21 New Zealand adult rabbits, ischemia/reperfusion damage was induced by temporary occlusion of the anterior descending coronary artery. Homologous BMSCs were isolated, cultured and re-suspended for injection at the level of the ischemic zone. We evaluated the proangiogenetic effect of intramyocardial injections of BMSC at the peri-infarcted area. Histological evaluations were made after 20 days from the surgical procedure.
  
  
  RESULTS
  In rabbits treated with intramyocardial BMSC administration, we demonstrated histologically capillary neoangiogenesis, without signs of tissue immunological reaction or of generation of new myocardial cells. On the contrary, only minimal neovascular supply was detected in rabbits treated with intravenous administration of BMSC. Only typical signs of ischemic myocardium injury were observed in the control group.
  
  
  CONCLUSIONS
  These observations suggest that the effect of direct BMSC administration in ischemic myocardium could promote a capillary neoangiogenesis, which helps to prevent ischemic myocardial damage.
authors.affiliation: aDepartment of Life Science bDepartment of Diagnostic Medicine cDepartment of Surgery, University of Modena and RE, Modena, Italy dIstituto Nazionale per le Ricerche Cardiovascolari.
authors.forename: Sonia
authors.initials: S
authors.lastname: Pennella
authors.forename: Luca
authors.initials: L
authors.lastname: Reggiani Bonetti
authors.forename: Mario
authors.initials: M
authors.lastname: Migaldi
authors.forename: Antonio
authors.initials: A
authors.lastname: Manenti
authors.forename: Roberto
authors.initials: R
authors.lastname: Lonardi
authors.forename: Enrico
authors.initials: E
authors.lastname: Giuliani
authors.forename: Alberto
authors.initials: A
authors.lastname: Barbieri
authors.forename: Alberto
authors.initials: A
authors.lastname: Farinetti
authors.forename: Anna V
authors.initials: AV
authors.lastname: Mattioli
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000473:pathology*; D018919:Neovascularization, Physiologic; D011817:Rabbits; D000072657:ST Elevation Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D033581:Stem Cell Transplantation; D013997:Time Factors