title: MiR-670-5p induces cell proliferation in hepatocellular carcinoma by targeting PROX1.
abstract: MiRNAs, as oncogenes or as anti-oncogenes, play critically regulated roles in human cancers at posttranscriptional level. A number of dysregulated miRNAs has been observed in HCC. However, the expression and function of miR-670-5p have not been evaluated in HCC to date. In this study, we examined and confirmed the over-expression of miR-670-5p in HCC and in hepatoma-derived cells Hep3B. At least 60% of HCC tissues showed a greater than three-fold enhance in the expression of miR-670-5p compared with paired adjacent non-cancerous tissues. Knockdown studies for miR-670-5p showed that the expression of miR-670-5p promoted cellular proliferation. In tissues and cells with high expression of miR-670-5p, decreased expression of PROX1, a miR-670-5p predicated target, was detected. It confirmed that PROX1 expression was obviously affected by the expression of miR-670-5p. Furthermore, overexpression of PROX1 greatly inhibitted cellular proliferation. Therefore, it was inferred that miR-670-5p may play important roles in enhancing proliferation activity that is associated with HCC by modulating PROX1 expression at posttranscriptional level.
authors.affiliation: Shandong Jining NO.1 People's Hospital, Jining, PR China.
authors.forename: Cailing
authors.initials: C
authors.lastname: Shi
authors.affiliation: Jining Medical University, Jining, PR China. Electronic address: xuxudong66@aliyun.com.
authors.forename: Xudong
authors.initials: X
authors.lastname: Xu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D016133:Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D000098551:Prospero-Related Homeobox 1 Protein
chemical_list: D018398:Homeodomain Proteins; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; D000098551:Prospero-Related Homeobox 1 Protein
keywords: Hepatocellular carcinoma (HCC); PROX1; Proliferation; miR-670-5p