title: miR-96 inhibits cardiac hypertrophy by targeting growth factor receptor-bound 2.
abstract: Increasing evidence has indicated that microRNAs are involved in the pathogenesis of cardiac hypertrophy. However, whether miR-96 is involved in heart diseases, particularly cardiac hypertrophy, remains unclear. In this study, we found that miR-96 is a negative regulator of cardiac hypertrophy. In primary cardiomyocytes, overexpression of miR-96 inhibited phenylephrine-induced cardiomyocyte hypertrophy and decreased the mRNA expression of cardiac hypertrophy markers such as atrial natriuretic factor and β-myosin heavy chain. Interestingly, we found that growth factor receptor-bound 2 is a direct target of miR-96, which is a negative regulator of cardiac hypertrophy. Overexpression of miR-96 in cardiomyocytes led to reduced growth factor receptor-bound 2 expression. More importantly, miR-96 repressed the extracellular-regulated protein kinase signaling pathway by targeting growth factor receptor-bound 2 in cardiomyocytes. Our data demonstrate that miR-96 is a negative regulator of cardiac hypertrophy and extracellular-regulated protein kinase signaling, thus offering a new therapeutic strategy for cardiac hypertrophy.
authors.affiliation: Department of Cardio-Thoracic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: J
authors.initials: J
authors.lastname: Sheng
authors.affiliation: Department of Cardio-Thoracic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Liang
authors.affiliation: Department of Cardio-Thoracic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: D X
authors.initials: DX
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: A P
authors.initials: AP
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D006332:Cardiomegaly / Q000378:metabolism* / Q000473:pathology; D051380:GRB2 Adaptor Protein / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D035683:MicroRNAs / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D051380:GRB2 Adaptor Protein; C495950:Grb2 protein, rat; C000591374:MIRN96 microRNA, rat; D035683:MicroRNAs