title: Inhibition of herpes virus infection in oligodendrocyte cultured cells by valproic acid.
abstract: Valproic acid (VPA) is a small fatty acid used for treatment of different neurologic diseases such as epilepsy, migraines or bipolar disorders. VPA modulates different processes of cell metabolism that can lead to alterations in susceptibility of several cell types to the infection of Human Immunodeficiency Virus (HIV), Epstein-Barr virus (EBV), as well as to exert an inhibitory effect on the replication of different enveloped viruses in cultured cells. Taken these data into account and the fact that HSV-1 has been involved in some neuropathies, we have characterized the effect of VPA on this herpesvirus infection of the differentiation/maturation-inducible human oligodendrocyte cell line HOG, which resulted more susceptible to VPA inhibition of virus growth after cell differentiation. In these cells, the role of VPA in virus entry was tackled. Incubation with VPA induced a slight but reproducible inhibition in the virus particles uptake mainly observed when the drug was added in the adsorption or early upon infection. In addition, transcription and expression of viral proteins were significantly downregulated in the presence of VPA. Remarkably, when the infective viral production was assessed, VPA dramatically blocked the detection of infectious HSV-1 particles. Herein, our results indicate that VPA treatment of HOG cells significantly reduces the effect of HSV-1 infection, virus entry and productivity without affecting cellular viability.
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049 Madrid, Spain.
authors.forename: A J
authors.initials: AJ
authors.lastname: Crespillo
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049 Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Praena
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049 Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Bello-Morales
authors.affiliation: Universidad Autónoma de Madrid, Facultad de Medicina, Arzobispo Morcillo 4, 28029 Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Lerma
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, 28049 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Vázquez-Calvo
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, 28049 Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Martín-Acebes
authors.affiliation: Universidad Autónoma de Madrid, Facultad de Medicina, Arzobispo Morcillo 4, 28029 Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Tabarés
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, 28049 Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Sobrino
authors.affiliation: Universidad Autónoma de Madrid, Departamento de Biología Molecular, Edificio de Biología, Darwin 2, Cantoblanco, 28049 Madrid, Spain. Electronic address: jal@cbm.csic.es.
authors.forename: J A
authors.initials: JA
authors.lastname: López-Guerrero
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D009836:Oligodendroglia / Q000821:virology*; D014635:Valproic Acid / Q000494:pharmacology*; D053586:Virus Internalization / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D014635:Valproic Acid
keywords: HSV-1; Oligodendrocytes; Valproic acid