title: Phyto-synthesis of silver nanoparticles using Alternanthera tenella leaf extract: an effective inhibitor for the migration of human breast adenocarcinoma (MCF-7) cells.
abstract: In this study, phyto-synthesis of silver nanoparticles (AgNPs) was achieved using an aqueous leaf extract of Alternanthera tenella. The phytochemical screening results revealed that flavonoids are responsible for the AgNPs formation. The AgNPs were characterised using UV-visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray, transmission electron microscopy, fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction. The average size of the nanoparticles was found to be ≈48 nm. The EDX results show that strong signals were observed for the silver atoms. The strong band appearing at 1601-1595 cm(-1) correspond to C-C stretching vibration from dienes in FT-IR spectrum indicating the formation of AgNPs. Human breast adenocarcinoma (MCF-7) cells treated with various concentrations of AgNPs showed a dose-dependent increase in cell inhibition. The IC50 value of the AgNPs was calculated to be 42.5 μg mL(-1). The AgNPs showed a significant reduction in the migration of MCF-7 cells.
authors.affiliation: Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia.
authors.forename: Palanivel
authors.initials: P
authors.lastname: Sathishkumar
authors.affiliation: Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem, 636 011, Tamil Nadu, India.
authors.forename: Krishnan
authors.initials: K
authors.lastname: Vennila
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Rajarajeswaran
authors.initials: R
authors.lastname: Jayakumar
authors.affiliation: Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia. rahim@kimia.fs.utm.my.
authors.forename: Abdull Rahim Mohd
authors.initials: AR
authors.lastname: Yusoff
authors.affiliation: Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia.|Department of Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia.
authors.forename: Tony
authors.initials: T
authors.lastname: Hadibarata
authors.affiliation: Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem, 636 011, Tamil Nadu, India. pal2912@yahoo.com.
authors.forename: Thayumanavan
authors.initials: T
authors.lastname: Palvannan
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D027703:Amaranthaceae / Q000737:chemistry*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D012834:Silver
keywords: Alternanthera tenella; Anticancer activity; Cell migration assay; Flavonoids; Human breast adenocarcinoma cells; Silver nanoparticles