title: Ready display of antigenic peptides in a protein 'mimogen'.
abstract: Given the dependence of much modern biology upon the use of antibodies as tools and reagents, their variability and the often associated lack-of-detail about function and identity creates experimental errors. Here we describe the proof-of-principle for a potentially general, versatile method for the display of antigens in a soluble yet standard format on a lateral protein scaffold that mimics normal epitopes in a protein antigen (a 'mimogen') and confirm their utility in phosphorylation-dependent recognition by specific antibodies.
authors.affiliation: Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. Ben.Davis@chem.ox.ac.uk.
authors.forename: M Robert J
authors.initials: MR
authors.lastname: Vallée
authors.affiliation: Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. Ben.Davis@chem.ox.ac.uk.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Schombs
authors.affiliation: Badrilla Ltd, Leeds Innovation Centre, 103 Clarendon Road, Leeds LS2 9DF, UK and School of Biomedical Science, University of Leeds, Leeds LS2 9JT, UK. J.Colyer@leeds.ac.uk.
authors.forename: Zack J
authors.initials: ZJ
authors.lastname: Balaban
authors.affiliation: Badrilla Ltd, Leeds Innovation Centre, 103 Clarendon Road, Leeds LS2 9DF, UK and School of Biomedical Science, University of Leeds, Leeds LS2 9JT, UK. J.Colyer@leeds.ac.uk.
authors.forename: John
authors.initials: J
authors.lastname: Colyer
authors.affiliation: Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. Ben.Davis@chem.ox.ac.uk.
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Davis
mesh_terms: D000941:Antigens / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*
chemical_list: D000941:Antigens; D010455:Peptides