title: Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae).
abstract: In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Cui
authors.affiliation: Shijiazhuang Post and Telecommunication Technical College, Shijiazhuang 050021, China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Cell and Biochemistry, Institute of Biology, Hebei Academy of Sciences, Shijiazhuang, 050081, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D060829:Arthropod Proteins / Q000737:chemistry* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D026863:Ixodidae / Q000737:chemistry* / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits; D053719:Tandem Mass Spectrometry
chemical_list: D000941:Antigens; D060829:Arthropod Proteins
keywords: Dermacentor silvarum; LC-MS/MS; antigenic protein; tick