title: Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics.
abstract: Molecular changes elicited by plants in response to fungal attack and how this affects plant-pathogen interaction, including susceptibility or resistance, remain elusive. We studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceri (Foc), using quantitative label-free proteomics and NMR-based metabolomics. Results demonstrated differential expression of proteins and metabolites upon Foc inoculations in the resistant plants compared with the susceptible ones. Additionally, expression analysis of candidate genes supported the proteomic and metabolic variations in the chickpea roots upon Foc inoculation. In particular, we found that the resistant plants revealed significant increase in the carbon and nitrogen metabolism; generation of reactive oxygen species (ROS), lignification and phytoalexins. The levels of some of the pathogenesis-related proteins were significantly higher upon Foc inoculation in the resistant plant. Interestingly, results also exhibited the crucial role of altered Yang cycle, which contributed in different methylation reactions and unfolded protein response in the chickpea roots against Foc. Overall, the observed modulations in the metabolic flux as outcome of several orchestrated molecular events are determinant of plant's role in chickpea-Foc interactions.
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Yashwant
authors.initials: Y
authors.lastname: Kumar
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Priyabrata
authors.initials: P
authors.lastname: Panigrahi
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Bhushan B
authors.initials: BB
authors.lastname: Dholakia
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Veena
authors.initials: V
authors.lastname: Dewangan
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Sachin G
authors.initials: SG
authors.lastname: Chavan
authors.affiliation: Central NMR Facility, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Shrikant M
authors.initials: SM
authors.lastname: Kunjir
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Central NMR Facility, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Pattuparambil R
authors.initials: PR
authors.lastname: Rajmohanan
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Narendra Y
authors.initials: NY
authors.lastname: Kadoo
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Ashok P
authors.initials: AP
authors.lastname: Giri
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.|State Key Laboratory of Genetic Engineering, Metabolomics and Systems Biology Laboratory, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Huiru
authors.initials: H
authors.lastname: Tang
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: Vidya S
authors.initials: VS
authors.lastname: Gupta
mesh_terms: D029863:Cicer / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D060467:Disease Resistance; D005670:Fusarium / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000235:genetics; D008031:Lignin / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism; D000094162:Phytoalexins
chemical_list: D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; D008031:Lignin; D000094162:Phytoalexins
keywords: Chickpea; Fusarium oxysporum; NMR; metabolomics; plant-pathogen interaction; proteomics