title: SATB2 expression increased anchorage-independent growth and cell migration in human bronchial epithelial cells.
abstract: The special AT-rich sequence-binding protein 2 (SATB2) is a protein that binds to the nuclear matrix attachment region of the cell and regulates gene expression by altering chromatin structure. In our previous study, we reported that SATB2 gene expression was induced in human bronchial epithelial BEAS-2B cells transformed by arsenic, chromium, nickel and vanadium. In this study, we show that ectopic expression of SATB2 in the normal human bronchial epithelial cell-line BEAS-2B increased anchorage-independent growth and cell migration, meanwhile, shRNA-mediated knockdown of SATB2 significantly decreased anchorage-independent growth in Ni transformed BEAS-2B cells. RNA sequencing analyses of SATB2 regulated genes revealed the enrichment of those involved in cytoskeleton, cell adhesion and cell-movement pathways. Our evidence supports the hypothesis that SATB2 plays an important role in BEAS-2B cell transformation.
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Jordan
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kluz
authors.affiliation: Center for Health Informatics and Bioinformatics, New York University Langone Medical Center, New York, NY 10016, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Cartularo
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA. Electronic address: Max.Costa@nyumc.org.
authors.forename: Max
authors.initials: M
authors.lastname: Costa
mesh_terms: D001980:Bronchi / Q000166:cytology; D002273:Carcinogens / Q000633:toxicity; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D009532:Nickel / Q000633:toxicity; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D002273:Carcinogens; D036961:Matrix Attachment Region Binding Proteins; D034741:RNA, Small Interfering; C521297:SATB2 protein, human; D014157:Transcription Factors; D009532:Nickel
keywords: Cell migration; Metal carcinogenesis; SATB2