title: Population genetic structure of Ascaridia galli of extensively raised chickens of South Africa.
abstract: Ascaridia galli is one of the most common nematode affecting chickens. This study characterized A. galli parasites collected from South African village chickens of Limpopo (n=18) and KwaZulu-Natal (n=22) provinces using the 510bp sequences of cytochrome C oxidase subunit 1 gene of the mitochondrial DNA. Fourteen and 12 polymorphic sites were observed for Limpopo and KwaZulu-Natal sequences, respectively. Six haplotypes were observed in total. Haplotype diversity was high and ranged from 0.749 for Limpopo province to 0.758 for KwaZulu-Natal province isolates. There was no genetic differentiation between A. galli from Limpopo and KwaZulu-Natal provinces. The six South African haplotypes were unique compared to those published in the GeneBank sampled from Hy-line chickens raised under organic farming in Denmark. The utility of cytochrome C oxidase subunit 1 gene as a potential genetic marker for studying A. galli in village chicken populations is presented.
authors.affiliation: Biotechnology Platform, Agricultural Research Council, Onderstepoort, South Africa; Department of Wildlife and Animal Science, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria, South Africa.
authors.forename: D P
authors.initials: DP
authors.lastname: Malatji
authors.affiliation: Parasites, Vectors and Vector-borne Diseases Program, Agricultural Research Council, Onderstepoort, South Africa; Department of Zoology and Entomology, Faculty of Natural Sciences, University of Free State (Qwa-qwa campus), South Africa.
authors.forename: A M
authors.initials: AM
authors.lastname: Tsotetsi
authors.affiliation: Department of Wildlife and Animal Science, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria, South Africa.
authors.forename: E
authors.initials: E
authors.lastname: van Marle-Koster
authors.affiliation: Biotechnology Platform, Agricultural Research Council, Onderstepoort, South Africa. Electronic address: MuchadeyiF@arc.agric.za.
authors.forename: F C
authors.initials: FC
authors.lastname: Muchadeyi
mesh_terms: D000818:Animals; D001197:Ascaridia / Q000235:genetics*; D001198:Ascaridiasis / Q000453:epidemiology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D001483:Base Sequence; D002645:Chickens / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005819:Genetic Markers; D006239:Haplotypes; D007411:Intestinal Diseases, Parasitic / Q000453:epidemiology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D011110:Polymorphism, Genetic; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000517:prevention & control; D015995:Prevalence; D016415:Sequence Alignment / Q000662:veterinary; D013019:South Africa / Q000453:epidemiology
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D005819:Genetic Markers; D003576:Electron Transport Complex IV
keywords: Ascaridia galli; Cytochrome c oxidase subunit 1; Genetic diversity; Village chickens