title: Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.
abstract: Clinically, irreversible pulpitis is treated by the complete removal of pulp tissue followed by replacement with artificial materials. There is considered to be a high potential for autologous transplantation of human dental pulp stem cells (DPSCs) in endodontic treatment. The usefulness of DPSCs isolated from healthy teeth is limited. However, DPSCs isolated from diseased teeth with irreversible pulpitis (IP-DPSCs) are considered to be suitable for dentin/pulp regeneration. In this study, we examined the stem cell potency of IP-DPSCs. In comparison with healthy DPSCs, IP-DPSCs expressed lower colony-forming capacity, population-doubling rate, cell proliferation, multipotency, in vivo dentin regeneration, and immunosuppressive activity, suggesting that intact IP-DPSCs may be inadequate for dentin/pulp regeneration. Therefore, we attempted to improve the impaired in vivo dentin regeneration and in vitro immunosuppressive functions of IP-DPSCs to enable dentin/pulp regeneration. Interferon gamma (IFN-γ) treatment enhanced in vivo dentin regeneration and in vitro T cell suppression of IP-DPSCs, whereas treatment with tumor necrosis factor alpha did not. Therefore, these findings suggest that IFN-γ may be a feasible modulator to improve the functions of impaired IP-DPSCs, suggesting that autologous transplantation of IFN-γ-accelerated IP-DPSCs might be a promising new therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.|Department of Periodontology, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Sonoda
authors.affiliation: Department of Pediatric Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Haruyoshi
authors.initials: H
authors.lastname: Yamaza
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.|Department of Pediatric Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.|Department of Pediatric Dentistry, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.|Department of Pediatric Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Tomoda
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.|Department of Histology and Neuroanatomy, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Reona
authors.initials: R
authors.lastname: Aijima
authors.affiliation: Department of Pediatric Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Nonaka
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kukita
authors.affiliation: Department of Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania, PA, USA.
authors.forename: Songtao
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Periodontology, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Fusanori
authors.initials: F
authors.lastname: Nishimura
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Yamaza
mesh_terms: D000328:Adult; D000818:Animals; D015415:Biomarkers; D049109:Cell Proliferation / Q000187:drug effects; D000066673:Cell Self Renewal; D002470:Cell Survival; D003782:Dental Pulp / Q000166:cytology*; D005260:Female; D006801:Humans; D056747:Immunomodulation; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D010641:Phenotype; D011671:Pulpitis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D007371:Interferon-gamma