title: Overexpression of a citrus basic helix-loop-helix transcription factor (CubHLH1), which is homologous to Arabidopsis activation-tagged bri1 suppressor 1 interacting factor genes, modulates carotenoid metabolism in transgenic tomato.
abstract: To explore the transcription factors associated with carotenoid metabolism in citrus fruit, one transcription factor (CubHLH1) was selected through microarray screening in Satsuma mandarin (Citrus unshiu Marc.) fruit, which was treated with exogenous ethylene or gibberellin (GA), accelerating or retarding carotenoid accumulation in peel, respectively. The amino acid sequence of CubHLH1 has homology to Arabidopsis activation-tagged bri1 suppressor 1 (ATBS1) interacting factor (AIF), which is functionally characterized as a negative regulator of the brassinolide (BR) signalling pathway. Yeast two-hybrid analysis revealed that protein for CubHLH1 could interact with Arabidopsis and tomato ATBS1. Overexpression of CubHLH1 caused a dwarf phenotype in transgenic tomato (Solanum lycopersicum L.), suggesting that CubHLH1 has a similar function to Arabidopsis AIF. In the transgenic tomato fruit at ripening stage, the lycopene content was reduced along with the changes in carotenoid biosynthetic gene expression. The abscisic acid (ABA) content of all the transgenic tomato fruit was higher than that of the wild type. These results implied that CubHLH1 is considered to have a similar function to Arabidopsis AIFs and might be directly involved in carotenoid metabolism in mature citrus fruit.
authors.affiliation: NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio-oriented Research Organization (NARO), Shizuoka 424-0292, Japan. Electronic address: tomoen@affrc.go.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Endo
authors.affiliation: NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio-oriented Research Organization (NARO), Shizuoka 424-0292, Japan. Electronic address: hfujii@affrc.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. Electronic address: sugiyama@dsk.med.kyoto-u.ac.jp.
authors.forename: Aiko
authors.initials: A
authors.lastname: Sugiyama
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. Electronic address: minaka@hiroshima-u.ac.jp.
authors.forename: Michiharu
authors.initials: M
authors.lastname: Nakano
authors.affiliation: NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio-oriented Research Organization (NARO), Shizuoka 424-0292, Japan. Electronic address: nnaoko@affrc.go.jp.
authors.forename: Naoko
authors.initials: N
authors.lastname: Nakajima
authors.affiliation: NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio-oriented Research Organization (NARO), Shizuoka 424-0292, Japan. Electronic address: yoshino@affrc.go.jp.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikoma
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. Electronic address: amomura@ipc.shizuoka.ac.jp.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Omura
authors.affiliation: NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio-oriented Research Organization (NARO), Shizuoka 424-0292, Japan. Electronic address: tshimada@affrc.go.jp.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shimada
mesh_terms: D000595:Amino Acid Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism*; D002957:Citrus / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002338:Carotenoids; D010940:Plant Proteins
keywords: Brassinolide; Carotenoid; Citrus; Light and arabidopsis activation-tagged bri1 suppressor 1 interacting factor (AIF); Transcription factor; bHLH