title: Coexistence of Aflatoxicosis with Protein Malnutrition Worsens Hepatic Oxidative Damage in Rats.
abstract: To investigate the effects of the coexistence of aflatoxin B1 (AFB1) and protein malnutrition in rat liver, weanling rats were fed either normal protein diet (20% protein), low-protein (PEM) diet (5%), normal protein diet + 40 ppb AFB1, or low-protein diet + 40 ppb AFB1. After 8 weeks, biomarkers of hepatic functions and oxidative stress, caspase-3 activity, and tumor suppressor protein 53 (p53) were determined spectrophotometrically. Randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was employed to determine genomic alterations among the groups. Coexistence of aflatoxicosis and PEM significantly decreased glutathione, glutathione-S-transferase, glutathione peroxidase, and superoxide dismutase, while it increased peroxidase and catalase. RAPD-PCR showed genomic alterations that were associated with significant increases in p53 level and caspase-3 activity in rats fed PEM diet + AFB1. In conclusion, the coexistence of aflatoxicosis and protein malnutrition induced oxidative stress with concomitant genomic alterations in the liver of weanling rats.
authors.affiliation: Department of Biological Sciences, Covenant University, Ota, Nigeria.
authors.forename: Oluwakemi A
authors.initials: OA
authors.lastname: Rotimi
authors.affiliation: Department of Biological Sciences, Covenant University, Ota, Nigeria.
authors.forename: Solomon O
authors.initials: SO
authors.lastname: Rotimi
authors.affiliation: Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria.
authors.forename: Flora
authors.initials: F
authors.lastname: Oluwafemi
authors.affiliation: Department of Biochemistry, Federal University of Agriculture, Abeokuta, Nigeria.
authors.forename: Oladipo
authors.initials: O
authors.lastname: Ademuyiwa
authors.affiliation: Department of Biochemistry, University of Ilorin, Ilorin, Nigeria. ea_balogun@yahoo.com.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Balogun
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D031204:Caloric Restriction / Q000009:adverse effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D005260:Female; D005786:Gene Expression Regulation; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53; D016604:Aflatoxin B1; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; C505410:Casp3 protein, rat; D053148:Caspase 3; D005978:Glutathione
keywords: Aflatoxin B1; DNA; Liver; Oxidative Stress; Protein Malnutrition