title: Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages.
abstract: The aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation.
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Ozaydin
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
authors.forename: Emrah
authors.initials: E
authors.lastname: Sur
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Oznurlu
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
authors.forename: Ilhami
authors.initials: I
authors.lastname: Celik
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, University of Selcuk, TR-42075, Campus, Konya, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Uluisik
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D005260:Female; D005865:Gestational Age; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice / Q000378:metabolism; D010920:Placenta / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000378:metabolism*; D014327:Trophoblasts / Q000737:chemistry / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018809:Proliferating Cell Nuclear Antigen
keywords: Hsp70; PCNA; decidualization; mouse; placentation