title: Detection of dengue NS1 and NS3 proteins in placenta and umbilical cord in fetal and maternal death.
abstract: In Brazil, dengue is a public health problem with the occurrence of explosive epidemics. This study reports maternal and fetal deaths due to dengue and which tissues of placenta and umbilical cord were analyzed by molecular methods and immunohistochemistry. The dengue NS3 and NS1 detection revealed the viral presence in different cells from placenta and umbilical cord. In the latter, DENV-2 was detected at a viral titer of 1,02 × 10(4) amounts of viral RNA. It was shown that the DENV markers analyzed here may be an alternative approach for dengue fatal cases investigation, especially involving maternal and fetal death. J. Med. Virol. 88:1448-1452, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratório de Flavivirus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Priscila Conrado Guerra
authors.initials: PC
authors.lastname: Nunes
authors.affiliation: Laboratório de Biotecnologia e Fisiologia de Infecções Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Marciano Viana
authors.initials: MV
authors.lastname: Paes
authors.affiliation: Hospital Universitário Gaffrée Guinle, Departamento de Anatomia Patológica, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Carlos Alberto Basilio
authors.initials: CA
authors.lastname: de Oliveira
authors.affiliation: Subsecretaria de Atenção Primária, Vigilância e Promoção da Saúde, Secretaria Municipal de Saúde do Rio de Janeiro-RJ, Brazil.
authors.forename: Ana Carla Gomes
authors.initials: AC
authors.lastname: Soares
authors.affiliation: Laboratório de Flavivirus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Ana Maria Bispo
authors.initials: AM
authors.lastname: de Filippis
authors.affiliation: Laboratório de Flavivirus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Monique da Rocha Queiroz
authors.initials: Mda R
authors.lastname: Lima
authors.affiliation: Laboratório de Biotecnologia e Fisiologia de Infecções Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Ada Maria
authors.initials: AM
authors.lastname: de Barcelos Alves
authors.affiliation: Laboratório de Biotecnologia e Fisiologia de Infecções Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Juliana Fernandes Amorim
authors.initials: JF
authors.lastname: da Silva
authors.affiliation: Laboratório de Anatomia Patológica, Instituto Nacional de Infectologia, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Janice Mery Chicarino
authors.initials: JM
authors.lastname: de Oliveira Coelho
authors.affiliation: Laboratório de Anatomia Patológica, Instituto Nacional de Infectologia, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Francisco das Chagas
authors.initials: Fd
authors.lastname: de Carvalho Rodrigues
authors.affiliation: Laboratório de Flavivirus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Rita Maria Ribeiro
authors.initials: RM
authors.lastname: Nogueira
authors.affiliation: Laboratório de Flavivirus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz /FIOCRUZ, Rio de Janeiro- RJ, Brazil.
authors.forename: Flávia Barreto
authors.initials: FB
authors.lastname: Dos Santos
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005313:Fetal Death / Q000209:etiology*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000821:virology; D063130:Maternal Death / Q000209:etiology*; D010920:Placenta / Q000166:cytology / Q000473:pathology / Q000821:virology*; D011247:Pregnancy; D020365:RNA Helicases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012697:Serine Endopeptidases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012698:Serologic Tests; D014470:Umbilical Cord / Q000166:cytology / Q000473:pathology / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D055815:Young Adult; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D020365:RNA Helicases; D012367:RNA, Viral; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C079340:NS1 protein, Dengue virus type 2; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: Brazil; NS1; NS3; dengue; maternal and fetal death