title: Species composition, co-occurrence, association and affinity indices of mosquito larvae (Diptera: Culicidae) in Mazandaran Province, northern Iran.
abstract: Although considerable progress has been made in the past years in management of mosquito borne diseases such as malaria, dengue, yellow fever and West Nile fever through research in biology and ecology of the vectors, these diseases are still major threats to human health. Therefore, more research is required for better management of the diseases. This investigation provides information on the composition, co-occurrence, association and affinity indices of mosquito larvae in Mazandaran Province, northern Iran. In a large scale field study, mosquito larvae were collected from 120 sentinel sites in 16 counties in Mazandaran Province, using standard 350 ml dipper. Sampling took place monthly from May to December 2014. Collected larvae were mounted on glass slides using de Faure's medium and were diagnosed using morphological characters. Totally, 19,840 larvae were collected including three genera and 16 species from 120 larval habitats, as follows: Anopheles claviger, Anopheles hyrcanus, Anopheles maculipennis s.l., Anopheles marteri, Anopheles plumbeus, Anopheles pseudopictus, Culex pipiens, Culex tritaeniorhynchus, Culex torrentium, Culex perexiguus, Culex territans, Culex mimeticus, Culex hortensis, Culiseta annulata, Culiseta longiareolata, and Culiseta morsitans. Predominant species were Cx. pipiens and An. maculipennis s.l. which show the highest co-occurrence. The pair of species An. hyrcanus/An. pseudopictus showed significant affinity and association. High co-occurrence of the predominant species Cx. pipiens and An. maculipennis s.l. in the study area is of considerable importance in terms of vector ecology. It was also revealed that An. pseudopictus/An. hyrcanus often occur sympatrically indicating their common habitat requirements. The information may be equally important when vector control measures are considered.
authors.affiliation: Department of Medical Entomology and Vector Control, School of Public Health and Health Sciences Research Center, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Seyed Hassan
authors.initials: SH
authors.lastname: Nikookar
authors.affiliation: School of Health, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Shahyad
authors.initials: S
authors.lastname: Azari-Hamidian
authors.affiliation: Department of Medical Entomology and Vector Control, School of Public Health and Health Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Fazeli-Dinan
authors.affiliation: Department of Biostatistics, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Seyed Nouraddin Mousavi
authors.initials: SN
authors.lastname: Nasab
authors.affiliation: Department of Epidemiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Aarabi
authors.affiliation: Department of Medical Entomology and Vector Control, School of Public Health and Health Sciences Research Center, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran; Department of Parasitology, North Research Center, Pasteur Institute of Iran, Amol, Iran.
authors.forename: Seyyed Payman
authors.initials: SP
authors.lastname: Ziapour
authors.affiliation: Department of Medical Entomology and Vector Control, School of Public Health and Health Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address: ahmadali_enayati@yahoo.com.
authors.forename: Ahmadali
authors.initials: A
authors.lastname: Enayati
mesh_terms: D000818:Animals; D000852:Anopheles; D044822:Biodiversity; D003465:Culex; D003715:Dengue / Q000453:epidemiology / Q000469:parasitology*; D004199:Disease Vectors; D004463:Ecology; D017753:Ecosystem; D006801:Humans; D007492:Iran / Q000453:epidemiology; D007814:Larva; D008288:Malaria / Q000453:epidemiology / Q000469:parasitology*; D014901:West Nile Fever / Q000453:epidemiology; D015004:Yellow Fever / Q000453:epidemiology / Q000469:parasitology*
keywords: Affinity index; Anopheles; Association; Co-occurrence; Culex; Culiseta; Larval habitat