title: Ultrasensitive In Situ Fluorescence Analysis using Modulated Fluorescence Interference Contrast at Nanostructured Polymer Surfaces.
abstract: The precise modulation of fluorescence interference contrast is achieved by introducing a nanoscopically engineered spacer layer prepared by chemical vapor deposition (CVD) of functional polymers. These novel imaging substrates are chemically identical throughout their entire detection area, yet present patterns of nanoscale thickness. A protein binding cascade is studied in real time and in the presence of high background noise.
authors.affiliation: Biointerfaces Institute & Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Deng
authors.affiliation: Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
authors.forename: Siheng
authors.initials: S
authors.lastname: He
authors.affiliation: Biointerfaces Institute & Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
authors.forename: Fan
authors.initials: F
authors.lastname: Xie
authors.affiliation: Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Friedmann
authors.affiliation: Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
authors.forename: Henry
authors.initials: H
authors.lastname: Hess
authors.affiliation: Biointerfaces Institute & Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.|Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Lahann
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D019793:Fluorescein / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
chemical_list: D011108:Polymers; D012710:Serum Albumin, Bovine; D006046:Gold; D019793:Fluorescein
keywords: CVD; fluorescence interference contrast; lock-in analysis; nanostructured surfaces; protein adsorption; reactive polymers