title: Serum Metabolomic Profiling in a Rat Model Reveals Protective Function of Paeoniflorin Against ANIT Induced Cholestasis.
abstract: Cholestasis is a leading cause of hepatic accumulation of bile acids resulting in liver injury, fibrosis, and liver failure. Paeoniflorin displays bright prospects in liver protective effect. However, its molecular mechanism has not been well-explored. This study was designed to assess the effects and possible mechanisms of paeoniflorin against alpha-naphthylisothiocyanate-induced liver injury. Ultraperformance liquid chromatography coupled with quadrupole time-of-flight combined with principle component analysis and partial least squares discriminant analysis were integrated to obtain differentiating metabolites for the pathways and clarify mechanisms of disease. The results indicated that paeoniflorin could remarkably downregulate serum biochemical indexes and alleviate the histological damage of liver tissue. Different expression of 14 metabolites demonstrated that paeoniflorin mainly regulated the dysfunctions of glycerophospholipid metabolism and primary bile acid biosynthesis. Moreover, several pathways such as arginine and proline metabolism, ether lipid metabolism, and arachidonic acid metabolism were also related to the efficacy. In conclusion, paeoniflorin has indicated favorable pharmacological effect on serum biochemical indexes and pathological observation on cholestatic model. And metabolomics is a promising approach to unraveling hepatoprotective effects by partially regulating the perturbed pathways, which provide insights into mechanisms of cholestasis.
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.|College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.|College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Niu
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Jiabo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Liver Failure Treatment and Research Center, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Ruilin
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Zhenman
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Hongge
authors.initials: H
authors.lastname: Chen
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army, Beijing, 100039, China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
mesh_terms: D015058:1-Naphthylisothiocyanate / Q000009:adverse effects; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D002779:Cholestasis / Q000139:chemically induced / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D055442:Metabolome; D039821:Monoterpenes / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D006027:Glycosides
chemical_list: D015058:1-Naphthylisothiocyanate; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides
keywords: cholestasis; mechanism; metabolomics; paeoniflorin; pattern recognition approaches