title: Correlation of Cyfra 21-1 levels in saliva and serum with CK19 mRNA expression in oral squamous cell carcinoma.
abstract: Oral squamous cell carcinoma (OSCC) accounts for 90 % of malignant lesions of oral cavity. The study assessed the potential of Cyfra 21-1 as a tumor marker in OSCC. The study included 50 patients of OSCC to evaluate levels of Cyfra 21-1 in serum and saliva by electrochemiluminescent immunoassay (ECLIA) and CK19 messenger RNA (mRNA) expression in tissue by florescent quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) along with healthy individuals as control. The salivary and serum Cyfra 21-1 levels in patients of OSCC were significantly higher compared to controls (p value < 0.01). There was a 2.75-fold increase in CK19 mRNA expression in OSCC cases compared to controls. A significant positive correlation was found between serum and salivary Cyfra 21-1, serum Cyfra 21-1, and CK19 mRNA expression and between salivary Cyfra 21-1 and CK19 mRNA expression. Among these, correlation between serum and salivary Cyfra 21-1 was highly significant. Salivary and serum Cyfra 21-1 showed significantly elevated levels in grade II OSCC compared to grade I histopathologically. Elevated levels of salivary Cyfra 21-1 were associated with recurrence in OSCC patients. Reverse operating curve constructed using 3 ng/ml as a cutoff for serum Cyfra 21-1 revealed the sensitivity and specificity to be 88 and 78.2 %, respectively. Using a cutoff value of 8.5 ng/ml for salivary Cyfra 21-1, the sensitivity was found to be 93.8 % and specificity 84.3 %. We advocate salivary Cyfra 21-1 as a better diagnostic marker over serum Cyfra 21-1 as well as a potential marker in the prognosis of OSCC.
authors.affiliation: Department of Oral Pathology and Microbiology, Maulana Azad Institute of Dental Sciences, Bahadur Shah Zafar Marg, New Delhi, 110002, India.
authors.forename: Rewa
authors.initials: R
authors.lastname: Malhotra
authors.affiliation: Department of Oral Pathology and Microbiology, Maulana Azad Institute of Dental Sciences, Bahadur Shah Zafar Marg, New Delhi, 110002, India. draadithyaburs@gmail.com.
authors.forename: Aadithya B
authors.initials: AB
authors.lastname: Urs
authors.affiliation: Department of Microbiology, Maulana Azad Medical College, New Delhi, India.
authors.forename: Anita
authors.initials: A
authors.lastname: Chakravarti
authors.affiliation: Department of Microbiology, Maulana Azad Medical College, New Delhi, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, G. B. Pant Hospital, New Delhi, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Department of Biochemistry, G. B. Pant Hospital, New Delhi, India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Mahajan
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood* / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000378:metabolism*; D006801:Humans; D053539:Keratin-19 / Q000097:blood / Q000378:metabolism*; D009055:Mouth / Q000378:metabolism; D009062:Mouth Neoplasms / Q000097:blood / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local / Q000097:blood / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D053539:Keratin-19; D012333:RNA, Messenger; C102012:antigen CYFRA21.1
keywords: Cyfra 21-1; Cytokeratin19; Electrochemiluminescent immunoassay; Oral squamous cell carcinoma; Polymerase chain reaction