title: Nanocomplexes from RGD-modified generation 1.0 polyamidoamine based copolymers used for intravascular gene release to prevent restenosis.
abstract: OBJECTIVE
  To validate the efficacy of nanocomplexes from RGD-modified polyamidoamine (PAMAM G1) copolymer for prevention of restenosis.
  
  
  METHODS
  Generation 1.0 polyamidoamine (PAMAM G1)-based copolymers (PGP) and RGD modified PGP (PGP-RGD) were synthesized and its properties were evaluated. Intravascular VEGF165 release tests were performed.
  
  
  RESULTS
  The PGP-RGD1 (2.6% grafting rate) exhibited lower cytotoxicity and larger combining ability with pDNA. The complexes had sizes of 80-160 nm and zeta potentials of 3-20 mV. Transfection efficiency of PGP-RGD1 complexes in human umbilical vein endothelial cells was larger than that of PGP complexes. Patency and expression level of artery in PGP-RGD1 group were higher than that in saline group.
  
  
  CONCLUSIONS
  PGP-RGD1 will be a promising targeted gene vector.
authors.affiliation: The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2 Road, Guangzhou 510080, P. R. China.|Department of Pharmaceutical Science, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue, Guangzhou 510515, P. R. China.
authors.forename: Duanwen
authors.initials: D
authors.lastname: Cao
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, P. R. China.
authors.forename: Shouqin
authors.initials: S
authors.lastname: Tian
authors.affiliation: The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2 Road, Guangzhou 510080, P. R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Yi
authors.affiliation: The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2 Road, Guangzhou 510080, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Dai
authors.affiliation: Department of Pharmaceutical Science, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue, Guangzhou 510515, P. R. China.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2 Road, Guangzhou 510080, P. R. China.
authors.forename: Shirong
authors.initials: S
authors.lastname: Pan
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000378:metabolism*; D003251:Constriction, Pathologic / Q000517:prevention & control*; D015316:Genetic Therapy / Q000379:methods*; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053758:Nanoparticles; D009842:Oligopeptides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011817:Rabbits; D012008:Recurrence; D014162:Transfection
chemical_list: D009842:Oligopeptides; C531249:Poly(amidoamine); D011073:Polyamines; C047981:arginyl-glycyl-aspartic acid
keywords: PAMAM G1; RGD peptide; human umbilical vein endothelial cells; intravascular specific site release; nanocomplexes; pVEGF165 gene; patency; restenosis; targeting; transfection efficiency