title: Analysis of Isoquinoline Alkaloid Composition and Wound-Induced Variation in Nelumbo Using HPLC-MS/MS.
abstract: Alkaloids are the most relevant bioactive components in lotus, a traditional herb in Asia, but little is known about their qualitative and quantitative distributions. Here, we report on the alkaloid composition in various lotus organs. Lotus laminae and embryos are rich in isoquinoline alkaloids, whereas petioles and rhizomes contain trace amounts of alkaloids. Wide variation of alkaloid accumulation in lamina and embryo was observed among screened genotypes. In laminae, alkaloid accumulation increases during early developmental stages, reaches the highest level at full size stage, and then decreases slightly during senescence. Vegetative and embryogenic tissues accumulate mainly aporphine-type and bisbenzylisoquinoline-type alkaloids, respectively. Bisbenzylisoquinoline-type alkaloids may be synthesized mainly in lamina and then transported into embryo via latex through phloem translocation. In addition, mechanical wounding was shown to induce significant accumulation of specific alkaloids in lotus leaves.
authors.affiliation: Sino-African Joint Research Center, Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Xianbao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Graduate University of Chinese Academy of Sciences , 19A Yuquanlu, Beijing 100049, People's Republic of China.
authors.forename: Lingping
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Graduate University of Chinese Academy of Sciences , 19A Yuquanlu, Beijing 100049, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fang
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok 10330, Thailand.
authors.forename: Sornkanok
authors.initials: S
authors.lastname: Vimolmangkang
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: Graduate University of Chinese Academy of Sciences , 19A Yuquanlu, Beijing 100049, People's Republic of China.
authors.forename: Collins
authors.initials: C
authors.lastname: Ogutu
authors.forename: Yanling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Sino-African Joint Research Center, Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Han
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007546:Isoquinolines / Q000737:chemistry* / Q000378:metabolism; D031653:Nelumbo / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000470:Alkaloids; D007546:Isoquinolines; D010936:Plant Extracts; C039109:isoquinoline
keywords: Nelumbo; alkaloids; bioactive components; mechanical wounding