title: Occurrence, Virulence Factors, Antimicrobial Resistance, and Genotyping of Staphylococcus aureus Strains Isolated from Chicken Products and Humans.
abstract: Staphylococcus aureus in food is a consequence of inadequate hygienic handling and processing, posing a potential risk to public health. The current study aimed to characterize virulence factors, as well as antimicrobial resistance of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) isolated from retail chicken products and hand swabs from vendors in Egypt. In addition, genetic relatedness of the isolates from chicken and humans was evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using protein A as a target. A total of 110 samples were collected from chicken products (n = 80) and vendors (n = 30). Overall, 30 (37.5%) chicken products samples were positive for S. aureus, whereas hand swabs from meat handlers revealed that 18 (60%) were positive. Ten MRSA strains were characterized by the presence of the mecA gene, comprising seven isolates from chicken and three from humans. Virulence-associated factors were evaluated by PCR, revealing that 31.3% of S. aureus isolates harbored the Panton-Valentine leukocidin (PVL) gene, whereas 10.4% were positive for the sea and sed genes each, and only two isolates were positive for γ-hemolysin-associated gene. Genotyping using spa PCR-RFLP showed identical restriction banding patterns of MRSA isolates of human and chicken meat origin, indicating the genetic relatedness of the isolates. To the best of our knowledge, this is the first study to characterize PVL-positive MRSA from chicken products and to utilize spa-RFLP for evaluating the genetic relatedness between MRSA of human and chicken origin in Egypt.
authors.affiliation: 1 Department of Food Control, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .
authors.forename: Rasha M
authors.initials: RM
authors.lastname: El Bayomi
authors.affiliation: 2 Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .
authors.forename: Heba A
authors.initials: HA
authors.lastname: Ahmed
authors.affiliation: 2 Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .
authors.forename: Maysa A I
authors.initials: MA
authors.lastname: Awadallah
authors.affiliation: 3 Department of Microbiology, Animal Health Research Institute , Mansoura Branch, Mansoura, Egypt .
authors.forename: Rasha A
authors.initials: RA
authors.lastname: Mohsen
authors.affiliation: 3 Department of Microbiology, Animal Health Research Institute , Mansoura Branch, Mansoura, Egypt .
authors.forename: Abeer E
authors.initials: AE
authors.lastname: Abd El-Ghafar
authors.affiliation: 3 Department of Microbiology, Animal Health Research Institute , Mansoura Branch, Mansoura, Egypt .
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Abdelrahman
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002645:Chickens / Q000382:microbiology*; D004534:Egypt / Q000453:epidemiology; D005098:Exotoxins / Q000235:genetics; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D007956:Leukocidins / Q000235:genetics; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D046915:Penicillin-Binding Proteins / Q000235:genetics; D011200:Poultry / Q000382:microbiology; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; D013205:Staphylococcal Protein A; D037521:Virulence Factors; C487789:mecA protein, Staphylococcus aureus; D008712:Methicillin
keywords: Enterotoxins; MRSA; PVL; S. aureus; spa-PCR-RFLP; γ-Hemolysin