title: [Effect of DPP4 inhibitor sitagliptin on expressions of early growth response-1 and fibronectin in the kidney of ApoE gene knockout mice].
abstract: OBJECTIVE
  To investigate the effects of the DPP4 inhibitor sitagliptin on the expressions of early growth response-1 (Egr-1) and fibronectin in the kidney of ApoE gene knockout mice.
  
  
  METHODS
  Eight-week-old male ApoE gene knockout mice were randomly divided into sitagliptin + apoE(-/-) group and apoE(-/-) group (n=6), with 6 C57BL mice as the normal control group. After feeding with high-fat diet and drug treatment for 16 weeks, the mice underwent intraperitoneal glucose tolerance test (IPGTT) and were measured for 24-h urinary albumin using ELISA. All the mice were then sacrificed to examine the changes of blood lipid profile and for detection of Egr-1 and fibronectin mRNA and proteins in the renal tissue using real-time PCR and Western blotting.
  
  
  RESULTS
  The mice in both apoE(-/-) group and sitagliptin+apoE(-/-) group all showed prominently increased blood lipids as compared with the control group (P<0.05) without significant differences between the two apoE(-/-) groups. The level of HDL was significantly higher in sitagliptin +apoE(-/-) group than in apoE(-/-) group (P<0.001) and control group (P<0.001). IPGTT showed no significant differences in the levels of blood glucose among the 3 groups. The excretion of urinary albumin was increased in apoE(-/-) group compared with the control group (P<0.01), but was significantly lower in sitagliptin+ apoE(-/-) group than in apoE(-/-) group (P<0.01). Real-time PCR and Western blotting showed significantly decreased mRNA and protein expressions of renal cortical Egr-1 and fibronectin in sitagliptin+apoE(-/-) group compared with apoE(-/-) group.
  
  
  CONCLUSIONS
  Sitagliptin can reduce the renal expression of fibronectin by regulating the expression of Egr-1 to achieve renal protection.
authors.affiliation: Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: wslwq01@163.com.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Li
authors.forename: Meiping
authors.initials: M
authors.lastname: Guan
authors.forename: Zongji
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D059305:Diet, High-Fat; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000494:pharmacology*; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D055786:Gene Knockout Techniques; D007668:Kidney / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D060888:Real-Time Polymerase Chain Reaction; D000068900:Sitagliptin Phosphate / Q000494:pharmacology*
chemical_list: D001057:Apolipoproteins E; D054873:Dipeptidyl-Peptidase IV Inhibitors; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D005353:Fibronectins; D008055:Lipids; D000068900:Sitagliptin Phosphate