title: Characterization of Soybean Storage and Allergen Proteins Affected by Environmental and Genetic Factors.
abstract: There is limited information on the influence of genetic and environmental variability on soybean protein composition. This study aimed to determine the role of genotype (G), environments (E), and the interrelationship of genotype and environment (G×E) on soybean seed protein. Three sets of nine soybean genotypes were grown in replicated trials at Maryland, South Carolina, and South Dakota. At each location, the nine genotypes were grown with two planting/sowing dates. We applied two-dimensional gel electrophoresis and mass spectrometry to study the variability of soybean storage and allergen proteins. Statistical analysis of 47 storage and 8 allergen proteins, in terms of differentially expressed protein spots significant at the p<0.005 level, was performed. We found more spots that showed statistically significant differences in expression among E compared to G and G×E interaction.
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARS , Beltsville, Maryland 20705, United States.
authors.forename: Savithiry
authors.initials: S
authors.lastname: Natarajan
authors.affiliation: Department of Plant Science & Landscape Architecture, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: Farooq
authors.initials: F
authors.lastname: Khan
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARS , Beltsville, Maryland 20705, United States.
authors.forename: Qijian
authors.initials: Q
authors.lastname: Song
authors.affiliation: Diet, Genomics and Immunology Laboratory, USDA-ARS , Beltsville, Maryland 20705, United States.
authors.forename: Sukla
authors.initials: S
authors.lastname: Lakshman
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARS , Beltsville, Maryland 20705, United States.
authors.forename: Perry
authors.initials: P
authors.lastname: Cregan
authors.affiliation: Crop Production and Protection, Oilseeds & Bioscience, USDA-ARS , Beltsville, Maryland 20705, United States.
authors.forename: Roy
authors.initials: R
authors.lastname: Scott
authors.affiliation: Clemson University , Department of Entomology, Soils, & Plant Sciences, Clemson, South Carolina 29634, United States.
authors.forename: Emerson
authors.initials: E
authors.lastname: Shipe
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, USDA-ARS , Beltsville, Maryland 20705, United States.
authors.forename: Wesley
authors.initials: W
authors.lastname: Garrett
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D040901:Proteomics; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D030262:Soybean Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013025:Glycine max / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
chemical_list: D030262:Soybean Proteins
keywords: 2D-PAGE; G. max; G. soja; LC-MS; MALDI-TOF-MS; allergen; environment; genotype; glycinin; location; soybean; β-conglycinin