title: Backbone Assignment of the MALT1 Paracaspase by Solution NMR.
abstract: Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is a unique paracaspase protein whose protease activity mediates oncogenic NF-κB signalling in activated B cell-like diffuse large B cell lymphomas (ABC-DLBCLs). ABC-DLBCLs are aggressive lymphomas with high resistance to current chemotherapies. Low survival rate among patients emphasizes the urgent need for alternative treatment options. The characterization of the MALT1 will be an essential tool for developing new target-directed drugs against MALT1 dependent disorders. As the first step in the atomic-level NMR studies of the system, here we report, the (15)N/(13)C/(1)H backbone assignment of the apo form of the MALT1 paracaspase region together with the third immunoglobulin-like (Ig3) domain, 44 kDa, by high resolution NMR. In addition, the non-uniform sampling (NUS) based targeted acquisition procedure is evaluated as a mean of decreasing acquisition and analysis time for larger proteins.
authors.affiliation: Medivir AB, PO Box 1086, SE-141 22, Huddinge, Sweden.
authors.forename: Sofia
authors.initials: S
authors.lastname: Unnerståle
authors.affiliation: Swedish NMR Centre, University of Gothenburg, PO Box 465, SE-40530, Gothenburg, Sweden.|Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097, Warsaw, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Nowakowski
authors.affiliation: Medivir AB, PO Box 1086, SE-141 22, Huddinge, Sweden.
authors.forename: Vera
authors.initials: V
authors.lastname: Baraznenok
authors.affiliation: Medivir AB, PO Box 1086, SE-141 22, Huddinge, Sweden.
authors.forename: Gun
authors.initials: G
authors.lastname: Stenberg
authors.affiliation: Medivir AB, PO Box 1086, SE-141 22, Huddinge, Sweden.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Lindberg
authors.affiliation: Swedish NMR Centre, University of Gothenburg, PO Box 465, SE-40530, Gothenburg, Sweden.
authors.forename: Maxim
authors.initials: M
authors.lastname: Mayzel
authors.affiliation: Swedish NMR Centre, University of Gothenburg, PO Box 465, SE-40530, Gothenburg, Sweden.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Orekhov
authors.affiliation: Medivir AB, PO Box 1086, SE-141 22, Huddinge, Sweden.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Agback
mesh_terms: D020169:Caspases / Q000737:chemistry*; D006801:Humans; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein; D009363:Neoplasm Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D009363:Neoplasm Proteins; D020169:Caspases; C400109:MALT1 protein, human; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein