title: Correction of Murine Diabetic Hyperglycaemia With A Single Systemic Administration of An AAV2/8 Vector Containing A Novel Codon Optimized Human Insulin Gene.
abstract: We report the correction of hyperglycemia of STZ induced diabetic mice using one intravenous systemic administration of a single stranded serotype 8 pseudotyped adeno-associated virus (ssAAV2/8) vector encoding the human proinsulin gene under a constitutive liver specific promoter. In vivo dose titration experiments were carried out and we identified an optimal range that achieved maintenance of euglycaemia or a mild diabetic condition for at least 9 months and ongoing to beyond 1 year for some animals, accompanied by human C-peptide secretion and weight gain. Further DNA codon optimization of the insulin gene construct resulted in approximately 3-10 times more human C-peptide secreted in the blood of codon optimized treated animals thereby reducing the number of vector particles required to achieve the same extent of reduction in blood glucose levels as the non-codon optimized vector. The constitutive secretion of insulin achieved with a single administration of the vector could be of therapeutic value for some diabetic patients.
authors.forename: Shu Uin
authors.initials: SU
authors.lastname: Gan
authors.forename: Maria
authors.initials: M
authors.lastname: Notaridou
authors.forename: Zhen Ying
authors.initials: ZY
authors.lastname: Fu
authors.forename: Kok Onn
authors.initials: KO
authors.lastname: Lee
authors.forename: Kian Chuan
authors.initials: KC
authors.lastname: Sia
authors.forename: Amit Chunilal
authors.initials: AC
authors.lastname: Nathwani
authors.forename: Marco
authors.initials: M
authors.lastname: Della Peruta
authors.affiliation: Department of Surgery, University of Cambridge, Cambridge, UK. ryc1000@medschl.cam.ac.uk.
authors.forename: Roy Yorke
authors.initials: RY
authors.lastname: Calne
mesh_terms: D000818:Animals; D002096:C-Peptide / Q000378:metabolism; D003062:Codon; D000229:Dependovirus / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000628:therapy; D005822:Genetic Vectors / Q000008:administration & dosage*; D006801:Humans; D006943:Hyperglycemia / Q000235:genetics / Q000628:therapy*; D007328:Insulin / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics
chemical_list: D002096:C-Peptide; D003062:Codon; D007328:Insulin; D011994:Recombinant Proteins