title: Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma.
abstract: Ki67 can discriminate between high- and low-grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67-positive cells), evaluated by flow cytometry, in 40 dogs with high-grade B-cell lymphoma, treated with a modified Wisconsin-Madison protocol (UW-25). The following variables were investigated for association with lymphoma specific survival (LSS) and relapse free interval (RFI): Ki67%, breed, sex, age, stage, substage, complete remission (CR). By multivariate analysis, Ki67% (P = 0.009) and achievement of CR (P = 0.001) were independent prognostic factors for LSS. Dogs with intermediate Ki67% (20.1-40%) presented longer LSS and RFI (median = 866 and 428 days, respectively) than dogs with low (median = 42 days, P < 0.001; median = 159 days, P = 0.014) or high (median = 173 days, P = 0.038; median = 100 days, P = 0.126) values. Determination of Ki67 is a prognostic tool that improves the clinical usefulness of flow cytometric analysis in canine high-grade B-cell lymphoma.
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Poggi
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Miniscalco
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Morello
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gattino
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Delaude
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Ferrero Poschetto
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Legnaro, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Aresu
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Legnaro, Italy.
authors.forename: M E
authors.initials: ME
authors.lastname: Gelain
authors.affiliation: Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
authors.forename: V
authors.identifier: http://orcid.org/0000-0002-9882-2867
authors.initials: V
authors.lastname: Martini
authors.affiliation: Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Comazzi
authors.affiliation: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Riondato
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy / Q000401:mortality; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D019394:Ki-67 Antigen / Q000097:blood*; D016393:Lymphoma, B-Cell / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary*; D008297:Male; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D019394:Ki-67 Antigen
keywords: Ki67; dog; flow cytometry; lymphoma; prognosis