title: Pyruvate alleviates lipid peroxidation and multiple-organ dysfunction in rats with hemorrhagic shock.
abstract: OBJECTIVE
  Pyruvate can reduce lipid peroxidation, which plays a critical role in organ injury, in various models. However, it is not fully understood if this inhibition occurs in resuscitation of hemorrhagic shock (HS). This study examines effects of pyruvate Ringer solution (PR) in this respect in rats.
  
  
  METHODS
  Rats, subjected to 45% blood loss, were randomly allocated to the 3 groups (n = 18): HS with no fluid resuscitation (group NR), HS resuscitated with lactated Ringer solution (LR) (group LR), and HS resuscitated with PR (group PR). Mean arterial pressure, plasma levels of thiobarbituric acid reactive substances (TBARS), and superoxide dismutase were measured at various time points until 360 minutes after hemorrhage. Visceral organs were harvested at the end for evaluations of the TBARS, antioxidant enzyme, and tissue water content. Other 54 rats with identical procedures without sampling were documented for 24-hour survival rates (n = 18) after fluid resuscitation.
  
  
  RESULTS
  Pyruvate Ringer solution significantly increased mean arterial pressure and decreased blood TBARS levels after lethal HS. It also reduced TBARS concentrations and glutathione peroxidase activities but significantly enhanced glutathione reductase activities in most organs and greatly improved the ratios of reduced glutathione over oxidized glutathione in various organs in group PR, compared to group LR. Furthermore, PR significantly improved various organ function and water contents relative to LR. Group PR showed a more than 2-fold higher 24-hour survival rate of group LR.
  
  
  CONCLUSIONS
  Pyruvate Ringer solution alleviated organ edema and injury and prompted survival partially through inhibition of lipid peroxidation in various organs in severe HS rats.
authors.affiliation: Department of Burn Surgery, Heilongjiang Provincial Hospital, Harbin 150036, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Laboratory for Shock and Multiple Organ Dysfunction, Key Research Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury and Repair Regeneration, the First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Xian-Qi
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Department of Human Resource, the First Hospital of Harbin, Harbin 150010, China.
authors.forename: Si-Jia
authors.initials: SJ
authors.lastname: Guo
authors.affiliation: Laboratory for Shock and Multiple Organ Dysfunction, Key Research Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury and Repair Regeneration, the First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Laboratory for Shock and Multiple Organ Dysfunction, Key Research Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury and Repair Regeneration, the First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China. Electronic address: bs0425@163.com.
authors.forename: Sen
authors.initials: S
authors.lastname: Hu
authors.affiliation: Shanghai Sandai Pharmarceutical R & D Co, Pudong, Shanghai 201203, China. Electronic address: fqzh60130@yahoo.com.
authors.forename: Fang-Qiang
authors.initials: FQ
authors.lastname: Zhou
authors.affiliation: Laboratory for Shock and Multiple Organ Dysfunction, Key Research Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury and Repair Regeneration, the First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Sheng
mesh_terms: D000818:Animals; D007552:Isotonic Solutions / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology / Q000628:therapy*; D018384:Oxidative Stress / Q000187:drug effects*; D019289:Pyruvic Acid / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation / Q000379:methods*; D000077331:Ringer's Solution; D012771:Shock, Hemorrhagic / Q000150:complications / Q000628:therapy*; D016019:Survival Analysis
chemical_list: D007552:Isotonic Solutions; D000077331:Ringer's Solution; D019289:Pyruvic Acid