title: Comprehensive Chemical Profiling of Picrorhiza kurroa Royle ex Benth Using NMR, HPTLC and LC-MS/MS Techniques.
abstract: Picrorhiza kurroa is an important herb in Indian medicine and contains cucurbitacins, flavonoids, phenolics, iridoid-glucoside and their derivatives as active constituents for the treatment of indigestion, fever, hepatitis, cancer, liver and respiratory diseases. Extensive use of P. kurroa needs detailed analysis and recognition of chemical diversity, is of great importance to evaluate their role as quality control markers. In the present study, comprehensive metabolic profiling of crude extracts of leaves and rhizomes of P. kurroa was carried out using NMR, HPTLC and LC-MS/MS. Primary and secondary metabolites were unambiguously identified along with a new report of monoterpenic glycoside (1-β-D-glucopyranosyl)-8-hydroxy-3,7-dimethyl-oct-2(E),6(E)-dienoate) in P. Kurroa. Significant qualitative differences with respect to the secondary metabolites were noticed between the leaves and rhizomes tissues. Leaves contained more cucurbitacins and flavonoids while iridoids were present more in rhizomes. The comprehensive chemical profiling is expected to give an idea of chemical diversity and quality of P. kurroa, for their ultimate utilisation in various applications.
authors.affiliation: Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061 (HP), India. dineshkumar@ihbt.res.in.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Bikram
authors.initials: B
authors.lastname: Singh
authors.forename: Paramvir Singh
authors.initials: PS
authors.lastname: Ahuja
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D032261:Picrorhiza / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D010936:Plant Extracts