title: Crumbs 2 prevents cortical abnormalities in mouse dorsal telencephalon.
abstract: The formation of a functionally integrated nervous system is dependent on a highly organized sequence of events that includes timely division and differentiation of progenitors. Several apical polarity proteins have been shown to play crucial roles during neurogenesis, however, the role of Crumbs 2 (CRB2) in cortical development has not previously been reported. Here, we show that conditional ablation of Crb2 in the murine dorsal telencephalon leads to defects in the maintenance of the apical complex. Furthermore, within the mutant dorsal telencephalon there is premature expression of differentiation proteins. We examined the physiological function of Crb2 on wild type genetic background as well as on background lacking Crb1. Telencephalon lacking CRB2 resulted in reduced levels of PALS1 and CRB3 from the apical complex, an increased number of mitotic cells and expanded neuronal domain. These defects are transient and therefore only result in rather mild cortical abnormalities. We show that CRB2 is required for maintenance of the apical polarity complex during development of the cortex and regulation of cell division, and that loss of CRB2 results in cortical abnormalities.
authors.affiliation: Department of Neuromedical Genetics, Netherlands Institute for Neuroscience, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands.
authors.forename: Jacobus J
authors.initials: JJ
authors.lastname: Dudok
authors.affiliation: Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom.
authors.forename: Mariyam
authors.initials: M
authors.lastname: Murtaza
authors.affiliation: Department of Neuromedical Genetics, Netherlands Institute for Neuroscience, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Henrique Alves
authors.affiliation: Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom.
authors.forename: Pen
authors.initials: P
authors.lastname: Rashbass
authors.affiliation: Department of Neuromedical Genetics, Netherlands Institute for Neuroscience, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands; Department of Ophthalmology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands. Electronic address: J.Wijnholds@lumc.nl.
authors.forename: Jan
authors.initials: J
authors.lastname: Wijnholds
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018797:Cell Cycle Proteins; D002454:Cell Differentiation; D002455:Cell Division; D002540:Cerebral Cortex / Q000002:abnormalities / Q000196:embryology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018345:Mice, Knockout; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism; D013687:Telencephalon / Q000002:abnormalities* / Q000196:embryology / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; C568885:Crb2 protein, mouse; D008565:Membrane Proteins; C115084:Pard3 protein, mouse; D009703:Nucleoside-Phosphate Kinase; C499979:Mpp5 protein, mouse
keywords: Apical; CRB2; Cortex; Development; PALS1; Polarity