title: Reactive oxygen species regulatory mechanisms associated with rapid response of MC3T3-E1 cells for vibration stress.
abstract: Although many previous studies have shown that refractory period-dependent memory effect of vibration stress is anabolic for skeletal homeostasis, little is known about the rapid response of osteoblasts simply derived from vibration itself. In view of the potential role of reactive oxygen species (ROS) in mediating differentiated activity of osteoblasts, whether and how ROS regulates the rapid effect of vibration deserve to be demonstrated. Our findings indicated that MC3T3-E1 cells underwent decreased gene expression of Runx2, Col-I and ALP and impaired ALP activity accompanied by increased mitochondrial fission immediately after vibration loading. Moreover, we also revealed the involvement of ERK-Drp1 signal transduction in ROS regulatory mechanisms responsible for the rapid effect of vibration stress.
authors.affiliation: Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Xueqi
authors.initials: X
authors.lastname: Gan
authors.affiliation: Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Zhuoli
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China. Electronic address: yhyang6812@scu.edu.cn.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
mesh_terms: D016475:3T3 Cells; D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D020935:MAP Kinase Signaling System / Q000502:physiology; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013314:Stress, Mechanical; D013312:Stress, Physiological / Q000502:physiology*; D014732:Vibration
chemical_list: D017382:Reactive Oxygen Species
keywords: Drp1; Mitochondrial morphology; Osteogenic differentiation; ROS; Vibration