title: Role of ocular albinism type 1 (OA1) GPCR in Asian gypsy moth development and transcriptional expression of heat-shock protein genes.
abstract: The ocular albinism type 1 gene, named OA1, is a coding pigment cell-specific G protein-coupled receptor exclusively localized in intracellular organelles. However, the function of OA1 in insects remains generally unknown. In the present study, we explore for the first time the function of LdOA1 in the Asian gypsy moth, Lymantria dispar. To identify the function of LdOA1 gene in the development and growth of the Asian gypsy moth, the LdOA1 gene in third instar larvae was knocked down by RNAi. Compared with the controls, the knockdown of LdOA1 increased larval mortality but did not significantly affect their utilization of nutrition. Moreover, LdOA1 was stably transformed into the third chromosome of Drosophila melanogaster. The LdOA1 gene in the transformed D. melanogaster modulated the expression of heat-shock protein (hsp) and increased the expression of hsp genes under deltamethrin stress, which indicates that LdOA1 is involved in the regulation of hsp gene expression. These results deepen our understanding of the molecular function of OA1 in insects.
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: LiLi
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: ZhiYing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
authors.forename: HongQu
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: ChuanShan
authors.initials: C
authors.lastname: Zou
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: XuTing
authors.initials: X
authors.lastname: Xue
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China. Electronic address: chuanwangcao@aliyun.com.
authors.forename: ChuanWang
authors.initials: C
authors.lastname: Cao
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D007306:Insecticides / Q000633:toxicity; D008562:Membrane Glycoproteins / Q000235:genetics*; D009036:Moths / Q000235:genetics*; D009570:Nitriles / Q000633:toxicity; D011722:Pyrethrins / Q000633:toxicity; D034622:RNA Interference; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*
chemical_list: D005136:Eye Proteins; D006360:Heat-Shock Proteins; D019476:Insect Proteins; D007306:Insecticides; D008562:Membrane Glycoproteins; D009570:Nitriles; D011722:Pyrethrins; D043562:Receptors, G-Protein-Coupled; C017180:decamethrin
keywords: Asian gypsy moth; Gene function; Heat-shock protein; Ocular albinism type 1