title: Structural characterization of an acidic Epimedium polysaccharide and its immune-enhancement activity.
abstract: One acidic polysaccharide named EPS-1 was isolated from the aqueous extract of the leaves of Epimedium acuminatum Franch. It may be composed of 1,4-linked α-d-GalpA, 1,3,4-linked α-d-GalpA, 1,6-linked β-d-Galp and terminal α-l-Rhap residues in a molar ratio of 11.0:1.0:1.0:1.0 by chemical and spectroscopic analysis. EPS-1 possessed immune modulation effects on peripheral T lymphocyte and immature chBM-DCs such as promoting the proliferation and cytokine secretion of these cells and increasing the phagocytosis ability of immature chBM-DCs.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China. Electronic address: wuyi2001cn@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Youying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Entao
authors.initials: E
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, #483 Wushan Road, Guangzhou 510642, Guangdong Province, PR China.
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Yee
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China. Electronic address: ylhu@njau.edu.cn.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D034181:Epimedium / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D016207:Cytokines; D011134:Polysaccharides
keywords: Epimedium polysaccharide; Immature chBM-DCs; Immune modulation activity; Lymphocyte; Structural characterization; Trifluoroacetic acid (PubChem CID: 6422); d-Fructose (PubChem CID: 5984); d-Galactose (PubChem CID: 6036); d-Galacturonic acid (PubChem CID: 439215); d-Glucose (PubChem CID: 5793); d-Glucuronic acid (PubChem CID: 94715); d-Mannose (PubChem CID: 18950); d-Xylose (PubChem CID: 135191); l-Arabinose (PubChem CID: 439195); l-Rhamnose (PubChem CID: 25310)