title: Expression of antimicrobial peptides under control of a camalexin-biosynthetic promoter confers enhanced resistance against Pseudomonas syringae.
abstract: In Arabidopsis thaliana phytoalexin biosynthesis is tightly regulated. The camalexin biosynthetic gene CYP71B15/PAD3 is highly expressed in response to pathogens and specific abiotic triggers, while constitutive expression is very low. Based on this property we expressed artificial antimicrobial peptides under control of the CYP71B15 promoter avoiding potential toxic effects to the plant related to constitutive expression. Significant and substantial growth inhibition of Pseudomonas syringae was observed, demonstrating that expression of these peptides under control of a phytoalexin promoter is an effective approach for enhancement of resistance against bacterial pathogens.
authors.affiliation: Technische Universität München, LS Genetik, 85354 Freising, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Chapman
authors.affiliation: Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Lindermayr
authors.affiliation: Technische Universität München, LS Genetik, 85354 Freising, Germany. Electronic address: egl@wzw.tum.de.
authors.forename: Erich
authors.initials: E
authors.lastname: Glawischnig
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007211:Indoles; D044224:Pseudomonas syringae / Q000187:drug effects*; D013844:Thiazoles; D000094162:Phytoalexins
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D003577:Cytochrome P-450 Enzyme System; D007211:Indoles; D013844:Thiazoles; C102405:camalexin; D000094162:Phytoalexins; C114108:cytochrome P-450 CYP71B1 (Thlaspi arvense)
keywords: Antimicrobial peptide; Arabidopsis thaliana (Brassicaceae); Inducible promoter; Phytoalexin; Pseudomonas syringae