title: Development and application of an indirect enzyme-linked immunosorbent assay using recombinant truncated Cap protein for the diagnosis of porcine circovirus-like virus P1.
abstract: BACKGROUND
  Porcine circovirus-like virus P1 is a newly discovered virus. To date, there has been no specific serological assay for use in the diagnosis of P1 infection.
  
  
  RESULTS
  Because P1 has high homology to porcine circovirus type 2 (PCV2) at the nucleotide level, the C-terminal portion of the capsid protein (amino acids 73-114), a discriminative antigen, was expressed in a prokaryotic expression system. The recombinant product (rctCap), composed of three identical repeated domains, was shown to be strongly immunoreactive to P1-specific serum. This assay was validated by comparison with an indirect immunofluorescence assay (IFA). The diagnostic sensitivity and specificity of the rctCap enzyme-linked immunosorbent assay (ELISA) developed in this study are 93.6% and 98.3%, respectively, compared with the results from IFAs on 450 sera samples from pigs.
  
  
  CONCLUSIONS
  The indirect ELISA that we developed with rctCap, the recombinant capsid fragment containing the 217-342 nt repeat domain, was sensitive, specific, and suitable for the large-scale detection of P1 infections in swine.
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. wlbwh@hotmail.com.|Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, 210014, China. wlbwh@hotmail.com.|National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China. wlbwh@hotmail.com.
authors.forename: Li-bin
authors.initials: LB
authors.lastname: Wen
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. 503535546@qq.com.|Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, 210014, China. 503535546@qq.com.|National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China. 503535546@qq.com.
authors.forename: Shi-fu
authors.initials: SF
authors.lastname: Wen
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. kwh2003@yeah.net.|Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, 210014, China. kwh2003@yeah.net.|National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China. kwh2003@yeah.net.
authors.forename: Kong-wang
authors.initials: KW
authors.lastname: He
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology
chemical_list: D036022:Capsid Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins