title: Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey.
abstract: We aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium, Onobrychis hypargyrea, and Vicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (α-amylase and α-glucosidase), neurodegenerative disorders (tyrosinase, acetylcholinesterase, and butyrylcholinesterase), and hyperpigmentation (tyrosinase). The antioxidant potential, phenolic and flavonoid content of ethyl acetate, and methanolic and aqueous extracts were investigated using in vitro assays. The total antioxidant capacity (TAC), β-carotene/linoleic acid bleaching activity, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH(•)), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(•+)), cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions were used to evaluate the antioxidant capabilities of the extracts. The half-maximal inhibitory concentrations (IC50) of the extracts on cholinesterase, tyrosinase, and α-amylase were significantly higher than the references, galantamine, kojic acid, and acarbose, respectively. The half-maximal effective concentrations (EC50) of the extracts on TAC, CUPRAC, and FRAP were significantly higher than trolox. The phenol and flavonoid contents of the plant extracts were in the range 20.90 ± 0.190-83.25 ± 0.914 mg gallic acid equivalent/g extract and 1.45 ± 0.200-39.71 ± 0.092 mg rutin equivalent/g extract, respectively. The plants were found to possess moderate antioxidant capacities and interesting inhibitory action against key enzymes.
authors.affiliation: Department of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Zengin
authors.affiliation: Department of Biological Education, Ahmet Keleşoğlu Education Faculty, Necmettin Erbakan University, 42090 Konya, Turkey.
authors.forename: Gokalp Ozmen
authors.initials: GO
authors.lastname: Guler
authors.affiliation: Department of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, Turkey.
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Aktumsek
authors.affiliation: Department of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Ceylan
authors.affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius.
authors.forename: Carene Marie Nancy
authors.initials: CM
authors.lastname: Picot
authors.affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius.
authors.forename: M Fawzi
authors.initials: MF
authors.lastname: Mahomoodally