title: Complete genome sequence of the potato pathogen Ralstonia solanacearum UY031.
abstract: Ralstonia solanacearum is the causative agent of bacterial wilt of potato. Ralstonia solanacearum strain UY031 belongs to the American phylotype IIB, sequevar 1, also classified as race 3 biovar 2. Here we report the completely sequenced genome of this strain, the first complete genome for phylotype IIB, sequevar 1, and the fourth for the R. solanacearum species complex. In addition to standard genome annotation, we have carried out a curated annotation of type III effector genes, an important pathogenicity-related class of genes for this organism. We identified 60 effector genes, and observed that this effector repertoire is distinct when compared to those from other phylotype IIB strains. Eleven of the effectors appear to be nonfunctional due to disruptive mutations. We also report a methylome analysis of this genome, the first for a R. solanacearum strain. This analysis helped us note the presence of a toxin gene within a region of probable phage origin, raising the hypothesis that this gene may play a role in this strain's virulence.
authors.affiliation: Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Guarischi-Sousa
authors.affiliation: Department of Genetics, University of Barcelona and Centre for Research in Agricultural Genomics (CRAG), Bellaterra, Catalonia, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Puigvert
authors.affiliation: Department of Genetics, University of Barcelona and Centre for Research in Agricultural Genomics (CRAG), Bellaterra, Catalonia, Spain.
authors.forename: Núria S
authors.initials: NS
authors.lastname: Coll
authors.affiliation: Departamento de Biociencias, Cátedra de Microbiología, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
authors.forename: María Inés
authors.initials: MI
authors.lastname: Siri
authors.affiliation: Departamento de Biociencias, Cátedra de Microbiología, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
authors.forename: María Julia
authors.initials: MJ
authors.lastname: Pianzzola
authors.affiliation: Department of Genetics, University of Barcelona and Centre for Research in Agricultural Genomics (CRAG), Bellaterra, Catalonia, Spain.
authors.forename: Marc
authors.initials: M
authors.lastname: Valls
authors.affiliation: Instituto de Química, Universidade de São Paulo, São Paulo, Brazil ; Biocomplexity Institute, Virginia Tech, Blacksburg, VA USA.
authors.forename: João C
authors.initials: JC
authors.lastname: Setubal
keywords: Bacterial plant pathogen; Bacterial wilt; Methylome; Ralstonia solanacearum; Short genome report; Uruguay