title: Studying Nestin and its Interrelationship with Cdk5.
abstract: Current research utilizes the specific expression pattern of intermediate filaments (IF) for identifying cellular state and origin, as well as for the purpose of disease diagnosis. Nestin is commonly utilized as a specific marker and driver for CNS progenitor cell types, but in addition, nestin can be found in several mesenchymal progenitor cells, and it is constitutively expressed in a few restricted locations, such as muscle neuromuscular junctions and kidney podocytes. Alike most other members of the IF protein family, nestin filaments are dynamic, constantly being remodeled through posttranslational modifications, which alter the solubility, protein levels, and signaling capacity of the nestin filaments. Through its interactions with kinases and other signaling executors, resulting in a complex and bidirectional regulation of cell signaling events, nestin has the potential to determine whether cells divide, differentiate, migrate, or stay in place. In this review, the broad and similar roles of IFs as dynamic signaling scaffolds, is exemplified by observations of nestin functions and its interaction with the cyclin- dependent kinase 5, the atypical kinase in the family of cyclin-dependent kinases.
authors.affiliation: Cell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland; Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Julia
authors.initials: J
authors.lastname: Lindqvist
authors.affiliation: Cell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland; Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Num
authors.initials: N
authors.lastname: Wistbacka
authors.affiliation: Cell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland; Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland. Electronic address: john.eriksson@abo.fi.
authors.forename: John E
authors.initials: JE
authors.lastname: Eriksson
mesh_terms: D000818:Animals; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism; D064231:Nestin / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D064231:Nestin; D051360:Cyclin-Dependent Kinase 5
keywords: Cdk5; Intermediate filament; Myogenic differentiation; Nestin; Neuromuscular junction; Phosphorylation