title: Identification of Surface Proteins from Lactobacillus casei BL23 Able to Bind Fibronectin and Collagen.
abstract: Strains of lactobacilli show the capacity to attach to extracellular matrix proteins. Cell-wall fractions of Lactobacillus casei BL23 enriched in fibronectin, and collagen-binding proteins were isolated. Mass spectrometry analysis of their protein content revealed the presence of stress-related proteins (GroEL, ClpL), translational elongation factors (EF-Tu, EF-G), oligopeptide solute-binding proteins, and the glycolytic enzymes enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The latter two enzymes were expressed in Escherichia coli and purified as glutathione-S-transferase (GST) fusion proteins, and their in vitro binding activity to fibronectin and collagen was confirmed. These results reinforce the idea that lactobacilli display on their surfaces a variety of moonlighting proteins that can be important in their adaptation to survive at intestinal mucosal sites and in the interaction with host cells.
authors.affiliation: Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Muñoz-Provencio
authors.affiliation: Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
authors.forename: Gaspar
authors.initials: G
authors.lastname: Pérez-Martínez
authors.affiliation: Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain. btcmon@iata.csic.es.
authors.forename: Vicente
authors.initials: V
authors.lastname: Monedero
keywords: Collagen; Fibronectin; Lactobacillus; Surface proteins