title: LC-QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination.
abstract: The purpose of this study was to identify porcine-specific peptide markers from thermally processed meat that could differentiate pork from beef, chevon and chicken meat. In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclaved pork were identified using LC-QTOF-MS. An MRM method was then established for verification. A thorough investigation of LC-QTOF-MS data showed that only seven porcine-specific peptides were consistently detected. Among these peptides, two were derived from lactate dehydrogenase, one from creatine kinase, and four from serum albumin protein. However, MRM could only detect four peptides (EVTEFAK, LVVITAGAR, FVIER and TVLGNFAAFVQK) that were consistently present in pork samples. In conclusion, meat species determination through a tandem mass spectrometry platform shows high potential in providing scientifically valid and reliable results even at peptide level. Besides, the specificity and selectivity offered by the proteomics approach also provide a robust platform for Halal authentication.
authors.affiliation: Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: S A
authors.initials: SA
authors.lastname: Sarah
authors.affiliation: Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: W N
authors.initials: WN
authors.lastname: Faradalila
authors.affiliation: Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: M S
authors.initials: MS
authors.lastname: Salwani
authors.affiliation: Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia; Department of Nutrition and Dietetic, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Amin
authors.affiliation: Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia; University of Malaya Center for Proteomics Research, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: S A
authors.initials: SA
authors.lastname: Karsani
authors.affiliation: Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia; Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia. Electronic address: awis@upm.edu.my.
authors.forename: A Q
authors.initials: AQ
authors.lastname: Sazili
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D000069466:Red Meat / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D010455:Peptides
keywords: MRM and Halal; Mass spectrometry; Peptide marker; Species authentication