title: Isolation and identification of Malassezia species from Chinese and Korean patients with seborrheic dermatitis and in vitro studies on their bioactivity on sebaceous lipids and IL-8 production.
abstract: We investigated the distribution of Malassezia yeast in 120 Chinese (20 patients from each of six cities) and 20 Korean patients with scalp seborrheic dermatitis (SD) and dandruff (SD/D) using ITS1 and ITS2 polymerase chain reaction-restriction fragment length polymorphism. Bioactivity was studied by quantifying sebum lipid production by human primary sebocytes and inflammatory cytokine, interleukin-8 (IL-8) production was studied by exposing HaCaT keratinocytes with extracts of five standard Malassezia strains; M. globosa, M. restricta, M. sympodialis, M. dermatis and M. slooffiae. M. restricta and M. globosa were the most frequently encountered species from both Chinese and Korean patients. These two Malassezia species also promoted neutral lipid synthesis although the result was not statistically significant and induced significant increase in IL-8 production among the five Malassezia species studied. The study suggests a possible role of these organisms in the pathogenesis of SD/D.
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: AMOREPACIFIC Corp. R&D Unit, Yongin, Korea.
authors.forename: Se Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: AMOREPACIFIC Corp. R&D Unit, Yongin, Korea.
authors.forename: Su Na
authors.initials: SN
authors.lastname: Kim
authors.affiliation: AMOREPACIFIC Corp. R&D Unit, Yongin, Korea.
authors.forename: Ah-Reum
authors.initials: AR
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Yu Ri
authors.initials: YR
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: AMOREPACIFIC Corp. R&D Unit, Yongin, Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Park
authors.affiliation: AMOREPACIFIC Corp. R&D Unit, Yongin, Korea.
authors.forename: John Hwan
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Anseong, Korea.
authors.forename: Won Hee
authors.initials: WH
authors.lastname: Jung
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.|Research Institute of Medical Science, Konkuk University, Seoul, Korea.
authors.forename: Yang Won
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.|Research Institute of Medical Science, Konkuk University, Seoul, Korea.
authors.forename: Yong Beom
authors.initials: YB
authors.lastname: Choe
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.|Research Institute of Medical Science, Konkuk University, Seoul, Korea.
authors.forename: Kyu Joong
authors.initials: KJ
authors.lastname: Ahn
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002681:China; D004271:DNA, Fungal / Q000302:isolation & purification; D021901:DNA, Intergenic / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D063807:Dandruff / Q000382:microbiology; D012628:Dermatitis, Seborrheic / Q000382:microbiology*; D005260:Female; D016681:Genome, Fungal / Q000235:genetics; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D015603:Keratinocytes / Q000166:cytology; D008055:Lipids / Q000096:biosynthesis; D008290:Malassezia / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D012536:Scalp Dermatoses / Q000382:microbiology*; D012629:Sebum / Q000378:metabolism*; D066106:Seoul; D014505:Urban Population
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D016209:Interleukin-8; D008055:Lipids; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
keywords: IL-8; ITS1 and ITS2 PCR-RFLP; Malassezia yeast; dandruff; neutral lipid synthesis; seborrheic dermatitis