title: A Novel Wistar Rat Model of Obesity-Related Nonalcoholic Fatty Liver Disease Induced by Sucrose-Rich Diet.
abstract: The pathogenesis of nonalcoholic fatty liver disease (NAFLD) is not fully understood, and experimental models are an alternative to study this issue. We investigated the effects of a simple carbohydrate-rich diet on the development of obesity-related NAFLD and the impact of physical training on the metabolic abnormalities associated with this disorder. Sixty Wistar rats were randomly separated into experimental and control groups, which were fed with sucrose-enriched (18% simple carbohydrates) and standard diet, respectively. At the end of each experimental period (5, 10, 20, and 30 weeks), 6 animals from each group were sacrificed for blood tests and liver histology and immunohistochemistry. From weeks 25 to 30, 6 animals from each group underwent physical training. The experimental group animals developed obesity and NAFLD, characterized histopathologically by steatosis and hepatocellular ballooning, clinically by increased thoracic circumference and body mass index associated with hyperleptinemia, and metabolically by hyperglycemia, hyperinsulinemia, hypertriglyceridemia, increased levels of very low-density lipoprotein- (VLDL-) cholesterol, depletion of the antioxidants liver enzymes superoxide dismutase and catalase, and increased hepatic levels of malondialdehyde, an oxidative stress marker. Rats that underwent physical training showed increased high-density lipoprotein- (HDL-) cholesterol levels. In conclusion, a sucrose-rich diet induced obesity, insulin resistance, oxidative stress, and NAFLD in rats.
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Maria Luíza R P
authors.initials: ML
authors.lastname: Lima
authors.affiliation: Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Laura H R
authors.initials: LH
authors.lastname: Leite
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Carreiros, 96203-900 Rio Grande, RS, Brazil.
authors.forename: Carolina R
authors.initials: CR
authors.lastname: Gioda
authors.affiliation: Departamento de Veterinária Clínica e Cirúrgica, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Fabíola O P
authors.initials: FO
authors.lastname: Leme
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Couto
authors.affiliation: Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Cândido C
authors.initials: CC
authors.lastname: Coimbra
authors.affiliation: Departamento de Anatomia Patológica e Medicina Legal, Faculdade de Medicina, Universidade Federal de Minas Gerais, 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Virginia H R
authors.initials: VH
authors.lastname: Leite
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Teresa Cristina A
authors.initials: TC
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D002374:Catalase / Q000378:metabolism; D019422:Dietary Sucrose; D004195:Disease Models, Animal; D005081:Exercise Therapy / Q000379:methods; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000209:etiology* / Q000473:pathology / Q000628:therapy; D009765:Obesity / Q000097:blood / Q000209:etiology* / Q000473:pathology / Q000628:therapy; D018384:Oxidative Stress; D017208:Rats, Wistar; D012420:Running; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D019422:Dietary Sucrose; D007328:Insulin; D020738:Leptin; D008055:Lipids; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase