title: Influence of magnesium sulfate on HCO3/Cl transmembrane exchange rate in human erythrocytes.
abstract: Magnesium sulfate (MgSO4) is widely used in medicine but molecular mechanisms of its protection through influence on erythrocytes are not fully understood and are considerably controversial. Using scanning flow cytometry, in this work for the first time we observed experimentally (both in situ and in vitro) a significant increase of HCO3(-)/Cl(-) transmembrane exchange rate of human erythrocytes in the presence of MgSO4 in blood. For a quantitative analysis of the obtained experimental data, we introduced and verified a molecular kinetic model, which describes activation of major anion exchanger Band 3 (or AE1) by its complexation with free intracellular Mg(2+) (taking into account Mg(2+) membrane transport and intracellular buffering). Fitting the model to our in vitro experimental data, we observed a good correspondence between theoretical and experimental kinetic curves that allowed us to evaluate the model parameters and to estimate for the first time the association constant of Mg(2+) with Band 3 as KB~0.07mM, which is in agreement with known values of the apparent Mg(2+) dissociation constant (from 0.01 to 0.1mM) that reflects experiments on enrichment of Mg(2+) at the inner erythrocyte membrane (Gunther, 2007). Results of this work partly clarify the molecular mechanisms of MgSO4 action in human erythrocytes. The method developed allows one to estimate quantitatively a perspective of MgSO4 treatment for a patient. It should be particularly helpful in prenatal medicine for early detection of pathologies associated with the risk of fetal hypoxia.
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia.
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Chernyshova
authors.affiliation: Medical Centre of Siberian Branch of the Russian Academy of Science, Pirogova 25, 630090 Novosibirsk, Russia.
authors.forename: Yulia S
authors.initials: YS
authors.lastname: Zaikina
authors.affiliation: ANO "Center of New Medical Technologies in Akademgorodok", Pirogova 25/4, 630090 Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Avenue 8, 630090 Novosibirsk, Russia.
authors.forename: Galina A
authors.initials: GA
authors.lastname: Tsvetovskaya
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, 630090 Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091 Novosibirsk, Russia.
authors.forename: Dmitry I
authors.initials: DI
authors.lastname: Strokotov
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia.
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Yurkin
authors.affiliation: Medical Centre of Siberian Branch of the Russian Academy of Science, Pirogova 25, 630090 Novosibirsk, Russia.
authors.forename: Elena S
authors.initials: ES
authors.lastname: Serebrennikova
authors.affiliation: Centre de Recherches Cliniques Etienne-Le Bel, 3001 12 Av. Nord Sherbrooke loc. # 4867, Quebec, Canada J1H 5N4.
authors.forename: Leonid
authors.initials: L
authors.lastname: Volkov
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091 Novosibirsk, Russia.
authors.forename: Valeri P
authors.initials: VP
authors.lastname: Maltsev
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia. Electronic address: chern@ns.kinetics.nsc.ru.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Chernyshev
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D027963:Chloride-Bicarbonate Antiporters / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008954:Models, Biological; D014019:Tissue Donors
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D027963:Chloride-Bicarbonate Antiporters; D008278:Magnesium Sulfate
keywords: Band 3; Kinetic modeling; Magnesium sulfate; Red blood cells