title: Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging.
abstract: For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.
authors.forename: H D
authors.initials: HD
authors.lastname: Coughlan
authors.affiliation: Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University , Melbourne 3086, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Darmanin
authors.forename: N W
authors.initials: NW
authors.lastname: Phillips
authors.affiliation: Department of Engineering Science, University of Oxford , Oxford OX1 3PJ, United Kingdom.
authors.forename: F
authors.initials: F
authors.lastname: Hofmann
authors.forename: J N
authors.initials: JN
authors.lastname: Clark
authors.affiliation: Advanced Photon Source, Argonne National Laboratory , Argonne, Illinois 60439, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Harder
authors.affiliation: Advanced Photon Source, Argonne National Laboratory , Argonne, Illinois 60439, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Vine
authors.forename: B
authors.initials: B
authors.lastname: Abbey