title: Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
abstract: A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species. Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap. Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation. The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: Bao Quoc
authors.initials: BQ
authors.lastname: Tran
authors.affiliation: MedImmune, LLC, Gaithersburg, MD, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Barton
authors.affiliation: MedImmune, LLC, Gaithersburg, MD, USA.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Feng
authors.affiliation: MedImmune, LLC, Gaithersburg, MD, USA.
authors.forename: Aimee
authors.initials: A
authors.lastname: Sandjong
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Yoon
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: Shivangi
authors.initials: S
authors.lastname: Awasthi
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Liang
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: Mohd M
authors.initials: MM
authors.lastname: Khan
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: David P A
authors.initials: DP
authors.lastname: Kilgour
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Goo