title: [Resveratrol increases sirtuin 1 expression in peripheral blood mononuclear cells of premature infants and inhibits the oxidative stress induced by hyperoxia in vivo].
abstract: OBJECTIVE
  To explore the effect of resveratrol on the levels of sirtuin 1 (SIRT1) and reactive oxygen species (ROS) in peripheral blood mononuclear cells (PBMCs) of premature infants exposed to hyperoxia.
  
  
  METHODS
  Peripheral blood and isolated PBMCs from premature infants (gestational age<32 weeks) without oxygen supplement were collected and were randomly assigned into four groups: control, air+resveratrol, hyperoxia, and hyperoxia+resveratrol. The PBMCs were cultured in vitro for 48 hours, then the ROS content in PBMCs was measured by laser scanning confocal microscopy. Malondialdehyde (MDA) content in the medium was measured by the whole spectrum spectrophotometer. SIRT1 positioning was assessed by immunofluorescence. SIRT1 expression levels in PBMCs were measured by Western bolt.
  
  
  RESULTS
  Compared with the control group, the level of SIRT1 in the air+resveratrol group increased significantly (P<0.05). The levels of ROS and MDA and the SIRT1 transposition rate in the hyperoxia group increased significantly, while the expression level of SIRT1 decreased significantly compared with the control group (P<0.05). The levels of ROS and MDA and the SIRT1 transposition rate decreased significantly (P<0.05), and the expression level of SIRT1 increased significantly in the hyperoxia+resveratrol group (P<0.05).
  
  
  CONCLUSIONS
  Resveratrol can increase SIRT1 expression in PBMCs and inhibit SIRT1 shuttle from nucleus to cytoplasm in order to increase the ability of antioxidative stress in premature infants exposed to hyperoxia, thereby reducing the oxidative stress injury in premature infants.
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, China. dongwenbin2000@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Dong
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Kang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Lei
authors.forename: Lian-Yu
authors.initials: LY
authors.lastname: Zhang
authors.forename: You-Ying
authors.initials: YY
authors.lastname: Lu
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Zhai
mesh_terms: D005260:Female; D006801:Humans; D018496:Hyperoxia / Q000378:metabolism*; D007231:Infant, Newborn; D007234:Infant, Premature; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000097:blood*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; D056564:Sirtuin 1; D000077185:Resveratrol