title: Monoliths in capillary electrochromatography and capillary liquid chromatography in conjunction with mass spectrometry.
abstract: Here, we have reviewed separation studies utilizing monolithic capillary columns for separation of compounds preceding MS analysis. The review is divided in two parts according to the used separation method, namely CEC and capillary LC (cLC). Based on our overview, monolithic CEC-MS technique have been more focused on the syntheses of highly specialized and selective separation phase materials for fast and efficient separation of specific types of analytes. In contrast, monolithic cLC-MS is more widely used and is often employed, for instance, in the analysis of oligonucleotides, metabolites, and peptides and proteins in proteomic studies. While poly(styrene-divinylbenzene)-based and silica-based monolithic capillaries found their place in proteomic analyses, the other laboratory-synthesized monoliths still wait for their wider utilization in routine analyses. The development of new monolithic materials will most likely continue due to the demand of more efficient and rapid separation of increasingly complex samples.
authors.affiliation: Institute of Analytical Chemistry of the CAS, v. v. i, Brno, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Moravcová
authors.affiliation: Department of Chemistry, University of Helsinki, Helsinki, Finland.
authors.forename: Antti H
authors.initials: AH
authors.lastname: Rantamäki
authors.affiliation: Institute of Analytical Chemistry of the CAS, v. v. i, Brno, Czech Republic.
authors.forename: Filip
authors.initials: F
authors.lastname: Duša
authors.affiliation: Department of Chemistry, University of Helsinki, Helsinki, Finland.
authors.forename: Susanne K
authors.initials: SK
authors.lastname: Wiedmer
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis; D053801:Capillary Electrochromatography; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D009841:Oligonucleotides / Q000032:analysis; D011506:Proteins / Q000032:analysis
chemical_list: D001647:Bile Acids and Salts; D009841:Oligonucleotides; D011506:Proteins
keywords: Capillary electrochromatography; Capillary liquid chromatography; Mass spectrometry; Monolithic columns