title: Expression of ZmGA20ox cDNA alters plant morphology and increases biomass production of switchgrass (Panicum virgatum L.).
abstract: Switchgrass (Panicum virgatum L.) is considered a model herbaceous energy crop for the USA, for its adaptation to marginal land, low rainfall and nutrient-deficient soils; however, its low biomass yield is one of several constraints, and this might be rectified by modulating plant growth regulator levels. In this study, we have determined whether the expression of the Zea mays gibberellin 20-oxidase (ZmGA20ox) cDNA in switchgrass will improve biomass production. The ZmGA20ox gene was placed under the control of constitutive CaMV35S promoter with a strong TMV omega enhancer, and introduced into switchgrass via Agrobacterium-mediated transformation. The transgene integration and expression levels of ZmGA20ox in T0 plants were analysed using Southern blot and qRT-PCR. Under glasshouse conditions, selected transgenic plants exhibited longer leaves, internodes and tillers, which resulted in twofold increased biomass. These phenotypic alterations correlated with the levels of transgene expression and the particular gibberellin content. Expression of ZmGA20ox also affected the expression of genes coding for key enzymes in lignin biosynthesis. Our results suggest that the employment of ectopic ZmGA20ox and selection for natural variants with high level expression of endogenous GA20ox are appropriate approaches to increase biomass production of switchgrass and other monocot biofuel crops.
authors.affiliation: Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Phat T
authors.initials: PT
authors.lastname: Do
authors.affiliation: Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Joann R
authors.initials: JR
authors.lastname: De Tar
authors.affiliation: Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Michelle K
authors.initials: MK
authors.lastname: Folta
authors.affiliation: Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Zhanyuan J
authors.initials: ZJ
authors.lastname: Zhang
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D048429:Cell Size; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D008897:Panicum / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000254:growth & development; D003313:Zea mays / Q000235:genetics
chemical_list: D056804:Biofuels; D018076:DNA, Complementary
keywords: biofuel; biomass; gibberellin; gibberellin 20-oxidase; switchgrass