title: Rorippa indica Regeneration via Somatic Embryogenesis Involving Frog Egg-like Bodies Efficiently Induced by the Synergy of Salt and Drought Stresses.
abstract: Frog egg-like bodies (FELBs), novel somatic embryogenesis (SE) structures first observed in Solanum nigrum, were induced in Rorippa indica. NaCl-mediated salt and mannitol-mimicked drought stresses induced FELBs in R. indica, which is very different from the induction by plant growth regulators (PGRs) under low light condition that was used in S. nigrum FELB induction. It demonstrated that NaCl or mannitol supplements alone could induce FELBs in R. indica, but with low induction rates, while the synergy of NaCl and mannitol significantly increased the FELB induction rates. For the combination of 5.0 g/L mannitol and 10.0 g/L NaCl the highest FELB induction rate (100%) was achieved. It suggests that the synergy of drought and salt stresses can replace PGRs to induce FELBs in R. indica. On medium supplemented with 1.0 mg/L gibberellic acid all the inoculated in vitro FELBs developed into multiple plantlets. Morphological and histological analyses confirmed the identity of FELBs induced in R. indica and revealed that FELBs originate from root cortex cells.
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Kedong
authors.initials: K
authors.lastname: Xu
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Zhanshuai
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Shuya
authors.initials: S
authors.lastname: Ma
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Yuexing
authors.initials: Y
authors.lastname: Xin
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Chunjing
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Qianbei
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Hanhan
authors.initials: H
authors.lastname: Qiu
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Pi
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Youwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Guangxuan
authors.initials: G
authors.lastname: Tan
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Li
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000818:Animals; D001001:Anura; D055864:Droughts; D005629:Frozen Sections; D007210:Indoleacetic Acids / Q000494:pharmacology; D008027:Light; D008353:Mannitol / Q000494:pharmacology; D010063:Ovum; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D012038:Regeneration / Q000187:drug effects* / Q000528:radiation effects; D031225:Rorippa / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000528:radiation effects
chemical_list: D007210:Indoleacetic Acids; D015084:2,4-Dichlorophenoxyacetic Acid; D008353:Mannitol; D012965:Sodium Chloride; C030737:indoleacetic acid