title: Characterization and Antilisterial Effect of Phosphatidylcholine Nanovesicles Containing the Antimicrobial Peptide Pediocin.
abstract: Encapsulation may provide increased stability and antimicrobial efficiency to bacteriocins. In this work, the antilisterial peptide pediocin was encapsulated in nanovesicles prepared from partially purified soybean phosphatidylcholine. The maintenance of antimicrobial activity and properties of free and encapsulated pediocin was observed during 13 days at 4 °C, and after this period, the encapsulated pediocin retained 50 % its initial activity. The maintenance of the bioactive properties of free and encapsulated pediocin was observed against different species of Listeria, inhibiting Listeria monocytogenes, Listeria innocua and Listeria ivanovii. The size of vesicles containing pediocin was determined by dynamic light scattering as an average of 190 nm, with little change throughout the observation period. Polydispersity index values were around 0.201 and are considered satisfactory, indicating an adequate size distribution of liposomes. The efficiency of encapsulation was 80 %. Considering these results, the protocol used was appropriate for the encapsulation of this bacteriocin. Results demonstrate the production of stable nanoparticulate material. The maintenance of the properties of pediocin encapsulated in liposomes is fundamental to prospect the stability in different conditions of the food matrix.
authors.affiliation: Laboratório de Inspeção de Produtos de Origem Animal, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: Michele Brauner
authors.initials: MB
authors.lastname: de Mello
authors.affiliation: Laboratório de Microbiologia e Bioquímica Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: da Silva Malheiros
authors.affiliation: Laboratório de Microbiologia e Bioquímica Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli
authors.affiliation: Laboratório de Instrumentação e Dinâmica Molecular, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Nádya
authors.initials: N
authors.lastname: Pesce da Silveira
authors.affiliation: Inspeção e Tecnologia de Leite e Derivados, Ovos e Mel. Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Márcia Monks
authors.initials: MM
authors.lastname: Jantzen
authors.affiliation: Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, sala 158, 90050-170, Porto Alegre, Brazil. amanda.motta@ufrgs.br.
authors.forename: Amanda
authors.initials: A
authors.lastname: de Souza da Motta
keywords: Bacteriocin; Encapsulation; Liposome; Nanovesicles; Pediocin