title: Dynamic Changes in Occupancy of Histone Variant H2A.Z during Induced Somatic Cell Reprogramming.
abstract: The development of induced pluripotent stem cells (iPSCs) has enabled study of the mechanisms underlying cellular reprogramming. Here, we have studied the dynamic distribution of H2A.Z during induced reprogramming with chromatin immunoprecipitation deep sequencing (ChIP-Seq). We found that H2A.Z tended to accumulate around transcription start site (TSS) and incorporate in genes with a high transcriptional activity. GO analysis with H2A.Z incorporated genes indicated that most genes are involved in chromatin assembly or disassembly and chromatin modification both in MEF and Day 7 samples, not in iPSCs. Furthermore, we detected the highest level of incorporation of H2A.Z around TSS in Day 7 samples compared to MEFs and iPSCs. GO analysis with only incorporated genes in Day 7 also displayed the function of chromatin remodeling. So, we speculate H2A.Z may be responsible for chromatin remodeling to enhance the access of transcription factors to genes important for pluripotency. This study therefore provides a deeper understanding of the mechanisms underlying induced reprogramming.
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China; Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Fulu
authors.initials: F
authors.lastname: Dong
authors.affiliation: Medical College, Hunan Normal University, Changsha 410006, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Song
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Baole
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: BaoChun
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Jinling Hospital, Medical School of Nanjing University, Nanjing 210093, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Youde
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Song
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Peiqing
authors.initials: P
authors.lastname: Cong
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu