title: Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
abstract: This study is to investigate the effects of human umbilical cord-mesenchymal stem cells (HUC-MSCs) transplantation combined with minimally invasive hematoma aspiration on neural functional recovery and p53 gene expression in rats with intracerebral hemorrhage (ICH). Collagenase type-IV was injected to the caudate nucleus of the rats to make ICH models. One hundred and twenty Sprague-Dawley rats with successful modeling were randomly divided into 4 groups, including the ICH group, hematoma aspiration group, HUC-MSCs transplantation group and HUC-MSCs transplantation combined with hematoma aspiration group (combination group). Neural functional status of the rats was assessed by modified neurological severity score (mNSS). Expression of p53 in the cerebral tissues surrounding ICH was detected by immunohistochemical assays. The scores of mNSS and the expression of p53 gene in the hematoma aspiration group, the HUC-MSCs transplantation group and the combination group were significantly lower than those in the ICH group at each indicated time point (p < 0.05). Intriguingly, mNSS scores and p53 expression in the combination group were significantly lower than those in the hematoma aspiration group on day 7, 14 and 30 (p < 0.05), and significantly lower than those in the HUC-MSCs transplantation group on day 14 and 30 (p < 0.05). HUC-MSCs transplantation combined with minimally invasive hematoma aspiration is more effective than either therapy alone in rats with ICH and could distinctly reduce the damage of nerve cells.
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Shang
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Hao
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Maoyong
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College Yantai 264000, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Yuanxiao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P.R. China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
keywords: Human umbilical cord-mesenchymal stem cell transplantation; intracerebral hemorrhage; minimally invasive hematoma aspiration; p53; rats