title: E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion.
abstract: BACKGROUND
  Adiponectin (APN) is an adipocyte-derived bioactive molecule with anti-diabetic and anti-atherogenic properties. Although anti-diabetic effects are mostly mediated by the adiponectin receptors AdipoR1 and AdipoR2, the anti-atherogenic mechanisms have not been fully elucidated.
  
  
  RESULTS
  In this study, we identified E-selectin ligand (ESL)-1 as a novel APN-binding protein by mass spectrometry analysis of HepG2 cell-derived immunoprecipitant with an anti-APN antibody. Cell adhesion assays using fluorescence-labelled monocyte cell line THP-1 cells and human umbilical vein endothelial cells (HUVECs) revealed that APN-pre-treated THP-1 cells had reduced binding ability to HUVECs. This APN-mediated suppressive effect on monocyte binding to endothelial cells was partially abrogated by targeting ESL-1 with shRNA in THP-1 cells. In addition, serial mutagenesis analysis disclosed that five extracellular amino acids close to the N-terminus of ESL-1 were essential for binding with APN.
  
  
  CONCLUSIONS
  Our results highlight the fact that interaction between APN and ESL-1 could provide a fundamental mechanism underlying the anti-atherogenic properties of APN.
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Kuroda
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Uekita
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Ikoi
authors.initials: I
authors.lastname: Kochi
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Niinaga
authors.affiliation: Department of Metabolic Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.affiliation: Department of Metabolic Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Iichiro
authors.initials: I
authors.lastname: Shimomura
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan. Electronic address: skihara@sahs.med.osaka-u.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kihara
mesh_terms: D052242:Adiponectin / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D056945:Hep G2 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D011485:Protein Binding; D017468:Receptors, Fibroblast Growth Factor / Q000737:chemistry / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D015815:Cell Adhesion Molecules; D017468:Receptors, Fibroblast Growth Factor; D012795:Sialoglycoproteins; C086711:cysteine-rich fibroblast growth factor receptor
keywords: Adhesion; Adiponectin; Atherosclerosis; Cell–cell interaction; ESL-1