title: A novel thermostable and organic solvent-tolerant lipase from Xanthomonas oryzae pv. oryzae YB103: screening, purification and characterization.
abstract: Thermostable lipases offer major biotechnological advantages over mesophilic lipases. In this study, an intracellular thermostable and organic solvent-tolerant lipase-producing strain YB103 was isolated from soil samples and identified taxonomically as Xanthomonas oryzae pv. oryzae. The lipase from X. oryzae pv. oryzae YB103 (LipXO) was purified 101.1-fold to homogeneity with a specific activity of 373.9 U/mg. The purified lipase showed excellent thermostability, exhibiting 51.1% of its residual activity after incubation for 3 days at 70 °C. The enzyme showed optimal activity at 70 °C, suggesting it is a thermostable lipase. LipXO retained 75.1-154.1% of its original activity after incubation in 20% (v/v) hydrophobic organic solvents at 70 °C for 24 h. Furthermore, LipXO displayed excellent stereoselectivity (e.e.p >99%) toward (S)-1-phenethyl alcohol in n-hexane. These unique properties of LipXO make it promising as a biocatalyst for industrial processes.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Qiurun
authors.initials: Q
authors.lastname: Mo
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Aili
authors.initials: A
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Hailun
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China. limu@mail.hzau.edu.cn.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China. limu@mail.hzau.edu.cn.|College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China. limu@mail.hzau.edu.cn.
authors.forename: Mu
authors.initials: M
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D004795:Enzyme Stability; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D009930:Organic Chemicals; D012997:Solvents; D008049:Lipase; C428386:thermostable lipase
keywords: Biotechnology; Enzyme application; Enzyme stability; Enzyme structure; Function; Protein stability; Synthesis in organic solvents