title: Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth.
abstract: Rbm47 encodes a RNA binding protein that is necessary for Cytidine to Uridine RNA editing. Rbm47(gt/gt) mutant mice that harbor inactivated Rbm47 display poor viability. Here it was determined that the loss of Rbm47(gt/gt) offspring is due to embryonic lethality at mid-gestation. It was further showed that growth of the surviving Rbm47(gt/gt) mutants is impaired. Rbm47 is expressed in both the visceral endoderm and the definitive endoderm. Using the utility of the switchable FlEx gene-trap cassette and the activity of Cre and FLP recombinases to generate mice that conditionally inactivate and restore Rbm47 function in tissue-specific manner, it was demonstrated that Rbm47 function is required in the embryo proper, and not the visceral endoderm, for viability and growth. genesis 54:115-122, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, New South Wales, 2145, Australia.|Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales, 2006, Australia.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fossat
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, New South Wales, 2145, Australia.
authors.forename: Tania
authors.initials: T
authors.lastname: Radziewic
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, New South Wales, 2145, Australia.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Jones
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, New South Wales, 2145, Australia.
authors.forename: Karin
authors.initials: K
authors.lastname: Tourle
authors.affiliation: Embryology Unit, Children's Medical Research Institute, Westmead, New South Wales, 2145, Australia.|Discipline of Medicine, Sydney Medical School, University of Sydney, New South Wales, 2006, Australia.
authors.forename: Patrick P L
authors.initials: PP
authors.lastname: Tam
mesh_terms: D000818:Animals; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal; D005818:Genetic Engineering; D051379:Mice; D009154:Mutation; D009928:Organ Specificity; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D016019:Survival Analysis
chemical_list: D016601:RNA-Binding Proteins; C585951:Rbm47 protein, mouse
keywords: Cre; FLP; FlEx system; Rbm47; definitive endoderm; mouse; visceral endoderm