title: Role of phosphate solubilizing Burkholderia spp. for successful colonization and growth promotion of Lycopodium cernuum L. (Lycopodiaceae) in lateritic belt of Birbhum district of West Bengal, India.
abstract: Profuse growth of Lycpodium cernuum L. was found in phosphate deficient red lateritic soil of West Bengal, India. Interaction of vesicular-arbuscular mycorrhiza (VAM) with Lycopodium rhizoids were described earlier but association of PGPR with their rhizoids were not studied. Three potent phosphate solubilizing bacterial strains (P4, P9 and P10) associated with L. cernuum rhizoids were isolated and identified by 16S rDNA homologies on Ez-Taxon database as Burkholderia tropica, Burkholderia unamae and Burkholderia cepacia respectively. Day wise kinetics of phosphate solubilization against Ca3(PO4)2 suggested P4 (580.56±13.38 μg ml(-1)) as maximum mineral phosphate solubilizer followed by P9 (517.12±17.15 μg ml(-1)) and P10 (485.18±14.23 μg ml(-1)) at 28 °C. Release of bound phosphates by isolated strains from ferric phosphate (FePO4), aluminum phosphate (AlPO4) and four different complex rock phosphates indicated their very good phosphate solubilizng efficacy. Nitrogen independent solubilizition also supports their nitrogen fixing capabilities. Inhibition of P solubilization by calcium salts and induction by EDTA suggested pH dependent chelation of metal cations by all of the isolates. Rhizoidal colonization potentials of Burkholderia spp. were confirmed by in planta experiment and also using scanning electron microscope (SEM). Increases of total phosphate content in Lycopodium plants upon soil treatment with these isolates were also recorded. In addition siderophore production on CAS agar medium, tryptophan dependent IAA production and antifungal activities against pathogenic fungi by rhizospheric isolates deep-rooted that they have definite role in nutrient mobilization for successful colonization of L. cernuum in nutrient deficient lateritic soil.
authors.affiliation: Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati, Santiniketan-731235, West Bengal, India. Electronic address: vbranjan@gmail.com.
authors.forename: Ranjan
authors.initials: R
authors.lastname: Ghosh
authors.affiliation: Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati, Santiniketan-731235, West Bengal, India. Electronic address: vb.somabarman@gmail.com.
authors.forename: Soma
authors.initials: S
authors.lastname: Barman
authors.affiliation: Department of Biotechnology, Daegu University, South Korea. Electronic address: rajibmukherjee.vb@gmail.com.
authors.forename: Rajib
authors.initials: R
authors.lastname: Mukherjee
authors.affiliation: Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati, Santiniketan-731235, West Bengal, India. Electronic address: mandalnc@rediffmail.com.
authors.forename: Narayan C
authors.initials: NC
authors.lastname: Mandal
mesh_terms: D017607:Aluminum Compounds / Q000378:metabolism; D019117:Burkholderia / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D032502:Lycopodium / Q000737:chemistry / Q000254:growth & development* / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D038821:Mycorrhizae; D009414:Nerve Growth Factors; D009586:Nitrogen Fixation; D010710:Phosphates / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017262:Siderophores / Q000096:biosynthesis; D012988:Soil Microbiology
chemical_list: D017607:Aluminum Compounds; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007210:Indoleacetic Acids; D009414:Nerve Growth Factors; D010710:Phosphates; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; C012714:aluminum phosphate
keywords: Kinetics of solubilization; Lycopodium cernuum L.; Phosphate solubilizing bacteria; Plant growth promoting activity; Rhizoidal colonization; Rock phosphates