title: Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection.
abstract: Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in contrast to its revertant, could not be reconstituted. Moreover, deletion of gB cytoplasmic tail impaired the intracellular transport of gB protein to the trans-Golgi network (TGN). Taken together, these results suggest that gB CTD is critical for HHV-6 propagation and important for intracellular transportation.
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors.forename: Nora F
authors.initials: NF
authors.lastname: Mahmoud
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Indonesia.
authors.forename: Chyntia
authors.initials: C
authors.lastname: Jasirwan
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kanemoto
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Aika
authors.initials: A
authors.lastname: Wakata
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Bochao
authors.initials: B
authors.lastname: Wang
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Hata
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagamata
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawabata
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Department of Immunology, Nanjing Medical University, Nanjing, China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Tang
authors.affiliation: Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan. Electronic address: ymori@med.kobe-u.ac.jp.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
mesh_terms: D015654:Herpesvirus 6, Human / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D017434:Protein Structure, Tertiary; D019349:Roseolovirus Infections / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D021601:trans-Golgi Network / Q000821:virology
chemical_list: D014759:Viral Envelope Proteins; C077311:glycoprotein B, human herpesvirus-6
keywords: Cytoplasmic tail domain; Glycoprotein B; HHV-6; Intracellular transport