title: Characterisation of hydrolysates prepared from engraved catfish (Nemapteryx caelata) roe by serial hydrolysis.
abstract: Protein hydrolysates were prepared from defatted engraved catfish roe using alcalase enzyme by a two-stage serial hydrolysis process. The soluble hydrolysate formed after first stage of hydrolysis was removed (RH-1) and fresh enzyme was added at the same concentration to achieve further hydrolysis (RH-2). Further, compositional, surface-active and antioxidant properties of both hydrolysates were compared. The SDS-PAGE profile showed two distinct bands for RH-1, whereas no bands were visible for RH-2. On the other hand, gel filtration chromatography of the hydrolysates indicated 3-4 distinct fractions. Both the hydrolysates showed similar foam forming abilities, however, RH-1 exhibited poor foam stability. Emulsion properties of RH-1 were superior to that of RH-2. The major fractions eluted through gel filtration column were screened for antioxidant properties. Higher DPPH radical scavenging and metal chelating properties were observed for RH-1 second fragment, whereas FRAP and Fe(2+) reducing power was highest for second fragment of RH-2.
authors.affiliation: Mumbai Research Centre of Central Institute of Fisheries Technology, Vashi, Navi Mumbai 400 703 India.
authors.forename: P K
authors.initials: PK
authors.lastname: Binsi
authors.affiliation: Mumbai Research Centre of Central Institute of Fisheries Technology, Vashi, Navi Mumbai 400 703 India.
authors.forename: P
authors.initials: P
authors.lastname: Viji
authors.affiliation: Central Institute of Fisheries Technology, Matsyapuri, Willington Island, Cochin 682 029 India.
authors.forename: Satyen Kumar
authors.initials: SK
authors.lastname: Panda
authors.affiliation: Central Institute of Fisheries Technology, Matsyapuri, Willington Island, Cochin 682 029 India.
authors.forename: Suseela
authors.initials: S
authors.lastname: Mathew
authors.affiliation: Central Institute of Fisheries Technology, Matsyapuri, Willington Island, Cochin 682 029 India.
authors.forename: A A
authors.initials: AA
authors.lastname: Zynudheen
authors.affiliation: Central Institute of Fisheries Technology, Matsyapuri, Willington Island, Cochin 682 029 India.
authors.forename: C N
authors.initials: CN
authors.lastname: Ravishankar
keywords: Antioxidant activity; Catfish roe; Functional properties; Hydrolysates; Serial hydrolysis