title: [Isoflavonoids from Caragana changduensis and their nitric oxideinhibitory activities].
abstract: Ten isoflavonoids were isolated from the heartwoods of Caragana changduensis Lion f. by means of various column chromatographic techniques. Based on the detailed spectral data analysis (MS and NMR), as well as comparison with the literatures, their chemical structures were determined as 7,2'-dihydroxy-8,4'-dimethoxyisoflavone (1), 4'-hydroxy-7,3'-dimethoxyisoflavone (2), 5, 7, 4'-trihydroxy-2',5'-dimethoxyisoflavone (3), prunetin (4), afrormosin (5), odoratin (6), genistein (7), texasin (8), pratensein (9), and 6,7,3'-trihydroxy-4'-methoxyisoflavone (10). Among them, compounds 1-3 and 9-10 were isolated from the Caragana genus for the first time. All the compounds were obtained from this species for the first time. In the preliminary assays, compounds 1, 2, 6, and 7 possessed significant inhibitory effects on NO production, with IC50 values of 48.12, 25.32, 62.71, 43.59 μmol x L(-1), respectively.
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Sun
authors.forename: Shi-ming
authors.initials: SM
authors.lastname: Fang
authors.forename: Mao-dan
authors.initials: MD
authors.lastname: Zang
authors.forename: Cheng-xiong
authors.initials: CX
authors.lastname: Yang
authors.forename: He-ran
authors.initials: HR
authors.lastname: Li
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitanaka
authors.forename: Xue-dong
authors.initials: XD
authors.lastname: Yang
mesh_terms: D000818:Animals; D032602:Caragana / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000067996:RAW 264.7 Cells
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D009569:Nitric Oxide