title: Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitro.
abstract: A major bottleneck to the therapeutic applications of dental pulp stem cells (DPSC) are their limited proliferative capacity ex vivo and tendency to undergo senescence. This may be partly due to the sub-optimal in vitro culture milieu, which could be improved by an appropriate extracellular matrix substratum. This study therefore examined decellularized matrix (DECM) from stem cells derived from human exfoliated deciduous teeth (SHED) and periodontal ligament stem cells (PDLSC), as potential substrata for DPSC culture. Both SHED-DECM and PDLSC-DECM promoted rapid adhesion and spreading of newly-seeded DPSC compared to bare polystyrene (TCPS), with vinculin immunocytochemistry showing expression of more focal adhesions by newly-adherent DPSC cultured on DECM versus TCPS. Culture of DPSC on SHED-DECM and PDLSC-DECM yielded higher proliferation of cell numbers compared to TCPS. The qRT-PCR data showed significantly higher expression of nestin by DPSC cultured on DECM versus the TCPS control. Osteogenic differentiation of DPSC was enhanced by culturing on PDLSC-DECM and SHED-DECM versus TCPS, as demonstrated by alizarin red S staining for mineralized calcium deposition, alkaline phosphatase assay and qRT-PCR analysis of key osteogenic marker expression. Hence, both SHED-DECM and PDLSC-DECM could enhance the ex vivo culture of DPSC under both non-inducing and osteogenic-inducing conditions.
authors.affiliation: Comprehensive Dental Care, Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Boon Chin
authors.initials: BC
authors.lastname: Heng
authors.affiliation: Comprehensive Dental Care, Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Shaoyue
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Comprehensive Dental Care, Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Comprehensive Dental Care, Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Changyong
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Comprehensive Dental Care, Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Ting
authors.initials: T
authors.lastname: Gong
authors.affiliation: Comprehensive Dental Care, Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong. Electronic address: zhangcf@hku.hk.
authors.forename: Chengfei
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation / Q000235:genetics*; D018920:Coculture Techniques; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000235:genetics; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism; D014094:Tooth, Deciduous / Q000166:cytology / Q000378:metabolism
keywords: Dental pulp; Extracellular matrix; Focal adhesion; Mitosis; Osteogenesis