title: Single-Cell Analysis of Growth in Budding Yeast and Bacteria Reveals a Common Size Regulation Strategy.
abstract: To maintain a constant cell size, dividing cells have to coordinate cell-cycle events with cell growth. This coordination has long been supposed to rely on the existence of size thresholds determining cell-cycle progression [1]. In budding yeast, size is controlled at the G1/S transition [2]. In agreement with this hypothesis, the size at birth influences the time spent in G1: smaller cells have a longer G1 period [3]. Nevertheless, even though cells born smaller have a longer G1, the compensation is imperfect and they still bud at smaller cell sizes. In bacteria, several recent studies have shown that the incremental model of size control, in which size is controlled by addition of a constant volume (in contrast to a size threshold), is able to quantitatively explain the experimental data on four different bacterial species [4-7]. Here, we report on experimental results for the budding yeast Saccharomyces cerevisiae, finding, surprisingly, that cell size control in this organism is very well described by the incremental model, suggesting a common strategy for cell size control with bacteria. Additionally, we argue that for S. cerevisiae the "volume increment" is not added from birth to division, but rather between two budding events.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Ilya
authors.initials: I
authors.lastname: Soifer
authors.affiliation: INRA, UMR 1319 Micalis, 78350 Jouy-en-Josas, France; AgroParisTech, UMR Micalis, 78350 Jouy-en-Josas, France; Laboratoire Jean Perrin, UPMC-CNRS UMR 8237, UPMC, 75005 Paris, France.
authors.forename: Lydia
authors.initials: L
authors.lastname: Robert
authors.affiliation: School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA. Electronic address: arielamir@seas.harvard.edu.
authors.forename: Ariel
authors.initials: A
authors.lastname: Amir
mesh_terms: D004926:Escherichia coli / Q000254:growth & development*; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D059010:Single-Cell Analysis