title: A mouse model of cytogenetic analysis to evaluate caesium137 radiation dose exposure and contamination level in lymphocytes.
abstract: In case of external overexposure to ionizing radiation, an estimation of its genotoxic effects on exposed individuals can be made retrospectively by the measurement of radiation-induced chromosome aberrations on circulating lymphocytes. Compared with external irradiation, intakes of radionuclides may, however, lead to specific features influencing dose distribution at the scale of body, of tissue or even of cell. Therefore, in case of internal contamination by radionuclides, experimental studies, particularly using animal models, are required to better understand mechanisms of their genotoxic effects and to better estimate the absorbed dose. The present study was designed to evaluate a cytogenetic method in mouse peripheral blood lymphocytes that would allow determination of yields and complexities of chromosome aberrations after low-dose rate exposure to (137)Cs delivered in vitro either by irradiation or by contamination. By using M-FISH analysis, we compared the low-dose rate responses observed in mouse to the high-dose rate responses observed both in mouse and in human. Promising similarities between the two species in the relative biological effect evaluation show that our cytogenetic model established in mouse might be useful to evaluate various radiation exposures, particularly relevant in case of intakes of radionuclides.
authors.affiliation: Laboratoire de Dosimétrie Biologique (PRP-HOM/SRBE/LDB), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 92262, Fontenay aux Roses Cedex, France. sandrine.roch-lefevre@irsn.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Roch-Lefèvre
authors.affiliation: Laboratoire de Dosimétrie Biologique (PRP-HOM/SRBE/LDB), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 92262, Fontenay aux Roses Cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Martin-Bodiot
authors.affiliation: Laboratoire de Dosimétrie Biologique (PRP-HOM/SRBE/LDB), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 92262, Fontenay aux Roses Cedex, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Grégoire
authors.affiliation: PRP-HOM/SDI, Laboratoire d'Evaluation de la Dose Interne, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 92262, Fontenay aux Roses Cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Desbrée
authors.affiliation: Laboratoire de Dosimétrie Biologique (PRP-HOM/SRBE/LDB), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 92262, Fontenay aux Roses Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Roy
authors.affiliation: Laboratoire de Dosimétrie Biologique (PRP-HOM/SRBE/LDB), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 92262, Fontenay aux Roses Cedex, France.|Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
authors.forename: Joan Francesc
authors.initials: JF
authors.lastname: Barquinero
mesh_terms: D000818:Animals; D002588:Cesium Radioisotopes / Q000009:adverse effects*; D002869:Chromosome Aberrations / Q000528:radiation effects; D020732:Cytogenetic Analysis; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D002588:Cesium Radioisotopes
keywords: Chromosome aberrations; Cs137 contamination; Low-dose rate exposure; Mouse