title: Antilisterial Activity on Poultry Meat of Amylolysin, a Bacteriocin from Bacillus amyloliquefaciens GA1.
abstract: This paper describes the production, the purification and the antilisterial activity of amylolysin, a novel bacteriocin from B. amyloliquefaciens GA1. The strain genome was first analysed using PCR techniques for the presence of gene clusters that direct the synthesis of characterised bacteriocins from B. amyloliquefaciens and the closely related B. subtilis. Our results suggest that amylolysin corresponds to a novel bacteriocin. The effect of amylolysin on the growth of different isolates of Listeria monocytogenes was evaluated in poultry meat during 21 days of storage at 4 °C. A potent antilisterial effect was observed for all the indicator strains tested, demonstrating that amylolysin is a novel bacteriocin that could be used as a food preservative.
authors.affiliation: Center for Protein Engineering (CIP), University of Liège, Bldg. B6a, Allée de la chimie, 4000, Liège, Belgium.|Walloon Center for Industrial Biology, University of Liège, Bldg. 40, Bd du Rectorat, 4000, Liège, Belgium.
authors.forename: Badre
authors.initials: B
authors.lastname: Halimi
authors.affiliation: Walloon Center for Industrial Biology, University of Liège, Bldg. 40, Bd du Rectorat, 4000, Liège, Belgium.
authors.forename: Carine
authors.initials: C
authors.lastname: Dortu
authors.affiliation: Center for Protein Engineering (CIP), University of Liège, Bldg. B6a, Allée de la chimie, 4000, Liège, Belgium.
authors.forename: Anthony
authors.initials: A
authors.lastname: Arguelles-Arias
authors.affiliation: Walloon Center for Industrial Biology, University of Liège, Bldg. 40, Bd du Rectorat, 4000, Liège, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
authors.affiliation: Center for Protein Engineering (CIP), University of Liège, Bldg. B6a, Allée de la chimie, 4000, Liège, Belgium.
authors.forename: Bernard
authors.initials: B
authors.lastname: Joris
authors.affiliation: Center for Protein Engineering (CIP), University of Liège, Bldg. B6a, Allée de la chimie, 4000, Liège, Belgium. pfickers@ulb.ac.be.|BioTechnology and BioProcess, 3Bio Department, Université Libre de Bruxelles, Av F.-D. Roosevelt, 50, CP165/61, 1050, Brussels, Belgium. pfickers@ulb.ac.be.
authors.forename: Patrick
authors.initials: P
authors.lastname: Fickers
keywords: Bacillus amyloliquefaciens; Bacteriocin; Listeria monocytogenes