title: Detection of an interaction between prion protein and neuregulin I-β1 by fluorescence resonance energy transfer analysis.
abstract: Cellular prion protein (PrP) copurifies with neuregulin type I-β1 (NRG I-β1), but no interaction has been detected by a general immunoprecipitation study. We speculate that PrP interacts with NRG I-β1. Here, the interaction of PrP with NRG I-β1 was detected by measuring fluorescence resonance energy transfer (FRET) between enhanced blue (EBFP) and enhanced green (EGFP) fluorescent protein-fusion proteins. Full-length PrP interacted with EGFP in addition to NRG I-β1. From this result, we deduced that PrP interacts with EGFP through its unstructured N-terminal domain. We therefore detected FRET between PrP deleting the N-terminal domain and NRG I-β1. In contrast, the C-terminal domain of PrP interacted with NRG I-β1 and the proteins dissociated completely in the presence of sodium chloride. This interaction occurs at the nanomolar level, which is important for the reaction to be functional in organisms. We concluded that PrP interacted with NRG I-β1 through its C-terminal domain.
authors.affiliation: a Department of Food Science and Nutrition, School of Human Environmental Sciences , Mukogawa Women's University , Nishinomiya , Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Arii
authors.affiliation: b Biological Science Course, Graduate School of Science and Engineering , Aoyama Gakuin University , Sagamihara , Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yamaguchi
authors.affiliation: c Faculty of Agriculture , Ryukoku University , Otsu , Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamasaki
authors.affiliation: b Biological Science Course, Graduate School of Science and Engineering , Aoyama Gakuin University , Sagamihara , Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Fukuoka
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D020890:Neuregulin-1 / Q000378:metabolism*; D000072002:Prion Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: C094131:NRG1 protein, human; D020890:Neuregulin-1; D000072002:Prion Proteins
keywords: coreceptor; fluorescence resonance energy transfer; interaction; neuregulin; prion protein