title: The influence of metal-based biomaterials functionalized with sphingosine-1-phosphate on the cellular response and osteogenic differentaion potenial of human adipose derived mesenchymal stem cells in vitro.
abstract: In this study, stable, homogenous and thin titania dioxide coatings (TiO2) on stainless steel substrate doped with two dosages of bioactive sphingolipids S1P were fabricated using the sol-gel method. S1P belongs to a family of sphingolipids acting as important extracellular signaling molecules and chemoattractants. This study investigated the effect of TiO2, doped with S1P in two different dosages on cellular response as well as osteogenic differentiation potential of human adipose derived multipotent stromal stem cells (hASC). The authors have shown that S1P mediates hASCs morphology, proliferation activity and population doubling time in a dose-dependent manner. They have also demonstrated that functionalization of TiO2coating with a higher dosage of S1P, i.e. 80 ng/ml [(TiO2/S1P(CII)] activated both S1PR type 1 and type 2 on mRNA level. The results indicated an increase in secretion of BMP-2, Osteopontin and Osteocalcin by osteoblasts progenitor when cultured on [TiO2/S1P(CIIm)]. In addition, the authors observed the highest extracellular matrix mineralization as well as osteonodules formation by the osteoblasts precursors when cultured onto [TiO2/S1P(CIIm)].
authors.affiliation: Department of Biology, University of Environmental and Life Sciences, Wroclaw, Poland Wrocławskie Centrum Badan EIT +, Wroclaw, Poland krzysztofmarycz@interia.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Department of Mechanics, Materials Science and Engineering, University of Technology,Wroclaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Krzak
authors.affiliation: Faculty of Veterinary Medicine, Department of Animal Physiology and Biostructure University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: Department of Anatomy, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Krzysztof A
authors.initials: KA
authors.lastname: Tomaszewski
authors.affiliation: Department of Mechanics, Materials Science and Engineering, University of Technology,Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Szczurek
authors.affiliation: Faculty of Fundamental Problems of Technology, University of Technology, Wroclaw, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Moszak
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014025:Titanium / Q000737:chemistry / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D008246:Lysophospholipids; C009495:titanium dioxide; C060506:sphingosine 1-phosphate; D014025:Titanium; D013110:Sphingosine
keywords: Sphingosine-1-phosphate; adipose derived multipotent stromal stem cells; osteogenic differentiation; sol-gel; titania dioxide coatings