title: Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination.
abstract: In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: M S P
authors.initials: MS
authors.lastname: De Azevedo
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Santos Rocha
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: V B
authors.initials: VB
authors.lastname: Pereira
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: A F
authors.initials: AF
authors.lastname: De Oliveira Junior
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: C S
authors.initials: CS
authors.lastname: De Sousa
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: CERELA-CONICET, Chacabuco 145, Tucuman, Argentina.
authors.forename: J G
authors.initials: JG
authors.lastname: LeBlanc
authors.affiliation: Institut National de la Recherche Agronomique, MICALIS (UMR 1319), Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Chatel
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Miyoshi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics* / Q000276:immunology*; D001428:Bacterial Vaccines; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology*; D006460:Hemolysin Proteins / Q000235:genetics* / Q000276:immunology*; D006461:Hemolysis; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000276:immunology*; D009154:Mutation; D011994:Recombinant Proteins; D014611:Vaccination
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C038705:hlyA protein, Listeria monocytogenes