title: Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation.
abstract: Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3(.)DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on (1)H- and (13)C NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation.
authors.affiliation: CBMR-Centre for Biomedical Research, Faculty of Sciences and Technology, University of Algarve, 8005-139 Faro, Portugal. Electronic address: maritadionisio@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Dionísio
authors.affiliation: CIQA-Algarve Chemistry Research Centre, University of Algarve, 8005-139 Faro, Portugal; School of Health-University of Algarve, 8000-510 Faro, Portugal; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. Electronic address: lvbraz@ualg.pt.
authors.forename: L
authors.initials: L
authors.lastname: Braz
authors.affiliation: UCIBIO, REQUIMTE, Department of Chemistry, and CENIMAT/I3N, Department of Materials Science, Faculty of Science and Technology, UNL, 2829-516 Caparica, Portugal. Electronic address: marta.corvo@fct.unl.pt.
authors.forename: M
authors.initials: M
authors.lastname: Corvo
authors.affiliation: CIQA-Algarve Chemistry Research Centre, University of Algarve, 8005-139 Faro, Portugal; Department of Chemistry and Pharmacy, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal; CQE-Centro de Química Estrutural, Instituto Superior Técnico, University of Lisbon, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: jlouren@ualg.pt.
authors.forename: J P
authors.initials: JP
authors.lastname: Lourenço
authors.affiliation: CBMR-Centre for Biomedical Research, Faculty of Sciences and Technology, University of Algarve, 8005-139 Faro, Portugal; Department of Chemistry and Pharmacy, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal. Electronic address: amgrenha@ualg.pt.
authors.forename: A
authors.initials: A
authors.lastname: Grenha
authors.affiliation: CIQA-Algarve Chemistry Research Centre, University of Algarve, 8005-139 Faro, Portugal; Department of Chemistry and Pharmacy, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal. Electronic address: amcosta@ualg.pt.
authors.forename: A M
authors.initials: AM
authors.lastname: Rosa da Costa
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D004355:Drug Stability; D005936:Glucans / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006860:Hydrogen Bonding; D009113:Muramidase / Q000378:metabolism; D053758:Nanoparticles; D000071228:Polyelectrolytes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D055672:Static Electricity; D013431:Sulfates / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D005936:Glucans; D000071228:Polyelectrolytes; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D012710:Serum Albumin, Bovine; C009109:pullulan; D009113:Muramidase
keywords: (3–6): Sulfated pullulan; Polyelectrolyte complexation; Tetraalkylammonium pullulan