title: Oestrogen activity of the serum in adolescents with Type 1 diabetes.
abstract: To assess serum oestrogen levels and oestrogenic activity in adolescents with Type 1 diabetes compared with a healthy control group.
  
  
  
  We conducted a cross-sectional study that evaluated adolescents with Type 1 diabetes (n = 38) and healthy adolescents (control group; n = 32). Serum oestrogens, urinary oestrogen metabolites and serum oestrogenic activity were assessed. Oestrogenic activity was evaluated in an in vitro cell proliferation assay using a modified E-screen assay with MCF-7/BUS cells.
  
  
  
  Adolescents with Type 1 diabetes had lower oestrogenic activity levels in both phases of the menstrual cycle compared with the control group (follicular phase: 76 vs 94%; luteal phase: 97 vs 131%; P < 0.01), even after adjusting for BMI, oestradiol and oestrone levels. Postmenarcheal adolescents with Type 1 diabetes had lower oestradiol levels compared with control subjects in the follicular phase (63.3 pmol/l vs 89.4 pmol/l; P < 0.01) and higher oestrone levels compared with controls in the luteal phase (196 vs 151.9 pmol/l; P < 0.05).
  
  
  
  Adolescents with Type 1 diabetes had lower levels of serum oestrogenic activity, and these were lower than expected based on their serum oestradiol levels. We postulate that changes in the serum milieu of oestrogens in patients with Type 1 diabetes may explain their decreased oestrogenic activity and may play a role in their adverse metabolic profile.
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.
authors.forename: D
authors.initials: D
authors.lastname: Martínez
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.
authors.forename: A
authors.initials: A
authors.lastname: Castro
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.
authors.forename: P M
authors.initials: PM
authors.lastname: Merino
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.|Hospital San Borja Arriarán, Servicio Salud Metropolitano Central, Santiago, Chile.
authors.forename: P
authors.initials: P
authors.lastname: López
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.
authors.forename: M C
authors.initials: MC
authors.lastname: Lardone
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.
authors.forename: G
authors.initials: G
authors.lastname: Iñiguez
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile.
authors.forename: F
authors.initials: F
authors.lastname: Cassorla
authors.affiliation: Faculty of Medicine, Institute of Maternal and Child Research, University of Chile. ecodner@med.uchile.cl.
authors.forename: E
authors.initials: E
authors.lastname: Codner
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002478:Cells, Cultured; D002648:Child; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008597:Menstrual Cycle / Q000097:blood / Q000502:physiology
chemical_list: D004958:Estradiol