title: Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules.
abstract: In the present report, we are making the proof of concept of cell small populations (from 1 to 100 cells) spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume (30 nL), directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging (SPRi) experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen (PSA) and β-2-microglobulin (B2M) secreted by prostate cancer cells following induction by dihydrotestosterone (DHT). Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip.
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires-Institut de Chimie et Biochimie Moléculaires et Supramoléculaires-Université Claude Bernard Lyon 1, Villeurbanne 69100, France. berthuy@scienion.de.
authors.forename: Ophélie I
authors.initials: OI
authors.lastname: Berthuy
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires-Institut de Chimie et Biochimie Moléculaires et Supramoléculaires-Université Claude Bernard Lyon 1, Villeurbanne 69100, France. loic.blum@univ-lyon1.fr.
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.affiliation: Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires-Institut de Chimie et Biochimie Moléculaires et Supramoléculaires-Université Claude Bernard Lyon 1, Villeurbanne 69100, France. christophe.marquette@univ-lyon1.fr.
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D013196:Dihydrotestosterone / Q000494:pharmacology; D006046:Gold / Q000737:chemistry; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods; D001613:beta 2-Microglobulin / Q000378:metabolism*
chemical_list: D001613:beta 2-Microglobulin; D013196:Dihydrotestosterone; D006046:Gold; D017430:Prostate-Specific Antigen
keywords: SPRi; biosensor; cell encapsulation; cell microarray; real-time