title: MicroRNA-455 suppresses non-small cell lung cancer through targeting ZEB1.
abstract: MicroRNA-455 (miRNA-455), which is downregulated in human cancer, potently mediates the multiple steps of carcinogenesis. However, the role of miR-455 in non-small cell lung cancer (NSCLC) carcinogenesis remains unclear. In present study, we determined the mature miRNA-455 expression in NSCLC tissues and cells by real-time PCR. Follow-up studies examined the effects of a miR-455 mimic (gain of function) on cell proliferation, migration, and invasion. Our data indicate that miR-455 was significantly down-regulated in NSCLC cell lines and tissues. In functional assays, overexpression of miR-455 suppressed the proliferation, migration, and invasion of NSCLC cell lines. Data from reporter assays showed that miR-455 directly binds to 3'UTR of ZEB1 and suppresses the endogenous level of ZEB1 protein expression. Furthermore, overexpression of ZEB1 reverses miR-455-suppressed malignant phenotype of NSCLC cells. Moreover, we found that upregulation of ZEB1 expression is inversely associated with miR-455 expression in NSCLC tissues. Taken together, miR-455 as an anti-oncogene in non-small cell lung cancer through up-regulation of ZEB1 and serve as a potential therapeutic target in NSCLC.
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Chinese PLA General Hospital, Beijing, 100048, China.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Chinese PLA General Hospital, Beijing, 100048, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ping
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Chinese PLA General Hospital, Beijing, 100048, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Chinese PLA General Hospital, Beijing, 100048, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D015854:Up-Regulation; D000071799:Zinc Finger E-box-Binding Homeobox 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C583404:MIRN455 microRNA, human; D035683:MicroRNAs; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1
keywords: ZEB1; miR-455; migration; non-small cell lung cancer