title: Mitochondrial dependent oxidative stress in cell culture induced by laser radiation at 1265 nm.
abstract: Photodynamic therapy is the main technique applied for surface carcinoma treatment. This technique employs singlet oxygen generated via a laser excited photosensitizer as a main damaging agent. However, prolonged sensitivity to intensive light, relatively low tissue penetration by activating light the cost of photosensitizer (PS) administration can limit photodynamic therapy applications. Early was reported singlet oxygen generation without photosensitizer induced by a laser irradiation at the wavelength of 1250-1270 nm. Here, we study the dynamics of oxidative stress, DNA damage, changes of mitochondrial potential, and mitochondrial mass induced by a laser at 1265 nm have been studied in HCT-116 and CHO-K cells. Laser irradiation of HCT-116 and CHO-K cells has induced a dose-dependent cell death via increasing intracellular reactive oxygen species (ROS) concentration, increase of DNA damage, decrease of mitochondrial potential, and reduced glutathione. It has been shown that, along with singlet oxygen generation, the increase of the intracellular ROS concentration induced by mitochondrial damage contributes to the damaging effect of the laser irradiation at 1265 nm.
authors.affiliation: Laboratory of Molecular and Cell Biology, S.P. Kapitsa Technological Research Institute, Ulyanovsk State University, Lva Tolstogo 42, Ulyanovsk, 432017, Russia Federation. saenkoyv@yandex.ru.
authors.forename: Yury V
authors.initials: YV
authors.lastname: Saenko
authors.affiliation: Laboratory of Molecular and Cell Biology, S.P. Kapitsa Technological Research Institute, Ulyanovsk State University, Lva Tolstogo 42, Ulyanovsk, 432017, Russia Federation.
authors.forename: Eugenia S
authors.initials: ES
authors.lastname: Glushchenko
authors.affiliation: Laboratory of Quantum Electronics and Optoelectronics, S.P. Kapitsa Technological Research Institute, Ulyanovsk State University, Lva Tolstogo 42, Ulyanovsk, 432017, Russia Federation.
authors.forename: Igor O
authors.initials: IO
authors.lastname: Zolotovskii
authors.affiliation: A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Vavilov Str. 38, Moscow, 119991, Russia Federation.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Sholokhov
authors.affiliation: A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Vavilov Str. 38, Moscow, 119991, Russia Federation.
authors.forename: Andrey
authors.initials: A
authors.lastname: Kurkov
mesh_terms: D000818:Animals; D016466:CHO Cells; D016923:Cell Death; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D007834:Lasers; D028022:Low-Level Light Therapy / Q000009:adverse effects; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism* / Q000528:radiation effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010778:Photochemotherapy / Q000009:adverse effects*; D026082:Singlet Oxygen / Q000378:metabolism
chemical_list: D026082:Singlet Oxygen; D005978:Glutathione
keywords: 1265 nm Raman laser; DNA damage; Mitochondrial potential; Oxidative stress; Reactive oxygen species