title: Preparation and in vitro evaluation of 5-flourouracil loaded magnetite-zeolite nanocomposite (5-FU-MZNC) for cancer drug delivery applications.
abstract: In this work, super paramagnetic magnetite nanoparticles were synthesized onto/into zeolite, then loaded with anti-cancer drug 5-fluorouracil (5-FU). The physical properties of the prepared nanocomposite and drug loaded nanocomposite were characterized using different techniques. The drug loading and releasing behavior of the magnetic nanocarrier was investigated and the drug-loaded nanoparticles exhibited a sustained release of drug without any burst release phenomenon. Furthermore, 5-FU loaded MZNC were evaluated for its biological characteristics. The functional 5-FU-MZNC has been triggered intra-cellular release of the cancer therapeutic agent 5-fluorouracil (5-FU). Cytotoxic effects of 5-FU loaded MZNC on human gastric carcinoma (AGS) cells were determined by real time cell analysis and colorimetric WST-1 cell viability assay. Apoptosis of cells was further investigated by Annexin-V staining which indicates the loss of cell membrane integrity. According to our results, 5-FU-MZNC showed a concentration-dependent cell proliferation inhibitory function against AGS cells. Morphologic and apoptotic images were consistent with the cytotoxicity results. In conclusion, 5-FU loaded MZNC efficiently inhibit the proliferation of AGS cells in vitro through apoptotic mechanisms, and may be a beneficial agent against cancer, however further animal study is still required.
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Tuğba
authors.initials: T
authors.lastname: Sağir
authors.affiliation: Institute of Biomedical Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Huysal
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Bezmialem Vakıf University, Fatih, 34093 Istanbul, Turkey.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Durmus
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bezmialem Vakıf University, Fatih, 34093 Istanbul, Turkey.
authors.forename: Belma Zengin
authors.initials: BZ
authors.lastname: Kurt
authors.affiliation: Institute of Biomedical Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey. Electronic address: msenel@fatih.edu.tr.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Senel
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey. Electronic address: isiksevim@fatih.edu.tr.
authors.forename: Sevim
authors.initials: S
authors.lastname: Isık
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003692:Delayed-Action Preparations; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D065546:Drug Liberation; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005472:Fluorouracil / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D017641:Zeolites / Q000737:chemistry*
chemical_list: D000964:Antimetabolites, Antineoplastic; D003692:Delayed-Action Preparations; D017641:Zeolites; D005472:Fluorouracil; D052203:Ferrosoferric Oxide
keywords: 5-Flourouracil; Cancer; In vitro study; Magnetite; Nanoparticle; Zeolite