title: DNA barcoding revealed Nematodospora valgi gen. nov., sp. nov. and Candida cetoniae sp. nov. in the Lodderomyces clade.
abstract: During a yeast biodiversity survey conducted in 2009-2011 in Bulgaria (South Eastern Europe) five strains of a novel ascomycetous yeast species were isolated from the beetle Valgus hemipterus (Cetoniinae) collected from two localities, namely Osogovska Planina Mountain and Nature Park Zlatni Pyasatsi. Phylogenetic analysis using combined sequences of the D1/D2 domains of the large subunit ribosomal DNA (LSU rDNA) and the internal transcribed spacers 1 + 2 regions (ITS1+2) placed the novel species on a separate branch near the basal part of the Lodderomyces clade. The novel species has a unique ascospore morphology distinct from those of the closely related teleomorphic genus Lodderomyces. Based on phylogenetic analysis and morphology of the ascospores we propose Nematodospora valgi gen. nov., sp. nov. to accommodate these isolates (MB811804 D37S(T), MB802458). Two strains of a novel anamorphic yeast species were isolated from the beetles Cetonia aurata and Oxythyrea funesta (Cetoniinae) collected in East Rhodopies and Sofia city, respectively. DNA barcoding analysis placed the new yeast species within the Candida parapsilosis subclade. Here, we present the description of a new yeast species, Candida cetoniae sp. nov. (IMB1R2(T), MB803501) to accommodate these two strains. The ecology and biogeography of the insect-associated yeasts of the Lodderomyces clade is discussed.
authors.affiliation: The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev, Sofia 1113, Bulgaria. Electronic address: dilnorag@gmail.com.
authors.forename: Dilnora E
authors.initials: DE
authors.lastname: Gouliamova
authors.affiliation: Sofia University "St. Kliment Ohridski", 5 James Bourchier Blvd., Sofia 1164, Bulgaria.
authors.forename: Roumen A
authors.initials: RA
authors.lastname: Dimitrov
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Maudy Th
authors.initials: MT
authors.lastname: Smith
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Marizeth
authors.initials: M
authors.lastname: Groenewald
authors.affiliation: The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev, Sofia 1113, Bulgaria.
authors.forename: Margarita M
authors.initials: MM
authors.lastname: Stoilova-Disheva
authors.affiliation: National Museum of Natural History, 1 Tsar Osvoboditel Blvd., Sofia 1000, Bulgaria.
authors.forename: Borislav V
authors.initials: BV
authors.lastname: Guéorguiev
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Shanghai Key Laboratory of Molecular Medical Mycology, Second Military Medical University, Changzheng Hospital, Institute of Dermatology and Medical Mycology, Shanghai 200003, China.
authors.forename: Teun
authors.initials: T
authors.lastname: Boekhout
mesh_terms: D000818:Animals; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001517:Coleoptera / Q000382:microbiology*; D058893:DNA Barcoding, Taxonomic; D004271:DNA, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal
keywords: Biodiversity; Biogeography; Insect associated yeasts; Pathogens