title: Redox proteomics for the assessment of redox-related posttranslational regulation in plants.
abstract: The methodological developments of in vivo and in vitro protein labeling and subsequent detection enable sensitive and specific detection of redox modifications. Such methods are presently applied to diverse cells and tissues, subproteomes and developmental as well as environmental conditions. The chloroplast proteome is particularly suitable for such kind of studies, because redox regulation of chloroplast proteins is well established, many plastid proteins are abundant, redox network components have been inventoried in great depth, and functional consequences explored. Thus the repertoire of redox-related posttranslational modifications on the one hand side and their abundance on the other pose a challenge for the near future to understand their contribution to physiological regulation. The various posttranslational redox modifications are introduced, followed by a description of the available proteomics methods. The significance of the redox-related posttranslational modification is exemplarily worked out using established examples from photosynthesis. This article is part of a Special Issue entitled: Plant Proteomics--a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock.
authors.affiliation: Applied Biochemistry, Institute of Plant Genetics and Crop Plant Research, IPK, Corrensstrasse 3, 06466 Gatersleben, Germany. Electronic address: mock@ipk-gatersleben.de.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
authors.affiliation: Biochemistry and Physiology of Plants, Faculty of Biology - W5-134, Bielefeld University, 33501 Bielefeld, Germany. Electronic address: karl-josef.dietz@uni-bielefeld.de.
authors.forename: Karl-Josef
authors.initials: KJ
authors.lastname: Dietz
mesh_terms: D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D010084:Oxidation-Reduction; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D060365:Chloroplast Proteins; D020543:Proteome
keywords: Chloroplast; Molecular reactive species; Photosynthesis; Post-translational modification; Redox proteomics; Regulation