title: Concentration and stabilization of C₂₀-₂₂ n-3 polyunsaturated fatty acid esters from the oil of Sardinella longiceps.
abstract: Methyl esters of C20-22n-3 polyunsaturated fatty acids derived from sardine oil triglycerides were concentrated to 86% purity with greater than 30% recovery by argentated chromatography. The synergistic effect of ethyl acetate fractions of seaweeds Kappaphycus alvarezii, Hypnea musciformis and Jania rubens used in 0.1:0.2:0.2 (%, w/w) ratio in arresting oxidative degradation of the n-3 PUFA methyl ester concentrate was demonstrated during accelerated storage. The induction time (6.8h) and antioxidant activity indices (>24) were greater for n-3 PUFA concentrates supplemented with seaweed extracts than antioxidants BHT and α-tocopherol (<5h and <17, respectively). Nuclear Magnetic Resonance spectroscopy was employed to study the oxidative changes of fatty acid signals of PUFA concentrate during accelerated storage. Potential of seaweeds to improve the storage stability of C20-22n-3 fatty acid methyl esters was studied. This study has applications in development of food and pharmaceutical products.
authors.affiliation: Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India. Electronic address: kajal_cmfri@yahoo.com.
authors.forename: Kajal
authors.initials: K
authors.lastname: Chakraborty
authors.affiliation: Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.
authors.forename: Deepu
authors.initials: D
authors.lastname: Joseph
authors.affiliation: Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.
authors.forename: Dexy
authors.initials: D
authors.lastname: Joseph
mesh_terms: D000818:Animals; D004952:Esters; D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy
chemical_list: D004952:Esters; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils
keywords: Argentated chromatography; C(20-22)n-3 polyunsaturated fatty acids; Nuclear Magnetic Resonance spectroscopy; Oxidative stability; Sardine oil; Seaweeds