title: Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma.
abstract: Dysregulation of microRNAs (miRs) is associated with cancer development and progression and aberrant expression of miR-874 have been found in some types of cancer. However, the expression and function of miR-874 in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-874 in osteosarcoma tumorigenesis and development. The expression level of miR-874 was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) in human osteosarcoma cell lines and tissues. Using a miR-874 mimic, cell proliferation and migration assays were performed in an osteosarcoma cell line and tumorigenicity was observed in vivo in order to determine the effects of miR-874 in osteosarcoma cell lines and tissues. MiR-874 was significantly downregulated in osteosarcoma cell lines and clinical specimens. Decreased miR-874 expression was significantly associated with large tumor size, distant metastasis, and advanced clinical stage, and was an independent predictor of poor survival. Overexpression of miR-874 inhibited cell proliferation, invasion and migration in vitro, promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. These findings indicate that miR-874 may act as a tumor suppressor in osteosarcoma and could serve as a novel therapeutic agent for miR-based therapy.
authors.affiliation: Department of Orthopedics, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, The People's Hospital of Linqu, Weifang, Shandong, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Li
authors.affiliation: Department of Surgery, Health Center in Sitou Town, Weifang, Shandong, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Department of Oncology, Qingzhou Chinese Medicine Hospital, Qingzhou, Shandong, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, The People's Hospital of Weifang, Shandong, China.
authors.forename: Q S
authors.initials: QS
authors.lastname: Zhuang
authors.affiliation: Department of Orthopedics, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Fang