title: [Alleviation of salt stress during maize seed germination by presoaking with exogenous sugar].
abstract: The maize variety Kenyu 6 was used to study the effects of exogenous glucose (Glc) and sucrose (Suc) on salt tolerance of maize seeds at germination stage under 150 mmol · L(-1) NaCl treatment. Results showed that under salt stress condition, 0.5 mmol · L(-1) exogenous Glc and Suc presoaking could promote seed germination and early seedling growth. Compared with the salt treatment, Glc presoaking increased the shoot length, radicle length and corresponding dry mass up to 1.5, 1.3, 2.1 and 1.8 times, and those of the Suc presoaking treatment increased up to 1.7, 1.3. 2.7 and 1.9 times, respectively. Exogenous Glc and Suc presoaking resulted in decreased levels of thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) content of maize shoot under salt stress, which were lowered by 24.9% and 20.6% respectively. Exogenous Glc and Suc presoaking could increase the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione peroxidase (GPX), glutathione reductase (GR) and induce glucose-6-phosphate dehydrogenase (G6PDH) activity of maize shoot under salt stress. Compared with the salt treatment. Glc presoaking increased the activity of SOD, APX, GPX, GR and G6PDH by 66.2%, 62.9%, 32.0%, 38.5% and 50.5%, and those of the Suc presoaking increased by 67.5%, 59.8%, 30.0%, 38.5% and 50.4%, respectively. Glc and Suc presoaking also significantly increased the contents of ascorbic acid (ASA) and glutathione (GSH), ASA/DHA and GSH/GSSG. The G6PDH activity was found closely related with the strong antioxidation capacity induced by exogenous sugars. In addition, Glc and Suc presoaking enhanced K+/Na+ in maize shoot by 1.3 and 1.4 times of water soaking salt treatment, respectively. These results indicated that exogenous Glc and Suc presoaking could improve antioxidation capacity of maize seeds and maintain the in vivo K+/Na+ ion balance to alleviate the inhibitory effect of salt stress on maize seed germination.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ke-jun
authors.initials: KJ
authors.lastname: Yang
authors.forename: Zuo-tong
authors.initials: ZT
authors.lastname: Li
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Zhao
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Xu
authors.forename: Xue- wei
authors.initials: X
authors.lastname: Hu
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Shi
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Ma
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D060387:Ascorbate Peroxidases / Q000737:chemistry; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D004281:Docosahexaenoic Acids / Q000737:chemistry; D018525:Germination; D005947:Glucose / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000737:chemistry; D005980:Glutathione Reductase / Q000737:chemistry; D006861:Hydrogen Peroxide; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development*; D012965:Sodium Chloride / Q000737:chemistry*; D013395:Sucrose / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000737:chemistry; D003313:Zea mays / Q000254:growth & development*
chemical_list: D000975:Antioxidants; C111716:docosahexaenoylascorbic acid; D004281:Docosahexaenoic Acids; D012965:Sodium Chloride; D013395:Sucrose; D006861:Hydrogen Peroxide; D005954:Glucosephosphate Dehydrogenase; D060387:Ascorbate Peroxidases; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D005947:Glucose; D001205:Ascorbic Acid