title: Purification and characterization of a soluble glycoprotein from garlic (Allium sativum) and its in vitro bioactivity.
abstract: A soluble glycoprotein was purified to homogeneity from ripe garlic (Allium sativum) bulbs using ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl-52 cellulose anion-exchange chromatography. A native mass of 55.7 kDa estimated on gel permeation chromatography and a molecular weight of 13.2 kDa observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis supported that the glycoprotein is a homotetramer. β-Elimination reaction result suggested that the glycoprotein is an N-linked type. Fourier-transform infrared spectroscopy proved that it contains sugar. Gas chromatography-mass spectrometer analysis showed that its sugar component was galactose. The glycoprotein has 1,1-diphenyl-2-picrylhydrazil free radical scavenging activity and the peroxidation inhibition ability to polyunsaturated fatty acid. These results indicated that the glycoprotein has potential for food additives, functional foods, and even biotechnological and medical applications.
authors.affiliation: a College of Bioscience and Biotechnology , Hunan Agricultural University , Changsha , China.|b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.|c Collaborative Innovation Center for Farmland Weeds Control , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zou
authors.affiliation: d College of Medicine , University of Florida , Jacksonville , Florida , USA.
authors.forename: Minghui
authors.initials: M
authors.lastname: Xiang
authors.affiliation: b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.|c Collaborative Innovation Center for Farmland Weeds Control , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Chenzhong
authors.initials: C
authors.lastname: Jin
authors.affiliation: b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.|c Collaborative Innovation Center for Farmland Weeds Control , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Qiuqing
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: b Department of Life Sciences , Hunan University of Humanities, Science and Technology , Loudi , China.|c Collaborative Innovation Center for Farmland Weeds Control , Hunan University of Humanities, Science and Technology , Loudi , China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Hu
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005737:Garlic / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D066298:In Vitro Techniques; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006023:Glycoproteins
keywords: Garlic; glycoprotein; homotetramer; in vitro bioactivity; purification