title: IMPACT OF CYCLICAL CHANGES IN TEMPERATURE ON CIRCADIAN CLOCK GENES EXPRESSION IN Bombyx BmN CELLS.
abstract: The physiology and metabolism of poikilothermic insects are under the control of environmental temperature. Temperature is the primary cue for the circadian rhythm. Reports on the timing mechanisms of temperature in lepidopterans are limited. This study used Bombyx mori BmN ovarian cells to investigate the effect of temperature on expression of the main circadian clock genes in a negative feedback loop. A 37°C, 30-min high-temperature stimulation induced transcription of the circadian clock genes Cry1, Cry2, Per, and Tim. The gene expression profiles showed rhythmic oscillations, with shortened oscillatory periods for Cry1 and altered oscillatory phases for Cry1 and Per. Cyclical increases in temperature of 2°C starting at 26°C, 5°C starting at 20 or 25°C, or 10°C starting at 20°C induced cyclical changes in expression and protein from the four circadian clock genes. Cyclical temperature changes with a difference of 10°C had the most influence. In conclusion, cyclical changes in temperature with differences from 2 to 10°C reset and synchronized the circadian clock of silkworm BmN cells. Transcription of the genes for and protein from Cry2 and Per showed a better reset and synchronization with cyclical temperature changes.
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.|Institute of Agricultural Biotechnology & Ecology (IABE), Soochow University, Suzhou, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Qiu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.|Institute of Agricultural Biotechnology & Ecology (IABE), Soochow University, Suzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tao
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.|Institute of Agricultural Biotechnology & Ecology (IABE), Soochow University, Suzhou, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Shu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.
authors.forename: Heng-Jiang
authors.initials: HJ
authors.lastname: Liu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.|Institute of Agricultural Biotechnology & Ecology (IABE), Soochow University, Suzhou, China.|National Engineering Laboratory for Modern Silk (NEAER), Soochow University, Suzhou, China.
authors.forename: Yanghu
authors.initials: Y
authors.lastname: SiMa
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, China.|Institute of Agricultural Biotechnology & Ecology (IABE), Soochow University, Suzhou, China.|National Engineering Laboratory for Modern Silk (NEAER), Soochow University, Suzhou, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002940:Circadian Rhythm; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D056925:Circadian Rhythm Signaling Peptides and Proteins; D019476:Insect Proteins
keywords: BmN cells; Bombyx mori; Circadian rhythm; circadian clock genes; temperature