title: Over-expression of CYP6A2 is associated with spirotetramat resistance and cross-resistance in the resistant strain of Aphis gossypii Glover.
abstract: A laboratory-selected spirotetramat-resistant strain (SR) of cotton aphid developed 579-fold and 15-fold resistance to spirotetramat in adult aphids and 3rd instar nymphs, respectively, compared with a susceptible strain (SS) [26]. The SR strain developed high-level cross-resistance to alpha-cypermethrin and bifenthrin and very low or no cross-resistance to the other tested insecticides. Synergist piperonyl butoxide (PBO) dramatically increased the toxicity of spirotetramat and alpha-cypermethrin in the resistant strain. RT-qPCR results demonstrated that the transcriptional levels of CYP6A2 increased significantly in the SR strain compared with the SS strain, which was consistent with the transcriptome results [30]. The depletion of CYP6A2 transcripts by RNAi also significantly increased the sensitivity of the resistant aphid to spirotetramat and alpha-cypermethrin. These results indicate the possible involvement of CYP6A2 in spirotetramat resistance and alpha-cypermethrin cross-resistance in the cotton aphid. These together with other cross-resistance results have implications for the successful implementation of resistance management strategies for Aphis gossypii.
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Tianfei
authors.initials: T
authors.lastname: Peng
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Yiou
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xi
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: E
authors.initials: E
authors.lastname: Zhu
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Xin
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhan
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China. Electronic address: shangqingli@163.com.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Shang
mesh_terms: D000818:Animals; D001042:Aphids / Q000187:drug effects* / Q000201:enzymology; D001372:Aza Compounds / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000633:toxicity*; D010574:Pesticide Synergists / Q000633:toxicity; D010882:Piperonyl Butoxide / Q000633:toxicity; D011722:Pyrethrins / Q000633:toxicity; D034622:RNA Interference; D013141:Spiro Compounds / Q000633:toxicity*
chemical_list: D001372:Aza Compounds; D019476:Insect Proteins; D007306:Insecticides; D010574:Pesticide Synergists; D011722:Pyrethrins; D013141:Spiro Compounds; C017160:cypermethrin; C570705:spirotetramat; D003577:Cytochrome P-450 Enzyme System; D010882:Piperonyl Butoxide
keywords: Aphis gossypii; Cytochrome P450 monooxygenases; Resistance; Spirotetramat