title: TaOPR2 encodes a 12-oxo-phytodienoic acid reductase involved in the biosynthesis of jasmonic acid in wheat (Triticum aestivum L.).
abstract: The 12-oxo-phytodienoic acid reductases (OPRs) are involved in the various processes of growth and development in plants, and classified into the OPRⅠ and OPRⅡ subgroups. In higher plants, only OPRⅡ subgroup genes take part in the biosynthesis of endogenous jasmonic acid. In this study, we isolated a novel OPRⅡ subgroup gene named TaOPR2 (GeneBank accession: KM216389) from the thermo-sensitive genic male sterile (TGMS) wheat cultivar BS366. TaOPR2 was predicted to encode a protein with 390 amino acids. The encoded protein contained the typical oxidored_FMN domain, the C-terminus peroxisomal-targeting signal peptide, and conserved FMN-binding sites. TaOPR2 was mapped to wheat chromosome 7B and located on peroxisome. Protein evolution analysis revealed that TaOPR2 belongs to the OPRⅡ subgroup and shares a high degree of identity with other higher plant OPR proteins. The quantitative real-time PCR results indicated that the expression of TaOPR2 is inhibited by abscisic acid (ABA), salicylic acid (SA), gibberellic acid (GA3), low temperatures and high salinity. In contrast, the expression of TaOPR2 can be induced by wounding, drought and methyl jasmonate (MeJA). Furthermore, the transcription level of TaOPR2 increased after infection with Puccinia striiformis f. sp. tritici and Puccinia recondite f. sp. tritici. TaOPR2 has NADPH-dependent oxidoreductase activity. In addition, the constitutive expression of TaOPR2 can rescue the male sterility phenotype of Arabidopsis mutant opr3. These results suggest that TaOPR2 is involved in the biosynthesis of jasmonic acid (JA) in wheat.
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China; College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Duan
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China; College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Bai
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Fengting
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Gao
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China. Electronic address: lpzhang8@126.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing 100097, China.
authors.forename: Changping
authors.initials: C
authors.lastname: Zhao
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D004789:Enzyme Activation; D009249:NADP / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D051479:Plant Infertility / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D009249:NADP; C011006:jasmonic acid; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase
keywords: 12-oxo-phytodienoic acid reductase; Biosynthesis; Jasmonic acid; Wheat