title: Effects of caffeine supplementation in post-thaw human semen over different incubation periods.
abstract: This study aimed to evaluate the effects of caffeine supplementation in post-cryopreservation human semen over different incubation periods. After collection by masturbation, 17 semen samples were analysed according to World Health Organization criteria, processed and cryopreserved with TEST-yolk buffer (1 : 1) in liquid nitrogen. After a thawing protocol, samples were incubated with 2 mm of caffeine for 0, 5, 15, 30 or 60 min, followed by analysis of motility and mitochondrial activity using 3,3'-diaminobenzidine (DAB). Mean variance analysis was performed, and P < 0.05 was the adopted significance threshold. Samples incubated for 15 min showed increased progressive motility compared to other periods of incubation, as well as a reduced percentage of immotile spermatozoa (P < 0.05). In samples incubated for 5 min, increased mitochondrial activity above 50% was observed (DABI and DABII). Although cryosurvival rates were low after the cryopreservation process, incubation with caffeine was associated with an increase in sperm motility, particularly 15-min incubation, suggesting that incubation with caffeine can be an important tool in patients with worsening seminal quality undergoing infertility treatment.
authors.affiliation: Androscience - High Complexity Clinical and Research Andrology Laboratory, São Paulo, Brazil. juliana_rp@hotmail.com, lab@androscience.com.br.|Sector of Andrology, Department of Urology, Universidade de São Paulo, São Paulo, Brazil. juliana_rp@hotmail.com, lab@androscience.com.br.|Unit of Reproductive Toxicology, Department of Pathology, Universidade de São Paulo, São Paulo, Brazil. juliana_rp@hotmail.com, lab@androscience.com.br.
authors.forename: J R
authors.initials: JR
authors.lastname: Pariz
authors.affiliation: Androscience - High Complexity Clinical and Research Andrology Laboratory, São Paulo, Brazil.|Sector of Andrology, Department of Urology, Universidade de São Paulo, São Paulo, Brazil.|Unit of Reproductive Toxicology, Department of Pathology, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Hallak
mesh_terms: D000328:Adult; D002110:Caffeine / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012661:Semen; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D002110:Caffeine
keywords: 
  spermatozoa
  ; Caffeine; cryopreservation; mitochondria; sperm motility