title: Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing.
abstract: Aberrant sensing of self-nucleic acids by Toll-like receptor (TLR) 7, 8, or 9 is associated with several autoimmune disorders, including systemic lupus erythematosus (SLE), rheumatoid arthritis, psoriasis, or systemic sclerosis. In recent years, several classes of synthetic oligonucleotides have been shown to antagonize sensing of immunostimulatory nucleic acids by TLR7/8/9, indicating that these molecules could have therapeutic applications in such autoimmune diseases. Conversely, synthetic oligonucleotides used in therapeutic technologies such as antisense and microRNA inhibitors also have the potential to inhibit TLR7/8/9 sensing, rendering patients more susceptible to viral/bacterial infections. This chapter describes a protocol to define the inhibitory activity of synthetic oligonucleotides on TLR7.
authors.affiliation: Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton, VIC, 3168, Australia.|Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Ferrand
authors.affiliation: Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton, VIC, 3168, Australia. michael.gantier@hudson.org.au.|Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia. michael.gantier@hudson.org.au.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Gantier
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D008024:Ligands; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D011485:Protein Binding; D012313:RNA / Q000235:genetics / Q000276:immunology; D051199:Toll-Like Receptor 7 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D008024:Ligands; D009841:Oligonucleotides; D051199:Toll-Like Receptor 7; D014409:Tumor Necrosis Factor-alpha; D012313:RNA
keywords: Innate immunity; Oligonucleotides; TLR7; TLR8; Toll-like receptors