title: Thrombin-Responsive Gated Silica Mesoporous Nanoparticles As Coagulation Regulators.
abstract: The possibility of achieving sophisticated actions in complex biological environments using gated nanoparticles is an exciting prospect with much potential. We herein describe new gated mesoporous silica nanoparticles (MSN) loaded with an anticoagulant drug and capped with a peptide containing a thrombin-specific cleavage site. When the coagulation cascade was triggered, active thrombin degraded the capping peptidic sequence and induced the release of anticoagulant drugs to delay the clotting process. The thrombin-dependent response was assessed and a significant increase in coagulation time in plasma from 2.6 min to 5 min was found. This work broadens the application of gated silica nanoparticles and demonstrates their ability to act as controllers in a complex scenario such as hemostasis.
authors.affiliation: Materials Chemistry Laboratory, Department of Materials Science and Department of Chemistry, Gulbarga University , Gulbarga-585106, India.
authors.forename: Ravishankar
authors.initials: R
authors.lastname: Bhat
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: Àngela
authors.initials: À
authors.lastname: Ribes
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Mas
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Aznar
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: Sancenón
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Marcos
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Murguía
authors.affiliation: Materials Chemistry Laboratory, Department of Materials Science and Department of Chemistry, Gulbarga University , Gulbarga-585106, India.
authors.forename: Abbaraju
authors.initials: A
authors.lastname: Venkataraman
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) , Valencia, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Martínez-Máñez
mesh_terms: D000074:Acenocoumarol / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry*; D010421:Pentanoic Acids / Q000737:chemistry; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry*; D014914:Whole Blood Coagulation Time
chemical_list: D000925:Anticoagulants; D010421:Pentanoic Acids; D012822:Silicon Dioxide; D013917:Thrombin; D000074:Acenocoumarol