title: Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma.
abstract: In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and investigate the effects of RAF Abs to IFNg on the specific interaction between Abs to IFNg and IFNg. The experimental conditions were designed and optimal electrode coating, detection circumstances and suitable chaotropic agents for electrode regeneration were selected. The developed technique was found to provide high repeatability, intermediate precision and specificity. The difference between the analytical signals of RAF Ab samples and those of the placebo was up to 50.8%, whereas the difference between non-specific controls and the placebo was within 5%-6%. Thus, the piezoelectric immunosensor as well as ELISA has the potential to be used for detecting the effects of RAF Abs to IFNg on the antigen-antibody interaction, which might be the result of RAF's ability to modify the affinity of IFNg to specific/related Abs.
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. GavrilovaES@materiamedica.ru.
authors.forename: Elena
authors.initials: E
authors.lastname: Don
authors.affiliation: Lipetsk State Technical University, Moskovskaya ul, 30, 398006 Lipetsk, Russian. voronezkaya@gmail.com.
authors.forename: Olga
authors.initials: O
authors.lastname: Farafonova
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. PokhilSE@materiamedica.ru.
authors.forename: Suzanna
authors.initials: S
authors.lastname: Pokhil
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. LubshinaDV@materiamedica.ru.
authors.forename: Darya
authors.initials: D
authors.lastname: Barykina
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. MashkinaMV@materiamedica.ru.
authors.forename: Marina
authors.initials: M
authors.lastname: Nikiforova
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. ShulgaDP@materiamedica.ru.
authors.forename: Darya
authors.initials: D
authors.lastname: Shulga
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. BorshchevaAA@materiamedica.ru.
authors.forename: Alena
authors.initials: A
authors.lastname: Borshcheva
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. TarasovSA@materiamedica.ru.
authors.forename: Sergey
authors.initials: S
authors.lastname: Tarasov
authors.affiliation: Lipetsk State Technical University, Moskovskaya ul, 30, 398006 Lipetsk, Russian. ermolaeva@stu.lipetsk.ru.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Ermolaeva
authors.affiliation: OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. Nauka@materiamedica.ru.
authors.forename: Oleg
authors.initials: O
authors.lastname: Epstein
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007371:Interferon-gamma / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D007371:Interferon-gamma
keywords: interferon-gamma; piezoelectric immunosensors; released-active forms of antibodies