title: Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B.
abstract: A novel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) biosensor was developed to resolve problems associated with conventional LFA strips (e.g., limits in quantitative analysis and low sensitivity). In our SERS-based biosensor, Raman reporter-labeled hollow gold nanospheres (HGNs) were used as SERS detection probes instead of gold nanoparticles. With the proposed SERS-based LFA strip, the presence of a target antigen can be identified through a colour change in the test zone. Furthermore, highly sensitive quantitative evaluation is possible by measuring SERS signals from the test zone. To verify the feasibility of the SERS-based LFA strip platform, an immunoassay of staphylococcal enterotoxin B (SEB) was performed as a model reaction. The limit of detection (LOD) for SEB, as determined with the SERS-based LFA strip, was estimated to be 0.001 ng mL(-1). This value is approximately three orders of magnitude more sensitive than that achieved with the corresponding ELISA-based method. The proposed SERS-based LFA strip sensor shows significant potential for the rapid and sensitive detection of target markers in a simplified manner.
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea. jbchoo@hanyang.ac.kr.
authors.forename: Joonki
authors.initials: J
authors.lastname: Hwang
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea. jbchoo@hanyang.ac.kr.
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea. jbchoo@hanyang.ac.kr.
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D004768:Enterotoxins / Q000032:analysis*; D006046:Gold; D007118:Immunoassay; D057230:Limit of Detection; D054118:Nanospheres; D013059:Spectrum Analysis, Raman
chemical_list: D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D006046:Gold