title: Barcoding markers for Pneumocystis species in wildlife.
abstract: Lung specimens (n = 216) from six wildlife species were examined for occurrence of Pneumocystis species in pulmonary tissues. Among small mammals the shrew Sorex antinorii (80 %) were most frequently colonized. In contrast, foxes and badgers did not yield positive amplification. Host-specificity was noted, at least at the level of the host genus. Phylogenetic trees based on partial mtLSU and mtSSU showed high diversity of species corresponding to animal host diversity. Nuclear rDNA ITS data confirmed unambiguous separation of species. In conclusion, ITS is an excellent marker to distinguish species of the genus Pneumocystis.
authors.affiliation: Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy. Electronic address: pdanesi@izsvenezie.it.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Danesi
authors.affiliation: Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy.
authors.forename: Graziana
authors.initials: G
authors.lastname: da Rold
authors.affiliation: Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige 38010, Italy.
authors.forename: Annapaola
authors.initials: A
authors.lastname: Rizzoli
authors.affiliation: Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige 38010, Italy.
authors.forename: Heidi C
authors.initials: HC
authors.lastname: Hauffe
authors.affiliation: Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Marangon
authors.affiliation: Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; CBS-KNAW Fungal Biodiversity Centre, Utrecht 3584CT, The Netherlands.
authors.forename: Kittipan
authors.initials: K
authors.lastname: Samerpitak
authors.affiliation: Department of Parasitology, Faculty of Biological and Pharmaceutical Sciences, University of Lille, Lille 59006, France; Center for Infection and Immunity of Lille, INSERM, Institut Pasteur de Lille, Lille 59006, France.
authors.forename: Cristine
authors.initials: C
authors.lastname: Demanche
authors.affiliation: Department of Parasitology-Mycology, Dynamyc Research Group, École Nationale Vétérinaire d'Alfort, UPEC, Maisons-Alfort 94700, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Guillot
authors.affiliation: Parasitology-Mycology Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro 35020, Italy.
authors.forename: Gioia
authors.initials: G
authors.lastname: Capelli
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Utrecht 3584CT, The Netherlands; Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam 1090GE, The Netherlands.
authors.forename: Sybren G
authors.initials: SG
authors.lastname: de Hoog
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D058893:DNA Barcoding, Taxonomic; D008168:Lung / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011010:Pneumocystis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology / Q000662:veterinary*
keywords: ITS; Phylogeny; Taxonomy; mtLSU