title: Low p21 level is necessary for the suppressive effects of micoRNA-31 on glioma cell migration and invasion.
abstract: MicroRNAs (miRNAs), a kind of endogenous non-coding RNAs, regulate gene expression through binding to the 3'-untranslational region (UTR) of target messenger RNAs (mRNAs) and act as endogenous agents of RNA interference, resulting in either mRNA degradation or translational repression. MiR-31 has been demonstrated to be associated with the development and progression of glioma. However, the underlying molecular mechanism remains largely unclear. In the present study, we demonstrated that miR-31 only inhibited the cell migration and invasion, as well as the expression of a known miR-31 target oncogene radixin, in U251 glioma cells that expressed low level of p21; however, miR-31 showed no above effects on glioma SHG44 cells that highly expressed p21. Moreover, upregulation of p21 in U251 cells reversed the suppressive effects of miR-31 on the cell migration and invasion, suggesting that low p21 level is necessary for the miR-31-mediated inhibitory effects on glioma. Furthermore, analysis for 35 glioma specimens showed that the expression of radixin was negatively correlated with the miR-31 level in glioma tissues with low p21 expression; however, no such correlation was found in glioma tissues with high p21 level, further supporting that the low p21 level is necessary for the suppressive effect of miR-31 on the expression of its target oncogenes. In summary, our study demonstrates that the suppressive effect of miR-31 on glioma cell migration and invasion is p21-dependent, and suggests that miR-31 may be used for the treatment of patients with p21-deficent glioma.
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong, 510282, China.|Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangdong, 510282, China.|Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, Guangzhou, 510282, Guangdong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong, 510282, China.|Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangdong, 510282, China.|Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, Guangzhou, 510282, Guangdong, China.
authors.forename: Fengfei
authors.initials: F
authors.lastname: Lu
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong, 510282, China.|Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangdong, 510282, China.|Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, Guangzhou, 510282, Guangdong, China.
authors.forename: Hongchao
authors.initials: H
authors.lastname: Xu
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong, 510282, China.|Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangdong, 510282, China.|Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, Guangzhou, 510282, Guangdong, China.
authors.forename: Qifu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong, 510282, China.|Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangdong, 510282, China.|Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, Guangzhou, 510282, Guangdong, China.
authors.forename: Chunnan
authors.initials: C
authors.lastname: Lin
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong, 510282, China. zhangszdr@yeah.net.|Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangdong, 510282, China. zhangszdr@yeah.net.|Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, Guangzhou, 510282, Guangdong, China. zhangszdr@yeah.net.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation / Q000235:genetics
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; C071776:radixin
keywords: Glioma; Invasion; MicroRNA-31; Migration; p21