title: Characterization and analysis of differentially expressed microRNAs in hircine ovaries during the follicular and luteal phases.
abstract: Ovarian activity, which is mainly controlled by follicle-stimulating hormone and luteinizing hormone, is vital to successful reproduction and maintaining reproductive efficiency in livestock. To determine if the regulation of follicular-luteal transition occurs at the post-transcriptional level in hircine ovaries, the expression patterns of small RNAs in the ovarian tissues of Anhui white goats in the follicular and luteal phases were analyzed using Solexa sequencing. In total, 1039 miRNAs were co-expressed in the two libraries, and 278 and 469 miRNAs were specifically expressed in the hircine ovaries during the follicular and luteal phases, respectively. A total of 43 potential novel miRNAs were predicted in the two libraries. GO annotation and KEGG pathway analysis were applied to analyze the target genes of all miRNAs predicted in the two libraries. The highly and differentially expressed miRNAs included miR-26-5p, miR-145-5p, miR-145, miR-145a-5p, miR-125a-5p, miR-320d, and miR-320c, which may participate in follicular-luteal transition. Five co-expressed miRNAs, of which 2 were differentially expressed between the two libraries, were randomly selected to validate the expression pattern using RT-PCR, and the results were consistent with the Solexa sequencing data. Our present results help to clarify the roles of miRNAs in the regulation of follicular-luteal transition in goat ovaries, which may further enhance the reproductive efficiency of commercially important animals in the future.
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, 230036 Hefei, PR China; Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang West Road, 230036 Hefei, PR China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Ling
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, 230036 Hefei, PR China; Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, PR China.
authors.forename: X F
authors.initials: XF
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, 230036 Hefei, PR China; Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang West Road, 230036 Hefei, PR China.
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, 230036 Hefei, PR China; Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang West Road, 230036 Hefei, PR China.
authors.forename: J P
authors.initials: JP
authors.lastname: Ding
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, PR China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Ma
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, PR China.
authors.forename: M X
authors.initials: MX
authors.lastname: Chu
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, PR China.
authors.forename: R
authors.initials: R
authors.lastname: Di
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, 230036 Hefei, PR China; Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang West Road, 230036 Hefei, PR China. Electronic address: zhangyunhai01@126.com.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, 230036 Hefei, PR China; Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang West Road, 230036 Hefei, PR China. Electronic address: zhangxiaorong01@163.com.
authors.forename: X R
authors.initials: XR
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005260:Female; D005498:Follicular Phase / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D008183:Luteal Phase / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*
chemical_list: D035683:MicroRNAs
keywords: Capra hircus; Differentially expressed miRNAs; Follicular phase; Luteal phase; Ovary