title: Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera.
abstract: Sacbrood virus(SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera In previous studies, SBV isolates infecting A. cerana(AcSBV) and SBV isolates infecting A. mellifera(AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318A. mellifera colonies and 64A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38A. mellifera colonies and 37A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated fromA. mellifera were clustered with theA. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae ofA. mellifera, although there were no obvious signs of the disease, demonstrating the replication of AcSBV inA. mellifera Our results suggest that AcSBV is able to infectA. melliferacolonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities ofA. cerana and A. melliferato sacbrood disease and is potentially useful for guiding beekeeping practices.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Hong-Ri
authors.initials: HR
authors.lastname: Gong
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xiu-Xian
authors.initials: XX
authors.lastname: Chen
authors.affiliation: USDA-ARS, Bee Research Laboratory, Beltsville, Maryland, USA.
authors.forename: Yan Ping
authors.initials: YP
authors.lastname: Chen
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Fu-Liang
authors.initials: FL
authors.lastname: Hu
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Jiang-Lin
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Zhe-Guang
authors.initials: ZG
authors.lastname: Lin
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Yu
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China hqzheng@zju.edu.cn.
authors.forename: Huo-Qing
authors.initials: HQ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D016000:Cluster Analysis; D005838:Genotype; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012324:RNA-Dependent RNA Polymerase