title: A transcriptional miRNA-gene network associated with lung adenocarcinoma metastasis based on the TCGA database.
abstract: Lung adenocarcinoma is the most common subtype of non-small cell lung cancer (NSCLC), leading to the largest number of cancer-related deaths worldwide. The high mortality rate may be attributed to the delay of detection. Therefore, it is of great importance to explore the mechanism of lung adenocarcinoma metastasis and the strategy to block metastasis of the disease. We searched and downloaded mRNA and miRNA expression data and clinical data from The Cancer Genome Atlas (TCGA) database to identify differences in mRNA and miRNA expression of primary tumor tissues from lung adenocarcinoma that did and did not metastasize. In addition, combined with bioinformatic prediction, we constructed an miRNA-target gene regulatory network. Finally, we employed RT-qPCR to validate the bioinformatic approach by determining the expression of 10 significantly differentially expressed genes which were also putative targets of several dysregulated miRNAs. RT-qPCR results indicated that the bioinformatic approach in our study was acceptable. Our data suggested that some of the genes including PKM2, STRAP and FLT3, may participate in the pathology of lung adenocarcinoma metastasis and could be applied as potential markers or therapeutic targets for lung adenocarcinoma.
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Han
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Kunlin
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Hengyi
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: He
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D014408:Biomarkers, Tumor / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger