title: Immune Response to Double Dose Hepatitis-B Vaccine using Four Dose Schedule in HIV Infected Children.
abstract: OBJECTIVE
  To assess the immune response to hepatitis B vaccination in HIV infected children using 4 double dose schedule administered at 0-1-2-6 mo.
  
  
  METHODS
  In a prospective observational study, 40 HIV infected children were vaccinated with hepatitis B virus (HBV) vaccine (20 mcg, 1 ml IM at anterolateral aspect of thigh of recombinant DNA vaccine) at 0-1-2-6 mo. Anti-HBsAb titre were assessed 4-8 wk after the last dose. Clinical severity was assessed according to WHO staging. Immune status of the patients was assessed using CD 4+ counts before the vaccination.
  
  
  RESULTS
  Of the total 40 patients, 33 (28 boys, 5 girls) completed the study. Six patients were in pre-antiretroviral therapy (ART) care while 27 were receiving ART for a mean duration of 2.06 y. Ten patients belonged to WHO clinical stage 1, and 17, 2 and 4 patients to WHO clinical stage 2, 3 and 4 respectively. Median CD4+ cell count was 738/mm(3) and 28 patients had mild or no immunosuppression. Out of the total 33 patients who completed followup, only 2 patients (6 %) did not seroconvert (Anti HBsAb titre at end of study <10 IU/L), the rest (94 %) achieved different levels of Anti HBsAb titre at end of study. Twenty two (66 %) patients had anti HBsAb titre more than 1000 IU/L, 8 (24 %) had titre between 100 and 1000 IU/L and one (3 %) patient had level of 10-99.99 IU/L.
  
  
  CONCLUSIONS
  In HIV-infected children who have no or mild immunosuppression, four dose, double dose schedule of HBV vaccine achieves very high seroconversion rates.
authors.affiliation: Department of Pediatrics, Lady Hardinge Medical College & Associated Kalawati Saran Children's Hospital, New Delhi, 110001, India.
authors.forename: Debarshi
authors.initials: D
authors.lastname: Bose
authors.affiliation: Department of Pediatrics, Lady Hardinge Medical College & Associated Kalawati Saran Children's Hospital, New Delhi, 110001, India. jchandra55@gmail.com.
authors.forename: Jagdish
authors.initials: J
authors.lastname: Chandra
authors.affiliation: Department of Microbiology, Lady Hardinge Medical College , New Delhi, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Dutta
authors.affiliation: Department of Microbiology, Lady Hardinge Medical College , New Delhi, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Jais
authors.affiliation: Department of Pediatrics, Lady Hardinge Medical College & Associated Kalawati Saran Children's Hospital, New Delhi, 110001, India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Ray
authors.affiliation: Department of Pediatrics, Lady Hardinge Medical College & Associated Kalawati Saran Children's Hospital, New Delhi, 110001, India.
authors.forename: Rohini Ajay
authors.initials: RA
authors.lastname: Gupta
authors.affiliation: Department of Pediatrics, Lady Hardinge Medical College & Associated Kalawati Saran Children's Hospital, New Delhi, 110001, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Seth
authors.affiliation: Department of Pediatrics, Lady Hardinge Medical College & Associated Kalawati Saran Children's Hospital, New Delhi, 110001, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Kumar
mesh_terms: D005260:Female; D015658:HIV Infections / Q000276:immunology*; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011446:Prospective Studies
chemical_list: D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines
keywords: HIV-infected children; Hepatitis B vaccine; Seroconversion