title: Morphological study of rat skin flaps treated with subcutaneous dimethyl sulfoxide combined with hyperbaric oxygen therapy.
abstract: This study investigated the effects of hyperbaric oxygen therapy (HBOT) and dimethyl sulfoxide (DMSO) in tissue necrosis, genotoxicity, and cell apoptosis. Random skin flaps were made in 50 male Wistar rats, randomly divided into the following groups. Control group (CT), wherein a rectangular skin section (2 x 8 cm) was dissected from the dorsal muscle layer, preserving the cranial vessels, lifted, and refixed to the bed; distilled water (DW) group, in which DW was injected into the distal half of the skin flap; DMSO group, wherein 5% DMSO was injected; HBOT group, comprising animals treated only with HBOT; and HBOT + DMSO group, comprising animals treated with 100% oxygen at 2.5 atmospheres absolute for 1 h, 2 h after the experiment, daily for 10 consecutive days. A skinflap specimen investigated by microscopy. The percentage of necrosis was not significantly different between groups. The cell viability index was significantly different between groups (P < 0.001): 87.40% (CT), 86.20% (DW), 84.60% (DMSO), 86.60% (DMSO + HBO), and 91% (HBO) (P < 0.001), as was the cell apoptosis index of 12.60 (CT), 12.00 (DW), 15.40 (DMSO), 9.00 (HBO), and 12.00 (DMSO + HBO) (P < 0.001). The genotoxicity test revealed the percentage of cells with DNA damage to be 22.80 (CT), 22.60 (DW), 26.00 (DMSO), 8.80 (DMSO + HBO), and 7.20 (HBO) (P < 0.001). Although the necrotic area was not different between groups, there was a significant reduction in the cellular DNA damage and apoptosis index in the HBOT group.
authors.affiliation: Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: K G
authors.initials: KG
authors.lastname: Almeida
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo do Hospital Universitário, Universidade Aberta do Brasil.
authors.forename: R J
authors.initials: RJ
authors.lastname: Oliveira
authors.affiliation: Universidade Anhanguera, Campo Grande, MS, Brasil.
authors.forename: D M
authors.initials: DM
authors.lastname: Dourado
authors.affiliation: Serviço de Cirurgia Plástica da Santa Casa de Misericórdia de Campo Grande, Campo Grande, MS, Brasil.
authors.forename: E A
authors.initials: EA
authors.lastname: Filho
authors.affiliation: Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Laboratório de Parasitologia Humana, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: W S
authors.initials: WS
authors.lastname: Fernandes
authors.affiliation: Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: A S
authors.initials: AS
authors.lastname: Souza
authors.affiliation: Programa de Pós-graduação em Farmácia, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: F H S
authors.initials: FH
authors.lastname: Araújo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage*; D006801:Humans; D006931:Hyperbaric Oxygenation; D008297:Male; D009336:Necrosis / Q000473:pathology / Q000628:therapy*; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013524:Surgical Flaps / Q000473:pathology*
chemical_list: D004121:Dimethyl Sulfoxide