title: Antioxidant Potential of Plumieride against CCl₄-Induced Peroxidative Damage in Rats.
abstract: In search of a new potent as an antioxidant from natural sources, plumieride-an iridoid isolated from the methanol extract of the bark of Plumeria bicolor (family Apocynaceae) was evaluated for its antioxidant potential against CCl₄-induced peroxidative damage in liver of rats. The antioxidant potential was evaluated by using hepatic tissue for SOD (superoxide dismutase), CAT (catalase), GSH (reduced glutathione), GPx (glutathione peroxidase), GR (glutathione reductase) and LPO (lipid peroxidation) alongwith the concomitant blood serum for AST &amp; ALT (aspartate and alanine transaminases), GGT (gamma glutamyl transpeptidase), ALP (alkaline phosphatase), total bilirubin and total protein contents. All the biochemical parameters were significantly (p ≤ 0.001) altered by CCl₄ (0.3 mL/kg body weight/twice a week, intra-peritoneally for 30 days). Simultaneously, oral treatment with plumieride (5, 10 and 20 mg/kg body weight/day for 30 days), restored all the parameters towards a normal level, remarkably. The histological findings of liver sections further corroborated the antioxidant potential of plumieride compared with standard drug-silymarin. In conclusion, plumieride consists of sugar molecules, which have alcoholic groups. Therefore, the alcoholic groups of sugar increase its antioxidant potential through intermolecular hydrogen bonding along with the thiol(SH) group of non-protein thiols and enzymes resulting in the restoration of the antioxidant system. Therefore, it might be considered a natural antioxidant against peroxidative damage in rats.
authors.affiliation: Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur 302 055, India. d.singh2009@gmail.com.|Department of Zoology, Dyal Singh College, University of Delhi, Lodhi Road, New Delhi 110 003, India. d.singh2009@gmail.com.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Zoology, Dyal Singh College, University of Delhi, Lodhi Road, New Delhi 110 003, India. zoology.dsc@gmail.com.
authors.forename: Priya Vrat
authors.initials: PV
authors.lastname: Arya
authors.affiliation: Department of Chemistry, University of Rajasthan, Jaipur 302 055, India. ashutoshs_912@rediffmail.com.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Zoology, Dyal Singh College, University of Delhi, Lodhi Road, New Delhi 110 003, India. vpa541@gmail.com.
authors.forename: Ved Prakash
authors.initials: VP
authors.lastname: Aggarwal
authors.affiliation: Department of Chemistry, University of Rajasthan, Jaipur 302 055, India. mpdobhal@yahoo.com.
authors.forename: Mahabeer Prasad
authors.initials: MP
authors.lastname: Dobhal
authors.affiliation: Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur 302 055, India. gupta_rs@hotmail.com.
authors.forename: Radhey Shyam
authors.initials: RS
authors.lastname: Gupta
keywords: antioxidants; carbon tetrachloride; free radicals; marker enzymes; plumieride