title: Porcine lactoferrin-derived peptide LFP-20 protects intestinal barrier by maintaining tight junction complex and modulating inflammatory response.
abstract: LFP-20, a 20-amino acid antimicrobial peptide in the N terminus of porcine lactoferrin, has antimicrobial and immunomodulatory activities. This study assessed the protective effects of LFP-20 on LPS-induced intestinal damage in a LPS-induced mouse model and in vitro, using intestinal porcine epithelial cell line 1 (IPEC-1) cells. LFP-20 prevented LPS-induced impairment in colon epithelium tissues, infiltration of macrophages or leukocytes, histological evidence of inflammation and increased levels of TNF-a, IL-6 and IFN-γ. LFP-20 increased the expression of zonula occludens-1, occludin and claudin-1 and reduced permeability as well as apoptosis of the colon in LPS-treated mice. In IPEC-1 cells, LFP-20 increased transepithelial electrical resistance and tight junction expression. Moreover, we found LFP-20 decreased the MyD88 and AKT levels to affect the NF-κB signaling pathway, to modulate inflammation response and tight junction networks in the processing of LPS stimulation. In summary, LFP-20 prevents the inflammatory response and disruption of tight junction structure induced by LPS, suggesting the potential use of LFP-20 as a prophylactic agent to protect intestinal barrier function.
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zong
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wangyang
authors.initials: W
authors.lastname: Hu
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Deguang
authors.initials: D
authors.lastname: Song
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Du
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: yzwang321@zju.edu.cn.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D003092:Colitis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D016207:Cytokines / Q000276:immunology; D004731:Endotoxins / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D007113:Immunity, Innate / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D007781:Lactoferrin / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D013552:Swine; D019108:Tight Junctions / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D057705:Transendothelial and Transepithelial Migration / Q000187:drug effects
chemical_list: D016207:Cytokines; D004731:Endotoxins; C000606576:LFP-20 peptide, porcine; C044101:endotoxin, Escherichia coli; D007781:Lactoferrin
keywords: Inflammation; Intestinal barrier; LFP-20; NF-κB; Tight junction