title: Immunohistochemical Profile of Uterine Leiomyoma With Bizarre Nuclei; Comparison With Conventional Leiomyoma, Smooth Muscle Tumors of Uncertain Malignant Potential and Leiomyosarcoma.
abstract: OBJECTIVE
  To assess the immunohistochemical profile of the atypical nuclei in leiomyoma with bizarre nuclei and compare with benign and malignant counterparts.
  
  
  METHODS
  26 cases of uterine smooth muscle tumors including 12 leiomyosarcoma(LMS), 10 leiomyoma with bizarre nuclei (LBN) and 4 smooth muscle tumor with uncertain malignant potential (STUMP) were selected using whole tissue sections for this study and analysis. Six cases of ordinary leiomyoma were included as benign control group. All representative section were stained for P53, Ki67, estrogen receptor and progestrone receptor. Analysis was carried out using SPSS 16.0 for windows software.
  
  
  RESULTS
  Six out of 12 cases of LMS showed strong and diffuse nuclear staining with p53 antibody (50%). In contrast none of STUMPs and only one case of LBN cases showed focal positive reaction with P53. Percentage of positive cells for ki67 in LMS was 14.92 while only 0.85% of cells in LBNs was labeled with Ki67 proliferative marker. (P<0.001). Regarding steroid hormone receptors a significant loosing trend was found in these receptors from benign toward malignant tumors through LBN and STUMP cases.
  
  
  CONCLUSIONS
  Loss of inhibitory function of wild type P53 gene in leiomyosarcoma is an essential event that discriminate frankly malignant tumors from STUMP and atypical leiomyoma.
authors.affiliation: Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Dastranj Tabrizi
authors.affiliation: Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Ghojazadeh
authors.affiliation: Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hanieh
authors.initials: H
authors.lastname: Thagizadeh Anvar
authors.affiliation: Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Vahedi
authors.affiliation: Pathology Department, Urmieh University of Medical Sciences, Urmieh, Iran.
authors.forename: Siamak
authors.initials: S
authors.lastname: Naji
authors.affiliation: Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Elmira
authors.initials: E
authors.lastname: Mostafidi
authors.affiliation: Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Siamak
authors.initials: S
authors.lastname: Berenjian
keywords: Leiomyoma; Leiomyosarcoma; P53 gene; Smooth muscle tumors