title: Effects of stem cell therapy on protein profile of parkinsonian rats using an(18) O-labeling quantitative proteomic approach.
abstract: The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with (18) O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Kefu
authors.initials: K
authors.lastname: Liu
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Qing
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D058953:Neural Stem Cells / Q000637:transplantation*; D010103:Oxygen Isotopes / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism* / Q000628:therapy*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010103:Oxygen Isotopes; D020543:Proteome
keywords: 18O-labeling; Animal proteomics; Neural stem cell; Parkinson's disease; Quantitative proteomics; Therapy