title: Effects of phosphate supplementation on Pseudomonas aeruginosa invasive behavior in burn wound infections: A simple approach to a big problem.
abstract: This study was designed to investigate the effect of inorganic phosphate supplementation on invasive behavior of Pseudomonas aeruginosa in burn wound infections. An emulsion-based lotion containing sodium dihydrogen phosphate was formulated and then 50 female Sprague-Dawley rats with burn wounds were used to assess the effect of phosphate supplementation on swarming motility of P. aeruginosa. On the second day after burn, four groups of rats were inoculated with P. aeruginosa and one group was left as negative control. The treatment was started on day 3 and the animals were followed up for 4 weeks. Significant improvement in wound healing was observed in the phosphate-receiving group after the 4-week follow-up, compared to the negative control, positive control, and silver sulfadiazine-receiving groups. Histopathological assessment of the tissue samples also indicated the healing process in phosphate-enriched lotion receiving group. The results showed that inorganic phosphate supplementation results in alteration of the virulence behavior of P. aeruginosa and improvement in the wound healing process. In conclusion, phosphate supplementation would be a rational strategy in the eradication of P. aeruginosa wound infection.
authors.affiliation: Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Science, Shiraz, Iran. Electronic address: smsamani@sums.ac.ir.
authors.forename: Soliman
authors.initials: S
authors.lastname: Mohammadi-Samani
authors.affiliation: Faculty of Paramedical Sciences, Shiraz University of Medical Science, Shiraz, Iran.
authors.forename: Shahriyar
authors.initials: S
authors.lastname: Kouroshfard
authors.affiliation: Shiraz Transplant Research Center, Shiraz University of Medical Science, Shiraz, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Azarpira
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002056:Burns; D005260:Female; D010710:Phosphates / Q000494:pharmacology*; D011552:Pseudomonas Infections; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012837:Silver Sulfadiazine / Q000494:pharmacology; D063465:Skin Cream; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection
chemical_list: D000891:Anti-Infective Agents, Local; D010710:Phosphates; C018279:sodium phosphate; D012837:Silver Sulfadiazine
keywords: Burn; Microbial resistance; Phosphate; Pseudomonas