title: Leptin expression in mandarin fish Siniperca chuatsi (Basilewsky): Regulation by postprandial and short-term fasting treatment.
abstract: Most fish species possess duplicate leptin genes (LEP). Mandarin fish (Siniperca chuatsi) leptin A gene (sLEP-A) have been cloned in the previous study. In the present study, we cloned and characterized leptin B gene (sLEP-B) in mandarin fish, including a 471bp open reading frame (ORF) encoding a 158-amino acid protein. The three-dimensional (3D) structural model of sLEP-B protein showed a highly conserved of tertiary structure similar to that of other vertebrates. Genomic sequencing results indicated that sLEP-B possessed only one intron. This is the first report of the loss of an intron in LEP-B in Perciformes. The different distribution patterns of sLEPs suggest different physiological roles of these two genes. The presence of HNF3β, a liver-enriched transcription factor, only in sLEP-A indicated abundant expression and metabolic function of sLEP-A in the liver. In an in vivo experiment, the expressions of brain sLEP-A and sLEP-B were observed to increase after a meal. During the short-term fasting, the expressions of sLEPs in mandarin fish brain were decreased significantly. A persistent and significant increase in hepatic sLEP-A expression supported a relationship between leptin and food intake in mandarin fish. These results suggest that sLEP-A plays an important role in the regulation of energy homeostasis in this carnivorous fish, and sLEP-B is probably a specialized gene responsible for the central nervous system (CNS) control of energy regulation.
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Yuan
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China.
authors.forename: Aixuan
authors.initials: A
authors.lastname: Li
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China. Electronic address: xufang_liang@hotmail.com.
authors.forename: Xu-Fang
authors.initials: XF
authors.lastname: Liang
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China.
authors.forename: Shan
authors.initials: S
authors.lastname: He
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei 430070, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519, USA.
authors.forename: Ya-xiong
authors.initials: YX
authors.lastname: Tao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D005215:Fasting; D005399:Fishes / Q000235:genetics*; D020738:Leptin / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D019518:Postprandial Period; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D020738:Leptin
keywords: Energy regulation; Gene structure; Intron loss; Postprandial; Tissue expression