title: MicroRNA-509-3p increases the sensitivity of epithelial ovarian cancer cells to cisplatin-induced apoptosis.
abstract: OBJECTIVE
  XIAP is upregulated in chemoresistant epithelial ovarian cancer (EOC). However, the molecular mechanism of this dysregulation remains unclear.
  
  
  METHODS
  The regulation of XIAP by miR-509-3p was investigated by luciferase reporter assay, real-time PCR and immunobloting, and the roles of miR-509-3p in cellular proliferation and apoptosis were accessed through MTT and DAPI assays.
  
  
  RESULTS
  miR-509-3p, a downregulated miRNA in chemoresistant EOC, can directly target the XIAP via its 3'UTR. Overexpression of miR-509-3p can not only downregulate the expression of XIAP in ovarian cancer cells but also inhibit the proliferation of EOC cells and increase their sensitivity to cisplatin-induced apoptosis.
  
  
  CONCLUSIONS
  Our data suggest that restoring certain dysregulated miRNAs to their normal levels could increase the therapeutic effects of anticancer drugs.
authors.affiliation: Department of Gynecology, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Wenshu
authors.initials: W
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Li
authors.affiliation: Gene Science & Health Company, Shenzhen, China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Mengdie
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Gynecology, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Longshu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Songshan
authors.initials: S
authors.lastname: Jiang
mesh_terms: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D049410:Luciferases, Renilla / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D010051:Ovarian Neoplasms; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents; C571982:MIRN509 microRNA, human; D035683:MicroRNAs; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D049410:Luciferases, Renilla; D002945:Cisplatin
keywords: X-linked inhibitor of apoptosis; cell apoptosis; cell proliferation; microRNA-509–3p; ovarian cancer