title: A subset of enteroendocrine cells is activated by amino acids in the Drosophila midgut.
abstract: The intestine is involved in digestion and absorption, as well as the regulation of metabolism upon sensation of the internal intestinal environment. Enteroendocrine cells are thought to mediate these internal intestinal chemosensory functions. Using the CaLexA (calcium-dependent nuclear import of LexA) method, we examined the enteroendocrine cell populations that are activated when flies are subjected to various dietary conditions such as starvation, sugar, high fat, protein, or pathogen exposure. We find that a specific subpopulation of enteroendocrine cells in the posterior midgut which express Dh31 and tachykinin are activated by the presence of proteins and amino acids.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea.
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea.
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea.
authors.forename: Sooin
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University, Suwon, Korea.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Ahn
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University, Suwon, Korea.
authors.forename: KyeongJin
authors.initials: K
authors.lastname: Kang
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea.
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kwon
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004040:Dietary Carbohydrates / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000378:metabolism*; D005260:Female; D007301:Insect Hormones / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D015320:Tachykinins / Q000378:metabolism
chemical_list: D000596:Amino Acids; C434606:Dh31 protein, Drosophila; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins; D029721:Drosophila Proteins; D007301:Insect Hormones; D015320:Tachykinins
keywords: CaLexA system; Drosophila melanogaster; amino acids; chemosensing; enteroendocrine cells