title: miR-9 Mediates CALHM1-Activated ATP-P2X7R Signal in Painful Diabetic Neuropathy Rats.
abstract: In this study, we planned to illuminate the mechanisms of the expression and function of CALHM1 in painful diabetic neuropathy (PDN). PDN rat model was constructed. The expression of CALHM1 and miR-9 in rat spinal dorsal horn neurons was detected. The correlation between the level of CALHM1 mRNA and 50 % PWT and the relationship between the expression of CALHM1 and miR-9 in rat spinal dorsal horn neurons were statistically analyzed. The effect of miR-9 and CALHM1 on each other's expression in PDN rat spinal dorsal horn neurons were tested by qRT-PCR or Western blot. The co-culture system of neurons and glias from PDN rat spinal dorsal horn was constructed. The concentration of calcium and ATP as well as the expression of P2X7 receptor regulated by CALHM1 and miR-9 in PDN rat spinal dorsal horn neurons was measured. The results showed that the expression of CALHM1 was increased in PDN rat compared with controls, while its mRNA level was negatively correlated with 50 % PWT. miR-9, which was also upregulated in the spinal dorsal horn neurons of PDN rats, was positively correlated with the expression of CALHM1. The concentration of calcium and ATP as well as the expression of P2X7 receptor in glias was also increased in PDN rats. These increases could be reverted by inhibiting CALHM1 and/or miR-9. CALHM1 is involved in miR-9-mediated ATP-P2X7 pathway between neurons and glias in PDN rat.
authors.affiliation: Anesthesia Department, The First Affiliated Hospital of Nanhua University, Chuanshan Road 69, Hengyang, 421001, Hunan, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Liu
authors.affiliation: Health School, The Affiliated Nanhua Hospital of Nanhua University, Dongfeng South Road 336, Hengyang, 421001, Hunan, China.
authors.forename: Qinying
authors.initials: Q
authors.lastname: Ao
authors.affiliation: Anesthesia Department, Xiangya Hospital of Central South University, Xiangya Road 87, Changsha, 410008, Hunan, China.
authors.forename: Qulian
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Anesthesia Department, The First People's Hospital of Foshan City, Lingnan Avenue North 81, Foshan, 528000, Guangdong, China.
authors.forename: Wanyou
authors.initials: W
authors.lastname: He
authors.affiliation: Anesthesia Department, The First Affiliated Hospital of Nanhua University, Chuanshan Road 69, Hengyang, 421001, Hunan, China.
authors.forename: Liangyu
authors.initials: L
authors.lastname: Peng
authors.affiliation: Anesthesia Department, The First Affiliated Hospital of Nanhua University, Chuanshan Road 69, Hengyang, 421001, Hunan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Anesthesia Department, The First Affiliated Hospital of Nanhua University, Chuanshan Road 69, Hengyang, 421001, Hunan, China. xiaolinghu0734@hotmail.com.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology; D003929:Diabetic Neuropathies / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D001786:Blood Glucose; C000620794:CALHM1 protein, rat; D015220:Calcium Channels; C582333:MIRN9 microRNA, rat; D035683:MicroRNAs; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate
keywords: CALHM1; Diabetes mellitus; P2X7 receptor; Painful neuropathy; miR-9