title: Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration.
abstract: NMR characterization of many proteins is limited by low expression, hurdles for deuteration, and poor sample homogeneity. We introduce a set of high-dimensionality proton-detected experiments developed for unambiguous resonance assignments of such proteins, which we could successfully apply to a 1 mg amount of non-deuterated Tau paired helical filaments.
authors.affiliation: Max-Planck Institute for Biophysical Chemistry, Department NMR-Based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany. rali@nmr.mpibpc.mpg.de.
authors.forename: ShengQi
authors.initials: S
authors.lastname: Xiang
authors.affiliation: DZNE, German Center for Neurodegenerative Diseases, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany and CAESAR Research Center, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.
authors.forename: Jacek
authors.initials: J
authors.lastname: Biernat
authors.affiliation: DZNE, German Center for Neurodegenerative Diseases, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany and CAESAR Research Center, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.
authors.forename: Eckhard
authors.initials: E
authors.lastname: Mandelkow
authors.affiliation: Max-Planck Institute for Biophysical Chemistry, Department NMR-Based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany. rali@nmr.mpibpc.mpg.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
authors.affiliation: Max-Planck Institute for Biophysical Chemistry, Department NMR-Based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany. rali@nmr.mpibpc.mpg.de.
authors.forename: Rasmus
authors.initials: R
authors.lastname: Linser
mesh_terms: D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D016875:tau Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D011506:Proteins; D016875:tau Proteins