title: A Novel Controlled Release Immunosensor based on Benzimidazole Functionalized SiO2 and Cyclodextrin Functionalized Gold.
abstract: A novel controlled release system-based sandwich-type immunosensor is fabricated to detect squamous cell carcinoma antigen (SCCA). The 1-methyl-1H-benzimidazole functionalized mesoporous SiO2 (MBI-MS) is used to load methylene blue (MB). β-cyclodextrin functionalized gold (CD-Au) is introduced as the gatekeeper for encapsulating MB and capturing the adamantly functional detection antibody (ADA-Ab2). And pH stimulus serves as the trigger system to control the MB release. After the load of MB, the CD-Au blocks the pores of the MBI-MS by the host-guest interaction in the neutral condition. However, when the pH is below 7.0, CD-Au is separated from the surface of MBI-MS owing to the protonation of the aromatic amines. The encapsulated MB is released from the pores of MBI-MS and detected by square wave voltammetry. The controlled release immunosensor shows a relatively wide linear range from 0.001 to 20 ng · mL(-1) with a low detection limit of 0.25 pg · mL(-1). The immunosensor also shows good reproducibility and selectivity, which endows it broad application prospect in clinical research.
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D001562:Benzimidazoles / Q000737:chemistry*; D015374:Biosensing Techniques; D003692:Delayed-Action Preparations; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D016062:Porosity; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D001562:Benzimidazoles; D003692:Delayed-Action Preparations; D047392:beta-Cyclodextrins; D006046:Gold; D012822:Silicon Dioxide; C031000:benzimidazole