title: Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles.
abstract: MicroRNAs (miRNAs) play important roles in the osteogenic differentiation of stem cells. However, the application of miRNA in bone regeneration has been limited by its poor stability, low cellular uptake, and undesired immune response. In this study, chitosan (CS)/tripolyphosphate (TPP)/Hyaluronic Acid (HA) nanoparticles (CTH NPs) were prepared to deliver antimiR-138 to bone marrow mesenchymal stem cells (MSCs). The particle size, polydispersity index, and zeta potential of CTH NPs were related to the weight ratio of CS:TPP:HA. At optimum N/P ratio (20:1), the highest encapsulation efficiency was obtained. Both blank CTH NPs and CTH/antmiR-138 NPs exhibited no cytotoxicity to MSCs. A high transfection efficiency (nearly 70%) and significant enhancement of the osteogenesis of MSCs were observed. Above results demonstrated that CTH NPs was a potential candidate as an efficient non-viral miRNA vector to regulate the osteogenic differentiation of MSCs.
authors.affiliation: State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China; College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China; Qingdao First Sanatorium, Jinan Military Region, No. 27 West Hong Kong Road, Qingdao 266071, Shandong Province, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Wu
authors.affiliation: College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.affiliation: Qingdao First Sanatorium, Jinan Military Region, No. 27 West Hong Kong Road, Qingdao 266071, Shandong Province, China.
authors.forename: Guangyan
authors.initials: G
authors.lastname: Hui
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors.forename: Lankun
authors.initials: L
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. Electronic address: yznmbk@fmmu.edu.cn.
authors.forename: Qintao
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China. Electronic address: xgchen@ouc.edu.cn.
authors.forename: Xiguang
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006820:Hyaluronic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D010316:Particle Size; D011122:Polyphosphates / Q000737:chemistry; D051381:Rats; D014162:Transfection
chemical_list: D015703:Antigens, CD; D004337:Drug Carriers; D035683:MicroRNAs; D011122:Polyphosphates; D006820:Hyaluronic Acid; D048271:Chitosan; C005692:triphosphoric acid
keywords: Chitosan; Chitosan (PubChem CID: 21896651); Hyaluronic acid (PubChem CID: 24728612); Mesenchymal stem cells; Nanoparticle; Osteogenic differentiation; microRNA