title: Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery.
abstract: Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after BLAST. Of these, 24,010 (64.74%) were annotated in the National Center for Biotechnology Information database, and 3715 simple sequence repeats and 2008 single nucleotide polymorphisms were identified in this EST dataset as potential molecular markers. This study provides new data for functional genomic and biological research on grass carp. The markers identified in this study will enrich the currently used molecular markers and facilitate marker-assisted selection in grass carp-breeding programs. These results also demonstrate that transcriptomic analysis based on 454 sequencing is a powerful tool for gene discovery and molecular marker development in non-model species.
authors.affiliation: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture;
authors.forename: L Y
authors.initials: LY
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture;
authors.forename: J J
authors.initials: JJ
authors.lastname: Bai
authors.affiliation: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture;
authors.forename: J J
authors.initials: JJ
authors.lastname: Fan
authors.affiliation: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture;
authors.forename: D M
authors.initials: DM
authors.lastname: Ma
authors.affiliation: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture;
authors.forename: Y C
authors.initials: YC
authors.lastname: Quan
authors.affiliation: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture;
authors.forename: P
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002347:Carps / Q000235:genetics*; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D005819:Genetic Markers; D006066:Gonads / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D009132:Muscles / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers