title: A Rapid Screening Analysis of Antioxidant Compounds in Native Australian Food Plants Using Multiplexed Detection with Active Flow Technology Columns.
abstract: Conventional techniques for identifying antioxidant and phenolic compounds in native Australian food plants are laborious and time-consuming. Here, we present a multiplexed detection technique that reduces analysis time without compromising separation performance. This technique is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. Extracts from cinnamon myrtle (Backhousia myrtifolia) and lemon myrtle (Backhousia citriodora) leaves were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, mass spectroscopy (MS), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH(•)) derivatisation for antioxidants as detection methods. A number of antioxidant compounds were detected in the extracts of each leaf extract.
authors.affiliation: Food Science and Technology Research Group, School of Science and Health, Western Sydney University (Hawkesbury), Hawkesbury 1797, Australia. j.rupesinghe@westernsydney.edu.au.
authors.forename: Emmanuel Janaka Rochana
authors.initials: EJ
authors.lastname: Rupesinghe
authors.affiliation: Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia. 18209352@student.uws.edu.au.
authors.forename: Andrew
authors.initials: A
authors.lastname: Jones
authors.affiliation: Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia. r.shalliker@westernsydney.edu.au.
authors.forename: Ross Andrew
authors.initials: RA
authors.lastname: Shalliker
authors.affiliation: Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia. s.pravadali-cekic@westernsydney.edu.au.
authors.forename: Sercan
authors.initials: S
authors.lastname: Pravadali-Cekic
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010945:Plants, Edible / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts
keywords: active flow technology (AFT); antioxidants; cinnamon myrtle; high performance liquid chromatography (HPLC); lemon myrtle; multiplexed detection; native Australian food plants; post-column derivatisation (PCD)