title: Blue 'turn-on' fluorescent probes for the direct detection of free radicals and nitric oxide in Pseudomonas aeruginosa biofilms.
abstract: Two novel cell-permeable and bacteria-compatible 'turn-on' fluorescent probes, designed to be compatible in a multi-dye system and to fluoresce in the blue region exhibiting emission maxima of 440-490 nm, were synthesized. The profluorescent nitroxide probe (11) was developed to visualize and quantify free radical and redox processes, and the Cu(II)-complexed coumarin-based probe (16a) was developed for NO detection. Confocal laser-scanning microscopy and subsequent digital analysis of Pseudomonas aeruginosa biofilms stained with 11 or 16a determined that free radical and redox processes and NO generation occur predominantly in live cells during normal biofilm growth.
authors.affiliation: ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Australia.
authors.forename: Mina
authors.initials: M
authors.lastname: Barzegar Amiri Olia
authors.forename: Athanasios
authors.initials: A
authors.lastname: Zavras
authors.forename: Carl H
authors.initials: CH
authors.lastname: Schiesser
authors.forename: Stefanie-Ann
authors.initials: SA
authors.lastname: Alexander
mesh_terms: D018441:Biofilms / Q000254:growth & development; D002463:Cell Membrane Permeability; D003300:Copper / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000145:classification / Q000493:pharmacokinetics*; D005609:Free Radicals / Q000032:analysis*; D018613:Microscopy, Confocal; D009569:Nitric Oxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa; D013194:Staining and Labeling
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D005609:Free Radicals; D009569:Nitric Oxide; D003300:Copper; C030123:coumarin