title: Stable-Isotope Probing RNA to Study Plant/Fungus Interactions.
abstract: The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable-isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plant and its associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts.
authors.affiliation: UMR 6553 Ecobio, CNRS, Université de Rennes 1, campus Beaulieu, Rennes, 35000, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Lê Van
authors.affiliation: UMR 6553 Ecobio, CNRS, Université de Rennes 1, campus Beaulieu, Rennes, 35000, France.|Department of biology, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Marie
authors.initials: M
authors.lastname: Duhamel
authors.affiliation: UMR 6553 Ecobio, CNRS, Université de Rennes 1, campus Beaulieu, Rennes, 35000, France.
authors.forename: Achim
authors.initials: A
authors.lastname: Quaiser
authors.affiliation: UMR 6553 Ecobio, CNRS, Université de Rennes 1, campus Beaulieu, Rennes, 35000, France. philippe.vandenkoornhuyse@univ-rennes1.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Vandenkoornhuyse
mesh_terms: D007553:Isotope Labeling / Q000379:methods*; D038821:Mycorrhizae / Q000235:genetics* / Q000302:isolation & purification; D010944:Plants / Q000235:genetics / Q000382:microbiology; D015347:RNA Probes / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D015347:RNA Probes; D012335:RNA, Ribosomal
keywords: Carbon thirteen; Carbon transfer; Fungal plant symbiont; RNA; Stable-isotope probing (SIP); qRT-PCR