title: First isolation and antinociceptive activity of a lipid transfer protein from noni (Morinda citrifolia) seeds.
abstract: In this study a novel heat-stable lipid transfer protein, designated McLTP1, was purified from noni (Morinda citrifolia L.) seeds, using four purification steps which resulted in a high-purified protein yield (72 mg McLTP1 from 100g of noni seeds). McLTP1 exhibited molecular masses of 9.450 and 9.466 kDa, determined by electrospray ionisation mass spectrometry. The N-terminal sequence of McLTP1 (AVPCGQVSSALSPCMSYLTGGGDDPEARCCAGV), as analysed by NCBI-BLAST database, revealed a high degree of identity with other reported plant lipid transfer proteins. In addition, this protein proved to be resistant to pepsin, trypsin and chymotrypsin digestion. McLTP1 given intraperitoneally (1, 2, 4 and 8 mg/kg) and orally (8 mg/kg) caused an inhibition of the writhing response induced by acetic acid in mice. This protein displayed thermostability, retaining 100% of its antinociceptive activity after 30 min incubation at 80 °C. Pretreatment of mice with McLTP1 (8 mg/kg, i.p. and p.o.) also decreased neurogenic and inflammatory phases of nociception in the formalin test. Naloxone (2 mg/kg, i.p.) antagonised the antinociceptive effect of McLTP1 suggesting that the opioid mechanisms mediate the analgesic properties of this protein.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil.
authors.forename: Dyély C O
authors.initials: DC
authors.lastname: Campos
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil.
authors.forename: Andrea S
authors.initials: AS
authors.lastname: Costa
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil.
authors.forename: Amanda D R
authors.initials: AD
authors.lastname: Lima
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil.
authors.forename: Fredy D A
authors.initials: FD
authors.lastname: Silva
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil.
authors.forename: Marina D P
authors.initials: MD
authors.lastname: Lobo
authors.affiliation: NUBEX-Núcleo de Biologia Experimental, Centro de Ciências da Saúde, Universidade de Fortaleza-UNIFOR, 60811-905 Fortaleza, CE, Brazil.
authors.forename: Ana Cristina O
authors.initials: AC
authors.lastname: Monteiro-Moreira
authors.affiliation: NUBEX-Núcleo de Biologia Experimental, Centro de Ciências da Saúde, Universidade de Fortaleza-UNIFOR, 60811-905 Fortaleza, CE, Brazil.
authors.forename: Renato A
authors.initials: RA
authors.lastname: Moreira
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Luzia K A M
authors.initials: LK
authors.lastname: Leal
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Diogo
authors.initials: D
authors.lastname: Miron
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil.
authors.forename: Ilka M
authors.initials: IM
authors.lastname: Vasconcelos
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, 60440-900 Fortaleza, CE, Brazil. Electronic address: hermogenes@ufc.br.
authors.forename: Hermógenes D
authors.initials: HD
authors.lastname: Oliveira
mesh_terms: D000595:Amino Acid Sequence; D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D052179:Antigens, Plant / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D008297:Male; D051379:Mice; D032066:Morinda / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012018:Reflex / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*
chemical_list: D000700:Analgesics; D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C511920:lipid transfer proteins, plant
keywords: Antinociceptive activity; Lipid transfer protein; Morinda citrifolia L.; Noni; Protein isolation; Rubiaceae