title: Effects of Collagen Grafting on Cell Behaviors in BCP Scaffold with Interconnected Pore Structure.
abstract: BACKGROUND
  This study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation. Porous BCP scaffolds with interconnected micropore structure were prepared with were prepared and then grafted with a collagen type I. The hydroxyapatite (HA) and β-tricalcium phosphate (TCP) ratio of the TCP scaffolds was about 60/40 and the collagen was crosslinked on the TCP scaffold surface (collagen-TCP).
  
  
  RESULTS
  The sintered BCP scaffolds showed fully interconnected micropore structures with submicron-sized grains. The collagen crosslinking in the scaffolds was conducted using the the N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) crosslinking method. The cell proliferation of collagen-BCP scaffolds showed a similar result to that of the BCP scaffolds. However, osteoblastic differentiation and cell attachment increased in the collagen-BCP scaffolds.
  
  
  CONCLUSIONS
  Collagen-BCP scaffold improved the cell attachment ability in early phase and osteoblastic differentiation.
authors.affiliation: Department of Institute of Science & Technology, Megagen Implant, Jain-myeon, Gyeongsan, Gyeongbuk 712-852 Korea.
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Yang
authors.affiliation: School of Materials Science & Engineering, Yeungnam University, Gyeongsan, Gyeongbuk 712-749 Korea.
authors.forename: Jae-Hui
authors.initials: JH
authors.lastname: Jeon
authors.affiliation: Department of Institute of Science & Technology, Megagen Implant, Jain-myeon, Gyeongsan, Gyeongbuk 712-852 Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Institute of Science & Technology, Megagen Implant, Jain-myeon, Gyeongsan, Gyeongbuk 712-852 Korea.
authors.forename: Hyun-Wook
authors.initials: HW
authors.lastname: An
authors.affiliation: Department of Institute of Science & Technology, Megagen Implant, Jain-myeon, Gyeongsan, Gyeongbuk 712-852 Korea.
authors.forename: Keun Oh
authors.initials: KO
authors.lastname: Park
authors.affiliation: Department of Institute of Science & Technology, Megagen Implant, Jain-myeon, Gyeongsan, Gyeongbuk 712-852 Korea.
authors.forename: Kwang-Bum
authors.initials: KB
authors.lastname: Park
authors.affiliation: School of Materials Science & Engineering, Yeungnam University, Gyeongsan, Gyeongbuk 712-749 Korea.
authors.forename: Sukyoung
authors.initials: S
authors.lastname: Kim
keywords: Biphasic calcium phosphate (BCP); Collagen; Hydroxyapatite (HA); Tricalcium phosphate (TCP)