title: Abca7 deletion does not affect adult neurogenesis in the mouse.
abstract: ATP-binding cassette transporter A7 (ABCA7) is highly expressed in the brain. Recent genome-wide association studies (GWAS) have identified ABCA7 single nucleotide polymorphisms (SNPs) that increase Alzheimer's disease (AD) risk, however, the mechanisms by which ABCA7 may control AD risk remain to be fully elucidated. Based on previous research suggesting that certain ABC transporters may play a role in the regulation of neurogenesis, we conducted a study of cell proliferation and neurogenic potential using cellular bromodeoxyuridine (BrdU) incorporation and doublecortin (DCX) immunostaining in adult Abca7 deficient mice and wild-type-like (WT) littermates. In the present study counting of BrdU-positive and DCX-positive cells in an established adult neurogenesis site in the dentate gyrus (DG) indicated there were no significant differences when WT and Abca7 deficient mice were compared. We also measured the area occupied by immunohistochemical staining for BrdU and DCX in the DG and the subventricular zone (SVZ) of the same mice and this confirmed that ABCA7 does not play a significant role in the regulation of cell proliferation or neurogenesis in the adult mouse.
authors.affiliation: Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Li
authors.affiliation: Neuroscience Research Australia, Randwick, NSW 2031, Australia School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia Schizophrenia Research Institute, Randwick, NSW 2031, Australia.
authors.forename: Tim
authors.initials: T
authors.lastname: Karl
authors.affiliation: Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia brettg@uow.edu.au.
authors.forename: Brett
authors.initials: B
authors.lastname: Garner
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000172:deficiency* / Q000378:metabolism; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000378:metabolism* / Q000473:pathology; D000089662:Doublecortin Protein; D020547:Lateral Ventricles / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D055495:Neurogenesis; D020641:Polymorphism, Single Nucleotide
chemical_list: D018528:ATP-Binding Cassette Transporters; C485856:Abca7 protein, mouse; C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein
keywords: ABCA7; Alzheimer's disease; bromodeoxyuridine; dentate gyrus; doublecortin; neurogenesis; subventricular zone