title: Psychosocial determinants of diurnal alpha-amylase among healthy Quebec workers.
abstract: Salivary alpha-amylase (sAA) is a stress-sensitive biomarker the shows promise as an indirect proxy of sympathetic-adrenal-medullary axis activities that are otherwise difficult to discern non-invasively. This comprehensive study investigated diurnal sAA in association with numerous psychosocial characteristics related to mental health, work stress, and non-work stress. Participants included 395 workers (56.1% women, age: M=41.3, SD=10.81) from across 34 distinct workplaces. Diurnal sAA was sampled over two non-consecutive work days at awakening, 30 min after awakening, 14h00, 16h00, and bedtime. Well-validated psychometrics and survey items were used to measure mental health (psychological distress, depression, burnout, work characteristics) (task design, demands, social relations, gratifications), and non-work characteristics (marital/parental status, economic statuses, marital and parental stress, work-family conflicts). Preliminary results revealed that men showed occasionally higher sAA concentrations than women. Multilevel regressions were used to analyze sAA concentrations nested according to levels (i) for each time-point, (ii) between workers, and (iii) across workplaces while covarying for time of awakening, sex, age, cigarette smoking, alcohol consumption, regular physical activity, psychotropic drug use, and body mass index. Main results revealed that psychological demands, support from colleagues, interpersonal conflicts, job recognition and job insecurity appear to be associated with diurnal sAA, while non-work factors did not. Our findings showing a distinct diurnal profile for sAA replicate and expand those of Nater et al. (2007, Psychoneuroendocrinology 32, 392-401), providing further evidence that sAA is associated to subjective psychosocial factors.
authors.affiliation: School of Industrial Relations, University of Montreal, C.P. 6128, Succ Centre-Ville, Montreal, Quebec H3C 3J7, Canada. Electronic address: alain.marchand@umontreal.ca.
authors.forename: Alain
authors.initials: A
authors.lastname: Marchand
authors.affiliation: Integrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.
authors.forename: Robert-Paul
authors.initials: RP
authors.lastname: Juster
authors.affiliation: Department of Psychiatry, University of Montreal, Montreal, Quebec, Canada.
authors.forename: Sonia J
authors.initials: SJ
authors.lastname: Lupien
authors.affiliation: School of Industrial Relations, University of Montreal, C.P. 6128, Succ Centre-Ville, Montreal, Quebec H3C 3J7, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Durand
mesh_terms: D000328:Adult; D002940:Circadian Rhythm; D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D008875:Middle Aged; D016272:Occupational Health; D011792:Quebec / Q000453:epidemiology; D055291:Salivary alpha-Amylases / Q000378:metabolism*; D013315:Stress, Psychological / Q000378:metabolism / Q000523:psychology; D014937:Work / Q000523:psychology / Q000706:statistics & numerical data; D017132:Workplace / Q000523:psychology* / Q000706:statistics & numerical data*
chemical_list: D055291:Salivary alpha-Amylases
keywords: Diurnal variation; Mental health; Salivary alpha-amylase; Stress biomarkers; Work and non-work stress