title: Analysis of the in vivo proliferative capacity of a whole cell cancer vaccine.
abstract: A polyvalent therapeutic cancer cell vaccine containing three viable, irradiated, replication-incompetent melanoma cell lines (Canvaxin) was administered to over 2500 patients in various clinical studies. This study examines the fate of Canvaxin cells in 16 patients with Stage II, III or IV melanoma, with special attention on assessing the capacity of the vaccine cells to replicate. The survival time and the potential proliferative capacity of irradiated Canvaxin cells in humans was studied by histologic examination of biopsies of injection sites over a two-week period. The overall results show that the vaccine cell mitotic index in skin biopsies (0.12%) was approximately 6 times lower than the control value (0.71%). Similarly, there was an overall trend toward a decrease in mitotic figures during the two week observation periods. Also, there was a trend towards a decrease in the number of vaccine cells and mitotic figures with increasing cycles. The data indicate that Canvaxin cells do not proliferate after intra-dermal injection, and that cells typically associated with an immune response are present at the inoculation sites.
authors.affiliation: CancerVax Corp., Carlsbad, CA, USA. Electronic address: jpmdpsca@aol.com.
authors.forename: John
authors.initials: J
authors.lastname: Petricciani
authors.affiliation: CancerVax Corp., Carlsbad, CA, USA.
authors.forename: Eugene
authors.initials: E
authors.lastname: Koren
authors.affiliation: John Wayne Cancer Institute, Santa Monica, CA, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Morton
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D019496:Cancer Vaccines / Q000008:administration & dosage; D002453:Cell Cycle / Q000187:drug effects; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology
chemical_list: D019496:Cancer Vaccines; C464842:Canvaxin
keywords: Cancer; Cell division; Cell substrate; Melanoma; Vaccine