title: Processes for design, construction and utilisation of arrays of light-emitting diodes and light-emitting diode-coupled optical fibres for multi-site brain light delivery.
abstract: Optogenetics enables light to be used to control the activity of genetically targeted cells in the living brain. Optical fibers can be used to deliver light to deep targets, and LEDs can be spatially arranged to enable patterned light delivery. In combination, arrays of LED-coupled optical fibers can enable patterned light delivery to deep targets in the brain. Here we describe the process flow for making LED arrays and LED-coupled optical fiber arrays, explaining key optical, electrical, thermal, and mechanical design principles to enable the manufacturing, assembly, and testing of such multi-site targetable optical devices. We also explore accessory strategies such as surgical automation approaches as well as innovations to enable low-noise concurrent electrophysiology.
authors.affiliation: The MIT Media Laboratory, Synthetic Neurobiology Group, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.; Department of Brain and Cognitive Sciences and MIT McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Jacob G
authors.initials: JG
authors.lastname: Bernstein
authors.affiliation: The MIT Media Laboratory, Synthetic Neurobiology Group, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.; Department of Brain and Cognitive Sciences and MIT McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Allen
authors.affiliation: The MIT Media Laboratory, Synthetic Neurobiology Group, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.; Department of Brain and Cognitive Sciences and MIT McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Guerra
authors.affiliation: The MIT Media Laboratory, Synthetic Neurobiology Group, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.; Department of Brain and Cognitive Sciences and MIT McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Edward S
authors.initials: ES
authors.lastname: Boyden