title: Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement.
abstract: Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University , 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshikawa
authors.affiliation: Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University , 1-1-1, Tsushima-Naka, Kita-ku, Okayama, 700-8530, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Furuta
authors.affiliation: Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University , 1-1-1, Tsushima-Naka, Kita-ku, Okayama, 700-8530, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University , 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
mesh_terms: D015550:Cell Degranulation; D002460:Cell Line; D003035:Cobalt / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006632:Histamine / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000379:methods*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D003035:Cobalt; D006632:Histamine