title: How Do the Size, Charge and Shape of Nanoparticles Affect Amyloid β Aggregation on Brain Lipid Bilayer?
abstract: Here, we studied the effect of the size, shape, and surface charge of Au nanoparticles (AuNPs) on amyloid beta (Aβ) aggregation on a total brain lipid-based supported lipid bilayer (brain SLB), a fluid platform that facilitates Aβ-AuNP aggregation process. We found that larger AuNPs induce large and amorphous aggregates on the brain SLB, whereas smaller AuNPs induce protofibrillar Aβ structures. Positively charged AuNPs were more strongly attracted to Aβ than negatively charged AuNPs, and the stronger interactions between AuNPs and Aβ resulted in fewer β-sheets and more random coil structures. We also compared spherical AuNPs, gold nanorods (AuNRs), and gold nanocubes (AuNCs) to study the effect of nanoparticle shape on Aβ aggregation on the brain SLB. Aβ was preferentially bound to the long axis of AuNRs and fewer fibrils were formed whereas all the facets of AuNCs interacted with Aβ to produce the fibril networks. Finally, it was revealed that different nanostructures induce different cytotoxicity on neuroblastoma cells, and, overall, smaller Aβ aggregates induce higher cytotoxicity. The results offer insight into the roles of NPs and brain SLB in Aβ aggregation on the cell membrane and can facilitate the understanding of Aβ-nanostructure co-aggregation mechanism and tuning Aβ aggregate structures.
authors.affiliation: Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea.
authors.forename: Yuna
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea.
authors.forename: Hyojin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea.
authors.forename: Jwa-Min
authors.initials: JM
authors.lastname: Nam
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D009447:Neuroblastoma / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D008051:Lipid Bilayers; D010446:Peptide Fragments; D006046:Gold