title: Yeast β-d-glucans induced antimicrobial peptide expressions against Salmonella infection in broiler chickens.
abstract: The present study was designed to investigate the effects of yeast β-d-glucans (YG) on gene expression of endogenous β-defensins (AvBDs), cathelicidins (Cath) and liver-expressed antimicrobial peptide-2 (LEAP-2) in broilers challenged with Salmonella enteritidis (SE). 240 day-old Cobb male broilers were randomly assigned to 2×2 factorial arrangements of treatments with two levels of dietary YG (0 or 200mg/kg in diet) and two levels of SE challenge (0 or 1×10(9) SE at 7-9 days of age). The results showed SE infection reduced growth performance,and increased salmonella cecal colonization and internal organs invasion, increased concentration of intestinal specific IgA and serum specific IgG antibody, as compared to uninfected birds. SE challenge differentially regulated AvBDs, Caths and LEAP-2 gene expression in the jejunum and spleen of broiler chickens during the infection period. However, YG supplementation inhibited the growth depression by SE challenge, and further increased level of serum specific IgG and intestinal specific IgA antibody. Higher level of salmonella colonization and internal organs invasion in the SE-infected birds were reduced by YG. SE-induced differentially expression patterns of AMPs genes was inhibited or changed by YG. Results indicated YG enhance chicken's resistance to salmonella infection.
authors.affiliation: College of Biology, China Agricultural University, Beijing, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address: wangzh@cau.edu.cn.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Angel Yeast Co., Ltd., Yichang City, Hubei, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000494:pharmacology*; D002645:Chickens; D015870:Gene Expression; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D011201:Poultry Diseases / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology*; D012475:Salmonella / Q000187:drug effects* / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology*; D015003:Yeasts / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000907:Antibodies, Bacterial; D023181:Antimicrobial Cationic Peptides; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D047071:beta-Glucans
keywords: Antimicrobial peptides; Salmonella enteritidis; Yeast β-d-glucans