title: Small angle X-ray scattering data and structure factor fitting for the study of the quaternary structure of the spermidine N-acetyltransferase SpeG.
abstract: Here we describe the treatment of the small-angle X-ray Scattering (SAXS) data used during SpeG quaternary structure study as part of the research article "Substrate induced allosteric change in the quaternary structure of the spermidine N-acetyltransferase SpeG" published in Journal of Molecular Biology [1]. These data were collected on two separate area detectors as separate dilution series of the SpeG and the SpeG with spermine samples along with data from their companion buffers. The data were radially integrated, corrected for incident beam variation, and scaled to absolute units. After subtraction of volume-fraction scaled buffer scattering and division by the SpeG concentration, multiple scattering curves free of an inter-molecular structure factor were derived from the dilution series. Rather than extrapolating to infinite dilution, the structure factor contribution was estimated by fitting to the full set of data provided by dividing the scattering curves of a dilution series by the curve from the most dilute sample in that series.
authors.affiliation: DND-CAT Synchrotron Research Center, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Weigand
authors.affiliation: Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine, Department of Biochemistry and Molecular Genetics, Chicago, IL 60611, USA.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Filippova
authors.affiliation: Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine, Department of Biochemistry and Molecular Genetics, Chicago, IL 60611, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Kiryukhina
authors.affiliation: Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine, Department of Biochemistry and Molecular Genetics, Chicago, IL 60611, USA.
authors.forename: Wayne F
authors.initials: WF
authors.lastname: Anderson