title: Interleukin-10 deficiency aggravates angiotensin II-induced cardiac remodeling in mice.
abstract: OBJECTIVE
  This study examined the role of interleukin (IL)-10 in angiotensin II-induced cardiac remodeling.
  
  
  METHODS
  Angiotensin II was infused subcutaneously (1.1mg/kg/day) for one week in IL-10 knockout and wild-type mice, after which cardiac function and hypertrophy were assessed by echocardiogram.
  
  
  RESULTS
  IL-10 gene expression in the heart was increased by angiotensin II infusion. Plasma levels of brain natriuretic peptide (BNP) and gene expression of BNP in the heart were increased by IL-10 deficiency or angiotensin II, and plasma BNP levels were further increased by IL-10 deficiency with angiotensin II. IL-10 deficiency increased the left ventricular dimension, whereas treatment with angiotensin II increased heart weight. Angiotensin II significantly reduced cardiac function in IL-10 knockout mice compared with wild-type mice. Gene expression of tumor necrosis factor-α and interleukin-6 was increased by IL-10 deficiency or angiotensin II infusion, and these increases were further enhanced by IL-10 deficiency with angiotensin II. Gene expression of collagen I/III and collagen III protein levels were increased by angiotensin II but not by IL-10 deficiency. Gene expression of matrix metalloproteinase2/9 was increased by IL-10 deficiency or angiotensin II, and this expression was further increased by IL-10 deficiency with angiotensin II. Akt phosphorylation was increased by IL-10 deficiency or angiotensin II and further increased by IL-10 deficiency with angiotensin II. Phosphorylation of p38 was increased by IL-10 deficiency.
  
  
  CONCLUSIONS
  These results suggest that IL-10 deficiency causes deterioration in cardiac functions in angiotensin II-infused mice, suggesting that IL-10 plays a protective role against angiotensin II-induced cardiac remodeling.
authors.affiliation: Department of Physiology, College of Medicine, Yeungnam University, South Korea.
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Kwon
authors.affiliation: Department of Physiology, College of Medicine, Yeungnam University, South Korea.
authors.forename: Hye-Na
authors.initials: HN
authors.lastname: Cha
authors.affiliation: Department of Physiology, College of Medicine, Yeungnam University, South Korea.
authors.forename: Jung-Yoon
authors.initials: JY
authors.lastname: Heo
authors.affiliation: Department of Internal Medicine, Pusan National University Hospital, Pusan, South Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of internal Medicine, College of Medicine, Yeungnam University, Daegu, South Korea.
authors.forename: Byung Ik
authors.initials: BI
authors.lastname: Jang
authors.affiliation: Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, South Korea.
authors.forename: In-Kye
authors.initials: IK
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Medicine, Yeungnam University, South Korea. Electronic address: sypark@med.yu.ac.kr.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
mesh_terms: D000804:Angiotensin II / Q000633:toxicity*; D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000235:genetics*; D003094:Collagen / Q000096:biosynthesis; D016753:Interleukin-10 / Q000172:deficiency* / Q000235:genetics*; D015850:Interleukin-6 / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000378:metabolism; D051058:Oncogene Protein v-akt / Q000096:biosynthesis / Q000235:genetics; D014463:Ultrasonography; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: C556062:IL10 protein, mouse; D015850:Interleukin-6; C577686:interleukin-6, mouse; D000804:Angiotensin II; D020097:Natriuretic Peptide, Brain; D016753:Interleukin-10; D003094:Collagen; D051058:Oncogene Protein v-akt; D020782:Matrix Metalloproteinases
keywords: Angiotensin II; Cytokines; Heart; Interleukin-10