title: Effect of Acetamizuril on enolase in second-generation merozoites of Eimeria tenella.
abstract: As an obligate intracellular apicomplexan parasite, Eimeria tenella (E. tenella) can rapidly invade chicken cecum epithelial cells and cause avian coccidiosis. Enolase, an essential enzyme that catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate, plays a very important role in glycolysis. In this study, each chicken was inoculated with 8×10(4) sporulated E. tenella oocysts suspended in 1ml of distilled water to determine the effects of acetamizuril, a new triazine anticoccidial drug, on enolase in the second-generation merozoites of E. tenella. The chickens were divided into two groups: the untreatment group (challenged with E. tenella oocysts and provided with normal feed) and the treatment group (challenged with E. tenella oocysts and provided with 5mg/kg of acetamizuril by oral gavage at 96h after inoculation). The second-generation merozoites of E. tenella (mz-En) were obtained at 120h after inoculation. Subsequently, quantitative real-time PCR and Western blotting were conducted to detect the enolase changes in mz-En at the transcriptional and translational levels. The results showed that enolase mRNA expression was downregulated, and the translational level was decreased in the treatment group. In addition, the subcellular localization of enolase demonstrated that enolase was distributed primarily at the top of the mz-En and that the fluorescence intensity was weak after treatment with acetamizuril. These findings indicated that enolase may be a promising target to prevent coccidiosis.
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Zhao-guo
authors.initials: ZG
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Rong-sheng
authors.initials: RS
authors.lastname: Mi
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Ke-yu
authors.initials: KY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China.
authors.forename: Chen-zhong
authors.initials: CZ
authors.lastname: Fei
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China. Electronic address: fqxue@shvri.ac.cn.
authors.forename: Fei-qun
authors.initials: FQ
authors.lastname: Xue
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, PR China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai 200241, People's Republic of China. Electronic address: litao@shvri.ac.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D003049:Coccidiostats / Q000494:pharmacology*; D016786:Eimeria tenella / Q000187:drug effects* / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008297:Male; D052940:Merozoites / Q000187:drug effects* / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism*; D011201:Poultry Diseases / Q000469:parasitology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics; D014227:Triazines / Q000494:pharmacology*
chemical_list: D003049:Coccidiostats; D012333:RNA, Messenger; D016053:RNA, Protozoan; D014227:Triazines; D010751:Phosphopyruvate Hydratase
keywords: Acetamizuril; E. tenella; Enolase; Second-generation merozoites