title: Optimization of Culture Conditions for Fermentation of Soymilk Using Lactobacillus casei by Response Surface Methodology.
abstract: Soymilk was fermented with Lactobacillus casei, and statistical experimental design was used to investigate factors affecting viable cells of L. casei, including temperature, glucose, niacin, riboflavin, pyridoxine, folic acid and pantothenic acid. Initial screening by Plackett-Burman design revealed that among these factors, temperature, glucose and niacin have significant effects on the growth of L. casei. Further optimization with Box-Behnken design and response surface analysis showed that a second-order polynomial model fits the experimental data appropriately. The optimum conditions for temperature, glucose and niacin were found to be 15.77 °C, 5.23 and 0.63 g/L, respectively. The concentration of viable L. casei cells under these conditions was 8.23 log10 (CFU/mL). The perfect agreement between the observed values and the values predicted by the equation confirms the statistical significance of the model and the model's adequate precision in predicting optimum conditions.
authors.affiliation: Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155-6451, Tehran, Iran.
authors.forename: Feriyar
authors.initials: F
authors.lastname: Khoshayand
authors.affiliation: Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155-6451, Tehran, Iran.|Department of Obstetrics and Gynecology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Goodarzi
authors.affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155-6451, Tehran, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Shahverdi
authors.affiliation: Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155-6451, Tehran, Iran. khoshayand@tums.ac.ir.|Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, 14155-6451, Tehran, Iran. khoshayand@tums.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Khoshayand
keywords: Box-Behnken; Fermentation; Plackett-Burman; Probiotics; Response surface methodology; Soymilk