title: Quantitative analysis of serotonin secreted by human embryonic stem cells-derived serotonergic neurons via pH-mediated online stacking-CE-ESI-MRM.
abstract: A CE-ESI-MRM-based assay was developed for targeted analysis of serotonin released by human embryonic stem cells-derived serotonergic neurons in a chemically defined environment. A discontinuous electrolyte system was optimized for pH-mediated online stacking of serotonin. Combining with a liquid-liquid extraction procedure, LOD of serotonin in the Krebs'-Ringer's solution by CE-ESI-MS/MS on a 3D ion trap MS was0.15 ng/mL. The quantitative results confirmed the serotonergic identity of the in vitro developed neurons and the capacity of these neurons to release serotonin in response to stimulus.
authors.affiliation: School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Zhong
authors.affiliation: School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of Neuroscience and Department of Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, WI, USA.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Lu
authors.affiliation: China Pharmaceutical University, Nanjing, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Neuroscience and Department of Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, WI, USA.
authors.forename: Su-Chun
authors.initials: SC
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.|Department of Chemistry, University of Wisconsin, Madison, WI, USA.|School of Life Sciences, Tianjin University, Tianjin, P. R. China.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D002460:Cell Line; D019075:Electrophoresis, Capillary / Q000379:methods*; D000066449:Human Embryonic Stem Cells / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059625:Liquid-Liquid Extraction; D012701:Serotonin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D012701:Serotonin
keywords: CE-ESI-MRM; Online concentration; Serotonergic neuron; Serotonin