title: miR-625 suppresses cell proliferation and migration by targeting HMGA1 in breast cancer.
abstract: Dysregulation of microRNA contributes to the high incidence and mortality of breast cancer. Here, we show that miR-625 was frequently down-regulated in breast cancer. Decrease of miR-625 was closely associated with estrogen receptor (P = 0.004), human epidermal growth factor receptor 2 (P = 0.003) and clinical stage (P = 0.001). Kaplan-Meier and multivariate analyses indicated miR-625 as an independent factor for unfavorable prognosis (hazard ratio = 2.654, 95% confident interval: 1.300-5.382, P = 0.007). Re-expression of miR-625 impeded, whereas knockdown of miR-625 enhanced cell viabilities and migration abilities in breast cancer cells. HMGA1 was confirmed as a direct target of miR-625. The expressions of HMGA1 mRNA and protein were induced by miR-625 mimics, but reduced by miR-625 inhibitor. Re-introduction of HMGA1 in cells expressing miR-625 distinctly abrogated miR-625-mediated inhibition of cell growth. Taken together, our data demonstrate that miR-625 suppresses cell proliferation and migration by targeting HMGA1 and suggest miR-625 as a promising prognostic biomarker and a potential therapeutic target for breast cancer.
authors.affiliation: Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Zhou
authors.affiliation: Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Cai-neng
authors.initials: CN
authors.lastname: Zhong
authors.affiliation: Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Xun-peng
authors.initials: XP
authors.lastname: Luo
authors.affiliation: Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Ya-yuan
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China. Electronic address: 1072241978@qq.com.
authors.forename: Dong-xian
authors.initials: DX
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary and Pancreas Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China. Electronic address: leoliping@aliyun.com.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D002681:China / Q000453:epidemiology; D005260:Female; D025741:HMGA1a Protein / Q000378:metabolism*; D006801:Humans; D015994:Incidence; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D012307:Risk Factors; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C575476:MIRN625 microRNA, human; D035683:MicroRNAs; D025741:HMGA1a Protein
keywords: Breast cancer; HMGA1; Migration; Proliferation; miR-625