title: Identification of an interaction between EI and a histidine kinase-response regulator hybrid protein in Gluconobacter oxydans.
abstract: Gluconobacter oxydans may contain an incomplete phosphoenolpyruvate: carbohydrate phosphotransferase system consisting of three components--EI, HPr and EIIA, while the function of individual members of the system remains unknown. In this research, a specific interaction between EI and a histidine kinase-response regulator hybrid protein was screened by yeast two-hybrid assay, and the interaction was further identified with GST pull-down assay and bimolecular fluorescence complementation assay in vitro and in vivo, respectively. As the histidine kinase-response regulator hybrid protein serves as a member of two-component system in G. oxydans, its interaction with EI implied that PTS may play certain roles in bacteria under stress.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China. Electronic address: myushu@ecust.edu.cn.
authors.forename: Yushu
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000502:physiology*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D000071677:Histidine Kinase; D010770:Phosphotransferases / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010770:Phosphotransferases; D011494:Protein Kinases; D000071677:Histidine Kinase
keywords: Bimolecular fluorescence complementation; GST pull-down; Gluconobacter oxydans; Phosphoenolpyruvate: carbohydrate phosphotransferase system; Two-component system; Yeast two-hybrid assay