title: Metagenomic analysis of a desulphurisation system used to treat biogas from vinasse methanisation.
abstract: We investigated the response of microbial community to changes in H2S loading rate in a microaerated desulphurisation system treating biogas from vinasse methanisation. H2S removal efficiency was high, and both COD and DO seemed to be important parameters to biomass activity. DGGE analysis retrieved sequences of sulphide-oxidising bacteria (SOB), such as Thioalkalimicrobium sp. Deep sequencing analysis revealed that the microbial community was complex and remained constant throughout the experiment. Most sequences belonged to Firmicutes and Proteobacteria, and, to a lesser extent, Bacteroidetes, Chloroflexi, and Synergistetes. Despite the high sulphide removal efficiency, the abundance of the taxa of SOB was low, and was negatively affected by the high sulphide loading rate.
authors.affiliation: Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil.
authors.forename: Marcela França
authors.initials: MF
authors.lastname: Dias
authors.affiliation: Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil; Methanum Waste and Energy, Alameda do Ingá, 840, Room 908, Vale do Sereno, Nova Lima, Minas Gerais State 34.000-000, Brazil.
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Colturato
authors.affiliation: Methanum Waste and Energy, Alameda do Ingá, 840, Room 908, Vale do Sereno, Nova Lima, Minas Gerais State 34.000-000, Brazil.
authors.forename: João Paulo
authors.initials: JP
authors.lastname: de Oliveira
authors.affiliation: Genomics and Computational Biology Group, René Rachou Research Center, Oswaldo Cruz Foundation, Augusto de Lima Avenue, 1717, Belo Horizonte, Minas Gerais State 30.190-002, Brazil.
authors.forename: Laura Rabelo
authors.initials: LR
authors.lastname: Leite
authors.affiliation: Genomics and Computational Biology Group, René Rachou Research Center, Oswaldo Cruz Foundation, Augusto de Lima Avenue, 1717, Belo Horizonte, Minas Gerais State 30.190-002, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Oliveira
authors.affiliation: Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil.
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Chernicharo
authors.affiliation: Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil. Electronic address: juliana@desa.ufmg.br.
authors.forename: Juliana Calabria
authors.initials: JC
authors.lastname: de Araújo
mesh_terms: D001419:Bacteria; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D006862:Hydrogen Sulfide / Q000737:chemistry; D056186:Metagenomics; D059013:Microbial Consortia; D031786:Saccharum / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*
chemical_list: D056804:Biofuels; D013440:Sulfides; D006862:Hydrogen Sulfide
keywords: Biogas; DGGE; Desulphurisation; Metagenomics; Vinasse