title: Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on Antioxidant Assay and Chemical Profiles Combined with Multivariate Statistical Analysis.
abstract: An integrated approach including chemical and biological assessments was developed to investigate the differences between Apocynum venetum L. (AV) and its adulterant, Apocynum pictum Schrenk (AP). Ten flavonoids were tentatively identified by ultra-visible and mass spectra data. The chemical component, hyperoside, was identified as a critical parameter for discrimination of two species from the results of principal component analysis (PCA) and quantitative analysis. The anti-oxidative power of the herbal extracts were determined using 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay and H₂O₂-induced cell damage on LO2 cells. The results of the biological assays suggested that the chemical differences between AV and AP do lead to difference in activity and AV is demonstrated to have higher anti-oxidant activity.
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Shenzhen 518057, China. on.chan@polyu.edu.hk.|Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. on.chan@polyu.edu.hk.
authors.forename: Chi-On
authors.initials: CO
authors.lastname: Chan
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. christy.lau@connect.polyu.hk.
authors.forename: Ching-Ching
authors.initials: CC
authors.lastname: Lau
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. yam-fung@hotmail.com.
authors.forename: Yam-Fung
authors.initials: YF
authors.lastname: Ng
authors.affiliation: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China. xulijia@hotmail.com.
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Shenzhen 518057, China. sibao.chen@polyu.edu.hk.|Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. sibao.chen@polyu.edu.hk.|The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China. sibao.chen@polyu.edu.hk.
authors.forename: Si-Bao
authors.initials: SB
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Shenzhen 518057, China. sw.chan@polyu.edu.hk.|Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. sw.chan@polyu.edu.hk.
authors.forename: Shun-Wan
authors.initials: SW
authors.lastname: Chan
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Shenzhen 518057, China. daniel.mok@polyu.edu.hk.|Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. daniel.mok@polyu.edu.hk.
authors.forename: Daniel Kam-Wah
authors.initials: DK
authors.lastname: Mok
keywords: Apocynum pictum Schrenk; Apocynum venetum L.; HPLC fingerprints; free radical scavenging capacity