title: Hesperidin Attenuates Ultraviolet B-Induced Apoptosis by Mitigating Oxidative Stress in Human Keratinocytes.
abstract: Human skin cells undergo pathophysiological processes via generation of reactive oxygen species (ROS) upon excessive exposure to ultraviolet B (UVB) radiation. This study investigated the ability of hesperidin (C28H34O15) to prevent apoptosis due to oxidative stress generated through UVB-induced ROS. Hesperidin significantly scavenged ROS generated by UVB radiation, attenuated the oxidation of cellular macromolecules, established mitochondrial membrane polarization, and prevented the release of cytochrome c into the cytosol. Hesperidin downregulated expression of caspase-9, caspase-3, and Bcl-2-associated X protein, and upregulated expression of B-cell lymphoma 2. Hesperidin absorbed wavelengths of light within the UVB range. In summary, hesperidin shielded human keratinocytes from UVB radiation-induced damage and apoptosis via its antioxidant and UVB absorption properties.
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Susara Ruwan Kumara
authors.initials: SR
authors.lastname: Madduma Hewage
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Yea Seong
authors.initials: YS
authors.lastname: Ryu
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Xia
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Min Chang
authors.initials: MC
authors.lastname: Oh
authors.affiliation: Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea.
authors.forename: Uhee
authors.initials: U
authors.lastname: Jung
authors.affiliation: Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon 34057, Republic of Korea.|Department of Radiation Biotechnology and Applied Radioisotope, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: In Gyu
authors.initials: IG
authors.lastname: Kim
authors.affiliation: School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
keywords: Antioxidant; Apoptosis; Hesperidin; Reactive oxygen species; Ultraviolet B