title: Proteomic analysis of the TerC interactome: Novel links to tellurite resistance and pathogenicity.
abstract: The tellurite resistance gene operon (ter) is widely spread among bacterial species, particularly pathogenic species. The ter operon has been implicated in tellurite resistance, phage inhibition, colicine resistance, and pathogenicity. The TerC protein represents one of the key proteins in tellurite resistance and shows no significant homology to any protein of known function. So far, there is no experimental evidence for TerC interaction partners. In this study, proteomic-based methods, including blue native electrophoresis and co-immunoprecipitation combined with LC-MS/MS, have been used to identify TerC interaction partners and thus providing indirect evidence for tentative functions of TerC in Escherichia coli. An interactome has been constructed and robust physical interaction of integral membrane protein TerC with TerB, DctA, PspA, HslU, and RplK has been shown. The TerC-TerB complex appears to act as a central unit that may link different functional modules with biochemical activities of C4-dicarboxylate transport, inner membrane stress response (phage shock protein regulatory complex), ATPase/chaperone activity, and proteosynthesis. In previous reports, it was hypothesized that a transmembrane unit formed by TerC protein may interact with the TerD family, but herein neither TerD nor TerE proteins were identified as TerC complex components. We propose that TerD/TerE participates in tellurite resistance through TerC-independent action.
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria; Department of Molecular Biology, Faculty of Natural Science, Comenius University, Bratislava, Slovakia.
authors.forename: L
authors.initials: L
authors.lastname: Turkovicova
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Smidak
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Jung
authors.affiliation: Department of Molecular Biology, Faculty of Natural Science, Comenius University, Bratislava, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Turna
authors.affiliation: Department of Pharmaceutical Chemistry, University of Vienna, Vienna, Austria. Electronic address: gert.lubec@univie.ac.at.
authors.forename: G
authors.initials: G
authors.lastname: Lubec
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria. Electronic address: jana.aradska@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Aradska
mesh_terms: D024881:Drug Resistance, Bacterial / Q000187:drug effects* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D013691:Tellurium / Q000494:pharmacology*
chemical_list: D029968:Escherichia coli Proteins; C026660:tellurous acid; D013691:Tellurium
keywords: Bacteria; Blue native PAGE; Co-immunoprecipitation; LC–MS/MS; Tellurite resistance; TerC