title: Influence of Bxpel1 Gene Silencing by dsRNA Interference on the Development and Pathogenicity of the Pine Wood Nematode, Bursaphelenchus xylophilus.
abstract: As the causal agent of pine wilt disease (PWD), the pine wood nematode (PWN), Bursaphelenchus xylophilus, causes huge economic losses by devastating pine forests worldwide. The pectate lyase gene is essential for successful invasion of their host plants by plant-parasitic nematodes. To demonstrate the role of pectate lyase gene in the PWD process, RNA interference (RNAi) is used to analyze the function of the pectate lyase 1 gene in B. xylophilus (Bxpel1). The efficiency of RNAi was detected by real-time PCR. The result demonstrated that the quantity of B. xylophilus propagated with control solution treatment was 62 times greater than that soaking in double-stranded RNA (dsRNA) after B. xylophilus inoculation in Botrytis cinerea for the first generation (F1). The number of B. xylophilus soaking in control solution was doubled compared to that soaking in Bxpel1 dsRNA four days after inoculation in Pinus thunbergii. The quantity of B. xylophilus was reduced significantly (p < 0.001) after treatment with dsRNAi compared with that using a control solution treatment. Bxpel1 dsRNAi reduced the migration speed and reproduction of B. xylophilus in pine trees. The pathogenicity to P. thunbergii seedling of B. xylophilus was weaker after soaking in dsRNA solution compared with that after soaking in the control solution. Our results suggest that Bxpel1 gene is a significant pathogenic factor in the PWD process and this basic information may facilitate a better understanding of the molecular mechanism of PWD.
authors.affiliation: Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China. qiuxiuwen3@163.com.|Poyang Lake Eco-economy Research Center, Jiujiang University, Jiujiang 332005, China. qiuxiuwen3@163.com.
authors.forename: Xiu-Wen
authors.initials: XW
authors.lastname: Qiu
authors.affiliation: Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China. xqwu@njfu.edu.cn.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Wu
authors.affiliation: Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China. Lhuang@njfu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China. jrye@njfu.edu.cn.
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Ye
mesh_terms: D000818:Animals; D020868:Gene Silencing; D015801:Helminth Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D028223:Pinus / Q000469:parasitology*; D010935:Plant Diseases / Q000469:parasitology / Q000628:therapy; D011133:Polysaccharide-Lyases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D017187:Tylenchida / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D037521:Virulence Factors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014934:Wood / Q000469:parasitology
chemical_list: D015801:Helminth Proteins; D012330:RNA, Double-Stranded; D037521:Virulence Factors; D011133:Polysaccharide-Lyases; C023911:pectate lyase
keywords: Bursaphelenchus xylophilus; RNA interference (RNAi); pathogenicity; pectate lyase