title: Addicting diverse bacteria to a noncanonical amino acid.
abstract: Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Drew S
authors.initials: DS
authors.lastname: Tack
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Jared W
authors.initials: JW
authors.lastname: Ellefson
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Ross
authors.initials: R
authors.lastname: Thyer
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Gollihar
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Forster
authors.affiliation: Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D000596:Amino Acids / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D005075:Biological Evolution; D003062:Codon; D006090:Gram-Negative Bacteria / Q000235:genetics; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000737:chemistry; D015202:Protein Engineering; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000596:Amino Acids; D003062:Codon; D012329:RNA, Bacterial; D014443:Tyrosine; D010649:Phenylalanine; D012343:RNA, Transfer; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1