title: Structural Elucidation of a Carnosine-Acrolein Adduct and its Quantification in Human Urine Samples.
abstract: Aldehydes accumulate in inflammation, during myocardial infarction and have been associated with pain symptoms. One pathway of aldehyde detoxification is the conjugation with carnosine. A 3-methylpyridinium carnosine adduct from the reaction of carnosine and acrolein was characterized using extensive spectroscopic measurements. The adduct with urinary concentrations of 1.82 ± 0.68 nmol/mg of creatinine is one of the most abundant acrolein metabolites in urine and opens promising therapeutic strategies for carnosine.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Vanderson S
authors.initials: VS
authors.lastname: Bispo
authors.affiliation: Instituto de Ciências Exatas e Tecnologia, Universidade Paulista, São Paulo, São Paulo, Brazil.
authors.forename: Ivan P
authors.initials: IP
authors.lastname: de Arruda Campos
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Mascio
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Marisa H G
authors.initials: MH
authors.lastname: Medeiros
mesh_terms: D000171:Acrolein / Q000737:chemistry* / Q000652:urine*; D002336:Carnosine / Q000737:chemistry* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000171:Acrolein; D002336:Carnosine