title: MiR-572 prompted cell proliferation of human ovarian cancer cells by suppressing PPP2R2C expression.
abstract: Ovarian cancer (OC) remains one of the most common types of malignant cancer, and the molecular mechanism underlying its proliferation is still largely unclear. It is reported that microRNAs acted as important regulators of cell proliferation by regulating its targeted gene. In this study, our result showed that miR-572 was markedly upregulated in OC cell lines and clinical tissues. Results of both gain-of-function and loss-of-function experiments revealed that upregulation of miR-572 expression dramatically promoted OC cell proliferation, whereas decreased miR-572 expression significantly reduced cell proliferation. Bioinformatics analysis and luciferase reporter assays further revealed PPP2R2C, a putative tumor suppressor as a potential target of miR-572. Moreover, silencing of PPP2R2C using small interfering RNA (siRNA) counteracted the proliferation arrest by miR-572-in in OC cells. In sum, our data provide that miR-572 promoted cell proliferation in OC by targeting PPP2R2C and might serve as a therapeutic target of OC.
authors.affiliation: Institute of Gynecology and Obstetrics, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, People's Republic of China; Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Wu
authors.affiliation: Center for Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, People's Republic of China; Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China.
authors.forename: Yu-ling
authors.initials: YL
authors.lastname: Huang
authors.affiliation: Institute of Gynecology and Obstetrics, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, People's Republic of China.
authors.forename: Lan-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.affiliation: Institute of Gynecology and Obstetrics, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, People's Republic of China.
authors.forename: Geng
authors.initials: G
authors.lastname: Tian
authors.affiliation: Center for Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, People's Republic of China.
authors.forename: Qiong-Zhi
authors.initials: QZ
authors.lastname: Liao
authors.affiliation: Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China. Electronic address: shilingchendr@163.com.
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics*; D054648:Protein Phosphatase 2 / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C000603872:MIRN-572 microRNA, human; D035683:MicroRNAs; C517192:PPP2R2C protein, human; D054648:Protein Phosphatase 2
keywords: Cell proliferation; Ovarian cancer; PPP2R2C; miR-572