title: A facile method to in situ fabricate three dimensional gold nanoparticle micropatterns in a cell-resistant hydrogel.
abstract: A facile method for in situ fabrication of three-dimensional gold nanoparticle micropatterns in a cell-resistant polyethylene glycol hydrogel has been developed by combining photochemical synthesis of gold nanoparticles with photolithography technology. The gold nanoparticle micropatterns were further bio-modified with cell integrated polypeptide NcysBRGD based on a gold-thiol bond to improve cell behaviors. Primary cell tests showed that NcysBRGD can enhance cell adhesion very well on the surface of gold nanoparticle micropatterns.
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Hubei, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Ming-Hao
authors.initials: MH
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Zhao
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Xia
authors.forename: Rui-Mei
authors.initials: RM
authors.lastname: Jin
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055616:Microtechnology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D046888:Tissue Array Analysis / Q000295:instrumentation
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; D013438:Sulfhydryl Compounds; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006046:Gold