title: Pepino polyphenolic extract improved oxidative, inflammatory and glycative stress in the sciatic nerves of diabetic mice.
abstract: The effect of pepino polyphenolic extract (PPE) on diabetic neuropathy was examined. Using HPLC/ESI-MS-MS analysis, PPE was demonstrated to contain coumaroyl and caffeoyl derivatives among polyphenols. PPE at 0.5 or 1% was supplied to diabetic mice for 12 weeks. The PPE intake at two doses significantly improved glycaemic control. These treatments reserved the glutathione (GSH) level, and decreased the thiobarbituric acid reactive substances (TBARS) level, reactive oxygen species (ROS), interleukin (IL)-6, tumour necrosis factor (TNF)-alpha, fructose, and glycation intermediates and precursors of advanced glycation end products (AGEs), such as methylglyoxal (MG) and N-(carboxymethyl)lysine (CML), in the sciatic nerves of diabetic mice. In a histological study of sciatic nerves, PPE had the effects in improving the nerves of diabetic mice, showing disorganization of the fascicle with numerous small myelinated fibers. The PPE intake at two doses retained the activity, and the protein and mRNA levels of glutathione peroxidase (GPX), and decreased protein expressions of aldose reductase (AR) and the receptor for the advanced glycation end product (RAGE) in sciatic nerves. These findings support that pepino polyphenolic extract could attenuate oxidative, inflammatory and glycative stress in diabetic peripheral nerves.
authors.affiliation: Department of Electric Engineering, I-Shou University, Kaohsiung City, Taiwan and Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan.
authors.forename: Chin-Tsu
authors.initials: CT
authors.lastname: Ma
authors.affiliation: Research Institute of Biotechnology, Hung Kuang University, Taichung City, Taiwan.
authors.forename: Charng-Cherng
authors.initials: CC
authors.lastname: Chyau
authors.affiliation: Department of Nutrition, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Cheng-Chin
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan.
authors.forename: Shyh-Ming
authors.initials: SM
authors.lastname: Kuo
authors.affiliation: Department of Electric Engineering, I-Shou University, Kaohsiung City, Taiwan.
authors.forename: Chin-Wen
authors.initials: CW
authors.lastname: Chuang
authors.affiliation: Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung City, Taiwan and Clinical Laboratory, Chung Shan Medical University Hospital, Taichung City, Taiwan. cjh0828@csmu.edu.tw.
authors.forename: Hui-Hsuan
authors.initials: HH
authors.lastname: Lin
authors.affiliation: Department of Nutrition, Chung Shan Medical University, Taichung City, Taiwan and Clinical Laboratory, Chung Shan Medical University Hospital, Taichung City, Taiwan. cjh0828@csmu.edu.tw.
authors.forename: Jing-Hsien
authors.initials: JH
authors.lastname: Chen
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D005419:Flavonoids / Q000032:analysis; D005707:Gallic Acid / Q000032:analysis; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000235:genetics / Q000378:metabolism; D062385:Hydroxybenzoates / Q000032:analysis; D007328:Insulin / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*; D011134:Polysaccharides / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism; D032322:Solanum / Q000737:chemistry*; D013311:Streptozocin; D013312:Stress, Physiological / Q000187:drug effects*; D053719:Tandem Mass Spectrometry; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D001786:Blood Glucose; D005419:Flavonoids; D017127:Glycation End Products, Advanced; D062385:Hydroxybenzoates; D007328:Insulin; D015850:Interleukin-6; D010936:Plant Extracts; D059808:Polyphenols; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin; D005707:Gallic Acid; D000449:Aldehyde Reductase; D005979:Glutathione Peroxidase; C017616:phenolic acid