title: Spontaneously Arising Canine Glioma as a Potential Model for Human Glioma.
abstract: Human gliomas are malignant brain tumours that carry a poor prognosis and are composed of a heterogeneous population of cells. There is a paucity of animal models available for study of these tumours and most have been created by genetic modification. Spontaneously arising canine gliomas may provide a model for the characterization of the human tumours. The present study shows that canine gliomas form a range of immunohistochemical patterns that are similar to those described for human gliomas. The in-vitro sphere assay was used to analyze the expansion and differentiation potential of glioma cells taken from the periphery and centre of canine tumours. Samples from the subventricular zone (SVZ) and contralateral parenchyma were used as positive and negative controls, respectively. The expansion potential for all of these samples was low and cells from only three cultures were expanded for six passages. These three cultures were derived from high-grade gliomas and the cells had been cryopreserved. Most of the cells obtained from the centre of the tumours formed spheres and were expanded, in contrast to samples taken from the periphery of the tumours. Spheres were also formed and expanded from two areas of apparently unaffected brain parenchyma. The neurogenic SVZ contralateral samples also contained progenitor proliferating cells, since all of them were expanded for three to five passages. Differentiation analysis showed that all cultured spheres were multipotential and able to differentiate towards both neurons and glial cells. Spontaneously arising canine gliomas might therefore constitute an animal model for further characterization of these tumours.
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience and Cell Therapy Programme, Faculty of Medicine, Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Herranz
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra, Spain; Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain. Electronic address: Francisco.Fernandez.Flores@uab.cat.
authors.forename: F
authors.initials: F
authors.lastname: Fernández
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience and Cell Therapy Programme, Faculty of Medicine, Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Martín-Ibáñez
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Blasco
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience and Cell Therapy Programme, Faculty of Medicine, Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Crespo
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: C
authors.initials: C
authors.lastname: De la Fuente
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Añor
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: R M
authors.initials: RM
authors.lastname: Rabanal
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience and Cell Therapy Programme, Faculty of Medicine, Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Canals
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra, Spain; Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pumarola
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D005910:Glioma / Q000473:pathology / Q000662:veterinary*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male
keywords: animal model; dog; glioma; neurosphere