title: Nocardioides flava sp. nov., isolated from rhizosphere of poppy plant, Republic of Korea.
abstract: Phylogenetic and taxonomic characterization was performed for bacterium, designated strain THG-DN5.4(T), isolated from the rhizosphere of poppy plant collected from Gyeryongsan, Republic of Korea. Strain THG-DN5.4(T) consists of Gram-stain-positive, aerobic, non-motile rods. The bacteria grow optimally at 18-30 °C, at pH 7.0 and in the presence of 0.5-1.0 % NaCl. Based on 16S rRNA gene sequence analysis, strain THG-DN5.4(T) was found to be most closely related to Nocardioides nitrophenolicus KCTC 047BP(T), followed by Nocardioides ginsengisoli KCTC 19135(T), Nocardioides kongjuensis KCTC 19054(T), Nocardioides simplex KACC 20620(T), Nocardioides aromaticivorans KACC 20613(T), Nocardioides daeguensis KCTC 19799(T) and Nocardioides caeni KCTC 19600(T). The DNA-DNA relatedness between strain THG-DN5.4(T) and closely related phylogenetic neighbors was below 45.0 %, and the DNA G+C content of strain THG-DN5.4(T) was 70.8 mol%. An isoprenoid quinone was identified as MK-8(H4). Strain THG-DN5.4(T) was characterized chemotaxonomically as having LL-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, some unidentified aminolipids and some unidentified polar lipids. iso-C16:0 and C18:1 ω9c were identified as the major fatty acids present in THG-DN5.4(T). On the basis of a polyphasic taxonomic study, strain THG-DN5.4(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides flava sp. nov. is proposed. The type strain is THG-DN5.4(T) (=KCTC 39606(T)=CCTCC AB 2015298(T)).
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Hina
authors.initials: H
authors.lastname: Singh
authors.affiliation: Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University Seoul Campus, #1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. acyin@khu.ac.kr.
authors.forename: Chang Shik
authors.initials: CS
authors.lastname: Yin
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry; D010207:Papaver / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid
keywords: 16S rRNA; Gram-stain-positive; LL-Diaminopimelic acid; MK-8(H4); Nocardioides flava