title: The Inhibitory Mechanisms Study of 5,6,4'-Trihydroxy-7,3'-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability.
abstract: The whole plant of Anisomeles ovata has been widely used in Taiwan for treating inflammation-related skin and liver diseases, however, the detailed pharmacology mechanisms have yet to be elucidated. In the present study, one of the major components, 5,6,4'-trihydroxy-7,3'-dimethoxyflavone (5-TDMF), was purified from a methanol extract of Anisomeles ovata. A pharmacological study of this compound suggests that 5-TDMF possesses potent free radical scavenging activity both in vitro and ex vivo. Furthermore, 5-TDMF reduces nitric oxide and pro-inflammatory cytokine production in LPC-treated RAW 264.7 cells through the attenuation of nitric oxide synthase and cyclooxygenase-2. Additional experiments suggest that of 5-TDMF interferes with nuclear factor-κB translocation and mitogen-activated protein kinase pathways. These results identify 5-TDMF as an anti-oxidant and anti-inflammatory compound, explain the pharmacologic function of Anisomeles ovata and suggest its great potential as a new anti-inflammatory remedy.
authors.affiliation: School of Pharmacy, China Medical University, Taichung 404, Taiwan. isaacwangsh@gmail.com.
authors.forename: Shih-Hao
authors.initials: SH
authors.lastname: Wang
authors.affiliation: Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan. tinna_ling@mail.cnu.edu.tw.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Liang
authors.affiliation: Department of Pharmacy, Tajen University, Pingtung 907, Taiwan. ultramarine@seed.net.tw.
authors.forename: Fong-Pin
authors.initials: FP
authors.lastname: Liang
authors.affiliation: Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan. hsiou221@yahoo.com.tw.
authors.forename: Hsiou-Yu
authors.initials: HY
authors.lastname: Ding
authors.affiliation: School of Pharmacy, China Medical University, Taichung 404, Taiwan. splin@mail.cmu.edu.tw.
authors.forename: Shiuan-Pey
authors.initials: SP
authors.lastname: Lin
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan. gjhuang@mail.cmu.edu.tw.
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
authors.affiliation: School of Pharmacy, China Medical University, Taichung 404, Taiwan. wclin@mail.cmu.edu.tw.
authors.forename: Wen-Chuan
authors.initials: WC
authors.lastname: Lin
authors.affiliation: Department of Pharmacy, Tajen University, Pingtung 907, Taiwan. paul.juang@gmail.com.
authors.forename: Shin-Hun
authors.initials: SH
authors.lastname: Juang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D019686:Lamiaceae / Q000737:chemistry; D008070:Lipopolysaccharides / Q000009:adverse effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D000067996:RAW 264.7 Cells
chemical_list: C555500:5,6,4'-trihydroxy-7,3'-dimethoxyflavone; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D047309:Flavones; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
keywords: 5,6,4′-trihydroxy-7,3′-dimethoxyflavone; Anisomeles ovata; anti-inflammatory; anti-oxidant; reactive oxygen species