title: Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice.
abstract: Dendritic cells (DC) have been investigated as a cell-based therapy for Type 1 Diabetes (T1D). BM-DC expandedex vivowith GM-CSF and IL-4 is typically cultured with fetal bovine serum (FBS). The effect of FBS on NOD BM-DC has not been extensively studied. In the present study we compare BM-DC generated in serum-free culture media (X-VIVO20; FBS-) with BM-DC generated in media containing 10% FBS (RPMI1640/10%FBS; FBS+). We show that FBS- BM-DC display a phenotype and cytokine-producing profile distinct from FBS+ BMDC. Additionally, compared to FBS+ BM-DC, we show evidence of an altered Thcell response induced by FBS- BM-DC. Finally, we demonstrate that only FBS- BM-DC prevent the onset of T1D and induce increased levels of CD4+Foxp3+regulatory T cells as well as a long-lasting β cell-specific T cell response. This study indicates that serum-free media generates a more tolerogenic BM-DC capable of preventing T1D in the NOD mice.
authors.affiliation: Department of Pathology, Immunology and Laboratory Medicine, University of Florida, USA.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Looney
authors.affiliation: Department of Pathology, Immunology and Laboratory Medicine, University of Florida, USA.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Chernatynskaya
authors.affiliation: Department of Pathology, Immunology and Laboratory Medicine, University of Florida, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Clare-Salzler
authors.affiliation: Department of Pathology, Immunology and Laboratory Medicine, University of Florida, USA.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Xia
keywords: Bone marrow-derived dendritic cells; Fetal bovine serum; NOD mouse; Serum-free media; Type 1 diabetes