title: Factors influencing the prevalence and infestation levels of Varroa destructor in honeybee colonies in two highland agro-ecological zones of Uganda.
abstract: Varroa mites are ecto-parasites of honeybees and are a threat to the beekeeping industry. We identified the haplotype of Varroa mites and evaluated potential factors that influence their prevalence and infestation levels in the eastern and western highland agro-ecological zones of Uganda. This was done by collecting samples of adult worker bees between December 2014 and September 2015 in two sampling moments. Samples of bees were screened for Varroa using the ethanol wash method and the mites were identified by molecular techniques. All DNA sequences obtained from sampled mite populations in the two zones were 100 % identical to the Korean Haplotype (AF106899). Mean mite prevalence in the apiaries was 40 and 53 % for the western and eastern zones, respectively, during the first sampling. Over the second sampling, mean mite prevalence increased considerably in the western (59 %) but not in the eastern (51 %) zone. Factors that were associated with Varroa mite infestation levels include altitude, nature of apiary slope and apiary management practices during the first sampling. Our results further showed that Varroa mites were spreading from lower to higher elevations. Feral colonies were also infested with Varroa mites at infestation levels not significantly different from those in managed colonies. Colony productivity and strength were not correlated to mite infestation levels. We recommend a long-term Varroa mite monitoring strategy in areas of varying landscape and land use factors for a clear understanding of possible changes in mite infestation levels among African honeybees for informed decision making.
authors.affiliation: Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, 9000, Ghent, Belgium. mchemurot@cns.mak.ac.ug.|Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda. mchemurot@cns.mak.ac.ug.
authors.forename: Moses
authors.identifier: http://orcid.org/0000-0002-5270-2755
authors.initials: M
authors.lastname: Chemurot
authors.affiliation: Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Akol
authors.affiliation: Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors.forename: Charles
authors.initials: C
authors.lastname: Masembe
authors.affiliation: Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, 9000, Ghent, Belgium.
authors.forename: Lina
authors.initials: L
authors.lastname: de Smet
authors.affiliation: Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, 9000, Ghent, Belgium.
authors.forename: Tine
authors.initials: T
authors.lastname: Descamps
authors.affiliation: Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, 9000, Ghent, Belgium.
authors.forename: Dirk C
authors.initials: DC
authors.lastname: de Graaf
mesh_terms: D000818:Animals; D056630:Beekeeping; D001516:Bees / Q000469:parasitology*; D015995:Prevalence; D014454:Uganda; D057077:Varroidae / Q000502:physiology*
keywords: Apis mellifera; Ecto-parasite infestation levels; Mite prevalence; Varroa destructor