title: Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet.
abstract: The objective of this study was to investigate the antioxidant and hepatoprotective effect of the chronic use of conventional (CGJ) or organic (OGJ) grape juice from the Bordeaux variety grape on oxidative stress and cytoarchitecture in the liver of rats supplemented with a high-fat diet (HFD) for three months. The results demonstrated that HFD induced an increase in thiobarbituric acid-reactive substances (TBARS), catalase (CAT) activity and 2',7'-dihydrodichlorofluorescein (DCFH) oxidation and a decrease in sulfhydryl content and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. HFD also induced hepatocellular degeneration and steatosis. These alterations were prevented by CGJ and OGJ, where OGJ was more effective. Therefore, it was concluded that HFD induced oxidative stress and liver damage and that the chronic use of grape juice was able to prevent these alterations.
authors.affiliation: Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, Brazil. iseldeb@ig.com.br.
authors.forename: Iselde
authors.initials: I
authors.lastname: Buchner
authors.affiliation: Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, Brazil. niarasm@yahoo.com.br.
authors.forename: Niara
authors.initials: N
authors.lastname: Medeiros
authors.affiliation: Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, Brazil. denidsl@yahoo.com.br.
authors.forename: Denise Dos Santos
authors.initials: Ddos S
authors.lastname: Lacerda
authors.affiliation: Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, Brazil. cabmnormann@hotmail.com.
authors.forename: Carlos Augusto B M
authors.initials: CA
authors.lastname: Normann
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, Brazil. tantangemelli@gmail.com.
authors.forename: Tanise
authors.initials: T
authors.lastname: Gemelli
authors.affiliation: Departamento de Ciências Morfológicas, Universidade Federal do Rio Grande do Sul, 90040-060 Porto Alegre, Brazil. paula.rigon@ufrgs.br.
authors.forename: Paula
authors.initials: P
authors.lastname: Rigon
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, Brazil. clovisdw@ufrgs.br.
authors.forename: Clovis Milton Duval
authors.initials: CM
authors.lastname: Wannmacher
authors.affiliation: Departamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brazil. pegas@cbiot.ufrgs.br.|Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, 95070-560 Porto Alegre, Brazil. pegas@cbiot.ufrgs.br.
authors.forename: João Antônio Pegas
authors.initials: JA
authors.lastname: Henriques
authors.affiliation: Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, Brazil. caroline.dani@metodistadosul.edu.br.
authors.forename: Caroline
authors.initials: C
authors.lastname: Dani
authors.affiliation: Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, Brazil. claudia.funchal@metodistadosul.edu.br.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Funchal
keywords: antioxidants; free radicals; grapes; high-fat diet; liver