title: A Synthetic Glycopeptide Vaccine for the Induction of a Monoclonal Antibody that Differentiates between Normal and Tumor Mammary Cells and Enables the Diagnosis of Human Pancreatic Cancer.
abstract: In studies within the realm of cancer immunotherapy, the synthesis of exactly specified tumor-associated glycopeptide antigens is shown to be a key strategy for obtaining a highly selective biological reagent, that is, a monoclonal antibody that completely differentiates between tumor and normal epithelial cells and specifically marks the tumor cells in pancreas tumors. Mucin MUC1, which is overexpressed in many prevalent cancers, was identified as a promising target for this strategy. Tumor-associated MUC1 differs significantly from that expressed by normal cells, in particular by altered glycosylation. Structurally defined tumor-associated MUC1 cannot be isolated from tumor cells. We synthesized MUC1-glycopeptide vaccines and analyzed their structure-activity relationships in immunizations; a monoclonal antibody that specifically distinguishes between human normal and tumor epithelial cells was thus generated.
authors.affiliation: Institut für Organische Chemie, Universität Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Palitzsch
authors.affiliation: Institut für Organische Chemie, Universität Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Nikola
authors.initials: N
authors.lastname: Gaidzik
authors.affiliation: Institut für Immunologie, Universitätsmedizin der Universität Mainz, Langenbeckstrasse 1, G. 708, 55101, Mainz, Germany.
authors.forename: Natascha
authors.initials: N
authors.lastname: Stergiou
authors.affiliation: Fakultät für Naturwissenschaften, Technische Hochschule Köln, Kaiser-Wilhelm-Allee, G. E39, 51373, Leverkusen, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Stahn
authors.affiliation: Institut für Organische Chemie, Universität Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hartmann
authors.affiliation: Institut für Immunologie, Universitätsmedizin der Universität Mainz, Langenbeckstrasse 1, G. 708, 55101, Mainz, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Gerlitzki
authors.affiliation: Fakultät für Naturwissenschaften, Technische Hochschule Köln, Kaiser-Wilhelm-Allee, G. E39, 51373, Leverkusen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Teusch
authors.affiliation: Pathologisches Institut Celle, Wittinger Strasse 14, 29223, Celle, Germany.
authors.forename: Peer
authors.initials: P
authors.lastname: Flemming
authors.affiliation: Institut für Immunologie, Universitätsmedizin der Universität Mainz, Langenbeckstrasse 1, G. 708, 55101, Mainz, Germany.
authors.forename: Edgar
authors.initials: E
authors.lastname: Schmitt
authors.affiliation: Institut für Organische Chemie, Universität Mainz, Duesbergweg 10-14, 55128, Mainz, Germany. hokunz@uni-mainz.de.
authors.forename: Horst
authors.initials: H
authors.lastname: Kunz
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001940:Breast / Q000166:cytology*; D001943:Breast Neoplasms / Q000473:pathology*; D019496:Cancer Vaccines / Q000008:administration & dosage*; D005260:Female; D006020:Glycopeptides / Q000276:immunology*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000175:diagnosis*
chemical_list: D000911:Antibodies, Monoclonal; D019496:Cancer Vaccines; D006020:Glycopeptides
keywords: MUC1; antitumor vaccines; glycopeptides; tumor cell recognition; tumor-selective monoclonal antibodies