title: Role of ACE and AGT gene polymorphisms in genetic susceptibility to diabetes mellitus type 2 in a Brazilian sample.
abstract: The aim of the current study was to investigate the association between the InDel polymorphism in the angiotensin I-converting enzyme gene (ACE) and the rs699 polymorphism in the angiotensinogen gene (AGT) and diabetes mellitus type 2 (DM2) in a sample population from Southern Brazil. A case-control study was conducted with 228 patients with DM2 and 183 controls without DM2. The ACE InDel polymorphism was genotyped by polymerase chain reaction (PCR) with specific primers, followed by electrophoresis on 1.5% agarose gel. The AGT rs699 polymorphism was genotyped using a real-time PCR assay. No significant association between the ACE InDel polymorphism and DM2 was detected (P = 0.97). However, regarding the AGT rs699 polymorphism, DM2 patients had a significantly higher frequency of the AG genotype and lower frequency of the GG genotype when compared to the controls (P = 0.03). Our results suggest that there is an association between the AGT rs699 polymorphism and DM2 in a Brazilian sample.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Wollinger
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brasil.
authors.forename: S M
authors.initials: SM
authors.lastname: Dal Bosco
authors.affiliation: Programa de Pós-Graduação em Ambiente e Desenvolvimento, Centro Universitário UNIVATES, Lajeado, RS, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Rempe
authors.affiliation: Programa de Pós-Graduação em Qualidade Ambiental, Universidade FEEVALE, Novo Hamburgo, RS, Brasil.
authors.forename: S E M
authors.initials: SE
authors.lastname: Almeida
authors.affiliation: Programa de Pós-Graduação em Qualidade Ambiental, Universidade FEEVALE, Novo Hamburgo, RS, Brasil.
authors.forename: D B
authors.initials: DB
authors.lastname: Berlese
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brasil.
authors.forename: R P
authors.initials: RP
authors.lastname: Castoldi
authors.affiliation: Serviço de Hemodinâmica, Hospital Bruno Born, Lajeado, RS, Brasil.
authors.forename: M E
authors.initials: ME
authors.lastname: Arndt
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Contini
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brasil.|Programa de Pós-Graduação em Biociências, Universidade Federal de Ciências da Saúde de Porto Alegre.
authors.forename: J P
authors.initials: JP
authors.lastname: Genro
mesh_terms: D000368:Aged; D000483:Alleles; D000808:Angiotensinogen / Q000235:genetics* / Q000378:metabolism; D001938:Brazil; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D054643:INDEL Mutation; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D012084:Renin-Angiotensin System / Q000235:genetics; D012307:Risk Factors
chemical_list: C534931:AGT protein, human; D000808:Angiotensinogen; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A