title: Mesenchymal Stem Cells and Mononuclear Cells From Cord Blood: Cotransplantation Provides a Better Effect in Treating Myocardial Infarction.
abstract: The aim of this study was to evaluate the effect of cotransplanting mononuclear cells from cord blood (CB-MNCs) and mesenchymal stem cells (MSCs) as treatment for myocardial infarction (MI). Transplanting CD34+ cells or MSCs separately has been shown effective in treating MI, but the effect of cotransplanting CB-MNCs and MSCs is not clear. In this study, MSCs were separated by their adherence to the tissue culture. The morphology, immunophenotype, and multilineage potential of MSCs were analyzed. CB-MNCs were separated in lymphocyte separation medium 1.077. CD34+ cell count and viability were analyzed by flow cytometry. Infarcted male Sprague-Dawley rats in a specific-pathogen-free grade were divided into four treatment groups randomly: group I, saline; group II, CB-MNCs; group III, MSCs; and group IV, CB-MNCs plus MSCs. The saline, and CB-MNCs and/or MSCs were injected intramyocardially in infarcted rats. Their cardiac function was evaluated by echocardiography. The myocardial capillary density was analyzed by immunohistochemistry. Both cell types induced an improvement in the left ventricular cardiac function and increased tissue cell proliferation in myocardial tissue and neoangiogenesis. However, CB-MNCs plus MSCs were more effective in reducing the infarct size and preventing ventricular remodeling. Scar tissue was reduced significantly in the CB-MNCs plus MSCs group. MSCs facilitate engraftment of CD34+ cells and immunomodulation after allogeneic CD34+ cell transplantation. Cotransplanting MSCs and CB-MNCs might be more effective than transplanting MSCs or CB-MNCs separately for treating MI. This study contributes knowledge toward effective treatment strategies for MI.
authors.affiliation: Department of Cardiology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Gecai
authors.initials: G
authors.lastname: Chen
authors.affiliation: Jiangsu Province Stem Cell Bank, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Aihuan
authors.initials: A
authors.lastname: Yue
authors.affiliation: Department of Pathology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Cardiology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Zhongbao
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Department of Cardiology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Cardiology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Ruzhu
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Taizhou People Hospital, Taizhou, Jiangsu Province, People's Republic of China yueaihuan@beike.cc.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D036101:Cord Blood Stem Cell Transplantation; D004452:Echocardiography; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left
keywords: Cord blood mononuclear cells; Cotransplantation; Mesenchymal stem cells; Myocardial infarction