title: Ultrastructure and phylogeny of Kudoa barracudai sp. nov. infecting Sphyraena putnamae in the Red Sea.
abstract: Kudoa barracudai n. sp. has been found infecting the muscles of Sphyraena putnamae from Red Sea, Saudi Arabia. The prevalence of the infection was 10 % (5/50). Its ovoidal plasmodia encapsulated with fibrous connective tissue and measuring 700 × 510 μm. Local myolysis was observed around the plasmodia without inflammatory reaction. Mature spores were quadrate in the apical view with rounded edges and subspherical to ovoid in the side view and measured 5 μm (4.5-5.5) in width and 5.5 μm (5-6) in thickness. Polar capsules were pyriform, equal in size, and measured 2.5 μm (2-3) in length and 1.5 μm (1-2) in width with two filament coils. Ultrastructural analysis showed a demarcated border between the parasite cellular mass and host cellular components that represented the cyst wall, and cysts were filled with mostly mature spores. Phylogenetic analysis of the small subunit ribosomal RNA (SSU rRNA) using the maximum likelihood and Bayesian inference approaches showed the new species clustered in a robust clade with Kudoa amamiensis, Kudoa kenti, and Kudoa quraishii. The SSU rRNA gene of K. barracudai was most similar to K. amamiensis (98.7 %), followed by K. kenti (97.4 %) and K. quraishii (96.6 %). This combination of morphological data and molecular analysis served to identify this parasite as a new species of Kudoa, which we have named K. barracudai n. sp.
authors.affiliation: Zoology Department, College of Science, King Saud University, Saudi Arabia, P.O. Box 2455, Riyadh, 11451, Saudi Arabia. azema1@yahoo.com.|Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. azema1@yahoo.com.
authors.forename: Abdel-Azeem S
authors.initials: AA
authors.lastname: Abdel-Baki
authors.affiliation: Zoology Department, College of Science, King Saud University, Saudi Arabia, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Quraishy
authors.affiliation: Zoology Department, College of Science, King Saud University, Saudi Arabia, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
authors.forename: Suliman Y
authors.initials: SY
authors.lastname: Al Omar
authors.affiliation: Zoology Department, College of Science, King Saud University, Saudi Arabia, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.|Unité de Recherche de Biologie intégrative et Ecologie évolutive et Fonctionnelle des Milieux Aquatiques, Département de Biologie, Faculté des Sciences de Tunis, Université De Tunis El Manar, Tunis, Tunisia.
authors.forename: Lamjed
authors.initials: L
authors.lastname: Mansour
mesh_terms: D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D019156:Indian Ocean; D009132:Muscles / Q000469:parasitology*; D055764:Myxozoa / Q000145:classification / Q000235:genetics / Q000648:ultrastructure; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D010473:Perciformes / Q000469:parasitology*; D010802:Phylogeny; D054679:Ribosome Subunits, Small / Q000235:genetics; D012529:Saudi Arabia; D017422:Sequence Analysis, DNA; D013170:Spores / Q000648:ultrastructure
chemical_list: D004275:DNA, Ribosomal
keywords: Muscle; Myxozoa; New species; Parasite; SSU rRNA gene