title: Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste.
abstract: Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-l-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9mg of Protein G per g of the cryogel.
authors.affiliation: Hacettepe University, Department of Chemistry, Ankara, Turkey; Department of Biotechnology, Lund University, Sweden.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Asliyuce
authors.affiliation: Department of Biotechnology, Lund University, Sweden; Indienz AB, Annebergs Gård, Billeberga, Sweden.
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
authors.affiliation: Department of Biotechnology, Lund University, Sweden; Indienz AB, Annebergs Gård, Billeberga, Sweden. Electronic address: gashaw.mamo@biotek.lu.se.
authors.forename: Gashaw
authors.initials: G
authors.lastname: Mamo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002457:Cell Extracts / Q000737:chemistry; D059985:Cryogels / Q000737:chemistry*; D016014:Linear Models; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D002457:Cell Extracts; D059985:Cryogels; C034284:IgG Fc-binding protein, Streptococcus; D011108:Polymers; D012965:Sodium Chloride
keywords: Antibodies; Macroporous; Molecular printing; Recombinant protein G