title: Molecular surveillance of Theileria equi and Anaplasma phagocytophilum infections in horses from Ukraine, Poland and Slovakia.
abstract: A survey was undertaken to assess the prevalence of Theileria equi and Anaplasma phagocytophilum in some regions of Ukraine, Poland and Slovakia. Using a specific PCR assays, blood samples from 215 horses were tested. The prevalence of T. equi and A. phagocytophilum infection was 13.95% and 1.4%, respectively. BLAST analysis showed the isolates closest to the T. equi 18S rRNA and A. phagocytophilum msp4 gene sequences in GenBank with a similarity of ≥99%. No significant association was found between the T. equi PCR positivity and the age or sex of the horses. There was a significant association between the origin of horses and T. equi-PCR positivity. No significant association was found between the A. phagocytophilum-PCR positivity and the age, sex or origin.
authors.affiliation: I.I. Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine, vul. Bogdana Khmelnitskogo 15, 01601 Kyiv, Ukraine.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Slivinska
authors.affiliation: Institute of Parasitology of Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
authors.forename: Bronislava
authors.initials: B
authors.lastname: Víchová
authors.affiliation: W. Stefański Institute of Parasitology of Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Werszko
authors.affiliation: W. Stefański Institute of Parasitology of Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szewczyk
authors.affiliation: Veterinary Private Practice, Mickiewicza 41, Pisz, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Wróblewski
authors.affiliation: Institute of Parasitology of Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
authors.forename: Branislav
authors.initials: B
authors.lastname: Peťko
authors.affiliation: Institute of Parasitology of Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Ragač
authors.affiliation: Wroclaw University of Environmental and Life Sciences, Norwida 25/27, 50-375 Wroclaw, Poland.
authors.forename: Vitaliy
authors.initials: V
authors.lastname: Demeshkant
authors.affiliation: W. Stefański Institute of Parasitology of Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland. Electronic address: grzgrz@twarda.pan.pl.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Karbowiak
mesh_terms: D041081:Anaplasma phagocytophilum; D000818:Animals; D016873:Ehrlichiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology*; D006736:Horses; D008297:Male; D011044:Poland / Q000453:epidemiology; D011159:Population Surveillance; D018154:Slovakia / Q000453:epidemiology; D016794:Theileria / Q000145:classification*; D013801:Theileriasis / Q000453:epidemiology / Q000469:parasitology*; D014455:Ukraine / Q000453:epidemiology
keywords: Anaplasma phagocytophilum; Horse; Poland; Slovakia; Theileria equi; Ukraine