title: Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes.
abstract: Hepatocytes differentiated from induced pluripotent stem cells and adult stem cells could be utilized as a tool for the study of liver diseases, screening for drug metabolism and hepatotoxicity. Thus further investigation of the method to efficiently generate hepatocytes is in great need. Bone Mesenchymal Stem Cells (BMSCs) were collected from rat femurs and tibias. FOXA2 and HNF1α genes were constructed into a lentiviral vector and introduced into BMSCs by a lentivirus-mediated overexpression system. Three weeks after the induction, the expressions of FOXA2 and HNF1α, and liver specific genes were analyzed, and hepatocyte-function related assays were performed. Overexpression of both FOXA2 and HNF1α induced the BMSCs to differentiate into hepatocyte-like cells (HLCs). Hepatocyte-specific gene and protein were detected by RT-PCR, Western Blot and Immunofluorescence. These HLCs also exerted some typical hepatocyte functions such as glycogen storage, indocyanine green absorption and lipid accumulation. The combination of FOXA2 and HNF1α can effectively induce BMSCs to differentiate into HLCs. This is a novel and efficient method to prepare HLCs within a short timeline.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Chang
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Niu
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Dai
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Geng
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007, Henan Province, China. cellkeylab@126.com.|State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, 453007, Henan Province, China. cellkeylab@126.com.|Henan Engineering Laboratory for Bioengineering and Drug Development, Xinxiang, China. cellkeylab@126.com.
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
keywords: Bone marrow mesenchymal stem cells; FOXA2; HNF1α; Hepatocyte-like cells; Lentivirus