title: Recombinant production and purification of short hydrophobic Elastin-like polypeptides with low transition temperatures.
abstract: Elastin-like polypeptides (ELPs) are biodegradable polymers with interesting physico-chemical properties for biomedical and biotechnological applications. We report herein the recombinant expression of three hydrophobic ELPs (VPGIG)n with variable lengths (n = 20, 40, 60) and sub-ambient transition temperatures. These ELPs were purified from the cytoplasmic soluble fraction of Escherichia coli by inverse transition cycling, and their exact molecular weight was confirmed by various mass spectrometry techniques. Transition temperatures of ELP20, ELP40, and ELP60 were measured at 18.6 °C, 12.4 °C and 11.7 °C, respectively.
authors.affiliation: University of Bordeaux, LCPO, UMR 5629, F-33600, Pessac, France; CNRS, LCPO, UMR 5629, F-33600, Pessac, France; Bordeaux INP, LCPO, UMR 5629, F-33600, Pessac, France; Institut Européen de Chimie et Biologie, F-33600, Pessac, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Bataille
authors.affiliation: University of Bordeaux, BPRVS, EA4135, F-33000, Bordeaux, France; Bordeaux INP, BPRVS, EA4135, F-33000, Bordeaux, France.
authors.forename: Wilfrid
authors.initials: W
authors.lastname: Dieryck
authors.affiliation: University of Bordeaux, BPRVS, EA4135, F-33000, Bordeaux, France; Bordeaux INP, BPRVS, EA4135, F-33000, Bordeaux, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Hocquellet
authors.affiliation: University of Bordeaux, BPRVS, EA4135, F-33000, Bordeaux, France; Bordeaux INP, BPRVS, EA4135, F-33000, Bordeaux, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Cabanne
authors.affiliation: University of Bordeaux, CBMN, UMR 5248, F-33600, Pessac, France; CNRS, CBMN, UMR5248, F-33600, Pessac, France; Bordeaux INP, CBMN, UMR5248, F-33600, Pessac, France.
authors.forename: Katell
authors.initials: K
authors.lastname: Bathany
authors.affiliation: University of Bordeaux, LCPO, UMR 5629, F-33600, Pessac, France; CNRS, LCPO, UMR 5629, F-33600, Pessac, France; Bordeaux INP, LCPO, UMR 5629, F-33600, Pessac, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lecommandoux
authors.affiliation: University of Bordeaux, BPRVS, EA4135, F-33000, Bordeaux, France; Bordeaux INP, BPRVS, EA4135, F-33000, Bordeaux, France. Electronic address: bertrand.garbay@ipb.fr.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Garbay
authors.affiliation: University of Bordeaux, LCPO, UMR 5629, F-33600, Pessac, France; CNRS, LCPO, UMR 5629, F-33600, Pessac, France; Bordeaux INP, LCPO, UMR 5629, F-33600, Pessac, France; Institut Européen de Chimie et Biologie, F-33600, Pessac, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Garanger
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D004549:Elastin / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D044366:Transition Temperature
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D004549:Elastin
keywords: Elastin-like polypeptides; Mass spectrometry; Precision polymers; Recombinant expression; Transition temperature