title: Immunoprotective Effect of Probiotic Dahi Containing Lactobacillus acidophilus and Bifidobacterium bifidum on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice.
abstract: In the present study, probiotic Dahi (LaBb Dahi) containing Lactobacillus acidophilus LaVK2 and Bifidobacterium bifidum BbVK3 was selected as a probiotic therapy to investigate its protective effect on dextran sodium sulfate (DSS)-induced ulcerative colitis model in mice that mimics the picture in human. LaBb Dahi was prepared by co-culturing Dahi bacteria (Lactococcus lactis ssp. cremoris NCDC-86 and Lactococcus lactis ssp. lactis biovar diacetylactis NCDC-60) along with selected strain of L. acidophilus LaVK2 and B. bifidum BbVK3 in buffalo milk (3% fat). Four groups of swiss albino male mice (12 each) were fed buffalo milk (3% fat), buffalo milk (3% fat) plus DSS, Dahi plus DSS, and LaBb Dahi plus DSS, respectively, for 17 days with basal diet. The myeloperoxidase (MPO) activity, levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interferon (IFN-γ) were assessed as inflammatory markers, and the histopathological picture of the colon of mice was studied. DSS-induced colitis appeared to induce significant increase in MPO activity, levels of TNF-α, IL-6 and IFN-γ. Feeding with LaBb Dahi offered significant reduction in MPO activity, levels of TNF-α, IL-6 and IFN-γ when compared to either buffalo milk group or group III (Dahi). The present study suggests that LaBb probiotic Dahi can be used to combat DSS-induced biochemical and histological changes and to achieve more effective treatment for ulcerative colitis.
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: Sagar R
authors.initials: SR
authors.lastname: Jadhav
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, Haryana, 132001, India. ukshandilya@gmail.com.
authors.forename: Umesh Kr
authors.initials: UK
authors.lastname: Shandilya
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, Haryana, 132001, India.
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Kansal
keywords: B. bifidum; Dextran sodium sulfate; L. acidophilus; LaBb Dahi; Myeloperoxidase; Ulcerative colitis