title: Histone H3K27 Demethylase JMJD3 in Cooperation with NF-κB Regulates Keratinocyte Wound Healing.
abstract: Histone H3K27me3 demethylase JMJD3 has been shown to be involved in keratinocyte differentiation and wound healing. However, the exact molecular mechanism underlying JMJD3-mediated keratinocyte wound healing has not been fully elucidated. In this study, we report on the biological function of JMJD3 in keratinocyte wound healing using in vitro cell and in vivo animal models. Our results indicate that JMJD3 up-regulation and NF-κB activation occur in the region of the wound edge during keratinocyte wound healing. We also found that JMJD3 interacts with NF-κB, resulting in increased expression of the inflammatory, matrix metalloproteinase, and growth factor genes via demethylation of H3K27me3 at the gene promoters. Consistently, inactivation of JMJD3 or NF-κB resulted in aberrant keratinocyte wound healing. Our study suggests that regulation of JMJD3 may provide a new therapeutic intervention for treating the chronic skin wound.
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea.
authors.forename: Jungtae
authors.initials: J
authors.lastname: Na
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea.
authors.forename: Kwanghyun
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea.
authors.forename: Wonho
authors.initials: W
authors.lastname: Na
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea.
authors.forename: Jee-Yoon
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Molecular Biology and Age-Related and Brain Diseases Research Center, School of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Tae Young
authors.initials: TY
authors.lastname: Yune
authors.affiliation: Amore Pacific Corporation/R&D center, Yongin-Si Gyeonggi-do, Korea.
authors.forename: Hae Kwang
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea; Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR.
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Jung
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Kim
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea. Electronic address: bgju@sogang.ac.kr.
authors.forename: Bong-Gun
authors.initials: BG
authors.lastname: Ju
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D006657:Histones / Q000737:chemistry; D006801:Humans; D007249:Inflammation; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000201:enzymology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism; D014945:Wound Healing
chemical_list: D006657:Histones; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D034741:RNA, Small Interfering; C073701:Nfkb1 protein, mouse; D056484:Jumonji Domain-Containing Histone Demethylases; C524220:KDM6B protein, human; C523761:Kdm6b protein, mouse