title: Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease.
abstract: Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was confirmed to be the Hami melon Cu/Zn-SOD gene sequence. Real-time fluorescence quantitative expression analysis indicated that there were differences in the expression of SOD mRNA expression before and after infection by blue mold. mRNA expression was maximal 24-h after infection, indicating that the product of the SOD gene plays an important role in the rotting and degeneration of Hami melons as a consequence of bacterial infection during the preservation period.
authors.affiliation: School of Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.|Food College of Shihezi University, Shihezi, Xinjiang, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Shan
authors.affiliation: Food College of Shihezi University, Shihezi, Xinjiang, China.
authors.forename: F X
authors.initials: FX
authors.lastname: Tang
authors.affiliation: School of Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.affiliation: Food College of Shihezi University, Shihezi, Xinjiang, China.
authors.forename: W R
authors.initials: WR
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018553:Cucumis sativus / Q000235:genetics* / Q000382:microbiology; D019662:Cucurbitaceae / Q000235:genetics* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D013482:Superoxide Dismutase