title: Proteomic Analysis of the Schistosoma mansoni Miracidium.
abstract: Despite extensive control efforts, schistosomiasis continues to be a major public health problem in developing nations in the tropics and sub-tropics. The miracidium, along with the cercaria, both of which are water-borne and free-living, are the only two stages in the life-cycle of Schistosoma mansoni which are involved in host invasion. Miracidia penetrate intermediate host snails and develop into sporocysts, which lead to cercariae that can infect humans. Infection of the snail host by the miracidium represents an ideal point at which to interrupt the parasite's life-cycle. This research focuses on an analysis of the miracidium proteome, including those proteins that are secreted. We have identified a repertoire of proteins in the S. mansoni miracidium at 2 hours post-hatch, including proteases, venom allergen-like proteins, receptors and HSP70, which might play roles in snail-parasite interplay. Proteins involved in energy production and conservation were prevalent, as were proteins predicted to be associated with defence. This study also provides a strong foundation for further understanding the roles that neurohormones play in host-seeking by schistosomes, with the potential for development of novel anthelmintics that interfere with its various life-cycle stages.
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.
authors.forename: Tianfang
authors.initials: T
authors.lastname: Wang
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.
authors.forename: Bronwyn A
authors.initials: BA
authors.lastname: Rotgans
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.
authors.forename: April
authors.initials: A
authors.lastname: Strong
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.
authors.forename: Di
authors.initials: D
authors.lastname: Liang
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.|School of Medical Science, Griffith Health Institute, Griffith University, Gold Coast, Queensland, 4222, Australia.
authors.forename: Guoying
authors.initials: G
authors.lastname: Ni
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Yanin
authors.initials: Y
authors.lastname: Limpanont
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Pongrama
authors.initials: P
authors.lastname: Ramasoota
authors.affiliation: QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
authors.affiliation: Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, 4558, Australia.
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000276:immunology* / Q000469:parasitology; D058487:Cercaria / Q000235:genetics* / Q000254:growth & development; D004199:Disease Vectors; D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D018503:Genome, Protozoan / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics / Q000276:immunology; D008018:Life Cycle Stages; D051379:Mice; D009479:Neuropeptides / Q000235:genetics; D033661:Oocysts / Q000254:growth & development; D020543:Proteome / Q000235:genetics; D040901:Proteomics; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology* / Q000469:parasitology
chemical_list: D009479:Neuropeptides; D020543:Proteome; D015800:Protozoan Proteins