title: Cycloartane and oleanane-type glycosides from Astragalus pennatulus.
abstract: Four new cycloartane and one new oleanane-type glycosides were isolated from Astragalus pennatulus along with five known cycloartane-type glycosides. The structures of the new compounds were established as 3-O-β-D-xylopyranosyl-6-O-β-D-glucopyranosyl-3β,6α,16β-trihydroxy-24-oxo-20(R),25-epoxycycloartane (1), 3-O-[β-D-glucuronopyranosyl-(1 → 2)-β-D-xylopyranosyl]-3β,16β,24α-trihydroxy-20(R),25-epoxycycloartane (2), 3-O-[β-D-glucuronopyranosyl-(1 → 2)-β-D-xylopyranosyl]-3β,16β,25-trihydroxy-20(R),24(S)-epoxycycloartane (3), 3,25-di-O-β-D-glucopyranosyl-6-O-β-D-xylopyranosyl-3β,6α,16β,25-tetrahydroxy-20(R),24(S)-epoxycycloartane (4), 29-O-α-L-rhamnopyranosyl-abrisapogenol B (5) by 1D and 2D-NMR experiments along with ESIMS and HRMS analyses. The aglycone of compound 1, 3β,6α,16β-trihydroxy-24-oxo-20(R),25-epoxycycloartane, is reported for the first time. The cytotoxic activity of the isolated compounds was evaluated against three cancer cell lines including A549 (human lung adenocarcinoma), A375 (human melanoma) and DeFew (human B lymphoma) cells. None of the tested compounds caused a significant reduction of the cell number.
authors.affiliation: Department of Chemistry, Faculty of Science, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Rabia
authors.initials: R
authors.lastname: Un
authors.affiliation: Department of Chemistry, Faculty of Science, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Horo
authors.affiliation: Department of Pharmacy, Salerno University, 84084 Fisciano, Salerno, Italy. Electronic address: mmasullo@unisa.it.
authors.forename: Milena
authors.initials: M
authors.lastname: Masullo
authors.affiliation: Department of Pharmacy, Salerno University, 84084 Fisciano, Salerno, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Falco
authors.affiliation: Department of Biology, Faculty of Science, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Serdar G
authors.initials: SG
authors.lastname: Senol
authors.affiliation: Department of Pharmacy, Salerno University, 84084 Fisciano, Salerno, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.affiliation: Department of Chemistry, Faculty of Science, Ege University, 35100 Bornova, İzmir, Turkey. Electronic address: ozgen.alankus.caliska@ege.edu.tr.
authors.forename: Özgen
authors.initials: Ö
authors.lastname: Alankuş-Çalıskan
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D045744:Cell Line, Tumor; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D006027:Glycosides; D014315:Triterpenes; C413246:oleanane; C085352:cycloartane; D009828:Oleanolic Acid
keywords: Astragalus pennatulus; Cycloartane saponins; Cyclocephalogenin; Fabaceae