title: Mayaro virus and dengue virus 1 and 4 natural infection in culicids from Cuiabá, state of Mato Grosso, Brazil.
abstract: This study aimed to verify the diversity of Culicidae species and their frequency of infection with flaviviruses and alphaviruses in Cuiabá, state of Mato Grosso, Brazil. Mosquitoes were captured with Nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). Female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (RT-PCR) for 11 flavivirus and five alphavirus. Positive pools were tested by single RT-PCR followed by nucleotide sequencing, by RT-PCR for E1 gene [Mayaro virus (MAYV)] and by inoculation in Vero cells (MAYV) or C6/36 cells (flaviviruses). One/171 Aedes aegypti was positive for dengue virus (DENV)-1, 12/403 Culex quinquefasciatus, and four/171Ae. aegypti for MAYV, which was isolated from two pools containing two nonengorged females of Ae. aegypti and two of Cx. quinquefasciatus. DENV-4 was detected in 58/171 pools of Ae. aegytpi, 105/403 Cx. quinquefasciatus, two/five Psorophora sp., two/11 Psorophora varipes/Psorophora albigenu, one/one Sabethes chloropterus, two/five Culex bidens/Culex interfor, and one/one Aedes sp. DENV-4 was isolated from two pools containing three and 16 nonengorged Cx. quinquefasciatus females. Phylogenetic analysis revealed MAYV belongs to genotype L, clustering with human samples of the virus previously identified in the city. Cuiabá has biodiversity and ecosystem favourable for vector proliferation, representing a risk for arbovirus outbreaks.
authors.affiliation: Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Otacília Pereira
authors.initials: OP
authors.lastname: Serra
authors.affiliation: Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Belgath Fernandes
authors.initials: BF
authors.lastname: Cardoso
authors.affiliation: Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Ana Lúcia Maria
authors.initials: AL
authors.lastname: Ribeiro
authors.affiliation: Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Fábio Alexandre Leal dos
authors.initials: FA
authors.lastname: Santos
authors.affiliation: Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Renata Dezengrini
authors.initials: RD
authors.lastname: Slhessarenko
mesh_terms: D000524:Alphavirus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001938:Brazil; D009033:Culicidae / Q000145:classification / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000821:virology*; D060885:Multiplex Polymerase Chain Reaction; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons