title: Application of Allogeneic Fibroblast Cultured on Acellular Amniotic Membrane for Full-thickness Wound Healing in Rats.
abstract: OBJECTIVE
  Utilization of the autologous and allogeneic skin substitutes seems to be a promising treatment option. In this study, the authors used amniotic membrane covered with cultured allogenic fibroblast as a skin substitute in the treatment of acute wounds.
  
  
  METHODS
  Full-thickness wounds were created on rats' dorsum regions and treated with cultured allogenic fibroblast on an acellular amniotic membrane (AAM+F), an acellular amniotic membrane (AAM) alone, an allogenic fibroblast suspension (AFS), or normal saline as a control (C). Specimen biopsies were obtained 7 days after wounding. Quantitative wound healing parameters including the epidermal thickness, the mean number of keratinocytes, fibroblasts, and lymphocytes were assessed.
  
  
  RESULTS
  All transplanted wounds exhibited significantly further contraction compared with the nontransplanted wounds. Wounds transplanted with AAM+F and AAM showed a significant increase in epidermal thickness compared to nontransplanted wounds. Wounds transplanted with AAM+F or AAM showed improved epidermal healing compared to nongrafted wounds. Furthermore, granulation of tissue formation in the AAM+F group was more organized when compared to AFS and the normal saline groups.
  
  
  CONCLUSIONS
  Quantitative assessment of the full-thickness wounds showed transplantation of AAM+F and AAM better improve wound healing parameters when compared to treatment with AFS and the normal saline groups.
authors.affiliation: Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Mahmoudi Rad
authors.affiliation: Anatomy Department, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Talebpour Amiri
authors.affiliation: Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Amol, Iran.
authors.forename: Mehri
authors.initials: M
authors.lastname: Mirhoseini
authors.affiliation: Pathology Department, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghasemi
authors.affiliation: Pathology Department, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Ira.
authors.forename: Mansooreh
authors.initials: M
authors.lastname: Mirzaei
authors.affiliation: Immunology Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Nariman
authors.initials: N
authors.lastname: Mosaffa
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism* / Q000637:transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries / Q000473:pathology*; D017768:Skin, Artificial; D014182:Transplantation, Autologous; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology* / Q000601:surgery*