title: KLF5 promotes cell migration by up-regulating FYN in bladder cancer cells.
abstract: Krüppel-like factor 5 (KLF5) promotes cell proliferation of bladder cancer. However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF5 knockdown. Furthermore, the KLF5/FYN/p-FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF5/FYN/p-FAK axis as a potential therapeutic target in bladder cancer.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Du
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.|Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.|Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Jinhai
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.|Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Xinyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.|Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Dalin
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.|Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D009363:Neoplasm Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D051076:Proto-Oncogene Proteins c-fyn / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D011554:Pseudopodia / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C492214:KLF5 protein, human; D051741:Kruppel-Like Transcription Factors; D008246:Lysophospholipids; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C494957:FYN protein, human; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D051076:Proto-Oncogene Proteins c-fyn; C032881:lysophosphatidic acid
keywords: FAK; FYN; KLF5; bladder cancer; cell migration