title: A strip array for spoligotyping of Mycobacterium tuberculosis complex isolates.
abstract: A novel strip array was developed for a nine-spacer spoligotyping scheme of Mycobacterium tuberculosis complex (MTBC). The new method was evaluated using 211 MTBC isolates and the results were fully concordant with the traditional spoligotyping approach. The strip array proved to be rapid and convenient for spoligotyping of MTBC.
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China; Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian 361102, China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China; Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian 361102, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Tuberculosis Reference Laboratory, Henan Center for Disease Control and Prevention, Zhengzhou, Henan 450016, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Division of Tuberculosis Control and Prevention, Xiamen Center for Disease Control and Prevention, Xiamen, Fujian 361021, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China; Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian 361102, China. Electronic address: xuye@xmu.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China; Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian 361102, China. Electronic address: qgli@xmu.edu.cn.
authors.forename: Qingge
authors.initials: Q
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D001483:Base Sequence; D003101:Collodion; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015345:Oligonucleotide Probes / Q000235:genetics; D009841:Oligonucleotides / Q000032:analysis; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011934:Reagent Strips; D013045:Species Specificity; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D011934:Reagent Strips; D003101:Collodion
keywords: Genotyping; Lateral flow hybridization; Mycobacterium tuberculosis complex