title: Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP.
abstract: The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of the rpsL gene in X. oryzae pv. oryzicola and X. oryzae pv. oryzae. The 304-bp PCR product from the rpsL gene was digested by MboII to form two fragments (201 and 103 bp) if there was a mutation at codon 43, or three fragments (146, 103, and 55 bp) if there was no mutation. Compared with the results from nucleotide sequencing, the PCR-RFLP method was accurate in detecting the point mutation at codon 43 of the rpsL gene in streptomycin-resistant strains of X. oryzae pv. oryzicola and X. oryzae pv. oryzae. These results indicate that the PCR-RFLP is a simple, rapid and reliable method for detecting the point mutation at codon 43 of the rpsL gene.
authors.affiliation: Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.|College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Zhou
authors.affiliation: Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
authors.forename: A F
authors.initials: AF
authors.lastname: Zhang
authors.affiliation: College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.|College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: M G
authors.initials: MG
authors.lastname: Zhou
authors.affiliation: Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
authors.forename: T C
authors.initials: TC
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D024881:Drug Resistance, Bacterial; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014974:Xanthomonas / Q000187:drug effects / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003062:Codon; D012269:Ribosomal Proteins