title: Structure of bacterial respiratory complex I.
abstract: Complex I (NADH:ubiquinone oxidoreductase) plays a central role in cellular energy production, coupling electron transfer between NADH and quinone to proton translocation. It is the largest protein assembly of respiratory chains and one of the most elaborate redox membrane proteins known. Bacterial enzyme is about half the size of mitochondrial and thus provides its important "minimal" model. Dysfunction of mitochondrial complex I is implicated in many human neurodegenerative diseases. The L-shaped complex consists of a hydrophilic arm, where electron transfer occurs, and a membrane arm, where proton translocation takes place. We have solved the crystal structures of the hydrophilic domain of complex I from Thermus thermophilus, the membrane domain from Escherichia coli and recently of the intact, entire complex I from T. thermophilus (536 kDa, 16 subunits, 9 iron-sulphur clusters, 64 transmembrane helices). The 95Å long electron transfer pathway through the enzyme proceeds from the primary electron acceptor flavin mononucleotide through seven conserved Fe-S clusters to the unusual elongated quinone-binding site at the interface with the membrane domain. Four putative proton translocation channels are found in the membrane domain, all linked by the central flexible axis containing charged residues. The redox energy of electron transfer is coupled to proton translocation by the as yet undefined mechanism proposed to involve long-range conformational changes. This article is part of a Special Issue entitled Respiratory complex I, edited by Volker Zickermann and Ulrich Brandt.
authors.affiliation: European Bioinformatics Institute, Cambridge CB10 1SD, UK.
authors.forename: John M
authors.initials: JM
authors.lastname: Berrisford
authors.affiliation: Memorial Sloan-Kettering Cancer Center, 430 E 67th Street, NY 10065, USA.
authors.forename: Rozbeh
authors.initials: R
authors.lastname: Baradaran
authors.affiliation: Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria. Electronic address: sazanov@ist.ac.at.
authors.forename: Leonid A
authors.initials: LA
authors.lastname: Sazanov
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D017494:Proton Pumps / Q000737:chemistry / Q000648:ultrastructure; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D017494:Proton Pumps; D042967:Electron Transport Complex I
keywords: Complex I; Electron transfer; Membrane protein; Proton translocation; Respiratory chain; X-ray crystallography