title: Simultaneous determination of glucose, fructose, sucrose and sorbitol in the leaf and fruit peel of different apple cultivars by the HPLC-RI optimized method.
abstract: A high performance liquid chromatography method with refractive index detection (HPLC-RI), for simultaneous determination of glucose, fructose, sucrose and sorbitol in leaf and/or apple peel samples from nine apple (Malus domestica Borkh.) cultivars and rootstocks, originating from a germplasm collection, has been developed and validated. Box-Behnken design of response surface methodology was applied for the method optimization. The Carbosep Coregel 87H3 column was used under the optimum conditions predicted: mobile phase of H2SO4 0.005 mol L(-1) solution, flow rate of 0.3 mL min(-1) and column temperature of 35°C. The method was validated for linearity (R(2)>0.99), limits of detection (2.67-4.83 μg mL(-1)) and quantification (8.9-16.1 μg mL(-1)), precision (%RSD<5.05) and recovery (93.94-103.06%) and satisfactory results obtained. The sugars content varied across micropropagated plants in vitro, plants regenerated after cryostorage, growing trees in vivo, and fruit peel.
authors.affiliation: Babeş-Bolyai University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Street, 400294 Cluj-Napoca, Romania. Electronic address: filip_miuta@yahoo.com.
authors.forename: Miuţa
authors.initials: M
authors.lastname: Filip
authors.affiliation: Babeş-Bolyai University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Street, 400294 Cluj-Napoca, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Vlassa
authors.affiliation: Babeş-Bolyai University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Street, 400294 Cluj-Napoca, Romania.
authors.forename: Virginia
authors.initials: V
authors.lastname: Coman
authors.affiliation: Institute of Biological Research Cluj-Napoca, Branch of National Institute of Research and Development for Biological Sciences, 48 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Adela
authors.initials: A
authors.lastname: Halmagyi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D005632:Fructose / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000032:analysis / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013012:Sorbitol / Q000032:analysis / Q000737:chemistry*; D013395:Sucrose / Q000032:analysis / Q000737:chemistry*
chemical_list: D005503:Food Additives; D005632:Fructose; D013012:Sorbitol; D013395:Sucrose; D005947:Glucose
keywords: Apple leaf and peel; Box–Behnken design; Fructose; Glucose; HPLC–RI; RSM optimization method; Sorbitol; Sucrose