title: Streptomyces arcticussp. nov., isolated from frozen soil.
abstract: Actinomycete strain ZLN234Twas isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genusStreptomyces. The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related toStreptomyces olivochromogenesATCC 3336T(98.63 % similarity) andStreptomyces aureusB7319T(98.56 %). Phylogenetic analysis based on thegyrB gene also showed clearly that strain ZLN234Twas different from all recognizedStreptomycesspecies. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine andmeso-diaminopimelic acid with a minor amount of ll-diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H10), MK-9(H8) and MK-9(H4). The major cellular fatty acids comprised anteiso-C15 : 0, iso-C16 : 0and C16 : 0with a small amount of iso-C16 : 1ω7 and anteiso-C14 : 0. The DNA G+C content of strain ZLN234Twas 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234T( = CCTCC AA 2015005T= DSM 100713T) is the type strain of a novel species of the genusStreptomyces, for which the nameStreptomyces arcticussp. nov. is proposed.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Changying
authors.initials: C
authors.lastname: Ruan
authors.affiliation: China Center for Type Culture Collection, Wuhan, 430071, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Hong