title: Proteome Profiling and Ultrastructural Characterization of the Human RCMH Cell Line: Myoblastic Properties and Suitability for Myopathological Studies.
abstract: Studying (neuro)muscular disorders is a major topic in biomedicine with a demand for suitable model systems. Continuous cell culture (in vitro) systems have several technical advantages over in vivo systems and became widely used tools for discovering physiological/pathophysiological mechanisms in muscle. In particular, myoblast cell lines are suitable model systems to study complex biochemical adaptations occurring in skeletal muscle and cellular responses to altered genetic/environmental conditions. Whereas most in vitro studies use extensively characterized murine C2C12 cells, a comprehensive description of an equivalent human cell line, not genetically manipulated for immortalization, is lacking. Therefore, we characterized human immortal myoblastic RCMH cells using scanning (SEM) and transmission electron microscopy (TEM) and proteomics. Among more than 6200 identified proteins we confirm the known expression of proteins important for muscle function. Comparing the RCMH proteome with two well-defined nonskeletal muscle cells lines (HeLa, U2OS) revealed a considerable enrichment of proteins important for muscle function. SEM/TEM confirmed the presence of agglomerates of cytoskeletal components/intermediate filaments and a prominent rough ER. In conclusion, our results indicate RMCH as a suitable in vitro model for investigating muscle function-related processes such as mechanical stress burden and mechanotransduction, EC coupling, cytoskeleton, muscle cell metabolism and development, and (ER-associated) myopathic disorders.
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften, ISAS e.V. , Otto-Hahn-Str. 6b, 44227 Dortmund, Germany.
authors.forename: Laxmikanth
authors.initials: L
authors.lastname: Kollipara
authors.affiliation: Institute of Neuropathology, RWTH Aachen University Hospital , Pauwelsstrasse 30, D-52074 Aachen, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Buchkremer
authors.affiliation: Institute of Neuropathology, RWTH Aachen University Hospital , Pauwelsstrasse 30, D-52074 Aachen, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Weis
authors.affiliation: Institute of Neuropathology, RWTH Aachen University Hospital , Pauwelsstrasse 30, D-52074 Aachen, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Brauers
authors.affiliation: Electron Microscopic Facility, Institute of Pathology, RWTH Aachen University Hospital , D-52074 Aachen, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Hoss
authors.affiliation: Electron Microscopic Facility, Institute of Pathology, RWTH Aachen University Hospital , D-52074 Aachen, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Rütten
authors.affiliation: Programa de Farmacología Molecular y Clínica, ICBM, Facultad de Medicina, Universidad de Chile , Santiago 1058, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Caviedes
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften, ISAS e.V. , Otto-Hahn-Str. 6b, 44227 Dortmund, Germany.
authors.forename: René P
authors.initials: RP
authors.lastname: Zahedi
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften, ISAS e.V. , Otto-Hahn-Str. 6b, 44227 Dortmund, Germany.|Institute of Neuropathology, RWTH Aachen University Hospital , Pauwelsstrasse 30, D-52074 Aachen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Roos
mesh_terms: D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000473:pathology; D006801:Humans; D009135:Muscular Diseases / Q000378:metabolism / Q000473:pathology; D032446:Myoblasts / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome
keywords: RCMH; human muscle cell line; muscle proteomics; myoblasts; proteome profiling