title: Corynebacterium uropygiale sp. nov., isolated from the preen gland of Turkeys (Meleagris gallopavo).
abstract: A novel species of fastidious, lipophilic, club-shaped, Gram-positive bacteria was recovered from the preen glands of healthy Turkeys (Meleagris gallopavo) from two different locations. Phylogenetic analysis of the 16S rRNA gene showed highest similarity to Corynebacterium spheniscorum DSM 44757(T) (96.8%) with a 3.2kb stretch of rpoB sharing 82.4% sequence similarity to the same species. DNA fingerprinting by ERIC-PCR and polar lipid profiles clearly differentiated the Turkey isolates from the most closely related Corynebacteria, as did MALDI-TOF MS analysis. Chemotaxonomic tests revealed the presence of corynemycolic acids with C16:0, C18:0, C18:1ω9c and tuberculostearic acid as the major cellular fatty acids. The G+C content of the type strain was 60.7 mol%. The species was susceptible to ampicillin, kanamycin A, streptomycin, amikacin, polymyxin B and vancomycin. From our results, it becomes evident that the isolated organisms represent a new species, for which the name Corynebacterium uropygiale sp. nov. is proposed. The type strain is Iso10(T) (=DSM 46817(T)=LMG 28616(T)).
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany.
authors.forename: Markus Santhosh
authors.initials: MS
authors.lastname: Braun
authors.affiliation: Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University Hospital, INF 324, 69120 Heidelberg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Zimmermann
authors.affiliation: Department of Biophysical Chemistry, Heidelberg University, INF 253, 69120 Heidelberg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Danner
authors.affiliation: Molecular Biology Laboratory, Soil Science Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh 2202, Bangladesh.
authors.forename: Harun-or
authors.initials: HO
authors.lastname: Rashid
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany. Electronic address: wink@uni-heidelberg.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003352:Corynebacterium / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000662:veterinary*; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014422:Turkeys / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial susceptibility; DNA fingerprinting; MALDI-TOF MS; Phylogeny; Scanning electron microscopy; Uropygial gland