title: Hevea brasiliensis prohevein possesses a conserved C-terminal domain with amyloid-like properties in vitro.
abstract: Prohevein is a wound-induced protein and a main allergen from latex of Hevea brasiliensis (rubber tree). This 187 amino-acid protein is cleaved in two fragments: a N-terminal 43 amino-acids called hevein, a lectin bearing a chitin-binding motif with antifungal properties and a C-terminal domain (C-ter) far less characterized. We provide here new insights on the characteristics of prohevein, hevein and C-terminal domain. Using complementary biochemical (ThT/CR/chitin binding, agglutination) and structural (modeling, ATR-FTIR, TEM, WAXS) approaches, we show that this domain clearly displays all the characteristics of an amyloid-like proteins in vitro, that could confer agglutination activity in synergy with its chitin-binding activity. Additionally, this C-ter domain is highly conserved and present in numerous plant prohevein-like proteins or pathogenesis-related (PR and WIN) proteins. This could be the hallmark of the eventual presence of proteins with amyloid properties in plants, that could potentially play a role in defense through aggregation properties.
authors.affiliation: CNRS, CBMN, UMR 5248, Univ. Bordeaux, Bordeaux INP, F-33600 Pessac, France; CNRS, LCPO, UMR 5629, Univ. Bordeaux, Bordeaux INP, F-33600 Pessac, France. Electronic address: kberthelot@enscbp.fr.
authors.forename: Karine
authors.initials: K
authors.lastname: Berthelot
authors.affiliation: CNRS, CBMN, UMR 5248, Univ. Bordeaux, Bordeaux INP, F-33600 Pessac, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lecomte
authors.affiliation: CNRS, IBGC, UMR 5095, Univ. Bordeaux, F-33000 Bordeaux, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Coulary-Salin
authors.affiliation: CNRS, CRPP, UPR 8641, F-33600 Pessac, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Bentaleb
authors.affiliation: CNRS, LCPO, UMR 5629, Univ. Bordeaux, Bordeaux INP, F-33600 Pessac, France. Electronic address: peruch@enscbp.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Peruch
mesh_terms: D000371:Agglutination; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D052179:Antigens, Plant / Q000737:chemistry*; D017124:Conserved Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D000682:Amyloid; D052179:Antigens, Plant; C097982:Hev b 6.01 protein, Hevea brasiliensis; D010940:Plant Proteins
keywords: FTIR; Hevein; Latex allergen; Plant amyloid; Protein aggregation