title: [Diagnostic values of urinary netrin-1 and kidney injury molecule-1 for acute kidney injury induced by neonatal asphyxia].
abstract: OBJECTIVE
  To investigate the values of urinary netrin-1 and kidney injury molecule-1 (KIM-1) in the early diagnosis of acute kidney injury (AKI) induced by neonatal asphyxia.
  
  
  METHODS
  A total of 80 full-term neonates with asphyxia were enrolled (mild asphyxia: 34 neonates; severe asphyxia: 46 neonates). Forty normal full-term neonates were selected as the control group. Urinary samples were collected from the neonates in the three groups within 12 hours and 13-48 hours after birth. ELISA was applied to measure urinary levels of netrin-1 and KIM-1. Peripheral venous blood samples were also collected to measure serum creatinine (Scr) level.
  
  
  RESULTS
  Compared with the control group, the asphyxia group had significantly higher urinary levels of netrin-1 and KIM-1 within 48 hours after birth and a significantly higher Scr level within 13-48 hours after birth (P<0.05). The neonates in the AKI group had significantly higher urinary levels of netrin-1 and KIM-1 and Scr level within 48 hours after birth than those in the non-AKI group (P<0.05). The areas under the receiver operating characteristic curve for urinary netrin-1 and KIM-1 levels within 12 hours after birth to predict AKI after asphyxia were 0.878 (95% CI: 0.775-0.981; P<0.01) and 0.899 (95% CI: 0.829-0.969; P<0.01), respectively. Any two indicators of urinary netrin-1 level, urinary KIM-1 level, and Scr level within 12 hours after neonatal asphyxia had a positive correlation (P<0.05).
  
  
  CONCLUSIONS
  Urinary netrin-1 and KIM-1 levels increase significantly when neonates with asphyxia develop AKI. Urinary netrin-1 and KIM-1 can be used as indicators for the early diagnosis of AKI after asphyxia.
authors.affiliation: Department of Pediatrics, First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang 832000, China. zhr331619@sina.com.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Cao
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
mesh_terms: D058186:Acute Kidney Injury / Q000175:diagnosis* / Q000652:urine; D001238:Asphyxia Neonatorum / Q000150:complications*; D005260:Female; D000072596:Hepatitis A Virus Cellular Receptor 1; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008562:Membrane Glycoproteins / Q000652:urine*; D009414:Nerve Growth Factors / Q000652:urine*; D000075388:Netrin-1; D011991:Receptors, Virus; D025521:Tumor Suppressor Proteins / Q000652:urine*
chemical_list: C491143:HAVCR1 protein, human; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D011991:Receptors, Virus; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1