title: MicroRNA-499 Rs3746444 polymorphism and biliary atresia.
abstract: BACKGROUND
  Single nucleotide polymorphisms within microRNAs are known to affect the risk in development and prognosis of many diseases. This study was designed to investigate whether polymorphism of microRNA-499 (miR-499, rs3746444 A>G) is associated with risk to biliary atresia (BA).
  
  
  METHODS
  A hospital-based cases-control study was performed on a total of 507 Han Chinese (207 BA cases and 300 ethnically-matched healthy controls without any evidence of liver diseases) so as to analyze the association between miR-499 rs3746444 polymorphism and BA risk as well as liver function remission (LFR) after liver transplantation.
  
  
  RESULTS
  A significant higher frequency of the rs3746444 G alleles was found in the BA cases than the control group (odd ratio, 1.55, 95% confidence intervals [CIs], 1.15-2.10). This polymorphism was also observed to correlate with some clinic-pathological features of BA cases such as liver inflammatory. Further research found both higher levels of IL-6 (P<0.05) and TNF-α (P<0.05) in removed liver as well as in serum. What is more, the miR-499 rs3746444 polymorphism significantly affected the status of LFR (hazard ratio, 1.37; 95% CI, 1.08-1.83).
  
  
  CONCLUSIONS
  MiR-499 (rs3746444) gene polymorphisms may be genetic determinants for increased risk of BA and prolonged recovery of BA patients after liver transplantation in Han Chinese.
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: 1474229057@qq.com.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Joint Molecular Rheumatology Laboratory of the Institute of Health Sciences and Shanghai Renji Hospital, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, and Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: longerhans@163.com.
authors.forename: Longzhi
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Surgery, Shanghai Children's Medical Centre, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: chenqiminok@gmail.com.
authors.forename: Qimin
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: zhangjianjun@medmail.com.cn.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: longxidai@163.com.
authors.forename: Xidai
authors.initials: X
authors.lastname: Long
authors.affiliation: Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: xiaqiang@shsmu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001656:Biliary Atresia / Q000235:genetics* / Q000601:surgery*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000097:blood; D053208:Kaplan-Meier Estimate; D016031:Liver Transplantation; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D015999:Multivariate Analysis; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016016:Proportional Hazards Models; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; C548683:MIRN499 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha
keywords: Biliary atresia; MiR-499; Pediatric liver transplant; Polymorphism