title: Isolation and characterization of polymorphic EST-SSR and genomic SSR markers in spotted mandarin fish (Siniperca scherzeri Steindachne).
abstract: Spotted mandarin fish (Siniperca scherzeri Steindachne) feed solely on live fry of other fish species once the fry start feeding in the wild. In the present study, 26 polymorphic transcriptome-derived simple sequence repeat (SSR) markers and 14 genomic SSR markers were developed and characterized in S. scherzeri Steindachne by combining a biotin-enrichment protocol and transcriptome of F1 interspecies hybrids between S. chuatsi (♀) and S. scherzeri (♂). These 40 polymorphic SSRs amplified 168 alleles (mean 4.2). The number of alleles, observed heterozygosity, expected heterozygosity, and polymorphic information content per locus were in the range of 2 to 7 (mean 4.3), 0.1111 to 1.000 (mean 0.6718), 0.3118 to 0.8276 (mean 0.6901), and 0.2735 to 0.7902 (mean 0.6298), respectively. Ten of these microsatellite loci deviated significantly from Hardy-Weinberg equilibrium (P < 0.00125) after Bonferroni correction for multiple tests and no significant linkage disequilibrium (P < 0.00006) was observed. The microsatellite markers characterized from S. scherzeri could be a valuable tool in genetic evaluation for conservation and for assessment of the mechanism associated with unique food preference of S. scherzeri from a genetic point of view.
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China, and Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Dou
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China, and Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Liang
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China, and Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China, and Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Tian
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China, and Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors.forename: S
authors.initials: S
authors.lastname: He
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China, and Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Guo
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D002678:Chimera / Q000235:genetics; D020224:Expressed Sequence Tags; D005247:Feeding Behavior; D005260:Female; D056426:Genetic Loci; D005819:Genetic Markers; D005828:Genetics, Population; D006579:Heterozygote; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D010473:Perciformes / Q000235:genetics* / Q000254:growth & development; D011110:Polymorphism, Genetic; D059467:Transcriptome
chemical_list: D005819:Genetic Markers