title: Permissive Sense and Antisense Transcription from the 5' and 3' Long Terminal Repeats of Human T-Cell Leukemia Virus Type 1.
abstract: Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus, and, as such, its genome becomes chromosomally integrated following infection. The resulting provirus contains identical 5' and 3' peripheral long terminal repeats (LTRs) containing bidirectional promoters. Antisense transcription from the 3' LTR regulates expression of a single gene, hbz, while sense transcription from the 5' LTR controls expression of all other viral genes, including tax. Both the HBZ and Tax proteins are implicated in the development of adult T-cell leukemia (ATL), a T-cell malignancy caused by HTLV-1 infection. However, these proteins appear to harbor opposing molecular functions, indicating that they may act independently and at different time points prior to leukemogenesis. Here, we used bidirectional reporter constructs to test whether transcriptional interference serves as a mechanism that inhibits simultaneous expression of Tax and HBZ. We found that sense transcription did not interfere with antisense transcription from the 3' LTR and vice versa, even with strong transcription emanating from the opposing direction. Therefore, bidirectional transcription across the provirus might not restrict hbz or tax expression. Single-cell analyses revealed that antisense transcription predominates in the absence of Tax, which transactivates viral sense transcription. Interestingly, a population of Tax-expressing cells exhibited antisense but not activated sense transcription. Consistent with the ability of Tax to induce cell cycle arrest, this population was arrested in G(0)/G(1) phase. These results imply that cell cycle arrest inhibits Tax-mediated activation of sense transcription without affecting antisense transcription, which may be important for long-term viral latency.
  
  
  OBJECTIVE
  The chromosomally integrated form of the retrovirus human T-cell leukemia virus type 1 (HTLV-1) contains identical DNA sequences, known as long terminal repeats (LTRs), at its 5' and 3' ends. The LTRs modulate transcription in both forward (sense) and reverse (antisense) directions. We found that sense transcription from the 5' LTR does not interfere with antisense transcription from the 3' LTR, allowing viral genes encoded on opposite DNA strands to be simultaneously transcribed. Two such genes are tax and hbz, and while they are thought to function at different times during the course of infection to promote leukemogenesis of infected T cells, our results indicate that they can be simultaneously transcribed. We also found that the ability of Tax to induce cell cycle arrest inhibits its fundamental function of activating viral sense transcription but does not affect antisense transcription. This regulatory mechanism may be important for long-term HTLV-1 infection.
authors.affiliation: Brody School of Medicine, Department of Microbiology and Immunology, East Carolina University, Greenville, North Carolina, USA sylvain.laverdure@nih.gov lemassoni@ecu.edu.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Laverdure
authors.affiliation: Brody School of Medicine, Department of Microbiology and Immunology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Polakowski
authors.affiliation: Brody School of Medicine, Department of Microbiology and Immunology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Kimson
authors.initials: K
authors.lastname: Hoang
authors.affiliation: Brody School of Medicine, Department of Microbiology and Immunology, East Carolina University, Greenville, North Carolina, USA sylvain.laverdure@nih.gov lemassoni@ecu.edu.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lemasson
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic; D020079:Terminal Repeat Sequences; D014158:Transcription, Genetic; D014779:Virus Replication