title: In Vitro Cultivars of Vaccinium corymbosum L. (Ericaceae) are a Source of Antioxidant Phenolics.
abstract: The antioxidant activity and phenolic composition of six in vitro cultured blueberry seedlings were determined. Extracts were prepared in 85% ethanol from 30 days old in vitro cultured plants and used to evaluate the antioxidant capacities that included Ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazin (DPPH•) scavenging ability, total polyphenols (TP) and the partial phenolic composition performed by high performance liquid chromatography with diode array detector (HPLC-DAD), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS (ESI-QqQ)). All ethanolic extracts from in vitro blueberry cultivars displayed antioxidant activity, with Legacy, Elliott and Bluegold cultivars being the most active. In addition, we observed a positive correlation between phenolic content and antioxidant activity. Our results suggest that the antioxidant activity of the extracts is related to the content of chlorogenic acid myricetin, syringic acid and rutin, and tissue culture of blueberry seedlings is a good tool to obtain antioxidant extracts with reproducible profile of compounds.
authors.affiliation: Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile. L. B. O'Higgins Ave. #3363, Estación Central, Santiago of Chile, 9170022, Chile. rodrigo.contrerasar@usach.cl.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Contreras
authors.affiliation: Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile. L. B. O'Higgins Ave. #3363, Estación Central, Santiago of Chile, 9170022, Chile. hans.kohler@usach.cl.
authors.forename: Hans
authors.initials: H
authors.lastname: Köhler
authors.affiliation: Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile. L. B. O'Higgins Ave. #3363, Estación Central, Santiago of Chile, 9170022, Chile. marisol.pizarror@usach.cl.
authors.forename: Marisol
authors.initials: M
authors.lastname: Pizarro
authors.affiliation: Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile. L. B. O'Higgins Ave. #3363, Estación Central, Santiago of Chile, 9170022, Chile. gustavo.zuniga@usach.cl.
authors.forename: Gustavo E
authors.initials: GE
authors.lastname: Zúiga
keywords: DPPH• and FRAP; HPLC-DAD and LC-MS/MS; high-bush blueberry in vitro cultivars; phenolic composition