title: Down-regulation apoptosis signal-regulating kinase 1 gene reduced the Litopenaeus vannamei hemocyte apoptosis in WSSV infection.
abstract: Apoptosis signal-regulating kinase 1 (ASK1), a mitogen-activated protein kinase kinase kinase, is crucial in various cellular responses. In the present study, we identified and characterized an ASK1 homolog from Litopenaeus vannamei (LvASK1). The full-length cDNA of LvASK1 was 5400 bp long, with an open reading frame encoding a putative 1420 amino acid protein. LvASK1 was highly expressed in muscle, hemocyte, eyestalk and heart. Real-time RT-PCR analysis showed that the expression of the LvASK1 was upregulated during the white spot syndrome virus (WSSV) challenge. The knocked-down expression of LvASK1 by RNA interference significantly reduced the apoptotic ratio of the hemocytes collected from WSSV-infected L. vannamei. Furthermore, the down-regulation of LvASK1 also decreased the cumulative mortality of WSSV-infected L. vannamei. These results suggested that down-regulation of LvASK1 decreased the apoptotic rate of hemocytes in WSSV-infected shrimp, and that it could contribute to the reduction of cumulative mortality in WSSV-infected L. vannamei.
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Yong-Gui
authors.initials: YG
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Ze-Zhi
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Yue
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Bi
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Weng
authors.affiliation: Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China; School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China. Electronic address: chenyh6@mail.sysu.edu.cn.
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000502:physiology; D048848:MAP Kinase Kinase Kinase 5 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics / Q000276:immunology / Q000502:physiology* / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D048848:MAP Kinase Kinase Kinase 5
keywords: Apoptosis; Apoptosis signal-regulating kinase 1; Litopenaeus vannamei; White spot syndrome virus