title: Upregulated Pdx1 and MafA Contribute to β-Cell Function Improvement by Sleeve Gastrectomy.
abstract: OBJECTIVE
  Sleeve gastrectomy is an effective technique for the treatment of severe obesity, and its effects on the improved β-cell function have not yet been fully understood.
  
  
  METHODS
  From February 2014 to July 2015, sleeve gastrectomy was performed in 5 patients with T2D, who were assessed before and after sleeve gastrectomy (SG). Moreover, a high-fat-diet (HFD) mouse model was also used to study the molecular mechanisms of β-cell functional improvement after SG.
  
  
  RESULTS
  The glucose-stimulated acute insulin response was restored in the T2D patients after SG. The expression of GLP-1 in colonic tissue as well as β-cell specific transcription factors (TFs), Pdx1, and MafA in islets was significantly increased after SG.
  
  
  CONCLUSIONS
  β-cell dysfunction can be ameliorated by SG. The re-activation of key TFs contributes to the improvement of β cell function.
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, Shanghai, 200072, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, Shanghai, 200072, China.
authors.forename: Han
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, Shanghai, 200072, China.
authors.forename: Chunjun
authors.initials: C
authors.lastname: Sheng
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, Shanghai, 200072, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, Shanghai, 200072, China.
authors.forename: Kexiu
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, Shanghai, 200072, China. qushencn@hotmail.com.
authors.forename: Shen
authors.initials: S
authors.lastname: Qu
mesh_terms: D000368:Aged; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism* / Q000601:surgery; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005743:Gastrectomy; D052216:Glucagon-Like Peptide 1 / Q000096:biosynthesis; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D051473:Maf Transcription Factors, Large / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009767:Obesity, Morbid / Q000150:complications / Q000378:metabolism / Q000601:surgery*; D015534:Trans-Activators / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D018398:Homeodomain Proteins; C485159:MAFA protein, human; D051473:Maf Transcription Factors, Large; D015534:Trans-Activators; D014157:Transcription Factors; C084010:pancreatic and duodenal homeobox 1 protein; D052216:Glucagon-Like Peptide 1
keywords: GLP-1; MafA; Pancreatic β cell; Pdx1; Sleeve gastrectomy