title: Utility and diagnostic performance of Mycobacterium tuberculosis complex by two immunochromatographic assays as compared with the molecular Genotype assay in Nigeria.
abstract: Among the disadvantages of smear microscopy for detection of tuberculosis cases is its inability to differentiate between Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM). This study evaluated two, new immunochromatographic assays - Capilia TB-Neo and SD Bioline - on unheated and heated cultures at 80°C for 30min respectively for their ability to discriminate between MTB complex and NTM as compared with the molecular Genotype assay. Mycobacteria used in the study were obtained from smear-positive specimens collected from patients at four major hospitals in Cross River State, Nigeria. Capilia TB-Neo and SD Bioline showed sensitivities of 98.8% and 93.8% respectively and 100% specificity for both assays. Heating the isolates did not significantly impact the test performance. Both tests are recommended for use in rapid differentiation of strains isolated in Nigeria.
authors.affiliation: Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Cameroon. Electronic address: thumamo@yahoo.fr.
authors.forename: Benjamin Thumamo
authors.initials: BT
authors.lastname: Pokam
authors.affiliation: Department of Medical Laboratory Science, College of Medical Sciences, University of Calabar, Nigeria.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Asuquo
authors.affiliation: WHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, 97183 Abymes, Guadeloupe, France.
authors.forename: Khye Seng
authors.initials: KS
authors.lastname: Goh
authors.affiliation: Department of Medical Laboratory Science, College of Medical Sciences, University of Calabar, Nigeria.
authors.forename: Lydia N
authors.initials: LN
authors.lastname: Abia-Bassey
authors.affiliation: WHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, 97183 Abymes, Guadeloupe, France.
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
keywords: Capilia TB-Neo; Heated strains; Mycobacterium tuberculosis complex; Non-tuberculous mycobacteria; SD Bioline