title: Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts.
abstract: Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA.
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France. fakcha@ifremer.fr.
authors.forename: F
authors.initials: F
authors.lastname: Akcha
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.
authors.forename: A
authors.initials: A
authors.lastname: Barranger
authors.affiliation: Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France.
authors.forename: E
authors.initials: E
authors.lastname: Bachère
authors.affiliation: Unité Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Université de Caen Normandie, Sorbonne Universités, Muséum national d'Histoire naturelle, Université Pierre et Marie Curie, CNRS, IRD, 57 rue Cuvier, 75005, Paris, France.
authors.forename: C Heude
authors.initials: CH
authors.lastname: Berthelin
authors.affiliation: Acobiom, 1682 rue de la Valsière, CS 77394 Cap Delta Biopole Euromédecine II, 34184, Montpellier Cedex 04, France.
authors.forename: D
authors.initials: D
authors.lastname: Piquemal
authors.affiliation: Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France.
authors.forename: P
authors.initials: P
authors.lastname: Alonso
authors.affiliation: Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France.
authors.forename: R Rondon
authors.initials: RR
authors.lastname: Sallan
authors.affiliation: IDAEA-CSIC, Environmental Chemistry Department, C/ Jordi Girona, 1808034, Barcelona, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Dimastrogiovanni
authors.affiliation: IDAEA-CSIC, Environmental Chemistry Department, C/ Jordi Girona, 1808034, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Porte
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.
authors.forename: D
authors.initials: D
authors.lastname: Menard
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.
authors.forename: A
authors.initials: A
authors.lastname: Szczybelski
authors.affiliation: Ifremer, Laboratoire de Génétique et Pathologies, Rue de Mus de Loup, La Tremblade, 17390, France.
authors.forename: A
authors.initials: A
authors.lastname: Benabdelmouna
authors.affiliation: Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS/IRD/IFREMER, rue Dumont d'Urville, technopôle, Brest-Iroise, 29280, Plouzané, France.
authors.forename: M
authors.initials: M
authors.lastname: Auffret
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.
authors.forename: J
authors.initials: J
authors.lastname: Rouxel
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.
authors.forename: T
authors.initials: T
authors.lastname: Burgeot
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D004237:Diuron / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D005718:Gametogenesis / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006434:Hemocytes / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D014874:Water Pollutants, Chemical; D004237:Diuron
keywords: Gene expression; Histology; Immunity; Oyster; Pesticide; RT-qPCR; Reproduction; Steroid