title: Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells.
abstract: Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer-Emmett-Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett-Joyner-Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film. Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95-53% after 24h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.
authors.affiliation: Nanobiomaterials Laboratory, Institute of Applied Biotechnology (CINDEFI, UNLP-CONICET-CCT La Plata), Department of Chemistry, School of Sciences, Universidad Nacional de La Plata, Calle 47 y115, CP 1900 La Plata, Argentina.
authors.forename: Maximiliano
authors.initials: M
authors.lastname: L Cacicedo
authors.affiliation: Chemical Inorganic Center (CEQUINOR, UNLP, CONICET), School of Sciences, Universidad Nacional de La Plata (UNLP), Calle 47 y 115, (1900) La Plata, Argentina.
authors.forename: Ignacio
authors.initials: I
authors.lastname: E León
authors.affiliation: CoMP (Composite Materials Group), Research Institute of Material Science and Technology (INTEMA), Engineering School, National University of Mar del Plata, Solis 7575, (B7608FDQ) Mar del Plata, Argentina.
authors.forename: Jimena
authors.initials: J
authors.lastname: S Gonzalez
authors.affiliation: Integrated Technologies Laboratory (InteLAB), CTC/EQA, Universidad Federal de Santa Catarina, Florianopolis, Brazil.
authors.forename: Luismar
authors.initials: L
authors.lastname: M Porto
authors.affiliation: CoMP (Composite Materials Group), Research Institute of Material Science and Technology (INTEMA), Engineering School, National University of Mar del Plata, Solis 7575, (B7608FDQ) Mar del Plata, Argentina.
authors.forename: Vera
authors.initials: V
authors.lastname: A Alvarez
authors.affiliation: Nanobiomaterials Laboratory, Institute of Applied Biotechnology (CINDEFI, UNLP-CONICET-CCT La Plata), Department of Chemistry, School of Sciences, Universidad Nacional de La Plata, Calle 47 y115, CP 1900 La Plata, Argentina. Electronic address: grcastro@gmail.com.
authors.forename: Guillermo R
authors.initials: GR
authors.lastname: Castro
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D065546:Drug Liberation; D041821:Gluconacetobacter / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D019073:HT29 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000464:Alginates; D000903:Antibiotics, Antineoplastic; D006603:Hexuronic Acids; D004317:Doxorubicin; D020723:Glucuronic Acid; D002482:Cellulose
keywords: Alginate; Bacterial cellulose; Cancer therapy; Doxorubicin; Drug delivery; Human colorectal HT-29 cells; Nanocomposite