title: Characterization of Rebound Depolarization in Neurons of the Rat Medial Geniculate Body In Vitro.
abstract: Rebound depolarization (RD) is a response to the offset from hyperpolarization of the neuronal membrane potential and is an important mechanism for the synaptic processing of inhibitory signals. In the present study, we characterized RD in neurons of the rat medial geniculate body (MGB), a nucleus of the auditory thalamus, using whole-cell patch-clamp and brain slices. RD was proportional in strength to the duration and magnitude of the hyperpolarization; was effectively blocked by Ni(2+) or Mibefradil; and was depressed when the resting membrane potential was hyperpolarized by blocking hyperpolarization-activated cyclic nucleotide-gated (HCN) channels with ZD7288 or by activating G-protein-gated inwardly-rectifying K(+) (GIRK) channels with baclofen. Our results demonstrated that RD in MGB neurons, which is carried by T-type Ca(2+) channels, is critically regulated by HCN channels and likely by GIRK channels.
authors.affiliation: CAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
authors.forename: Xin-Xing
authors.initials: XX
authors.lastname: Wang
authors.affiliation: Auditory Research Laboratory, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Physiology, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Physiology, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.|Auditory Research Laboratory, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China. linchen@ustc.edu.cn.|Auditory Research Laboratory, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China. linchen@ustc.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D020747:Calcium Channels, T-Type / Q000502:physiology*; D005260:Female; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000502:physiology*; D005829:Geniculate Bodies / Q000502:physiology*; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020747:Calcium Channels, T-Type; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
keywords: Brain slice; GIRK channel; HCN channel; Medial geniculate body; Rebound depolarization; Resting membrane potential; T-type calcium channel