title: Immobilization of Lactobacillus rhamnosus in mesoporous silica-based material: An efficiency continuous cell-recycle fermentation system for lactic acid production.
abstract: Lactic acid bacteria immobilization methods have been widely used for lactic acid production. Until now, the most common immobilization matrix used is calcium alginate. However, Ca-alginate gel disintegrated during lactic acid fermentation. To overcome this deficiency, we developed an immobilization method in which Lactobacillus rhamnosus cells were successfully encapsulated into an ordered mesoporous silica-based material under mild conditions with a high immobilization efficiency of 78.77% by using elemental analysis. We also optimized the cultivation conditions of the immobilized L. rhamnosus and obtained a high glucose conversion yield of 92.4%. Furthermore, L. rhamnosus encapsulated in mesoporous silica-based material exhibited operational stability during repeated fermentation processes and no decrease in lactic acid production up to 8 repeated batches.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China; State Key Laboratory of Theoretical and Computational Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: The First Hospital of Jilin University, 71 Xinmin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Xie
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Xuedun
authors.initials: X
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Theoretical and Computational Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Xuri
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130021, Jilin Province, PR China. Electronic address: lzq@jlu.edu.cn.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D000464:Alginates / Q000737:chemistry; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D005285:Fermentation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019344:Lactic Acid / Q000096:biosynthesis*; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry* / Q000378:metabolism*; D059027:Recycling; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D019344:Lactic Acid; D012822:Silicon Dioxide; D020723:Glucuronic Acid
keywords: Ca-alginate; Cell immobilization; Lactic acid production; Lactobacillus rhamnosus; Mesoporous silica-based material