title: A new combined green method for 2-Chlorophenol removal using cross-linked Brassica rapa peroxidase in silicone oil.
abstract: This study proposes a new technique to treat waste air containing 2-Chlorophenol (2-CP), namely an integrated process coupling absorption of the compound in an organic liquid phase and its enzymatic degradation. Silicone oil (47V20) was used as an organic absorbent to allow the volatile organic compound (VOC) transfer from the gas phase to the liquid phase followed by its degradation by means of Cross-linked Brassica rapa peroxidase (BRP) contained in the organic phase. An evaluation of silicone oil (47V20) absorption capacity towards 2-CP was first accomplished by determining its partition coefficient (H) in this solvent. The air-oil partition coefficient of 2-CP was found equal to 0.136 Pa m(3) mol(-1), which is five times lower than the air-water value (0.619 Pam(3) mol(-1)). The absorbed 2-CP was then subject to enzymatic degradation by cross-linked BRP aggregates (BRP-CLEAs). The degradation step was affected by four parameters (contact time; 2-CP, hydrogen peroxide and enzyme concentrations), which were optimized in order to obtain the highest conversion yield. A maximal conversion yield of 69% and a rate of 1.58 mg L(-1) min(-1)were obtained for 100 min duration time when 2-CP and hydrogen peroxide concentrations were respectively 80 mg L(-1) and 6 mM in the presence of 2.66 UI mL(-1) BRP-CLEAs. The reusability of BRP-CLEAs in silicone oil was assessed, showing promising results since 59% of their initial efficiency remained after three batches.
authors.affiliation: Laboratoire de Biomatériaux et Phénomènes de Transport, Faculté des Sciences et de la Technologie, Université Yahia Fares de Médéa, Pole Universitaire, RN1, Médéa 26000 Algeria; Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France. Electronic address: nassima_2900@yahoo.fr.
authors.forename: Nassima
authors.initials: N
authors.lastname: Tandjaoui
authors.affiliation: Laboratoire de Biomatériaux et Phénomènes de Transport, Faculté des Sciences et de la Technologie, Université Yahia Fares de Médéa, Pole Universitaire, RN1, Médéa 26000 Algeria; Laboratoire de Génie de la Réaction, Faculté de Génie Mécanique et Génie des Procédés, Université Houari Boumediene, Bab Ezzouar, Alger 16111, Algeria.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Abouseoud
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France; Université européenne de Bretagne, 5 Boulevard Laennec, 35000 Rennes, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Couvert
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France; Université européenne de Bretagne, 5 Boulevard Laennec, 35000 Rennes, France.
authors.forename: Abdeltif
authors.initials: A
authors.lastname: Amrane
authors.affiliation: Laboratoire de Génie de la Réaction, Faculté de Génie Mécanique et Génie des Procédés, Université Houari Boumediene, Bab Ezzouar, Alger 16111, Algeria.
authors.forename: Amina
authors.initials: A
authors.lastname: Tassist
mesh_terms: D000327:Adsorption; D000393:Air Pollutants / Q000302:isolation & purification*; D029741:Brassica rapa / Q000201:enzymology*; D002384:Catalysis; D002733:Chlorophenols / Q000302:isolation & purification*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry*; D012827:Silicone Oils / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000302:isolation & purification*
chemical_list: D000393:Air Pollutants; D002733:Chlorophenols; D003432:Cross-Linking Reagents; D012827:Silicone Oils; D055549:Volatile Organic Compounds; D006861:Hydrogen Peroxide; D009195:Peroxidase; C030683:2-chlorophenol; D005976:Glutaral
keywords: 2-Chlorophenol; Absorption; Brassica rapa peroxidase; CLEAs; Enzymatic degradation; VOC treatment