title: The chemical coding of 5-hydroxytryptamine containing enteroendocrine cells in the mouse gastrointestinal tract.
abstract: The majority of 5-HT (serotonin) in the body is contained in enteroendocrine cells of the gastrointestinal mucosa. From the time of their discovery over 80 years ago, the 5-HT-containing cells have been regarded as a class of cell that is distinct from enteroendocrine cells that contain peptide hormones. However, recent studies have cast doubt on the concept of there being distinct classes of enteroendocrine cells, each containing a single hormone or occasionally more than one hormone. Instead, data are rapidly accumulating that there are complex patterns of colocalisation of hormones that identify multiple subclasses of enteroendocrine cells. In the present work, multiple labelling immunohistochemistry is used to investigate patterns of colocalisation of 5-HT with enteric peptide hormones. Over 95 % of 5-HT cells in the duodenum also contained cholecystokinin and about 40 % of them also contained secretin. In the jejunum, about 75 % of 5-HT cells contained cholecystokinin but not secretin and 25 % contained 5-HT plus both cholecystokinin and secretin. Small proportions of 5-HT cells contained gastrin or somatostatin in the stomach, PYY or GLP-1 in the small intestine and GLP-1 or somatostatin in the large intestine. Rare or very rare 5-HT cells contained ghrelin (stomach), neurotensin (small and large intestines), somatostatin (small intestine) and PYY (in the large intestine). It is concluded that 5-HT-containing enteroendocrine cells are heterogeneous in their chemical coding and by implication in their functions.
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Reynaud
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Josiane
authors.initials: J
authors.lastname: Fakhry
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Linda
authors.initials: L
authors.lastname: Fothergill
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Brid
authors.initials: B
authors.lastname: Callaghan
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Ringuet
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Billie
authors.initials: B
authors.lastname: Hunne
authors.affiliation: InVivo Animal Nutrition & Health, Talhouët, 56250, Saint-Nolff, France.
authors.forename: David M
authors.initials: DM
authors.lastname: Bravo
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia. j.furness@unimelb.edu.au.|Florey Institute of Neuroscience and Mental Health, Parkville, VIC, 3010, Australia. j.furness@unimelb.edu.au.
authors.forename: John B
authors.initials: JB
authors.lastname: Furness
mesh_terms: D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism; D019858:Enteroendocrine Cells / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000166:cytology*; D054439:Ghrelin / Q000378:metabolism; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism; D007150:Immunohistochemistry; D008810:Mice, Inbred C57BL; D009496:Neurotensin / Q000378:metabolism; D019894:Peptide YY / Q000378:metabolism; D012633:Secretin / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism
chemical_list: D005755:Gastrins; D054439:Ghrelin; D019894:Peptide YY; D012633:Secretin; D012701:Serotonin; D009496:Neurotensin; D013004:Somatostatin; D052216:Glucagon-Like Peptide 1; D002766:Cholecystokinin
keywords: 5-hydroxytryptamine enterochromaffin cells; Cholecystokinin; Enteroendocrine cells; Gastrointestinal hormones; Secretin