title: Correlation of elution and sensitivity of cell lines to dental composites.
abstract: OBJECTIVE
  The aim of the present study was to understand the role played by composition on the elution of dental composites and the subsequent cytotoxicity, the sensitivity of different cell lines to eluates on a temporal basis and the correlation of the two parameters elution and cytotoxicity.
  
  
  METHODS
  LC-MS was done to analyze the eluates. MTT assay was done to assess cytotoxicity on two cell lines.
  
  
  RESULTS
  Eluates were found to have matrix monomers, photoinitiators and their degradation products. The short-term viability of other mammalian cell line was inferior to human cell line. However human cell line became more sensitive to long-term incubation with composites. There was a strong inverse correlation to elution of monomers and photoinitiators and cell viability for both cell lines. Bisphenol A elution did not correlate to cell viability.
  
  
  CONCLUSIONS
  Other mammalian cell lines may be more sensitive to acute toxin build-up than human cell line while the latter may be more sensitive to prolonged toxin exposure. Dimethacrylate based composites elute more and exert strong cytotoxicity than Ormocer and Silorane based composites. Most of the eluates correlated linearly to cytotoxicity.
authors.affiliation: Rehabilitation Bio Engineering Group, Engineering Design Department, IITM, Chennai 600036, India. Electronic address: drsusila2000@yahoo.com.
authors.forename: Anand V
authors.initials: AV
authors.lastname: Susila
authors.affiliation: Rehabilitation Bio Engineering Group, Engineering Design Department, IITM, Chennai 600036, India.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balasubramanian
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003188:Composite Resins / Q000737:chemistry / Q000633:toxicity*; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity*; D063225:Organically Modified Ceramics / Q000737:chemistry / Q000633:toxicity; D062047:Silorane Resins / Q000737:chemistry / Q000633:toxicity
chemical_list: D001672:Biocompatible Materials; C075816:Ceram-Fil; D003188:Composite Resins; D005899:Glass Ionomer Cements; D008689:Methacrylates; D063225:Organically Modified Ceramics; C000621294:Restofill; D062047:Silorane Resins; C544925:filtek P90
keywords: BPA; Biocompatibility: Dental composites; BisGMA; Dimethacrylate; Monomer; Ormocer; Photoinitiator; Silorane