title: Dual-label quantum dot-based immunoassay for simultaneous determination of Carbadox and Olaquindox metabolites in animal tissues.
abstract: A novel and reliable dual-label direct competitive fluorescence-linked immunosorbent assay (dc-FLISA) based on quantum dots (QDs) was developed for the simultaneous determination of the major metabolites of Carbadox and Olaquindox residues in animal tissues, using anti-QCA monoclonal antibodies and anti-MQCA polyclonal antibodies labeled with QD520 and QD635, respectively. The limits of detection for QCA and MQCA were 0.05 and 0.07ng/ml, respectively. The method was used to analyze fortified samples and analyte recoveries ranged from 81.5% to 98.2% (QCA) and 84.2% to 95.7% (MQCA). Good correlations between the dc-FLISA method and HPLC were demonstrated for the determination of QCA and MQCA residues in swine tissue samples, confirming the reliability of the proposed method.
authors.affiliation: College of Life Science, Chongqing Normal University, Chongqing 401331, PR China. Electronic address: hnxylt@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Le
authors.affiliation: College of Veterinary, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Liqian
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Wuhan Institute of Animal Health Supervision, Wuhan 430012, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D002218:Carbadox / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015200:Fluoroimmunoassay / Q000379:methods*; D045663:Quantum Dots; D011810:Quinoxalines / Q000378:metabolism*; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D011810:Quinoxalines; C013168:olaquindox; D002218:Carbadox
keywords: Fluoroimmunoassay; Methyl-3-quinoxaline-2-carboxylic acid; Quantum dots; Quinoxaline-2-carboxylic acid; Simultaneous detection