title: Association studies on the bovine lipoprotein lipase gene polymorphism with growth and carcass quality traits in Qinchuan cattle.
abstract: Lipoprotein lipase (LPL) is considered as an essential enzyme in lipid deposition and tissue metabolism. It has been proposed to be a lead candidate gene for genetic markers of lipid deposition and energy balance. In this paper, polymorphisms in the LPL gene were investigated in 554 Chinese Qinchuan cattle by PCR-RFLP and DNA sequencing. Seven single nucleotide polymorphisms (SNPs) were identified, which included one mutation (g.91C > T) in the 5'untranslated region (UTR), four synonymous mutations (g.17015A > G, g.18362G > A, g.18377T > C and g.19873T > C) and two mutations (g.25225A > G and g.25316T > G) in the 3'UTR. The frequencies of SNP g.18377T > C and g.25316T > G were skewed from Hardy-Weinberg equilibrium in all the samples (chi-square test, P < 0.05). An association analysis showed that five loci (except for g.91C > T and g.18377T > C) were significantly correlated with some growth and carcass quality traits. These results demonstrate that LPL might be a potential candidate gene for marker-assisted selection (MAS).
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: 26660162@qq.com.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Gui
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: linga2177@163.com.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Jia
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: 827136244@qq.com.
authors.forename: Yaran
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: zhao.chunping@hotmail.com.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; National Beef Cattle Improvement Center of Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: zanlinsen@163.com.
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001835:Body Weight / Q000235:genetics; D002417:Cattle / Q000145:classification / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D005260:Female; D056726:Genetic Association Studies / Q000379:methods*; D015810:Linkage Disequilibrium; D008071:Lipoprotein Lipase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D008071:Lipoprotein Lipase
keywords: Carcass quality; Growth; LPL gene; Polymorphism