title: Identification of cervical cancer markers using cDNA subtraction approach.
abstract: OBJECTIVE
  Cervical cancer markers are not well known for accurate detection of the disease.
  
  
  METHODS
  In the present study, cervical cancer samples were collected from the 42 patients in the Department of Surgery and Medicine of Weifang People's Hospital and Medical College, Shandong, China. The cDNA subtraction approach was performed to find out the specific transcripts, which are responsible for cervical cancer. The specific differentially expressed transcripts were identified by Basic Local Alignment Search Tool (BLAST) analysis and the results were validated by immunohistochemistry.
  
  
  RESULTS
  Following differentially expressed specific transcripts, such as ID-1, Hif 1a, and the Y-box were usefully employed as a marker to accurately detect cervical cancer. CONCLUSION. The identified markers are promising in the accurate detection of cervical cancer in terms of its molecular basis and management of the disease.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: S H
authors.initials: SH
authors.lastname: Man
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: X Y
authors.initials: XY
authors.lastname: Ding
authors.forename: W L
authors.initials: WL
authors.lastname: Xiao
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis; D007150:Immunohistochemistry; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics; D051840:Y-Box-Binding Protein 1 / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human