title: Collection methods of trematode eggs using experimental animal models.
abstract: Although observing the eggs of human parasitic helminth is essential for medical education in parasitology, opportunities for collection of the eggs are limited. Collection of the eggs using experimental animal models is needed for a sustainable supply. The metacercariae of three trematode species, Paragonimus westermani, Clonorchis sinensis and Metagonimus yokogawai, were collected from the second intermediate hosts: freshwater crabs and fishes, which were obtained using online shopping in Japan, and inoculated to experimental animal rat and dog. Consequently, eggs of the three trematode species were obtained abundantly from the feces of the animals. The eggs are being used for student training in several Japanese universities. In this article, we introduce the collection procedures for trematode eggs.
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Tsubokawa
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Fusako
authors.initials: F
authors.lastname: Mikami
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Shibata
authors.affiliation: Department of Animal Risk Management, Faculty of Risk and Crisis Management, Chiba Institute of Science, 15-8 Shiomi, Choshi, Chiba 288-0025, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibahara
authors.affiliation: Center for Laboratory Animal Science, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukuda
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Shinzaburo
authors.initials: S
authors.lastname: Takamiya
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: tsujin@med.kitasato-u.ac.jp.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
keywords: Clonorchis sinensis; Metacercaria; Metagonimus yokogawai; Paragonimus westermani; Trematode eggs