title: Hypoxia-inducible miR-210 contributes to preeclampsia via targeting thrombospondin type I domain containing 7A.
abstract: Preeclampsia, a relatively common pregnancy disorder, is a major contributor to maternal mortality and morbidity worldwide. An elevation in microRNA-210 (miR-210) expression in the placenta has been reported to be associated with preeclampsia. Our bioinformatic analysis showed that thrombospondin type I domain containing 7A (THSD7A) is a predicted target for miR-210. The aim of this study was to determine whether miR-210 is involved in preeclampsia through its targeting of THSD7A in human placental trophoblasts. In preeclamptic placental tissues, THSD7A levels were significantly downregulated, and were inversely correlated with the levels of miR-210. THSD7A was validated as a direct target of miR-210 using quantitative real time PCR (qRT-PCR), Western blotting, and dual luciferase assays in HTR8/SVneo cells. Transwell insert invasion assays showed that THSD7A mediated the invasion-inhibitory effect of miR-210 in HTR8/SVneo cells. Interestingly, hypoxia markedly increased miR-210 expression while suppressing THSD7A expression in a time-dependent manner in HTR8/SVneo cells. This study provides novel data on the function of THSD7A in human placental cells, and extends our knowledge of how miR-210 is involved in the development of the preeclampsia.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|School of Life Sciences, Lanzhou University, Lanzhou 730000, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Rongcan
authors.initials: R
authors.lastname: Luo
authors.affiliation: Department of Obstetrics and Gynaecology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|College of Life Sciences, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Cao
authors.affiliation: Department of Obstetrics and Gynaecology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yangyu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Li
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada.
authors.forename: Chun
authors.initials: C
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000328:Adult; D015687:Cell Hypoxia; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000150:complications* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D015203:Reproducibility of Results; D019699:Thrombospondins / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism
chemical_list: C543800:MIRN210 microRNA, human; D035683:MicroRNAs; C546399:THSD7A protein, human; D019699:Thrombospondins