title: An alternative approach for assessment of liquid chromatography-mass spectrometry matrix effects using auto-sampler programmed co-injection.
abstract: We report the use of auto-sampler programmable functions to co-inject analyte standard solution and matrix extract to assess ion enhancement and suppression (matrix effects) in LC-MS. This is effectively an automated post-extraction addition (APEA) procedure, emulating the manual post-extraction addition (PEA) approach widely adopted for assessment of matrix effects. To verify that APEA was comparable to the conventional PEA approach, matrix effects were determined using both methods for a selection of 31 illicit and pharmaceutical drugs in 10 different human urine extracts. Matrix effects measured using APEA were statistically indistinguishable from manual PEA methodology for 27 of the 31 drugs. Of the four drugs that showed significant differences using the two methods, three differed by less than 2 %, which is within the expected accuracy limits required for matrix effect determinations. The remaining analyte, trimeprazine, was found to degrade in the spiked PEA matrix extract, accounting for the difference between matrix effects measured by the PEA and APEA approaches. APEA enables a single matrix extract to be assessed at multiple analyte concentrations, resulting in a considerable reduction in sample preparation time. In addition, APEA can reduce the quantity of analyte-free sample matrix required for matrix effect assessment, which is an important consideration in certain analytical and bioanalytical fields. This work shows that APEA may be considered as an acceptable alternative to PEA for the assessment of matrix effects in LC-MS method validation and may be applicable to a variety of matrices such as environmental samples.
authors.affiliation: School of Chemical and Physical Sciences, Flinders University, Sturt Road, Bedford Park, South Australia, 5042, Australia.
authors.forename: Caitlyn A
authors.initials: CA
authors.lastname: Rogers
authors.affiliation: School of Chemical and Physical Sciences, Flinders University, Sturt Road, Bedford Park, South Australia, 5042, Australia. peter.stockham@sa.gov.au.|Forensic Science SA, GPO Box 2790, Adelaide, South Australia, 5000, Australia. peter.stockham@sa.gov.au.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Stockham
authors.affiliation: Forensic Science SA, GPO Box 2790, Adelaide, South Australia, 5000, Australia.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Nash
authors.affiliation: School of Chemical and Physical Sciences, Flinders University, Sturt Road, Bedford Park, South Australia, 5042, Australia.
authors.forename: Sheridan M
authors.initials: SM
authors.lastname: Martin
authors.affiliation: Forensic Science SA, GPO Box 2790, Adelaide, South Australia, 5000, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Kostakis
authors.affiliation: School of Chemical and Physical Sciences, Flinders University, Sturt Road, Bedford Park, South Australia, 5042, Australia.
authors.forename: Claire E
authors.initials: CE
authors.lastname: Lenehan
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D013287:Illicit Drugs / Q000097:blood / Q000652:urine; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000652:urine*; D015813:Substance Abuse Detection / Q000295:instrumentation* / Q000379:methods
chemical_list: D013287:Illicit Drugs; D004364:Pharmaceutical Preparations
keywords: Ion enhancement; Ion suppression; Liquid chromatography mass spectrometry; Matrix effect; Post- extraction addition; Post-column infusion