title: Transcriptome dynamics of a susceptible wheat upon Fusarium head blight reveals that molecular responses to Fusarium graminearum infection fit over the grain development processes.
abstract: In many plant/pathogen interactions, host susceptibility factors are key determinants of disease development promoting pathogen growth and spreading in plant tissues. In the Fusarium head blight (FHB) disease, the molecular basis of wheat susceptibility is still poorly understood while it could provide new insights into the understanding of the wheat/Fusarium graminearum (Fg) interaction and guide future breeding programs to produce cultivars with sustainable resistance. To identify the wheat grain candidate genes, a genome-wide gene expression profiling was performed in the French susceptible wheat cultivar, Recital. Gene-specific two-way ANOVA of about 40 K transcripts at five grain developmental stages identified 1309 differentially expressed genes. Out of these, 536 were impacted by the Fg effect alone. Most of these Fg-responsive genes belonged to biological and molecular functions related to biotic and abiotic stresses indicating the activation of common stress pathways during susceptibility response of wheat grain to FHB. This analysis revealed also 773 other genes displaying either specific Fg-responsive profiles along with grain development stages or synergistic adjustments with the grain development effect. These genes were involved in various molecular pathways including primary metabolism, cell death, and gene expression reprogramming. An increasingly complex host response was revealed, as was the impact of both Fg infection and grain ontogeny on the transcription of wheat genes. This analysis provides a wealth of candidate genes and pathways involved in susceptibility responses to FHB and depicts new clues to the understanding of the susceptibility determinism in plant/pathogen interactions.
authors.affiliation: INRA, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France.|Université Blaise Pascal, UMR Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France.
authors.forename: Cherif
authors.initials: C
authors.lastname: Chetouhi
authors.affiliation: INRA, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France. ludovic.bonhomme@clermont.inra.fr.|Université Blaise Pascal, UMR Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France. ludovic.bonhomme@clermont.inra.fr.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Bonhomme
authors.affiliation: INRA, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France.|Université Blaise Pascal, UMR Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Lasserre-Zuber
authors.affiliation: INRA, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France.|Université Blaise Pascal, UMR Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Cambon
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences, Beaucouzé, F-49071, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pelletier
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences, Beaucouzé, F-49071, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Renou
authors.affiliation: INRA, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France. thierry.langin@clermont.inra.fr.|Université Blaise Pascal, UMR Genetics, Diversity and Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France. thierry.langin@clermont.inra.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Langin
mesh_terms: D060467:Disease Resistance; D004198:Disease Susceptibility; D002523:Edible Grain / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000382:microbiology; D005670:Fusarium / Q000254:growth & development* / Q000472:pathogenicity; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D018745:Genome, Plant; D054884:Host-Pathogen Interactions; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics; D059467:Transcriptome; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000382:microbiology
chemical_list: D010940:Plant Proteins
keywords: FHB; Fusarium graminearum; Genome-wide transcriptomics; Susceptibility response; Wheat