title: Biosensors based on modularly designed synthetic peptides for recognition, detection and live/dead differentiation of pathogenic bacteria.
abstract: Rapid and sensitive detection of bacterial pathogens is critical for assessing public health, food and environmental safety. We report the use of modularly designed and site-specifically oriented synthetic antimicrobial peptides (sAMPs) as novel recognition agents enabling detection and quantification of bacterial pathogens. The oriented assembly of the synthetic peptides on electrode surfaces through an engineered cysteine residue coupled with impedimetric detection facilitated rapid and sensitive detection of bacterial pathogens with a detection limit of 10(2)CFU/mL for four bacterial strains including Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis). The approach enabled differentiation between live and dead bacteria. The fabrication of the sAMPs functionalized surface and the importance of the sAMPs orientation for providing optimum recognition and detection ability against pathogens are discussed. The proposed methodology provides a universal platform for the detection of bacterial pathogens based on engineered peptides, as alternative to the most commonly used immunological and gene based assays. The method can also be used to fabricate antimicrobial coatings and surfaces for inactivation and screening of viable bacteria.
authors.affiliation: Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699-5810, USA.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699-5810, USA; University of Carthage, National Institute of Applied Sciences and Technology (INSAT), Laboratoire d'Ecologie et Technologie Microbienne (LETMi), 1080 Tunis, Tunisia; Tunis El Manar University, Higher Institute of Applied Biological Sciences (ISSBAT), 1006 Tunis, Tunisia. Electronic address: mouna.marrakchi@issbat.rnu.tn.
authors.forename: Mouna
authors.initials: M
authors.lastname: Marrakchi
authors.affiliation: Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699-5810, USA.
authors.forename: Dawei
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699-5810, USA. Electronic address: hdong@clarkson.edu.
authors.forename: He
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699-5810, USA. Electronic address: eandrees@clarkson.edu.
authors.forename: Silvana
authors.initials: S
authors.lastname: Andreescu
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007239:Infections / Q000175:diagnosis / Q000382:microbiology; D057230:Limit of Detection; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000472:pathogenicity; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000472:pathogenicity; D013212:Staphylococcus epidermidis / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D023181:Antimicrobial Cationic Peptides
keywords: Antimicrobial peptides; Impedimetric biosensor; Live/dead assay; Pathogenic bacteria