title: Simultaneous spectrophotometric determination of indacaterol and glycopyrronium in a newly approved pharmaceutical formulation using different signal processing techniques of ratio spectra.
abstract: Three spectrophotometric methods have been developed and validated for determination of indacaterol (IND) and glycopyrronium (GLY) in their binary mixtures and novel pharmaceutical dosage form. The proposed methods are considered to be the first methods to determine the investigated drugs simultaneously. The developed methods are based on different signal processing techniques of ratio spectra namely; Numerical Differentiation (ND), Savitsky-Golay (SG) and Fourier Transform (FT). The developed methods showed linearity over concentration range 1-30 and 10-35 (μg/mL) for IND and GLY, respectively. The accuracy calculated as percentage recoveries were in the range of 99.00%-100.49% with low value of RSD% (<1.5%) demonstrating an excellent accuracy of the proposed methods. The developed methods were proved to be specific, sensitive and precise for quality control of the investigated drugs in their pharmaceutical dosage form without the need for any separation process.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Maha F
authors.initials: MF
authors.lastname: Abdel Ghany
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Lobna A
authors.initials: LA
authors.lastname: Hussein
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Nancy
authors.initials: N
authors.lastname: Magdy
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. Electronic address: hend.z.yamani@gmail.com.
authors.forename: Hend Z
authors.initials: HZ
authors.lastname: Yamani
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000032:analysis*; D002214:Capsules; D005583:Fourier Analysis; D006024:Glycopyrrolate / Q000032:analysis*; D007189:Indans / Q000032:analysis*; D057230:Limit of Detection; D018727:Muscarinic Antagonists / Q000032:analysis*; D015363:Quinolones / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D002214:Capsules; D007189:Indans; D018727:Muscarinic Antagonists; D015363:Quinolones; C510790:indacaterol; D006024:Glycopyrrolate
keywords: Fourier Transform; Glycopyrronium; Indacaterol; Numerical Differentiation; Savitsky–Golay