title: Small quinolinium-based enzymatic probes via blue-to-red ratiometric fluorescence.
abstract: A small fluorescence ratiometric probe consisting of a single dye species, N-methyl-6-hydroxyquinolinium (MHQ), and coupled enzymatic substrates, exhibits a dramatic colour change (deep blue to red) and possesses a huge response ratio (over 2000 fold) upon specific recognition of target enzymes. Such dramatic responses are attributed to the excited-state proton transfer processes of MHQ molecules in water. Here the detection of β-galactosidase and porcine pancreatic lipase is successfully demonstrated and this class of molecules has the potential to be developed as a "naked-eye" probe in vitro.
authors.affiliation: Heifei National Laboratory for Physical Sciences at the Microscale and Department of Polymer Science and Engieering, University of Science and Technology of China, 230026 Hefei, P. R. China. gzhang@ustc.edu.cn.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: Heifei National Laboratory for Physical Sciences at the Microscale and Department of Polymer Science and Engieering, University of Science and Technology of China, 230026 Hefei, P. R. China. gzhang@ustc.edu.cn.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Du
authors.affiliation: CAS Key Laboratory of Brain Function and Disease and Department of Neurobiology and Biophysics, School of Life Sciences, University of Science and Technology of China, 230026 Hefei, P. R. China.
authors.forename: Huijing
authors.initials: H
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Brain Function and Disease and Department of Neurobiology and Biophysics, School of Life Sciences, University of Science and Technology of China, 230026 Hefei, P. R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Bi
authors.affiliation: Heifei National Laboratory for Physical Sciences at the Microscale and Department of Polymer Science and Engieering, University of Science and Technology of China, 230026 Hefei, P. R. China. gzhang@ustc.edu.cn.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D055162:Biocatalysis; D003116:Color; D006868:Hydrolysis; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D011806:Quinolinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013552:Swine; D001616:beta-Galactosidase / Q000032:analysis* / Q000378:metabolism
chemical_list: D011806:Quinolinium Compounds; D008049:Lipase; D001616:beta-Galactosidase