title: HPLC Analysis, Optimization of Extraction Conditions and Biological Evaluation of Corylopsis coreana Uyeki Flos.
abstract: A method for the separation and quantification of three flavonoids and one isocoumarin by reverse-phase high performance liquid chromatography (HPLC) has been developed and validated. Four constituents present in a crude ethanolic extract of the flowers of Coryloposis coreana Uyeki, were analyzed. Bergenin, quercetin, quercitrin and isosalipurposide were used as calibration standards. In the present study, an excellent linearity was obtained with an r² higher than 0.999. The chromatographic peaks showed good resolution. In combination with other validation data, including precision, specificity, and accuracy, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of bergenin, quercetin, quercitrin and isosalipurposide in the crude ethanolic extract of C. coreana Uyeki flos. Furthermore, the plant extracts were analyzed with HPLC to determine the four constituents and compositional differences in the extracts obtained under different extraction conditions. Several extracts of them which was dependent on the ethanol percentage of solvent were also analyzed for their antimicrobial and antioxidant activities. One hundred % ethanolic extract from C. coreana Uyeki flos showed the best antimicrobial activity against the methicillin-resistant Staphylococcus aureus (MRSA) strain. Eighty % ethanolic extract showed the best antioxidant activity and phenolic content. Taken of all, these results suggest that the flower of C. coreana Uyeki flos may be a useful source for the cure and/or prevention of septic arthritis, and the validated method was useful for the quality control of C. coreana Uyeki.
authors.affiliation: Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea. wlgpsid7156@naver.com.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Korea. wjddms1568@nate.com.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Korea. s1004jh@gmail.com.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Korea. gyoon@mokpo.ac.kr.
authors.forename: Goo
authors.initials: G
authors.lastname: Yoon
authors.affiliation: Research Develpoment Team, Jeonnam Bioindustry Foundation, Food Research Institute, Naju, Jeonnam 520-330, Korea. methyl@nate.com.
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Bang
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. csbae210@chonnam.ac.kr.
authors.forename: Chun-Sik
authors.initials: CS
authors.lastname: Bae
authors.affiliation: Department of Convergence Medicine, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea. kjlee@amc.seoul.kr.
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea. dhj1221@paran.com.
authors.forename: Dae-Hun
authors.initials: DH
authors.lastname: Park
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Korea. sscho@mokpo.ac.kr.
authors.forename: Seung-Sik
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification; D002138:Calibration; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D000431:Ethanol; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D029201:Hamamelidaceae / Q000737:chemistry*; D006801:Humans; D049934:Isocoumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012997:Solvents
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001578:Benzopyrans; D047188:Chalcones; D005419:Flavonoids; D049934:Isocoumarins; D010936:Plant Extracts; D012997:Solvents; C012526:quercitrin; D000431:Ethanol; C027321:isosalipurposide; D011794:Quercetin; C006741:bergenin
keywords: Corylopsis coreana Uyeki flos; HPLC; flavonoid; isocoumarin; simultaneous analysis