title: Paper-based fluorescence resonance energy transfer assay for directly detecting nucleic acids and proteins.
abstract: Paper-based fluorescence resonance energy transfer assay (FRET) is gaining great interest in detecting macro-biological molecule. It is difficult to achieve conveniently and fast detection for macro-biological molecule. Herein, a graphene oxide (GO)-based paper chip (glass fiber) integrated with fluorescence labeled single-stranded DNA (ssDNA) for fast, inexpensive and direct detection of biological macromolecules (proteins and nucleic acids) has been developed. In this paper, we employed the Cy3/FAM-labeled ssDNA as the reporter and the GO as quencher and the original glass fiber paper as data acquisition substrates. The chip which was designed and fabricated by a cutting machine is a miniature biosensor that monitors fluorescence recovery from resonance energy transfer. The hybridization assays and fluorescence detection were all simplified, and the surface of the chip did not require immobilization or washing. A Nikon Eclipse was employed as excited resource and a commercial digital camera was employed for capturing digital images. This paper-based microfluidics chip has been applied in the detection of proteins and nucleic acids. The biosensing capability meets many potential requirements for disease diagnosis and biological analysis.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China; Department of Chemistry and Institutes of Biomedical Sciences, Shanghai Suxin Co. Ltd., PR China. Electronic address: fxech@fudan.edu.cn.
authors.forename: Xueen
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China. Electronic address: jlkong@fudan.edu.cn.
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D006108:Graphite / Q000737:chemistry; D006801:Humans; D044085:Microfluidics; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000737:chemistry; D010209:Paper; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D045663:Quantum Dots / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D009696:Nucleic Acids; D009841:Oligonucleotides; D011506:Proteins; D006108:Graphite
keywords: FRET; Nucleic acid and protein detection; Paper-based microfluidic