title: Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats.
abstract: We investigated the effect of high phosphorus content on the sodium-phosphate cotransporter (NaPi-IIa and NaPi-IIl). Forty-eight Sprague-Dawley rats were divided into 3 groups: high-phosphorus group (HP) with fructose diphosphate sodium injection; self-manufactured low-phosphorus diet group (LP); and normal diet group (NP). At the 1st, 2nd, 4th, and 6th weeks, 4 rats from each group were sacrificed for detecting serum levels of calcium, phosphorus, and intact parathyroid hormone. Semi-quantitative retrovirus-polymerase chain reaction was used to detect the expression of NaPi-IIa and NaPi-III mRNA in kidney. At the 1st, 2nd, 4th, and 6th weeks, serum phosphorus and parathyroid hormone levels in HP group were significantly higher than those in LP and NP groups (P < 0.05). Serum calcium levels in the 3 groups showed no difference (P > 0.05). Comparing the expression of NaPi-IIa mRNA in HP group with LP and NP groups, NaPi-IIa mRNA expression was significantly reduced in HP group (P < 0.05), while NaPi-IIa mRNA expression in LP group began increasing at the 4th week (P < 0.05). At the 1st, 2nd, and 4th weeks, the expression of NaPi-III mRNA in HP, LP, and NP groups showed no clear differences (P > 0.05), while at the 6th week in HP group, NaPi-III mRNA expression was slightly increased compared to in LP and NP groups (P < 0.05). Hyperphosphatemia significantly affected NaPi-IIa and NaPi-III mRNA expression, and a factor promote an increase in intact parathyroid hormone independently of calcium.
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.|Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Department of Health Economics, School of Public Health, Harbin Medical University, Harbin, China.
authors.forename: L-M
authors.initials: LM
authors.lastname: Shi
authors.affiliation: Quality Control Office, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Q-G
authors.initials: QG
authors.lastname: Zhu
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: C-L
authors.initials: CL
authors.lastname: Jin
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: H-P
authors.initials: HP
authors.lastname: Wang
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: X-P
authors.initials: XP
authors.lastname: Hu
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: G-J
authors.initials: GJ
authors.lastname: Yuan
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Y-K
authors.initials: YK
authors.lastname: Zhao
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chou
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D005786:Gene Expression Regulation; D054559:Hyperphosphatemia / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000097:blood; D010710:Phosphates / Q000008:administration & dosage; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D050611:Sodium-Phosphate Cotransporter Proteins, Type III / Q000235:genetics* / Q000378:metabolism; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa / Q000235:genetics* / Q000378:metabolism
chemical_list: D010281:Parathyroid Hormone; D010710:Phosphates; D012333:RNA, Messenger; C493588:Slc20a1 protein, rat; C493556:Slc34a1 protein, rat; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa; D002118:Calcium