title: Genetic expression analysis of E2F-associated phosphoprotein in stress responses in the mouse.
abstract: Dysfunction of the monoaminergic system is critical in stress and anxiety disorders, but the role of each family member in the development of stress-related psychopathologies is not sufficiently understood. Eapp has been reported to be a transcriptional repressor of monoamine oxidase B (Maob) and down-regulates Maob via the Maob core promoter. In the present study, we more specifically examine the role of Eapp in stress responses by testing the hypothesis that Eapp may be involved in the occurrence and development of stress responses. Western blotting, qPCR and immunohistochemistry were used to investigate the expression variation of Eapp in hypothalamus tissue after exposure to stress. The expression of Eapp is controlled by a cis-acting quantitative trait locus (cis-eQTL). Two genes Sphk2 and Nosip, had trans-eQTLs that mapped to the location of Eapp and altered expression of these two genes was shown following siRNA knockdown of Eapp. Additionally, Mmp9, Npy, Npy5r and Maob were shown to have different expression levels in the Eapp knock-down experiments. Our data provide strong evidence that the cis-modulated gene, Eapp, is associated with stress responses, and that validated downstream targets and members of Eapp gene network may also be involved in the development of stress.
authors.affiliation: Department of Science and Education, Second People's Hospital of Nantong, Nantong University, Nantong, Jiangsu Province 226001, China; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province 226001, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Histology and Embryology, Medical College, Nantong University, Nantong, Jiangsu 226001, China; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province 226001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
authors.affiliation: Department of Neurology, Nantong University Affiliated Mental Health Center, Nantong, Jiangsu 226001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Neurology, Nantong University Affiliated Mental Health Center, Nantong, Jiangsu 226001, China.
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Histology and Embryology, Medical College, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Rixin
authors.initials: R
authors.lastname: Cai
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province 226001, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province 226001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Genetics, Genomics and Informatics, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Anatomy and Neurobiology, University of Tennessee Health Science Center, USA.
authors.forename: Kristin
authors.initials: K
authors.lastname: Hamre
authors.affiliation: Department of Histology and Embryology, Medical College, Nantong University, Nantong, Jiangsu 226001, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China; Department of Genetics, Genomics and Informatics, USA. Electronic address: lulu521uthsc@gmail.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008995:Monoamine Oxidase / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011401:Promoter Regions, Genetic; D040641:Quantitative Trait Loci; D013315:Stress, Psychological / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D000099157:Sphingosine Kinase
chemical_list: D004268:DNA-Binding Proteins; D008995:Monoamine Oxidase; D017853:Phosphotransferases (Alcohol Group Acceptor); D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; C503750:EAPP protein, mouse; C000603447:sphingosine kinase 2, mouse; D000099157:Sphingosine Kinase; C000601030:NOSIP protein, mouse
keywords: Anxiety disorder; Chronic stress; EQTL; Eapp; Hypothalamus