title: The fate of (13)C-labelled and non-labelled inulin predisposed to large bowel fermentation in rats.
abstract: The fate of stable-isotope (13)C labelled and non-labelled inulin catabolism by the gut microbiota was assessed in a healthy rat model. Sprague-Dawley male rats were randomly assigned to diets containing either cellulose or inulin, and were fed these diets for 3 days. On day (d) 4, rats allocated to the inulin diet received (13)C-labelled inulin. The rats were then fed the respective non-labelled diets (cellulose or inulin) until sampling (d4, d5, d6, d7, d10 and d11). Post feeding of (13)C-labelled substrate, breath analysis showed that (13)C-inulin cleared from the host within a period of 36 hours. Faecal (13)C demonstrated the clearance of inulin from gut with a (13)C excess reaching maximum at 24 hours (d5) and then declining gradually. There were greater variations in caecal organic acid concentrations from d4 to d6, with higher concentrations of acetic, butyric and propionic acids observed in the rats fed inulin compared to those fed cellulose. Inulin influenced caecal microbial glycosidase activity, increased colon crypt depth, and decreased the faecal output and polysaccharide content compared to the cellulose diet. In summary, the presence of inulin in the diet positively influenced large bowel microbial fermentation.
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz.
authors.forename: Christine A
authors.initials: CA
authors.lastname: Butts
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand.
authors.forename: Gunaranjan
authors.initials: G
authors.lastname: Paturi
authors.affiliation: AgResearch Grasslands, Palmerston North 4442, New Zealand.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Tavendale
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz.
authors.forename: Duncan
authors.initials: D
authors.lastname: Hedderley
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz.
authors.forename: Halina M
authors.initials: HM
authors.lastname: Stoklosinski
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz.
authors.forename: Thanuja D
authors.initials: TD
authors.lastname: Herath
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz.
authors.forename: Douglas
authors.initials: D
authors.lastname: Rosendale
authors.affiliation: AgResearch Grasslands, Palmerston North 4442, New Zealand and Riddet Institute, Massey University, Palmerston North 4442, New Zealand and Gravida: National Centre for Growth and Development, The University of Auckland, Auckland 1142, New Zealand.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Roy
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz and Riddet Institute, Massey University, Palmerston North 4442, New Zealand.
authors.forename: John A
authors.initials: JA
authors.lastname: Monro
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand. chrissie.butts@plantandfood.co.nz and Riddet Institute, Massey University, Palmerston North 4442, New Zealand.
authors.forename: Juliet
authors.initials: J
authors.lastname: Ansell
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D002432:Cecum / Q000378:metabolism* / Q000382:microbiology; D004040:Dietary Carbohydrates / Q000032:analysis / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D000069196:Gastrointestinal Microbiome; D007420:Intestine, Large / Q000378:metabolism* / Q000382:microbiology; D007444:Inulin / Q000737:chemistry / Q000378:metabolism*; D007553:Isotope Labeling; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002247:Carbon Isotopes; D004040:Dietary Carbohydrates; D007444:Inulin