title: [Role of platelet-activating factor receptor in adhesion and invasion of Aggregatibacter actinomycetemcomitans in human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To explore the role of platelet-activating factor receptor (PAFR) in adhesion and invasion of phospho- rylcholine (PC)-positive Aggregatibacter actinomycetemcomitans in cultured human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  Cultured HUVECs were pretreated with the PAFR antagonist CV3988 or anti-human PAFR monoclonal antibody for 30 min before infection with PC-positive or -negative A. actinomycetemcomitans strains. The bacterial adhesion and invasion and cytotoxicity in the cells were examined using MTT assay.
  
  
  RESULTS
  Pretreatment with PAFR antagonists at 100, 200 and 500 nmol/L significantly reduced the adhesion rate (36.29∓3.52)%, (19.04∓3.35)% and (7.69∓3.19%), respectively] and invasion rate [(12.12∓1.58)%, (7.08∓0.29)% and (2.60∓2.26)%, respectively] of PC-positive A.actinomycetemcomitans in HUVECs. Similarly, pretreatment with anti-PAFR antibody also significantly reduced A.actinomycetemcomitans adhesion and invasion in HUVECs [(50.05∓5.28)% and (39.09∓6.50)%, respectively]. Pretreatment with PAFR antagonist (200 and 500 nmol/L) and anti-PAFR antibody (25 µg/mL) significantly increased the viability of HUVECs incubated with PC-positive A.actinomycetemcomitans from (25.39∓9.33)% to (91.12∓3.14)%, (94.12∓2.15)% and (65.5∓1.87)%, respectively, but such pretreatments did not increase the viability of cells incubated with PC-negative A.actinomycetemcomitans.
  
  
  CONCLUSIONS
  PAFR plays an important role in the adhesion, invasion, and cytotoxicity of PC-positive A.actinomycetemcomitans in cultured HUVECs.
authors.affiliation: Department of Periodontology, Stomatological Hospital Affiliated to Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.E-mail: wangqin115@126.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Dongying
authors.initials: D
authors.lastname: Xuan
authors.forename: Deyu
authors.initials: D
authors.lastname: Zhong
authors.forename: Yarong
authors.initials: Y
authors.lastname: Qu
authors.forename: Jingyi
authors.initials: J
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Jincai
authors.initials: J
authors.lastname: Zhanag
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000472:pathogenicity*; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000382:microbiology*; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*
chemical_list: D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor