title: Residual expression of SMYD2 and SMYD3 is associated with the acquisition of complex karyotype in chronic lymphocytic leukemia.
abstract: SET and MYND domain containing 2 (SMYD2) and the SET and MYND domain containing 3 (SMYD3) are the most studied and well-characterized members of SMYD family. It has been demonstrated that their altered expression is associated with the progression of several solid tumors. Nevertheless, whether these methyltransferases exert any impact in chronic lymphocytic leukemia (CLL) remains unknown. Here, we investigated the gene expression profile of SMYD2 and SMYD3 in 59 samples of CLL and 10 normal B cells. The obtained results were associated with white blood cells (WBC) and platelet counts, ZAP-70 protein expression, and cytogenetic analysis. We found that SMYD2 and SMYD3 are overexpressed in CLL patients and, interestingly, patients with residual expression of both genes presented a high WBC count and complex karyotype. Furthermore, a strong correlation between SMYD2 and SMYD3 gene expression was unveiled. Our data demonstrate the association of a residual expression of SMYD2 and SMYD3 with CLL progression indicators and suggests both genes are regulated by a common transcriptional control in this type of cancer. These results may provide the basis for the development of new therapeutic strategies to prevent CLL progression.
authors.affiliation: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Av. L2 Norte, Brasília, DF, 70.910-900, Brazil.
authors.forename: Wilson
authors.initials: W
authors.lastname: Oliveira-Santos
authors.affiliation: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Av. L2 Norte, Brasília, DF, 70.910-900, Brazil.
authors.forename: Doralina Amaral
authors.initials: DA
authors.lastname: Rabello
authors.affiliation: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14.048-900, Brazil.
authors.forename: Antônio Roberto
authors.initials: AR
authors.lastname: Lucena-Araujo
authors.affiliation: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14.048-900, Brazil.
authors.forename: Fábio Morato
authors.initials: FM
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14.048-900, Brazil.
authors.forename: Eduardo Magalhaes
authors.initials: EM
authors.lastname: Rego
authors.affiliation: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Av. L2 Norte, Brasília, DF, 70.910-900, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Pittella Silva
authors.affiliation: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Av. L2 Norte, Brasília, DF, 70.910-900, Brazil. felipearaujo@unb.br.
authors.forename: Felipe
authors.initials: F
authors.lastname: Saldanha-Araujo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D011495:Histone-Lysine N-Methyltransferase; C512569:SMYD2 protein, human; C506278:SMYD3 protein, human
keywords: Chronic lymphocytic leukemia; Complex karyotype; Cytogenetic; SMYD; ZAP-70