title: Assays for Posttranslational Modifications of Intermediate Filament Proteins.
abstract: Intermediate filament (IF) proteins are known to be regulated by a number of posttranslational modifications (PTMs). Phosphorylation is the best-studied IF PTM, whereas ubiquitination, sumoylation, acetylation, glycosylation, ADP-ribosylation, farnesylation, and transamidation are less understood in functional terms but are known to regulate specific IFs under various contexts. The number and diversity of IF PTMs is certain to grow along with rapid advances in proteomic technologies. Therefore, the need for a greater understanding of the implications of PTMs to the structure, organization, and function of the IF cytoskeleton has become more apparent with the increased availability of data from global profiling studies of normal and diseased specimens. This chapter will provide information on established methods for the isolation and monitoring of IF PTMs along with the key reagents that are necessary to carry out these experiments.
authors.affiliation: Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, USA. Electronic address: ntsnider@med.unc.edu.
authors.forename: Natasha T
authors.initials: NT
authors.lastname: Snider
authors.affiliation: Department of Molecular & Integrative Physiology, Department of Medicine, University of Michigan, Ann Arbor, Michigan, USA; VA Ann Arbor Healthcare System, Ann Arbor, Michigan, USA.
authors.forename: M Bishr
authors.initials: MB
authors.lastname: Omary
mesh_terms: D000107:Acetylation; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D054875:Ubiquitination
chemical_list: D007381:Intermediate Filament Proteins
keywords: Acetylation; Cytoskeleton; Farnesylation; Glycosylation; Intermediate filaments; Keratins; Phosphorylation; Posttranslational modifications; Sumoylation; Transamidation