title: Genomic Characterization of Enterobacter cloacae Isolates from China That Coproduce KPC-3 and NDM-1 Carbapenemases.
abstract: Here, we report twoEnterobacter cloacaesequence type 231 isolates coproducing KPC-3 and NDM-1 that have caused lethal infections in a tertiary hospital in China. TheblaNDM-1-harboring plasmids carry IncA/C2and IncR replicons, showing a mosaic plasmid structure, and theblaNDM-1is harbored on a novel class I integron-like element.blaKPC-3is located on a Tn3-ΔblaTEM-1-blaKPC-3-ΔTn1722element, flanked by two 9-bp direct-repeat sequences and harbored on an IncX6 plasmid.
authors.affiliation: Department of Clinical Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA kreiswba@njms.rutgers.edu hong_du@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Du
authors.affiliation: Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Kalyan D
authors.initials: KD
authors.lastname: Chavda
authors.affiliation: Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Pandey
authors.affiliation: Department of Clinical Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Yi-Wei
authors.identifier: http://orcid.org/0000-0003-4888-6771
authors.initials: YW
authors.lastname: Tang
authors.affiliation: Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA kreiswba@njms.rutgers.edu hong_du@126.com.
authors.forename: Barry N
authors.initials: BN
authors.lastname: Kreiswirth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology; D002681:China; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology / Q000401:mortality / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D012093:Replicon; D016019:Survival Analysis; D062606:Tertiary Care Centers; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D001618:beta-Lactamases; C498060:beta-lactamase KPC-3, Enterobacter cloacae; C552864:beta-lactamase NDM-1