title: Column Selection for Biomedical Analysis Supported by Column Classification Based on Four Test Parameters.
abstract: This article focuses on correlating the column classification obtained from the method created at the Katholieke Universiteit Leuven (KUL), with the chromatographic resolution attained in biomedical separation. In the KUL system, each column is described with four parameters, which enables estimation of the FKUL value characterising similarity of those parameters to the selected reference stationary phase. Thus, a ranking list based on the FKUL value can be calculated for the chosen reference column, then correlated with the results of the column performance test. In this study, the column performance test was based on analysis of moclobemide and its two metabolites in human plasma by liquid chromatography (LC), using 18 columns. The comparative study was performed using traditional correlation of the FKUL values with the retention parameters of the analytes describing the column performance test. In order to deepen the comparative assessment of both data sets, factor analysis (FA) was also used. The obtained results indicated that the stationary phase classes, closely related according to the KUL method, yielded comparable separation for the target substances. Therefore, the column ranking system based on the FKUL-values could be considered supportive in the choice of the appropriate column for biomedical analysis.
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland. aplenis@gumed.edu.pl.
authors.forename: Alina
authors.initials: A
authors.lastname: Plenis
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland. nataliarekowska@gumed.edu.pl.
authors.forename: Natalia
authors.initials: N
authors.lastname: Rekowska
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland. tbaczek@gumed.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bączek
mesh_terms: D000928:Antidepressive Agents / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005163:Factor Analysis, Statistical; D006801:Humans; D057230:Limit of Detection; D020912:Moclobemide / Q000097:blood / Q000302:isolation & purification*
chemical_list: D000928:Antidepressive Agents; D020912:Moclobemide
keywords: Katholieke Universiteit Leuven method; column classification system; factor analysis; high-performance liquid chromatography; human plasma; moclobemide and its two metabolites