title: Evidence of hormesis on human neuronal SK-N-BE cells treated with sodium arsenate: impact at the mitochondrial level.
abstract: Exposure of human neuronal SK-N-BE cells to sodium arsenate (AsV 0.1-400 μM; 48 h) induced a biphasic toxic effect evoking hormesis. Indeed, at low concentrations, AsV stimulates cell proliferation visualized by phase contrast microscopy, whereas at high concentrations, an induction of cell death associated with a loss of cell adhesion was observed. These side effects were confirmed with crystal violet test, cell cycle analysis, evaluation of the percentage of Ki67 positive cells, and staining with propidium iodide. The impact of AsV on mitochondrial functions, which was determined by the MTT assay, the measurement of mitochondrial transmembrane potential with DiOC6(3), and the rate of mitochondrial ATP, also support an hormesis process. In addition, in the presence of high concentrations of AsV, a significant decrease of the protein expression of OXPHOS complexes of the respiratory chain was observed by western blot supporting that AsV-induced cell death is associated with mitochondrial alterations. Therefore, there are some evidences of hormesis on AsV-treated SK-N-BE cells, and at high concentrations, the mitochondria are a target of toxicity induced by AsV.
authors.affiliation: University Bourgogne Franche Comté, Université de Bourgogne Laboratoire Bio-PeroxIL, 'Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique' EA7270 / INSERM, Faculté des Sciences Gabriel, Dijon, France. wafa.kharroubi@hotmail.com.|Laboratoire de biochimie « Nutrition -aliments fonctionnels et santé vasculaire « LR- NAFS LR12ES05, Faculté de Médecine, Monastir, Tunisie. wafa.kharroubi@hotmail.com.
authors.forename: Wafa
authors.initials: W
authors.lastname: Kharroubi
authors.affiliation: University Bourgogne Franche Comté, Université de Bourgogne Laboratoire Bio-PeroxIL, 'Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique' EA7270 / INSERM, Faculté des Sciences Gabriel, Dijon, France.|Laboratoire de biochimie « Nutrition -aliments fonctionnels et santé vasculaire « LR- NAFS LR12ES05, Faculté de Médecine, Monastir, Tunisie.
authors.forename: Samia Haj
authors.initials: SH
authors.lastname: Ahmed
authors.affiliation: University Bourgogne Franche Comté, Université de Bourgogne Laboratoire Bio-PeroxIL, 'Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique' EA7270 / INSERM, Faculté des Sciences Gabriel, Dijon, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Nury
authors.affiliation: University Bourgogne Franche Comté, Université de Bourgogne Laboratoire Bio-PeroxIL, 'Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique' EA7270 / INSERM, Faculté des Sciences Gabriel, Dijon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Andreoletti
authors.affiliation: Unité de recherche de génétique « Histologie et de Cytogénétique 02/UR/ 08-03 »Faculté de Médecine de Monastir, Monastir, Tunisie.
authors.forename: Zohra
authors.initials: Z
authors.lastname: Haouas
authors.affiliation: University Bourgogne Franche Comté, Université de Bourgogne Laboratoire Bio-PeroxIL, 'Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique' EA7270 / INSERM, Faculté des Sciences Gabriel, Dijon, France.|Laboratoire de biochimie « Nutrition -aliments fonctionnels et santé vasculaire « LR- NAFS LR12ES05, Faculté de Médecine, Monastir, Tunisie.
authors.forename: Amira
authors.initials: A
authors.lastname: Zarrouk
authors.affiliation: Laboratoire de biochimie « Nutrition -aliments fonctionnels et santé vasculaire « LR- NAFS LR12ES05, Faculté de Médecine, Monastir, Tunisie.
authors.forename: Rachid
authors.initials: R
authors.lastname: Sakly
authors.affiliation: Laboratoire de biochimie « Nutrition -aliments fonctionnels et santé vasculaire « LR- NAFS LR12ES05, Faculté de Médecine, Monastir, Tunisie.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
authors.affiliation: University Bourgogne Franche Comté, Université de Bourgogne Laboratoire Bio-PeroxIL, 'Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique' EA7270 / INSERM, Faculté des Sciences Gabriel, Dijon, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Lizard
mesh_terms: D001149:Arsenates / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004579:Electron Transport; D015386:Hazardous Substances / Q000633:toxicity*; D059165:Hormesis; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*
chemical_list: D001149:Arsenates; D015386:Hazardous Substances; C009277:sodium arsenate
keywords: Human neuronal SK-NB-E; Mitochondrial dysfunctions; OXPHOS complexes; Sodium arsenate