title: Tumor-Penetrating Peptide-Modified DNA Tetrahedron for Targeting Drug Delivery.
abstract: DNA self-assembling nanostructure has been considered as a promising candidate as a drug delivery vehicle because of its compactness, mechanical stability, and noncytotoxicity. In this work, we developed functional, multiform DNA nanostructures by appending a tumor-penetrating peptide to tetrahedral DNA nanostructure (p-TDN). This functional structure is able to efficiently increase the rate of uptake of glioblastoma cell U87MG compared with the DNA tetrahedron and the double-stranded DNA structures. We found that the DNA tetrahedron plays the main role in the endocytosis of U87MG cells, whereas the tumor-penetrating peptide could also bind to transmembrane glycoprotein neuropilin-1 and mediate the endocytosis of the p-TDN nanostructure. Moreover, given the high efficiency of the growth inhibitory effect of the p-TDN loading doxorubicin hydrochloride, the p-TDN distinguishes itself as a promising candidate as an effective delivery carrier.
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Xia
authors.affiliation: National Engineering Research Center for Nanotechnology , Shanghai 200241, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , Jinan, Shandong 250031, China.
authors.forename: Xunwei
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , Shanghai 200240, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: National Engineering Research Center for Nanotechnology , Shanghai 200241, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: National Engineering Research Center for Nanotechnology , Shanghai 200241, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.affiliation: National Engineering Research Center for Nanotechnology , Shanghai 200241, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , Jinan, Shandong 250031, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , Shanghai 200240, China.|National Engineering Research Center for Nanotechnology , Shanghai 200241, China.
authors.forename: Dannong
authors.initials: D
authors.lastname: He
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D018736:DNA Adducts / Q000008:administration & dosage* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000903:Antibiotics, Antineoplastic; D018736:DNA Adducts; D010446:Peptide Fragments; C031902:doxorubicin-DNA; D004317:Doxorubicin