title: [Evaluation of the measurement of hemoglobin by the Hemocue System® in the preterm neonate less than 28 days old].
abstract: BACKGROUND
  Hemoglobin (Hb) measurement is essential for the monitoring of anemia in preterm neonates to assess if any bleeding (pulmonary, cerebral, digestive) is present. EDTA samples require 500 μL vs. 10 μL for the Hemocue(®) system. This system has been evaluated and validated in adults and children but not in preterm neonates with fetal hemoglobin. The aim of the study was to compare Hb measurement with the Hemocue(®) system vs. the EDTA laboratory system on fetal Hb in preterm neonates.
  
  
  METHODS
  This was a prospective study conducted in the preterm intensive care unit in the Amiens Hospital. Preterm neonates, before 28 days of life, requiring EDTA (Hb) measurement were included. Two Hemocues(®) were performed at the same time. Postnatal age (correlated to the fetal hemoglobin level decrease), blood sample site, and other factors that could influence the Hb result were evaluated.
  
  
  RESULTS
  Seventy-six EDTA and 152 Hemocue(®) samples from 38 preterm neonates were included. The term was 28.1±3.7 weeks of gestation, the birth weight was 1215.5±657 g. We found a good correlation between the Hemocue(®) and EDTA samples (Hemocue(®)=EDTA*0.94-0.4; R(2)=0.63; P<0.001). The influence of confounding factors was insignificant.
  
  
  CONCLUSIONS
  The use of the Hemocue(®) system showed a good correlation with the EDTA measurement of fetal Hb, with a moderate bias (-0.2±1.5 g/dL), which remained stable for the first 28 days of life.
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France; PériTox (UMI 01), faculté de médecine, université Picardie - Jules-Verne, 80000 Amiens, France.
authors.forename: L
authors.initials: L
authors.lastname: Barcat
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: C
authors.initials: C
authors.lastname: Dekens
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: E
authors.initials: E
authors.lastname: Caron-Lesenechal
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: C
authors.initials: C
authors.lastname: Degorre
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: A
authors.initials: A
authors.lastname: Cauliez
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: B
authors.initials: B
authors.lastname: Riou
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: A
authors.initials: A
authors.lastname: Vasseur
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
authors.forename: V
authors.initials: V
authors.lastname: Gromada
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France; PériTox (UMI 01), faculté de médecine, université Picardie - Jules-Verne, 80000 Amiens, France.
authors.forename: A
authors.initials: A
authors.lastname: Leke
authors.affiliation: PériTox (UMI 01), faculté de médecine, université Picardie - Jules-Verne, 80000 Amiens, France.
authors.forename: V
authors.initials: V
authors.lastname: Bach
authors.affiliation: Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France; PériTox (UMI 01), faculté de médecine, université Picardie - Jules-Verne, 80000 Amiens, France. Electronic address: tourneux.pierre@chu-amiens.fr.
authors.forename: P
authors.initials: P
authors.lastname: Tourneux
mesh_terms: D005319:Fetal Hemoglobin / Q000032:analysis; D006452:Hemoglobinometry; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D011446:Prospective Studies
chemical_list: D006454:Hemoglobins; D005319:Fetal Hemoglobin