title: miR-sc3, a Novel MicroRNA, Promotes Schwann Cell Proliferation and Migration by Targeting Astn1.
abstract: MicroRNAs (miRNAs, miRs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level. We have previously identified a group of novel miRNAs in proximal sciatic nerve after sciatic nerve transection by Solexa sequencing, and miR-sc3 is a member of the group. In this study, we aimed to investigate the effects of miR-sc3 on phenotypic modulation of Schwann cells (SCs). miR-sc3 was highly expressed in the injured nerve after sciatic nerve transection. An increased and decreased expression of miR-sc3 promoted and reduced the proliferation and migration of primary SCs, respectively. miR-sc3 directly targeted astrotactin 1 (Astn1) and led to translational suppression of Astn1. There was an inverse association between the time-dependent expressions of miR-sc3 and Astn1 in proximal sciatic nerve after sciatic nerve transection. Overall, miR-sc3 affected SC proliferation and migration by targeting Astn1, thus playing the regulatory role in peripheral nerve regeneration.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yi
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Chun
authors.initials: C
authors.lastname: Yao
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.forename: Shiying
authors.initials: S
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009416:Nerve Regeneration / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C000615641:ASTN1 protein, rat; C000615640:MIRNsc3 microRNA, rat; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface