title: The self-crosslinking smart hyaluronic acid hydrogels as injectable three-dimensional scaffolds for cells culture.
abstract: Although the disulfide bond crosslinked hyaluronic acid hydrogels have been reported by many research groups, the major researches were focused on effectively forming hydrogels. However, few researchers paid attention to the potential significance of controlling the hydrogel formation and degradation, improving biocompatibility, reducing the toxicity of exogenous and providing convenience to the clinical operations later on. In this research, the novel controllable self-crosslinking smart hydrogels with in-situ gelation property was prepared by a single component, the thiolated hyaluronic acid derivative (HA-SH), and applied as a three-dimensional scaffold to mimic native extracellular matrix (ECM) for the culture of fibroblasts cells (L929) and chondrocytes. A series of HA-SH hydrogels were prepared depending on different degrees of thiol substitution (ranging from 10 to 60%) and molecule weights of HA (0.1, 0.3 and 1.0 MDa). The gelation time, swelling property and smart degradation behavior of HA-SH hydrogel were evaluated. The results showed that the gelation and degradation time of hydrogels could be controlled by adjusting the component of HA-SH polymers. The storage modulus of HA-SH hydrogels obtained by dynamic modulus analysis (DMA) could be up to 44.6 kPa. In addition, HA-SH hydrogels were investigated as a three-dimensional scaffold for the culture of fibroblasts cells (L929) and chondrocytes cells in vitro and as an injectable hydrogel for delivering chondrocytes cells in vivo. These results illustrated that HA-SH hydrogels with controllable gelation process, intelligent degradation behavior, excellent biocompatibility and convenient operational characteristics supplied potential clinical application capacity for tissue engineering and regenerative medicine.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: bian.shaoquan@stu.scu.edu.cn.
authors.forename: Shaoquan
authors.initials: S
authors.lastname: Bian
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: 2014226070002@stu.scu.edu.cn.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: He
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: suijunhui2013@163.com.
authors.forename: Junhui
authors.initials: J
authors.lastname: Sui
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: 2014226070018@stu.scu.edu.cn.
authors.forename: Hanxu
authors.initials: H
authors.lastname: Cai
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: sunyong8702@scu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: jie_l88@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: fan_yujiang@scu.edu.cn.
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. Electronic address: zhangxd@scu.edu.cn.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000648:ultrastructure; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D020100:Hydrogels; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D013438:Sulfhydryl Compounds; D006820:Hyaluronic Acid
keywords: Controllable; Hydrogel; Self-crosslinking; Thiolated hyaluronic acid; Three-dimensional scaffold