title: A NEW SPECTROPHOTOMETRIC METHOD FOR THE ASSAY OF LISINOPRIL.
abstract: OBJECTIVE
  A new spectrophotometric method for the assay of lisinopril using 2,4-dinitrophenol as reagent is described.
  
  
  METHODS
  The method involved the addition of 2,4-dinitrophenol to lisinopril in methanol followed by absorbance measurement at 400 nm. Experimental conditions that provide wide linear range, maximum sensitivity, selectivity, accuracy and precisions were optimized.
  
  
  CONCLUSIONS
  Beer's law was obeyed in the concentration range 2.0-14.0 μg/mL. Linear regression equation of calibration graph was A = 0.005 + 0.045 x concentration, with a regression coefficient (r) of 0.9995 (n = 8). The limits of detection (LOD) and quantification (LOQ) calculated according to the ICH guidelines were 0.42 and 1.42 μg/mL, respectively. Accuracy and precision of the assays were determined by computing the intra-day and inter-day variations at three different lisinopril concentrations; the intra-day and inter-day RSD was < 1.43% and accuracy was better than 1.72%.
  
  
  CONCLUSIONS
  The proposed method is simple, easy to perform, sensitive, linear, precise, accurate and robust.
authors.forename: Mădălina
authors.initials: M
authors.lastname: Vieriu
authors.forename: Gladiola
authors.initials: G
authors.lastname: Tântaru
authors.forename: M
authors.initials: M
authors.lastname: Apostu
authors.forename: Alina Diana
authors.initials: AD
authors.lastname: Panainte
authors.forename: Nela
authors.initials: N
authors.lastname: Bibire
mesh_terms: D019297:2,4-Dinitrophenol / Q000737:chemistry; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D007202:Indicators and Reagents / Q000737:chemistry; D017706:Lisinopril / Q000737:chemistry*; D008432:Mathematical Computing; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; D017706:Lisinopril; D019297:2,4-Dinitrophenol; D000432:Methanol