title: Complete genome sequence and comparative genome analysis of the Paenibacillus mucilaginosus K02.
abstract: OBJECTIVE
  Paenibacillus mucilaginosus (P. mucilaginosus) K02 is implicated in mineral weathering. However, relevant molecular mechanisms remain obscure. The study aims to uncover the bacterium's physiological processes using genomic approaches.
  
  
  RESULTS
  Genomic DNA from P. mucilaginosus K02 was sequenced using high-throughput Solexa sequencing technology and then conducted for Clusters of Orthologous Group (COG) annotation. Thereafter, genome sequences of K02 were compared with two strains, 3016 and KNP414. Mummer was applied for collinearity analysis of three P. mucilaginosus genomes. BLAST was used to identify pan and core genes in these strains. Finally, a phylogenetic tree was constructed using the maximum likelihood method by TreeBeST. Complete genome sequence of P. mucilaginosus K02 indicated the strain comprises one circular chromosome with 8,819,200 bases containing 58.3% GC content and 84.75% coding regions. A total of 7299 predicted ORFs were identified in the genome, among them, several genes were related to carbonic anhydrase (CA), and exopolysaccharide biosynthesis and secretion. Moreover, proteins of the predicted genes were annotated in COG categories such as "Carbohydrate transport and metabolism" and "Inorganic ion transport and metabolism." In comparison with KNP414 and 3016, K02 exhibited chromosomal recombination or transposition. A total of 6662 core genes were identified among three P. mucilaginosus strains. The phylogenomic study indicated that P. mucilaginosus K02 was clustered with P. mucilaginosus strains 3016 and KNP414.
  
  
  CONCLUSIONS
  In P. mucilaginosus K02, genes related to CA and exopolysaccharide biosynthesis and secretion, and that involved in metabolism-related processes might play significant roles in mineral weathering.
authors.affiliation: Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China; Institute of Biology and Medical Sciences, Soochow University, Suzhou 215123, China. Electronic address: boxiao_suda@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Institute of Biology and Medical Sciences, Soochow University, Suzhou 215123, China. Electronic address: sunyf@suda.edu.cn.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Sun
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: bin2368@vip.163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Lian
authors.affiliation: Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China. Electronic address: ycchentm@163.com.
authors.forename: Tian-Ming
authors.initials: TM
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins
keywords: Comparative genome study; Complete genome sequence; Mineral weathering; Paenibacillus mucilaginosus K02; Phylogenomic analysis