title: 2-Aminoacetophenone Is the Main Volatile Phase I Skatole Metabolite in Pietrain × Baden-Württemberg Hybrid Type Boars.
abstract: Skatole metabolites have been considered as putative contributors to boar taint. Recently, 2-aminoacetophenone, a volatile phase I skatole metabolite, was identified in back fat samples from boars of Pietrain × Baden-Württemberg hybrid type. This paper addresses the question of the physiological origin of the observed 2-aminoacetophenone in these pigs. Microsomal fractions from nine boars were isolated, and formation of skatole metabolites was subsequently analyzed by stable-isotope dilution analysis (SIDA) using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS). Significant breed-related differences in phase I skatole metabolism were observed, explaining the high levels of 2-aminoacetophenone in Pietrain × Baden-Württemberg hybrid type boars.
authors.affiliation: Department of Nutrition and Food Sciences, Bioanalytics, University of Bonn , Endenicher Allee 11-13, D-53115 Bonn, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Gerlach
authors.affiliation: Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn , An der Immenburg 4, D-53121 Bonn, Germany.|ELFI Analytik GbR, Massenhausener Straße 18a, D-85375 Neufahrn, Germany.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Elsinghorst
authors.affiliation: Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Competence Centre for Wine Research , Breitenweg 71, D-67435 Neustadt an der Weinstraße, Germany.
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Schmarr
authors.affiliation: Department of Nutrition and Food Sciences, Bioanalytics, University of Bonn , Endenicher Allee 11-13, D-53115 Bonn, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wüst
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001947:Breeding; D005260:Female; D006824:Hybridization, Genetic; D008297:Male; D015394:Molecular Structure; D012862:Skatole / Q000737:chemistry / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D055549:Volatile Organic Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000098:Acetophenones; D055549:Volatile Organic Compounds; C055495:2-aminoacetophenone; D012862:Skatole
keywords: HS-SPME/GC-MS; SIDA; metabolism; microsomes; skatole