title: Tracking Immunological Responses of Islet Antigen-Specific T Cells in the Nonobese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
abstract: Tracking autoreactive cells in vivo is important in the study of autoimmune diseases, such as type 1 diabetes. This method provides a model to study the responses of T cells responding to physiologically relevant and organ-specific antigen. Intracellular fluorescent tracers are useful tools to identify adoptively transferred T cells. Firstly, they provide a unique fluorescent signal to distinguish adoptively transferred from endogenous cells. Secondly, cytoplasmic dyes can be used to evaluate proliferation, as the fluorescent intensity is halved with each round of cell division. This provides an important readout to assess cell activation and function.
authors.affiliation: Institute of Molecular and Experimental Medicine, Cardiff School of Medicine, Cardiff University, Tenovus Building, Heath Park, CF14 4XN, UK.
authors.forename: Terri C
authors.initials: TC
authors.lastname: Thayer
authors.affiliation: Institute of Molecular and Experimental Medicine, Cardiff School of Medicine, Cardiff University, Tenovus Building, Heath Park, CF14 4XN, UK. Wongfs@cardiff.ac.uk.
authors.forename: F Susan
authors.initials: FS
authors.lastname: Wong
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D058948:Cell Tracking / Q000379:methods*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007515:Islets of Langerhans / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic
keywords: Antigen-specific proliferation; CD8 T cell; CFDA SE; Nonobese diabetic mouse; Type 1 diabetes