title: Direct Evidence of the Link Between Energetic Metabolism and Proliferation Capacity of Cancer Cells In Vitro.
abstract: The aim of the study was to assess the link between the metabolic profile and the proliferation capacity of a range of human and murine cancer cell lines. First, the combination of mitochondrial respiration and glycolytic efficiency measurements allowed the determination of different metabolic profiles among the cell lines, ranging from a mostly oxidative to a mostly glycolytic phenotype. Second, the study revealed that cell proliferation, evaluated by DNA synthesis measurements, was statistically correlated to glycolytic efficiency. This indicated that glycolysis is the key energetic pathway linked to cell proliferation rate. Third, to validate this hypothesis and exclude non-metabolic factors, mitochondria-depleted were compared to wild-type cancer cells, and the data showed that enhanced glycolysis observed in mitochondria-depleted cells is also associated with an increase in proliferation capacity.
authors.affiliation: Biomedical Magnetic Resonance Research Group, Université catholique de Louvain (UCL), Avenue Mounier 73 (B1.73.08), 1200, Brussels, Belgium.
authors.forename: Géraldine
authors.initials: G
authors.lastname: De Preter
authors.affiliation: Biomedical Magnetic Resonance Research Group, Université catholique de Louvain (UCL), Avenue Mounier 73 (B1.73.08), 1200, Brussels, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Danhier
authors.affiliation: Institut de Recherche Expérimentale et Clinique (IREC), Pole of Pharmacology, Université catholique de Louvain (UCL), Brussels, Belgium.
authors.forename: Paolo E
authors.initials: PE
authors.lastname: Porporato
authors.affiliation: Institut de Recherche Expérimentale et Clinique (IREC), Pole of Pharmacology, Université catholique de Louvain (UCL), Brussels, Belgium.
authors.forename: Valéry L
authors.initials: VL
authors.lastname: Payen
authors.affiliation: Biomedical Magnetic Resonance Research Group, Université catholique de Louvain (UCL), Avenue Mounier 73 (B1.73.08), 1200, Brussels, Belgium.
authors.forename: Bénédicte F
authors.initials: BF
authors.lastname: Jordan
authors.affiliation: Institut de Recherche Expérimentale et Clinique (IREC), Pole of Pharmacology, Université catholique de Louvain (UCL), Brussels, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Sonveaux
authors.affiliation: Biomedical Magnetic Resonance Research Group, Université catholique de Louvain (UCL), Avenue Mounier 73 (B1.73.08), 1200, Brussels, Belgium. bernard.gallez@uclouvain.be.
authors.forename: Bernard
authors.initials: B
authors.lastname: Gallez
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004734:Energy Metabolism; D006019:Glycolysis; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D010101:Oxygen Consumption
keywords: Aerobic glycolysis; Cancer metabolism; Electron paramagnetic resonance; Oxidative phosphorylation; Proliferation