title: Prevention of Treacher Collins syndrome craniofacial anomalies in mouse models via maternal antioxidant supplementation.
abstract: Craniofacial anomalies account for approximately one-third of all birth defects and are a significant cause of infant mortality. Since the majority of the bones, cartilage and connective tissues that comprise the head and face are derived from a multipotent migratory progenitor cell population called the neural crest, craniofacial disorders are typically attributed to defects in neural crest cell development. Treacher Collins syndrome (TCS) is a disorder of craniofacial development and although TCS arises primarily through autosomal dominant mutations in TCOF1, no clear genotype-phenotype correlation has been documented. Here we show that Tcof1 haploinsufficiency results in oxidative stress-induced DNA damage and neuroepithelial cell death. Consistent with this discovery, maternal treatment with antioxidants minimizes cell death in the neuroepithelium and substantially ameliorates or prevents the pathogenesis of craniofacial anomalies in Tcof1(+/-) mice. Thus maternal antioxidant dietary supplementation may provide an avenue for protection against the pathogenesis of TCS and similar neurocristopathies.
authors.affiliation: Organization for Research Initiatives and Development, Doshisha University, Karasuma Higashi-iru, Imadegawa-dori, Kamigyo, Kyoto 602-8580, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakai
authors.affiliation: Dental School, Faculty of Medical and Human Sciences, Manchester Academic Health Sciences Centre, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Jill
authors.initials: J
authors.lastname: Dixon
authors.affiliation: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, USA.
authors.forename: Annita
authors.initials: A
authors.lastname: Achilleos
authors.affiliation: Dental School, Faculty of Medical and Human Sciences, Manchester Academic Health Sciences Centre, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK.|Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Dixon
authors.affiliation: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, USA.|Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Trainor
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D019587:Dietary Supplements / Q000032:analysis*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008342:Mandibulofacial Dysostosis / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D039382:Maternal Nutritional Physiological Phenomena; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy
chemical_list: D000975:Antioxidants; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C491352:Tcof1 protein, mouse