title: Lentivirus-mediated Persephin over-expression in Parkinson's disease rats.
abstract: Persephin, together with glial cell line-derived neurotrophic factor and neurturin, has a neurotrophic effect and promotes the survival of motor neurons cultured in vitro. In this study, dopaminergic neurons in the substantia nigra of rats were transfected with the Persephin gene. One week later 6-hydroxydopamine was injected into the anterior medial bundle to establish a Parkinson's disease model in the rats. Results found that the number of dopaminergic neurons in the substantia nigra increased, tyrosine hydroxylase expression was upregulated and concentrations of dopamine and its metabolites in corpus striatum were increased after pretreatment with Persephin gene. In addition, the rotating effect of the induced Parkinson's disease rats was much less in the group pretreated with the Persephin gene. Persephin has a neuroprotective effect on the 6-hydroxydopamine-induced Parkinson's disease through protecting dopaminergic neurons.
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Yin
authors.affiliation: Department of Physiology, Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Hua-Min
authors.initials: HM
authors.lastname: Xu
authors.affiliation: Nursing College of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Jiang
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Yun-Lai
authors.initials: YL
authors.lastname: Zhi
authors.affiliation: Department of Nursing, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Yu-Xiu
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Heng-Wei
authors.initials: HW
authors.lastname: Xiang
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Xiao-Dong
authors.identifier: 0000-0002-7872-0919
authors.initials: XD
authors.lastname: Ding
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Peng
authors.identifier: 0000-0001-5202-3819
authors.initials: P
authors.lastname: Sun
keywords: Parkinson's disease; Persephin; dopaminergic neurons; gene therapy; lentivirus; nerve regeneration; neural regeneration; over-expression; striatum; transfection