title: Effect of pasteurisation on the concentrations of vitamin D compounds in donor breastmilk.
abstract: Premature infants are the main recipients of pasteurised donor human milk (PDHM), when their mothers are unable to provide their own. In this study, we evaluated the effect of pasteurisation on the concentrations of vitamin D compounds in donor breastmilk. Milk samples were obtained pre- and post-Holder pasteurisation. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyse the samples for vitamins D2 and D3 (D2 and D3) and 25-hydroxyvitamins D2 and D3 (25(OH)D2 and 25(OH)D3). The significance of differences in vitamin D concentrations between the two groups of milk samples was assessed using the Wilcoxon matched-pairs signed rank test, in which p < 0.05 was considered significant. Pasteurisation resulted in a significant reduction (p < 0.05) in the content of D2, D3, 25(OH)D2 and 25(OH)D3. The losses ranged from 10% to 20% following pasteurisation.
authors.affiliation: a School of Pharmacy , the University of Queensland , Brisbane , Queensland , Australia ;
authors.forename: Fabio P
authors.initials: FP
authors.lastname: Gomes
authors.affiliation: a School of Pharmacy , the University of Queensland , Brisbane , Queensland , Australia ;
authors.forename: P Nicholas
authors.initials: PN
authors.lastname: Shaw
authors.affiliation: a School of Pharmacy , the University of Queensland , Brisbane , Queensland , Australia ;|b Royal Brisbane and Women's Hospital , Metro North Health Services District , Herston , Queensland , Australia ;
authors.forename: Karen
authors.initials: K
authors.lastname: Whitfield
authors.affiliation: b Royal Brisbane and Women's Hospital , Metro North Health Services District , Herston , Queensland , Australia ;|c Department of Paediatrics & Child Health , the University of Queensland , Brisbane , Queensland , Australia.
authors.forename: Pieter
authors.initials: P
authors.lastname: Koorts
authors.affiliation: b Royal Brisbane and Women's Hospital , Metro North Health Services District , Herston , Queensland , Australia ;
authors.forename: Helen
authors.initials: H
authors.lastname: McConachy
authors.affiliation: a School of Pharmacy , the University of Queensland , Brisbane , Queensland , Australia ;
authors.forename: Amitha K
authors.initials: AK
authors.lastname: Hewavitharana
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000032:analysis; D002112:Calcifediol / Q000032:analysis; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D061352:Pasteurization; D053719:Tandem Mass Spectrometry; D014807:Vitamin D / Q000032:analysis*; D014815:Vitamins / Q000032:analysis
chemical_list: D014815:Vitamins; D014807:Vitamin D; D015652:25-Hydroxyvitamin D 2; D002112:Calcifediol
keywords: Bone disorders; Holder pasteurisation; Milk bank; preterm infants