title: Electromembrane extraction of gonadotropin-releasing hormone agonists from plasma and wastewater samples.
abstract: In the present study, for the first time electromembrane extraction followed by high performance liquid chromatography coupled with ultraviolet detection was optimized and validated for quantification of four gonadotropin-releasing hormone agonist anticancer peptides (alarelin, leuprolide, buserelin and triptorelin) in biological and aqueous samples. The parameters influencing electromigration were investigated and optimized. The membrane consists 95% of 1-octanol and 5% di-(2-ethylhexyl)-phosphate immobilized in the pores of a hollow fiber. A 20 V electrical field was applied to make the analytes migrate from sample solution with pH 7.0, through the supported liquid membrane into an acidic acceptor solution with pH 1.0 which was located inside the lumen of hollow fiber. Extraction recoveries in the range of 49 and 71% within 15 min extraction time were obtained in different biological matrices which resulted in preconcentration factors in the range of 82-118 and satisfactory repeatability (7.1 < RSD% < 19.8). The method offers good linearity (2.0-1000 ng/mL) with estimation of regression coefficient higher than 0.998. The procedure allows very low detection and quantitation limits of 0.2 and 0.6 ng/mL, respectively. Finally, it was applied to determination and quantification of peptides in human plasma and wastewater samples and satisfactory results were yielded.
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Nojavan
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: Tina
authors.initials: T
authors.lastname: Bidarmanesh
authors.affiliation: Pharmaceutical Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.|Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mohammadi
authors.affiliation: Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Yaripour
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D006801:Humans; D016014:Linear Models; D008567:Membranes, Artificial; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D007987:Gonadotropin-Releasing Hormone; D008567:Membranes, Artificial; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Anticancer peptides; Electromembrane extraction; Gonadotropin-releasing hormone; Plasma