title: [Therapeutic effect of GDNF gene-modified mesencephalic neural stem cell transplantation in a rat model of Parkinson disease].
abstract: OBJECTIVE
  To evaluate the therapeutic effect of transplantation of mesencephalic neural stem cells (mNSCs) genetically modified by glial cell line-derived neurotrophic factor (GDNF) gene in a rat model of Parkinson disease.
  
  
  METHODS
  mNSCs isolated from the lateral component of the midbrain of fetal rats at gestational age of 14 or 15 days were cultured for 5 days before genetic modification with GFP or GDNF gene. Rat models of Parkinson disease established by stereotactic injection of 6-hydroxy dopamine in the ventral area of the midbrain and the medial forebrain bundle were randomized into 3 groups to receive PBS injection, GFP gene-modified mNSCs transplantation, or GDNF gene-modified mNSCs transplantation into the right stratum. The behavioral changes of the rats were evaluated by observing rotations induced by intraperitoneal injection of apomorphine after the transplantation, and the survival, migration and differentiation of the transplanted cells were identified by immunohistochemistry.
  
  
  RESULTS
  Transplantation with GDNF gene-modified mNSCs significantly improved the behavioral abnormalities of the rat models as compared with PBS injection and GFP gene-modified mNSCs transplantation. At 56 days after the transplantation, a greater number of the transplanted cells survived in the rat brain and more differentiated dopaminergic neurons were detected in GDNF gene-modified mNSCs transplantation group than in GFP gene-modified mNSCs transplantation group.
  
  
  CONCLUSIONS
  GDNF gene-modified mNSCs transplantation can significantly improve dyskinesia in rat models of Parkinson disease, but the molecular mechanism needs further clarification.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.E-mail: dkjremark@126.com.
authors.forename: Kuijia
authors.initials: K
authors.lastname: Duan
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Mingguo
authors.initials: M
authors.lastname: Wang
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xingli
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics* / Q000627:therapeutic use; D008636:Mesencephalon / Q000166:cytology; D058953:Neural Stem Cells / Q000637:transplantation*; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D033581:Stem Cell Transplantation
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor