title: Vesicular monoamine transporter 2 (Vmat2) knockdown elicits anxiety-like behavior in zebrafish.
abstract: Vesicular monoamine transporter 2 (Vmat2) is widely distributed in the central nervous system, and responsible for uptaking transmitters into the vesicles. However, whether Vmat2-deficiency is related to the anxiety is rarely investigated, especially in zebrafish. Here, we reported Vmat2 heterzygous mutant zebrafish displayed anxiety-like behavior. The mutants spent less time in the top area and took longer latency to the top in the novel tank test. Consistently, they showed dark avoidance in the light/dark box test, with longer duration in the light zone and increased number of crossing between the two zones. Monoamine concentration analysis showed that the levels of monoamine neurotransmitters including dopamine (DA), 5-hydroxy tryptamine (5-HT) and norepinephrine (NE), as well as their metabolites were decreased in VMAT mutants. Taken together, these findings suggest that Vmat2 heterzygous mutant zebrafish may serve as a new model of anxiety, which may be related with the low level of DA, 5-HT and NE.
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Siyue
authors.initials: S
authors.lastname: Li
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Hu
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Zhao-Min
authors.initials: ZM
authors.lastname: Zhong
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Institutes of Neuroscience, Soochow University, Suzhou 215123, China; Beijing Key Laboratory for Parkinson's Disease, Beijing 100053, China. Electronic address: liuchunfeng@suda.edu.cn.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Liu
mesh_terms: D000818:Animals; D001007:Anxiety / Q000503:physiopathology*; D001362:Avoidance Learning; D001522:Behavior, Animal; D004195:Disease Models, Animal; D055785:Gene Knockdown Techniques; D050493:Vesicular Monoamine Transport Proteins / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D050493:Vesicular Monoamine Transport Proteins
keywords: Anxiety; Vesicular monoamine transporter 2; Zebrafish