title: Cefotaxime-resistant Escherichia coli in broiler farms-A cross-sectional investigation in Germany.
abstract: In this investigation the farm prevalence of cefotaxime-resistant Escherichia coli (CREC) in German broiler farms was evaluated. In total, 59 flocks on 34 broiler farms were sampled in four agricultural regions of Germany. Per broiler flock, three faecal samples, a pair of boot swabs and one dust sample were taken and examined for the presence of CREC. After pre-enrichment of sample material in Luria-Bertani-broth, the broth was streaked onto MacConkey agar containing 1mg/l cefotaxime (CTX). CREC isolates were detected in at least one sample from each flock resulting in a farm prevalence of 100%. The proportion of positive samples was high in all three sample types. Of 177 collective faecal samples 81.9% were positive, of 59 boot swabs and 59 dust samples 79.7% and 62.7% were positive. In conclusion, the prevalence of broiler farms with cefotaxime-resistant E. coli in Germany is very high. We suggest that the analysis of collective faecal samples is sufficient to determine the CREC farm status. In addition to other studies our study supports the finding that cefotaxime resistance is a good proxy for the presence of ESBL- or plasmidic AmpC-beta-lactamases.
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Hering
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Frömke
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: von Münchhausen
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Hartmann
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Schneider
authors.affiliation: Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Free University Berlin, Robert-von-Ostertag-Str. 7-13, D-14163 Berlin, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Friese
authors.affiliation: Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Free University Berlin, Robert-von-Ostertag-Str. 7-13, D-14163 Berlin, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Rösler
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Kreienbrock
authors.affiliation: Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany. Electronic address: katja.hille@tiho-hannover.de.
authors.forename: Katja
authors.initials: K
authors.lastname: Hille
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology*; D002645:Chickens; D003430:Cross-Sectional Studies; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005858:Germany / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology; D015995:Prevalence; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D002439:Cefotaxime
keywords: Antibiotic resistance; ESBL; Epidemiological study; Poultry