title: [Cabozantinib inhibits Listeria monocytogenes infection in mice].
abstract: OBJECTIVE
  To test the effect of the c-Met inhibitor cabozantinib in inhibiting infections by Listeria monocytogenes (LM) in mice.
  
  
  METHODS
  C57BL/6 mice at 6 weeks of age were subjected to intraperitoneal injection of LM and randomized into 4 groups for treatment with intraperitoneal injection of PBS, intragastric administration of cabozantinib (20 µg/g), intraperitoneal injection of ampicillin (Amp, 20 µg/g), or cabozantinib plus Amp. The survival curves were drawn for each group, and the number of bacteria in the blood and brain tissues was determined; serum IL-10 level and NF-κB p65 level in the cerebrospinal fluid (CSF) were assayed, and Evans Blue (EB) content and pathological changes in brain were examined.
  
  
  RESULTS
  Compared with PBS-treated mice, the mice treated with cabozantinib showed a significantly higher survival rate, lower bacterial counts in the blood and brain (P<0.05 or 0.001), lower IL-10 (P<0.05) and NF-κB p65 levels (P<0.01), lower brain EB content (P<0.001), and milder pathological changes in the brain. The blood and brain bacterial counts (P<0.001), IL-10 (P<0.01) and NF-κB p65 levels (P<0.001), and brain EB content (P<0.001) were all significantly lower in mice treated with the combination of drugs than in mice treated with cabozantinib alone.
  
  
  CONCLUSIONS
  Cabozantinib can inhibit LM infection in mice and has important values in developing new anti-intracellular infection drug.
authors.affiliation: Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.E-mail: xiaoqing081@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiao
authors.forename: Xiaolong
authors.initials: X
authors.lastname: He
authors.forename: Wenbing
authors.initials: W
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Min
authors.initials: M
authors.lastname: Long
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
mesh_terms: D000813:Anilides / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000382:microbiology / Q000473:pathology; D007274:Injections, Intraperitoneal; D016753:Interleukin-10 / Q000097:blood; D008089:Listeria monocytogenes / Q000187:drug effects*; D008088:Listeriosis / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D011725:Pyridines / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000134:cerebrospinal fluid
chemical_list: D000813:Anilides; C556062:IL10 protein, mouse; D011725:Pyridines; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D016753:Interleukin-10; C558660:cabozantinib