title: Purification and Structural Analysis of SUN and KASH Domain Proteins.
abstract: Molecular tethers span the nuclear envelope to mechanically connect the cytoskeleton and nucleoskeleton. These bridge-like tethers, termed linkers of nucleoskeleton and cytoskeleton (LINC) complexes, consist of SUN proteins at the inner nuclear membrane and KASH proteins at the outer nuclear membrane. LINC complexes are central to a variety of cell activities including nuclear positioning and mechanotransduction, and LINC-related abnormalities are associated with a spectrum of tissue-specific diseases, termed laminopathies or envelopathies. Protocols used to study the biochemical and structural characteristics of core elements of SUN-KASH complexes are described here to facilitate further studies in this new field of cell biology.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: F
authors.initials: F
authors.lastname: Esra Demircioglu
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Victor E
authors.initials: VE
authors.lastname: Cruz
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Electronic address: tus@mit.edu.
authors.forename: Thomas U
authors.initials: TU
authors.lastname: Schwartz
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D017434:Protein Structure, Tertiary
chemical_list: D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C575643:KASH5 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; C413025:SUN2 protein, human
keywords: KASH peptide; LINC complex; Nesprin; Nuclear envelope; SUN domain; SUN protein