title: Elicitors' influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions.
abstract: Cobalt nitrate, nickel sulphate, hydrogen peroxide, sodium nitroprusside, and culture filtrates of Pseudomonas monteili, Bacillus circularans, Trichoderma atroviridae, and Trichoderma harzianum were tested to elicit ginsenoside production in a cell suspension line of Panax quinquefolius. Abiotic elicitors preferentially increased panaxadiols whereas biotic elicitors upregulated the panaxatriol synthesis. Cobalt nitrate (50 μM) increased total ginsenosides content by twofold (54.3 mg/L) within 5 days. It also induced the Rc synthesis that was absent in the control cultures. Elicitation with P. monteili (2.5 % v/v, 5 days) also supported 2.4-fold enhancement in saponin yield. Elicitation by T. atroviridae or hydrogen peroxide induced the synthesis of Rg3 and Rh2 that are absent in ginseng roots. The highest ginsenosides productivity (3.2-fold of control) was noticed in cells exposed to 1.25 % v/v dose of T. atroviridae for 5 days. Treating cells with T. harzianum for 15 days afforded maximum synthesis and leaching (8.1 mg/L) of ginsenoside Rh1.
authors.affiliation: Plant Biotechnology Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India.
authors.forename: Tanya
authors.initials: T
authors.lastname: Biswas
authors.affiliation: Microbiology and Entomology Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Kalra
authors.affiliation: Plant Biotechnology Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Mathur
authors.affiliation: Genetics and Plant Breeding Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Lal
authors.affiliation: Analytical Chemistry Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Singh
authors.affiliation: Plant Biotechnology Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India. archnacimap@gmail.com.
authors.forename: Archana
authors.initials: A
authors.lastname: Mathur
mesh_terms: D001407:Bacillus / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D003470:Culture Media; D036145:Ginsenosides / Q000096:biosynthesis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D009599:Nitroprusside / Q000737:chemistry; D005894:Panax / Q000737:chemistry* / Q000166:cytology; D059828:Plant Cells / Q000187:drug effects* / Q000378:metabolism; D011549:Pseudomonas / Q000737:chemistry; D014242:Trichoderma / Q000737:chemistry
chemical_list: D003470:Culture Media; D036145:Ginsenosides; D009599:Nitroprusside; C004564:panaxadiol; C097367:ginsenoside Rg3; D003035:Cobalt; C425564:ginsenoside Rh1; C025913:cobaltous nitrate; D009532:Nickel; C004563:panaxatriol; D006861:Hydrogen Peroxide; C017558:nickel sulfide
keywords: Cell suspension; Elicitation; Exudation; Ginsenoside; Panax quinquefolius