title: Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection.
abstract: Venom of the ant spider Lachesana tarabaevi contains a wide variety of antimicrobial peptides. Among them, a special place belongs to cyto-insectotoxins, a class of cytolytic molecules showing equally potent antimicrobial and insecticidal effects. We tested one of them, CIT 1a, for ability to suppress Chlamydia trachomatis infection. HEK293 cells were transfected with plasmid vectors harboring the cit 1a gene. Controlled expression of the transgene led to a significant decrease in C. trachomatis viability inside the infected cells. Using proteomic and transcriptomic approaches, we found alterations in protein expression patterns and identified differentially expressed genes in transfected cells.
authors.affiliation: Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia.
authors.forename: Nadezhda F
authors.initials: NF
authors.lastname: Polina
authors.affiliation: Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia.
authors.forename: Marina M
authors.initials: MM
authors.lastname: Shkarupeta
authors.affiliation: Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Popenko
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya st., 117997, Moscow, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Vassilevski
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya st., 117997, Moscow, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Kozlov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya st., 117997, Moscow, Russia.
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Grishin
authors.affiliation: Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia. lazar0@mail.ru.|National Research Centre "Kurchatov Institute", 1 Akademika Kurchatova sq., 123182, Moscow, Russia. lazar0@mail.ru.
authors.forename: Vassili N
authors.initials: VN
authors.lastname: Lazarev
authors.affiliation: Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya st., 117997, Moscow, Russia.|National Research Centre "Kurchatov Institute", 1 Akademika Kurchatova sq., 123182, Moscow, Russia.
authors.forename: Vadim M
authors.initials: VM
authors.lastname: Govorun
keywords: Antimicrobial peptide; Cytolytic peptide; Gene therapy; Infectious disease; Proteomics; Transcriptome