title: Maggot debridement therapy promotes diabetic foot wound healing by up-regulating endothelial cell activity.
abstract: To determine the role of maggot debridement therapy (MDT) on diabetic foot wound healing, we compared growth related factors in wounds before and after treatment. Furthermore, we utilized human umbilical vein endothelial cells (HUVECs) to explore responses to maggot excretions/secretions on markers of angiogenesis and proliferation. The results showed that there was neo-granulation and angiogenesis in diabetic foot wounds after MDT. Moreover, significant elevation in CD34 and CD68 levels was also observed in treated wounds. In vitro, ES increased HUVEC proliferation, improved tube formation, and increased expression of vascular endothelial growth factor receptor 2 in a dose dependent manner. These results demonstrate that MDT and maggot ES can promote diabetic foot wound healing by up-regulating endothelial cell activity.
authors.affiliation: Department of Endocrinology, The 454th Hospital of PLA, Nanjing, 210002, PR China.
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, The 454th Hospital of PLA, Nanjing, 210002, PR China.
authors.forename: Jin'an
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, The 454th Hospital of PLA, Nanjing, 210002, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, The 454th Hospital of PLA, Nanjing, 210002, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, The 454th Hospital of PLA, Nanjing, 210002, PR China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, The 454th Hospital of PLA, Nanjing, 210002, PR China. Electronic address: 27966509@qq.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D003646:Debridement / Q000379:methods*; D017719:Diabetic Foot / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007814:Larva / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned
keywords: Angiogenesis; Diabetic foot ulcers; HUVEC; Maggot debridement therapy; Wound healing