title: Increased S-nitrosothiols are associated with spinal cord injury in multiple sclerosis.
abstract: Multiple sclerosis (MS) is an immune-mediated disorder associated with inflammation, demyelination and axonal damage. In search of potential biomarkers of spinal cord lesions in MS related to nitric oxide metabolites, we measured total nitrite and nitrate levels, and protein-bound nitrotyrosine and S-nitrosothiol concentrations in the serum of MS patients at different stages of the disease. Sixty-eight patients and 36 healthy volunteers were included in the study. Total nitrite and nitrate levels were augmented in relapsing-remitting MS, while increased S-nitrosothiol concentrations were found both in relapsing-remitting and secondary-progressive MS. Further analysis demonstrated that S-nitrosothiol levels were selectively increased in patients with spinal cord injury. The data suggest that high S-nitrosothiol concentration may be a potential serum biomarker for spinal cord injury in MS.
authors.affiliation: Institute of Higher Nervous Activity & Neurophysiology RAS, Department of Functional Biochemistry of the Nervous System, Butlerov Street 5A, Moscow 117485, Russia. Electronic address: hydrohinon@mail.ru.
authors.forename: Vera
authors.initials: V
authors.lastname: Fominykh
authors.affiliation: Institute of Higher Nervous Activity & Neurophysiology RAS, Department of Functional Biochemistry of the Nervous System, Butlerov Street 5A, Moscow 117485, Russia.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Onufriev
authors.affiliation: Research Center of Neurology Russian Academy of Medical Sciences, Neuroinfection Department, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Vorobyeva
authors.affiliation: Institute of Higher Nervous Activity & Neurophysiology RAS, Department of Functional Biochemistry of the Nervous System, Butlerov Street 5A, Moscow 117485, Russia.
authors.forename: Lev
authors.initials: L
authors.lastname: Brylev
authors.affiliation: Institute of Higher Nervous Activity & Neurophysiology RAS, Department of Functional Biochemistry of the Nervous System, Butlerov Street 5A, Moscow 117485, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Yakovlev
authors.affiliation: Research Center of Neurology Russian Academy of Medical Sciences, Neuroinfection Department, Moscow, Russia.
authors.forename: Maria N
authors.initials: MN
authors.lastname: Zakharova
authors.affiliation: Institute of Higher Nervous Activity & Neurophysiology RAS, Department of Functional Biochemistry of the Nervous System, Butlerov Street 5A, Moscow 117485, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Gulyaeva
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000150:complications; D026403:S-Nitrosothiols / Q000097:blood*; D013119:Spinal Cord Injuries / Q000097:blood* / Q000209:etiology
chemical_list: D015415:Biomarkers; D026403:S-Nitrosothiols
keywords: Multiple sclerosis; Nitric oxide; S-nitrosothiols; Spinal cord