title: Development of Splice Variant-Specific Monoclonal Antibodies Against Human α3 Integrin.
abstract: Integrins are a major family of adhesion molecules, consisting of heterodimers (α and β subunits). Several reports have suggested the presence of splice variants in the cytoplasmic domain of certain integrin subunits. In the present study, we detected mRNA of integrin α3 splice variants (α3A and α3B) by RT-PCR using total RNA from the human brain as a template. The α3B variant lacks the sequence coded by exon 25 and appears to be generated by alternative splicing. We established mouse hybridomas producing monoclonal antibodies (both of which are of IgG1 class) specific for each variant. Each antibody exhibited specific reactivity towards the corresponding integrin α3 variant in Western blotting and immunoprecipitation experiments, suggesting it to be a useful tool for detection of the respective integrin variant.
authors.affiliation: Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences , Tokyo, Japan .
authors.forename: Teruaki
authors.initials: T
authors.lastname: Oku
authors.affiliation: Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences , Tokyo, Japan .
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ando
authors.affiliation: Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences , Tokyo, Japan .
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ogura
authors.affiliation: Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences , Tokyo, Japan .
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D006801:Humans; D006825:Hybridomas; D039422:Integrin alpha3 / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C000599264:ITGA3 protein, human; D039422:Integrin alpha3; D021122:Protein Subunits