title: Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides.
abstract: We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. All patients had peripheral blood flow cytometry as part of their first visit. TNMB and histopathological staging of the cases were retrospectively performed in accordance with International Society for Cutaneous Lymphomas/European Organization of Research and Treatment of Cancer (ISCL/EORTC) criteria at the time of flow cytometry sampling. To determine prognostic value of cell surface antigens, cases were divided into two groups as stable and progressive disease. 17 flow cytometric analyses of 17 parapsoriasis (PP) and 11 analyses of 11 benign erythrodermic patients were included as control groups. Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3(+), CD4(+), CD8(+), TCRαβ(+), TCRγδ(+), CD7(+), CD4(+)CD7(+), CD4(+)CD7(-), and CD71(+)), B cells (HLA-DR(+), CD19(+), and HLA-DR(+)CD19(+)), NKT cells (CD3(+)CD16(+)CD56(+)), and NK cells (CD3(-)CD16(+)CD56(+)). The mean value of all cell surface antigens was not statistically significant between parapsoriasis and MF groups. Along with an increase in cases of MF stage statistically significant difference was found between the mean values of cell surface antigens. Flow cytometric analysis of peripheral blood cell surface antigens in patients with mycosis fungoides may contribute to predicting disease stage and progression.
authors.affiliation: Uludag University School of Medicine, Department of Dermatology and Venereology, Bursa, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Yazıcı
authors.affiliation: Uludag University School of Medicine, Department of Dermatology and Venereology, Bursa, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Bülbül Başkan
authors.affiliation: Uludag University School of Medicine, Department of Immunology, Bursa, Turkey.
authors.forename: Ferah
authors.initials: F
authors.lastname: Budak
authors.affiliation: Uludag University School of Medicine, Department of Immunology, Bursa, Turkey.
authors.forename: Barbaros
authors.initials: B
authors.lastname: Oral
authors.affiliation: Uludag University School of Medicine, Department of Pathology, Bursa, Turkey.
authors.forename: Şaduman Balaban
authors.initials: ŞB
authors.lastname: Adim
authors.affiliation: Uludag University School of Medicine, Department of Dermatology and Venereology, Bursa, Turkey.
authors.forename: Zübeyde
authors.initials: Z
authors.lastname: Ceylan Kalin
authors.affiliation: Uludag University School of Medicine, Department of Biostatistics, Bursa, Turkey.
authors.forename: Güven
authors.initials: G
authors.lastname: Özkaya
authors.affiliation: Uludag University School of Medicine, Department of Dermatology and Venereology, Bursa, Turkey.
authors.forename: Kenan
authors.initials: K
authors.lastname: Aydoğan
authors.affiliation: Uludag University School of Medicine, Department of Dermatology and Venereology, Bursa, Turkey.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Saricaoğlu
authors.affiliation: Uludag University School of Medicine, Department of Dermatology and Venereology, Bursa, Turkey.
authors.forename: Şükran
authors.initials: Ş
authors.lastname: Tunali
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000175:diagnosis* / Q000276:immunology; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell