title: Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues.
abstract: Keratin filaments are a hallmark of epithelial differentiation. Their cell type-specific spatial organization and dynamic properties reflect and support epithelial function. To study this interdependency, imaging of fluorescently tagged keratins is a widely used method by which the temporospatial organization and behavior of the keratin intermediate filament network can be analyzed in living cells. Here, we describe methods that have been adapted and optimized to dissect and quantify keratin intermediate filament network dynamics in vital cultured cells and functional tissues.
authors.affiliation: Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Schwarz
authors.affiliation: Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany.
authors.forename: Marcin
authors.initials: M
authors.lastname: Moch
authors.affiliation: Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Windoffer
authors.affiliation: Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany. Electronic address: rleube@ukaachen.de.
authors.forename: Rudolf E
authors.initials: RE
authors.lastname: Leube
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal
chemical_list: D007633:Keratins
keywords: Blastocyst; Bulk flow analysis; Confocal microscopy; Cytokeratin; Fluorescence recovery after photobleaching; Fluorescent protein; Live cell imaging; Motion analysis; Preimplantation embryo; Transgenic mouse