title: Nonclassical human leukocyte antigen (HLA-G, HLA-E, and HLA-F) in coronary artery disease.
abstract: OBJECTIVE
  Several evidences suggest the association between the evolution of coronary artery disease (CAD) and the development of coronary syndrome that is often associated with disrupted plaque and partial or complete thrombosis of the related artery. Because of the inflammatory nature of CAD, we investigated the human leukocyte antigen (HLA)-G, HLA-E, and HLA-F genetic polymorphisms within CAD patients and evaluated their potential association with this disease in Tunisian population.
  
  
  METHODS
  Different polymorphisms in HLA-G (14-bp Insertion/Deletion, +3142C/G), HLA-E (HLA-E*01:01/01:03 A/G), HLA-F (HLA-F*01:02 T/C, 01:03 C/T, 01:04 A/C) genes were typed using different laboratory techniques in a cohort of 89 CAD patients and 84 controls.
  
  
  RESULTS
  A significant association was reported between the HLA-G +3142 G allele (OR=1.64, 95% CI=1.05-2.56, p=0.02) and increased risk of CAD. No association was found for the other studied polymorphisms. When we considered the haplotypes, we found TDELCA and TDELGG haplotypes associated to CAD with p=0.008 and p=0.030, respectively, suggesting the potential interaction between HLA-G and HLA-E genes.
  
  
  CONCLUSIONS
  Our findings indicated that the HLA-G +3142C/G polymorphism and TDELCA and TDELGG haplotypes can harbour a reliable diagnosis value for the risk of CAD development suggesting that HLA-G, -E and -F molecules might be involved in the pathogenesis of the disease. However, further studies are necessary to confirm our results.
authors.affiliation: Laboratory Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia. Electronic address: ines.zidi@techemail.com.
authors.forename: Ines
authors.initials: I
authors.lastname: Zidi
authors.affiliation: Centre de Biotechnologie de Sfax, Molecular and Cellular Screening Process, route Sidi Mansour, BP1177, 3018 Sfax, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Kharrat
authors.affiliation: Centre de Biotechnologie de Sfax, Molecular and Cellular Screening Process, route Sidi Mansour, BP1177, 3018 Sfax, Tunisia.
authors.forename: Rania
authors.initials: R
authors.lastname: Abdelhedi
authors.affiliation: Laboratory Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Amna Ben
authors.initials: AB
authors.lastname: Hassine
authors.affiliation: Laboratory Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Ahmed Baligh
authors.initials: AB
authors.lastname: Laaribi
authors.affiliation: Laboratory Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Hamza Ben
authors.initials: HB
authors.lastname: Yahia
authors.affiliation: Faculté de Médecine de Sfax, Laboratory of Histology, Avenue Majida Boulila, 3029 Sfax, Tunisia.
authors.forename: Nouha Bouayed
authors.initials: NB
authors.lastname: Abdelmoula
authors.affiliation: Hospital of Hedi Chaker (CHU), Service de cardiologie, Route el Ain Km 0.5, 3000 Sfax, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Abid
authors.affiliation: Centre de Biotechnologie de Sfax, Molecular and Cellular Screening Process, route Sidi Mansour, BP1177, 3018 Sfax, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Rebai
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Rizzo
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D015897:Comorbidity; D003324:Coronary Artery Disease / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D059951:HLA-G Antigens / Q000235:genetics*; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014416:Tunisia; D000097625:HLA-E Antigens
chemical_list: D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens; C062341:HLA-F antigens
keywords: Coronary artery disease; HLA-E; HLA-F; HLA-G; Polymorphism