title: Association of G/T(rs222859) polymorphism in Exon 1 of YBX2 gene with azoospermia, among Iranian infertile males.
abstract: Animal model studies have shown that MSY2 gene has a potential role in spermatogenesis. Some mutations on this gene have been proposed to be associated with human male infertility. In this study, polymorphisms of exon 1 of YBX2 gene have been investigated. A total of 276 men were evaluated. They included 96 men with normal spermatogenesis, 60 men with nonobstructive azoospermia, 60 men with oligospermia and 60 men with asthenospermia. We extracted DNA from blood and testis tissues of samples, and analysed polymorphisms of exon 1 by sequencing method. Moreover, YBX2 gene expression was studied by real-time PCR on blood and testis tissue of samples. Sequencing results showed that among the studied polymorphisms, frequency of TT genotype in rs222859 polymorphism was significantly higher in azoospermic patients compared to control group (P < 0.001). Azoospermic men exhibited significant underexpression of YBX2 gene in blood and testis samples in comparison with controls, oligosperm and asthenosperm samples (P < 0.001), but there was no significant difference in gene expression of YBX2 gene in blood and testis tissues of azoospermic men, with and without mutation (P > 0.05). According to our results, the alterations of this gene might be involved in azoospermia among Iranian population.
authors.affiliation: Cellular and Molecular Research Center of Qazvin University of Medical Science, Qazvin, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Najafipour
authors.affiliation: Cellular and Molecular Research Center of Qazvin University of Medical Science, Qazvin, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Rashvand
authors.affiliation: Cellular and Molecular Research Center of Qazvin University of Medical Science, Qazvin, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Alizadeh
authors.affiliation: Fertility and Infertility Center of Shariati Hospital, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Aleyasin
authors.affiliation: Cellular and Molecular Research Center of Qazvin University of Medical Science, Qazvin, Iran. smoghbelinejad@qums.ac.ir.
authors.forename: S
authors.initials: S
authors.lastname: Moghbelinejad
mesh_terms: D053627:Asthenozoospermia / Q000235:genetics; D053713:Azoospermia / Q000235:genetics*; D016022:Case-Control Studies; D005091:Exons; D015870:Gene Expression; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D007492:Iran; D008297:Male; D009845:Oligospermia / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D016601:RNA-Binding Proteins / Q000235:genetics*
chemical_list: D016601:RNA-Binding Proteins; C485481:YBX2 protein, human
keywords: Azoospermia; YBX2 gene; male infertility