title: Translocation of Rigid Rod-Shaped Virus through Various Solid-State Nanopores.
abstract: Nanopores have been used as a high throughput tool for characterizing individual biomolecules and nanoparticles. Here, we present the translocation of rigid rod-shaped tobacco mosaic virus (TMV) through solid-state nanopores. Interestingly, due to the high rigidity of TMV, three types of events with distinctive characteristics at the capture process and a strong current fluctuation during the translocation of TMV are observed. A kinetic model is then proposed to address the dynamics of the translocation, followed by corresponding dynamics simulations. The results reveal that TMV has to rotate to fit and pass the pore when it is captured by a nanopore with an angle larger than the maximum angle that allows it to pass through. Then, we investigate the dependence of the rotation of TMV on the conductance fluctuations at the blockade stage. The results show that the rotation of TMV during the passage through the pore affects the current signal significantly. This study gives a fundamental understanding of the dynamics of rod-shaped particles translocating through the nanopore and how the current responds to it. It opens a new possible way to characterize the rigidity of analytes by nanopores.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University , Nanjing, Jiangsu 210096, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry and Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Third Affiliated Hospital of Southern Medical University , Guangzhou, Guangdong 510000, China.
authors.forename: Qizhao
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University , Nanjing, Jiangsu 210096, China.
authors.forename: Rongliang
authors.initials: R
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry and Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Baicheng
authors.initials: B
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University , Tianjin, 300071, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Ma
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry and Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Kaifu
authors.initials: K
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University , Nanjing, Jiangsu 210096, China.
authors.forename: Quanjun
authors.initials: Q
authors.lastname: Liu
mesh_terms: D058608:Nanopores; D010316:Particle Size; D013499:Surface Properties; D014027:Tobacco Mosaic Virus / Q000737:chemistry* / Q000302:isolation & purification*