title: The biosensor toolbox for plant developmental biology.
abstract: Plant development is highly interconnected with the metabolic state of tissues and cells. Current research efforts focus on the identification of the links and mechanisms that govern the interplay between metabolic and gene-regulatory networks. Genetically encoded sensors that allow detection of small molecules in vivo and at high spatio-temporal resolution promise to be the tools of choice for quantifying and visualizing the dynamics of metabolite flux in plants. We provide an overview about current approaches to measure signaling molecules, such as hormones, calcium and sugars, as well as for monitoring the metabolic state via energy equivalents and pH. Biosensors show great potential to address questions of plant development but there are also limitations where alternative approaches are needed.
authors.affiliation: Ruprecht-Karls-Universität Heidelberg, Department of Plant Molecular Biology, Centre for Organismal Studies, Im Neuenheimer Feld 360, 69120 Heidelberg, Germany.
authors.forename: Veli V
authors.initials: VV
authors.lastname: Uslu
authors.affiliation: Ruprecht-Karls-Universität Heidelberg, CellNetworks Excellence Cluster and Centre for Organismal Studies, Im Neuenheimer Feld 329, 69120 Heidelberg, Germany. Electronic address: guido.grossmann@cos.uni-heidelberg.de.
authors.forename: Guido
authors.initials: G
authors.lastname: Grossmann
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004734:Energy Metabolism; D063245:Plant Development; D010944:Plants / Q000378:metabolism*