title: In Vivo Measurement of Cerebral Mitochondrial Metabolism Using Broadband Near Infrared Spectroscopy Following Neonatal Stroke.
abstract: Neonatal stroke presents with features of encephalopathy and can result in significant morbidity and mortality. We investigated the cerebral metabolic and haemodynamic changes following neonatal stroke in a term infant at 24 h of life. Changes in oxidation state of cytochrome-c-oxidase (oxCCO) concentration were monitored along with changes in oxy- and deoxy- haemoglobin using a new broadband near-infrared spectroscopy (NIRS) system. Repeated transient changes in cerebral haemodynamics and metabolism were noted over a 3-h study period with decrease in oxyhaemoglobin (HbO2), deoxy haemoglobin (HHb) and oxCCO in both cerebral hemispheres without significant changes in systemic observations. A clear asymmetry was noted in the degree of change between the two cerebral hemispheres. Changes in cerebral oxygenation (measured as HbDiff=HbO2-HHb) and cerebral metabolism (measured as oxCCO) were highly coupled on the injured side of the brain.
authors.affiliation: Institute for Women's Health, University College London and Neonatal Unit, University College London Hospitals Trust, London, UK. subhabratamitra@hotmail.com.
authors.forename: Subhabrata
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Biomedical Optics Research Laboratory, Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
authors.forename: Gemma
authors.initials: G
authors.lastname: Bale
authors.affiliation: Institute for Women's Health, University College London and Neonatal Unit, University College London Hospitals Trust, London, UK.
authors.forename: Judith
authors.initials: J
authors.lastname: Meek
authors.affiliation: Institute for Women's Health, University College London and Neonatal Unit, University College London Hospitals Trust, London, UK.
authors.forename: Sean
authors.initials: S
authors.lastname: Mathieson
authors.affiliation: Institute for Women's Health, University College London and Neonatal Unit, University College London Hospitals Trust, London, UK.
authors.forename: Cristina
authors.initials: C
authors.lastname: Uria
authors.affiliation: Institute for Women's Health, University College London and Neonatal Unit, University College London Hospitals Trust, London, UK.
authors.forename: Giles
authors.initials: G
authors.lastname: Kendall
authors.affiliation: Institute for Women's Health, University College London and Neonatal Unit, University College London Hospitals Trust, London, UK.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Robertson
authors.affiliation: Biomedical Optics Research Laboratory, Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
authors.forename: Ilias
authors.initials: I
authors.lastname: Tachtsidis
mesh_terms: D001921:Brain / Q000378:metabolism*; D004569:Electroencephalography; D006801:Humans; D007231:Infant, Newborn; D008928:Mitochondria / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D020521:Stroke / Q000378:metabolism*
chemical_list: D010100:Oxygen
keywords: Broadband NIRS; Cerebral hemodynamics; Cytochrome c oxidase; Mitochondrial metabolism; Neonatal stroke