title: Activation of Antioxidative Functions by Radon Inhalation Enhances the Mitigation Effects of Pregabalin on Chronic Constriction Injury-Induced Neuropathic Pain in Mice.
abstract: Radon inhalation brings pain relief for chronic constriction injury- (CCI-) induced neuropathic pain in mice due to the activation of antioxidative functions, which is different from the mechanism of the pregabalin effect. In this study, we assessed whether a combination of radon inhalation and pregabalin administration is more effective against neuropathic pain than radon or pregabalin only. Mice were treated with inhaled radon at a concentration of 1,000 Bq/m(3) for 24 hours and pregabalin administration after CCI surgery. In mice treated with pregabalin at a dose of 3 mg/kg weight, the 50% paw withdrawal threshold of mice treated with pregabalin or radon and pregabalin was significantly increased, suggesting pain relief. The therapeutic effects of radon inhalation or the combined effects of radon and pregabalin (3 mg/kg weight) were almost equivalent to treatment with pregabalin at a dose of 1.4 mg/kg weight or 4.1 mg/kg weight, respectively. Radon inhalation and the combination of radon and pregabalin increased antioxidant associated substances in the paw. The antioxidant substances increased much more in radon inhalation than in pregabalin administration. These findings suggested that the activation of antioxidative functions by radon inhalation enhances the pain relief of pregabalin and that this combined effect is probably an additive effect.
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Takahiro
authors.identifier: 0000-0003-0686-0741
authors.initials: T
authors.lastname: Kataoka
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Horie
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Reo
authors.initials: R
authors.lastname: Etani
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Norie
authors.initials: N
authors.lastname: Kanzaki
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sasaoka
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kobashi
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Hanamoto
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Yamaoka
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D003250:Constriction; D008297:Male; D008813:Mice, Inbred ICR; D009437:Neuralgia / Q000188:drug therapy* / Q000473:pathology; D000069583:Pregabalin / Q000008:administration & dosage / Q000627:therapeutic use*; D011886:Radon / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D000069583:Pregabalin; D011886:Radon