title: D-repeat in the XIST gene is required for X chromosome inactivation.
abstract: XIST is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of XIST, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of XIST, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of XIST expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.
authors.affiliation: a Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun , 130062 , China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: a Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun , 130062 , China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: a Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun , 130062 , China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Song
authors.affiliation: a Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun , 130062 , China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sui
authors.affiliation: a Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun , 130062 , China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: a Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University , Changchun , 130062 , China.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
mesh_terms: D064113:CRISPR-Cas Systems; D002454:Cell Differentiation / Q000235:genetics*; D041322:Chromosomes, Human, Y; D018507:Gene Expression Regulation, Developmental; D050172:Genes, X-Linked; D057809:HEK293 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D062085:RNA, Long Noncoding / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D049951:X Chromosome Inactivation / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA
keywords: CRISPR/Cas9; D-repeat; XIST; X-inactivation; long non-coding RNA