title: Association of DNA Methylation at CPT1A Locus with Metabolic Syndrome in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) Study.
abstract: In this study, we conducted an epigenome-wide association study of metabolic syndrome (MetS) among 846 participants of European descent in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN). DNA was isolated from CD4+ T cells and methylation at ~470,000 cytosine-phosphate-guanine dinucleotide (CpG) pairs was assayed using the Illumina Infinium HumanMethylation450 BeadChip. We modeled the percentage methylation at individual CpGs as a function of MetS using linear mixed models. A Bonferroni-corrected P-value of 1.1 x 10(-7) was considered significant. Methylation at two CpG sites in CPT1A on chromosome 11 was significantly associated with MetS (P for cg00574958 = 2.6x10(-14) and P for cg17058475 = 1.2x10(-9)). Significant associations were replicated in both European and African ancestry participants of the Bogalusa Heart Study. Our findings suggest that methylation in CPT1A is a promising epigenetic marker for MetS risk which could become useful as a treatment target in the future.
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Mithun
authors.initials: M
authors.lastname: Das
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Jin
authors.initials: J
authors.lastname: Sha
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Bertha
authors.initials: B
authors.lastname: Hidalgo
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Stella
authors.initials: S
authors.lastname: Aslibekyan
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Anh N
authors.initials: AN
authors.lastname: Do
authors.affiliation: Department of Biostatistics, Section on Statistical Genetics, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Degui
authors.initials: D
authors.lastname: Zhi
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, United States of America.
authors.forename: Dianjianyi
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, United States of America.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, United States of America.
authors.forename: Shengxu
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, United States of America.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, United States of America.
authors.forename: Sathanur R
authors.initials: SR
authors.lastname: Srinivasan
authors.affiliation: Department of Biostatistics, Section on Statistical Genetics, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Hemant K
authors.initials: HK
authors.lastname: Tiwari
authors.affiliation: HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States of America.
authors.forename: Devin
authors.initials: D
authors.lastname: Absher
authors.affiliation: Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, United States of America.|IMDEA Food, Madrid, Spain.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, United States of America.
authors.forename: Gerald S
authors.initials: GS
authors.lastname: Berenson
authors.affiliation: Dean's Office, College of Public Health, University of Kentucky, Lexington, KY, United States of America.
authors.forename: Donna K
authors.initials: DK
authors.lastname: Arnett
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States of America.
authors.forename: Marguerite R
authors.initials: MR
authors.lastname: Irvin
mesh_terms: D000368:Aged; D000886:Anthropometry; D044383:Black People / Q000235:genetics; D001794:Blood Pressure; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics* / Q000502:physiology; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D015331:Cohort Studies; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000208:ethnology / Q000235:genetics*; D008875:Middle Aged; D008910:Minnesota / Q000453:epidemiology; D020411:Oligonucleotide Array Sequence Analysis; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood; D014589:Utah / Q000453:epidemiology; D044465:White People / Q000235:genetics; D001741:Black or African American
chemical_list: D014280:Triglycerides; C577474:CPT1A protein, human; D002334:Carnitine O-Palmitoyltransferase