title: QTL analysis of modifiers for pigmentary disorder in rats carrying Ednrb sl mutations.
abstract: Pigmentary variation in animals has been studied because of its application in genetics, evolution, and developmental biology. The large number of known color loci provides rich resource to elucidate the functional pigmentary system. Nonetheless, more color loci remain to be identified. In our previous study, we revealed that two different strains, namely, AGH rats and LEH rats, but which had the same null mutation of the Ednrb gene (Ednrb(sl)) showed markedly different pigmented coat ratio. This result strongly suggested that the severity of pigment abnormality was modified by genetic factor(s) in each strain. To elucidate the modifier locus of pigment disorder, we carried out whole-genome scanning for quantitative trait loci (QTLs) on 149 F2 (AGH-Ednrb(sl) × LEH-Ednrb(sl)) rats. A highly significant QTL, constituting 26% of the total pigmentation phenotype variance, was identified in a region around D7Got23 on chromosome (Chr) 7. In addition, investigation on epistatic interaction revealed significant interactions between D7Got23 and D3Rat78 and between D7Got23 and D14Mit4. Results suggested that a modified locus on Chr 7 was mainly responsible for the variance of pigmentary disorder between AGH-Ednrb(sl) rats and LEH-Ednrb(sl) rats, and two modifier loci showing epistatic interaction may, in part, influence pigment phenotype.
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.|College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Jieping
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Dang
authors.affiliation: Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.|Division of Microbiology and Genetics, Center for Animal Resources and Development, Kumamoto, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Torigoe
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.affiliation: Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors.forename: Jinxi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Agui
mesh_terms: D000483:Alleles; D000818:Animals; D000831:Animals, Newborn; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D003433:Crosses, Genetic; D004843:Epistasis, Genetic; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D010858:Pigmentation / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D051381:Rats; D044023:Receptor, Endothelin B / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D044023:Receptor, Endothelin B