title: Determination of 2-Aminoacetophenone in wine by high-performance thin-layer chromatography-fluorescence detection.
abstract: 2-Aminoacetophenone (AAP) is closely correlated with the appearance of the sensory phenomenon of UTA ("untypical aging off-flavor") in wine. AAP analyses are generally performed by gas chromatography and mass selective detection (GC/MS), when AAP is extracted from wines by liquid-liquid, solid-liquid or solid phase microextraction. Here we present a rapid, selective and sensitive method for the determination of AAP in wine by high-performance thin-layer chromatography with fluorescence detection (HPTLC-FLD). As internal standard, 2-amino-4-methoxyacetophenone was used. Liquid-liquid extraction with t-butyl methyl ether was followed by a basic cleanup of the extracts, which were applied onto HPTLC amino plates developed with methylene chloride/toluene (7+3, v/v) as mobile phase. Dipping the dried plate into hexane-paraffin solution enhanced fluorescence that was scanned at 366/>400nm. Limits of detection and quantitation were determined to be 0.1 and 0.3μgL(-1) wine, respectively, while only AAP concentrations >0.5μgL(-1) result in UTA. Recoveries were near 100% for model, white, rosé and red wines. Thus, the HPTLC-FLD method enables the analysis of AAP in wines clearly below the odor thresholds and represents a rapid and convenient screening alternative to existing GC/MS methods.
authors.affiliation: University of Hohenheim, Institute of Food Chemistry, Garbenstr. 28, 70599 Stuttgart, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Horlacher
authors.affiliation: University of Hohenheim, Institute of Food Chemistry, Garbenstr. 28, 70599 Stuttgart, Germany. Electronic address: wolfgang.schwack@uni-hohenheim.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schwack
mesh_terms: D000098:Acetophenones / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D059625:Liquid-Liquid Extraction; D009812:Odorants / Q000032:analysis; D052617:Solid Phase Microextraction; D014920:Wine / Q000032:analysis*
chemical_list: D000098:Acetophenones; C055495:2-aminoacetophenone
keywords: 2-Aminoacetophenone; Fluorescence detection; HPTLC; UTA; Untypical aging off-flavor; Wine