title: β-Cyclodextrin preventing protein aggregation in foam fractionation of bovine serum albumin.
abstract: In this work, β-cyclodextrin was developed to prevent protein aggregation in foam fractionation using bovine serum albumin (BSA) as a model protein. The role of β-cyclodextrin in preventing the aggregation of BSA induced by the gas-liquid interface was studied at the molecular level. The results indicate that by holding the exposed phenylalanine residues in its hydrophobic cavity, β-cyclodextrin effectively prevented the aggregation of BSA induced by the gas-liquid interface in foam fractionation. Furthermore, β-cyclodextrin could be effectively separated from BSA in the foamate due to their weak association.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, No. 8 Guangrong Road, Dingzi Gu, Hongqiao District, Tianjin 300130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, No. 8 Guangrong Road, Dingzi Gu, Hongqiao District, Tianjin 300130, China. Electronic address: zhaoliangwu_hebut@163.com.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Lab of Green Chemical Technology & High Efficient Energy Saving, Hebei University of Technology, No. 8 Guangrong Road, Dingzi Gu, Hongqiao District, Tianjin 300130, China. Electronic address: yjwang@hebut.edu.cn.
authors.forename: Yanji
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, No. 8 Guangrong Road, Dingzi Gu, Hongqiao District, Tianjin 300130, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Ding
authors.affiliation: Lianyungang TCM Branch of Jiangsu Union Technical Institute, Jiangsu, Lianyungang 222006, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D005591:Chemical Fractionation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel / Q000379:methods; D010649:Phenylalanine / Q000737:chemistry; D066329:Protein Aggregates; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000187:drug effects*; D013057:Spectrum Analysis / Q000379:methods; D014443:Tyrosine / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D066329:Protein Aggregates; D047392:beta-Cyclodextrins; D012710:Serum Albumin, Bovine; D014443:Tyrosine; D010649:Phenylalanine
keywords: BSA; Foam fractionation; Protein aggregation; β-Cyclodextrin