title: Prednisolone but not selenium and rtPA reduces edema and improves angiogenesis after burn in mice.
abstract: OBJECTIVE
  Despite dramatic improvements in burn care, the major part of the therapy of thermal injuries remains symptomatical in nature. A targeted approach to accelerate angiogenesis and woundhealing and reduce edema formation remains to be found. We therefore aimed to investigate the impact of anti-inflammatory, anti-coagulative and thrombolytic agents on microcirculation after thermal injuries on the mentioned parameters.
  
  
  METHODS
  Full thickness burns were inflicted on the ears of hairless mice (n=48). The effects of five intraperitoneal injections of either recombinant tissue plasminogen activator (rtPA), selenium, prednisolone or sodium chloride on microcirculation, edema formation, leukocytes and angiogenesis were investigated over a 13 day period using intravital fluorescent microscopy.
  
  
  RESULTS
  Prednisolone slightly improved angiogenesis (100.0% day 0 vs. 91.4% non-perfused area on day 1 post burn, p<0.05) and reduced edema formation (93.3% vs. 123.1% control on day 3, p<0.05). The rtPA-group showed the highest number of sticking leukocytes up to day 7 post burn (233%, 265%, 254% on days 1, 3, and 7, p<0.05 compared to baseline). A post-traumatic expansion of the non perfused area could only be observed in the selenium group (100.0% day 0, 103.1% day 1 post burn). In addition, selenium caused an increase of rolling leukocytes over the complete observation time.
  
  
  CONCLUSIONS
  The often described positive influences of selenium for the treatment of burn patients could not be confirmed, on the contrary we found a post-traumatic expansion of the non perfused area and an increase of leukocytes in this group. The expectations to rtPA did not fulfill. Prednisolone improved angiogenesis and reduced the edema formation, both Parameters are essential for wound healing and survival of burned patients.
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany; Department of Plastic, Reconstructive and Hand Surgery, Martin-Luther-Hospital, Berlin, Germany. Electronic address: ole.goertz@rub.de.
authors.forename: O
authors.initials: O
authors.lastname: Goertz
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Over
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany; Department of Plastic, Reconstructive and Hand Surgery, Martin-Luther-Hospital, Berlin, Germany.
authors.forename: L
authors.initials: L
authors.lastname: von der Lohe
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lauer
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ring
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Daigeler
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lehnhardt
authors.affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kolbenschlag
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002056:Burns / Q000000981:diagnostic imaging / Q000473:pathology*; D004423:Ear; D004487:Edema / Q000000981:diagnostic imaging / Q000473:pathology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D000069416:Intravital Microscopy; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008833:Microcirculation / Q000187:drug effects; D008856:Microscopy, Fluorescence; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011239:Prednisolone / Q000494:pharmacology*; D011994:Recombinant Proteins; D012643:Selenium / Q000494:pharmacology*; D012867:Skin / Q000000981:diagnostic imaging / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005343:Fibrinolytic Agents; D005938:Glucocorticoids; D011994:Recombinant Proteins; D011239:Prednisolone; D010959:Tissue Plasminogen Activator; D012643:Selenium
keywords: Angiogenesis; Burns; Intravital fluorescent microscopy; Leukocytes; Prednisolone; Recombinant tissue plasminogen activator; Selenium; rtPA