title: Potential role of heat-shock proteins in giant cell tumors.
abstract: We investigated the differential expression protein profile of giant cell tumors (GCTs), which can be used to monitor the tumor's recurrence and metastasis, to provide preliminary results for further study. We also explored heat-shock protein (HSP) inhibitor that prevents tumors from recurring and migrating. A stable isotope-labeling strategy using isobaric tags for relative and absolute quantitation coupled with two-dimensional liquid chromatography tandem mass spectrometry was used to separate and identify differentially expressed proteins. A total of 467 differentially expressed proteins were identified in GCT tissues. Up to 311 proteins were upregulated, whereas 156 proteins were downregulated in GCT tissues. Three of the differentially expressed HSPs, namely HP90A, HSPB1, and HSPB2, were upregulated. The differentially expressed proteins of GCT tissues will provide a scientific foundation for tumor prognosis, and for further studies exploring HSP inhibitor to prevent tumor recurrence and migration.
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of XinJiang Medical University, Urumqi, China.
authors.forename: J T
authors.initials: JT
authors.lastname: Chen
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of XinJiang Medical University, Urumqi, China.
authors.forename: A
authors.initials: A
authors.lastname: Younusi
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of XinJiang Medical University, Urumqi, China.
authors.forename: L
authors.initials: L
authors.lastname: Cao
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of XinJiang Medical University, Urumqi, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of XinJiang Medical University, Urumqi, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of XinJiang Medical University, Urumqi, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Song
mesh_terms: D002853:Chromatography, Liquid; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D018212:Giant Cell Tumor of Bone / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D007553:Isotope Labeling; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D055551:HSP27 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; C057725:HSPB1 protein, human; C442250:HSPB2 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A