title: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions.
abstract: Isothermal titration calorimetry (ITC) is a technique that measures the heat released or absorbed during a chemical reaction as an intrinsic probe to characterize any chemical process that involves heat changes spontaneously occurring during the reaction. The general features of this method to determine the kinetic and thermodynamic parameters of enzymatic reactions (kcat, KM, ΔH) are described and discussed here together with some detailed applications to specific cases. ITC does not require any modification or labeling of the system under analysis, can be performed in solution, and needs only small amounts of enzyme. These properties make ITC an invaluable, powerful, and unique tool to extend the knowledge of enzyme kinetics to drug discovery.
authors.affiliation: Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Mazzei
authors.affiliation: Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. Electronic address: stefano.ciurli@unibo.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Ciurli
authors.affiliation: Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zambelli
mesh_terms: D002151:Calorimetry / Q000379:methods*; D002384:Catalysis; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D014510:Urease / Q000378:metabolism
chemical_list: D007770:L-Lactate Dehydrogenase; D014510:Urease
keywords: Enzymatic catalysis; Enzymatic inhibition; Isothermal titration calorimetry; Lactate dehydrogenase; Urease