title: Metabolomic study of corticosterone-induced cytotoxicity in PC12 cells by ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry.
abstract: Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and investigate its potential mechanisms involved. However, up to now, the mechanism of corticosterone-induced cytotoxicity in PC12 cells was still looking forward to further elucidation. In this work, the metabolomic study of the biochemical changes caused by corticosterone-induced cytotoxicity in differentiated PC12 cells with different corticosterone concentrations was performed for the first time, using the ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS). Partial least squares-discriminate analysis (PLS-DA) indicated that metabolic profiles of different corticosterone treatment groups deviated from the control group. A total of fifteen metabolites were characterized as potential biomarkers involved in corticosterone-induced cytotoxicity, which were corresponding to the dysfunctions of five pathways including glycerophospholipid metabolism, sphingolipid metabolism, oxidation of fatty acids, glycerolipid metabolism and sterol lipid metabolism. This study indicated that the rapid and holistic cell metabolomics approach might be a powerful tool to further study the pathogenesis mechanism of corticosterone-induced cytotoxicity in PC12 cells.
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Hongye
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Medical Scientific Research Center, Guangxi Medical University, Nanning 530021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, The Maternal & Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning 530003, China.
authors.forename: Gan
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Chaoling
authors.initials: C
authors.lastname: Tang
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Shiyin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Bang
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Jinbin
authors.initials: J
authors.lastname: Wei
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Junxiang
authors.initials: J
authors.lastname: Ruan
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning 530021, China. suzhiheng915@126.com gxsonghui@163.com.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Su
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000633:toxicity*; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D013058:Mass Spectrometry / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D016716:PC12 Cells; D051381:Rats
chemical_list: D015415:Biomarkers; D003345:Corticosterone