title: Streptomyces polygonatisp. nov., an endophytic actinomycete isolated from a root ofPolygonatum odoratum(Mill.).
abstract: A novel actinomycete, designated strain NEAU-G9T, was isolated from the root ofPolygonatum odoratum(Mill.) collected from Harbin, Heilongjiang province, north China, and was characterized using a polyphasic approach. Key morphological and chemotaxonomic properties confirmed the affiliation of strain NEAU-G9Tto the genusStreptomyces.Strain NEAU-G9Tcontained ll-diaminopimelic acid as the diamino acid. The predominant menaquinones were MK-9(H8), MK-9(H6) and MK-9(H4). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant fatty acids were iso-C16 : 0, anteiso-C15 : 0and C16 : 0.16S rRNA gene sequence similarity studies showed that strain NEAU-G9Tbelongs to the genusStreptomycesand exhibits the highest sequence similarity toStreptomyces yanglinensisJCM 13275T(97.75 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-G9Tis most closely related toStreptomyces misakiensisJCM 4062T(97.12 % sequence similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-G9Tcan be clearly distinguished fromS. yanglinensisJCM 13275TandS. misakiensisJCM 4062T. Consequently, strain NEAU-G9Trepresents a novel species of the genusStreptomyces, for which the nameStreptomyces polygonatisp. nov. is proposed. The type strain is NEAU-G9T( = CGMCC 4.7237T= DSM 100521T).
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Guo
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Shuanghe
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Guan
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Feiyu
authors.initials: F
authors.lastname: Jia
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang