title: Extended Release of an Anti-Heparan Sulfate Peptide From a Contact Lens Suppresses Corneal Herpes Simplex Virus-1 Infection.
abstract: OBJECTIVE
  To prolong the release of a heparan sulfate binding peptide, G2-C, using a commercially available contact lens as a delivery vehicle and to demonstrate the ability of the released peptide to block herpes simplex virus-1 (HSV-1) infection using in vitro, ex vivo, and in vivo models of corneal HSV-1 infection.
  
  
  METHODS
  Commercially available contact lenses were immersed in peptide solution for 5 days prior to determining the release of the peptide at various time points. Cytotoxicity of the released samples was determined by MTT and cell cycle analysis, and the functional activity of the released samples were assessed by viral entry, and viral spread assay using human corneal epithelial cells (HCE). The ability to suppress infection in human and pig cornea ex vivo and mouse in vivo models were also assessed.
  
  
  RESULTS
  Peptide G2-C was released through the contact lens. Following release for 3 days, the peptide showed significant activity by inhibiting HSV-1 viral entry and spread in HCE cells. Significant suppression of infection was also observed in the ex vivo and in vivo experiments involving corneas.
  
  
  CONCLUSIONS
  Extended release of an anti-HS peptide through a commercially available contact lens can generate significant anti-HSV-1 activity and provides a new and effective way to control corneal herpes.
authors.affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States 2Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States 3Department of Pathology, University of.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Jaishankar
authors.affiliation: Department of Biopharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois, United States.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Buhrman
authors.affiliation: Department of Pathology, University of Illinois at Chicago, Chicago, Illinois, United States.
authors.forename: Tibor
authors.initials: T
authors.lastname: Valyi-Nagy
authors.affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States 2Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States 4Department of Biopharmaceutical Scienc.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Gemeinhart
authors.affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States 2Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States 5Department of Microbiology and Immunol.
authors.forename: Deepak
authors.initials: D
authors.lastname: Shukla
mesh_terms: D000818:Animals; D003261:Contact Lenses; D003315:Cornea / Q000378:metabolism / Q000821:virology*; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D015828:Eye Infections, Viral / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D005260:Female; D006497:Heparan Sulfate / Q000494:pharmacology*; D018259:Herpesvirus 1, Human; D006801:Humans; D016849:Keratitis, Herpetic / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine
chemical_list: D003692:Delayed-Action Preparations; D006497:Heparan Sulfate