title: Decreased microRNA-206 and its function in cervical cancer.
abstract: OBJECTIVE
  MicroRNA-206 plays important roles in tumorigenesis and tumor progression of various human malignancies. However, its involvement in cervical cancer has remained unclear.
  
  
  OBJECTIVE
  The aim of this study was to examine the expression patterns and clinical implications of miR-206 in cervical cancer.
  
  
  METHODS
  Quantitative RT-PCR was performed to evaluate the expression levels of miR-206 in cervical cancer cell lines and primary tumor tissues. The clinicopathologic significance and the prognostic value of miR-206 expression were further determined. Finally, the effects of miR-206 on HeLa cell proliferation, apoptosis, invasion, and migration were investigated.
  
  
  RESULTS
  MiR-206 expression was significantly downregulated in cervical cancer samples when compared with normal adjacent tissues. Low level of miR-206 was associated with advanced FIGO stage (p < 0.001), positive lymph node metastasis (p < 0.001), poor differentiation (p = 0.016), and human papillomavirus infection (p = 0.007). Multivariate Cox regression analysis revealed that decreased miR-206 expression was an independent unfavorable prognostic factor for overall survival. In addition, transfection of miR-206 mimics in HeLa cells was able to reduce cell proliferation, promote cell apoptosis, and inhibit cell invasion and migration.
  
  
  CONCLUSIONS
  miR-206 may act not only as a novel diagnostic and prognostic marker, but also as a potential target for molecular therapy of cervical cancer.
authors.forename: S
authors.initials: S
authors.lastname: Ling
authors.forename: M
authors.initials: M
authors.lastname: Ruiqin
authors.forename: Z
authors.initials: Z
authors.lastname: Guohong
authors.forename: S
authors.initials: S
authors.lastname: Bing
authors.forename: C
authors.initials: C
authors.lastname: Yanshan
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: C546087:MIRN206 microRNA, human; D035683:MicroRNAs