title: Optimization of liquid-state fermentation conditions for the glyphosate degradation enzyme production of strain Aspergillus oryzae by ultraviolet mutagenesis.
abstract: This study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. Spore from Aspergillus oryzae A-F02 was subjected to ultraviolet mutagenesis. Single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. Four mutant strains were obtained and named as FUJX 001, FUJX 002, FUJX 003, and FUJX 004, in which FUJX 001 gave the highest total enzyme activity. Starch concentration at 0.56%, GP concentration at 1,370 mg/l, initial pH at 6.8, and temperature at 30°C were the optimum conditions for the improved glyphosate degradation endoenzyme production of A. oryzae FUJX 001. Under these conditions, the experimental endoenzyme activity was 784.15 U/100 ml fermentation liquor. The result (784.15 U/100 ml fermentation liquor) was approximately 14-fold higher than that of the original strain. The result highlights the potential of glyphosate degradation enzyme to degrade glyphosate.
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Gui-Ming
authors.initials: GM
authors.lastname: Fu
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Ru-Yi
authors.initials: RY
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Kai-Min
authors.initials: KM
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Yuan
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b Sino-German Food Engineering Center , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Cheng-Mei
authors.initials: CM
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|c Food Science College, Nanchang University , Nanchang , China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wan
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005285:Fermentation; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D016296:Mutagenesis / Q000528:radiation effects*; D014466:Ultraviolet Rays; D000097797:Glyphosate
chemical_list: D005998:Glycine; D000097797:Glyphosate
keywords: Aspergillus oryzae; UV mutagenesis; glyphosate degradation endoenzyme; response surface methodology