title: In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP4 protein production.
abstract: OBJECTIVE
  The aim of this study was to determine the antiviral activity of four probiotic metabolites (Lactobacillus and Bifidobacetrium species) against rotavirus in vitro infection monitored by the NSP4 protein production and Ca(2+) release.
  
  
  RESULTS
  The antiviral effect of the metabolites was performed due a comparison between a blocking model and an intracelullar model on MA104 cells, with the response of NSP4 production and Ca(2+) liberation measured by flow cytometry. Significant results were obtained with the metabolites of Lactobacillus casei, and Bifidobacterium adolescentis in the reduction of the protein production (P = 0·04 and P = 0·014) and Ca(2+) liberation (P = 0·094 and P = 0·020) in the intracellular model, which suggests a successful antiviral activity against RV infection.
  
  
  CONCLUSIONS
  This study demonstrates that probiotic metabolites were able to interfere with the final amount of intracellular NSP4 protein and a successful Ca(2+) regulation, which suggests a new approach to the mechanism exerted by probiotics against the rotavirus infection.
  
  
  CONCLUSIONS
  A novel anti-rotaviral effect exerted by probiotic metabolites monitored by the NSP4 protein during the RV in vitro infection and the effect on the Ca(2+) release is reported; suggesting a reduction on the impact of the infection by decreasing the damage of the cells preventing the electrolyte loss.
authors.affiliation: Laboratorio de Virología, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: N N
authors.initials: NN
authors.lastname: Olaya Galán
authors.affiliation: Laboratorio de Virología, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: J C
authors.initials: JC
authors.lastname: Ulloa Rubiano
authors.affiliation: Departamento de Matemáticas, Facultad de Ciencias, Universidad El Bosque, Bogotá, Colombia.
authors.forename: F A
authors.initials: FA
authors.lastname: Velez Reyes
authors.affiliation: Laboratorio de Virología, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: K P
authors.initials: KP
authors.lastname: Fernandez Duarte
authors.affiliation: Laboratorio de Virología, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: S P
authors.initials: SP
authors.lastname: Salas Cárdenas
authors.affiliation: Laboratorio de Virología, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: M F
authors.initials: MF
authors.lastname: Gutierrez Fernandez
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000070005:Bifidobacterium adolescentis / Q000378:metabolism*; D002460:Cell Line; D006023:Glycoproteins / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D008253:Macaca mulatta; D019936:Probiotics / Q000494:pharmacology* / Q000627:therapeutic use; D012401:Rotavirus / Q000187:drug effects* / Q000378:metabolism; D012400:Rotavirus Infections / Q000188:drug therapy / Q000821:virology; D014118:Toxins, Biological / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins
keywords: Bifidobacterium adolescentis; Lactobacillus casei; NSP4 protein; antiviral effect; probiotic metabolites; rotavirus