title: Deciphering the microRNA transcriptome of skeletal muscle during porcine development.
abstract: MicroRNAs (miRNAs) play critical roles in many important biological processes, such as growth and development in mammals. Various studies of porcine muscle development have mainly focused on identifying miRNAs that are important for fetal and adult muscle development; however, little is known about the role of miRNAs in middle-aged muscle development. Here, we present a comprehensive investigation of miRNA transcriptomes across five porcine muscle development stages, including one prenatal and four postnatal stages. We identified 404 known porcine miRNAs, 118 novel miRNAs, and 101 miRNAs that are conserved in other mammals. A set of universally abundant miRNAs was found across the distinct muscle development stages. This set of miRNAs may play important housekeeping roles that are involved in myogenesis. A short time-series expression miner analysis indicated significant variations in miRNA expression across distinct muscle development stages. We also found enhanced differentiation- and morphogenesis-related miRNA levels in the embryonic stage; conversely, apoptosis-related miRNA levels increased relatively later in muscle development. These results provide integral insight into miRNA function throughout pig muscle development stages. Our findings will promote further development of the pig as a model organism for human age-related muscle disease research.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Mai
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Novogene Bioinformatics Institute , Beijing , People's Republic of China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Wenyao
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Qianzi
authors.initials: Q
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Jideng
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: An'an
authors.initials: A
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Novogene Bioinformatics Institute , Beijing , People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Novogene Bioinformatics Institute , Beijing , People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Aquaculture, Southwest University at Rongchang , Chongqing , People's Republic of China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , People's Republic of China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
keywords: Middle-aged; Muscle development; Porcine; Postnatal; Prenatal; miRNA