title: Immunoregulatory activities of polysaccharides from mung bean.
abstract: Ultrasonic treatment was performed on water-extractable polysaccharides from the seed of mung beans. Purified by anion-exchange and gel filtration chromatography, MWP-1' and MWP-2' were obtained. Average molecular weights (Mws) of MWP-1' and MWP-2' were 68.4 kDa, and 52.4 kDa, respectively. Monosaccharides components analysis indicated that MWP-1' was composed of Rha, Ara, Man and Gal in a molar percent of 0.4:2.6:5.3:0.7. MWP-2' was composed of Ara, Man, Gal and Glc in a molar percent of 0.5:1.4:2.1:0.4. In vitro study showed that both polysaccharides samples were able to stimulate the production of secretory molecules (NO, TNF-α and IL-6) of RAW264.7 murine macrophages in a dosage dependent manner. MWP-2' seemed to be the most potent and induced significantly higher the NO production. These findings suggest that the ultrasonic treatment polysaccharides isolated in our study have immune potentiation effects on macrophages.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing 100081, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Animal Science Unit, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing 100081, People's Republic of China. Electronic address: renguixing@caas.cn.
authors.forename: Guixing
authors.initials: G
authors.lastname: Ren
mesh_terms: D000818:Animals; D002460:Cell Line; D007887:Fabaceae; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000069453:Ultrasonic Waves
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D009005:Monosaccharides; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D009569:Nitric Oxide
keywords: Mung bean; Ultrasonic; Water-extractable polysaccharide