title: Evidence for enhanced collagen type III deposition focally in the territorial matrix of osteoarthritic hip articular cartilage.
abstract: To determine if type III collagen is concentrated in the chymotrypsin-extractable collagen pool from osteoarthritic articular cartilage to assess its potential as a biomarker of Osteoarthritis (OA) pathogenic mechanisms.
  
  
  
  Full thickness articular cartilage from grossly normal surfaces was analyzed from femoral heads, obtained at hip replacement surgery, from OA (n = 10) and fracture (n = 10) patients. Collagen, extracted by α-chymotrypsin, was characterized by SDS-PAGE/Western blot analysis, ELISA and immunohistochemistry using monoclonal antibodies specific to collagens types II and III.
  
  
  
  α-Chymotrypsin extracted more collagen from OA than control cartilage. The extractable pool included collagen types II and III from both OA and control hips. Importantly, OA cartilage contained 6-fold more collagen type III than control cartilage, based on ELISA. The estimated total tissue ratio of collagen III/II was in the 1-10% range for individual OA cartilage samples, based on pepsin-solubilized collagen using SDS-PAGE densitometry. Collagen type III N-propeptide trimers were the main molecular fragments seen on Western blot analysis of OA and control extracts. The chymotrypsin-extracted type II collagen gave primarily full-length α1(II) chains and chain fragments of α1(II) on Western blot analysis from both OA and control tissues. Immunohistochemistry showed that type III collagen was more concentrated in the upper half of OA cartilage and in the territorial matrix around individual chondrocytes and chondrocyte clusters.
  
  
  
  The findings confirm that collagen type III deposition occurs in adult articular cartilage but significantly more pronounced in osteoarthritic joints, presenting a potential marker of matrix repair or pathobiology.
authors.affiliation: Lund University, Department of Clinical Sciences Lund, Orthopedics, Lund, Sweden. Electronic address: shahrzad.hosseininia@med.lu.se.
authors.forename: S
authors.initials: S
authors.lastname: Hosseininia
authors.affiliation: Department of Orthopedics & Sports Medicine, University of Washington, Seattle, WA, United States. Electronic address: maweis@uw.edu.
authors.forename: M A
authors.initials: MA
authors.lastname: Weis
authors.affiliation: Department of Orthopedics & Sports Medicine, University of Washington, Seattle, WA, United States. Electronic address: jyotirai@uw.edu.
authors.forename: J
authors.initials: J
authors.lastname: Rai
authors.affiliation: Department of Orthopedics & Sports Medicine, University of Washington, Seattle, WA, United States. Electronic address: lammykim@gmail.com.
authors.forename: L
authors.initials: L
authors.lastname: Kim
authors.affiliation: Department of Orthopedics & Sports Medicine, University of Washington, Seattle, WA, United States. Electronic address: funks@uw.edu.
authors.forename: S
authors.initials: S
authors.lastname: Funk
authors.affiliation: Lund University, Department of Clinical Sciences Lund, Orthopedics, Lund, Sweden. Electronic address: leif.dahlberg@med.lu.se.
authors.forename: L E
authors.initials: LE
authors.lastname: Dahlberg
authors.affiliation: Department of Orthopedics & Sports Medicine, University of Washington, Seattle, WA, United States. Electronic address: deyre@uw.edu.
authors.forename: D R
authors.initials: DR
authors.lastname: Eyre
mesh_terms: D002358:Cartilage, Articular; D019902:Chondrocytes; D002918:Chymotrypsin; D024043:Collagen Type II; D024061:Collagen Type III; D006801:Humans; D010003:Osteoarthritis
chemical_list: D024043:Collagen Type II; D024061:Collagen Type III; D002918:Chymotrypsin; C117608:alpha-chymotrypsin
keywords: Articular cartilage; Collagen type II and type III; Hip osteoarthritis