title: Gastric cardia adenocarcinoma microRNA profiling in Chinese patients.
abstract: Gastric cardia adenocarcinoma (GCA), which occurs at the gastroesophageal boundary, is one of the most malignant types of cancer. Over the past 30 years, the incidence of GCA has increased by approximately sevenfold, which has a more substantial increase than that of many other malignancies. However, as previous studies mainly focus on non-cardia gastric cancer, until now, the mechanisms behind GCA remain largely unknown. MicroRNAs (miRNAs) have been shown to play pivotal roles in carcinogenesis. To gain insight into the molecular mechanisms regulated by miRNAs in GCA development, we investigated miRNA expression profiles using 81 pairs of primary GCAs and corresponding non-tumorigenic tissues. First, 21 pairs of samples were used for microarray analysis, and then another 60 pairs of samples were used for further analysis. Our results showed that 464 miRNAs (237 upregulated, 227 downregulated, false discovery rate FDR <0.05) were differently expressed between GCA and non-tumor tissues. Pearson test and pathway analysis revealed that these dysregulated miRNA correlated coding RNAs may have effects on several cancer-related pathways. Four miRNAs (miR-1244, miR-135b-5p, miR-3196, and miR-628-3p) were found to be associated with GCA differentiation. One miRNA, miR-196a-5p, was found to be associated with age of GCA onset. Further, survival analysis showed that the expression level of miR-135b-5p was associated with GCA survival. Taken together, our study first provided the genome-wide expression profiles of miRNA in GCA and will be good help for further functional studies.
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Shegan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Oncology, Anyang People's Hospital, Anyang, 471500, China.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Fudan-Zhangjiang Center for Clinical Genomics, Zuchongzhi Road 899, Shanghai, 201203, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Ruinuo
authors.initials: R
authors.lastname: Jia
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Liang
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Su
authors.affiliation: Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China.
authors.forename: Jiangman
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China. bpeng@biotecan.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China. samfeng137@hotmail.com.|Henan University of Science and Technology, Jing hua Road 24, Luoyang, 471500, China. samfeng137@hotmail.com.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Feng
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000556:secondary; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Differentiation; Gastric cardia adenocarcinoma; Microarray; miR135b-5p; microRNA