title: Resolution of Site-Specific Conformational Heterogeneity in Proline-Rich Molecular Recognition by Src Homology 3 Domains.
abstract: Conformational heterogeneity and dynamics are increasingly evoked in models of protein molecular recognition but are challenging to experimentally characterize. Here we combine the inherent temporal resolution of infrared (IR) spectroscopy with the spatial resolution afforded by selective incorporation of carbon-deuterium (C-D) bonds, which provide frequency-resolved absorptions within a protein IR spectrum, to characterize the molecular recognition of the Src homology 3 (SH3) domain of the yeast protein Sho1 with its cognate proline-rich (PR) sequence of Pbs2. The IR absorptions of C-D bonds introduced at residues along a peptide of the Pbs2 PR sequence report on the changes in the local environments upon binding to the SH3 domain. Interestingly, upon forming the complex the IR spectra of the peptides labeled with C-D bonds at either of the two conserved prolines of the PXXP consensus recognition sequence show more absorptions than there are C-D bonds, providing evidence for the population of multiple states. In contrast, the NMR spectra of the peptides labeled with (13)C at the same residues show only single resonances, indicating rapid interconversion on the NMR time scale. Thus, the data suggest that the SH3 domain recognizes its cognate peptide with a component of induced fit molecular recognition involving the adoption of multiples states, which have previously gone undetected due to interconversion between the populated states that is too fast to resolve using conventional methods.
authors.affiliation: Department of Chemistry, Indiana University , 800 East Kirkwood, Bloomington, Indiana 47405, United States.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Horness
authors.affiliation: Department of Chemistry, Indiana University , 800 East Kirkwood, Bloomington, Indiana 47405, United States.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Basom
authors.affiliation: Department of Chemistry, Indiana University , 800 East Kirkwood, Bloomington, Indiana 47405, United States.
authors.forename: John P
authors.initials: JP
authors.lastname: Mayer
authors.affiliation: Department of Chemistry, Indiana University , 800 East Kirkwood, Bloomington, Indiana 47405, United States.
authors.forename: Megan C
authors.initials: MC
authors.lastname: Thielges
mesh_terms: D001665:Binding Sites; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D011392:Proline / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D018909:src Homology Domains
chemical_list: D011506:Proteins; D011392:Proline