title: Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis.
abstract: Bark beetles (Coleoptera: Scolytinae) are pests of many forests around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant pest of western North American pine forests. The MPB is able to overcome the defences of pine trees through pheromone-assisted aggregation that results in a mass attack of host trees. These pheromones, both male and female produced, are believed to be biosynthesized in the midgut and/or fat bodies of these insects. We used metabolite analysis, quantitative proteomics (iTRAQ) and transcriptomics (RNA-seq) to identify proteins and transcripts differentially expressed between sexes and between tissues when treated with juvenile hormone III. Juvenile hormone III induced frontalin biosynthesis in males and trans-verbenol biosynthesis in females, as well as affected the expression of many proteins and transcripts in sex- and tissue-specific ways. Based on these analyses, we identified candidate genes involved in the biosynthesis of frontalin, exo-brevicomin, and trans-verbenol pheromones.
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4. Electronic address: ckeeling@alumni.sfu.ca.
authors.forename: Christopher I
authors.initials: CI
authors.lastname: Keeling
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4.
authors.forename: Maria
authors.initials: M
authors.lastname: Li
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4.
authors.forename: Harpreet K
authors.initials: HK
authors.lastname: Sandhu
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4.
authors.forename: Hannah
authors.initials: H
authors.lastname: Henderson
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4.
authors.forename: Macaire Man Saint
authors.initials: MM
authors.lastname: Yuen
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4.
authors.forename: Jörg
authors.initials: J
authors.lastname: Bohlmann
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism*; D008297:Male; D055442:Metabolome; D010675:Pheromones / Q000096:biosynthesis*; D020543:Proteome; D059467:Transcriptome
chemical_list: D010675:Pheromones; D020543:Proteome
keywords: Cytochrome P450; Fat bodies; Mevalonate pathway; Midgut