title: Quantitative Profiling of Combinational K27/K36 Modifications on Histone H3 Variants in Mouse Organs.
abstract: The coexisting post-translational modifications (PTMs) on histone H3 N-terminal tails were known to crosstalk between each other, indicating their interdependency in the epigenetic regulation pathways. H3K36 methylation, an important activating mark, was recently reported to antagonize with PRC2-mediated H3K27 methylation with possible crosstalk mechanism during transcription regulation process. On the basis of our previous studies, we further integrated RP/HILIC liquid chromatography with MRM mass spectrometry to quantify histone PTMs from various mouse organs, especially the combinatorial K27/K36 marks for all three major histone H3 variants. Despite their subtle difference in physicochemical properties, we successfully obtained decent separation and high detection sensitivity for both histone H3.3 specific peptides and histone H3.1/3.2 specific peptides. In addition, the overall abundance of H3.3 can be quantified simultaneously. We applied this method to investigate the pattern of the combinatorial K27/K36 marks for all three major histone H3 variants across five mouse organs. Intriguing distribution differences were observed not only between different H3 variants but also between different organs. Our data shed the new insights into histone codes functions in epigenetic regulation during cell differentiation and developmental process.
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Chen
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Rijing
authors.initials: R
authors.lastname: Liao
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Counde
authors.initials: C
authors.lastname: Oyang
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: En
authors.initials: E
authors.lastname: Li
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Zeng
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Shaolian
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.affiliation: China Novartis Institutes for BioMedical Research Co. Ltd. , Building 8, Lane 898 Halei Road, Shanghai 201203, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D006657:Histones; D008239:Lysine
keywords: histone H3 variants; mouse organs; post-translational modifications; quantification