title: [Correlation of gene expression related to amount of ginseng saponin in 15 tissues and 6 kinds of ginseng saponin biosynthesis].
abstract: Fifteen tissues of 4-year-old fruit repining stage Jilin ginseng were chosen as materials, six kinds of monomer saponins (ginsenosides Rg1, Re, Rb1, Rc, Rb2 and Rd) content in 15 tissues was measured by HPLC and vanillin-sulfuric acid method. The relative expression of FPS, SQS, SQE, OSC, β-AS and P450 genes in 15 tissues was analyzed by real-time PCR. The correlations between ginseng saponin content in 15 tissues of Jilin ginseng and biosynthetic pathway -related genes were obtained. The results showed that was a synergistic increase and decrease trend of positive linear correlation among six kinds of monomer saponin content, and there was a significantly (P < 0.01) positive correlation between monomer saponin content and total saponins content. Monomer saponin content and 6 kinds of enzyme gene correlation were different. Biosynthesis of ginseng total saponins and monomer saponin were regulated by six kinds of participation ginsenoside biosynthesis enzyme genes, the expression of these six kinds of genes in different tissues of ginseng showed collaborative increase and decrease trend, and regulated biosynthesis of ginseng ginsenoside by group coordinative manner.
authors.forename: Kang-yu
authors.initials: KY
authors.lastname: Wang
authors.forename: Mei-ping
authors.initials: MP
authors.lastname: Zhang
authors.forename: Chuang
authors.initials: C
authors.lastname: Li
authors.forename: Shi-cui
authors.initials: SC
authors.lastname: Jiang
authors.forename: Rui
authors.initials: R
authors.lastname: Yin
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis*; D020869:Gene Expression Profiling; D005894:Panax / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018514:Plant Structures / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000032:analysis* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D012503:Saponins