title: Tenggerimyces flavussp. nov., isolated from soil in a karst cave, and emended description of the genusTenggerimyces.
abstract: A novel actinomycete, designated strain S6R2A4-9T, was isolated from a soil sample collected from a karst cave in Henan Province, China, and subjected to a polyphasic taxonomic study. This isolate grew optimally at 25-28 °C, pH 6.5-8.0 and in the absence of NaCl. The substrate mycelium of the isolate was well developed with irregular branches. Aerial mycelium fragmented into long, rod-shaped elements. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain S6R2A4-9Tresided in the cluster of the genusTenggerimyceswithin the familyNocardioidaceaeand shared the highest 16S rRNA gene sequence similarity (98.98 %) withTenggerimyces mesophilusI12A-02601T. The G+C content of the genomic DNA was 67.0 mol%. The strain contained glucose, ribose and xylose in its whole-cell hydrolysates. Strain S6R2A4-9Tpossessed a novel variation of peptidoglycan derived from the type A1γmeso-Dpm-direct. The polar lipids consisted of diphosphatidylglycerol,N-acetylglucosamine-containing phospholipid, phosphatidylinositol mannoside, phosphatidylglycerol, phosphoglycolipids and glycolipids. The predominant menaquinones were MK-10(H6) and MK-10(H8). The major fatty acids were C16 : 0, iso-C16 : 0and 10-methyl C17 : 0. The level of DNA-DNA relatedness between strain S6R2A4-9TandT. mesophilusI12A-02601Twas 27.6 ± 3.0 %, which was low enough to indicate that the strain represents a distinct species of the genusTenggerimyces. On the basis of the polyphasic taxonomic evidence, a novel species,Tenggerimyces flavussp. nov., is proposed. The type strain of the novel species is S6R2A4-9T( = DSM 28944T= CGMCC 4.7241T).
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.|College of laboratory Medical Science, Hebei North University, Zhangjiakou 075000, PR China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Dai
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Jia-Meng
authors.initials: JM
authors.lastname: Liu
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Cheng-Hang
authors.initials: CH
authors.lastname: Sun