title: Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.
abstract: Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Simerpreet
authors.initials: S
authors.lastname: Kaur
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Vibhuti
authors.initials: V
authors.lastname: Rana
authors.affiliation: Department of Microbiology, King George Medical University, Lucknow 226003, Uttar Pradesh, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Singh
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Garima
authors.initials: G
authors.lastname: Trivedi
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Anand
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Amanpreet
authors.initials: A
authors.lastname: Kaur
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors.forename: Pawan
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Department of Microbiology, King George Medical University, Lucknow 226003, Uttar Pradesh, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Jain
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India. Electronic address: charu@imtech.res.in.
authors.forename: Charu
authors.initials: C
authors.lastname: Sharma
mesh_terms: D020121:5' Untranslated Regions; D000123:Acetyltransferases; D000942:Antigens, Bacterial / Q000235:genetics; D000995:Antitubercular Agents / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007194:India; D007612:Kanamycin / Q000627:therapeutic use*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011401:Promoter Regions, Genetic; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D020121:5' Untranslated Regions; D000942:Antigens, Bacterial; D000995:Antitubercular Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007612:Kanamycin; D000123:Acetyltransferases; C430563:Eis protein, Mycobacterium tuberculosis
keywords: Aminoglycosides; MDR-TB; Molecular characterization; XDR-TB