title: Intraoperative subserosal approach to label sentinel nodes in intermediate and high-risk endometrial cancer.
abstract: METHODS
  prospective experimental study.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate feasibility and reliabil- ity of in vivo sentinel lymph node (SLN) mapping in patients with endometrial cancer and to verify a modified method of application of subserosal blue dye. Detection substance was applied subserosally in the uterine edges vicinity the round ligament of uterus and uterine vessels in the isthmic portion of the uterus.
  
  
  METHODS
  Eighteen patients with intermediate and high-risk endome- trial cancer Stages I-II were subjected to staging laparotomy with intraoperative detection of SLNs and subsequent completion of the pelvic and para-aortic lymphadenectomies. Harvested SLN was routinely examined by classical haematoxylin eosin staining and in case of negativity, immunohistochemistry with anti-keratin antibodies AEl/AE3 was applied.
  
  
  RESULTS
  Total of 773 lymph nodes were removed in 18 patients: pelvic 420 (54%) and para-aortic 353 (46%). SLNs were detected in 16 of 18 patients totalling 59 nodes (7.6% of all nodes). Forty-eight were identified in the pelvic area (81%) and 11 nodes (19%) in the para-aortic area. Three metastatic SLNs were found in two patients (11%). No false negative nodes were demonstrated.
  
  
  CONCLUSIONS
  Experimental study results indicate that the proposed modified approach to label SLNs is applicable. The presented modified approach brings the highest added value namely in women with a myomatous uterus and scars from previous surgical procedures on the uterus.
authors.forename: P
authors.initials: P
authors.lastname: Valha
authors.forename: E
authors.initials: E
authors.lastname: Kucera
authors.forename: P
authors.initials: P
authors.lastname: Sak
authors.forename: O
authors.initials: O
authors.lastname: Stepanek
authors.forename: M
authors.initials: M
authors.lastname: Michal
mesh_terms: D000368:Aged; D016889:Endometrial Neoplasms / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D008875:Middle Aged; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*