title: An aqueous extract from toad skin prevents gelatinase activities derived from fetal serum albumin and serum-free culture medium of human breast carcinoma MDA-MB-231 cells.
abstract: An aqueous extract from toad skin, cinobufacini, has been known to possess anticancer ability. The present study examined effect of toad skin extract on activity of gelatinases including matrix metalloproteinases-2 and -9 which play an important role in invasion of carcinoma cells. Gelatinase activities derived from fetal serum albumin and culture medium of human breast carcinoma cell line MDA-MB-231 were significantly prevented in the presence of toad skin extract. The inhibitory activity was found in water-soluble fraction of the extract prepared by the Bligh & Dyer method but not in CHCl(3)-soluble lipid fraction. These results suggest that an aqueous extract from toad skin contains a water-soluble substance possessing a potent ability to prevent gelatinase activity. In conclusion, the water-soluble substance in toad skin extract cinobufacini may be able to regulate cancer cell migration accelerated by matrix metalloproteinases.
authors.affiliation: Department of Applied Biochemistry, Tokai University.
authors.forename: Munehiro
authors.initials: M
authors.lastname: Nakata
authors.forename: Shota
authors.initials: S
authors.lastname: Kawaguchi
authors.forename: Ayami
authors.initials: A
authors.lastname: Oikawa
authors.forename: Akito
authors.initials: A
authors.lastname: Inamura
authors.forename: Shunki
authors.initials: S
authors.lastname: Nomoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Miyai
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nonaka
authors.forename: Saeko
authors.initials: S
authors.lastname: Ichimi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujita-Yamaguchi
authors.forename: Chuan
authors.initials: C
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
mesh_terms: D000664:Amphibian Venoms / Q000494:pharmacology*; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D016895:Culture Media, Serum-Free; D005260:Female; D018093:Gelatinases / Q000037:antagonists & inhibitors* / Q000502:physiology; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D000664:Amphibian Venoms; D016895:Culture Media, Serum-Free; D012709:Serum Albumin; C545813:huachansu; D018093:Gelatinases