title: The MVA pathway genes expressions and accumulation of celastrol in Tripterygium wilfordii suspension cells in response to methyl jasmonate treatment.
abstract: Celastrol is an important bioactive triterpenoid in traditional Chinese medicinal plant, Tripterygium wilfordii. Methyl Jasmonate (MJ) is a common plant hormone which can regulate the secondary metabolism in higher plants. In this study, the mevalonate (MVA) pathway genes in T. wilfordii were firstly cloned. The suspension cells of T. wilfordii were elicited by MJ, and the expressions of MVA pathway genes were all enhanced in different levels ranging from 2.13 to 22.33 times of that at 0 h. The expressions were also enhanced compared with the CK group separately. The accumulation of celastrol in the suspension cells after the treatment was quantified and co-analyzed with the genes expression levels. The production of celastrol was significantly increased to 0.742 mg g(-1) after MJ treatment in 288 h which is consistent with the genes expressions. The results provide plenty of gene information for the biosynthesis of terpenoids in T. wilfordii and a viable way to improve the accumulation of celastrol in T. wilfordii suspension cells.
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Zhao
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.|b National Resource Center for Chinese Materia Medica , China Academy of Chinese Medical Sciences , Beijing 100700 , China.
authors.forename: Ping
authors.initials: P
authors.lastname: Su
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.|b National Resource Center for Chinese Materia Medica , China Academy of Chinese Medical Sciences , Beijing 100700 , China.
authors.forename: Yu-Ru
authors.initials: YR
authors.lastname: Tong
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Hu
authors.affiliation: b National Resource Center for Chinese Materia Medica , China Academy of Chinese Medical Sciences , Beijing 100700 , China.
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.affiliation: a School of Traditional Chinese Medicine , Capital Medical University , Beijing 100069 , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D008798:Mevalonic Acid / Q000378:metabolism; D015394:Molecular Structure; D054883:Oxylipins / Q000494:pharmacology*; D053978:Pentacyclic Triterpenes; D013729:Terpenes / Q000378:metabolism; D029757:Tripterygium / Q000737:chemistry* / Q000235:genetics*; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D053978:Pentacyclic Triterpenes; D013729:Terpenes; D014315:Triterpenes; C072239:methyl jasmonate; C050414:celastrol; D008798:Mevalonic Acid
keywords: Celastrol; Tripterygium wilfordii; methyl jasmonate; mevalonate pathway; triterpenoids