title: Growth and consumption of L-malic acid in wine-like medium by acclimated and non-acclimated cultures of Patagonian Oenococcus oeni strains.
abstract: Five Oenococcus oeni strains, selected from spontaneous malolactic fermentation (MLF) of Patagonic Pinot noir wine, were assessed for their use as MLF starter cultures. After the individual evaluation of tolerance to some stress conditions, usually found in wine (pH, ethanol, SO2, and lysozyme), the behavior of the strains was analyzed in MLO broth with 14 % ethanol and pH 3.5 in order to test for the synergistic effect of high ethanol level and low pH and, finally, in a wine-like medium. Although the five strains were able to grow in MLO broth under low pH and/or high ethanol, they must be acclimated to grow in a wine-like medium. Additionally, glycosidase and tannase activities were evaluated, showing differences among the strains. The potential of the strains to ferment citrate was tested and two of the five strains showed the ability to metabolize this substrate. We did not detect the presence of genes encoding histidine, tyrosine descarboxylase, and putrescine carbamoyltransferase. All the strains tested exhibited good growth capacity and ability to consume L-malic acid in a wine-like medium after cell acclimation, and each of them showed a particular enzyme profile, which might confer different organoleptic properties to the wine.
authors.affiliation: Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina. bbferrada@unq.edu.ar.
authors.forename: Bárbara Mercedes
authors.initials: BM
authors.lastname: Bravo-Ferrada
authors.affiliation: Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina.|Laboratorio de Biointerfaces y Sistemas Biomiméticos - Universidad Nacional de Santiago del Estero (UNSE) y Centro de Investigaciones y Transferencia de Santiago del Estero (CITSE) - CONICET y UNSE, Santiago del Estero, Argentina.
authors.forename: Axel
authors.initials: A
authors.lastname: Hollmann
authors.affiliation: Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina.
authors.forename: Natalia
authors.initials: N
authors.lastname: Brizuela
authors.affiliation: Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina.
authors.forename: Danay Valdés
authors.initials: DV
authors.lastname: La Hens
authors.affiliation: Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Tymczyszyn
authors.affiliation: Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina.
authors.forename: Liliana
authors.initials: L
authors.lastname: Semorile
mesh_terms: D000222:Adaptation, Physiological; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008293:Malates / Q000378:metabolism*; D008962:Models, Theoretical; D056585:Oenococcus / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D014920:Wine / Q000382:microbiology*
chemical_list: D003470:Culture Media; D008293:Malates; D000431:Ethanol; C030298:malic acid