title: Determination of phospholipids in milk using a new phosphodiester stationary phase by liquid chromatography-matrix assisted desorption ionization mass spectrometry.
abstract: A methodology employing high performance liquid chromatography coupled with matrix-assisted laser desorption and ionization time-of-flight mass spectrometry has been utilized to determine the quality of phospholipid classes. Home-made phosphoester chemically bonded stationary phase containing diol, phosphate and octadecyl groups (Diol-P-C18) has been employed in the separation of polar lipids from milk. Each phospholipid fraction was collected manually and identified by MALDI-TOF MS.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Street, 87-100 Torun, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Walczak
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Street, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Wileńska 4, 87-100 Torun, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Pomastowski
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Street, 87-100 Torun, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Bocian
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Street, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Wileńska 4, 87-100 Torun, Poland. Electronic address: bbusz@chem.umk.pl.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008892:Milk / Q000737:chemistry*; D010755:Organophosphates; D010743:Phospholipids / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D010755:Organophosphates; D010743:Phospholipids
keywords: Fatty acids; HPLC; MALDI-TOF MS; Phosphodiester stationary phase; Phospholipids