title: Dynamics of Bacterial Community Composition in the Malaria Mosquito's Epithelia.
abstract: The Anopheles midgut hosts diverse bacterial communities and represents a complex ecosystem. Several evidences indicate that mosquito midgut microbiota interferes with malaria parasite transmission. However, the bacterial composition of salivary glands and ovaries, two other biologically important tissues, has not been described so far. In this study, we investigated the dynamics of the bacterial communities in the mosquito tissues from emerging mosquitoes until 8 days after a blood meal containing Plasmodium falciparum gametocytes and described the temporal colonization of the mosquito epithelia. Bacterial communities were identified in the midgut, ovaries, and salivary glands of individual mosquitoes using pyrosequencing of the 16S rRNA gene. We found that the mosquito epithelia share a core microbiota, but some bacteria taxa were more associated with one or another tissue at a particular time point. The bacterial composition in the tissues of emerging mosquitoes varied according to the breeding site, indicating that some bacteria are acquired from the environment. Our results revealed temporal variations in the bacterial community structure, possibly as a result of the mosquito physiological changes. The abundance of Serratia significantly correlated with P. falciparum infection both in the midgut and salivary glands of malaria challenged mosquitoes, which suggests that interactions occur between microbes and parasites. These bacteria may represent promising targets for vector control strategies. Overall, this study points out the importance of characterizing bacterial communities in malaria mosquito vectors.
authors.affiliation: UMR Maladies Infectieuses Et Vecteurs Écologie, Génétique, Évolution Et Contrôle, IRD 224- Centre National de la Recherche Scientifique 5290- UM1- UM2Montpellier, France; Laboratoire d'entomologie médicale, OCEAC-IRDYaoundé, Cameroon.
authors.forename: Majoline T
authors.initials: MT
authors.lastname: Tchioffo
authors.affiliation: UMR Maladies Infectieuses Et Vecteurs Écologie, Génétique, Évolution Et Contrôle, IRD 224- Centre National de la Recherche Scientifique 5290- UM1- UM2 Montpellier, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Boissière
authors.affiliation: UMR Maladies Infectieuses Et Vecteurs Écologie, Génétique, Évolution Et Contrôle, IRD 224- Centre National de la Recherche Scientifique 5290- UM1- UM2 Montpellier, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Abate
authors.affiliation: Laboratoire d'entomologie médicale, OCEAC-IRDYaoundé, Cameroon; Faculté de Médecine et des Sciences Pharmaceutiques, Université de DoualaDouala, Cameroon.
authors.forename: Sandrine E
authors.initials: SE
authors.lastname: Nsango
authors.affiliation: Laboratoire d'entomologie médicale, OCEAC-IRD Yaoundé, Cameroon.
authors.forename: Albert N
authors.initials: AN
authors.lastname: Bayibéki
authors.affiliation: Laboratoire d'entomologie médicale, OCEAC-IRD Yaoundé, Cameroon.
authors.forename: Parfait H
authors.initials: PH
authors.lastname: Awono-Ambéné
authors.affiliation: Faculté des Sciences, Centre National de la Recherche Scientifique UMR 7138Nice, France; Laboratoire de Biologie Virtuelle, Faculté des Sciences, UMR 713, Université de NiceNice, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Christen
authors.affiliation: UMR Maladies Infectieuses Et Vecteurs Écologie, Génétique, Évolution Et Contrôle, IRD 224- Centre National de la Recherche Scientifique 5290- UM1- UM2Montpellier, France; Laboratoire d'entomologie médicale, OCEAC-IRDYaoundé, Cameroon.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Gimonneau
authors.affiliation: UMR Maladies Infectieuses Et Vecteurs Écologie, Génétique, Évolution Et Contrôle, IRD 224- Centre National de la Recherche Scientifique 5290- UM1- UM2Montpellier, France; Laboratoire d'entomologie médicale, OCEAC-IRDYaoundé, Cameroon.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Morlais
keywords: bacterial communities; dynamics; malaria vector; mosquito tissues; pyrosequencing