title: Phytotoxicity of chiral herbicide bromacil: Enantioselectivity of photosynthesis in Arabidopsis thaliana.
abstract: With the wide application of chiral herbicides and the frequent detection of photosystem II (PSII) herbicides, it is of great importance to assess the direct effects of PSII herbicides on photosynthesis in an enantiomeric level. In the present study, the enantioselective phytotoxicity of bromacil (BRO), typical photosynthesis inhibition herbicide, on Arabidopsis thaliana was investigated. The results showed that S-BRO exhibited a greater inhibition of electron transmission in photosystem I (PSI) of A. thaliana than R-BRO by inhibiting the transcription of fnr 1. S-BRO also changed the chlorophyll fluorescence parameters Y (II), Y (NO), and Y (NPQ) to a greater extent than R-Bro. Transcription of genes psbO2, Lhcb3 and Lhcb6 was down-regulated in an enantioselective rhythm and S-BRO caused more serious influence, indicating that S-BRO did worse damage to the photosystem II (PSII) of A. thaliana than R-BRO. This study suggested that S-BRO disturbed the photosynthesis of plants to a larger extent than R-BRO and provided a new sight to evaluate the phytotoxicity of chiral herbicides.
authors.affiliation: MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zunwei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zou
authors.affiliation: MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Research Center of Analysis and Measurement, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Meichao
authors.initials: M
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: wenyuezhong@zju.edu.cn.
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Wen
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000502:physiology; D001976:Bromouracil / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects*
chemical_list: D006540:Herbicides; D001976:Bromouracil; C004590:bromacil
keywords: Chiral; Enantioselectivity; Herbicide; Photosynthesis; Phytotoxicity