title: Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice.
abstract: The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibodies were detected by ELISA and an agglutination test. After the second immunization, the antibody level increased and the rate of immune protection against the Ee strain was 70 and 91.7% in the subunit bacterin and bacterin groups, respectively. Therefore, the mixed subunit bacterin provided good protection against the homologous Ee strain, which provides a basis for further research, into high-efficacy vaccines against porcine edema disease.
authors.affiliation: College of Animal Science, Yangtze University, JingZhou, HuBei, China.|Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Center for Animal Disease Control and Prevention of China, Beijing, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: College of Animal Science, Yangtze University, JingZhou, HuBei, China.|Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Hu
authors.affiliation: College of Animal Science, Yangtze University, JingZhou, HuBei, China.|Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
authors.forename: L W
authors.initials: LW
authors.lastname: Guo
authors.affiliation: College of Animal Science, Yangtze University, JingZhou, HuBei, China.|Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.|State Key Laboratory of Microbiology, Huazhong Agricultural University, WuHan, HuBei, China.
authors.forename: G P
authors.initials: GP
authors.lastname: Liu
authors.affiliation: Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.|State Key Laboratory of Microbiology, Huazhong Agricultural University, WuHan, HuBei, China.
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.affiliation: Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.|State Key Laboratory of Microbiology, Huazhong Agricultural University, WuHan, HuBei, China.
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics; D004488:Edema Disease of Swine / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D007114:Immunization; D051379:Mice; D021122:Protein Subunits / Q000235:genetics; D022641:Shiga Toxin 2 / Q000008:administration & dosage / Q000235:genetics*; D013552:Swine / Q000382:microbiology
chemical_list: D001428:Bacterial Vaccines; D021122:Protein Subunits; D022641:Shiga Toxin 2