title: Glycogen content in hepatocytes is related with their size in normal rat liver but not in cirrhotic one.
abstract: Hepatocytes differ from one another by the degree of the ploidy, size, position in the liver lobule, and level of the DNA-synthetic processes. It is believed, that the cell size exerts substantial influence on the metabolism of the hepatocytes and the glycogen content in them. The aim of the present study was to test this hypothesis.
  
  
  
  Dry weight of hepatocytes, their ploidy and glycogen content were determined in the normal and the cirrhotic rat liver. Liver cirrhosis in rats was produced by chronic inhalation of CCl4 vapours in the course of 6 months. A combined cytophotometric method was used. Dry weight of the cell, its glycogen and DNA content were successively measured on a mapped preparation.
  
  
  
  Hepatocytes of each ploidy class in the normal and the cirrhotic rat liver accumulated glycogen at the same rate. In the normal liver, there was a distinct correlation between the size of hepatocytes and glycogen content in them. This correlation was observed in each ploidy class, and was especially pronounced in the class of mononucleate tetraploid hepatocytes. In the cirrhotic liver, there was no correlation between the size of the cells and their glycogen content.
  
  
  
  The impairment of liver lobular structure probably explains the observed lack of correlation between hepatocyte size and their glycogen content in the cirrhotic liver. © 2016 International Society for Advancement of Cytometry.
authors.affiliation: Laboratory of Cellular Pathology, Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Bezborodkina
authors.affiliation: Laboratory of Cellular Pathology, Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.
authors.forename: Anna Yu
authors.initials: AY
authors.lastname: Chestnova
authors.affiliation: Laboratory of Cellular Pathology, Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.
authors.forename: Mikhail L
authors.initials: ML
authors.lastname: Vorobev
authors.affiliation: Laboratory of Cellular Pathology, Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.
authors.forename: Boris N
authors.initials: BN
authors.lastname: Kudryavtsev
mesh_terms: D000818:Animals; D048429:Cell Size; D006003:Glycogen / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011003:Ploidies; D051381:Rats
chemical_list: D006003:Glycogen
keywords: dry weight of hepatocytes; glycogen; hepatocytes; liver cirrhosis; ploidy