title: Mitochondrial FOXO3a is involved in amyloid β peptide-induced mitochondrial dysfunction.
abstract: Mitochondrial dysfunction is a hallmark of amyloid β peptide (Aβ)-induced neuronal toxicity in Alzheimer's disease (AD). However, the precise mechanism(s) of Aβ-induced mitochondrial dysfunction has not been fully understood. There is evidence that Forkhead box O3a (FOXO3a) is normally present in neuronal mitochondria. Using HT22 murine hippocampal neuronal cells and primary hippocampal neurons, the present study investigated whether mitochondrial FOXO3a was involved in mitochondrial dysfunction induced by Aβ. It was found that Aβ induced dephosphorylation and mitochondrial translocation of FOXO3a. In addition, Aβ enhanced association of FOXO3a with mitochondrial DNA (mtDNA), causing a decrease in the expression of cytochrome c oxidase subunit 1 (COX1) and the activity of COX. In addition, Aβ-induced mitochondrial dysfunction, indicated by the decrease in 3- (4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion, mitochondrial adenosine triphosphate (ATP) production and COX activity, could be suppressed by knockdown of FOXO3a (FOXO3a-KD). These results provide new insights into the mechanism underlying Aβ-induced neurotoxicity and open up new therapeutic perspectives for AD.
authors.affiliation: Department of Anatomy, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Human Anatomy, Dali University, Dali, Yunnan, 671003, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Anatomy, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China.
authors.forename: Shuilong
authors.initials: S
authors.lastname: Leng
authors.affiliation: Department of Anatomy, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China.
authors.forename: Dahong
authors.initials: D
authors.lastname: Long
authors.affiliation: Department of Anatomy, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China. sallyshi431@126.com.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Luo
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000544:Alzheimer Disease; D016229:Amyloid beta-Peptides / Q000494:pharmacology / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D000071316:Forkhead Box Protein O3 / Q000378:metabolism / Q000502:physiology*; D006624:Hippocampus / Q000473:pathology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D004272:DNA, Mitochondrial; D000071316:Forkhead Box Protein O3; C474033:FoxO3 protein, mouse; D024101:Mitochondrial Proteins; D000255:Adenosine Triphosphate; D011451:Prostaglandin-Endoperoxide Synthases
keywords: Aβ; COX1; FOXO3a; Mitochondria