title: Effects of Low-Protein Diets Supplemented with Ketoacid on Expression of TGF-β and Its Receptors in Diabetic Rats.
abstract: TGF-β 1 has been recognized as a key mediator in DN. This study aimed to observe the effects of low-protein diets supplemented with ketoacid on mRNA and protein expression of TGF-β and TβRI and t TβRII receptors in the renal tissue of diabetic rats. A diabetes model was established in 72 male SD rats. They were then equally randomized to three groups: NPD group, LPD group, and LPD + KA group. Additional 24 male SD rats receiving normal protein diets were used as the control. Eight rats from each group were sacrificed at weeks 4, 8, and 12 after treatment, from which SCr, BUN, serum albumin, and 24 h urinary protein excretion were collected. The expressions of TGF-β 1, TβRI, and TβRII in LPD and LPD + KA groups were significantly lower than those in NPD group and lower in LPD + KA group than those in LPD group. Low-protein diets supplemented with ketoacid have been demonstrated to provide a protective effect on the renal function as represented by reduced SCr, BUN, and urinary protein excretion, probably through downregulating the gene expression of TGF-β 1 and its receptors in LPD + KA group.
authors.affiliation: Department of Nephrology, Hangzhou First People's Hospital, Zhejiang 310006, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Nephrology, Changzheng Hospital, Second Military Medical University and the Kidney Institute of CPLA, Shanghai 200003, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Nephrology, Changzheng Hospital, Second Military Medical University and the Kidney Institute of CPLA, Shanghai 200003, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cheng
authors.affiliation: Department of Nephrology, Hangzhou First People's Hospital, Zhejiang 310006, China.
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Ma
authors.affiliation: Department of Nephrology, Hangzhou First People's Hospital, Zhejiang 310006, China.
authors.forename: Xiang-Cheng
authors.initials: XC
authors.lastname: Xie
authors.affiliation: Department of Osteology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Nephrology, Changzheng Hospital, Second Military Medical University and the Kidney Institute of CPLA, Shanghai 200003, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Hangzhou First People's Hospital, Zhejiang 310006, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Fei
authors.affiliation: Department of Nephrology, Hangzhou First People's Hospital, Zhejiang 310006, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Changzheng Hospital, Second Military Medical University and the Kidney Institute of CPLA, Shanghai 200003, China.
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Mei
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000178:diet therapy* / Q000235:genetics / Q000473:pathology; D003928:Diabetic Nephropathies / Q000178:diet therapy* / Q000235:genetics / Q000473:pathology; D018753:Diet, Protein-Restricted; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D051381:Rats; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta