title: Determination of the minimum fully protective dose of adenovirus-based DIVA vaccine against peste des petits ruminants virus challenge in East African goats.
abstract: Peste des petits ruminants virus (PPRV) causes an economically important disease of sheep and goats, primarily in developing countries. It is becoming the object of intensive international control efforts. Current vaccines do not allow vaccinated and infected animals to be distinguished (no DIVA capability). We have previously shown that recombinant, replication-defective, adenovirus expressing the PPRV H glycoprotein (AdH) gives full protection against wild type PPRV challenge. We have now tested lower doses of the vaccine, as well as AdH in combination with a similar construct expressing the PPRV F glycoprotein (AdF). We show here that, in a local breed of goat in a country where PPR disease is common (Kenya), as little as 10(7) pfu of AdH gives significant protection against PPRV challenge, while a vaccine consisting of 10(8) pfu of each of AdH and AdF gives apparently sterile protection. These findings underline the utility of these constructs as DIVA vaccines for use in PPR control.
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK. barbara.holzer@pirbright.ac.uk.
authors.forename: Barbara
authors.initials: B
authors.lastname: Holzer
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK. geraldine.taylor@pirbright.ac.uk.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Taylor
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK. paulina.rajkonenow@pirbright.ac.uk.
authors.forename: Paulina
authors.initials: P
authors.lastname: Rajko-Nenow
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK. sophia.hodgson@pirbright.ac.uk.
authors.forename: Sophia
authors.initials: S
authors.lastname: Hodgson
authors.affiliation: International Livestock Research Institute, Nairobi, Kenya. e.okoth@cgiar.org.
authors.forename: Edward
authors.initials: E
authors.lastname: Okoth
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK. Rebecca.Herbert@quintiles.com.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Herbert
authors.affiliation: International Livestock Research Institute, Nairobi, Kenya. p.toye@cgiar.org.
authors.forename: Philip
authors.initials: P
authors.lastname: Toye
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK. michael.baron@pirbright.ac.uk.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Baron
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D002522:Chlorocebus aethiops; D006023:Glycoproteins / Q000276:immunology; D015511:Goat Diseases / Q000517:prevention & control* / Q000821:virology; D006041:Goats; D019590:Nucleocapsid Proteins / Q000276:immunology; D029021:Peste-des-Petits-Ruminants / Q000517:prevention & control*; D018107:Peste-des-petits-ruminants virus; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D019590:Nucleocapsid Proteins; D014765:Viral Vaccines