title: Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette.
abstract: We demonstrate a standalone microfluidic chip that allows us to carry out commonly executed antibiotic susceptibility assays in an array of nanoliter droplets. We eliminated the need for automation in performing an exemplary complicated liquid handling assay on a chip. Operations on droplets are hard-wired into the microfluidic chip. The liquid handling protocol can be executed with a simple and commonly available source of flow such as an automatic manual pipette. The system passively prepares a series of dilutions of a chemical compound and mixes them with portions of the sample. The precision of metering, merging, mixing, and splitting of discrete portions of liquid samples is rooted in the passive capillary action in microfluidic traps and not in the precision of dosing with a pipette. We show an exemplary use of the device in the determination of the minimum inhibitory concentration (MIC) of ampicillin against E. coli ATCC 25922.
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. garst@ichf.edu.pl and University of Padova, Department of Physics and Astronomy, Via Marzolo 8, 35131 Padova, Italy.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Derzsi
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. garst@ichf.edu.pl.
authors.forename: Tomasz S
authors.initials: TS
authors.lastname: Kaminski
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. garst@ichf.edu.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Garstecki
mesh_terms: D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*