title: QconCAT: Internal Standard for Protein Quantification.
abstract: Protein quantification based on stable isotope labeling-mass spectrometry involves adding known quantities of stable isotope-labeled internal standards into biological samples. The internal standards are analogous to analyte molecules and quantification is achieved by comparing signals from isotope-labeled and analyte molecules. This methodology is broadly applicable to proteomics research, biomarker discovery and validation, and clinical studies, which require accurate and precise protein abundance measurements. One such internal standard platform for protein quantification is concatenated peptides (QconCAT). This chapter describes a protocol for the design, expression, characterization, and application of the QconCAT strategy for protein quantification.
authors.affiliation: Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA. Electronic address: kbauer@ibbr.umd.edu.
authors.forename: Kerry Bauer
authors.initials: KB
authors.lastname: Scott
authors.affiliation: Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
authors.forename: Illarion V
authors.initials: IV
authors.lastname: Turko
authors.affiliation: Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.
authors.forename: Karen W
authors.initials: KW
authors.lastname: Phinney
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D040901:Proteomics
chemical_list: D011506:Proteins
keywords: Mass spectrometry; Protein quantification; Proteomics; Protocol; QconCAT; Stable isotope labeling