title: Overexpression of the S100A2 protein as a prognostic marker for patients with stage II and III colorectal cancer.
abstract: We aimed to identify a novel prognostic biomarker related to recurrence in stage II and III colorectal cancer (CRC) patients. Stage II and III CRC tissue mRNA expression was profiled using an Affymetrix Gene Chip, and copy number profiles of 125 patients were generated using an Affymetrix 250K Sty array. Genes showing both upregulated expression and copy number gains in cases involving recurrence were extracted as candidate biomarkers. The protein expression of the candidate gene was assessed using immunohistochemical staining of tissue from 161 patients. The relationship between protein expression and clinicopathological features was also examined. We identified 9 candidate genes related to recurrence of stage II and III CRC, whose mRNA expression was significantly higher in CRC than in normal tissue. Of these proteins, the S100 calcium-binding protein A2 (S100A2) has been observed in several human cancers. S100A2 protein overexpression in CRC cells was associated with significantly worse overall survival and relapse-free survival, indicating that S100A2 is an independent risk factor for stage II and III CRC recurrence. S100A2 overexpression in cancer cells could be a biomarker of poor prognosis in stage II and III CRC recurrence and a target for treatment of this disease.
authors.affiliation: Department of Surgical Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Masuda
authors.affiliation: Department of Surgical Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: Department of Systems Biology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Mogushi
authors.affiliation: Department of Surgical Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okazaki
authors.affiliation: Department of Translational Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Ishiguro
authors.affiliation: Department of Surgical Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Iida
authors.affiliation: Department of Medical Omics Informatics, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizushima
authors.affiliation: Department of Systems Biology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Surgical Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Uetake
authors.affiliation: Department of Surgical Oncology, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo 113-8510, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Sugihara
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002630:Chemotactic Factors / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D009418:S100 Proteins / Q000378:metabolism*; D016896:Treatment Outcome; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D002630:Chemotactic Factors; D009418:S100 Proteins; C098997:S100A2 protein, human