title: Bacterial reduction of highly concentrated perchlorate: Kinetics and influence of co-existing electron acceptors, temperature, pH and electron donors.
abstract: Perchlorate reduction kinetics and effects of various environmental conditions on removal of perchlorate from synthetic water were investigated to seek high-strength perchlorate removal using mixed perchlorate reducing bacteria. Results demonstrated that perchlorate (50-1500 mg L(-1)) could be degraded rapidly within 28 h under the optimal conditions. The maximum specific perchlorate reduction rate (qmax) and half saturation constant (Ks) were 0.92 mg-perchlorate (mg-dry weight)(-1) h(-1) and 157.7 mg L(-1), respectively. In the ClO4(-)-NO3(-) systems obvious but recoverable lags were caused in perchlorate reduction and the lag time increased with the ratio of nitrate to perchlorate concentration increasing from 0.5 to 3. While in the ClO4(-)-SO4(2-) systems inhibitions didn't occur until the ratio of sulfate to perchlorate concentration exceeded 10. The optimum temperature and pH value were 35 °C and 6.85, respectively. The optimal acetate-to-perchlorate ratio that could consume all perchlorate and acetate simultaneously was about 2. Dechloromonas, one of the most prominent perchlorate reducing bacteria, was identified as the dominant bacterium in the acclimated culture (69.33% of the whole clones). The study demonstrated that the perchlorate-acclimated mixed microorganisms can readily and efficiently realize reduction of highly concentrated perchlorate in wastewater.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China. Electronic address: gaonaiyun@126.com.
authors.forename: Naiyun
authors.initials: N
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors.forename: Shuaifeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Xu
mesh_terms: D020562:Betaproteobacteria / Q000254:growth & development*; D001673:Biodegradation, Environmental; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009566:Nitrates / Q000737:chemistry*; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000032:analysis* / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry*; D013696:Temperature; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D009566:Nitrates; D010472:Perchlorates; D013431:Sulfates; D062065:Wastewater; D014874:Water Pollutants, Chemical; C494474:perchlorate
keywords: Environmental conditions; Highly concentrated; Kinetics; Perchlorate; Perchlorate reducing bacteria (PCRB)