title: Analysis of genetic variation of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Central China.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is an epidemic etiology in pigs of all ages causing reproductive failure and respiratory manifestation. PRRSV has been circulating in Chinese pig farms for almost 20 years. The aim of the present study was to fully understand the extent of the genetic diversity and molecular characteristics of PRRSVs in Central China. A strain of PRRSV isolated from a recent outbreak farm in Hunan province in Central China, designated HUN-2014, was sequenced and analyzed with 39 other PRRSVs from 1998 to 2014 in Central China. Comparative results of genomic sequences revealed that all 40 PRRSVs belonged to the North American genotype (NA genotype) and shared 88.8-99.0% homology. Phylogenetic analysis showed three subgenotypes, namely conventional PRRSV (C-PRRSV), specially mutant PRRSV (S-PRRSV) and highly pathogenic PRRSV (HP-PRRSV), in all 40 PRRSVs. Moreover, comparative analysis of amino acid (AA) sequences of NSP2, GP3, GP5 and ORF5a revealed the main evolution trend of PRRSVs in Central China from 1998 to 2014, which was from C-PRRSV to HP-PRRSV, accompanied by different evolving directions to S-PRRSV. In conclusion, both the major evolutionary trend and special features of genetic variation should be emphasized as theoretical basis for development of new vaccines and control strategies for PRRS.
authors.affiliation: Department of Inspection Technology Research, China Institute of Veterinary Drug Control, Beijing 100081, China.
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.forename: Yibao
authors.initials: Y
authors.lastname: Ning
authors.forename: Binrui
authors.initials: B
authors.lastname: Xu
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Gong
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D004279:DNA, Viral; D019353:Endemic Diseases; D014644:Genetic Variation; D016679:Genome, Viral; D058889:Molecular Typing; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013552:Swine; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins