title: Biocompatible 3D Matrix with Antimicrobial Properties.
abstract: The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL), hydroxyapatite (HAp), β-cyclodextrin (β-CD) and usnic acid (UA). The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Microscopy (FT-IRM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA) against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D) anti-infective regeneration matrix for bone tissue engineering.
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. alberto_ion@yahoo.com.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ion
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. ecaterina.andronescu@upb.ro.
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Andronescu
authors.affiliation: Department of Orthopedics and Traumatology, Bucharest University Hospital, 169 Splaiul Independentei, 050098 Bucharest, Romania. dragosradulescu1988@yahoo.com.
authors.forename: Dragoș
authors.initials: D
authors.lastname: Rădulescu
authors.affiliation: Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. radulescu_marius@yahoo.com.
authors.forename: Marius
authors.initials: M
authors.lastname: Rădulescu
authors.affiliation: Department of Fetal and Adult Stem Cell Therapy, Institute of Cellular Biology and Pathology of Romanian Academy, 8, B.P. Hasdeu, 050568 Bucharest, Romania. floriniordache84@yahoo.com.
authors.forename: Florin
authors.initials: F
authors.lastname: Iordache
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. bogdan.vasile@upb.ro.
authors.forename: Bogdan Ștefan
authors.initials: BȘ
authors.lastname: Vasile
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. adrian_v_surdu@yahoo.com.
authors.forename: Adrian Vasile
authors.initials: AV
authors.lastname: Surdu
authors.affiliation: Department of Collagen, Branch of Leather and Footwear Research, National Institute of Research and Development for Textile and Leather, 93 I. Minulescu Street, 031215 Bucharest, Romania. albu_mada@yahoo.com.
authors.forename: Madalina Georgiana
authors.initials: MG
authors.lastname: Albu
authors.affiliation: Department of Fetal and Adult Stem Cell Therapy, Institute of Cellular Biology and Pathology of Romanian Academy, 8, B.P. Hasdeu, 050568 Bucharest, Romania. horia.maniu@gmail.com.
authors.forename: Horia
authors.initials: H
authors.lastname: Maniu
authors.affiliation: Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania. carmen_balotescu@yahoo.com.|Research Institute of the University of Bucharest, Life, Environmental and Earth Sciences, Spl. Independentei 91-95, 0500088 Bucharest, Romania. carmen_balotescu@yahoo.com.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. grumezescu@yahoo.com.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. alina_m_h@yahoo.com.|Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania. alina_m_h@yahoo.com.
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001572:Benzofurans / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D001572:Benzofurans; D001672:Biocompatible Materials; C073339:usnic acid; D003094:Collagen; D017886:Durapatite
keywords: Gram positive; S. aureus; biocompatible; collagen; cyclodextrins; drug delivery; hydroxyapatite; in vitro; usnic acid