title: Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells.
abstract: Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were cultured in high-glucose Dulbecco's modified Eagle's medium with 10% FBS. Studied cells were subdivided into five groups: treated with scramble, anti-miR-29a, anti-miR-29a + Taxol, Taxol, and control. Taxol was added 24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation of HSP27, HSP40, HSP70, and HSP90 levels and could be contemplated as a compelling alternative for Taxol employment with similar effects and/or to sensitize cancer cells to chemotherapy with fewer side effects.
authors.affiliation: Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
authors.forename: Encieh
authors.initials: E
authors.lastname: Choghaei
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Khamisipour
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Falahati
authors.forename: Behrooz
authors.initials: B
authors.lastname: Naeimi
authors.forename: Majid
authors.initials: M
authors.lastname: Mossahebi-Mohammadi
authors.forename: Rahim
authors.initials: R
authors.lastname: Tahmasebi
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Hasanpour
authors.forename: Shakib
authors.initials: S
authors.lastname: Shamsian
authors.forename: Zahra Sadat
authors.initials: ZS
authors.lastname: Hashemi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology; D034622:RNA Interference
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006360:Heat-Shock Proteins; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D017239:Paclitaxel