title: Disorder of endoplasmic reticulum calcium channel components is associated with the increased apoptotic potential in pale, soft, exudative pork.
abstract: Eight pale, soft and exudative (PSE) and eight reddish-pink, firm and non-exudative (RFN) porcine longissimus muscle samples were selected based on pH and L* at 1h postmortem (PM), and drip loss at 24h PM, and used to evaluate the cellular calcium and apoptosis status. We found that SERCA1 was decreased, while IP3R was decreased in PSE meat (P<0.05), indicative of the overloaded sarcoplasmic calcium status. In PSE meat, the pro-apoptotic factor BAX was increased while the anti-apoptotic factor Bcl-2 was decreased (P<0.05). The significantly increased activity of caspase 3 and the expression of its cleavage fragment suggested higher apoptotic potential in PSE meat compared with RFN meat (P<0.05). Moreover, the significantly higher expression level of cytochrome C (P<0.05) suggests the important role of mitochondria during apoptosis appearance in PSE meat. Taken together, our data inferred that the calcium channel disorder present in PSE meat was associated with the increased apoptotic potential.
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Synergetic Innovation Centre of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Guo
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Synergetic Innovation Centre of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China.
authors.forename: Wangang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: CSIRO Food and Nutrition Flagship, 39 Kessels Road, Queensland 4108, Australia.
authors.forename: Ron K
authors.initials: RK
authors.lastname: Tume
authors.affiliation: CSIRO Agriculture Flagship, 306 Carmody Road, Queensland 4067, Australia.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Hudson
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Synergetic Innovation Centre of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Synergetic Innovation Centre of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China. Electronic address: gh.zhou@njau.edu.cn.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D003116:Color; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D005511:Food Handling; D063427:Food Quality; D006863:Hydrogen-Ion Concentration; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry; D000069466:Red Meat / Q000032:analysis*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D013552:Swine; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D009124:Muscle Proteins; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D053148:Caspase 3; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium
keywords: Apoptosis; Calcium imbalance; Mitochondrial; PSE meat