title: MALDI imaging mass spectrometry as a novel tool for detecting histone modifications in clinical tissue samples.
abstract: Histone post-translational modifications (PTMs), histone variants and enzymes responsible for the incorporation or the removal of the PTMs are being increasingly associated with human disease. Combinations of histone PTMs and the specific incorporation of variants contribute to the establishment of cellular identity and hence are potential markers that could be exploited in disease diagnostics and prognostics and therapy response prediction. Due to the scarcity of suitable antibodies and the pre-requirement of tissue homogenization for more advanced analytical techniques, comprehensive information regarding the spatial distribution of these factors at the tissue level has been lacking. MALDI imaging mass spectrometry provides an ideal platform to measure histone PTMs and variants from tissues while maintaining the information about their spatial distribution. Discussed in this review are the relevance of histones in the context of human disease and the contribution of MALDI imaging mass spectrometry in measuring histones in situ.
authors.affiliation: a Protein Analysis Unit (ZfP), Biomedical Center (BMC) , Ludwig Maximilians University Munich , Planegg-Martinsried , Germany.
authors.forename: Shibojyoti
authors.initials: S
authors.lastname: Lahiri
authors.affiliation: b Research Unit Analytical Pathology , Helmholtz Zentrum München, German Research Center for Environmental Health , Neuherberg , Germany.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: b Research Unit Analytical Pathology , Helmholtz Zentrum München, German Research Center for Environmental Health , Neuherberg , Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Buck
authors.affiliation: a Protein Analysis Unit (ZfP), Biomedical Center (BMC) , Ludwig Maximilians University Munich , Planegg-Martinsried , Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.affiliation: b Research Unit Analytical Pathology , Helmholtz Zentrum München, German Research Center for Environmental Health , Neuherberg , Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Walch
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D006657:Histones
keywords: Histone modifications; MALDI-IMS; clinical tissue samples; disease markers; nuclear density