title: Enhanced biocatalysis mechanism under microwave irradiation in isoquercitrin production revealed by circular dichroism and surface plasmon resonance spectroscopy.
abstract: An efficient and rapid process for isoquercitrin production by hesperidinase-catalyzed hydrolysis of rutin was successfully developed under microwave irradiation detecting the affinity by circular dichroism (CD) and surface plasmon resonance (SPR) spectroscopy. A maximum isoquercitrin yield of 91.5±2.7% was obtained in 10min with the conditions of 10g/L hesperidinase, 2g/L rutin, 30°C and microwave power density 88.9W/L. Enzymatic reaction rate and Vm/Km in the microwave reactor were 6.34-fold higher than in a continuous flow microreactor and 1.24-fold higher than in a biphasic system. CD and SPR analysis results also showed that hesperidinase has a better selectivity and affinity (3.3-fold than in a batch reactor) to generate isoquercitrin under microwave irradiation. Microwave irradiation greatly improved the reaction efficiency and productivity, leading to a more positive economical assessment. The binding affinity indicates the presence of strong multivalent interactions between rutin and hesperidinase under microwave irradiation.
authors.affiliation: School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: An
authors.initials: A
authors.lastname: Gong
authors.affiliation: School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Yi-Yuan
authors.initials: YY
authors.lastname: Mei
authors.affiliation: School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xu
authors.affiliation: School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China.
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Wu
authors.affiliation: School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China. Electronic address: wangjun@just.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D055162:Biocatalysis; D002384:Catalysis; D002942:Circular Dichroism; D006026:Glycoside Hydrolases / Q000378:metabolism* / Q000528:radiation effects; D006868:Hydrolysis; D008872:Microwaves; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism; D012431:Rutin / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013696:Temperature
chemical_list: C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin; D006026:Glycoside Hydrolases; C023307:hesperidinase
keywords: Circular dichroism; Enzymatic biocatalysis; Isoquercitrin; Microwave irradiation; Surface plasmon resonance