title: Long intergenic non-coding RNA 00152 promotes tumor cell cycle progression by binding to EZH2 and repressing p15 and p21 in gastric cancer.
abstract: Long noncoding RNAs (lncRNAs) play important regulatory roles in several human cancers. Integrated analysis revealed that expression of long intergenic non-coding RNA 152 (LINC00152) was significantly upregulated in gastric cancer (GC). Further analysis in a cohort of 97 GC patients revealed that LINC00152 expression was positively correlated with tumor invasion depth, lymph node metastasis, higher TNM stage, and poor survival. Gene set enrichment analysis revealed that cell proliferation and cell cycle progression were increased in patients with high LINC00152 expression. In both GC cell lines and xenograft systems, LINC00152 overexpression facilitated GC cell proliferation by accelerating the cell cycle, whereas LINC00152 knockdown had the opposite effect. Moreover, by binding to enhancer of zeste homolog 2 (EZH2), LINC00152 promotes GC tumor cell cycle progression by silencing the expression of p15 and p21. These findings suggest that LINC00152 may play contribute to the progression of GC and may be an effective therapeutic target.
authors.affiliation: Department of Oncology, Jining NO.1 People's Hospital, Jining City, Shandong Province 272011, China.
authors.forename: Wen-ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Jiangsu Province 223300, China.
authors.forename: Ming-de
authors.initials: MD
authors.lastname: Huang
authors.affiliation: Department of Oncology, Jining NO.1 People's Hospital, Jining City, Shandong Province 272011, China.
authors.forename: Dao-ping
authors.initials: DP
authors.lastname: Sun
authors.affiliation: Central Laboratory, Subei People's Hospital of Jiangsu province, Yangzhou, Jiangsu Province 225001, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Kong
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, Jiangsu Province 210029, China.
authors.forename: Tong-peng
authors.initials: TP
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xia
authors.affiliation: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.
authors.forename: Er-bao
authors.initials: EB
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, Jiangsu Province 210029, China.
authors.forename: Yong-qian
authors.initials: YQ
authors.lastname: Shu
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000037:antagonists & inhibitors* / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000037:antagonists & inhibitors* / Q000235:genetics; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000378:metabolism*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*
chemical_list: D050762:Cyclin-Dependent Kinase Inhibitor p15; D050759:Cyclin-Dependent Kinase Inhibitor p21; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; C000608524:long non-coding RNA Linc00152, human; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein
keywords: LINC00152; cell proliferation; gastric cancer; long non-coding RNA; p15/p21