title: A thermostable Gloeophyllum trabeum xylanase with potential for the brewing industry.
abstract: A xylanase gene of glycoside hydrolase family 10, GtXyn10, was cloned from Gloeophyllum trabeum CBS 900.73 and expressed in Pichia pastoris GS115. Purified recombinant GtXyn10 exhibited significant activities to xylan (100.0%), lichenan (11.2%), glucan (15.2%) and p-nitrophenol-β-cellobiose (18.6%), demonstrated the maximum xylanase and glucanase activities at pH 4.5-5.0 and 75°C, retained stability over the pH range of 2.0-7.5 and at 70°C, and was resistant to pepsin and trypsin, most metal ions and SDS. Multiple sequence alignment and modeled-structure analysis identified a unique Gly48 in GtXyn10, and site-directed mutagenesis of Gly48 to Lys improved the temperature optimum up to 80°C. Under simulated mashing conditions, GtXyn10 (80U) reduced the mash viscosity by 12.8% and improved the filtration rate by 31.3%. All these properties above make GtXyn10 attractive for potential applications in the feed and brewing industries.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.affiliation: Medical College, Hebei University of Engineering, Handan, Hebei 056038, People's Republic of China.
authors.forename: Wangning
authors.initials: W
authors.lastname: Yu
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: You
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China.
authors.forename: Weina
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Hou
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zheng
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China. Electronic address: xxm1005@126.com.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Xie
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China. Electronic address: binyao@caas.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D003001:Cloning, Molecular; D004063:Digestion; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D005285:Fermentation; D007218:Industrial Microbiology; D010843:Pichia / Q000235:genetics
chemical_list: D043364:Endo-1,4-beta Xylanases
keywords: Gloeophyllum trabeum; Glycoside hydrolase family 10 (GH10); Thermo-tolerant; Xylanase