title: Combined 1,25-Dihydroxy-vitamin D and Resveratrol: A Novel Therapeutic Approach to Ameliorate Ischemia Reperfusion-Induced Myocardial Injury.
abstract: The aim of this study was to assess the effect of combined 1,25-dihydroxyvitamin D (1,25 D) and resveratrol on cardiac arrhythmias, infarct size, and transcription of catalase, thioredoxin-1 and B-cell lymphoma 2 (Bcl-2), following myocardial ischemia-reperfusion (IR) in male rats. Ligation of coronary artery was performed in rats (n = 6 per group) without any treatment (IR group), pretreated with 0.1 μg/kg/day of 1,25 D (1,25 D + IR), 1 mg/kg/day of resveratrol (Res + IR) or a combination (1,25 D + Res + IR) for 14 days. Arrhythmias were analyzed according to the Lambeth conventions, and infarct size was measured by 2,3,5-triphenyl-2H-tetrazolium chloride staining. Expression of prosurvival genes was evaluated by real-time polymerase chain reaction. In the 1,25 D + Res + IR group the mean infarct size was 17.6 ± 3.5 %, which was significantly less than that in the IR, 1,25 D + IR, and Res + IR groups (p < 0.001). Although the single therapy of either 1,25 D or resveratrol did not change the incidence of arrhythmias significantly, a reduction in the number of ventricular ectopic beats was noted in group 1,25 D + Res + IR (179.19 ± 58.87, p < 0.001 vs IR; p < 0.05 vs Res + IR; p < 0.01 vs Vit D + IR). Combination of 1,25 D and resveratrol increased transcription of catalase by 119 ± 37 % (p < 0.001 vs IR, p < 0.01 vs Res + IR, p < 0.001 vs 1,25 D + IR). Our study showed that combination of a non-hypotensive dose of 1,25 D and resveratrol can be a novel and effective strategy for protecting against ischemia.
authors.affiliation: 1 Herbal Medicine Research Center, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.|2 Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Safari
authors.affiliation: 3 Department of Physiology, International Campus, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Zarei
authors.affiliation: 4 Department of Physiology, Arsanjan Branch, Islamic Azad University, Fars, Iran.
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Shekarforoush
authors.affiliation: 5 , Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
authors.forename: Asefeh
authors.initials: A
authors.lastname: Fekri
authors.affiliation: 2 Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
authors.forename: Mohsen Sharifi
authors.initials: MS
authors.lastname: Klishadi
authors.affiliation: 6 Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
authors.forename: Seyedhossein
authors.initials: S
authors.lastname: Hekmatimoghaddam
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D001145:Arrhythmias, Cardiac; D002118:Calcium / Q000097:blood; D004359:Drug Therapy, Combination; D008297:Male; D009203:Myocardial Infarction / Q000150:complications / Q000209:etiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000517:prevention & control*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D014807:Vitamin D / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D012333:RNA, Messenger; D013267:Stilbenes; D014815:Vitamins; D014807:Vitamin D; C097949:1,25-dihydroxyvitamin D; D000077185:Resveratrol; D002118:Calcium
keywords: 1,25-Dihydroxyvitamin D; catalase; myocardial ischemia-reperfusion; resveratrol