title: Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris.
abstract: Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and the enhanced biological activity of myostatin propeptide mutant. Modified myostatin propeptide gene was cloned into the pPIC9K plasmid to form the recombinant plasmid pPIC9K-Msp. Recombinant plasmid pPIC9K-Msp was transformed into Pichia pastoris GS115 by electrotransformation. Transformed cells were screened, and methanol was used to induce expression. SDS-PAGE and western blotting were used to verify the successful expression of myostatin propeptide with biological activity in Pichia pastoris, providing the basis for characterization of this protein.
authors.affiliation: College of Animal Science, Shihezi University, Shihezi, China.|Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: W
authors.initials: W
authors.lastname: Du
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: J
authors.initials: J
authors.lastname: Xia
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Shihezi University, Shihezi, China.|Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: M J
authors.initials: MJ
authors.lastname: Liu
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Li
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Yan
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Xie
mesh_terms: D015870:Gene Expression; D055435:Myostatin / Q000235:genetics* / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D055435:Myostatin; D011994:Recombinant Proteins