title: Emulsions Made of Oils from Seeds of GM Flax Protect V79 Cells against Oxidative Stress.
abstract: Polyunsaturated fatty acids, sterols, and hydrophilic phenolic compounds are components of flax oil that act as antioxidants. We investigated the impact of flax oil from transgenic flax in the form of emulsions on stressed Chinese hamster pulmonary fibroblasts. We found that the emulsions protect V79 cells against the H2O2 and the effect is dose dependent. They reduced the level of intracellular reactive oxygen species and protected genomic DNA against damage. The rate of cell proliferation increased upon treatment with the emulsions at a low concentration, while at a high concentration it decreased significantly, accompanied by increased frequency of apoptotic cell death. Expression analysis of selected genes revealed the upregulatory impact of the emulsions on the histones, acetylases, and deacetylases. Expression of apoptotic, proinflammatory, and anti-inflammatory genes was also altered. It is thus suggested that flax oil emulsions might be useful as a basis for biomedical products that actively protect cells against inflammation and degeneration. The beneficial effect on fibroblast resistance to oxidative damage was superior in the emulsion made of oil from transgenic plants which was correlated with the quantity of antioxidants and squalene. The emulsions from transgenic flax are promising candidates for skin protection against oxidative damage.
authors.affiliation: Department of Endocrinology, 4th Clinical Military Hospital, Rudolfa Weigla 5, 50-981 Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Skorkowska-Telichowska
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Hasiewicz-Derkacz
authors.affiliation: Department of Basic Medical Sciences, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gębarowski
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kulma
authors.affiliation: Department of Basic Medical Sciences, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.
authors.forename: Helena
authors.identifier: 0000-0002-8084-3686
authors.initials: H
authors.lastname: Moreira
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kostyn
authors.affiliation: Department of Basic Medical Sciences, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gębczak
authors.affiliation: Department of Basic Medical Sciences, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Szyjka
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
authors.forename: Wioleta
authors.initials: W
authors.lastname: Wojtasik
authors.affiliation: Department of Basic Medical Sciences, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Gąsiorowski
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D019610:Cytoprotection / Q000187:drug effects*; D004247:DNA / Q000378:metabolism; D004655:Emulsions; D019597:Flax / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D016678:Genome; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D018709:Statistics, Nonparametric
chemical_list: D000975:Antioxidants; D004655:Emulsions; D010938:Plant Oils; D017382:Reactive Oxygen Species; D004247:DNA