title: Total Antioxidant Capacity and Total Oxidant Status in Saliva of Periodontitis Patients in Relation to Bacterial Load.
abstract: The detection of salivary biomarkers has a potential application in early diagnosis and monitoring of periodontal inflammation. However, searching sensitive salivary biomarkers for periodontitis is still ongoing. Oxidative stress is supposed to play an important role in periodontitis progression and tissue destruction. In this cross-sectional study, we investigated total antioxidant capacity (TAC) and total oxidant status (TOS) in saliva of periodontitis patients compared to healthy controls and their relationship with periodontopathic bacteria and periodontal disease severity. Unstimulated saliva was collected from 45 patients with generalized severe periodontitis and 37 healthy individuals and the TAC/TOS were measured. In addition, salivary levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Fusobacterium nucleatum in saliva were measured. Salivary TAC was lower in periodontitis patients compared to healthy controls. Moreover, a significant negative correlation of salivary TAC with clinical attachment loss was observed in periodontitis patients. No significant difference in the salivary TOS was observed between periodontitis patients and healthy controls. Bacterial load was enhanced in periodontitis patients and exhibited correlation with periodontal disease severity but not with salivary TAC/TOS. Our data suggest that changes in antioxidant capacity in periodontitis patients are not associated with increased bacterial load and are probably due to a dysregulated immune response.
authors.affiliation: Department of Science and Education, Yantai Stomatological Hospital, Binzhou Medical UniversityYantai, China; Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of ViennaVienna, Austria.
authors.forename: Taowen
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna Vienna, Austria.
authors.forename: Oleh
authors.initials: O
authors.lastname: Andrukhov
authors.affiliation: Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna Vienna, Austria.
authors.forename: Hady
authors.initials: H
authors.lastname: Haririan
authors.affiliation: Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Müller-Kern
authors.affiliation: Department of Science and Education, Yantai Stomatological Hospital, Binzhou Medical University Yantai, China.
authors.forename: Shutai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Science and Education, Yantai Stomatological Hospital, Binzhou Medical University Yantai, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna Vienna, Austria.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Rausch-Fan
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001419:Bacteria / Q000302:isolation & purification; D058491:Bacterial Load; D003430:Cross-Sectional Studies; D006801:Humans; D016877:Oxidants; D010518:Periodontitis / Q000382:microbiology* / Q000473:pathology*; D012463:Saliva / Q000737:chemistry* / Q000382:microbiology*
chemical_list: D000975:Antioxidants; D016877:Oxidants
keywords: oxidative stress; periodontopathic bacteria; saliva; total antioxidant capacity; total oxidant status