title: The first genetic map of a synthesized allohexaploid Brassica with A, B and C genomes based on simple sequence repeat markers.
abstract: We present the first genetic map of an allohexaploid Brassica species, based on segregating microsatellite markers in a doubled haploid mapping population generated from a hybrid between two hexaploid parents. This study reports the first genetic map of trigenomic Brassica. A doubled haploid mapping population consisting of 189 lines was obtained via microspore culture from a hybrid H16-1 derived from a cross between two allohexaploid Brassica lines (7H170-1 and Y54-2). Simple sequence repeat primer pairs specific to the A genome (107), B genome (44) and C genome (109) were used to construct a genetic linkage map of the population. Twenty-seven linkage groups were resolved from 274 polymorphic loci on the A genome (109), B genome (49) and C genome (116) covering a total genetic distance of 3178.8 cM with an average distance between markers of 11.60 cM. This is the first genetic framework map for the artificially synthesized Brassica allohexaploids. The linkage groups represent the expected complement of chromosomes in the A, B and C genomes from the original diploid and tetraploid parents. This framework linkage map will be valuable for QTL analysis and future genetic improvement of a new allohexaploid Brassica species, and in improving our understanding of the genetic control of meiosis in new polyploids.
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China.|The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.affiliation: School of Plant Biology, The University of Western Australia, Perth, WA, 6009, Australia.|The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China.|The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.|Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Geng
authors.affiliation: School of Plant Biology, The University of Western Australia, Perth, WA, 6009, Australia.|The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Meng
authors.affiliation: The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia. wallace.cowling@uwa.edu.au.
authors.forename: W A
authors.initials: WA
authors.lastname: Cowling
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China. wjzhou@zju.edu.cn.
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhou
mesh_terms: D001937:Brassica / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D006238:Haploidy; D018895:Microsatellite Repeats; D011123:Polyploidy
chemical_list: D017931:DNA Primers; D018744:DNA, Plant