title: Quantitative and fingerprinting analysis of Pogostemon cablin based on GC-FID combined with chemometrics.
abstract: In this study, a simple, sensitive and reliable gas chromatography-flame ionization detection (GC-FID) method is established for quantitative chemical fingerprinting of essential oils from Pogostemon cablin. Oil samples are prepared by hydrodistillation, with yields ranging from 0.73% to 2.02%. The two main components of the oil, patchouli alcohol and pogostone, were detected simultaneously in 36 samples and were found to have average contents of 43.07% and 7.84%, respectively. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. All calibration curves showed excellent linearity (r(2)>0.9992) within the test ranges, and the relative standard deviation (RSD) values for intra- and inter-day precision were less than 1.5%, indicating a high degree of precision. The GC-FID chemical fingerprints of the 36 samples were established using 12 common peaks which account for over 90% of the total peak area. Chemometric techniques, including similarity analysis and hierarchical cluster analysis, were also employed to explore the similarities and outstanding consistencies among different P. cablin oil samples. The results demonstrate that chromatographic fingerprinting and quantitative analysis can be achieved simultaneously when evaluating quality and authenticating samples of P. cablin.
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang 330013, PR China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China.
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China; Shanghai Institute of Technology, Shanghai 201400, PR China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Feng
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Dou
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, PR China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang 330013, PR China. Electronic address: xiaoqiang@tsinghua.org.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. Electronic address: yangmeihua15@hotmail.com.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D005410:Flame Ionization / Q000379:methods*; D009822:Oils, Volatile / Q000737:chemistry; D000070177:Pogostemon / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry
chemical_list: D009822:Oils, Volatile; C483658:Pogostone; D012717:Sesquiterpenes; C052983:patchouli alcohol
keywords: Chemometrics; Fingerprinting analysis; GC-FID; Pogostemon cablin; Quality control; Quantitative analysis