title: Different effects of H2O2 treatment on cervical squamous carcinoma cells and adenocarcinoma cells.
abstract: BACKGROUND
  This study aims to compare the antioxidant abilities of cervical squamous carcinoma cells and cervical adenocarcinoma cells and to study the related mechanisms.
  
  
  METHODS
  Cervical squamous carcinoma and adenocarcinoma cells were treated with H2O2. Cell proliferation was determined with the MTT assay. The reactive oxygen species (ROS) level was detected by the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) method. The 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) method was performed to measure intracellular concentrations of reduced glutathione (GSH) and oxidized glutathione (GSSG). The nitrite formation method, the molybdate colorimetric method, and the DTNB colorimetric method were used to determine activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), respectively.
  
  
  RESULTS
  Compared with untreated control cells, cell proliferation of cervical squamous carcinoma cells and cervical adenocarcinoma cells was significantly inhibited by H2O2 treatment (p < 0.05). Reactive oxygen species levels and GSSG levels were significantly increased (p < 0.01), whereas GSH levels were significantly decreased (p < 0.05 or 0.01) in both cells after H2O2 treatment. Thus the ratio of GSH/GSSG was significantly decreased by H2O2 treatment in both cells (p < 0.01). In addition, H2O2 treatment significantly increased activities of SOD, CAT, and GPx in both cells (p < 0.05 or 0.01). Furthermore, the above-mentioned changes induced by H2O2 treatment were more dramatic in cervical squamous carcinoma cells.
  
  
  CONCLUSIONS
  The antioxidant ability of cervical squamous carcinoma cells is lower than that of cervical adenocarcinoma cells, which may be related to the increased ROS levels in cervical squamous carcinoma cells induced by H2O2 treatments.
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Peihai
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Jinan Central Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Obstetrics and Gynecology, Jinan Central Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Sie
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan, Shandong, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Peng
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan, Shandong, China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Bi
authors.affiliation: Department of Gynecology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
keywords: antioxidative enzyme; cervical neoplasm; glutathione; hydrogen dioxide; reactive oxygen species