title: Regulation of cuticle formation during fruit development and ripening in 'Newhall' navel orange (Citrus sinensis Osbeck) revealed by transcriptomic and metabolomic profiling.
abstract: Fruit cuticle, which is composed of cutin and wax and biosynthesized during fruit development, plays important roles in the prevention of water loss and the resistance to pathogen infection during fruit development and postharvest storage. However, the key factors and mechanisms regarding the cuticle biosynthesis in citrus fruits are still unclear. Here, fruit cuticle of 'Newhall' navel orange (Citrus sinensis Osbeck) was studied from the stage of fruit expansion to postharvest storage from the perspectives of morphology, transcription and metabolism. The results demonstrated that cutin accumulation is synchronous with fruit expansion, while wax synthesis is synchronous with fruit maturation. Metabolic profile of fruits peel revealed that transition of metabolism of fruit peel occurred from 120 to 150 DAF and ABA was predicted to regulate citrus wax synthesis during the development of Newhall fruits. RNA-seq analysis of the peel from the above two stages manifested that the genes involved in photosynthesis were repressed, while the genes involved in the biosynthesis of wax, cutin and lignin were significantly induced at later stages. Further real-time PCR predicted that MYB transcription factor GL1-like regulates citrus fruits wax synthesis. These results are valuable for improving the fruit quality during development and storage.
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: shiyuejq023@163.com.
authors.forename: Jinqiu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: xiaomienjoy2008@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: shelleydatou@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: he15983535117@126.com.
authors.forename: Yizhong
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: luotao0502@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: 596815970@qq.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: 838782544@qq.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: zengyl@mail.hzau.edu.cn.
authors.forename: Yunliu
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: xujuan@mail.hzau.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: xxdeng@mail.hzau.edu.cn.
authors.forename: Xiuxin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: yjcheng@mail.hzau.edu.cn.
authors.forename: Yunjiang
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D032084:Citrus sinensis / Q000254:growth & development*; D005638:Fruit / Q000254:growth & development; D055442:Metabolome; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D059467:Transcriptome
keywords: ABA; Citrus fruit; Cutin; Fruit development; Metabolic profiling; Transcript analysis; Waxes