title: Exploiting eco-physiological niche to facilitate the separation of the freshwater cyanobacteria Microcystis sp. and Synechococcus sp.
abstract: In a novel approach to separate the co-occurring freshwater cyanobacteria Microcystis and Synechoccous, published ecological characteristics are used to manipulate temperature and nutrient concentrations to successfully establish a unialgal Microcystis strain. The simple protocol has implications for future cyanobacterial culturing approaches and the establishment of new cyanobacteria strains.
authors.affiliation: Bournemouth University, Centre for Ecology, Environment and Sustainability, Department of Life and Environmental Sciences, Faculty of Science and Technology, Dorset BH12 5BB, UK; Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Rd, The Nothe, Weymouth, Dorset DT4 8UB, UK. Electronic address: dhartnell@bournemouth.ac.uk.
authors.forename: David M
authors.initials: DM
authors.lastname: Hartnell
authors.affiliation: Bournemouth University, Centre for Ecology, Environment and Sustainability, Department of Life and Environmental Sciences, Faculty of Science and Technology, Dorset BH12 5BB, UK.
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Chapman
authors.affiliation: Bournemouth University, Centre for Ecology, Environment and Sustainability, Department of Life and Environmental Sciences, Faculty of Science and Technology, Dorset BH12 5BB, UK.
authors.forename: Genoveva F
authors.initials: GF
authors.lastname: Esteban
authors.affiliation: Bournemouth University, Centre for Ecology, Environment and Sustainability, Department of Life and Environmental Sciences, Faculty of Science and Technology, Dorset BH12 5BB, UK.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Franklin
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D018533:Biomass; D017077:Culture Media, Conditioned; D005434:Flow Cytometry / Q000379:methods; D005502:Food; D005618:Fresh Water / Q000382:microbiology*; D056127:Hydrobiology; D046931:Microcystis / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008856:Microscopy, Fluorescence / Q000379:methods; D046940:Synechococcus / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D017077:Culture Media, Conditioned
keywords: Cyanobacteria; Isolation; Microcystis; Purification; Synechococcus