title: Upregulation of the double-stranded RNA binding protein DGCR8 in invasive ductal breast carcinoma.
abstract: High-throughput experimental studies have indicated that the miRNAome is globally downregulated in various types of malignancy, and dysregulation of miRNAs processing component(s) is one possible mechanism for this phenomenon. Despite the progression in identifying cellular functions of Digeorge Syndrome Critical Region 8 (DGCR8) in miRNAs biogenesis, the role of altered expression of DGCR8 in the pathogenesis of invasive ductal breast carcinoma (IDC) has not yet been fully investigated. The objective of the present study was to evaluate DGCR8 mRNA expression in seventy fresh invasive ductal breast carcinomas and matched adjacent non-neoplastic tissues using quantitative real-time PCR and to assess the value of clinicopathological parameters on its expression. Our findings revealed that DGCR8 mRNA expression is upregulated in more than two-thirds of the cancerous specimens (68.66%) when compared to adjacent non-neoplastic tissue. This difference is statistically significant (P<0.05). We found that DGCR8 mRNA levels were increased in the high-grade and metastatic compared with those of both low-grade and non-metastatic. We demonstrated that there is not significant correlation between DGCR8 mRNA expression levels and clinicopathological parameters. In conclusion, our study suggested that upregulation of DGCR8 may be involved in tumorigenesis and aggressiveness of IDC and may serve as future therapeutic target.
authors.affiliation: Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
authors.forename: Hedieh
authors.initials: H
authors.lastname: Fardmanesh
authors.affiliation: Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. Electronic address: mshekari_ch@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shekari
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.affiliation: Medical Laboratory Science Department, Midwifery-Nursing Institute, Islamic Azad University of Chalous Branch, Chalous, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Alizadeh Shargh
authors.affiliation: Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Poursadegh Zonouzi
authors.affiliation: Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Shakerizadeh
authors.affiliation: Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Poursadegh Zonouzi
authors.affiliation: Department of Biology ,Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Hosseinzadeh
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D016601:RNA-Binding Proteins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C474043:DGCR8 protein, human; D016601:RNA-Binding Proteins
keywords: DGCR8; Invasive Ductal Breast carcinoma; MicroRNA; MicroRNA processing pathway