title: α-Lipoic acid inhibits sevoflurane-induced neuronal apoptosis through PI3K/Akt signalling pathway.
abstract: Sevoflurane is a widely used anaesthetic agent, including in anaesthesia of children and infants. Recent studies indicated that the general anaesthesia might cause the cell apoptosis in the brain. This issue raises the concerns about the neuronal toxicity induced by the application of anaesthetic agents, especially in the infants and young children. In this study, we used Morris water maze, western blotting and immunohistochemistry to elucidate the role of α-lipoic acid in the inhibition of neuronal apoptosis. We found that sevoflurane led to the long-term cognitive impairment in the young rats. This adverse effect may be caused by the neuronal death in the hippocampal region, mediated through PI3K/Akt signalling pathway. We also showed that α-lipoic acid offset the effect of sevoflurane on the neuronal apoptosis and cognitive dysfunction. This study elucidated the potential clinical role of α-lipoic acid, providing a promising way in the prevention and treatment of long-term cognitive impairment induced by sevoflurane general anesthesia.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University (Jiangsu Province Hospital), Nanjing, 210029, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.affiliation: Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University (Jiangsu Province Hospital), Nanjing, 210029, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Peng
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University (Jiangsu Province Hospital), Nanjing, 210029, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University (Jiangsu Province Hospital), Nanjing, 210029, China.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University (Jiangsu Province Hospital), Nanjing, 210029, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University (Jiangsu Province Hospital), Nanjing, 210029, China.
authors.forename: Zhengnian
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000777:Anesthetics / Q000009:adverse effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D008738:Methyl Ethers / Q000009:adverse effects*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D015398:Signal Transduction / Q000187:drug effects*; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D000777:Anesthetics; D008738:Methyl Ethers; D018696:Neuroprotective Agents; D000077149:Sevoflurane; D008063:Thioctic Acid; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: PI3K/Akt signalling pathway; apoptosis; caspase-3; sevoflurane; α-lipoic acid