title: Integrated Magneto-Electrochemical Sensor for Exosome Analysis.
abstract: Extracellular vesicles, including exosomes, are nanoscale membrane particles that carry molecular information on parental cells. They are being pursued as biomarkers of cancers that are difficult to detect or serially follow. Here we present a compact sensor technology for rapid, on-site exosome screening. The sensor is based on an integrated magneto-electrochemical assay: exosomes are immunomagnetically captured from patient samples and profiled through electrochemical reaction. By combining magnetic enrichment and enzymatic amplification, the approach enables (i) highly sensitive, cell-specific exosome detection and (ii) sensor miniaturization and scale-up for high-throughput measurements. As a proof-of-concept, we implemented a portable, eight-channel device and applied it to screen extracellular vesicles in plasma samples from ovarian cancer patients. The sensor allowed for the simultaneous profiling of multiple protein markers within an hour, outperforming conventional methods in assay sensitivity and speed.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.
authors.forename: Sangmoo
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Park
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.
authors.forename: Divya
authors.initials: D
authors.lastname: Pathania
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.|Massachusetts General Hospital Cancer Center , Boston, Massachusetts 02114, United States.
authors.forename: Cesar M
authors.initials: CM
authors.lastname: Castro
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.|Massachusetts General Hospital Cancer Center , Boston, Massachusetts 02114, United States.|Department of Systems Biology, Harvard Medical School , 200 Longwood Avenue, Boston, Massachusetts 02115, United States.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D055354:Exosomes / Q000737:chemistry*; D006801:Humans; D059346:Magnets / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis; D000067716:Point-of-Care Testing
keywords: cancer; diagnostics; electrochemical sensing; extracellular vesicles; point-of-care