title: Antigen-free bovine cancellous bone loaded with recombinant human bone morphogenetic protein-2 for the repair of tibial bone defects in goat model.
abstract: Antigen-free bovine cancellous bone has good performances of porous network structures and mechanics with antigen extracted. To develop a bioactive scaffold for enhancing bone repair and evaluate its biological property, rhBMP-2 loaded with antigen-free bovine cancellous bone was used to treat tibial bone defect. Twenty-four healthy adult goats were chosen to establish goat defects model and randomly divided into four groups. The goats were treated with rhBMP-2/antigen-free bovine cancellous bone scaffolds (group A), autogenous cancellous bone graft (group B), porous tricalciumphosphate scaffolds (group C) and nothing (group D). Animals were evaluated with radiological and histological methods at 4, 8 and 12 weeks after surgery. The gray value of radiographs was used to evaluate the healing of the defects, which revealed that the group A had a better outcome of defect healing compared with group C at 4, 8 and 12 weeks, respectively (p < 0.05), while the difference between groups A and B was without significance at each time (p > 0.05). The newly formed bone area was calculated from histological sections, and the results indicated that the amount of new bone in group A increased significantly compared with that in group C (p < 0.05) but was similar to that in group B (p > 0.05) at 4, 8 and 12 weeks, respectively. In addition, the expression of collagen I and vascular endothelial growth factor by real-time polymerase chain reaction at 12 weeks in group A was significantly higher than that in group C (p = 0.034, p = 0.032, respectively), but no significant differences were found when compared with that in group B (p = 0.36, p = 0.54, respectively). At the same time, group C presented better results than group D on bone defects healing. Therefore, the composites of antigen-free bovine cancellous bone loaded with rhBMP-2 have a good osteoinductive activity and capacity to promote the repair of bone defects.
authors.affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Donghai
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Orthopaedics of Cheng Ban hospital, the Branch of West China Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Zhouyuan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, People's Republic of China kangpd@163.com.
authors.forename: Pengde
authors.initials: P
authors.lastname: Kang
authors.affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tan
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000737:chemistry*; D016025:Bone Transplantation / Q000379:methods; D002130:Calcium Phosphates / Q000737:chemistry; D000071556:Cancellous Bone / Q000276:immunology / Q000637:transplantation*; D002417:Cattle; D003094:Collagen / Q000235:genetics; D005260:Female; D017102:Fracture Healing / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006041:Goats; D006801:Humans; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D013977:Tibia / Q000187:drug effects / Q000502:physiology* / Q000601:surgery* / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000627:therapeutic use*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D000941:Antigens; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen; C018392:tricalcium phosphate
keywords: RhBMP-2; bone defect; bovine cancellous; goat; osteogenesis