title: Maternal and paternal genetic diversity of ancient sheep in Estonia from the Late Bronze Age to the post-medieval period and comparison with other regions in Eurasia.
abstract: Sheep were among the first domesticated animals to appear in Estonia in the late Neolithic and became one of the most widespread livestock species in the region from the Late Bronze Age onwards. However, the origin and historical expansion of local sheep populations in Estonia remain poorly understood. Here, we analysed fragments of the hypervariable D-loop of mitochondrial DNA (mtDNA; 213 bp) and the Y-chromosome SRY gene (130 bp) extracted from 31 archaeological sheep bones dated from approximately 800 BC to 1700 AD. The ancient DNA data of sheep from Estonia were compared with ancient sheep from Finland as well as a set of contemporary sheep breeds from across Eurasia in order to place them in a wider phylogeographical context. The analysis shows that: (i) 24 successfully amplified and analysed mtDNA sequences of ancient sheep cluster into two haplogroups, A and B, of which B is predominant; (ii) four of the ancient mtDNA haplotypes are novel; (iii) higher mtDNA haplotype diversity occurred during the Middle Ages as compared to other periods, a fact concordant with the historical context of expanding international trade during the Middle Ages; (iv) the proportion of rarer haplotypes declined during the expansion of sheep from the Near Eastern domestication centre to the northern European region; (v) three male samples showed the presence of the characteristic northern European haplotype, SNP G-oY1 of the Y-chromosome, and represent the earliest occurrence of this haplotype. Our results provide the first insight into the genetic diversity and phylogeographical background of ancient sheep in Estonia and provide basis for further studies on the temporal fluctuations of ancient sheep populations.
authors.affiliation: Department of Archaeology, Institute of History and Archaeology, University of Tartu, Jakobi 2, 51014, Tartu, Estonia.
authors.forename: E
authors.initials: E
authors.lastname: Rannamäe
authors.affiliation: Department of Archaeobiology, Institute of History, Tallinn University, Rüütli 6, 10130, Tallinn, Estonia.
authors.forename: L
authors.initials: L
authors.lastname: Lõugas
authors.affiliation: Department of Forensic Medicine, University of Helsinki, FI-00014, Helsinki, Finland.|Green Technology, Natural Resources Institute Finland, Myllytie 1, FI-31600, Jokioinen, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Niemi
authors.affiliation: Green Technology, Natural Resources Institute Finland, Myllytie 1, FI-31600, Jokioinen, Finland.|Department of Biology, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Kantanen
authors.affiliation: Department of Archaeobiology, Institute of History, Tallinn University, Rüütli 6, 10130, Tallinn, Estonia.
authors.forename: L
authors.initials: L
authors.lastname: Maldre
authors.affiliation: Department of Natural Sciences, Institute of Mathematics and Natural Sciences, Tallinn University, Narva Rd 25, 10120, Tallinn, Estonia.
authors.forename: N
authors.initials: N
authors.lastname: Kadõrova
authors.affiliation: Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51014, Tartu, Estonia.
authors.forename: U
authors.initials: U
authors.lastname: Saarma
mesh_terms: D000818:Animals; D001208:Asia; D001947:Breeding; D004272:DNA, Mitochondrial / Q000235:genetics; D004957:Estonia; D005060:Europe; D005387:Finland; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D008297:Male; D058974:Phylogeography; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*; D034561:Sheep, Domestic / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial
keywords: Iron Age; Middle Ages; Ovis aries; Y-chromosome; ancient DNA; mitochondrial DNA; sheep domestication