title: The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs.
abstract: This study aimed to investigate if autologous bone marrow mesenchymal stem cells (MSCs) could treat osteonecrosis of the femoral head (ONFH) and what the fate and distribution of the cells are in dogs. Twelve Beagle dogs were randomly divided into two groups: MSCs group and SHAM operated group. After three weeks, dogs in MSCs group and SHAM operated group were intra-arterially injected with autologous MSCs and 0.9% normal saline, respectively. Eight weeks after treatment, the necrotic volume of the femoral heads was significantly reduced in MSCs group. Moreover, the trabecular bone volume was increased and the empty lacunae rate was decreased in MSCs group. In addition, the BrdU-positive MSCs were unevenly distributed in femoral heads and various vital organs. But no obvious abnormalities were observed. Furthermore, most of BrdU-positive MSCs in necrotic region expressed osteocalcin in MSCs group and a few expressed peroxisome proliferator-activated receptor-γ (PPAR-γ). Taken together, these data indicated that intra-arterially infused MSCs could migrate into the necrotic field of femoral heads and differentiate into osteoblasts, thus improving the necrosis of femoral heads. It suggests that intra-arterial infusion of autologous MSCs might be a feasible and relatively safe method for the treatment of femoral head necrosis.
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Hongting
authors.initials: H
authors.lastname: Jin
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Wu
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Pinger
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Shanxing
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Peijian
authors.initials: P
authors.lastname: Tong