title: The Effects of Co-transplantation of Olfactory Ensheathing Cells and Schwann Cells on Local Inflammation Environment in the Contused Spinal Cord of Rats.
abstract: Inflammatory response following spinal cord injury (SCI) is important in regulation of the repair process. Olfactory ensheathing cells (OECs) and Schwann cells (SCs) are important donor cells for repairing SCI in different animal models. However, synergistic or complementary effects of co-transplantation of both cells for this purpose have not been extensively investigated. In the present study, we investigated the effects of co-transplantation of OECs and SCs on expression of pro- or anti-inflammatory factor and polarization of macrophages in the injured spinal cord of rats. Mixed cell suspensions containing OECs and SCs were transplanted into the injured site at 7 days after contusion at the vertebral T10 level. Compared with the DMEM, SC, or OEC group, the co-transplantation group had a more extensive distribution of the grafted cells and significantly reduced number of astrocytes, microglia/macrophage infiltration, and expression of chemokines (CCL2 and CCL3) at the injured site. The co-transplantation group also significantly increased arginase+/CD206+macrophages (IL-4) and decreased iNOS+/CD16/32+macrophages (IFN-γ), which was followed by higher IL-10 and IL-13 and lower IL-6 and TNF-α in their expression levels, a smaller cystic cavity area, and improved motor functions. These results indicate that OEC and SC co-transplantation could promote the shift of the macrophage phenotype from M(IFN-γ) to M(IL-4), reduce inflammatory cell infiltration in the injured site, and regulate inflammatory factors and chemokine expression, which provide a better immune environment for SCI repair.
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Jieyuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Kuijun
authors.initials: K
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Daping, Chongqing, 400042, China. bcli1118@yahoo.com.
authors.forename: Bingcang
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D060833:Cellular Microenvironment / Q000502:physiology*; D005260:Female; D007249:Inflammation / Q000473:pathology / Q000628:therapy; D009416:Nerve Regeneration / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D020127:Recovery of Function / Q000502:physiology; D012583:Schwann Cells / Q000502:physiology* / Q000637:transplantation*; D013119:Spinal Cord Injuries / Q000473:pathology / Q000628:therapy*; D016896:Treatment Outcome
keywords: Inflammatory response; Macrophage phenotypes; Olfactory ensheathing cells; Schwann cells; Spinal cord injury