title: Comparative studies on mitochondrial electron transport chain complexes of Sitophilus zeamais treated with allyl isothiocyanate and calcium phosphide.
abstract: With Sitophilus zeamais as the target organism, the present study for the first time attempted to elucidate the comparative effects between allyl isothiocyanate (AITC) and calcium phosphide (Ca3P2), exposure on mitochondrial electron transport chain (ETC.) complex I & IV and their downstream effects on enzymes relevant to reactive oxygen species (ROS). In vivo, both AITC and Ca3P2 inhibited complex I and IV with similar downstream effects. In contrast with Ca3P2, the inhibition of complex I caused by AITC was dependent on time and dose. In vitro, AITC inhibited complex IV more significantly than complex I. These results indicate that mitochondrial complex IV is the primary target of AITC, and that complex I is another potential target.
authors.affiliation: Research and Development Center of Biorational Pesticide, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Research and Development Center of Biorational Pesticide, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Research and Development Center of Biorational Pesticide, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Research and Development Center of Biorational Pesticide, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, PR China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Research and Development Center of Biorational Pesticide, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, PR China. Electronic address: zhxing1952@gmail.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D042967:Electron Transport Complex I / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D017879:Isothiocyanates / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D010720:Phosphines / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D048889:Weevils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D017879:Isothiocyanates; D010720:Phosphines; D017382:Reactive Oxygen Species; C004471:allyl isothiocyanate; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I
keywords: Allyl isothiocyanate; Fumigation; Mitochondrion electron transport chain; Phosphine; Sitophilus zeamais