title: Telomere attrition and restoration in the normal teleost Oryzias latipes are linked to growth rate and telomerase activity at each life stage.
abstract: Telomere shortening occurs when cells divide, both in vitro and in vivo. On the other hand, telomerase is able to maintain telomere length in cells by adding TTAGGG repeats to the ends of telomeres. However, the interrelationships existing among telomere length, telomerase activity and growth in vertebrates remain to be clarified. In the present study we measured telomere length (terminal restriction fragment length), telomerase activity and body growth of Oryzias latipes from the embryo stage until senescence. During the rapid growth stage (age 0-7 months), telomeres shortened in parallel with decreasing telomerase activity. Then, during adolescence (age 7 months - 1 year), telomeres lengthened quickly as growth slowed and telomerase activity increased. In the adult stage (age 1-4 years) characterized by little growth, telomerase activity decreased gradually and telomeres shortened. Our data indicate that telomere attrition and restoration are linked to growth and telomerase activity, and suggest that critical loss of telomere homeostasis is associated with mortality in this animal.
authors.affiliation: Department of Comprehensive Education in Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.|Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hatakeyama
authors.affiliation: Department of Comprehensive Education in Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamazaki
authors.affiliation: Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Izumiyama-Shimomura
authors.affiliation: Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Aida
authors.affiliation: Department of Basic Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
authors.forename: Hiroetsu
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Comprehensive Education in Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Tsuchida
authors.affiliation: Bioinformatics Group, Genome Center, and Division of Cancer Genomics, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Matsuura
authors.affiliation: Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Kaiyo
authors.initials: K
authors.lastname: Takubo
authors.affiliation: Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Ishikawa
mesh_terms: D000367:Age Factors; D000818:Animals; D029941:Fish Proteins / Q000378:metabolism*; D007700:Kinetics; D008018:Life Cycle Stages; D009990:Oryzias / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D059505:Telomere Homeostasis; D059506:Telomere Shortening
chemical_list: D029941:Fish Proteins; D019098:Telomerase
keywords: adolescence; aging; growth; medaka; telomerase; telomere