title: Kinetics and binding geometries of the complex between β2-microglobulin and its antibody: An AFM and SPR study.
abstract: β2-Microglobulin (B2M) is a human protein involved in the regulation of immune response and represents a useful biomarker for several diseases. Recently, anti-B2M monoclonal antibodies have been introduced as innovative therapeutic agents. A deeper understanding of the molecular interaction between the two partners could be of utmost relevance for both designing array-based analytical devices and improving current immunotherapies. A visualization at the nanoscale performed by Atomic Force Microscopy revealed that binding of B2M to the antibody occurred according to two preferred interaction geometries. Additionally, Atomic Force Spectroscopy and Surface Plasmon Resonance provided us with detailed information on the binding kinetics and the energy landscape of the complex, both at the single molecule level and in bulk conditions. Combination of these complementary techniques contributed to highlight subtle differences in the kinetics behaviour characterizing the complexes. Collectively, the results may deserve significant interest for designing, development and optimization of novel generations of nanobiosensor platforms.
authors.affiliation: Biophysics and Nanoscience Centre, Dipartimento DEB, Università della Tuscia, Viterbo, Italy.
authors.forename: Emilia
authors.initials: E
authors.lastname: Coppari
authors.affiliation: Biophysics and Nanoscience Centre, Dipartimento DEB, Università della Tuscia, Viterbo, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Santini
authors.affiliation: Biophysics and Nanoscience Centre, Dipartimento DEB, Università della Tuscia, Viterbo, Italy. Electronic address: bizzarri@unitus.it.
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Bizzarri
authors.affiliation: Biophysics and Nanoscience Centre, Dipartimento DEB, Università della Tuscia, Viterbo, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Cannistraro
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody / Q000276:immunology; D006801:Humans; D007700:Kinetics; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D001613:beta 2-Microglobulin
keywords: AFM; Antigen–antibody biorecognition; Atomic Force Spectroscopy; Surface Plasmon Resonance; β(2)-Microglobulin