title: Endogenous neurotrophin-3 promotes neuronal sprouting from dorsal root ganglia.
abstract: In the present study, we investigated the role of endogenous neurotrophin-3 in nerve terminal sprouting 2 months after spinal cord dorsal root rhizotomy. The left L1-5 and L7-S2 dorsal root ganglia in adult cats were exposed and removed, preserving the L6 dorsal root ganglia. Neurotrophin-3 was mainly expressed in large neurons in the dorsal root ganglia and in some neurons in spinal lamina II. Two months after rhizotomy, the number of neurotrophin-3-positive neurons in the spared dorsal root ganglia and the density of neurite sprouts emerging from these ganglia were increased. Intraperitoneal injection of an antibody against neurotrophin-3 decreased the density of neurite sprouts. These findings suggest that endogenous neurotrophin-3 is involved in spinal cord plasticity and regeneration, and that it promotes axonal sprouting from the dorsal root ganglia after spinal cord dorsal root rhizotomy.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China; Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6 People's Hospital, Shanghai, China; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Xu-Yang
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6 People's Hospital, Shanghai, China.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6 People's Hospital, Shanghai, China.
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6 People's Hospital, Shanghai, China.
authors.forename: Heng-Li
authors.identifier: 0000-0002-1219-4952
authors.initials: HL
authors.lastname: Tian
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Xiang-He
authors.initials: XH
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Zheng
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Qi-Chuan
authors.initials: QC
authors.lastname: Zhuge
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Wei-Xing
authors.identifier: 0000-0001-7463-4493
authors.initials: WX
authors.lastname: Hu
keywords: cats; dorsal root ganglion; nerve regeneration; nerve terminal; neural regeneration; neurotrophin-3; sensory neurons