title: Hydrolysed inulin alleviates the azoxymethane-induced preneoplastic aberrant crypt foci by altering selected intestinal microbiota in Sprague-Dawley rats.
abstract: Context Inulin, a non-digestible carbohydrate isolated from Helianthus tuberosus L. (Asteraceae), has been shown to alter the gut beneficial bacteria including Lactobacillus spp. and Bifidobacteria. Inulin also influences the activities of intestinal microbiota that could prevent the colon cancer development. Objective This study determines the effect of hydrolysed inulin with different degrees of polymerisation on alteration of intestinal microbiota and their activities on azoxymethane (AOM)-induced preneoplastic aberrant crypt foci (ACF) in rats. Materials and methods Seventy-two male Sprague-Dawley rats were randomly divided into six groups (three control and three AOM-treated groups) and the animal were fed with either a normal diet or diet containing 10% of long-chain inulin (InuL) or short-chain inulin (InuS), respectively, for 17 weeks. Colon cancer was induced in rats by injecting AOM subcutaneously at the 8th and 9th week of the study period. At the end of the experiment, cecal contents of rats were examined for selected microbiota, organic acids, putrefactive compounds and microbial enzymes. ACF formation was microscopically examined. Results The inulin diets significantly increased the weight and decreased the pH of the caecal content. The rats fed with InuL-supplemented diet showed approximately 2.9- and 6.8-fold increases in the biomass of Lactobacillus spp. and Bifidobacteria, respectively. Naive and AOM-treated rats fed with inulin-supplemented diet showed ∼1.3- and ∼2.2-fold decreases in the biomass of Escherichia coli and Salmonella enterica serovar Typhi, respectively. Inulins significantly decreased the colonic concentration of phenol, p-cresol and indole. Reduction in the activity of microbial enzymes such as β-glucuronidase, azoreductase and nitroreductase were observed in inulin-treated animals. Reduction in the ACF formation has been observed in inulin-treated groups. Discussion and conclusion The present study demonstrates that dietary administration of inulin reduces the formation of preneoplastic lesions in the colon, possibly by altering the microecology and microbial activities on carcinogenesis.
authors.affiliation: a Health Product Research and Development Unit , Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand ;
authors.forename: Thanawat
authors.initials: T
authors.lastname: Pattananandecha
authors.affiliation: a Health Product Research and Development Unit , Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand ;
authors.forename: Sasithorn
authors.initials: S
authors.lastname: Sirilun
authors.affiliation: a Health Product Research and Development Unit , Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand ;
authors.forename: Yodsawee
authors.initials: Y
authors.lastname: Duangjitcharoen
authors.affiliation: a Health Product Research and Development Unit , Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand ;
authors.forename: Bhagavathi Sundaram
authors.initials: BS
authors.lastname: Sivamaruthi
authors.affiliation: b Department of Pathobiology , Faculty of Science, Mahidol University , Bangkok , Thailand ;
authors.forename: Prasit
authors.initials: P
authors.lastname: Suwannalert
authors.affiliation: c Health Innovation Institute , Chiang Mai , Thailand.
authors.forename: Sartjin
authors.initials: S
authors.lastname: Peerajan
authors.affiliation: a Health Product Research and Development Unit , Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand ;
authors.forename: Chaiyavat
authors.initials: C
authors.lastname: Chaiyasut
mesh_terms: D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001397:Azoxymethane; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D058491:Bacterial Load; D018533:Biomass; D003106:Colon / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D006868:Hydrolysis; D007444:Inulin / Q000494:pharmacology*; D008297:Male; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D016588:Anticarcinogenic Agents; D007444:Inulin; D001397:Azoxymethane
keywords: Helianthus tuberosus L; prebiotics; probiotics