title: Polyunsaturated lysophosphatidic acid as a potential asthma biomarker.
abstract: Lysophosphatidic acid (LPA), a lipid mediator in biological fluids and tissues, is generated mainly by autotaxin that hydrolyzes lysophosphatidylcholine to LPA and choline. Total LPA levels are increased in bronchoalveolar lavage fluid from asthmatic lung, and are strongly induced following subsegmental bronchoprovocation with allergen in subjects with allergic asthma. Polyunsaturated molecular species of LPA (C22:5 and C22:6) are selectively synthesized in the airways of asthma subjects following allergen challenge and in mouse models of allergic airway inflammation, having been identified and quantified by LC/MS/MS lipidomics. This review discusses current knowledge of LPA production in asthmatic lung and the potential utility of polyunsaturated LPA molecular species as novel biomarkers in bronchoalveolar lavage fluid and exhaled breath condensate of asthma subjects.
authors.affiliation: Department of Biochemistry & Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA.|Department of Medicine, University of Illinois at Chicago College of Medicine, Chicago, IL 60612, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Ackerman
authors.affiliation: Department of Medicine, University of Illinois at Chicago College of Medicine, Chicago, IL 60612, USA.
authors.forename: Gye Young
authors.initials: GY
authors.lastname: Park
authors.affiliation: Department of Medicine, Ohio State University School of Medicine, Columbus, OH 43210, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Christman
authors.affiliation: Department of Medicine, University of Illinois at Chicago College of Medicine, Chicago, IL 60612, USA.
authors.forename: Sharmilee
authors.initials: S
authors.lastname: Nyenhuis
authors.affiliation: Department of Medicine, National Jewish Health, Denver, CO 80206, USA.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Berdyshev
authors.affiliation: Department of Medicine, University of Illinois at Chicago College of Medicine, Chicago, IL 60612, USA.|Department of Pharmacology, University of Illinois at Chicago College of Medicine, Chicago, IL 60612, USA.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Natarajan
mesh_terms: D000328:Adult; D056151:Airway Remodeling; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000175:diagnosis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000503:physiopathology; D008168:Lung / Q000378:metabolism* / Q000503:physiopathology; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008246:Lysophospholipids / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D054466:Phospholipases A1 / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000378:metabolism; D000099189:Lysophospholipase D
chemical_list: D000485:Allergens; D015415:Biomarkers; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D054466:Phospholipases A1; D010727:Phosphoric Diester Hydrolases; D049368:Receptors, Lysophosphatidic Acid; D000099189:Lysophospholipase D; C032881:lysophosphatidic acid
keywords: LPA receptors; airway epithelium; allergic airway inflammation; asthma biomarkers; autotaxin; bronchoalveolar lavage fluid; eosinophils; exhaled breath condensate; lysophosphatidic acid (LPA); macrophages