title: AHSA1 regulates proliferation, apoptosis, migration, and invasion of osteosarcoma.
abstract: Activator of 90kDa heat shock protein ATPase homolog 1 (AHSA1) is a chaperone of heat shock 90kDa (HSP90) and stimulates ATPase activity of HSP90. The function of AHSA1 in osteosarcoma (OS) has not been reported yet. A previous study showed AHSA1 was overexpressed in OS cells. In this study, we investigated the role of AHSA1 in OS cells by silencing AHSA1. We report that silencing AHSA1 inhibited cell growth, migration, and invasion, and increased apoptosis of MG-63 and Saos2 cells. We also found that silencing AHSA1 decreased the ATPase activity of HSP90 in OS cells. In addition, silencing AHSA1 increased the levels of negative regulators of Wnt/β-catenin signalling pathway, Axin-2 and GSK3β, and decreased the levels of two key members of Wnt/β-catenin signalling pathway, namely, Wnt-5a and β-catenin. In conclusion, silencing AHSA1 regulates cell growth, apoptosis, migration, and invasion by regulating Wnt/β-catenin signalling pathway and their negative regulators.
authors.affiliation: Department of Orthopedic and Traumatology, First Affiliated Hospital, Jinan University, Guangzhou 510630, PR China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Shao
authors.affiliation: Department of Oncology, First Affiliated Hospital, Jinan University, Guangzhou 510630, PR China; Key laboratory of State Administration of Traditional Chinese Medicine, Department of Pathophysiology, Jinan University, Guangzhou 510630, PR China. Electronic address: wangliangmd@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou 510632, PR China; Department of Traumatology and Plastic Surgery, The Affiliated Jiangmen Traditional Chinese Medicine Hospital of Jinan University, Jiangmen 529000, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Key laboratory of State Administration of Traditional Chinese Medicine, Department of Pathophysiology, Jinan University, Guangzhou 510630, PR China.
authors.forename: Renbin
authors.initials: R
authors.lastname: Qi
authors.affiliation: Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou 510632, PR China; Department of Traumatology and Plastic Surgery, The Affiliated Jiangmen Traditional Chinese Medicine Hospital of Jinan University, Jiangmen 529000, PR China. Electronic address: wyzyykjk@126.com.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Li
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis; D017209:Apoptosis; D048708:Cell Growth Processes; D045744:Cell Line, Tumor; D002465:Cell Movement; D020868:Gene Silencing; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D060449:Wnt Signaling Pathway / Q000502:physiology
chemical_list: C515652:AHSA1 protein, human; D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D034741:RNA, Small Interfering; D000251:Adenosine Triphosphatases
keywords: AHSA1; Cell growth; Invasion; Migration; Osteosarcoma