title: Characterisation and in vitro antimicrobial potential of liposome encapsulated silver ions against Candida albicans.
abstract: Liposomes are biocompatible, biodegradable, controlled delivery systems with the ability to encapsulate both lipophilic and hydrophilic compounds, including metal ions. Liposome encapsulated Ag(+) (lipo-Ag(+)), prepared by reverse-phase evaporation, was used as a controlled delivery system against Candida albicans. Characterisation of the lipo-Ag(+) indicated that the multilamellar vesicles with diameters ranging between ≈ 0.5 and 5.0 μm showed potential as a controlled delivery system to consistently deliver Ag(+) to C. albicans. Results from inductively coupled plasma (ICP) analysis showed higher association of cell bound Ag(+) at 15 mins post exposure when compared to unencapsulated Ag(+). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicate detrimental effects of Ag(+) on C. albicans cell structure. These effects along with the ICP results also correlate with previously reported time kill experiment observations.
authors.affiliation: a School of Pharmacy, University of Wolverhampton , Wolverhampton , UK ;
authors.forename: W L
authors.initials: WL
authors.lastname: Low
authors.affiliation: a School of Pharmacy, University of Wolverhampton , Wolverhampton , UK ;|b Research Institute in Healthcare Science, University of Wolverhampton , Wolverhampton , UK ;
authors.forename: M A
authors.initials: MA
authors.lastname: Kenward
authors.affiliation: c Department of Biology, Chemistry and Forensic Sciences, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hill
authors.affiliation: a School of Pharmacy, University of Wolverhampton , Wolverhampton , UK ;|b Research Institute in Healthcare Science, University of Wolverhampton , Wolverhampton , UK ;
authors.forename: C
authors.initials: C
authors.lastname: Martin
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002177:Candidiasis / Q000188:drug therapy; D002414:Cations, Monovalent / Q000008:administration & dosage / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D012834:Silver / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D002414:Cations, Monovalent; D003692:Delayed-Action Preparations; D008081:Liposomes; D012834:Silver
keywords: Candida albicans; controlled release; liposomes; silver; wound management