title: Simple and rapid quantification of phospholipids for supramolecular membrane transport assays.
abstract: We introduce a simple (1)H NMR method for quantification of the phospholipid content of liposomes. The method is validated by comparison with the established Stewart assay, which revealed significant uncertainties in phospholipid quantification of established liposome preparations used in supramolecular membrane transport assays.
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany. a.hennig@jacobs-university.de.
authors.forename: Robert
authors.initials: R
authors.lastname: Hein
authors.forename: Can B
authors.initials: CB
authors.lastname: Uzundal
authors.forename: Andreas
authors.initials: A
authors.lastname: Hennig
mesh_terms: D001681:Biological Assay / Q000379:methods*; D001692:Biological Transport; D057230:Limit of Detection; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000737:chemistry*; D013997:Time Factors
chemical_list: D008081:Liposomes; D010743:Phospholipids