title: Ericoside, a new antibacterial biflavonoid from Erica mannii (Ericaceae).
abstract: A new dihydroflavonol-flavonol biflavonoid derivative, named ericoside was isolated from the ethanol extract of the whole plant of Erica mannii along with the known flavonoid, taxifolin 3-O-α-L-rhamnopyranoside; and two readily available sterols (sitosterol, sitosterol 3-O-β-D-glucopyranoside). The isolation was performed using chromatographic methods and the structure of purified molecules were elucidated using spectroscopic techniques (e.g. MS, NMR) and by comparison with literature data. The crude ethanol extract, ericoside, and taxifolin 3-O-α-L-rhamnopyranoside were tested against ten Gram-negative bacteria including multidrug resistant clinical isolates using a broth microdilution method. The crude ethanol extract showed no noteworthy activity. Of the purified compounds, ericoside displayed moderate activity against the resistant Escherichia coli AG100 with a MIC of 64 μg/mL.
authors.affiliation: Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: Gabin Thierry M
authors.initials: GT
authors.lastname: Bitchagno
authors.affiliation: Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: Simplice B
authors.initials: SB
authors.lastname: Tankeo
authors.affiliation: Food Science and Nutrition, Department of Chemistry, Carleton University, Ottawa, ON, Canada.
authors.forename: Apollinaire
authors.initials: A
authors.lastname: Tsopmo
authors.affiliation: Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: James D
authors.initials: JD
authors.lastname: Simo Mpetga
authors.affiliation: Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O. Box 6163, Yaounde, Cameroon.
authors.forename: Alembert T
authors.initials: AT
authors.lastname: Tchinda
authors.affiliation: Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Serge Alain T
authors.initials: SA
authors.lastname: Fobofou
authors.affiliation: Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: Antoine Honoré L
authors.initials: AH
authors.lastname: Nkuete
authors.affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Ludger A
authors.initials: LA
authors.lastname: Wessjohann
authors.affiliation: Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: Victor
authors.initials: V
authors.lastname: Kuete
authors.affiliation: Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon. Electronic address: ptane@yahoo.com.
authors.forename: Pierre
authors.initials: P
authors.lastname: Tane
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D044946:Biflavonoids / Q000737:chemistry* / Q000302:isolation & purification; D002163:Cameroon; D029791:Ericaceae / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D044946:Biflavonoids; D010936:Plant Extracts; C000608989:ericoside
keywords: Antibacterial activity; Biflavonoid; Erica mannii; Ericaceae; Ericoside