title: Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos.
abstract: Manipulation and culture of early mouse embryos is a powerful yet largely under-utilized technology enhancing the value of this model system. Conversely, cell culture has been widely used in developmental biology studies. However, it is important to determine whether in vitro cultured cells truly represent in vivo cell types. Grafting cells into embryos, followed by an assessment of their contribution during development is a useful method to determine the potential of in vitro cultured cells. In this study, we describe a method for grafting cells into a defined site of early postimplantation mouse embryos, followed by ex vivo culture. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing precise localization and adjustment of the number of cells receiving exogenous DNA with both high transfection efficiency and low cell death. These techniques, which do not require any specialized equipment, render experimental manipulations of the gastrulation and early organogenesis-stage mouse embryo possible, allowing analysis of commitment in cultured cell subpopulations and the effect of genetic manipulations in situ on cell differentiation.
authors.affiliation: MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh; yali.huang@ed.ac.uk.
authors.forename: Yali
authors.initials: Y
authors.lastname: Huang
authors.affiliation: MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh.
authors.forename: Ron
authors.initials: R
authors.lastname: Wilkie
authors.affiliation: MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh.
authors.forename: Valerie
authors.initials: V
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D017690:Cell Transplantation / Q000379:methods*; D004247:DNA / Q000008:administration & dosage*; D018274:Electroporation / Q000379:methods*; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005260:Female; D051379:Mice; D038081:Organogenesis / Q000235:genetics / Q000502:physiology; D011247:Pregnancy; D014162:Transfection
chemical_list: D004247:DNA