title: Determination of ribavirin in chicken muscle by quick, easy, cheap, effective, rugged and safe method and liquid chromatography-tandem mass spectrometry.
abstract: A new analytical method for the determination of ribavirin in chicken muscle using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method and liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed and validated. Samples were extracted with acidified methanol (methanol:acetic acid, 99:1, v/v). The extract was further purified by QuEChERS method using primary-secondary amine (PSA) and C18. Finally, the extract was dried by nitrogen under 45°C and reconstituted in water. The separation was performed on a Hypercarb analytical column under a gradient elution. The mobile phase was composed of water buffered with ammonium acetate (2.0mM) and acetonitrile. The proposed method was validated according to the European Commission Decision 2002/657/EC. The values of the decision limit (CCα) and the detection capability (CCβ) were 1.1 and 1.5μg/kg, respectively. The mean recoveries of ribavirin ranged from 94.2% to 99.2%. The repeatability (expressed as coefficient of variation, CVr) of the method ranged from 4.5% to 4.9% and the reproducibility (CVR) of the method ranged from 4.8% to 5.4%. The method is demonstrated to be suitable for the determination of ribavirin in chicken muscle in conformity with the current EU performance requirements through validation. The total time required for the analysis of one sample, including sample preparation, was about 45min.
authors.affiliation: Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China. Electronic address: wupaddyfield@tom.com.
authors.forename: Yin-Liang
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China.
authors.forename: Ruo-Xia
authors.initials: RX
authors.lastname: Chen
authors.affiliation: Yinzhou Agricultural Information and Agricultural Products Quality Inspection Service Center, Ningbo 315100, PR China.
authors.forename: Lie
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D015203:Reproducibility of Results; D012254:Ribavirin / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
chemical_list: D012254:Ribavirin
keywords: Chicken muscle; LC–MS/MS; QuEChERS; Ribavirin