title: Lamin-Binding Proteins in Caenorhabditis elegans.
abstract: The nuclear lamina, composed of lamins and numerous lamin-associated proteins, is required for mechanical stability, mechanosensing, chromatin organization, developmental gene regulation, mRNA transcription, DNA replication, nuclear assembly, and nuclear positioning. Mutations in lamins or lamin-binding proteins cause at least 18 distinct human diseases that affect specific tissues such as muscle, adipose, bone, nerve, or skin, and range from muscular dystrophies to lipodystrophy, peripheral neuropathy, or accelerated aging. Caenorhabditis elegans has unique advantages in studying lamin-binding proteins. These advantages include the low complexity of genes encoding lamin and lamin-binding proteins, advanced transgenic techniques, simple application of RNA interference, sophisticated genetic strategies, and a large collection of mutant lines. This chapter provides detailed and comprehensive protocols for the genetic and phenotypic analysis of lamin-binding proteins in C. elegans.
authors.affiliation: Andalusian Center for Developmental Biology, CSIC-Junta de Andalucia-Universidad Pablo de Olavide, Carretera de Utrera, Seville, Spain.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Dobrzynska
authors.affiliation: Andalusian Center for Developmental Biology, CSIC-Junta de Andalucia-Universidad Pablo de Olavide, Carretera de Utrera, Seville, Spain. Electronic address: pask@upo.es.
authors.forename: Peter
authors.initials: P
authors.lastname: Askjaer
authors.affiliation: Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. Electronic address: gru@vms.huji.ac.il.
authors.forename: Yosef
authors.initials: Y
authors.lastname: Gruenbaum
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D005818:Genetic Engineering; D034882:Lamins / Q000235:genetics; D034881:Nuclear Lamina / Q000502:physiology; D028861:Nuclear Pore Complex Proteins / Q000502:physiology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D034882:Lamins; D028861:Nuclear Pore Complex Proteins
keywords: BAF-1; CRISPR; ChIP; DamID; Emerin; FLIP; FRET; Lamin