title: Antioxidant Activity of Grapevine Leaf Extracts against Oxidative Stress Induced by Carbon Tetrachloride in Cerebral Cortex, Hippocampus and Cerebellum of Rats.
abstract: In recent years, it has become increasingly important to study the beneficial properties of derivatives of grapes and grapevine. The objective of this study was to determine the antioxidant activity of Vitis labrusca leaf extracts, comparing conventional and organic grapevines, in different brain areas of rats. We used male Wistar rats treated with grapevine leaf extracts for a period of 14 days, and on the 15th day, we administered in half of the rats, mineral oil and the other half, carbon tetrachloride (CCl₄). The animals were euthanized by decapitation and the cerebral cortex, hippocampus and cerebellum were removed to assess oxidative stress parameters and the activity of antioxidant enzymes. Lipid peroxidation levels (TBARS) were unchanged. However, CCl₄ induced oxidative damage to proteins in all tissues studied, and this injury was prevented by both extracts. Superoxide dismutase (SOD) activity was increased by CCl₄ in the cerebral cortex and decreased in other tissues. However, CCl₄ increased catalase (CAT) activity in the cerebellum and decreased it in the cerebral cortex. The SOD/CAT ratio was restored in the cerebellum by both extracts and only in the cerebral cortex by the organic extract.
authors.affiliation: Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil. marianefw@yahoo.com.br.
authors.forename: Mariane
authors.initials: M
authors.lastname: Wohlenberg
authors.affiliation: Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil. danielacamposdealmeida@gmail.com.
authors.forename: Daniela
authors.initials: D
authors.lastname: Almeida
authors.affiliation: Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil. lianevvb@gmail.com.
authors.forename: Liane
authors.initials: L
authors.lastname: Bokowski
authors.affiliation: Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil. niarasm@yahoo.com.br.
authors.forename: Niara
authors.initials: N
authors.lastname: Medeiros
authors.affiliation: Universidade de Caxias do Sul, Francisco Getúlio Vargas 1130, Caxias do Sul 95070-560, Brazil. mestrefabi@yahoo.com.br.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Agostini
authors.affiliation: Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil. csfunchal@yahoo.com.br.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Funchal
authors.affiliation: Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil. caroline.dani@metodistadosul.edu.br.
authors.forename: Caroline
authors.initials: C
authors.lastname: Dani
keywords: Vitis labrusca; grapes; neuroprotection; total polyphenols