title: Cardioprotective Effects of Astragalin against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart.
abstract: This study aims to evaluate the cardioprotective effects of astragalin against myocardial ischemia/reperfusion (I/R) injury in isolated rat heart. The cardioprotective effects of astragalin on myocardial I/R injury were investigated on Langendorff apparatus. Adult male Sprague-Dawley rats were randomly divided into five groups. The results showed that astragalin pretreatment improved myocardial function. Compared with I/R group, lactate dehydrogenase (LDH) and creatine kinase (CK) activities in coronary flow decreased in astragalin pretreatment groups, whereas superoxide dismutase (SOD) activity and glutathione/glutathione disulfide (GSH/GSSG) ratio significantly increased. The levels of malondialdehyde (MDA), intracellular reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) decreased in astragalin-treated groups. The infarct size (IS) and apoptosis rate in hearts from astragalin-treated groups were lower than those in hearts from the I/R group. Western blot analysis also revealed that astragalin preconditioning significantly reduced Bax level, whereas Bcl-2 was increased in the myocardium. Therefore, astragalin exhibited cardioprotective effects via its antioxidative, antiapoptotic, and anti-inflammatory activities.
authors.affiliation: Department of Cardiac Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China.
authors.forename: Daoxu
authors.initials: D
authors.lastname: Qu
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, Pharmacy School, Shihezi University, Shihezi 832002, China.
authors.forename: Jichun
authors.identifier: 0000-0002-2650-0519
authors.initials: J
authors.lastname: Han
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, Pharmacy School, Shihezi University, Shihezi 832002, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Ren
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Cardiac Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, Pharmacy School, Shihezi University, Shihezi 832002, China.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Department of Cardiac Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003326:Coronary Circulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D006334:Heart Function Tests; D006352:Heart Ventricles / Q000187:drug effects / Q000503:physiopathology; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology; D044949:Kaempferols / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009203:Myocardial Infarction / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D044949:Kaempferols; D051028:bcl-2-Associated X Protein; D008315:Malondialdehyde; C001579:astragalin; D013482:Superoxide Dismutase; D005978:Glutathione