title: [Detection of respiratory viruses in children with acute lower respiratory tract infection: an analysis of 5,150 children].
abstract: OBJECTIVE
  To investigate the distribution of respiratory viruses on throat swabs in hospitalized children with acute lower respiratory tract infection (ALRTI).
  
  
  METHODS
  A total of 5,150 children with ALRTI who were admitted to Hebei Children's Hospital between March 2014 and February 2015 were enrolled to investigate the distribution of respiratory viruses in children with ALRTI. Direct immunofluorescence assay was performed for throat swabs from these children to detect influenza virus A (FA), influenza virus B (FB), adenovirus (ADV), respiratory syncytial virus (RSV), and parainfluenza virus types 1, 2, and 3 (PIV-1, PIV-2, and PIV-3).
  
  
  RESULTS
  Of all the 5,150 throat swabs from hospitalized children, 2,155 (41.84%) had positive virus detection results. RSV had the highest detection rate (1,338 cases/25.98%), followed by PIV-3 (439 cases/8.52%) and FA (166 cases/3.22%), and 29 patients had mixed infection with 2 viruses. With the increasing age, the detection rates of viruses tended to decrease (χ2=279.623; P<0.01). The positive rate of RSV increased gradually from September, and reached the peak value (60.09%) in November; the lowest positive rate occurred in June (1.51%). The positive rate of PIV-3 was the highest in May (21.38%) and the lowest in November (1.77%).
  
  
  CONCLUSIONS
  The distribution of viruses in children with ALRTI varies with age and season, with RSV prevalence in autumn and winter and PIV-3 prevalence in spring and summer. RSV is the most common viral pathogen that causes ALRTI in hospitalized children.
authors.affiliation: Department of Pulmonology, Hebei Children's Hospital, Shijiazhuang 050031. mxyz2000@163.com.
authors.forename: Quan-Heng
authors.initials: QH
authors.lastname: Li
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Gao
authors.forename: Jin-Ying
authors.initials: JY
authors.lastname: Li
authors.forename: Ling-Ai
authors.initials: LA
authors.lastname: Shi
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Hao
authors.forename: Sheng-Wang
authors.initials: SW
authors.lastname: Ge
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: An
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009975:Orthomyxoviridae / Q000302:isolation & purification; D010224:Parainfluenza Virus 3, Human / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000821:virology*; D012621:Seasons