title: ELASPIC web-server: proteome-wide structure-based prediction of mutation effects on protein stability and binding affinity.
abstract: ELASPIC is a novel ensemble machine-learning approach that predicts the effects of mutations on protein folding and protein-protein interactions. Here, we present the ELASPIC webserver, which makes the ELASPIC pipeline available through a fast and intuitive interface. The webserver can be used to evaluate the effect of mutations on any protein in the Uniprot database, and allows all predicted results, including modeled wild-type and mutated structures, to be managed and viewed online and downloaded if needed. It is backed by a database which contains improved structural domain definitions, and a list of curated domain-domain interactions for all known proteins, as well as homology models of domains and domain-domain interactions for the human proteome. Homology models for proteins of other organisms are calculated on the fly, and mutations are evaluated within minutes once the homology model is available.
  
  
  
  The ELASPIC webserver is available online at http://elaspic.kimlab.org
  
  
  
  pm.kim@utoronto.ca or pi@kimlab.orgSupplementary data: Supplementary data are available at Bioinformatics online.
authors.affiliation: Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Department of Molecular Genetics, University of Toronto.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Witvliet
authors.affiliation: Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Department of Computer Science, University of Toronto, Toronto, ON, Canada and.
authors.forename: Alexey
authors.initials: A
authors.lastname: Strokach
authors.affiliation: Department of Electrical and Electronic Engineering, Universidad Nacional De Colombia, Manizales, Colombia.
authors.forename: Andrés Felipe
authors.initials: AF
authors.lastname: Giraldo-Forero
authors.affiliation: Donnelly Center for Cellular and Biomolecular Research, University of Toronto.
authors.forename: Joan
authors.initials: J
authors.lastname: Teyra
authors.affiliation: Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Department of Computer Science, University of Toronto, Toronto, ON, Canada and.
authors.forename: Recep
authors.initials: R
authors.lastname: Colak
authors.affiliation: Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Department of Molecular Genetics, University of Toronto, Department of Computer Science, University of Toronto, Toronto, ON, Canada and.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Kim
mesh_terms: D006801:Humans; D009154:Mutation; D011485:Protein Binding; D017510:Protein Folding; D055550:Protein Stability; D020543:Proteome; D012984:Software
chemical_list: D020543:Proteome