title: The Fdb3 transcription factor of the Fusarium Detoxification of Benzoxazolinone gene cluster is required for MBOA but not BOA degradation in Fusarium pseudograminearum.
abstract: A number of cereals produce the benzoxazolinone class of phytoalexins. Fusarium species pathogenic towards these hosts can typically degrade these compounds via an aminophenol intermediate, and the ability to do so is encoded by a group of genes found in the Fusarium Detoxification of Benzoxazolinone (FDB) cluster. A zinc finger transcription factor encoded by one of the FDB cluster genes (FDB3) has been proposed to regulate the expression of other genes in the cluster and hence is potentially involved in benzoxazolinone degradation. Herein we show that Fdb3 is essential for the ability of Fusarium pseudograminearum to efficiently detoxify the predominant wheat benzoxazolinone, 6-methoxy-benzoxazolin-2-one (MBOA), but not benzoxazoline-2-one (BOA). Furthermore, additional genes thought to be part of the FDB gene cluster, based upon transcriptional response to benzoxazolinones, are regulated by Fdb3. However, deletion mutants for these latter genes remain capable of benzoxazolinone degradation, suggesting that they are not essential for this process.
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia; University of Queensland (UQ), School of Agriculture and Food Sciences, St. Lucia, Queensland 4067, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Kettle
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Carere
authors.affiliation: University of Queensland (UQ), School of Agriculture and Food Sciences, St. Lucia, Queensland 4067, Australia; University of Western Australia, School of Plant Biology, Crawley, Western Australia 6009, Australia.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Batley
authors.affiliation: CSIRO Agriculture, Black Mountain, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: John M
authors.initials: JM
authors.lastname: Manners
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia; UQ, Queensland Alliance for Agriculture & Food Innovation, St. Lucia, Brisbane, Queensland 4067, Australia.
authors.forename: Kemal
authors.initials: K
authors.lastname: Kazan
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia. Electronic address: Donald.Gardiner@csiro.au.
authors.forename: Donald M
authors.initials: DM
authors.lastname: Gardiner
mesh_terms: D001583:Benzoxazoles / Q000378:metabolism* / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008658:Inactivation, Metabolic; D005810:Multigene Family; D010935:Plant Diseases; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism / Q000382:microbiology*
chemical_list: D001583:Benzoxazoles; D014157:Transcription Factors; C017057:benzoxazolone; C031896:6-methoxybenzoxazolinone
keywords: Fusarium graminearum; Fusarium verticillioides; Phytoalexin; Phytoanticipin