title: Chlorogenic acid ameliorates intestinal mitochondrial injury by increasing antioxidant effects and activity of respiratory complexes.
abstract: Dietary polyphenols are thought to be beneficial for human health by acting as antioxidants. Chlorogenic acid (CGA) is abundant in plant-based foods as an ester of caffeic acid and quinic acid. In this study, we investigated the effects of CGA on mitochondrial protection. Our results demonstrated that pretreatment with CGA ameliorated the intestinal mitochondrial injury induced by H2O2; membrane potential was increased, mitochondrial swelling, levels of reactive oxygen species, contents of 8-hydroxy-2-deoxyguanosine, and cytochrome c released were decreased. The beneficial effects of CGA were accompanied by an increase in antioxidant and respiratory-chain complex I, IV, and V activities. In trinitrobenzene-sulfonic acid-induced colitic rats indicated that CGA supplementation improved mitochondria ultrastructure and decreased mitochondrial injury. Our results suggest a promising role for CGA as a mitochondria-targeted antioxidant in combating intestinal oxidative injury. Daily intake of diets containing CGA, such as coffee and honeysuckle, may be useful for prevention of intestinal diseases.
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Mi
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Li
authors.affiliation: c Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.|b College of Food Science and Technology , Nanchang University , Nanchang , China.|c Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha , China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045304:Cytochromes c / Q000378:metabolism*; D042967:Electron Transport Complex I / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004789:Enzyme Activation; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008933:Mitochondrial Swelling / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D014302:Trinitrobenzenesulfonic Acid; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D005978:Glutathione
keywords: chlorogenic acid; intestinal injury; mitochondria; respiratory chain complex