title: Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease.
abstract: Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer (P<0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 μg pDNA + 64 μg D-SPM and 40 μg pDNA at 4 weeks postvaccination (P>0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia; Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Firouzamandi
authors.affiliation: Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Hassan
authors.initials: H
authors.lastname: Moeini
authors.affiliation: Razi Vaccine and Serum Research Institute, Arak, Iran.
authors.forename: Seyed Davood
authors.initials: SD
authors.lastname: Hosseini
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Mohd Hair
authors.initials: MH
authors.lastname: Bejo
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia; Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.affiliation: Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Mehrbod
authors.affiliation: Biomedical Research Center, Vice President Office for Research, Qatar University, Doha, Qatar.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: El Zowalaty
authors.affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia; Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Aini
authors.initials: A
authors.lastname: Ideris
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D003911:Dextrans / Q000737:chemistry*; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009521:Newcastle Disease / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology; D010063:Ovum / Q000276:immunology*; D013096:Spermine / Q000737:chemistry*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D000914:Antibodies, Viral; D003911:Dextrans; D006388:Hemagglutinins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D013096:Spermine
keywords: DNA vaccine; Newcastle disease; Newcastle disease virus; dextran-spermine nanoparticle; hemagglutinin and fusion; in ovo vaccination
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