title: An immunoassay for urinary extracellular vesicles.
abstract: Although nanosized urinary extracellular vesicles (uEVs) are increasingly used for biomarker discovery, their isolation currently relies on time-consuming techniques hindering high-throughput application. To navigate this problem, we designed an immunoassay to isolate, quantify, and normalize uEV proteins. The uEV immunoassay consists of a biotinylated CD9 antibody to isolate uEVs, an antibody against the protein of interest, and two conjugated antibodies to quantify the protein of interest and CD9. As a proof of principle, the immunoassay was developed to analyze the water channel aquaporin-2 (AQP2) and the sodium-chloride cotransporter (NCC). CD9 was used as a capture antibody because immunoprecipitation showed that anti-CD9 antibody, but not anti-CD63 antibody, isolated AQP2 and NCC. CD9 correlated strongly with urine creatinine, allowing CD9 to be used for normalization of spot urines. The uEV immunoassay detected AQP2 and NCC with high sensitivity, low coefficients of variance, and stability in dilution series. After water loading in healthy subjects, the uEV immunoassay detected decreases in AQP2 and NCC equally well as the traditional method using ultracentrifugation and immunoblot. The uEV immunoassay also reliably detected lower and higher AQP2 or NCC levels in uEVs from patients with pathological water or salt reabsorption, respectively. In summary, we report a novel approach to analyze uEVs that circumvents existing isolation and normalization issues, requires small volumes of urine, and detects anticipated changes in physiological responses and clinical disorders.
authors.affiliation: Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Salih
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus, Denmark.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Fenton
authors.affiliation: Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Knipscheer
authors.affiliation: Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Joost W
authors.initials: JW
authors.lastname: Janssen
authors.affiliation: Department of Urology, Erasmus Medical Center, Rotterdam, The Netherlands; and.
authors.forename: Mirella S
authors.initials: MS
authors.lastname: Vredenbregt-van den Berg
authors.affiliation: Department of Urology, Erasmus Medical Center, Rotterdam, The Netherlands; and.
authors.forename: Guido
authors.initials: G
authors.lastname: Jenster
authors.affiliation: Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Robert
authors.initials: R
authors.lastname: Zietse
authors.affiliation: Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands; e.j.hoorn@erasmusmc.nl.
authors.forename: Ewout J
authors.initials: EJ
authors.lastname: Hoorn
mesh_terms: D055354:Exosomes / Q000378:metabolism*; D000067128:Extracellular Vesicles / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods*
keywords: CD9; aquaporin-2; biomarker; exosomes; microvesicles; sodium-chloride cotransporter