title: Photonic Crystal Optical Tweezers with High Efficiency for Live Biological Samples and Viability Characterization.
abstract: We propose and demonstrate a new optical trapping method for single cells that utilizes modulated light fields to trap a wide array of cell types, including mammalian, yeast, and Escherichia coli cells, on the surface of a two-dimensional photonic crystal. This method is capable of reducing the required light intensity, and thus minimizing the photothermal damage to living cells, thereby extending cell viability in optical trapping and cell manipulation applications. To this end, a thorough characterization of cell viability in optical trapping environments was performed. This study also demonstrates the technique using spatial light modulation in patterned manipulation of live cell arrays over a broad area.
authors.affiliation: Department of Electrical Engineering, University of Washington, Seattle WA, 98195, USA.
authors.forename: Peifeng
authors.initials: P
authors.lastname: Jing
authors.affiliation: Department of Electrical Engineering, University of Washington, Seattle WA, 98195, USA.
authors.forename: Jingda
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Bioengineering, University of Washington, Seattle WA, 98195, USA.
authors.forename: Gary W
authors.initials: GW
authors.lastname: Liu
authors.affiliation: Department of Electrical Engineering, University of Washington, Seattle WA, 98195, USA.
authors.forename: Ethan G
authors.initials: EG
authors.lastname: Keeler
authors.affiliation: Department of Bioengineering, University of Washington, Seattle WA, 98195, USA.
authors.forename: Suzie H
authors.initials: SH
authors.lastname: Pun
authors.affiliation: Department of Electrical Engineering, University of Washington, Seattle WA, 98195, USA.
authors.forename: Lih Y
authors.initials: LY
authors.lastname: Lin
mesh_terms: D000818:Animals; D001419:Bacteria; D002470:Cell Survival; D051379:Mice; D041681:NIH 3T3 Cells; D052898:Optical Tweezers; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*; D017785:Photons; D015003:Yeasts
references.citation: Nature. 2003 Aug 14;424(6950):810-6
references.pmid: 12917694
references.citation: Nat Cell Biol. 2001 Apr;3(4):339-45
references.pmid: 11283606
references.citation: Lab Chip. 2014 Feb 21;14(4):646-52
references.pmid: 24322181
references.citation: Nano Lett. 2009 Oct;9(10):3387-91
references.pmid: 19159322
references.citation: J Cell Biol. 1975 Jul;66(1):188-93
references.pmid: 49354
references.citation: Nature. 2007 Apr 26;446(7139):1066-9
references.pmid: 17460669
references.citation: Rev Sci Instrum. 2004 Sep;75(9):2787-809
references.pmid: 16878180
references.citation: Nature. 2005 Jul 21;436(7049):370-2
references.pmid: 16034413
references.citation: Nat Protoc. 2006;1(2):755-68
references.pmid: 17406305
references.citation: Nano Lett. 2013 May 8;13(5):2290-4
references.pmid: 23581875
references.citation: Lab Chip. 2008 Jul;8(7):1034-41
references.pmid: 18584076
references.citation: Lab Chip. 2011 Feb 21;11(4):645-51
references.pmid: 21180703
references.citation: J Bacteriol. 2000 Oct;182(19):5551-5
references.pmid: 10986260
references.citation: Nature. 2003 Jan 23;421(6921):423-7
references.pmid: 12540915
references.citation: Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20691-6
references.pmid: 21068372
references.citation: Lab Chip. 2011 Dec 21;11(24):4174-80
references.pmid: 22038401
references.citation: Opt Express. 2004 Dec 13;12(25):6208-14
references.pmid: 19488265
references.citation: Nano Lett. 2012 Mar 14;12(3):1633-7
references.pmid: 22283484
references.citation: Opt Express. 2010 Jul 19;18(15):16005-13
references.pmid: 20720985
references.citation: Biophys J. 1999 Nov;77(5):2856-63
references.pmid: 10545383
references.citation: Phys Rev E Stat Nonlin Soft Matter Phys. 2008 Aug;78(2 Pt 1):021910
references.pmid: 18850868
references.citation: Opt Express. 2009 Apr 13;17(8):6018-24
references.pmid: 19365421
references.citation: Nano Lett. 2010 Jan;10(1):99-104
references.pmid: 19957918