title: Genome-wide analysis of differential mRNA expression of Amsacta moorei entomopoxvirus, mediated by the gene encoding a viral protein kinase (AMV197).
abstract: Insect-born entomopoxviruses (Fam: Poxviridae) are potentially important bio-pesticide against insect pests and expression vectors as well as vectors for transient human gene therapies including recombinant viral vaccines. For these reasons, it is necessary to understand the regulatory genes functions to improve its biotechnological potential. Here, we focused on the characterization of serine/threonine (Ser/Thr; ORF AMV197) protein kinase gene from the Amsacta moorei entomopoxvirus (AMEV), the type species of the genus Betaentomopoxvirus. Transcription of the parental and an amv197-null recombinant AMEV was compared by whole-genome gene expression microarray analysis. Blast2GO analysis reflected a broad diversity of upregulated and downregulated genes. Results showed that expression levels of 102 genes (45%) out of 226 tested genes changed significantly in the recombinant AMEV infected cells. Of these transcripts, 72 (70.58%) were upregulated and 30 (29.41%) were downregulated throughout the infection period. Genes involved in DNA repair, replication and nucleotide metabolism, transcription and RNA modification, and protein modification were mostly upregulated at different times in cells infected with the recombinant virus. Furthermore, transcription of all studied cellular genes including metabolism of apoptosis (Nedd2-like caspase, hemolin and elongation factor-1 alpha (ef1a) gene) was downregulated in the absence of amv197. Quantitative real time reverse transcription-PCR confirmed viral transcriptional changes obtained by microarray. The results of this study indicated that the product of amv197 appears to affect the transcriptional regulation of most viral and many cellular genes. Further investigations are, however, needed to narrow down the role of AMV197 throughout the infection process.
authors.affiliation: Karadeniz Technical University, Faculty of Sciences, Department of Molecular Biology and Genetic, 61080 Trabzon, Turkey.
authors.forename: Hacer
authors.initials: H
authors.lastname: Muratoglu
authors.affiliation: Karadeniz Technical University, Faculty of Sciences, Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Remziye
authors.initials: R
authors.lastname: Nalcacioglu
authors.affiliation: Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste. Marie, Ontario, Canada.
authors.forename: Basil M
authors.initials: BM
authors.lastname: Arif
authors.affiliation: Karadeniz Technical University, Faculty of Sciences, Department of Biology, 61080 Trabzon, Turkey. Electronic address: zihni@ktu.edu.tr.
authors.forename: Zihni
authors.initials: Z
authors.lastname: Demirbag
mesh_terms: D017996:Entomopoxvirinae / Q000235:genetics*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D046228:Microarray Analysis; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D012333:RNA, Messenger; D011494:Protein Kinases
keywords: Amsacta moorei entomopoxvirus (AMEV); AmΔPK197/gfp; Gene expression; Insect virus; Protein kinase