title: [Expression of heat shock protein 70 and its mRNA in career exposure to manganese].
abstract: OBJECTIVE
  To analyze the expression levels of heat shock protein70 (HSPs70) and HSPs70 mRNA in different exposure to manganese, and research the neuroprotective effect on the career exposure to manganese.
  
  
  METHODS
  From 2008 to 2009, with cross-sectional study design, and in a locomotive and rolling stock works, by stratified random sampling method, the exposed sample consisted of 180 welders from different welding shops and 100 unexposed in the last three years, non-welder controls with age-matched workers of similar socioeconomic status from the same industry. The control workers had not been exposed to neurotoxic chemicals. The mRNA expressions of four different metabolic enzyme were detected by SYBR Green I quantitative real-time polymerase chain reaction. The expression levels of the two enzymes mRNA in different exposure to manganese were analyzed. The expressions of HSPs70 were detected by Western blot. The concentration of air manganese was determined by GFAAS. The average concentration of 8 h time (8h-TWA) was used to express the level of individual exposure to manganese, according to the air manganese workplace occupational exposure limit (8h-TWA=0.15 mg/m3), the exposed group is divided into high exposed group (>0.15 mg/m3) and low exposure group (<0.15 mg/m3).
  
  
  RESULTS
  The individuals exposed to manganese dose of exposed group ((0.25±0.31) mg/m3) was higher than the control group ((0.06±0.02) mg/m3) (t=6.15, P=0.001); individuals exposed to manganese dose of high exposure group for (0.42±0.34) mg/m3, which was higher than low exposure group (0.09±0.07) mg/m3 (t=9.80, P=0.001). HSPs70 mRNA and protein of exposure group (5.65±0.21, 3.26±0.15) were higher than the reference group (0.41±0.03, 1.32±0.12) (t=18.91, t=8.68, P=0.001). HSP70 mRNA and protein of high exposure group (6.48±0.37, 3.67±0.26) were higher than the low exposure group (5.15±0.23, 3.02±0.19) (t=3.24, t=2.01, P=0.003, P=0.043).
  
  
  CONCLUSIONS
  The expression of peripheral blood lymphocytes HSPs70 level and HSPs70 mRNA workers exposed to manganese increased and protect nerve cells from related to Mn stimulation induced lipid peroxidation damag.
authors.affiliation: Occupation Health Inspection Evaluation Center, Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.affiliation: Email: chinashaohua5888@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Shao
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Chi
authors.forename: Zhihu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yongle
authors.initials: Y
authors.lastname: Shan
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
mesh_terms: D003430:Cross-Sectional Studies; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D008345:Manganese; D016273:Occupational Exposure; D012333:RNA, Messenger; D014896:Welding
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D008345:Manganese