title: [Histocompatibility of nano-hydroxyapatite/poly-co-glycolic acid tissue engineering bone modified by mesenchymal stem cells with vascular endothelial frowth factor].
abstract: OBJECTIVE
  To explorec Histocompatibility of nano-hydroxyapatite/poly-co-glycolic acid tissue engineering bone modified by mesenchymal stem cells with vascular endothelial frowth factor transinfected.
  
  
  METHODS
  Rat bone marrow mesenchymal stem cells (BMSCs) was separated, using BMSCs as target cells, and then vascular endothelial growth factor (VEGF) gene was transfected. Composite bone marrow mesenchymal stem cells and cells transfected with nano-hydroxyapatite (HA)/polylactic-co-glycolic acid (PLGA). The composition of cell and scaffold was observed.
  
  
  RESULTS
  The blank plasmid transfection was 39.1%, 40.1% in VEGF group. The cell adhesion and growth was found on the scaffold pore wall after 5 days, and the number of adherent cells in the nano-HA/PLGA composite scaffold material basically had no significant difference in both.
  
  
  CONCLUSIONS
  Although the nano-HA/PLGA scaffold material is still not fully meet the requirements of the matrix material for bone tissue engineering, but good biocompatibility, structure is its rich microporous satisfaction in material mechanics, toughening, enhanced obviously. Composition scaffold with BMSCs transfected by VEGF plasmid, the ability of angiogenesis is promoted.
authors.affiliation: Department of Orthopaedic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Minglei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China; Email: yrf_wind@jlu.edu.cn.
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yin
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002448:Cell Adhesion; D017886:Durapatite; D006016:Glycolates; D019344:Lactic Acid; D059630:Mesenchymal Stem Cells; D049329:Nanostructures; D011091:Polyesters; D011108:Polymers; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A
chemical_list: D006016:Glycolates; D011091:Polyesters; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; C031149:glycolic acid; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite