title: Microfluidic assay for precise measurements of mouse, rat, and human neutrophil chemotaxis in whole-blood droplets.
abstract: Animal models of human disease differ in innate immune responses to stress, pathogens, or injury. Precise neutrophil phenotype measurements could facilitate interspecies comparisons. However, such phenotype comparisons could not be performed accurately with the use of current assays, as they require the separation of neutrophils from blood using species-specific protocols, and they introduce distinct artifacts. Here, we report a microfluidic technology that enables robust characterization of neutrophil migratory phenotypes in a manner independent of the donor species and performed directly in a droplet of whole blood. The assay relies on the particular ability of neutrophils to deform actively during chemotaxis through microscale channels that block the advance of other blood cells. Neutrophil migration is measured directly in blood, in the presence of other blood cells and serum factors. Our measurements reveal important differences among migration counts, velocity, and directionality among neutrophils from 2 common mouse strains, rats, and humans.
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Caroline N
authors.initials: CN
authors.lastname: Jones
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Anh N
authors.initials: AN
authors.lastname: Hoang
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Martel
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Laurie
authors.initials: L
authors.lastname: Dimisko
authors.affiliation: Center for Comparative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Mikkola
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Naohide
authors.initials: N
authors.lastname: Kuriyama
authors.affiliation: Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Marina
authors.initials: M
authors.lastname: Yamada
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Bashar
authors.initials: B
authors.lastname: Hamza
authors.affiliation: Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: Masao
authors.initials: M
authors.lastname: Kaneki
authors.affiliation: Department of Pediatrics and Medicine, Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;
authors.forename: H Shaw
authors.initials: HS
authors.lastname: Warren
authors.affiliation: Center for Comparative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; and.
authors.forename: Diane E
authors.initials: DE
authors.lastname: Brown
authors.affiliation: BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; dirimia@hms.harvard.edu.
authors.forename: Daniel
authors.initials: D
authors.lastname: Irimia
mesh_terms: D000818:Animals; D054441:Cell Migration Assays, Leukocyte / Q000379:methods*; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D009504:Neutrophils / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
keywords: inbred lines; migration; neutrophil
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