title: Chemical constituents with anti-allergic activity from the root of Edulis Superba, a horticultural cultivar of Paeonia lactiflora.
abstract: The methanolic extract and its subfractions from the dried root of Edulis Superba, a horticultural cultivar of Paeonia lactiflora Pallas, showed potent anti-allergic effect as inhibition of immunoglobulin E (IgE)-mediated degranulation in rat basophil leukemia (RBL)-2H3 cells. Bioassay-guided fractionation led to the isolation of 26 compounds, including a new norneolignan glycoside, paeonibenzofuran (1), together with 25 known ones (2-26). The chemical structure of the new compound was elucidated on the basis of spectroscopic and chemical evidences. Among the isolated compounds, mudanpioside E (5) with paeoniflorin-type skeleton and quercetin (16) showed potent inhibitory activity against a degranulation marker, β-hexosaminidase release with IC50 values of 40.34 and 25.05 μM, respectively. A primary structure-activity relationship of these components was discussed.
authors.affiliation: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Shi
authors.affiliation: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan. szhu@inm.u-toyama.ac.jp.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Toyama Prefectural Medicinal Plants Center, Toyama, 930-0412, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Division of Gastrointestinal Pathophysiology, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Toyama, 930-0194, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kadowaki
authors.affiliation: The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, 305-0843, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshimatsu
authors.affiliation: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan. katsukok@inm.u-toyama.ac.jp.
authors.forename: Katsuko
authors.initials: K
authors.lastname: Komatsu
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001952:Bridged-Ring Compounds / Q000032:analysis; D045744:Cell Line, Tumor; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry* / Q000145:classification; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011794:Quercetin / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D013045:Species Specificity; D013329:Structure-Activity Relationship; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D015415:Biomarkers; D001952:Bridged-Ring Compounds; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; D011794:Quercetin; D001619:beta-N-Acetylhexosaminidases
keywords: Anti-allergic activity; Bioactive constituents; Horticultural cultivar; Paeonia lactiflora
references.citation: J Nat Med. 2015 Jan;69(1):142-7
references.pmid: 25300344
references.citation: Clin Exp Allergy. 2000 Apr;30(4):501-8
references.pmid: 10718847
references.citation: J Nat Med. 2012 Jan;66(1):39-48
references.pmid: 21647585
references.citation: Arch Pharm Res. 2014 Jun;37(6):698-705
references.pmid: 24155021
references.citation: Fitoterapia. 2014 Apr;94:29-35
references.pmid: 24462959
references.citation: Bioorg Med Chem. 2002 Oct;10(10):3123-8
references.pmid: 12150856
references.citation: Phytochemistry. 2008 May;69(8):1767-72
references.pmid: 18400238
references.citation: J Nat Prod. 1998 Mar;61(3):343-6
references.pmid: 9548873
references.citation: FEBS Lett. 2010 Jan 4;584(1):111-8
references.pmid: 19903473
references.citation: Fitoterapia. 2016 Jan;108:55-61
references.pmid: 26598138
references.citation: Chem Pharm Bull (Tokyo). 2007 Jun;55(6):899-901
references.pmid: 17541189
references.citation: Chem Biodivers. 2008 Sep;5(9):1723-35
references.pmid: 18816525
references.citation: Phytochemistry. 2000 Nov;55(6):597-601
references.pmid: 11130670
references.citation: J Nat Med. 2013 Jul;67(3):643-6
references.pmid: 22903244
references.citation: Arch Pharm Res. 2008 Apr;31(4):445-50
references.pmid: 18449501
references.citation: J Nat Med. 2015 Jan;69(1):35-45
references.pmid: 25151277
references.citation: J Nat Med. 2015 Apr;69(2):209-17
references.pmid: 25516374
references.citation: Arch Pharm Res. 2005 Jul;28(7):775-83
references.pmid: 16114491
references.citation: Bioorg Med Chem Lett. 2015 Nov 15;25(22):5087-91
references.pmid: 26483135
references.citation: J Nat Prod. 2003 Jun;66(6):759-63
references.pmid: 12828458
references.citation: Phytother Res. 2011 Sep;25(9):1301-5
references.pmid: 21312305
references.citation: Eur J Pharmacol. 2008 Feb 26;581(1-2):191-203
references.pmid: 18078925