title: Toxicity and inflammatory response in Swiss albino mice after intraperitoneal and oral administration of polyurethane nanoparticles.
abstract: In this work in vivo experiments were conducted in order to characterize the biocompatibility of polyurethane nanoparticles (PU-NPs) after intraperitoneal (i.p.) and oral administration. Additionally, ex vivo assays were performed to assess human blood compatibility as well as in vitro assays to assess protein binding. Our results indicated that administration of three different concentrations of PU-NPs induced a significant increase in visceral fat accumulation after oral dosing. In addition, fat tissue of mice intraperitoneally treated with the highest concentration of nanoparticles showed diffuse mononuclear inflammatory infiltrate in the fat tissue. Histopathological assessment showed inflammatory infiltrate and hepatocyte vacuolization in the liver, inflammatory infiltration and vascular congestion in the lung and glomerular necrosis in the kidney. Hepatic enzymes related with liver function were significantly increased in both groups of mice treated with PU-NPs. The PU-NPs did not affect the human blood cells number as well as coagulation time but showed a susceptibility to bind in proteins commonly found in the blood stream. In addition, increased amounts of pro inflammatory cytokines in vivo, as well as ex vivo in human cells were observed. Further studies to establish the consequences of long-term exposure to PU-NPs are warranted.
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina P.O. Box 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Adny H
authors.initials: AH
authors.lastname: Silva
authors.affiliation: Curso de Farmácia, Universidade do Oeste de Santa Catarina, Videira, SC, 89560-000, Brazil.
authors.forename: Claudriana
authors.initials: C
authors.lastname: Locatelli
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina P.O. Box 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Fabíola B
authors.initials: FB
authors.lastname: Filippin-Monteiro
authors.affiliation: Departament of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool, L693GF, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: Martin
authors.affiliation: Departament of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool, L693GF, UK.
authors.forename: Neill J
authors.initials: NJ
authors.lastname: Liptrott
authors.affiliation: Nanovetores Tecnologia S.A., SC 401, km1, ParqTecAlfa, Florianópolis, SC, 88030-000, Brazil.
authors.forename: Betina G
authors.initials: BG
authors.lastname: Zanetti-Ramos
authors.affiliation: Departamento de Física, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Luana C
authors.initials: LC
authors.lastname: Benetti
authors.affiliation: Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, SC, 88040-900, Brazil.
authors.forename: Evelize M
authors.initials: EM
authors.lastname: Nazari
authors.affiliation: Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, SC, 88040-900, Brazil.
authors.forename: Cláudia A C
authors.initials: CA
authors.lastname: Albuquerque
authors.affiliation: Departamento de Física, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: André A
authors.initials: AA
authors.lastname: Pasa
authors.affiliation: Departament of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool, L693GF, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Owen
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina P.O. Box 476, Florianópolis, SC, 88040-900, Brazil. Electronic address: tania.pasa@ufsc.br.
authors.forename: Tânia B
authors.initials: TB
authors.lastname: Creczynski-Pasa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000633:toxicity*; D011140:Polyurethanes / Q000008:administration & dosage / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D011140:Polyurethanes; D014409:Tumor Necrosis Factor-alpha
keywords: Biocompatibility; Inflammation; Nanoparticles; Toxicity; in vitro; in vivo