title: Utility of Western Blot Analysis for the Diagnosis of Cutaneous Leishmaniasis.
abstract: BACKGROUND
  Cutaneous leishmaniasis (CL) is a parasitic disease with a relatively wide distribution in different areas of the world, including Iran. The parasite is mainly diagnosed microscopically, but serological approaches might be useful for diagnosis as well. This study aimed to assess the efficacy of an immunoblotting system for serodiagnosis of cutaneous leishmaniasis in Iran.
  
  
  METHODS
  Sixty-one sera samples from parasitologically confirmed CL patients and 50 sera samples from healthy controls along with 50 sera sample from non-CL patients were collected. Native strain of Leishmania major was cultured in Schneider medium and soluble Leishmania antigens were prepared from amastigotes-like parasites. All of sera samples were evaluated by an immunoblotting system.
  
  
  RESULTS
  Components of 14 to 135 kDa were detectable by the sera of CL patients. From 61 sera of CL patients, 59 cases (96.7%) detected a 63 kDa subunit and 51 cases (83.6%) recognized a 32-35 kDa component. Among all subunits, the 63 kDa band showed the highest sensitivity (96.7%) and a 75 kDa band had the highest (98%) specificity.
  
  
  CONCLUSIONS
  Immunoblotting has a satisfactory performance in diagnosis of CL and this test can be used, as an aid, for proper diagnosis of CL.
authors.affiliation: Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Ashrafmansouri
authors.affiliation: Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Bahador
authors.initials: B
authors.lastname: Sarkari
authors.affiliation: Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Hatam
authors.affiliation: Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Habibi
authors.affiliation: Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Abdolahi Khabisi
keywords: Cutaneous Leishmaniasis; ELISA; IFA; Immunoblotting
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