title: Galectin expression in healing wounded skin treated with low-temperature plasma: Comparison with treatment by electronical coagulation.
abstract: Low-temperature plasma is useful for the care of wounded skin. It accelerates wound healing. However, the mechanism of this effect has not been fully elucidated yet. Galectin-1 is reported to accelerate wound healing via the Smad signaling pathway. In the present study to clarify whether or not galectins were expressed during the process of wound healing in the plasma-treated skin, we examined the effect of low-temperature plasma on galectin expression in the healing skin. We compared the effects of low-temperature plasma on the expression of galectin-1, -2, and -3 in the healing skin with those of electrocoagulation conducted with a high-frequency electrical coagulator. Immediately after the start of low-temperature plasma treatment following the incision made in the skin, a membrane-like structure was formed on the surface of the wound. Immunoelectron microscopy showed that these galectins were localized in the membrane-like structure of the plasma-treated skin. The expressions of these galectins were increased by the low-temperature plasma treatment, whereas they were inhibited by the electrocoagulation. These results suggest that galectins were involved in the wound healing of low-temperature plasma-treated skin. Galectins will thus be good markers for further examination of the effects of low-temperature plasma on the healing of wounded skin.
authors.affiliation: Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181-8611, Japan. Electronic address: yakimoto@ks.kyorin-u.ac.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Akimoto
authors.affiliation: Biotechnology Research Institute for Drug Discovery, The National Institutes of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Sanae
authors.initials: S
authors.lastname: Ikehara
authors.affiliation: Biotechnology Research Institute for Drug Discovery, The National Institutes of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Electronics and Photonics Research Institute, AIST, Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181-8611, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.affiliation: International University of Health and Welfare, Sanno Hospital, Akasaka, 107-0052, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Graduate School of Engineering, Nagoya University, Nagoya, 464-8603, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Hori
authors.affiliation: Electronics and Photonics Research Institute, AIST, Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Sakakita
authors.affiliation: Biotechnology Research Institute for Drug Discovery, The National Institutes of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan; Graduate School of Medicine, Chiba University, Chiba, 107-0052, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D003080:Cold Temperature; D004564:Electrocoagulation; D005260:Female; D037483:Galectin 1 / Q000378:metabolism*; D037501:Galectin 2 / Q000378:metabolism*; D037502:Galectin 3 / Q000378:metabolism*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D058626:Plasma Gases / Q000627:therapeutic use*; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism / Q000473:pathology; D051785:Smad Proteins / Q000378:metabolism; D014945:Wound Healing
chemical_list: D037483:Galectin 1; D037501:Galectin 2; D037502:Galectin 3; D058626:Plasma Gases; D051785:Smad Proteins
keywords: Electrocoagulation; Galectin; Immunohistochemistry; Low-temperature plasma; Skin; Wound healing