title: Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells.
abstract: Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Kin Weng
authors.initials: KW
authors.lastname: Kong
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Sarni
authors.initials: S
authors.lastname: Mat-Junit
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Norhaniza
authors.initials: N
authors.lastname: Aminudin
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia , Serdang, Selangor , Malaysia.
authors.forename: Fouad Abdulrahman
authors.initials: FA
authors.lastname: Hassan
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia , Serdang, Selangor , Malaysia.
authors.forename: Amin
authors.initials: A
authors.lastname: Ismail
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Azlina
authors.initials: A
authors.lastname: Abdul Aziz
keywords: Antioxidant enzymes; Barringtonia racemosa; HPLC-ESI-MS; Lipid peroxidation; Oxidative stress; Polyphenols
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