title: Giardia duodenalis genotypes in domestic and wild animals from Romania identified by PCR-RFLP targeting the gdh gene.
abstract: Sixty Giardia duodenalis isolates from domestic (n=49) and wild (n=11) animals (dogs, cats, deers, wolves, raccoon dog and muskrat) were analysed by PCR-RFLP at glutamate dehydrogenase locus (gdh). The isolates were obtained from positive feces samples for Giardia cysts analysed by flotation technique with saturated sodium chloride solution (specific gravity 1.28). Three G. duodenalis genotypes were identified: C (10/60; 16.7%); D (42/60; 70.0%); and E (7/60; 11.7%). In dogs all three genotypes were found, with the following prevalences: 76.9% genotype D (30/39); 23.1% C (9/39); 2.6% genotype E (1/39). One dog was co-infected with C and D genotypes. In cats we identified only G. duodenalis genotype D. Wolves and raccoon dog harbored infection with G. duodenalis genotype D, deers with E type and muskrat C type. This is the first study regarding genotyping of G. duodenalis in cats and wild animals from Romania. To the best of our knowledge, this is the first report of assemblages E in roe deers; assemblage C in wolves and muskrat; and assemblage D in raccoon dog.
authors.affiliation: Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania. Electronic address: titilincua@yahoo.com.
authors.forename: Gyӧrke
authors.initials: G
authors.lastname: Adriana
authors.affiliation: Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania.
authors.forename: Kalmár
authors.initials: K
authors.lastname: Zsuzsa
authors.affiliation: Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania. Electronic address: mirabela.dumitrache@usamvcluj.com.
authors.forename: Dumitrache
authors.initials: D
authors.lastname: Mirabela Oana
authors.affiliation: Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania.
authors.forename: Gherman Călin
authors.initials: GC
authors.lastname: Mircea
authors.affiliation: Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania.
authors.forename: Mircean
authors.initials: M
authors.lastname: Viorica
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D000835:Animals, Wild / Q000469:parasitology*; D005838:Genotype; D005872:Giardia / Q000235:genetics*; D005873:Giardiasis / Q000469:parasitology*; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D058507:Host Specificity; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012383:Romania
chemical_list: D005969:Glutamate Dehydrogenase
keywords: Carnivores; Deers; Genotypes; Giardia duodenalis; Muskrat; RFLP