title: Extinction and Retrieval + Extinction of Conditioned Fear Differentially Activate Medial Prefrontal Cortex and Amygdala in Rats.
abstract: Pairing a previously neutral conditioned stimulus (CS; e.g., a tone) to an aversive unconditioned stimulus (US; e.g., a footshock) leads to associative learning such that the tone alone comes to elicit a conditioned response (e.g., freezing). We have previously shown that an extinction session that occurs within the reconsolidation window (termed retrieval + extinction) attenuates fear responding and prevents the return of fear in Pavlovian fear conditioning (Monfils et al., 2009). To date, the mechanisms that explain the different behavioral outcomes between standard extinction and retrieval + extinction remain poorly understood. Here we sought to examine the differential temporal engagement of specific neural systems by these two approaches using Arc catFISH (cellular compartment analysis of temporal activity using fluorescence in situ hybridization (FISH)). Our results demonstrate that extinction and retrieval + extinction lead to differential patterns of expression, suggesting that they engage different networks. These findings provide insight into the neural mechanisms that allow extinction during reconsolidation to prevent the return of fear in rodents.
authors.affiliation: Department of Psychology, The University of Texas at Austin Austin, TX, USA.
authors.forename: Hongjoo J
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Psychological and Brain Sciences, The Johns Hopkins University Baltimore, MD, USA.
authors.forename: Rebecca P
authors.initials: RP
authors.lastname: Haberman
authors.affiliation: Department of Psychology, The University of Texas at Austin Austin, TX, USA.
authors.forename: Rheall F
authors.initials: RF
authors.lastname: Roquet
authors.affiliation: Department of Psychology, The University of Texas at AustinAustin, TX, USA; Center for Learning and Memory, The University of Texas at AustinAustin, TX, USA.
authors.forename: Marie-H
authors.initials: MH
authors.lastname: Monfils
keywords: Arc catFISH; extinction; fear conditioning; reconsolidation; retrieval + extinction
references.citation: Learn Mem. 2014 Oct 15;21(11):580-4
references.pmid: 25320349
references.citation: Science. 2000 Jan 14;287(5451):248-51
references.pmid: 10634773
references.citation: Neuron. 2006 Nov 9;52(3):437-44
references.pmid: 17088210
references.citation: Nature. 2002 Nov 7;420(6911):70-4
references.pmid: 12422216
references.citation: Behav Neurosci. 1992 Apr;106(2):274-85
references.pmid: 1590953
references.citation: Learn Mem. 2010 Sep 30;17(10):512-21
references.pmid: 20884753
references.citation: Front Behav Neurosci. 2013 Dec 09;7:186
references.pmid: 24367304
references.citation: Neurobiol Learn Mem. 2014 Nov;115:78-85
references.pmid: 25196703
references.citation: Nature. 2010 Jan 7;463(7277):49-53
references.pmid: 20010606
references.citation: Science. 2010 Nov 19;330(6007):1108-12
references.pmid: 21030604
references.citation: Neuropsychopharmacology. 2011 Jan;36(2):529-38
references.pmid: 20962768
references.citation: Science. 2012 Apr 13;336(6078):241-5
references.pmid: 22499948
references.citation: Neuron. 1997 Sep;19(3):613-24
references.pmid: 9331352
references.citation: J Neurosci. 2005 Sep 7;25(36):8295-302
references.pmid: 16148237
references.citation: J Neurosci. 2015 Feb 25;35(8):3607-15
references.pmid: 25716859
references.citation: Learn Mem. 2009 Jul 24;16(8):486-93
references.pmid: 19633138
references.citation: J Exp Psychol Anim Behav Process. 1975 Jan;1(1):88-96
references.pmid: 1151290
references.citation: J Exp Psychol Anim Behav Process. 1979 Oct;5(4):368-78
references.pmid: 528893
references.citation: Nat Neurosci. 2009 Mar;12(3):256-8
references.pmid: 19219038
references.citation: Nat Neurosci. 2011 Sep 11;14(10):1302-8
references.pmid: 21909089
references.citation: J Neurosci. 2000 Jun 1;20(11):3993-4001
references.pmid: 10818134
references.citation: J Neurosci. 2002 Dec 1;22(23 ):10067-71
references.pmid: 12451105
references.citation: Front Behav Neurosci. 2013 Dec 02;7:179
references.pmid: 24348354
references.citation: Nat Neurosci. 1999 Dec;2(12):1120-4
references.pmid: 10570490
references.citation: Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20040-5
references.pmid: 24277809
references.citation: Nature. 2000 Aug 17;406(6797):722-6
references.pmid: 10963596
references.citation: J Neurosci. 2007 Sep 26;27(39):10445-55
references.pmid: 17898216
references.citation: Science. 2009 May 15;324(5929):951-5
references.pmid: 19342552
references.citation: Psychopharmacology (Berl). 2013 Apr;226(4):631-47
references.pmid: 23404065
references.citation: Neurobiol Learn Mem. 2012 Mar;97(3):338-45
references.pmid: 22406658