title: The inactivation domain of STIM1 is functionally coupled with the Orai1 pore to enable Ca2+-dependent inactivation.
abstract: The inactivation domain of STIM1 (ID(STIM): amino acids 470-491) has been described as necessary for Ca(2+)-dependent inactivation (CDI) of Ca(2+) release-activated Ca(2+) (CRAC) channels, but its mechanism of action is unknown. Here we identify acidic residues within IDSTIM that control the extent of CDI and examine functional interactions of ID(STIM) with Orai1 pore residues W76 and Y80. Alanine scanning revealed three IDSTIM residues (D476/D478/D479) that are critical for generating full CDI. Disabling ID(STIM) by a triple alanine substitution for these three residues ("STIM1 3A") or by truncation of the entire domain (STIM1(1-469)) reduced CDI to the same residual level observed for the Orai1 pore mutant W76A (approximately one third of the extent seen with full-length STIM1). Results of noise analysis showed that STIM11-469 and Orai1 W76A mutants do not reduce channel open probability or unitary Ca(2+) conductance, factors that determine local Ca(2+) accumulation, suggesting that they diminish CDI instead by inhibiting the CDI gating mechanism. We tested for functional coupling between ID(STIM) and the Orai1 pore by double-mutant cycle analysis. The effects on CDI of mutations disabling ID(STIM) or W76 were not additive, demonstrating that ID(STIM) and W76 are strongly coupled and act in concert to generate full-strength CDI. Interestingly, disabling ID(STIM) and W76 separately gave opposite results in Orai1 Y80A channels: channels with W76 but lacking ID(STIM) generated approximately two thirds of the WT extent of CDI but those with ID(STIM) but lacking W76 completely failed to inactivate. Together, our results suggest that Y80 alone is sufficient to generate residual CDI, but acts as a barrier to full CDI. Although ID(STIM) is not required as a Ca(2+) sensor for CDI, it acts in concert with W76 to progress beyond the residual inactivated state and enable CRAC channels to reach the full extent of inactivation.
authors.affiliation: Department of Pathology and Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 rslewis@stanford.edu fmullins@stanford.edu.
authors.forename: Franklin M
authors.initials: FM
authors.lastname: Mullins
authors.affiliation: Department of Pathology and Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 rslewis@stanford.edu fmullins@stanford.edu.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Lewis
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002460:Cell Line; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009363:Neoplasm Proteins / Q000378:metabolism*; D000071740:ORAI1 Protein; D017434:Protein Structure, Tertiary; D000071737:Stromal Interaction Molecule 1
chemical_list: D015220:Calcium Channels; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D000071740:ORAI1 Protein; C510746:ORAI1 protein, human; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1; D002118:Calcium
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