title: Adsorption of plasma proteins and fibronectin on poly(hydroxylethyl methacrylate) brushes of different thickness and their relationship with adhesion and migration of vascular smooth muscle cells.
abstract: The surface-grafted poly(hydroxylethyl methacrylate) (PHEMA) molecules were demonstrated to show a brush state regardless of their molecular length (molecular weight). Adsorption of proteins from 10% fetal bovine serum (FBS), fibronectin (Fn) and bovine serum albumin (BSA) was quantified by ellipsometry, revealing that the amounts of FBS and Fn decreased monotonously along with the increase of PHEMA thickness, whereas not detectable for BSA when the PHEMA thickness was larger than 6 nm. Radio immunoassay found that the adsorption of Fn from 10% FBS had no significant difference regardless of the PHEMA thickness. However, ELISA results showed that the Arg-Gly-Asp (RGD) activity of adsorbed Fn decreased with the increase of PHEMA thickness. By comparison of cellular behaviors of vascular smooth muscle cells (VSMCs) being cultured in vitro in the normal serum-containing medium and the Fn-depleted serum-containing medium, the significant role of Fn on modulating the adhesion and migration of VSMCs was verified. Taking account all the results, the Fn adsorption model and its role on linking the biomaterials surface to the VSMCs behaviors are proposed.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Tanchen
authors.initials: T
authors.lastname: Ren
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Zhu
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Mao
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
keywords: migration; poly(hydroxylethyl methacrylate); protein adsorption; smooth muscle cells
references.citation: Langmuir. 2013 May 28;29(21):6386-95
references.pmid: 23634666
references.citation: Langmuir. 2011 Apr 19;27(8):4906-13
references.pmid: 21405141
references.citation: Biomaterials. 2001 Jan;22(2):99-111
references.pmid: 11101155
references.citation: Biomaterials. 2005 Jun;26(16):3227-34
references.pmid: 15603817
references.citation: Cell. 1988 May 20;53(4):649-57
references.pmid: 3286012
references.citation: J Biomed Mater Res. 1993 Aug;27(8):1103-13
references.pmid: 8408123
references.citation: Langmuir. 2004 Sep 14;20(19):8279-86
references.pmid: 15350103
references.citation: Biomaterials. 2012 Jan;33(3):810-20
references.pmid: 22048007
references.citation: J Cell Biol. 1965 Jul;26(1):137-55
references.pmid: 5859016
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2588-90
references.pmid: 10077553
references.citation: Biomaterials. 2013 Jan;34(4):975-84
references.pmid: 23127331
references.citation: Biomaterials. 2009 May;30(13):2457-67
references.pmid: 19181378
references.citation: Biophys J. 2000 Jul;79(1):144-52
references.pmid: 10866943
references.citation: Nature. 1984 Sep 20-26;311(5983):267-9
references.pmid: 6482952
references.citation: Colloids Surf B Biointerfaces. 2010 Aug 1;79(1):291-7
references.pmid: 20462745
references.citation: Ann N Y Acad Sci. 1998 Oct 23;857:119-29
references.pmid: 9917837
references.citation: Acta Biomater. 2009 Jul;5(6):1864-71
references.pmid: 19342321
references.citation: Langmuir. 2007 Oct 23;23(22):11168-73
references.pmid: 17892312
references.citation: Colloids Surf B Biointerfaces. 2008 Dec 1;67(2):210-5
references.pmid: 18838254
references.citation: Colloids Surf B Biointerfaces. 2014 Oct 1;122:134-42
references.pmid: 25033433
references.citation: Biomaterials. 2011 Dec;32(34):8968-78
references.pmid: 21872325
references.citation: Biomaterials. 2011 Jun;32(16):3958-68
references.pmid: 21377726
references.citation: J Biol Chem. 1983 Dec 10;258(23):14539-44
references.pmid: 6643500
references.citation: Physiol Rev. 2004 Jul;84(3):767-801
references.pmid: 15269336
references.citation: Biomaterials. 2011 Apr;32(11):2775-85
references.pmid: 21272933
references.citation: J Biomed Mater Res A. 2003 Feb 1;64(2):349-56
references.pmid: 12522822
references.citation: J Cell Biol. 1981 Dec;91(3 Pt 1):673-78
references.pmid: 7328116
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12542-7
references.pmid: 12237407
references.citation: Biomaterials. 2010 Nov;31(32):8465-74
references.pmid: 20701964
references.citation: J R Soc Interface. 2012 Dec 7;9(77):3455-68
references.pmid: 22896570