title: Proteomic analysis of mitral valve in Lewis rat with acute rheumatic heart disease.
abstract: Rheumatic heart disease (RHD) makes a heavy burden in human lives and economy. The proteomic analysis of acute rheumatic heart disease (ARHD) can provide precious data to study RHD at the early stages, but no one has looked into. So based on our early research we applied the method of continuous GAS stimulation on Lewis rats to duplicate the animal model of ARHD. And the mitral valves of rats in control group (n=10) and ARHD group (n=10) were selected for proteomic analysis of ARHD with the iTRAQ labeling based 2D LC-ESI-MS/MS quantitative technology. We identified 3931 proteins in valve tissue out of which we obtained 395 differentially expressed proteins containing 176 up-regulated proteins and 119 down-regulated proteins. Changes in levels of GAPDH (6.793 times higher than the control group) and CD9 (2.63 times higher than the control group) were confirmed by Western blot or immunohistochemistry. The differentially expressed proteins such as GAPDH, CD9, myosin, collagen and RAC1 may be potential biomarkers for ARHD. Moreover, the mitral valve protein profile shed light on further understanding and investigating ARHD.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical UniversityNanning 530021, Guangxi, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical UniversityNanning, Guangxi 530021, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Gui
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Huilei
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Danping
authors.initials: D
authors.lastname: Gong
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D008943:Mitral Valve / Q000378:metabolism* / Q000382:microbiology; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011917:Rats, Inbred Lew; D012214:Rheumatic Heart Disease / Q000378:metabolism* / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D013297:Streptococcus pyogenes / Q000472:pathogenicity; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D011506:Proteins
keywords: Acute rheumatic heart disease; ITRAQ quantitative proteomics; animal model; mitral valve protein profile; mitral valve tissue
references.citation: J Cell Physiol. 2005 Jan;202(1):191-204
references.pmid: 15389525
references.citation: Lancet. 2012 Mar 10;379(9819):953-64
references.pmid: 22405798
references.citation: PLoS One. 2014;9(12):e116289
references.pmid: 25551757
references.citation: J Cell Biochem. 2006 Jan 1;97(1):56-70
references.pmid: 16229015
references.citation: J Biol Chem. 2004 Feb 13;279(7):5984-92
references.pmid: 14617633
references.citation: Biomed Res Int. 2014;2014:151726
references.pmid: 24738046
references.citation: Lancet Infect Dis. 2005 Nov;5(11):685-94
references.pmid: 16253886
references.citation: Proc Inst Mech Eng H. 2008 Jan;222(1):129-43
references.pmid: 18335724
references.citation: J Cell Sci. 2001 May;114(Pt 9):1643-53
references.pmid: 11309196
references.citation: Clin Exp Immunol. 2000 Aug;121(2):270-4
references.pmid: 10931141
references.citation: Z Kardiol. 2001;90 Suppl 3:43-6
references.pmid: 11374032
references.citation: Heart. 2001 Jan;85(1):13-7
references.pmid: 11119453
references.citation: Circulation. 1974 Jan;49(1):7-12
references.pmid: 4271711
references.citation: Nat Rev Cardiol. 2014 Feb;11(2):123
references.pmid: 24419260
references.citation: Biochem Soc Trans. 2011 Apr;39(2):559-62
references.pmid: 21428939
references.citation: Infect Immun. 2001 Jun;69(6):4072-8
references.pmid: 11349078
references.citation: Tex Heart Inst J. 2007;34(3):352-9
references.pmid: 17948087
references.citation: Can J Cardiol. 2014 Sep;30(9):962-70
references.pmid: 24986049
references.citation: PLoS One. 2013;8(8):e72111
references.pmid: 24015209
references.citation: Clin Exp Pharmacol Physiol. 2012 Aug;39(8):674-9
references.pmid: 21895736
references.citation: Biochem Soc Trans. 2011 Apr;39(2):547-52
references.pmid: 21428937
references.citation: J Infect Dis. 2001 Feb 1;183(3):507-11
references.pmid: 11133385
references.citation: Clin Proteomics. 2014 Sep 24;11(1):35
references.pmid: 25379033
references.citation: Circ Res. 2000 Nov 24;87(11):1055-62
references.pmid: 11090552
references.citation: Indian Heart J. 2014 May-Jun;66(3):320-6
references.pmid: 24973838
references.citation: Int Immunol. 2008 Mar;20(3):445-52
references.pmid: 18245783
references.citation: Rheumatol Int. 2010 Nov;30(12):1621-5
references.pmid: 20012632
references.citation: Proteomics. 2015 Aug;15(15):2560-7
references.pmid: 26033914
references.citation: J Immunol. 2010 Aug 1;185(3):1968-75
references.pmid: 20610654
references.citation: Ann Thorac Surg. 2003 Feb;75(2):457-65; discussion 465-6
references.pmid: 12607654
references.citation: Autoimmunity. 2001;34(3):193-7
references.pmid: 11908777
references.citation: Circulation. 1951 Dec;4(6):836-43
references.pmid: 14879491
references.citation: J Autoimmun. 2008 Sep;31(2):136-41
references.pmid: 18541406
references.citation: Surg Today. 2016 Mar;46(3):285-96
references.pmid: 25893771
references.citation: Biochem Soc Trans. 2011 Apr;39(2):563-7
references.pmid: 21428940
references.citation: J Mol Cell Cardiol. 1997 Aug;29(8):2245-51
references.pmid: 9281455
references.citation: Nat Rev Cardiol. 2013 Mar;10(3):171-7
references.pmid: 23319102
references.citation: Arthritis Rheum. 2010 Apr;62(4):1026-35
references.pmid: 20131272
references.citation: J Immunol. 2006 Oct 15;177(8):5652-8
references.pmid: 17015754