title: Immediate manifestation of acoustic trauma in the auditory cortex is layer specific and cell type dependent.
abstract: Exposure to loud sounds damages the auditory periphery and induces maladaptive changes in central parts of the auditory system. Diminished peripheral afferentation and altered inhibition influence the processing of sounds in the auditory cortex. It is unclear, however, which types of inhibitory interneurons are affected by acoustic trauma. Here we used single-unit electrophysiological recording and two-photon calcium imaging in anesthetized mice to evaluate the effects of acute acoustic trauma (125 dB SPL, white noise, 5 min) on the response properties of neurons in the core auditory cortex. Electrophysiological measurements suggested the selective impact of acoustic trauma on inhibitory interneurons in the auditory cortex. To further investigate which interneuronal types were affected, we used two-photon calcium imaging to record the activity of neurons in cortical layers 2/3 and 4, specifically focusing on parvalbumin-positive (PV+) and somatostatin-positive (SST+) interneurons. Spontaneous and pure-tone-evoked firing rates of SST+ interneurons increased in layer 4 immediately after acoustic trauma and remained almost unchanged in layer 2/3. Furthermore, PV+ interneurons with high best frequencies increased their evoked-to-spontaneous firing rate ratios only in layer 2/3 and did not change in layer 4. Finally, acoustic trauma unmasked low-frequency excitatory inputs only in layer 2/3. Our results demonstrate layer-specific changes in the activity of auditory cortical inhibitory interneurons within minutes after acoustic trauma.
authors.affiliation: Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ondrej.novak@biomed.cas.cz.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Novák
authors.affiliation: Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Zelenka
authors.affiliation: Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hromádka
authors.affiliation: Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Syka
mesh_terms: D000200:Action Potentials; D000818:Animals; D001303:Auditory Cortex / Q000166:cytology / Q000503:physiopathology*; D005072:Evoked Potentials, Auditory; D006317:Hearing Loss, Noise-Induced / Q000503:physiopathology*; D007395:Interneurons / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010320:Parvalbumins / Q000235:genetics / Q000378:metabolism; D013004:Somatostatin / Q000235:genetics / Q000378:metabolism
chemical_list: D010320:Parvalbumins; D013004:Somatostatin
keywords: broadband noise; calcium imaging; inhibition; interneurons; plasticity
references.citation: Neuron. 2001 Oct 11;32(1):141-9
references.pmid: 11604145
references.citation: Hear Res. 1998 Oct;124(1-2):78-84
references.pmid: 9822904
references.citation: J Neurophysiol. 2003 Oct;90(4):2387-401
references.pmid: 12773493
references.citation: Eur J Neurosci. 2015 Mar;41(5):665-76
references.pmid: 25728183
references.citation: Nat Neurosci. 2001 Jan;4(1):52-62
references.pmid: 11135645
references.citation: J Neurosci. 2014 Oct 8;34(41):13670-83
references.pmid: 25297094
references.citation: Hear Res. 2008 Nov;245(1-2):82-91
references.pmid: 18812219
references.citation: Hear Res. 1994 Aug;78(2):158-68
references.pmid: 7982808
references.citation: Nat Neurosci. 2000 Sep;3(9):904-10
references.pmid: 10966621
references.citation: Science. 1990 Apr 6;248(4951):73-6
references.pmid: 2321027
references.citation: PLoS One. 2013;8(3):e57247
references.pmid: 23516401
references.citation: J Neurosci. 2010 Nov 10;30(45):14972-9
references.pmid: 21068300
references.citation: Nature. 2012 Aug 16;488(7411):379-83
references.pmid: 22878719
references.citation: Hear Res. 1982 Dec;8(3):273-83
references.pmid: 7153182
references.citation: J Neurosci. 2012 Nov 14;32(46):16141-8
references.pmid: 23152598
references.citation: Hear Res. 1997 Feb;104(1-2):191-203
references.pmid: 9119763
references.citation: J Neurosci. 2013 Dec 11;33(50):19567-78
references.pmid: 24336721
references.citation: Nat Protoc. 2009;4(8):1128-44
references.pmid: 19617885
references.citation: Lab Anim. 2006 Apr;40(2):200-9
references.pmid: 16600080
references.citation: Eur J Neurosci. 2009 May;29(9):1921-30
references.pmid: 19473244
references.citation: Neuroscience. 2005;131(1):1-11
references.pmid: 15680687
references.citation: Exp Brain Res. 2001 Sep;140(2):145-61
references.pmid: 11521147
references.citation: Neuron. 2006 Jun 15;50(6):823-39
references.pmid: 16772166
references.citation: Opt Lett. 2009 Feb 1;34(3):362-4
references.pmid: 19183659
references.citation: Nat Neurosci. 2007 Apr;10(4):462-8
references.pmid: 17334362
references.citation: Front Neural Circuits. 2014;8:15
references.pmid: 24653677
references.citation: Neuron. 2013 Nov 20;80(4):1066-76
references.pmid: 24267655
references.citation: Hear Res. 2004 Jul;193(1-2):39-50
references.pmid: 15219319
references.citation: PLoS One. 2010;5(3):e9521
references.pmid: 20209092
references.citation: J Neurosci. 2009 Nov 4;29(44):13797-808
references.pmid: 19889991
references.citation: Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14974-9
references.pmid: 21896771
references.citation: Neuron. 2012 Jan 12;73(1):159-70
references.pmid: 22243754
references.citation: Trends Neurosci. 2004 Nov;27(11):676-82
references.pmid: 15474168
references.citation: Hear Res. 2000 Jan;139(1-2):59-68
references.pmid: 10601713
references.citation: Nat Neurosci. 2010 Mar;13(3):361-8
references.pmid: 20118924
references.citation: J Neurophysiol. 2008 Mar;99(3):1137-51
references.pmid: 18160422
references.citation: Science. 2014 Aug 1;345(6196):1255263
references.pmid: 25082707
references.citation: Dev Neurobiol. 2011 Jan 1;71(1):45-61
references.pmid: 21154909
references.citation: Neuroscience. 2008 Oct 2;156(2):374-80
references.pmid: 18713646
references.citation: Hear Res. 1999 Aug;134(1-2):77-88
references.pmid: 10452378
references.citation: Neuron. 2013 Jan 9;77(1):155-67
references.pmid: 23312523
references.citation: Neuroscience. 2007 Mar 16;145(2):715-26
references.pmid: 17275194
references.citation: Rev Neurosci. 2003;14(3):285-301
references.pmid: 14513869
references.citation: Hear Res. 1993 Jun;68(1):131-41
references.pmid: 8376210
references.citation: J Neurosci. 2013 Aug 21;33(34):13713-23
references.pmid: 23966693
references.citation: J Neurophysiol. 2003 Nov;90(5):2987-3000
references.pmid: 12815025
references.citation: J Neurosci. 1997 May 15;17(10):3766-77
references.pmid: 9133396
references.citation: Nat Neurosci. 1998 Aug;1(4):279-85
references.pmid: 10195160
references.citation: J Neurophysiol. 2008 Aug;100(2):646-56
references.pmid: 18525018
references.citation: J Neurosci. 2001 Sep 15;21(18):7303-12
references.pmid: 11549740
references.citation: Eur J Neurosci. 2010 May;31(9):1616-28
references.pmid: 20525074
references.citation: J Neurosci Methods. 1996 Apr;65(2):113-36
references.pmid: 8740589
references.citation: Eur J Neurosci. 2010 Jul;32(1):155-64
references.pmid: 20597969
references.citation: Neuron. 2011 Oct 20;72(2):231-43
references.pmid: 22017986
references.citation: J Comp Physiol A. 1997 Dec;181(6):559-71
references.pmid: 9449817
references.citation: Acta Otolaryngol. 2000 Sep;120(6):750-6
references.pmid: 11099153
references.citation: J Comp Neurol. 1999 Mar 29;406(1):72-86
references.pmid: 10100893
references.citation: J Neurosci. 1999 Dec 1;19(23):10451-60
references.pmid: 10575042
references.citation: J Neurosci. 2009 Nov 11;29(45):14077-85
references.pmid: 19906956
references.citation: Nat Neurosci. 2007 Jun;10(6):743-53
references.pmid: 17515899
references.citation: Hear Res. 2011 Sep;279(1-2):60-6
references.pmid: 21586318
references.citation: Acta Otolaryngol Suppl. 2007 Oct;(558):73-7
references.pmid: 17882574
references.citation: Audiology. 1972 Jan-Apr;11(1):97-114
references.pmid: 4669915
references.citation: Neuroimage. 2011 Jul 1;57(1):190-7
references.pmid: 21530663
references.citation: Curr Biol. 2007 Jul 3;17(13):R496-500
references.pmid: 17610826
references.citation: Front Neuroanat. 2014 Jun 02;8:40
references.pmid: 24917792
references.citation: Nat Neurosci. 2013 Aug;16(8):1068-76
references.pmid: 23817549
references.citation: PLoS One. 2009;4(7):e6099
references.pmid: 19584920
references.citation: J Gen Physiol. 2011 Sep;138(3):311-20
references.pmid: 21875980
references.citation: Nature. 2012 Apr 26;484(7395):473-8
references.pmid: 22538608
references.citation: Neuron. 2013 Dec 4;80(5):1218-31
references.pmid: 24239124
references.citation: Nat Neurosci. 2015 Feb;18(2):170-81
references.pmid: 25622573
references.citation: Audiol Neurootol. 1997 Sep-Oct;2(5):257-80
references.pmid: 9390836
references.citation: Hear Res. 2005 Aug;206(1-2):177-84
references.pmid: 16081007
references.citation: Hear Res. 2003 Sep;183(1-2):137-53
references.pmid: 13679145
references.citation: Nature. 2014 Jan 16;505(7483):318-26
references.pmid: 24429630
references.citation: Nat Methods. 2010 May;7(5):399-405
references.pmid: 20400966
references.citation: Neuron. 2014 Aug 20;83(4):944-59
references.pmid: 25088366
references.citation: J Neurophysiol. 2009 Dec;102(6):3251-9
references.pmid: 19776361
references.citation: Hear Res. 1999 Sep;135(1-2):146-62
references.pmid: 10491963
references.citation: Neurosci Res. 1994 May;19(3):303-16
references.pmid: 8058206
references.citation: Hear Res. 2011 Jun;276(1-2):61-9
references.pmid: 21056098
references.citation: Neuron. 2007 Mar 1;53(5):735-46
references.pmid: 17329212
references.citation: Neurosci Biobehav Rev. 2011 Apr;35(5):1089-109
references.pmid: 21094182
references.citation: J Neurophysiol. 2013 Jul;110(1):243-56
references.pmid: 23576700
references.citation: Eur J Neurosci. 2006 Nov;24(9):2515-29
references.pmid: 17100840
references.citation: Nat Neurosci. 2007 Dec;10(12):1594-600
references.pmid: 17994013
references.citation: Hear Res. 1987;26(3):239-47
references.pmid: 3583925
references.citation: J Neurophysiol. 2005 Jan;93(1):557-69
references.pmid: 15456804
references.citation: Physiology (Bethesda). 2007 Dec;22:358-65
references.pmid: 18073408
references.citation: Hear Res. 1980 Jul;3(1):3-16
references.pmid: 7400047
references.citation: Hear Res. 2011 Sep;279(1-2):111-7
references.pmid: 21527325
references.citation: J Neurosci. 2007 Sep 5;27(36):9711-20
references.pmid: 17804631
references.citation: Neuroscience. 2010 Apr 14;166(4):1194-209
references.pmid: 20096752