title: Electrospun mat with eyelid fat-derived stem cells as a scaffold for ocular epithelial regeneration.
abstract: The aim of this study was to develop nanofibrous gelatin substrates for eyelid fat stem cell (EFSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. Biocompatibility results indicated that all substrates were highly biocompatible, as EFSCs could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the EFSC were firmly anchored to the substrates and were able to retain a normal stem cell phenotype. Immunocytochemistry (ICC) and real time-PCR results revealed change in the expression profile of EFSCs grown on nanofibrous substrates when compared to those grown on control in epithelial induction condition. In addition, electrospun gelatin mats especially oriented scaffold provides not only a milieu supporting EFSCs expansion, but also serves as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane.
authors.affiliation: a Brain and Spinal Injury Research Center, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Daruosh
authors.initials: D
authors.lastname: Momenzadeh
authors.affiliation: b Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Baradaran-Rafii
authors.affiliation: c Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Saeed Heidari
authors.initials: SH
authors.lastname: Keshel
authors.affiliation: d Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ebrahimi
authors.affiliation: e Department of Biomaterials Engineering , Tonekabon Branch, Islamic Azad University , Tonekabon , Iran.
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Biazar
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D003316:Corneal Diseases / Q000628:therapy*; D005143:Eyelids / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D012038:Regeneration; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: Cellular analyses; eyelid fat stem cells; gelatin; nanofibrous scaffold; orientation