title: The importance of 2D aggregates on the antimicrobial resistance of Staphylococcus aureus sessile bacteria.
abstract: Biofilms formed on implanted devices are difficult to eradicate. Adhesion mechanism, high bacterial density, aggregation, induction of persisters and stressed bacteria are some of the factors considered when the antimicrobial resistance of these biofilms is analyzed. The aim of this work was to provide an alternative approach to the understanding of this issue by using a specially designed experimental set up that includes the use of microstructured (MS) surfaces (potential inhibitors of bacterial aggregation) in combination with antimicrobial agents (streptomycin and levofloxacin) against Staphylococcusaureus attached cells. Biofilms formed on smooth surfaces were used as plain controls (biofilmed-PC) characterized by the formation of dense 2D bacterial aggregates. Results showed bacterial persistence when streptomycin or levofloxacin were applied to PC-biofilms. The antimicrobial activity of both antibiotics was enhanced when bacteria were attached on MS, where single cells or small aggregates were observed. Thus, dense 2D aggregates of bacteria seem to be crucial as a required previous stage to develop the antimicrobial resistance.
authors.affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICET-Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, Sucursal 4, 1900 La Plata, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Miñán
authors.affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICET-Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, Sucursal 4, 1900 La Plata, Argentina. Electronic address: pls@inifta.unlp.edu.ar.
authors.forename: P L
authors.initials: PL
authors.lastname: Schilardi
authors.affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICET-Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, Sucursal 4, 1900 La Plata, Argentina; Facultad de Ingeniería, Universidad Nacional de La Plata, Calle 47 y 1, 1900 La Plata, Argentina. Electronic address: mmele@inifta.unlp.edu.ar.
authors.forename: M
authors.initials: M
authors.lastname: Fernández Lorenzo de Mele
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D064704:Levofloxacin / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000502:physiology*; D013307:Streptomycin / Q000737:chemistry / Q000494:pharmacology
chemical_list: D064704:Levofloxacin; D013307:Streptomycin
keywords: Antibiotic; Bacterial density; Biofilm; Persister; Staphyloccocus; Surface topography