title: Vaccination with DNA Encoding Truncated Enterohemorrhagic Escherichia coli (EHEC) Factor for Adherence-1 Gene (efa-1') Confers Protective Immunity to Mice Infected with E. coli O157:H7.
abstract: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is the predominant causative agent of hemorrhagic colitis in humans and is the cause of haemolytic uraemic syndrome and other illnesses. Cattle have been implicated as the main reservoir of this organism. Here, we evaluated the immunogenicity and protective efficacy of a DNA vaccine encoding conserved sequences of truncated EHEC factor for adherence-1 (efa-1') in a mouse model. Intranasal administration of plasmid DNA carrying the efa-1' gene (pVAXefa-1') into C57BL/6 mice elicited both humoral and cellular immune responses. In animals immunized with pVAXefa-1', EHEC-secreted protein-specific IgM and IgG antibodies were detected in sera at day 45. Anti-EHEC-secreted protein sIgA was also detected in nasal and bronchoalveolar lavages. In addition, antigen-specific T-cell-proliferation, IL-10, and IFN-γ were observed upon re-stimulation with either heat-killed bacteria or EHEC-secreted proteins. Vaccinated animals were also protected against challenge with E. coli O157:H7 strain EDL933. These results suggest that DNA vaccine encoding efa-1' have therapeutic potential in interventions against EHEC infections. This approach could lead to a new strategy in the production of vaccines that prevent infections in cattle.
authors.affiliation: Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Roberto
authors.initials: R
authors.lastname: Riquelme-Neira
authors.affiliation: Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Rivera
authors.affiliation: Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Darwin
authors.initials: D
authors.lastname: Sáez
authors.affiliation: Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Fernández
authors.affiliation: Microbiology and Mycology Program, Faculty of Medicine, Institute of Biomedical Sciences, University of Chile Santiago, Chile.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Osorio
authors.affiliation: Microbiology and Mycology Program, Faculty of Medicine, Institute of Biomedical Sciences, University of Chile Santiago, Chile.
authors.forename: Felipe
authors.initials: F
authors.lastname: del Canto
authors.affiliation: Microbiology and Mycology Program, Faculty of Medicine, Institute of Biomedical Sciences, University of Chile Santiago, Chile.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Salazar
authors.affiliation: Microbiology and Mycology Program, Faculty of Medicine, Institute of Biomedical Sciences, University of Chile Santiago, Chile.
authors.forename: Roberto M
authors.initials: RM
authors.lastname: Vidal
authors.affiliation: Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Angel
authors.initials: A
authors.lastname: Oñate
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D058491:Bacterial Load; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control*; D019453:Escherichia coli O157 / Q000235:genetics / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D008810:Mice, Inbred C57BL; D009296:Nasal Cavity / Q000737:chemistry; D010957:Plasmids; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; C404907:Efa1 protein, E coli; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D019444:Vaccines, DNA; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: DNA vaccine; O157:H7 serotype; enterohemorragic E. coli; protective to mice; truncated efa-1 gene
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