title: Quercetin-modulated erythrocyte membrane sodium-hydrogen exchanger during human aging: correlation with ATPase's.
abstract: BACKGROUND
  Quercetin uptake by erythrocytes is rapid. The sodium-hydrogen exchanger (NHE) is a secondary active transporter, regulating intracellular pH, Na(+) concentration and cell volume.
  
  
  OBJECTIVE
  The aim of present study was to investigate NHE as a function of human age and effect of quercetin on its activity. The NHE activity was correlated with erythrocytes ATPases.
  
  
  METHODS
  We analyzed normal, healthy subjects of both sexes (20-82 years). NHE activity was estimated in terms of amiloride-sensitive H+-efflux from acid-loaded cells.
  
  
  RESULTS
  A significant age-dependent increase in NHE activity was observed during aging in humans. Concentration (10(-3 )M to 10(-8 )M)-dependent in vitro treatment with quercetin causes inhibition of NHE activity. The Na(+)/K(+) -ATPase (r = 0.8882) and Ca(2+)-ATPase (r = 0.9540) activities positively correlated with it.
  
  
  CONCLUSIONS
  The present data show an additional mechanism where dietary flavonoids may exerts beneficial effect during aging.
authors.affiliation: a Amity Institute of Biotechnology, Amity University Uttar Pradesh , Noida , India .
authors.forename: Prabhanshu
authors.initials: P
authors.lastname: Kumar
authors.affiliation: b Department of Biochemical Engineering & Biotechnology , Indian Institute of Technology , Delhi , India , and.
authors.forename: Subhash
authors.initials: S
authors.lastname: Chand
authors.affiliation: a Amity Institute of Biotechnology, Amity University Uttar Pradesh , Noida , India .|c Department of Psychiatry , Interdisciplinary Laboratory of Clinical Neuroscience (LINC), Federal University of São Paulo , São Paulo , Brazil.
authors.forename: Pawan Kumar
authors.initials: PK
authors.lastname: Maurya
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011794:Quercetin / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017923:Sodium-Hydrogen Exchangers; D011794:Quercetin; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Aging; Ca2+-ATPase; Na+/H+ exchanger; Na+/K+ -ATPase; quercetin