title: Ectopic expression of phloem motor protein pea forisome PsSEO-F1 enhances salinity stress tolerance in tobacco.
abstract: PsSEOF-1 binds to calcium and its expression is upregulated by salinity treatment. PsSEOF - 1 -overexpressing transgenic tobacco showed enhanced salinity stress tolerance by maintaining cellular ion homeostasis and modulating ROS-scavenging pathway. Calcium (Ca(2+)) plays important role in growth, development and stress tolerance in plants. Cellular Ca(2+) homeostasis is achieved by the collective action of channels, pumps, antiporters and by Ca(2+) chelators present in the cell like calcium-binding proteins. Forisomes are ATP-independent mechanically active motor proteins known to function in wound sealing of injured sieve elements of phloem tissue. The Ca(2+)-binding activity of forisome and its role in abiotic stress signaling were largely unknown. Here we report the Ca(2+)-binding activity of pea forisome (PsSEO-F1) and its novel function in promoting salinity tolerance in transgenic tobacco. Native PsSEO-F1 promoter positively responded in salinity stress as confirmed using GUS reporter. Overexpression of PsSEO-F1 tobacco plants confers salinity tolerance by alleviating ionic toxicity and increased ROS scavenging activity which probably results in reduced membrane damage and improved yield under salinity stress. Evaluation of several physiological indices shows an increase in relative water content, electrolyte leakage, proline accumulation and chlorophyll content in transgenic lines as compared with null-segregant control. Expression of several genes involved in cellular homeostasis is perturbed by PsSEO-F1 overexpression. These findings suggest that PsSEO-F1 provides salinity tolerance through cellular Ca(2+) homeostasis which in turn modulates ROS machinery providing indirect link between Ca(2+) and ROS signaling under salinity-induced perturbation. PsSEO-F1 most likely functions in salinity stress tolerance by improving antioxidant machinery and mitigating ion toxicity in transgenic lines. This finding should make an important contribution in our better understanding of the significance of calcium signaling in phloem tissue leading to salinity stress tolerance.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Vineet Kumar
authors.initials: VK
authors.lastname: Srivastava
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Shailendra
authors.initials: S
authors.lastname: Raikwar
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Tuteja
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India. narendra@icgeb.res.in.|Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Sector 125, Noida, Uttar Pradesh, 201313, India. narendra@icgeb.res.in.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000066630:Ectopic Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006706:Homeostasis; D018532:Pisum sativum / Q000235:genetics*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D054712:Salinity; D055049:Salt Tolerance; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
chemical_list: D002135:Calcium-Binding Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D012965:Sodium Chloride; D002118:Calcium
keywords: ATP-independent motor protein; Calcium sensor; Pea forisome; Pisum sativum; ROS; Salinity stress adaptation
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