title: The late and dual origin of cerebrospinal fluid-contacting neurons in the mouse spinal cord.
abstract: Considerable progress has been made in understanding the mechanisms that control the production of specialized neuronal types. However, how the timing of differentiation contributes to neuronal diversity in the developing spinal cord is still a pending question. In this study, we show that cerebrospinal fluid-contacting neurons (CSF-cNs), an anatomically discrete cell type of the ependymal area, originate from surprisingly late neurogenic events in the ventral spinal cord. CSF-cNs are identified by the expression of the transcription factors Gata2 and Gata3, and the ionic channels Pkd2l1 and Pkd1l2. Contrasting with Gata2/3(+) V2b interneurons, differentiation of CSF-cNs is independent of Foxn4 and takes place during advanced developmental stages previously assumed to be exclusively gliogenic. CSF-cNs are produced from two distinct dorsoventral regions of the mouse spinal cord. Most CSF-cNs derive from progenitors circumscribed to the late-p2 and the oligodendrogenic (pOL) domains, whereas a second subset of CSF-cNs arises from cells bordering the floor plate. The development of these two subgroups of CSF-cNs is differentially controlled by Pax6, they adopt separate locations around the postnatal central canal and they display electrophysiological differences. Our results highlight that spatiotemporal mechanisms are instrumental in creating neural cell diversity in the ventral spinal cord to produce distinct classes of interneurons, motoneurons, CSF-cNs, glial cells and ependymal cells.
authors.affiliation: Developmental Neurobiology Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.
authors.forename: Yanina L
authors.initials: YL
authors.lastname: Petracca
authors.affiliation: Developmental Neurobiology Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.
authors.forename: Maria Micaela
authors.initials: MM
authors.lastname: Sartoretti
authors.affiliation: Developmental Neurobiology Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.
authors.forename: Daniela J
authors.initials: DJ
authors.lastname: Di Bella
authors.affiliation: Neuronal Plasticity Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.
authors.forename: Antonia
authors.initials: A
authors.lastname: Marin-Burgin
authors.affiliation: Developmental Neurobiology Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.
authors.forename: Abel L
authors.initials: AL
authors.lastname: Carcagno
authors.affiliation: Neuronal Plasticity Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.
authors.forename: Alejandro F
authors.initials: AF
authors.lastname: Schinder
authors.affiliation: Developmental Neurobiology Lab, Instituto Leloir and Consejo Nacional de Investigaciones Científicas y Técnicas (IIBBA-CONICET), Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina GLanuza@Leloir.org.ar.
authors.forename: Guillermo M
authors.initials: GM
authors.lastname: Lanuza
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D004594:Electrophysiology; D005136:Eye Proteins / Q000235:genetics; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics; D050989:GATA2 Transcription Factor / Q000235:genetics; D005838:Genotype; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007395:Interneurons / Q000166:cytology; D051379:Mice; D009046:Motor Neurons / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D013116:Spinal Cord / Q000196:embryology* / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology
chemical_list: D005136:Eye Proteins; D051858:Forkhead Transcription Factors; C438622:Foxn4 protein, mouse; D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse
keywords: Central canal; Ependyma; Late-born neurons; Spinal cord; Transcription factor
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