title: A novel bispecific antibody, BiSS, with potent anti-cancer activities.
abstract: One of the most active fields in cancer immunotherapy is the study of bispecific antibodies, which engage immune cells to kill cancer cells. However, a variety of issues are associated with most of current bispecific antibody formats. In this study, we present a novel bispecific antibody, BiSS (Bispecific antibody with Single domain, Single domain antibodies), which was constructed by linking 2 single domain antibodies, anti-CEA and anti-CD16, in tandem. Unlike most other bispecific antibodies, the BiSS antibody can be expressed and purified from E.coli in large quantities. By recruiting natural killer cells (NK cells) to CEA-positive cancer cells, BiSS led to cancer cell death in vitro. In xenograft models, the BiSS protein blocked cancer progression. The data suggested that the single domain-based bispecific antibody BiSS was functional and can be potentially applied to a broad range of immunotherapies.
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Changhua
authors.initials: C
authors.lastname: Zhou
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Chen
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Ji
authors.initials: J
authors.lastname: Miao
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou , China.|b Centre for Cellular & Structural Biology , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D018033:Antibodies, Bispecific / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007694:Killer Cells, Natural / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D023041:Xenograft Model Antitumor Assays
chemical_list: D018033:Antibodies, Bispecific
keywords: Bacterial expression; BiSS; CD16; CEA; bispecific antibody; natural killer cells; single domain antibody
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