title: Metal ion transport quantified by ICP-MS in intact cells.
abstract: The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions.
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, OH USA.|University of Cincinnati/Agilent Technologies Metallomics Center of the Americas, Cincinnati, OH USA.
authors.forename: Julio A Landero
authors.initials: JA
authors.lastname: Figueroa
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, OH USA.|University of Cincinnati/Agilent Technologies Metallomics Center of the Americas, Cincinnati, OH USA.
authors.forename: Cory A
authors.initials: CA
authors.lastname: Stiner
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH USA.
authors.forename: Tatiana L
authors.initials: TL
authors.lastname: Radzyukevich
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH USA.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Heiny
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D016623:Ion Pumps / Q000378:metabolism; D017136:Ion Transport; D007477:Ions / Q000378:metabolism*; D007962:Leukocytes / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008670:Metals / Q000378:metabolism*; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry*; D011188:Potassium / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism
chemical_list: D016623:Ion Pumps; D007477:Ions; D008670:Metals; D013455:Sulfur; D011188:Potassium
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