title: Bacterial composition in whole saliva from patients with severe hyposalivation--a case-control study.
abstract: OBJECTIVE
  The purpose of this study was to compare the microbiota of stimulated whole saliva samples from patients with severe hyposalivation to samples from individuals with normal whole saliva flow rates. It was hypothesized that the two groups differ with regard to salivary bacterial profiles.
  
  
  METHODS
  This cross-sectional study included 36 participants (24 females and 12 males, mean age 58.5 years) with severe hyposalivation and 36 gender-, age-, and geographically matched participants with normal salivary secretion from the Danish Health Examination Survey (DANHES). The microbiota of stimulated whole saliva samples was characterized by HOMINGS.
  
  
  RESULTS
  The two groups had comparable caries experience measured by decayed, missed, filled surfaces/teeth and decayed, missed, filled root surfaces as well as active caries lesions. In addition, no single probe target was present with a significant difference in frequency or proportional presence between groups. Furthermore, data reduction by principal component analysis and correspondence analysis showed comparable bacterial community profiles between groups.
  
  
  CONCLUSIONS
  The results indicate that the salivary bacterial profiles of patients with severe hyposalivation do not differ from those of individuals with normal salivary secretion, when there are virtually no untreated active caries lesions present in the oral cavity.
authors.affiliation: Section 1, Periodontology, Oral Microbiology, and Community Dentistry, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Belstrøm
authors.affiliation: Section 1, Periodontology, Oral Microbiology, and Community Dentistry, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Holmstrup
authors.affiliation: Department of Immunology & Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: N-E
authors.initials: NE
authors.lastname: Fiehn
authors.affiliation: Section 1, Periodontology, Oral Microbiology, and Community Dentistry, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Rosing
authors.affiliation: Section 1, Oral Medicine, Clinical Oral Physiology, Oral Anatomy and Pathology, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Bardow
authors.affiliation: Department of Microbiology, The Forsyth Institute, Cambridge, MA, USA.|Department of Oral Medicine, Infection & Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Paster
authors.affiliation: Section 1, Oral Medicine, Clinical Oral Physiology, Oral Anatomy and Pathology, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: A M
authors.initials: AM
authors.lastname: Lynge Pedersen
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D064307:Microbiota; D008875:Middle Aged; D012463:Saliva / Q000382:microbiology*; D014987:Xerostomia / Q000382:microbiology*
keywords: Human Oral Microbe Identification using Next Generation Sequencing; bacteria; dental caries; hyposalivation; saliva