title: Characterization of Nonribosomal Peptide Synthetases with NRPSsp.
abstract: Bioinformatic sequence analysis allows the functional characterization of newly sequenced proteins. Nonribosomal peptide synthetases (NRPSs) are multi-modular enzymes involved in the biosynthesis of natural products. The current omics era has enabled the exponential growth of the sequenced NRPS, and it is important to characterize the final product of these synthetases. Here, how to achieve the prediction of substrates which bind to adenylation domains in NRPS with NRPSsp (www.nrpssp.com) bioinformatic tool is described.
authors.affiliation: Bioinformatics Service, Nucleus, University of Salamanca (USAL), Edificio I+D+i, C/ Espejo 2, Salamanca, 37007, Spain. cprietos@usal.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Prieto
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D020407:Internet; D008954:Models, Biological; D010453:Peptide Synthases / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods*; D012984:Software; D013379:Substrate Specificity
chemical_list: D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Bioinformatics; Classifier; NRPSsp; Nonribosomal; Peptide; Synthetases