title: Characterization and expression analysis of Lc-Sox4 in large yellow croaker Larimichthys crocea.
abstract: The characterization and expression of Sox4 in large yellow croaker (Lc-Sox4) were studied in this paper. Lc-Sox4 contains a protein of 371 amino acids with a conserved high mobility group box. Quantitative real-time PCR displayed that the expression of Lc-Sox4 had tissue and gender specificity existing in brain, gonad, heart, intestine, and head kidney with male>female, in eye with female>male. During embryogenesis, Lc-Sox4 was expressed highest in one-day-post-hatching stage, next in formation-of-eye-lens stage. The expression pattern of Lc-Sox4 was different from that of Lc-Sox11a. The expression of Lc-Sox4 was significantly lower than that of Lc-Sox11a in the all tested tissues and embryonic stages except in heart, spleen, mutiple-cell, formation-of-eye-lens, and one-day-post-hatching stages (with Lc-Sox4 higher than Lc-Sox11a). There was overlapping expression between Lc-Sox4 and Lc-Sox11a in brain, gill, female eye, testis, formation-of-eye-lens stage and one-day post hatching stage. The whole mount in situ hybridization results indicated that Lc-Sox4 was expressed at all embryonic stages except 2-cell stage. The positive signals were mainly distributed in the central nervous system and notochord at one-day-post-hatching stage. In short, we first identified and analyzed the temporal and spatial expression patterns of Lc-Sox4 to elucidate its important influence on the development of nervous system, visual system and heart. We also detected the overlapping expression between Lc-Sox4 and Lc-Sox11a which may reveal the functional redundancy of them. These data would shed light on the molecular mechanism of development in large yellow croaker.
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China; State Key Laboratory of Large Yellow Croaker Breeding, Ningde Fufa Fisheries Company Limited, Ningde 352103, China.
authors.forename: Kunhuang
authors.initials: K
authors.lastname: Han
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China; State Key Laboratory of Large Yellow Croaker Breeding, Ningde Fufa Fisheries Company Limited, Ningde 352103, China. Electronic address: ylwang@jmu.edu.cn.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: zhangziping@hotmail.com.
authors.forename: Ziping
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008958:Models, Molecular; D010473:Perciformes / Q000196:embryology / Q000235:genetics*; D011487:Protein Conformation; D021381:Protein Transport; D055751:SOXC Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D029941:Fish Proteins; D055751:SOXC Transcription Factors
keywords: Embryogenesis; Expression patterns; Larimichthys crocea; Lc-Sox4; WISH; qRT-PCR