title: A novel role for the deubiquitinase USP1 in the control of centrosome duplication.
abstract: Defects in the regulation of centrosome duplication lead to tumorigenesis through abnormal cell division and resulting chromosome missegregation. Therefore, maintenance of accurate centrosome number is critical for cell fate. The deubiquitinating enzyme USP1 plays important roles in DNA repair and cell differentiation. Importantly, increased levels of USP1 are detected in certain types of human cancer, but little is known about the significance of USP1 overexpression in cancer development. Here we show that Usp1 plays a novel role in regulating centrosome duplication. The ectopic expression of wild-type Usp1, but not C90S Usp1 (catalytically inactive mutant form), induced centrosome amplification. Conversely, ablation of Usp1 in mouse embryonic fibroblasts (MEFs) showed a significant delay in centrosome duplication. Moreover, Usp1-induced centrosome amplification caused abnormal mitotic spindles, chromosome missegregation and aneuploidy. Interestingly, loss of inhibitor of DNA binding protein 1 (ID1) suppressed Usp1-induced centrosome amplification. Taken together, our results strongly suggest that Usp1 is involved in the regulation of centrosome duplication, at least in part via ID1, and Usp1 may exert its oncogenic activity, partially through inducing centrosome abnormality.
authors.affiliation: a Department of Pharmacology , Medical Research Center for Gene Regulation, Chonnam National University Medical School , Gwangju , Korea.
authors.forename: Jin Ki
authors.initials: JK
authors.lastname: Jung
authors.affiliation: a Department of Pharmacology , Medical Research Center for Gene Regulation, Chonnam National University Medical School , Gwangju , Korea.
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Jang
authors.affiliation: a Department of Pharmacology , Medical Research Center for Gene Regulation, Chonnam National University Medical School , Gwangju , Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Kim
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D018385:Centrosome / Q000378:metabolism*; D020090:Chromosome Segregation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000235:genetics*; D008941:Spindle Apparatus / Q000235:genetics; D064570:Ubiquitin-Specific Proteases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C497455:Idb1 protein, mouse; D051798:Inhibitor of Differentiation Protein 1; D064570:Ubiquitin-Specific Proteases; C579227:Usp1 protein, mouse
keywords: Aneuploidy; Centrosome amplification; Chromosome instability; Deubiquitinating Enzymes; Usp1
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