title: Lipid Lowering Effects of Hydroalcoholic Extract of Anethum graveolens L. and Dill Tablet in High Cholesterol Fed Hamsters.
abstract: Objective. This study was aimed to determine the effect of Anethum graveolens extract and Anethum graveolens (dill) tablet on lipid profile, liver enzymes, and gene expression and enzymatic activity of HMG-CoA reductase in high cholesterol fed hamsters. Materials and Methods. Golden Syrian male hamsters (130 ± 10 g) were randomly divided into 6 groups (n = 6) and received daily the following: group 1 received chow + 2% cholesterol + 0.5% cholic acid (HCD), groups 2 and 3 received HCD diet plus 100 and 200 mg/kg hydroalcoholic extract of dill, respectively, and groups 4 and 5 received HCD diet plus 100 and 200 mg/kg dill tablet, respectively. Group 6 received only chow. After 1 month feeding serum biochemical factors were determined. HMG-CoA reductase mRNA level was measured (real-time PCR) and its activity was determined spectrophotometrically. Results. Compared with hypercholesterolemic group 1, lipid profile, blood glucose, and liver enzymes significantly decreased in all dill tablet or dill extract treated groups (p < 0.05). The changes in HMG-CoA reductase gene expression level and enzyme activity significantly reduced in animals that received 200 mg/kg of extract or tablet. Conclusion. Dill extract and dill tablet showed potential hypocholesterolemic properties in hamsters by inhibition of HMG-CoA reductase activity.
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Abbasi Oshaghi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Khodadadi
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Yadegarazari
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Shabab
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Heidar
authors.initials: H
authors.lastname: Tavilani
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran; Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
authors.forename: Mohamad Taghi
authors.initials: MT
authors.lastname: Goodarzi
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