title: Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool.
abstract: Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hoenen
authors.forename: Allison
authors.initials: A
authors.lastname: Groseth
authors.forename: Kyle
authors.initials: K
authors.lastname: Rosenke
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Fischer
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoenen
authors.forename: Seth D
authors.initials: SD
authors.lastname: Judson
authors.forename: Cynthia
authors.initials: C
authors.lastname: Martellaro
authors.forename: Darryl
authors.initials: D
authors.lastname: Falzarano
authors.forename: Andrea
authors.initials: A
authors.lastname: Marzi
authors.forename: R Burke
authors.initials: RB
authors.lastname: Squires
authors.forename: Kurt R
authors.initials: KR
authors.lastname: Wollenberg
authors.forename: Emmie
authors.initials: E
authors.lastname: de Wit
authors.forename: Joseph
authors.initials: J
authors.lastname: Prescott
authors.forename: David
authors.initials: D
authors.lastname: Safronetz
authors.forename: Neeltje
authors.initials: N
authors.lastname: van Doremalen
authors.forename: Trenton
authors.initials: T
authors.lastname: Bushmaker
authors.forename: Friederike
authors.initials: F
authors.lastname: Feldmann
authors.forename: Kristin
authors.initials: K
authors.lastname: McNally
authors.forename: Fatorma K
authors.initials: FK
authors.lastname: Bolay
authors.forename: Barry
authors.initials: B
authors.lastname: Fields
authors.forename: Tara
authors.initials: T
authors.lastname: Sealy
authors.forename: Mark
authors.initials: M
authors.lastname: Rayfield
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Nichol
authors.forename: Kathryn C
authors.initials: KC
authors.lastname: Zoon
authors.forename: Moses
authors.initials: M
authors.lastname: Massaquoi
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Munster
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
mesh_terms: D004196:Disease Outbreaks; D029043:Ebolavirus / Q000235:genetics*; D016679:Genome, Viral; D019142:Hemorrhagic Fever, Ebola / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D009154:Mutation; D058608:Nanopores; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: DNA; Ebola hemorrhagic fever; Ebola virus; Ebola virus disease; Ebolavirus; Liberia; West Africa; disease outbreaks; high-throughput nucleotide sequencing; molecular diagnostic techniques; nanopore sequencing; viruses
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