title: Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India.
abstract: BACKGROUND
  Rabies poses a serious public health concern in developing countries such as India.
  
  
  OBJECTIVE
  The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India.
  
  
  METHODS
  Nucleoprotein gene from eight (of total 20 suspected samples) rabies cases that tested positive for rabies antigen using reverse transcriptase-polymerase chain reaction (RT-PCR) were sequenced.
  
  
  RESULTS
  Sequence analysis using basic local alignment search tool (BLAST) and multiple sequence alignment (MSA) and phylogenetic analysis showed similarity to previously reported sequences from India and those of Arctic lineages.
  
  
  CONCLUSIONS
  The circulating RABV strains in Maharashtra, India show genetic relatedness to RABV strains reported from Indo-Arctic lineages and India-South and Japan.
authors.forename: S
authors.initials: S
authors.lastname: Mehta
authors.affiliation: Department of Virology and Immunology, Haffkine Institute for Training, Research and Testing, Mumbai, Maharashtra, India.
authors.forename: P
authors.initials: P
authors.lastname: Charan
authors.forename: R
authors.initials: R
authors.lastname: Dahake
authors.forename: S
authors.initials: S
authors.lastname: Mukherjee
authors.forename: A
authors.initials: A
authors.lastname: Chowdhary
mesh_terms: D006801:Humans; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D009698:Nucleoproteins; D012367:RNA, Viral
references.citation: J Clin Microbiol. 2000 Feb;38(2):755-62
references.pmid: 10655381
references.citation: Epidemiol Infect. 2002 Jun;128(3):503-11
references.pmid: 12113496
references.citation: Acta Virol. 2004;48(1):47-50
references.pmid: 15230475
references.citation: J Clin Virol. 2006 May;36(1):17-23
references.pmid: 16504574
references.citation: Int J Infect Dis. 2007 Jan;11(1):29-35
references.pmid: 16678463
references.citation: J Clin Microbiol. 2006 Sep;44(9):3218-24
references.pmid: 16954251
references.citation: Emerg Infect Dis. 2007 Jan;13(1):111-6
references.pmid: 17370523
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Emerg Infect Dis. 2008 Sep;14(9):1368-74
references.pmid: 18760002
references.citation: J Travel Med. 2008 Sep-Oct;15(5):372-4
references.pmid: 19006516
references.citation: Pathol Int. 2009 Aug;59(8):555-66
references.pmid: 19627539
references.citation: J Vet Sci. 2011 Mar;12(1):57-63
references.pmid: 21368564
references.citation: Virus Res. 2012 Jan;163(1):352-62
references.pmid: 22100340
references.citation: Virol Sin. 2012 Jun;27(3):194-203
references.pmid: 22684474
references.citation: Vector Borne Zoonotic Dis. 2013 May;13(5):324-9
references.pmid: 23473223
references.citation: World Health Organ Tech Rep Ser. 2013;(982):1-139, back cover
references.pmid: 24069724
references.citation: PLoS Negl Trop Dis. 2013 Nov 21;7(11):e2560
references.pmid: 24278494
references.citation: Bull World Health Organ. 2014 Apr 1;92(4):230
references.pmid: 24700986