title: Exposure of BALB/c Mice to Diesel Engine Exhaust Origin Secondary Organic Aerosol (DE-SOA) during the Developmental Stages Impairs the Social Behavior in Adult Life of the Males.
abstract: Secondary organic aerosol (SOA) is a component of particulate matter (PM) 2.5 and formed in the atmosphere by oxidation of volatile organic compounds. Recently, we have reported that inhalation exposure to diesel engine exhaust (DE) originated SOA (DE-SOA) affect novel object recognition ability and impair maternal behavior in adult mice. However, it is not clear whether early life exposure to SOA during the developmental stages affect social behavior in adult life or not. In the present study, to investigate the effects of early life exposure to DE-SOA during the gestational and lactation stages on the social behavior in the adult life, BALB/c mice were exposed to clean air (control), DE, DE-SOA and gas without any PM in the inhalation chambers from gestational day 14 to postnatal day 21 for 5 h a day and 5 days per week. Then adult mice were examined for changes in their social behavior at the age of 13 week by a sociability and social novelty preference, social interaction with a juvenile mouse and light-dark transition test, hypothalamic mRNA expression levels of social behavior-related genes, estrogen receptor-alpha and oxytocin receptor as well as of the oxidative stress marker gene, heme oxygenase (HO)-1 by real-time RT-PCR method. In addition, hypothalamic level of neuronal excitatory marker, glutamate was determined by ELISA method. We observed that sociability and social novelty preference as well as social interaction were remarkably impaired, expression levels of estrogen receptor-alpha, oxytocin receptor mRNAs were significantly decreased, expression levels of HO-1 mRNAs and glutamate levels were significantly increased in adult male mice exposed to DE-SOA compared to the control ones. Findings of this study indicate early life exposure of BALB/c mice to DE-SOA may affect their late-onset hypothalamic expression of social behavior related genes, trigger neurotoxicity and impair social behavior in the males.
authors.affiliation: Center for Environmental Health Sciences, National Institute for Environmental Studies Tsukuba, Japan.
authors.forename: Tin-Tin
authors.initials: TT
authors.lastname: Win-Shwe
authors.affiliation: Division of Life Science, Graduate School of Science and Engineering, Saitama University Saitama City, Japan.
authors.forename: Chaw
authors.initials: C
authors.lastname: Kyi-Tha-Thu
authors.affiliation: Division of Life Science, Graduate School of Science and Engineering, Saitama University Saitama City, Japan.
authors.forename: Yadanar
authors.initials: Y
authors.lastname: Moe
authors.affiliation: Center for Environmental Risk Research, National Institute for Environmental Studies Tsukuba, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujitani
authors.affiliation: Division of Life Science, Graduate School of Science and Engineering, Saitama University Saitama City, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Tsukahara
authors.affiliation: Center for Environmental Risk Research, National Institute for Environmental Studies Tsukuba, Japan.
authors.forename: Seishiro
authors.initials: S
authors.lastname: Hirano
keywords: brain; diesel exhaust; mice; secondary organic aerosol; social behavior
references.citation: Adv Exp Med Biol. 1999;468:69-80
references.pmid: 10635020
references.citation: Adv Exp Med Biol. 1999;468:81-95
references.pmid: 10635021
references.citation: Eur Respir J. 2001 Mar;17(3):428-35
references.pmid: 11405521
references.citation: JAMA. 2002 Mar 6;287(9):1132-41
references.pmid: 11879110
references.citation: Neuropeptides. 2002 Apr-Jun;36(2-3):221-9
references.pmid: 12359512
references.citation: Toxicol Pathol. 2004 Nov-Dec;32(6):650-8
references.pmid: 15513908
references.citation: Hum Mol Genet. 2005 Jan 15;14(2):205-20
references.pmid: 15548546
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16096-101
references.pmid: 16249339
references.citation: Toxicol Lett. 2006 May 25;163(2):153-60
references.pmid: 16293374
references.citation: Science. 2007 Mar 2;315(5816):1259-62
references.pmid: 17332409
references.citation: Neuropeptides. 2007 Jun;41(3):145-63
references.pmid: 17420046
references.citation: Toxicol Appl Pharmacol. 2008 Jan 15;226(2):192-8
references.pmid: 17950771
references.citation: Neurotoxicology. 2008 Jan;29(1):190-201
references.pmid: 18031821
references.citation: Toxicol Pathol. 2008 Feb;36(2):289-310
references.pmid: 18349428
references.citation: Brain Cogn. 2008 Nov;68(2):117-27
references.pmid: 18550243
references.citation: Neurotoxicology. 2008 Nov;29(6):940-7
references.pmid: 18926851
references.citation: Inhal Toxicol. 2009 Feb;21(3):200-9
references.pmid: 18991064
references.citation: Inhal Toxicol. 2009 Aug;21(10):828-36
references.pmid: 19653804
references.citation: Annu Rev Neurosci. 1990;13:171-82
references.pmid: 1970230
references.citation: Trends Neurosci. 2009 Sep;32(9):506-16
references.pmid: 19716187
references.citation: Environ Sci Technol. 2010 Feb 15;44(4):1424-30
references.pmid: 20092303
references.citation: Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4389-94
references.pmid: 20160081
references.citation: Behav Brain Res. 2010 Jul 11;210(2):211-20
references.pmid: 20184922
references.citation: Nature. 2010 Oct 14;467(7317):824-7
references.pmid: 20944744
references.citation: Arch Pharm Res. 2010 Oct;33(10):1539-56
references.pmid: 21052932
references.citation: Inhal Toxicol. 2011 Aug;23 Suppl 2:84-94
references.pmid: 21401387
references.citation: Nanotoxicology. 2012 Aug;6(5):543-53
references.pmid: 21663545
references.citation: Schizophr Res. 2011 Oct;132(1):50-3
references.pmid: 21840177
references.citation: Int J Mol Sci. 2011;12(9):6267-80
references.pmid: 22016657
references.citation: Brain Cogn. 2011 Dec;77(3):345-55
references.pmid: 22032805
references.citation: Environ Sci Technol. 2012 Jan 17;46(2):704-12
references.pmid: 22191732
references.citation: Indoor Air. 2012 Oct;22(5):415-26
references.pmid: 22372506
references.citation: J Toxicol. 2012;2012:782462
references.pmid: 22523490
references.citation: Environ Health Perspect. 2012 Jul;120(7):a258-60
references.pmid: 22543002
references.citation: Toxicol Appl Pharmacol. 2012 Aug 1;262(3):355-62
references.pmid: 22659509
references.citation: Behav Brain Res. 2013 Jan 1;236(1):23-9
references.pmid: 22944514
references.citation: Neurotoxicology. 2012 Oct;33(5):972-84
references.pmid: 22981845
references.citation: J Toxicol Sci. 2013 Feb;38(1):71-82
references.pmid: 23358141
references.citation: Schizophr Res. 2013 Jul;147(2-3):393-7
references.pmid: 23676253
references.citation: Front Neurosci. 2013 Oct 10;7:183
references.pmid: 24133408
references.citation: Biomed Res Int. 2014;2014:736385
references.pmid: 24524086
references.citation: Int J Environ Res Public Health. 2014 Oct 30;11(11):11286-307
references.pmid: 25361045
references.citation: Horm Behav. 2015 Aug;74:53-76
references.pmid: 26122289
references.citation: J Toxicol Sci. 2015;40(5):535-50
references.pmid: 26354370
references.citation: Curr Environ Health Rep. 2015 Dec;2(4):430-9
references.pmid: 26399256
references.citation: Nanomaterials (Basel). 2015 Jun 30;5(3):1147-1162
references.pmid: 28347057
references.citation: J Neurochem. 1984 Jan;42(1):1-11
references.pmid: 6139418
references.citation: FASEB J. 1993 Dec;7(15):1450-9
references.pmid: 7903261
references.citation: Environ Health Perspect. 1993 Dec;101 Suppl 4:187-91
references.pmid: 8206028
references.citation: Neuroreport. 1995 Nov 27;6(17):2357-62
references.pmid: 8747153
references.citation: Lancet. 1997 May 31;349(9065):1582-7
references.pmid: 9174559
references.citation: Brain Res. 1998 Sep 14;805(1-2):234-40
references.pmid: 9733972