title: Anti-Staphylococcal Biofilm Effects of Human Cathelicidin Peptides.
abstract: Staphylococcus aureus can live together in the form of biofilms to avoid elimination by the host. Thus, a useful strategy to counteract bacterial biofilms is to re-engineer human antimicrobial peptide LL-37 so that it can be used as a remedy for preventing and removing biofilms. This study reports antibiofilm effects of four human cathelicidin LL-37 peptides against community-associated and hospital isolated methicillin-resistant Staphylococcus aureus (MRSA) strains. Although the intact molecule LL-37 inhibited biofilm formation at low concentrations, it did not inhibit bacterial attachment nor disrupt preformed biofilms. However, two 17-residue peptides, GF-17 and 17BIPHE2, inhibited bacterial attachment, biofilm growth, and disrupted established biofilms. An inactive peptide RI-10 was used as a negative control. Our results obtained using the S. aureus mutants in a static biofilm model are consistent with the literature obtained in a flow cell biofilm model. Because 17BIPHE2 is the most effective biofilm disruptor with desired stability to proteases, it is a promising lead for developing new anti-MRSA biofilm agents.
authors.affiliation: Department of Microbiology and Pathology, University of Nebraska Medical Center , 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495, United States.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Mishra
authors.affiliation: Department of Microbiology and Pathology, University of Nebraska Medical Center , 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495, United States.
authors.forename: Radha M
authors.initials: RM
authors.lastname: Golla
authors.affiliation: Department of Microbiology and Pathology, University of Nebraska Medical Center , 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495, United States.
authors.forename: Kyle
authors.initials: K
authors.lastname: Lau
authors.affiliation: Department of Microbiology and Pathology, University of Nebraska Medical Center , 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495, United States.
authors.forename: Tamara
authors.initials: T
authors.lastname: Lushnikova
authors.affiliation: Department of Microbiology and Pathology, University of Nebraska Medical Center , 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495, United States.
authors.forename: Guangshun
authors.initials: G
authors.lastname: Wang
keywords: 17BIPHE2; Biofilms; GF-17; LL-37; Staphylococcus aureus; attachment mutants
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