title: Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants.
abstract: By use of a strategy incorporating the green fluorescent protein (GFP), facile and rapid monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two techniques for simple determination of virus titer in the baculovirus expression system using GFP co-expression and rapid monitoring of Sf-9 insect cell infection using a combination of GFP and the early-to-late (ETL) promoter of the virus vector. Because of its early appearance, GFP, when placed under the control of ETL promoter, will facilitate vector construction, virus isolation, and titer determination.
authors.affiliation: Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617, Taiwan.|Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA.
authors.forename: Hsuan-Chen
authors.initials: HC
authors.lastname: Wu
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 2226 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA. bentley@umd.edu.|Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA. bentley@umd.edu.
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D004274:DNA, Recombinant / Q000235:genetics; D023381:Endpoint Determination; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D061987:Sf9 Cells; D018411:Spodoptera; D019562:Viral Load / Q000379:methods*
chemical_list: D004274:DNA, Recombinant; D049452:Green Fluorescent Proteins
keywords: Baculovirus infection; Green fluorescent protein; Insect cell culture; Monitoring