title: Characterization of human follicular thyroid cancer cell lines in preclinical mouse models.
abstract: Follicular thyroid cancer (FTC) is the second most common type of thyroid cancers. In order to develop more effective personalized therapies, it is necessary to thoroughly evaluate patient-derived cell lines in in vivo preclinical models before using them to test new, targeted therapies. This study evaluates the tumorigenic and metastatic potential of a panel of three human FTC cell lines (WRO, FTC-238, and TT1609-CO2) with defined genetic mutations in two in vivo murine models: an orthotopic thyroid cancer model to study tumor progression and a tail vein injection model to study metastasis. All cell lines developed tumors in the orthotopic model, with take rates of 100%. Notably, WRO-derived tumors grew two to four times faster than tumors arising from the FTC-238 and TT2609-CO2 cell lines. These results mirrored those of a tail vein injection model for lung metastasis: one hundred percent of mice injected with WRO cells in the tail vein exhibited aggressive growth of bilateral lung metastases within 35 days. In contrast, tail vein injection of FTC-238 or TT2609-CO2 cells did not result in lung metastasis. Together, our work demonstrates that these human FTC cell lines display highly varied tumorigenic and metastatic potential in vivo with WRO being the most aggressive cell line in both orthotopic and lung metastasis models. This information will be valuable when selecting cell lines for preclinical drug testing.
authors.affiliation: Department of Otolaryngology, Head and Neck SurgerySaint Louis University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Ashley N
authors.initials: AN
authors.lastname: Reeb
authors.affiliation: Department of Otolaryngology, Head and Neck SurgerySaint Louis University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ziegler
authors.affiliation: Department of Otolaryngology, Head and Neck SurgerySaint Louis University School of Medicine, Saint Louis, Missouri, USA rlin7@slu.edu.
authors.forename: Reigh-Yi
authors.initials: RY
authors.lastname: Lin
keywords: follicular thyroid cancer; metastasis; mouse model; orthotopic; thyroid
references.citation: J Vis Exp. 2013 Apr 17;(74):null
references.pmid: 23628990
references.citation: J Clin Endocrinol Metab. 2010 Apr;95(4):1726-33
references.pmid: 20173019
references.citation: Sci Transl Med. 2012 Nov 7;4(159):159ra149
references.pmid: 23136044
references.citation: Thyroid. 2005 Sep;15(9):1011-20
references.pmid: 16187909
references.citation: Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6170-8
references.pmid: 17062694
references.citation: J Clin Endocrinol Metab. 2008 Nov;93(11):4331-41
references.pmid: 18713817
references.citation: Thyroid. 2014 Jul;24(7):1134-8
references.pmid: 24684373
references.citation: Nature. 2008 Dec 4;456(7222):593-8
references.pmid: 19052619
references.citation: BMC Cancer. 2005 Jul 19;5:80
references.pmid: 16029487
references.citation: PLoS One. 2011 Apr 29;6(4):e18382
references.pmid: 21559522
references.citation: Thyroid. 2001 Oct;11(10):909-17
references.pmid: 11716037
references.citation: Front Endocrinol (Lausanne). 2012 Nov 16;3:133
references.pmid: 23162534
references.citation: PLoS One. 2013 May 28;8(5):e65095
references.pmid: 23724124
references.citation: Thyroid. 2009 Oct;19(10):1077-84
references.pmid: 19772429
references.citation: Eur J Endocrinol. 2004 Apr;150(4):547-56
references.pmid: 15080786
references.citation: Thyroid. 2014 Apr;24(4):695-704
references.pmid: 24262022
references.citation: J Clin Endocrinol Metab. 2003 Sep;88(9):4393-7
references.pmid: 12970315
references.citation: Horm Cancer. 2015 Jun;6(2-3):87-99
references.pmid: 25800363
references.citation: J Clin Endocrinol Metab. 2015 Feb;100(2):E232-42
references.pmid: 25423568
references.citation: Methods Enzymol. 2010;479:397-411
references.pmid: 20816179
references.citation: Oncogene. 2014 Nov 20;33(47):5397-404
references.pmid: 24362526