title: Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae).
abstract: Salmonella Enteritidis SE86 is an important foodborne pathogen in Southern Brazil and it is able to produce a biosurfactant. However, the importance of this compound for the microorganism is still unknown. This study aimed to investigate the influence of the biosurfactant produced by S. Enteritidis SE86 on adherence to slices of lettuce leaves and on resistance to sanitizers. First, lettuce leaves were inoculated with S. Enteritidis SE86 in order to determine the amount of biosurfactant produced. Subsequently, lettuce leaves were inoculated with S. Enteritidis SE86 with and without the biosurfactant, and the adherence and bacterial resistance to different sanitization methods were evaluated. S. Enteritidis SE86 produced biosurfactant after 16 h (emulsification index of 11 to 52.15 percent, P < 0.05) and showed greater adherence capability and resistance to sanitization methods when the compound was present. The scanning electron microscopy demonstrated that S. Enteritidis was able to adhere, form lumps, and invade the lettuce leaves' stomata in the presence of the biosurfactant. Results indicated that the biosurfactant produced by S. Enteritidis SE86 contributed to adherence and increased resistance to sanitizers when the microorganism was present on lettuce leaves.
authors.affiliation: Laboratório de Microbiologia, Departamento de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa CatarinaSão Miguel do Oeste, Brazil; Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do SulPorto Alegre, Brazil.
authors.forename: Eliandra M
authors.initials: EM
authors.lastname: Rossi
authors.affiliation: Laboratório de Microbiologia, Departamento de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina São Miguel do Oeste, Brazil.
authors.forename: Luniele
authors.initials: L
authors.lastname: Beilke
authors.affiliation: Laboratório de Microbiologia, Departamento de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina São Miguel do Oeste, Brazil.
authors.forename: Marília
authors.initials: M
authors.lastname: Kochhann
authors.affiliation: Laboratório de Microbiologia, Departamento de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina São Miguel do Oeste, Brazil.
authors.forename: Diana H
authors.initials: DH
authors.lastname: Sarzi
authors.affiliation: Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul Porto Alegre, Brazil.
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Tondo
keywords: Salmonella Enteritidis SE86; biosurfactant; disinfection; lettuce; microbial adherence and resistance
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