title: Nakamurella endophytica sp. nov., a novel endophytic actinobacterium isolated from the bark of Kandelia candel.
abstract: A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain 2Q3S-4-2T, was isolated from the surface-sterilized bark of Kandelia candel, collected from Cotai Ecological Zones in Macao, PR China. It was tested using a polyphasic approach to determine its taxonomic position. Strain 2Q3S-4-2T grew optimally without NaCl at 28-30 °C and at pH 7.0. Substrate mycelia and aerial mycelia were not formed and no diffusible pigments were observed on the media tested. Phylogenetic analysis, based on 16S rRNA gene sequences, suggested that strain 2Q3S-4-2T belonged to the genus Nakamurella, sharing highest 16S rRNA gene sequence similarity with Nakamurella flavida DS-52T (96.76 %). The DNA G+C content of strain 2Q3S-4-2T was 67.8 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid and MK-8(H4) was the predominant menaquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipids and phosphatidylinositol. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0. On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain 2Q3S-4-2T represents a novel species of the genus Nakamurella, for which the name Nakamurella endophytica sp. nov. is proposed. The type strain is 2Q3S-4-2T ( = DSM 100722T = CGMCC 4.7308T).
authors.affiliation: Research Center for Medicine & Biology, Zunyi Medical University, Zunyi 563003, PR China.|Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Tuo
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Fei-Na
authors.initials: FN
authors.lastname: Li
authors.affiliation: Research Center for Medicine & Biology, Zunyi Medical University, Zunyi 563003, PR China.|Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Faculty of Science and Technology, Department of Civil and Environmental Engineering, University of Macau, Macao, PR China.
authors.forename: Inchio
authors.initials: I
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Quality Research of Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Quality Research of Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Simon
authors.initials: S
authors.lastname: Ming-Yuen Lee
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Cheng-Hang
authors.initials: CH
authors.lastname: Sun
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043982:Rhizophoraceae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid