title: Thalassobius litorarius sp. nov., isolated from a tidal flat.
abstract: A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, belonging to the family Rhodobacteraceae, designated strain MME-075T, was isolated from a tidal flat. Strain MME-075T grew with 1-5% (w/v) NaCl at 20-35 °C and at pH 7-9. Optimal growth occurred with 2% (w/v) NaCl at 25-30 °C and at pH 7. The dominant respiratory quinone was ubiquinone-10, whereas summed feature 8 (C18:1ω7c and/or C18:1ω6c), 11-methyl C18:1ω7c, and C16:0 were the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. The genomic DNA G+C content was 62.1 mol%. The highest 16S rRNA gene sequence similarity values were between strain MME-075T and Thalassobius aestuarii, Thalassococcus lentus, Thalassobius maritimus and Shimia marina and were 97.6, 97.5, 97.2 and 96.2%, respectively. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain MME-075T clustered with species of the genus Thalassobius. Based on phenotypic and phylogenetic taxonomic properties, this strain is proposed as a representative of a novel species of the genus Thalassobius, for which the name Thalassobius litorarius sp. nov. is proposed. The type strain is MME-075T (=KCCM 43143T=JCM 30758T).
authors.affiliation: Department of Life Sciences, Graduate School of Incheon National University, Incheon 22012, Republic of Korea.
authors.forename: Inhye
authors.initials: I
authors.lastname: Park
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Cha
authors.affiliation: Department of Life Sciences, Graduate School of Incheon National University, Incheon 22012, Republic of Korea.|Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2