title: Bone Fracture Healing with Umbilico-Placental Mononuclear Cells: A Controlled Animal Study.
abstract: BACKGROUND
  Fracture healing is a significant process in orthopedics. In this controlled animal study, our aimis to expose the healing effects of cord blood umbilico-placental mononuclear cells (UPMNCs) on bone fractures.
  
  
  METHODS
  Caesarean sections were performed on five pregnant New Zealand rabbits at term. Placentas and cords were collected. Standard closed transverse shaft fractures were created on both tibial bones of 15 baby rabbits. The right tibias were given UPMNCs; the left tibias were the control group. Histological examinations, osteoblast and osteoclast cell counts, and mechanical stabilities were compared. Anchorage of the donor cells was shown by the fluorescence in situ hybridization (FISH) technique.
  
  
  RESULTS
  In the group injected with UPMNCs, histopathological fracture healing was faster, osteoblast and osteoclast counts were significantly increased, and the maximum load capacity was higher. The presence of XX and XY chromatins on the same slide revealed the anchorage of female donor cells on male tissues.
  
  
  CONCLUSIONS
  The effects of umbilico-placental mononuclear cells on bone healing are histopathological healing priority, increased osteoblastic and osteoclastic activities (bone turnover), and better mechanical stability.
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey. onurpolat1971@yahoo.com.|Ibni Sina Hospital Emergency, Department Ankara, University Faculty of Medicine Ankara, 06106, Sihhiye, Turkey. onurpolat1971@yahoo.com.
authors.forename: Onur
authors.initials: O
authors.lastname: Polat
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Gurur
authors.initials: G
authors.lastname: Polat
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Sercin
authors.initials: S
authors.lastname: Karahuseyinoglu
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Nüket Yörür
authors.initials: NY
authors.lastname: Kutlay
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Arzu Gül
authors.initials: AG
authors.lastname: Tasci
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Erdemli
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Ajlan
authors.initials: A
authors.lastname: Tukun
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Mustafa Cihat
authors.initials: MC
authors.lastname: Avunduk
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Sükrü
authors.initials: S
authors.lastname: Küplülü
authors.affiliation: Ibni Sina Hospital Emergency Department, Ankara University, Faculty of Medicine Ankara, Sihhiye, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Demirtas
keywords: Bone fracture; Cord blood; Mononuclear; Placenta; Stem cell
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