title: Lingual Epithelial Stem Cells and Organoid Culture of Them.
abstract: As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine.
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 5-1, 2-chome, Shin-machi, Hirakata-shi, Osaka 573-1010, Japan. hishah@hirakata.kmu.ac.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hisha
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 5-1, 2-chome, Shin-machi, Hirakata-shi, Osaka 573-1010, Japan. tanakato@hirakata.kmu.ac.jp.|Third Department of Internal Medicine, Kansai Medical University, 5-1, 2-chome, Shin-machi, Hirakata-shi, Osaka 573-1010, Japan. tanakato@hirakata.kmu.ac.jp.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 5-1, 2-chome, Shin-machi, Hirakata-shi, Osaka 573-1010, Japan. hueno@hirakata.kmu.ac.jp.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D009940:Organoids / Q000166:cytology* / Q000378:metabolism; D055153:Stem Cell Niche; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014059:Tongue / Q000166:cytology* / Q000378:metabolism
keywords: Bmi1; lingual epithelium; organoid; stem cells
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