title: High-Throughput Analysis of Sucrose Fatty Acid Esters by Supercritical Fluid Chromatography/Tandem Mass Spectrometry.
abstract: Supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry was applied to the profiling of sucrose fatty acid esters (SEs). The SFC conditions (column and modifier gradient) were optimized for the effective separation of SEs. In the column test, a silica gel reversed-phase column was selected. Then, the method was used for the detailed characterization of commercial SEs and the successful analysis of SEs containing different fatty acids. The present method allowed for fast and high-resolution separation of monoesters to tetra-esters within a shorter time (15 min) as compared to the conventional high-performance liquid chromatography. The applicability of our method for the analysis of SEs was thus demonstrated.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University; Research and Development Department, Fuji Oil Co., Ltd.
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Hori
authors.affiliation: Research and Development Department, Fuji Oil Co., Ltd.
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Tsumura
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
keywords: emulsifier; sucrose fatty acid esters; supercritical fluid chromatography
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