title: Modulation of the Ca(2+) signaling pathway by celangulin I in the central neurons of Spodoptera exigua.
abstract: Celangulin I is an insecticidal component isolated from Chinese bittersweet Celastrus angulatus. The present study explored the possible effects of celangulin I on the calcium signaling pathway, especially on the L-type Ca(2+) channel and the calcium channels in the endoplasmic reticulum in the central neurons isolated from the third instar larvae of Spodoptera exigua using whole-cell patch-clamp and calcium imaging technique. The results showed that celangulin I could activate the high voltage-gated calcium channel at the concentration of 150μM. The peak currents were increased by 17% of the initial value at the end of the 10-min recording after treated with celangulin I. The rises of intracellular calcium ion concentration ([Ca(2+)]i) in neurons treated by celangulin I showed that the effects of celangulin I were concentration-dependent. Activation of the RyRs by ryanodine decreased the calcium release induced by celangulin I, indicating that celangulin I exerts effect on insect RyRs. Furthermore, we also provided evidence for the first time that celangulin I activates inositol 1,4,5-trisphosphate (IP3) sensitive intracellular calcium release channels in the endoplasmic reticulum third instar larvae neurons of S. exigua. Plausibly, these experimental results can explain the characteristic symptoms of anesthesia and paralysis in celangulin I treated insects.
authors.affiliation: State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China. Electronic address: liyx128@nankai.edu.cn.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Pesticide Science, Key Laboratory of Applied Entomology, Northwest A&F University, Box 61, Xinong Road 22, Yangling, Shanxi 712100, China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Lian
authors.affiliation: State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Yinging
authors.initials: Y
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Duoyi
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Di
authors.affiliation: Institute of Pesticide Science, Key Laboratory of Applied Entomology, Northwest A&F University, Box 61, Xinong Road 22, Yangling, Shanxi 712100, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China. Electronic address: nkzml@vip.163.com.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects*; D007306:Insecticides / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism*; D018411:Spodoptera / Q000378:metabolism*
chemical_list: D007306:Insecticides
keywords: Calcium channel; Celangulin I; Modulate; Neurons; Spodoptera exigua