title: Neuroprotective Effect of Resveratrol on Acute Brain Ischemia Reperfusion Injury by Measuring Annexin V, p53, Bcl-2 Levels in Rats.
abstract: BACKGROUND
  Cerebral ischemia is as a result of insufficient cerebral blood flow for cerebral metabolic functions. Resveratrol is a natural phytoalexin that can be extracted from grape's skin and had potent role in treating the cerebral ischemia. Apoptosis, a genetically programmed cellular event which occurs after ischemia and leads to biochemical and morphological changes in cells. There are some useful markers for apoptosis like Bcl-2, bax, and p53. The last reports, researchers verify the apoptosis with early markers like Annexin V.
  
  
  METHODS
  We preferred in this experimental study a model of global cerebral infarction which was induced by bilateral common carotid artery occlusion method. Rats were randomly divided into 4 groups : sham, ischemia-reperfusion (I/R), I/R plus 20 mg/kg resveratrol and I/R plus 40 mg/kg resveratrol. Statistical analysis was performed using Sigmastat 3.5 ve IBM SPSS Statistics 20. We considered a result significant when p<0.001.
  
  
  RESULTS
  After administration of resveratrol, Bcl-2 and Annexin levels were significantly increased (p<0.001). Depending on the dose of resveratrol, Bcl2 levels increased, p53 levels decreased but Annexin V did not effected. P53 levels were significantly increased in ishemia group, so apoptosis is higher compared to other groups.
  
  
  CONCLUSIONS
  In the acute period, Annexin V levels misleading us because the apoptotic cell counts could not reach a certain level. Therefore we should support our results with bcl-2 and p53.
authors.affiliation: Department of Neurosurgery, Katip Celebi University Izmir Atatürk Training and Research Hospital, Izmir, Turkey.
authors.forename: Ceren
authors.initials: C
authors.lastname: Kizmazoglu
authors.affiliation: Department of Neurosurgery, Eskisehir State Hospital, Eskisehir, Turkey.; Department of Pharmacology, Eskişehir Osmangazi University, Eskisehir, Turkey.
authors.forename: Hasan Emre
authors.initials: HE
authors.lastname: Aydin
authors.affiliation: Department of Neurosurgery, Katip Celebi University Izmir Atatürk Training and Research Hospital, Izmir, Turkey.
authors.forename: Ismail Ertan
authors.initials: IE
authors.lastname: Sevin
authors.affiliation: Department of Neurosurgery, Dokuz Eylul University, School of Medicine Hospital, Izmir, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Kalemci
authors.affiliation: Department of Neurosurgery, Katip Celebi University Izmir Atatürk Training and Research Hospital, Izmir, Turkey.
authors.forename: Nurullah
authors.initials: N
authors.lastname: Yüceer
authors.affiliation: Department of Neurosurgery, Eskişehir Osmangazi University School of Medicine Hospital, Eskisehir, Turkey.
authors.forename: Metin Ant
authors.initials: MA
authors.lastname: Atasoy
keywords: Annexin V; Bcl-2; Brain Ischemia; Resveratrol; p53
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