title: WISP1 mediates hepatic warm ischemia reperfusion injury via TLR4 signaling in mice.
abstract: Wnt-induced secreted protein-1 (WISP1) is an extracellular matrix protein that has been reported in cancer researches. Our previous studies on WISP1 implied it could be a harmful mediator in septic mice. However, its role in liver ischemia reperfusion (I/R) injury is unknown. This study investigated the effects of WISP1 on liver I/R damage. Male C57BL/6 wild-type mice were used to undergo 60 min segmental (70%) ischemia. WISP1 expression was measured after indicated time points of reperfusion. Anti-WISP1 antibody was injected intraperitoneally to mice. Toll-like receptor 4 (TLR4) knockout mice and TIR-domain-containing adaptor inducing interferon-β (TRIF) knockout mice were adopted in this study. WISP1 was significantly enhanced after 6 h of reperfusion when compared with sham treated mice and significantly decreased either by TLR4 knockout mice or TRIF knockout mice. Anti-WISP1 antibody significantly decreased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), pathological changes and pro-inflammatory cytokine levels in the mice following I/R. Furthermore, significantly increased serum transaminase levels were found in C57 wild-type mice treated with recombinant WISP1 protein, but not found in TLR4 knockout or TRIF knockout mice subjected to liver I/R. Taken together, WISP1 might contribute to hepatic ischemia reperfusion injury in mice and possibly depends on TLR4/TRIF signaling.
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Xi-Bing
authors.initials: XB
authors.lastname: Ding
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Zhi-Xia
authors.initials: ZX
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Shu-Qing
authors.initials: SQ
authors.lastname: Jin
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, First Clinical College of Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, School of Medicine, Nanchang University, Nanchang 330031, Jiangxi, China.
authors.forename: Shu-Ya
authors.initials: SY
authors.lastname: Mei
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism; D000818:Animals; D055511:CCN Intercellular Signaling Proteins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D055511:CCN Intercellular Signaling Proteins; D011518:Proto-Oncogene Proteins; D051197:Toll-Like Receptor 4; C492067:CCN4 protein, mouse; C476286:Ticam1 protein, mouse; C493487:Tlr4 protein, mouse
references.citation: J Biol Chem. 2004 Feb 27;279(9):7370-7
references.pmid: 14660645
references.citation: J Immunol. 2004 Jan 1;172(1):20-4
references.pmid: 14688304
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14717-22
references.pmid: 9843955
references.citation: J Immunol. 2004 Dec 15;173(12):7115-9
references.pmid: 15585830
references.citation: J Exp Med. 2005 Apr 4;201(7):1135-43
references.pmid: 15795240
references.citation: J Hepatol. 2006 Sep;45(3):401-9
references.pmid: 16780995
references.citation: J Immunol. 2007 Nov 1;179(9):6318-24
references.pmid: 17947709
references.citation: Hepatology. 2008 Jan;47(1):199-206
references.pmid: 17935177
references.citation: Hepatology. 2008 Jan;47(1):207-14
references.pmid: 18041715
references.citation: Transplantation. 2008 Apr 15;85(7):1016-22
references.pmid: 18408583
references.citation: Hepatology. 2008 Oct;48(4):1224-31
references.pmid: 18792393
references.citation: Int J Biochem Cell Biol. 2009 Apr;41(4):771-83
references.pmid: 18775791
references.citation: J Clin Invest. 2009 Apr;119(4):772-87
references.pmid: 19287097
references.citation: Mediators Inflamm. 2010;2010:704202
references.pmid: 20628516
references.citation: Arthritis Rheum. 2011 Jun;63(6):1527-33
references.pmid: 21360520
references.citation: Am J Transplant. 2011 Aug;11(8):1563-9
references.pmid: 21668640
references.citation: Transplantation. 2012 Sep 15;94(5):532-8
references.pmid: 22885879
references.citation: Am J Respir Cell Mol Biol. 2012 Oct;47(4):528-35
references.pmid: 22700866
references.citation: J Pathol. 2013 Jan;229(2):145-56
references.pmid: 23097158
references.citation: Nat Rev Gastroenterol Hepatol. 2013 Feb;10(2):79-89
references.pmid: 23229329
references.citation: Hepatology. 2013 Apr;57(4):1585-96
references.pmid: 23184590
references.citation: Hepatology. 2013 Jul;58(1):374-87
references.pmid: 23460269
references.citation: Lab Invest. 2013 Jul;93(7):792-800
references.pmid: 23628899
references.citation: Am J Respir Cell Mol Biol. 2013 Jul;49(1):114-27
references.pmid: 23590298
references.citation: J Surg Res. 2013 Oct;184(2):792-3
references.pmid: 22765997
references.citation: Crit Care Med. 2014 Feb;42(2):e123-31
references.pmid: 24231763
references.citation: Nat Rev Rheumatol. 2014 Jun;10(6):320
references.pmid: 24798572
references.citation: J Immunol. 2014 Aug 15;193(4):1787-98
references.pmid: 25024388
references.citation: J Cell Mol Med. 2014 Sep;18(9):1863-73
references.pmid: 24898700
references.citation: J Immunol. 2014 Oct 15;193(8):4223-34
references.pmid: 25210121
references.citation: Cell Death Dis. 2014;5:e1467
references.pmid: 25321474
references.citation: Surgery. 2014 Nov;156(5):1225-31
references.pmid: 25262218
references.citation: Shock. 2015 Apr;43(4):352-60
references.pmid: 25526379
references.citation: Mol Med. 2014;20:639-48
references.pmid: 25375408
references.citation: BMC Cancer. 2015;15:34
references.pmid: 25655081
references.citation: Sci Rep. 2015;5:12940
references.pmid: 26260698
references.citation: J Biol Chem. 2002 Apr 26;277(17):15028-34
references.pmid: 11836257
references.citation: J Biol Chem. 2002 Aug 16;277(33):29399-405
references.pmid: 12050162
references.citation: Science. 2002 Nov 1;298(5595):1025-9
references.pmid: 12411706
references.citation: Annu Rev Immunol. 2003;21:335-76
references.pmid: 12524386
references.citation: J Biol Chem. 2003 Mar 28;278(13):11465-70
references.pmid: 12529380
references.citation: Hepatology. 2003 May;37(5):1043-55
references.pmid: 12717385
references.citation: Hepatology. 1998 Apr;27(4):1172-7
references.pmid: 9537464