title: Influence of cyclopropane fatty acids on heat, high pressure, acid and oxidative resistance in Escherichia coli.
abstract: Heat and high pressure resistant strains of Escherichia coli are a challenge to food safety. This study investigated effects of cyclopropane fatty acids (CFAs) on stress tolerance in the heat- and pressure-resistant strain E. coli AW1.7 and the sensitive strain E. coli MG1655. The role of CFAs was explored by disruption of cfa coding for CFA synthase with an in-frame, unmarked deletion method. Both wild-type strains consumed all the unsaturated fatty acids (C16:1 and C18:1) that were mostly converted to CFAs and a low proportion to saturated fatty acid (C16:0). Moreover, E. coli AW1.7 contained a higher proportion of membrane C19:0 cyclopropane fatty acid than E. coli MG1655 (P<0.05). The Δcfa mutant strains did not produce CFAs, and the corresponding substrates C16:1 and C18:1 accumulated in membrane lipids. The deletion of cfa did not alter resistance to H2O2 but increased the lethality of heat, high pressure and acid treatments in E. coli AW1.7, and E. coli MG1655. E. coli AW1.7 and its Δcfa mutant were more resistant to pressure and heat but less resistant to acid stress than E. coli MG1655. Heat resistance of wild-type strains and their Δcfa mutant was also assessed in beef patties grilled to an internal temperature of 71 °C. After treatment, cell counts of wild type strains were higher than those of the Δcfa mutant strains. In conclusion, CFA synthesis in E. coli increases heat, high pressure and acid resistance, and increases heat resistance in food. This knowledge on mechanisms of stress resistance will facilitate the design of intervention methods for improved pathogen control in food production.
authors.affiliation: University of Alberta, Department of Agricultural, Food and Nutritional Science, Edmonton, Canada.
authors.forename: Yuan Yao
authors.initials: YY
authors.lastname: Chen
authors.affiliation: University of Alberta, Department of Agricultural, Food and Nutritional Science, Edmonton, Canada; Hubei University of Technology, School of Food and Pharmaceutical Engineering, Wuhan, Hubei, PR China. Electronic address: mgaenzle@ualberta.ca.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gänzle
mesh_terms: D000143:Acids / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D003521:Cyclopropanes / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D005227:Fatty Acids / Q000494:pharmacology*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D008460:Meat / Q000382:microbiology; D008563:Membrane Lipids; D011312:Pressure; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D000143:Acids; D003521:Cyclopropanes; D005227:Fatty Acids; D008563:Membrane Lipids; C028775:cyclopropane fatty acids; D006861:Hydrogen Peroxide
keywords: Beef; Cyclopropane fatty acids; Escherichia coli; Heat resistance; Pressure resistance