title: [Toxic effects of mixture of volatile organic compounds on mice testis related enzymes and hormones].
abstract: OBJECTIVE
  To evaluate the toxic effects of mixture of volatile organic compounds (VOCs) on Mice Testis related enzymes and hormones.
  
  
  METHODS
  After determining the median lethal dose (LD₅₀) of VOCs using the acute toxicity test, 40 male clean inbred Kunming mice were assigned to 1/8 LD₅₀ VOCs exposure group, 1/4 LD₅₀ VOCs exposure group, and 1/2 LD₅₀ VOCs exposure group, as well as positive control group with cyclophosphamide (60 mg/kg) and negative control group with tea oil, with 8 mice in each group. The mice were intraperitoneally injected with respective agents for 5 days. The levels of testis testosterone, estradiol, follicle stimulating hormone, and luteinizing hormone were determined by ELISA. Meanwhile, the activity of testicular marked enzymes such as lactate dehydrogenase, gamma-glutamyl transpeptidase, acid phosphatase, and glucose-6-phosphate dehydrogenase were examined.
  
  
  RESULTS
  Compared with the negative control group, the 1/8 LD₅₀ exposure group had a significantly increased testis coefficient (P<0.05). Both the activity of testicular marked enzymes and the levels of testicular sex hormones in all exposure groups showed significant downward trends with increasing VOC doses compared with those in the negative control group (P<0.05).
  
  
  CONCLUSIONS
  VOCs have obvious toxicity to mouse testis by changing the levels of testicular sex hormones and the activity of testicular marked enzymes.
authors.affiliation: Hangzhou Center for Disease Control and Prevention, Hangzhou 310006, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hao
authors.forename: Jinjiang
authors.initials: J
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Guo
authors.forename: Nana
authors.initials: N
authors.lastname: Qi
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Yang
authors.forename: Xuhui
authors.initials: X
authors.lastname: Zhang
authors.forename: Luoxian
authors.initials: L
authors.lastname: Yang
authors.affiliation: E-mail: zhangzengli@suda.edu.cn.
authors.forename: Zengli
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004958:Estradiol / Q000737:chemistry; D005640:Follicle Stimulating Hormone / Q000737:chemistry; D012739:Gonadal Steroid Hormones / Q000737:chemistry*; D007986:Luteinizing Hormone / Q000737:chemistry; D008297:Male; D051379:Mice; D013737:Testis / Q000737:chemistry / Q000187:drug effects*; D013739:Testosterone / Q000737:chemistry; D055549:Volatile Organic Compounds / Q000633:toxicity*
chemical_list: D012739:Gonadal Steroid Hormones; D055549:Volatile Organic Compounds; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone