title: Brachypodium distachyon is a suitable host plant for study of Barley yellow dwarf virus.
abstract: Barley yellow dwarf viruses (BYDVs) belong to the family Luteoviridae and cause disease in cereals. Because of the large and complex genome of cereal plants, it is difficult to study host-virus interactions. In order to establish a model host system for the studies on BYDVs, we examined the susceptibility of a monocot model plant, Brachypodium distachyon, to BYDV-GAV infection. Fourteen days after BYDV-GAV inoculation by aphid transmission, B. distachyon plants (inbred line Bd21-3) showed conspicuous disease symptoms such as leaf reddening, dwarfness and root stunting. Virus accumulation was detected in both shoots and roots using reverse transcription PCR and triple antibody sandwich ELISA. Compared with infected wheat plants, B. distachyon plants developed more severe disease symptoms and accumulated a higher level of BYDV-GAV. Under transmission electron microscope, we observed that virus particles accumulated in companion cells and BYDV-GAV infection was associated with the deformation of chloroplasts in the infected leaves of B. distachyon plants. Our results suggest that B. distachyon is a suitable and promising experimental model plant for the host-BYDV-GAV pathosystem and possibly for other BYDVs.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Soumou Wansim
authors.initials: SW
authors.lastname: Nadege
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China. wuyf@nwsuaf.edu.cn.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wu
mesh_terms: D058431:Brachypodium / Q000821:virology*; D054884:Host-Pathogen Interactions; D017793:Luteovirus / Q000502:physiology*; D010935:Plant Diseases / Q000821:virology; D014908:Triticum / Q000821:virology; D056189:Viral Tropism
keywords: Barley yellow dwarf virus; Brachypodium distachyon; Model plant; Virus disease; Virus-host pathosystem
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