title: A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration.
abstract: Cell migration is a critical determinant of cancer invasion and metastasis. Drugs targeting cancer cell migration have been hindered due to the lack of effective assays for monitoring cancer cell migration. Here we describe a novel method to microscopically monitor cell migration in a quantitative fashion. This assay can be used to study genes involved in cancer cell migration, as well as screening anticancer drugs that target this cellular process.
authors.affiliation: Division of Cancer Prevention, Department of Medicine, Stony Brook University, 101 Nicolls Road, Stony Brook, NY, 11794, USA.
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Alford
authors.affiliation: Division of Cancer Prevention, Department of Medicine, Stony Brook University, 101 Nicolls Road, Stony Brook, NY, 11794, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Roth
authors.affiliation: Division of Cancer Prevention, Department of Medicine, Stony Brook University, 101 Nicolls Road, Stony Brook, NY, 11794, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Division of Cancer Prevention, Department of Medicine, Stony Brook University, 101 Nicolls Road, Stony Brook, NY, 11794, USA. jian.cao@stonybrook.edu.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
mesh_terms: D045744:Cell Line, Tumor; D054443:Cell Migration Assays / Q000379:methods*; D024042:Collagen Type I / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans
chemical_list: D024042:Collagen Type I
keywords: Microscope; Migration; Non-tissue culture 96-well plate; Two-dimensional culture; Type I collagen