title: Elevated Serum Brain-Derived Neurotrophic Factor (BDNF) but not BDNF Gene Val66Met Polymorphism Is Associated with Autism Spectrum Disorders.
abstract: The aim of our study was to illuminate the potential role of brain-derived neurotrophic factor (BDNF) in autism spectrum disorder (ASD). We measured the circulating levels of BDNF in serum and BDNF gene (Val66Met) polymorphisms, in which two indicators were then compared between ASD and normal controls. A total of 82 drug-naïve ASD children and 82 age- and gender-matched normal controls were enrolled in the study. Their serum BDNF levels were detected by the ELISA. BDNF Val66Met polymorphism genotyping was conducted as according to the laboratory's standard protocol in laboratory. The ASD severity assessment was mainly determined by the score of the Childhood Autism Rating Scale (CARS). ELISA assay showed that the mean serum BDNF level of children with ASD was significantly (P < 0.0001) higher than that of the control cases (17.75 ± 5.43 vs. 11.49 ± 2.85 ng/ml; t = 9.236). Besides, the serum BDNF levels and CARS scores (P < 0.0001) were positively related. And, the BDNF genotyping results showed that there was no difference between the ASD cases and the control. Among the children with ASD, the mean serum BDNF level of Met/Met group was lower than other groups. According to the ROC curve generated from our clinical data, the optimal cutoff value of serum BDNF levels, an indicator for diagnosis of ASD, was projected to be 12.50 ng/ml. Thus, it yielded a corresponding sensitivity of 81.7 % and the specificity of 66.9 %. Accordingly, area value under the curve was 0.836 (95 % CI, 0.774-0.897); the positive predictive value (PPV) and the negative predictive value (NPV) were 70.1 and 79.1 %, respectively. These results suggested that rather than Val66Met polymorphism, BDNF was more possible to impact the pathogenesis of ASD.
authors.affiliation: Department of Laboratory Medicine, Liaocheng People's Hospital, No. 67, Dongchang West Road, Liaocheng, 252000, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Meng
authors.affiliation: Department of Laboratory Medicine, Liaocheng People's Hospital, No. 67, Dongchang West Road, Liaocheng, 252000, China. wangyws72@163.com.
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Liaocheng Herbalist Hospital, Liaocheng, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Liaocheng People's Hospital, No. 67, Dongchang West Road, Liaocheng, 252000, China.
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Chu
authors.affiliation: Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, No. 238, Baiti Road, Tianjin, 300192, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Tu
authors.affiliation: Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, No. 238, Baiti Road, Tianjin, 300192, China. liuqiang@irm-cams.ac.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000067877:Autism Spectrum Disorder / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D056726:Genetic Association Studies / Q000379:methods*; D006801:Humans; D008297:Male; D008715:Methionine / Q000235:genetics; D011110:Polymorphism, Genetic / Q000502:physiology*; D014633:Valine / Q000235:genetics
chemical_list: D015415:Biomarkers; D019208:Brain-Derived Neurotrophic Factor; C579455:BDNF protein, human; D008715:Methionine; D014633:Valine
keywords: Autism spectrum disorders; BDNF Val66Met polymorphism; Biomarker; Brain-derived neurotrophic factor; Chinese
references.citation: Neurosci Biobehav Rev. 2015 Apr;51:15-30
references.pmid: 25562189
references.citation: Mol Psychiatry. 2009 Feb;14(2):120-2
references.pmid: 19156154
references.citation: J Autism Dev Disord. 1980 Mar;10(1):91-103
references.pmid: 6927682
references.citation: Cell. 2003 Jan 24;112(2):257-69
references.pmid: 12553913
references.citation: J Coll Physicians Surg Pak. 2014 Apr;24(4):274-8
references.pmid: 24709243
references.citation: J Psychiatr Res. 2015 Feb;61:81-8
references.pmid: 25498133
references.citation: Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):313
references.pmid: 25736402
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2006 Dec 30;30(8):1529-31
references.pmid: 16876305
references.citation: Int J Neuropsychopharmacol. 2011 Apr;14(3):347-53
references.pmid: 20604989
references.citation: Int J Dev Neurosci. 2006 Feb;24(1):73-80
references.pmid: 16289943
references.citation: Clin Chem Lab Med. 2013 Feb;51(2):e19-22
references.pmid: 23095201
references.citation: Neuroreport. 2015 Apr 15;26(6):341-5
references.pmid: 25714424
references.citation: J Child Neurol. 2008 Jan;23(1):67-72
references.pmid: 18160559
references.citation: Int J Dev Neurosci. 2007 Oct;25(6):367-72
references.pmid: 17804189
references.citation: Eur J Paediatr Neurol. 2015 Jul;19(4):411-4
references.pmid: 25847750
references.citation: Int J Dev Neurosci. 2014 Oct;37:65-8
references.pmid: 24984148
references.citation: J Physiol. 2006 Aug 1;574(Pt 3):787-803
references.pmid: 16709633
references.citation: Neurotox Res. 2013 Nov;24(4):491-501
references.pmid: 23604965
references.citation: Yonsei Med J. 2010 Sep;51(5):661-71
references.pmid: 20635439
references.citation: J Autism Dev Disord. 2009 Nov;39(11):1542-51
references.pmid: 19582565
references.citation: Neuropsychobiology. 2012 Jun;65(4):183-7
references.pmid: 22538247
references.citation: J Neurodev Disord. 2009 Sep;1(3):215-23
references.pmid: 21547716
references.citation: J Neurosci. 2003 Jul 30;23(17):6690-4
references.pmid: 12890761
references.citation: Science. 2006 Oct 6;314(5796):140-3
references.pmid: 17023662
references.citation: J Neural Transm (Vienna). 2014 Sep;121(9):1117-28
references.pmid: 24500031
references.citation: Psychiatry Investig. 2014 Jul;11(3):319-24
references.pmid: 25110506
references.citation: Arch Gen Psychiatry. 2007 Apr;64(4):410-6
references.pmid: 17404118
references.citation: J Biomed Nanotechnol. 2009 Oct;5(5):542-50
references.pmid: 20201430
references.citation: Mol Neurobiol. 2016 Oct;53(8):5492-9
references.pmid: 26454822
references.citation: Neurochem Res. 2012 Feb;37(2):394-400
references.pmid: 21984201
references.citation: Brain Res Brain Res Rev. 2002 Jun;39(1):29-54
references.pmid: 12086707
references.citation: Biochem Biophys Res Commun. 2007 Apr 27;356(1):200-6
references.pmid: 17349978
references.citation: Neurobiol Aging. 2006 Dec;27(12):1834-7
references.pmid: 16343697