title: Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species.
abstract: BACKGROUND
  The uncultivated species Dasypyrum breviaristatum carries novel diseases resistance and agronomically important genes of potential use for wheat improvement. The development of new wheat-D. breviaristatum derivatives lines with disease resistance provides an opportunity for the identification and localization of resistance genes on specific Dasypyrum chromosomes. The comparison of wheat-D. breviaristatum derivatives to the wheat-D. villosum derivatives enables to reveal the genomic divergence between D. breviaristatum and D. villosum.
  
  
  RESULTS
  The mitotic metaphase of the wheat- D. breviaristatum partial amphiploid TDH-2 and durum wheat -D. villosum amphiploid TDV-1 were studied using multicolor fluorescent in situ hybridization (FISH). We found that the distribution of FISH signals of telomeric, subtelomeric and centromeric regions on the D. breviaristatum chromosomes was different from those of D. villosum chromosomes by the probes of Oligo-pSc119.2, Oligo-pTa535, Oligo-(GAA)7 and Oligo-pHv62-1. A wheat line D2139, selected from a cross between wheat lines MY11 and TDH-2, was characterized by FISH and PCR-based molecular markers. FISH analysis demonstrated that D2139 contained 44 chromosomes including a pair of D. breviaristatum chromosomes which had originated from the partial amphiploid TDH-2. Molecular markers confirmed that the introduced D. breviaristatum chromosomes belonged to homoeologous group 7, indicating that D2139 was a 7V(b) disomic addition line. The D2139 displayed high resistance to wheat stripe rust races at adult stage plant, which may be inherited from, D. breviaristatum chromosome 7V(b).
  
  
  CONCLUSIONS
  The study present here revealed that the large divergence between D. breviaristatum and D. villosum with respected to the organization of different repetitive sequences. The identified wheat- D. breviaristatum chromosome addition line D2139 will be used to produce agronomically desirable germplasm for wheat breeding.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Dan
authors.initials: D
authors.lastname: Gao
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Hongjin
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Shixiao
authors.initials: S
authors.lastname: La
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Ma
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.
authors.forename: Zujun
authors.identifier: 0000-0003-4617-1422
authors.initials: Z
authors.lastname: Yang
keywords: Dasypyrum breviaristatum; Fluorescence in situ hybridization; Molecular marker; Wheat
references.citation: Chromosoma. 2012 Dec;121(6):597-611
references.pmid: 23052335
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3238-43
references.pmid: 16492777
references.citation: Theor Appl Genet. 1995 Nov;91(6-7):1125-8
references.pmid: 24170007
references.citation: Cytogenet Genome Res. 2014;143(4):280-7
references.pmid: 25247402
references.citation: Genetics. 2011 Apr;187(4):1011-21
references.pmid: 21242535
references.citation: Chromosoma. 1997 Jun;106(1):53-61
references.pmid: 9169587
references.citation: J Appl Genet. 2014 Aug;55(3):313-8
references.pmid: 24782110
references.citation: J Genet. 2012;91(3):343-8
references.pmid: 23271019
references.citation: Genome. 2011 Oct;54(10):836-44
references.pmid: 21961939
references.citation: Theor Appl Genet. 2006 Dec;114(1):41-7
references.pmid: 17016687
references.citation: Hereditas. 2005 Feb;142(2005):80-5
references.pmid: 16970616
references.citation: Chromosoma. 2016 Mar;125(1):163-72
references.pmid: 26238987
references.citation: Theor Appl Genet. 2010 Aug;121(3):489-97
references.pmid: 20383487
references.citation: Mol Genet Genomics. 2014 Aug;289(4):553-65
references.pmid: 24609469
references.citation: PLoS One. 2013 Jul 30;8(7):e70483
references.pmid: 23936213
references.citation: Cytogenet Genome Res. 2015;145(1):68-74
references.pmid: 25968454
references.citation: Theor Appl Genet. 1995 Mar;90(3-4):526-33
references.pmid: 24173947
references.citation: Hereditas. 2006 Dec;143(2006):47-54
references.pmid: 17362333
references.citation: Sci Rep. 2015 May 21;5:10552
references.pmid: 25994088
references.citation: Theor Appl Genet. 1987 Jan;73(3):337-42
references.pmid: 24240992
references.citation: Breed Sci. 2011 Dec;61(4):366-72
references.pmid: 23136473
references.citation: Chromosome Res. 1999;7(3):205-12
references.pmid: 10421380
references.citation: Theor Appl Genet. 2009 Feb;118(3):499-514
references.pmid: 19057889
references.citation: Theor Appl Genet. 2011 Jun;123(1):159-67
references.pmid: 21437597