title: Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster.
abstract: Selenium (Se) is an important trace element for many organisms and is incorporated into selenoproteins as selenocysteine (Sec). In eukaryotes, selenophosphate synthetase SPS2 is essential for Sec biosynthesis. In recent years, genetic disruptions of both Sec biosynthesis genes and selenoprotein genes have been investigated in different animal models, which provide important clues for understanding the Se metabolism and function in these organisms. However, a systematic study on the knockdown of SPS2 has not been performed in vivo. Herein, we conducted microarray experiments to study the transcriptome of fruit flies with knockdown of SPS2 in larval and adult stages. Several hundred differentially expressed genes were identified in each stage. In spite that the expression levels of other Sec biosynthesis genes and selenoprotein genes were not significantly changed, it is possible that selenoprotein translation might be reduced without impacting the mRNA level. Functional enrichment and network-based analyses revealed that although different sets of differentially expressed genes were obtained in each stage, they were both significantly enriched in the carbohydrate metabolism and redox processes. Furthermore, protein-protein interaction (PPI)-based network clustering analysis implied that several hub genes detected in the top modules, such as Nimrod C1 and regucalcin, could be considered as key regulators that are responsible for the complex responses caused by SPS2 knockdown. Overall, our data provide new insights into the relationship between Se utilization and several fundamental cellular processes as well as diseases.
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. lnchen@sibs.ac.cn and Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. yanzhang01@sibs.ac.cn hysong@sibs.ac.cn and Key Laboratory of Food Safety Risk Assessment Ministry of Health, Beijing, China.
authors.forename: Gaopeng
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. yanzhang01@sibs.ac.cn hysong@sibs.ac.cn and Key Laboratory of Food Safety Risk Assessment Ministry of Health, Beijing, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. yanzhang01@sibs.ac.cn hysong@sibs.ac.cn and Key Laboratory of Food Safety Risk Assessment Ministry of Health, Beijing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. lnchen@sibs.ac.cn.
authors.forename: Luonan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. yanzhang01@sibs.ac.cn hysong@sibs.ac.cn and Key Laboratory of Food Safety Risk Assessment Ministry of Health, Beijing, China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Song
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. yanzhang01@sibs.ac.cn hysong@sibs.ac.cn and Key Laboratory of Food Safety Risk Assessment Ministry of Health, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D017344:Genes, Insect; D007814:Larva / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D060066:Protein Interaction Maps / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D029721:Drosophila Proteins; D010770:Phosphotransferases; C499550:Sps2 protein, Drosophila