title: Tumour Necrosis Factor-alpha (TNF-α) and its soluble receptor type 1 (sTNFR I) in human active and healed leishmaniases.
abstract: The role of tumour necrosis factor-alpha (TNF-α) is not fully understood in human leishmaniasis. We analysed the alterations in the levels of TNF-α, soluble TNF receptor type 1 (sTNFR I), IL-17 and IL-22 productions in active and healed leishmaniases. Blood samples were collected from volunteers with active cutaneous leishmaniasis (ACL), the same subjects after lesion healing (healed CL = HCL), volunteers with active visceral leishmaniasis (AVL), healed VL (HVL) and healthy controls. Levels of cytokines were titrated on Leishmania Ag-stimulated PBMC culture. The mean level of TNF-α production from stimulated cells was significantly higher in ACL than controls (P < 0·001) and significantly reduced after treatment in HCL volunteers (P < 0·05). The mean level of sTNFR I production was significantly higher in ACL than controls (P < 0·001) and significantly reduced after treatment in HCL volunteers (P < 0·05). The mean level of IL-22 production in AVL was significantly higher than controls (P < 0·05) and was significantly lower in HVL compared with AVL (P < 0·001) and controls (P < 0·05). The levels of TNF-α (P = 0·0025) and sTNFR I (P < 0·01) productions from PBMCs showed significant decreasing trend after treatment in each CL volunteer. Reduction in TNF-α is associated with clinical response to treatment and healing of CL lesions due to L. major.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.|Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Nateghi Rostami
authors.affiliation: Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Seyyedan Jasbi
authors.affiliation: Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Khamesipour
authors.affiliation: Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: A M
authors.initials: AM
authors.lastname: Mohammadi
mesh_terms: D000328:Adult; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood; D007378:Interleukins / Q000097:blood; D016773:Leishmaniasis, Cutaneous / Q000097:blood* / Q000188:drug therapy; D007898:Leishmaniasis, Visceral / Q000097:blood* / Q000188:drug therapy; D008297:Male; D008536:Meglumine / Q000627:therapeutic use; D000077485:Meglumine Antimoniate; D008875:Middle Aged; D009942:Organometallic Compounds / Q000627:therapeutic use; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood*; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D000981:Antiprotozoal Agents; D020381:Interleukin-17; D007378:Interleukins; D008536:Meglumine; D000077485:Meglumine Antimoniate; D009942:Organometallic Compounds; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22; C439524:TNF protein, human; C506235:TNFRSF1A protein, human
keywords: IL-17; IL22; TNF-α; cutaneous leishmaniasis; sTNFR I