title: Recent development of temperature-responsive surfaces and their application for cell sheet engineering.
abstract: Cell sheet engineering, which fabricates sheet-like tissues without biodegradable scaffolds, has been proposed as a novel approach for tissue engineering. Cells have been cultured and proliferate to confluence on a temperature-responsive cell culture surface at 37°C. By decreasing temperature to 20°C, an intact cell sheet can be harvested from the culture surface without enzymatic treatment. This new approach enables cells to keep their cell-cell junction, cell surface proteins and extracellular matrix. Therefore, recovered cell sheet can be easily not only transplanted to host tissue, but also constructed a three-dimensional (3D) tissue by layering cell sheets. Moreover, cell sheet manipulation technology and bioreactor have been combined with the cell sheet technology to fabricate a complex and functional 3D tissue in vitro. So far, cell sheet technology has been applied in regenerative medicine for several tissues, and a number of clinical studies have been performed. In this review, recent advances in the preparation of temperature-responsive cell culture surface, the fabrication of organ-like tissue and the clinical application of cell sheet engineering are summarized and discussed.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Zhonglan
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
keywords: cell sheet; cell sheet engineering; poly(N-isoproplyacrylamide); temperature-responsive cell culture surface
references.citation: Adv Mater. 2010 Dec 21;22(48):5443-62
references.pmid: 20842659
references.citation: Nat Protoc. 2012 Apr 05;7(5):850-8
references.pmid: 22481530
references.citation: Br Med Bull. 2011;98:143-59
references.pmid: 21596713
references.citation: Hepatol Res. 2008;38(11):1140-7
references.pmid: 18513332
references.citation: Tissue Eng Part A. 2013 Sep;19(17-18):2045-62
references.pmid: 23638920
references.citation: J Biomed Mater Res. 2000 Apr;50(1):82-9
references.pmid: 10644967
references.citation: Transplant Proc. 2011 Nov;43(9):3188-91
references.pmid: 22099753
references.citation: Lancet. 2005 Jun 18-24;365(9477):2122-4
references.pmid: 15964449
references.citation: Nat Commun. 2013;4:1399
references.pmid: 23360990
references.citation: Ann Thorac Cardiovasc Surg. 2009 Dec;15(6):362-7
references.pmid: 20081743
references.citation: Biomaterials. 2009 Oct;30(30):5943-9
references.pmid: 19674781
references.citation: J Biol Chem. 1991 Jul 15;266(20):12809-12
references.pmid: 2071570
references.citation: Exp Cell Res. 1974 Mar 15;84(1):207-18
references.pmid: 4361714
references.citation: Science. 1993 May 14;260(5110):920-6
references.pmid: 8493529
references.citation: Anal Chem. 2008 Feb 15;80(4):1323-7
references.pmid: 18211096
references.citation: Plast Reconstr Surg. 2010 Mar;125(3):901-9
references.pmid: 20009787
references.citation: J Biomater Sci Polym Ed. 2014;25(5):431-43
references.pmid: 24328952
references.citation: Circulation. 2005 May 3;111(17):2198-202
references.pmid: 15851598
references.citation: Biomaterials. 2007 Nov;28(33):4939-46
references.pmid: 17709135
references.citation: Clin Orthop Relat Res. 2005 Jun;(435):96-105
references.pmid: 15930926
references.citation: Langmuir. 2004 Jun 22;20(13):5506-11
references.pmid: 15986693
references.citation: Regen Med. 2012 Sep;7(5):697-712
references.pmid: 22954440
references.citation: Arch Pharm Res. 2014 Jan;37(1):96-106
references.pmid: 24293063
references.citation: Langmuir. 2008 Dec 2;24(23):13632-9
references.pmid: 18980353
references.citation: Gastrointest Endosc. 2012 Oct;76(4):873-81
references.pmid: 22867446
references.citation: Macromol Biosci. 2012 Jun;12(6):751-60
references.pmid: 22517674
references.citation: Biomaterials. 1999 Jul;20(14):1339-44
references.pmid: 10403052
references.citation: Circ Res. 2002 Feb 22;90(3):e40
references.pmid: 11861428
references.citation: Biomaterials. 2004 May;25(11):2047-53
references.pmid: 14741619
references.citation: J Tissue Eng Regen Med. 2015 Apr;9(4):343-56
references.pmid: 23881816
references.citation: Biomaterials. 2012 Oct;33(30):7405-11
references.pmid: 22818649
references.citation: Biomacromolecules. 2004 Mar-Apr;5(2):505-10
references.pmid: 15003013
references.citation: Langmuir. 2005 Mar 1;21(5):1949-55
references.pmid: 15723494
references.citation: Int J Cancer. 1969 Mar 15;4(2):159-65
references.pmid: 4186775
references.citation: FASEB J. 2004 Jul;18(10):1153-5
references.pmid: 15155562
references.citation: Biomacromolecules. 2000 Fall;1(3):377-81
references.pmid: 11710126
references.citation: Biomaterials. 2012 May;33(15):3846-51
references.pmid: 22369960
references.citation: Biomaterials. 2010 Oct;31(29):7435-43
references.pmid: 20647153
references.citation: J Biomed Mater Res A. 2014 Nov;102(11):3883-93
references.pmid: 24339415
references.citation: J Tissue Eng Regen Med. 2016 Mar;10 (3):E188-94
references.pmid: 23894137
references.citation: J Tissue Eng Regen Med. 2013 Feb;7(2):85-98
references.pmid: 22072398
references.citation: Annu Rev Biomed Eng. 2011 Aug 15;13:27-53
references.pmid: 21417722
references.citation: Tissue Eng. 2001 Apr;7(2):141-51
references.pmid: 11304450
references.citation: Biotechnol Bioeng. 1997 Feb 5;53(3):339-44
references.pmid: 18633989
references.citation: Tissue Eng Part C Methods. 2011 Jun;17(6):677-86
references.pmid: 21375407
references.citation: Biomaterials. 2012 Feb;33(5):1406-13
references.pmid: 22118777
references.citation: Biomaterials. 2014 Mar;35(8):2428-35
references.pmid: 24370007
references.citation: Biomaterials. 2006 Sep;27(27):4765-74
references.pmid: 16737736
references.citation: Adv Mater. 2012 Apr 10;24(14):1782-804
references.pmid: 22410857
references.citation: Biomaterials. 2007 Dec;28(36):5471-6
references.pmid: 17869335
references.citation: J Biomater Sci Polym Ed. 2000;11(1):101-10
references.pmid: 10680611
references.citation: J Tissue Eng Regen Med. 2016 Jun;10(6):486-95
references.pmid: 23868865
references.citation: Curr Stem Cell Res Ther. 2012 May;7(3):179-90
references.pmid: 22023626
references.citation: Eur Cell Mater. 2005 Aug 08;10:23-30; discussion 23-30
references.pmid: 16088852
references.citation: Biomaterials. 2005 Nov;26(33):6415-22
references.pmid: 16011847
references.citation: Biomaterials. 2005 May;26(14):1885-93
references.pmid: 15576162
references.citation: Biochim Biophys Acta. 2011 Mar;1810(3):239-50
references.pmid: 20655984
references.citation: Biomaterials. 2000 Dec;21(24):2529-43
references.pmid: 11071603
references.citation: Regen Med. 2010 Sep;5(5):763-75
references.pmid: 20868331
references.citation: Trends Biotechnol. 2012 Oct;30(10):546-54
references.pmid: 22939815
references.citation: Trans Am Ophthalmol Soc. 1999;97:891-986
references.pmid: 10703147
references.citation: Tissue Eng Part B Rev. 2008 Mar;14(1):105-18
references.pmid: 18454637
references.citation: Biomaterials. 2003 Mar;24(7):1223-32
references.pmid: 12527263
references.citation: Tissue Eng. 2003 Aug;9(4):567-77
references.pmid: 13678436
references.citation: J Biomed Mater Res. 2001 Jan;54(1):37-46
references.pmid: 11077401
references.citation: J Biomed Mater Res A. 2005 Oct 1;75(1):1-13
references.pmid: 16086418
references.citation: J Orthop Res. 2008 Sep;26(9):1263-8
references.pmid: 18404654
references.citation: Tissue Eng. 2005 Mar-Apr;11(3-4):469-78
references.pmid: 15869425
references.citation: Eur J Cardiothorac Surg. 2001 Apr;19(4):424-30
references.pmid: 11306307
references.citation: Transplantation. 2004 Feb 15;77(3):379-85
references.pmid: 14966411
references.citation: J Biomed Mater Res A. 2004 Aug 1;70(2):159-68
references.pmid: 15227660
references.citation: Nat Med. 2007 Jul;13(7):880-5
references.pmid: 17572687
references.citation: Tissue Eng Part A. 2009 Jun;15(6):1257-68
references.pmid: 18928400
references.citation: Biomaterials. 2005 Dec;26(36):7616-27
references.pmid: 16005510
references.citation: FASEB J. 2006 Apr;20(6):708-10
references.pmid: 16439619
references.citation: Langmuir. 2005 Aug 16;21(17):7833-41
references.pmid: 16089389
references.citation: Biomaterials. 2013 Jun;34(17):4214-22
references.pmid: 23498894
references.citation: Gastroenterology. 2012 Sep;143(3):582-8.e1-2
references.pmid: 22561054
references.citation: J Biomed Mater Res. 1999 Jan;44(1):44-52
references.pmid: 10397903
references.citation: J Biomed Mater Res. 1999 Jun 15;45(4):355-62
references.pmid: 10321708
references.citation: Tissue Eng. 2001 Aug;7(4):473-80
references.pmid: 11506735
references.citation: Biomacromolecules. 2010 Aug 9;11(8):1991-9
references.pmid: 20593758
references.citation: J Periodontal Res. 2005 Jun;40(3):245-51
references.pmid: 15853971
references.citation: Acta Biomater. 2009 Jan;5(1):470-6
references.pmid: 18676189
references.citation: Transplant Proc. 2013 Jun;45(5):1881-4
references.pmid: 23769062
references.citation: Eur J Cardiothorac Surg. 2000 May;17(5):587-91
references.pmid: 10814924
references.citation: Biotechnol Prog. 2010 Nov-Dec;26(6):1705-13
references.pmid: 20574992
references.citation: Acta Biomater. 2005 Sep;1(5):575-82
references.pmid: 16701837
references.citation: J Biomed Mater Res. 1993 Oct;27(10):1243-51
references.pmid: 8245039
references.citation: Biomacromolecules. 2011 May 9;12(5):1414-8
references.pmid: 21384842
references.citation: Tissue Eng Part A. 2009 Dec;15(12):3943-9
references.pmid: 19566441
references.citation: In Vitro. 1974 Jul-Aug;10:97-111
references.pmid: 4143484
references.citation: Biomacromolecules. 2010 Oct 11;11(10):2583-92
references.pmid: 20836521
references.citation: Cell Stem Cell. 2013 Mar 7;12(3):275-84
references.pmid: 23472869
references.citation: Invest Ophthalmol Vis Sci. 2012 Mar 13;53(3):1325-31
references.pmid: 22064987
references.citation: Biomaterials. 2008 May;29(13):2073-81
references.pmid: 18261791
references.citation: Surg Today. 2012 Jan;42(2):181-4
references.pmid: 22200756
references.citation: N Engl J Med. 2004 Sep 16;351(12):1187-96
references.pmid: 15371576
references.citation: Macromol Biosci. 2011 Mar 10;11(3):400-9
references.pmid: 21104702
references.citation: Gut. 2006 Dec;55(12):1704-10
references.pmid: 16709659
references.citation: Macromol Biosci. 2009 Nov 10;9(11):1069-79
references.pmid: 19530127
references.citation: Sci Rep. 2013;3:1316
references.pmid: 23419835