title: Biological Processes Discovered by High-Throughput Sequencing.
abstract: Advances in DNA and RNA sequencing technologies have completely transformed the field of genomics. High-throughput sequencing (HTS) is now a widely used and accessible technology that allows scientists to sequence an entire transcriptome or genome in a timely and cost-effective manner. Application of HTS techniques has led to many key discoveries, including the identification of long noncoding RNAs, microDNAs, a family of small extrachromosomal circular DNA species, and tRNA-derived fragments, which are a group of small non-miRNAs that are derived from tRNAs. Furthermore, public sequencing repositories provide unique opportunities for laboratories to parse large sequencing databases to identify proteins and noncoding RNAs at a scale that was not possible a decade ago. Herein, we review how HTS has led to the discovery of novel nucleic acid species and uncovered new biological processes during the course.
authors.affiliation: Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Reon
authors.affiliation: Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia; Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia. Electronic address: ad8q@virginia.edu.
authors.forename: Anindya
authors.initials: A
authors.lastname: Dutta
mesh_terms: D000818:Animals; D001686:Biological Phenomena / Q000235:genetics*; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods; D017423:Sequence Analysis, RNA / Q000379:methods; D059467:Transcriptome / Q000235:genetics*
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