title: Release of celecoxib from a bi-layer biomimetic tendon sheath to prevent tissue adhesion.
abstract: Posttraumatic tendon adhesion limits the motion of the limbs greatly. Biomimetic tendon sheaths have been developed to promote tendon healing and gliding. However, after introduction of these biomaterials, the associated inflammatory responses can decrease the anti-adhesion effect. Celecoxib is a non-steroidal anti-inflammatory drug (NSAID) that can decrease inflammation responses. We blended hyaluronic acid and poly(l-lactic acid)-polyethylene glycol (PELA) with microgel electrospinning technology to form an inner layer of a bi-layer biomimetic sheath using sequential electrospinning of an outer celecoxib-PELA layer. Electrospun bi-layer fibrous membranes were mechanically tested and characterized by morphology, surface wettability, and drug release. The tensile strength showed a decreased trend and water contact angles were 114.7 ± 3.9°, 103.6 ± 4.4°, 116.3 ± 5.1°, 122.8 ± 4.7°, and 126.5 ± 4.2° for the surface of PELA, hyaluronic acid-PELA, 2, 6, and 10% celecoxib-PELA electrospun fibrous membranes, respectively. In vitro drug release studies confirmed burst release and then sustained release from the fibrous membranes containing celecoxib for 20 days. In a chicken model of flexor digitorum profundus tendon surgery, the outer celecoxib/PELA layer offered advanced anti-adhesion roles compared to the outer PELA layer and the inner hyaluronic acid-loaded PELA layer still offered tendon healing and gliding. Thus, celecoxib-loaded anti-adhesive tendon sheaths can continuously offer bi-layer biomimetic tendon sheath effects with celecoxib release from the outer layer to prevent tendon adhesion.
authors.affiliation: Department of Orthopaedics, The Third Hospital of Jinan, No. 1 Industrial Road, Wangsheren North Street, Jinan City, Shandong 250132, PR China.
authors.forename: Laifeng
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, PR China.
authors.forename: Xianyou
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, PR China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, PR China.
authors.forename: Shiyang
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Anesthesia, Shanghai Fire Corps Hospital, 26 Sanquan Road, Shanghai 200233, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, PR China.
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
authors.affiliation: Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China. Electronic address: wgcui80@hotmail.com.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, PR China. Electronic address: ruanhongjiang@yeah.net.
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Ruan
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D000068579:Celecoxib / Q000737:chemistry / Q000494:pharmacology; D002645:Chickens; D007773:Lactates / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D013710:Tendons / Q000473:pathology*; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*
chemical_list: D007773:Lactates; C058523:polyethylene oxide-polylactic acid block copolymer; D011092:Polyethylene Glycols; D000068579:Celecoxib
keywords: Adhesion prevention; Biomimetic tendon sheath; Celecoxib releasing; Electrospun fibrous membrane; Tendon sheath