title: Alteration of liver glycopatterns during cirrhosis and tumor progression induced by HBV.
abstract: The incidence of hepatocellular carcinoma (HCC) is closely correlated with hepatitis B virus (HBV)-induced liver cirrhosis. Structural changes in the glycans of serum and tissue proteins are reliable indicators of liver damage. However, little is known about the alteration of liver glycopatterns during cirrhosis and tumor progression induced by HBV infection. This study compared the differential expression of liver glycopatterns in 7 sets of normal pericarcinomatous tissues (PCTs), cirrhotic, and tumor tissues from patients with liver cirrhosis and HCC induced by HBV using lectin microarrays. Fluorescence-based lectin histochemistry and lectin blotting were further utilized to validate and assess the expression and distribution of certain glycans in 9 sets of corresponding liver tissue sections. Eight lectins (e.g., Jacalin and AAL) revealed significant difference in cirrhotic tissues versus PCTs. Eleven lectins (e.g., EEL and SJA) showed significant alteration during cirrhotic and tumor progression. The expression of Galα1-3(Fucα1-2)Gal (EEL) and fucosyltransferase 1 was mainly increasing in the cytoplasm of hepatocytes during PCTs-cirrhotic-tumor tissues progression, while the expression of T antigen (ACA and PNA) was decreased sharply in cytoplasm of tumor hepatocytes. Understanding the precision alteration of liver glycopatterns related to the development of hepatitis, cirrhosis, and tumor induced by HBV infection may help elucidate the molecular mechanisms underlying the progression of chronic liver diseases and develop new antineoplastic therapeutic strategies.
authors.affiliation: Department of Cell Biology and Genetics, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, People's Republic of China.|Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China.
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China.
authors.forename: Tianran
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Cell Biology and Genetics, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China.
authors.forename: Haoxiang
authors.initials: H
authors.lastname: Wu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Cell Biology and Genetics, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, People's Republic of China. hchen@mail.xjtu.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China. zhengli@nwu.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D005260:Female; D005355:Fibrosis / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D006023:Glycoproteins / Q000378:metabolism*; D006509:Hepatitis B / Q000378:metabolism* / Q000473:pathology; D006515:Hepatitis B virus; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D009363:Neoplasm Proteins
keywords: Glycopattern; HBV; HCC; Lectin microarray; Liver tissue
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