title: Enzymatic synthesis of ligand-bearing DNAs for metal-mediated base pairing utilising a template-independent polymerase.
abstract: We have developed a novel method to synthesise artificial ligand-bearing DNAs utilising a template-independent DNA polymerase. Hydroxypyridone ligand-bearing nucleotides () were successively appended to DNA primers by the enzyme. The resulting strands, tailed with nucleotides, formed Cu(II)-mediated metallo-DNA duplexes through the formation of metal-mediated artificial base pairs (H-Cu(II)-H).
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shionoya@chem.s.u-tokyo.ac.jp.
authors.forename: Teruki
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shionoya@chem.s.u-tokyo.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takezawa
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shionoya@chem.s.u-tokyo.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Sakamoto
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shionoya@chem.s.u-tokyo.ac.jp.
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Shionoya
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D008024:Ligands; D008670:Metals / Q000737:chemistry*; D013698:Templates, Genetic
chemical_list: D008024:Ligands; D008670:Metals; D004247:DNA; D004259:DNA-Directed DNA Polymerase