title: Neuroprotective effect of curcumin-loaded lactoferrin nano particles against rotenone induced neurotoxicity.
abstract: Curcumin is known to have neuroprotective role and possess antioxidant, anti-inflammatory activities. Rotenone, a flavonoid induced neurotoxicity in dopaminergic cells is being widely studied in Parkinson's Disease (PD) research. In the present study, curcumin loaded lactoferrin nano particles prepared by sol-oil chemistry were used to protect dopaminergic cell line SK-N-SH against rotenone induced neurotoxicity. These curcumin loaded nano particles were of 43-60 nm diameter size and around 100 nm hydrodynamic size as assessed by transmission electron microscopy, atomic force microscopy and dynamic light scattering analysis respectively. The encapsulation efficiency was 61.3% ± 2.4%. Cellular uptake of curcumin through these nano particles was confirmed by confocal imaging and spectrofluorimetric analysis. The curcumin loaded lactoferrin nanoparticles showed greater intracellular drug uptake, sustained retention and greater neuroprotection than soluble counterpart. Neuroprotective activity was characterized through viability assays and by estimating ROS levels. Furthermore rotenone induced PD like features were characterized by decrease in tyrosine hydroxylase expression and increase in α-synuclein expression. Taken together curcumin loaded lactoferrin nanoparticles could be a promising drug delivery strategy against neurotoxicity in dopaminergic neurons.
authors.affiliation: Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, India.
authors.forename: V Satish
authors.initials: VS
authors.lastname: Bollimpelli
authors.affiliation: Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, India. Electronic address: akondapi@gmail.com.
authors.forename: Anand K
authors.initials: AK
authors.lastname: Kondapi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D003474:Curcumin / Q000008:administration & dosage* / Q000138:chemical synthesis; D004339:Drug Compounding; D006801:Humans; D007781:Lactoferrin / Q000008:administration & dosage* / Q000138:chemical synthesis; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000138:chemical synthesis; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity*
chemical_list: D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D012402:Rotenone; D007781:Lactoferrin; D003474:Curcumin
keywords: Curcumin; Lactoferrin; Nano particle; Rotenone