title: Immunolocalization of the Anti-Müllerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre-antral Follicles Enclosed in Ovarian Tissue.
abstract: The aims of this study were to evaluate the localization, by immunohistochemistry, of the anti-Müllerian hormone (AMH) in goat ovaries and to investigate its effects on the in vitro survival and development of caprine pre-antral follicles enclosed in fragments of ovarian tissue. Pre-antral follicles were cultured in vitro for 1 or 7 days in α-MEM(+) in the absence or presence of kit ligand (KL; 50 ng/ml, positive control) or AMH (50 or 150 ng/ml). The results showed that AMH was localized in oocytes and granulosa cells from the primordial follicle to antral follicle stages. Addition of AMH maintained the percentage of developing follicles, similar to that in the uncultured control; however, the percentage of developing follicles was significantly lower than that in the cultured control and KL. Nonetheless, addition of AMH to the culture medium did not affect survival rates and follicular growth. In conclusion, this study demonstrated that the expression of AMH varies according to the compartment and stage of follicular development. Furthermore, AMH inhibits the activation of caprine primordial follicles.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
authors.forename: R M P
authors.initials: RM
authors.lastname: Rocha
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Carvalho
authors.affiliation: Laboratory Morphofunctional, Health Sciences Center, University of Fortaleza, Fortaleza-CE, Brazil.
authors.forename: R N
authors.initials: RN
authors.lastname: Chaves
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: M P
authors.initials: MP
authors.lastname: Bernuci
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: A C J S
authors.initials: AC
authors.lastname: Rosa-e-Silva
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D053938:DNA Fragmentation; D005260:Female; D006041:Goats; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D021381:Protein Transport; D046509:Tissue Culture Techniques
chemical_list: D054304:Anti-Mullerian Hormone