title: Polymerase specific error rates and profiles identified by single molecule sequencing.
abstract: DNA polymerases have an innate error rate which is polymerase and DNA context specific. Historically the mutational rate and profiles have been measured using a variety of methods, each with their own technical limitations. Here we used the unique properties of single molecule sequencing to evaluate the mutational rate and profiles of six DNA polymerases at the sequence level. In addition to accurately determining mutations in double strands, single molecule sequencing also captures direction specific transversions and transitions through the analysis of heteroduplexes. Not only did the error rates vary, but also the direction specific transitions differed among polymerases.
authors.affiliation: Department of Human Genetics, KU Leuven, O&N I Herestraat 49-box 602, 3000 Leuven, Belgium.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Hestand
authors.affiliation: Department of Human Genetics, KU Leuven, O&N I Herestraat 49-box 602, 3000 Leuven, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Van Houdt
authors.affiliation: Department of Human Genetics, KU Leuven, O&N I Herestraat 49-box 602, 3000 Leuven, Belgium.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cristofoli
authors.affiliation: Department of Human Genetics, KU Leuven, O&N I Herestraat 49-box 602, 3000 Leuven, Belgium. Electronic address: joris.vermeesch@uzleuven.be.
authors.forename: Joris R
authors.initials: JR
authors.lastname: Vermeesch
mesh_terms: D020029:Base Pairing; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011687:Purines; D011743:Pyrimidines; D017422:Sequence Analysis, DNA / Q000379:methods; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism
chemical_list: D009692:Nucleic Acid Heteroduplexes; D011687:Purines; D011743:Pyrimidines; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; C030986:pyrimidine; C030985:purine
keywords: Heteroduplex; Polymerase fidelity; Single molecule sequencing