title: Predictive role of miR-146a rs2910164 (C>G), miR-149 rs2292832 (T>C), miR-196a2 rs11614913 (T>C) and miR-499 rs3746444 (T>C) in the development of hepatocellular carcinoma.
abstract: We conducted a case-control study to evaluate the association of miR-146a rs2910164 (C>G), miR-149 rs2292832 (T>C), miR-196a2 rs11614913 (T>C) and miR-499 rs3746444 (T>C) polymorphisms with the risk of hepatocellular carcinoma. A total of 274 patients with HCC were collected between January 2013 and December 2014. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was taken to determine the polymorphism of miR-146a C>G, miR-149 T>C, miR-196a2 T>C and miR-499 T>C. By comparing with control groups, patients with HCC were more likely to be males (OR=2.01, 95% CI=1.38-2.95), have older age (OR=1.52, 95% CI=1.09-2.13), have a history of alcohol drinking (OR=2.09, 95% CI=1.49-2.93), and be infected with HBV (OR=32.98, 95% CI=19.70-55.46) and HCV (OR=56.26, 95% CI=23.28-152.98) infection. By conditional regression analysis, individuals carrying the TC and CC genotypes of miR-196a2 T>C were found to be associated with an elevated risk of HCC compared to the TT genotype, and the adjusted odds ratio were 1.50 (1.03-2.17) and 2.86 (1.60-5.16), respectively. Moreover, the TC+CC genotype was correlated with an increased risk of HCC (OR=1.69, 95% CI=1.19-2.41) compared to the wide-type genotype. In conclusion, our results suggested that miR-196a2 T>C polymorphism is associated with HCC risk in Chinese population.
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Yan
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Miaojuan
authors.initials: M
authors.lastname: Xia
authors.affiliation: Henan Provincial Hospital Number 7 Weiwu Road, Zhengzhou, Henan 450003, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Guanxiu
authors.initials: G
authors.lastname: Tang
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Geriatrics, The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.affiliation: Department of Neurology of The Third Xiangya Hospital, Central South University 138 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Gong
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C542542:MIRN146 microRNA, human; C551557:MIRN149 microRNA, human; C542543:MIRN196 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs
keywords: MiR-146a C>G; hepatocellular carcinoma; miR-149 T>C; miR-196a2 T>C; miR-499 T>C; polymorphism
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