title: Expression and functional role of miR-29b in renal cell carcinoma.
abstract: OBJECTIVE
  microRNAs (miRNAs) play essential roles in many tumors, including renal cell carcinoma (RCC). The aim of the present study was to investigate the expression and functional role of miR-29b in RCC and to identify its target genes.
  
  
  METHODS
  We determined the expression of miR-29b in clear cell RCC (ccRCC) tissues and RCC cell lines (786-O, A498, and SN12-PM6) using quantitative real-time PCR (qRT-PCR). The associations between miR-29b expression and clinical pathological parameters and prognosis were explored. Besides, the role of miR-29b in the SN12-PM6 cells proliferation, apoptosis, cycle, and invasion were investigated after transduction with lentivirus vectors. The kines in family member 1B (KIF1B), possible miR-29b target genes, were predicted using bioinformatics approaches, as well as the role in the pathogenesis of RCC.
  
  
  RESULTS
  Elevated expression of miR-29b was found in both tumor tissues and cell lines. High expression of miR-29b was significantly associated with tumor-node-metastasis (TNM) stage (P = 0.026) and the overall survival (P = 0.009) in the ccRCC. Inhibition of miR-29b expression could promote apoptosis, and inhibit proliferation and invasion ability in SN12-PM6 cells. Also, we confirmed that miR-29b could directly regulate the expression of KIF1B at the post transcriptional level.
  
  
  CONCLUSIONS
  These data suggest that miR-29b acts as an oncomiR, promoting proliferation and invasion ability through KIF1B suppression, and it might be a potential marker for prognosis of RCC.
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Zhangming
authors.initials: Z
authors.lastname: Lei
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wan
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.
authors.forename: Yanyue
authors.initials: Y
authors.lastname: Tong
mesh_terms: D000368:Aged; D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019295:Computational Biology; D030541:Databases, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016547:Kinesins / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D014162:Transfection
chemical_list: C091053:KIF1B protein, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D016547:Kinesins
keywords: Renal cell carcinoma; kinesin family member 1B; miR-29b
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