title: First record of the rare species Aeromonas schubertii from mussels: phenotypic and genetic reevaluation of the species and a review of the literature.
abstract: In a study where the prevalence of Aeromonas in shellfish was analysed, three isolates of Aeromonas schubertii were identified, representing this the first report of this species from mussels. This species was originally described in 1988 from strains isolated from extra-intestinal human infections and since then has been cited in only 18 occasions. For many years, A. schubertii was the only mannitol-negative species of the genus. However, three additional mannitol-negative species (Aeromonas simiae, Aeromonas diversa and Aeromonas australiensis) have been described. This, together with the fact that A. schubertii is a rare human pathogenic species, motivated the present study to characterize its biochemical behaviour and differentiation from the other mannitol-negative species. The molecular similarity (16S rRNA, rpoD and gyrB genes) of the strains, presence of virulence genes and antimicrobial resistance were determined. All A. schubertii strains showed the same phenotypic behaviour, i.e. they use citrate, are positive for lysine decarboxylase and DL-lactate, but negative for production of mannitol, indole and acid from sucrose and could be easily differentiated from other mannitol-negative species. All strains carried the aerA and lafA virulence genes and showed susceptibility to all antibiotics tested. Seafood could be a transmission route of this bacterium to humans.
authors.affiliation: Unidad de Microbiología, Facultad de Medicina y Ciencias de la Salud, IISPV, Universidad Rovira i Virgili, Sant Llorenç 21, 43201, Reus, Spain.
authors.forename: Fadua
authors.initials: F
authors.lastname: Latif-Eugenín
authors.affiliation: Unidad de Microbiología, Facultad de Medicina y Ciencias de la Salud, IISPV, Universidad Rovira i Virgili, Sant Llorenç 21, 43201, Reus, Spain.
authors.forename: Roxana
authors.initials: R
authors.lastname: Beaz-Hidalgo
authors.affiliation: Unidad de Microbiología, Facultad de Medicina y Ciencias de la Salud, IISPV, Universidad Rovira i Virgili, Sant Llorenç 21, 43201, Reus, Spain. mariajose.figueras@urv.cat.
authors.forename: María José
authors.initials: MJ
authors.lastname: Figueras
mesh_terms: D000333:Aeromonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D049872:Bivalvia / Q000382:microbiology*; D002262:Carboxy-Lyases / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D027081:DNA Gyrase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D012808:Sigma Factor; D019343:Citric Acid; D019344:Lactic Acid; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D027081:DNA Gyrase
keywords: Aeromonas schubertii; Antimicrobials; Molecular and phenotypic identification; Mussels; Virulence genes