title: Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicin-induced acute kidney injury.
abstract: The therapeutic action of umbilical cord-derived mesenchymal stem cells (UC-MSCs) against acute kidney injury (AKI) has been demonstrated by several groups. However, how to further enhance the renoprotective effect of UC-MSCs and improve the therapy effect, are still unclear. In this study, we mainly investigated whether insulin-like growth factor-1 (IGF-1)-modified UC-MSCs hold an enhanced protective effect on gentamicin-induced AKI in vivo. Our results indicated that the IGF-1 overexpression could enhance the therapeutic action of human UC-MSCs, and the AKI rats treated with IGF-1-overexpressed UC-MSCs (UC-MSCs-IGF-1) showed better recovery of biochemical variables in serum or urine associated with renal function, histological injury and renal apoptosis, compared with AKI rats treated with normal UC-MSCs. RNA microarray analysis indicated that some key genes in the signal pathways associated with anti-oxidation, anti-inflammatory, and cell migratory capacity were up-regulated in UC-MSCs-IGF-1, and the results were further confirmed with qPCR. Furthermore, a series of detection in vitro and in vivo indicated that the UC-MSCs-IGF-1 hold better anti-oxidation, anti-inflammatory, and cell migratory capacity for IGF-1 overexpression. Thus, our study indicated that enhancement of UC-MSCs bioactivities with IGF-1 overexpression could increase the UC-MSCs therapeutic potential and further developed a new therapeutic strategy for the treatment of AKI.
authors.affiliation: Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, P.R. China.|Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, P.R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China.
authors.forename: Yetong
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, P.R. China.
authors.forename: Delu
authors.initials: D
authors.lastname: Dong
authors.affiliation: Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, P.R. China.|Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, P.R. China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, P.R. China.|Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, P.R. China.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, P.R. China.
authors.forename: Yulai
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015415:Biomarkers; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D020869:Gene Expression Profiling; D005839:Gentamicins / Q000009:adverse effects; D006801:Humans; D016130:Immunophenotyping; D018836:Inflammation Mediators / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D000954:Antigens, Surface; D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D005839:Gentamicins; D018836:Inflammation Mediators; D007334:Insulin-Like Growth Factor I
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