title: Unique β-Glucuronidase Locus in Gut Microbiomes of Crohn's Disease Patients and Unaffected First-Degree Relatives.
abstract: Crohn's disease, an incurable chronic inflammatory bowel disease, has been attributed to both genetic predisposition and environmental factors. A dysbiosis of the gut microbiota, observed in numerous patients but also in at least one hundred unaffected first-degree relatives, was proposed to have a causal role. Gut microbiota β-D-glucuronidases (EC 3.2.1.33) hydrolyse β-D-glucuronate from glucuronidated compounds. They include a GUS group, that is homologous to the Escherichia coli GusA, and a BG group, that is homologous to metagenomically identified H11G11 BG and has unidentified natural substrates. H11G11 BG is part of the functional core of the human gut microbiota whereas GusA, known to regenerate various toxic products, is variably found in human subjects. We investigated potential risk markers for Crohn's disease using DNA-sequence-based exploration of the β-D-glucuronidase loci (GUS or Firmicute H11G11-BG and the respective co-encoded glucuronide transporters). Crohn's disease-related microbiomes revealed a higher frequency of a C7D2 glucuronide transporter (12/13) compared to unrelated healthy subjects (8/32). This transporter was in synteny with the potential harmful GUS β-D-glucuronidase as only observed in a Eubacterium eligens plasmid. A conserved NH2-terminal sequence in the transporter (FGDFGND motif) was found in 83% of the disease-related subjects and only in 12% of controls. We propose a microbiota-pathology hypothesis in which the presence of this unique β-glucuronidase locus may contribute to an increase risk for Crohn's disease.
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Gloux
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors.forename: Jamila
authors.initials: J
authors.lastname: Anba-Mondoloni
mesh_terms: D000328:Adult; D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D003424:Crohn Disease / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D064806:Dysbiosis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000378:metabolism; D005051:Eubacterium / Q000145:classification / Q000235:genetics / Q000378:metabolism; D005190:Family; D005260:Female; D000068536:Bacillota / Q000145:classification / Q000235:genetics / Q000378:metabolism; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D056426:Genetic Loci; D020723:Glucuronic Acid / Q000378:metabolism; D005966:Glucuronidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020719:Glucuronides / Q000378:metabolism; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012307:Risk Factors; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D020719:Glucuronides; D026901:Membrane Transport Proteins; D020723:Glucuronic Acid; D005966:Glucuronidase
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