title: [An LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in human urine and application to urinary excretion study].
abstract: The study aims to develop an LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in urine samples, and to investigate their urinary excretion characteristics in healthy volunteers after intravenous infusion administration of Huoxue-Tongluo lyophilized powder for injection (HTLPI). The urine samples were extracted by methanol, and then separated on a Hedera ODS-2 column with a mobile phase of acetonitrile and 5 mmol · L(-1) ammonium acetate buffer solution containing 0.05% formic acid (20:80). Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited good linearity over the concentration range of 0.03 -40 µg · mL(-1). The values on both the occasions (intra- and inter-day) were all within 15% at three concentration levels. No matrix effect and carry-over effect were observed. Amygdalin and paeoniflorin were stable in human urine under different storage conditions. Approximately 79.6% of the administered amount of amygdalin was excreted unchanged in urine within 24 h and which was 48.4% for paeoniflorin. The developed LC-MS/MS method can be applied to evaluate the urinary excretion of amygdalin and paeoniflorin.
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Li
authors.forename: Fu-guo
authors.initials: FG
authors.lastname: Shi
authors.forename: Ling-yan
authors.initials: LY
authors.lastname: Jian
authors.forename: Li
authors.initials: L
authors.lastname: Ding
mesh_terms: D000678:Amygdalin / Q000652:urine*; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal; D005960:Glucosides / Q000652:urine*; D006801:Humans; D039821:Monoterpenes / Q000652:urine*; D053719:Tandem Mass Spectrometry; D006027:Glycosides
chemical_list: D000678:Amygdalin; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C527174:tongluo; C015423:peoniflorin; D006027:Glycosides