title: Purification and Characterization of a Novel Cold-Active Lipase from the Yeast Candida zeylanoides.
abstract: Cold-active lipases have attracted attention in recent years due to their potential applications in reactions requiring lower temperatures. Both bacterial and fungal lipases have been investigated, each having distinct advantages for particular applications. Among yeasts, cold-active lipases from the genera Candida, Yarrowia, Rhodotorula, and Pichia have been reported. In this paper, biosynthesis and properties of a novel cold-active lipase from Candida zeylanoides isolated from refrigerated poultry meat are described. Heat-sterilized olive oil was found to be the best lipase biosynthesis inducer, while nonionic detergents were not effective. The enzyme was purified to homogeneity using hydrophobic chromatography and its enzymatic properties were tested. Pure enzyme activity at 7 °C was about 60% of the maximal activity at 27 °C. The enzyme had rather good activity at higher temperatures, as well. Optimal pH of pure lipase was between 7.3 and 8.2, while the enzyme from the crude extract had an optimum pH of about 9.0. The enzyme was sensitive to high ionic strength and lost most of its activity at high salt concentrations. Due to the described properties, cold-active C. zeylanoides lipase has comparative advantages to most similar enzymes with technological applications and may have potential to become an industrially important enzyme.
authors.affiliation: Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
authors.forename: Iva
authors.initials: I
authors.lastname: Čanak
authors.forename: Adrienn
authors.initials: A
authors.lastname: Berkics
authors.forename: Nikolett
authors.initials: N
authors.lastname: Bajcsi
authors.forename: Monika
authors.initials: M
authors.lastname: Kovacs
authors.forename: Agnes
authors.initials: A
authors.lastname: Belak
authors.forename: Renata
authors.initials: R
authors.lastname: Teparić
authors.forename: Anna
authors.initials: A
authors.lastname: Maraz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mrša
mesh_terms: D002175:Candida / Q000201:enzymology*; D003080:Cold Temperature; D003902:Detergents / Q000494:pharmacology; D004789:Enzyme Activation; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D008460:Meat / Q000382:microbiology; D000069463:Olive Oil / Q000494:pharmacology; D013696:Temperature
chemical_list: D003902:Detergents; D005656:Fungal Proteins; D000069463:Olive Oil; D008049:Lipase