title: Responses of Soil Bacterial Communities to Nitrogen Deposition and Precipitation Increment Are Closely Linked with Aboveground Community Variation.
abstract: It has been predicted that precipitation and atmospheric nitrogen (N) deposition will increase in northern China; yet, ecosystem responses to the interactive effects of water and N remain largely unknown. In particular, responses of belowground microbial community to projected global change and their potential linkages to aboveground macro-organisms are rarely studied. In this study, we examined the responses of soil bacterial diversity and community composition to increased precipitation and multi-level N deposition in a temperate steppe in Inner Mongolia, China, and explored the diversity linkages between aboveground and belowground communities. It was observed that N addition caused the significant decrease in bacterial alpha-diversity and dramatic changes in community composition. In addition, we documented strong correlations of alpha- and beta-diversity between plant and bacterial communities in response to N addition. It was found that N enriched the so-called copiotrophic bacteria, but reduced the oligotrophic groups, primarily by increasing the soil inorganic N content and carbon availability and decreasing soil pH. We still highlighted that increased precipitation tended to alleviate the effects of N on bacterial diversity and dampen the plant-microbe connections induced by N. The counteractive effects of N addition and increased precipitation imply that even though the ecosystem diversity and function are predicted to be negatively affected by N deposition in the coming decades; the combination with increased precipitation may partially offset this detrimental effect.
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.
authors.forename: Zhuwen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.|College of Environmental Science, Shenyang University, Shenyang, 110044, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, Institute for Bioinformatics and Evolutionary Studies (IBEST), University of Idaho, Moscow, ID, 83844, USA.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Top
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.
authors.forename: Ruzhen
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Environmental Science, Shenyang University, Shenyang, 110044, China.
authors.forename: Yuge
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jiangping
authors.initials: J
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Han
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang, 110016, China. jiangyong@iae.ac.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D044822:Biodiversity; D002244:Carbon / Q000378:metabolism / Q000494:pharmacology; D011232:Chemical Precipitation; D002681:China; D002980:Climate; D017753:Ecosystem; D059013:Microbial Consortia; D009584:Nitrogen / Q000737:chemistry* / Q000378:metabolism; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D014867:Water / Q000494:pharmacology
chemical_list: D012987:Soil; D014867:Water; D002244:Carbon; D009584:Nitrogen
keywords: Aboveground-belowground linkages; Copiotroph/oligotroph model; Global change; Interactive effects of nitrogen deposition and precipitation increment; Soil bacterial diversity and community composition; Temperate steppe
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