title: TruSPAdes: barcode assembly of TruSeq synthetic long reads.
abstract: The recently introduced TruSeq synthetic long read (TSLR) technology generates long and accurate virtual reads from an assembly of barcoded pools of short reads. The TSLR method provides an attractive alternative to existing sequencing platforms that generate long but inaccurate reads. We describe the truSPAdes algorithm (http://bioinf.spbau.ru/spades) for TSLR assembly and show that it results in a dramatic improvement in the quality of metagenomics assemblies.
authors.affiliation: Center for Algorithmic Biotechnology, Institute for Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.
authors.forename: Anton
authors.initials: A
authors.lastname: Bankevich
authors.affiliation: Center for Algorithmic Biotechnology, Institute for Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.|Department of Computer Science and Engineering, University of California at San Diego, La Jolla, California, USA.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Pevzner
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
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