title: Differential proteomics analysis of mononuclear cells in cerebrospinal fluid of Parkinson's disease.
abstract: Parkinson's disease (PD) is one common neurodegenerative disease featured with degeneration of dopaminergic neurons in substantia nigra. Multiple factors participate in the pathogenesis and progression of PD. In this study, we investigated the proteomics profiles of mononuclear cells in cerebrospinal fluids from both PD patients and normal people, in order to explore the correlation between disease factors and PD. Cerebrospinal fluid samples were collected from both PD and normal people and were separated for mononuclear cells in vitro. Proteins were then extracted and separated by 2-dimensional gel electrophoresis. Proteins with differential expressions were identified by comparison to standard proteome expression profile map, followed by software and database analysis. In PD patients, there were 8 proteins with consistent expression profile and 16 proteins with differential expressions. Those differential proteins identified include cytoskeleton proteins (actin, myosin), signal transduction proteins (adenosine cyclase binding protein 1, calcium binding protein, talin) and anti-oxidation factor (thioredoxin peroxide reductase). PD patients had differential protein expressional profiles in the mononuclear cells of cerebrospinal fluids compared to normal people, suggesting the potential involvement of cytoskeleton and signal transduction proteins in apoptosis of neuronal apoptosis and PD pathogenesis.
authors.affiliation: Department of Neurology, Inner Mongolia North Heavy Industries Group Corp. Ltd Hospital, (Third Affiliated Hospital of Baotou Medical College) Baotou 014030, Inner Mongolia, China.
authors.forename: Lifei
authors.initials: L
authors.lastname: Xing
authors.affiliation: Department of Thoracic Surgery, The Central Hospital Baotou 014040, Inner Mongolia, China.
authors.forename: Dongtao
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Image, Inner Mongolia North Heavy Industries Group Corp. Ltd Hospital, (Third Affiliated Hospital of Baotou Medical College) Baotou 014030, Inner Mongolia, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Image, Inner Mongolia North Heavy Industries Group Corp. Ltd Hospital, (Third Affiliated Hospital of Baotou Medical College) Baotou 014030, Inner Mongolia, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Lan
authors.affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Suyue
authors.initials: S
authors.lastname: Pan
mesh_terms: D015415:Biomarkers / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D002555:Cerebrospinal Fluid / Q000737:chemistry* / Q000166:cytology; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins
keywords: Parkinson’s disease; cerebrospinal fluid; mononuclear cells; proteomics
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