title: Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer.
abstract: A new biomolecular quantitation method, nanoparticle-mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich-type assay system were transformed into a near-infrared (NIR) laser-driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive biomolecular quantitation using a thermometer. This is the first report of biomolecular quantitation using a thermometer and also serves as the first attempt to introduce the nanoparticle-mediated photothermal effect for bioassays.
authors.affiliation: Department of Chemistry, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA. xli4@utep.edu.
authors.forename: Guanglei
authors.initials: G
authors.lastname: Fu
authors.affiliation: Department of Chemistry, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA. xli4@utep.edu.
authors.forename: Sharma T
authors.initials: ST
authors.lastname: Sanjay
authors.affiliation: Department of Chemistry, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA. xli4@utep.edu.
authors.forename: Maowei
authors.initials: M
authors.lastname: Dou
authors.affiliation: Department of Chemistry, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA. xli4@utep.edu and Biomedical Engineering, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA and Border Biomedical Research Center, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA.
authors.forename: XiuJun
authors.initials: X
authors.lastname: Li
mesh_terms: D001681:Biological Assay; D002138:Calibration; D005290:Ferric Compounds / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared; D013821:Thermometers
chemical_list: D005290:Ferric Compounds; D005295:Ferrocyanides; C000499:ferric oxide; D017430:Prostate-Specific Antigen; C000170:ferric ferrocyanide
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