title: Potential Biomarker Peptides Associated with Acute Alcohol-Induced Reduction of Blood Pressure.
abstract: The purpose of this study was to explore the peptides that are related to acute reduction of blood pressure after alcohol drinking. Venous blood was collected from male healthy volunteers before and after drinking white wine (3 ml/kg weight) containing 13% of ethanol. Peptidome analysis for serum samples was performed using a new target plate, BLOTCHIP®. Alcohol caused significant decreases in systolic and diastolic blood pressure levels at 45 min. The peptidome analysis showed that the levels of three peptides of m/z 1467, 2380 and 2662 changed significantly after drinking. The m/z 1467 and 2662 peptides were identified to be fragments of fibrinogen alpha chain, and the m/z 2380 peptide was identified to be a fragment of complement C4. The intensities of the m/z 2380 and m/z 1467 peptides before drinking were associated with % decreases in systolic and diastolic blood pressure levels at 45 min after drinking compared with the levels before drinking, while there were no significant correlations between the intensity of the m/z 2662 peptide and % decreases in systolic and diastolic blood pressure levels after drinking. The m/z 1467 and 2380 peptides are suggested to be markers for acute reduction of blood pressure after drinking alcohol.
authors.affiliation: Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Wakabayashi
authors.affiliation: Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Marumo
authors.affiliation: Membrane Protein and Ligand Analysis Center, Protosera Inc., Amagasaki, Hyogo, 660-0083, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nonaka
authors.affiliation: Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shimomura
authors.affiliation: Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Eguchi
authors.affiliation: Membrane Protein and Ligand Analysis Center, Protosera Inc., Amagasaki, Hyogo, 660-0083, Japan.
authors.forename: Lyang-Ja
authors.initials: LJ
authors.lastname: Lee
authors.affiliation: Membrane Protein and Ligand Analysis Center, Protosera Inc., Amagasaki, Hyogo, 660-0083, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Legal Medicine, Nara Medical University, Kashihara, Nara, 634-8521, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hatake
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects* / Q000502:physiology; D001795:Blood Pressure Determination; D003181:Complement C4 / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D014920:Wine
chemical_list: D015415:Biomarkers; D003181:Complement C4; D010446:Peptide Fragments; C464794:fibrinogen Aalpha; D000431:Ethanol; D005340:Fibrinogen
references.citation: Public Health Rep. 1989 Nov-Dec;104(6):615-20
references.pmid: 2511595
references.citation: Alcohol Clin Exp Res. 1994 Feb;18(1):105-10
references.pmid: 8198204
references.citation: J Hypertens Suppl. 1983 Dec;1(2):97-9
references.pmid: 6400122
references.citation: Alcohol Clin Exp Res. 1991 Jun;15(3):478-82
references.pmid: 1877732
references.citation: Addiction. 2009 Dec;104(12):1981-90
references.pmid: 19804464
references.citation: Pharmacol Biochem Behav. 1990 Feb;35(2):437-42
references.pmid: 2320652
references.citation: J Pharmacol Exp Ther. 1987 Jan;240(1):59-64
references.pmid: 3100772
references.citation: Br Heart J. 1983 Feb;49(2):174-82
references.pmid: 6824540
references.citation: Clin Exp Pharmacol Physiol. 2001 Dec;28(12):1020-4
references.pmid: 11903307
references.citation: Dis Markers. 2010;29(1):11-20
references.pmid: 20826913
references.citation: Alcohol Clin Exp Res. 1989 Apr;13(2):229-31
references.pmid: 2658661
references.citation: Biochem Biophys Res Commun. 2009 Jan 30;379(1):110-4
references.pmid: 19073144
references.citation: Pharmacol Biochem Behav. 1983;18 Suppl 1:127-33
references.pmid: 6356156
references.citation: Proteomics Clin Appl. 2009 Jul;3(7):821-8
references.pmid: 21136989
references.citation: PLoS One. 2011;6(10):e26540
references.pmid: 22046305
references.citation: Clin Sci (Lond). 1984 Jun;66(6):659-63
references.pmid: 6373096
references.citation: J Cardiovasc Risk. 2003 Feb;10(1):21-4
references.pmid: 12569233
references.citation: IEEE/ACM Trans Comput Biol Bioinform. 2004 Jul-Sep;1(3):98-108
references.pmid: 17048385
references.citation: Proteomics. 2011 Jul;11(13):2727-37
references.pmid: 21630454
references.citation: Arterioscler Thromb. 1992 Jul;12(7):780-8
references.pmid: 1616903
references.citation: BMJ. 1999 Dec 11;319(7224):1523-8
references.pmid: 10591709
references.citation: Alcohol Clin Exp Res. 1998 Oct;22(7):1409-13
references.pmid: 9802521
references.citation: JAMA. 2014 Feb 5;311(5):507-20
references.pmid: 24352797
references.citation: Nat Rev Mol Cell Biol. 2002 Jul;3(7):509-19
references.pmid: 12094217
references.citation: Hypertension. 1992 Aug;20(2):219-26
references.pmid: 1639464
references.citation: Alcohol Alcohol. 2002 Sep-Oct;37(5):409-15
references.pmid: 12217928
references.citation: Fed Proc. 1982 Jun;41(8):2447-51
references.pmid: 7044829
references.citation: Addiction. 2000 Oct;95(10):1505-23
references.pmid: 11070527
references.citation: Arch Intern Med. 2006 Dec 11-25;166(22):2437-45
references.pmid: 17159008