title: Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
abstract: To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
  
  
  
  The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE. A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
  
  
  
  In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure. Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
  
  
  
  The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China; Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, Guizhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Jiang Fan
authors.initials: JF
authors.lastname: Xiu
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Jin Zhi
authors.initials: JZ
authors.lastname: Cheng
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Man
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Xiao Li
authors.initials: XL
authors.lastname: Shang
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Guizhou Medical University, Guiyang 550004, Guizhou, China.
authors.forename: Jian Wei
authors.initials: JW
authors.lastname: Wu
mesh_terms: D000818:Animals; D002686:Chitin / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism*; D006793:Houseflies / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000378:metabolism*; D040901:Proteomics
chemical_list: D019476:Insect Proteins; D002686:Chitin
keywords: Musca domestica; Peritrophic matrix; Proteome