title: Transcriptome modifications in human gingival fibroblasts exposed to 2-hydroxyethyl methacrylate.
abstract: 2-Hydroxyethyl methacrylate (HEMA),a tooth filling material, was proven to have toxic effects on different cell types, including human gingival fibroblasts (HGFs), and to be able to influence odontoblast vitality. The aim of the present study was to assess the differential transcriptome modulation induced by low HEMA concentration in cultured HGFs. RNA extracted from cultured HGFs exposed to 3 mmol/l HEMA for 24 or 96 h underwent a whole genome microarray analysis. Data analysis showed the presence of two gene clusters, composed by 310 transcripts differentially expressed after 24- and 96-h HEMA treatment compared to controls. Functional analysis demonstrated that these transcripts are mainly involved in cellular survival and death, and inflammatory response. The study highlighted an overall damage induced by HEMA exposure at both 24 and 96 h, mainly leading to a proliferation impairment. Interestingly, 24-h HEMA treatment seems to induce the cells to trigger repair mechanisms, evidencing an early compensatory response, whereas 96-h incubation appears to cause the occurrence of apoptosis as a consequence of the chronic damage.
authors.affiliation: Department of Pharmacy, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy. Electronic address: c.dinisio@unich.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Nisio
authors.affiliation: Department of Psychological, Health and Territorial Sciences, School of Medicine and Health Sciences, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy; Center of Excellence on Aging, "G.d'Annunzio" University, Via L. Polacchi, 66100 Chieti-Pescara, Italy. Electronic address: m.daurora@unich.it.
authors.forename: Marco
authors.initials: M
authors.lastname: D'Aurora
authors.affiliation: Department of Pharmacy, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy. Electronic address: v.digiacomo@unich.it.
authors.forename: Viviana
authors.initials: V
authors.lastname: di Giacomo
authors.affiliation: Department of Psychological, Health and Territorial Sciences, School of Medicine and Health Sciences, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy; Center of Excellence on Aging, "G.d'Annunzio" University, Via L. Polacchi, 66100 Chieti-Pescara, Italy. Electronic address: stuppia@unich.it.
authors.forename: Liborio
authors.initials: L
authors.lastname: Stuppia
authors.affiliation: Department of Pharmacy, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy. Electronic address: cataldi@phobos.unich.it.
authors.forename: Amelia
authors.initials: A
authors.lastname: Cataldi
authors.affiliation: Department of Psychological, Health and Territorial Sciences, School of Medicine and Health Sciences, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy; Center of Excellence on Aging, "G.d'Annunzio" University, Via L. Polacchi, 66100 Chieti-Pescara, Italy. Electronic address: v.gatta@unich.it.
authors.forename: Valentina
authors.initials: V
authors.lastname: Gatta
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003764:Dental Materials / Q000009:adverse effects* / Q000627:therapeutic use; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008689:Methacrylates / Q000009:adverse effects* / Q000627:therapeutic use; D046228:Microarray Analysis; D014176:Protein Biosynthesis / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
chemical_list: D003764:Dental Materials; D008689:Methacrylates; D017382:Reactive Oxygen Species; C005044:hydroxyethyl methacrylate
keywords: 2-Hydroxyethyl methacrylate; Apoptosis; Gene expression; Human gingival fibroblasts; Microarray