title: MiR-152 regulates metastases of non-small cell lung cancer cells by targeting neuropilin-1.
abstract: MicroRNAs (miRNAs) are a class of small non-coding RNAs that have been suggested to play critical roles in tumorigenesis. Recently, miR-152 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-152 in non-small cell lung cancer (NSCLC) is still unclear. In the present study, our findings showed that the expression of miR-152 was significantly down-regulated and neuropilin-1 was up-regulated in the NSCLC specimens. Moreover, the levels of miR-152 and neuropilin-1 were inversely correlated. Bioinformatics analyses and luciferase reporter assay showed that miR-152 targeted the 3'-UTR of neuropilin-1 mRNA to inhibit its translation. Furthermore, overexpression of miR-152 inhibited neuropilin-1 mediated cell invasiveness, while down-regulated expression of miR-152 increased neuropilin-1 mediated cell invasiveness in NSCLC cells. Together, these findings indicated that miR-152 suppression in NSCLC cells might promote neuropilin-1 mediated cancer metastasis and suggested a new therapeutic application of miR-152 in the treatment of NSCLC.
authors.affiliation: Department of Respiratory Medicine, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Xiao-Cun
authors.initials: XC
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Respiratory Medicine, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D039942:Neuropilin-1 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C550856:MIRN152 microRNA, human; D035683:MicroRNAs; D039942:Neuropilin-1
keywords: Non-small cell lung cancer; invasion; miR-152; migration; neuropilin-1
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