title: Flavonoid and phenolic acid profile by LC-MS/MS and biological activity of crude extracts from Chenopodium hybridum aerial parts.
abstract: Extracts from leaves and stems of Chenopodium hybridum were characterised for the presence and quantity of flavonoids and phenolic acids by LC-ESI-MS/MS. Five flavonoids and eight phenolic acids were detected for the first time in aerial parts of this plant species, the most abundant compounds being rutin (2.80 μg/g DW), 3-kaempferol rutinoside (2.91 μg/g DW), 4-OH-benzoic (1.86 μg/g DW) and syringic acids (2.31 μg/g DW). Extracts were tested for anti-inflammatory/antiarthritic, antihyaluronidase and cytotoxic activities against human prostate cancer (Du145, PC3) and melanoma cell lines (A375, HTB140 and WM793) of different malignancy. None of the extracts protected bovine serum albumin from heat-induced denaturation. Antihyaluronidase effect at the tested concentration was higher than standard naringenin. Cytotoxic activity was generally low with an exception of the extract from the leaves, which was found most effective against prostate Du145 cell line with 98.28 ± 1.13% of dead cells at 100 μg/mL.
authors.affiliation: a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.
authors.forename: I
authors.initials: I
authors.lastname: Podolak
authors.affiliation: b Department of Pharmaceutical Botany , Medical University of Lublin , Lublin , Poland.
authors.forename: M
authors.initials: M
authors.lastname: Olech
authors.affiliation: a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.
authors.forename: A
authors.initials: A
authors.lastname: Galanty
authors.affiliation: a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.
authors.forename: D
authors.initials: D
authors.lastname: Załuski
authors.affiliation: a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.
authors.forename: K
authors.initials: K
authors.lastname: Grabowska
authors.affiliation: a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.
authors.forename: D
authors.initials: D
authors.lastname: Sobolewska
authors.affiliation: c Faculty of Biochemistry, Biophysics and Biotechnology, Department of Cell Biology , Jagiellonian University , Cracow , Poland.
authors.forename: M
authors.initials: M
authors.lastname: Michalik
authors.affiliation: b Department of Pharmaceutical Botany , Medical University of Lublin , Lublin , Poland.
authors.forename: R
authors.initials: R
authors.lastname: Nowak
mesh_terms: D045744:Cell Line, Tumor; D027462:Chenopodium / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid
keywords: Chenopodium hybridum; LC-MS/MS; antidenaturation activity; antihyaluronidase; cytotoxic activity; flavonoids; phenolic acids