title: Genomic characterization of Pasteurella multocida HB01, a serotype A bovine isolate from China.
abstract: Pasteurella multocida infects various domestic and feral animals, generally causing clinical disease. To investigate P. multocida disease in cattle, we sequenced the complete genome of P. multocida HB01 (GenBank accession CP006976), a serotype A organism isolated from a cow in China. The genome is composed of a single circular chromosome of 2,416,068 base pairs containing 2212 protein-coding sequences, 6 ribosomal rRNA operons, and 56 tRNA genes. The present study confirms that P. multocida HB01 possesses a more complete metabolic pathway with an intact trichloroacetic acid cycle for anabolism compared with A. pleuropneumoniae and Haemophilus parasuis. This is the first time that this metabolic mechanism of P. multocida has been described. We also identified a full spectrum of genes related to known virulence factors of P. multocida. The differences in virulence factors between strains of different serotypes and origins were also compared. This comprehensive comparative genome analysis will help in further studies of the metabolic pathways, genetic basis of serotype, and virulence of P. multocida.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: pengzhong525@163.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, The Cooperative Innovation Center for Sustainable Pig Production, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wanleung828@webmail.hzau.edu.cn.
authors.forename: Wan
authors.initials: W
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wenjing-918114@163.com.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wub@mail.hzau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai 200000, China. Electronic address: tb_411@163.com.
authors.forename: Biao
authors.initials: B
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: tanchen@mail.hzau.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: rzhou@mail.hzau.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: chenhch@mail.hzau.edu.cn.
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002681:China; D016680:Genome, Bacterial; D008070:Lipopolysaccharides / Q000378:metabolism; D016979:Pasteurella multocida / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides
keywords: Comparative analysis; Genome sequencing; Metabolism; Pasteurella multocida; Virulence factors