title: Insights into the subunit arrangement and diversity of paradoxin and taipoxin.
abstract: Paradoxin and taipoxin are neurotoxic phospholipases from the inland and coastal species of Australian taipan. Despite their relatively high sequence homology of 70% and 84% for the acidic and basic chains respectively, they differ substantially in reported assays of neurotoxicity. This study provides the first characterisation of paradoxin, which like taipoxin, is a trimer at physiological pH. More broadly, these toxins were found to be composed of a more diverse range of subunits than previously recognised, including newly discovered γTPx isoforms, which give rise to an additional, major conformation of TPx.
authors.affiliation: Illawarra Health and Medical Research Institute, University of Wollongong, Northfields Avenue, Wollongong NSW, 2522, Australia; School of Biological Sciences, University of Wollongong, Northfields Avenue, Wollongong NSW, 2522, Australia. Electronic address: jah994@uowmail.edu.au.
authors.forename: Julian A
authors.initials: JA
authors.lastname: Harrison
authors.affiliation: Illawarra Health and Medical Research Institute, University of Wollongong, Northfields Avenue, Wollongong NSW, 2522, Australia; School of Biological Sciences, University of Wollongong, Northfields Avenue, Wollongong NSW, 2522, Australia. Electronic address: andrew.aquilina1@uowmail.edu.au.
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Aquilina
mesh_terms: D000818:Animals; D001315:Australia; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004546:Elapid Venoms / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D017815:Elapidae / Q000378:metabolism*; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009498:Neurotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D054497:Phospholipases A2, Secretory / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D030162:Reptilian Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004546:Elapid Venoms; D007527:Isoenzymes; D009498:Neurotoxins; D021122:Protein Subunits; D030162:Reptilian Proteins; C521070:paradoxin; C015523:taipoxin; D054497:Phospholipases A2, Secretory
keywords: Elapids; Mass spectrometry; Structure; Toxin; Venom