title: Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes.
abstract: West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.
authors.affiliation: From the Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan, Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-Ku, Tokyo 162-8640, Japan.
authors.forename: Tadaki
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: From the Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kawaguchi
authors.affiliation: From the Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors.forename: Wallaya
authors.initials: W
authors.lastname: Phongphaew
authors.affiliation: Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshii
authors.affiliation: Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimo-Kateau, Chuo, Yamanashi 409-3898, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Iwano
authors.affiliation: Department of Cell Biology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan, and.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Harada
authors.affiliation: Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.affiliation: From the Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Orba
authors.affiliation: From the Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan, Global Institution for Collaborative Research and Education (GI-CoRE) and Global Virus Network (GVN), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan h-sawa@czc.hokudai.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
mesh_terms: D000818:Animals; D001692:Biological Transport; D002522:Chlorocebus aethiops; D011992:Endosomes / Q000201:enzymology* / Q000821:virology; D005347:Fibroblasts / Q000201:enzymology / Q000821:virology; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D021381:Protein Transport; D022161:Transport Vesicles / Q000821:virology; D014709:Vero Cells; D014764:Viral Proteins; D057074:Virus Release; D014779:Virus Replication; D014902:West Nile virus / Q000502:physiology*; D020691:rab GTP-Binding Proteins / Q000502:physiology*
chemical_list: D014764:Viral Proteins; C576245:Rab8b protein, mouse; D020691:rab GTP-Binding Proteins
keywords: Rab; flavivirus; infection; transport; vesicles
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