title: Kinetic model of metabolic network for xiamenmycin biosynthetic optimisation.
abstract: Xiamenmycins, a series of prenylated benzopyran compounds with anti-fibrotic bioactivities, were isolated from a mangrove-derived Streptomyces xiamenensis. To fulfil the requirements of pharmaceutical investigations, a high production of xiamenmycin is needed. In this study, the authors present a kinetic metabolic model to evaluate fluxes in an engineered Streptomyces lividans with xiamenmycin-oriented genetic modification based on generic enzymatic rate equations and stability constraints. Lyapunov function was used for a viability optimisation. From their kinetic model, the flux distributions for the engineered S. lividans fed on glucose and glycerol as carbon sources were calculated. They found that if the bacterium can utilise glucose simultaneously with glycerol, xiamenmycin production can be enhanced by 40% theoretically, while maintaining the same growth rate. Glycerol may increase the flux for phosphoenolpyruvate synthesis without interfering citric acid cycle. They therefore believe this study demonstrates a possible new direction for bioengineering of S. lividans.
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Min-juan
authors.initials: MJ
authors.lastname: Xu
authors.affiliation: SmartWin Technology, 67 Tranmere Ave, Carnegie, VIC, Melbourne 3163, Australia.
authors.forename: Yong-cong
authors.initials: YC
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. aoping@sjtu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Ao
authors.affiliation: GeneMath, 5525 27th Ave. N.E., Seattle, WA 98105, USA.
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Zhu
mesh_terms: D001578:Benzopyrans; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D013302:Streptomyces / Q000378:metabolism; D049490:Systems Biology; D013912:Threonine / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D001578:Benzopyrans; C582113:N-((3,4-dihydro-3-hydroxy-2-methyl-2-(4'-methyl-3'-pentenyl)-2H-1-benzopyran-6-yl)carbonyl)threonine; D013912:Threonine
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