title: Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae).
abstract: OBJECTIVE
  Polymorphic microRNA (miRNA)-based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae).
  
  
  RESULTS
  A total of 485 miRNA-based microsatellites were found from the genomic DNA sequences of N. nucifera. After several rounds of screening, 21 primer pairs flanking di-, tri-, or pentanucleotide repeats were identified that revealed high levels of genetic diversity in four populations with two to five alleles per locus. The observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.803, respectively.
  
  
  CONCLUSIONS
  The polymorphic microsatellite markers will be useful for studying the genetic diversity and population structure of N. nucifera.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Songtao
authors.initials: S
authors.lastname: Gui
authors.affiliation: Hubei Province Engineering Research Center of Legume Plants, School of Life Sciences, Jianghan University, Wuhan 430056, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
keywords: Nelumbo nucifera; Nelumbonaceae; genetic diversity; microRNA (miRNA); microsatellites; polymorphism
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