title: On the regulative role of the glutamate receptor in mitochondria.
abstract: The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was shown to be suppressed by γ-aminobutyric acid (GABA). The GABA receptor presence in the heart mitochondria was shown by golding with the use of antibodies to α- and β-subunits of the receptor. The activity of glutamate receptor was assessed according to the rate of synthesis of hydrogen peroxide. The glutamate receptor in mitochondria could be activated only under conditions of hypoxic stress, which in model experiments was imitated by blocking Complex I by rotenone or fatty acids. The glutamate signal in mitochondria was shown to be calcium- and potential-dependent and the activation of the glutamate cascade was shown to be accompanied by production of hydrogen peroxide. It was discovered that H2O2 synthesis involves two complexes of the mitochondrial electron transfer system - succinate dehydrogenase (SDH) and fatty acid dehydrogenase (ETF:QO). Thus, functions of the glutamate signaling system are associated with the system of respiration-glycolysis switching (the Pasteur-Crabtree) under conditions of hypoxia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Selin
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Lobysheva
authors.forename: Semen V
authors.initials: SV
authors.lastname: Nesterov
authors.forename: Yulia A
authors.initials: YA
authors.lastname: Skorobogatova
authors.forename: Ivan M
authors.initials: IM
authors.lastname: Byvshev
authors.forename: Lyubov L
authors.initials: LL
authors.lastname: Pavlik
authors.forename: Irina B
authors.initials: IB
authors.lastname: Mikheeva
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Moshkov
authors.forename: Lev S
authors.initials: LS
authors.lastname: Yaguzhinsky
authors.forename: Yaroslav R
authors.initials: YR
authors.lastname: Nartsissov
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D019069:Cell Respiration; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors / Q000378:metabolism; D044927:Electron-Transferring Flavoproteins / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006019:Glycolysis; D006861:Hydrogen Peroxide / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism; D018080:Receptors, GABA-B / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism*; D015398:Signal Transduction; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D044927:Electron-Transferring Flavoproteins; D007506:Iron-Sulfur Proteins; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D006861:Hydrogen Peroxide; D013385:Succinate Dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C023120:electron-transferring-flavoprotein dehydrogenase; D042967:Electron Transport Complex I