title: Extraction and purification of IgG by hydrophilic organic solvent salting-out extraction.
abstract: The distribution of IgG in different salting-out extraction systems (SOESs), including ethanol/K2HPO4, Na2CO3, and trisodium citrate, was investigated. The SOESs that were composed of 20% K2HPO4 (w/w)-14% ethanol (w/w), 19% Na2CO3 (w/w)-13% ethanol (w/w) and 25% trisodium citrate (w/w)-19% ethanol (w/w) showed satisfactory results, with IgG recovery rates as high as 97.4%, 93.1%, and 90.2%, respectively. ELISA, CD and IR analyses confirmed the active retention and structural constant of IgG in the K2HPO4-ethanol system. An optimized system that consisted of 14% ethanol (w/w)-20% K2HPO4 (w/w) (pH 7.0) was selected for extracting active plasma proteins directly from pig plasma. As a result, recovery rates as high as 95.7% for IgG and 93.0% for albumin were achieved. In addition, some contaminating proteins were also removed by this system, which may provide a new alternative method for the separation and purification of immunoglobulin.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 116024, PR China. Electronic address: yshdong@dlut.edu.cn.
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Dong
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 116024, PR China.
authors.forename: Bofeng
authors.initials: B
authors.lastname: Pang
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 116024, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 116024, PR China.
authors.forename: LingLing
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 116024, PR China.
authors.forename: Wencai
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 116024, PR China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Xiu
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification*; D012965:Sodium Chloride / Q000737:chemistry*; D013552:Swine
chemical_list: D007074:Immunoglobulin G; D000431:Ethanol; D012965:Sodium Chloride
keywords: Active retention; Immune globulin; Purification; Recovery; Salting-out extraction systems; Structural constant