title: Degeneration of stria vascularis in age-related hearing loss; a corrosion cast study in a mouse model.
abstract: Conclusion With age, in a mouse model, degenerative changes to the capillaries of the stria vascularis are observed. These range from a narrowing of vessel lumen to complete degeneration of strial vessels. Other vascular beds in the cochlea are relatively unchanged with age. Strial capillaries at the cochlear base are significantly more affected than those in mid-apical turns. Objectives Previous work suggests that age-related hearing loss is associated with degenerative changes to cochlear vasculature; the term strial presbyacusis is often cited. This study reports on vascular changes observed in a murine model of presbyacusis, analyzed using corrosion cast techniques. Methods A novel corrosion cast technique was developed to compare cochlear vasculature in control mice (non-presbycusic, CD1) and old (> 6 months) C57BL/6 animals. ABR measures indicated a significant age-related threshold elevation in the C57BL/6 mice. Cochlear vascular casts were imaged using scanning electron microscopy, and vessel degeneration was quantified by measuring capillary diameters. Results Corrosion casts of cochlear vasculature in 6-12 month old C57BL/6 mice reveal significant degeneration of stria vascularis. Other capillary beds (spiral ligament and the spiral limbus) appear unchanged. Comparison of strial capillary diameters reveals significantly more damage in basal/lower-turn regions compared with the cochlear mid-turn.
authors.affiliation: a Auditory Science Laboratory, Program in Neuroscience and Mental Health, and Department of Otolaryngology - HNS , the Hospital for Sick Children , Canada ;|b Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto , Toronto , Canada ;
authors.forename: Mattia
authors.initials: M
authors.lastname: Carraro
authors.affiliation: a Auditory Science Laboratory, Program in Neuroscience and Mental Health, and Department of Otolaryngology - HNS , the Hospital for Sick Children , Canada ;|b Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto , Toronto , Canada ;|c Department of Otolaryngology - Head & Neck Surgery , University of Toronto , Toronto , Canada.
authors.forename: Robert V
authors.initials: RV
authors.lastname: Harrison
mesh_terms: D000818:Animals; D016319:Corrosion Casting / Q000379:methods*; D004195:Disease Models, Animal; D008810:Mice, Inbred C57BL; D010477:Perfusion; D011304:Presbycusis / Q000473:pathology*; D013316:Stria Vascularis / Q000473:pathology*
keywords: C57BL/6 mouse; CD1 mouse; Presbyacusis; cochlear hearing loss; organ of Corti; scanning electron microscopy; spiral ligament; strial presbyacusis