title: Brain cortex mitochondrial bioenergetics in synaptosomes and non-synaptic mitochondria during aging.
abstract: Alterations in mitochondrial bioenergetics have been associated with brain aging. In order to evaluate the susceptibility of brain cortex synaptosomes and non-synaptic mitochondria to aging-dependent dysfunction, male Swiss mice of 3 or 17 months old were used. Mitochondrial function was evaluated by oxygen consumption, mitochondrial membrane potential and respiratory complexes activity, together with UCP-2 protein expression. Basal respiration and respiration driving proton leak were decreased by 26 and 33 % in synaptosomes from 17-months old mice, but spare respiratory capacity was not modified by aging. Succinate supported state 3 respiratory rate was decreased by 45 % in brain cortex non-synaptic mitochondria from 17-month-old mice, as compared with young animals, but respiratory control was not affected. Synaptosomal mitochondria would be susceptible to undergo calcium-induced depolarization in 17 months-old mice, while non-synaptic mitochondria would not be affected by calcium overload. UCP-2 was significantly up-regulated in both synaptosomal and submitochondrial membranes from 17-months old mice, compared to young animals. UCP-2 upregulation seems to be a possible mechanism by which mitochondria would be resistant to suffer oxidative damage during aging.
authors.affiliation: Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina. slarnaiz@ffyb.uba.ar.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lores-Arnaiz
authors.affiliation: Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
authors.forename: Paulina
authors.initials: P
authors.lastname: Lombardi
authors.affiliation: Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
authors.forename: Analía G
authors.initials: AG
authors.lastname: Karadayian
authors.affiliation: Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
authors.forename: Federico
authors.initials: F
authors.lastname: Orgambide
authors.affiliation: Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
authors.forename: Daniela
authors.initials: D
authors.lastname: Cicerchia
authors.affiliation: Centro de Altos Estudios en Ciencias de la Salud, Universidad Abierta Interamericana, Buenos Aires, Argentina.
authors.forename: Juanita
authors.initials: J
authors.lastname: Bustamante
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004734:Energy Metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D002118:Calcium
keywords: Aging; Cerebral cortex; Depolarization; Non-synaptic mitochondria; Respiration; Synaptosomes
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