title: A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase.
abstract: Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene (glu1923) and a glycoside hydrolase gene (glu1392). Hydrolytic experiments revealed that the combined effect of Glu1923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli (E. coli) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a Ki value of 2.25M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study.
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Gao
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China.
authors.forename: An
authors.initials: A
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China.
authors.forename: Bo-Liang
authors.initials: BL
authors.lastname: Gong
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Li
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China.
authors.forename: Yu-Huan
authors.initials: YH
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China. Electronic address: lsshezm@mail.sysu.edu.cn.
authors.forename: Zhu-Mei
authors.initials: ZM
authors.lastname: He
authors.affiliation: School of Life Sciences, Sun Yat-Sen Univeristy, Guangzhou 510275, P.R. China. Electronic address: lsslig@mail.sysu.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D001709:Biotechnology; D004795:Enzyme Stability; D015723:Gene Library; D049750:Genome, Insect; D005959:Glucosidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020049:Isoptera / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D054892:Metagenome; D005810:Multigene Family; D010770:Phosphotransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D019476:Insect Proteins; D011994:Recombinant Proteins; D010770:Phosphotransferases; D005959:Glucosidases
keywords: Coptotermes formosanus; Glucose tolerance; Glucosidase; Metagenomic library; Phosphotransferase system