title: Small interfering RNA expression inhibits avian infectious bronchitis virus replication and inflammatory response.
abstract: Avian infectious bronchitis virus (IBV) is a major cause of poor weight gain and mortality among chicks.
  
  
  
  A lentivirus vector was used to generate transgenic chickens expressing small interfering RNA (siRNA) targeting the M protein of IBV. Offspring of generation 0 (G0) were screened to identify G1 transgenic chickens (Tg). Monocytes from G1 Tg were stimulated with IBV in vitro.
  
  
  
  Monocytes producing siRNA efficiently inhibit IBV replication. Expression of inflammatory cytokines, Mx protein and nitric oxide levels were lower in early IBV infection in Tg. In vivo experiments show that siRNA expression inhibits IBV replication, significantly decreases mortality and increases weight gain. Inflammatory responses and oxidative damage were significantly decreased, yielding minimal tissue injury. The inflammatory responses indicate that the cellular immune response is most effective during the initial stage, while the humoral immune response is more significant in later stages of infection.
  
  
  
  Small interfering RNA expression inhibits avian IBV replication and inflammatory response.
authors.affiliation: National Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.|Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Shoulong
authors.initials: S
authors.lastname: Deng
authors.affiliation: Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.affiliation: National Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.|Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Zhengxing
authors.initials: Z
authors.lastname: Lian
authors.affiliation: National Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens / Q000821:virology; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D001351:Infectious bronchitis virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D007249:Inflammation / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D008264:Macrophages / Q000276:immunology / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology*; D034741:RNA, Small Interfering / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering