title: Use of aquaporins 1 and 5 levels as a diagnostic marker in mild-to-moderate adult-onset asthma.
abstract: Characteristic features of asthma include airway inflammation and hyperactivity, mucus hypersecretion, mucosal edema, and airway remodeling. These features could be due to pathological water transport across pulmonary epithelia and aquaporins (AQPs) have recently been isolated as key proteins in fluid transportation in the human respiratory tract. We aimed to evaluate the role of aquaporins in the pathogenesis of asthma and their possible use a diagnostic marker of the disease. A total of 110 hospitalized and outpatients with mild to moderate adult-onset asthma were invited to participate in this study and 34 submitted an induced sputum sample adequate for analysis. The amount of AQP1, AQP5 and MUC5AC were measured with ELISA assay. The amount of IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ and IL-17 in both serum and sputum were measured with Cytometry Bead Array (CBA kit). Our results suggest that sputum AQP5, AQP1 and MUC5AC are all in a good correlation (r=0.498 between AQP5 and AQP1, r=0.529 and r=0.661 between MUC5AC and AQP5 or AQP1, respectively, all P<0.05). The AUC value for AQP1 and AQP5 to diagnose asthma were 0.729 and 0.745, respectively. In conclusion, water homeostasis plays an important role in maintaining adequate fluid transportation within the lung and is involved in the pathogenesis of asthma. Our results suggest that AQP may influence pulmonary physiology that their dysfunction can contribute to pulmonary pathogenesis, such as asthma. Moreover, their quantification could serve as biomarkers for the diagnosis of asthma.
authors.affiliation: Department of Respiratory Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gong
authors.affiliation: Xinjiang Laboratory of Respiratory Disease Research, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Bilal
authors.initials: B
authors.lastname: Hasan
authors.affiliation: Xinjiang Laboratory of Respiratory Disease Research, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Respiratory Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, China.
authors.forename: Fengsen
authors.initials: F
authors.lastname: Li
mesh_terms: D000328:Adult; D017668:Age of Onset; D051398:Aquaporin 1 / Q000032:analysis*; D051402:Aquaporin 5 / Q000032:analysis*; D019540:Area Under Curve; D001249:Asthma / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008168:Lung / Q000737:chemistry* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000032:analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D012720:Severity of Illness Index; D013183:Sputum / Q000737:chemistry
chemical_list: C496020:AQP1 protein, human; C496036:AQP5 protein, human; D051402:Aquaporin 5; D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D051398:Aquaporin 1
keywords: Asthma; aquaporin 1; aquaporin 5; disease models; humans
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