title: Resistance Level of Mosquito Species (Diptera: Culicidae) from Shandong Province, China.
abstract: This study describes the aquatic habitats, species composition, and the insecticide resistance level of the mosquito Culex pipiens pallens in Shandong Province, China. A cross-sectional survey of mosquito larval habitats was conducted from May to November 2014 to determine the species composition and larval abundance. Larvae were collected using the standard dipping technique, and a total of four habitat types were sampled. The fourth instar larvae of Cx. pipiens pallens collected in each habitat type were tested for resistance to five insecticides according to a WHO bioassay. A total of 7,281 mosquito larvae were collected, of which 399 (5.48%) were categorized as Anopheles mosquito larvae (An. sinensis), 6636 (91.14%) as culicine larvae (Cx. pipiens pallens, Cx. tritaeniorhynchus, Cx. halifaxii, and Cx. bitaeniorhynchus), 213 (2.93%) as Armigeres larvae, and 33 (0.45%) as Aedes larvae (Aedes albopictus). In addition, a total of 1,149 mosquito pupae were collected. Culex larvae were distributed in all habitats investigated. Tukeys HSD analysis showed that roadside drainages were the most productive habitat type for Culex larvae. Armigeres species were found only in drains, Aedes only in water tanks, and Anopheles in water that was comparatively clear and rich in emergent plants. Bioassay showed that the maximum resistance level of Cx. pipiens pallens was to deltamethrin, while it was lowest to plifenate. The productivity of various mosquitoes in different habitat types is very heterogeneous. It is particularly important to modify human activity and the environment to achieve effective mosquito vector control. For effective larval control, the type of habitat should be considered, and the most productive habitat type should be given priority in mosquito abatement programs.
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Liu
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Yang
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Hai-Fang
authors.initials: HF
authors.lastname: Wang
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Liu
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Zhao
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Huai-Wei
authors.initials: HW
authors.lastname: Wang
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Chong-Xing
authors.initials: CX
authors.lastname: Zhang
authors.affiliation: Department of Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.
authors.forename: Mao-Qing
authors.initials: MQ
authors.lastname: Gong
keywords: mosquito; resistance level
references.citation: Acta Trop. 2008 Jan;105(1):67-73
references.pmid: 18068136
references.citation: Malar J. 2008 Jan 25;7:20
references.pmid: 18218148
references.citation: Trop Med Int Health. 2003 Jan;8(1):37-47
references.pmid: 12535249
references.citation: Am J Trop Med Hyg. 2008 Jul;79(1):19-27
references.pmid: 18606759
references.citation: J Vector Ecol. 2014 Jun;39(1):78-82
references.pmid: 24820559
references.citation: Trends Parasitol. 2009 Jul;25(7):319-27
references.pmid: 19559649
references.citation: PLoS One. 2011 Apr 29;6(4):e19473
references.pmid: 21559301
references.citation: Am J Trop Med Hyg. 2008 Dec;79(6):866-75
references.pmid: 19052295
references.citation: Mol Med Rep. 2013 Feb;7(2):525-30
references.pmid: 23151871
references.citation: Am J Trop Med Hyg. 2005 Sep;73(3):546-52
references.pmid: 16172479
references.citation: PLoS Pathog. 2010 Aug 05;6(8):e1001000
references.pmid: 20700451
references.citation: Mol Phylogenet Evol. 2001 May;19(2):317-25
references.pmid: 11341813
references.citation: PLoS Negl Trop Dis. 2015 Apr 07;9(4):e0003702
references.pmid: 25849040
references.citation: PLoS One. 2010 Jul 21;5(7):e11681
references.pmid: 20657783
references.citation: PLoS One. 2012;7(7):e41145
references.pmid: 22815944
references.citation: J Vector Borne Dis. 2007 Mar;44(1):44-51
references.pmid: 17378216