title: c-Abl contributes to glucose-promoted apoptosis via p53 signaling pathway in podocytes.
abstract: OBJECTIVE
  To investigate the role of the non-receptor tyrosine kinase c-Abl in high glucose-induced podocyte injury and its possible signal transduction pathway.
  
  
  METHODS
  Sixteen C57BL/6 mice were randomly assigned to a group with diabetes and a normal control group. Subsequently, differentiated mouse podocytes were exposed to high-glucose conditions, and podocyte apoptosis was then assessed by flow cytometry and Hoechst 33258 staining. Western blot and immunofluorescence assay were used to measure c-Abl expression. Co-immunoprecipitation assay was used and c-Abl siRNA was applied to evaluate the interaction between c-Abl and p53.
  
  
  RESULTS
  High glucose promotes podocyte apoptosis. The c-Abl expression in podocytes was increased after exposure to high glucose, stimulating the p53 signaling pathway. Conversely, treatment with c-Abl siRNA restored high glucose-promoted podocyte apoptosis and resulted in the reduction of p53 expression.
  
  
  CONCLUSIONS
  c-Abl contributes to high glucose-induced podocyte apoptosis via p53 signaling pathway.
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei, China.
authors.forename: Yiqiong
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Chen
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liang
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei, China. Electronic address: ghxding@gmail.com.
authors.forename: Guohua
authors.initials: G
authors.lastname: Ding
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D005947:Glucose; D006943:Hyperglycemia / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D050199:Podocytes / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D034741:RNA, Small Interfering; D011897:Random Allocation; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D016315:Proto-Oncogene Proteins c-abl; D005947:Glucose
keywords: Apoptosis; High glucose; Podocytes; c-Abl