title: [Screening differentially expressed plasma proteins in cold stress rats based on iTRAQ combined with mass spectrometry technology].
abstract: OBJECTIVE
  Isobaric tags for relative and absolute quantitation (iTRAQ) combined with mass spectrometry were used to screen differentially expressed plasma proteins in cold stress rats.
  
  
  METHODS
  Thirty health SPF Wistar rats were randomly divided into cold stress group A and control group B, then A and B were randomly divided into 3 groups (n = 5): A1, A2, A3 and B1, B2, B3. The temperature of room raising was (24.0 +/- 0.1) degrees C, and the cold stress temperature was (4.0 +/- 0.1) degrees C. The rats were treated with different temperatures until 12 h. The abdominal aortic blood was collected with heparin anticoagulation suction tube. Then, the plasma was separated for protein extraction, quantitative, enzymolysis, iTHAQ labeling, scx fractionation and mass spectrometry analysis.
  
  
  RESULTS
  Totally, 1085 proteins were identified in the test, 39 differentially expressed proteins were screened, including 29 up-regulated proteins and 10 down-regulated proteins. Three important differentially expressed proteins related to cold stress were screened by bioinfonnatics analysis (Minor histocompatihility protein HA-1, Has-related protein Rap-1b, Integrin beta-1).
  
  
  CONCLUSIONS
  In the experiment, the differentially expressed plasma proteins were successfully screened in cold stress rats. iTRAQ technology provided a good platform to screen protein diaguostic markers on cold stress rats, and laid a good foundation for further. study on animal cold stress mechanism.
authors.forename: Yan-zhi
authors.initials: YZ
authors.lastname: Liu
authors.forename: Jing-ru
authors.initials: JR
authors.lastname: Guo
authors.forename: Meng-ling
authors.initials: ML
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Zhen
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
authors.forename: Huan-min
authors.initials: HM
authors.lastname: Yang
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D003080:Cold Temperature; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological
chemical_list: D001798:Blood Proteins