title: [Variability of Three-dimensional Automatic Ovarian Follicle Count in Menstrual Cycle].
abstract: OBJECTIVE
  To evaluate the variability of three-dimensional automatic counts of ovarian follicles measuring 2-6 to 2-10 mm during the menstrual cycle and to determine if this test can be applied outside the early follicular phase of the menstrual cycle.
  
  
  METHODS
  in a prospective observational study, serial transvaginal ultrasound scans were performed from April 20, 2013, to October 30, 2014, on infertile patients.
  
  
  METHODS
  age between 18 and 35 years, BMI 18-25 kg/m2, regular menstrual cycles, no history of ovarian surgery and no hormonal changes in TSH, prolactin, fasting insulin or glucose. We excluded patients with ovarian cysts or who did not complete one or more days of the serial transvaginal ultrasound scans. The follicle count was performed in 3D mode ultrasound with a Sono AVC system. Visits were scheduled for the early follicular, mid-follicular, periovulatory and luteal phases of the menstrual cycle.
  
  
  RESULTS
  Forty-five women were included. The Friedman test showed that the total number of follicles measuring 2-6 mm varied significantly (p = 0.001) across the four periods of the menstrual cycle. The Paired Student t-test showed a significant increase in 2-6 mm follicle count from the mid-follicular and periovulatory phase to the luteal phase. We found no significant intra-cycle variation between the small follicles (2-6 mm) in the early follicular, mid-follicular and periovulatory phases. The Friedman test showed that the total number of follicles measuring 2-10 mm varied significantly (p = 0.003) across the menstrual cycle.
  
  
  CONCLUSIONS
  The variation of three-dimensional automatic counts of 2-6 mm follicles in the early follicular, mid-follicular and periovulatory phases was not statistically significant. The significant variability in the counts of follicles measuring 2-10 mm across the menstrual cycle does not permit this examination to be performed side the early follicular phase.
authors.affiliation: Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: Eduardo Camelo
authors.initials: EC
authors.lastname: de Castro
authors.affiliation: Faculdade de Medicina, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: Gercino
authors.initials: G
authors.lastname: Monteiro Filho
authors.affiliation: Faculdade de Medicina, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: Waldemar Naves
authors.initials: WN
authors.lastname: do Amaral
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D005640:Follicle Stimulating Hormone; D005498:Follicular Phase; D006801:Humans; D008183:Luteal Phase; D008597:Menstrual Cycle; D006080:Ovarian Follicle; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D005640:Follicle Stimulating Hormone
references.citation: Hum Reprod Update. 2014 Jan-Feb;20(1):124-40
references.pmid: 24077980
references.citation: Fertil Steril. 2013 May;99(6):1791-7
references.pmid: 23433830
references.citation: Ultrasound Obstet Gynecol. 2013 Feb;41(2):216-22
references.pmid: 22744915
references.citation: Fertil Steril. 2010 Aug;94(3):1044-51
references.pmid: 19589513
references.citation: Ultrasound Obstet Gynecol. 2010 Mar;35(3):354-60
references.pmid: 20069654
references.citation: Fertil Steril. 2008 Apr;89(4):927-33
references.pmid: 17603052
references.citation: Hum Reprod. 2007 Jul;22(7):1925-31
references.pmid: 17437960
references.citation: Hum Reprod. 2007 Mar;22(3):766-71
references.pmid: 17071823
references.citation: Hum Reprod. 2010 Jan;25(1):221-7
references.pmid: 19840990
references.citation: Hum Reprod. 2011 Jan;26(1):127-33
references.pmid: 21112951
references.citation: Ultrasound Obstet Gynecol. 2009 Apr;33(4):477-83
references.pmid: 19212944