title: Cyclin Y regulates the proliferation, migration, and invasion of ovarian cancer cells via Wnt signaling pathway.
abstract: This study is designated to investigate the roles of cyclin Y (CCNY) and Wnt signaling pathway in regulating ovarian cancer (OC) cell proliferation, migration, and invasion. Quantitative real-time PCR (qRT-PCR), Western blot, MTT assay, cell scratch, and transwell test were used in our study, and transplanted tumor model was constructed on nude mice. C-Myc, cyclin D1, PFTK1, ki67, OGT, and β-catenin protein expressions in tumor tissues were detected. CCNY was significantly upregulated in OC cell lines and tissues (both P < 0.05); significant association was observed between CCNY expression and clinicopathological stage, lymph node metastasis (LNM) (P < 0.05); and the CCNY expression in stages III to IV was higher than that in stages I to II, and patients with LNM had higher CCNY expression when compared with those in patients without LNM (P < 0.05); expressions of c-Myc, cyclin D, PFTK1, ki67, and OGT were upregulated in OC tissues compared with ovarian benign tissues, suggesting that these expressions were significantly different between the two groups (P < 0.05); CCNY significantly exacerbated proliferation, migration, and invasion of A2780 cells; c-Myc and cyclin D1 protein expressions increased as the expression of CCNY increased (P < 0.001); β-catenin expressions in A2780 cells with over-expression of CCNY were significantly increased in the nucleus, but significantly decreased in the cytoplasm (both P < 0.05); high expressions of CCNY exacerbated the proliferation of A2780 cells in nude mice and significantly increased c-Myc, cyclin D1, PFTK1, ki67, and OGT protein expressions in tumor tissues which were transplanted into nude mice (P < 0.01). CCNY might exacerbate the proliferation, migration, and invasion of OC cells via activating the Wnt signaling pathway. Thus, this study provides a theoretical foundation for the development of therapeutic drugs that are able to cure OC by targeting CCNY.
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Dongcheng District, Beijing, 100730, China. liuhaiyuan16@163.com.
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Dongcheng District, Beijing, 100730, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Dongcheng District, Beijing, 100730, China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Obstetrics and Gynecology, Linyi People's Hospital, Linyi, 276003, Shandong, People's Republic of China.
authors.forename: Xiangxiu
authors.initials: X
authors.lastname: Sun
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000556:secondary; D000328:Adult; D000368:Aged; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D016213:Cyclins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway
chemical_list: C543319:CCNY protein, human; D016213:Cyclins; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins
keywords: CCNY; Cyclin Y; Invasion; Migration; Ovarian cancer; Proliferation; Wnt signaling pathway; β-catenin
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