title: MicroRNA-195a-5p inhibits mouse medullary thymic epithelial cells proliferation by directly targeting Smad7.
abstract: MiR-195 has been implicated in inhibiting cell proliferation in different types of tumors. Whether it contributes to the process of thymic epithelial cells (TECs) proliferation remains unclear. In this study, we found that miR-195a-5p was highly up-regulated in the TECs isolated from the aging mice. Further experiments showed that miR-195a-5p mimic transfection inhibited the proliferation of mouse medullary thymic epithelial cell line 1 (MTEC1), whereas the transfection of miR-195a-5p inhibitor in MTEC1 had the opposite effect. In addition, miR-195a-5p had no obvious effect on MTEC1 apoptosis. Furthermore, Smad7, a negative regulator of transforming growth factor β pathway, was confirmed as a direct target of miR-195a-5p by luciferase assays. Taken together, our results indicate that miR-195a-5p inhibits MTEC1 proliferation, at least in part, via down-regulation of Smad7.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Dongguang
authors.initials: D
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Ye
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Qi
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China yuguli99@163.com liyugu@scau.edu.cn.
authors.forename: Yugu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D051906:Smad7 Protein / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism
chemical_list: C569389:MIRN195a microRNA, mouse; D035683:MicroRNAs; D051906:Smad7 Protein; C497807:Smad7 protein, mouse
keywords: cell proliferation, Smad7; miR-195a-5p; mouse thymic epithelial cell
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