title: Morphological Effects and Antioxidant Capacity of Solanum crispum (Natre) In Vitro Assayed on Human Erythrocytes.
abstract: In order to gain insight into the molecular mechanism of the antioxidant properties of Solanum crispum, aqueous extracts of its leaves were assayed on human erythrocytes and molecular models of its membrane. Phenolics and alkaloids were detected by HPLC-MS. Scanning electron and defocusing microscopy showed that S. crispum changed erythrocytes from the normal shape to echinocytes. These results imply that molecules present in the aqueous extracts were located in the outer monolayer of the erythrocyte membrane. Dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE) were chosen as representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. X-ray diffraction showed that S. crispum preferentially interacted with DMPC bilayers. Experiments regarding its antioxidant properties showed that S. crispum neutralized the oxidative capacity of HClO on DMPE bilayers; defocusing microscopy and hemolysis assays demonstrated the protective effect of S. crispum against the oxidant effects of HClO on human erythrocytes.
authors.affiliation: Faculty of Chemical Sciences, University of Concepción, Concepción, Chile. msuwalsk@udec.cl.
authors.forename: Mario
authors.initials: M
authors.lastname: Suwalsky
authors.affiliation: Faculty of Pharmacy, University of Concepción, Concepción, Chile.
authors.forename: Patricia
authors.initials: P
authors.lastname: Ramírez
authors.affiliation: Faculty of Pharmacy, University of Concepción, Concepción, Chile.
authors.forename: Marcia
authors.initials: M
authors.lastname: Avello
authors.affiliation: Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Fernando
authors.initials: F
authors.lastname: Villena
authors.affiliation: Center for Optics and Photonics, University of Concepción, Concepción, Chile.
authors.forename: María José
authors.initials: MJ
authors.lastname: Gallardo
authors.affiliation: Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile.
authors.forename: Andrés
authors.initials: A
authors.lastname: Barriga
authors.affiliation: Faculty of Exact and Natural Sciences, University of Antioquia, Medellín, Colombia.
authors.forename: Marcela
authors.initials: M
authors.lastname: Manrique-Moreno
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008051:Lipid Bilayers; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D032322:Solanum / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D008051:Lipid Bilayers; D010936:Plant Extracts; D059808:Polyphenols
keywords: Antioxidant activity; Erythrocyte membrane; Lipid bilayer; Polyphenols; Solanum crispum
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