title: Bovine serum albumin binding, antioxidant and anticancer properties of an oxidovanadium(IV) complex with luteolin.
abstract: Chemotherapy using metal coordination compounds for cancer treatment is the work of the ongoing research. Continuing our research on the improvement of the anticancer activity of natural flavonoids by metal complexation, a coordination compound of the natural antioxidant flavone luteolin (lut) and the oxidovanadium(IV) cation has been synthesized and characterized. Using different physicochemical measurements some structural aspects of [VO(lut)(H2O)2]Na·3H2O (VOlut) were determined. The metal coordinated to two cis-deprotonated oxygen atoms (ArO(-)) of the ligand and two H2O molecules. Magnetic measurements in solid state indicated the presence of an effective exchange pathway between adjacent vanadium ions. VOlut improved the antioxidant capacity of luteolin only against hydroxyl radical. The antitumoral effects were evaluated on MDAMB231 breast cancer and A549 lung cancer cell lines. VOlut exhibited higher viability inhibition (IC50=17 μM) than the ligand on MDAMB231 cells but they have the same behavior on A549 cells (ca. IC50=60 μM). At least oxidative stress processes were active during cancer cell-killing. When metals chelated through the carbonyl group and one adjacent OH group of the flavonoid an effective improvement of the biological properties has been observed. In VOlut the different coordination may be the cause of the small improvement of some of the tested properties of the flavonoid. Luteolin and VOlut could be distributed and transported in vivo. Luteolin interacted in the microenvironment of the tryptophan group of the serum binding protein, BSA, by means of electrostatic forces and its complex bind the protein by H bonding and van der Waals interactions.
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115-C.C.962-(B1900AVV), 1900 La Plata, Argentina.
authors.forename: Luciana G
authors.initials: LG
authors.lastname: Naso
authors.affiliation: Departamento de Química Inorgánica, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, P.O. Box 644, 48080 Bilbao, Spain; BCMaterials, Parque científico y Tecnológico de Bizkaia, Edificio 500-1, 48160 Derio, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Lezama
authors.affiliation: Innoprot SL, Parque científico y Tecnológico de Bizkaia, Edificio 502-P1, 48160 Derio, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Valcarcel
authors.affiliation: Innoprot SL, Parque científico y Tecnológico de Bizkaia, Edificio 502-P1, 48160 Derio, Spain.
authors.forename: Clarisa
authors.initials: C
authors.lastname: Salado
authors.affiliation: Innoprot SL, Parque científico y Tecnológico de Bizkaia, Edificio 502-P1, 48160 Derio, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Villacé
authors.affiliation: Innoprot SL, Parque científico y Tecnológico de Bizkaia, Edificio 502-P1, 48160 Derio, Spain.
authors.forename: Danel
authors.initials: D
authors.lastname: Kortazar
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115-C.C.962-(B1900AVV), 1900 La Plata, Argentina.
authors.forename: Evelina G
authors.initials: EG
authors.lastname: Ferrer
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115-C.C.962-(B1900AVV), 1900 La Plata, Argentina. Electronic address: williams@quimica.unlp.edu.ar.
authors.forename: Patricia A M
authors.initials: PA
authors.lastname: Williams
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D047311:Luteolin / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017968:Vanadium Compounds / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D017968:Vanadium Compounds; D012710:Serum Albumin, Bovine; D047311:Luteolin
keywords: BSA binding; Luteolin; Magnetic properties; Mechanisms of cytotoxic action; Oxidovanadium(IV) complexes