title: Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii - A forgotten endemic angiosperm of Turkey.
abstract: Context We report the first ever chemical/biochemical study on Crocus mathewii Kerndorff (Iridaceae) - a Turkish endemic angiosperm. This plant has never been explored for its phytochemistry and bioactivities. Objective This study explores C. mathewii corm and aerial parts for the chemical and biological properties of hexane, ethyl acetate, methanol and water fractions of the extracts. Material and methods Plant material (20 g) was extracted by methanol (250 mL × 5, 3 days each) and fractioned into hexane, ethyl acetate, methanol and water. All fractions were subjected to β-carotene-linoleic acid, DPPH(·), ABTS(·)(+), CUPRAC, metal chelating and tyrosinase inhibition activities. Hexane fractions were submitted to GC-MS analysis. Results Ethyl acetate fractions showed excellent IC50 values in DPPH(·) (aerial 36.21 ± 0.76 and corm 33.87 ± 0.02 mg/L) and ABTS(·)(+) (aerial 33.01 ± 0.79 and bulb 27.87 ± 0.33 mg/L); higher than the IC50 of the standard α-tocopherol (DPPH 116.25 ± 1.97; ABTS 52.64 ± 0.37 mg/L), higher than BHA in DPPH (57.31 ± 0.25 mg/L), but slightly lower in ABTS (19.86 ± 2.73 mg/L). Methanol extract of aerial parts also showed higher activity than α-tocopherol in DPPH (85.56 ± 11.51 mg/L) but slightly less (72.90 ± 3.66 mg/L) than both the standards in ABTS. Linoleic (aerial 53.9%, corm 43.9%) and palmitic (aerial 22.2%, corm 18%) were found as the major fatty acids. Discussion and conclusion Some fractions of C. mathewii showed higher antioxidant activities than the standards. There is a need to explore more about this plant.
authors.affiliation: a Department of Biology, Faculty of Science , Mugla Sıtkı Kocman University , Mugla , Turkey ;
authors.forename: Fatma
authors.initials: F
authors.lastname: Yildiztekin
authors.affiliation: b Department of Chemistry, Faculty of Science , Mugla Sıtkı Kocman University , Kötekli , Mugla , Turkey ;|c Department of Herbal and Animal Production , Koycegiz Vocational School, Mugla Sıtkı Kocman University , Mugla , Turkey.
authors.forename: Said
authors.initials: S
authors.lastname: Nadeem
authors.affiliation: b Department of Chemistry, Faculty of Science , Mugla Sıtkı Kocman University , Kötekli , Mugla , Turkey ;
authors.forename: Ebru
authors.initials: E
authors.lastname: Erol
authors.affiliation: c Department of Herbal and Animal Production , Koycegiz Vocational School, Mugla Sıtkı Kocman University , Mugla , Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Yildiztekin
authors.affiliation: a Department of Biology, Faculty of Science , Mugla Sıtkı Kocman University , Mugla , Turkey ;
authors.forename: Atilla L
authors.initials: AL
authors.lastname: Tuna
authors.affiliation: b Department of Chemistry, Faculty of Science , Mugla Sıtkı Kocman University , Kötekli , Mugla , Turkey ;
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozturk
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000302:isolation & purification / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D027622:Crocus / Q000737:chemistry; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000302:isolation & purification / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019308:Palmitic Acid / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D014421:Turkey
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D002614:Chelating Agents; D002800:Cholinesterase Inhibitors; D005227:Fatty Acids; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D019308:Palmitic Acid; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D014442:Monophenol Monooxygenase
keywords: ABTS; CUPRAC; DPPH; GC–MS analysis; β-carotene