title: The inhibitory effect of propofol on Kv2.1 potassium channel in rat parietal cortical neurons.
abstract: Excessive K(+) efflux via activated voltage-gated K(+) channels can deplete intracellular K(+) and lead to long-lasting membrane depolarization which will promote neuronal apoptosis during ischemia/hypoxia injury. The Kv2.1 potassium channel was the major component of delayed rectifier potassium current (Ik) in pyramidal neurons in cortex and hippocampus. The neuronal protective effect of propofol has been proved. Delayed rectifier potassium current (Ik) has been shown to have close relationship with neuronal damage. The study was designed to test the inhibitory effect of propofol on Kv2.1 potassium channel in rat parietal cortical neurons. Whole-cell patch clamp recordings and Western blot analysis were used to investigate the electrophysiological function and protein expression of Kv2.1 in rat parietal cortical neurons after propofol treatment. We found that propofol concentration-dependently inhibited Ik in pyramidal neurons. Propofol also caused a downward shift of the I-V curve of Ik at 30μM concentration. Propofol significantly inhibited the expression of Kv2.1 protein level at 30μM, 50μM, 100μM concentration. In conclusion, our data showed that propofol could inhibit Ik, probably via depressing the expression of Kv2.1 protein in rat cerebral parietal cortical neurons.
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Yan-Zhuo
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Xian-Zhang
authors.initials: XZ
authors.lastname: Zeng
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. Electronic address: scy-zw@163.com.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Song
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D005260:Female; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010296:Parietal Lobe / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015742:Propofol / Q000494:pharmacology*; D017208:Rats, Wistar; D051669:Shab Potassium Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D018686:Anesthetics, Intravenous; D018696:Neuroprotective Agents; D051669:Shab Potassium Channels; D015742:Propofol
keywords: Delayed rectifier potassium current; Kv2.1 potassium channel; Parietal cortical neuron; Propofol; Whole-cell patch clamp