title: Expression of CD11c+HLA-DR+dendritic cells and related cytokines in the follicular fluid might be related to pathogenesis of ovarian hyperstimulation syndrome.
abstract: OBJECTIVE
  To explore the expressions of CD11c+HLA-DR+dentritic cells in the follicular fluid of patients with OHSS and their significances.
  
  
  METHODS
  100 individuals.
  
  
  METHODS
  embryos were observed. The distribution of dentritic cells in follicular fluid and the levels of IL-10, IL-12, IL-18 and IL-23 in follicular fluid were detected.
  
  
  METHODS
  There were ovarian hyperstimulation syndrome (OHSS) group and control group in this study. The OHSS group consisted of 50 patients with OHSS and the control group consisted of 50 patients who underwent in vitro fertilization-embryo transfer (IVF-ET) only due to male factors. The statuses of embryos were compared between the two groups. The distribution of dentritic cells in follicular fluid was determined with flow cytometry, and the levels of IL-10, IL-12, IL-18 and IL-23 in follicular fluid were detected with enzyme-linked immunosorbent assay (ELISA) in all patients.
  
  
  RESULTS
  The two-pronuclear (2PN) fertility rate, high-quality embryo rate and available embryo rate were all significantly lower in OHSS group than in control group (all P<0.05). The number of CD11c+HLA-DR+dentritic cells (P<0.05) and the levels of IL-10, IL-12, IL-18 and IL-23 were all significantly higher in OHSS group than in control group (all P<0.01).
  
  
  CONCLUSIONS
  The follicular fluid of the patients with OHSS is in an inflammatory status, the inflammatory status may be involved in OHSS and the microenvironment of follicular fluid may affects oocyte quality and embryo development.
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Sen-Lin
authors.initials: SL
authors.lastname: Shi
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Zhao-Feng
authors.initials: ZF
authors.lastname: Peng
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Gui-Dong
authors.initials: GD
authors.lastname: Yao
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Jin
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Song
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Yang
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Ru-Yue
authors.initials: RY
authors.lastname: Xue
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450001, China.
authors.forename: Ying-Pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D039521:CD11c Antigen / Q000378:metabolism; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism*; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects; D005434:Flow Cytometry; D015571:Follicular Fluid / Q000737:chemistry* / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D016471:Ovarian Hyperstimulation Syndrome / Q000378:metabolism*; D010062:Ovulation Induction / Q000009:adverse effects
chemical_list: D039521:CD11c Antigen; D016207:Cytokines; D006684:HLA-DR Antigens
keywords: Ovarian hyperstimulation syndrome; cytokines; dentritic cells; inflammatory reaction
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