title: Acutifoliside, a novel benzoic acid glycoside from Salix acutifolia.
abstract: Ultra high-performance liquid chromatography-mass spectrometry (UHPLC-MS) profiling of a polar solvent extract of juvenile stem tissue of Salix acutifolia Willd. identified a range of phenolic metabolites. Salicortin, 1, a well-known salicinoid, was the major compound present and the study identified young stem tissue of this species as a potential source of this compound for future studies. Three further known metabolites (salicin 2, catechin 3 and tremuloidin 4) were also present. The UHPLC-MS analysis also revealed the presence of a further, less polar, unknown compound, which was isolated via HPLC peak collection. The structure was elucidated by high-resolution mass spectroscopic analysis, 1- and 2-D NMR analysis and chemical derivatisation and was shown to be a novel benzoic acid glycoside 5, which we have named as acutifoliside.
authors.affiliation: a Department of Plant Biology and Crop Science , Rothamsted Research , Harpenden , UK.
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: a Department of Plant Biology and Crop Science , Rothamsted Research , Harpenden , UK.
authors.forename: Darja
authors.initials: D
authors.lastname: Dobermann
authors.affiliation: a Department of Plant Biology and Crop Science , Rothamsted Research , Harpenden , UK.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Beale
authors.affiliation: a Department of Plant Biology and Crop Science , Rothamsted Research , Harpenden , UK.
authors.forename: Jane L
authors.initials: JL
authors.lastname: Ward
mesh_terms: D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification; D001592:Benzyl Alcohols / Q000737:chemistry / Q000302:isolation & purification; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012459:Salicylates / Q000737:chemistry / Q000302:isolation & purification*; D032108:Salix / Q000737:chemistry*
chemical_list: D001565:Benzoates; D001592:Benzyl Alcohols; D005960:Glucosides; D006027:Glycosides; D012459:Salicylates; C005696:salicin; D002392:Catechin; C113068:salicortin
keywords: NMR; Salix acutifolia; UHPLC–MS; acutifoliside; benzoic acid glycoside; phenolic glycoside