title: Neurotrophic activity of jiadifenolide on neuronal precursor cells derived from human induced pluripotent stem cells.
abstract: Although jiadifenolide has been reported to neurotrophin-like activity in primary cultured rat cortical neurons, it is unknown on that of activity in human neurons. Thus, we aimed to assess neurotrophin-like activity by jiadifenolide in human neuronal cells. We analyzed neuronal precursor cells derived from human induced pluripotent stem cells for microtuble-associated-protein-2 expression by immunofluorescence and western blot, following jiadifenolide treatment. Jiadifenolide promoted dendrite outgrowth, facilitated growth, and prevented death in neuronal cells derived from human induced pluripotent stem cells. Interestingly, jiadifenolide also increased postsynaptic density-95 protein expression suggesting that jiadifenolide promotes neuronal maturation and post-synaptic formation. We demonstrate for the first time that jiadifenolide exhibits neurotrophic effects on human neuronal precursor cells.
authors.affiliation: Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Shoji
authors.affiliation: Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Nishioka
authors.affiliation: Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Minato
authors.affiliation: Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Harada
authors.affiliation: Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Kubo
authors.affiliation: Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Fukuyama
authors.affiliation: Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan. Electronic address: kuzuhara@ph.bunri-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuzuhara
mesh_terms: D002454:Cell Differentiation; D049108:Cell Enlargement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009414:Nerve Growth Factors / Q000008:administration & dosage*; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D012717:Sesquiterpenes / Q000008:administration & dosage*
chemical_list: D009414:Nerve Growth Factors; D012717:Sesquiterpenes; C546282:jiadifenolide
keywords: Jiadifenolide; MAP2; Neurite outgrowth; Neurotrophin; PSD95; hiPSC