title: Effect of Bisphenol A on invasion ability of human trophoblastic cell line BeWo.
abstract: Bisphenol A (BPA) is a kind of environmental endocrine disruptors (EEDs) that interfere embryo implantation. Trophoblast invasion plays a crucial role during embryo implantation. In this study, the effects of BPA on invasion ability of human trophoblastic cell line BeWo and its possible mechanism were investigated. BeWo cells were exposed to BPA and co-cultured with human endometrial cells to mimic embryo implantation in transwell model. The proliferation and invasion capability of BeWo cells were detected. The expression of E-cadherin, DNMT1, MMP-2, MMP-9, TIMP-1 and TIMP-2 were also analyzed. The results showed that the invasion capability of BeWo was reduced after daily exposure to BPA. BPA had biphasic effect on E-cadherin expression level in BeWo cells and expression level of DNMT1 was decreased when treated with BPA. Moreover, BPA treatment also changed the balance of MMPs/TIMPs in BeWo cells by down-regulating MMP-2, MMP-9 and up-regulating TIMP-1, TIMP-2 with increasing BPA concentration. Taken together, these results showed that BPA treatment could reduce the invasion ability of BeWo cells and alter the expression level of E-cadherin, DNMT1, TIMP-1, TIMP-2, MMP-2, and MMP-9. Our study would help us to understand the possible mechanism of BPA effect on invasion ability of human trophoblastic cell line BeWo.
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China; Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China.
authors.forename: Zi-Yi
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China; Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China; Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China.
authors.forename: Yuan-Zhen
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China; Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China; Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China.
authors.forename: Teng
authors.initials: T
authors.lastname: Liu
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China; Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityWuhan 430071, Hubei Province, People's Republic of China.
authors.forename: Xin-Lan
authors.initials: XL
authors.lastname: Qu
mesh_terms: D015703:Antigens, CD; D001559:Benzhydryl Compounds / Q000633:toxicity*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000098826:Bisphenol A Compounds
chemical_list: D015703:Antigens, CD; D001559:Benzhydryl Compounds; D015820:Cadherins; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D052244:Endocrine Disruptors; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D010636:Phenols; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; C513384:CDH1 protein, human; C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; C000619380:DNMT1 protein, human; C522361:MMP2 protein, human; C579270:MMP9 protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: BeWo cells; Bisphenol A; DNMT1; E-cadherin; MMPs/TIMPs; cell invasion
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