title: The cancer-retina antigen recoverin as a potential biomarker for renal tumors.
abstract: The renal cell carcinoma is the ninth most common cancer with an increasing occurrence and mortality. Recoverin is the first retina-specific photoreceptor protein that was shown to undergo aberrant expression, due to its promoter demethylation, as a cancer-retina antigen in a number of malignant tumors. In this work, we demonstrated that recoverin is indeed expressed in 68.4 % of patients with different subtypes of renal cell carcinoma, and this expression has tendency to correlate with tumor size. Interestingly, 91.7 % of patients with the benign renal tumor, oncocytoma, express recoverin as well in their tumor. Epigenetic analysis of the recoverin gene promoter revealed a stable mosaic methylation pattern with the predominance of the methylated state, with the exception of -80 and 56 CpG dinucleotides (CpGs). While the recoverin expression does not correlate withoverall survival of the tumor patients, the methylation of the recoverin gene promoter at -80 position is associated with better overall survival of the patients. This work is the first report pointing towards the association of overall survival of renal cell carcinoma (RCC) patients with promoter methylation of a cancer-retina antigen. Taken together, these data allow to consider recoverin as a potential therapeutic target and/or marker for renal tumors.
authors.affiliation: Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia.
authors.forename: Marina O
authors.initials: MO
authors.lastname: Golovastova
authors.affiliation: Anatomic Pathology Department, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Larisa V
authors.initials: LV
authors.lastname: Tsoy
authors.affiliation: Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Bocharnikova
authors.affiliation: Institute of Uronephrology and Human Reproductive Health, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Dmitry O
authors.initials: DO
authors.lastname: Korolev
authors.affiliation: Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Gancharova
authors.affiliation: Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.|Research Centre for Medical Genetics, 115472, Moscow, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Alekseeva
authors.affiliation: Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.|Research Centre for Medical Genetics, 115472, Moscow, Russia.
authors.forename: Ekaterina B
authors.initials: EB
authors.lastname: Kuznetsova
authors.affiliation: Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Lyudmila V
authors.initials: LV
authors.lastname: Savvateeva
authors.affiliation: Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia.
authors.forename: Elena E
authors.initials: EE
authors.lastname: Skorikova
authors.affiliation: Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.|Research Centre for Medical Genetics, 115472, Moscow, Russia.|Medical Biology Faculty, Department of Molecular Genetics and Cytogenetics, Pirogov Russian National Research Medical University, 117997, Moscow, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Strelnikov
authors.affiliation: Anatomic Pathology Department, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Varshavsky
authors.affiliation: Institute of Uronephrology and Human Reproductive Health, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Andrey Z
authors.initials: AZ
authors.lastname: Vinarov
authors.affiliation: Sechenov First Moscow State Medical University, 119991, Moscow, Russia.|Faculty of Basic Medicine, Lomonosov Moscow State University, 119991, Moscow, Russia.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Nikolenko
authors.affiliation: Institute of Uronephrology and Human Reproductive Health, Sechenov First Moscow State Medical University, 119991, Moscow, Russia.
authors.forename: Peter V
authors.initials: PV
authors.lastname: Glybochko
authors.affiliation: Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia.
authors.forename: Evgeni Yu
authors.initials: EY
authors.lastname: Zernii
authors.affiliation: Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia. zamyat@belozersky.msu.ru.|Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia. zamyat@belozersky.msu.ru.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Zamyatnin
authors.affiliation: Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, 81377, Munich, Germany. alexandr.bazhin@med.uni-muenchen.de.
authors.forename: Alexandr V
authors.initials: AV
authors.lastname: Bazhin
authors.affiliation: Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia.
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Philippov
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002291:Carcinoma, Papillary / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019175:DNA Methylation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D051603:Recoverin / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D051603:Recoverin
keywords: Cancer-retina antigens; DNA methylation; Onconeural antigens; Recoverin; Renal cancer
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