title: Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells.
abstract: The administration of human adipose-derived stem cells (ASCs) represents a promising regenerative therapy for the treatment of orthopedic injuries. While ASCs can be easily isolated from liposuction-derived adipose tissue, most clinical applications will likely require in vitro culture expansion of these cells using nonxenogeneic components. In this study, platelet releasate was generated using a novel rapid thrombin activation method (tPR). ASCs grown in media supplemented with tPR proliferated much faster than ASCs grown in media supplemented with 10% fetal bovine serum. The cells also retained the ability to differentiate along chondrogenic, adipogenic, and osteogenic lineages. The tPR cultured ASCs displayed elevated expression of BMP-4 (5.7 ± 0.97-fold increase) and BMP-2 (4.7 ± 1.3-fold increase) and decreased expression of PDGF-B (4.0 ± 1.4-fold decrease) and FGF-2 (33 ± 9.0-fold decrease). No significant changes in expression were seen with TGF-β and VEGF. This pattern of gene expression was consistent across different allogeneic tPR samples and different ASC lines. The use of allogeneic rapidly activated tPR to culture ASCs is associated with both an increased cell yield and a defined gene expression profile making it an attractive option for cell expansion prior to cell-based therapy for orthopedic applications.
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: McLaughlin
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Gagnet
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Cunningham
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Randi
authors.initials: R
authors.lastname: Yeager
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: D'Amico
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Katie
authors.initials: K
authors.lastname: Guski
authors.affiliation: Trinity Sports Medicine and Performance Center, Trinity Hospital, Steubenville, OH 43952, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Scarpone
authors.affiliation: Department of Biology, Franciscan University of Steubenville, Steubenville, OH 43952, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kuebler
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