title: PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis.
abstract: Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 peaks at dusk, binds phytochrome B (phyB) in a red light-dependent manner, and co-localizes with phyB into photobodies. PCH1 is necessary and sufficient to promote the biogenesis of large photobodies to maintain an active phyB pool after light exposure, potentiating red-light signaling and prolonging memory of prior illumination. Manipulating PCH1 alters PHYTOCHROME INTERACTING FACTOR 4 levels and regulates light-responsive gene expression. Thus, PCH1 is a new factor that regulates photoperiod-responsive growth by integrating the clock with light perception pathways through modulating daily phyB-signaling.
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, United States.
authors.forename: He
authors.identifier: http://orcid.org/0000-0002-4564-7604
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, United States.
authors.forename: Chan Yul
authors.identifier: http://orcid.org/0000-0001-6159-7443
authors.initials: CY
authors.lastname: Yoo
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, United States.
authors.forename: Rebecca
authors.identifier: http://orcid.org/0000-0001-7994-2073
authors.initials: R
authors.lastname: Bindbeutel
authors.affiliation: Michigan State University, East Lansing, United States.
authors.forename: Jessica
authors.initials: J
authors.lastname: Goldsworthy
authors.affiliation: Mary Institute and Saint Louis Country Day School, St. Louis, United States.
authors.forename: Allison
authors.initials: A
authors.lastname: Tielking
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, United States.
authors.forename: Sophie
authors.identifier: http://orcid.org/0000-0001-8550-2832
authors.initials: S
authors.lastname: Alvarez
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, United States.
authors.forename: Michael J
authors.identifier: http://orcid.org/0000-0002-6899-5652
authors.initials: MJ
authors.lastname: Naldrett
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, United States.
authors.forename: Bradley S
authors.identifier: http://orcid.org/0000-0002-1207-9006
authors.initials: BS
authors.lastname: Evans
authors.affiliation: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, United States.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, United States.
authors.forename: Dmitri A
authors.identifier: http://orcid.org/0000-0002-0497-1723
authors.initials: DA
authors.lastname: Nusinow
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D057906:Circadian Clocks; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D008027:Light; D056511:Metallochaperones / Q000235:genetics / Q000378:metabolism*; D017440:Photoperiod; D051939:Phytochrome B / Q000378:metabolism; D063245:Plant Development; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D056511:Metallochaperones; C000597337:PCH1 protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D051939:Phytochrome B
keywords: <i>a. thaliana</i>; circadian rhythm; photobody; photoperiodism; phytochrome; plant biology; plant growth; signal transduction
references.citation: Development. 1999 Oct;126(19):4235-45
references.pmid: 10477292
references.citation: Mol Cell. 2006 Aug 4;23(3):439-46
references.pmid: 16885032
references.citation: Plant Cell. 2004 Jun;16(6):1433-45
references.pmid: 15155879
references.citation: Plant Cell. 2010 Jul;22(7):2370-83
references.pmid: 20605855
references.citation: Anal Chem. 2002 Oct 15;74(20):5383-92
references.pmid: 12403597
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: Curr Biol. 2005 Dec 6;15(23):2125-30
references.pmid: 16332538
references.citation: Trends Plant Sci. 2014 Apr;19(4):240-9
references.pmid: 24373845
references.citation: Plant Mol Biol. 1994 Jun;25(3):413-27
references.pmid: 8049367
references.citation: Plant Cell. 2015 May;27(5):1409-27
references.pmid: 25944101
references.citation: Plant Cell. 2001 Jun;13(6):1281-92
references.pmid: 11402160
references.citation: Nature. 2002 Sep 5;419(6902):74-7
references.pmid: 12214234
references.citation: Gene. 1993 Jul 30;129(2):175-82
references.pmid: 8325504
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Curr Top Dev Biol. 2010;91:29-66
references.pmid: 20705178
references.citation: Nucleic Acids Res. 2012 Jan;40(Database issue):D1202-10
references.pmid: 22140109
references.citation: Biochemistry. 2015 Jan 20;54(2):157-70
references.pmid: 25346271
references.citation: PLoS Genet. 2009 Jan;5(1):e1000352
references.pmid: 19165330
references.citation: PLoS Biol. 2008 Sep 16;6(9):e225
references.pmid: 18798691
references.citation: Trends Cell Biol. 2011 Nov;21(11):664-71
references.pmid: 21852137
references.citation: Genes Dev. 1989 Nov;3(11):1745-57
references.pmid: 2606345
references.citation: Science. 2004 Feb 13;303(5660):1003-6
references.pmid: 14963328
references.citation: Plant Cell. 2004 Nov;16(11):3033-44
references.pmid: 15486100
references.citation: Plant Cell. 2009 Mar;21(3):786-99
references.pmid: 19286967
references.citation: Dev Cell. 2015 Nov 9;35(3):311-21
references.pmid: 26555051
references.citation: Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):761-6
references.pmid: 23267111
references.citation: Plant J. 2007 Aug;51(4):551-62
references.pmid: 17617174
references.citation: Cell Rep. 2013 Jun 27;3(6):1970-9
references.pmid: 23746445
references.citation: Genes Dev. 2003 Nov 1;17(21):2642-7
references.pmid: 14597662
references.citation: Science. 2009 Mar 13;323(5920):1481-5
references.pmid: 19286557
references.citation: Cell. 2010 Jun 25;141(7):1230-40
references.pmid: 20603003
references.citation: Mol Cell Proteomics. 2016 Jan;15(1):201-17
references.pmid: 26545401
references.citation: Cold Spring Harb Symp Quant Biol. 2007;72:353-63
references.pmid: 18419293
references.citation: Nature. 2011 Jul 21;475(7356):398-402
references.pmid: 21753751
references.citation: Nat Methods. 2013 Aug;10(8):730-6
references.pmid: 23921808
references.citation: Development. 2012 Nov;139(22):4180-90
references.pmid: 23034631
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10387-92
references.pmid: 16006522
references.citation: Plant Cell. 2002 Jul;14(7):1541-55
references.pmid: 12119373
references.citation: PLoS Genet. 2008 Feb;4(2):e14
references.pmid: 18248097
references.citation: Annu Rev Plant Biol. 2006;57:837-58
references.pmid: 16669784
references.citation: Mol Cell. 2008 Dec 5;32(5):617-30
references.pmid: 19061637
references.citation: Curr Biol. 2012 Aug 21;22(16):R648-57
references.pmid: 22917516
references.citation: Trends Plant Sci. 2002 May;7(5):193-5
references.pmid: 11992820
references.citation: Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10179-84
references.pmid: 24982198
references.citation: Nat Plants. 2015 Jul 06;1:15090
references.pmid: 27250256
references.citation: Plant Physiol. 2012 May;159(1):366-90
references.pmid: 22383539
references.citation: Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):15054-9
references.pmid: 22927419
references.citation: Plant Cell. 2001 Jun;13(6):1293-304
references.pmid: 11402161
references.citation: Genes Dev. 2004 May 15;18(10):1179-86
references.pmid: 15131083
references.citation: Plant J. 2009 Nov;60(3):449-61
references.pmid: 19619162
references.citation: Nature. 2008 Jan 24;451(7177):480-4
references.pmid: 18216857
references.citation: Plant Cell Physiol. 2012 Nov;53(11):1950-64
references.pmid: 23037003
references.citation: PLoS One. 2010;5(5):e10721
references.pmid: 20502669
references.citation: Plant Cell. 2014 Oct;26(10):3829-37
references.pmid: 25361954
references.citation: Science. 2006 Sep 15;313(5793):1596-604
references.pmid: 16973872
references.citation: Plant Cell Physiol. 2009 Apr;50(4):838-54
references.pmid: 19233867
references.citation: Plant Cell. 1993 Feb;5(2):147-57
references.pmid: 8453299
references.citation: Plant Signal Behav. 2013 Mar;8(3):e23390
references.pmid: 23299336
references.citation: Plant J. 2008 Jan;53(2):312-23
references.pmid: 18047474
references.citation: Nature. 2010 Feb 11;463(7282):763-8
references.pmid: 20148030
references.citation: EMBO J. 2002 May 15;21(10):2441-50
references.pmid: 12006496
references.citation: Nature. 2005 Jul 14;436(7048):282-4
references.pmid: 16015335
references.citation: Nat Biotechnol. 2014 Mar;32(3):223-6
references.pmid: 24727771
references.citation: Plant J. 2012 Aug;71(3):390-401
references.pmid: 22409654
references.citation: Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14493-8
references.pmid: 14612575
references.citation: Philos Trans R Soc Lond B Biol Sci. 2010 Oct 12;365(1555):3129-47
references.pmid: 20819808
references.citation: Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8487-92
references.pmid: 26100864
references.citation: Trends Plant Sci. 2008 Mar;13(3):137-44
references.pmid: 18314376
references.citation: Curr Biol. 2005 Apr 12;15(7):637-42
references.pmid: 15823535
references.citation: Mol Syst Biol. 2012;8:574
references.pmid: 22395476
references.citation: Methods Mol Biol. 2014;1158:123-37
references.pmid: 24792048
references.citation: Anal Chem. 2003 Sep 1;75(17):4646-58
references.pmid: 14632076
references.citation: Plant Cell Physiol. 2011 Aug;52(8):1315-29
references.pmid: 21666227
references.citation: Nature. 2007 Jul 19;448(7151):358-61
references.pmid: 17589502
references.citation: Science. 2005 Jul 22;309(5734):630-3
references.pmid: 16040710
references.citation: Nucleic Acids Res. 2007 Jan;35(Database issue):D883-7
references.pmid: 17145706
references.citation: Genes Cells. 2005 Oct;10(10):963-72
references.pmid: 16164597
references.citation: Plant J. 2007 Mar;49(6):1064-75
references.pmid: 17335514
references.citation: Genome Biol. 2007;8(2):R19
references.pmid: 17291332
references.citation: J Cell Biol. 1999 May 3;145(3):437-45
references.pmid: 10225946
references.citation: Plant Physiol. 2014 Apr 25;165(2):595-607
references.pmid: 24769533
references.citation: Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10171-6
references.pmid: 19520826
references.citation: Curr Biol. 2012 Aug 21;22(16):1530-5
references.pmid: 22818915
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):17193-8
references.pmid: 18971337