title: KIR2DL4 expression rather than its single nucleotide polymorphisms correlates with pre-eclampsia.
abstract: OBJECTIVE
  To evaluate the single nucleotide polymorphisms and expression of KIR2DL4 (killer cell immunoglobulin-like receptors) gene in pre-eclampsia patients.
  
  
  METHODS
  KIR2DL4 gene polymorphisms were detected in 100 patients with pre-eclampsia and 100 healthy pregnant women, respectively, by using PCR-SS. Then, the expression of KIR2DL4 was measured in 5 cases of placentas tissues with pre-eclampsia and normal pregnancies by using qRT-PCR.
  
  
  RESULTS
  Compared with healthy controls, 16 loci of single nucleotide polymorphisms (SNP) were identified in pre-eclampsia patients, including 7 new polymorphisms loci. But, no significant difference was found in genotype distributions and allele frequencies in pre-eclampsia and controls (P>0.05). However, qRT-PCR results showed that KIR2DL4 mRNA in placenta tissues with pre-eclampsia was significantly lower than those with normal pregnancy, and the difference was statistically significant.
  
  
  CONCLUSIONS
  Decreased level of KIR2DL4 rather than its SNP is correlated with the susceptibility of pre-eclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Lianlian
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Xianying
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Fuju
authors.initials: F
authors.lastname: Wu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D054345:Receptors, KIR2DL4 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C515079:KIR2DL4 protein, human; D054345:Receptors, KIR2DL4
keywords: KIR2DL4; pre-eclampsia; single nucleotide polymorphisms; susceptibility
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