title: Decreased Histone Deacetylase 2 (HDAC2) in Peripheral Blood Monocytes (PBMCs) of COPD Patients.
abstract: BACKGROUND
  Histone deacetylase 2 (HDAC2) is a class I histone deacetylase family member that plays a critical role in suppressing inflammatory gene expression in the airways, lung parenchyma, and alveolar macrophages in patients with chronic obstructive pulmonary disease (COPD). However, the expression of HDAC2 in peripheral blood monocytes (PBMCs), nuclear factor kappa B (NF-κB) p65, and serum inflammatory cytokine levels in COPD patients, smokers, and non-smokers remains unclear.
  
  
  METHODS
  PBMCs were obtained from COPD patients, healthy smokers, and healthy nonsmokers. The HDAC2 and NF-κB p65 expression were quantified by Western Blot. HDAC activity was assessed by an HDAC fluorometric immunoprecipitation activity assay kit. Serum tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) levels were measured by ELISA.
  
  
  RESULTS
  HDAC2 expression and HDAC activity were decreased in PBMCs in COPD patients compared with smokers and non-smokers. Increased NF-κB p65 expression, serum TNF-α and IL-8 levels were observed in COPD patients compared with nonsmokers. The FEV1%pred was positively correlated with HDAC2 expression and HDAC activity in COPD patients. Smokers had decreased HDAC activity, increased NF-κB p65 expression and serum TNF-α compared with nonsmokers.
  
  
  CONCLUSIONS
  HDAC2 expression was decreased in PBMCs of COPD patients and was correlated with disease severity. The reduction of HDAC2 expression not only directly enhances the expression of inflammatory genes, but may account for the activation of NF-κB mediated inflammation. Decreased HDAC2 may serve as a potential biomarker of COPD and predict the decline of lung function.
authors.affiliation: Department of Respiratory Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
authors.forename: Chunting
authors.initials: C
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.|State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Xuan
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Respiratory Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
authors.forename: Ganggang
authors.initials: G
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Hou
authors.affiliation: Department of Respiratory Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
authors.forename: Haoyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000368:Aged; D016207:Cytokines / Q000097:blood; D005260:Female; D056464:Histone Deacetylase 2 / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000201:enzymology*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D016207:Cytokines; D051996:Transcription Factor RelA; D056464:Histone Deacetylase 2
references.citation: Mol Biol Rep. 2013 Jun;40(6):3995-4000
references.pmid: 23275209
references.citation: J Biol Chem. 2014 Mar 7;289(10):6383-93
references.pmid: 24368768
references.citation: Eur Respir J. 2002 Sep;20(3):556-63
references.pmid: 12358328
references.citation: J Exp Med. 2006 Jan 23;203(1):7-13
references.pmid: 16380507
references.citation: FASEB J. 2001 Apr;15(6):1110-2
references.pmid: 11292684
references.citation: Am J Physiol Lung Cell Mol Physiol. 2006 Jul;291(1):L46-57
references.pmid: 16473865
references.citation: Biochem Pharmacol. 2002 Sep;64(5-6):935-42
references.pmid: 12213589
references.citation: Mol Cell Biol. 2001 Oct;21(20):7065-77
references.pmid: 11564889
references.citation: J Exp Med. 2004 Sep 6;200(5):689-95
references.pmid: 15337792
references.citation: COPD. 2009 Aug;6(4):291-7
references.pmid: 19811389
references.citation: Antioxid Redox Signal. 2008 Apr;10(4):799-811
references.pmid: 18220485
references.citation: Am J Physiol Lung Cell Mol Physiol. 2012 Oct 1;303(7):L557-66
references.pmid: 22842217
references.citation: Annu Rev Physiol. 2009;71:451-64
references.pmid: 18817512
references.citation: N Engl J Med. 2005 May 12;352(19):1967-76
references.pmid: 15888697
references.citation: Clin Chem Lab Med. 2015 Jan;53(1):57-64
references.pmid: 25029083
references.citation: Proc Am Thorac Soc. 2006 Nov;3(8):668-72
references.pmid: 17065371
references.citation: Am J Pathol. 2012 Feb;180(2):562-74
references.pmid: 22265050
references.citation: Eur Respir J. 2012 Oct;40(4):863-73
references.pmid: 22441735
references.citation: J Inflamm (Lond). 2012 Mar 23;9:10
references.pmid: 22443498
references.citation: Implant Dent. 2013 Oct;22(5):519-24
references.pmid: 23823734
references.citation: Niger J Clin Pract. 2013 Jan-Mar;16(1):76-81
references.pmid: 23377476
references.citation: Am J Respir Cell Mol Biol. 2009 Apr;40(4):464-73
references.pmid: 18927347
references.citation: PLoS One. 2012;7(8):e42152
references.pmid: 22879913
references.citation: Am J Respir Crit Care Med. 2006 Jul 1;174(1):41-50
references.pmid: 16574938
references.citation: Ther Adv Respir Dis. 2009 Oct;3(5):235-43
references.pmid: 19812111
references.citation: Drugs. 2010 May 28;70(8):929-48
references.pmid: 20481652
references.citation: COPD. 2005 Dec;2(4):445-55
references.pmid: 17147010
references.citation: Am J Respir Crit Care Med. 2006 Jul 1;174(1):2-3
references.pmid: 16793997
references.citation: Eur Respir J. 2005 Mar;25(3):552-63
references.pmid: 15738302
references.citation: Biomarkers. 2009 Jul;14 Suppl 1:90-6
references.pmid: 19604067
references.citation: Expert Opin Ther Targets. 2007 Jun;11(6):745-55
references.pmid: 17504013
references.citation: Biomolecules. 2015;5(3):1266-83
references.pmid: 26131974
references.citation: Arch Bronconeumol. 2011 Apr;47(4):176-83
references.pmid: 21454005