title: Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae.
abstract: Structural and functional analysis of proteins involved in pre-mRNA splicing is challenging because of the complexity of the splicing machinery, known as the spliceosome. Bioinformatic, proteomic, and biochemical analyses have identified a minimal spliceosome in the red alga Cyanidioschyzon merolae. This spliceosome consists of only 40 core proteins, compared to ∼ 70 in S. cerevisiae (yeast) and ∼ 150 in humans. We report the X-ray crystallographic analysis of C. merolae Snu13 (CmSnu13), a key component of the assembling spliceosome, and present evidence for conservation of Snu13 function in this algal splicing pathway. The near identity of CmSnu13's three-dimensional structure to yeast and human Snu13 suggests that C. merolae should be an excellent model system for investigating the structure and function of the conserved core of the spliceosome.
authors.affiliation: Department of Chemistry, University of Northern British Columbia, Prince George, British Columbia, V2N 4Z9, Canada.
authors.forename: C S
authors.initials: CS
authors.lastname: Black
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
authors.forename: E L
authors.initials: EL
authors.lastname: Garside
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
authors.forename: A M
authors.initials: AM
authors.lastname: MacMillan
authors.affiliation: Department of Chemistry, University of Northern British Columbia, Prince George, British Columbia, V2N 4Z9, Canada.
authors.forename: S D
authors.identifier: http://orcid.org/0000-0001-7242-1785
authors.initials: SD
authors.lastname: Rader
mesh_terms: D020418:Algal Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D012326:RNA Splicing; D000461:Rhodophyta / Q000737:chemistry / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017381:Spliceosomes / Q000235:genetics / Q000378:metabolism*
chemical_list: D020418:Algal Proteins; D017411:Ribonucleoproteins, Small Nuclear; C402291:Snu13 protein, human
keywords: Cyanidioschyzon merolae; RNA binding; U4 snRNP; X-ray crystallography; fluorescence polarization; minimal spliceosome; pre-mRNA splicing; snRNP assembly; thermophile
references.citation: RNA. 1999 Feb;5(2):221-34
references.pmid: 10024174
references.citation: Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7226-31
references.pmid: 10377396
references.citation: EMBO J. 1999 Nov 1;18(21):6119-33
references.pmid: 10545122
references.citation: J Mol Biol. 2000 Sep 8;302(1):205-17
references.pmid: 10964570
references.citation: Cell. 2000 Oct 27;103(3):457-66
references.pmid: 11081632
references.citation: Mol Cell. 2000 Dec;6(6):1331-42
references.pmid: 11163207
references.citation: Protein Eng. 2001 Dec;14(12):993-1000
references.pmid: 11809930
references.citation: Nucleic Acids Res. 2002 Feb 15;30(4):931-41
references.pmid: 11842104
references.citation: J Mol Biol. 2002 May 3;318(3):707-21
references.pmid: 12054817
references.citation: EMBO J. 2002 Oct 15;21(20):5527-38
references.pmid: 12374753
references.citation: Chem Biol. 2002 Oct;9(10):1095-107
references.pmid: 12401494
references.citation: Mol Cell Biol. 2002 Dec;22(23):8342-52
references.pmid: 12417735
references.citation: RNA. 2003 Jul;9(7):821-38
references.pmid: 12810916
references.citation: Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1131-7
references.pmid: 12832755
references.citation: RNA. 2004 Feb;10(2):254-64
references.pmid: 14730024
references.citation: Nature. 2004 Apr 8;428(6983):653-7
references.pmid: 15071595
references.citation: Structure. 2004 May;12(5):893-903
references.pmid: 15130481
references.citation: Biochem Biophys Res Commun. 2005 Jul 29;333(2):550-4
references.pmid: 15963469
references.citation: Appl Environ Microbiol. 2005 Nov;71(11):7164-71
references.pmid: 16269755
references.citation: Biochem Biophys Res Commun. 2007 Sep 7;360(4):857-62
references.pmid: 17631273
references.citation: Biochemistry. 2007 Dec 25;46(51):14979-86
references.pmid: 18044964
references.citation: Annu Rev Cell Biol. 1991;7:559-99
references.pmid: 1839712
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
references.pmid: 20124702
references.citation: RNA. 2011 Sep;17(9):1655-63
references.pmid: 21784869
references.citation: Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):E1191-200
references.pmid: 25733880
references.citation: Nature. 2015 Jul 2;523(7558):47-52
references.pmid: 26106855
references.citation: Elife. 2015 Jul 10;4:e07320
references.pmid: 26161500
references.citation: Nucleic Acids Res. 2015 Dec 15;43(22):10963-74
references.pmid: 26503251
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Annu Rev Biochem. 1995;64:897-934
references.pmid: 7574504