title: Protective effect of a polysaccharide from Rhizoma Atractylodis Macrocephalae on acute liver injury in mice.
abstract: A homogeneous polysaccharide was isolated and purified from Rhizoma Atractylodis Macrocephalae (RAM) and named PRAM2. Its average molecular weight was 19.6×10(3)Da and it was composed of rhamnose, xylose, arabinose, glucose, mannose and galactose in a ratio of 1: 1.3: 1.5: 1.8: 2.1: 3.2. In vitro experiments confirmed that PRAM2 presented an obvious effect to scavenge 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) (DPPH), superoxide anion and hydroxyl radical. In vivo experiments confirmed that PRAM2 could reduce the liver weight, liver index, aspartate transaminase (AST) and alanine aminotransferase (ALT) activities in the serum; meanwhile, PRAM2 could significantly reduce nitric oxide synthase (NOS) activity, and nitric oxide (NO) and malonaldehyde (MDA) contents in the liver tissues, and increase superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. These results suggest that PRAM2 has a significant in vitro antioxidant activity and a protective effect on CCl4-induced liver injury in mice; the protective effect may be related to its anti-oxidation, its inhibition of NOS activity and NO level and its reduction of the production of free radicals.
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Beijing Stomatological Hospital, The Capital Medical University, Beijing 100050, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Changchun Women and Children Health Hospital, Changchun 130051, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Zuhui
authors.initials: Z
authors.lastname: Shang
authors.affiliation: China Japan Union Hospital, Jilin University, Changchun 130033, China. Electronic address: ccwangshuang@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. Electronic address: peijin@jlu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Pei
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031174:Atractylodes / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000009:adverse effects; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009569:Nitric Oxide / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D009569:Nitric Oxide; D008315:Malondialdehyde; D002251:Carbon Tetrachloride
keywords: Liver injury; Polysaccharide; Rhizoma Atractylodis Macrocephalae