title: Additive enhancement of wound healing in diabetic mice by low level light and topical CoQ10.
abstract: Diabetes, a highly prevalent disease that affects 9.3% of Americans, often leads to severe complications and slow wound healing. Preclinical studies have suggested that low level light therapy (LLLT) can accelerate wound healing in diabetic subjects, but significant improvements must be made to overcome the absence of persuasive evidence for its clinical use. We demonstrate here that LLLT can be combined with topical Coenzyme Q10 (CoQ10) to heal wounds in diabetic mice significantly faster than LLLT alone, CoQ10 alone, or controls. LLLT followed by topical CoQ10 enhanced wound healing by 68~103% in diabetic mice in the first week and more than 24% in the second week compared with untreated controls. All wounds were fully healed in two weeks following the dual treatment, in contrast to only 50% wounds or a fewer being fully healed for single or sham treatment. The accelerated healing was corroborated by at least 50% higher hydroxyproline levels, and tripling cell proliferation rates in LLLT and CoQ10 treated wounds over controls. The beneficial effects on wound healing were probably attributed to additive enhancement of ATP production by LLLT and CoQ10 treatment. The combination of LLLT and topical CoQ10 is safe and convenient, and merits further clinical study.
authors.affiliation: ShangHai 9th People Hospital, Shanghai Jiao Tong University School of Medicine, ShangHai, China.|Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston, MA 02114.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston, MA 02114.
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Wu
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston, MA 02114.
authors.forename: Tingting
authors.initials: T
authors.lastname: Dong
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston, MA 02114.
authors.forename: Mei X
authors.initials: MX
authors.lastname: Wu
mesh_terms: D000287:Administration, Topical; D000818:Animals; D048909:Diabetes Complications / Q000473:pathology / Q000628:therapy*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D010789:Phototherapy / Q000379:methods*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D014451:Ubiquinone; C024989:coenzyme Q10
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