title: MiR-211 inhibits cell proliferation and invasion of gastric cancer by down-regulating SOX4.
abstract: BACKGROUND
  Previous studies have shown that the dysregulation of miRNAs are frequently associated with cancer progression. Deregulation of miR-211 has been observed in various types of human cancers. However, its biological function in gastric cancer (GC) is still unknown.
  
  
  METHODS
  The expression of miR-211 in GC was detected by using quantitative real-time PCR (qRT-PCR). The miR-211 mimics and inhibitor were designed and transfected into BGC-823 cells. Then, we explore the probable biological function of miR-211 in gastric cancer cell proliferation and invasion in vitro. A luciferase reporter assay and western blot were performed to confirm the target gene of miR-211.
  
  
  RESULTS
  MiR-211 was significantly down-regulated in GC. Over-expression of miR-211 inhibited gastric cancer cell proliferation and invasion in vitro, conversely, down-regulated expression of miR-211 promoted gastric cancer cell proliferation and invasion. In addition, the sex-determining region Y-related high mobility group box 4 (SOX4) is identified as a target of miR-211 in GC cells, and SOX4 expression levels was inversely correlated with miR-211. Furthermore, knockdown of Sox4 inhibited the proliferation and invasion in GC cells.
  
  
  CONCLUSIONS
  miR-211 could inhibit GC cell proliferation and invasion partially by down-regulating SOX4. MiR-211 might be a potential therapeutic target for GC treatment in the future.
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Long
authors.initials: L
authors.lastname: Hua
authors.affiliation: School of Nursing, Xinxiang Medical University Xinxiang 453000, Henan Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Kun-Hou
authors.initials: KH
authors.lastname: Yao
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Hu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014162:Transfection
chemical_list: C549405:MIRN211 microRNA, human; D035683:MicroRNAs; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors
keywords: Gastric cancer; SOX4; invasion; miR-211; proliferation
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