title: Serum alpha-klotho concentrations in girls with anorexia nervosa.
abstract: BACKGROUND
  Soluble α-klotho may influence energy homeostasis. It also plays a role in calcium-phosphate and vitamin D3 metabolism regulation. Two so far published studies have demonstrated that serum α-klotho levels in patients with AN are decreased, but their relationships with BMI and metabolic disturbances in these patients remain unclear.
  
  
  OBJECTIVE
  The aim of the study was to assess the association between serum soluble α-klotho levels and glucose, calcium-phosphorus and vitamin D3 metabolism in girls with acute AN.
  
  
  METHODS
  Serum soluble α-klotho concentrations were evaluated using commercially available ELISA kit in 31 Polish girls with restrictive AN and 29 healthy controls (C). Moreover, anthropometric measurements (weight, height, BMI) and laboratory assays (serum fasting glucose, insulin, HOMA-IR, total calcium, phosphorus as well as 25-hydroxy vitamin D3 and calcitriol) were performed.
  
  
  RESULTS
  The mean serum α-klotho concentrations in the AN group were significantly lower than in the C group even after adjustment for BMI. Significant correlations between serum α-klotho and body mass (r=0.54; p=0.009), BMI (r=0.48; p=0.02), serum calcitriol (r=0.48; p=0.03), insulin (r=0.49; p=0.008) and HOMA-IR (r=0.54; p=0.006) were observed in the AN, but not in healthy controls.
  
  
  CONCLUSIONS
  Serum α-klotho concentrations in female adolescents with AN are decreased in comparison with normal weight girls and strongly associated with their nutritional status, insulin sensitivity and active vitamin D3 levels.
authors.affiliation: Department of Pediatrics, School of Medicine with Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Oswiecimska
authors.affiliation: Department of Pediatrics, Regional Hospital in Strzelce Opolskie, Strzelce Opolskie, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Pys-Spychala
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Swietochowska
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Ostrowska
authors.affiliation: Department of Pediatric Endocrinology, University Hospital No. 1 in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szymlak
authors.affiliation: Department of Physiology, Pathology and Neonatal Intensive Care, General Hospital in Gliwice, Gliwice, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Mikolajczak
authors.affiliation: Department of Pediatric Endocrinology, University Hospital No. 1 in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
authors.forename: Zaneta
authors.initials: Z
authors.lastname: Malczyk
authors.affiliation: Department of Pediatric Neurology, Center of Pediatrics and Pediatric Oncology in Chorzów, Chorzów, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Chyra