title: Synthesis of tubular nanostructures from wheat bran albumins during proteolysis with V8 protease in the presence of calcium ions.
abstract: There are very few reports on the self-assembly of peptides derived from proteins of agro industrial byproducts origin. Although it has been claimed that purity is a determining factor in peptide self-assembly, whether proteins extracted using water along with other components also form self-assembled structures is not known. The results of this work prove that albumins from wheat bran, a byproduct obtained from the milling industry, can form tubular nanostructures during their hydrolysis with the V8 protease in the presence of Ca(2+). Electron microscopy of the hydrolysate revealed that under specific conditions, long filaments are formed, which are nanotubes of several microns in length, with inner and outer diameters of 100 and 200 nm, respectively. The infrared analysis of the hydrolysate identified (-)OOC-Ca(2+) interactions and changes in beta sheet content in response to variations in protein/V8/Ca(2+) molar ratios. A model that explains the probable mechanism of the observed self-assembly is discussed.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Chaquilla-Quilca
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico. Electronic address: rbalandran@ciad.mx.
authors.forename: R R
authors.initials: RR
authors.lastname: Balandrán-Quintana
authors.affiliation: Centro de Investigación y de Estudios Avanzados del IPN, Unidad Mérida. Departamento de Física, Carretera antigua a Progreso km 6, 97310 Mérida, Yucatán, Mexico.
authors.forename: J A
authors.initials: JA
authors.lastname: Azamar-Barrios
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de Los Alimentos, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Ramos-Clamont Montfort
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: A M
authors.initials: AM
authors.lastname: Mendoza-Wilson
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: J N
authors.initials: JN
authors.lastname: Mercado-Ruiz
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: T J
authors.initials: TJ
authors.lastname: Madera-Santana
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Animal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: Y L
authors.initials: YL
authors.lastname: López-Franco
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a La Victoria km 0.6, 83304 Hermosillo, Sonora, Mexico.
authors.forename: J G
authors.initials: JG
authors.lastname: Luna-Valdez
mesh_terms: D000418:Albumins / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D004043:Dietary Fiber / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D059748:Proteolysis; D012697:Serine Endopeptidases / Q000737:chemistry*
chemical_list: D000418:Albumins; D004043:Dietary Fiber; D010455:Peptides; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D002118:Calcium
keywords: Nanoparticles; Proteolysis; Self-assembly; Three-way nanotubes; Wheat bran albumins