title: [Interaction of MIF gene -173G/C polymorphism and GPX1 gene 594C/T polymorphism with high-fat diet in ulcerative colitis].
abstract: OBJECTIVE
  To investigate the interaction of single nucleotide polymorphisms of macrophage migration inhibitory factor (MIF) gene -173G/C and glutathione peroxidase 1(GPX1) gene 594C/T polymorphisms and high-fat diet in ulcerative colitis (UC).
  
  
  METHODS
  The genetic polymorphisms of MIF -173G/C and GPX1 594C/T were determined with a polymorphism-polymerase chain reaction (PCR)-endonuclease method in peripheral blood leukocytes derived from 1500 UC cases and 1500 healthy controls.
  
  
  RESULTS
  The frequencies of MIF -173CC and GPX1 594TT were 55.60% and 55.73% in the UC cases and 16.67% and 16.47% in the healthy controls, respectively. Statistical tests also showed a significant difference in the frequencies between the two groups (P<0.01; P<0.01, respectively). Individuals carrying MIF -173CC also had a significantly higher risk of UC compared with those with MIF -173GG (OR=6.8662, 95%CI: 4.5384-9.6158). Individuals carrying GPX1 594TT had a high risk of UC (OR=7.0854, 95%CI: 4.4702-10.5283). Combined analysis showed that the percentages of MIF -173CC/GPX1 594TT in the UC and control groups were 31.00% and 2.73%, respectively (P<0.01). Individuals carrying MIF -173CC/GPX1 594TT had a high risk of UC (OR=49.0113, 95%CI: 31.7364-61.8205). The high-fat diet rate of the case group was significantly higher than that of the control group (OR=3.3248, 95%CI: 1.9461-5.0193, P<0.01), and statistic analysis suggested an interaction between high-fat diet and MIF -173CC and GPX1 594TT which increase risk of UC (γ =6.9293; γ =6.9942).
  
  
  CONCLUSIONS
  MIF -173CC and GPX1 594TT and high-fat diet are the risk factors for UC, and the significant interactions between genetic polymorphisms of MIF -173G/C, GPX1 594C/T and high-fat diet may increase the risk for UC.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China. nn21882001@aliyun.com.
authors.forename: Chaoxian
authors.initials: C
authors.lastname: Zhang
authors.forename: Like
authors.initials: L
authors.lastname: Guo
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Qin
mesh_terms: D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism* / Q000523:psychology; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D059647:Gene-Environment Interaction; D020022:Genetic Predisposition to Disease; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000094765:Glutathione Peroxidase GPX1
chemical_list: D004041:Dietary Fats; D008263:Macrophage Migration-Inhibitory Factors; D005979:Glutathione Peroxidase; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human