title: Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility.
abstract: Coating on the sperm surface, glycocalyx, plays a key role in sperm motility, maturation and fertilization. A comprehensive profile of sperm surface glycans will greatly facilitate both basic researches and clinical studies. Because of the capability of recognizing different glycan moieties, lectins are widely used in glycobiology. However, lacking high-throughput technology, limited lectins have been reported for analyzing the glycan of human sperm. In this study, we employed a lectin microarray for profiling the surface glycans of human sperm, on which 54 out of 91 lectins showed positive binding. Based on this technique, we compared lectin binding profiling of sperm with homozygous DEFB126 mutation (del/del) with that of wild type (wt/wt). DEFB126 was reported to contribute to the sialylation on sperm surface and its homozygous mutation was related to male subfertility. Six lectins (Jacalin/AIA, GHA, ACL, MPL, VVL and ABA) were found to develop lower binding affinity to sperm with del/del. Further validation showed that these lectins, especially ABA and MPL, can be potential biomarkers for clinical diagnosis of subfertility due to the mutation of DEFB126. Our research provides insight into the detection of some unexplained male subfertility, and the lectin microarray is generally applicable for infertility/subfertility sperm biomarker discovery.
authors.affiliation: Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China.
authors.forename: Aijie
authors.initials: A
authors.lastname: Xin
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Diao
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Yancheng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Changgen
authors.initials: C
authors.lastname: Shi
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Jufen
authors.initials: J
authors.lastname: Zheng
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China.
authors.forename: Guowu
authors.initials: G
authors.lastname: Chen
authors.affiliation: Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Sun
authors.affiliation: Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China.|China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.|Shanghai Key Laboratory of Reproductive Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Shi
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shengce
authors.initials: S
authors.lastname: Tao
authors.affiliation: China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China.|Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers; D015870:Gene Expression; D005838:Genotype; D006031:Glycosylation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008297:Male; D009154:Mutation; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D013094:Spermatozoa / Q000378:metabolism*; D023083:beta-Defensins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D015415:Biomarkers; C426184:DEFB126 protein, human; D037102:Lectins; D011134:Polysaccharides; D023083:beta-Defensins
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