title: MiR-155 modulates the progression of neuropathic pain through targeting SGK3.
abstract: This study aimed to illustrate the potential effects of miR-155 in neuropathic pain and its potential mechanism. Spragure-Dawley (SD) rats were used for neuropathic pain model of bilateral chronic constriction injury (bCCI) construction. Effects of miR-155 expression on pain threshold of mechanical stimuli (MWT), paw withdrawal threshold latency (PMTL) and cold threshold were analyzed. Target for miR-155 was analyzed using bioinformatics methods. Moreover, effects of miR-155 target gene expression on pain thresholds were also assessed. Compared with the controls and sham group, miR-155 was overexpressed in neuropathic pain rats (P<0.05), but miR-155 slicing could significantly decreased the pain thresholds (P<0.05). Serum and glucocorticoid regulated protein kinase 3 (SGK3) was predicted as the target gene for miR-155, and miR-155 expression was negatively correlated to SGK3 expression. Furthermore, SGK3 overexpression could significantly decreased the pain thresholds which was the same as miR-155 (P<0.05). Moreover, miR-155 slicing and SGK3 overexpression could significantly decrease the painthreshold. The data presented in this study suggested that miR-155 slicing could excellently alleviate neuropathic pain in rats through targeting SGK3 expression. miR-155 may be a potential therapeutic target for neuropathic pain treatment.
authors.affiliation: Department of Anesthesiology, Chengdu Hospital Affiliated to Zunyi Medical College and Chengdu Second People's Hospital Chengdu 610017, China.
authors.forename: Shaoxing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Chengdu Hospital Affiliated to Zunyi Medical College and Chengdu Second People's Hospital Chengdu 610017, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Anesthesiology, Chengdu Hospital Affiliated to Zunyi Medical College and Chengdu Second People's Hospital Chengdu 610017, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, Chengdu Hospital Affiliated to Zunyi Medical College and Chengdu Second People's Hospital Chengdu 610017, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Xie
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D006930:Hyperalgesia / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology / Q000517:prevention & control; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017628:Microglia / Q000201:enzymology; D009437:Neuralgia / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology / Q000517:prevention & control; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D010147:Pain Measurement; D017288:Pain Threshold; D010812:Physical Stimulation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D015398:Signal Transduction; D013116:Spinal Cord / Q000201:enzymology* / Q000503:physiopathology; D013997:Time Factors; D014162:Transfection; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D017346:Protein Serine-Threonine Kinases; C580597:MIRN155 microRNA, rat; C000611836:SGK3 protein, rat; D000099184:Serum-Glucocorticoid Regulated Kinases
keywords: Neuropathic pain; SGK3; mechanical allodynia; miR-155; thermal hyperalgesia
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