title: Evidence of intrauterine transmission of lumpy skin disease virus.
abstract: The current study describes the clinical, histopathological, molecular and serological diagnosis of lumpy skin disease (LSD) in a premature 1-day old calf that has been delivered from a cow that exhibited signs of LSD during the seventh month of pregnancy. The calf showed generalized skin lesions accompanied with signs of immaturity and died 36 h after birth. Postmortem and histopathological examinations revealed the involvement of multiple tissues. The presence of Neethling virus DNA in tissues was confirmed by polymerase chain reaction (PCR) and gene sequencing. Results of ELISA and serum neutralization test (SNT) confirmed that the calf had developed precolostral serum antibodies to LSD virus indicating in utero virus transmission. All tested sera collected from animals located in the same area were serologically positive, indicating exposure to LSD virus.
authors.affiliation: Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: sh_alrouby@yahoo.com.
authors.forename: Sherin
authors.initials: S
authors.lastname: Rouby
authors.affiliation: Department of Pox, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt.
authors.forename: Emad
authors.initials: E
authors.lastname: Aboulsoud
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D017809:Fatal Outcome; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D008166:Lumpy Skin Disease / Q000175:diagnosis* / Q000635:transmission* / Q000821:virology; D008167:Lumpy skin disease virus / Q000302:isolation & purification*; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
keywords: ELISA; Histopathology; Lumpy skin disease; Neethling virus; PCR