title: [Effect of IFN-α on hepatic cancer SMCC-7721 cell via PI3K/Akt signaling pathway and related mechanism research].
abstract: OBJECTIVE
  This study explored how interferon alpha (IFN-α) influenced liver cancer SMCC-7721 cell via PI3K/Akt signaling pathway.
  
  
  METHODS
  The effect of IFN-α on the viability of SMCC-7721 cell was measured by MTT assay. The effect of IFN-α on SMCC-7721 cell cycle was measured by flow cytometry. The activation of PI3K/Akt signaling pathway and downstream target genes expression level of glycogen synthase kinase 3 beta (GSK-3β), and cell cycle protein (Cyclin D1), p21, C-myc, Survivin were assayed by Western blot.
  
  
  RESULTS
  MTT assay indicated IFN-α (5, 10, 15 U/L) could inhibit SMCC-7721 cell viability, and the inhibitory rate was the highest at 48 h. IFN-α made SMCC-7721 cell arrested. Western blotting assays presented IFN-α could down-regulate expression of PI3K and suppress the phosphorylation of Akt, and then inhibit phosphorylation of GSK-3β. It could also reduce the expression levels of Cyclin D1, C-myc and Survivin but increase the level of p21.
  
  
  CONCLUSIONS
  These results suggested IFN-α could depress the growth and proliferation of liver cancer SMCC-7721 cell via blocking PI3K/Akt signaling pathway, and the molecular proteins Cyclin D1, p21, C-myc, Survivin were involved.
authors.affiliation: Cancer Therapy Center, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Email: octflower79@sina.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.affiliation: Cancer Therapy Center, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Guangyong
authors.initials: G
authors.lastname: Shi
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D038362:Glycogen Synthase Kinase 3; D000071679:Glycogen Synthase Kinase 3 beta; D022781:Hepatocytes; D006801:Humans; D016898:Interferon-alpha; D008113:Liver Neoplasms; D019869:Phosphatidylinositol 3-Kinases; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt; D015398:Signal Transduction
chemical_list: D018797:Cell Cycle Proteins; D016898:Interferon-alpha; D019869:Phosphatidylinositol 3-Kinases; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3