title: miR-146a and miR-638 in BRCA1-deficient triple negative breast cancer tumors, as potential biomarkers for improved overall survival.
abstract: BACKGROUND
  Mechanisms that lead to the reduced expression of BRCA1 in triple-negative breast cancer (TNBC) tumors are not fully understood. A possible cause is overexpression of miR-146a and miR-638, which regulate BRCA1 expression in other cancers.
  
  
  OBJECTIVE
  To evaluate the expression of these microRNAs in relation to BRCA1 expression in TNBC tumors.
  
  
  METHODS
  Expression of both microRNAs was assessed by real time qPCR using Taqman microRNA assays in TNBC tumors. Results were related to protein expression of BRCA1 and patient's survival.
  
  
  RESULTS
  miR-146a and miR-638 were overexpressed in 36% and 59% of TNBC tumors, respectively. Overexpression was preeminent in BRCA1-deficient tumors and significantly associated to a better overall survival.
  
  
  CONCLUSIONS
  Both miRNAs are potential biomarkers for improved overall survival in patients with BRCA1-deficient TNBC tumors.
authors.affiliation: Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Valentina
authors.initials: V
authors.lastname: Zavala
authors.affiliation: Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Elisa
authors.initials: E
authors.lastname: Pérez-Moreno
authors.affiliation: Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Teresa
authors.initials: T
authors.lastname: Tapia
authors.affiliation: Centro de Cáncer, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Camus
authors.affiliation: Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Pilar
authors.initials: P
authors.lastname: Carvallo
mesh_terms: D000328:Adult; D000368:Aged; D019313:BRCA1 Protein / Q000172:deficiency*; D024682:BRCA2 Protein / Q000235:genetics; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018095:Germ-Line Mutation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D064726:Triple Negative Breast Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality*; D055815:Young Adult
chemical_list: D019313:BRCA1 Protein; D024682:BRCA2 Protein; D014408:Biomarkers, Tumor; C542542:MIRN146 microRNA, human; C572154:MIRN638 microRNA, human; D035683:MicroRNAs; D066246:ErbB Receptors
keywords: BRCA1; breast cancer; miR-146a; miR-638; microRNA; triple negative breast cancer