title: Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).
abstract: Real-time quantitative reverse transcription PCR technique has advanced greatly over the past 20 years. Messenger RNA (mRNA) levels in cells or tissues can be quantified by this approach. It is well known that changes in mRNA expression in disease, and correlation of mRNA expression profiles with clinical parameters, serve as clinically relevant biomarkers. Hence, accurate determination of the mRNA levels is critically important in describing the biological, pathological, and clinical roles of genes in health and disease. This chapter describes a real-time PCR approach to detect and quantify mRNA expression levels, which can be used for both laboratorial and clinical studies in breast cancer research.
authors.affiliation: Division of Cancer Prevention, Department of Medicine, Stony Brook University, Stony Brook, NY, 11794, USA.|Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Cancer Prevention, Department of Medicine, Stony Brook University, Stony Brook, NY, 11794, USA.|Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. zhoujie01@medmail.com.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
mesh_terms: D018076:DNA, Complementary / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D048348:Reverse Transcription
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger
keywords: RNA isolation; Real-time PCR; Reverse transcription; mRNA