title: Lung tissue apoptosis in abdominal hypertension : Apoptosis and necrosis of lung tissue in abdominal hypertension.
abstract: OBJECTIVE
  The aim of this study was to evaluate lung tissue histopathologic changes and the number of apoptosis with the increase of abdominal pressure.
  
  
  METHODS
  The study rats were randomly assigned into the following five groups: a sham operated group and groups 1, 2, 3 and 4, in which the intra-abdominal pressure was increased to 11, 15, 18 and 22 mmHg for 60 min, respectively. Lungs were harvested for histopathologic changes and the tissue apoptotic analysis were carried out in a blinded manner.
  
  
  RESULTS
  All of the data showed that the number of apoptotic cells and necrosis were increased in accordance with the pressure level. However, this increase was statistically significant, especially in groups 3 and 4 (18 and 22 mmHg, respectively; p < 0.05) when compared to the sham operated rats. There were no differences observed between groups 1 and 2 (11 and 15 mmHg, respectively) and the sham operated rats. There was also no difference between groups 1 and 2. There were findings of coagulation necrosis and the number of apoptotic cells linearly increased when the abdominal pressure was increased. The cut-off value was 15 mmHg.
  
  
  CONCLUSIONS
  The available findings suggest that intra-abdominal pressure greater than 15 mmHg could irreversibly damage pulmonary cells and both coagulation necrosis parameters and the number of apoptosis increase in accordance with the pressure level.
authors.affiliation: Sakarya Üniversitesi, Sakarya Eğitim Araştırma Hastanesi, Korucuk Kampüsü, 3. kat, Sakarya, 54200, Turkey. akbulutg@asia.com.
authors.forename: G
authors.initials: G
authors.lastname: Akbulut
authors.affiliation: Department of General Surgery, Kocatepe Üniversitesi, Mavi Hastane, Ozdilek yolu 9. km, Afyonkarahisar, 03200, Turkey.
authors.forename: M B
authors.initials: MB
authors.lastname: Yazicioglu
authors.affiliation: Department of Pathology, Namık Kemal Üniversitesi, Tekirdağ, Turkey.
authors.forename: Ö
authors.initials: Ö
authors.lastname: Şahin
authors.affiliation: Department of Histology and Embriology, Kocatepe Üniversitesi, Mavi Hastane, Ozdilek yolu 9. km, Afyonkarahisar, 03200, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Tosun
authors.affiliation: Sakarya Üniversitesi, Sakarya Üniversitesi Tıp Fakültesi, Esentepe, Sakarya, 54100, Turkey. ondilek@hotmail.com.
authors.forename: O N
authors.initials: ON
authors.lastname: Dilek
keywords: Abdominal; Experimental trauma surgery; General trauma; Open abdomen; Thoracic; Thoracic and abdominal
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