title: Association of Decreased Expression of Serum miR-9 with Poor Prognosis of Oral Squamous Cell Carcinoma Patients.
abstract: BACKGROUND
  Dysregulation of miR-9 is a common feature of many types of cancers, including oral squamous cell carcinoma (OSCC). However, whether the expression level of serum miR-9 is changed in patients with OSCC remains unknown.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine the expression level of serum miR-9 in OSCC patients, oral leukoplakia (OLK) patients, and healthy volunteers, then we evaluated the association between serum miR-9 expression level and clinical outcome of OSCC patients.
  
  
  RESULTS
  The expression level of serum miR-9 was significantly downregulated in patients with OSCC or OLK in comparison with healthy controls (P<0.01). Serum miR-9 expression level was associated with various clinicopathological parameters, including T stage (P=0.013), lymph node metastasis (P=0.002), and TNM stage (P=0.007). In addition, the OSCC patients in the low serum miR-9 expression group had poorer overall survival rate (P=0.022) and disease-free survival rate (P=0.004) compared with those in the high serum miR-9 expression group. Multivariate analysis showed that serum miR-9 was an independent prognostic factor for OSCC.
  
  
  CONCLUSIONS
  Serum miR-9 was downregulated in patients with OSCC and patients with OLK. In addition, low serum miR-9 was correlated with poor prognosis of OSCC, indicating miR-9 might play a tumor suppressive role in OSCC and can serve as a promising biomarker for this deadly disease.
authors.affiliation: Department of Oral Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, China (mainland).
authors.forename: Legang
authors.initials: L
authors.lastname: Sun
authors.affiliation: Room of Gastroscope, The Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, China (mainland).
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Oral Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, China (mainland).
authors.forename: Honghai
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Rehabilitation, The Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, China (mainland).
authors.forename: Qiuqin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Oral Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, China (mainland).
authors.forename: Yao
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007971:Leukoplakia / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D015999:Multivariate Analysis; D011379:Prognosis; D012307:Risk Factors
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs
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