title: Effects of deoxynivalenol on calcium homeostasis of concanavalin A--Stimulated splenic lymphocytes of chickens in vitro.
abstract: In this study, the in vitro effects of the treatment of concanavalin A (Con A)--stimulated splenic lymphocytes with DON were examined. Splenic lymphocytes isolated from chickens were stimulated with 12.5 μg/mL Con A and exposed to deoxynivalenol (DON) (0-50 μg/mL) for 48 h. The intracellular calcium concentration ([Ca(2+)]i), pH, calmodulin (CaM) mRNA levels, and Na(+),K(+)-ATPase and Ca(2+)-ATPase activities were detected. With the DON exposure concentrations increased, the [Ca(2+)]i and CaM mRNA levels gradually increased in a dose-dependent manner, and all the evaluated conconcentrations affected ATPase activity to the same extent. There were significant differences (P<0.05 or P<0.01) between the treatment groups and the control group. These results indicate that an imbalance in calcium homeostasis and intracellular acidification are components of DON cytotoxicity in chicken lymphocytes.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: zhihua_ren@126.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China. Electronic address: wangyachao1688@126.com.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: 847767028@qq.com.
authors.forename: Huidan
authors.initials: H
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: 335824070@qq.com.
authors.forename: Youtian
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: dengjl213@126.com.
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: zzcjl@126.com.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: wangyadudu@126.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: pengxi197313@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: cui580420@sicau.edu.cn.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: shenliuhong@sohu.com.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: yayushumin@163.com.
authors.forename: Shumin
authors.initials: S
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: caosuizhong@yahoo.com.cn.
authors.forename: Suizhong
authors.initials: S
authors.lastname: Cao
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D006706:Homeostasis / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D002147:Calmodulin; D004785:Environmental Pollutants; D014255:Trichothecenes; D003208:Concanavalin A; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; C007262:deoxynivalenol; D002118:Calcium
keywords: ATPase; CaM; Calcium homeostasis; Deoxynivalenol; Mycotoxin