title: Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma.
abstract: BACKGROUND
  Promoter hypermethylation is a frequent epigenetic mechanism for gene transcription repression in cancer and is one of the hallmarks of the disease. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) contributes to cell contact-mediated communication. Dysregulation of promoter methylation has been reported in various cancers.
  
  
  OBJECTIVE
  The objectives of this study were to investigate the CELSR3 hypermethylation level in oral squamous cell carcinomas (OSCCs) using methylation-sensitive high-resolution melting analysis (MS-HRM) and to correlate CELSR3 methylation with patient demographic and clinicopathological parameters.
  
  
  METHODS
  Frozen tissue samples of healthy subjects' normal mucosa and OSCCs were examined with regard to their methylation levels of the CELSR3 gene using MS-HRM.
  
  
  RESULTS
  MS-HRM analysis revealed a high methylation level of CELSR3 in 86% of OSCC cases. Significant correlations were found between CELSR3 quantitative methylation levels with patient ethnicity (P=0.005), age (P=0.024) and pathological stages (P=0.004). A moderate positive correlation between CELSR3 and patient age was also evident (R=0.444, P=0.001).
  
  
  CONCLUSIONS
  CELSR3 promoter hypermethylation may be an important mechanism involved in oral carcinogenesis. It may thus be used as a biomarker in OSCC prognostication.
authors.affiliation: Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buluh Campus, Malaysia E-mail : gootheah@salam.uitm.edu.my.
authors.forename: Goot Heah
authors.initials: GH
authors.lastname: Khor
authors.forename: Gabrielle Ruth Anisah
authors.initials: GR
authors.lastname: Froemming
authors.forename: Rosnah Binti
authors.initials: RB
authors.lastname: Zain
authors.forename: Thomas Mannil
authors.initials: TM
authors.lastname: Abraham
authors.forename: Thong Kwai
authors.initials: TK
authors.lastname: Lin
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D015820:Cadherins / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C559812:Celsr3 protein, human; D004273:DNA, Neoplasm; D011956:Receptors, Cell Surface