title: Regulation of CCAAT/enhancer-binding protein (C/EBP) α in human-cytomegalovirus-infected fibroblasts.
abstract: CCAAT/enhancer-binding protein (C/EBP) α, a member of the C/EBP family of transcription factors, is known to be involved in gene expression and DNA replication of human cytomegalovirus (HCMV). This study aimed to understand the regulation of endogenous C/EBPα during HCMV infection using an in vitro infection model. The expression and localization of C/EBPα were investigated in fibroblasts infected with HCMV. The overexpression of C/EBP homologous protein (CHOP), the endogenous inhibitor of C/EBP, was also employed to test the involvement of C/EBPα during HCMV infection. Our data showed that HCMV infection increases the expression of the full-length C/EBPα isoform (p42) especially during the late stage of infection at the transcriptional and post-translational levels. The increased p42 accumulated in the viral DNA replication compartment. p42 expression was not induced in cells treated with UV-irradiated virus or in cells infected with normal virus in the presence of ganciclovir. CHOP-mediated inhibition of C/EBP activity suppressed viral gene expression and DNA replication, which lowered the level of viral production. Together, our data suggest that HCMV-mediated C/EBPα regulation might play a beneficial role in the lytic cycle of HCMV.
authors.affiliation: Laboratory of Virology, School of Biological Sciences, Seoul National University, Seoul, 151-747, Korea.
authors.forename: Junsub
authors.initials: J
authors.lastname: Lee
authors.affiliation: Laboratory of Virology, School of Biological Sciences, Seoul National University, Seoul, 151-747, Korea. sunyoung@snu.ac.kr.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Kim
mesh_terms: D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000502:physiology*; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000821:virology; D005347:Fibroblasts / Q000821:virology; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000502:physiology; D057809:HEK293 Cells / Q000821:virology; D006801:Humans; D015151:Immunoblotting; D060888:Real-Time Polymerase Chain Reaction; D014779:Virus Replication / Q000502:physiology
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha