title: Effect of Jagged-1 and Dll-1 on osteogenic differentiation by stem cells from human exfoliated deciduous teeth.
abstract: OBJECTIVE
  The aim of the present study was to determine the influence of Notch ligands, Jagged-1 and Dll-1, on osteogenic differentiation by stem cells from human exfoliated deciduous teeth.
  
  
  METHODS
  Notch ligands were immobilized on tissue culture surface using an indirect affinity immobilization technique. Cells from the remaining of dental pulp tissues from human deciduous teeth were isolated and characterized using flow cytometry and differentiation assay. Alkaline phosphatase (ALP) enzymatic activity, osteogenic marker gene expression, and mineralization were determined using ALP assay, real-time polymerase chain reaction, and alizarin red staining, respectively.
  
  
  RESULTS
  The isolated cells exhibited CD44, CD90, and CD105 expression but lack of CD45 expression. Further, these cells were able to differentiate toward osteogenic lineage. The upregulation of HES-1 and HEY-1 was observed in those cells on Dll-1 and Jagged-1 coated surface. The significant increase of ALP activity and mineralization was noted in those cells seeded on Jagged-1 surface and these results were attenuated when cells were pretreated with gamma secretase inhibitor. The significant upregulation of ALP and collagen type I gene expression was also observed in those cells seeded on Jagged-1 surface. The inconsistent Dll-1 induced osteogenic differentiation was found and high Dll-1 immobilized dose (50 nM) slightly enhanced alkaline phosphatase enzymatic activity. However, the statistical significant difference was not obtained as compared to the hFc control.
  
  
  CONCLUSIONS
  The surface immobilization of Notch ligands, Jagged-1 and Dll-1, likely to enhance osteogenic differentiation of SHEDs. However, Jagged-1 had more ability in enhancing osteogenic differentiation than Dll-1 in our model.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand; Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand. Electronic address: wsukarawan@hotmail.com.
authors.forename: Waleerat
authors.initials: W
authors.lastname: Sukarawan
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand; Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand.
authors.forename: Karnnapas
authors.initials: K
authors.lastname: Peetiakarawach
authors.affiliation: Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand; Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand.
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
authors.affiliation: Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand; Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand. Electronic address: thanaphum.o@chula.ac.th.
authors.forename: Thanaphum
authors.initials: T
authors.lastname: Osathanon
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002113:Calcification, Physiologic; D018797:Cell Cycle Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000494:pharmacology*; D000072100:Jagged-1 Protein / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods; D014094:Tooth, Deciduous / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D000072056:Transcription Factor HES-1 / Q000235:genetics; D015854:Up-Regulation
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D024042:Collagen Type I; D055767:Immobilized Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D000072056:Transcription Factor HES-1; C485631:HEY1 protein, human; C000606273:JAG1 protein, human; C078493:HES1 protein, human
keywords: Differentiation; Notch ligand; Osteoblast; Stem cells from human exfoliated deciduous teeth