title: Conductive PEDOT:PSS coated polylactide (PLA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) electrospun membranes: Fabrication and characterization.
abstract: In the current study, electrospinning technique was used to fabricate composite membranes by blending of a synthetic polymer, polylactic acid (PLA) and a natural polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), PHBV. Conductive membranes were prepared by dipping PLA/PHBV electrospun membranes into poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (
  PSS) solution, which is a biocompatible polymer. The coated and uncoated membranes were evaluated using several techniques. The electrical conductivity of the coated membranes was measured using a digital multimeter. In vitro cell cytotoxicity and cell viability were measured by culturing human skin fibroblast (HSF) cells onto the membranes using MTT assays. It was observed that electrospinning of 20% (w/v) PLA/PHBV with a weight ratio of 50:50 produced the most uniform fibers with no beads. It was observed that the wettability and surface roughness of the
  PSS coated PLA/PHBV membranes were greatly increased than uncoated membrane. The results of cell viability using MTT assay, cell attachment and cell proliferation showed that the conductive
  PSS coated PLA/PHBV membrane were more favorable for tissue engineering application than their uncoated counterparts.
authors.affiliation: Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81300, Johor, Malaysia.
authors.forename: Hui Chung
authors.initials: HC
authors.lastname: Chang
authors.affiliation: Miniaturized Medical Devices Program, Institute of Microelectronics, Agency for Science, Technology and Research (A* STAR), Singapore.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81300, Johor, Malaysia; Advanced Membrane Technology Research Center, Universiti Teknologi Malaysia, 81300, Johor, Malaysia. Electronic address: naznin@biomedical.utm.my.
authors.forename: Naznin
authors.initials: N
authors.lastname: Sultana
authors.affiliation: Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81300, Johor, Malaysia.
authors.forename: Mim Mim
authors.initials: MM
authors.lastname: Lim
authors.affiliation: KALAM, Faculty of Alam Bina, Universiti Teknologi Malaysia, 81300, Johor, Malaysia.
authors.forename: Tareef Hayat
authors.initials: TH
authors.lastname: Khan
authors.affiliation: Advanced Membrane Technology Research Center, Universiti Teknologi Malaysia, 81300, Johor, Malaysia.
authors.forename: Ahmad Fauzi
authors.initials: AF
authors.lastname: Ismail
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004553:Electric Conductivity; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013876:Thiophenes / Q000737:chemistry*; D017155:Wettability; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011137:Polystyrenes; D013876:Thiophenes; C533756:poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate); C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C033616:poly(lactide)
keywords: Electrospinning; PEDOT:PSS; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Polylactide; Scaffold