title: Being Two Is Better than Being One: A Facile Strategy to Fabricate Multicomponent Nanoparticles for Efficient Gene Delivery.
abstract: Multifunctionality is necessary in the design of efficient gene vectors due to the existence of multiple barriers during gene delivery. Traditional methods in the design of polyfunctional materials for this purpose are associated with sophisticated syntheses and high costs. Here, we proposed a facile coassembly approach to fabricate multicomponent nanoparticles for efficient gene delivery. The resulting particles contain different functional dendrimers and show favorable physicochemical characteristics. All the combinations in the fabrication of multicomponent nanoparticles show a synergistic effect in improving transfection efficacy. The prepared nanoparticles successfully address two or more barriers in the gene delivery process and show minimal toxicity on the transfected cells. The combination of high transfection efficacy and low cytotoxicity suggests that the prepared multicomponent nanoparticles as promising carriers in gene delivery.
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University , Shanghai 200241, China.|Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiaotong University , Shanghai 200025, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiaotong University , Shanghai 200025, China.
authors.forename: Lianfu
authors.initials: L
authors.lastname: Deng
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University , Shanghai 200241, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University , Shanghai 200241, China.
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles