title: Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles.
abstract: Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biointerfaces, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland. greta.faccio@empa.ch michael.richter@igb.fraunhofer.de.
authors.forename: G
authors.initials: G
authors.lastname: Faccio
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Protection and Physiology, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland.
authors.forename: M B
authors.initials: MB
authors.lastname: Bannwarth
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biointerfaces, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland. greta.faccio@empa.ch michael.richter@igb.fraunhofer.de.
authors.forename: C
authors.initials: C
authors.lastname: Schulenburg
authors.affiliation: IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425 Jülich, DE, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Steffen
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biointerfaces, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland. greta.faccio@empa.ch michael.richter@igb.fraunhofer.de.
authors.forename: D
authors.initials: D
authors.lastname: Jankowska
authors.affiliation: IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425 Jülich, DE, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Pohl
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Protection and Physiology, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland.
authors.forename: R M
authors.initials: RM
authors.lastname: Rossi
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biointerfaces, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland. greta.faccio@empa.ch michael.richter@igb.fraunhofer.de.
authors.forename: K
authors.initials: K
authors.lastname: Maniura-Weber
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Protection and Physiology, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland.
authors.forename: L F
authors.initials: LF
authors.lastname: Boesel
authors.affiliation: Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biointerfaces, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland. greta.faccio@empa.ch michael.richter@igb.fraunhofer.de.
authors.forename: M
authors.initials: M
authors.lastname: Richter
mesh_terms: D015374:Biosensing Techniques; D031541:Fluorescence Resonance Energy Transfer; D005947:Glucose / Q000032:analysis*; D008320:Maltose / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide
chemical_list: D008320:Maltose; D012822:Silicon Dioxide; D005947:Glucose