title: Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection.
abstract: Flavonoids are important active ingredients in many traditional Chinese medicines. In this paper, capillary electrophoresis with amperometric detection was employed to separate and detect eight flavonoids, rutin, quercetrin, quercetin, kaempferol, kaempferide, catechin, apigenin, and luteolin, in a home-made capillary electrophoresis device. Under the separation voltage of 2000 V, the eight flavonoids could be completely separated within 33 min in 18 mM borax running buffer at pH 10.2. Good linear relationships were obtained for all analytes and the detection limits for flavonoids ranged from 0.46 to 0.85 μM. Then, the method was applied to separate and determine the flavonoids in three traditional Chinese medicines, hippophae rhamnoides, hypericum perforatum, and cacumen platycladi. Finally, rutin, kaempferol, quercetin, and quercetrin were discovered in these medicines and the concentrations ranged from 0.28 to 9.94 mg/g. The recoveries of flavonoids ranged from 84.7 to 113%, which showed the high reliability of this method.
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, School of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, School of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, School of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Ma
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, School of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
authors.forename: Kaixuan
authors.initials: K
authors.lastname: Xu
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, School of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Lin
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D019075:Electrophoresis, Capillary; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids
keywords: Amperometric detection; Capillary electrophoresis; Flavonoids; Traditional Chinese medicine