title: Both Talin-1 and Talin-2 correlate with malignancy potential of the human hepatocellular carcinoma MHCC-97 L cell.
abstract: BACKGROUND
  Talin-1 (TLN-1) and TLN-2 are implicated in many cellular processes, but their roles in hepatocellular carcinoma (HCC) remain unclear. This study aimed to assess cell cycle distribution, anoikis, invasion and migration in human HCC MHCC-97 L cells.
  
  
  METHODS
  MHCC-97 L cells, which highly express TLN-1, were transduced with TLN-1 shRNA (experimental group) or scramble shRNA (negative control group); non-transduced MHCC-97 L cells were used as blank controls. TLN-1 and TLN-2 mRNA and protein levels were detected by real-time RT-PCR and western blot, respectively. Then, cell cycle distribution and anoikis were assessed by flow cytometry. In addition, migration and invasion abilities were assessed using Transwell and cell scratch assays. Finally, a xenograft nude mouse model was established to further assess cell tumorigenicity.
  
  
  RESULTS
  Compared with the blank and negative control groups, TLN-1/2 mRNA and protein levels were significantly reduced in the experiment group. TLN-1/2 knockdown cells showed significantly more cells in the G0/G1 phase (79.24%) in comparison with both blank (65.36%) and negative (62.69%) control groups; conversely, less cells were found in G2/M and S phases in the experimental group compared with controls. Moreover, anoikis was enhanced (P < 0.05), while invasion and migration abilities were reduced (P < 0.05) in TLN-1/2 knockdown cells compared with controls. TLN-1/2 knockdown inhibited MHCC-97 L cell migration (Percentage of wound healing area: experimental group: 32.6 ± 0.7% vs. negative controls: 50.1 ± 0.6% and blank controls: 53.6 ± 0.6%, both P < 0.01). Finally, the tumors obtained with TLN-1/2 knockdown cells were smaller (P < 0.05) compared with controls.
  
  
  CONCLUSIONS
  Both TLN-1 and TLN-2 levels correlate with tumorigenicity in human HCC, indicating that these molecules constitute important molecular targets for the diagnosis and/or treatment of HCC.
authors.affiliation: The People's Hospital, Xuancheng City, Auhui Province, China.
authors.forename: Kun-Peng
authors.initials: KP
authors.lastname: Fang
authors.affiliation: First Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
authors.affiliation: First Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui Province, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Ren
authors.affiliation: First Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui Province, China.
authors.forename: Ye-Chuan
authors.initials: YC
authors.lastname: Xu
authors.affiliation: First Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui Province, China.
authors.forename: She-Min
authors.initials: SM
authors.lastname: Zhang
authors.affiliation: The People's Hospital, Xuancheng City, Auhui Province, China. qianyeben@hotmail.com.|First Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui Province, China. qianyeben@hotmail.com.
authors.forename: Ye-Ben
authors.initials: YB
authors.lastname: Qian
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D016608:Talin / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C518832:TLN1 protein, human; C436513:TLN2 protein, human; D016608:Talin
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