title: Characterization and application of roxithromycin loaded cyclodextrin based nanoparticles for treatment of multidrug resistant bacteria.
abstract: An outbreak of infections with a high mortality rate caused by multidrug resistant (MDR) bacteria is one of the biggest health challenges globally. A class IV drug, roxithromycin (ROX), has poor solubility. In this study, ROX was first encapsulated in the cavity of each of the β-cyclodextrin (βCD) and hydroxypropyl-β-cyclodextrin (HPβCD). Then, each of the resulting βCD-ROX inclusion complex and HPβCD-ROX inclusion complex were separately loaded into poly-(lactic-co-glycolic acid) (PLGA) to synthesize βCD-ROX/PLGA and HPβCD-ROX/PLGA nanoparticles (NPs). Blank and ROX loaded PLGA (ROX-PLGA) NPs were also prepared. The loading efficiency of ROX is comparatively high for HPβCD-ROX/PLGA NPs in comparison to the βCD-ROX/PLGA NPs and ROX-PLGA NPs. All designed formulations showed significant (P<0.0001) antibacterial activity against the selected MDR bacterial strains. In a nutshell, this study demonstrated a great therapeutic potential of the above-mentioned delivery systems for treatment of MDR bacteria.
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan. Electronic address: farha.masood@yahoo.com.
authors.forename: Farha
authors.initials: F
authors.lastname: Masood
authors.affiliation: Department of Metallurgy and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.
authors.forename: Tariq
authors.initials: T
authors.lastname: Yasin
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan.
authors.forename: Habib
authors.initials: H
authors.lastname: Bukhari
authors.affiliation: School of Chemicals & Materials Engineering (SCME), National University of Sciences and Technology (NUST), Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mujahid
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D053758:Nanoparticles / Q000737:chemistry*; D015575:Roxithromycin / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003505:Cyclodextrins; D015575:Roxithromycin
keywords: Antimicrobial activity; Cyclodextrin; Inclusion complex; Nanoparticles; Poly-(lactic-co-glycolic acid); Roxithromycin