title: Bioactive treatment promotes osteoblast differentiation on titanium materials fabricated by selective laser melting technology.
abstract: Selective laser melting (SLM) technology is useful for the fabrication of porous titanium implants with complex shapes and structures. The materials fabricated by SLM characteristically have a very rough surface (average surface roughness, Ra=24.58 µm). In this study, we evaluated morphologically and biochemically the specific effects of this very rough surface and the additional effects of a bioactive treatment on osteoblast proliferation and differentiation. Flat-rolled titanium materials (Ra=1.02 µm) were used as the controls. On the treated materials fabricated by SLM, we observed enhanced osteoblast differentiation compared with the flat-rolled materials and the untreated materials fabricated by SLM. No significant differences were observed between the flat-rolled materials and the untreated materials fabricated by SLM in their effects on osteoblast differentiation. We concluded that the very rough surface fabricated by SLM had to undergo a bioactive treatment to obtain a positive effect on osteoblast differentiation.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University.
authors.forename: Masako
authors.initials: M
authors.lastname: Tsukanaka
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Fujibayashi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Takemoto
authors.forename: Tomiharu
authors.initials: T
authors.lastname: Matsushita
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kokubo
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kiyoyuki
authors.initials: K
authors.lastname: Sasaki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D007834:Lasers; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D013778:Tetrazolium Salts; D014025:Titanium / Q000737:chemistry; D017155:Wettability
chemical_list: D003470:Culture Media; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D014025:Titanium