title: Symbiotic Bacteria in Gills and Guts of Chinese Mitten Crab (Eriocheir sinensis) Differ from the Free-Living Bacteria in Water.
abstract: Aquatic animals have a close relationship with water, but differences in their symbiotic bacteria and the bacterial composition in water remains unclear. Wild or domestic Chinese mitten crabs (Eriocheir sinensis) and the water in which they live were collected from four sampling sites in Jiangsu and Shanghai, China. Bacterial composition in water, gills or guts of E. sinensis, were compared by high-throughput sequencing using 16S rRNA genes. Analysis of >660,000 sequences indicated that bacterial diversity was higher in water than in gills or guts. Tenericutes and Proteobacteria were dominant phyla in guts, while Actinobacteria, Proteobacteria and Bacteroidetes were dominant in gills and water. Non-metric multidimensional scaling analysis indicated that microbiota from gills, guts or water clearly separated into three groups, suggesting that crabs harbor a more specific microbial community than the water in which they live. The dominant OTUs in crab gut were related to Mycoplasmataceae, which were low in abundance in gills, showing that, like mammals, crabs have body-site specific microbiota. OTUs related to Ilumatobacter and Albimonas, which are commonly present in sediment and seawater, were dominant in gills but almost absent from the sampled water. Considering E. sinensis are bottom-dwelling crustacean and they mate in saline water or seawater, behavior and life cycle of crabs may play an important role in shaping the symbiotic bacterial pattern. This study revealed the relationship between the symbiotic bacteria of Chinese mitten crab and their habitat, affording information on the assembly factors of commensal bacteria in aquatic animals.
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Liqiao
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Cai
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Qiao
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Du
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Erchao
authors.initials: E
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003386:Brachyura / Q000382:microbiology* / Q000502:physiology; D002681:China; D000069196:Gastrointestinal Microbiome; D005880:Gills / Q000382:microbiology* / Q000502:physiology; D064307:Microbiota; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013559:Symbiosis; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
references.citation: Cell. 2006 Feb 24;124(4):837-48
references.pmid: 16497592
references.citation: Microb Ecol. 2016 Jan;71(1):194-206
references.pmid: 26311127
references.citation: Appl Environ Microbiol. 2010 Feb;76(3):631-9
references.pmid: 19948856
references.citation: Nat Methods. 2010 May;7(5):335-6
references.pmid: 20383131
references.citation: Bioinformatics. 2011 Aug 15;27(16):2194-200
references.pmid: 21700674
references.citation: Science. 2011 Nov 4;334(6056):670-4
references.pmid: 22053049
references.citation: FEMS Microbiol Ecol. 2012 Mar;79(3):568-80
references.pmid: 22092438
references.citation: PLoS One. 2012;7(2):e30440
references.pmid: 22363439
references.citation: Mol Ecol. 2012 Jul;21(13):3363-78
references.pmid: 22486918
references.citation: Microb Ecol. 2012 May;63(4):938-53
references.pmid: 21915632
references.citation: Cell Host Microbe. 2012 Sep 13;12(3):277-88
references.pmid: 22980325
references.citation: PLoS One. 2013;8(4):e60802
references.pmid: 23577162
references.citation: Genes Dev. 2013 Apr 1;27(7):701-18
references.pmid: 23592793
references.citation: FEMS Microbiol Ecol. 2013 Sep;85(3):483-94
references.pmid: 23607777
references.citation: Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3404-8
references.pmid: 23524358
references.citation: Appl Environ Microbiol. 2013 Oct;79(19):5962-9
references.pmid: 23872556
references.citation: Nat Methods. 2013 Oct;10(10):996-8
references.pmid: 23955772
references.citation: Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3597-601
references.pmid: 23606476
references.citation: ISME J. 2014 Mar;8(3):541-51
references.pmid: 24132079
references.citation: Mol Ecol. 2014 Mar;23(6):1457-72
references.pmid: 23952239
references.citation: Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):E5096-104
references.pmid: 25385629
references.citation: Mol Ecol. 2015 Feb;24(3):656-72
references.pmid: 25533191
references.citation: Fish Shellfish Immunol. 2015 Mar;43(1):264-74
references.pmid: 25559445
references.citation: PLoS One. 2015;10(4):e0123990
references.pmid: 25875449
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2015 Aug;186:59-67
references.pmid: 25934083
references.citation: Environ Sci Pollut Res Int. 2015 Aug;22(16):12605-14
references.pmid: 25911286
references.citation: Environ Microbiol. 2015 Oct;17(10):3914-24
references.pmid: 25923170
references.citation: Nat Med. 2009 Dec;15(12):1377-82
references.pmid: 19966777