title: Pleurotus eryngii Polysaccharide Promotes Pluripotent Reprogramming via Facilitating Epigenetic Modification.
abstract: Pleurotus eryngii is a medicinal/edible mushroom with great nutritional value and bioactivity. Its polysaccharide has recently been developed into an effective gene vector via cationic modification. In the present study, cationized P. eryngii polysaccharide (CPS), hybridized with calcium phosphate (CP), was used to codeliver plasmids (Oct4, Sox2, Klf4, c-Myc) for generating induced pluripotent stem cells (iPSCs). The results revealed that the hybrid nanoparticles could significantly enhance the process and efficiency of reprogramming (1.6-fold increase) compared with the CP nanoparticles. The hybrid CPS also facilitated epigenetic modification during the reprogramming. Moreover, these hybrid nanoparticles exhibited multiple pathways (both caveolae- and clathrin-mediated endocytosis) in their cellular internalization, which accounted for the improved iPSCs generation. These findings therefore present a novel application of P. eryngii polysaccharide in pluripotent reprogramming via active epigenetic modification.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Deng
authors.forename: Xia
authors.initials: X
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University , Nanjing 210009, People's Republic of China.
authors.forename: Qingtong
authors.initials: Q
authors.lastname: Yu
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Qu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.forename: Genbao
authors.initials: G
authors.lastname: Shao
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University , Nanjing 210009, People's Republic of China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Gao
authors.forename: Ximing
authors.initials: X
authors.lastname: Xu
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Yu
mesh_terms: D002454:Cell Differentiation; D065150:Cellular Reprogramming; D044127:Epigenesis, Genetic; D018014:Gene Transfer Techniques / Q000295:instrumentation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D011134:Polysaccharides
keywords: Pleurotus eryngii polysaccharide; calcium phosphate; hybrid nanoparticles; induced pluripotent stem cells; nonviral