title: The cellular internalization of liposome encapsulated protoporphyrin IX by HeLa cells.
abstract: The proper lipid composition of liposomes designed to carry drugs determines their surface properties ensuring their accumulation within selected tissue. The electrostatic potential and surface topology of liposomes affect the internalization by single cells. The high-resolution imaging of cancer cells and the distribution of protoporphyrin-loaded liposomes within the cytoplasm and its dependence on the liposome surface properties are presented. In the paper, HeLa cells were used to investigate the uptake of porphyrin-loaded liposomes and liposomes alone by means of confocal and differential interference contrast microscopies. The effect of liposomes surface electrostatic potential and surface topology on their intracellular distribution was evaluated. The time evolution of the intracellular distribution of liposomes labelled with Rhodamine-PE was examined on HeLa cells. These studies allow for the identification of the liposome lipid composition so the efficient delivery of the active substance to cancer cells will be achieved. The obtained results showed that neutral PC-liposomes are the most efficiently internalized by HeLa cells. Moreover, results showed that properties of liposomes affect not only the internalization efficiency of the photosensitizer but also its distribution within the cells, as revealed by colocalization measurements.
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Przybylo
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Glogocka
authors.affiliation: Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Jerzy W
authors.initials: JW
authors.lastname: Dobrucki
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland.
authors.forename: Kaja
authors.initials: K
authors.lastname: Fraczkowska
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Podbielska
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland. Electronic address: marta.kopaczynska@pwr.edu.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Kopaczynska
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Borowik
authors.affiliation: Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Technology, Wrocław, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Langner
mesh_terms: D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism; D011524:Protoporphyrins / Q000737:chemistry* / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D008055:Lipids; D008081:Liposomes; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D012235:Rhodamines; C028025:protoporphyrin IX
keywords: Cellular internalization; Colocalization; Confocal microscopy; Liposomes; Protoporphyrin IX