title: Direct and simultaneous quantification of ATP, ADP and AMP by (1)H and (31)P Nuclear Magnetic Resonance spectroscopy.
abstract: ATP, ADP and AMP are energy substances with vital biological significance. Based on the structural differences, a simple, rapid and comprehensive method has been established by (1)H and (31)P Nuclear Magnetic Resonance ((1)H-NMR and (31)P-NMR) spectroscopies. Sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TMSP) and anhydrous disodium hydrogen phosphate (Na2HPO4) were selected as internal standards for (1)H-NMR and (31)P-NMR, respectively. Those three compounds and corresponding internal standards can be easily distinguished both by (1)H-NMR and (31)P-NMR. In addition, they all have perfect linearity in a certain range: 0.1-100mM for (1)H-NMR and 1-75 mM for (31)P-NMR. To validate the precision of this method, mixed samples of different concentrations were measured. Recovery experiments were conducted in serum (91-113% by (1)H-NMR and 89-113% by (31)P-NMR).
authors.affiliation: Analytical & Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Yakun
authors.initials: Y
authors.lastname: Lian
authors.affiliation: Metabolomics and Multidisciplinary Laboratory for Trauma Research, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610064, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Metabolomics and Multidisciplinary Laboratory for Trauma Research, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610064, China.
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Feng
authors.affiliation: Analytical & Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Analytical & Testing Center, Sichuan University, Chengdu 610064, China; College of Chemistry, Sichuan University, Chengdu 610064, China.
authors.forename: Xiandeng
authors.initials: X
authors.lastname: Hou
authors.affiliation: Analytical & Testing Center, Sichuan University, Chengdu 610064, China. Electronic address: pcdeng@yahoo.com.
authors.forename: Pengchi
authors.initials: P
authors.lastname: Deng
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate
keywords: (31)P-NMR; ADP; AMP; ATP; Quantitative (1)H-NMR; Serum