title: Tissue nonautonomous effects of fat body methionine metabolism on imaginal disc repair in Drosophila.
abstract: Regulatory mechanisms for tissue repair and regeneration within damaged tissue have been extensively studied. However, the systemic regulation of tissue repair remains poorly understood. To elucidate tissue nonautonomous control of repair process, it is essential to induce local damage, independent of genetic manipulations in uninjured parts of the body. Herein, we develop a system in Drosophila for spatiotemporal tissue injury using a temperature-sensitive form of diphtheria toxin A domain driven by the Q system to study factors contributing to imaginal disc repair. Using this technique, we demonstrate that methionine metabolism in the fat body, a counterpart of mammalian liver and adipose tissue, supports the repair processes of wing discs. Local injury to wing discs decreases methionine and S-adenosylmethionine, whereas it increases S-adenosylhomocysteine in the fat body. Fat body-specific genetic manipulation of methionine metabolism results in defective disc repair but does not affect normal wing development. Our data indicate the contribution of tissue interactions to tissue repair in Drosophila, as local damage to wing discs influences fat body metabolism, and proper control of methionine metabolism in the fat body, in turn, affects wing regeneration.
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan;
authors.forename: Soshiro
authors.initials: S
authors.lastname: Kashio
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan; The Francis Crick Institute, London NW7 1AA, United Kingdom; fumiaki.obata@crick.ac.uk miura@mol.f.u-tokyo.ac.jp.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Obata
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario M5S 1A8, Canada;
authors.forename: Liu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan; Agency for Medical Research and Development-Core Research for Evolutional Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Katsuyama
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan; Agency for Medical Research and Development-Core Research for Evolutional Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Chihara
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan; Agency for Medical Research and Development-Core Research for Evolutional Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan fumiaki.obata@crick.ac.uk miura@mol.f.u-tokyo.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miura
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000502:physiology*; D005216:Fat Body / Q000378:metabolism*; D060227:Imaginal Discs / Q000502:physiology*; D008715:Methionine / Q000378:metabolism*; D012038:Regeneration; D013696:Temperature; D014921:Wings, Animal / Q000378:metabolism
chemical_list: D008715:Methionine
keywords: Drosophila melanogaster; genetic ablation; methionine metabolism; tissue nonautonomous; tissue repair
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