title: Differences in Anticipatory Behaviour between Rats (Rattus norvegicus) Housed in Standard versus Semi-Naturalistic Laboratory Environments.
abstract: Laboratory rats are usually kept in relatively small cages, but research has shown that they prefer larger and more complex environments. The physiological, neurological and health effects of standard laboratory housing are well established, but fewer studies have addressed the sustained emotional impact of a standard cage environment. One method of assessing affective states in animals is to look at the animals' anticipatory behaviour between the presentation of a cue signalling the arrival of a reward and the arrival of that reward. The primary aim of this study was to use anticipatory behaviour to assess the affective state experienced by female rats a) reared and housed long-term in a standard laboratory cage versus a semi-naturalistic environment, and b) before and after treatment with an antidepressant or an anxiolytic. A secondary aim was to add to the literature on anticipatory behaviour by describing and comparing the frequency and duration of individual elements of anticipatory behaviour displayed by rats reared in these two systems. In all experiments, total behavioural frequency was higher in standard-housed rats compared to rats from the semi-naturalistic condition, suggesting that standard-housed rats were more sensitive to rewards and experiencing poorer welfare than rats reared in the semi-naturalistic environment. What rats did in anticipation of the reward also differed between housing treatments, with standard-housed rats mostly rearing and rats from the semi-naturalistic condition mostly sitting facing the direction of the upcoming treat. Drug interventions had no effect on the quantity or form of anticipatory behaviour, suggesting that the poorer welfare experienced by standard-housed rats was not analogous to depression or anxiety, or alternatively that the drug interventions were ineffective. This study adds to mounting evidence that standard laboratory housing for rats compromises rat welfare, and provides further scientific support for recommendations that current minimum standards be raised.
authors.affiliation: Animal Welfare Program, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: I Joanna
authors.initials: IJ
authors.lastname: Makowska
authors.affiliation: Animal Welfare Program, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Weary
mesh_terms: D000818:Animals; D059031:Anticipation, Psychological; D001070:Appetitive Behavior; D004777:Environment; D005260:Female; D006799:Housing, Animal; D010865:Pilot Projects; D017207:Rats, Sprague-Dawley
references.citation: J Pharmacol Exp Ther. 2002 Jun;301(3):785-9
references.pmid: 12023504
references.citation: Neuroscience. 2012 Jun 28;213:72-80
references.pmid: 22521815
references.citation: Exp Brain Res. 2010 Apr;201(4):641-51
references.pmid: 19921157
references.citation: J Comp Physiol Psychol. 1977 Jun;91(3):649-61
references.pmid: 559696
references.citation: Behav Brain Res. 2006 Nov 25;175(1):1-8
references.pmid: 16970997
references.citation: Nat Rev Neurosci. 2000 Dec;1(3):191-8
references.pmid: 11257907
references.citation: Psychopharmacology (Berl). 1978 Jul 6;58(2):175-9
references.pmid: 98787
references.citation: FASEB J. 2001 Aug;15(10):1780-2
references.pmid: 11481228
references.citation: Nat Rev Neurosci. 2006 Sep;7(9):697-709
references.pmid: 16924259
references.citation: Behav Brain Res. 2003 Jun 16;142(1-2):151-6
references.pmid: 12798276
references.citation: Appl Anim Behav Sci. 2001 Apr 26;72(2):145-171
references.pmid: 11278033
references.citation: Behav Neurosci. 2013 Oct;127(5):712-24
references.pmid: 24128360
references.citation: J Comp Physiol Psychol. 1971 Oct;77(1):155-60
references.pmid: 5120680
references.citation: Dev Psychobiol. 1977 Mar;10(2):143-9
references.pmid: 838159
references.citation: PLoS One. 2012;7(7):e40642
references.pmid: 22815778
references.citation: Pharmacol Biochem Behav. 2006 Jul;84(3):517-23
references.pmid: 16899284
references.citation: J Dairy Sci. 2007 Jun;90 Suppl 1:E87-99
references.pmid: 17517755
references.citation: Psychopharmacology (Berl). 1996 Dec;128(4):331-42
references.pmid: 8986003
references.citation: Proc Natl Acad Sci U S A. 1917 Dec;3(12):685-8
references.pmid: 16586767
references.citation: Behav Brain Res. 2000 Dec 20;117(1-2):137-46
references.pmid: 11099767
references.citation: J Exp Anal Behav. 1991 Mar;55(2):245-50
references.pmid: 16812635
references.citation: Behaviour. 1967;28(3):280-306
references.pmid: 6038300
references.citation: Nature. 2004 Jan 22;427(6972):312
references.pmid: 14737158
references.citation: J Exp Anal Behav. 1998 Sep;70(2):103-21
references.pmid: 9841250
references.citation: Behav Brain Res. 1999 Dec;106(1-2):133-42
references.pmid: 10595429
references.citation: Psychopharmacology (Berl). 2002 Jul;162(3):232-8
references.pmid: 12122480
references.citation: Fundam Clin Pharmacol. 2009 Jun;23(3):323-30
references.pmid: 19453759
references.citation: Brain Res. 1990 Apr 23;514(1):22-6
references.pmid: 2357527
references.citation: Science. 2010 Aug 20;329(5994):913-4
references.pmid: 20724626
references.citation: J Comp Physiol Psychol. 1957 Dec;50(6):588-91
references.pmid: 13502503
references.citation: Lab Anim. 2001 Jan;35(1):74-9
references.pmid: 11201290
references.citation: Physiol Behav. 2006 Jun 15;88(1-2):132-7
references.pmid: 16678224
references.citation: Science. 2012 Oct 5;338(6103):68-72
references.pmid: 23042884
references.citation: Behav Processes. 2010 Oct;85(2):172-80
references.pmid: 20637270
references.citation: Eur J Pharmacol. 1976 Jan;35(1):45-58
references.pmid: 3421
references.citation: Neuropharmacology. 2013 Jul;70:27-34
references.pmid: 23337256
references.citation: Zoo Biol. 2014 Jul-Aug;33(4):251-6
references.pmid: 25042907
references.citation: Lab Anim. 2004 Oct;38(4):384-92
references.pmid: 15479553
references.citation: Behav Brain Res. 2005 Jun 20;161(2):183-9
references.pmid: 15922044
references.citation: Lab Anim. 2011 Jul;45(3):145-53
references.pmid: 21498639
references.citation: Biol Psychiatry. 2012 Oct 1;72(7):537-47
references.pmid: 22705040
references.citation: Psychopharmacology (Berl). 1995 Jan;117(1):116-24
references.pmid: 7724696
references.citation: Behav Brain Res. 2008 Nov 3;193(1):48-54
references.pmid: 18534693