title: A new in vitro mouse oligodendrocyte precursor cell migration assay reveals a role for integrin-linked kinase in cell motility.
abstract: BACKGROUND
  The decline of remyelination in chronic multiple sclerosis (MS) is in part attributed to inadequate oligodendrocyte precursor cell (OPC) migration, a process governed by the extracellular matrix (ECM). Elucidating the mechanisms underlying OPC migration is therefore an important step towards developing new therapeutic strategies to promote myelin repair. Many seminal OPC culture methods were established using rat-sourced cells, and these often need modification for use with mouse OPCs due to their sensitive nature. It is of interest to develop mouse OPC assays to leverage the abundant transgenic lines. To this end, we developed a new OPC migration method specifically suited for use with mouse-derived cells.
  
  
  RESULTS
  To validate its utility, we combined the new OPC migration assay with a conditional knockout approach to investigate the role of integrin-linked kinase (ILK) in OPC migration. ILK is a focal adhesion protein that stabilizes cellular adhesions to the extracellular matrix (ECM) by mediating a linkage between matrix-bound integrin receptors and the cytoskeleton. We identified ILK as a regulator of OPC migration on three permissive substrates. ILK loss produced an early, albeit transient, deficit in OPC migration on laminin matrix, while migration on fibronectin and polylysine was heavily reliant on ILK expression.
  
  
  CONCLUSIONS
  Inclusively, our work provides a new tool for studying mouse OPC migration and highlights the role of ILK in its regulation on ECM proteins relevant to MS.
authors.affiliation: Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada. ryanwomeara@gmail.com.|Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada. ryanwomeara@gmail.com.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: O'Meara
authors.affiliation: Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada. scumm013@uottawa.ca.|Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada. scumm013@uottawa.ca.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Cummings
authors.affiliation: Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada. jp.stan.michalski@gmail.com.|Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada. jp.stan.michalski@gmail.com.
authors.forename: John-Paul
authors.initials: JP
authors.lastname: Michalski
authors.affiliation: Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada. rkothary@ohri.ca.|Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada. rkothary@ohri.ca.|Department of Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada. rkothary@ohri.ca.|University of Ottawa Centre for Neuromuscular Disease, Ottawa, ON, K1H 8M5, Canada. rkothary@ohri.ca.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Kothary
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D002478:Cells, Cultured; D005353:Fibronectins / Q000502:physiology; D007797:Laminin / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009836:Oligodendroglia / Q000502:physiology*; D011107:Polylysine / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D000099200:Scaffold Protein ILK
chemical_list: D005353:Fibronectins; D007797:Laminin; D011107:Polylysine; D017346:Protein Serine-Threonine Kinases; D000099200:Scaffold Protein ILK
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