title: Effects of T cell immunoglobulin and mucin domain-containing molecule-3 signaling molecule on human monocyte-derived dendritic cells with hepatitis B virus surface antigen stimulation in vitro.
abstract: The aim of the present study was to investigate the in vitro effects of hepatitis B virus surface antigen (HBsAg) on the immune function of human monocyte-derived dendritic cells (MD‑DCs), and the moderating role of T cell immunoglobulin and mucin domain‑containing molecule‑3 (Tim‑3) signaling molecule. The monocytes, obtained from healthy adult peripheral blood, were incubated with recombinant human granulocyte‑macrophage colony‑stimulating factor and interleukin (IL)‑4 to induce DCs. DC‑associated cell markers were detected using flow cytometry. MD‑DCs were treated with HBsAg (5 µg/ml) in vitro for 48 h and subsequently, cell markers, lymphocyte stimulatory capacity, signaling protein and downstream cytokines were assessed. In addition, a Tim‑3 monoclonal antibody was used to inhibit the Tim‑3 signaling pathway, and subsequently the immune responses of MD‑DCs to HBsAg stimulation were determined using the aforementioned method. The cell phenotype expressions of MD‑DCs were all significantly increased with cluster of differentiation (CD)11c at 70.09±0.57%, human leukocyte antigen‑DR at 79.83±2.12%, CD80 at 48.33±7.34% and CD86 at 44.21±5.35%. The treatment of MD‑DCs with HBsAg resulted in a CD80 and CD86 enhanced expression, enhanced lymphocyte stimulatory capacity, upregulated expression of Tim‑3 and nuclear factor‑κB (NF‑κB), as well as enhanced cytokine secretion of IL‑6, IL‑10 and interferon (IFN)‑γ. However, a reduced immune response of MD‑DCs in response to HBsAg stimulation was observed when the Tim‑3 signaling pathway was inhibited prior to stimulation. The expression of NF‑κB was decreased and the cytokine secretion level of IL‑6, IL‑10 and IFN‑γ were downregulated. The treatment with HBsAg in vitro resulted in an enhanced immune response of MD‑DCs, which may be positively regulated by the Tim-3 signaling molecule.
authors.affiliation: Medical Research Center of Taizhou Hospital, Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Medical Research Center of Taizhou Hospital, Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Medical Research Center of Taizhou Hospital, Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Infectious Diseases, Affiliated Taizhou Hospital of Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China.
authors.forename: Kechuan
authors.initials: K
authors.lastname: Pan
authors.affiliation: Department of Infectious Diseases, Affiliated Taizhou Hospital of Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.affiliation: Department of Infectious Diseases, Affiliated Taizhou Hospital of Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Zhu
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D000072597:Hepatitis A Virus Cellular Receptor 2 / Q000502:physiology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D006514:Hepatitis B Surface Antigens; C508600:IL6 protein, human; D015850:Interleukin-6; D016328:NF-kappa B