title: Relapsed Melioidosis Model in C57BL/6 Mice.
abstract: BACKGROUND
  Burkholderia pseudomallei are the causative agents of melioidosis, a disease that has a high relapse rate in endemic areas. The mechanism of relapse is unclear
  
  
  OBJECTIVE
  This study aimed to establish relapsed melioidosis in C57BL/6 mice by induction with B. pseudomallei.
  
  
  METHODS
  Low doses of B. pseudomallei H777 and its biofilm defective mutant (M10) were intra-gastric fed to C57BL/6 mice. All the infected mice had suppressed immune status by intra-peritoneal injection of hydrocortisone at 2.5 mg per mouse at day 60 post-infection. Inflammatory response to the infection was investigated by histo-pathological studies and monitoring bacterial counts in the blood and organs.
  
  
  RESULTS
  All the infected mice were found to have a high infiltration of mononuclear cells at day 60 post-infection. The results showed high bacterial counts in the blood in both strains post-suppressed immune status after two days. The biofilm mutant and wild type strains produced relapse in C57BL/6 mice but the latter was responsible for significantly more severe inflammation than the biofilm mutant.
  
  
  CONCLUSIONS
  Low immune status may cause relapsed melioidosis in hosts with chronic inflammation.
authors.forename: Pawana
authors.initials: P
authors.lastname: Panomket
authors.forename: Parichart
authors.initials: P
authors.lastname: Wongsana
authors.forename: Surasak
authors.initials: S
authors.lastname: Wanram
authors.forename: Surasakdi
authors.initials: S
authors.lastname: Wongratanacheewin
authors.forename: Thanatchaporn
authors.initials: T
authors.lastname: Bartpho
mesh_terms: D000818:Animals; D016957:Burkholderia pseudomallei / Q000502:physiology*; D004195:Disease Models, Animal; D007113:Immunity, Innate; D007249:Inflammation / Q000276:immunology* / Q000382:microbiology; D008554:Melioidosis / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D012008:Recurrence; D013047:Specific Pathogen-Free Organisms