title: Evaluation of parameters obtained from the Sysmex XN-2000 for predicting the recovery of the absolute neutrophil count and platelets after hematopoietic stem cell transplantation.
abstract: BACKGROUND
  We analyzed abilities of parameters from Sysmex XN-2000 (Sysmex, Kobe, Japan) to predict absolute neutrophil count (ANC) and platelet recovery after hematopoietic stem cell transplantation (HSCT) in patients with hematologic malignancies.
  
  
  METHODS
  We prospectively analyzed 911 follow-up peripheral blood samples from 44 HSCT-performed patients and evaluated the performances of the following parameters: WBC, immature granulocyte (IG), hematopoietic stem and progenitor cells (HPC), immature reticulocyte fraction (IRF), immature platelet fraction (IPF), platelet distribution width (PDW), mean platelet volume (MPV), and platelet larger cell ratio (P-LCR).
  
  
  RESULTS
  When compared to four other parameters, the identification of initiation in IG (%)/HPC (%) increase enabled earlier prediction of ANC recovery to >500/μL and >1000/μL with more time benefit of 3.5-6.5 days/2.0-5.0 days and 3.0-6.0 days/2.0-5.0 days, respectively. When compared to IPF (%), the identification of initiation in PDW, MPV, and P-LCR (%) increase enabled earlier prediction of platelet recovery to >20 000/μL and >50 000/μL with more time benefit of 2.5-3.5 days and 2.0-3.0 days, respectively. However, the standard deviation of time benefit obtained from IG (%)/HPC (%)/PDW/MPV/P-LCR (%) was consistently large (3.0-4.3 days).
  
  
  CONCLUSIONS
  There is a systematic pattern where a rise in most of the studied parameters can be observed in most patients before ANC/platelet recovery. However, the interindividual variation between the time of rise of these parameters and ANC/platelet recovery is large, and therefore, using these parameters to predict recovery in the individual patient is probably not meaningful in the clinical setting.
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.|Department of Laboratory Medicine, Pusan National University School of Medicine, Biomedical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: C-J
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: M-J
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: M-Y
authors.initials: MY
authors.lastname: Han
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: B-R
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: Y-U
authors.initials: YU
authors.lastname: Cho
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: S-W
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Department of Hematology, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: D-Y
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Hematology, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: J-H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Hematology, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: J-H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Hematology, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
authors.forename: K-H
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000328:Adult; D005260:Female; D005500:Follow-Up Studies; D006085:Graft Survival; D019337:Hematologic Neoplasms / Q000097:blood / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils; D010976:Platelet Count; D011379:Prognosis; D011446:Prospective Studies; D015203:Reproducibility of Results; D013997:Time Factors; D055815:Young Adult
keywords: ANC; hematopoietic stem cell transplantation; parameter; platelets; recovery