title: Metabolic Effects of the pksCT Gene on Monascus aurantiacus Li As3.4384 Using Gas Chromatography--Time-of-Flight Mass Spectrometry-Based Metabolomics.
abstract: Monascus spp. have been used for the production of natural pigments and bioactive compounds in China for several centuries. Monascus can also produce the mycotoxin citrinin, restricting its use. Disruption of the pksCT gene in Monascus aurantiacus Li AS3.4384 reduces citrinin production capacity of this strain (Monascus PHDS26) by over 98%. However, it is unclear how other metabolites of M. aurantiacus Li AS3.4384 (the wild-type strain) are affected by the pksCT gene. Here, we used metabolomic analyses to compare red yeast rice (RYR) metabolite profiles of the wild-type strain and Monascus PHDS26 at different stages of solid-state fermentation. A total of 18 metabolites forming components within the glycolysis, acetyl-CoA, amino acid, and tricarboxylic acid (TCA) cycle metabolic processes were found to be altered between the wild-type strain and Monascus PHDS26 at different stages of solid-state fermentation. Thus, these findings provide important insights into the metabolic pathways affected by the pksCT gene in M. aurantiacus.
authors.affiliation: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, and ‡Center of Analysis and Testing, Nanchang University , 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, and ‡Center of Analysis and Testing, Nanchang University , 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, and ‡Center of Analysis and Testing, Nanchang University , 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, and ‡Center of Analysis and Testing, Nanchang University , 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.
authors.forename: Laisheng
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, and ‡Center of Analysis and Testing, Nanchang University , 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Li
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D055432:Metabolomics; D032741:Monascus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins
keywords: GC−TOF-MS; Monascus aurantiacus Li AS3.4384; metabolomics; pksCT gene disrupted strain