title: Bioaccumulation, oxidative stress and genotoxicity in fish (Channa punctatus) exposed to a thermal power plant effluent.
abstract: Metal bioaccumulation and induction of biomarkers such as lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione S transferase (GST), reduced glutathione (GSH) and DNA damage are potential indicators of stress in Channa punctatus exposed to effluents. In canal water, receiving thermal power plant discharges, Fe and Ni concentrations exceeded the recommended guidelines set by the United Nations Environment Programme Global Environment Monitoring System (UNEPGEMS). Fe was highly bioavailable and accumulated in all organs (liver, kidney, muscle and integument). The highest metal pollution index (MPI) value of 41.2 was observed in kidney and the lowest 13.5 in muscle tissue. LPO, SOD, CAT and GST levels were significantly higher in liver and kidney, whereas GSH levels declined significantly compared to fish from the reference site. Concomitant damage to DNA was observed with significantly higher mean tail length in the exposed fish gill cells (26.5µm) and in liver (20.8µm) compared to reference fish. Therefore, it can be concluded that the thermal power plant effluent had the potential to cause oxidative stress and DNA damage in C. punctatus.
authors.affiliation: Aquatic Toxicology Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India. Electronic address: mehjabeenjaved200@gmail.com.
authors.forename: Mehjbeen
authors.initials: M
authors.lastname: Javed
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
authors.forename: Irshad
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Aquatic Toxicology Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
authors.forename: Nazura
authors.initials: N
authors.lastname: Usmani
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
authors.forename: Masood
authors.initials: M
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage / Q000502:physiology*; D004784:Environmental Monitoring / Q000379:methods; D004787:Environmental Pollution / Q000009:adverse effects*; D005399:Fishes / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity*; D018384:Oxidative Stress / Q000502:physiology*; D011210:Power Plants; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D016166:Free Radical Scavengers; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: Bioaccumulation; Biomarker; Comet assay; Genotoxicity; Heavy metals; Oxidative stress