title: Association between Helicobacter pylori hopQI genotypes and human gastric cancer risk.
abstract: The Helicobacter pylori use a number of mechanisms to survive in the stomach lumen and can lead to gastritis and reduction in stomach acid secretion. It has been found that the risk of developing gastric carcinoma is associated to heterogeneity of H. pylori virulence factors such as HopQ. The HopQ is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also main role in the virulence of H. pylori. The purpose of the current study was to investigate the association between different H. pylori virulence hopQI (types I) genotyping and patients with gastroduodenal disorders. For this purpose 58 stomach biopsies of the patients with gastric cancer and 100 saliva samples from healthy and H. pylori infected individuals were collected and studied. Then genomic DNA was purified and PCR was done for desired gene via specific primers. The H. pylori infections were diagnosed using PCR for GlmM gene. Then frequencies of hopQI+ and hopQI- genotypes were determined in H. pylori infected cases. Statistical analysis showed that there were not significant differences between healthy and diseased ones for genotypes hopQI+ and hopQI-. Then the hopQI+ cannot be as a risk factor genotype for gastric cancer.
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: E
authors.initials: E
authors.lastname: Kazemi
authors.affiliation: Razi University Department of Agronomy and Plant Breeding, Faculty of Agriculture Kermanshah Iran dkahrizi@yahoo.com.
authors.forename: D
authors.initials: D
authors.lastname: Kahrizi
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: M T
authors.initials: MT
authors.lastname: Moradi
authors.affiliation: Razi University Zagros Bioidea Co., Razi University Incubator Kermanshah Iran.
authors.forename: M
authors.initials: M
authors.lastname: Sohrabi
authors.affiliation: Islamic Azad University Department of Biology, Sanandaj Branch Sanandaj Iran.
authors.forename: S
authors.initials: S
authors.lastname: Amini
authors.affiliation: The Scripps Research Institute Howard Hughes Medical Institute, Molecular and Cellular Neuroscience La Jolla USA.
authors.forename: S A R
authors.initials: SA
authors.lastname: Mousavi
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: K
authors.initials: K
authors.lastname: Yari
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D012306:Risk; D013274:Stomach Neoplasms / Q000209:etiology* / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D037521:Virulence Factors