title: Identification and molecular characterization of a new recombinant begomovirus and associated betasatellite DNA infecting Capsicum annuum in India.
abstract: Capsicum annuum (Chilli) is a perennial herbaceous plant that is cultivated as an annual crop throughout the world, including India. Chilli leaf curl disease (ChiLCD) is a major biotic constraint, causing major losses in chilli production. During 2014, leaf samples of chilli plants displaying leaf curl disease were collected from the Ahmedabad district of Gujarat, India. These samples were used to isolate, clone and sequence viral genomic DNA and an associated betasatellite DNA molecule. Sequence analysis showed 90.4 % nucleotide sequence identity to the previously reported chilli leaf curl virus-[India:Guntur:2009] (ChiLCV-[IN:Gun:09]. As per ICTV nomenclature rules, ChiLCV-Ahm represents a new species of begomovirus, and we therefore propose the name chilli leaf curl Ahmedabad virus-[India:Ahmedabad:2014] (ChiLCAV-[IN:Ahm:14]). The associated betasatellite DNA showed a maximum of 93.5 % nucleotide sequence identity to a previously reported tomato leaf curl Bangladesh betasatellite and may be named tomato leaf curl Bangladesh betasatellite-[India:Ahmedabad:Chilli:2014].
authors.affiliation: School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India.
authors.forename: Bhavin S
authors.initials: BS
authors.lastname: Bhatt
authors.affiliation: School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India.
authors.forename: Fenisha D
authors.initials: FD
authors.lastname: Chahwala
authors.affiliation: School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Rathod
authors.affiliation: School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India. achuits@gmail.com.
authors.forename: Achuit K
authors.initials: AK
authors.lastname: Singh
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics*; D002212:Capsicum / Q000821:virology*; D004276:DNA, Satellite / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016865:Reassortant Viruses / Q000235:genetics
chemical_list: D004276:DNA, Satellite; D004279:DNA, Viral