title: Characterizing optical properties and spatial heterogeneity of human ovarian tissue using spatial frequency domain imaging.
abstract: A spatial frequency domain imaging (SFDI) system was developed for characterizing ex vivo human ovarian tissue using wide-field absorption and scattering properties and their spatial heterogeneities. Based on the observed differences between absorption and scattering images of different ovarian tissue groups, six parameters were quantitatively extracted. These are the mean absorption and scattering, spatial heterogeneities of both absorption and scattering maps measured by a standard deviation, and a fitting error of a Gaussian model fitted to normalized mean Radon transform of the absorption and scattering maps. A logistic regression model was used for classification of malignant and normal ovarian tissues. A sensitivity of 95%, specificity of 100%, and area under the curve of 0.98 were obtained using six parameters extracted from the SFDI images. The preliminary results demonstrate the diagnostic potential of the SFDI method for quantitative characterization of wide-field optical properties and the spatial distribution heterogeneity of human ovarian tissue. SFDI could be an extremely robust and valuable tool for evaluation of the ovary and detection of neoplastic changes of ovarian cancer.
authors.affiliation: University of Connecticut, Biomedical Engineering Department, 371 Fairfield Way, U-4157, Storrs, Connecticut 06269-4157, United States.
authors.forename: Sreyankar
authors.initials: S
authors.lastname: Nandy
authors.affiliation: University of Connecticut, Department of Electrical and Computer Engineering, 371 Fairfield Way, U-4157, Storrs, Connecticut 06269-4157, United States.
authors.forename: Atahar
authors.initials: A
authors.lastname: Mostafa
authors.affiliation: University of Connecticut, Biomedical Engineering Department, 371 Fairfield Way, U-4157, Storrs, Connecticut 06269-4157, United States.
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Kumavor
authors.affiliation: University of Connecticut Health Center, Pathology Department, Farmington, Connecticut 06030, United States.
authors.forename: Melinda
authors.initials: M
authors.lastname: Sanders
authors.affiliation: University of Connecticut Health Center, Division of Gynecologic Oncology, Farmington, Connecticut 06030, United States.
authors.forename: Molly
authors.initials: M
authors.lastname: Brewer
authors.affiliation: University of Connecticut, Biomedical Engineering Department, 371 Fairfield Way, U-4157, Storrs, Connecticut 06269-4157, United StatesbUniversity of Connecticut, Department of Electrical and Computer Engineering, 371 Fairfield Way, U-4157, Storrs, Connect.
authors.forename: Quing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D016011:Normal Distribution; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000473:pathology; D010053:Ovary / Q000737:chemistry* / Q000473:pathology*; D019047:Phantoms, Imaging; D012542:Scattering, Radiation
references.citation: J Biomed Opt. 2012 Dec;17(12):126003
references.pmid: 23208214
references.citation: N Engl J Med. 2002 May 23;346(21):1609-15
references.pmid: 12023992
references.citation: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2015 Jan-Feb;7(1):1-16
references.pmid: 25329515
references.citation: Biomed Opt Express. 2013 Nov 06;4(12):2763-8
references.pmid: 24409378
references.citation: Exp Lung Res. 1995 Jan-Feb;21(1):67-77
references.pmid: 7537210
references.citation: PLoS Med. 2015 Feb;12(2):e1001789
references.pmid: 25710373
references.citation: Insights Imaging. 2012 Dec;3(6):573-89
references.pmid: 23093486
references.citation: Lancet Oncol. 2006 Oct;7(10):821-8
references.pmid: 17012044
references.citation: Transl Oncol. 2011 Feb 01;4(1):29-37
references.pmid: 21286375
references.citation: J Biomed Opt. 2010 Jul-Aug;15(4):046013
references.pmid: 20799815
references.citation: J Biomed Opt. 2014 Jul;19(7):071405
references.pmid: 24474551
references.citation: J Biomed Opt. 2011 Sep;16(9):090504
references.pmid: 21950907
references.citation: Breast Cancer Res. 2013;15(4):R61
references.pmid: 23915805
references.citation: N Engl J Med. 2002 May 23;346(21):1616-22
references.pmid: 12023993
references.citation: J Biomed Opt. 2013 Mar;18(3):036012
references.pmid: 23525360
references.citation: PLoS One. 2012;7(12):e51418
references.pmid: 23251526
references.citation: Opt Lett. 2005 Jun 1;30(11):1354-6
references.pmid: 15981531
references.citation: Lasers Surg Med. 2001;28(1):56-66
references.pmid: 11430444
references.citation: Lasers Surg Med. 1992;12(5):510-9
references.pmid: 1406004
references.citation: J Biomed Opt. 2009 Mar-Apr;14(2):024012
references.pmid: 19405742
references.citation: J Biomed Opt. 2012 Oct;17(10):107006
references.pmid: 23085984
references.citation: J Biomed Opt. 2014;19(7):070504
references.pmid: 25057960
references.citation: Ultrason Imaging. 2016 Jan;38(1):5-18
references.pmid: 25887527