title: Effect of prostaglandin reductase 1 (PTGR1) on gastric carcinoma using lentivirus-mediated system.
abstract: Gastric carcinoma is a digestive related malignant tumor with poor diagnosis and prognosis for advanced patients. PTGR1 (prostaglandin reductase 1), as a potential cancer biomarker, has not been reported in gastric carcinoma occurrence. To investigate the role of PTGR1 on gastric carcinoma cells, human PTGR1 was efficiently silenced by lentivirus-mediated system in MGC-803 cells confirmed by quantitative real-time PCR (qRT-PCR) and western blot. Then cell proliferation, colony formation and cell cycle were determined after knockdown of PTGR1 by MTT assay, colony assay and flow cytometry, respectively and data suggested that PTGR1 down regulated MGC-803 cells significantly suppressed the proliferation and colony formation ability and induced cell cycle arrest in the G0/G1 phase compared to controls (P < 0.001). Further investigation demonstrated knockdown of PTGR1 influenced cell proliferation and cell cycle via activating p21 and p53 signaling pathway described by Western blot assay. Our findings indicate that PTGR1 may be an oncogene in human gastric carcinoma and identified as a diagnosis and prognosis target for gastric carcinoma.
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Luo
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Affiliated Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Guo
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D015513:Oncogene Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D000429:Alcohol Oxidoreductases; C082646:leukotriene B4 12-hydroxydehydrogenase
keywords: Gastric carcinoma; RNA interference; cell cycle; prostaglandin reductase 1
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