title: Ancillary Studies, Including Immunohistochemistry and Molecular Studies, in Pancreatic Cytology.
abstract: Fine-needle aspiration biopsy of the pancreas is indicated for sampling of solid and cystic masses. Preoperative cytologic diagnosis of pancreatic ductal adenocarcinoma and cystic lesions on cytology can be problematic, and ancillary studies may help confirm diagnosis. Ancillary studies in pancreatic cytology include special stains, immunohistochemistry, mutational analyses of specific genes, cyst fluid analysis of tumor markers and enzymes, and, in some instances, flow cytometry. Proteomics, microRNA sequencing, and whole-exome gene sequencing have been used to illustrate the progression of pancreatic neoplasms and identify key diagnostic markers. This article summarizes recent literature on ancillary studies in pancreatic fine-needle aspiration samples.
authors.affiliation: Department of Pathology, Emory University Hospital, Room H189, 1364 Clifton Road, NE, Atlanta, GA 30322, USA.
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Reid
authors.affiliation: H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, MCC 2071H, Tampa, FL 33612, USA. Electronic address: Barbara.Centeno@moffitt.org.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Centeno
keywords: Ancillary studies; Fine-needle aspiration; Pancreas