title: Morphology and molecular analysis of Paratylenchus nanjingensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Pinus massoniana in China.
abstract: Paratylenchus nanjingensis n. sp. was obtained from Nanjing, Jiangsu Province, China. This new species is characterized by having a female with a slender, vermiform body (243-279 μm), head with distinct submedian lobes, slender and long stylet (64-68 μm), anchor-shaped stylet knobs, excretory pore anterior to the level of the stylet knobs, small lateral vulval flaps and lateral field with four lines; and male with more distinct body annuli, stylet lacking and pharynx degenerate. The internal transcribed spacer sequences of ribosomal RNA (ITS rRNA) gene of the new species were amplified and sequenced in this study. The phylogenetic relationships of the new species with other Paratylenchus species using the ITS rRNA gene sequences are given.
authors.affiliation: Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University,Guangzhou510642,China.
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University,Guangzhou510642,China.
authors.forename: H
authors.initials: H
authors.lastname: Xie
authors.affiliation: Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University,Guangzhou510642,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University,Guangzhou510642,China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University,Guangzhou510642,China.
authors.forename: C L
authors.initials: CL
authors.lastname: Xu
mesh_terms: D000818:Animals; D002681:China; D005260:Female; D008297:Male; D009348:Nematoda / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D028223:Pinus; D012987:Soil / Q000469:parasitology*
chemical_list: D012987:Soil