title: Effect of lipid extracts of Nigella sativa L. seeds on the liver ATP reduction and alpha-glucosidase inhibition.
abstract: Various extracts from the seeds of Nigella sativa have been used in traditional folk medicine to treat inflammation, liver disorders and arthritis. These seeds have been experimentally shown to possess antioxidant and hepatoprotective properties. Beside the hypoglycaemic and hypolipidemic effects, this study was carried out to evaluate, in vitro, toxicological effect of lipid extracts from the Nigella sativa seeds. The tested fractions were: (i) defatted methanolic extract, (ii) total lipid extract obtained by hexane extraction from methanolic extract and (iii) neutral and polar lipid fractions. The fractions were assessed, in vitro, for their inhibitory activity potential on the enzyme alpha-glucosidase as suppressing the enzyme activity is one among the therapeutic approaches to attenuate postprandial hyperglycemia. High inhibition of alpha-glucosidase by the two polar lipid fractions (F6 and F7) was reflected by their IC50 (0.51±0.04mg/ml and 0.55±0.09mg/ml, respectively), compared to acarbose (0.53±0.06mg/ml) and thymoquinone (0.65±0.05mg/ml). The hypoglycaemic effect of the polar lipid fraction of Nigella sativa could be explained by the inhibition of alpha-glucosidase, which is one of early steps of carbohydrate metabolism. Toxicological evaluation was investigated on precision-cut rat liver slices (PCLS). On PCLS, lipid extracts reduced ATP levels by 27 to 35%. Results indicate suggest that Nigella sativa extracts don't show a hepatoprotective effect against acetaminophen, but don't exhibit a major hepatotoxicity when tested alone.
authors.affiliation: Laboratory of Applied Biochemistry, Faculty of Life and Nature Sciences, University of Ferhat Abbas Setif 1, Algeria.
authors.forename: Widad
authors.initials: W
authors.lastname: Sobhi
authors.affiliation: Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Institute of Pharmacy, UniversitéLibre de Bruxelles, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Stevigny
authors.affiliation: Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Institute of Pharmacy, UniversitéLibre de Bruxelles, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Duez
authors.affiliation: Metabolism and Nutrition group (MNut), Louain Drug Research Institute, Catholic university of Louvain , 1200 Belgium.
authors.forename: Bedro Buc
authors.initials: BB
authors.lastname: Calderon
authors.affiliation: Department of Physical and Chemical Biology, Faculty of Life and Nature Sciences, University of Bejaia; Algeria.
authors.forename: Djebbar
authors.initials: D
authors.lastname: Atmani
authors.affiliation: Laboratory of Applied Biochemistry, Faculty of Life and Nature Sciences, University of Ferhat Abbas Setif 1, Algeria.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Benboubetra
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy; D005260:Female; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D031881:Nigella sativa; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds; D013311:Streptozocin; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D013311:Streptozocin; D000255:Adenosine Triphosphate; D000520:alpha-Glucosidases