title: Transposon Calling Cards.
abstract: Identifying the genomic targets of transcription factors is an important step in understanding the regulatory networks of gene transcription in yeast. We have developed a method that utilizes what we refer to as transposon "calling cards," in which a transcription factor directs the Ty5 retrotransposase to insert transposons into the genome adjacent to where the transcription factor binds. This method is designed to be multiplexed with many barcoded transcription factors and has the potential to decrease the labor required for the study of large numbers of transcription factors.
authors.affiliation: Department of Genetics, Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: David
authors.initials: D
authors.lastname: Mayhew
authors.affiliation: Department of Genetics, Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: Robi D
authors.initials: RD
authors.lastname: Mitra
mesh_terms: D001665:Binding Sites; D002874:Chromosome Mapping / Q000379:methods; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000378:metabolism; D053263:Gene Regulatory Networks; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D014157:Transcription Factors