title: Transcriptome sequencing and characterization of ungerminated and germinated spores of Nosema bombycis.
abstract: Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect Bombyx mori. Germination, an indispensible process through which microsporidia infect the host cells, is regarded as a key developmental turning point for microsporidia from dormant state to reproduction state. Thus, elucidating the transcriptome changes before and after germination is crucial for parasite control. However, the molecular basis of germination of microsporidia remains unknown. To investigate this germination process, the transcriptome of N. bombycis ungerminated spores and germinated spores were sequenced and analyzed. More than 60 million high-quality transcript reads were generated from these two groups using RNA-Seq technology. After assembly, 2756 and 2690 unigenes were identified, respectively, and subsequently annotated based on known proteins. After analysis of differentially expressed genes, 66 genes were identified to be differentially expressed (P ≤ 0.05) between these two groups. A protein phosphatase-associated gene was first identified to be significantly up-regulated as determined by RNA-Seq and immunoblot analysis, indicating that dephosphorylation might potentially contribute to microsporidia germination. The DEGs that encode proteins involved in glycometabolism, spore wall proteins and ricin B lectin of N. bombycis were also analyzed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed genes responsible for some specific biological functions and processes. The datasets generated in this study provide a basic characterization of the transcriptome changes in N. bombycis during germination. The analysis of transcriptome data and identification of certain functional genes which are robust candidate genes related to germination will help to provide a deep understanding of spore germination and invasion.
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Tongde Hospital of Zhejiang Province, Hangzhou 310058, China.
authors.forename: Mingqian
authors.initials: M
authors.lastname: Li
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: He
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shunfeng
authors.initials: S
authors.lastname: Cai
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiangkang
authors.initials: X
authors.lastname: He
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China xmlu@zju.edu.cn.
authors.forename: Xingmeng
authors.initials: X
authors.lastname: Lu
mesh_terms: D005800:Genes, Fungal; D018525:Germination; D016818:Nosema / Q000235:genetics / Q000502:physiology*; D013172:Spores, Fungal; D059467:Transcriptome
keywords: Nosema bombycis; dephosphorylation; germination; microsporidia; transcriptome
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