title: Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.
abstract: To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP) using the clustered regularly interspaced short palindromic repeats (CRISPR) system or transcription activator-like (TAL) proteins. Essentially, a locus-specific ChIP consists of locus-tagging and affinity purification and can be combined with downstream analyses to identify molecules associated with the target genomic regions. In this review, we discuss the applications of locus-specific ChIP to analyze the genome functions, including transcription and epigenetic regulation.
authors.affiliation: Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Fujita
authors.affiliation: Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
authors.forename: Hodaka
authors.initials: H
authors.lastname: Fujii
keywords: CRISPR; ChIP; TAL; chromatin immunoprecipitation; enChIP; engineered DNA-binding molecule-mediated ChIP; iChIP; insertional ChIP; locus-specific ChIP
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