title: MiR-205 suppresses autophagy and enhances radiosensitivity of prostate cancer cells by targeting TP53INP1.
abstract: OBJECTIVE
  This study aimed to investigate the role of miR-205 in radiosensitivity and autophagy of prostate cancer cells and to explore its regulative effect on TP53INP1.
  
  
  METHODS
  MiR-205 expression was compared in three prostate cancer cell lines (DU145, PC-3 and LNCaP) and one normal human prostate epithelial cell line (RWPE-1). The effect of irradiation-induced autophagy on radiosensitivity of the cancer cells and the effect of miR-205 on irradiation-induced autophagy were explored. The regulative effect of miR-205 on TP53INP1 and the function of this axis was further studied.
  
  
  RESULTS
  Ectopic expression of miR-205 substantially reduced the survival fraction of both DU145 and LNCaP cells to irradiation and inhibited irradiation-induced autophagy. Irradiation-induced autophagy acted as a protective mechanism in prostate cancer cells. TP53INP1 is a direct functional target of miR-205 in irradiation-induced autophagy and radiosensitivity regulation.
  
  
  CONCLUSIONS
  The miR-205/TP53INP1 mediated autophagy pathway might be an important molecular mechanism regulating radiosensitivity of prostate cancer cells and represents a potential therapeutic target for prostate cancer.
authors.affiliation: Department of Radiation Oncology, Henan Provincial People's Hospital, ZhengZhou, China. weiwang1980s@tom.com.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
mesh_terms: D001343:Autophagy / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D035683:MicroRNAs; C534425:MIR205, human; C429767:TP53INP1 protein, human