title: Association between EGFR polymorphisms and the risk of lung cancer.
abstract: OBJECTIVE
  The study aimed to investigate the role of epidermal growth factor receptor (EGFR) rs6965469 and rs763317 polymorphisms in the occurrence and development of lung cancer.
  
  
  METHODS
  We used polymerase chain reaction-ligation detection reaction (PCR-LDR) method to detect the genotypes of EGFR rs6965469 and rs763317 polymorphisms and the data were analyzed by GeneMapper software. Odds ratios (ORs) with 95% confidence intervals (CIs) was calculated by χ(2) test to estimate the significance difference of genotype and allele frequencies in case and control groups. ORs and 95% CIs were adjusted by logistic regression analysis with age, gender, drinking and smoking. The genotypes distributions of control group were tested by Hardy-Weinberg equilibrium (HWE).
  
  
  RESULTS
  The genotypes frequencies of controls for rs6965469 and rs763317 polymorphims were consistent with HWE. The distribution of rs6965469 TT genotype in two groups was significantly different (P<0.05) and TT genotype was associated with an increased risk of lung cancer (OR=6.92, 95% CI=1.33-36.00). AA genotype and A allele of rs763317 were also the susceptible factors of lung cancer. Individuals with AA genotype or A allele were more likely to suffer lung cancer (AA vs. GG: OR=7.20, 95% CI=1.33-39.07; A vs. G: OR=2.61, 95% CI=1.04-6.59).
  
  
  CONCLUSIONS
  The EGFR rs6965469 and rs763317 polymorphisms may be risk factors for lung cancer.
authors.affiliation: Department of Respiratory Medicine, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Respiratory Medicine, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Yufa
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016015:Logistic Models; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; lung cancer; polymorphisms
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