title: Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina.
abstract: The identification and characterisation of Cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity of Cryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays for Cryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Regina Helena Saramago
authors.initials: RH
authors.lastname: Peralta
authors.affiliation: Hospital Municipal de Infecciosas Dr Francisco Javier Muñiz, Buenos Aires, Argentina.
authors.forename: Jorge Néstor
authors.initials: JN
authors.lastname: Velásquez
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Flavia de Souza
authors.initials: Fde S
authors.lastname: Cunha
authors.affiliation: Administración Nacional de Laboratorios e Institutos de Salud Dr Carlos G Malbrán, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Pantano
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brasil.
authors.forename: Fernando Campos
authors.initials: FC
authors.lastname: Sodré
authors.affiliation: Laboratório de Parasitologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Sidnei da
authors.initials: Sd
authors.lastname: Silva
authors.affiliation: Administración Nacional de Laboratorios e Institutos de Salud Dr Carlos G Malbrán, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
authors.forename: Osvaldo Germán
authors.initials: OG
authors.lastname: Astudillo
authors.affiliation: Instituto de Microbiologia Prof Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Peralta
authors.affiliation: Administración Nacional de Laboratorios e Institutos de Salud Dr Carlos G Malbrán, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
authors.forename: Silvana
authors.initials: S
authors.lastname: Carnevale
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001118:Argentina; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000382:microbiology*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
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