title: The Effect of Platelet-Rich-Plasma on the Healing of Left Colonic Anastomosis in a Rat Model of Intra-Abdominal Sepsis.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of platelet-rich plasma (PRP) on the healing of colonic anastomosis in the presence of sepsis.
  
  
  METHODS
  Fifty Wistar-albino male rats were used. Ten healthy rats were euthanized to prepare PRP, the rest were subjected to colonic anastomosis and randomly allocated into four groups of 10 rats each as anastomosis without PRP (C), without PRP in sepsis (SC), anastomosis with PRP (C-PRP), and with PRP in sepsis (S-PRP). Sepsis was induced by cecal ligation and puncture procedure. All animals were euthanized on postoperative day 7. The body weight change, anastomotic bursting pressure (ABP), tissue hydroxyproline (TH) and histopathological examination of each group were analyzed by using one-way analysis of variance (ANOWA) and Tukey's HSD post-hoc test to assess the differences between the groups.
  
  
  RESULTS
  There was no statistical difference among the groups in terms of body weight changes. The ABP was measured at a mean value of 179.5 ± 10.3, 129.3 ± 14.2, 209 ± 14.4, and 167.5 ± 7.5 mm-Hg, in group C, SC, C-PRP, and S-PRP, respectively. The ABP and TH of C-PRP group was significantly higher than three groups (p < .05, for each comparison). In sepsis, PRP significantly raised the mean ABP and TH levels up to the levels of C group. Tissue regeneration was significant with increased collagen formation in C-PRP group than the other groups (p < .05). The healing effect of PRP in the presence of sepsis was significant than S-group (p < .05), while similar to C group (p = .181).
  
  
  CONCLUSIONS
  PRP application to colonic anastomosis promotes the healing process in rats with intra-abdominal sepsis.
authors.affiliation: a Department of General Surgery , Adana Numune Training and Research Hospital , Adana , Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Sozutek
authors.affiliation: b Department of General Surgery , Mersin University Medical Faculty , Mersin , Turkey.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Colak
authors.affiliation: a Department of General Surgery , Adana Numune Training and Research Hospital , Adana , Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Cetinkunar
authors.affiliation: a Department of General Surgery , Adana Numune Training and Research Hospital , Adana , Turkey.
authors.forename: Enver
authors.initials: E
authors.lastname: Reyhan
authors.affiliation: a Department of General Surgery , Adana Numune Training and Research Hospital , Adana , Turkey.
authors.forename: Oktay
authors.initials: O
authors.lastname: Irkorucu
authors.affiliation: c Department of Biochemistry , Mersin University Medical Faculty , Mersin , Turkey.
authors.forename: Gurbuz
authors.initials: G
authors.lastname: Polat
authors.affiliation: d Department of Pathology , Adana Numune Training and Research Hospital , Adana , Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Cennet
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D006133:Growth Substances / Q000502:physiology; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D053657:Platelet-Rich Plasma / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000473:pathology / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D006133:Growth Substances; D006909:Hydroxyproline
keywords: Platelet-rich-plasma; colonic anastomosis; intra-abdominal sepsis; surgery