title: Basic Histopathological Methods and Breast Lesion Types for Research.
abstract: The in situ observation on the tissues, such as histopathology, immunohistochemistry (IHC), immunofluorescence (IF), and in situ hybridization (ISH), is one of the most important methods in the biomedical scientific research. In this chapter we introduce the most often used methods-hematoxylin and eosin (H&E) and double IF staining. H&E staining is used for general morphology by which the different pathological types of breast lesions are identified. The double IF staining is often used to study the protein-protein interaction on tissues for signaling mechanisms. This chapter also includes the histopathology of primary or simplified breast lesion types that is essential for applying the above methods and the reclassification of breast cancers by molecular markers.
authors.affiliation: Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 33 Yingfeng Road, Guangzhou, 510228, People's Republic of China. ouynt@mail.sysu.edu.cn.
authors.forename: Nengtai
authors.initials: N
authors.lastname: Ouyang
authors.affiliation: Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 33 Yingfeng Road, Guangzhou, 510228, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D013194:Staining and Labeling
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin
keywords: Breast cancer; Hematoxylin & eosin; Histopathology; Immunofluorescence