title: Immobilization of Stimuli-Responsive Nanogels onto Honeycomb Porous Surfaces and Controlled Release of Proteins.
abstract: In this article, we describe the formation of functional honeycomb-like porous surfaces fabricated by the breath figures technique using blends of either amino-terminated poly(styrene) or a poly(styrene)-b-poly(acrylic acid) block copolymer with homopoly(styrene). Thus, the porous interfaces exhibited either amino or acid groups selectively located inside of the holes, which were subsequently employed to anchor stimuli-responsive nanogels by electrostatic interactions. These nanogels were prepared from poly(N-isopropylacrylamide) (PNIPAM) cross-linked with dendritic polyglycerol (dPG) and semi-interpenetrated with either 2-(dimethylamino)ethyl methacrylate (DMAEMA) or 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) to produce positively and negatively charged nanogel surfaces, respectively. The immobilization of these semi-interpenetrated networks onto the surfaces allowed us to have unique stimuli-responsive surfaces with both controlled topography and composition. More interestingly, the surfaces exhibited stimuli-responsive behavior by variations on the pH or temperature. Finally, the surfaces were evaluated regarding their capacity to induce a thermally triggered protein release at temperatures above the cloud point temperature (T(cp)) of the nanogels.
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (ICTP), Consejo Superior de Investigaciones Científicas (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: A S
authors.initials: AS
authors.lastname: De León
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin , Takustr. 3, 14195 Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Molina
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin , Takustr. 3, 14195 Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wedepohl
authors.affiliation: Departamento de Química Física Aplicada, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Cantoblanco, 28049 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Muñoz-Bonilla
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (ICTP), Consejo Superior de Investigaciones Científicas (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez-Hernández
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin , Takustr. 3, 14195 Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Calderón
mesh_terms: D000818:Animals; D002417:Cattle; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005782:Gels; D011108:Polymers; D012710:Serum Albumin, Bovine