title: Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cells.
abstract: This study is aimed to investigate the differentially expressed long non-coding RNAs (lncRNAs) in colorectal cancer and its potential biological function. Colorectal adenoma is the precancerous lesions of colorectal cancer, so in this study, we used colorectal adenoma as negative control. The global lncRNA expression profile in colorectal cancer and adenoma was evaluated by bioinformatics. The biological functions and potential mechanism of AK027294 were investigated in HCT116, HCT8, and SW480 colorectal cancer cells. A total of 135 lncRNAs were found to be differentially expressed in colorectal cancer and adenoma tissues. Among them, 71 lncRNAs were up-regulated and 64 lncRNAs were down-regulated. Especially, AK027294 was found to be highly expressed in colorectal cancer tissues compared with colorectal adenoma tissues (fold change is 184.5). Our results indicated that AK027294 down-regulation significantly inhibited colorectal cancer cells proliferation and migration, but promoted cell apoptosis (P < 0.05). The potential mechanism of AK027294 might be associated with the regulation of caspase-3, caspase-8, Bcl-2, MMP12, MMP9, and TWIST. The lncRNA expression profile in colorectal cancer suggests lncRNAs may play important roles in the occurrence and progression of colorectal cancer. AK027294 is highly expressed in colorectal cancer and closely correlates with colorectal cells proliferation, migration, and apoptosis.
authors.affiliation: Academy of Military Medical Sciences, Beijing, 100850, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Niu
authors.affiliation: Tumor Research Institute, Hangzhou Cancer Hospital, Hangzhou, 310002, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Oncology, Hainan Branch of PLA General Hospital, Sanya, 572013, China. woshiliuhui1995@aliyun.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Third Healthcare Division, Hainan Branch of Chinese PLA General Hospital, Sanya, 572013, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Department of Oncology, General Hospital of Chinese PLA, Beijing, 100853, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Tumor Research Institute, Hangzhou Cancer Hospital, Hangzhou, 310002, China. wushixiu2000@sina.com.
authors.forename: Shixiu
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Oncology, General Hospital of Chinese PLA, Beijing, 100853, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000236:Adenoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis; D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004261:DNA Replication; D020869:Gene Expression Profiling; D006801:Humans; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000502:physiology; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D046888:Tissue Array Analysis; D014162:Transfection; D014945:Wound Healing
chemical_list: D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; C000614198:long non-coding RNA AK027294, human
keywords: AK027294; Apoptosis; Colorectal cancer; Long non-coding RNAs; Migration; Proliferation
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