title: Interspecies chimeric complementation for the generation of functional human tissues and organs in large animal hosts.
abstract: The past decade's rapid progress in human pluripotent stem cell (hPSC) research has generated hope for meeting the rising demand of organ donation, which remains the only effective cure for end-stage organ failure, a major cause of death worldwide. Despite the potential, generation of transplantable organs from hPSCs using in vitro differentiation is far-fetched. An in vivo interspecies chimeric complementation strategy relying on chimeric-competent hPSCs and zygote genome editing provides an auspicious alternative for providing unlimited organ source for transplantation.
authors.affiliation: Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA, 92037, USA. Junwu@salk.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA, 92037, USA. Belmonte@salk.edu.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D001755:Blastocyst; D002454:Cell Differentiation / Q000235:genetics; D002678:Chimera / Q000235:genetics*; D005818:Genetic Engineering / Q000639:trends*; D006801:Humans; D016377:Organ Transplantation / Q000639:trends*; D039904:Pluripotent Stem Cells / Q000637:transplantation; D057905:Stem Cell Research; D014183:Transplantation, Heterologous / Q000639:trends
keywords: Blastocyst complementation; In vivo genome editing; Interspecies chimera; Organ transplantation; Pluripotent stem cells; iPSCs
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