title: Loss of nuclear localization of TET2 in colorectal cancer.
abstract: 5-Hydroxymethylcytosine (5hmC) is lost in multiple human cancers, including colorectal cancer (CRC). Decreased ten-eleven translocation 1 (TET1) messenger RNA (mRNA), but not other two TET family members, has been observed in the colorectal cancer and is crucial for colorectal cancer initiation. Here, we show that nuclear localization of TET2 was lost in a significant portion of CRC tissues, in association with metastasis. In CRC cells, nuclear expression of TET2 were absent but not TET3. Nuclear export inhibitor can increase the 5hmC level in CRC cells, probably through regulating TET2. Our results indicate a new mechanism of TET2 dysregulation in colorectal cancer.
authors.affiliation: Department of colorectal and anal surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Shanghai Colorectal Cancer Research Centre, Shanghai, China.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of colorectal and anal surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Shanghai Colorectal Cancer Research Centre, Shanghai, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of colorectal and anal surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Shanghai Colorectal Cancer Research Centre, Shanghai, China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of colorectal and anal surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Shanghai Colorectal Cancer Research Centre, Shanghai, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of colorectal and anal surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Shanghai Colorectal Cancer Research Centre, Shanghai, China.
authors.forename: Long
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of colorectal and anal surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Shanghai Colorectal Cancer Research Centre, Shanghai, China.
authors.forename: Chen-Ying
authors.initials: CY
authors.lastname: Liu
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000209:etiology / Q000235:genetics*; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000235:genetics*; D049308:Dioxygenases; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006801:Humans; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D005231:Fatty Acids, Unsaturated; D011518:Proto-Oncogene Proteins; D049308:Dioxygenases; C541081:TET2 protein, human; C038753:leptomycin B
keywords: 5hmC; Colorectal cancer; DNA methylation; Nuclear localization; TET2
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