title: Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-Aegilops mutica Partial Amphiploid and Addition Line.
abstract: Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA)8 as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of Ae. mutica chromosomes, with both wheat 7B chromosomes missing. EST-based molecular marker data suggested that the wheat-Ae. mutica addition line carries the 7T chromosome. Resistance tests indicated that both the partial amphiploid and the 7T addition line were highly resistant to powdery mildew, whereas the wheat control line Chinese Spring was highly susceptible, indicating the presence of a potentially new powdery mildew resistance gene on the Ae. mutica 7T chromosome. The karyotype, FISH patterns, and molecular markers can now be used to identify Ae. mutica chromatin in a wheat background, and the 7T addition could be used as a new powdery mildew resistance source for wheat breeding.
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Guang-Rong
authors.initials: GR
authors.lastname: Li
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Gong
authors.forename: Gen-Ying
authors.initials: GY
authors.lastname: Li
authors.forename: Ran
authors.initials: R
authors.lastname: Han
authors.forename: Hao-Sheng
authors.initials: HS
authors.lastname: Li
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Song
authors.forename: Ai-Feng
authors.initials: AF
authors.lastname: Liu
authors.forename: Xin-You
authors.initials: XY
authors.lastname: Cao
authors.forename: Xiu-Sheng
authors.initials: XS
authors.lastname: Chu
authors.forename: Zu-Jun
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Cheng-Yan
authors.initials: CY
authors.lastname: Huang
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Zhao
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000502:physiology; D002871:Chromosome Banding; D020732:Cytogenetic Analysis / Q000379:methods*; D004171:Diploidy; D060467:Disease Resistance / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D007621:Karyotyping; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D011123:Polyploidy; D013045:Species Specificity; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000382:microbiology