title: Matrine inhibited proliferation and increased apoptosis in human breast cancer MCF-7 cells via upregulation of Bax and downregulation of Bcl-2.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effects of matrine on proliferation and apoptosis in human breast cancer MCF-7 cells and its relevant molecular mechanisms.
  
  
  METHODS
  Breast carcinoma cell line MCF-7 was cultured with series concentrations of Matrine in vitro. The proliferation and apoptosis of MCF-7 cells were investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and Mitochondrial membrane potential (MMP) measurements. The expression levels of Bax and Bcl-2 proteins were detected by Annexin V/propidium iodide coupled staining. The morphological changes of MCF-7 cell were examined.
  
  
  RESULTS
  The inhibition rates of MCF-7 cells were 6.01%-37.01%, 7.56%-53.92%, and 10.86%-70.23% for 24, 48, and 72 hours after Matrine treatment, respectively. The proliferation of MCF-7 cells was significantly inhibited by Matrine administration, with a time and dose dependent manner. The rates apoptotic cells was between 4.17±0.25% and 19.63±0.17% in 0.25-2.0 mg/ml Matrine groups, which had significant increased compare with the control groups (1.10±0.08%, P<0.05). Meanwhile, increased Bax expression, but decreased Bcl-2 expression was observed in MCF-7 cell line. MMP were significantly decreased by Matrine treatment.
  
  
  CONCLUSIONS
  Matrine significantly inhibited the growth and induced apoptosis in breast carcinoma MCF-7 cells, which is related to Bax, Bcl-2 signaling and MMP.
authors.affiliation: Department of Pathology, Hebei North University China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pathology, Hebei North University China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pathology, Hebei North University China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Bai
authors.affiliation: Department of Gastric and Colorectal Surgery, First Hospital of Jilin University China.
authors.forename: Yueer
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Department of Pathology, Hebei North University China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Gastric and Colorectal Surgery, First Hospital of Jilin University China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Cao
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D061986:MCF-7 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D011807:Quinolizines / Q000494:pharmacology*; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D011807:Quinolizines; D051028:bcl-2-Associated X Protein; D000093842:Matrines
keywords: Bax; Bcl-2; Matrine; apoptosis; breast cancer; mitochondrial; proliferation
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