title: Proteomics of Secretory-Stage and Maturation-Stage Enamel of Genetically Distinct Mice.
abstract: The mechanisms by which excessive ingestion of fluoride (F) during amelogenesis leads to dental fluorosis (DF) are still not precisely known. Inbred strains of mice vary in their susceptibility to develop DF, and therefore permit the investigation of underlying molecular events influencing DF severity. We employed a proteomic approach to characterize and evaluate changes in protein expression from secretory-stage and maturation-stage enamel in 2 strains of mice with different susceptibilities to DF (A/J, i.e. 'susceptible' and 129P3/J, i.e. 'resistant'). Weanling male and female susceptible and resistant mice fed a low-F diet were divided into 2 F-water treatment groups. They received water containing 0 (control) or 50 mg F/l for 6 weeks. Plasma and incisor enamel was analyzed for F content. For proteomic analysis, the enamel proteins extracted for each group were separated by 2-dimensional electrophoresis and subsequently characterized by liquid-chromatography electrospray-ionization quadrupole time-of-flight mass spectrometry. F data were analyzed by 2-way ANOVA and Bonferroni's test (p < 0.05). Resistant mice had significantly higher plasma and enamel F concentrations when compared with susceptible mice in the F-treated groups. The proteomic results for mice treated with 0 mg F/l revealed that during the secretory stage, resistant mice had a higher abundance of proteins than their susceptible counterparts, but this was reversed during the maturation stage. Treatment with F greatly increased the number of protein spots detected in both stages. Many proteins not previously described in enamel (e.g. type 1 collagen) as well as some uncharacterized proteins were identified. Our findings reveal new insights regarding amelogenesis and how genetic background and F affect this process.
authors.affiliation: Department of Biological Sciences, Bauru Dental School, University of Sx00E3;o Paulo, Bauru, Brazil.
authors.forename: Senda
authors.initials: S
authors.lastname: Charone
authors.forename: Aline
authors.initials: A
authors.lastname: De Lima Leite
authors.forename: Camila
authors.initials: C
authors.lastname: Peres-Buzalaf
authors.forename: Mileni
authors.initials: M
authors.lastname: Silva Fernandes
authors.forename: Lucas
authors.initials: L
authors.lastname: Ferreira de Almeida
authors.forename: Marcia Sirlene
authors.initials: MS
authors.lastname: Zardin Graeff
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Cardoso de Oliveira
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
authors.forename: Sonia
authors.initials: S
authors.lastname: Groisman
authors.forename: Gary Milton
authors.initials: GM
authors.lastname: Whitford
authors.forename: Eric T
authors.initials: ET
authors.lastname: Everett
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000566:Amelogenesis; D000818:Animals; D003743:Dental Enamel; D005260:Female; D009050:Fluorosis, Dental; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D040901:Proteomics