title: [Effects of paclitaxel loaded-drug micelles on cell proliferation and apoptosis of human lung cancer A549 cells].
abstract: This study was conducted to investigate the paclitaxel loaded by hydrazone bonds in poly(ethylene glycol)-poly(caprolactone) micelles (mPEG-PCL-PTX) on proliferation and apoptosis of human lung cancer A549 cells and its possible mechanisms of anti-tumor activity. The cell proliferation was measured with MTT assay. Flow cytometry were used to analyze the cell cycle. The cell apoptosis was analyzed using Hoechst/P staining. The expression levels of apoptotic genes expression in the mitochondrial apoptosis pathway were detected by RT-PCR and Western blotting, respectively. The mPEG-PCL-PTX could inhibit the proliferation of A549 cells and promote the apoptosis. The Bax, caspase-3 protein expression were increased while Bcl-2 protein expression was decreased in A549 cells. Results showed that the polymer containing hydrazone bond is non-toxic in vitro, the mPEG-PCL-PTX micelles can inhibit the proliferation and induce the apoptosis of A549 cells. Key words: paclitaxel; micelle; A549 cell; proliferation; cell cycle; apoptosis
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Rui-shuang
authors.initials: RS
authors.lastname: Yu
authors.forename: Wen-liang
authors.initials: WL
authors.lastname: Yang
authors.forename: Shu-juan
authors.initials: SJ
authors.lastname: Luan
authors.forename: Ben-kai
authors.initials: BK
authors.lastname: Qin
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Pang
authors.forename: Guan-hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008823:Micelles; D017239:Paclitaxel / Q000494:pharmacology*; D011091:Polyesters; D011092:Polyethylene Glycols; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D008823:Micelles; D011091:Polyesters; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C439611:methoxy poly(ethylene glycol-co-epsilon-caprolactone); D011092:Polyethylene Glycols; C505407:CASP3 protein, human; D053148:Caspase 3; D017239:Paclitaxel