title: Andrographolide Ameliorates Beta-Naphthoflavone-Induced CYP1A Enzyme Activity and Lipid Peroxidation in Hamsters with Acute Opisthorchiasis.
abstract: BACKGROUND
  Opisthorchis viverrini (OV) infection generates oxidative stress/free radicals and is considered as a primary cause ofcholangiocarcinoma since it primarily triggers sclerosing cholangitis.
  
  
  OBJECTIVE
  In this study, the impacts of andrographolide on acute opisthorchaisis in β-naphthoflavone (BNF)-exposed hamsters were investigated.
  
  
  METHODS
  Ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) activities and Thiobarbituric acid reaction substances (TBARS) assay of andrographolide in acute opisthorchiasis in the BNF-exposed hamsters were assessed.
  
  
  RESULTS
  The results showed that andrographolide ameliorated the hepatic CYP1A1 and CYP1A2 activities by decreases of the specific enzymatic reactions of EROD and MROD, respectively, in the BNF-exposed hamsters. Moreover, andrographolide lowered the formation of malondialdehyde in the livers and brains of the hamsters.
  
  
  CONCLUSIONS
  These observations revealed the promising chemo-protective and antioxidant activities of andrographolide via suppression of the specific EROD and MROD reactions and lipid peroxidation against acute opisthorchiasis in the BNF-exposed hamsters.
authors.forename: Latiporn
authors.initials: L
authors.lastname: Udomsuk
authors.forename: Waranya
authors.initials: W
authors.lastname: Chatuphonprasert
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
mesh_terms: D000208:Acute Disease; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology*; D020536:Enzyme Activators / Q000737:chemistry; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects*; D008647:Mesocricetus; D009889:Opisthorchiasis / Q000201:enzymology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D009891:Opisthorchis / Q000502:physiology; D012376:Rodent Diseases / Q000201:enzymology / Q000378:metabolism* / Q000469:parasitology; D019324:beta-Naphthoflavone / Q000737:chemistry
chemical_list: D000871:Anthelmintics; D004224:Diterpenes; D020536:Enzyme Activators; C030419:andrographolide; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2