title: Serological and Entomological Study of Dengue in Dang and Chitwan Districts of Nepal.
abstract: A descriptive cross-sectional study was carried out among 264 suspected dengue patients in two districts (Dang and Chitwan) of Nepal from June 2013 to November 2013. The anti-dengue IgM positivity was found to be (51/264)19.31% by capture ELISA, of which 21 (41.2%) were male and 30 (58.8%) were female. Symptoms of seropositive cases were fever, anorexia, nausea, headache, retro-orbital pain, skin rashes, and myalgia. Hematological features like thrombocytopenia and leucopenia were found to be significantly associated with the dengue fever (DF). Discarded tires were found as the commonest breeding habitats for the dengue vectors. Higher sero-positivity was recorded from the area having higher Breteau index (BI). The pH, chloride ion concentration and the salinity of the water from breeding habitats were found to be ranging from 6.9±0.82 to 8, 103.33±17.52 mg/L to 140.65 mg/L, and 0.19±0.032 ppt to 0.25 ppt respectively. This study may be helpful for the health authorities and public health workers for early diagnosis of DF and for the improved preventive measures to be adopted in the epidemic and possible epidemic areas.
authors.affiliation: Department of Microbiology, Trichandra multiple campus, Kathmandu, Nepal.
authors.forename: Rojina
authors.initials: R
authors.lastname: Shrestha
authors.affiliation: Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal.
authors.forename: Narayan Dutt
authors.initials: ND
authors.lastname: Pant
authors.affiliation: Department of Microbiology, Trichandra multiple campus, Kathmandu, Nepal.
authors.forename: Ganga
authors.initials: G
authors.lastname: Gc
authors.affiliation: Department of Microbiology, Trichandra multiple campus, Kathmandu, Nepal.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Thapa
authors.affiliation: Everest International Clinic and Research Center, Kathmandu, Nepal.
authors.forename: Biswas
authors.initials: B
authors.lastname: Neupane
authors.affiliation: Everest International Clinic and Research Center, Kathmandu, Nepal.
authors.forename: Yogendra
authors.initials: Y
authors.lastname: Shah
authors.affiliation: Institute of Science and Technology, Tribhuvan University, Natural History Museum, Kathmandu, Nepal.
authors.forename: Ishan
authors.initials: I
authors.lastname: Gautam
authors.affiliation: Everest International Clinic and Research Center, Kathmandu, Nepal.
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Pandey
mesh_terms: D000293:Adolescent; D000328:Adult; D000330:Aedes / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D001947:Breeding; D003715:Dengue / Q000097:blood* / Q000453:epidemiology*; D017753:Ecosystem; D006761:Hospitals; D006801:Humans; D007303:Insect Vectors / Q000254:growth & development; D007814:Larva / Q000254:growth & development; D008875:Middle Aged; D009390:Nepal / Q000453:epidemiology; D011795:Surveys and Questionnaires; D014867:Water; D055815:Young Adult
chemical_list: D014867:Water
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