title: An Amylase-Responsive Bolaform Supra-Amphiphile.
abstract: An amylase-responsive bolaform supra-amphiphile was constructed by the complexation between β-cyclodextrin and a bolaform covalent amphiphile on the basis of host-guest interaction. The bolaform covalent amphiphile could self-assemble in solution, forming sheet-like aggregates and displaying weak fluorescence because of aggregation-induced quenching. The addition of β-cyclodextrin led to the formation of the bolaform supra-amphiphile, prohibiting the aggregation of the bolaform covalent amphiphile and accompanying with the significant recovery of fluorescence. Upon the addition of α-amylase, with the degradation β-cyclodextrin, the fluorescence of the supra-amphiphile would quench gradually and significantly, and the quenching rate linearly correlated to the concentration of α-amylase. This study enriches the field of supra-amphiphiles on the basis of noncovalent interactions, and moreover, it may provide a facile way to estimate the activity of α-amylase.
authors.affiliation: Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Yuetong
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Zhengguo
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microsystems and Micronanostructures Manufacturing (Harbin Institute of Technology), Ministry of Education, Harbin 150080, P. R. China.
authors.forename: Guangtong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000681:Amylases / Q000737:chemistry* / Q000302:isolation & purification; D015374:Biosensing Techniques; D005453:Fluorescence; D012996:Solutions / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D012996:Solutions; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; D000681:Amylases; C031215:betadex
keywords: amylase; cyclodextrin; enzyme-responsive; self-assembly; supra-amphiphile