title: Surmounting a PCR challenge using a Contradictory matrix from the Theory of Inventive Problem Solving (TRIZ).
abstract: In this paper I tested whether Contradictory Matrix with 40 Inventive Principles, the simplest instrument from the Theory of Inventive Problem Solving (TRIZ), is a useful approach to a real-life PCR scenario. The PCR challenge consisted of standardization of fluorescence melting curve measurements in Competitive Amplification of Differentially Melting Amplicons (CADMA) PCR for multiple targets. Here I describe my way of using the TRIZ Matrix to generate seven alternative solutions from which I can choose the successful solution, consisting of repeated cycles of amplification and melting in a single PCR run.
authors.affiliation: Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacký University, Teaching Hospital Olomouc, Hněvotínská 5, 779 00 Olomouc, The Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Drábek
keywords: CADMA PCR; Contradictory matrix; Invention; TRIZ
references.citation: Vet Parasitol. 2010 Feb 26;168(1-2):11-8
references.pmid: 19931290
references.citation: J Mol Diagn. 2014 Mar;16(2):163-73
references.pmid: 24370857
references.citation: Expert Rev Gastroenterol Hepatol. 2015 Mar;9(3):273-6
references.pmid: 25579664
references.citation: Bioconjug Chem. 1994 Jan-Feb;5(1):3-7
references.pmid: 8199231
references.citation: Chem Biol. 2001 Jan;8(1):1-7
references.pmid: 11182314
references.citation: Hum Mutat. 2012 Jan;33(1):264-71
references.pmid: 21901793
references.citation: Nucleic Acids Res. 2000 Oct 1;28(19):3752-61
references.pmid: 11000267
references.citation: J Assist Reprod Genet. 2014 Nov;31(11):1427-36
references.pmid: 25241129
references.citation: BMC Cancer. 2012 Nov 23;12 :548
references.pmid: 23173730