title: Innovative High Gas Pressure Microscopy Chamber Designed for Biological Cell Observation.
abstract: An original high-pressure microscopy chamber has been designed for real-time visualization of biological cell growth during high isostatic (gas or liquid) pressure treatments up to 200 MPa. This new system is highly flexible allowing cell visualization under a wide range of pressure levels as the thickness and the material of the observation window can be easily adapted. Moreover, the design of the observation area allows different microscope objectives to be used as close as possible to the observation window. This chamber can also be temperature controlled. In this study, the resistance and optical properties of this new high-pressure chamber have been tested and characterized. The use of this new chamber was illustrated by a real-time study of the growth of two different yeast strains - Saccharomyces cerevisiae and Candida viswanathii - under high isostatic gas pressure (30 or 20 MPa, respectively). Using image analysis software, we determined the evolution of the area of colonies as a function of time, and thus calculated colony expansion rates.
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Ragon
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Hue
authors.initials: H
authors.lastname: Nguyen Thi Minh
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Guyot
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Loison
authors.affiliation: 2Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (EA3882),IFR 148,Université Européenne de Bretagne/Université de Brest/ESMISAB,Technopole Brest-Iroise,29280 Plouzané,France.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Burgaud
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Dupont
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Beney
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Gervais
authors.affiliation: 1UMR A 02.102 Procédés Alimentaires et Microbiologiques,Université Bourgogne Franche-Comté/AgroSup Dijon,1 Esplanade Erasme,21000 Dijon,France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Perrier-Cornet
mesh_terms: D002175:Candida / Q000166:cytology / Q000254:growth & development; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods*; D005740:Gases; D006874:Hydrostatic Pressure; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development
chemical_list: D005740:Gases
keywords: colony expansion rate; high-pressure microscopy; isostatic gas pressure; live-cell observation; optical chamber; yeast