title: Obesity-associated Inflammation Induces microRNA-155 Expression in Adipocytes and Adipose Tissue: Outcome on Adipocyte Function.
abstract: Obesity alters adipose tissue's metabolic and endocrine functions and causes a chronic local and systemic low-grade inflammatory state to develop, generating obesity-associated complications. In the last decade, many entities contributing to and regulating this inflammatory state have been identified, among which are microRNAs.
  
  
  
  This study aimed to identify microRNA regulated in inflamed adipocytes and adipose tissue, and its effect on adipocyte biology.
  
  
  
  We screened the expression profile of TNFα-treated adipocytes (a major pro-inflammatory protein expressed in obese adipose tissue), and identified miR-155 as the most responsive microRNA. The involvement of TNFα on the basal miR-155 expression was confirmed in the adipose tissue of Tnfa−/− mice where miR-155 was significantly reduced. Also, mice overexpressing p65 or invalidated for p65 in adipose tissue respectively increased and decreased miR-155 expression, in line with the involvement of the nuclear factor κB (NF-κB) pathway in miR-155 induction. miR-155 expression was higher in obese subjects' adipose tissue than in that of normal-weight subjects, and correlated with TNFα expression and body mass index. Gain and loss of function of miR-155 showed its effect on adipocyte function, probably via its ability to target PPARγ mRNA 3′UTR. Interestingly, miR-155 overexpression also resulted in an increased inflammatory state in adipocytes.
  
  
  
  Altogether, these data are evidence of a proinflammatory loop mediated by NF-κB and miR-155 that could participate in the amplification of inflammatory status in adipocytes.
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Esma
authors.initials: E
authors.lastname: Karkeni
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Astier
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Tourniaire
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Mouna
authors.initials: M
authors.lastname: El Abed
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Romier
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Gouranton
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Lin
authors.initials: L
authors.lastname: Wan
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Borel
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Salles
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Walrand
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Jianping
authors.initials: J
authors.lastname: Ye
authors.affiliation: Institut National de Recherche Agronomique (INRA) (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), UMR 1260, F-13385, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1062 (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.F.L.), Nutrition, Obésité et Risque Thrombotique F-13385, Marseille, France; Faculté de Médecine (E.K., J.A., F.T., M.E.A., B.R., E.G., P.B., J.-F.L.), Aix-Marseille Université, F-13385, Marseille, France; Pennington Biomedical Research Center (L.W., J.Y.), Louisiana State University System, Baton Rouge, LA 70808; Laboratory of Transplant Immunology, Regeneration Medicine Research Center, Sichuan University, Sichuan Province, 610041 China; and UMR INRA 1019 Unité de Nutrition Humaine (J.S., S.W.), Centre de Recherches INRA de Clermont-Ferrand/Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Landrier
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology*; D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007249:Inflammation / Q000209:etiology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D009765:Obesity / Q000150:complications*; D015398:Signal Transduction
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs
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