title: Mutational Analysis of Quinolone Resistance Protein QnrVC7 Provides Novel Insights into the Structure-Activity Relationship of Qnr Proteins.
abstract: This study assessed the functional importance of residues located at the i(-2) position of face 4 of the tandem repeat loops of the quinolone resistance protein QnrVC7 through mutagenesis studies. The i(-2) position of face 4 on different coils required residues with different natures. Some substitutions reduced the protective activity of QnrVC7, while some of them increased it. These findings advanced our understanding on the detailed structural organization and functional requirements of Qnr proteins.
authors.affiliation: Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, People's Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, People's Republic of China.
authors.forename: Kathy Hiu Laam
authors.initials: KH
authors.lastname: Po
authors.affiliation: Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, People's Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, People's Republic of China.
authors.forename: Edward Wai Chi
authors.initials: EW
authors.lastname: Chan
authors.affiliation: Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, People's Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, People's Republic of China sheng.chen@polyu.edu.hk.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000378:metabolism / Q000494:pharmacology*; D027081:DNA Gyrase / Q000378:metabolism; D004252:DNA Mutational Analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D014734:Vibrio cholerae / Q000187:drug effects / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; D002939:Ciprofloxacin; D027081:DNA Gyrase
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