title: Emerging From the Unknown: Structural and Functional Features of Agnoprotein of Polyomaviruses.
abstract: Agnoprotein is an important regulatory protein of polyomaviruses, including JCV, BKV, and SV40. In the absence of its expression, these viruses are unable to sustain their productive life cycle. It is a highly basic phosphoprotein that localizes mostly to the perinuclear area of infected cells, although a small amount of the protein is also found in nucleus. Much has been learned about the structure and function of this important regulatory protein in recent years. It forms highly stable dimers/oligomers in vitro and in vivo through its Leu/Ile/Phe-rich domain. Structural NMR studies revealed that this domain adopts an alpha-helix conformation and plays a critical role in the stability of the protein. It associates with cellular proteins, including YB-1, p53, Ku70, FEZ1, HP1α, PP2A, AP-3, PCNA, and α-SNAP; and viral proteins, including small t antigen, large T antigen, HIV-1 Tat, and JCV VP1; and significantly contributes the viral transcription and replication. This review summarizes the recent advances in the structural and functional properties of this important regulatory protein. J. Cell. Physiol. 231: 2115-2127, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Neuroscience, Laboratory of Molecular Neurovirology, MERB-757, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania.
authors.forename: A Sami
authors.initials: AS
authors.lastname: Saribas
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Laboratoire de Cristallographie et RMN Biologiques, 4 av. de l'Observatoire, Paris, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Coric
authors.affiliation: Department of Neuroscience, Laboratory of Molecular Neurovirology, MERB-757, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania.
authors.forename: Anahit
authors.initials: A
authors.lastname: Hamazaspyan
authors.affiliation: Department of Neuroscience, Laboratory of Molecular Neurovirology, MERB-757, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania.
authors.forename: William
authors.initials: W
authors.lastname: Davis
authors.affiliation: Department of Neuroscience, Laboratory of Molecular Neurovirology, MERB-757, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania.
authors.forename: Rachel
authors.initials: R
authors.lastname: Axman
authors.affiliation: Department of Neuroscience, Laboratory of Molecular Neurovirology, MERB-757, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania.
authors.forename: Martyn K
authors.initials: MK
authors.lastname: White
authors.affiliation: Moulder Center for Drug Discovery Research, Temple University School of Pharmacy, Philadelphia, Pennsylvania.
authors.forename: Magid
authors.initials: M
authors.lastname: Abou-Gharbia
authors.affiliation: Moulder Center for Drug Discovery Research, Temple University School of Pharmacy, Philadelphia, Pennsylvania.
authors.forename: Wayne
authors.initials: W
authors.lastname: Childers
authors.affiliation: Moulder Center for Drug Discovery Research, Temple University School of Pharmacy, Philadelphia, Pennsylvania.
authors.forename: Jon H
authors.initials: JH
authors.lastname: Condra
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Laboratoire de Cristallographie et RMN Biologiques, 4 av. de l'Observatoire, Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Bouaziz
authors.affiliation: Department of Neuroscience, Laboratory of Molecular Neurovirology, MERB-757, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Safak
mesh_terms: D000818:Animals; D000090266:Chromobox Protein Homolog 5; D006801:Humans; D007577:JC Virus / Q000302:isolation & purification / Q000378:metabolism; D011120:Polyomavirus / Q000302:isolation & purification / Q000378:metabolism*; D027601:Polyomavirus Infections / Q000821:virology*; D014157:Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism*
chemical_list: C000715594:CBX5 protein, human; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D000090266:Chromobox Protein Homolog 5
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