title: WSV399, a viral tegument protein, interacts with the shrimp protein PmVRP15 to facilitate viral trafficking and assembly.
abstract: Viral responsive protein 15 (PmVRP15) has been identified as a highly up-regulated gene in the hemocyte of white spot syndrome virus (WSSV)-infected shrimp Penaeus monodon. However, the function of PmVRP15 in host-viral interaction was still unclear. To elucidate PmVRP15 function, the interacting partner of PmVRP15 from WSSV was screened by yeast two-hybrid assay and then confirmed by co-immunoprecipitation (Co-IP). Only WSV399 protein was identified as a PmVRP15 binding protein; however, the function of WSV399 has not been characterized. Localization of WSV399 on the WSSV virion was revealed by immunoblotting analysis (in vitro) and immunoelectron microscopy (in vivo). The results showed that WSV399 is a structural protein of the WSSV virion and is particularly located on the tegument. Gene silencing of wsv399 in WSSV-infected shrimp reduced the percentage of cumulative mortality by 74%, although the expression level of a viral replication marker gene, vp28, was not changed suggesting that WSV399 might not involved in viral replication but viral assembly. Because it has already been known that tegument proteins function in capsid transport during viral trafficking and assembly, interaction between PmVRP15 on hemocyte nuclear membrane and the WSV399 viral tegument protein suggests that PmVRP15 might be required for trafficking and assembly of WSSV during infection.
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Bangkok 10300, Thailand.
authors.forename: Phattarunda
authors.initials: P
authors.lastname: Jaree
authors.affiliation: Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.affiliation: Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Lo
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Bangkok 10300, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Bangkok 10300, Thailand. Electronic address: kunlaya.s@chula.ac.th.
authors.forename: Kunlaya
authors.initials: K
authors.lastname: Somboonwiwat
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism; D006434:Hemocytes / Q000276:immunology / Q000821:virology; D054884:Host-Pathogen Interactions / Q000502:physiology*; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D021381:Protein Transport; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*; D019065:Virus Assembly / Q000235:genetics*; D053586:Virus Internalization; D046848:White spot syndrome virus 1 / Q000235:genetics*
chemical_list: C548473:VP28 protein, white spot syndrome virus; D014759:Viral Envelope Proteins
keywords: Host-viral interaction; Penaeus monodon; PmVRP15; WSV399; White spot syndrome virus