title: Sinomenine attenuates airway inflammation and remodeling in a mouse model of asthma.
abstract: Asthma is an inflammatory disease that involves airway inflammation and remodeling. Sinomenine (SIN) has been demonstrated to have immunosuppressive and anti‑inflammatory properties. The aim of the present study was to investigate the inhibitory effects of SIN on airway inflammation and remodeling in an asthma mouse model and observe the effects of SIN on the transforming growth factor‑β1 (TGF‑β1)/connective tissue growth factor (CTGF) pathway and oxidative stress. Female BALB/c mice were sensitized by repetitive ovalbumin (OVA) challenge for 6 weeks in order to develop a mouse model of asthma. OVA‑sensitized animals received SIN (25, 50 and 75 mg/kg) or dexamethasone (2 mg/kg). A blank control group received saline only. The area of smooth muscle and collagen, levels of mucus secretion and inflammatory cell infiltration were evaluated 24 h subsequent to the final OVA challenge. mRNA and protein levels of TGF‑β1 and CTGF were determined by reverse transcription‑quantitative polymerase chain reaction and immunohistology, respectively. The indicators of oxidative stress were detected by spectrophotometry. SIN significantly reduced allergen‑induced increases in smooth muscle thickness, mucous gland hypertrophy, goblet cell hyperplasia, collagen deposition and eosinophilic inflammation. The levels of TGF‑β1 and CTGF mRNA and protein were significantly reduced in the lungs of mice treated with SIN. Additionally, the total antioxidant capacity was increased in lungs following treatment with SIN. The malondialdehyde content and myeloperoxidase activities in the lungs from OVA‑sensitized mice were significantly inhibited by SIN. In conclusion, SIN may inhibit airway inflammation and remodeling in asthma mouse models, and may have therapeutic efficacy in the treatment of asthma.
authors.affiliation: Department of Gerontal Respiratory Medicine, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Bao
authors.affiliation: Department of Gerontal Respiratory Medicine, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
authors.forename: Xiao-Ju
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Department of Rheumatoid Bone, Chinese Medicine Hospital of Lanzhou City, Lanzhou, Gansu 730050, P.R. China.
authors.forename: Yun-Lin
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Gerontal Respiratory Medicine, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Men
authors.affiliation: Department of Gerontal Respiratory Medicine, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zeng
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy*; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D005260:Female; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008807:Mice, Inbred BALB C; D009019:Morphinans / Q000494:pharmacology*; D018384:Oxidative Stress
chemical_list: D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; C526318:CCN2 protein, mouse; D009019:Morphinans; D055513:Connective Tissue Growth Factor; C009271:sinomenine; D003907:Dexamethasone
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