title: In vivo burn diagnosis by camera-phone diffuse reflectance laser speckle detection.
abstract: Burn diagnosis using laser speckle light typically employs widefield illumination of the burn region to produce two-dimensional speckle patterns from light backscattered from the entire irradiated tissue volume. Analysis of speckle contrast in these time-integrated patterns can then provide information on burn severity. Here, by contrast, we use point illumination to generate diffuse reflectance laser speckle patterns of the burn. By examining spatiotemporal fluctuations in these time-integrated patterns along the radial direction from the incident point beam, we show the ability to distinguish partial-thickness burns in a porcine model in vivo within the first 24 hours post-burn. Furthermore, our findings suggest that time-integrated diffuse reflectance laser speckle can be useful for monitoring burn healing over time post-burn. Unlike conventional diffuse reflectance laser speckle detection systems that utilize scientific or industrial-grade cameras, our system is designed with a camera-phone, demonstrating the potential for burn diagnosis with a simple imager.
authors.affiliation: Biomedical Engineering Department, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, POB 653, Be'er-Sheva 8410501, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Ragol
authors.affiliation: Biomedical Engineering Department, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, POB 653, Be'er-Sheva 8410501, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Remer
authors.affiliation: Department of Plastic and Reconstructive Surgery, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Rager Blvd, POB 151, Be'er-Sheva 8410101, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Shoham
authors.affiliation: Biomedical Engineering Department, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, POB 653, Be'er-Sheva 8410501, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Hazan
authors.affiliation: Lahav CRO Research Unit, POB Negev, Kibbutz Lahav, 8533500, Israel.
authors.forename: U
authors.initials: U
authors.lastname: Willenz
authors.affiliation: Institute of Pathology, Soroka University Medical Center, POB 151, Be'er-Sheva 8410101, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Sinelnikov
authors.affiliation: Institute of Pathology, Soroka University Medical Center, POB 151, Be'er-Sheva 8410101, Israel.
authors.forename: V
authors.initials: V
authors.lastname: Dronov
authors.affiliation: Department of Plastic and Reconstructive Surgery, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Rager Blvd, POB 151, Be'er-Sheva 8410101, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Rosenberg
authors.affiliation: Biomedical Engineering Department, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, POB 653, Be'er-Sheva 8410501, Israel; Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, POB 653, Be'er-Sheva 8410501, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Bilenca
keywords: (030.6140) Speckle; (170.3890) Medical optics instrumentation; (170.4580) Optical diagnostics for medicine
references.citation: Phys Med Biol. 2013 Jun 7;58(11):R37-61
references.pmid: 23666068
references.citation: Biomed Opt Express. 2013 Sep 26;4(10):2269-83
references.pmid: 24156082
references.citation: Opt Lett. 2012 Oct 1;37(19):3957-9
references.pmid: 23027244
references.citation: Opt Lett. 2013 May 1;38(9):1401-3
references.pmid: 23632498
references.citation: Burns. 2008 Sep;34(6):761-9
references.pmid: 18511202
references.citation: Burns. 1993 Dec;19(6):485-9
references.pmid: 8292231
references.citation: Burns. 2011 May;37(3):377-86
references.pmid: 21185123
references.citation: IEEE Trans Biomed Eng. 2010 May;57(5):1152-7
references.pmid: 20142159
references.citation: Appl Opt. 2015 Aug 10;54(23 ):7099-105
references.pmid: 26368382
references.citation: J Biomed Opt. 2006 Mar-Apr;11(2):021006
references.pmid: 16674181
references.citation: Appl Opt. 1995 May 1;34(13):2362-6
references.pmid: 21037790
references.citation: Burns. 2005 Sep;31(6):744-52
references.pmid: 16129229
references.citation: J Biophotonics. 2015 Nov;8(11-12):902-7
references.pmid: 25908015
references.citation: J Biomed Opt. 2015 Aug;20(8):86009
references.pmid: 26271055
references.citation: Appl Opt. 1996 Oct 1;35(28):5727-35
references.pmid: 21127582
references.citation: Opt Lett. 2002 Apr 1;27(7):533-5
references.pmid: 18007856
references.citation: J Biomed Opt. 2010 Jan-Feb;15(1):011109
references.pmid: 20210435
references.citation: Burns. 2004 Nov;30(7):684-90
references.pmid: 15475143
references.citation: Burns. 2001 May;27(3):233-9
references.pmid: 11311516
references.citation: Burns. 2009 Feb;35(1):36-45
references.pmid: 18952377
references.citation: Biomed Opt Express. 2014 Sep 08;5(10):3467-81
references.pmid: 25360365