title: Reutilization of green liquor chemicals for pretreatment of whole rice waste biomass and its application to 2,3-butanediol production.
abstract: The performance of green liquor pretreatment using Na2CO3 and Na2SO3 and its optimization for whole rice waste biomass (RWB) was investigated. Incubation of Na2CO3-Na2SO3 at a 1:1 ratio (chemical charge 10%) for 12% RWB at 100°C for 6h resulted in maximum delignification (58.2%) with significant glucan yield (88%) and total sugar recovery (545mg/g of RWB) after enzymatic hydrolysis. Recovery and reusability of the resulting chemical spent wash were evaluated to treat RWB along with its compatibility for enzymatic digestibility. Significant hydrolysis and lignin removal were observed for up to three cycles; however, further reuse of Na2CO3 and Na2SO3 lowered their performance. Significant 2,3-butanediol (BDO) was produced by Klebsiella pneumoniae KMK-05 with the RWB enzymatic hydrolysate from each pretreatment cycle. BDO yield achieved using RWB-derived sugars was similar to those using laboratory-grade sugars. This pretreatment strategy constitutes an ecofriendly, cost-effective, and practical method for utilizing lignocellulosic biomass.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 136-713, South Korea.
authors.forename: Ganesh D
authors.initials: GD
authors.lastname: Saratale
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 136-713, South Korea.
authors.forename: Moo-Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 136-713, South Korea. Electronic address: mkoh@korea.ac.kr.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000378:metabolism*; D002241:Carbohydrates / Q000096:biosynthesis / Q000737:chemistry; D002254:Carbonates / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D006868:Hydrolysis; D007711:Klebsiella pneumoniae / Q000378:metabolism; D008031:Lignin / Q000737:chemistry / Q000302:isolation & purification; D012275:Oryza / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry; D014866:Waste Products
chemical_list: D002072:Butylene Glycols; D002241:Carbohydrates; D002254:Carbonates; D005936:Glucans; D013431:Sulfates; D014866:Waste Products; C012036:sodium sulfate; C026978:2,3-butylene glycol; C005686:sodium carbonate; D008031:Lignin
keywords: 2,3-Butanediol; Na(2)CO(3)–Na(2)SO(3) pretreatment; Reusability of chemicals; Rice waste biomass