title: Determining the order of resistance genes against Stagonospora nodorum blotch, Fusarium head blight and stem rust on wheat chromosome arm 3BS.
abstract: BACKGROUND
  Stagonospora nodorum blotch (SNB), Fusarium head blight (FHB) and stem rust (SR), caused by the fungi Parastagonospora (synonym Stagonospora) nodorum, Fusarium graminearum and Puccinia graminis, respectively, significantly reduce yield and quality of wheat. Three resistance factors, QSng.sfr-3BS, Fhb1 and Sr2, conferring resistance, respectively, to SNB, FHB and SR, each from a unique donor line, were mapped previously to the short arm of wheat chromosome 3B. Based on published reports, our hypothesis was that Sr2 is the most distal, Fhb1 the most proximal and QSng.sfr-3BS is in between Sr2 and Fhb1 on wheat chromosome arm 3BS.
  
  
  RESULTS
  To test this hypothesis, 1600 F2 plants from crosses between parental lines Arina, Alsen and Ocoroni86, conferring resistance genes QSng.sfr-3BS, Fhb1 and Sr2, respectively, were genotyped and phenotyped for SNB along with the parental lines. Five closely linked single-nucleotide polymorphism (SNP) markers were used to make the genetic map and determine the gene order.
  
  
  CONCLUSIONS
  The results indicate that QSng.sfr-3BS is located between the other two resistance genes on chromosome 3BS. Knowing the positional order of these resistance genes will aid in developing a wheat line with all three genes in coupling, which has the potential to provide broad-spectrum resistance preventing grain yield and quality losses.
authors.affiliation: Department of Agronomy, Purdue University, 915 W. State Street, West Lafayette, IN, 47907-2054, USA. rthapa@purdue.edu.
authors.forename: Rima
authors.initials: R
authors.lastname: Thapa
authors.affiliation: USDA-ARS Plant Science Research, North Carolina State University, Raleigh, NC, 27695-7620, USA. Gina.Brown-Guedira@ars.usda.gov.
authors.forename: Gina
authors.initials: G
authors.lastname: Brown-Guedira
authors.affiliation: Department of Agronomy, Purdue University, 915 W. State Street, West Lafayette, IN, 47907-2054, USA. hohm@purdue.edu.
authors.forename: Herbert W
authors.initials: HW
authors.lastname: Ohm
authors.affiliation: Monsanto Company, 1982 State Route 46, Stonington, IL, 62567, USA. maria.mateos.hernandez@monsanto.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Mateos-Hernandez
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, 915 W. State Street, West Lafayette, IN, 47907-2054, USA. kawise@purdue.edu.
authors.forename: Kiersten A
authors.initials: KA
authors.lastname: Wise
authors.affiliation: USDA-ARS, Department of Botany and Plant Pathology, Purdue University, 915 West State Street, West Lafayette, IN, 47907-2054, USA. Steve.Goodwin@ARS.USDA.gov.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Goodwin
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001487:Basidiomycota / Q000502:physiology*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics*; D005670:Fusarium / Q000502:physiology*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018547:Plant Stems / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D005819:Genetic Markers
references.citation: Genome. 2003 Aug;46(4):546-54
references.pmid: 12897862
references.citation: Theor Appl Genet. 2006 Feb;112(3):492-9
references.pmid: 16311724
references.citation: Annu Rev Phytopathol. 2011;49:465-81
references.pmid: 21568701
references.citation: Nucleic Acids Res. 1991 Jul 25;19(14):4008
references.pmid: 1861999
references.citation: Theor Appl Genet. 2005 Jul;111(2):325-36
references.pmid: 15895203
references.citation: Genetics. 2008 Jan;178(1):489-511
references.pmid: 18202390
references.citation: Genetics. 1989 Jan;121(1):185-99
references.pmid: 2563713
references.citation: IEEE/ACM Trans Comput Biol Bioinform. 2011 Mar-Apr;8(2):381-94
references.pmid: 20479505
references.citation: Phytopathology. 2011 Dec;101(12):1418-23
references.pmid: 22070278
references.citation: Theor Appl Genet. 2011 Mar;122(4):735-44
references.pmid: 21060985
references.citation: Cell Microbiol. 2008 Jul;10(7):1421-8
references.pmid: 18384660
references.citation: Methods Mol Biol. 2012;835:191-207
references.pmid: 22183655
references.citation: Theor Appl Genet. 2014 Mar;127(3):573-86
references.pmid: 24306318
references.citation: Theor Appl Genet. 2003 Nov;107(7):1226-34
references.pmid: 12928778
references.citation: Nat Protoc. 2008;3(9):1452-6
references.pmid: 18772872
references.citation: Plant Dis. 2000 Feb;84(2):203
references.pmid: 30841334
references.citation: Theor Appl Genet. 2009 Aug;119(3):531-9
references.pmid: 19471905
references.citation: Theor Appl Genet. 2007 Sep;115(5):697-708
references.pmid: 17634916
references.citation: Mol Plant Microbe Interact. 2008 Mar;21(3):287-93
references.pmid: 18257678
references.citation: Theor Appl Genet. 2003 Nov;107(7):1235-42
references.pmid: 12898031
references.citation: Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13544-9
references.pmid: 20624958
references.citation: Annu Rev Phytopathol. 2004;42:135-61
references.pmid: 15283663
references.citation: Plant Biotechnol J. 2013 Jan;11(1):23-32
references.pmid: 23046423
references.citation: Phytopathology. 2011 Nov;101(11):1278-84
references.pmid: 21770777