title: Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus.
abstract: Heat shock proteins play essential roles in basic cellular events. Spawning migration is a complex process, with significant structural and biochemical changes taking place in the adult gonad. To date, the molecular mechanisms underlying migration reproductive biology remain undetermined. In this regard, a full length HSP90AA1 comprising 2608 nucleotides from the anadromous fish Coilia nasus was characterized, encoding 742 amino acid (aa) residues with potential phosphorylation sites. HSP90AA1 mRNA transcripts were detected in all organs, especially in the gonad. Furthermore, the greatest transcript levels were found during the developmental phase, while the lowest levels were found during the resting phase. In addition, the strongest immunolabeling positive signal was found in the primary spermatocyte and oocyte, with lower positive staining in secondary germ cells, and a weak or absent level in the mature sperm and oocyte. Interestingly, HSP90AA1 was mainly located in the cytoplasm of germ cells. These results are important for understanding the molecular mechanism of anadromous migration reproductive biology. In combination with data from other fish species, the result of this present study may facilitate further investigations on the spawning migration mechanism.
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. fangda@ffrc.cn.|Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Xuejiali 69, Wuxi 214128, China. fangda@ffrc.cn.
authors.forename: Di-An
authors.initials: DA
authors.lastname: Fang
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. duanjr@ffrc.cn.
authors.forename: Jin-Rong
authors.initials: JR
authors.lastname: Duan
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. zhouyf@ffrc.cn.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Zhou
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. zhangmy@ffrc.cn.
authors.forename: Min-Ying
authors.initials: MY
authors.lastname: Zhang
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. xudp@ffrc.cn.|Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Xuejiali 69, Wuxi 214128, China. xudp@ffrc.cn.
authors.forename: Dong-Po
authors.initials: DP
authors.lastname: Xu
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. liuk@ffrc.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China. xup@ffrc.cn.
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
keywords: Coilia nasus; HSP90AA1 expression; anadromous fish; migration mechanism
references.citation: Biochim Biophys Acta. 2014 Feb;1839(2):71-87
references.pmid: 24389346
references.citation: Gen Comp Endocrinol. 2010 Feb 1;165(3):367-89
references.pmid: 19505465
references.citation: Prog Neurobiol. 2010 Oct;92(2):184-211
references.pmid: 20685377
references.citation: Annu Rev Biochem. 2006;75:271-94
references.pmid: 16756493
references.citation: Mar Genomics. 2015 Dec;24 Pt 3:241-3
references.pmid: 26112895
references.citation: J Signal Transduct. 2012;2012:181560
references.pmid: 23209895
references.citation: Biochim Biophys Acta. 2011 Dec;1816(2):89-104
references.pmid: 21605630
references.citation: Pharmacol Ther. 1998 Aug;79(2):129-68
references.pmid: 9749880
references.citation: Fish Shellfish Immunol. 2014 Dec;41(2):428-36
references.pmid: 25270529
references.citation: Pharmaceuticals (Basel). 2013 Nov 11;6(11):1407-28
references.pmid: 24287464
references.citation: Cryobiology. 2015 Apr;70(2):164-9
references.pmid: 25578982
references.citation: Methods. 2007 Nov;43(3):229-37
references.pmid: 17920520
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2011 Jun;159(2):150-8
references.pmid: 21349342
references.citation: Trends Biochem Sci. 1999 Apr;24(4):136-41
references.pmid: 10322418
references.citation: Biochimie. 2008 Apr;90(4):555-62
references.pmid: 17825976
references.citation: Rev Physiol Biochem Pharmacol. 2004;151:1-44
references.pmid: 14740253
references.citation: Semin Cell Dev Biol. 2015 Apr;40:142-52
references.pmid: 25770416
references.citation: Proc Natl Acad Sci U S A. 2007 May 15;104(20):8346-51
references.pmid: 17483452
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2004 Nov;139(3):383-400
references.pmid: 15544963
references.citation: Gen Comp Endocrinol. 2010 Feb 1;165(3):390-411
references.pmid: 19348807
references.citation: Mar Genomics. 2015 Jun;21:17-9
references.pmid: 25733195
references.citation: Reproduction. 2009 Jul;138(1):65-80
references.pmid: 19423662
references.citation: Angew Chem Int Ed Engl. 2002 Apr 2;41(7):1098-113
references.pmid: 12491239
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Cell Rep. 2013 Jun 27;3(6):2179-90
references.pmid: 23791531