title: Using Zebrafish to Implement a Course-Based Undergraduate Research Experience to Study Teratogenesis in Two Biology Laboratory Courses.
abstract: A course-based undergraduate research experience (CURE) spanning three semesters was introduced into freshman and sophomore biology classes, with the hypothesis that participation in a CURE affects skills in research, communication, and collaboration, which may help students persist in science. Student research projects were centered on the hypothesis that nicotine and caffeine exposure during early development affects gastrulation and heart development in zebrafish. First, freshmen generated original data showing distinct effects of embryonic nicotine and caffeine exposure on zebrafish heart development and function. Next, Cell Biology laboratory students continued the CURE studies and identified novel teratogenic effects of nicotine and caffeine during gastrulation. Finally, new freshmen continued the CURE research, examining additional toxicant effects on development. Students designed new protocols, made measurements, presented results, and generated high-quality preliminary data that were studied in successive semesters. By implementing this project, the CURE extended faculty research and provided a scalable model to address national goals to involve more undergraduates in authentic scientific research. In addition, student survey results support the hypothesis that CUREs provide significant gains in student ability to (1) design experiments, (2) analyze data, and (3) make scientific presentations, translating into high student satisfaction and enhanced learning.
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana.
authors.forename: Swapnalee
authors.initials: S
authors.lastname: Sarmah
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana.
authors.forename: Grady W
authors.initials: GW
authors.lastname: Chism
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Vaughan
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana.
authors.forename: Pooja
authors.initials: P
authors.lastname: Muralidharan
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana.
authors.forename: Jim A
authors.initials: JA
authors.lastname: Marrs
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana.
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Marrs
mesh_terms: D000818:Animals; D001695:Biology / Q000193:education*; D012586:Science / Q000193:education*; D013334:Students; D064793:Teratogenesis; D014495:Universities; D015027:Zebrafish / Q000002:abnormalities*
references.citation: Eur J Pharmacol. 2008 Aug 20;590(1-3):264-8
references.pmid: 18647709
references.citation: Reprod Toxicol. 1993 Sep-Oct;7(5):449-52
references.pmid: 8274820
references.citation: FASEB J. 2009 Apr;23(4):1272-8
references.pmid: 19088180
references.citation: Adv Physiol Educ. 2008 Jun;32(2):136-41
references.pmid: 18539852
references.citation: Dev Dyn. 2016 Mar;245(3):244-58
references.pmid: 26434660
references.citation: Science. 2008 Oct 31;322(5902):684-5
references.pmid: 18974335
references.citation: CBE Life Sci Educ. 2010 Spring;9(1):55-69
references.pmid: 20194808
references.citation: Pediatr Pulmonol. 2014 Jan;49(1):67-75
references.pmid: 23401386
references.citation: CBE Life Sci Educ. 2014 Spring;13(1):29-40
references.pmid: 24591501
references.citation: J Gen Physiol. 1945 Nov 20;29:63-72
references.pmid: 21005099
references.citation: Neuropharmacology. 2015 Jan;88:187-98
references.pmid: 24950455
references.citation: Int J Obes (Lond). 2015 Apr;39(4):658-64
references.pmid: 25388405
references.citation: Zebrafish. 2014 Aug;11(4):384-95
references.pmid: 24941301
references.citation: Science. 2007 Dec 21;318(5858):1872-3
references.pmid: 18096791
references.citation: Paediatr Respir Rev. 2013 Mar;14(1):3-8
references.pmid: 23347655
references.citation: Annu Rev Cell Dev Biol. 2009;25:93-112
references.pmid: 19575638
references.citation: Alcohol. 2010 Nov-Dec;44(7-8):707-15
references.pmid: 20036484
references.citation: CBE Life Sci Educ. 2015 Summer;14(2):14:ar21
references.pmid: 26033869
references.citation: Zebrafish. 2009 Jun;6(2):145-60
references.pmid: 19537942
references.citation: J Pharmacol Exp Ther. 2008 Jan;324(1):331-41
references.pmid: 17947495
references.citation: CBE Life Sci Educ. 2011 Fall;10(3):279-86
references.pmid: 21885824
references.citation: Biol Open. 2013 Aug 14;2(10):1013-21
references.pmid: 24167711
references.citation: N Engl J Med. 2013 Aug 1;369(5):448-57
references.pmid: 23902484
references.citation: J Am Acad Child Adolesc Psychiatry. 2001 Jun;40(6):630-41
references.pmid: 11392340
references.citation: Am J Physiol Regul Integr Comp Physiol. 2014 Dec 15;307(12):R1471-87
references.pmid: 25354728
references.citation: Alcohol. 2015 Mar;49(2):149-63
references.pmid: 25541501
references.citation: Dev Dyn. 2013 Oct;242(10):1184-201
references.pmid: 23832875
references.citation: Cell Biol Educ. 2004 Winter;3(4):270-7
references.pmid: 15592600
references.citation: Pediatr Res. 2003 Feb;53(2):266-73
references.pmid: 12538785
references.citation: Am J Respir Crit Care Med. 2001 Sep 15;164(6):989-94
references.pmid: 11587984
references.citation: Science. 2007 Apr 27;316(5824):548-9
references.pmid: 17463273
references.citation: CBE Life Sci Educ. 2014 Winter;13(4):711-23
references.pmid: 25452493
references.citation: J Undergrad Neurosci Educ. 2011 Oct 15;10(1):A24-36
references.pmid: 23626490
references.citation: Toxicol Sci. 2010 Aug;116(2):364-74
references.pmid: 20363831
references.citation: Toxicol Appl Pharmacol. 2015 Apr 1;284(1):65-78
references.pmid: 25668718
references.citation: Science. 2013 Sep 27;341(6153):1455-6
references.pmid: 24072909
references.citation: Science. 2015 Feb 6;347(6222):1261757
references.pmid: 25657254
references.citation: CBE Life Sci Educ. 2015 Mar 2;14(1):es1
references.pmid: 25687826
references.citation: Toxicology. 2005 May 5;209(3):245-61
references.pmid: 15795061
references.citation: PLoS Biol. 2011 Nov;9(11):e1001174
references.pmid: 22110400