title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii.
abstract: Cotton is an economically important crop throughout the world, and is a pioneer crop in salt stress tolerance research. Investigation of the genetic regulation of salinity tolerance will provide information for salt stress-resistant breeding. Here, we employed next-generation RNA-Seq technology to elucidate the salt-tolerant mechanisms in cotton using the diploid cotton species Gossypium davidsonii which has superior stress tolerance. A total of 4744 and 5337 differentially expressed genes (DEGs) were found to be involved in salt stress tolerance in roots and leaves, respectively. Gene function annotation elucidated salt overly sensitive (SOS) and reactive oxygen species (ROS) signaling pathways. Furthermore, we found that photosynthesis pathways and metabolism play important roles in ion homeostasis and oxidation balance. Moreover, our studies revealed that alternative splicing also contributes to salt-stress responses at the posttranscriptional level, implying its functional role in response to salinity stress. This study not only provides a valuable resource for understanding the genetic control of salt stress in cotton, but also lays a substantial foundation for the genetic improvement of crop resistance to salt stress.
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Guozhong
authors.initials: G
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chaoze
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Yunnan Ice Harbor Biotechnology Co. Ltd, Kunming 650000, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Hybrid Cotton R &D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wangzhen
authors.initials: W
authors.lastname: Guo
mesh_terms: D017398:Alternative Splicing; D004171:Diploidy; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics / Q000254:growth & development*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D058977:Molecular Sequence Annotation; D018749:RNA, Plant / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D055049:Salt Tolerance; D017423:Sequence Analysis, RNA / Q000379:methods*; D059467:Transcriptome
chemical_list: D018749:RNA, Plant; D017382:Reactive Oxygen Species
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