title: Enhancement of sodium taurocholate to the absorption of cefquinome.
abstract: The objective of the research was to detect the enhancement effect of sodium taurocholate on the absorption of cefquinome both in Caco-2 cells and rats. The absorption efficiency of cefquinome was determined by high performance liquid chromatography and calculated with apparent permeability coefficients (Papp) after Caco-2 cell monolayers treated odium taurocholate(2 mmol/L) and cefquinome. The results showed that the absorption of cefquinome in Caco-2 cell monolayers was significantly increased with the sodium taurocholate (2mmol/L). Similar results were also detected in the rats orally administrated with 1 mL PBS of cefquinome (20mg/mL) containing different concentration of sodium taurocholate (5 mmol/L, 10mmol/L and 20mmol/L) respectively. Compared with control group, sodium taurocholate at 10 and 20 mmol/L increased the absorption of cefquinome in rats from 0.26±0.04μg/mL to 0.57±0.03μg/mL, 0.78 ±0.07μg/mL respectively. These results indicated that sodium taurocholate could increase the intestinal permeability in a concentration-dependent mode, which will be useful for clinical treatment.
authors.affiliation: College of Veterinary Medicines, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicines, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Cao
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicines, Nanjing Agricultural University, Nanjing, China.
authors.forename: Shan-xiang
authors.initials: SX
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicines, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D002511:Cephalosporins / Q000493:pharmacokinetics* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D051381:Rats; D013656:Taurocholic Acid / Q000494:pharmacology*
chemical_list: D002511:Cephalosporins; D013656:Taurocholic Acid; C068212:cefquinome