title: Quantitative assessments of glycolysis from single cells.
abstract: The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (18F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system forin vitro18F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Young Shik
authors.initials: YS
authors.lastname: Shin
authors.affiliation: NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Jungwoo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: Dazy
authors.initials: D
authors.lastname: Johnson
authors.affiliation: Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USA.
authors.forename: Alex A
authors.initials: AA
authors.lastname: Dooraghi
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: Wilson X
authors.initials: WX
authors.lastname: Mai
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Ta
authors.affiliation: Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USA.
authors.forename: Arion F
authors.initials: AF
authors.lastname: Chatziioannou
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USA; NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Phelps
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Nathanson
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Heath
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