title: Pharmaco-Phylogenetic Investigation of Methyl Gallate Isolated from Acacia nilotica (L.) Delile and Its Cytotoxic Effect on NIH3T3 Mouse Fibroblast.
abstract: Present exploration deals with the therapeutic perspective of methyl gallate isolated from the leaf extract of Acacia nilotica (L.) Delile in contrast to food-borne bacterial pathogen's viz., Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus aureus with their evolutionary succession. The extract was subjected to phytochemical analysis and isolated compound was identified as methyl gallate using UV-vis, IR and NMR spectra. It was found most potent against K. pneumoniae with its minimum inhibition concentration (MIC) of 0.32 mg/ml and minimum bactericidal concentration (MBC) at 0.62 mg/ml. The correlation of MIC values with an evolutionary succession assists the relationship between their genetic and toxic properties. The cytotoxic pursuit of methyl gallate was additionally assessed over NIH3T3 mouse fibroblast by Neutral red (NR) uptake, MTT cell proliferation assay and did not disclose any relevant influence on cell viability as well as cell proliferation. As such, the methyl gallate extracted from the leaf of A. nilotica holds massive antibacterial aptitude and hands out towards a new paradigm for food and pharmaceutical industries.
authors.forename: Rohit K
authors.initials: RK
authors.lastname: Mishra
authors.forename: M
authors.initials: M
authors.lastname: Ramakrishna
authors.forename: Vani
authors.initials: V
authors.lastname: Mishra
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Pathak
authors.forename: S
authors.initials: S
authors.lastname: Rajesh
authors.forename: Shivesh
authors.initials: S
authors.lastname: Sharma
authors.forename: Avinash C
authors.initials: AC
authors.lastname: Pandey
authors.forename: G
authors.initials: G
authors.lastname: Nageswara Rao
authors.affiliation: Biological Product Laboratory, Department of Botany, University of Allahabad, Allahabad-211002, India. anupambplau@rediffmail.com.
authors.forename: Anupam
authors.initials: A
authors.lastname: Dikshit
mesh_terms: D000045:Acacia; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002470:Cell Survival / Q000187:drug effects; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D010802:Phylogeny; D010936:Plant Extracts; D018515:Plant Leaves
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; C052082:methyl gallate; D005707:Gallic Acid