title: A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei.
abstract: Enterotoxigenic Escherichia coli (ETEC) infection is the major cause of diarrhea in neonatal piglets. The fimbriae as colonizing factor in the pathogenesis of ETEC constitute a primary target for vaccination against ETEC. Lactic acid bacteria (LAB) are attractive tools to deliver antigens at the mucosal level. With the safety of genetically modified LAB in mind, a food-grade secretion vector (pALRc or pALRb) was constructed with DNA entirely from LAB, including the replicon, promoter, signal peptide, and selection marker alanine racemase gene (alr). To evaluate the feasibility of the system, the nuclease gene (nuc) from Staphylococcus aureus was used as a reporter to be expressed in both Lactococcus lactis and Lactobacillus casei. Subsequently, the extracellular secretion of the fimbrial adhesin FaeG of ETEC was confirmed by Western blot analysis. These results showed that this food-grade expression system has potential as the delivery vehicle for the safe use of genetically modified LAB for the development of vaccines against ETEC infection.
authors.affiliation: a State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: W W
authors.initials: WW
authors.lastname: Lu
authors.affiliation: b Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology, Jinan 250103, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Xin
authors.affiliation: a State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Kong
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics; D022361:Escherichia coli Vaccines / Q000235:genetics; D005822:Genetic Vectors; D007780:Lacticaseibacillus casei / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics; D013552:Swine
chemical_list: D018830:Adhesins, Escherichia coli; D022361:Escherichia coli Vaccines; C494388:FaeG protein, E coli
keywords: Escherichia coli entérotoxinogène; bactéries lactiques; diarrhées néonatales; enterotoxigenic Escherichia coli; food-grade expression system; lactic acid bacteria; neonatal diarrhea; pigs; porcs; système d’expression de qualité alimentaire; vaccine delivery vehicle; véhicules d’administration de vaccins