title: [Application of eight-probe fluorescence in situ hybridization and R-banding karyotype analysis for the diagnosis of acute lymphoblastic leukemia].
abstract: OBJECTIVE
  To assess the value of eight-probe fluorescence in situ hybridization (FISH) and R-banding karyotype analysis for the diagnosis of acute lymphoblastic leukemia (ALL).
  
  
  METHODS
  With the eight-probe FISH (using probes for MYC, P16, E2A, CHIC2/D10Z1/D17Z1, TEL/AMLl, MLL, BCR/ABL1, and IGH) and R-banding karyotype analysis, 237 cases of ALL were analyzed.
  
  
  RESULTS
  Cytogenetic changes were detected in 135 (56.96%) of all cases, which have involved MYC, P16, E2A, CHIC2/D10Z1/D17Z1, TEL/AMLl, MLL, BCR/ABL1, and IGH polyploidies. R-banding karyotype analysis has only detected abnormalities in 48 of such cases, in addition with 14 abnormalities missed by the FISH probes, which have given a total positive rate of 26.16%. The detection rate of the two methods has differed significantly(P<0.05).
  
  
  CONCLUSIONS
  Compared with the R-banding karyotype analysis, the eight-probe FISH is more accurate and efficient. Diagnosis of cytogenetic abnormalities for children with ALL using the combined method can provide a basis for evaluation of prognosis as well as personalized therapy.
authors.affiliation: Genetics Laboratory, Children's Hospital of Zhengzhou, Zhengzhou, Henan 450053, China. 1019231544@qq.com.
authors.forename: Ding
authors.initials: D
authors.lastname: Zhao
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Guo
authors.forename: Rui
authors.initials: R
authors.lastname: Li
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics*