title: Immunoexpression of Ki-67, MCM2, and MCM3 in Ameloblastoma and Ameloblastic Carcinoma and Their Correlations with Clinical and Histopathological Patterns.
abstract: Cell proliferation assays are performed using antibodies against nuclear proteins associated with DNA replication. These nuclear proteins have gained special interest to predict the biological and clinical behaviors of various tumors. The aim of this study was to analyze the presence of Ki-67 protein and the minichromosome maintenance-2 (MCM2) and maintenance-3 (MCM3) proteins in ameloblastoma. Materials and Methods. Cell proliferation marker expression levels were assessed via immunohistochemistry in 111 ameloblastoma cases (72 unicystic ameloblastoma samples, 38 solid/multicystic ameloblastoma samples, and 1 ameloblastic carcinoma). The label index was performed as described previously. Results. MCM2 and MCM3 showed higher proliferation indexes in all variants of ameloblastoma compared to the classic marker Ki-67. No correlation between the proliferation index and the clinical and protein expression data was observed. Conclusion. The results suggest that clinical features do not directly affect tumor cell proliferation. Moreover, the high levels of cellular proliferation of MCM2 and MCM3 compared with Ki-67 may indicate that MCM2 and MCM3 are more sensitive markers for predicting the growth rate and eventually might be helpful as a tool for predicting aggressive and recurrent behaviors in these tumors.
authors.affiliation: Research Department, School of Dentistry, Universidad Juárez del Estado de Durango (UJED), 34000 Durango, DGO, Mexico.
authors.forename: Ramón Gil
authors.initials: RG
authors.lastname: Carreón-Burciaga
authors.affiliation: Research Department, School of Dentistry, Universidad Juárez del Estado de Durango (UJED), 34000 Durango, DGO, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: González-González
authors.affiliation: Health Care Department, Universidad Autónoma Metropolitana, Xochimilco, 04960 Mexico City, DF, Mexico.
authors.forename: Nelly
authors.initials: N
authors.lastname: Molina-Frechero
authors.affiliation: Molecular Pathology Area, School of Dentistry, Universidad de la República (UDELAR), 19200 Montevideo, Uruguay.
authors.forename: Ronell
authors.initials: R
authors.lastname: Bologna-Molina
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000378:metabolism* / Q000473:pathology; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D002648:Child; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2 / Q000378:metabolism*; D064126:Minichromosome Maintenance Complex Component 3 / Q000378:metabolism*
chemical_list: D019394:Ki-67 Antigen; C066470:MCM3 protein, human; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2; D064126:Minichromosome Maintenance Complex Component 3
references.citation: Int J Oral Maxillofac Surg. 2006 Aug;35(8):681-90
references.pmid: 16782308
references.citation: Oral Oncol. 2001 Feb;37(2):193-8
references.pmid: 11167148
references.citation: J Biol Chem. 2007 Nov 16;282(46):33795-804
references.pmid: 17895243
references.citation: Histopathology. 2011 Oct;59(4):801-3
references.pmid: 21939456
references.citation: Pathol Int. 2009 Apr;59(4):229-33
references.pmid: 19351365
references.citation: Oral Dis. 2013 May;19(4):360-5
references.pmid: 22970847
references.citation: Histopathology. 2004 Jul;45(1):65-72
references.pmid: 15228445
references.citation: Med Oral Patol Oral Cir Bucal. 2013 Mar;18(2):e174-9
references.pmid: 23229269
references.citation: Ann Diagn Pathol. 2013 Feb;17(1):32-6
references.pmid: 22652151
references.citation: J Clin Pathol. 1998 Oct;51(10):716-24
references.pmid: 10023332
references.citation: PLoS One. 2015;10(2):e0117241
references.pmid: 25706407
references.citation: Ann Diagn Pathol. 2013 Dec;17(6):518-21
references.pmid: 24090509
references.citation: Cell. 2009 Nov 13;139(4):719-30
references.pmid: 19896182
references.citation: Anticancer Res. 2009 Apr;29(4):1137-42
references.pmid: 19414356
references.citation: Exp Mol Pathol. 2010 Feb;88(1):138-42
references.pmid: 19818763
references.citation: Eur J Cancer B Oral Oncol. 1995 Mar;31B(2):86-99
references.pmid: 7633291
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Feb;95(2):213-21
references.pmid: 12582363
references.citation: Dentomaxillofac Radiol. 2004 May;33(3):141-51
references.pmid: 15371313
references.citation: Med Oral Patol Oral Cir Bucal. 2014 Jan;19(1):e8-e14
references.pmid: 23986011
references.citation: Oral Oncol. 2008 Jan;44(1):50-60
references.pmid: 17307020
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol. 2015 Jan;119(1):83-91
references.pmid: 25446507
references.citation: PLoS One. 2013;8(7):e69607
references.pmid: 23874974
references.citation: Diagn Pathol. 2012;7:14
references.pmid: 22300665
references.citation: Rom J Morphol Embryol. 2014;55(2):363-7
references.pmid: 24969987
references.citation: Biomed Res Int. 2014;2014:549719
references.pmid: 25243149
references.citation: Dis Markers. 2015;2015:301781
references.pmid: 25866434
references.citation: J Oral Pathol Med. 2015 Aug;44(7):543-51
references.pmid: 25243582
references.citation: Mod Pathol. 2002 Mar;15(3):331-41
references.pmid: 11904346
references.citation: J Oral Pathol Med. 2002 Nov;31(10):598-604
references.pmid: 12406305
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Dec;112(6):767-76
references.pmid: 22014999
references.citation: J Pathol. 2014 Apr;232(5):492-8
references.pmid: 24374844
references.citation: Mol Biol Cell. 2007 Apr;18(4):1447-56
references.pmid: 17314407
references.citation: Anticancer Res. 2009 Aug;29(8):3049-52
references.pmid: 19661314
references.citation: J Pathol. 2001 Nov;195(4):457-62
references.pmid: 11745678
references.citation: Anticancer Res. 2014 Aug;34(8):4569-75
references.pmid: 25075101
references.citation: Eur Arch Otorhinolaryngol. 2016 Jul;273(7):1649-61
references.pmid: 25926124
references.citation: Oral Oncol. 2008 Aug;44(8):805-11
references.pmid: 18207448
references.citation: J Oral Pathol Med. 2003 Sep;32(8):468-74
references.pmid: 12901728
references.citation: Oncogene. 2010 Oct 7;29(40):5475-89
references.pmid: 20661220
references.citation: J Oral Pathol Med. 2014 Jul;43(6):427-34
references.pmid: 24456424
references.citation: Oral Dis. 2007 May;13(3):303-7
references.pmid: 17448213
references.citation: J Craniomaxillofac Surg. 2015 Jan;43(1):149-57
references.pmid: 25465490
references.citation: J Cell Biol. 2011 Jan 10;192(1):29-41
references.pmid: 21220507
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Jun;113(6):782-8
references.pmid: 22668706
references.citation: Curr Opin Cell Biol. 2006 Apr;18(2):130-6
references.pmid: 16495042