title: LIPUS promotes spinal fusion coupling proliferation of type H microvessels in bone.
abstract: Low-intensity pulsed ultrasound (LIPUS) has been found to accelerate spinal fusion. Type H microvessels are found in close relation with bone development. We analyzed the role of type H vessels in rat spinal fusion model intervened by LIPUS. It was found LIPUS could significantly accelerate bone fusion rate and enlarge bone callus. Osteoblasts were specifically located on the bone meshwork of the allograft, and were surrounded by type H microvessels. LIPUS could significantly increase the quantity of osteoblasts during spine fusion, which process was coupled with elevated angiogenesis of type H microvessels. Our results suggest that LIPUS may be a noninvasive adjuvant treatment modality in spinal fusion for clinical use. The treatment is recommended for usage for at least one month.
authors.affiliation: Orthopaedic Department of Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Ximing
authors.initials: X
authors.lastname: Xu
authors.affiliation: Orthopaedic Department of Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Orthopaedic Department of Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Orthopaedic Department of Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Orthopaedic Department of Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Xianzhao
authors.initials: X
authors.lastname: Wei
authors.affiliation: Orthopaedic Department of Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D008297:Male; D055806:Microvessels / Q000254:growth & development*; D018919:Neovascularization, Physiologic; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013123:Spinal Fusion; D013131:Spine / Q000098:blood supply* / Q000378:metabolism; D000069453:Ultrasonic Waves
references.citation: Spine (Phila Pa 1976). 1995 Feb 15;20(4):412-20
references.pmid: 7747224
references.citation: Ultrasound Med Biol. 2012 Dec;38(12):2120-9
references.pmid: 23062367
references.citation: Eur Spine J. 2001 Oct;10 Suppl 2:S141-6
references.pmid: 11716011
references.citation: J Ultrasound Med. 2003 Feb;22(2):145-53
references.pmid: 12562119
references.citation: Spine J. 2007 Sep-Oct;7(5):609-14
references.pmid: 17526434
references.citation: Clin Orthop Relat Res. 2003 Feb;(407):102-18
references.pmid: 12567137
references.citation: J Bone Joint Surg Am. 2008 May;90(5):1043-52
references.pmid: 18451397
references.citation: Eur Spine J. 2004 Jul;13(4):354-8
references.pmid: 14730438
references.citation: Tissue Eng Part A. 2010 Dec;16(12):3679-86
references.pmid: 20618082
references.citation: Eur Spine J. 1999;8(4):284-9
references.pmid: 10483830
references.citation: J Bone Miner Res. 2001 Apr;16(4):671-80
references.pmid: 11315994
references.citation: Spine (Phila Pa 1976). 2000 Sep 15;25(18):2400-2
references.pmid: 10984795
references.citation: Spine (Phila Pa 1976). 2007 Dec 1;32(25):2885-90
references.pmid: 18246013
references.citation: J Child Orthop. 2013 Feb;7(1):57-62
references.pmid: 24432060
references.citation: Eur Spine J. 2014 Feb;23(2):426-34
references.pmid: 24166021
references.citation: Spine (Phila Pa 1976). 2010 Dec 15;35(26):E1539-45
references.pmid: 20714282
references.citation: Spine (Phila Pa 1976). 2004 Jun 15;29(12):1308-13
references.pmid: 15187630
references.citation: Spine J. 2013 Oct;13(10):1273-80
references.pmid: 23953506
references.citation: J Bone Joint Surg Am. 1994 Jan;76(1):26-34
references.pmid: 8288661
references.citation: J Ther Ultrasound. 2014 Jan 02;2:1
references.pmid: 25516803
references.citation: Bone. 2011 Sep;49(3):447-54
references.pmid: 21658484
references.citation: Spine (Phila Pa 1976). 2012 Feb 1;37(3):E139-46
references.pmid: 21738092
references.citation: Spine (Phila Pa 1976). 2011 Jun;36(13):1010-6
references.pmid: 21325987
references.citation: Nature. 2014 Mar 20;507(7492):323-8
references.pmid: 24646994
references.citation: Bone. 2004 Jul;35(1):114-23
references.pmid: 15207747
references.citation: FEBS Lett. 2015 Jan 30;589(3):310-8
references.pmid: 25542352
references.citation: Eur Spine J. 2010 Dec;19(12):2200-8
references.pmid: 20429017
references.citation: JAMA. 2009 Jul 1;302(1):58-66
references.pmid: 19567440
references.citation: Spine (Phila Pa 1976). 2003 Jul 1;28(13):1390-5
references.pmid: 12838096
references.citation: Spine J. 2001 Jul-Aug;1(4):246-54
references.pmid: 14588328
references.citation: Oncol Rep. 2012 Aug;28(2):481-6
references.pmid: 22614439
references.citation: Ultrasound Med Biol. 2007 Nov;33(11):1818-29
references.pmid: 17681676
references.citation: Nature. 2014 Mar 20;507(7492):376-80
references.pmid: 24647000
references.citation: Injury. 2015 Oct;46(10):2036-41
references.pmid: 26052056
references.citation: Spine (Phila Pa 1976). 2002 Dec 1;27(23):2662-73
references.pmid: 12461392
references.citation: Calcif Tissue Int. 1998 Oct;63(4):357-60
references.pmid: 9744997
references.citation: Spine J. 2015 Apr 1;15(4):692-9
references.pmid: 25523380
references.citation: J Bone Joint Surg Am. 1997 Jul;79(7):961-73
references.pmid: 9234872
references.citation: Ultrasound Med Biol. 2004 Mar;30(3):389-95
references.pmid: 15063521
references.citation: Am J Pathol. 2009 Jun;174(6):2160-71
references.pmid: 19389927
references.citation: BMC Musculoskelet Disord. 2010;11:229
references.pmid: 20932272
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9656-61
references.pmid: 12118119
references.citation: Spine (Phila Pa 1976). 1995 May 1;20(9):1055-60
references.pmid: 7631235
references.citation: Pediatr Clin North Am. 2014 Dec;61(6):1223-41
references.pmid: 25439021
references.citation: Ultrasound Med Biol. 2008 Aug;34(8):1248-60
references.pmid: 18378382
references.citation: Spine (Phila Pa 1976). 1999 Apr 1;24(7):637-45
references.pmid: 10209791
references.citation: Spine (Phila Pa 1976). 2004 Apr 1;29(7):726-33; discussion 733-4
references.pmid: 15087793
references.citation: Clin Orthop Relat Res. 2015 May;473(5):1830-9
references.pmid: 25736917
references.citation: Bone. 2008 Aug;43(2):348-54
references.pmid: 18538648
references.citation: Spine (Phila Pa 1976). 2005 May 1;30(9):1001-7; discussion 1007
references.pmid: 15864149