title: A comparative study of the amount of α-synuclein in ischemic stroke and Parkinson's disease.
abstract: In the present study, we detected the level of oligomeric form of α-synuclein in the red blood cells of ischemic stroke, Parkinson's disease, and normal people and compared the differences to assess the diagnosis potential of α-synuclein in ischemic stroke patients. 86 ischemic stroke, 100 PD, and 102 healthy cases were enrolled in the present study. Total protein amount in the red blood cells were quantified by BCA assay using spectrophotometer. Levels of oligomeric form of α-synuclein were characterized by a sandwich ELISA. Analysis of correlation analysis and receiver operating characteristic curve were conducted. Significant differences were detected in the levels of oligomeric forms of α-synuclein in different samples' blood cells (P < 0.05); the levels of total protein in (188.1 ± 33.9 mmol/L) healthy people were significantly higher than that of PD (147.7 ± 45.0 mmol/L) and ischemic stroke groups (142.9 ± 43.0 mmol/L) (P < 0.05). There was no correlation between the age of patients and level of α-synuclein (R (2) = 0.216 in ischemic stroke group and -0.104 in PD group) and the receiver operating characteristic curve analysis showed a high sensitivity of α-synuclein in discriminating ischemic stroke (sensitivity was 63.7 % and specificity was 9.6 %) and PD (sensitivity was 44.1 % and specificity was 12.5 %) patients from the controls. The levels of oligomeric form of α-synuclein of red blood cells in ischemic stroke and Parkinson's disease patients were both significant higher than normal people. And the level of oligomeric form α-synuclein showed a potential for diagnosis of ischemic stroke in clinic.
authors.affiliation: Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, #6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Zhao
authors.affiliation: Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, #6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Fang-Fei
authors.initials: FF
authors.lastname: Li
authors.affiliation: Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, #6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, #6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing, 100050, China. happyft@sina.com.|China National Clinical Research Center for Neurological Diseases, #6 Tiantan Xili, Dongcheng District, Beijing, 100050, China. happyft@sina.com.|Parkinson's Disease Center, Beijing Institute for Brain Disorders, Capital Medical University, No.45 Changchun Street, Beijing, 100053, China. happyft@sina.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D002545:Brain Ischemia / Q000097:blood* / Q000150:complications; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000097:blood*; D012372:ROC Curve; D020521:Stroke / Q000097:blood* / Q000209:etiology; D055815:Young Adult; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D051844:alpha-Synuclein
keywords: Ischemice stroke; Parkinson’ disease; Red blood cells; α-Synuclein
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