title: Stimulation of Early Osteochondral Differentiation of Human Mesenchymal Stem Cells Using Binary Colloidal Crystals (BCCs).
abstract: A new surface based on self-assembly of two colloids into well-defined nanostructures, so-called binary colloidal crystals (BCCs), was fabricated for stem cell culture. The facile fabrication process are able to cover large surface areas (>3 cm-diameter, i.e. > 7 cm(2)) with ordered surface nanotopographies that is often a challenge particularly in biomaterials science. From our library, four different combinations of BCCs were selected using mixtures of silica, polystyrene and poly(methyl methacrylate) particles with sizes in the range from 100 nm to 5 μm. Cell spreading, proliferation, and surface-induced lineage commitment of human adipose-derived stem cells (hADSCs) was studied using quantitative real time polymerase chain reaction (qRT-PCR) and immunostaining. The results showed that BCCs induced osteo- and chondro- but not adipo-gene expression in the absence of induction medium suggesting that the osteochondral lineage can be stimulated by the BCCs. When applying induction media, higher osteo- and chondro-gene expression on BCCs was found compared with tissue culture polystyrene (TCPS) and flat silica (Si) controls, respectively. Colony forming of chondrogenic hADSCs was found on BCCs and TCPS but not Si controls, suggesting that the differentiation of stem cells is surface-dependent. BCCs provide access to complex nanotopographies and chemistries, which can find applications in cell culture and regenerative medicine.
authors.affiliation: Department of Chemistry and Biotechnology, Swinburne University of Technology , Hawthorn, 3122 Victoria, Australia.|CSIRO Manufacturing , Bayview Avenue, Clayton, 3168 Victoria, Australia.|Department of Anatomy and Neuroscience, Florey Neuroscience and Mental Health Institute, The University of Melbourne , Melbourne, Australia.
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Wang
authors.affiliation: CSIRO Manufacturing , Bayview Avenue, Clayton, 3168 Victoria, Australia.
authors.forename: Helmut
authors.initials: H
authors.lastname: Thissen
authors.affiliation: Department of Chemistry and Biotechnology, Swinburne University of Technology , Hawthorn, 3122 Victoria, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Kingshott
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D020219:Chondrogenesis / Q000187:drug effects*; D003102:Colloids / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D050866:Liquid Crystals / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects*; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D003102:Colloids
keywords: adipogenesis; binary colloidal crystals; chondrogenesis; human stem cells; osteogenesis