title: Morphometry As a Diagnostic Tool for Potentially Malignant Lesions.
abstract: BACKGROUND
  Though we are in 21(st) century with nano technology & tissue printing, there still exist many lacunae in the field of diagnosis. Not much is known about prognostic markers till now from literature to assess potentially malignant lesions. Lesions so called potentially malignant can be termed only after clinical & malignant changes have been developed and there are no means of predicting with certainty the risk of cancerous transformation.
  
  
  OBJECTIVE
  Our present study was undertaken to establish the morphometric parameters of the parabasal and spinous cells of normal oral epithelium with the changes occurring in cells of Oral Leukoplakia (OL), Oral Verrucous Carcinoma (OVC) and Oral Squamous Cell Carcinoma (OSCC).
  
  
  METHODS
  Total 40 patients were divided into Group I which includes patients with normal oral mucosa, group II oral leukoplakia patients, group III oral verrucous carcinoma patients and group IV includes oral squamous cell carcinoma patients. Tissue sections were taken and morphometric analysis of cell area, cell diameter, nuclear area, nuclear diameter, nuclear cytoplasmic ratio was done for parabasal and spinous layer cells. Statistical analysis was done using one-way ANOVA and T-test.
  
  
  RESULTS
  Nuclear diameter, nuclear area, cell area, nuclear cytoplasmic ratio were significantly increased in OL, OVC, OSCC patients than normal oral mucosa, which was statistically significant. Cell diameter was decreased in OL, OVC, OSCC patients than with normal oral mucosa which was statistically significant.
  
  
  CONCLUSIONS
  Cellular & nuclear parameters showed statistically significant changes in oral leukoplakia, oral verrucous carcinoma & oral squamous cell carcinoma in comparison with normal oral mucosa.
authors.affiliation: Research Scholar, Stem Cell Laboratory, Narayan Nethralaya , Bommasandra, Bangalore, Karnataka, India .
authors.forename: Vineetha
authors.initials: V
authors.lastname: Christopher
authors.affiliation: Professor and HOD, Department of Oral Pathology and Microbiology, Sri Hasanamba Dental College and Hospital , Hassan, Karnataka, India .
authors.forename: Sarvani
authors.initials: S
authors.lastname: Murthy
authors.affiliation: Reader, Department of Oral Medicine and Radiology, School of Dental Sciences, KIMSDU , Karad, Maharashtra, India .
authors.forename: Ashwinirani
authors.initials: A
authors.lastname: Sr
authors.affiliation: Post Graduate Student, Department of Oral Pathology and Microbiology, Sri Hasanamba Dental College and Hospital , Hassan, Karnataka, India .
authors.forename: Sakshi
authors.initials: S
authors.lastname: Singh
authors.affiliation: Post Graduate Student, Department of Oral Pathology and Microbiology, Sri Hasanamba Dental College and Hospital , Hassan, Karnataka, India .
authors.forename: Athira
authors.initials: A
authors.lastname: Cp
authors.affiliation: Post Graduate Student, Department of Oral Pathology and Microbiology, Sri Hasanamba Dental College and Hospital , Hassan, Karnataka, India.
authors.forename: Shilpa Kuppareddy
authors.initials: SK
authors.lastname: Shivaram
authors.affiliation: Post Graduate Student, Department of Oral Pathology and Microbiology, Sri Hasanamba Dental College and Hospital , Hassan, Karnataka, India .
authors.lastname: Neethupriya
keywords: Caliper-Pro 4.2; Image analyser; Oral Precancer
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