title: Electrospun Nanofibrous Sheets for Selective Cell Capturing in Continuous Flow in Microchannels.
abstract: Electrospun nanofibrous meshes were surface-modified for selective capturing of specific cells from a continuous flow in PDMS microchannels. We electrospun nanofibrous mats composed of poly(ε-carprolactone) (PCL) and amine-functionalized block copolymers composed of PCL and poly(ethylenimine) (PEI). A mixture of biotinylated PEG and blunt PEG was chemically tethered to the nanofibrous mats via the surface-exposed amines on the mat. The degree of biotinylation was fluorescently and quantitatively assayed for confirming the surface-biotinylation levels for avidin-specific binding. The incorporation level of avidin gradually increased when the blend ratio of biotinylated PEG on the mat increased, confirming the manipulated surfaces with various degree of biotinylation. Biotinylated cells were incubated with avidin-coated biotinylated mats and the specific binding of biotinylated cells was monitored in a microfluidic channel with a continuous flow of culture medium, which suggests efficient and selective capturing of the biotinylated cells on the nanofibrous mat.
authors.affiliation: Department of Medical Biomaterials Engineering and §Institute of Bioscience and Biotechnology, Kangwon National University , Chuncheon 200-701, Republic of Korea.
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Son
authors.affiliation: Department of Medical Biomaterials Engineering and §Institute of Bioscience and Biotechnology, Kangwon National University , Chuncheon 200-701, Republic of Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Medical Biomaterials Engineering and §Institute of Bioscience and Biotechnology, Kangwon National University , Chuncheon 200-701, Republic of Korea.
authors.forename: Hye Sung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Medical Biomaterials Engineering and §Institute of Bioscience and Biotechnology, Kangwon National University , Chuncheon 200-701, Republic of Korea.
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001360:Avidin / Q000378:metabolism; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D011091:Polyesters; D001360:Avidin; C016240:polycaprolactone; D011092:Polyethylene Glycols; D001710:Biotin