title: High expression of N-acetyltransferase 10: a novel independent prognostic marker of worse outcome in patients with hepatocellular carcinoma.
abstract: N-acetyltransferase 10 (NAT10) is a nucleolar protein involved in histone acetylation, telomerase activity regulation, DNA damage response and cytokinesis. The expression of NAT10 was found to be enhanced in several types of tumors, suggesting its correlation with tumor development. However, the specific role of NAT10 in hepatocellular carcinoma (HCC) is still unclear. The aim of this study was to investigate the expression of NAT10 in HCC patients and to assess the relationship of NAT10 expression with clinicopathological characteristics and tumor prognosis. We selected 17 pairs of HCC samples and adjacent non-neoplastic tissue for mRNA expression analysis. We also performed immunohistochemistry in 186 HCC samples to evaluate the NAT10 protein expression. Cox regression and Kaplan-Meier analysis was used to study the diagnostic and prognostic value of NAT10. The results showed that NAT10 expression was mainly localized in the nuclei/nucleoli and was significantly higher in HCC tissues than peritumoral tissues (P < 0.01). High NAT10 expression was positively correlated with histological differentiation (P < 0.01) and TNM classification (P < 0.01). Cox regression univariate and multivariable analysis revealed that expression of NAT10 in HCC was an independent prognostic factor for patient survival time. Our data suggested that NAT10 might be a promising prognostic marker and potential therapeutic target in HCC.
authors.affiliation: Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine Hangzhou 310003, China.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The First Affiliated Hospital, Zhejiang University School of MedicineHangzhou 310003, China; Department of Pathology and Molecular Medicine, Faculty of Health Science, McMaster UniversityHamilton, Ontario, Canada L8S 4K1.
authors.forename: Jimin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University Hangzhou 310003, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine Hangzhou 310003, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine Hangzhou 310003, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityHangzhou 310003, China; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public HealthHangzhou 310003, China; Key Laboratory of Organ TransplantationHangzhou 310003, China.
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000201:enzymology / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D063213:N-Terminal Acetyltransferase E / Q000032:analysis / Q000096:biosynthesis*; D063206:N-Terminal Acetyltransferases; D011379:Prognosis; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D063213:N-Terminal Acetyltransferase E; D063206:N-Terminal Acetyltransferases; C522989:NAT10 protein, human
keywords: NAT10; hepatocellular carcinoma; prognosis
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