title: Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection.
abstract: Griffithsin (GRFT) is a broad-spectrum antiviral protein against several glycosylated viruses. In our previous publication, we have shown that GRFT exerted antiviral activity against Japanese encephalitis virus (JEV) infection. Herein, we further elucidated the mechanism by which GRFT inhibits JEV infection in BHK-21 cells. In vitro experiments using Pull-down assay and Co-immunoprecipitation (CO-IP) assay showed that GRFT binds to the JEV glycosylated viral proteins, specifically the enveloped (E) and premature (prM) glycoproteins. The binding of GRFT to the JEV was competitively inhibited by increasing concentrations of mannose; in turns abolished anti-JEV activity of GRFT. We suggested that, the binding of GRFT to the glycosylated viral proteins may contribute to its anti-JEV activity. Collectively, our data indicated a possible mechanism by which GRFT exerted its anti-JEV activity. This observation suggests GRFT's potentials in the development of therapeutics against JEV or other flavivirus infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China; College of Veterinary Sciences, Nyala University, Nyala, Sudan. Electronic address: hassan8377@yahoo.com.
authors.forename: Hassan Z A
authors.initials: HZ
authors.lastname: Ishag
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China. Electronic address: xmao@njau.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D002460:Cell Line; D002498:Centrifugation; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000187:drug effects*; D006023:Glycoproteins / Q000378:metabolism*; D047468:Immunoprecipitation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D037121:Plant Lectins / Q000378:metabolism*; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D037121:Plant Lectins; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus; C074932:prM protein, Flavivirus; C499654:griffithsin protein, Griffithsia
keywords: Glycosylated proteins; Griffithsin; Inhibition; Japanese encephalitis virus
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