title: Knockdown of WISP1 inhibit proliferation and induce apoptosis in ALL Jurkat cells.
abstract: WISP1, a Wnt-induced secreted protein, has been found to have anticancer activity. ALL is a leading cause of death. Here we investigate the WISP1 effects on ALL Jurkat cells. Cell viability was assessed by CCK-8. Cell cycle and apoptosis were detected by flow cytometry. Mitochondrial membrane potential (MMP) was monitored using TMRM. Generation of reactive oxygen species (ROS) was quantified using DCFH-DA. Western blot was used to detect the expression of cell proliferation and apoptosis related genes. The results showed that knockdown of WISP1 significantly inhibited proliferation of Jurkat cells. Parallelly, cell cycle distribution was increased at G1 phase and apoptotic rate was induced after WISP1 knockdown. Furthermore, knockdown of WISP1 induced apoptosis of Jurkat cells was also associated with loss of MMP and generation of ROS. Western blot results showed that the protein expression p-AKT, PCNA, CDK1, P-ERK, CDK2, VEGF, VEGFR2 and Bcl2 were decreased, while the expression of Bax was up-regulated. In conclusion, WISP1 plays an important role in proliferation and apoptosis of Jurkat cells in mitochondria dependent pathway, the specific mechanisms need further study.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Xiushuai
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yinglan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D055511:CCN Intercellular Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation; D015536:Down-Regulation; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D020298:U937 Cells
chemical_list: D051017:Apoptosis Regulatory Proteins; D055511:CCN Intercellular Signaling Proteins; C116396:CCN4 protein, human; D018797:Cell Cycle Proteins; D011518:Proto-Oncogene Proteins; D017382:Reactive Oxygen Species
keywords: ALL; MMP; ROS; WISP1; apoptosis; proliferation
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