title: MiR-145 suppressed human retinoblastoma cell proliferation and invasion by targeting ADAM19.
abstract: MicroRNAs (miRNAs) play critical roles in retinoblastoma (RB) initiation and progression, aberrant expression of miR-145 had been frequently reported in cancer studies. However, the role and mechanism of its function in RB is still unclear. In this study, our data showed that miR-145 was downregulated in RB tissues and cell lines. Overexpression of miR-145 suppressed RB cell proliferation, migration and invasion in vitro. ADAM19 was identified as a direct target of miR-145. Silencing of ADAM19 significantly inhibited RB cell proliferation, migration and invasion. In addition, a reverse correlation between miR-145 and ADAM19 expression was noted in RB tissues. Taken together, these findings suggested that miR-145 functions as a tumor suppressor in RB by directly targeting ADAM19. miR-145 could be an anticancer therapeutic target for RB patients.
authors.affiliation: School of Medicine, Tongji UniversityShanghai 200092, China; Department of Ophthalmology, The Affiliated Hospital of Qingdao UniversityQingdao 266000, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Qingdao Women and Children's Hospital, Qingdao University Qinddao 266000, China.
authors.forename: Ai
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, The Affiliated Hospital of Qingdao University Qingdao 266000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, The Affiliated Hospital of Qingdao University Qingdao 266000, China.
authors.forename: Zhaodong
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Ophthalmology, The Affiliated Hospital of Qingdao University Qingdao 266000, China.
authors.forename: Chengye
authors.initials: C
authors.lastname: Che
authors.affiliation: School of Medicine, Tongji University Shanghai 200092, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000235:genetics* / Q000473:pathology; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D051722:ADAM Proteins; C411902:ADAM19 protein, human
keywords: ADAM19; Retinoblastoma; miR-145
references.citation: Nat Rev Genet. 2010 Sep;11(9):597-610
references.pmid: 20661255
references.citation: Carcinogenesis. 2014 Feb;35(2):469-78
references.pmid: 24085799
references.citation: Biochem Biophys Res Commun. 2015 Sep 18;465(2):262-9
references.pmid: 26255969
references.citation: Curr Opin Cell Biol. 1998 Oct;10(5):654-9
references.pmid: 9818177
references.citation: Oncol Rep. 2014 Jul;32(1):261-9
references.pmid: 24807198
references.citation: PLoS One. 2015;10(3):e0120698
references.pmid: 25799050
references.citation: FEBS Lett. 2013 Nov 15;587(22):3729-37
references.pmid: 24140344
references.citation: Nat Rev Mol Cell Biol. 2012 Apr;13(4):239-50
references.pmid: 22436747
references.citation: Biochem Biophys Res Commun. 2015 Jan 2;456(1):385-91
references.pmid: 25475731
references.citation: Int J Clin Exp Med. 2015 Jun 15;8(6):8794-802
references.pmid: 26309531
references.citation: Nature. 2014 Oct 16;514(7522):312-3
references.pmid: 25252972
references.citation: Tumour Biol. 2014 Sep;35(9):8933-7
references.pmid: 24894676
references.citation: FEBS J. 2014 Mar;281(5):1355-65
references.pmid: 24289859
references.citation: Cancer Invest. 2015 Jul;33(6):251-8
references.pmid: 25951106
references.citation: Circ Cardiovasc Genet. 2014 Jun;7(3):350-8
references.pmid: 24951661
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: FEBS Lett. 2015 Jan 30;589(3):407-13
references.pmid: 25554419
references.citation: Childs Nerv Syst. 2009 Jan;25(1):13-20
references.pmid: 18818933
references.citation: Dev Biol. 2007 Feb 1;302(1):1-12
references.pmid: 16989803
references.citation: CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90
references.pmid: 21296855
references.citation: Lancet. 2012 Apr 14;379(9824):1436-46
references.pmid: 22414599