title: Microglial polarization dynamics in dorsal spinal cord in the early stages following chronic sciatic nerve damage.
abstract: Peripheral nerve injury can lead to activation of spinal microglia, which can mediate neuroinflammation and contribute to neuropathic pain following nerve injury. Activated microglia may manifest with either pro-inflammatory M1 phenotype or anti-inflammatory M2 phenotype, which may lead to detrimental or beneficial roles in the nervous system. In this study, microglia numbers, morphology and gene profiles were examined in the dorsal spinal cord of rats over 14 days following sciatic nerve chronic constriction injury (CCI). The morphology of some microglia changed from a surveying to an activated state within 1 day of CCI. Neuropathic pain developed within seven to 14 days following injury and microglia numbers were increased, with almost all in the dorsal spinal cord morphologically defined as activated. At day one after CCI, both M1 and M2 microglia-related genes were increased but only M1 microglia-related genes remained elevated at day seven and 14 thereafter. These results indicate that both M1 and M2 microglia were activated in the dorsal spinal cord one day after CCI but the microglia skewed towards M1 phenotype during the following seven and 14 days.
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Fangting
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Breast Surgery, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Zongbin
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Qulian
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha 410008, China. Electronic address: docthuang2005@hotmail.com.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D016764:Cell Polarity; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism; D006930:Hyperalgesia / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D017628:Microglia / Q000502:physiology*; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D010812:Physical Stimulation; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries*; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology*
chemical_list: D016207:Cytokines
keywords: M1/M2 phenotype; Microglia; Nerve injury; Neuropathic pain