title: Saligenin cyclic-o-tolyl phosphate (SCOTP) induces autophagy of rat spermatogonial stem cells.
abstract: Tri-ortho-cresyl phosphate (TOCP) has been widely used as plasticizers, plastic softeners, and flame-retardants in industry, which can be metabolized to High-toxic saligenin cyclic-o-tolyl phosphate (SCOTP). Our previous results found that TOCP could disrupt the seminiferous epithelium in the testis and induce autophagy of rat spermatogonial stem cells. Little is known about the toxic effect of SCOTP on rat spermatogonial stem cells. The present study showed that SCOTP decreased viability of rat spermatogonial stem cells in a dose-dependent manner. Both LC3-II and the ratio of LC3-II/LC3-I were significantly increased; autophagy proteins atg5 and Beclin 1 were also markedly increased after treatment with SCOTP, indicating SCOTP could induce autophagy of the cells. Ultrastructural observation under the transmission electron microscopy (TEM) indicated that there were autophagic vacuoles in the cytoplasm in the SCOTP-treated cells. However, cell cycle arrest was not observed by flow cytometry; and the mRNA levels of p21, p27, p53 and cyclin D1 in the cells were also not affected by SCOTP. Meanwhile, SCOTP didn't induce apoptosis of the cells. In summary, we showed that SCOTP could induce autophagy of rat spermatogonial stem cells, without affecting cell cycle and apoptosis.
authors.affiliation: Medical Research Center, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China; Nursing school of Jiujiang University, Jiujiang 332000, PR China.
authors.forename: Meng-Ling
authors.initials: ML
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Jing-Lei
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Library, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China. Electronic address: chenjiaxiang@ncu.edu.cn.
authors.forename: Jia-Xiang
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000072956:Adult Germline Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects; D000071187:Autophagy-Related Protein 5 / Q000378:metabolism; D000071186:Beclin-1 / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D008297:Male; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010968:Plasticizers / Q000633:toxicity*; D051381:Rats
chemical_list: D000071187:Autophagy-Related Protein 5; D000071186:Beclin-1; D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D009943:Organophosphorus Compounds; D010968:Plasticizers; C495773:Atg5 protein, mouse; C115010:Becn1 protein, mouse; C526109:Anxa3 protein, rat; C000516:2-(2-cresyl)-4H-1-3-2-benzodioxaphosphorin-2-oxide
keywords: Autophagy; Saligenin cyclic-o-tolyl phosphate; Spermatogonial stem cells