title: Bioconjugating Thiols to Poly(acrylamide) Gels for Cell Culture Using Methylsulfonyl Co-monomers.
abstract: Poly(acrylamide) P(AAm) gels have become relevant model substrates to study cell response to the mechanical and biochemical properties of the cellular microenvironment. However, current bioconjugation strategies to functionalize P(AAm) gels, mainly using photoinduced arylazide coupling, show poor specificity and hinder conclusive studies of material properties and cellular responses. We describe methylsulfonyl-containing P(AAm) hydrogels for cell culture. These gels allow easy, specific and functional covalent coupling of thiol containing bioligands in tunable concentrations under physiological conditions, while retaining the same swelling, porosity, cytocompatibility, and low protein adsorption of P(AAm) gels. These materials allow quantitative and standardized studies of cell-materials interactions with P(AAm) gels.
authors.affiliation: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128, Mainz, Germany.|INM-Leibniz Institute for New Materials., Campus D2 2, 66123, Saarbrücken, Germany.
authors.forename: Aleeza
authors.initials: A
authors.lastname: Farrukh
authors.affiliation: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128, Mainz, Germany.|INM-Leibniz Institute for New Materials., Campus D2 2, 66123, Saarbrücken, Germany.
authors.forename: Julieta I
authors.initials: JI
authors.lastname: Paez
authors.affiliation: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128, Mainz, Germany.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Salierno
authors.affiliation: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128, Mainz, Germany. aranzazu.delcampo@leibniz-inm.de.|INM-Leibniz Institute for New Materials., Campus D2 2, 66123, Saarbrücken, Germany. aranzazu.delcampo@leibniz-inm.de.|Saarland University, Campus Saarbrücken D2 2, 66123, Saarbrücken, Germany. aranzazu.delcampo@leibniz-inm.de.
authors.forename: Aránzazu
authors.initials: A
authors.lastname: del Campo
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D018929:Cell Culture Techniques; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry*; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D020100:Hydrogels; D013438:Sulfhydryl Compounds; D000099166:Polyacrylamides
keywords: cell culture; chemoselectivity; poly(acrylamide) gels; protein coupling; thiol bioconjugation