title: The Zinc-Finger Protein SOP1 Is Required for a Subset of the Nuclear Exosome Functions in Arabidopsis.
abstract: Correct gene expression requires tight RNA quality control both at transcriptional and post-transcriptional levels. Using a splicing-defective allele of PASTICCINO2 (PAS2), a gene essential for plant development, we isolated suppressor mutations modifying pas2-1 mRNA profiles and restoring wild-type growth. Three suppressor of pas2 (sop) mutations modified the degradation of mis-spliced pas2-1 mRNA species, allowing the synthesis of a functional protein. Cloning of the suppressor mutations identified the core subunit of the exosome SOP2/RRP4, the exosome nucleoplasmic cofactor SOP3/HEN2 and a novel zinc-finger protein SOP1 that colocalizes with HEN2 in nucleoplasmic foci. The three SOP proteins counteract post-transcriptional (trans)gene silencing (PTGS), which suggests that they all act in RNA quality control. In addition, sop1 mutants accumulate some, but not all of the misprocessed mRNAs and other types of RNAs that are observed in exosome mutants. Taken together, our data show that SOP1 is a new component of nuclear RNA surveillance that is required for the degradation of a specific subset of nuclear exosome targets.
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
authors.forename: Kian
authors.initials: K
authors.lastname: Hématy
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bellec
authors.affiliation: Center for Genomics and Bioinformatics, Indiana University, Bloomington, Indiana, United States of America.|School of Informatics and Computing, Indiana University, Bloomington, Indiana, United States of America.
authors.forename: Ram
authors.initials: R
authors.lastname: Podicheti
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bouteiller
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.|Univ Paris-Sud, Université Paris-Saclay, Orsay, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Anne
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.|Univ Paris-Sud, Université Paris-Saclay, Orsay, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Morineau
authors.affiliation: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts, United Kingdom.
authors.forename: Richard P
authors.initials: RP
authors.lastname: Haslam
authors.affiliation: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts, United Kingdom.
authors.forename: Frederic
authors.initials: F
authors.lastname: Beaudoin
authors.affiliation: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts, United Kingdom.
authors.forename: Johnathan A
authors.initials: JA
authors.lastname: Napier
authors.affiliation: Department of Biology, Indiana University, Bloomington, Indiana, United States of America.|Pervasive Technology Institute, Indiana University, Bloomington, Indiana, United States of America.
authors.forename: Keithanne
authors.initials: K
authors.lastname: Mockaitis
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, Strasbourg, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Gagliardi
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Vaucheret
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, Strasbourg, France.
authors.forename: Heike
authors.initials: H
authors.lastname: Lange
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Faure
mesh_terms: D000483:Alleles; D017398:Alternative Splicing / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D055354:Exosomes / Q000378:metabolism*; D016153:Genes, Suppressor; D056426:Genetic Loci; D007438:Introns / Q000235:genetics; D009154:Mutation / Q000235:genetics; D059365:Nonsense Mediated mRNA Decay; D009687:Nuclear Proteins / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics; D016335:Zinc Fingers; D009004:Monosaccharide Transport Proteins
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D022821:RNA Splice Sites; C435922:At1g10760 protein, Arabidopsis; D009004:Monosaccharide Transport Proteins
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