title: miR-130b-3p Upregulation Contributes to the Development of Thyroid Adenomas Targeting CCDC6 Gene.
abstract: We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR-130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified CCDC6, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity.
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale-CNR, Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Leone
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale-CNR, Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Langella
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale-CNR, Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Esposito
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale-CNR, Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: De Martino
authors.affiliation: Department of Pathology, Lyon Sud Hospital Center, Hospices Civils de Lyon, Lyon, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Decaussin-Petrucci
authors.affiliation: Istituto Nazionale per lo Studio e la Cura dei Tumori 'Fondazione Giovanni Pascale', IRCCS, Naples, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Chiappetta
authors.affiliation: Dipartimento di Sanità Pubblica, Università di Napoli Federico II, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bianco
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale-CNR, Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy; Instituto Nacional de Cancer (INCA), Rio de Janeiro, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
keywords: Adenoma; Cell cycle; MicroRNA; Thyroid
references.citation: Cancer Res. 2009 Mar 1;69(5):1844-50
references.pmid: 19223528
references.citation: Nat Cell Biol. 2006 Mar;8(3):278-84
references.pmid: 16489342
references.citation: Nat Rev Mol Cell Biol. 2008 Mar;9(3):219-30
references.pmid: 18270516
references.citation: Nat Rev Mol Cell Biol. 2005 May;6(5):376-85
references.pmid: 15852042
references.citation: Mol Carcinog. 2015 Nov;54(11):1292-300
references.pmid: 25154741
references.citation: Proc Natl Acad Sci U S A. 1980 Jun;77(6):3455-9
references.pmid: 6106191
references.citation: Mol Cell Biol. 1987 Sep;7(9):3365-70
references.pmid: 3670314
references.citation: Mol Cell Endocrinol. 2014 Jun 5;390(1-2):1-7
references.pmid: 24681352
references.citation: Nature. 2004 Sep 16;431(7006):350-5
references.pmid: 15372042
references.citation: BMC Cancer. 2015 Jan 31;15:29
references.pmid: 25637514
references.citation: Oncogene. 2004 Jan 8;23(1):109-21
references.pmid: 14712216
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: Mol Cancer. 2015 Feb 10;14:36
references.pmid: 25888956
references.citation: J Clin Endocrinol Metab. 2012 Sep;97(9):3292-301
references.pmid: 22730517
references.citation: Neoplasia. 2013 Sep;15(9):1086-99
references.pmid: 24027433
references.citation: Mol Endocrinol. 2011 Oct;25(10):1819-30
references.pmid: 21816899
references.citation: Br J Cancer. 2007;96 Suppl:R40-4
references.pmid: 17393584
references.citation: Cell. 1990 Feb 23;60(4):557-63
references.pmid: 2406025
references.citation: Science. 2004 Jan 2;303(5654):83-6
references.pmid: 14657504
references.citation: PLoS One. 2013 Sep 10;8(9):e73803
references.pmid: 24040078
references.citation: Oncotarget. 2015 Jun 20;6(17):15628-38
references.pmid: 25970781
references.citation: Cell. 2005 Jan 14;120(1):15-20
references.pmid: 15652477
references.citation: J Mol Endocrinol. 2014 Mar 06;52(2):181-9
references.pmid: 24443580
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: PLoS One. 2014 May 09;9(5):e96472
references.pmid: 24816756
references.citation: Oncogene. 2010 Jul 29;29(30):4341-51
references.pmid: 20498639
references.citation: Immunity. 2007 Feb;26(2):133-7
references.pmid: 17307699