title: Effects of Acute Stroke Serum on Non-Ischemic Cerebral and Mesenteric Vascular Function.
abstract: We investigated the effects of circulating factors in serum obtained from patients in the acute phase of different subtypes of ischemic stroke on non-ischemic cerebral and mesenteric arteries, as a potential mechanism involved in influencing regional perfusion and thus clinical evolution. Posterior cerebral arteries (PCAs) and mesentery arteries (MAs) isolated from Wistar Kyoto rats were perfused with serum from acute stroke patients with large vessel disease without (LVD) or with hypertension (LVD + HTN), cardioembolism with hypertension (CE + HTN), or physiologic saline as controls. Myogenic activity and nitric oxide-dependent vasorelaxation were assessed after 2 h of intraluminal exposure to serum. Vascular function was differentially affected by sera. Exposure to LVD serum increased myogenic tone and produced endothelial dysfunction in both PCAs and MAs. However, CE + HTN serum increased tone and decreased smooth muscle sensitivity to NO in vessels from both vascular beds. LVD + HTN serum was associated with reduced smooth muscle sensitivity to NO in vessels from both vascular beds but increased tone only in PCAs. Inflammation and oxidative stress, determined by measurement of high sensitivity C-reactive protein, uric acid, and free 8-isoprostane, were enhanced in all the serum groups. These results demonstrate vasoactive properties of acute stroke serum related to stroke subtypes that could potentially contribute to the pathogenesis of early hemodynamic-based clinical events.
authors.affiliation: Department of Neurological Sciences, University of Vermont College of Medicine, 149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA.
authors.forename: Isabella
authors.initials: I
authors.lastname: Canavero
authors.affiliation: Department of Neurological Sciences, University of Vermont College of Medicine, 149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA.
authors.forename: Helene A
authors.initials: HA
authors.lastname: Sherburne
authors.affiliation: Department of Neurological Sciences, University of Vermont College of Medicine, 149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Tremble
authors.affiliation: Department of Neurology, Oregon Stroke Center, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Wayne M
authors.initials: WM
authors.lastname: Clark
authors.affiliation: Department of Neurological Sciences, University of Vermont College of Medicine, 149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA. Marilyn.Cipolla@uvm.edu.|Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Vermont College of Medicine, Burlington, VT, USA. Marilyn.Cipolla@uvm.edu.|Department of Pharmacology, University of Vermont College of Medicine, Burlington, VT, USA. Marilyn.Cipolla@uvm.edu.
authors.forename: Marilyn J
authors.initials: MJ
authors.lastname: Cipolla
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000368:Aged; D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism; D002536:Cerebral Arteries / Q000473:pathology* / Q000503:physiopathology; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D006973:Hypertension / Q000150:complications; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000503:physiopathology*; D009569:Nitric Oxide / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D044967:Serum / Q000378:metabolism*; D013152:Splanchnic Circulation / Q000187:drug effects / Q000502:physiology*; D020521:Stroke / Q000097:blood* / Q000150:complications / Q000473:pathology*; D014527:Uric Acid / Q000097:blood; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D014665:Vasodilator Agents; D009599:Nitroprusside; D014527:Uric Acid; C075750:8-epi-prostaglandin F2alpha; D009569:Nitric Oxide; D002097:C-Reactive Protein; D015237:Dinoprost; D000109:Acetylcholine
keywords: Acute stroke; Circulating factors; Free 8-isoprostane; Inflammation; Oxidative stress; Stroke subtypes
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