title: Quantification of cells expressing markers of proliferation and apoptosis in chronic tonsilitis.
abstract: During chronic tonsillitis, the relationship between proliferation and apoptosis of lymphocytes in tonsillar follicles can be disturbed, which gives rise to attenuation of tonsil immunocompetence and diminishing its contribution in systemic immunity. In this study, we have quantified the cells expressing the markers of proliferation and apoptosis in the follicles of the palatine tonsil. Six tonsils from patients aged 10-29 years with hypertrophic tonsillitis and five tonsils from patients aged 18-22 years with recurrent tonsillitis were studied. The sections of paraffin blocks of tonsillar tissue were stained by the immunohistochemical LSAB/HRP method with the utilisation of antibodies for: Ki-67 antigen-cell marker of proliferation; Bcl-2 and survivin anti-apoptotic factors and Fas/CD95, caspase-3 and Bax pro-apoptotic factors. The size of lymphoid follicles, i.e. mean follicle area and number of lymphoid follicle immunopositive cells per mm2 of a slice area, i.e. numerical areal density were determined by the quantitative image analysis. The localisation of Ki-67, Bcl-2, survivin, Fas/CD95, caspase-3 and Bax- immunopositive cells inside the palatine tonsil was similar in both types of tonsillitis. The number of Ki-67 immunopositive cells was significantly (p < 0.01) larger in the tonsils with hypertrophic tonsillitis (14681.4 ± 1460.5) in comparison to those with recurrent tonsillitis (12491.4 ± 2321.6), although the number of survivin and caspase-3 immunopositive cells was significantly (p < 0.05) larger in recurrent tonsillitis (survivin, 406.9 ± 98.4; caspase-3, 350.4 ± 119.4) when compared to those with hypertrophic tonsillitis (survivin, 117.4 ± 14.5; caspase-3, 210 ± 24). Our results show that the rate of the proliferation and apoptosis of follicular lymphocytes is different in various types of tonsillitis. This suggests that the immunological potential of the palatine tonsil varies in patients with hypertrophic and recurrent tonsillitis, which in practice poses a dilemma over the choice of conservative or surgical treatment.
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine University of Niš, Serbia;
authors.forename: V
authors.initials: V
authors.lastname: Avramović
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine University of Niš, Serbia;
authors.forename: V
authors.initials: V
authors.lastname: Petrović
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine University of Niš, Serbia;
authors.forename: M
authors.initials: M
authors.lastname: Jović
authors.affiliation: Centre for Medical Biochemistry, Clinical Centre Niš, Serbia.
authors.forename: P
authors.initials: P
authors.lastname: Vlahović
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D015415:Biomarkers; D049109:Cell Proliferation; D002648:Child; D005260:Female; D006801:Humans; D006984:Hypertrophy; D007150:Immunohistochemistry; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D014066:Palatine Tonsil; D012008:Recurrence; D014069:Tonsillitis / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D009687:Nuclear Proteins
keywords: Apoptosis; Cell proliferation; Human palatine tonsil; Lymphoid follicle; Quantification
references.citation: Immunity. 2009 Feb 20;30(2):180-92
references.pmid: 19239902
references.citation: Folia Biol (Praha). 2008;54(5):141-5
references.pmid: 19178812
references.citation: Histochem Cell Biol. 2011 Apr;135(4):327-36
references.pmid: 21394653
references.citation: Immunology. 2005 Jan;114(1):2-10
references.pmid: 15606789
references.citation: Clin Cancer Res. 2008 Aug 15;14(16):5000-5
references.pmid: 18698017
references.citation: Clin Exp Immunol. 1978 Mar;31(3):382-90
references.pmid: 350458
references.citation: Clin Otolaryngol Allied Sci. 2003 Jun;28(3):235-9
references.pmid: 12755763
references.citation: Acta Otolaryngol Suppl. 1996;523:68-70
references.pmid: 9082813
references.citation: Int J Pediatr Otorhinolaryngol. 2003 Dec;67 Suppl 1:S69-76
references.pmid: 14662171
references.citation: Pathol Int. 2007 Jul;57(7):391-7
references.pmid: 17587238
references.citation: Clin Exp Immunol. 1980 Feb;39(2):361-70
references.pmid: 6993071
references.citation: Am J Pathol. 2004 Feb;164(2):501-10
references.pmid: 14742256
references.citation: Biochim Biophys Acta. 2011 Jan;1813(1):238-59
references.pmid: 20969895
references.citation: Cancer Res. 2007 Jul 1;67(13):5999-6002
references.pmid: 17616652
references.citation: Eur J Pediatr. 1999 Jun;158(6):469-73
references.pmid: 10378394
references.citation: Anat Embryol (Berl). 2001 Nov;204(5):367-73
references.pmid: 11789984
references.citation: Cytokine. 1995 May;7(4):344-54
references.pmid: 8589265
references.citation: Vet Immunol Immunopathol. 2004 Jun;99(3-4):203-13
references.pmid: 15135986
references.citation: Clin Diagn Lab Immunol. 2004 Nov;11(6):1154-7
references.pmid: 15539521
references.citation: Int J Pediatr Otorhinolaryngol. 2005 Jul;69(7):937-41
references.pmid: 15911012
references.citation: J Immunol. 1993 Jan 1;150(1):39-47
references.pmid: 7678034
references.citation: Mod Pathol. 2003 Apr;16(4):389-94
references.pmid: 12692204
references.citation: Immunology. 2002 Oct;107(2):167-75
references.pmid: 12383195
references.citation: J Pathol. 1999 Oct;189(2):155-60
references.pmid: 10547568
references.citation: J Cell Biol. 1993 Nov;123(3):513-22
references.pmid: 8227122
references.citation: J Immunol. 2003 Aug 15;171(4):1657-66
references.pmid: 12902463
references.citation: Toxicol Pathol. 2007 Jun;35(4):495-516
references.pmid: 17562483
references.citation: Immunol Today. 1998 Sep;19(9):414-21
references.pmid: 9745205
references.citation: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2004 Dec;148(2):225-7
references.pmid: 15744382
references.citation: Biochem J. 2010 Sep 1;430(2):199-205
references.pmid: 20704571
references.citation: Biomark Insights. 2007 Feb 07;1:193-200
references.pmid: 19690649
references.citation: J Pathol. 1997 Sep;183(1):75-9
references.pmid: 9370951