title: One-prime multi-boost strategy immunization with recombinant DNA, adenovirus, and MVA vector vaccines expressing HPV16 L1 induces potent, sustained, and specific immune response in mice.
abstract: Human papillomavirus (HPV) is associated with various human diseases, including cancer, and developing vaccines is a cost-efficient strategy to prevent HPV-related disease. The major capsid protein L1, which an increasing number of studies have confirmed is typically expressed early in infection, is a promising antigen for such a vaccine, although the E6 and E7 proteins have been characterized more extensively. Thus, the L1 gene from HPV16 was inserted into a recombinant vector, AdHu5, and MVA viral vectors, and administered by prime-boost immunization. Virus-like particles were used as control antigens. Our results indicate that prime-boost immunization with heterologous vaccines induced robust and sustained cellular and humoral response specific to HPV16 L1. In particular, sera obtained from mice immunized with DNA + DNA + Ad + MVA had excellent antitumor activity in vivo. However, the data also confirm that virus-like particles can only elicit low levels cellular immunity and not be long-lasting, and are therefore unsuitable for treatment of existing HPV infections.
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, NO.100, YingXin Street, XiCheng District, Beijing 100032, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China.
authors.forename: He-Rong
authors.initials: HR
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China.
authors.forename: Zhi-Yi
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China.
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China. Electronic address: zhouyubai@bjut.edu.cn.
authors.forename: Yu-Bai
authors.initials: YB
authors.lastname: Zhou
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, NO.100, YingXin Street, XiCheng District, Beijing 100032, China. Electronic address: zengy@public.bta.net.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000008:administration & dosage / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; D014614:Vaccines, Synthetic
keywords: Anti-tumor; Cellular immune; HPV; L1; Prime-boost; Therapeutic vaccine