title: In vitro interactions between anidulafungin and nonsteroidal anti-inflammatory drugs on biofilms of Candida spp.
abstract: Candida spp. are responsible for many biomaterial-related infections; they give rise to infective pathologies typically associated with biofilm formation. We recently reported that the echinocandin anidulafungin (ANF) showed a strong in vitro activity against both planktonic and biofilms cells. Herein, we report the antifungal activities of ANF alone and in association with some non-steroidal anti-inflammatory drugs (NSAIDs) against nine Candida strain biofilms: four Candida albicans, two Candida glabrata and three Candida guilliermondii. The activity of ANF was assessed using an in vitro microbiological model relevant for clinical practice. ANF proved oneself to be active against biofilms cells, and a clear-cut synergism was found against Candida species biofilms when ANF was used in combination with three NSAIDs: aspirin, diclofenac, ibuprofen. The positive synergism against Candida spp. of ANF in association with aspirin or the other NSAIDs proved to be a very effective antifungal treatment (FICI<0.5). These results may provide the starting point for new combination therapies of ANF with NSAIDs against Candida biofilm pathologies.
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rosato
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy. Electronic address: alessia.catalano@uniba.it.
authors.forename: Alessia
authors.initials: A
authors.lastname: Catalano
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy. Electronic address: alessia.carocci@uniba.it.
authors.forename: Alessia
authors.initials: A
authors.lastname: Carocci
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Carrieri
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Addolorata
authors.initials: A
authors.lastname: Carone
authors.affiliation: Department of Biomedical Sciences and Human Oncology, Hygiene Section, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Caggiano
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Franchini
authors.affiliation: Department of Pharmacy-Drug Sciences, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Filomena
authors.initials: F
authors.lastname: Corbo
authors.affiliation: Department of Biomedical Sciences and Human Oncology, Hygiene Section, University of Bari, 'Aldo Moro', Bari, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Montagna
mesh_terms: D000077612:Anidulafungin; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D002175:Candida / Q000187:drug effects* / Q000502:physiology; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology; D004357:Drug Synergism; D054714:Echinocandins / Q000494:pharmacology*; D006801:Humans
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000935:Antifungal Agents; D054714:Echinocandins; D000077612:Anidulafungin; D001241:Aspirin
keywords: Anidulafungin; Biofilm; Candida species; Non-steroidal anti-inflammatory drugs (NSAIDs); Synergism; XTT