title: Complex I function in mitochondrial supercomplexes.
abstract: This review discusses the functional properties of mitochondrial Complex I originating from its presence in an assembled form as a supercomplex comprising Complex III and Complex IV in stoichiometric ratios. In particular several lines of evidence are presented favouring the concept that electron transfer from Complex I to Complex III is operated by channelling of electrons through Coenzyme Q molecules bound to the supercomplex, in contrast with the hypothesis that the transfer of reducing equivalents from Complex I to Complex III occurs via random diffusion of the Coenzyme Q molecules in the lipid bilayer. Furthermore, another property provided by the supercomplex assembly is the control of generation of reactive oxygen species by Complex I. This article is part of a Special Issue entitled Respiratory Complex I, edited by Volker Zickermann and Ulrich Brandt.
authors.affiliation: Dipartimento di Scienze Biomediche e Neuromotorie, Alma Mater Studiorum, Università di Bologna, Via Irnerio 48, 40126 Bologna, Italy. Electronic address: giorgio.lenaz@unibo.it.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Lenaz
authors.affiliation: Dipartimento di Scienze Biomediche e Neuromotorie, Alma Mater Studiorum, Università di Bologna, Via Irnerio 48, 40126 Bologna, Italy.
authors.forename: Gaia
authors.initials: G
authors.lastname: Tioli
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
authors.forename: Anna Ida
authors.initials: AI
authors.lastname: Falasca
authors.affiliation: Dipartimento di Scienze Biomediche e Neuromotorie, Alma Mater Studiorum, Università di Bologna, Via Irnerio 48, 40126 Bologna, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Genova
mesh_terms: D000818:Animals; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D004789:Enzyme Activation; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D008956:Models, Chemical; D056004:Molecular Dynamics Simulation; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017494:Proton Pumps / Q000737:chemistry / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000138:chemical synthesis*; D013329:Structure-Activity Relationship; D014451:Ubiquinone / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D046912:Multiprotein Complexes; D017494:Proton Pumps; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D042967:Electron Transport Complex I
keywords: Channelling; Complex I (NADH:ubiquinone oxidoreductase); Mitochondria; ROS; Supercomplex