title: Breast Cancer Stem Cell Isolation.
abstract: Cells within the tumor are highly heterogeneous. Only a small portion of the cells within the tumor is capable to generate a new tumor. These cells are called cancer stem cells. Theoretically, cancer stem cells are originally from normal stem cells or early progenitor cells which accumulate the random mutations and undergo an altered version of the normal differentiation process. The cancer stem cell drives tumor progression and its recurrence. Thus, the technique to identify and purify the cancer stem cell is the key in any cancer stem cell research. In this protocol, we provide the basic technology of identification and purification of breast cancer stem cells as well as further functional assays to help the researchers achieve their research goals.
authors.affiliation: Department of Cancer Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA, 19107, USA.
authors.forename: Xuanmao
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Kazan Federal University, 18 Kremlyovskaya St, Kazan, Republic of Tatarstan, 420008, Russia.
authors.forename: Albert A
authors.initials: AA
authors.lastname: Rizvanov
authors.affiliation: Department of Cancer Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA, 19107, USA.
authors.forename: Massimo
authors.initials: M
authors.lastname: Cristofanilli
authors.affiliation: Kazan Federal University, 18 Kremlyovskaya St, Kazan, Republic of Tatarstan, 420008, Russia.
authors.forename: Regina R
authors.initials: RR
authors.lastname: Miftakhova
authors.affiliation: Department of Cancer Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA, 19107, USA. richard.pestell@jefferson.edu.|Kazan Federal University, 18 Kremlyovskaya St, Kazan, Republic of Tatarstan, 420008, Russia. richard.pestell@jefferson.edu.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Pestell
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D051927:CD24 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D051379:Mice; D014411:Neoplastic Stem Cells / Q000473:pathology*
chemical_list: D051927:CD24 Antigen; D018960:Hyaluronan Receptors
keywords: Breast cancer stem cells; Circulating tumor cells; Fluorescence-activated cell sorting (FACS); Mammosphere formation; Transplantation assays