title: Increases of microRNA let-7e in peripheral blood mononuclear cells in Hashimoto's disease.
abstract: MicroRNA (miRNA) is a family of non-coding RNAs that have important roles in various vital functions. It has been reported that let-7e, a miRNA, may be involved in the regulation of interleukin (IL)-10 production. The purpose of this study was to evaluate the role of let-7e as a regulator of IL-10 production in the pathological processes of autoimmune thyroid diseases (AITDs). We evaluated the association between let-7e expression and intracellular expression of IL-10 in the peripheral blood mononuclear cells (PBMCs) collected from 11 healthy volunteers. Then we investigated the expression levels of let-7e in the PBMCs of 50 patients with Graves' disease (GD), 42 patients with Hashimoto's disease (HD) and 28 healthy controls. We found negative correlations between the expression level of let-7e and IL-10 messengerRNA (mRNA) and between the expression level of let-7e and proportion of IL-10(+) cells in stimulated PBMCs from healthy volunteers (r = -0.44, p = 0.0267 and r = -0.49, p = 0.0166, respectively). The expression levels of let-7e were significantly increased in HD patients compared with those in GD patients and healthy volunteers (p = 0.0003 and p = 0.0011, respectively). let-7e may be associated with the pathogenesis of HD through the regulation of intracellular IL-10 expression.
authors.affiliation: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kagawa
authors.forename: Mikio
authors.initials: M
authors.lastname: Watanabe
authors.forename: Naoya
authors.initials: N
authors.lastname: Inoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Otsu
authors.forename: Minori
authors.initials: M
authors.lastname: Saeki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Katsumata
authors.forename: Yukina
authors.initials: Y
authors.lastname: Takuse
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwatani
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D050031:Hashimoto Disease / Q000097:blood / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D015854:Up-Regulation / Q000235:genetics; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; D016753:Interleukin-10