title: [Study on virulence factors of Candida tropicalis isolated from clinical samples].
abstract: OBJECTIVE
  To determine the in vitro production of virulence factors for Candida (C.) tropicalis, including aspartyl proteinases, phospholipases and hemolytic activities, describe the regulation of virulence factors varying with time in C. tropicalis, and analyze the differences in aspartyl proteinases and hemolytic activities of C. tropicalis isolated from anatomically distinct sites.
  
  
  METHODS
  A total of 64 C. tropicalis strains were spot-inoculated onto bovine albumin agar, egg yolk agar and sheep blood agar plates, respectively. Then the plates were incubated for 24, 48 and 72 hour at 37 °C, respectively. The aspartyl proteinases, phospholipase and hemolytic activities were determined at each time point, respectively.
  
  
  RESULTS
  All the C. tropiclais isolates showed positive aspartyl proteinases and hemolytic activities at each time point, but no phospholipases activity was detected in C. tropicalis. On comparison of aspartyl proteinases and hemolytic activities at different time points, aspartyl proteinases activity at 48 and 72 hour was higher than that at 24 hour. During 72 hour, hemolytic activity of C. tropicalis increased. No statistical significant differences in aspartyl proteinases and hemolytic activities of C. tropicalis were observed among different infection sites (P=0.368 and 0.985).
  
  
  CONCLUSIONS
  The C. tropicalis clinical isolates in China have aspartyl proteinases activity, hemolytic activity, but have no phospholipase activity.
authors.affiliation: State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Shuanbao
authors.initials: S
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Wenge
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Che
authors.affiliation: State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Funing
authors.initials: F
authors.lastname: Bian
authors.affiliation: State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Email: wuyuan@icdc.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D057055:Aspartic Acid Proteases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D041022:Candida tropicalis / Q000378:metabolism* / Q000472:pathogenicity; D002417:Cattle; D002681:China; D003470:Culture Media; D006801:Humans; D010740:Phospholipases / Q000378:metabolism; D012756:Sheep; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D037521:Virulence Factors; D010740:Phospholipases; D057055:Aspartic Acid Proteases