title: IGF-I induces adipose derived mesenchymal cell chondrogenic differentiation in vitro and enhances chondrogenesis in vivo.
abstract: Recent studies have demonstrated that insulin-like growth factor-1 (IGF-I) modulates bone mesenchymal stem cell chondrogenic differentiation independent of transforming growth factor beta (TGF-β) signaling in vitro. However, it is unclear whether IGF-I can solely modulate human adipose-derived mesenchymal cell (hAMC) chondrogenic differentiation, or whether it has additive effects with TGF-β1 to induce chondrogenic differentiation in vitro and development of mature cartilage in vivo. We investigated the effect of IGF-I on the induction of hAMC chondrogenic differentiation in the presence or absence of transforming growth factor beta 1 (TGF-β1) in vitro, and chondrogenesis of the induced hAMC in vivo. The results showed that IGF-I alone induced collagen type II, aggrecan, and Sox9 mRNA expression and collagen type II and aggrecan proteins expressions in hAMCs. Notably, there was greater mRNA expression of collagen type II, aggrecan and Sox9, and greater protein expression of collagen type II and aggrecan following TGF-β1 + IGF-I treatment, compared to either TGF-β1 or IGF-I-treated hAMCs. These results were confirmed in cartilage tissues derived from induced hAMCs. These findings indicate that IGF-I alone has the ability to induce chondrogenic differentiation and has additive effects with TGF-β1 to induce chondrogenic differentiation in vitro and in vivo.
authors.affiliation: Department of Orthopaedics, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second Hospital, No. 62 Huaihai Road South, Huai'an, 223002, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Joint Surgery, Second People's Hospital of Hunan Province, Changsha, 410007, China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second Hospital, No. 62 Huaihai Road South, Huai'an, 223002, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second Hospital, No. 62 Huaihai Road South, Huai'an, 223002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
authors.affiliation: Department of Orthopaedics, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second Hospital, No. 62 Huaihai Road South, Huai'an, 223002, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second Hospital, No. 62 Huaihai Road South, Huai'an, 223002, China. wuque1@hotmail.com.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology; D000293:Adolescent; D000328:Adult; D053674:Aggrecans / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis / Q000187:drug effects*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000096:biosynthesis; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects; D055815:Young Adult
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; C583557:IGF1 protein, human; D012333:RNA, Messenger; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D053773:Transforming Growth Factor beta1; D007334:Insulin-Like Growth Factor I
keywords: Chondrogenic differentiation; Insulin-like growth factor; Multipotentmesenchymal cells; Tissue engineering
references.citation: Ann Rheum Dis. 1992 Apr;51(4):440-7
references.pmid: 1586240
references.citation: J Rheumatol. 1990 Sep;17(9):1217-21
references.pmid: 2290165
references.citation: Mol Biol Cell. 2002 Dec;13(12):4279-95
references.pmid: 12475952
references.citation: Biochem Biophys Res Commun. 2005 Apr 29;330(1):142-50
references.pmid: 15781243
references.citation: Toxicol Appl Pharmacol. 2013 May 15;269(1):25-33
references.pmid: 23454400
references.citation: J R Soc Interface. 2013 Dec 18;11(92 ):20130784
references.pmid: 24352667
references.citation: J Bone Miner Res. 2006 Apr;21(4):626-36
references.pmid: 16598383
references.citation: Br J Rheumatol. 1993 Apr;32(4):274-80
references.pmid: 8461919
references.citation: Tissue Eng Part A. 2014 Apr;20(7-8):1271-84
references.pmid: 24251600
references.citation: Inflamm Res. 1998 Mar;47(3):90-100
references.pmid: 9562333
references.citation: J Cell Biochem. 2003 Aug 15;89(6):1235-49
references.pmid: 12898521
references.citation: Cells Tissues Organs. 2003;174(3):101-9
references.pmid: 12835573
references.citation: J Biol Chem. 2003 Sep 19;278(38):36563-71
references.pmid: 12853454
references.citation: Nature. 2003 Oct 9;425(6958):577-84
references.pmid: 14534577
references.citation: Tissue Eng. 2002 Feb;8(1):73-84
references.pmid: 11886656
references.citation: Exp Neurol. 2007 Oct;207(2):267-74
references.pmid: 17761164
references.citation: Osteoarthritis Cartilage. 2003 Jan;11(1):55-64
references.pmid: 12505488
references.citation: J Orthop Res. 2009 May;27(5):612-9
references.pmid: 18985688
references.citation: Biochem Biophys Res Commun. 2006 May 5;343(2):644-52
references.pmid: 16554022
references.citation: J Cell Mol Med. 2005 Oct-Dec;9(4):929-39
references.pmid: 16364200
references.citation: J Biomech. 2014 Jun 27;47(9):2183-8
references.pmid: 24210471
references.citation: J Biosci Bioeng. 2007 Jan;103(1):98-100
references.pmid: 17298908
references.citation: J Tissue Eng Regen Med. 2007 May-Jun;1(3):170-8
references.pmid: 18038408
references.citation: J Cell Physiol. 2007 Jun;211(3):682-91
references.pmid: 17238135
references.citation: J Orthop Res. 2001 Jul;19(4):738-49
references.pmid: 11518286
references.citation: Hum Gene Ther. 2002 Sep 1;13(13):1621-30
references.pmid: 12228017
references.citation: J Cell Physiol. 2012 May;227(5):2003-12
references.pmid: 21751209
references.citation: Cells Tissues Organs. 2014;199(2-3):117-30
references.pmid: 25413333
references.citation: J Clin Endocrinol Metab. 1996 Mar;81(3):1096-103
references.pmid: 8772582
references.citation: Int J Stem Cells. 2014 Nov;7(2):143-52
references.pmid: 25473452
references.citation: J Cell Biochem. 1998 Dec 1;71(3):382-91
references.pmid: 9831075
references.citation: J Cell Biol. 1991 Nov;115(4):1091-105
references.pmid: 1955457
references.citation: Cell Transplant. 2011;20(10):1575-88
references.pmid: 21294960
references.citation: Int Orthop. 2013 May;37(5):937-43
references.pmid: 23322062
references.citation: Cells Tissues Organs. 2006;183(2):55-61
references.pmid: 17053321
references.citation: J Orthop Res. 1998 Sep;16(5):531-41
references.pmid: 9820275
references.citation: Biochem Biophys Res Commun. 2004 Jul 30;320(3):914-9
references.pmid: 15240135
references.citation: Eur Cell Mater. 2013 Apr 16;25:229-47
references.pmid: 23588785
references.citation: PLoS One. 2013 Dec 31;8(12):e84206
references.pmid: 24391913
references.citation: J Bone Joint Surg Br. 2002 Mar;84(2):276-88
references.pmid: 11922373