title: Loss of phospholipase D2 impairs VEGF-induced angiogenesis.
abstract: Vascular endothelial growth factor (VEGF) is a key mediator of angiogenesis and critical for normal embryonic development and repair of pathophysiological conditions in adults. Although phospholipase D (PLD) activity has been implicated in angiogenic processes, its role in VEGF signaling during angiogenesis in mammals is unclear. Here, we found that silencing of PLD2 by siRNA blocked VEGF-mediated signaling in immortalized human umbilical vein endothelial cells (iHUVECs). Also, VEGF-induced endothelial cell survival, proliferation, migration, and tube formation were inhibited by PLD2 silencing. Furthermore, while Pld2-knockout mice exhibited normal development, loss of PLD2 inhibited VEGF-mediated ex vivo angiogenesis. These findings suggest that PLD2 functions as a key mediator in the VEGF-mediated angiogenic functions of endothelial cells. [BMB Reports 2016; 49(3): 191-196].
authors.forename: Chang Sup
authors.initials: CS
authors.lastname: Lee
authors.forename: Jaewang
authors.initials: J
authors.lastname: Ghim
authors.forename: Parkyong
authors.initials: P
authors.lastname: Song
authors.forename: Pann-Ghill
authors.initials: PG
authors.lastname: Suh
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D002461:Cell Line, Transformed; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D018345:Mice, Knockout; D018919:Neovascularization, Physiologic; D010739:Phospholipase D / Q000172:deficiency / Q000378:metabolism*; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D042461:Vascular Endothelial Growth Factor A; C105606:phospholipase D2; D010739:Phospholipase D
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