title: Frontally eluted components procedure with thin layer chromatography as a mode of sample preparation for high performance liquid chromatography quantitation of acetaminophen in biological matrix.
abstract: A new concept of using thin-layer chromatography to sample preparation for the quantitative determination of solute/s followed by instrumental techniques is presented Thin-layer chromatography (TLC) is used to completely separate acetaminophen and its internal standard from other components (matrix) and to form a single spot/zone containing them at the solvent front position (after the final stage of the thin-layer chromatogram development). The location of the analytes and internal standard in the solvent front zone allows their easy extraction followed by quantitation by HPLC. The exctraction procedure of the solute/s and internal standard can proceed from whole solute frontal zone or its part without lowering in accuracy of quantitative analysis.
authors.affiliation: Medical University of Lublin, Department of Physical Chemistry, Chodźki 4a Str., 20-098 Lublin, Poland. Electronic address: anna.klimek@umlub.pl.
authors.forename: A
authors.initials: A
authors.lastname: Klimek-Turek
authors.affiliation: Medical University of Lublin, Department of Physical Chemistry, Chodźki 4a Str., 20-098 Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Sikora
authors.affiliation: Medical University of Lublin, Department of Physical Chemistry, Chodźki 4a Str., 20-098 Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Rybicki
authors.affiliation: Medical University of Lublin, Department of Physical Chemistry, Chodźki 4a Str., 20-098 Lublin, Poland.
authors.forename: T H
authors.initials: TH
authors.lastname: Dzido
mesh_terms: D000082:Acetaminophen / Q000032:analysis* / Q000097:blood; D000083:Acetanilides / Q000032:analysis / Q000097:blood; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D012997:Solvents
chemical_list: D000083:Acetanilides; D012997:Solvents; D000082:Acetaminophen; C508827:acetanilide
keywords: Frontally eluted components; Matrix effects; Sample preparation