title: EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma.
abstract: EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial-mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Isadora L
authors.initials: IL
authors.lastname: Flores
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Kawahara
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil Departamento de Odontologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Márcia C C
authors.initials: MC
authors.lastname: Miguel
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Daniela C
authors.initials: DC
authors.lastname: Granato
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Romênia R
authors.initials: RR
authors.lastname: Domingues
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Carolina C S
authors.initials: CC
authors.lastname: Macedo
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Carolina M
authors.initials: CM
authors.lastname: Carnielli
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Sami
authors.initials: S
authors.lastname: Yokoo
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Priscila C
authors.initials: PC
authors.lastname: Rodrigues
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil Departamento de Odontologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Bárbara V B
authors.initials: BV
authors.lastname: Monteiro
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Carine E
authors.initials: CE
authors.lastname: Oliveira
authors.affiliation: Departamento de Prótese e Periodontia, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Salmon
authors.affiliation: Departamento de Prótese e Periodontia, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Francisco H
authors.initials: FH
authors.lastname: Nociti
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Márcio A
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Alan
authors.initials: A
authors.lastname: Santos-Silva
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Flavia V
authors.initials: FV
authors.lastname: Winck
authors.affiliation: Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil coletta@fop.unicamp.br adriana.paesleme@lnbio.cnpem.br.
authors.forename: Ricardo D
authors.initials: RD
authors.lastname: Coletta
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil coletta@fop.unicamp.br adriana.paesleme@lnbio.cnpem.br.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010641:Phenotype; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: C000591363:EEF1D protein, human; D020648:Peptide Elongation Factor 1
keywords: epithelial–mesenchymal transition; eukaryotic translation elongation factor 1δ; oral cancer