title: The outer membrane porin OmpW of Acinetobacter baumannii is involved in iron uptake and colistin binding.
abstract: This study was undertaken to characterize functions of the outer membrane protein OmpW, which potentially contributes to the development of colistin- and imipenem-resistance in Acinetobacter baumannii. Reconstitution of OmpW in artificial lipid bilayers showed that it forms small channels (23 pS in 1 m KCl) and markedly interacts with iron and colistin, but not with imipenem. In vivo, (55) Fe uptake assays comparing the behaviours of ΔompW mutant and wild-type strains confirmed a role for OmpW in A. baumannii iron homeostasis. However, the loss of OmpW expression did not have an impact on A. baumannii susceptibilities to colistin or imipenem.
authors.affiliation: CNRS UMR 6270 & FR3038, Normandie Univ, Laboratoire Polymères, Biopolymères & Surfaces, Université de Rouen, Mont Saint Aignan, France.
authors.forename: Manuella
authors.initials: M
authors.lastname: Catel-Ferreira
authors.affiliation: CNRS UMR 6270 & FR3038, Normandie Univ, Laboratoire Polymères, Biopolymères & Surfaces, Université de Rouen, Mont Saint Aignan, France.|Center for International Health Research, CRESIB, Hospital Clínic, University of Barcelona, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Marti
authors.affiliation: UMR 7242, Université de Strasbourg-CNRS, ESBS, Illkirch, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Guillon
authors.affiliation: Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Jara
authors.affiliation: COBRA-CNRS, Laboratoire de RMN et Modélisation moléculaire, UMR 6014 & FR3038 CNRS, Université de Rouen, Mont Saint Aignan, France.
authors.forename: Gaël
authors.initials: G
authors.lastname: Coadou
authors.affiliation: Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Universités de Montpellier II et I, CNRS; UMR 5235, Montpellier Cedex 05, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Molle
authors.affiliation: Laboratoire de Microbiologie Signaux et Micro-Environnement (LMSM) EA 4312, Université de Rouen, Evreux, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Bouffartigues
authors.affiliation: Servicio de Microbiología-INIBIC, Complejo Hospitalario Universitario A Coruña (CHUAC), Spain.
authors.forename: German
authors.initials: G
authors.lastname: Bou
authors.affiliation: UMR 7242, Université de Strasbourg-CNRS, ESBS, Illkirch, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Shalk
authors.affiliation: CNRS UMR 6270 & FR3038, Normandie Univ, Laboratoire Polymères, Biopolymères & Surfaces, Université de Rouen, Mont Saint Aignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: Center for International Health Research, CRESIB, Hospital Clínic, University of Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Vila-Farrés
authors.affiliation: CNRS UMR 6270 & FR3038, Normandie Univ, Laboratoire Polymères, Biopolymères & Surfaces, Université de Rouen, Mont Saint Aignan, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Dé
mesh_terms: D040981:Acinetobacter baumannii / Q000378:metabolism*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003091:Colistin / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D018272:Porins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D011994:Recombinant Proteins; D007501:Iron; D003091:Colistin
keywords: colistin resistance; hydrophobic channel; iron homeostasis