title: Aberrant promoter methylation of SH3GL2 gene in vulvar squamous cell carcinoma correlates with clinicopathological characteristics and HPV infection status.
abstract: OBJECTIVE
  This study attempted to examine the methylation status of SH3GL2 gene in different types of human vulvar lesions and its correlation with clinicopathological parameters.
  
  
  METHODS
  Immunohistochemical analysis was used to identify the expression status of SH3GL2 in vulvar squamous cell carcinoma (VSCC), vulvar intraepithelial neoplasia (VIN) and benign vulvar squamous epithelium tissues. Bisulfite genomic sequencing method was used to detect methylation status of the SH3GL2 gene. Clinicopathological correlation of the alterations was analysed by the chi-square tests.
  
  
  RESULTS
  Immunohistochemical analysis showed expression of SH3GL2 in VSCC was significantly downregulated than that in VIN and normal vulvar tissues. In accordance with higher frequency of methylation status in SH3GL2, statistical analysis showed methylation status of SH3GL2 was closely related to tumor TNM stage (P=0.003), but not related to age, tumor volume, tumor differentiation, lymph node metastasis and VIN grade. High-methylation status of SH3GL2 showed significant association with HPV infection status.
  
  
  CONCLUSIONS
  Our results indicated that the methylation status of SH3GL2 gene was associated with the TNM staging and HPV infection status of VSCC, suggesting that it might play a synergistic role in the development of VSCC.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Shitai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ouyang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002278:Carcinoma in Situ / Q000737:chemistry / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics* / Q000473:pathology / Q000821:virology; D019175:DNA Methylation; D018450:Disease Progression; D015536:Down-Regulation; D004847:Epithelial Cells / Q000737:chemistry / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology; D011401:Promoter Regions, Genetic; D014846:Vulvar Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C107640:SH3GL2 protein, human
keywords: SH3GL2; methylation; vulvar squamous cell carcinoma
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