title: Periodic peristalsis releasing constrained water in high solids enzymatic hydrolysis of steam exploded corn stover.
abstract: Periodic peristalsis was used to release water constraint and increase high solids enzymatic hydrolysis efficiency. Glucan and xylan conversion in periodic peristalsis enzymatic hydrolysis (PPEH) at 21% solid loading increased by 5.2-6.4% and 6.8-8.8% compared with that in incubator shaker enzymatic hydrolysis (ISEH), respectively. Hydrolysis kinetics suggested that sugars conversion significantly increased within 24h in PPEH compared with ISEH. The peak height of main water pool increased by 7.7-43.1% within 24h in PPEH compared with ISEH. The increases in peak height of main water pool were consistent with the increases in glucan conversion. Submicroscopic particulates and macro granule residues contributed greatly to water constraint compared with glucose, xylose, ethanol, and Tween 80. Smaller particle size and longer residence time resulted in lower water constraint and facilitated the enzymatic hydrolysis performance. Periodic peristalsis was an effective method to reduce water constraint and increase high solids enzymatic hydrolysis efficiency.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Graduate University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: hzchen@ipe.ac.cn.
authors.forename: Hong-Zhang
authors.initials: HZ
authors.lastname: Chen
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D013227:Steam; D014867:Water / Q000737:chemistry; D014994:Xylose / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D005936:Glucans; D013227:Steam; D014867:Water; D000431:Ethanol; D014994:Xylose; D005947:Glucose
keywords: High solids enzymatic hydrolysis; Particle size; Periodic peristalsis; Steam explosion; Water constraint