title: Nesterenkonia aurantiacasp. nov., an alkaliphilic actinobacterium isolated from Antarctica.
abstract: A Gram-stain-positive, non-endospore-forming, haloalkaliphilic actinobacterium, strain CK5T, was isolated from a soil sample, collected at Cape King (Antarctica), and its taxonomic position was investigated by using a polyphasic approach. Cells were cocci with orange pigmentation, non-motile and grew optimally at 25 °C and pH 9.0-9.5 in the presence of 2 % (w/v) NaCl. Cellular membrane contained MK-7 (72 %) and MK-8 (28 %), and anteiso-C15 : 0(64.8 %), iso-C16 : 0(13.3 %),n-C17 : 0(9.9 %),n-C16 : 0(4.0 %),n-C14 : 0(3.7 %) as major cellular fatty acids. The DNA G+C content was 64.8 mol%. Strain CK5T, based on the 16S rRNA gene sequence similarity, was most closely related toNesterenkonia jeotgaliJG-241T(99.5 %),Nesterenkonia sandarakinaYIM 70009T(99.4 %),Nesterenkonia luteaYIM 70081T(99.4 %),Nesterenkonia halotoleransYIM 70084T(99.3 %),Nesterenkonia xinjiangensisYIM 70097T(97.2 %),Nesterenkonia flavaCAAS 251T(97.1 %) andNesterekonia aethiopicaCCUG 48939T(97.1 %). Strain CK5Trevealed 31 % DNA-DNA relatedness with respect toN. sandarakinaDSM 15664T, 29 % with respect toN. jeotgaliDSM 19081T, 10 % with respect toN. luteaDSM 15666Tand 1 % with respect toN. halotolerans,DSM 15474T,N. xinjiangensisDSM 15475T,N. aethiopicaDSM 17733TandN. flavaDSM 19422T. On the basis of 16S rRNA gene sequences, DNA-DNA hybridization and chemotaxonomic characteristics, strain CK5Trepresents a novel species of the genusNesterenkonia, for which the nameNesterenkonia aurantiacasp. nov. is proposed. The type strain is CK5T( = DSM 27373T= JCM 19723T).
authors.affiliation: Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare (I.C.B.), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Finore
authors.affiliation: Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Biochimica delle Proteine (I.B.P.), Via P. Castellino 111, 80124, Naples, Italy.
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Orlando
authors.affiliation: Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare (I.C.B.), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.|University of Naples 'Parthenope', Department of Sciences and Technologies, Centro Direzionale, Naples, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Di Donato
authors.affiliation: Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare (I.C.B.), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Leone
authors.affiliation: Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare (I.C.B.), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicolaus
authors.affiliation: Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare (I.C.B.), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.
authors.forename: Annarita
authors.initials: A
authors.lastname: Poli