title: MWCNT-Fe3O4-based immuno-PCR for the early screening of nasopharyngeal carcinoma.
abstract: Nasopharyngeal carcinoma (NPC) is the most prevalent form of malignancy in southeast China and its development is meticulously related to EBV pathogenesis. The current screening techniques are unsatisfactory in terms of the sensitivity and hence most of the NPC patients are diagnosed at an advanced stage. Herein, we report the multi-walled carbon nanotubes (MWCNTs) combined with iron oxide nanoparticles as a sensing surface for the early screening of nasopharyngeal carcinoma (NPC) by immuno-PCR (iPCR). The MWCNT-Fe3O4 nanocomposite was characterized by Fourier transform infrared spectra (FTIR), Raman spectra, X-ray diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). The characterization techniques had confirmed the successful formation of MWCNT-Fe3O4 nanocomposites. The MWCNT-Fe3O4-based iPCR was effectively tested for the quantification of anti-EBV antibodies in human serum and the limit of detection (LOD) was compared with ELISA. The limit of detection by iPCR was valid until 1:10,000,000 fold dilution of NPC(+ve) human serum, whereas ELISA can detect the anti-EBV antibodies in human serum up to 1:100,000 fold dilution. The MWCNT-Fe3O4 offers an excellent surface area for the antigen-antibody binding and hence greater sensitivity was achieved.
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Liu
authors.initials: L
authors.lastname: Chia-Ching
authors.affiliation: Department of Microbial Biotechnology, Bharathiar University, Coimbatore 641 046, India.
authors.forename: Sadhasivam
authors.initials: S
authors.lastname: Subramaniam
authors.affiliation: Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chennai, India.
authors.forename: Savitha
authors.initials: S
authors.lastname: Sivasubramanian
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan; Division of Biomedical Engineering and Nanomedicine Research, National Health Research Institutes, Miaoli 350, Taiwan. Electronic address: double@ntu.edu.tw.
authors.forename: Lin
authors.initials: L
authors.lastname: Feng-Huei
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D002277:Carcinoma; D004797:Enzyme-Linked Immunosorbent Assay; D019309:Epstein-Barr Virus Nuclear Antigens / Q000737:chemistry / Q000276:immunology; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000914:Antibodies, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007070:Immunoglobulin A; D037742:Nanotubes, Carbon; C417029:EBV-encoded nuclear antigen 1; D052203:Ferrosoferric Oxide
keywords: EBNA1 antigen; MWCNT; NPC; Nanocomposites; iPCR