title: Methyl viologen induces neural differentiation on murine P19 cells.
abstract: Methyl viologen is a highly effective contact herbicide, a neurotoxic compound and an inducer of reactive oxygen species, which generate oxidative stress in cells. Reactive oxygen species has been known to function as an important messenger in cell differentiation. In this study, we investigated the effect of methyl viologen on neural cell differentiation using pluripotent mouse embryonal carcinoma P19 cells, which reportedly differentiate into neural cells upon exposure to retinoic acid. Methyl viologen, an inducer of intracellular reactive oxygen species, induced the differentiation of P19 cells into neural cells in the presence of neurofilament. Reduced glutathione, an eliminator of reactive oxygen species, also induced neural differentiation in P19 cells. These results suggest that P19 cells differentiate into neural cells, conceivably independent of intracellular reactive oxygen species.
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa, 237-0061, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Shimada
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa, 237-0061, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa, 237-0061, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Tsutsui
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa, 237-0061, Japan. tsuruwaka@jamstec.go.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsuruwaka
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D007382:Intermediate Filaments / Q000187:drug effects / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology*; D010269:Paraquat / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D014212:Tretinoin; D048051:p38 Mitogen-Activated Protein Kinases; D005978:Glutathione; D010269:Paraquat
keywords: Differentiation; Methyl viologen; Oxidative stress; P19; ROS
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