title: Identification of a conserved 5'-dRP lyase activity in bacterial DNA repair ligase D and its potential role in base excision repair.
abstract: Bacillus subtilis is one of the bacterial members provided with a nonhomologous end joining (NHEJ) system constituted by the DNA-binding Ku homodimer that recruits the ATP-dependent DNA Ligase D (BsuLigD) to the double-stranded DNA breaks (DSBs) ends. BsuLigD has inherent polymerization and ligase activities that allow it to fill the short gaps that can arise after realignment of the broken ends and to seal the resulting nicks, contributing to genome stability during the stationary phase and germination of spores. Here we show that BsuLigD also has an intrinsic 5'-2-deoxyribose-5-phosphate (dRP) lyase activity located at the N-terminal ligase domain that in coordination with the polymerization and ligase activities allows efficient repairing of 2'-deoxyuridine-containing DNA in an in vitro reconstituted Base Excision Repair (BER) reaction. The requirement of a polymerization, a dRP removal and a final sealing step in BER, together with the joint participation of BsuLigD with the spore specific AP endonuclease in conferring spore resistance to ultrahigh vacuum desiccation suggest that BsuLigD could actively participate in this pathway. We demonstrate the presence of the dRP lyase activity also in the homolog protein from the distantly related bacterium Pseudomonas aeruginosa, allowing us to expand our results to other bacterial LigDs.
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa' (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Nicolás Cabrera 1, 28049 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: de Ory
authors.affiliation: Radiation Biology Department, German Aerospace Center (DLR), Institute of Aerospace Medicine, Linder Hoehe, D-51147 Cologne, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Nagler
authors.affiliation: Centro Nacional de Biotecnología (Consejo Superior de Investigaciones Científicas), Darwin 3, 28049 Madrid, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Carrasco
authors.affiliation: Radiation Biology Department, German Aerospace Center (DLR), Institute of Aerospace Medicine, Linder Hoehe, D-51147 Cologne, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Raguse
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa' (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Nicolás Cabrera 1, 28049 Madrid, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Zafra
authors.affiliation: Radiation Biology Department, German Aerospace Center (DLR), Institute of Aerospace Medicine, Linder Hoehe, D-51147 Cologne, Germany mdevega@cbm.csic.es.
authors.forename: Ralf
authors.initials: R
authors.lastname: Moeller
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa' (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Nicolás Cabrera 1, 28049 Madrid, Spain mdevega@cbm.csic.es.
authors.forename: Miguel
authors.identifier: http://orcid.org/0000-0003-1285-7549
authors.initials: M
authors.lastname: de Vega
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair / Q000235:genetics*; D011088:DNA Ligases / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology
chemical_list: C423906:5'-deoxyribose phosphate lyase; D019761:Phosphorus-Oxygen Lyases; D011088:DNA Ligases
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