title: [Mediated mechanism of NDRG2 in the regulation of neutrophil nuclear transcription factor-κB signaling pathway through the deubiquitylating enzyme of Zc3h12d].
abstract: OBJECTIVE
  To explore the role NDRG2/Zc3h12d in the regulation of neutrophil nuclear transcription factor-κB signaling pathway and the underlying mechanisms.
  
  
  METHODS
  Sixteen HBE cells were incubated with lipopolysaccharide (LPS) to establish airway mucus hypersecretion model, which was transfected with NDRG2 or Zc3h12d siRNA. The cells were divided into 5 groups: a LPS+NDRG2 siRNA (Group A), a LPS+ NDRG2 and Zc3h12d siRNAs (Group B), a LPS+Zc3h12d siRNA (Group C), a LPS+ empty plasmid (Group D), and a negative control group (Group E). The reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the expression level of mucin (MUC) 5AC mRNA. The levels of MUC5AC and the inflammatory factors were examined by the enzyme-linked immunosorbent assay (ELISA). The NF-κB p65 and the Zc3h12d protein levels were measured by Western blot. The MUC5C expression was further examined by laser confocal method.
  
  
  RESULTS
  Compared with Group B, the levels of MUC5AC mRNA and protein in Group A were decreased (P<0.05), there was no significant difference in the MUC5AC mRNA and MUC5AC protein levels between the Group B and the Group C (P>0.05). Compared with Group D, the MUC5AC (mRNA and protein) and inflammatory factor levels in the Group A were significantly decreased (P<0.05); compared with Group E, after incubation with LPS, the levels of MUC5AC (mRNA and protein) and inflammatory factors in the Group D was increased, with significantly difference (P<0.05).
  
  
  CONCLUSIONS
  NDRG2 can regulate the function of NF-κB signaling pathway through the deubiquitylating enzyme of Zc3h12d.
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010; Department of Respiratory Meddicine, Affiliated Hospital of Hainan Medical College, Haikou 570100, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Respiratory Meddicine, Affiliated Hospital of Hainan Medical College, Haikou 570100, China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Respiratory Meddicine, Affiliated Hospital of Hainan Medical College, Haikou 570100, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
mesh_terms: D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D004720:Endonucleases; D004722:Endoribonucleases; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides; D055271:Mucin 5AC / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D012137:Respiratory System / Q000503:physiopathology; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism*; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D064570:Ubiquitin-Specific Proteases / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D008070:Lipopolysaccharides; C524678:MUC5AC protein, human; D055271:Mucin 5AC; C466228:NDRG2 protein, human; D011506:Proteins; C498143:RELA protein, human; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA; D025521:Tumor Suppressor Proteins; D004720:Endonucleases; D004722:Endoribonucleases; C489862:ZC3H12D protein, human; D064570:Ubiquitin-Specific Proteases