title: Structural and functional evaluation of oxygenating keratin/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering.
abstract: In this study, we report a new type of oxygen-generating scaffold, composed of human keratin, silk, gelatin and calcium peroxide (CPO). After mixing the silk/keratin (60:40) with 2% gelatin and 20% CPO, the film demonstrated excellent mechanical properties, non-cytotoxicity and oxygen-generative ability. The detailed structure of scaffold was revealed by confocal laser and electronic scanning microscopy. The gelatin formed the network structure, which mixed with silk fibroin and keratin. The CPOs were embedded into scaffold. A shell-core structure was formed in the CPO particles, in which the CPO was located in the core and the gelatin was mainly wrapped around the CPO. Furthermore, the oxygen-release test showed that scaffold was able to steadily release high level of oxygen over two weeks in vitro. In addition, the anti-bacterial function was also proved in the scaffold. Films with CPO enhanced the repair in dog urethral defect models, resulting in patent urethra. Improved organized muscle bundles and epithelial layer were observed in animals treated with CPO films compared with those treated with non-CPO films. This study suggests that this biomaterial could be suitable for tissue engineered urinary tract reconstruction.
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: XiangGuo
authors.initials: X
authors.lastname: Lv
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, China.
authors.forename: ShiYan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: MinKai
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: JianWen
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: XuFeng
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: RanXing
authors.initials: R
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, China.
authors.forename: HuaPing
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China; Shanghai Eastern Urological Reconstruction and Repair Institute, Shanghai, China.
authors.forename: YueMin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China. Electronic address: fengchaomed@sina.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology* / Q000648:ultrastructure; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D007633:Keratins / Q000737:chemistry* / Q000494:pharmacology* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D010545:Peroxides / Q000494:pharmacology; D011817:Rabbits; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014521:Urethra / Q000187:drug effects / Q000502:physiology*; D055114:X-Ray Microtomography
chemical_list: D000900:Anti-Bacterial Agents; D010545:Peroxides; D007633:Keratins; C403632:calcium peroxide; D005780:Gelatin; D005349:Fibroins
keywords: Keratin; Oxygen generation; Silk fibroin; Tissue Engineering; Urinary tract