title: Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
abstract: Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction. Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes. Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities. This study was to characterize chemical structures of GLPS3 and determine their effects on diethyldithiocarbamate (DDC)-induced CP in mice. The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively. GLPS3-Ⅰ, GLPS3-Ⅱ and GLPS3-Ⅲ were composed of Glu:Gal:Ara:Xyl, Glu:Gal:Ara:Xyl:Man:Rha, and Glu:Gal:Xyl:Man:Rha:Fuc, with molar ratio of 2.82: 1.33: 1.26: 0.87, 5.84: 2.23: 0.72:1.38: 1.40: 0.51 and 5.34: 2.72: 1.14: 1.10: 0.33: 0.38, respectively. The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides. The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment. Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3. Moreover, interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents were reduced most by GLPS3-Ⅱ. The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
authors.affiliation: College of Resources and Environmental Science, Northeast Agriculture University, Harbin 150030, PR China.
authors.forename: Koukou
authors.initials: K
authors.lastname: Li
authors.affiliation: Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Resources and Environmental Science, Northeast Agriculture University, Harbin 150030, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Resources and Environmental Science, Northeast Agriculture University, Harbin 150030, PR China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Ren
authors.affiliation: College of Resources and Environmental Science, Northeast Agriculture University, Harbin 150030, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zang
authors.affiliation: College of Resources and Environmental Science, Northeast Agriculture University, Harbin 150030, PR China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Resources and Environmental Science, Northeast Agriculture University, Harbin 150030, PR China. Electronic address: juanjuan4050234@163.com.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Qu
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003470:Culture Media; D016207:Cytokines / Q000097:blood; D004050:Ditiocarb / Q000494:pharmacology*; D005285:Fermentation; D051379:Mice; D008813:Mice, Inbred ICR; D010195:Pancreatitis / Q000139:chemically induced / Q000517:prevention & control*; D011134:Polysaccharides / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003470:Culture Media; D016207:Cytokines; D011134:Polysaccharides; D004050:Ditiocarb
keywords: Antioxidant activity; Chronic pancreatitis; Ganoderma lucidum; Polysaccharides