title: The role of MBL2 gene polymorphism in sepsis incidence.
abstract: OBJECTIVE
  This case-control study was aimed to explore the role of mannose-binding lectin 2 (MBL2) gene rs1800450 polymorphism (codon 54 A/B, G230A) in the development of sepsis in Han Chinese.
  
  
  METHODS
  MBL2 rs1800450 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). MBL serum level was detected by enzyme-linked immunosorbent assay (ELISA). Associations between rs1800450 and sepsis susceptibility was detected by Chi-square test and represented by odds ratios (ORs) and 95% confidence intervals (CIs). Correlation of rs1800450 genotypes and MBL serum level was assessed using t test.
  
  
  RESULTS
  Variant A allele frequency was significantly observed in cases than that in controls, indicating a significant association with the susceptibility of sepsis (OR = 1.979, 95% CI = 1.200-3.262). GA genotype also relate to the onset of sepsis (OR = 2.090, 95% CI = 1.163-3.753). MBL serum concentrations were significantly different between case and control groups (P<0.001). Meanwhile, variant allele carriers had lower serum level compared with wild homozygous (P<0.001).
  
  
  CONCLUSIONS
  Variant A allele in MBL2 gene rs1800450 polymorphism might increase the risk of sepsis via decrease the MBL serum level.
authors.affiliation: Intensive Care Unit, Air Force General Hospital of PLA 30 Fucheng Road, Beijing 100142, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Intensive Care Unit, Air Force General Hospital of PLA 30 Fucheng Road, Beijing 100142, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ning
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015994:Incidence; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018805:Sepsis / Q000097:blood / Q000453:epidemiology / Q000235:genetics*
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin
keywords: ELISA; MBL2; PCR-RFLP; Sepsis; polymorphism
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