title: Differential pathlength factor informs evoked stimulus response in a mouse model of Alzheimer's disease.
abstract: Baseline optical properties are typically assumed in calculating the differential pathlength factor (DPF) of mouse brains, a value used in the modified Beer-Lambert law to characterize an evoked stimulus response. We used spatial frequency domain imaging to measure in vivo baseline optical properties in 20-month-old control ([Formula: see text]) and triple transgenic APP/PS1/tau (3xTg-AD) ([Formula: see text]) mouse brains. Average [Formula: see text] for control and 3xTg-AD mice was [Formula: see text] and [Formula: see text], respectively, at 460 nm; and [Formula: see text] and [Formula: see text], respectively, at 530 nm. Average [Formula: see text] for control and 3xTg-AD mice was [Formula: see text] and [Formula: see text], respectively, at 460 nm; and [Formula: see text] and [Formula: see text], respectively, at 530 nm. The calculated DPF for control and 3xTg-AD mice was [Formula: see text] and [Formula: see text] OD mm, respectively, at 460 nm; and [Formula: see text] and [Formula: see text] OD mm, respectively, at 530 nm. In hindpaw stimulation experiments, the hemodynamic increase in brain tissue concentration of oxyhemoglobin was threefold larger and two times longer in the control mice compared to 3xTg-AD mice. Furthermore, the washout of deoxyhemoglobin from increased brain perfusion was seven times larger in controls compared to 3xTg-AD mice ([Formula: see text]).
authors.affiliation: University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Biomedical Engineering, 3120 Natural Sciences II, Irvine, California 92697-2715, United States.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Lin
authors.affiliation: University of California, Irvine , Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States.
authors.forename: Adrien
authors.initials: A
authors.lastname: Ponticorvo
authors.affiliation: University of California, Irvine , Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Durkin
authors.affiliation: University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Chemical Engineering and Materials Science, 916 Engineering Tower, Irvine, California 92697-2575, United States.
authors.forename: Vasan
authors.initials: V
authors.lastname: Venugopalan
authors.affiliation: University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Biomedical Engineering, 3120 Natural Sciences II, Irvine, California 92697-2715, United States; University of California, Irvine, Edwards Lifesciences Center for Advanced Cardiovascular Technology, 2400 Engineering Hall, Irvine, California 92697-2730, United States.
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
authors.affiliation: University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Biomedical Engineering, 3120 Natural Sciences II, Irvine, California 92697-2715, United States.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Tromberg
keywords: LED microprojector; absorption; cerebral metabolic rate of oxygen; functional activation; scattering; spatial frequency domain imaging; tissue optics
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