title: Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status.
abstract: Objectives. The relationship among matriptase function, cellular redox status, and maintenance of intestinal barrier integrity has not been established yet. The aim of this study is to reveal if the crosstalk between matriptase activators and intestinal epithelial monolayers can lead to perturbations in physiological redox regulation in vitro. Methods. The effects of suramin and sphingosine-1-phosphate (S1P) were tested on viability of intestinal porcine epithelial IPEC-J2 cells using MTS assay. Measurements of transepithelial electrical resistance (TER) were performed to determine changes in barrier integrity of cell monolayers. Amplex Red assay was used to monitor extracellular hydrogen peroxide production. Occludin distribution pattern was detected prior to and after matriptase activation using immunofluorescent staining technique. Results. TER reduction was observed in suramin-treated IPEC-J2 cell monolayers, which could be attributed to cell cytotoxic properties of 48 hr 50 μM suramin administration. In contrast, S1P treatment increased TER significantly and elevated occludin accumulation in tight junctions. It was also found that extracellular hydrogen peroxide levels were maintained in IPEC-J2 cells exposed to matriptase activators. Discussion. S1P administration not accompanied by redox imbalance might be one of the key strategies in the improvement of barrier function and consequently in the therapy of intestinal inflammations.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Pászti-Gere
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary.
authors.forename: Á
authors.initials: Á
authors.lastname: Jerzsele
authors.affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Üllői ut 26, Budapest 1085, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Balla
authors.affiliation: Faculty of Pharmacy, Semmelweis University, Hőgyes u. 7, Budapest 1092, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Ujhelyi
authors.affiliation: Agro-Environmental Research Institute, National Agricultural Research and Innovation Centre, Herman O. u. 15, Budapest 1022, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Székács
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017097:Electric Impedance; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006861:Hydrogen Peroxide / Q000378:metabolism; D008246:Lysophospholipids / Q000494:pharmacology*; D062793:Occludin / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013498:Suramin / Q000494:pharmacology; D034421:Sus scrofa
chemical_list: D008246:Lysophospholipids; D062793:Occludin; C060506:sphingosine 1-phosphate; D013498:Suramin; D006861:Hydrogen Peroxide; D012697:Serine Endopeptidases; C485595:matriptase; D013110:Sphingosine
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