title: High-Resolution DNA Melting Analysis in Plant Research.
abstract: Genetic and genomic studies provide valuable insight into the inheritance, structure, organization, and function of genes. The knowledge gained from the analysis of plant genes is beneficial to all aspects of plant research, including crop improvement. New methods and tools are continually being developed to facilitate rapid and accurate mapping, sequencing, and analyzing of genes. Here, I review the recent progress in the application of high-resolution melting (HRM) analysis of DNA, a method that allows detecting polymorphism in double-stranded DNA by comparing profiles of melting curves. Use of HRM has expanded considerably in the past few years as the method was successfully applied for high-throughput genotyping, mapping genes, testing food products and seeds, and other areas of plant research.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, U.S. Agricultural Research Station, 1636 E. Alisal St, Salinas, CA 93905, USA. Electronic address: Ivan.Simko@ars.usda.gov.
authors.forename: Ivan
authors.initials: I
authors.lastname: Simko
mesh_terms: D002874:Chromosome Mapping; D016172:DNA Fingerprinting / Q000379:methods; D017343:Genes, Plant; D005819:Genetic Markers; D023281:Genomics / Q000379:methods / Q000639:trends; D009691:Nucleic Acid Denaturation; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000639:trends; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D005819:Genetic Markers
keywords: barcoding; fingerprinting; genotyping; marker-assisted selection; mutation detection