title: Hyperspectral optical tomography of intrinsic signals in the rat cortex.
abstract: We introduce a tomographic approach for three-dimensional imaging of evoked hemodynamic activity, using broadband illumination and diffuse optical tomography (DOT) image reconstruction. Changes in diffuse reflectance in the rat somatosensory cortex due to stimulation of a single whisker were imaged at a frame rate of 5 Hz using a hyperspectral image mapping spectrometer. In each frame, images in 38 wavelength bands from 484 to 652 nm were acquired simultaneously. For data analysis, we developed a hyperspectral DOT algorithm that used the Rytov approximation to quantify changes in tissue concentration of oxyhemoglobin ([Formula: see text]) and deoxyhemoglobin (ctHb) in three dimensions. Using this algorithm, the maximum changes in [Formula: see text] and ctHb were found to occur at [Formula: see text] and [Formula: see text] beneath the surface of the cortex, respectively. Rytov tomographic reconstructions revealed maximal spatially localized increases and decreases in [Formula: see text] and ctHb of [Formula: see text] and [Formula: see text], respectively, with these maximum changes occurring at [Formula: see text] poststimulus. The localized optical signals from the Rytov approximation were greater than those from modified Beer-Lambert, likely due in part to the inability of planar reflectance to account for partial volume effects.
authors.affiliation: University of California, Irvine , Beckman Laser Institute and Medical Clinic, Laser Microbeam and Medical Program, 1002 Health Sciences Road, Irvine, California 92612, United States.
authors.forename: Soren D
authors.initials: SD
authors.lastname: Konecky
authors.affiliation: University of California, Irvine , Beckman Laser Institute and Medical Clinic, Laser Microbeam and Medical Program, 1002 Health Sciences Road, Irvine, California 92612, United States.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Wilson
authors.affiliation: Rice University , Department of Biomedical Engineering, 6500 Main Street, Houston, Texas 77030, United States.
authors.forename: Nathan
authors.initials: N
authors.lastname: Hagen
authors.affiliation: University of California, Irvine, Beckman Laser Institute and Medical Clinic, Laser Microbeam and Medical Program, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Biomedical Engineering, 5200 Engineering Hall, Irvine, California 92697, United States.
authors.forename: Amaan
authors.initials: A
authors.lastname: Mazhar
authors.affiliation: Rice University , Department of Biomedical Engineering, 6500 Main Street, Houston, Texas 77030, United States.
authors.forename: Tomasz S
authors.initials: TS
authors.lastname: Tkaczyk
authors.affiliation: University of California, Irvine, Department of Neurobiology and Behavior, 2205 McGaugh Hall, Irvine, California 92697, United States; University of California, Irvine, Department of Biomedical Engineering, 5200 Engineering Hall, Irvine, California 92697, United States.
authors.forename: Ron D
authors.initials: RD
authors.lastname: Frostig
authors.affiliation: University of California, Irvine, Beckman Laser Institute and Medical Clinic, Laser Microbeam and Medical Program, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Biomedical Engineering, 5200 Engineering Hall, Irvine, California 92697, United States.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Tromberg
keywords: hemodynamic imaging; hyperspectral imaging; neurovascular coupling; optical brain imaging; optical tomography; wide-field optical imaging
references.citation: J Neurophysiol. 2008 May;99(5):2035-47
references.pmid: 17913979
references.citation: Neuroimage. 2001 Jun;13(6 Pt 1):988-1001
references.pmid: 11352605
references.citation: Opt Express. 2008 Mar 31;16(7):5048-60
references.pmid: 18542605
references.citation: J Biomed Opt. 2011 Jan-Feb;16(1):016013
references.pmid: 21280919
references.citation: J Cereb Blood Flow Metab. 2005 Jul;25(7):830-41
references.pmid: 15744249
references.citation: Stroke. 2010 Oct;41(10 Suppl):S144-6
references.pmid: 20876491
references.citation: Nat Methods. 2010 Sep;7(9):755-9
references.pmid: 20693997
references.citation: Nat Rev Neurosci. 2004 May;5(5):347-60
references.pmid: 15100718
references.citation: Appl Opt. 2010 Apr 1;49(10 ):1886-99
references.pmid: 20357875
references.citation: Proc Natl Acad Sci U S A. 1986 Feb;83(4):1140-4
references.pmid: 3485282
references.citation: Neuroimage. 2011 Jan 15;54(2):1021-30
references.pmid: 20858545
references.citation: Phys Med Biol. 2001 Mar;46(3):879-96
references.pmid: 11277232
references.citation: Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):9998-10002
references.pmid: 8234348
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2014 Feb 1;31(2):301-11
references.pmid: 24562029
references.citation: J Biomed Opt. 2012 May;17 (5):056008
references.pmid: 22612131
references.citation: J Neurosci. 2007 Apr 25;27(17):4572-86
references.pmid: 17460070
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2002 Mar;19(3):558-66
references.pmid: 11876321
references.citation: Opt Lett. 2003 Jan 1;28(1):28-30
references.pmid: 12656525
references.citation: J Biomed Opt. 2002 Jul;7(3):464-70
references.pmid: 12175298
references.citation: Opt Lett. 2005 Dec 15;30(24):3338-40
references.pmid: 16389824
references.citation: Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18390-5
references.pmid: 19828443
references.citation: Front Neuroenergetics. 2010 Jun 14;2:12
references.pmid: 20616883
references.citation: Opt Express. 2008 May 12;16(10):7435-46
references.pmid: 18545448
references.citation: Proc Natl Acad Sci U S A. 1990 Aug;87(16):6082-6
references.pmid: 2117272
references.citation: Phys Med Biol. 2003 May 21;48(10):1391-403
references.pmid: 12812454
references.citation: Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15246-51
references.pmid: 20696904
references.citation: J Biomed Opt. 2005 Jan-Feb;10(1):11013
references.pmid: 15847579
references.citation: Biophys J. 2001 Feb;80(2):986-93
references.pmid: 11159465
references.citation: Science. 1991 Nov 1;254(5032):716-9
references.pmid: 1948051
references.citation: Opt Express. 2009 Aug 17;17(17):14780-90
references.pmid: 19687956
references.citation: Eur Radiol. 2007 Nov;17(11):2926-33
references.pmid: 17473923
references.citation: Neuroimage. 2007 Mar;35(1):89-104
references.pmid: 17222567
references.citation: Opt Express. 2010 Jul 5;18(14):14330-44
references.pmid: 20639917
references.citation: Opt Lett. 2006 Dec 1;31(23):3459-61
references.pmid: 17099749
references.citation: Opt Lett. 2004 Jul 1;29(13):1506-8
references.pmid: 15259728
references.citation: Stroke. 2003 Sep;34(9):2279-84
references.pmid: 12881609
references.citation: J Biomed Opt. 2009 Mar-Apr;14(2):024033
references.pmid: 19405762
references.citation: Neuroimage. 2006 Apr 1;30(2):521-8
references.pmid: 16246586
references.citation: Neuroimage. 2001 Jun;13(6 Pt 1):1002-15
references.pmid: 11352606
references.citation: Opt Express. 2007 Oct 17;15(21):14013-27
references.pmid: 19550674
references.citation: Neuroimage. 2007;37 Suppl 1:S27-36
references.pmid: 17574868
references.citation: Opt Lett. 2009 Aug 15;34(16):2477-9
references.pmid: 19684821
references.citation: Opt Express. 2001 Sep 10;9(6):272-86
references.pmid: 19421298
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12169-74
references.pmid: 17616584
references.citation: Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):13081-6
references.pmid: 14569029
references.citation: Neuroimage. 2012 Feb 15;59(4):3201-11
references.pmid: 22155031
references.citation: Nature. 1986 Nov 27-Dec 3;324(6095):361-4
references.pmid: 3785405
references.citation: Science. 1996 Apr 26;272(5261):551-4
references.pmid: 8614805
references.citation: Opt Express. 2009 Jul 20;17(15):12293-308
references.pmid: 19654631
references.citation: Neuroimage. 2009 Aug;47 Suppl 2:T36-43
references.pmid: 19013531
references.citation: Front Neuroanat. 2011 May 16;5:29
references.pmid: 21647212
references.citation: Opt Express. 2008 Jun 9;16(12 ):8581-93
references.pmid: 18545571
references.citation: Phys Med Biol. 2005 Feb 21;50(4):R1-43
references.pmid: 15773619
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15741-6
references.pmid: 9861040
references.citation: J Cereb Blood Flow Metab. 2003 Aug;23(8):911-24
references.pmid: 12902835