title: Validation of Urine Test for Detection of Helicobacter pylori Infection in Indonesian Population.
abstract: We measured the accuracy of the urine test (RAPIRUN) for detection of Helicobacter pylori infection in Indonesia (Jakarta, Pontianak, and Jayapura) using histology confirmed by immunohistochemistry and/or culture as gold standards. We also used immunohistochemistry to identify CagA phenotype and analyzed H. pylori CagA diversity in Indonesia. The overall prevalence of H. pylori infection in 88 consecutive dyspeptic patients based on the urine test was 15.9% (14/88), 38.1% for patients in Jayapura that had higher prevalence of H. pylori infection than that in Jakarta (9.7%, P = 0.02) and Pontianak (8.3%, P = 0.006). Overall sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of RAPIRUN were 83.3%, 94.7%, 71.4%, 97.3%, and 93.2%, respectively. All of the H. pylori-positive patients were immunoreactive for anti-CagA antibody but not immunoreactive for East Asian specific anti-CagA antibody in all H. pylori-positive subjects. We confirmed the high accuracy of RAPIRUN in Indonesian population. In general, we found less virulent type of H. pylori in Indonesia, which partly explained the low incidence gastric cancer in Indonesia.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Ari Fahrial
authors.initials: AF
authors.lastname: Syam
authors.affiliation: Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, Japan; Gastroentero-Hepatology Division, Department of Internal Medicine, Airlangga University Faculty of Medicine, Surabaya 60131, Indonesia; Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Miftahussurur
authors.affiliation: Department of Internal Medicine, Santo Antonius Hospital, Pontianak 78115, Indonesia.
authors.forename: Willy Brodus
authors.initials: WB
authors.lastname: Uwan
authors.affiliation: Department of Internal Medicine, Yowari Hospital, Jayapura 99352, Indonesia.
authors.forename: David
authors.initials: D
authors.lastname: Simanjuntak
authors.affiliation: Department of Molecular Pathology, Oita University Faculty of Medicine, Yufu 879-5593, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Uchida
authors.affiliation: Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, Japan; Department of Gastroenterology and Hepatology, Baylor College of Medicine and Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX 77030, USA.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D004415:Dyspepsia / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000652:urine*; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000652:urine*; D016480:Helicobacter pylori; D006801:Humans; D007214:Indonesia; D008297:Male; D008875:Middle Aged
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori
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