title: Rapid and accurate tumor-target bio-imaging through specific in vivo biosynthesis of a fluorescent europium complex.
abstract: A new and facile method for rapidly and accurately achieving tumor targeting fluorescent images has been explored using a specifically biosynthesized europium (Eu) complex in vivo and in vitro. It demonstrated that a fluorescent Eu complex could be bio-synthesized through a spontaneous molecular process in cancerous cells and tumors, but not prepared in normal cells and tissues. In addition, the proteomics analyses show that some biological pathways of metabolism, especially for NADPH production and glutamine metabolism, are remarkably affected during the relevant biosynthesis process, where molecular precursors of europium ions are reduced to fluorescent europium complexes inside cancerous cells or tumor tissues. These results proved that the specific self-biosynthesis of a fluorescent Eu complex by cancer cells or tumor tissues can provide a new strategy for accurate diagnosis and treatment strategies in the early stages of cancers and thus is beneficial for realizing precise surgical intervention based on the relevant cheap and readily available agents.
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Lab) School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China. xuewang@seu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.forename: Jianling
authors.initials: J
authors.lastname: Wang
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Li
authors.forename: Xiawei
authors.initials: X
authors.lastname: Dong
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Xuerui
authors.initials: X
authors.lastname: Jiang
authors.forename: Hongde
authors.initials: H
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005456:Fluorescent Dyes; D005063:Europium