title: Biosynthesis, characterization and antimicrobial action of silver nanoparticles from root bark extract of Berberislycium Royle.
abstract: Various biological methods are being recognized for the fabrication of silver nanoparticles, which are used in several fields. The phytosynthesis of nanoparticles came out as a cost effective and enviro-friendly approach. When root bark extract of Berberis lycium was treated with silver ions, they reduced to silver nanoparticles, which were spherical, crystalline, size ranged from 10-100nm and capped by biomolecules. Synthesized silver nanoparticles were characterized by UV-visible spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) and Fourier Transform Infra Red Spectroscopy (FTIR). The plant mediated synthesized silver nanoparticles showed pronounced antimicrobial activities against both Gram negative bacteria (Escherichia coli, Klebseilla pneumoniae, Pseudomonas aeruginosa) and Gram positive bacteria (Staphylococcus aureus and Bacillus subtilis). The plant mediated process proved to be non-toxic and low cost contender as reducing agent for synthesizing stable silver nanoparticles.
authors.affiliation: Department of Botany, City Campus, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
authors.forename: Ansar
authors.initials: A
authors.lastname: Mehmood
authors.affiliation: Department of Botany, City Campus, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
authors.forename: Ghulam
authors.initials: G
authors.lastname: Murtaza
authors.affiliation: Department of Chemical Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.
authors.forename: Tariq Mahmood
authors.initials: TM
authors.lastname: Bhatti
authors.affiliation: Department of Chemical Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.
authors.forename: Rehana
authors.initials: R
authors.lastname: Kausar
authors.affiliation: Department of Botany, City Campus, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
authors.forename: Muhammad Jamil
authors.initials: MJ
authors.lastname: Ahmed
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D028142:Berberis; D053768:Metal Nanoparticles; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D012834:Silver / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D012834:Silver