title: Effects of the SLCO1B1 *1 and SLCO1B1 *5 polymorphisms on IL-6 and IL-10 levels in patients under pravastatin treatment prior to inguinal hernia repair.
abstract: BACKGROUND
  Different genetic variants in the SLCO1B1 gene have been shown to have functional importance in individual variability in pravastatin pharmacokinetics, resulting in different inflammatory responses to surgical inguinal hernia repair. The aim of this study was to determine IL-6 and IL-10 serum concentrations in the presence and absence of the SLCO1B1*1 and SLCO1B1*5 polymorphisms in patients under pravastatin treatment that underwent inguinal hernia repair.
  
  
  METHODS
  The study included 26 subjects that were under pravastatin treatment (40 mg/day) at least 1 month prior to inguinal hernia repair open technique. All the subjects were genotyped for the SLCO1B1*1 and SLCO1B1*5 polymorphisms through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and their preoperative and postoperative serum IL-6 and IL-10 levels were quantified through the ELISA technique. The IL-6 and IL-10 levels were analyzed in the presence or absence of the mutated polymorphism for SLCO1B1*1 and SLCO1B1*5.
  
  
  RESULTS
  The SLCO1B1*1 polymorphism had a frequency of 38.5% and the SLCO1B1*5 polymorphism had a frequency of 19.2%. The preoperative and postoperative serum concentrations of IL-6 were 0.252 pg/ml ± 0.19 and 0.206 pg/ml ± 0.20, respectively, with a p = 0.525, whereas the preoperative and postoperative serum concentrations for IL-10 were 4.943 pg/ml ± 3.13 and 4.611 pg/ml ± 3.01, respectively, with a p = 0.004.
  
  
  CONCLUSIONS
  The patients under pravastatin treatment presented with lower postoperative IL-10 levels with respect to the baseline concentration (p = 0.004), regardless of the presence or absence of the two polymorphisms.
authors.affiliation: Hospital General de Zona UMF No.1 IMSS, Colima, Mexico. Electronic address: cardenas_r@gmail.com.
authors.forename: Martha Irazema
authors.initials: MI
authors.lastname: Cárdenas-Rojas
authors.affiliation: Facultad de Medicina, Universidad de Colima, Colima, Mexico. Electronic address: ivancoliman@hotmail.com.
authors.forename: Ivan
authors.initials: I
authors.lastname: Delgado-Enciso
authors.affiliation: Facultad de Medicina, Universidad de Colima, Colima, Mexico. Electronic address: luzma_br2004@yahoo.es.
authors.forename: Luz Margarita
authors.initials: LM
authors.lastname: Baltazar-Rodríguez
authors.affiliation: Hospital General de Zona UMF No.1 IMSS, Colima, Mexico. Electronic address: pepeguzman_esquivel@outlook.com.
authors.forename: José
authors.initials: J
authors.lastname: Guzmán-Esquivel
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Mexico. Electronic address: mario_ramirez@ucol.mx.
authors.forename: Mario
authors.initials: M
authors.lastname: Ramírez-Flores
mesh_terms: D000368:Aged; D000924:Anticholesteremic Agents / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006552:Hernia, Inguinal / Q000235:genetics* / Q000601:surgery; D059685:Herniorrhaphy / Q000009:adverse effects; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D027381:Liver-Specific Organic Anion Transporter 1 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011183:Postoperative Complications / Q000097:blood / Q000235:genetics; D011184:Postoperative Period; D017035:Pravastatin / Q000627:therapeutic use; D057234:Preoperative Period
chemical_list: D000924:Anticholesteremic Agents; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D027381:Liver-Specific Organic Anion Transporter 1; C503999:SLCO1B1 protein, human; D016753:Interleukin-10; D017035:Pravastatin
keywords: Interleukin-10; Interleukin-6; Polymorphisms SLCO1B1 *1; Pravastatin and inguinal hernia; SLCO1B1 *5