title: Gata2 Is a Rheostat for Mesenchymal Stem Cell Fate in Male Mice.
abstract: Gata2 is a zinc finger transcription factor that is important in hematopoiesis and neuronal development. However, the roles of Gata2 in the mesenchymal lineages are poorly understood. In vitro studies suggest that Gata2 modulates adipocyte differentiation and mesenchymal stem cell (MSC) proliferation. To systematically determine the in vivo functions of Gata2 in the MSC lineage commitment and development, we have generated three mouse models in which Gata2 is specifically deleted in MSCs, adipocytes, or osteoblasts. During the MSC expansion stage, Gata2 promotes proliferation and attenuates differentiation; thereby Gata2 loss in MSCs results in enhanced differentiation of both adipocytes and osteoblasts. During the differentiation stage, Gata2 also plays MSC-independent roles to impede lineage commitment; hence, Gata2 loss in adipocyte or osteoblast lineages also augments adipogenesis and osteoblastogenesis, respectively. These findings reveal Gata2 as a crucial rheostat of MSC fate to control osteoblast and adipocyte lineage development.
authors.affiliation: Department of Pharmacology (X.L., H.H., H.Z., Y.W.), The University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Veterinary Biosciences (M.S.), University of Helsinki, Helsinki 00014, Finland.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacology (X.L., H.H., H.Z., Y.W.), The University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Veterinary Biosciences (M.S.), University of Helsinki, Helsinki 00014, Finland.
authors.forename: HoangDinh
authors.initials: H
authors.lastname: Huynh
authors.affiliation: Department of Pharmacology (X.L., H.H., H.Z., Y.W.), The University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Veterinary Biosciences (M.S.), University of Helsinki, Helsinki 00014, Finland.
authors.forename: Hao
authors.initials: H
authors.lastname: Zuo
authors.affiliation: Department of Pharmacology (X.L., H.H., H.Z., Y.W.), The University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Veterinary Biosciences (M.S.), University of Helsinki, Helsinki 00014, Finland.
authors.forename: Marjo
authors.initials: M
authors.lastname: Salminen
authors.affiliation: Department of Pharmacology (X.L., H.H., H.Z., Y.W.), The University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Veterinary Biosciences (M.S.), University of Helsinki, Helsinki 00014, Finland.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Wan
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage; D049109:Cell Proliferation / Q000235:genetics*; D050989:GATA2 Transcription Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse
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