title: Detection of Autophagy in Caenorhabditis elegans.
abstract: Autophagy is a dynamic and catabolic process that results in the breakdown and recycling of cellular components through the autophagosomal-lysosomal pathway. Many autophagy genes identified in yeasts and mammals have orthologs in the nematode Caenorhabditis elegans. In recent years, gene inactivation by RNA interference (RNAi) and chromosomal mutations has been useful to probe the functions of autophagy in C. elegans, and a role for autophagy has been shown to contribute to multiple processes, such as the adaptation to stress, longevity, cell death, cell growth control, clearance of aggregation-prone proteins, degradation of P granules during embryogenesis, and apoptotic cell clearance. Here, we discuss some of these roles and describe methods that can be used to study autophagy in C. elegans. Specifically, we summarize how to visualize autophagy in embryos, larva, or adults, how to detect the lipidation of the ubiquitin-like modifier LGG-1 by western blot, and how to inactivate autophagy genes by RNAi.
authors.affiliation: Department of Biology, Queens College-CUNY, Flushing, New York 11367; The Graduate Center, The City University of New York, New York 10016.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Palmisano
authors.affiliation: Department of Biology, Queens College-CUNY, Flushing, New York 11367; The Graduate Center, The City University of New York, New York 10016.
authors.forename: Alicia
authors.initials: A
authors.lastname: Meléndez
mesh_terms: D000818:Animals; D001343:Autophagy; D015153:Blotting, Western / Q000379:methods; D017173:Caenorhabditis elegans / Q000502:physiology*; D055785:Gene Knockdown Techniques; D008967:Molecular Biology / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D034622:RNA Interference
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