title: Role and mechanism of microRNA-21 in H2O2-induced apoptosis in bone marrow mesenchymal stem cells.
abstract: microRNA-21 (miR-21) contributes to anti-apoptosis, proliferation and migration in many cells, but its role in inhibiting apoptosis in bone marrow mesenchymal stem cells (BMSC) remains unclear. The aim of this study was to determine the role of miR-21 in H2O2-induced BMSC apoptosis. We used quantitative real time-polymerase chain reaction (RT-PCR) to demonstrate the level of miR-21 after treatment of BMSC with H2O2. BMSC apoptosis was induced by different concentrations of H2O2 and was decreased in miR-21-upregulated cells. The expression of PTEN, a functional target gene of miR-21 in BMSC, was regulated by miR-21. The RT-PCR results indicated that miR-21 was significantly up-regulated, and western blot analysis indicated that Bcl-2 was up-regulated, whereas the apoptosis-related genes caspase 3/9 and Bax were down-regulated in miR-21-up-regulated cells. The miR-21-up-regulated cells had significantly enhanced Akt phosphorylation, as measured by western blot analysis. LY294002, an inhibitor of Akt activation, abolished the protective effects of miR-21-up-regulated cells. These results suggest that miR-21 contributes to inhibition of apoptosis in BMSC by down-regulating PTEN, potentially via the PI3K/Akt pathway.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Street, Heping District, Shenyang 110001, Liaoning, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Lv
authors.affiliation: Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Yuehan
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Street, Heping District, Shenyang 110001, Liaoning, China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Street, Heping District, Shenyang 110001, Liaoning, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Street, Heping District, Shenyang 110001, Liaoning, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cong
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Street, Heping District, Shenyang 110001, Liaoning, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Street, Heping District, Shenyang 110001, Liaoning, China. Electronic address: tu188@sina.com.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Tu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects*; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D002867:Chromones / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D035683:MicroRNAs / Q000235:genetics*; D009025:Morpholines / Q000494:pharmacology; D051059:PTEN Phosphohydrolase / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: D020169:Caspases; D002867:Chromones; D004791:Enzyme Inhibitors; D006861:Hydrogen Peroxide; D035683:MicroRNAs; D009025:Morpholines; D051059:PTEN Phosphohydrolase; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; D051028:bcl-2-Associated X Protein; C494826:BAX protein, human; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C494929:PTEN protein, human
keywords: Apoptosis; BMSC; H(2)O(2); PTEN; miR-21; p-Akt