title: Role of Periostin in Adhesion and Migration of Bone Remodeling Cells.
abstract: Periostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key roles in the maintenance of dental tissues and it is mainly expressed in those areas where tension or pressing forces are taking place. In this regard, high expression of periostin is essential to promote migration and proliferation of periodontal ligament fibroblasts. However little is known about the participation of periostin in migration and adhesion processes of bone remodeling cells. In this work we employ the mouse pre-osteoblastic MC3T3-E1 and the macrophage-like RAW 264.7 cell lines to overexpress periostin and perform different cell-based assays to study changes in cell behavior. Our data indicate that periostin overexpression not only increases adhesion capacity of MC3T3-E1 cells to different matrix proteins but also hampers their migratory capacity. Changes on RNA expression profile of MC3T3-E1 cells upon periostin overexpression have been also analyzed, highlighting the alteration of genes implicated in processes such as cell migration, adhesion or bone metabolism but not in bone differentiation. Overall, our work provides new evidence on the impact of periostin in osteoblasts physiology.
authors.affiliation: Departamento de Cirugía y Especialidades Médico-Quirúrgicas, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cobo
authors.affiliation: Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Cristina G
authors.initials: CG
authors.lastname: Viloria
authors.affiliation: Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Solares
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Tania
authors.initials: T
authors.lastname: Fontanil
authors.affiliation: Departamento de Cirugía y Especialidades Médico-Quirúrgicas, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: González-Chamorro
authors.affiliation: Departamento de Cirugía y Especialidades Médico-Quirúrgicas, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: De Carlos
authors.affiliation: Departamento de Cirugía y Especialidades Médico-Quirúrgicas, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Cobo
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.|Instituto Universitario de Oncología (IUOPA), Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Cal
authors.affiliation: Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.|Instituto Universitario de Oncología (IUOPA), Facultad de Medicina, Universidad de Oviedo, Asturias, Spain.
authors.forename: Alvaro J
authors.initials: AJ
authors.lastname: Obaya
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000235:genetics / Q000502:physiology; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D051379:Mice
chemical_list: D015815:Cell Adhesion Molecules; C082838:Postn protein, mouse
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