title: [Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci].
abstract: OBJECTIVE
  To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.
  
  
  METHODS
  Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.
  
  
  RESULTS
  A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.
  
  
  CONCLUSIONS
  The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Shuang-shuang
authors.initials: SS
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Wu
authors.forename: Shu-qin
authors.initials: SQ
authors.lastname: Huang
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Zheng
authors.forename: Fa-yuan
authors.initials: FY
authors.lastname: Li
authors.forename: Bin-wen
authors.initials: BW
authors.lastname: Ge
authors.forename: Yu-lin
authors.initials: YL
authors.lastname: Guo
authors.forename: Huai-gu
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D053523:Amelogenin; D041322:Chromosomes, Human, Y / Q000235:genetics; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D053803:Forensic Genetics / Q000379:methods; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation*; D006801:Humans; D007202:Indicators and Reagents; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction
chemical_list: D053523:Amelogenin; D017931:DNA Primers; D007202:Indicators and Reagents