title: Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell.
abstract: In this paper, the construction of 3D model structure of xylose dehydrogenase (XDH) by using homology modeling to guide the rational design of the enzyme for improving thermostability was reported. Three XDH mutants of NA-1 (+249L), NA-2 (G149P) and NA-3 (+249L/G149P) were designed and displayed on the surface of bacteria. Among them, bacteria displaying NA-1 (NA-1-bacteria) exhibited superior thermostability without compromising its activity and substrate specificity in comparison with its wild-type counterpart. NA-1-bacteria retained its original activity after incubation at room temperature for one-month with the half-life of 9.8 days at 40°C. Finally, the NA-1-bacteria were applied to construct xylose/O2 based biofuel cell with good performance including enhanced operational stability. Thus, the approach described here could be explored for engineering of other enzymes for improving certain characters without three-dimensional structure identified by experimental methods.
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education, and College of Chemistry and Chemical Engineering, Ocean University of China, 238 Songling Road, Qingdao 266100, China; Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Feng
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors.forename: Chuantao
authors.initials: C
authors.lastname: Hou
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors.forename: Dongfei
authors.initials: D
authors.lastname: Han
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors.forename: Qiaolin
authors.initials: Q
authors.lastname: Lang
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China. Electronic address: liuah@qibebt.ac.cn.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education, and College of Chemistry and Chemical Engineering, Ocean University of China, 238 Songling Road, Qingdao 266100, China. Electronic address: lhhan@ouc.edu.cn.
authors.forename: Lihui
authors.initials: L
authors.lastname: Han
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001674:Bioelectric Energy Sources; D004795:Enzyme Stability; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C035795:D-xylose dehydrogenase
keywords: Homology modeling; Microbial surface display; Thermostability; Xylose dehydrogenase mutants; Xylose/O(2) based biofuel cell