title: Species Specificity of the Putative Male Antennal Aphrodisiac Pheromone in Leptopilina heterotoma, Leptopilina boulardi, and Leptopilina victoriae.
abstract: Male antennal aphrodisiac pheromones have been suggested to elicit female receptiveness in several parasitic Hymenoptera, including Leptopilina boulardi. None of the proposed pheromones, however, has been fully identified to date. It is also unknown whether these antennal pheromones are species specific, because the species specificity of mate recognition and courtship elicitation in Leptopilina prevented such experiments. In this study we present an experimental design that allows the investigation of the species specificity of the putative male aphrodisiac pheromone of L. heterotoma, L. boulardi, and L. victoriae. This is achieved by chemical manipulation of the odour profile of heterospecific females, so that males perceive them as conspecifics and show antennal courtship behaviour. Males courted the manipulated heterospecific females and antennal contact between the male and the female was observed. However, males elicited receptiveness only in conspecific females, never in the manipulated heterospecific females. Chemical analysis showed the presence of species specific unsaturated hydrocarbons on the antennae of males. Only trace amounts of these hydrocarbons are found on the antennae of females. Our results are an important step towards the understanding and identification of antennal pheromones of parasitic wasps.
authors.affiliation: Institute for Zoology, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany; Institute of Biology, Free University of Berlin, Haderslebener Straße 9, 12163 Berlin, Germany.
authors.forename: Ingmar
authors.initials: I
authors.lastname: Weiss
authors.affiliation: Institute for Zoology, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Ruther
authors.affiliation: Institute for Zoology, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Stökl
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D012724:Sex Attractants / Q000378:metabolism*; D013045:Species Specificity; D014863:Wasps / Q000378:metabolism*
chemical_list: D012724:Sex Attractants
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