title: In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography.
abstract: Muscle atrophy is a widespread ill condition occurring in many diseases, which can reduce quality of life and increase morbidity and mortality. We developed a new method using non-invasive ultrasonography to measure soleus and gastrocnemius lateralis muscle atrophy in the hindlimb-unloaded rat, a well-accepted model of muscle disuse. Soleus and gastrocnemius volumes were calculated using the conventional truncated-cone method and a newly-designed sinusoidal method. For Soleus muscle, the ultrasonographic volume determined in vivo with either method was linearly correlated to the volume determined ex-vivo from excised muscles as muscle weight-to-density ratio. For both soleus and gastrocnemius muscles, a strong linear correlation was obtained between the ultrasonographic volume and the muscle fiber cross-sectional area determined ex-vivo on muscle cryosections. Thus ultrasonography allowed the longitudinal in vivo evaluation of muscle atrophy progression during hindlimb unloading. This study validates ultrasonography as a powerful method for the evaluation of rodent muscle atrophy in vivo, which would prove useful in disease models and therapeutic trials.
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Mele
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Fonzino
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Rana
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Giulia Maria
authors.initials: GM
authors.lastname: Camerino
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: De Bellis
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Conte
authors.affiliation: Department of Biomedical Sciences &Human Oncology, Polyclinic Biological Research Institute, University of Bari Aldo Moro, P.zza Giulio Cesare 11, Bari, 70124 Italy.
authors.forename: Arcangela
authors.initials: A
authors.lastname: Giustino
authors.affiliation: Section of Pharmacology, Department of Pharmacy &Drug Sciences, University of Bari Aldo Moro, Bari, 70125 Italy.
authors.forename: Diana
authors.initials: D
authors.lastname: Conte Camerino
authors.affiliation: Department of Biomedical Sciences &Human Oncology, Polyclinic Biological Research Institute, University of Bari Aldo Moro, P.zza Giulio Cesare 11, Bari, 70124 Italy.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Desaphy
mesh_terms: D000818:Animals; D008297:Male; D018482:Muscle, Skeletal / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D009133:Muscular Atrophy / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D014463:Ultrasonography
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