title: Novel functional microRNAs from virus-free and infected Vitis vinifera plants under water stress.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that regulate the post-transcriptional control of several pathway intermediates, thus playing pivotal roles in plant growth, development and response to biotic and abiotic stresses. In recent years, the grapevine genome release, small(s)-RNAseq and degradome-RNAseq together has allowed the discovery and characterisation of many miRNA species, thus rendering the discovery of additional miRNAs difficult and uncertain. Taking advantage of the miRNA responsiveness to stresses and the availability of virus-free Vitis vinifera plants and those infected only by a latent virus, we have analysed grapevines subjected to drought in greenhouse conditions. The sRNA-seq and other sequence-specific molecular analyses have allowed us to characterise conserved miRNA expression profiles in association with specific eco-physiological parameters. In addition, we here report 12 novel grapevine-specific miRNA candidates and describe their expression profile. We show that latent viral infection can influence the miRNA profiles of V. vinifera in response to drought. Moreover, study of eco-physiological parameters showed that photosynthetic rate, stomatal conductance and hydraulic resistance to water transport were significantly influenced by drought and viral infection. Although no unequivocal cause-effect explanation could be attributed to each miRNA target, their contribution to the drought response is discussed.
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Research Unit of Bari. Via Amendola 165/a, 70126 Bari, Italy.
authors.forename: Vitantonio
authors.initials: V
authors.lastname: Pantaleo
authors.affiliation: Department of Agricultural, Forest and Food Sciences, University of Torino. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Vitali
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Grugliasco Unit. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Boccacci
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Torino. Strada delle Cacce 73, 10135 Torino, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Miozzi
authors.affiliation: Department of Agricultural, Forest and Food Sciences, University of Torino. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Danila
authors.initials: D
authors.lastname: Cuozzo
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Grugliasco Unit. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Walter
authors.initials: W
authors.lastname: Chitarra
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Grugliasco Unit. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Mannini
authors.affiliation: Department of Agricultural, Forest and Food Sciences, University of Torino. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.|Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Grugliasco Unit. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Lovisolo
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Grugliasco Unit. Largo P. Braccini 2, 10095 Grugliasco-TO, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Gambino
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017124:Conserved Sequence; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D054046:Plant Stomata / Q000502:physiology; D010942:Plant Viruses / Q000502:physiology*; D020871:RNA Stability / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012987:Soil; D013312:Stress, Physiological / Q000235:genetics*; D027843:Vitis / Q000235:genetics* / Q000502:physiology / Q000821:virology*; D014867:Water
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D012987:Soil; D014867:Water
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