title: The Coadministration of N-Acetylcysteine Ameliorates the Effects of Arsenic Trioxide on the Male Mouse Genital System.
abstract: Arsenic trioxide (As2O3) has shown effectiveness in treatment of leukemia but is also associated with reproductive toxicity. Since remediation with N-acetylcysteine (NAC) may mitigate the adverse effects caused by exposure, we assessed the effects of As2O3 and its potential reversibility after exposure cessation or coadministration of NAC. Animals received 0.3 or 3.0 mg/Kg/day of As2O3 subcutaneously and 40 mM of NAC in tap water. As2O3 treatment impaired spermatogenesis and sperm motility and decreased seminal vesicle weight and testosterone serum levels; after suspension of treatment, these parameters remained altered. When NAC was administered, animals showed improvement in sperm parameters and seminal vesicle weight. In vitro epididymal contractility was increased in As2O3-treated animals. We concluded that As2O3 is toxic to the male mouse genital system by compromising sperm quality and quantity; these effects persisted even after suspension of the treatment. However, the coadministration of NAC ameliorates the harmful effects of the drug on the male genital system.
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Raquel Frenedoso
authors.initials: RF
authors.lastname: da Silva
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Cibele dos Santos
authors.initials: Cdos S
authors.lastname: Borges
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Villela E Silva
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Gabriela
authors.identifier: 0000-0001-8686-144X
authors.initials: G
authors.lastname: Missassi
authors.affiliation: Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Luiz Ricardo Almeida
authors.initials: LR
authors.lastname: Kiguti
authors.affiliation: Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: André Sampaio
authors.initials: AS
authors.lastname: Pupo
authors.affiliation: Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences, University of São Paulo (USP), 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Barbosa Junior
authors.affiliation: Department of Morphology, Stomatology, and Physiology, School of Dentistry, University of São Paulo (USP), 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Janete Aparecida
authors.initials: JA
authors.lastname: Anselmo-Franci
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Wilma De Grava
authors.initials: Wde G
authors.lastname: Kempinas
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage*; D000818:Animals; D001151:Arsenic / Q000097:blood; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage* / Q000009:adverse effects; D001835:Body Weight; D004822:Epididymis / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D009929:Organ Size; D010087:Oxides / Q000008:administration & dosage* / Q000009:adverse effects; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000502:physiology; D013739:Testosterone / Q000097:blood / Q000378:metabolism*
chemical_list: D001152:Arsenicals; D010087:Oxides; D017382:Reactive Oxygen Species; D013739:Testosterone; D001151:Arsenic; D000077237:Arsenic Trioxide; D000111:Acetylcysteine
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