title: [Effect of AAVC-I on the migration of human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To investigate the effect of component I from agkistrodon acutus venomon (AAVC-I) the migration of human umbilical vein endothelial cells (HUVECs), and to elucidate the possible anti-angiogenic mechanism of AAVC-I.
  
  
  METHODS
  The effect of AAVC-I on the migration of HUVECs which was cultivated in vitro and treated with AAVC-1 at four concentrations: 0, 20, 40, 80 microg/ml, was observed by methods of scratch wound-healing and Transwell assay. The expression level of mRNA and protein of P-selectin and intercellular cell adhension molecule-I (ICAM-1) were examined by RT-PCR and Western blot assay.
  
  
  RESULTS
  Compared with the blank group, the migration ability of HUVECs in each AAVE-I treated group was reduced in a dose-dependent manner, and the expression level of the mRNA and protein of P-selectin and ICAM-1 were decreased.
  
  
  CONCLUSIONS
  AAVC-I inhibits the migration of endothelial cell, which is acted by down-regulation of the expression content of mRNA and protein of P-selectin and ICAM-1.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Long
authors.forename: Jin-li
authors.initials: JL
authors.lastname: Jia
authors.forename: Gen-bao
authors.initials: GB
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Hong
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D003435:Crotalid Venoms / Q000494:pharmacology*; D015536:Down-Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D012333:RNA, Messenger
chemical_list: C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D019007:P-Selectin; D012333:RNA, Messenger; C546937:SELP protein, human; D018799:Intercellular Adhesion Molecule-1