title: MicroRNA-26b suppresses the metastasis of non-small cell lung cancer by targeting MIEN1 via NF-κB/MMP-9/VEGF pathways.
abstract: MicroRNAs (miRNAs) are involved in tumor initiation and progression. MiR-26b was reported to be significantly downregulated in non-small cell lung cancer (NSCLC). However, the underlying mechanisms of miR-26b involvement in the development and progression of NSCLC remains poorly understood. In the present study, we report that miR-26b suppresses cell metastasis in NSCLC through targeting migration and invasion enhancer 1 (MIEN1). We found that miR-26b was significantly downregulated and MIEN1 was significantly upregulated in both NSCLC tissues and cells lines. The expression levels of miR-26b were negatively related to those of MIEN1 mRNA in clinical NSCLC tissues. Furthermore, MIEN1 was confirmed to be a direct target of miR-26b by dual-luciferase reporter assay and MIEN1 expression was downregulated by miR-26b in NSCLC cells. In terms of function, transwell and wound healing assays demonstrated that the miR-26b remarkably inhibited invasion and migration of NSCLC cells, which was simulated by siRNA knockdown of MIEN1 and reversed by pcDNA/MIEN1 overexpression of MIEN1. Finally, we found that miR-26b could regulate NF-κB/MMP-9/VEGF pathway in NSCLC cells. In conclusion, this study revealed that miR-26b suppresses NSCLC metastasis by targeting MIEN1 via NF-κB/MMP-9/VEGF pathways, implicating a potential prognostic biomarker and therapeutic target for NSCLC treatment.
authors.affiliation: Department of Pharmaceutical, Affiliated Hospital of Qingdao University, 16 Jiangsu Rd, Qingdao, Shandong, 266003, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, 16 Jiangsu Rd, Qingdao, Shandong, 266003, PR China. Electronic address: wycheng3456@163.com.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, 16 Jiangsu Rd, Qingdao, Shandong, 266003, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, 16 Jiangsu Rd, Qingdao, Shandong, 266003, PR China.
authors.forename: Huijiang
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, 16 Jiangsu Rd, Qingdao, Shandong, 266003, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Liu
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology / Q000556:secondary*; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D020780:Matrix Metalloproteinase 9; D035683:MicroRNAs; D016328:NF-kappa B; D009363:Neoplasm Proteins; D042461:Vascular Endothelial Growth Factor A; C517054:MIEN1 protein, human; C528163:MIRN26 microRNA, human; C467484:VEGFA protein, human; C579270:MMP9 protein, human
keywords: MIEN1; Metastasis; MiR-26b; NF-κB/MMP-9/VEGF pathways; NSCLC