title: Improved osteoblasts growth on osteomimetic hydroxyapatite/BaTiO3 composites with aligned lamellar porous structure.
abstract: Osteoblasts growing into bone substitute is an important step of bone regeneration. This study prepared porous hydroxyapatite (HA)/BaTiO3 piezoelectric composites with porosity of 40%, 50% and 60% by ice-templating method. Effects of HA/BaTiO3 composites with different porosities, with and without polarizing treatment on adhesion, proliferation and differentiation of osteoblasts were investigated in vitro. Results revealed that cell densities of the porous groups were significantly higher than those of the dense group (p<0.05), so did the alkaline phosphate (ALP) and bone gla protein (BGP) activities. Porosity of 50% group exhibited higher ALP activity and BGP activity than those of the 40% and 60% groups. Scanning electron microscopy (SEM) observations revealed that osteoblasts adhered and stretched better on porous HA/BaTiO3 than on the dense one, especially HA/BaTiO3 with porosity of 50% and 60%. However, there was no significant difference in the cell morphology, cell densities, ALP and BGP activities between the polarized group and the non-polarized group (p>0.05). The absence of mechanical loading on the polarized samples may account for this. The results indicated that hierarchically porous HA/BaTiO3 played a favorable part in osteoblasts proliferation, differentiation and adhesion process and is a promising bone substitute material.
authors.affiliation: Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Beilei
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China; State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083, PR China. Electronic address: jian007040@sina.com.
authors.forename: Liangjian
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Kechao
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083, PR China. Electronic address: dzhang@csu.edu.cn.
authors.forename: Dou
authors.initials: D
authors.lastname: Zhang
mesh_terms: D017609:Barium Compounds / Q000737:chemistry / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D017609:Barium Compounds; D018786:Bone Substitutes; D017886:Durapatite
keywords: Biological piezoelectric ceramic; Hydroxyapatite/BaTiO(3); Osteoblast; Polarization; Porosity