title: A novel conductometric biosensor based on hexokinase for determination of adenosine triphosphate.
abstract: The paper presents a simple and inexpensive reusable biosensor for determination of the concentration of adenosine-5'-triphosphate (ATP) in aqueous samples. The biosensor is based on a conductometric transducer which contains two pairs of gold interdigitated electrodes. An enzyme hexokinase was immobilized onto one pair of electrodes, and bovine serum albumin-onto another pair (thus, a differential mode of measurement was used). Conditions of hexokinase immobilization on the transducer by cross-linking via glutaraldehyde were optimized. Influence of experimental conditions (concentration of magnesium ions, ionic strength and concentration of the working buffer) on the biosensor work was studied. The reproducibility of biosensor responses and operational stability of the biosensor were checked during one week. Dry storage at -18 °C was shown to be the best conditions to store the biosensor. The biosensor was successfully applied for measurements of ATP concentration in pharmaceutical samples. The proposed biosensor may be used in future for determination of ATP and/or glucose in water samples.
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotny Str., Kyiv 03680, Ukraine; Taras Shevchenko National University of Kyiv, 64 Volodymyrska str., 01003 Kyiv, Ukraine; Institute of Analytical Sciences, UMR5280 CNRS/UCBL/ENS, 5 rue de la Doua, 69100, Villeurbanne, France. Electronic address: kucherenko.i.s@gmail.com.
authors.forename: I S
authors.initials: IS
authors.lastname: Kucherenko
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotny Str., Kyiv 03680, Ukraine; Taras Shevchenko National University of Kyiv, 64 Volodymyrska str., 01003 Kyiv, Ukraine.
authors.forename: D Yu
authors.initials: DY
authors.lastname: Kucherenko
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotny Str., Kyiv 03680, Ukraine; Taras Shevchenko National University of Kyiv, 64 Volodymyrska str., 01003 Kyiv, Ukraine.
authors.forename: O O
authors.initials: OO
authors.lastname: Soldatkin
authors.affiliation: Institute of Analytical Sciences, UMR5280 CNRS/UCBL/ENS, 5 rue de la Doua, 69100, Villeurbanne, France.
authors.forename: F
authors.initials: F
authors.lastname: Lagarde
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotny Str., Kyiv 03680, Ukraine; Taras Shevchenko National University of Kyiv, 64 Volodymyrska str., 01003 Kyiv, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Dzyadevych
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotny Str., Kyiv 03680, Ukraine; Taras Shevchenko National University of Kyiv, 64 Volodymyrska str., 01003 Kyiv, Ukraine.
authors.forename: A P
authors.initials: AP
authors.lastname: Soldatkin
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002021:Buffers; D003217:Conductometry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006593:Hexokinase / Q000737:chemistry* / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014159:Transducers
chemical_list: D002021:Buffers; D004800:Enzymes, Immobilized; D006046:Gold; D000255:Adenosine Triphosphate; D006593:Hexokinase; D008274:Magnesium; D005976:Glutaral
keywords: Adenosine triphosphate; Conductometric biosensor; Glucose; Hexokinase; Immobilized enzymes