title: Sorption of testosterone on partially-dispersed soil particles of different size fractions: Methodology and implications.
abstract: Sorption of hormones to soil particles of different size fractions (DSFs) has been studied to understand their fate and transport (F/T) in soils. Conventional studies fractionated the soil particles into DSFs by using the high speed stirring method and/or adding surfactants to fully disperse the bulk soil. However, the natural processes (e.g., soil erosion, irrigation) often are relatively mild, and many soil particles may be still in the aggregate form. In this study, a method was developed for conducting the sorption test of a representative hormone (i.e., testosterone) to bulk soils first and then analyzing the results against DSFs. Results indicated the particle size distribution (PSD) of the two representative soils tested with partially-dispersed and fully-dispersed methods was significantly different due to the attachment of clay particles on sand and silt. Testosterone was sorbed mainly by the dominant aggregates even though they might have relatively lower sorption affinity than that of clays. However, the small particles (<2000 nm), even with ∼5% mass of the bulk soil, contributed more than 30% of sorbed testosterone in the "whole" soils. The partially-dispersed soil particles of DSFs should be used to understand the transport of hormone in runoff, because using the fully-dispersed soil particles will overestimate while the whole soil method will underestimate the transport potential. With the methodology developed in this study, the sorption tests will not compromise soil's original properties (e.g., aggregates) or the competition (e.g., sorption) among soil particles, and the contribution of DSFs (particularly the partially-dispersed aggregates) to the sorption of the "whole" soil can be determined.
authors.affiliation: 136 PKI, Civil Engineering Dept., University of Nebraska-Lincoln, Omaha, NE 68182, USA. Electronic address: yqi01@unomaha.edu.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qi
authors.affiliation: 205D PKI, Civil Engineering Dept., University of Nebraska-Lincoln, Omaha, NE 68182, USA.
authors.forename: Tian C
authors.initials: TC
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D002244:Carbon / Q000032:analysis; D052918:Environmental Restoration and Remediation / Q000379:methods*; D008970:Molecular Weight; D009930:Organic Chemicals / Q000032:analysis; D010316:Particle Size; D012987:Soil / Q000737:chemistry*; D013739:Testosterone / Q000302:isolation & purification*
chemical_list: D009930:Organic Chemicals; D012987:Soil; D013739:Testosterone; D002244:Carbon
keywords: Partially-dispersed; Particle size fraction; Soil; Sorption; Testosterone