title: Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia.
abstract: Interactions between GluR2 and N-ethylmaleimide-sensitive factor (NSF) mediate AMPA receptors trafficking. This might be linked with molecular mechanisms related with memory formation. Previous research has shown basolateral amygdala (BLA) dependent activity changes in the perirhinal cortex (PRh) during the formation of taste memory. In the present experiments we investigate both the behavioral performance and the expression profile of NSF and GluR2 genes in several brain areas, including PRh, BLA, and hippocampus. Twenty-one naïve male Wistar rats were exposed to a saccharin solution (0.4%) during the first (novel), the second (Familiar I), and the sixth presentation (Familiar II). Total RNA was extracted and gene expression was measured by quantitative PCR (qPCR) using TaqMan gene expression assays. In addition the expression of the synaptic plasticity related immediate early genes, Homer 1 and Narp, was also assessed. We have found increased expression of NSF gene in BLA and PRh in Group Familiar I in comparison with Familiar II. No changes in the expression of GluR2, Homer 1, and Narp genes were found. The results suggest the relevance of a potential network in the temporal lobe for taste recognition memory and open new possibilities for understanding the molecular mechanisms mediating the impact of sensory experience on brain circuit function.
authors.affiliation: Department of Psychobiology, Center for Biomedical Research (CIBM), Institute of Neurosciences, University of Granada, Avenida del Conocimiento, s/n, 18071 Granada, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gómez-Chacón
authors.affiliation: Department of Psychobiology, Center for Biomedical Research (CIBM), Institute of Neurosciences, University of Granada, Avenida del Conocimiento, s/n, 18071 Granada, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gámiz
authors.affiliation: Department of Neuroscience, McKnight Brain Institute, University of Florida, P.O. Box 100244, Gainesville, FL 32610-0244, USA.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Foster
authors.affiliation: Department of Psychobiology, Center for Biomedical Research (CIBM), Institute of Neurosciences, University of Granada, Avenida del Conocimiento, s/n, 18071 Granada, Spain.
authors.forename: Milagros
authors.identifier: 0000-0002-9712-6106
authors.initials: M
authors.lastname: Gallo
mesh_terms: D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D006185:Habituation, Psychophysiologic / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D000072233:Homer Scaffolding Proteins; D008297:Male; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism; D013649:Taste / Q000502:physiology*; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; D009419:Nerve Tissue Proteins; D018091:Receptors, AMPA; D000099153:Pentraxins; C498737:Nsf protein, rat; C104722:glutamate receptor ionotropic, AMPA 2
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