title: Effects of 10-hydroxycamptothecin on intrinsic mitochondrial pathway in silkworm BmN-SWU1 cells.
abstract: 10-Hydroxycamptothecin (HCPT), a plant alkaloid isolated from Camptotheca acuminate, is known as a planted-derived insecticide, however, the specific mechanism in insect cells is still unclear. In this study, we treated the ovarian cell line of the silkworm, BmN-SWU1, with different HCPT doses for durations ranging from 0 to 72h. The apoptosis morphology was evident after 72h of incubation and included cell protuberance, concentrated cytoplasm and apoptotic bodies. We observed DNA fragmentation and cell apoptosis after HCPT treatment. The disruption of mitochondrial distribution, activation of the intracellular mitochondrial permeability transition pore, and release of cytochrome c during HCPT-induced apoptosis in dose and time-dependent manner indicate the involvement of mitochondria in BmN-SWU1 cells. Caspase-9 and -3 activities increased gradually with the duration of incubation time. In conclusion, HCPT has a significant effect to initiate the intrinsic mitochondrial pathway in silkworm cells, providing a theoretical basis for better application of plant-derived insecticide in pest control.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Guiyang College of Traditional Chinese Medicine, Guiyang City, Guizhou Province 550025, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
authors.forename: Yan-Fen
authors.initials: YF
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Yi
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
authors.forename: La
authors.initials: L
authors.lastname: Wang
authors.affiliation: Guiyang College of Traditional Chinese Medicine, Guiyang City, Guizhou Province 550025, China.
authors.forename: Yi-Ying
authors.initials: YY
authors.lastname: Yang
authors.affiliation: Guiyang College of Traditional Chinese Medicine, Guiyang City, Guizhou Province 550025, China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Wang
authors.affiliation: Guiyang College of Traditional Chinese Medicine, Guiyang City, Guizhou Province 550025, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address: pmh0476@hotmail.com.
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address: lucheng@swu.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis; D012831:Bombyx; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D005260:Female; D008928:Mitochondria / Q000187:drug effects*; D010053:Ovary / Q000166:cytology
chemical_list: C028098:10-hydroxycamptothecin; D002166:Camptothecin
keywords: Apoptosis; BmN-SWU1 cell line; Bombyx mori; HCPT; Mitochondria