title: Overlapping signatures of chronic pain in the DNA methylation landscape of prefrontal cortex and peripheral T cells.
abstract: We tested the hypothesis that epigenetic mechanisms in the brain and the immune system are associated with chronic pain. Genome-wide DNA methylation assessed in 9 months post nerve-injury (SNI) and Sham rats, in the prefrontal cortex (PFC) as well as in T cells revealed a vast difference in the DNA methylation landscape in the brain between the groups and a remarkable overlap (72%) between differentially methylated probes in T cells and prefrontal cortex. DNA methylation states in the PFC showed robust correlation with pain score of animals in several genes involved in pain. Finally, only 11 differentially methylated probes in T cells were sufficient to distinguish SNI or Sham individual rats. This study supports the plausibility of DNA methylation involvement in chronic pain and demonstrates the potential feasibility of DNA methylation markers in T cells as noninvasive biomarkers of chronic pain susceptibility.
authors.affiliation: Faculty of Medicine, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Renaud
authors.initials: R
authors.lastname: Massart
authors.affiliation: Faculty of Medicine, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Dymov
authors.affiliation: Faculty of Dentistry, Alan Edwards Centre for Research on Pain, McGill University, Montreal, Quebec, Canada.
authors.forename: Magali
authors.initials: M
authors.lastname: Millecamps
authors.affiliation: Faculty of Medicine, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Matthew
authors.initials: M
authors.lastname: Suderman
authors.affiliation: Faculty of Dentistry, Alan Edwards Centre for Research on Pain, McGill University, Montreal, Quebec, Canada.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Gregoire
authors.affiliation: Faculty of Medicine, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Kevin
authors.initials: K
authors.lastname: Koenigs
authors.affiliation: Faculty of Medicine, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Alvarado
authors.affiliation: Faculty of Dentistry, Alan Edwards Centre for Research on Pain, McGill University, Montreal, Quebec, Canada.
authors.forename: Maral
authors.initials: M
authors.lastname: Tajerian
authors.affiliation: Faculty of Dentistry, Alan Edwards Centre for Research on Pain, McGill University, Montreal, Quebec, Canada.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Stone
authors.affiliation: Faculty of Medicine, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Moshe
authors.initials: M
authors.lastname: Szyf
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D059350:Chronic Pain / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019175:DNA Methylation; D008297:Male; D017397:Prefrontal Cortex / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers
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