title: [Rosiglitazone protects against endotoxin-induced acute liver injury in rats].
abstract: OBJECTIVE
  To observe the effects of rosiglitazone (ROSI) on endotoxin-induced acute liver injury in rats.
  
  
  METHODS
  All the animals were randomly assigned to one of four following groups (n=24 per group). Rats in the Sepsis-Dimethyl sulfoxide (DMSO) group(n=24), rats were treated with 10%DMSO(1 ml/kg) 30 mins before they were challenged with cecal ligation and puncture(CLP) (n=24). In the sepsis-rosiglitazone (ROSI) group was identical to the sepsis-DMSO group but received ROSI (10 mg/kg intravenously) (n=24). In the sham-DMSO group, rats were treated with 10% DMSO(1 ml/kg) 30 mins before the administration of saline (n=24). The sham-ROSI group was identical to the sham-ROSI group except that ROSI (10 mg/kg intravenously) was administered instead of DMSO (n=24). Sepsis was induced in rats by dissecting the cecum and placing the discontinued organ back into the abdomen CLP. And all the Rats were decapitated at 0, 6, 12, 24 h after the model completed.Serum glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase (GPT), interleukin 12 (IL-12) were determined to assess activity and liver pathologic change of rats in different groups. Data were analyzed with SPSS 19.0 software.Student's t-test was used to compare densitometry data.
  
  
  RESULTS
  SPSS 19.0 statistical software analysis by the outcome of the application ELASA method for determining zero hours between every group, there was non-difference. The sepsis-ROSI group and the sepsis-DMSO group 12 hours and 24 hours the presence of plasma GOT concentration changes statistically significant difference (t12=3.97, P12=0.01; t24=3.10, P24<0.001), (t12=0.55, P12<0.001; t24=4.32, P24<0.001). The Sepsis-ROSI group and the sepsis-DMSO group 6 hours, 12 hours and 24 hours the presence of plasma GPT concentration changes statistically significant difference (t6=3.17, P6=0.021; t12=2.81, P12=0.018; t24=5.06, P24<0.001), (t6=2.67, P6=0.001; t12=3.36, P12=0.002; t24=2.83, P24<0.001). The sepsis-ROSI group and the sepsis-DMSO group 6 hours, 12 hours and 24 hours the presence of plasma IL-12 concentration changes statistically significant difference(t6=3.17, P6=0.021; t12=2.81, P12=0.018; t24=5.06, P24<0.001), (t6=2.67, P6=0.001; t12=3.36, P12=0.002; t24=2.83, P24<0.001). Sepsis GOT, GPT, IL-12 began to increase in 6 hours, 24 hours of high-value, no clear peak trend, the sepsis-ROSI group compared with the sham-ROSI group had lower concentrations of GOT, GPT and IL-12 to some extent. Description rosiglitazone inhibit inflammatory cytokines in rats with sepsis role of IL-12, there is a certain role to reduce the inflammatory response in sepsis.
  
  
  CONCLUSIONS
  Treatment with Thiazolidinedione, markedly inhibited the expression of IL-12, attenuated pulmonary inflammation and liver injury caused by sepsis in rat.
authors.affiliation: Intensive Care Unit, the Second Hospital of Dalian Medical University, Dalian 116027, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Bu
authors.affiliation: Intensive Care Unit, the Second Hospital of Dalian Medical University, Dalian 116027, China; Email: yujian@medmail.com.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
mesh_terms: D000410:Alanine Transaminase; D000818:Animals; D001219:Aspartate Aminotransferases; D056486:Chemical and Drug Induced Liver Injury; D016207:Cytokines; D004731:Endotoxins; D008099:Liver; D051381:Rats; D000077154:Rosiglitazone; D018805:Sepsis; D045162:Thiazolidinediones
chemical_list: D016207:Cytokines; D004731:Endotoxins; D045162:Thiazolidinediones; D000077154:Rosiglitazone; C089946:2,4-thiazolidinedione; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase