title: A duck reovirus variant with a unique deletion in the sigma C gene exhibiting high pathogenicity in Pekin ducklings.
abstract: A novel strain of duck reovirus (DRV) associated with a high mortality in Pekin ducklings in China, 2013, was isolated and characterized. This strain (designated as HN5d) grew well in Vero cells and produced marked cytopathic effects. HN5d contains 10 dsRNA genome segments, a typical feature of avian orthoreovirus. Following cloning, sequencing, and sequence analysis of the genome segments, a unique deletion of 18 amino acids was found in the sigma C protein of HN5d when compared with that of the recent Chinese waterfowl reoviruses (e.g., DRV 091). Phylogenetic analysis of cDNA amplicons of segments encoding for the outer capsid proteins revealed that HN5d is a novel genotype 2 waterfowl reovirus isolate. Inoculation of Pekin ducklings with HN5d resulted in splenic necrosis, a typical feature of "Duck spleen necrosis disease" (DSND) discovered in China in 2006. Unlike the typical DSND, HN5d produced severer hemorrhagic and/or necrotic lesions in livers of experimentally infected ducklings. 20-30% of death was observed during the first 7 day in the experimentally exposed birds. These findings suggest that HN5d is a novel duck reovirus isolate with severer pathogenicity in Pekin ducklings.
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Ning
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Te
authors.initials: T
authors.lastname: Liang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Minghang
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China. Electronic address: zdb@cau.edu.cn.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000473:pathology* / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004372:Ducks; D005838:Genotype; D009336:Necrosis / Q000473:pathology; D030101:Orthoreovirus, Avian / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D010802:Phylogeny; D012088:Reoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D013154:Spleen / Q000473:pathology; D014709:Vero Cells; D014776:Virus Cultivation
chemical_list: D036022:Capsid Proteins; C031728:sigma 1 protein, reovirus
keywords: Duck reovirus; Genotype 2; Pekin Duck; Sigma C gene deletion; Waterfowl reovirus