title: Determination of synthetic phenolic antioxidants in edible oils using microvial insert large volume injection gas-chromatography.
abstract: Three synthetic phenolic antioxidants, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butyl hydroquinone (TBHQ), were determined in different edible vegetable oil samples. The analyses were carried out by gas chromatography-mass spectrometry (GC-MS) using microvial insert large volume injection (LVI). Several parameters affecting this sample introduction step, such as temperatures, times and gas flows, were optimised. Quantification was carried out by the matrix-matched calibration method using carvacrol as internal standard, providing quantification limits between 0.08 and 0.10 ng g(-1), depending on the compound. The three phenolic compounds were detected in several of the samples, BHT being the most frequently found. Recovery assays for oil samples spiked at two concentration levels, 2.5 and 10 ng g(-1), provided recoveries in the 86-115% range.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100 Murcia, Spain.
authors.forename: Juan Ignacio
authors.initials: JI
authors.lastname: Cacho
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100 Murcia, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Campillo
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100 Murcia, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Viñas
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100 Murcia, Spain. Electronic address: hcordoba@um.es.
authors.forename: Manuel
authors.initials: M
authors.lastname: Hernández-Córdoba
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002084:Butylated Hydroxytoluene / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006873:Hydroquinones / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*
chemical_list: D000975:Antioxidants; D006873:Hydroquinones; D010938:Plant Oils; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C018855:2-tert-butylhydroquinone
keywords: Butylated hydroxyanisole (BHA); Butylated hydroxytoluene (BHT); Edible oil samples; Gas chromatography–mass spectrometry (GC–MS); Synthetic phenolic antioxidants; Tert-butyl hydroquinone (TBHQ)