title: Polymerase recognition of 2-thio-iso-guanine·5-methyl-4-pyrimidinone (iGs·P)--A new DD/AA base pair.
abstract: Polymerase specificity is reported for a previously unknown base pair with a non-standard DD/AA hydrogen bonding pattern: 2-thio-iso-guanine·5-methyl-4-pyrimidinone. Our findings suggest that atomic substitution may provide a solution for low fidelity previously associated with enzymatic copying of iso-guanine.
authors.affiliation: Department of Chemistry, University of California, Riverside, CA 92521, United States.
authors.forename: Dong-Kye
authors.initials: DK
authors.lastname: Lee
authors.affiliation: Department of Chemistry, University of California, Riverside, CA 92521, United States. Electronic address: switzer@ucr.edu.
authors.forename: Christopher
authors.initials: C
authors.lastname: Switzer
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006860:Hydrogen Bonding; D009841:Oligonucleotides / Q000096:biosynthesis / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011744:Pyrimidinones / Q000737:chemistry / Q000378:metabolism*; D044366:Transition Temperature
chemical_list: C000611251:2-thio-iso-guanine 5-methyl-4-pyrimidinone; D009841:Oligonucleotides; D011744:Pyrimidinones; D006147:Guanine
keywords: Base-pair; Nucleic acids; Origins of life; Synthetic biology