title: Assessment of productivity of Culex spp. larvae (Diptera: Culicidae) in urban storm water catch basin system in Wrocław (SW Poland).
abstract: In urban environments, catch basins serve as major developmental and resting sites for anthropophilic and zoophilic mosquitoes. However, the use of this habitat is inconsistent, with abundance of larvae varying significantly across catch basins at a fine spatial scale. During seasonal summer investigations on mosquito species composition, their spatial and temporal distribution and the environmental characteristic of the breeding sites in the underground storm drain systems of the Wrocław urban area (SW Poland) were assessed from May to September in 2012-2013. The study was conducted in order to develop a rational strategy to control mosquito populations and prevent the potential human exposure to mosquito-transmitted pathogens. Mosquito larvae and pupae were collected and identified weekly from 100 regularly inspected street catch basins located in the town center. All existing and potential breeding habitats in the study area were recorded using a GPS receiver (Magellan MobileMapper CX) and transferred to the computer database. Collected data on the geographical location of inspected breeding places, water quality parameters in inspected catch basins, daily temperature, and precipitation were imposed on orthophotomap in ArcGIS (ESRI, USA). Water quality parameters including pH, electrical conductivity, and water temperature were measured by standard methods. Chemical water analysis of cations (Na(+), NH4 (+), K(+), Mg(2+), Ca(2+)) and anions (Cl(-), NO2 (-), NO3 (-), SO4 (2-)) were carried out using Waters Alliance high-performance liquid chromatograph (HPLC) 2695 with 432 Conductivity Detector and 2998 Photodiode Array Detector, an IC-Pak Anion HR column (glauconate/borate eluent) and IC-Pak Cation M/D column (EDTA/HNO3 eluent). Over two seasonal studies and 3739 samplings in total, 3669 mosquito larvae and 274 pupae/1 dip (from 0 to 110 individuals/dip) were collected by dipper. Culex pipiens s.l. (L.) and Cx. torrentium (Martini) prevailed at all catch basins of the study area as the predominant species. In all examined catch basins, autogenous individuals dominated by far. Breeding activity was first detected in early May. Peak abundance of Culex spp. population in many catch basins was observed in June 2012 and August 2013 when average daily temperatures were increasing and rainfall had declined. Dry periods between rainfalls varied during 2 years of the study period and were noted on June 2012 as well as on July and August 2013. Organically enriched catch basins with significant higher concentrations of Na(+) and NO3 (-) were found to be more productive breeding habitats. Differences in the Culex immature stage density based on the variables of habitat type, temperature, and precipitation support the need for ongoing surveillance in communities to guide public health officials in planning for and prioritizing mosquito control efforts.
authors.affiliation: Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wrocław, 63/77 Przybyszewskiego Street, 51-148, Wrocław, Poland. katarzyna.rydzanicz@uwr.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rydzanicz
authors.affiliation: Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wrocław, 63/77 Przybyszewskiego Street, 51-148, Wrocław, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Jawień
authors.affiliation: Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wrocław, 63/77 Przybyszewskiego Street, 51-148, Wrocław, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Lonc
authors.affiliation: Institute of Geological Sciences, Environmental Geology Laboratory, University of Wrocław, Max Born Square 9, 50-205, Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Modelska
mesh_terms: D000818:Animals; D002947:Cities; D003465:Culex / Q000502:physiology*; D004323:Drainage, Sanitary; D004777:Environment; D007814:Larva / Q000502:physiology; D009032:Mosquito Control / Q000379:methods; D011044:Poland; D011679:Pupa; D012621:Seasons; D013696:Temperature; D014867:Water
chemical_list: D014867:Water
keywords: Catch basins; Culex pipiens s.l; Cx. torrentium; Mosquitoes in Poland; Seasonal and spatial distribution of larvae
references.citation: Emerg Infect Dis. 1999 Sep-Oct;5(5):643-50
references.pmid: 10511520
references.citation: Euro Surveill. 2014 Apr 24;19(16):20779
references.pmid: 24786260
references.citation: Emerg Infect Dis. 2008 Jan;14(1):41-7
references.pmid: 18258075
references.citation: Euro Surveill. 2011 Aug 04;16(31):
references.pmid: 21871217
references.citation: J Am Mosq Control Assoc. 2012 Dec;28(4 Suppl):53-67
references.pmid: 23401944
references.citation: Am J Trop Med Hyg. 2011 Sep;85(3):551-3
references.pmid: 21896821
references.citation: Clin Microbiol Infect. 2013 Aug;19(8):685-92
references.pmid: 23574618
references.citation: J Med Entomol. 2008 Jan;45(1):28-35
references.pmid: 18283939
references.citation: Euro Surveill. 2011 Aug 04;16(31):
references.pmid: 21871214
references.citation: J Med Entomol. 2012 Mar;49(2):270-6
references.pmid: 22493843
references.citation: Heredity (Edinb). 1999 Jan;82 ( Pt 1):7-15
references.pmid: 10200079
references.citation: J Med Entomol. 2015 Sep;52(5):932-6
references.pmid: 26336210
references.citation: Parasitol Res. 2015 Jan;114(1):289-95
references.pmid: 25339516
references.citation: J Am Mosq Control Assoc. 2010 Jun;26(2):172-82
references.pmid: 20649127
references.citation: J Am Mosq Control Assoc. 2008 Mar;24(1):54-60
references.pmid: 18437815
references.citation: J Med Entomol. 2006 Sep;43(5):1010-9
references.pmid: 17017241
references.citation: Euro Surveill. 2012 Sep 06;17(36):20265
references.pmid: 22971331
references.citation: Clin Med Res. 2005 Feb;3(1):3-12
references.pmid: 15962015
references.citation: Vector Borne Zoonotic Dis. 2011 Oct;11(10):1383-9
references.pmid: 21923273
references.citation: J Am Mosq Control Assoc. 2011 Mar;27(1):45-55
references.pmid: 21476447
references.citation: J Med Entomol. 2010 Jan;47(1):43-52
references.pmid: 20180307
references.citation: J Vector Ecol. 2014 Jun;39(1):138-45
references.pmid: 24820566
references.citation: J Am Mosq Control Assoc. 1988 Sep;4(3):339-44
references.pmid: 3199124
references.citation: Parasit Vectors. 2013 Jan 11;6:9
references.pmid: 23311394
references.citation: Med Vet Entomol. 2014 Jun;28(2):179-86
references.pmid: 23947434
references.citation: Emerg Infect Dis. 2001 Jul-Aug;7(4):722-5
references.pmid: 11589172
references.citation: J Am Mosq Control Assoc. 1989 Dec;5(4):537-40
references.pmid: 2614404
references.citation: J Vector Ecol. 2003 Jun;28(1):79-89
references.pmid: 12831132
references.citation: J Am Mosq Control Assoc. 2009 Jun;25(2):140-8
references.pmid: 19653495
references.citation: Vector Borne Zoonotic Dis. 2012 Jun;12(6):435-47
references.pmid: 22448724
references.citation: Parasitol Res. 2008 Dec;103 Suppl 1:S19-28
references.pmid: 19030883
references.citation: J Vector Ecol. 2003 Dec;28(2):255-66
references.pmid: 14714675
references.citation: Am J Trop Med Hyg. 1993 Jul;49(1):30-7
references.pmid: 8352389