title: [Hemoperfusion alleviated paraquat-induced kidney inflammation injury of rabbit].
abstract: OBJECTIVE
  To investigate the effect of hemoperfusion on paraquat-Induced kidney inflammation injury of rabbit and the mechanism of it.
  
  
  METHODS
  60 male rabbits were randomly divided into 4 groups, the normal control group (n=6, the rabbits were given NS by gavage) , blank control group (n=18, he rabbits were given 2 hours hemoperfusion once within 1 hour after given NS by gavage), paraquat poisoning group (n=18, the rabbits were given 50 mg/kg 20% paraquat solution by gavage) , hemoperfusion treatment group (n=18, the rabbits were given 2 hours hemoperfusion once within 1 hour after 20% paraquat solution espoused). The last 3 groups were divided into 3 observation time groups (1, 3, 7 day), contained 6 rabbits each group. On days 1, 3, 7 all groups rabbits were anesthetized and sacrificed, and their kidney tissues collected. The levels of NF-κB mRNA by RT-PCR, and the expression of NF-κB protein was measured by Western blotting,The expression levels of TNF-α, IL-6, iNOS measured by chemical colorimetric method to to observe inflammatory injury.
  
  
  RESULTS
  Compared with the normal control group rabbits, there were no changes in the TNF-α, IL-6, iNOS, NF-κB mRNA and protein of blank control group (P>0.05), while the expression of TNF-α, IL-6, NF-κB mRNA and protein in the kidney tissue of PQ group and were significantly increased (P<0.05). The pathological results of kidney tissues were no abnormalities onnormal control group and blank control group.
  
  
  CONCLUSIONS
  HP significantly increase resistance to PQ-induced inflammation injury in the rabbit kidney and exert a protective effect on PQ-induced kidney injury.
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Shaoce
authors.initials: S
authors.lastname: Zhi
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lingwen
authors.initials: L
authors.lastname: Li
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Guangliang
authors.initials: G
authors.lastname: Hong
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Mengfang
authors.initials: M
authors.lastname: Li
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Emergency Department of the first Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; E-mail: lzq640815@163.com.
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000818:Animals; D006464:Hemoperfusion; D007249:Inflammation / Q000139:chemically induced / Q000628:therapy*; D015850:Interleukin-6 / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010269:Paraquat / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D010269:Paraquat