title: A calixpyridinium-based supramolecular tandem assay for alkaline phosphatase and its application to ATP hydrolysis reaction.
abstract: We have successfully implemented the supramolecular tandem assay principle for the real-time, continuous, direct, and label-free monitoring of alkaline phosphatase activity through a fluorescence "switch-off" assay based on a novel calixpyridinium/dye reporter pair. Because several diseases can be preliminarily diagnosed in light of an abnormal level of alkaline phosphatase in serum, the application of tandem assays to selectively monitor alkaline phosphatase activity has feasible implications in disease diagnosis.
authors.affiliation: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China. hxxywk@mail.tjnu.edu.cn hxxyxsy@mail.tjnu.edu.cn.
authors.forename: Kui
authors.initials: K
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China. hxxywk@mail.tjnu.edu.cn hxxyxsy@mail.tjnu.edu.cn.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Cui
authors.affiliation: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China. hxxywk@mail.tjnu.edu.cn hxxyxsy@mail.tjnu.edu.cn.
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Xing
authors.affiliation: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China. hxxywk@mail.tjnu.edu.cn hxxyxsy@mail.tjnu.edu.cn.
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Dou
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011725:Pyridines; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase