title: Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells.
abstract: BACKGROUND
  In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB).
  
  
  METHODS
  Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot.
  
  
  RESULTS
  Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences.
  
  
  CONCLUSIONS
  These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.
authors.affiliation: MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India. omanach@yahoo.com.|, Klusweg 18, 8032, Zurich, Switzerland. omanach@yahoo.com.
authors.forename: O A
authors.initials: OA
authors.lastname: Trentz
authors.affiliation: MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India.
authors.forename: D
authors.initials: D
authors.lastname: Arikketh
authors.affiliation: MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India.
authors.forename: V
authors.initials: V
authors.lastname: Sentilnathan
authors.affiliation: Institute of Clinical Research, University Hospital Zurich, Zurich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Hemmi
authors.affiliation: Institute of Clinical Research, University Hospital Zurich, Zurich, Switzerland.
authors.forename: A E
authors.initials: AE
authors.lastname: Handschin
authors.affiliation: MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India.
authors.forename: B
authors.initials: B
authors.lastname: de Rosario
authors.affiliation: MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India.
authors.forename: P
authors.initials: P
authors.lastname: Mohandas
authors.affiliation: MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India.
authors.forename: P V A
authors.initials: PV
authors.lastname: Mohandas
keywords: Adipose tissue-derived osteogenic cells; Osteoblast markers; Tissue engineering
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