title: The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast.
abstract: Saccharomyces cerevisiae Rch1 is structurally similar to both the vertebrate solute carrier SLC10A7 and Candida albicans Rch1. We show here that ScRCH1 is a functional homolog of CaRCH1. In S. cerevisiae, overexpression of ScRCH1 suppresses, but deletion of ScRCH1 does not affect, the lithium and rapamycin tolerance of pmr1 cells. Overexpression of ScRCH1 reduces expression of ENA1, prevents sustained accumulation of cytosolic calcium and reduces the activation level of calcium/calcineurin signaling in pmr1 cells. Therefore, similar to the situation in the pathogen C. albicans, ScRch1 negatively regulates the cytosolic homeostasis in response to high levels of extracellular calcium. ScRch1 proteins distribute as multiple foci in the plasma membrane prior to cell division, move toward and concentrate at the bud neck as the bud grows in size, and disperse again along the plasma membrane immediately prior to cytokinesis. Furthermore, our genetic and biochemical data also demonstrate that transcriptional expression of RCH1 is positively regulated by calcium/calcineurin signaling through the sole CDRE element in its promoter.
authors.affiliation: The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yunying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yan
authors.affiliation: Plant Nutrition Laboratory, Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin-Luther-University Halle-Wittenberg, 06099 Halle (Saale), Germany.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Happeck
authors.affiliation: Plant Nutrition Laboratory, Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin-Luther-University Halle-Wittenberg, 06099 Halle (Saale), Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Peiter-Volk
authors.affiliation: The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Xu
authors.affiliation: The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Plant Nutrition Laboratory, Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin-Luther-University Halle-Wittenberg, 06099 Halle (Saale), Germany.
authors.forename: Edgar
authors.initials: E
authors.lastname: Peiter
authors.affiliation: Centre for Microscopy and Cell Imaging, Concordia University, Montreal, Quebec H4B 1R6, Canada.
authors.forename: Chloë
authors.initials: C
authors.lastname: van Oostende Triplet
authors.affiliation: Department of Biology, Concordia University, Montreal, Quebec H4B 1R6, Canada.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Whiteway
authors.affiliation: The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: linghuojiang@jiangnan.edu.cn.
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002176:Candida albicans / Q000737:chemistry / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D006706:Homeostasis; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction; D028901:alpha Karyopherins / Q000378:metabolism*
chemical_list: D028901:alpha Karyopherins; C087978:karyopherin alpha 2; D019703:Calcineurin; D002118:Calcium
keywords: Bud neck; Calcium; Crz1; ENA1; PMR1; Plasma membrane; RCH1