title: Use of platelet-rich plasma in deep second- and third-degree burns.
abstract: Unfortunately burns are a common occurrence, leading to scarring or death. Platelet-rich plasma (PRP) contains many growth factors that can accelerate wound healing. We analyzed the use of PRP in deep second-degree (dSD), deep second-degree associated with diabetes mellitus (dSDD), and third-degree (TD) burns in rats. Sixty syngeneic rats divided into three groups (dSD, dSDD, and TD) were burned, half receiving topical PRP and half being used as control; 10 additional rats per group were used for PRP preparation. On day 21, the animals were sacrificed and skin biopsies were collected. dSD and dSDD wounds treated with PRP showed faster wound closure, reduction in CD31-, CD68-, CD163-, MPO-, and in TGF-β-positive cells, and an increase in MMP2-positive cells. The neo-epidermis was thinner in the control of both the dSD and dSDD groups and granulation tissue was less reduced in the control of both the dSDD and TD groups. These results indicate that PRP can accelerate the healing process in dSD and dSDD, but not in TD burns.
authors.affiliation: Department of General Surgery, Post-graduation Program in Physiopathology and Surgical Sciences-Rio de Janeiro State University, Brazil.
authors.forename: Neil Grant
authors.initials: NG
authors.lastname: Venter
authors.affiliation: Department of General Surgery, Post-graduation Program in Physiopathology and Surgical Sciences-Rio de Janeiro State University, Brazil.
authors.forename: Ruy Garcia
authors.initials: RG
authors.lastname: Marques
authors.affiliation: Department of Histology and Embryology, Rio de Janeiro State University, Brazil.
authors.forename: Jeanine Salles Dos
authors.initials: JS
authors.lastname: Santos
authors.affiliation: Department of Histology and Embryology, Rio de Janeiro State University, Brazil. Electronic address: amacosta@uerj.br.
authors.forename: Andréa
authors.initials: A
authors.lastname: Monte-Alto-Costa
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002056:Burns / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002921:Cicatrix / Q000473:pathology; D003094:Collagen / Q000032:analysis; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D004195:Disease Models, Animal; D006097:Granulation Tissue / Q000473:pathology; D008297:Male; D053657:Platelet-Rich Plasma; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; D016212:Transforming Growth Factor beta; D003094:Collagen
keywords: Burn; Healing; Platelet-rich plasma; Rat