title: MIIP expression predicts outcomes of surgically resected esophageal squamous cell carcinomas.
abstract: The migration and invasion inhibitory protein (MIIP) was shown to function as a tumor suppressor gene in gliomas by inhibiting tumor cell growth, migration, and invasion. However, its role and clinical significance in esophageal squamous cell carcinoma (ESCC) have not been elucidated. We investigated the correlation of MIIP expression and clinical outcome in a group of surgically resected ESCCs. Tissue microarrays constructed of 253 surgically resected ESCC primary tumors and paired paracancerous normal esophageal epithelia were used for MIIP evaluation by immunohistochemistry. The clinical and prognostic significance of MIIP expression was analyzed statistically. The expression of MIIP expression in cancer tissues was increased significantly in comparison with the paired paracancerous normal epithelia (P < 0.001). And, MIIP expression was associated with ESCC cells' differentiation (P < 0.001). By Kaplan-Meier analysis, patients with low MIIP expression exhibited significantly improved overall survival (OS, P = 0.039) and a tendency of improved disease-free survival (DFS, P = 0.086) than those with high MIIP expression. In addition, MIIP expression could distinguish OS or DFS of patients with tumors in stage T3-4 (P = 0.020, 0.028), N0 (P = 0.008, 0.032), and stage II (P = 0.004, 0.019), as well as at lower thoracic esophagus (P = 0.024, 0.090). Multivariate analysis showed that MIIP expression was an independent prognostic factor in ESCC OS and DFS. In conclusion, MIIP expressed higher in ESCCs than in paracancerous normal esophageal epithelia and was a positive, independent prognostic factor in resected ESCCs.
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.|Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.
authors.forename: Qian-Wen
authors.initials: QW
authors.lastname: Liu
authors.affiliation: Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.
authors.forename: Kong-Jia
authors.initials: KJ
authors.lastname: Luo
authors.affiliation: Department of Pathology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.|Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Xie
authors.affiliation: Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China. huyi@sysucc.org.cn.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China. huyi@sysucc.org.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China. fu_jianhua@hotmail.com.|Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China. fu_jianhua@hotmail.com.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China. fu_jianhua@hotmail.com.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Fu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D002352:Carrier Proteins / Q000032:analysis / Q000502:physiology*; D002454:Cell Differentiation; D018572:Disease-Free Survival; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D016629:Esophagectomy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D047908:Intracellular Signaling Peptides and Proteins; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000502:physiology*; D009367:Neoplasm Staging; D016037:Single-Blind Method; D046888:Tissue Array Analysis; D016896:Treatment Outcome
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C480900:MIIP protein, human; D009363:Neoplasm Proteins
keywords: Esophageal cancer; MIIP; Outcomes; Protein expression
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