title: Heme Oxygenase-1 Delays Gibberellin-Induced Programmed Cell Death of Rice Aleurone Layers Subjected to Drought Stress by Interacting with Nitric Oxide.
abstract: Cereal aleurone layers undergo a gibberellin (GA)-regulated process of programmed cell death (PCD) following germination. Heme oxygenase-1 (HO-1) is known as a rate-liming enzyme in the degradation of heme to biliverdin IXα, carbon monoxide (CO), and free iron ions (Fe(2+)). It is a critical component in plant development and adaptation to environment stresses. Our previous studies confirmed that HO-1 inducer hematin (Ht) promotes the germination of rice seeds in drought (20% polyethylene glycol-6000, PEG) conditions, but the corresponding effects of HO-1 on the alleviation of germination-triggered PCD in GA-treated rice aleurone layers remain unknown. The present study has determined that GA co-treated with PEG results in lower HO-1 transcript levels and HO activity, which in turn results in the development of vacuoles in aleurone cells, followed by PCD. The pharmacology approach illustrated that up- or down-regulated HO-1 gene expression and HO activity delayed or accelerated GA-induced PCD. Furthermore, the application of the HO-1 inducer Ht and nitric oxide (NO) donor sodium nitroprusside (SNP) not only activated HO-1 gene expression, HO activity, and endogenous NO content, but also blocked GA-induced rapid vacuolation and accelerated aleurone layers PCD under drought stress. However, both HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) and NO scavenger 2-(4-carboxyphenyl0-4, 4,5,5-tetramethylimidazoline-l-oxyl-3-oxide potassium salt (cPTIO) reserved the effects of Ht and SNP on rice aleurone layer PCD under drought stress by down-regulating endogenous HO-1 and NO, respectively. The inducible effects of Ht and SNP on HO-1 gene expression, HO activity, and NO content were blocked by cPTIO. Together, these results clearly suggest that HO-1 is involved in the alleviation of GA-induced PCD of drought-triggered rice aleurone layers by associating with NO.
authors.affiliation: College of Horticulture and Landscape Architecture, Hainan University Haikou, China.
authors.forename: Huangming
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Horticulture and Landscape Architecture, Hainan University Haikou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Horticulture and Landscape Architecture, Hainan University Haikou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Horticulture and Landscape Architecture, Hainan University Haikou, China.
authors.forename: Heting
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Horticulture and Landscape Architecture, Hainan University Haikou, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Chen
keywords: Oryza sativa; aleurone layers; drought stress; gibberellin; heme oxygenase-1; nitric oxide; programmed cell death
references.citation: Mol Biol Cell. 2009 Jan;20(1):21-32
references.pmid: 18923146
references.citation: Plant Mol Biol. 2000 Oct;44(3):255-66
references.pmid: 11199387
references.citation: Free Radic Biol Med. 2012 Aug 15;53(4):710-20
references.pmid: 22683602
references.citation: Annu Rev Pharmacol Toxicol. 2006;46:411-49
references.pmid: 16402911
references.citation: Ecotoxicol Environ Saf. 2014 Oct;108:114-9
references.pmid: 25046853
references.citation: J Exp Bot. 2012 Sep;63(15):5521-34
references.pmid: 22915740
references.citation: Planta. 2006 Oct;224(5):1154-62
references.pmid: 16703357
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16093-8
references.pmid: 12456881
references.citation: J Mol Med (Berl). 2008 Mar;86(3):267-79
references.pmid: 18034222
references.citation: Plant Cell. 1999 Jun;11(6):1033-46
references.pmid: 10368175
references.citation: J Immunol. 2006 Apr 1;176(7):4252-7
references.pmid: 16547262
references.citation: Plant Physiol. 2001 May;126(1):156-66
references.pmid: 11351079
references.citation: Plant J. 2011 Apr;66(2):280-92
references.pmid: 21205037
references.citation: Biochem Biophys Res Commun. 2004 Oct 22;323(3):1003-8
references.pmid: 15381099
references.citation: J Plant Physiol. 2012 Jul 15;169(11):1032-9
references.pmid: 22579358
references.citation: J Mol Cell Cardiol. 2010 Aug;49(2):186-95
references.pmid: 20430037
references.citation: Planta. 1986 Mar;169(1):51-63
references.pmid: 24232429
references.citation: Mol Cell Biochem. 2002 May-Jun;234-235(1-2):249-63
references.pmid: 12162441
references.citation: PLoS One. 2013 Dec 12;8(12 ):e81470
references.pmid: 24349074
references.citation: Antioxid Redox Signal. 2002 Apr;4(2):259-70
references.pmid: 12006177
references.citation: J Biol Chem. 2004 Mar 19;279(12):11530-6
references.pmid: 14699134
references.citation: Plant Physiol. 2002 Dec;130(4):1958-66
references.pmid: 12481078
references.citation: Nature. 1998 Aug 6;394(6693):585-8
references.pmid: 9707120
references.citation: Curr Opin Plant Biol. 2006 Oct;9(5):523-9
references.pmid: 16860591
references.citation: Plant Physiol. 2002 Jul;129(3):954-6
references.pmid: 12114551
references.citation: J Exp Bot. 2011 Jan;62(1):235-48
references.pmid: 20797999
references.citation: FEBS J. 2006 Dec;273(23 ):5384-99
references.pmid: 17076701
references.citation: Int J Mol Sci. 2014 Nov 14;15(11):21155-78
references.pmid: 25405743
references.citation: J Exp Bot. 2010 May;61(9):2255-70
references.pmid: 20378668
references.citation: Plant Physiol. 2002 Aug;129(4):1642-50
references.pmid: 12177477
references.citation: Plant Cell. 1996 Feb;8(2):259-69
references.pmid: 8742711
references.citation: New Phytol. 2008;177(1):155-66
references.pmid: 18028301
references.citation: Plant Physiol. 2008 Oct;148(2):881-93
references.pmid: 18689445
references.citation: J Biol Chem. 2000 May 19;275(20):15166-73
references.pmid: 10809751
references.citation: J Exp Bot. 2011 Aug;62(13):4675-89
references.pmid: 21673093
references.citation: J Plant Physiol. 2010 Nov 1;167(16):1371-9
references.pmid: 20674075
references.citation: J Exp Bot. 2012 Jun;63(10 ):3869-83
references.pmid: 22419743
references.citation: Int J Mol Sci. 2011;12 (4):2488-501
references.pmid: 21731454
references.citation: Phytochemistry. 2010 Oct;71(14-15):1700-7
references.pmid: 20708206
references.citation: Planta. 2007 Oct;226(5):1155-63
references.pmid: 17569079
references.citation: J Plant Res. 2011 May;124(3):315-24
references.pmid: 21409543
references.citation: Int Rev Cell Mol Biol. 2008;270:87-144
references.pmid: 19081535
references.citation: Plant Mol Biol. 1996 Dec;32(6):1125-34
references.pmid: 9002611
references.citation: Oncogene. 2004 Apr 12;23(16):2881-90
references.pmid: 15077151
references.citation: Plant Cell Rep. 2012 Sep;31(9):1563-72
references.pmid: 22532008
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Antioxid Redox Signal. 2002 Aug;4(4):603-14
references.pmid: 12230872
references.citation: Physiol Rev. 2006 Apr;86(2):583-650
references.pmid: 16601269
references.citation: Plant Cell Rep. 2015 Mar;34(3):381-93
references.pmid: 25433859
references.citation: Plant J. 2001 Jan;25(1):19-29
references.pmid: 11169179
references.citation: Cell Death Differ. 2011 Aug;18(8):1298-304
references.pmid: 21637288
references.citation: Plant Cell Environ. 2008 Dec;31(12 ):1864-81
references.pmid: 18811735