title: The efficacy of tyrosine kinase inhibitor dasatinib on colonic mucosal damage in murine model of colitis.
abstract: OBJECTIVE
  Ulcerative colitis is an inflammatory condition of the colon in the gastrointestinal system. Currently, the most potent medications used for ulcerative colitis produce no response in 20-30% of cases. There is a need for more efficient and reliable medications. Tyrosine kinase inhibitors have shown efficacy in some inflammatory diseases. Although dasatinib, a tyrosine kinase inhibitor, suppresses proinflammatory cytokines in colonic tissue, there are a few cases of hemorrhagic colitis with dasatinib. There is no study investigating the effect of dasatinib on experimental colitis. We aimed to investigate the effect of dasatinib in a colitis model induced with acetic acid in our study.
  
  
  METHODS
  In the study, 24 male Sprague-Dawley rats randomly distributed into 4 groups of 6 rats each as control, dasatinib, colitis and dasatinib+colitis groups. For colitis induction, 4% acetic acid was used. Sacrificing of the rats was performed on the seventh day. Disease activity, morphologic and histological injury, superoxide dismutase, myeloperoxidase and malondialdehyde activity, TNFα and CD3 expression were assessed in colonic tissue.
  
  
  RESULTS
  Apart from malondialdehyde, significant difference in all parameters between the control and colitis groups was determined. Difference between the colitis and colitis+dasatinib groups was not significant in only weight loss and biochemical parameters. Though dasatinib does not fully resolve the changes in colitis, there was significant regression.
  
  
  CONCLUSIONS
  Dasatinib decreased the inflammation in a rodent model of colitis. It may be provide this effect by the suppression of TNFα. Dasatinib may be one of the treatment options for ulcerative colitis.
authors.affiliation: Department of Gastroenterology, Abant İzzet Baysal University, Faculty of Medicine, Gölköy, 14280 Bolu, Turkey. Electronic address: dr_guraycan@yahoo.com.
authors.forename: Güray
authors.initials: G
authors.lastname: Can
authors.affiliation: Department of Pediatric Surgery, Trakya University, Faculty of Medicine, Edirne, Turkey. Electronic address: suleyayvaz@yahoo.com.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Ayvaz
authors.affiliation: Department of Internal Medicine, Abant İzzet Baysal University, Faculty of Medicine, Bolu, Turkey. Electronic address: yailaslan@yahoo.com.
authors.forename: Hatice
authors.initials: H
authors.lastname: Can
authors.affiliation: Department of Histology and Embryology, Trakya University, Faculty of Medicine, Edirne, Turkey. Electronic address: ihsankaraboga@gmail.com.
authors.forename: İhsan
authors.initials: İ
authors.lastname: Karaboğa
authors.affiliation: Department of Histology and Embryology, Trakya University, Faculty of Medicine, Edirne, Turkey. Electronic address: selimdemirtas@trakya.edu.tr.
authors.forename: Selim
authors.initials: S
authors.lastname: Demirtaş
authors.affiliation: Department of Biochemistry, Balıkesir University, Faculty of Veterinary, Balıkesir, Turkey. Electronic address: vethas@hotmail.com.
authors.forename: Hasan
authors.initials: H
authors.lastname: Akşit
authors.affiliation: Department of Gastroenterology, Selçuk University, Faculty of Medicine, Konya, Turkey. Electronic address: dr.yilmazbulent@gmail.com.
authors.forename: Bülent
authors.initials: B
authors.lastname: Yılmaz
authors.affiliation: Department of Gastroenterology, Abant İzzet Baysal University, Faculty of Medicine, Gölköy, 14280 Bolu, Turkey. Electronic address: drkorkmazugur@yahoo.com.
authors.forename: Uğur
authors.initials: U
authors.lastname: Korkmaz
authors.affiliation: Department of Gastroenterology, Abant İzzet Baysal University, Faculty of Medicine, Gölköy, 14280 Bolu, Turkey. Electronic address: dr.mevlutkurt@gmail.com.
authors.forename: Mevlüt
authors.initials: M
authors.lastname: Kurt
authors.affiliation: Department of Histology and Embryology, Trakya University, Faculty of Medicine, Edirne, Turkey. Electronic address: turankaraca@trakya.edu.tr.
authors.forename: Turan
authors.initials: T
authors.lastname: Karaca
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology; D000069439:Dasatinib / Q000494:pharmacology*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D015431:Weight Loss
chemical_list: D047428:Protein Kinase Inhibitors; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D000069439:Dasatinib