title: Early Ankle Mobilization Promotes Healing in a Rabbit Model of Achilles Tendon Rupture.
abstract: The use of early mobilization of the ankle joint without orthosis in the treatment of Achilles tendon rupture has been advocated as the optimal management. The goal of this study was to compare outcomes in a postoperative rabbit model of Achilles tendon rupture between early mobilization and immobilized animals using a differential proteomics approach. In total, 135 rabbits were randomized into the control group (n=15), the postoperative cast immobilization (PCI) group (n=60), and the early mobilization (EM) group (n=60). A rupture of the Achilles tendon was created in each animal model and repaired microsurgically, and tendon samples were removed at 3, 7, 14, and 21 days postoperatively. Proteins were separated using 2-dimensional polyacrylamide gel electrophoresis and identified using peptide mass fingerprinting, tandem mass spectrometry, NCBI database searches, and bioinformatics analyses. A series of differentially expressed proteins were identified between groups, some of which may play an important role in Achilles tendon healing. Notable candidate proteins that were upregulated in the EM group were identified, such as CRMP-2, galactokinase 1, tropomyosin-4, and transthyretin. The healing of ruptured Achilles tendons appears to be affected at the level of protein expression with the use of early mobilization. The classic postoperative treatment of Achilles tendon rupture with an orthosis ignored the self-protecting instinct of humans. With a novel operative technique, the repaired tendon can persist the load that comes from traction in knee and ankle joint functional movement. In addition, kinesitherapy provided an excellent experimental outcome via a mechanobiological mechanism.
authors.forename: Jiasharete
authors.initials: J
authors.lastname: Jielile
authors.forename: Batiza
authors.initials: B
authors.lastname: Asilehan
authors.forename: Aikeremu
authors.initials: A
authors.lastname: Wupuer
authors.forename: Bayixiati
authors.initials: B
authors.lastname: Qianman
authors.forename: Ayidaer
authors.initials: A
authors.lastname: Jialihasi
authors.forename: Wulanbai
authors.initials: W
authors.lastname: Tangkejie
authors.forename: Abudouheilil
authors.initials: A
authors.lastname: Maimaitiaili
authors.forename: Nuerai
authors.initials: N
authors.lastname: Shawutali
authors.forename: Aynaz
authors.initials: A
authors.lastname: Badelhan
authors.forename: Hadelebieke
authors.initials: H
authors.lastname: Niyazebieke
authors.forename: Adili
authors.initials: A
authors.lastname: Aizezi
authors.forename: Amuding
authors.initials: A
authors.lastname: Aisaiding
authors.forename: Yerzat
authors.initials: Y
authors.lastname: Bakyt
authors.forename: Rakimbaiev
authors.initials: R
authors.lastname: Aibek
authors.forename: Jianati
authors.initials: J
authors.lastname: Wuerliebieke
mesh_terms: D000125:Achilles Tendon / Q000293:injuries* / Q000378:metabolism / Q000601:surgery*; D000818:Animals; D002370:Casts, Surgical; D004434:Early Ambulation; D005686:Galactokinase / Q000378:metabolism; D007103:Immobilization; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D023421:Models, Animal; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011182:Postoperative Care; D011228:Prealbumin / Q000378:metabolism; D011817:Rabbits; D012421:Rupture / Q000601:surgery; D014335:Tropomyosin / Q000378:metabolism; D015854:Up-Regulation; D014945:Wound Healing
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011228:Prealbumin; C501213:TPM4 protein, human; D014335:Tropomyosin; C095114:collapsin response mediator protein-2; C484715:GALK1 protein, human; D005686:Galactokinase