title: Zinc supplementation ameliorates glycoprotein components and oxidative stress changes in the lung of streptozotocin diabetic rats.
abstract: Zinc (Zn) is a component of numerous enzymes that function in a wide range of biological process, including growth, development, immunity and intermediary metabolism. Zn may play a role in chronic states such as cardiovascular disease and diabetes mellitus. Zn acts as cofactor and for many enzymes and proteins and has antioxidant, antiinflammatory and antiapoptotic effects. Taking into consideration that lung is a possible target organ for diabetic complications, the aim of this study was to investigate the protective role of zinc on the glycoprotein content and antioxidant enzyme activities of streptozotocin (STZ) induced diabetic rat tissues. Female Swiss albino rats were divided into four groups. Group I, control; Group II, control + zinc sulfate; Group III, STZ-diabetic; Group IV, diabetic + zinc sulfate. Diabetes was induced by intraperitoneal injection of STZ (65 mg/kg body weight). Zinc sulfate was given daily by gavage at a dose of 100 mg/kg body weight every day for 60 days to groups II and IV. At the last day of the experiment, rats were sacrificed, lung tissues were taken. Also, glycoprotein components, tissue factor (TF) activity, protein carbonyl (PC), advanced oxidative protein products (AOPP), hydroxyproline, and enzyme activities in lung tissues were determined. Glycoprotein components, TF activity, lipid peroxidation, non enzymatic glycation, PC, AOPP, hydroxyl proline, lactate dehydrogenase, catalase, superoxide dismutase, myeloperoxidase, xanthine oxidase, adenosine deaminase and prolidase significantly increased in lung tissues of diabetic rats. Also, glutathione levels, paraoxonase, arylesterase, carbonic anhydrase, and Na(+)/K(+)- ATPase activities were decreased. Administration of zinc significantly reversed these effects. Thus, the study indicates that zinc possesses a significantly beneficial effect on the glycoprotein components and oxidant/antioxidant enzyme activities.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar/Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Sacan
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar/Istanbul, Turkey.
authors.forename: Ismet Burcu
authors.initials: IB
authors.lastname: Turkyilmaz
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar/Istanbul, Turkey.
authors.forename: Bertan Boran
authors.initials: BB
authors.lastname: Bayrak
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar/Istanbul, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Mutlu
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, 34116, Istanbul, Turkey.
authors.forename: Nuriye
authors.initials: N
authors.lastname: Akev
authors.affiliation: Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar/Istanbul, Turkey. refiyeyanardag@yahoo.com.
authors.forename: Refiye
authors.initials: R
authors.lastname: Yanardag
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D019587:Dietary Supplements; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000378:metabolism; D019287:Zinc Sulfate / Q000008:administration & dosage*
chemical_list: D013311:Streptozocin; D019287:Zinc Sulfate; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D043303:Aryldialkylphosphatase; D002256:Carbonic Anhydrases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Diabetes mellitus; Glycoprotein; Lung; Oxidative stress; Zinc