title: The role of soluble and insoluble gastric fluid components in the pathogenesis of obliterative bronchiolitis in rat lung allografts.
abstract: Repetitive gastric fluid aspirations have been shown to lead to obliterans bronchiolitis (OB), but the component or components of gastric fluid that are responsible are unknown. This study investigates the role of particulates and, separately, soluble material in gastric fluid during the development of OB. Whole gastric fluid (WGF) was collected from male Fischer 344 (F344) rats and separated by centrifugation into particle reduced gastric fluid (PRGF) and particulate components resuspended in normal saline (PNS). Orthotopic left lung transplants from male Wistar-Kyoto rats into F344 rats were performed using a modification of the nonsuture external cuff technique with prolonged cold ischemia. Rats were subjected to weekly aspiration of 0.5 ml/kg of WGF (n = 9), PRGF (n = 10), PNS (n = 9), or normal saline (control, NS; n = 9) for 8 weeks following transplantation. Lung allografts treated with WGF, PRGF, or PNS developed a significantly greater percentage of OB-like lesions compared with the control. No statistical difference was observed when comparing the fibrosis grades or the percentage of OB lesions of WGF, PRGF, and PNS groups, suggesting that both soluble and insoluble components of gastric fluid can promote the development of aspiration-induced OB and fibrosis in lung allografts.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.|Department of Pathology, Duke University Medical Center, Durham, NC, USA.
authors.forename: Jason H
authors.initials: JH
authors.lastname: Leung
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.|Department of Pathology, Duke University Medical Center, Durham, NC, USA.|Division of Thoracic and Cardiovascular Surgery, Hualien Tzu Chi Hospital, Hualien, Taiwan.|Department of Surgery, Tzu Chi University, Hualien, Taiwan.
authors.forename: Jui-Chih
authors.initials: JC
authors.lastname: Chang
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: Sadé M
authors.initials: SM
authors.lastname: Bell
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: Zoie E
authors.initials: ZE
authors.lastname: Holzknecht
authors.affiliation: Department of Biostatistics & Bioinformatics, Duke University Medical Center, Durham, NC, USA.
authors.forename: Samantha M
authors.initials: SM
authors.lastname: Thomas
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: Mary Lou
authors.initials: ML
authors.lastname: Everett
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: William
authors.initials: W
authors.lastname: Parker
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: R Duane
authors.initials: RD
authors.lastname: Davis
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.|Department of Pathology, Duke University Medical Center, Durham, NC, USA.|Department of Immunology, Duke University Medical Center, Durham, NC, USA.
authors.forename: Shu S
authors.initials: SS
authors.lastname: Lin
mesh_terms: D000818:Animals; D001989:Bronchiolitis Obliterans / Q000209:etiology*; D005753:Gastric Mucosa / Q000378:metabolism*; D008168:Lung / Q000473:pathology; D008170:Lung Compliance; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D011658:Pulmonary Fibrosis / Q000209:etiology; D051381:Rats; D011916:Rats, Inbred F344; D011921:Rats, Inbred WKY; D053120:Respiratory Aspiration / Q000150:complications*; D014184:Transplantation, Homologous
keywords: aspiration; bronchiolitis obliterans syndrome; chronic lung allograft damage; pulmonary transplantation; rat