title: Fluorescence Lifetime Imaging of Nanoflares for mRNA Detection in Living Cells.
abstract: The expression level of tumor-related mRNA can reveal significant information about tumor progression and prognosis, so specific mRNA in cells provides an important approach for biological and disease studies. Here, fluorescence lifetime imaging of nanoflares in living cells was first employed to detect specific intracellular mRNA. We characterized the lifetime changes of the prepared nanoflares before and after the treatment of target mRNA and also compared the results with those of fluorescence intensity-based measurements both intracellularly and extracellularly. The nanoflares released the cy5-modified oligonucleotides and bound to the targets, resulting in a fluorescence lifetime lengthening. This work puts forward another dimension of detecting specific mRNA in cells and can also open new ways for detection of many other biomolecules.
authors.affiliation: State Key Laboratory of Tribology, Tsinghua University , Beijing 100084, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Tribology, Tsinghua University , Beijing 100084, People's Republic of China.|Department of Industrial Engineering, Purdue University , 225 South University Street, West Lafayette, Indiana 47907, United States.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: School of Medicine, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Tribology, Tsinghua University , Beijing 100084, People's Republic of China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Tribology, Tsinghua University , Beijing 100084, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Industrial Engineering, Purdue University , 225 South University Street, West Lafayette, Indiana 47907, United States.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Tribology, Tsinghua University , Beijing 100084, People's Republic of China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Yang
authors.affiliation: School of Medicine, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
authors.forename: Yaocheng
authors.initials: Y
authors.lastname: Sun
mesh_terms: D019313:BRCA1 Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism
chemical_list: D019313:BRCA1 Protein; D002232:Carbocyanines; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C085321:cyanine dye 5