title: Effects of Melatonin on Oxidative Stress Index and Alveolar Bone Loss in Diabetic Rats With Periodontitis.
abstract: The aim of this study is to evaluate the effects of systemic melatonin treatment on serum oxidative stress index (OSI) and alveolar bone loss (ABL) in rats with diabetes mellitus (DM) and periodontitis.
  
  
  
  Seventy Sprague Dawley rats were divided into control, experimentally induced periodontitis (EP), DM, EP-DM, EP and melatonin treatment (EP-MEL), DM and melatonin treatment (DMMEL), and EP-DM-MEL groups. DM was induced by alloxan, after which periodontitis was induced by ligature for 4 weeks. After removal of the ligature, the rats in the melatonin groups (EP-MEL, DM-MEL, and EP-DM-MEL) were treated with a single dose of melatonin (10 mg/body weight) every day for 14 consecutive days. At the end of the study, all of the rats were euthanized, and intracardiac blood samples and mandible tissues were obtained for biochemical and histologic analyses. Serum levels of total oxidant status/total antioxidant status and OSI were measured. In addition, neutrophil and osteoclast densities and myeloperoxidase activities were determined in gingival tissue homogenates, and ABL was evaluated with histometric measurements.
  
  
  
  Melatonin treatment significantly reduced fasting plasma glucose levels in the rats with DM. In addition, reduced OSI and ABL levels were detected in the EP-MEL and DM-MEL groups; the reductions in the EP-DM-MEL group were found to be more prominent. Melatonin also significantly decreased the increased myeloperoxidase activities and osteoclast and neutrophil densities in the EP, DM, and EP-DM groups.
  
  
  
  It is revealed in this experimental study that melatonin significantly inhibited hyperglycemia-induced oxidative stress and ABL through antiDM and antioxidant effects in rats with DM and periodontitis.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Oguz
authors.initials: O
authors.lastname: Kose
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Ataturk University, Erzurum, Turkey.
authors.forename: Taner
authors.initials: T
authors.lastname: Arabaci
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Ataturk University.
authors.forename: Adem
authors.initials: A
authors.lastname: Kara
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Yemenoglu
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Ataturk University, Erzurum, Turkey.
authors.forename: Eda
authors.initials: E
authors.lastname: Kermen
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Pamukkale University, Denizli, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Kizildag
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Ataturk University.
authors.forename: Semin
authors.initials: S
authors.lastname: Gedikli
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University.
authors.forename: Seckin
authors.initials: S
authors.lastname: Ozkanlar
mesh_terms: D016301:Alveolar Bone Loss / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants; D003921:Diabetes Mellitus, Experimental; D008550:Melatonin / Q000502:physiology*; D018384:Oxidative Stress; D010518:Periodontitis / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D008550:Melatonin
keywords: Anti-inflammatory agents; antioxidants; diabetes mellitus; melatonin; oxidative stress; periodontitis