title: Rad61/Wpl1 (Wapl), a cohesin regulator, controls chromosome compaction during meiosis.
abstract: Meiosis-specific cohesin, required for the linking of the sister chromatids, plays a critical role in various chromosomal events during meiotic prophase I, such as chromosome morphogenesis and dynamics, as well as recombination. Rad61/Wpl1 (Wapl in other organisms) negatively regulates cohesin functions. In this study, we show that meiotic chromosome axes are shortened in the budding yeast rad61/wpl1 mutant, suggesting that Rad61/Wpl1 negatively regulates chromosome axis compaction. Rad61/Wpl1 is required for efficient resolution of telomere clustering during meiosis I, indicating a positive effect of Rad61/Wpl1 on the cohesin function required for telomere dynamics. Additionally, we demonstrate distinct activities of Rad61/Wpl1 during the meiotic recombination, including its effects on the efficient processing of intermediates. Thus, Rad61/Wpl1 both positively and negatively regulates various cohesin-mediated chromosomal processes during meiosis.
authors.affiliation: Institute for Protein Research, Graduate School of Science, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kiran
authors.initials: K
authors.lastname: Challa
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Institute for Protein Research, Graduate School of Science, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Shinohara
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Keun P
authors.initials: KP
authors.lastname: Kim
authors.affiliation: Institute for Protein Research, Graduate School of Science, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan ashino@protein.osaka-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Shinohara
mesh_terms: D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D015825:Chromosomes, Fungal / Q000378:metabolism; D008540:Meiosis / Q000235:genetics*; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016615:Telomere
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C476199:RAD61 protein, S cerevisiae; C502898:REC8 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
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