title: Extensive Recombination of a Yeast Diploid Hybrid through Meiotic Reversion.
abstract: In somatic cells, recombination between the homologous chromosomes followed by equational segregation leads to loss of heterozygosity events (LOH), allowing the expression of recessive alleles and the production of novel allele combinations that are potentially beneficial upon Darwinian selection. However, inter-homolog recombination in somatic cells is rare, thus reducing potential genetic variation. Here, we explored the property of S. cerevisiae to enter the meiotic developmental program, induce meiotic Spo11-dependent double-strand breaks genome-wide and return to mitotic growth, a process known as Return To Growth (RTG). Whole genome sequencing of 36 RTG strains derived from the hybrid S288c/SK1 diploid strain demonstrates that the RTGs are bona fide diploids with mosaic recombined genome, derived from either parental origin. Individual RTG genome-wide genotypes are comprised of 5 to 87 homozygous regions due to the loss of heterozygous (LOH) events of various lengths, varying between a few nucleotides up to several hundred kilobases. Furthermore, we show that reiteration of the RTG process shows incremental increases of homozygosity. Phenotype/genotype analysis of the RTG strains for the auxotrophic and arsenate resistance traits validates the potential of this procedure of genome diversification to rapidly map complex traits loci (QTLs) in diploid strains without undergoing sexual reproduction.
authors.affiliation: Institut Curie, PSL Research University, CNRS, UMR 3244, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 3244, Paris, France.
authors.forename: Raphaëlle
authors.initials: R
authors.lastname: Laureau
authors.affiliation: Institut Curie, PSL Research University, CNRS, UMR 3244, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 3244, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Loeillet
authors.affiliation: Institute of Research on Cancer and Ageing of Nice (IRCAN), CNRS UMR 7284-INSERM U1081, Faculté de Médecine, Université de Nice Sophia Antipolis, Nice, France.
authors.forename: Francisco
authors.initials: F
authors.lastname: Salinas
authors.affiliation: Institute of Research on Cancer and Ageing of Nice (IRCAN), CNRS UMR 7284-INSERM U1081, Faculté de Médecine, Université de Nice Sophia Antipolis, Nice, France.
authors.forename: Anders
authors.initials: A
authors.lastname: Bergström
authors.affiliation: Institut Curie, PSL Research University, Next Generation Sequencing Platform, Paris, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Legoix-Né
authors.affiliation: Institute of Research on Cancer and Ageing of Nice (IRCAN), CNRS UMR 7284-INSERM U1081, Faculté de Médecine, Université de Nice Sophia Antipolis, Nice, France.
authors.forename: Gianni
authors.initials: G
authors.lastname: Liti
authors.affiliation: Institut Curie, PSL Research University, CNRS, UMR 3244, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 3244, Paris, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Nicolas
mesh_terms: D002469:Cell Separation; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D004171:Diploidy; D005785:Gene Conversion / Q000235:genetics; D014644:Genetic Variation; D016681:Genome, Fungal; D006239:Haplotypes / Q000235:genetics; D006720:Homozygote; D006824:Hybridization, Genetic; D008540:Meiosis / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*
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