title: Polymerization of modified diaspirin cross-linked hemoglobin (DCLHb) with 1,6-bismaleimic-hexane.
abstract: Increasing the size of hemoglobin (Hb) by polymerization offers the benefits of reduced renal clearance and increased duration in the vascular circulation. With this goal, diaspirin cross-linked hemoglobin (DCLHb) was modified in order to keep one thiol group on the surface and then polymerized with 1,6-bismaleimic-hexane (1,6-BMH) to increase the molecular weight. The HPLC results indicated that approximate 20% dimers to tetramers of DCLHb desired were achieved after the polymerization. It was also demonstrated that the oxygen-carrying capacity of the products was similar to natural heme. The present study is expected to improve the efficacy of the DCLHb as an oxygen therapeutic agent.
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Qi
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Guo
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
mesh_terms: D001241:Aspirin / Q000031:analogs & derivatives* / Q000737:chemistry; D001802:Blood Substitutes / Q000138:chemical synthesis* / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans
chemical_list: D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin
keywords: Cross-linking agent; DCLHb; oxygen affinity; thiol group