title: Expression of Nemo-like kinase was increased and negatively correlated with the expression of TCF4 in lung cancers.
abstract: Nemo-like kinase (NLK), as a mitogen activated protein kinase (MAPK)-like kinase, is involved in the development of several human cancers. In this study, we explored the expression of NLK in lung squamous cell carcinoma (SCC) and adenocarcinoma tissues, and investigated the associations among NLK, β-catenin, T-cell factor 4 (TCF4), and the clinicopathological factors of lung cancers. The expressions of NLK, β-catenin, TCF4 were examined in 109 cases of lung cancers using immunohistochemistry method. The expression of NLK was observed in the nuclei of lung cancer tissues, and was significantly higher in lung cancer tissues than that in corresponding normal lung tissues (t = 21.636, n = 109, P < 0.001). The high expression of NLK was found in 45 cases of lung SCCs (45/49, 91.84%), which was much more than that in adenocarcinomas (38/60, 63.33%) (P = 0.001). Furthermore, the high expression of NLK was negatively correlated with TCF4 expression and positively correlated with the membranous expression of β-catenin. In conclusion, the present study demonstrated that the expression of NLK was localized in nucleus and significantly increased in lung cancers. The expression of NLK was negatively correlated with TCF4 expression and positively correlated with β-catenin membranous expression in lung cancers.
authors.affiliation: Department of Pathology, The Fourth Affiliated Hospital of China Medical University Shenyang, China.
authors.forename: Xiu-Wei
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University Shenyang 110001, China.
authors.forename: Song-Yan
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Urinary Surgery, The Fourth Affiliated Hospital of China Medical University Shenyang, China.
authors.forename: Dong-Wei
authors.initials: DW
authors.lastname: Xue
authors.affiliation: Department of Pathology, The Fourth Affiliated Hospital of China Medical University Shenyang, China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Xu
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University Shenyang 110001, China.
authors.forename: Lian-He
authors.initials: LH
authors.lastname: Yang
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University Shenyang 110001, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Xu
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University Shenyang 110001, China.
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000096:biosynthesis*; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D014408:Biomarkers, Tumor; C495270:CTNNB1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin; C470551:NLK protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Nemo-like kinase; T-cell factor 4; lung neoplasm; wnt signaling pathway; β-catenin
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