title: Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.
abstract: Nanoparticle delivery is becoming an increasingly more valuable technique in cancer drug treatments. The use of fluorescent probes, in particular, can provide noninvasive strategies to interrogate the internalization mechanisms of cancer cells and aid in drug design. Here we describe the delivery of fluorescently labeled polysaccharide-based nanoparticles to breast cancer cells in vitro and their subsequent immunofluorescence microscopy examination. The description of the synthesis, preparation, and delivery of the nanoparticles can be widely applicable to other in vitro drug delivery studies.
authors.affiliation: Department of Materials Science and Engineering, Stony Brook University, Stony Brook, NY, 11794, USA.
authors.forename: Derek
authors.initials: D
authors.lastname: Rammelkamp
authors.affiliation: Department of Materials Science and Engineering, Stony Brook University, Stony Brook, NY, 11794, USA.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Li
authors.affiliation: Chemical and Molecular Engineering Program, Department of Materials Science and Engineering, Stony Brook University, 246 Heavy Engineering, Stony Brook, NY, 11794-2275, USA. yizhi.meng@stonybrook.edu.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Meng
mesh_terms: D000818:Animals; D001692:Biological Transport; D001943:Breast Neoplasms / Q000473:pathology*; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D015925:Cryopreservation; D005456:Fluorescent Dyes / Q000737:chemistry*; D042541:Intracellular Space / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D013242:Sterilization
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D011134:Polysaccharides; C089187:cyanine dye 3
keywords: Breast cancer cells; Delivery; Fluorescence microscopy; Nanoparticles