title: Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection.
abstract: Cruciferous plants are important edible vegetables widely consumed around the world, including cabbage, cauli-flower and broccoli. The main disease that affects crucifer plants is black rot, caused by Xanthomonas campestris pv. campestris (Xcc). In order to better understand this specific plant-pathogen interaction, proteins responsive to Xcc infection in resistant (União) and susceptible (Kenzan) Brassica oleracea cultivars were investigated by 2-DE followed by mass spectrometry. A total of 47 variable spots were identified and revealed that in the susceptible interaction there is a clear reduction in the abundance of proteins involved in energetic metabolism and defense. It was interesting to observe that in the resistant interaction, these proteins showed an opposite behavior. Based on our results, we conclude that resistance is correlated with the ability of the plant to keep sufficient photosynthesis metabolism activity to provide energy supplies necessary for an active defense. As a follow-up study, qRT-PCR analysis of selected genes was performed and revealed that most genes showed an up-regulation trend from 5 to 15days after inoculation (DAI), showing highest transcript levels at 15DAI. These results revealed the gradual accumulation of transcripts providing a more detailed view of the changes occurring during different stages of the plant-pathogen interaction.
  
  
  
  In this study we have compared cultivars of Brassica oleracea (cabbage), susceptible and resistant to black rot, by using the classical 2-DE approach. We have found that resistance is correlated with the ability of the plant to keep sufficient photosynthesis metabolism activity to provide energy supplies necessary for an active defense.
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
authors.forename: Gabriela R C
authors.initials: GRC
authors.lastname: Villeth
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
authors.forename: Lílian S T
authors.initials: LST
authors.lastname: Carmo
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
authors.forename: Luciano Paulino
authors.initials: LP
authors.lastname: Silva
authors.affiliation: Embrapa Hortaliças, Gama, DF, Brazil.
authors.forename: Mateus Figueiredo
authors.initials: MF
authors.lastname: Santos
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
authors.forename: Osmundo Brilhante
authors.initials: OB
authors.lastname: de Oliveira Neto
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
authors.forename: Maria Fátima
authors.initials: MF
authors.lastname: Grossi-de-Sá
authors.affiliation: União Pioneira da Integração Social, DF, Brazil; Embrapa Cerrados, Planaltina, DF, Brazil.
authors.forename: Igor Sousa
authors.initials: IS
authors.lastname: Ribeiro
authors.affiliation: Embrapa Cerrados, Planaltina, DF, Brazil.
authors.forename: Suelen Nogueira
authors.initials: SN
authors.lastname: Dessaune
authors.affiliation: Embrapa Cerrados, Planaltina, DF, Brazil.
authors.forename: Rodrigo Rocha
authors.initials: RR
authors.lastname: Fragoso
authors.affiliation: Centro de Análises Proteomicas e Bioquimicas, Programa de Pós-Graduação em Ciencias Genomicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil; S-Inova, Programa de Pos-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.
authors.forename: Octávio L
authors.initials: OL
authors.lastname: Franco
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil. Electronic address: angela.mehta@embrapa.br.
authors.forename: Angela
authors.initials: A
authors.lastname: Mehta
mesh_terms: D001937:Brassica / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D054884:Host-Pathogen Interactions / Q000276:immunology*; D013058:Mass Spectrometry; D010788:Photosynthesis; D040901:Proteomics / Q000379:methods; D015854:Up-Regulation; D016959:Xanthomonas campestris / Q000472:pathogenicity / Q000502:physiology*
keywords: Black rot; Plant–pathogen interaction; Proteome; qRT-PCR