title: Expression of terminal oxidases under nutrient-starved conditions in Shewanella oneidensis: detection of the A-type cytochrome c oxidase.
abstract: Shewanella species are facultative anaerobic bacteria that colonize redox-stratified habitats where O2 and nutrient concentrations fluctuate. The model species Shewanella oneidensis MR-1 possesses genes coding for three terminal oxidases that can perform O2 respiration: a bd-type quinol oxidase and cytochrome c oxidases of the cbb3-type and the A-type. Whereas the bd- and cbb3-type oxidases are routinely detected, evidence for the expression of the A-type enzyme has so far been lacking. Here, we investigated the effect of nutrient starvation on the expression of these terminal oxidases under different O2 tensions. Our results reveal that the bd-type oxidase plays a significant role under nutrient starvation in aerobic conditions. The expression of the cbb3-type oxidase is also modulated by the nutrient composition of the medium and increases especially under iron-deficiency in exponentially growing cells. Most importantly, under conditions of carbon depletion, high O2 and stationary-growth, we report for the first time the expression of the A-type oxidase in S. oneidensis, indicating that this terminal oxidase is not functionally lost. The physiological role of the A-type oxidase in energy conservation and in the adaptation of S. oneidensis to redox-stratified environments is discussed.
authors.affiliation: CNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, UMR 7281, IMM, 13402 Marseille Cedex 20, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Le Laz
authors.affiliation: CNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, UMR 7281, IMM, 13402 Marseille Cedex 20, France.
authors.forename: Arlette
authors.initials: A
authors.lastname: Kpebe
authors.affiliation: CNRS, Aix-Marseille Université, Unité de fermentation, FR3479, IMM, 13402 Marseille Cedex 20, France.
authors.forename: Marielle
authors.initials: M
authors.lastname: Bauzan
authors.affiliation: CNRS, Aix-Marseille Université, Plate-forme Protéomique, FR3479, IMM, MaP IBiSA, 13402 Marseille Cedex 20, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Lignon
authors.affiliation: CNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, UMR 7281, IMM, 13402 Marseille Cedex 20, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Rousset
authors.affiliation: CNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, UMR 7281, IMM, 13402 Marseille Cedex 20, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Brugna
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003576:Electron Transport Complex IV
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