title: Impact of light-exposure on the metabolite balance of transgenic potato tubers with modified glycoalkaloid biosynthesis.
abstract: Metabolite profiling (liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC-MS)) was used to assess the impact of light on the composition of transgenic potato (Solanum tuberosum L. cv. Desirée) with reduced glycoalkaloid content via the down-regulation of the SGT1 gene. Transgenic tubers exhibited an almost complete knock-out of α-solanine production and light had little impact on its accumulation. Levels of α-chaconine increased significantly in the peel of both the control and transgenic lines when exposed to light, particularly in the transgenic line. Major differences in metabolite profiles existed between outer and inner tuber tissues, and between light and dark-treated tubers. Many of the light-induced changes are explicable in terms of pathways known to be affected by stress responses. The impact of transgenesis on profiles was much less than that of tissue type or light and most differences were explicable in terms of the modification to the glycoalkaloid pathway.
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom. Electronic address: louise.shepherd@hutton.ac.uk.
authors.forename: Louise Vida Traill
authors.initials: LV
authors.lastname: Shepherd
authors.affiliation: Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Christine Anne
authors.initials: CA
authors.lastname: Hackett
authors.affiliation: Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Colin James
authors.initials: CJ
authors.lastname: Alexander
authors.affiliation: Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: James William
authors.initials: JW
authors.lastname: McNicol
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Julia Anne
authors.initials: JA
authors.lastname: Sungurtas
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Diane
authors.initials: D
authors.lastname: McRae
authors.affiliation: USDA, Agricultural Research Service, Crop Improvement and Genetics, Western Regional Research Centre, 800 Buchanan St, Albany, CA 94710-1105, USA.
authors.forename: Kent Frank
authors.initials: KF
authors.lastname: McCue
authors.affiliation: USDA, Agricultural Research Service, Crop Improvement and Genetics, Western Regional Research Centre, 800 Buchanan St, Albany, CA 94710-1105, USA.
authors.forename: William Richardson
authors.initials: WR
authors.lastname: Belknap
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Howard Vivian
authors.initials: HV
authors.lastname: Davies
mesh_terms: D002734:Chlorophyll / Q000032:analysis; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008027:Light; D013058:Mass Spectrometry; D035281:Plant Tubers / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D012991:Solanaceous Alkaloids / Q000096:biosynthesis*; D012992:Solanine / Q000031:analogs & derivatives / Q000032:analysis; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*
chemical_list: D012991:Solanaceous Alkaloids; C514919:alpha-solanine; D002734:Chlorophyll; D012992:Solanine; C011860:alpha-chaconine
keywords: Genetic modification; Glycoalkaloids; Light; Metabolomics; Potato (Solanum tuberosum); UDP-galactose:solanidine galactosyltransferase