title: Activation of the cAMP/protein kinase A signalling pathway by coronin 1 is regulated by cyclin-dependent kinase 5 activity.
abstract: Coronins constitute a family of conserved proteins expressed in all eukaryotes that have been implicated in the regulation of a wide variety of cellular activities. Recent work showed an essential role for coronin 1 in the modulation of the cAMP/PKA pathway in neurons through the interaction of coronin 1 with the G protein subtype Gαs in a stimulus-dependent manner, but the molecular mechanism regulating coronin 1-Gαs interaction remains unclear. We here show that phosphorylation of coronin 1 on Thr(418/424) by cyclin-dependent kinase (CDK) 5 activity was responsible for coronin 1-Gαs association and the modulation of cAMP production. Together these results show an essential role for CDK5 activity in promoting the coronin 1-dependent cAMP/PKA pathway.
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Somdeb
authors.initials: S
authors.lastname: BoseDasgupta
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Jayachandran
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Vera
authors.initials: V
authors.lastname: Studer
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Rühl
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Stiess
authors.affiliation: Biozentrum, University of Basel, Switzerland.
authors.forename: Jean
authors.initials: J
authors.lastname: Pieters
mesh_terms: D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000096:biosynthesis; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D004789:Enzyme Activation; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D015398:Signal Transduction
chemical_list: D008840:Microfilament Proteins; C072061:coronin proteins; D000242:Cyclic AMP; D051360:Cyclin-Dependent Kinase 5; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: CDK5; Coronin 1; G protein alpha S; Phosphorylation; cAMP/PKA signalling