title: Clinicopathological Significance of Osteopontin in Cholangiocarcinoma Cases.
abstract: Cholangiocarcinoma (CCA) is generally a rare primary liver tumor of the bile duct with extremely poor clinical outcomes due to late diagnosis. Osteopontin (OPN) is the most abundant expressed gene in intrahepatic CCA and its involvement in tumor aggressiveness suggests it could be a useful prognostic biomarker. However, the prognostic significance of OPN expression in CCA is still controversial. We therefore immunohistochemically studied OPN expression in 354 resected CCAs and correlated the results with patient clinicopathological parameters. OPN expression was separately scored according to the percentage of cancer cells or degree of stromal tissue staining and classified as low (score 0-1) and high (score 2-3). OPN expression in CCA cells was found in 177 out of 354 patients (56.5%), whereas stroma was positive in 185 out of 354 patients (52.3%). Univariate analysis with several of the aforementioned parameters revealed that stromal but not cancer cell OPN expression was significantly associated with tumor size, tumor direct invasion into normal liver parenchyma, regional lymph node metastasis and higher staging. The combination of cancer cell and stromal OPN expression demonstrated a positive trend for linkage with lymph node metastasis. Multivariate analysis identified gender, the presence of lymphatic permeation and lymph node metastasis, but not OPN expression, as independent prognostic factors. This study confirms the presence of stromal OPN expression in tumor aggressiveness but not survival in CCA patients.
authors.affiliation: Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand E-mail : banchob@kku.ac.th.
authors.forename: Marut
authors.initials: M
authors.lastname: Laohaviroj
authors.forename: Yaovalux
authors.initials: Y
authors.lastname: Chamgramol
authors.forename: Chawalit
authors.initials: C
authors.lastname: Pairojkul
authors.forename: Jason
authors.initials: J
authors.lastname: Mulvenna
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
mesh_terms: D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000378:metabolism*; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D053495:Osteopontin
references.citation: J Carcinog. 2015 Feb 23;14:1
references.pmid: 25788866
references.citation: Asian Pac J Cancer Prev. 2013;14(2):1107-10
references.pmid: 23621195
references.citation: J Clin Diagn Res. 2015 Jun;9(6):ZE10-3
references.pmid: 26266236
references.citation: J Hepatobiliary Pancreat Sci. 2014 Aug;21(8):521-4
references.pmid: 24464976
references.citation: Biomarkers. 2014 Sep;19(6):437-43
references.pmid: 24857368
references.citation: Clin Gastroenterol Hepatol. 2009 Nov;7(11 Suppl):S68-78
references.pmid: 19896103
references.citation: Tumori. 2009 Jan-Feb;95(1):48-52
references.pmid: 19366056
references.citation: J Hepatol. 2002 Dec;37(6):806-13
references.pmid: 12445422
references.citation: World J Gastroenterol. 2008 Apr 28;14(16):2501-10
references.pmid: 18442196
references.citation: Asian Pac J Cancer Prev. 2012;13(10):5161-5
references.pmid: 23244128
references.citation: J Cancer Res Clin Oncol. 2014 Dec;140(12):2143-56
references.pmid: 25009318
references.citation: Asian Pac J Cancer Prev. 2010;11(5):1159-66
references.pmid: 21198257
references.citation: Clin Exp Metastasis. 2004;21(6):515-23
references.pmid: 15679049
references.citation: World J Hepatol. 2010 Dec 27;2(12):419-27
references.pmid: 21191517
references.citation: J Hepatobiliary Pancreat Surg. 1999;6(2):128-35
references.pmid: 10398899
references.citation: Adv Parasitol. 2010;72:305-50
references.pmid: 20624536
references.citation: Diagn Pathol. 2011 Oct 13;6:98
references.pmid: 21992455
references.citation: Exp Oncol. 2004 Sep;26(3):179-84
references.pmid: 15494684
references.citation: Int J Parasitol. 2010 Aug 15;40(10):1203-12
references.pmid: 20493868
references.citation: Acta Trop. 2011 Sep;120 Suppl 1:S158-68
references.pmid: 20655862
references.citation: World J Gastroenterol. 2005 Jan 14;11(2):249-54
references.pmid: 15633225
references.citation: Hepatology. 2013 Dec;58(6):1992-2000
references.pmid: 23775819
references.citation: Liver Int. 2004 Feb;24(1):38-45
references.pmid: 15101999
references.citation: Oncology. 2009;76(4):293-300
references.pmid: 19262069
references.citation: Semin Liver Dis. 2004 May;24(2):115-25
references.pmid: 15192785
references.citation: Methods Mol Med. 2005;103:89-101
references.pmid: 15542899
references.citation: Cell Motil Cytoskeleton. 1999;43(4):269-87
references.pmid: 10423269
references.citation: Cancer. 2011 Aug 1;117(15):3393-403
references.pmid: 21287531