title: The smell of "anxiety": Behavioral modulation by experimental anosmia in zebrafish.
abstract: Olfaction is strongly involved in the regulation of fish behavior, including reproductive, defensive, social and migration behaviors. In fish, anosmia (the lack of olfaction) can be induced experimentally, impairing their ability to respond to various olfactory stimuli. Here, we examine the effects of experimental lidocaine-induced anosmia on anxiety-like behavior and whole-body cortisol levels in adult zebrafish (Danio rerio). We show that experimentally-induced anosmia reduces anxiolytic-like behavioral effects of fluoxetine and seems to interact with anxiogenic effect of stress also paralleling cortisol responses in zebrafish. These findings provide first experimental evidence that temporary anosmia modulates anxiety-like behaviors and physiology in adult zebrafish.
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS 97105-900, Brazil.
authors.forename: Murilo S
authors.initials: MS
authors.lastname: Abreu
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS 97105-900, Brazil; Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS 99052-900, Brazil.
authors.forename: Ana C V V
authors.initials: AC
authors.lastname: Giacomini
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University (GDOU), Zhanjiang, Guangdong 524025, China; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg 199034, Russia; Institutes of Chemical Technology and Natural Sciences, Ural Federal University, Ekaterinburg, 620002, Russia; ZENEREI Institute, 309 Palmer Court, Slidell, LA 70458, USA; The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell 70458, USA.
authors.forename: Allan V
authors.initials: AV
authors.lastname: Kalueff
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS 97105-900, Brazil; Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS 99052-900, Brazil; Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS 99052-900, Brazil; The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell 70458, USA. Electronic address: lbarcellos@upf.br.
authors.forename: Leonardo J G
authors.initials: LJ
authors.lastname: Barcellos
mesh_terms: D000704:Analysis of Variance; D000779:Anesthetics, Local / Q000633:toxicity; D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000627:therapeutic use; D001007:Anxiety / Q000188:drug therapy / Q000209:etiology*; D004195:Disease Models, Animal; D005260:Female; D005473:Fluoxetine / Q000627:therapeutic use; D006854:Hydrocortisone / Q000378:metabolism; D008012:Lidocaine / Q000633:toxicity; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D008297:Male; D009812:Odorants; D000857:Olfaction Disorders / Q000139:chemically induced / Q000150:complications*; D018709:Statistics, Nonparametric; D015027:Zebrafish
chemical_list: D000779:Anesthetics, Local; D018687:Antidepressive Agents, Second-Generation; D005473:Fluoxetine; D008012:Lidocaine; D006854:Hydrocortisone
keywords: Fluoxetine; Food odor; Novel tank; Olfactory sense; Stress