title: Effective multiple stages continuous acetone-butanol-ethanol fermentation by immobilized bioreactors: Making full use of fresh corn stalk.
abstract: In order to make full use of the fresh corn stalk, the sugar containing juice was used as the sole substrate for acetone-butanol-ethanol production without any nutrients supplement, and the bagasse after squeezing the juice was used as the immobilized carrier. A total 21.34g/L of ABE was produced in batch cells immobilization system with ABE yield of 0.35g/g. A continuous fermentation containing three stages with immobilized cells was conducted and the effect of dilution rate on fermentation was investigated. As a result, the productivity and ABE solvents concentration reached 0.80g/Lh and 19.93g/L, respectively, when the dilution rate in each stage was 0.12/h (corresponding to a dilution rate of 0.04/h in the whole system). And the long-term operation indicated the continuous multiple stages ABE fermentation process had good stability and showed the great potential in future industrial applications.
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China; Beijing Tiantian Biological Products Corporation Limited, Beijing 100176, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Cai
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Changjing
authors.initials: C
authors.lastname: Chen
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Fu
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: qinpeiyong@tsinghua.org.cn.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Qin
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D000096:Acetone / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D018914:Cells, Immobilized; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D012997:Solvents; D003313:Zea mays
chemical_list: D000440:Butanols; D012997:Solvents; D000096:Acetone; D000431:Ethanol
keywords: ABE fermentation; Cell immobilization; Corn stalk bagasse; Corn stalk juice