title: Assessment of imidacloprid-induced mutagenic effects in somatic cells of Swiss albino male mice.
abstract: Pesticides are being used for plant protection to increase food protection and to reduce insect-borne diseases worldwide. Exposure to the pesticides may cause genotoxic effects on both the target and nontarget organisms, including man. Therefore, the mutagenicity evaluation of such pesticides has become a priority area of research. Imidacloprid (IMI), a neonicotinoid insecticide, is widely used in agriculture either alone or in combination with other insecticides. A combined approach employing micronucleus test (MNT) and chromosomal aberrations assay (CA) was utilized to assess the mutagenicity of imidacloprid in bone marrow of Swiss albino male mice. IMI suspension was prepared in 3% gum acacia and administered at doses of 5.5, 11 and 22 mg/kg body weight for 7, 14 and 28 days to mice. IMI treatment resulted in a dose and time-dependant increase in the frequencies of micronuclei per cell and chromosomal aberrations in bone marrow cells. A statistically significant increase in chromosomal aberrations and micronuclei/cell was found only after daily treatment of IMI at highest selected dose (22 mg/kg body weight) for longest selected time period (28 days) compared to the control group. Thus, daily exposure of imidacloprid at a dose level of 22 mg/kg body weight for 28 days caused mutagenic effects on the somatic cells of Swiss albino male mice.
authors.affiliation: a Department of Veterinary Pharmacology and Toxicology and.
authors.forename: Preeti
authors.initials: P
authors.lastname: Bagri
authors.affiliation: a Department of Veterinary Pharmacology and Toxicology and.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: b Department of Animal Genetics and Breeding , College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences , Hisar , Haryana , India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sikka
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D007093:Imidazoles / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008677:Metaphase / Q000187:drug effects / Q000235:genetics; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*
chemical_list: D007093:Imidazoles; D007306:Insecticides; D009153:Mutagens; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid
keywords: Mutagenicity; chromosomal aberration; imidacloprid (CAS: 138261-41-3); mice; micronucleus