title: MicroRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1.
abstract: MicroRNAs (miRNAs) play essential roles in muscle cell proliferation and differentiation. The muscle-specific miRNAs miR-1 and miR-206 have been shown to regulate muscle development and promote myogenic differentiation; however, it is likely that a number of other miRNAs play important roles in regulating myogenesis as well. microRNA-128 (miR-128) has been reported to be highly expressed in brain and skeletal muscle, and we found that miR-128 is also up-regulated during bovine skeletal muscle satellite cell differentiation using microarray analysis and qRT-PCR. However, little is known about the functions of miR-128 in bovine skeletal muscle satellite cell development. In this study, we investigated the biological functions of miR-128 in bovine skeletal muscle cell development. Using a dual-luciferase reporter assay, we confirmed that miR-128 regulates the Sp1 gene. Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. Inhibition of miR-128 increased Sp1 protein levels and promoted muscle satellite cell differentiation but also suppressed proliferation. Changes in miR-128 and Sp1 expression levels also affected the protein levels of MyoD and CDKN1A. Sp1, an activator of MyoD and a suppressor of CDKN1A, plays an important role in bovine muscle cell proliferation and differentiation. The results of our study reveal a mechanism by which miR-128 regulates bovine skeletal muscle satellite cell proliferation and myogenic differentiation via Sp1.
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dai
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Wei Ran
authors.initials: WR
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Yi Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Xin Feng
authors.initials: XF
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. xiangbinding@163.com.
authors.forename: Xiang Bin
authors.initials: XB
authors.lastname: Ding
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. guohong64@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering; D016329:Sp1 Transcription Factor
keywords: Bovine; Microrna-128; Myogenic differentiation; Skeletal muscle satellite cells; Sp1
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