title: Preparation of pH-sensitive zwitterionic nano micelles and drug controlled release for enhancing cellular uptake.
abstract: Zwitterionic copolymers have exhibited high resistance to nonspecific protein adsorption and have wide applications in drug delivery systems. Herein, a pH-responsive poly(Lysine-alt-N,N'-bis(acryloyl) diaminohexane) was synthesized through the Michael addition polymerization between N, N'-bis(acryloyl) diaminohexane and lysine. Subsequently, nano micelles (NMs) were formed by self-assembly of the copolymer in an aqueous solution. The NMs showed a slightly negative charge in blood environment, but a positively charged surface in extracellular pH of tumor. This feature could be used to enhance permeability and retention effect, and reinforce tumor cell uptake. Vitro release studies revealed that the release of DOX from the DOX-loaded NMs was evidently faster at pH 5.0 than at pH 7.4. MTT assays revealed that NMs were nontoxic. Thus, these smart NMs were feasible candidates and could be potentially used in cancer chemotherapy.
authors.affiliation: a The Key Laboratory of Food Colloids and Biotechnology, School of Chemical and Material Engineering, Ministry of Education , Jiangnan University , Wuxi , China.
authors.forename: Luyan
authors.initials: L
authors.lastname: Wu
authors.affiliation: a The Key Laboratory of Food Colloids and Biotechnology, School of Chemical and Material Engineering, Ministry of Education , Jiangnan University , Wuxi , China.
authors.forename: Caihua
authors.initials: C
authors.lastname: Ni
authors.affiliation: a The Key Laboratory of Food Colloids and Biotechnology, School of Chemical and Material Engineering, Ministry of Education , Jiangnan University , Wuxi , China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a The Key Laboratory of Food Colloids and Biotechnology, School of Chemical and Material Engineering, Ministry of Education , Jiangnan University , Wuxi , China.
authors.forename: Gang
authors.initials: G
authors.lastname: Shi
mesh_terms: D016475:3T3 Cells; D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism; D001692:Biological Transport; D002417:Cattle; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; C000612849:poly(lysine-alt-N,N'-bis(acryloyl)diaminohexane); D014867:Water; D011107:Polylysine; D004317:Doxorubicin
keywords: Zwitterionic polymer; doxorubicin; drug release; pH-responsive