title: Pretreatment of Cardiac Stem Cells With Exosomes Derived From Mesenchymal Stem Cells Enhances Myocardial Repair.
abstract: BACKGROUND
  Exosomes derived from mesenchymal stem cells (MSCs) were proved to boost cell proliferation and angiogenic potency. We explored whether cardiac stem cells (CSCs) preconditioned with MSC exosomes could survive and function better in a myocardial infarction model.
  
  
  RESULTS
  DiI-labeled exosomes were internalized with CSCs. They stimulated proliferation, migration, and angiotube formation of CSCs in a dose-dependent manner. In a rat myocardial infarction model, MSC exosome-preconditioned CSCs had significantly better survival, enhanced capillary density, reduced cardiac fibrosis, and restored long-term cardiac function. MicroRNA profiling analysis revealed that a set of microRNAs were significantly changed in CSCs after MSC exosome treatment.
  
  
  CONCLUSIONS
  Pretreatment of CSCs with MSC exosomes provided a promising strategy to improve survival and angiogenic potency of CSCs.
authors.affiliation: Department of Cardiovascular Surgery of The First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, China (Z.Z., J.Y., W.Y., Y.L., Z.S.).
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Surgery of The First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, China (Z.Z., J.Y., W.Y., Y.L., Z.S.).
authors.forename: Junjie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Cardiovascular Surgery of The First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, China (Z.Z., J.Y., W.Y., Y.L., Z.S.).
authors.forename: Weiya
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Cardiovascular Surgery of The First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, China (Z.Z., J.Y., W.Y., Y.L., Z.S.).
authors.forename: Yangxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Surgery of The First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, China (Z.Z., J.Y., W.Y., Y.L., Z.S.).
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Regenerative Medicine, Tokai University School of Medicine, Kanagawa, Japan (T.A.).
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asahara
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D066026:Endothelial Progenitor Cells / Q000378:metabolism / Q000637:transplantation*; D055354:Exosomes / Q000378:metabolism*; D005355:Fibrosis; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D018919:Neovascularization, Physiologic; D010641:Phenotype; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012038:Regeneration; D015398:Signal Transduction; D033581:Stem Cell Transplantation / Q000379:methods*; D013318:Stroke Volume; D013997:Time Factors; D016277:Ventricular Function, Left
chemical_list: D035683:MicroRNAs
keywords: angiogenesis; cardiac stem cells; exosomes; miRNA profiling; proliferation
references.citation: Stem Cells. 2014 Mar;32(3):674-83
references.pmid: 24022988
references.citation: Circ Res. 2010 Oct 1;107(7):913-22
references.pmid: 20671238
references.citation: Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17260-5
references.pmid: 25404316
references.citation: Cardiovasc Res. 2005 Jul 1;67(1):30-8
references.pmid: 15878159
references.citation: Stem Cell Res. 2010 May;4(3):214-22
references.pmid: 20138817
references.citation: J Hepatol. 2009 Apr;50(4):766-78
references.pmid: 19232449
references.citation: Oncol Rep. 2012 Dec;28(6):1935-44
references.pmid: 22993103
references.citation: Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17783-8
references.pmid: 17965233
references.citation: Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):559-63
references.pmid: 22203988
references.citation: Circulation. 2013 Jan 15;127(2):213-23
references.pmid: 23224061
references.citation: Am J Physiol Heart Circ Physiol. 2015 Aug 1;309(3):H471-80
references.pmid: 26055795
references.citation: Can J Cardiol. 2014 Nov;30(11):1288-98
references.pmid: 25092406
references.citation: Arterioscler Thromb Vasc Biol. 2014 Jun;34(6):1114-9
references.pmid: 24828515
references.citation: Cardiovasc Res. 2011 Aug 1;91(3):402-11
references.pmid: 21345805
references.citation: Cancer Lett. 2009 Mar 18;275(2):256-65
references.pmid: 19036499
references.citation: Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8966-71
references.pmid: 15951423
references.citation: J Mol Med (Berl). 2014 Apr;92(4):387-97
references.pmid: 24337504
references.citation: Cardiovasc Res. 2014 Sep 1;103(4):530-41
references.pmid: 25016614
references.citation: Stem Cells Int. 2015;2015:482171
references.pmid: 26089917
references.citation: Cell. 2003 Sep 19;114(6):763-76
references.pmid: 14505575
references.citation: Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):13051-6
references.pmid: 26438843
references.citation: Nature. 2014 May 15;509(7500):337-41
references.pmid: 24805242
references.citation: Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14068-73
references.pmid: 17709737
references.citation: FASEB J. 2006 Apr;20(6):661-9
references.pmid: 16581974
references.citation: Exp Cell Res. 2011 May 1;317(8):1192-202
references.pmid: 21276792
references.citation: Mol Cell Biochem. 2013 Nov;383(1-2):13-20
references.pmid: 23812844
references.citation: Lab Invest. 2009 Nov;89(11):1317-28
references.pmid: 19786948
references.citation: J Am Coll Cardiol. 2015 Nov 3;66(18):1990-9
references.pmid: 26516002
references.citation: Circulation. 2013 Jul 9;128(2):122-31
references.pmid: 23757309
references.citation: J Mol Signal. 2011 Oct 05;6:12
references.pmid: 21970630
references.citation: Circulation. 2009 Sep 8;120(10):876-87
references.pmid: 19704095
references.citation: J Mol Cell Cardiol. 2007 Jun;42(6):1036-44
references.pmid: 17498737
references.citation: Angiogenesis. 2012 Mar;15(1):33-45
references.pmid: 22203239
references.citation: Blood. 2004 Dec 1;104(12):3581-7
references.pmid: 15297308
references.citation: FASEB J. 2010 May;24(5):1467-78
references.pmid: 20019242