title: Borrelia bissettiaesp. nov. andBorrelia californiensissp. nov. prevail in diverse enzootic transmission cycles.
abstract: Two species of the genusBorrelia,Borrelia bissettiaesp. nov. andBorrelia californiensissp. nov., were first described by Postic and co-workers on the basis of genetic analyses of several loci. Multilocus sequence analysis of eight housekeeping loci confirmed that these twoBorreliagenomospecies are distinct members of theBorrelia burgdorferi sensu latocomplex.B. bissettiaesp. nov. was initially described in transmission cycles involvingNeotoma fuscipeswood rats andIxodes pacificusticks in California, andNeotoma mexicanaandIxodes spinipalpisin Colorado. The preferred host ofB. californiensissp. nov. appears to be the California kangaroo rat,Dipodomys californicus;Ixodes jellisoni,I. spinipalipisandI. pacificusticks are naturally infected with it. Thus, the ecological associations of the two genomospecies and their genetic distance from all other knownBorreliagenomospecies species justify their description as separate genomospecies:B. bissettiaesp. nov. (type strain DN127T= DSM 17990T=  CIP 109136T) andB. californiensis(type strain CA446T= DSM 17989T= ATCC BAA-2689T).
authors.affiliation: German National Reference Centre for Borrelia at the Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Margos
authors.affiliation: University of California, Department of Environmental Science, Policy and Management, Berkeley, CA, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Lane
authors.affiliation: University of California, Department of Environmental Science, Policy and Management, Berkeley, CA, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Fedorova
authors.affiliation: German National Reference Centre for Borrelia at the Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Koloczek
authors.affiliation: Centers for Disease Control and Prevention, Vector-borne disease Branch, Fort Collins, CO, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Piesman
authors.affiliation: Centers for Disease Control and Prevention, Vector-borne disease Branch, Fort Collins, CO, USA.
authors.forename: Andrias
authors.initials: A
authors.lastname: Hojgaard
authors.affiliation: German National Reference Centre for Borrelia at the Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Sing
authors.affiliation: German National Reference Centre for Borrelia at the Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Fingerle
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