title: Assessing the toxicity of sediments using the medaka embryo-larval assay and 2 other bioassays.
abstract: Sediments are sinks for aquatic pollutants, and analyzing toxicity in such complex matrices is still challenging. To evaluate the toxicity of bioavailable pollutants accumulated in sediments from the Bizerte lagoon (Tunisia), a novel assay, the medaka embryo-larval assay by sediment contact, was applied. Japanese medaka (Oryzias latipes) embryos were incubated in direct contact with sediment samples up to hatching. Lethal and sublethal adverse effects were recorded in embryos and larvae up to 20 d postfertilization. Results from medaka embryo-larval assay were compared with cytotoxicity (Microtox®), genotoxicity (SOS chromotest), and pollutant content of sediments. The results highlight differences in the contamination profile and toxicity pattern between the different studied sediments. A significant correlation was shown between medaka embryo-larval assay by sediment contact and SOS chromotest responses and concentrations of most organic pollutants studied. No correlation was shown between pollutant levels and Microtox. According to the number of sediment samples detected as toxic, medaka embryo-larval assay by sediment contact was more sensitive than Microtox, which in turn was more sensitive than the SOS chromotest; and medaka embryo-larval assay by sediment contact allowed sediment toxicity assessment of moderately polluted sediments without pollutant extraction and using an ecologically realistic exposure scenario. Although medaka embryo-larval assay by sediment contact should be tested on a larger sample set, the results show that it is sensitive and convenient enough to monitor the toxicity of natural sediments. Environ Toxicol Chem 2016;35:2270-2280. © 2016 SETAC.
authors.affiliation: Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Tunisia.|Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Badreddine
authors.initials: B
authors.lastname: Barhoumi
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Clérandeau
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Landi
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Anaïk
authors.initials: A
authors.lastname: Pichon
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Florane
authors.initials: F
authors.lastname: Le Bihanic
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Poirier
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Anschutz
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.affiliation: Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Tunisia.
authors.forename: Mohamed Ridha
authors.initials: MR
authors.lastname: Driss
authors.affiliation: Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Cachot
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D004781:Environmental Exposure / Q000032:analysis*; D019015:Geologic Sediments / Q000737:chemistry*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D015999:Multivariate Analysis; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D009990:Oryzias / Q000196:embryology* / Q000235:genetics; D013014:SOS Response, Genetics / Q000187:drug effects; D012623:Seawater / Q000737:chemistry; D018675:Toxicity Tests; D014416:Tunisia; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical
keywords: Fish embryo-larval assay; Metal; Microtox; Organic pollutant; SOS chromotest; Sediment of the Bizerte lagoon