title: Morphological characterization and immunohistochemical detection of the proinflammatory cytokines IL-1β, IL-17A, and TNF-α in lung lesions associated with contagious bovine pleuropneumonia.
abstract: Contagious bovine pleuropneumonia (CBPP), a severe respiratory disease, is characterized by massive inflammation of the lung especially during the acute clinical stage of infection. Tissue samples from cattle, experimentally infected with Mycoplasma mycoides subsp. mycoides Afadé, were subjected to histopathological and immunohistochemical examination in order to provide insight into innate immune pathways that shape inflammatory host responses. Lung lesions were characterized by vasculitis, necrosis, and increased presence of macrophages and neutrophils, relative to uninfected animals. The presence of three cytokines associated with innate inflammatory immune responses, namely, IL-1β, IL-17A, and TNF-α, were qualitatively investigated in situ. Higher cytokine levels were detected in lung tissue samples from CBPP-affected cattle compared to samples derived from an uninfected control group. We therefore conclude that the cytokines TNF-α and IL-1β, which are prevalent in the acute phase of infections, play a role in the inflammatory response seen in the lung tissue in CBPP. IL-17A gets released by activated macrophages and attracts granulocytes that modulate the acute phase of the CBPP lesions.
authors.affiliation: Center for Experimental Medicine, University of Cologne, Medical School, University Hospital of Cologne, Robert-Koch-Straße 10, 50931, Cologne, Germany. anja.sterner-kock@uk-koeln.de.
authors.forename: Anja
authors.initials: A
authors.lastname: Sterner-Kock
authors.affiliation: Institut für Tierpathologie, Schönhauser Straße 62, 13127, Berlin, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Haider
authors.affiliation: Instituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Via Campo Boario, 64100, Teramo, Italy.
authors.forename: Flavio
authors.initials: F
authors.lastname: Sacchini
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100, Nairobi, Kenya.
authors.forename: Anne
authors.initials: A
authors.lastname: Liljander
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173, Hannover, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Meens
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100, Nairobi, Kenya.
authors.forename: Jane
authors.initials: J
authors.lastname: Poole
authors.affiliation: Center for Experimental Medicine, University of Cologne, Medical School, University Hospital of Cologne, Robert-Koch-Straße 10, 50931, Cologne, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Guschlbauer
authors.affiliation: Friedrich-Loeffler-Institut-Federal Research Institute for Animal Health, Naumburger Straße 96a, 07743, Jena, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Heller
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100, Nairobi, Kenya.
authors.forename: Jan
authors.initials: J
authors.lastname: Naessens
authors.affiliation: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100, Nairobi, Kenya.
authors.forename: Joerg
authors.initials: J
authors.lastname: Jores
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D007150:Immunohistochemistry / Q000662:veterinary; D053583:Interleukin-1beta / Q000032:analysis; D008168:Lung / Q000473:pathology; D009176:Mycoplasma mycoides / Q000276:immunology / Q000302:isolation & purification*; D011002:Pleuropneumonia, Contagious / Q000382:microbiology*; D053722:Receptors, Interleukin-17 / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D053583:Interleukin-1beta; D053722:Receptors, Interleukin-17; D014409:Tumor Necrosis Factor-alpha
keywords: CBPP; Contagious bovine pleuropneumonia; Cytokines; Histopathology; Immunocytochemistry; Mycoplasma mycoides subsp. mycoides
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