title: Mechanism Investigation of the Improvement of Chang Run Tong on the Colonic Remodeling in Streptozotocin-Induced Diabetic Rats.
abstract: Previous study demonstrated that Chang Run Tong (CRT) could partly restore the colon remodeling in streptozotocin- (STZ-) induced diabetic rats. Here we investigated the mechanisms of such effects of CRT. Diabetes was induced by a single injection of 40 mg/kg of STZ. CRT was poured into the stomach by gastric lavage once daily for 60 days. The remodeling parameters were obtained from diabetic (DM), CRT treated diabetic (T1, 50 g/kg; T2, 25 g/kg), and normal (Con) rats. Expressions of advanced glycation end product (AGE), AGE receptor, transforming growth factor-β1 (TGF-β1), and TGF-β1 receptor in the colon wall were immunochemically detected and quantitatively analyzed. The association between the expressions of those proteins and the remodeling parameters was analyzed. The expressions of those proteins were significantly higher in different colon layers in the DM group (P < 0.05, P < 0.01) and highly correlated to the remodeling parameters. Furthermore, the expressions of those proteins were significantly decreased in the T1 group (P < 0.05, P < 0.01) but not in the T2 group (P > 0.05). The corrective effect on the expressions of those proteins is likely to be one molecular pathway for the improvement of CRT on the diabetes-induced colon remodeling.
authors.affiliation: China-Japan Hospital, Beijing 100029, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sha
authors.affiliation: China-Japan Hospital, Beijing 100029, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Tong
authors.affiliation: Bioengineering College of Chongqing University, Chongqing 400044, China.
authors.forename: Hans
authors.identifier: 0000-0002-5792-2845
authors.initials: H
authors.lastname: Gregersen
authors.affiliation: Bioengineering College of Chongqing University, Chongqing 400044, China; Department of Clinical Medicine, Aarhus University, 8200 Aarhus N, Denmark.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003108:Colonic Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D048909:Diabetes Complications / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D013311:Streptozocin; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: C000593874:Ager protein, rat; D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D000067759:Receptor for Advanced Glycation End Products; D018125:Receptors, Transforming Growth Factor beta; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; C000611747:chang run tong; D013311:Streptozocin; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; C000625843:Tgfbr1 protein, rat
references.citation: Mol Nutr Food Res. 2015 May;59(5):927-38
references.pmid: 25655894
references.citation: J Diabetes. 2015 Jan;7(1):114-24
references.pmid: 24666836
references.citation: Ageing Res Rev. 2002 Feb;1(1):1-15
references.pmid: 12039445
references.citation: Perspect Vasc Surg Endovasc Ther. 2006 Mar;18(1):55-62
references.pmid: 16628336
references.citation: World J Gastroenterol. 2013 Dec 7;19(45):8247-51
references.pmid: 24363515
references.citation: J Altern Complement Med. 2009 Dec;15(12):1335-46
references.pmid: 19958146
references.citation: Methods Mol Biol. 2000;142:55-65
references.pmid: 10806613
references.citation: Diabetes. 2009 Jul;58(7):1659-67
references.pmid: 19401417
references.citation: World J Diabetes. 2015 May 15;6(4):662-72
references.pmid: 25987965
references.citation: Diabetes Care. 1996 Oct;19(10):1113-7
references.pmid: 8886558
references.citation: Mol Cell Biochem. 2010 Aug;341(1-2):33-41
references.pmid: 20309613
references.citation: FASEB J. 2004 Jan;18(1):176-8
references.pmid: 12709399
references.citation: FASEB J. 2004 Aug;18(11):1249-51
references.pmid: 15180953
references.citation: Osteoarthritis Cartilage. 2006 Dec;14(12):1227-36
references.pmid: 16824771
references.citation: BMC Complement Altern Med. 2013 Oct 03;13:251
references.pmid: 24090434
references.citation: J Mol Med (Berl). 2005 Nov;83(11):876-86
references.pmid: 16133426
references.citation: J Physiol. 2010 Oct 15;588(Pt 20):3971-82
references.pmid: 20807791
references.citation: Aliment Pharmacol Ther. 2010 May;31(9):938-49
references.pmid: 20180788
references.citation: World J Diabetes. 2013 Jun 15;4(3):51-63
references.pmid: 23772273
references.citation: Ann N Y Acad Sci. 2011 Dec;1243:88-102
references.pmid: 22211895
references.citation: Cell. 2003 Jun 13;113(6):685-700
references.pmid: 12809600
references.citation: Mol Vis. 2012;18:2749-57
references.pmid: 23213275
references.citation: Dig Dis Sci. 2009 Aug;54(8):1636-42
references.pmid: 18989775
references.citation: Gerontology. 2012;58(3):227-37
references.pmid: 22222677
references.citation: Best Pract Res Clin Gastroenterol. 2011 Feb;25(1):151-8
references.pmid: 21382586
references.citation: Exp Eye Res. 2007 Sep;85(3):406-12
references.pmid: 17678894
references.citation: World J Gastroenterol. 2012 Sep 21;18(35):4875-84
references.pmid: 23002359
references.citation: Exp Neurol. 2008 Jun;211(2):469-79
references.pmid: 18406405
references.citation: J Biomech. 2008;41(5):1044-52
references.pmid: 18222457
references.citation: Am Fam Physician. 2008 Jun 15;77(12):1697-702
references.pmid: 18619079
references.citation: Curr Pharm Des. 2008;14(10):946-52
references.pmid: 18473844
references.citation: Georgian Med News. 2006 Feb;(131):13-8
references.pmid: 16575121
references.citation: J Pharmacol Sci. 2012;118(1):99-108
references.pmid: 32092843
references.citation: Dig Dis Sci. 2012 Jan;57(1):48-57
references.pmid: 22057282
references.citation: Endocrinology. 2015 Jan;156(1):268-79
references.pmid: 25375034
references.citation: Trans Am Clin Climatol Assoc. 2001;112:27-32; discussion 33
references.pmid: 11413780
references.citation: Best Pract Res Clin Gastroenterol. 2011 Feb;25(1):103-18
references.pmid: 21382582
references.citation: Nat Clin Pract Gastroenterol Hepatol. 2008 Mar;5(3):162-71
references.pmid: 18268523
references.citation: Neurogastroenterol Motil. 1996 Dec;8(4):277-97
references.pmid: 8959733
references.citation: J Biomech. 2013 Apr 5;46(6):1128-34
references.pmid: 23403079
references.citation: Ann N Y Acad Sci. 2005 Jun;1043:598-604
references.pmid: 16037282
references.citation: Mol Cell Biochem. 2003 Oct;252(1-2):125-32
references.pmid: 14577585
references.citation: Curr Diabetes Rev. 2008 May;4(2):92-100
references.pmid: 18473756
references.citation: Neurogastroenterol Motil. 2009 Dec;21 Suppl 2:1-8
references.pmid: 19824933
references.citation: Best Pract Res Clin Gastroenterol. 2011 Feb;25(1):3-18
references.pmid: 21382575
references.citation: PLoS One. 2014 Apr 07;9(4):e94160
references.pmid: 24710116
references.citation: Am J Gastroenterol. 2011 Jan;106(1):120-9
references.pmid: 21045817
references.citation: Diabetologia. 1996 Aug;39(8):946-51
references.pmid: 8858217
references.citation: Metabolism. 2005 Mar;54(3):345-50
references.pmid: 15736112
references.citation: Dis Colon Rectum. 2012 Jan;55(1):18-25
references.pmid: 22156863
references.citation: Endokrynol Pol. 2013;64(5):384-96
references.pmid: 24186596
references.citation: J Physiol Pharmacol. 2005 Mar;56(1):3-13
references.pmid: 15795471
references.citation: World J Gastroenterol. 2008 May 7;14(17):2631-8
references.pmid: 18461648