title: Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 α1 expression.
abstract: Heat shock protein 60 (Hsp60) is a chaperone localizing in skeletal muscle mitochondria, whose role is poorly understood. In the present study, the levels of Hsp60 in fibres of the entire posterior group of hindlimb muscles (gastrocnemius, soleus, and plantaris) were evaluated in mice after completing a 6-week endurance training program. The correlation between Hsp60 levels and the expression of four isoforms of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α) were investigated only in soleus. Short-term overexpression of hsp60, achieved by in vitro plasmid transfection, was then performed to determine whether this chaperone could have a role in the activation of the expression levels of PGC1α isoforms. The levels of Hsp60 protein were fibre-type specific in the posterior muscles and endurance training increased its content in type I muscle fibers. Concomitantly with the increased levels of Hsp60 released in the blood stream of trained mice, mitochondrial copy number and the expression of three isoforms of PGC1α increased. Overexpressing hsp60 in cultured myoblasts induced only the expression of PGC1 1α, suggesting a correlation between Hsp60 overexpression and PGC1 1 α activation.
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Barone
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Macaluso
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Sangiorgi
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Campanella
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Marino Gammazza
authors.affiliation: Department of Anatomical, Histological, Forensic &Orthopaedic Sciences, Section of Histology &Medical Embryology, Sapienza University of Rome, Rome, 00161, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Moresi
authors.affiliation: Department of Anatomical, Histological, Forensic &Orthopaedic Sciences, Section of Histology &Medical Embryology, Sapienza University of Rome, Rome, 00161, Italy.|Interuniversity Institute of Myology, Rome, 00161, Italy.|University Pierre et Marie Curie Paris, UR4 Aging, Stress, Inflammation, 75005 Paris, France.
authors.forename: Dario
authors.initials: D
authors.lastname: Coletti
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, IMET, 21201 Baltimore, MD, USA.
authors.forename: Everly
authors.initials: E
authors.lastname: Conway de Macario
authors.affiliation: Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.|Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, IMET, 21201 Baltimore, MD, USA.
authors.forename: Alberto Jl
authors.initials: AJ
authors.lastname: Macario
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
authors.affiliation: Department of Anatomical, Histological, Forensic &Orthopaedic Sciences, Section of Histology &Medical Embryology, Sapienza University of Rome, Rome, 00161, Italy.|Interuniversity Institute of Myology, Rome, 00161, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Adamo
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.
authors.forename: Felicia
authors.initials: F
authors.lastname: Farina
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zummo
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, 90127 Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, 90100, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Di Felice
mesh_terms: D000818:Animals; D015415:Biomarkers; D002460:Cell Line; D018834:Chaperonin 60 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D055354:Exosomes / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010084:Oxidation-Reduction; D010805:Physical Conditioning, Animal; D010807:Physical Endurance; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D015415:Biomarkers; D018834:Chaperonin 60; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1
references.citation: Sports Med. 2009;39(8):643-62
references.pmid: 19769414
references.citation: Exp Gerontol. 2001 Feb;36(2):341-52
references.pmid: 11226747
references.citation: J Biol Chem. 2007 Jan 5;282(1):194-9
references.pmid: 17099248
references.citation: Aging Cell. 2008 Jan;7(1):101-11
references.pmid: 18031569
references.citation: Cell Stress Chaperones. 2014 May;19(3):343-53
references.pmid: 24057177
references.citation: Cell Stress Chaperones. 2011 Nov;16(6):689-93
references.pmid: 21748374
references.citation: Cell. 2012 Dec 7;151(6):1319-31
references.pmid: 23217713
references.citation: Biomed Res Int. 2014;2014:402175
references.pmid: 25136584
references.citation: PLoS One. 2010;5(2):e9247
references.pmid: 20169074
references.citation: J Appl Physiol Respir Environ Exerc Physiol. 1984 Apr;56(4):831-8
references.pmid: 6373687
references.citation: IUBMB Life. 2014 May;66(5):318-26
references.pmid: 24839203
references.citation: Annu Rev Cell Biol. 1993;9:601-34
references.pmid: 8280473
references.citation: Cancer Biol Ther. 2008 Jun;7(6):801-9
references.pmid: 18497565
references.citation: PLoS One. 2009;4(4):e5219
references.pmid: 19381331
references.citation: Acta Physiol (Oxf). 2013 Sep;209(1):26-33
references.pmid: 23710799
references.citation: PLoS One. 2009;4(5):e5570
references.pmid: 19440308
references.citation: Dis Model Mech. 2014 Apr;7(4):421-34
references.pmid: 24719117
references.citation: J Biol Chem. 1967 May 10;242(9):2278-82
references.pmid: 4290225
references.citation: Biol Rev Camb Philos Soc. 2013 Nov;88(4):955-87
references.pmid: 23551966
references.citation: Am J Physiol. 1996 Dec;271(6 Pt 1):C1828-37
references.pmid: 8997182
references.citation: Cell Stress Chaperones. 1999 Mar;4(1):29-35
references.pmid: 10467106
references.citation: Med Sci Sports Exerc. 2008 Jul;40(7):1255-62
references.pmid: 18580405
references.citation: Nat Protoc. 2008;3(6):1101-8
references.pmid: 18546601
references.citation: Cell Stress Chaperones. 2010 Mar;15(2):123-41
references.pmid: 19731087
references.citation: Biochim Biophys Acta. 2013 Feb;1833(2):388-99
references.pmid: 22705353
references.citation: J Strength Cond Res. 2007 Feb;21(1):193-8
references.pmid: 17313269
references.citation: Anat Rec A Discov Mol Cell Evol Biol. 2005 May;284(1):446-53
references.pmid: 15791579
references.citation: Am J Physiol Cell Physiol. 2003 Jun;284(6):C1669-77
references.pmid: 12734114
references.citation: Endocrinology. 2008 Sep;149(9):4527-33
references.pmid: 18511502
references.citation: Amino Acids. 2013 Dec;45(6):1331-41
references.pmid: 24178766
references.citation: J Tissue Eng Regen Med. 2015 Nov;9(11):E51-64
references.pmid: 23592297
references.citation: PLoS One. 2007;2(11):e1198
references.pmid: 18030332
references.citation: Exp Physiol. 2000 Jan;85(1):92-102
references.pmid: 10662898
references.citation: Cell Stress Chaperones. 2004 Winter;9(4):390-6
references.pmid: 15633297
references.citation: Muscle Nerve. 2004 May;29(5):656-62
references.pmid: 15116368
references.citation: Circulation. 2002 Jul 9;106(2):196-201
references.pmid: 12105158
references.citation: J Physiol. 2011 May 1;589(Pt 9):2129-38
references.pmid: 21224240
references.citation: Cancer Biol Ther. 2006 Jul;5(7):714-20
references.pmid: 16861898
references.citation: PLoS One. 2013;8(11):e79686
references.pmid: 24223995
references.citation: Cell Stress Chaperones. 2007 Autumn;12(3):265-74
references.pmid: 17915559
references.citation: Infect Dis Obstet Gynecol. 1999;7(3):128-32
references.pmid: 10371470
references.citation: Circulation. 2008 Dec 16;118(25):2687-93
references.pmid: 19106391
references.citation: J Hypertens. 2008 Dec;26(12):2389-92
references.pmid: 19008717
references.citation: FEBS Lett. 2013 May 2;587(9):1379-84
references.pmid: 23523921
references.citation: Int J Immunogenet. 2009 Feb;36(1):73-8
references.pmid: 19207939
references.citation: Cell Res. 2014 Jan;24(1):92-104
references.pmid: 24281265
references.citation: Med Sci Sports Exerc. 2000 Feb;32(2):365-9
references.pmid: 10694118
references.citation: Trends Mol Med. 2012 Oct;18(10):599-606
references.pmid: 22877884
references.citation: Exerc Sport Sci Rev. 2004 Jan;32(1):4-8
references.pmid: 14748542
references.citation: PLoS One. 2012;7(7):e42008
references.pmid: 22848686
references.citation: Circulation. 2000 Jul 4;102(1):14-20
references.pmid: 10880409
references.citation: Eur J Histochem. 2008 Oct-Dec;52(4):221-8
references.pmid: 19109096
references.citation: Nutr Diabetes. 2013 Sep 16;3:e88
references.pmid: 24042701
references.citation: Scand J Med Sci Sports. 2009 Dec;19(6):782-9
references.pmid: 18980603
references.citation: J Physiol. 2013 Mar 15;591(6):1551-61
references.pmid: 23297307