title: Differential DNA methylation patterns of homeobox genes in proximal and distal colon epithelial cells.
abstract: Region and cell-type specific differences in the molecular make up of colon epithelial cells have been reported. Those differences may underlie the region-specific characteristics of common colon epithelial diseases such as colorectal cancer and inflammatory bowel disease. DNA methylation is a cell-type specific epigenetic mark, essential for transcriptional regulation, silencing of repetitive DNA and genomic imprinting. Little is known about any region-specific variations in methylation patterns in human colon epithelial cells. Using purified epithelial cells and whole biopsies (n= 19) from human subjects, we generated epigenome-wide DNA methylation data (using the HELP-tagging assay), comparing the methylation signatures of the proximal and distal colon. We identified a total of 125 differentially methylated sites (DMS) mapping to transcription start sites of protein-coding genes, most notably several members of the homeobox (HOX) family of genes. Patterns of differential methylation were validated with MassArray EpiTYPER. We also examined DNA methylation in whole biopsies, applying a computational technique to deconvolve variation in methylation within cell types and variation in cell-type composition across biopsies. Including inferred epithelial proportions as a covariate in differential methylation analysis applied to the whole biopsies resulted in greater overlap with the results obtained from purified epithelial cells compared with when the covariate was not included. Results obtained from both approaches highlight region-specific methylation patterns of HOX genes in colonic epithelium. Regional variation in methylation patterns has implications for the study of diseases that exhibit regional expression patterns in the human colon, such as inflammatory bowel disease and colorectal cancer.
authors.affiliation: Clinical Pharmacology, School of Medicine, National University of Ireland, Galway, Ireland; School of Mathematics, Statistics and Applied Mathematics, National University of Ireland, Galway, Ireland; and.
authors.forename: Alan
authors.initials: A
authors.lastname: Barnicle
authors.affiliation: School of Mathematics, Statistics and Applied Mathematics, National University of Ireland, Galway, Ireland; and.
authors.forename: Cathal
authors.initials: C
authors.lastname: Seoighe
authors.affiliation: Center of Epigenomics and Department of Genetics (Division of Computational Genetics), Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Aaron
authors.initials: A
authors.lastname: Golden
authors.affiliation: Center of Epigenomics and Department of Genetics (Division of Computational Genetics), Albert Einstein College of Medicine, Bronx, New York.
authors.forename: John M
authors.initials: JM
authors.lastname: Greally
authors.affiliation: Clinical Pharmacology, School of Medicine, National University of Ireland, Galway, Ireland; laurence.egan@nuigalway.ie.
authors.forename: Laurence J
authors.initials: LJ
authors.lastname: Egan
mesh_terms: D001706:Biopsy; D003106:Colon / Q000166:cytology* / Q000502:physiology; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D004847:Epithelial Cells / Q000502:physiology; D005801:Genes, Homeobox; D006801:Humans; D015203:Reproducibility of Results
keywords: DNA methylation; deconvolution; epigenome-wide sequencing; region-specific colonic variation
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