title: Osteogenic effect of tricalcium phosphate substituted by magnesium associated with Genderm® membrane in rat calvarial defect model.
abstract: Beta-tricalcium phosphate (β-TCP) is one of the most widely employed bioresorbable materials for bone repair since it shows excellent biological compatibility, osteoconductivity and resorbability. The incorporation of divalent cations such as magnesium onto the β-TCP structure (β-TCMP) may improve the biological response to the material through the release of bioactive ions. The objective of this study was to evaluate, on a rat calvarial critical size grafting model, the bone regeneration process using β-TCP and β-TMCP granules by histomorphometric analysis. Results demonstrated that six months after bone grafting, the association of GBR (guided bone regeneration) using a membrane (GenDerm®) and granules of β-TCP and β-TCMP significantly improves bone repair in the treatment of critical-size defect in rat skulls, in comparison to untreated defects or GBR alone, leading to a bone level approximately four to five-fold greater than in the blood clot group. The β-TCMP+GenDerm® membrane group presented 40.5% of the defect area filled by newly-formed bone, even at the central part of the defect, rather than only at the border, as seen in the other experimental groups.
authors.affiliation: Department of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro/COPPE, Rio de Janeiro, RJ 21945-970, Brazil. Electronic address: neusacosta@infolink.com.br.
authors.forename: Neusa M F
authors.initials: NM
authors.lastname: Costa
authors.affiliation: Department of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro/COPPE, Rio de Janeiro, RJ 21945-970, Brazil.
authors.forename: Debora H
authors.initials: DH
authors.lastname: Yassuda
authors.affiliation: Department of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro/COPPE, Rio de Janeiro, RJ 21945-970, Brazil.
authors.forename: Marcia S
authors.initials: MS
authors.lastname: Sader
authors.affiliation: Post-graduation Program in Dentistry, Faculty of Odontology, Fluminense Federal University, Niteroi, RJ 24020-150, Brazil.
authors.forename: Gustavo V O
authors.initials: GV
authors.lastname: Fernandes
authors.affiliation: Department of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro/COPPE, Rio de Janeiro, RJ 21945-970, Brazil.
authors.forename: Glória D A
authors.initials: GD
authors.lastname: Soares
authors.affiliation: Cell Therapy Center, Clinical Research Unit, Fluminense Federal University, Niteroi, RJ 24030-210, Brazil; Bioengineering Program, National Institute of Metrology, Quality and Technology (INMETRO), Duque de Caxias, RJ 25250-020, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Granjeiro
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D005260:Female; D008274:Magnesium / Q000494:pharmacology*; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000378:metabolism / Q000473:pathology
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D008567:Membranes, Artificial; C485817:beta-tricalcium phosphate; D008274:Magnesium
keywords: Bone substitutes; Guided bone regeneration; Magnesium-substituted tricalcium phosphate; β-Tricalcium phosphate