title: Morphological and morphometric study of protective effect of green tea in gentamicin-induced nephrotoxicity in rats.
abstract: OBJECTIVE
  One of the most popular beverages worldwide, green tea, was investigated for its potential protective effect in a rat model of gentamicin-induced nephrotoxicity by monitoring functional and morphological changes in kidneys.
  
  
  METHODS
  The study was conducted on four groups of rats: control group (C), treated with only gentamicin (GM), treated with only green tea (GT) and treated with both gentamicin and green tea (GT+GM). Kidney function, oxidant and antioxidant parameters of renal tissue, as well as histopathological studies were assessed. Morphometric analysis was used to quantify these histopathological changes.
  
  
  RESULTS
  Gentamicin caused significant elevations in serum creatinine and urea and oxidative stress parameter (AOPP), while antioxidative enzyme catalase was significantly decreased. Histological sections of kidneys in GM group revealed necrosis of proximal tubules, vacuolation of cytoplasm and massive mononuclear inflammatory infiltrates in interstitium. Coadministration of green tea with gentamicin histologically showed renoprotective effect. Histological results were confirmed and quantified by morphometric analysis. Also in this group we measured ameliorated parameters of renal functions and antioxidative defense.
  
  
  CONCLUSIONS
  Regenerative potential of green tea after renal injury induced by gentamicin could be explained through the decrease of oxidative stress and lipid peroxidation. Green tea is a natural antioxidant, with many health promoting effects, widely available and in accordance to that affordable. Because of the established habits, people largely consume it as a beverage. It could be beneficial in the reduction of oxidative stress and changes caused by it primarily in renal tubules and interstitium.
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia. Electronic address: milica.veljkovic@gmail.com.
authors.forename: Milica
authors.initials: M
authors.lastname: Veljković
authors.affiliation: Department of Pharmacy, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Dragana R
authors.initials: DR
authors.lastname: Pavlović
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Nenad
authors.initials: N
authors.lastname: Stojiljković
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Ilić
authors.affiliation: Department of Histology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petrović
authors.affiliation: Department of Anatomy, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Radenković
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control*; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000187:drug effects / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005839:Gentamicins; D010936:Plant Extracts; D013662:Tea; D002374:Catalase
keywords: Gentamicin; Green tea; Nephrotoxicity; Oxidative stress