title: Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.
abstract: During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
authors.forename: Wan Beom
authors.identifier: 0000-0003-0022-9625
authors.initials: WB
authors.lastname: Park
authors.affiliation: Macrogen, Seoul, Korea.
authors.forename: Nak-Jung
authors.identifier: 0000-0003-0874-4146
authors.initials: NJ
authors.lastname: Kwon
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Pyoeng Gyun
authors.identifier: 0000-0001-6794-7918
authors.initials: PG
authors.lastname: Choe
authors.affiliation: Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
authors.forename: Su-Jin
authors.identifier: 0000-0001-8732-3950
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hong Sang
authors.identifier: 0000-0002-4535-6305
authors.initials: HS
authors.lastname: Oh
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Sang Min
authors.identifier: 0000-0002-1388-9318
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Macrogen, Seoul, Korea.
authors.forename: Hyonyong
authors.identifier: 0000-0001-6928-8805
authors.initials: H
authors.lastname: Chong
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jong-Il
authors.identifier: 0000-0002-7240-3744
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Kyoung-Ho
authors.identifier: 0000-0002-4517-3840
authors.initials: KH
authors.lastname: Song
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Ji Hwan
authors.identifier: 0000-0002-7628-1182
authors.initials: JH
authors.lastname: Bang
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Eu Suk
authors.identifier: 0000-0001-7132-0157
authors.initials: ES
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hong-Bin
authors.identifier: 0000-0001-6262-372X
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Sang Won
authors.identifier: 0000-0002-0550-1897
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
authors.forename: Nam Joong
authors.identifier: 0000-0001-6793-9467
authors.initials: NJ
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
authors.forename: Myoung-Don
authors.identifier: 0000-0002-2344-7695
authors.initials: MD
authors.lastname: Oh
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D004196:Disease Outbreaks; D006801:Humans; D008854:Microscopy, Electron; D065207:Middle East Respiratory Syndrome Coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D056910:Republic of Korea / Q000453:epidemiology; D017423:Sequence Analysis, RNA; D014709:Vero Cells
chemical_list: D012367:RNA, Viral
keywords: Korea; Microscopy, Electron; Middle East Respiratory Syndrome; Middle East Respiratory Syndrome Coronavirus; Phylogeny
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