title: Treatment of dilated cardiomyopathy in rabbits with mesenchymal stem cell transplantation and platelet-rich plasma.
abstract: Dilated cardiomyopathy (DCM) is a major cause of cardiovascular mortality and morbidity, and there is evidence to suggest that stem cell transplantation may be a viable treatment option for this condition. Therefore, the goal of the present study was to assess myocardial regeneration in rabbits with doxorubicin-induced DCM treated with adipose mesenchymal stem cells (MSC) alone or in combination with platelet-rich plasma (PRP). Twenty New Zealand rabbits received doxorubicin for the induction of DCM and were divided into four groups according to treatment: saline, MSC, PRP and MSC + RP. Treatment agents were injected directly into the left ventricular myocardium following a thoracoscopy. Rabbits were assessed through echocardiographic and electrocardiographic examinations, as well as serum cardiac troponin I measurements at baseline, after the induction of DCM and 15 days after treatment. Animals were euthanased following the last assessment, and hearts were collected for histopathological analyses. The MSC group showed improvements in all parameters assessed, while the PRP group showed significantly impaired heart function. Histopathology of the heart revealed that the MSC group displayed the lowest number of lesions, while rabbits in the MSC + PRP, saline and PRP groups had steadily advancing lesions. These results suggest that MSC transplantation can improve heart function in rabbits with DCM, and underscore the need for further studies of the effects of PRP on the myocardium.
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil; Laboratory of Embryology, Porto Alegre Clinical Hospital, Porto Alegre, Brazil. Electronic address: pdmvet@yahoo.com.br.
authors.forename: P D
authors.initials: PD
authors.lastname: Mörschbächer
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil.
authors.forename: T N
authors.initials: TN
authors.lastname: Alves Garcez
authors.affiliation: Laboratory of Embryology, Porto Alegre Clinical Hospital, Porto Alegre, Brazil.
authors.forename: A H
authors.initials: AH
authors.lastname: Paz
authors.affiliation: Laboratory of Embryology, Porto Alegre Clinical Hospital, Porto Alegre, Brazil.
authors.forename: A B
authors.initials: AB
authors.lastname: Magrisso
authors.affiliation: Laboratory of Embryology, Porto Alegre Clinical Hospital, Porto Alegre, Brazil.
authors.forename: H F
authors.initials: HF
authors.lastname: Mello
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil.
authors.forename: V M
authors.initials: VM
authors.lastname: Rolim
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil.
authors.forename: E B
authors.initials: EB
authors.lastname: Neuwald
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Driemeier
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil.
authors.forename: E A
authors.initials: EA
authors.lastname: Contesini
authors.affiliation: Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil; Laboratory of Embryology, Porto Alegre Clinical Hospital, Porto Alegre, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Cirne-Lima
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000139:chemically induced / Q000628:therapy*; D004317:Doxorubicin / Q000633:toxicity; D005260:Female; D006321:Heart / Q000502:physiology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011817:Rabbits; D012038:Regeneration
chemical_list: D004317:Doxorubicin
keywords: Cardiology; Cell therapy; Scaffold; Stem cell; Troponin I