title: Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection.
abstract: We identified a new class of deoxyribozymes named A-2 and A-3 by in vitro selection which required both Cu(2+) and Mn(2+) as cofactors that selectively and rapidly cleave the DNA substrate. Studies confirmed that they cleaved via a mechanistic pathway involving the formation of hydrogen peroxide as the reactive species. The kinetics, secondary structures and sequence tolerance of the new class of the deoxyribozymes A-2 and A-3 were reported.
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P. R. China. wmq3415@163.com.
authors.forename: Ming-Qi
authors.initials: MQ
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Dong
authors.forename: Huafan
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Tang
mesh_terms: D001483:Base Sequence; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D021881:DNA, Catalytic / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017433:Protein Structure, Secondary; D013379:Substrate Specificity
chemical_list: D021881:DNA, Catalytic; D008345:Manganese; D003300:Copper; D004247:DNA