title: Investigation of Therapeutic Effects of α-Mangostin on Thioacetamide-Induced Cirrhosis in Rats.
abstract: OBJECTIVE
  To determine the effects of alpha-mangostin on thioacetamide (TAA)-induced liver cirrhosis in rats.
  
  
  METHODS
  Male Wistar rats were divided into 3 groups and treated with intraperitoneal injections of TAA (200 mg/kg) 3 times per week for per week for 8, 12 and 16 weeks, respectively. One subgroup was left untreated whereas the other two were treated either with 100 mg/kg α-mangostin or vehicle alone (80% DMSO, 20% water), which were administered intraperitoneally 3 times per weekfor a total of4 weeks. The incidence offibrotic nodules on the liver and the serum levels of the liver enzymes aspartate transaminase (AST) and alanine transaminase (ALT) were measured. Moreover the liver cirrhosis-related genes expression and p53 protein level in liver were analyzed by quantitative reverse transcription PCR and Western blot analysis, respectively.
  
  
  RESULTS
  Fibrotic nodules on the liver were formed upon treatment with TAA for 12 or 16 weeks. The nodules were then reduced by treatment with α-mangostin as compared to treatment with the vehicle DMSO. Moreover, the serum levels of the liver enzymes AST and ALT after treatment with α-mangostin decreased as compared to DMSO alone. The liver cirrhosis-related genes expression showed no significant differences, whereas the p53 protein level in liver showed that α-mangostin reduced risk of liver fibrosis through the decrease in p53 expression as compared to the TAA_DMSO treatment.
  
  
  CONCLUSIONS
  The results suggest that α-mangostin has a beneficial therapeutic effect in the TAA liver cirrhosis model. Further investigations on mechanisms of α-mangostin as therapeutic agent should be determined.
authors.forename: Sukseree
authors.initials: S
authors.lastname: Supawadee
authors.forename: Sophonnithiprasert
authors.initials: S
authors.lastname: Thanet
authors.forename: Pradidarcheep
authors.initials: P
authors.lastname: Wisut
authors.forename: Nilbunga
authors.initials: N
authors.lastname: Somneuk
authors.forename: Nilwarangoon
authors.initials: N
authors.lastname: Sirinun
authors.forename: Watanapokasin
authors.initials: W
authors.lastname: Ramida
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D008103:Liver Cirrhosis / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013853:Thioacetamide / Q000633:toxicity*; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D044004:Xanthones; D013853:Thioacetamide; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C021053:mangostin