title: Label-free multi-photon imaging of dysplasia in Barrett's esophagus.
abstract: Barrett's esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of back-scattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett's.
authors.affiliation: College of Optical Sciences, University of Arizona,1630 E. University Blvd., Tucson, AZ 85721, USA.
authors.forename: Soroush
authors.initials: S
authors.lastname: Mehravar
authors.affiliation: College of Optical Sciences, University of Arizona,1630 E. University Blvd., Tucson, AZ 85721, USA; College of Medicine, University of Arizona, 1501 N. Campbell Avenue, Tucson, AZ 85724, USA; Department of Biomedical Engineering, University of Arizona, 1127 E. James E. Rogers Way, Tucson, AZ 85721, USA.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Banerjee
authors.affiliation: College of Medicine, University of Arizona, 1501 N. Campbell Avenue, Tucson, AZ 85724, USA.
authors.forename: Hemant
authors.initials: H
authors.lastname: Chatrath
authors.affiliation: College of Optical Sciences, University of Arizona,1630 E. University Blvd., Tucson, AZ 85721, USA.
authors.forename: Babak
authors.initials: B
authors.lastname: Amirsolaimani
authors.affiliation: College of Medicine, University of Arizona, 1501 N. Campbell Avenue, Tucson, AZ 85724, USA.
authors.forename: Krunal
authors.initials: K
authors.lastname: Patel
authors.affiliation: College of Medicine, University of Arizona, 1501 N. Campbell Avenue, Tucson, AZ 85724, USA.
authors.forename: Charmi
authors.initials: C
authors.lastname: Patel
authors.affiliation: College of Optical Sciences, University of Arizona,1630 E. University Blvd., Tucson, AZ 85721, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Norwood
authors.affiliation: College of Optical Sciences, University of Arizona,1630 E. University Blvd., Tucson, AZ 85721, USA.
authors.forename: Nasser
authors.initials: N
authors.lastname: Peyghambarian
authors.affiliation: College of Optical Sciences, University of Arizona,1630 E. University Blvd., Tucson, AZ 85721, USA.
authors.forename: Khanh
authors.initials: K
authors.lastname: Kieu
keywords: (020.4180) Multiphoton processes; (180.4315) Nonlinear microscopy; (180.5810) Scanning microscopy; (320.7090) Ultrafast lasers
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