title: Obesity-driven alterations in adipose-derived stem cells are partially restored by weight loss.
abstract: OBJECTIVE
  The therapeutic potential of adipose-derived stem cells (ASCs) is reduced by various stress-inducing conditions that affect tissue homeostasis such as diabetes, aging, and obesity. Previous works have provided evidence of negative effects of obesity on ASC populations, but it is unclear whether this persists after a weight loss. This study evaluated whether weight loss can restore the attenuated properties found in ASCs derived from populations with obesity (oASCs).
  
  
  METHODS
  In vitro functional analyses were performed to investigate the possible recovery properties in mouse oASCs. Using ASCs isolated from subcutaneous tissue from formerly obese mice (dASCs) and control mice (cASCs), cell proliferation, viability, and some regenerative properties in these cells were analyzed compared with oASCs to evaluate the functional cell state.
  
  
  RESULTS
  Cell proliferation, viability, and some regenerative properties are strengthened in dASCs and cASCs compared with oASCs. Nevertheless, metabolic analysis reveals a mitochondrial load misbalance and function leading to impaired respiration in dASCs.
  
  
  CONCLUSIONS
  This study demonstrates that an initial obese environment triggers a detrimental state in ASCs that is not completely recovered after weight loss.
authors.affiliation: Cardiac Development and Repair Department, Centro Nacional De Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Pérez
authors.affiliation: Cardiac Development and Repair Department, Centro Nacional De Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Suárez
authors.affiliation: Cardiac Development and Repair Department, Centro Nacional De Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Bernal
authors.affiliation: Cell Biology Group, Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), Barcelona, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: de Lucas
authors.affiliation: Cardiac Development and Repair Department, Centro Nacional De Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: San Martin
authors.affiliation: Cardiac Development and Repair Department, Centro Nacional De Investigaciones Cardiovasculares (CNIC), Madrid, Spain.|School of Doctorate and Research, European University, Madrid, Spain.
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Gálvez
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D051379:Mice; D009765:Obesity / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism*; D015431:Weight Loss