title: Hybrid phase ligation for efficient synthesis of histone proteins.
abstract: We introduce a hybrid solid-solution phase ligation approach that combines the efficiency of solid phase ligation with solution phase ligation in the total synthesis of modified histone proteins. A two linker strategy allows analysis throughout work on the solid phase and maximizes yields through cleavage at an external Rink, while an internal HMBA linker allows the native carboxyl terminus for any protein sequence. We demonstrate this approach for two histone proteins: triple-acetylated H4-K5ac, K12ac, K91ac and CENP-A-K124ac.
authors.affiliation: Department of Chemistry & Biochemistry, The Ohio State University, USA and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA. ottesen.1@osu.edu.
authors.forename: Ruixuan R
authors.initials: RR
authors.lastname: Yu
authors.affiliation: Department of Chemistry & Biochemistry, The Ohio State University, USA.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Mahto
authors.affiliation: Department of Chemistry & Biochemistry, The Ohio State University, USA and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA. ottesen.1@osu.edu.
authors.forename: Kurt
authors.initials: K
authors.lastname: Justus
authors.affiliation: Department of Chemistry & Biochemistry, The Ohio State University, USA.
authors.forename: Mallory M
authors.initials: MM
authors.lastname: Alexander
authors.affiliation: Department of Chemistry & Biochemistry, The Ohio State University, USA and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA. ottesen.1@osu.edu.
authors.forename: Cecil J
authors.initials: CJ
authors.lastname: Howard
authors.affiliation: Department of Chemistry & Biochemistry, The Ohio State University, USA and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA. ottesen.1@osu.edu.
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Ottesen
mesh_terms: D006657:Histones / Q000138:chemical synthesis* / Q000737:chemistry*; D008968:Molecular Conformation
chemical_list: D006657:Histones
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