title: Specific cellular accumulation of photofrin-II in EC cells promotes photodynamic treatment efficacy in esophageal cancer.
abstract: Photodynamic therapy (PDT), which uses a light-sensitive compound and laser irradiation, is a light-based oncological treatment modality. PDT offers an alternative, less invasive treatment for various malignant tumors, such as esophageal cancer (EC), through a photochemical reaction induced by photofrin-II or other oncotropic photosensitizers without severe complications. Previous studies has shown that cancerous tissues accumulated more photosensitizers than paired normal tissues, however, whether it is cellular or vascular mechanisms remains unknown. Herein, in vivo and in vitro examinations were performed to study the mechanisms by which photofrin-II effectively and specifically killed EC cells. In this study, EC tissue of patients treated with photofrin-II, human ESCC cellline SHEEC and parental normal cellline SHEE, primary culture cells of EC tissue were used. The concentration of photofrin-II in cells were evaluated by high-performance liquid chromatography (HPLC). The results exhibited that accumulation of photofrin-II in cancerous cells were significantly higher than that in non-cancerous cells (p<0.05) under certain dose and time period of incubation of photofrin-II. In summary, our study showed that, photofrin-II specifically accumulated in EC cells in vivo and in vitro after controlling for vascular factors, which provided strong evidence that maybe the cellular factor is the main mechanism by which photofrin-II-mediated PDT selectively caused EC cells death.
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Shegan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Liang
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Kaili
authors.initials: K
authors.lastname: Ding
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China. Electronic address: yingw_215@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China. Electronic address: 949898789@qq.com.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Feng
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D002460:Cell Line; D045744:Cell Line, Tumor; D002477:Cells / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017323:Dihematoporphyrin Ether / Q000032:analysis* / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000628:therapy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010778:Photochemotherapy; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: D017323:Dihematoporphyrin Ether
keywords: EC; High-performance liquid chromatography; Photodynamic therapy; Photofrin-II