title: Transfer of the methicillin resistance genomic island among staphylococci by conjugation.
abstract: Methicillin resistance creates a major obstacle for treatment of Staphylococcus aureus infections. The resistance gene, mecA, is carried on a large (20 kb to > 60 kb) genomic island, staphylococcal cassette chromosome mec (SCCmec), that excises from and inserts site-specifically into the staphylococcal chromosome. However, although SCCmec has been designated a mobile genetic element, a mechanism for its transfer has not been defined. Here we demonstrate the capture and conjugative transfer of excised SCCmec. SCCmec was captured on pGO400, a mupirocin-resistant derivative of the pGO1/pSK41 staphylococcal conjugative plasmid lineage, and pGO400::SCCmec (pRM27) was transferred by filter-mating into both homologous and heterologous S. aureus recipients representing a range of clonal complexes as well as S. epidermidis. The DNA sequence of pRM27 showed that SCCmec had been transferred in its entirety and that its capture had occurred by recombination between IS257/431 elements present on all SCCmec types and pGO1/pSK41 conjugative plasmids. The captured SCCmec excised from the plasmid and inserted site-specifically into the chromosomal att site of both an isogenic S. aureus and a S. epidermidis recipient. These studies describe a means by which methicillin resistance can be environmentally disseminated and a novel mechanism, IS-mediated recombination, for the capture and conjugative transfer of genomic islands.
authors.affiliation: Department of Internal Medicine, Virginia Commonwealth University School of Medicine, 1101 East Marshall St., Richmond, VA, 23298, USA.|Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, 1101 East Marshall St., Richmond, VA, 23298, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Ray
authors.affiliation: Department of Internal Medicine, Virginia Commonwealth University School of Medicine, 1101 East Marshall St., Richmond, VA, 23298, USA.
authors.forename: S
authors.initials: S
authors.lastname: Boundy
authors.affiliation: Department of Internal Medicine, Virginia Commonwealth University School of Medicine, 1101 East Marshall St., Richmond, VA, 23298, USA.|Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, 1101 East Marshall St., Richmond, VA, 23298, USA.
authors.forename: G L
authors.initials: GL
authors.lastname: Archer
mesh_terms: D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D044404:Genomic Islands; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D010957:Plasmids; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics
chemical_list: D004269:DNA, Bacterial
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