title: Inhibiting Matrix Metalloproteinase 3 Ameliorates Neuronal Loss in the Ganglion Cell Layer of Rats in Retinal Ischemia/Reperfusion.
abstract: It has been demonstrated that matrix metalloproteinase 3 (MMP3) is integrally involved in the neuronal degeneration of the central nervous system by promoting glial activation, neuronal apoptosis and damage to the brain-blood barrier. However, whether MMP3 also contributes to the neuronal degeneration induced by retinal ischemia/reperfusion is still uncertain. In the present study, we detected the cellular localization of MMP3 in adult rat retinae and explored the relationship of its expression with neuronal loss in the ganglion cell layer (GCL) in retinal ischemia/reperfusion. We found that MMP3 was widely expressed in many cells throughout the layers of the rat retinae, including Vertebrate neuron-specific nuclear protein (NeuN)-, parvalbumin-, calbindin-, protein kinase C-α-, glial fibrillary acidic protein-, glutamine synthetase- and CD11b-positive cells. Furthermore, all rats were treated with high intraocular pressure (HIOP) for 1 h (h) and sacrificed at 6 h, 1 day (d), 3 d, and 7 d after HIOP. Compared to the normal control, the expression of both proenzyme MMP3 and active MMP3 were significantly up-regulated after HIOP treatment without alteration of the laminar distribution pattern. Moreover, inhibiting MMP3 ameliorated the loss of NeuN-positive cells in the GCL following HIOP. In summary, our data demonstrates that MMP3 is expressed in multiple types of neurons and glial cells in normal rat retinae. Simultaneously, the up-regulation of its expression and activity are closely involved in neuronal loss in the GCL in retinal ischemia/reperfusion.
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Tu
authors.initials: T
authors.lastname: Hu
authors.affiliation: Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Qiuting
authors.initials: Q
authors.lastname: You
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Anesthesia, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Tong
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shang
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Ophthalmology, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan Province, People's Republic of China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, Hunan Province, People's Republic of China. zenglp0205@csu.edu.cn.
authors.forename: Leping
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, Hunan Province, People's Republic of China. huangjufang@csu.edu.cn.
authors.forename: Jufang
authors.initials: J
authors.lastname: Huang
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D007429:Intraocular Pressure; D007511:Ischemia / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology; D011498:Protein Precursors / Q000037:antagonists & inhibitors / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D012160:Retina / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000473:pathology; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D000081:Acetamides; D034961:Antigens, Nuclear; D061965:Matrix Metalloproteinase Inhibitors; C583689:NNGH compound; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; C546339:Rbfox3 protein, rat; D013449:Sulfonamides; D019278:Matrix Metalloproteinase 3
keywords: High intraocular pressure; Matrix metalloproteinase 3; N-Isobutyl-N-(4-methoxyphenylsulfonyl)glycyl-hydroxamic acid; Retinal ischemia/reperfusion
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