title: Transcriptome Analysis of Murine Olfactory Sensory Neurons during Development Using Single Cell RNA-Seq.
abstract: Mammalian odor reception is achieved by highly specialized olfactory sensory neurons (OSNs) located in the nasal cavity. Despite their importance for the daily survival of most mammals, the gene expression and regulatory profiles of these single neurons are poorly understood. Here, we report the isolation of individual GFP-labeled OSNs from Olfr73-GFP mice at different developmental stages followed by Next Generation Sequencing, thereby analyzing the detailed transcriptome for the first time. We characterized the repertoire of olfactory receptors (ORs) and found that in addition to the highly and predominant detectable Olfr73, 20 additional ORs were stably detectable at lower transcript levels in adult mice. Additionally, OSNs collected from mice of earlier developmental stages did not show any stable OR patterns. However, more than one predominant OR per OSN was detectable.
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Scholz
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kalbe
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Jansen
authors.affiliation: Cologne Center for Genomics (CCG), University of Cologne, 50931 Cologne, NRW, Germany and Institute of Human Genetics, University Hospital Cologne, 50931 Cologne, NRW, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Altmueller
authors.affiliation: Cologne Center for Genomics (CCG), University of Cologne, 50931 Cologne, NRW, Germany and.
authors.forename: Christian
authors.initials: C
authors.lastname: Becker
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Mohrhardt
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schreiner
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Guenter
authors.initials: G
authors.lastname: Gisselmann
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.
authors.forename: Hanns
authors.initials: H
authors.lastname: Hatt
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany, sabrina.osterloh@rub.de.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Osterloh
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis
chemical_list: D012313:RNA
keywords: development; olfaction; olfactory receptors; single cell; transcriptome analysis