title: Exercise modulates synaptic acetylcholinesterase at neuromuscular junctions.
abstract: Acetylcholinesterase plays a major role in neuromuscular transmission and is regulated by neuromuscular activity. Since fast-twitch motor units are recruited with increased motor demand, we examined acetylcholinesterase regulation in rat leg muscles following treadmill training. Total acetylcholinesterase and specifically the membrane-bound tetramer increased in exercised fast-, but not slow-twitch muscles, while other isoforms remained unchanged. Synaptic acetylcholinesterase increased markedly in neuromuscular junctions of trained fibers, without concomitant changes in synaptic acetylcholine receptor, thus elevating synaptic acetylcholinesterase/receptor ratios. Electron microscopy showed that acetylcholinesterase increased in postjunctional folds and primary cleft, where it was added adjacent to the postsynaptic muscle membrane. Thus, although the primary acetylcholinesterase at the neuromuscular junction is the collagen-tailed asymmetric isoform associated with synaptic basal lamina, physiological demands such as strenuous exercise, or potentially pathological conditions, can selectively recruit the membrane-bound acetylcholinesterase tetramer to the synapse for optimal synaptic transmission.
authors.affiliation: Department of Medical Neurobiology, Institute for Medical Research - Israel-Canada (IMRIC), Hebrew University-Hadassah Medical School, Jerusalem 9112102, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Blotnick
authors.affiliation: Department of Medical Neurobiology, Institute for Medical Research - Israel-Canada (IMRIC), Hebrew University-Hadassah Medical School, Jerusalem 9112102, Israel. Electronic address: lili.anglister@huji.ac.il.
authors.forename: L
authors.initials: L
authors.lastname: Anglister
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D018656:Muscle Fibers, Fast-Twitch / Q000201:enzymology*; D009469:Neuromuscular Junction / Q000201:enzymology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007527:Isoenzymes; D000110:Acetylcholinesterase
keywords: acetylcholine receptor; acetylcholinesterase molecular forms; fast-twitch; neuromuscular junction; treadmill