title: The effect of continuous ELF-MFs on the level of 5-HIAA in the raphe nucleus of the rat.
abstract: The aim of this study was to investigate the effect of continuous extremely low frequency magnetic fields (ELF-MFs) with a frequency of 10 Hz and an intensity of 690-720 μT on the level of 5-hydroxyindolacetic acid (5-HIAA) in adult male Wistar rats. A total of 24 adult Wistar male rats were used, and after exposure with an ELF-MF for 15 successive days, all rats in each test were anesthetized with chloral hydrate. Then, they were placed in a stereotaxic frame for surgery and a microdialysis process. Dialysate samples were analyzed to measure the amount of 5-HIAA by high performance liquid chromatography (HPLC) using electrochemical detection. Results showed that ELF-MF exposure for 15 days, 1 h daily, was not effective in altering the level of 5-HIAA. However, ELF-MF exposure for 15 days, 3 h daily, decreased the level of the 5-HIAA in the raphe nucleus. It can be concluded that ELF-MFs affect the serotonergic system and may be used to treat nervous system diseases. This study is an initial step towards helping cure depression using ELF-MFs.
authors.affiliation: Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran shahbazi24@yahoo.com shahbazi@med.mui.ac.ir.
authors.forename: Daryoush
authors.initials: D
authors.lastname: Shahbazi-Gahrouei
authors.affiliation: Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Shiri
authors.affiliation: Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hojjatollah
authors.initials: H
authors.lastname: Alaei
authors.affiliation: Department of Physiology, Pasteur Institute, Tehran, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Naghdi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D060526:Magnetic Fields; D008297:Male; D055442:Metabolome / Q000528:radiation effects; D011903:Raphe Nuclei / Q000378:metabolism* / Q000528:radiation effects*; D017208:Rats, Wistar; D012015:Reference Standards; D012701:Serotonin / Q000378:metabolism
chemical_list: D012701:Serotonin; D006897:Hydroxyindoleacetic Acid
keywords: 5-hydroxyindoleacetic acid; ELF-MF; HPLC; microdialysis; raphe nuclei
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