title: Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is a lethal liver malignancy worldwide. In this study, we reported that protein phosphatase magnesium-dependent 1δ (PPM1D) was highly expressed in the majority of HCC cases (approximately 59%) and significantly associated with high serum α-fetoprotein (AFP) level (P = 0.044). Kaplan- Meier and Cox regression data indicated that PPM1D overexpression was an independent predictor of HCCspecific overall survival (HR, 2.799; 95% CI, 1.346-5.818, P = 0.006). Overexpressing PPM1D promoted cell viability and invasion, whereas RNA interference-mediated knockdown of PPM1D inhibited proliferation, invasion, and migration of cultured HCC cells. In addition, PPM1D suppression by small interfering RNA decreased the tumorigenicity of HCC cells in vivo. Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target for HCC.
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China. michelle.xuzhi@hotmail.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xia
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Dongying
authors.initials: D
authors.lastname: Gu
authors.affiliation: Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Bian
authors.affiliation: Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Zijun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pathology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Nantong Tumor Hospital, Nantong, 226361, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nantong Tumor Hospital, Nantong, 226361, China.
authors.forename: Song
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Surgery, Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: Nikki Pui-Yue
authors.initials: NP
authors.lastname: Lee
authors.affiliation: Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China. jinfeichen@sohu.com.
authors.forename: Jinfei
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D011379:Prognosis; D000071636:Protein Phosphatase 2C / Q000097:blood*; D034741:RNA, Small Interfering; D016019:Survival Analysis; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D000509:alpha-Fetoproteins; C000607458:PPM1D protein, human; D000071636:Protein Phosphatase 2C
keywords: PPM1D; hepatocellular carcinoma; prognosis; target therapy
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