title: A Tie2-driven BAC-TRAP transgenic line for in vivo endothelial gene profiling.
abstract: Recent technological innovations including bacterial artificial chromosome-based translating ribosome affinity purification (BAC-TRAP) have greatly facilitated analysis of cell type-specific gene expression in vivo, especially in the nervous system. To better study endothelial gene expression in vivo, we have generated a BAC-TRAP transgenic mouse line where the L10a ribosomal subunit is tagged with EGFP and placed under the control of the endothelium-specific Tie2 (Tek) promoter. We show that transgene expression in this line is widely, but specifically, detected in endothelial cells in several brain regions throughout pre- and postnatal development, as well as in other organs. We also show that this line results in highly significant enrichment of endothelium-specific mRNAs from brain tissues at different stages. This BAC-TRAP line therefore provides a useful genetic tool for in vivo endothelial gene profiling under various developmental, physiological, and pathological conditions. genesis 54:136-145, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Neuroscience, Program in Genetics and Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin.|Department of and Neurology, Program in Genetics and Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin.
authors.forename: Devi
authors.initials: D
authors.lastname: Santhosh
authors.affiliation: Department of Neuroscience, Program in Genetics and Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics* / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D042787:Receptor, TIE-2 / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes
chemical_list: D049452:Green Fluorescent Proteins; D042787:Receptor, TIE-2; C079086:Tek protein, mouse
keywords: mammal; transcription; vasculature
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