title: Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis.
abstract: Because of self-renewal, strong proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, mesenchymal stem cells are suggested to be potentially therapeutic for liver fibrosis/cirrhosis. In this study, we evaluated the treatment effects of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) on mouse liver cirrhosis induced by carbon tetrachloride. Portal and tail vein transplantations were examined to evaluate the effects of different injection routes on the liver cirrhosis model at 21 days after transplantation. BM-MSCs transplantation reduced aspartate aminotransferase/alanine aminotransferase levels at 21 days after injection. Furthermore, BM-MSCs induced positive changes in serum bilirubin and albumin and downregulated expression of integrins (600- to 7000-fold), transforming growth factor, and procollagen-α1 compared with the control group. Interestingly, both injection routes ameliorated inflammation and liver cirrhosis scores. All mice in treatment groups had reduced inflammation scores and no cirrhosis. In conclusion, transplantation of BM-MSCs via tail or portal veins ameliorates liver cirrhosis in mice. Notably, there were no differences in treatment effects between tail and portal vein administrations. In consideration of safety, we suggest transfusion of bone marrow-derived mesenchymal stem cells via a peripheral vein as a potential method for liver fibrosis treatment.
authors.affiliation: Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam; Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam.
authors.forename: Nhung Hai
authors.initials: NH
authors.lastname: Truong
authors.affiliation: Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam.
authors.forename: Nam Hai
authors.initials: NH
authors.lastname: Nguyen
authors.affiliation: Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam.
authors.forename: Trinh Van
authors.initials: TV
authors.lastname: Le
authors.affiliation: Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam.
authors.forename: Ngoc Bich
authors.initials: NB
authors.lastname: Vu
authors.affiliation: University of Medicine and Pharmacy Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
authors.forename: Nghia
authors.initials: N
authors.lastname: Huynh
authors.affiliation: Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam.
authors.forename: Thanh Van
authors.initials: TV
authors.lastname: Nguyen
authors.affiliation: University of Medicine and Pharmacy Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
authors.forename: Huy Minh
authors.initials: HM
authors.lastname: Le
authors.affiliation: Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam; Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam.
authors.forename: Ngoc Kim
authors.initials: NK
authors.lastname: Phan
authors.affiliation: Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam; Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City 700000, Vietnam.
authors.forename: Phuc Van
authors.identifier: 0000-0001-7254-0717
authors.initials: PV
authors.lastname: Pham
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