title: Protective effect and mechanism of action of saponins isolated from the seeds of gac (Momordica cochinchinensis Spreng.) against cisplatin-induced damage in LLC-PK1 kidney cells.
abstract: This study was performed to investigate the renoprotective effect and mechanism of Momordicae Semen, gac seeds, against the cisplatin-induced damage in LLC-PK1 kidney cells. In order to identify the active components, three major saponins were isolated from extract of the gac seed, gypsogenin 3-O-β-d-galactopyranosyl(1→2)-[α-L-rhamnopyranosyl(1→3)]-β-d-glucuronopyranoside (1), quillaic acid 3-O-β-D-galactopyranosyl(1→2)-[α-L-rhamnopyranosyl(1→3)]-β-D-glucuronopyranoside (2), and momordica saponin I (3). Compounds 1 and 2 ameliorated cisplatin-induced nephrotoxicity up to 80% of the control value at both 5 and 25μM. Phosphorylation of MAPKs was decreased along cisplatin treatment after treatment with compounds 1 and 2. These results show that blocking the MAPKs signaling cascade plays a critical role in mediating the renoprotective effect of Momordicae Semen extract and compounds 1 and 2.
authors.affiliation: Institute of Pharmaceutical Sciences, College of Pharmacy, CHA University, Seongnam 13488, Republic of Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Jung
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea; College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
authors.forename: Dahae
authors.initials: D
authors.lastname: Lee
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
authors.forename: Jae Sik
authors.initials: JS
authors.lastname: Yu
authors.affiliation: Institute of Pharmaceutical Sciences, College of Pharmacy, CHA University, Seongnam 13488, Republic of Korea.
authors.forename: Hojin
authors.initials: H
authors.lastname: Namgung
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea. Electronic address: kkang@gachon.ac.kr.
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea. Electronic address: khkim83@skku.edu.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects* / Q000473:pathology*; D018374:LLC-PK1 Cells; D015394:Molecular Structure; D029775:Momordica / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D012503:Saponins; D002945:Cisplatin
keywords: Caspase-3; Cisplatin; MAPKs; Momordica cochinchinensis; Nephrotoxicity; Saponin