title: Role of long non-coding RNA MIAT in proliferation, apoptosis and migration of lens epithelial cells: a clinical and in vitro study.
abstract: Age-related cataract is among the most common chronic disorders of ageing and is the world's leading blinding disorder. Long non-coding RNAs play important roles in several biological processes and complicated diseases. However, the role of lncRNAs in the setting of cataract is still unknown. Here, we extracted total RNAs from the transparent and age-matched cataractous human lenses, and determined lncRNA expression profiles using microarray analysis. We found that 38 lncRNAs were differentially expressed between transparent and cataractous lenses. 17 of 20 differentially expressed lncRNAs were further verified by quantitative RT-PCRs. One top abundant lncRNA, MIAT, was specifically up-regulated both in the plasma fraction of whole blood and aqueous humor of cataract patients. MIAT knockdown could affect the proliferation, apoptosis and migration of Human lens epithelial cells (HLECs) upon oxidative stress. Posterior capsule opacification (PCO) is a common complication of cataract surgery, which is associated with abnormal production of inflammatory factors. MIAT knockdown could repress tumour necrosis factor-α-induced abnormal proliferation and migration of HLECs, suggesting a potential role of MIAT in PCO-related pathological process. Moreover, we found that MIAT acted as a ceRNA, and formed a feedback loop with Akt and miR-150-5p to regulate HLEC function. Collectively, this study provides a novel insight into the pathogenesis of age-related cataract.
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Ling-Feng
authors.initials: LF
authors.lastname: Dong
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Rong-Mei
authors.initials: RM
authors.lastname: Zhou
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yao
authors.affiliation: The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Song
authors.affiliation: The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
mesh_terms: D000368:Aged; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D002386:Cataract / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007908:Lens, Crystalline / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism; D008875:Middle Aged; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D062085:RNA, Long Noncoding; C533097:MIR150, human; C517383:Miat long non-coding RNA
keywords: cataract; cell migration; cell viability; circulating RNA; long non-coding RNA
references.citation: J Cell Mol Med. 2014 Apr;18(4):656-70
references.pmid: 24495224
references.citation: Invest Ophthalmol Vis Sci. 2014 Feb;55(2):941-51
references.pmid: 24436191
references.citation: Antioxid Redox Signal. 2014 Aug 10;21(5):700-4
references.pmid: 24527727
references.citation: Circ Res. 2015 Mar 27;116(7):1143-56
references.pmid: 25587098
references.citation: Exp Eye Res. 2015 Jul;136:100-15
references.pmid: 25783492
references.citation: Ann N Y Acad Sci. 2001 Sep;945:1-7
references.pmid: 11708462
references.citation: Invest Ophthalmol Vis Sci. 2002 Jan;43(1):198-204
references.pmid: 11773032
references.citation: J Mol Biol. 2004 Feb 27;336(4):971-86
references.pmid: 15095873
references.citation: Exp Eye Res. 2004 Jul;79(1):3-9
references.pmid: 15183095
references.citation: Exp Eye Res. 1979 May;28(5):601-10
references.pmid: 446578
references.citation: Surv Ophthalmol. 1995 Jan-Feb;39(4):323-34
references.pmid: 7725232
references.citation: EMBO J. 1998 Apr 1;17(7):1860-70
references.pmid: 9524110
references.citation: Invest Ophthalmol Vis Sci. 1998 Dec;39(13):2696-706
references.pmid: 9856780
references.citation: Exp Eye Res. 1998 Nov;67(5):577-85
references.pmid: 9878220
references.citation: Exp Eye Res. 2004 Dec;79(6):935-40
references.pmid: 15642332
references.citation: Eur J Ophthalmol. 2005 Mar-Apr;15(2):213-20
references.pmid: 15812762
references.citation: Mol Vis. 2006;12:342-9
references.pmid: 16636651
references.citation: FEBS Lett. 2007 Mar 6;581(5):795-9
references.pmid: 17289032
references.citation: Nat Rev Genet. 2011 Dec;12(12):861-74
references.pmid: 22094949
references.citation: Annu Rev Biochem. 2012;81:145-66
references.pmid: 22663078
references.citation: Neural Dev. 2011;6:32
references.pmid: 21936910
references.citation: Trends Cell Biol. 2011 Jun;21(6):354-61
references.pmid: 21550244
references.citation: Nat Rev Cancer. 2011 Jun;11(6):426-37
references.pmid: 21562580
references.citation: Philos Trans R Soc Lond B Biol Sci. 2011 Apr 27;366(1568):1278-92
references.pmid: 21402586
references.citation: Invest Ophthalmol Vis Sci. 2011 Jan;52(1):249-55
references.pmid: 20720227
references.citation: J Pathol. 2010 Jan;220(2):126-39
references.pmid: 19882673
references.citation: Genes Dev. 2009 Jul 1;23(13):1494-504
references.pmid: 19571179
references.citation: Exp Eye Res. 2009 Feb;88(2):257-69
references.pmid: 19013456
references.citation: Pathology. 2007 Apr;39(2):197-207
references.pmid: 17454749
references.citation: Prog Retin Eye Res. 2007 May;26(3):239-62
references.pmid: 17321191
references.citation: Invest Ophthalmol Vis Sci. 2013 Jan;54(1):323-32
references.pmid: 23221074
references.citation: Exp Eye Res. 2013 Mar;108:68-71
references.pmid: 23146683
references.citation: Mol Biol Rep. 2014 Jul;41(7):4493-505
references.pmid: 24623407