title: Histopathological and Biochemical Effects of Ecballium elaterium on Sepsis-Induced Lung Injury.
abstract: OBJECTIVE
  The aim of this study was to investigate the role of Ecballium elaterium (EE) on sepsis-induced lung injury.
  
  
  METHODS
  A total of 30 male rats were divided into three groups as follows: control, sepsis, and treatment groups (sepsis + EE) with each group containing 10 rats. A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. In the treatment group, rats were injected intraperitoneally with 2.5 mg/kg EE after CLP. Interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values after a 24-hr period were measured via cardiac puncture. Animals were harvested after the procedure and biochemical analysis was done and histopathological changes of the tissue sections of lungs were examined thereafter.
  
  
  RESULTS
  A statistically significant decrease was observed in the IL-6 (p < .05), TNF-α (p < .05), and TOS (p < .01) levels in the sera of the treatment group compared to those of the sepsis group. Following the treatment, the TOS (p = .01) and OSI (p < .05) levels in the lung tissue of rats indicated a statistically significant decrease compared to those of the sepsis group. The histopathological follow-up undertaken after the administration of the EE treatment to septic rats showed significantly lower values of alveolar wall thickness (p < .001), interstitial edema (p = .018), and neutrophil infiltration (p = .047).
  
  
  CONCLUSIONS
  EE treatment may have beneficial effects on sepsis-induced lung injury, and therefore has potential for clinical use.
authors.affiliation: a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Melike
authors.initials: M
authors.lastname: Demir
authors.affiliation: a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Mahsuk
authors.initials: M
authors.lastname: Taylan
authors.affiliation: a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Halide
authors.initials: H
authors.lastname: Kaya
authors.affiliation: b Department of Biochemistry , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Ekinci
authors.affiliation: c Department of Neurology , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Arslan
authors.affiliation: d Department of Infectious Diseases , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Aslan
authors.affiliation: e Department of Pathology , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Aysenur
authors.initials: A
authors.lastname: Keles
authors.affiliation: a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Sureyya
authors.initials: S
authors.lastname: Yılmaz
authors.affiliation: a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
authors.forename: Cengizhan
authors.initials: C
authors.lastname: Sezgi
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D019662:Cucurbitaceae; D004195:Disease Models, Animal; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
keywords: Ecballium elaterium; lung; rat; sepsis; total antioxidant status; total oxidant status