title: Rapid high mass resolution mass spectrometry using matrix-assisted ionization.
abstract: Matrix-assisted ionization (MAI) is demonstrated to be a robust and sensitive analytical method capable of analyzing proteins such as cholera toxin B-subunit and pertussis toxin mutant from conditions containing relatively high amounts of inorganic salts, buffers, and preservatives without the need for prior sample clean-up or concentration. By circumventing some of the sample preparation steps, MAI simplifies and accelerates the analytical workflow for biological samples in complex media. The benefits of multiply charged ions characteristic of electrospray ionization (ESI) and the robustness of matrix-assisted laser desorption/ionization (MALDI) can be obtained from a single method, making it well suited for analysis of proteins and other biomolecules at ultra-high resolution as demonstrated on an Orbitrap Fusion where protein subunits were resolved for which MALDI-time-of-flight failed. MAI results are compared with those obtained with ESI, MALDI, and laserspray ionization methods and fundamental commonalities discussed.
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA; Cardiovascular Research Institute, Wayne State University School of Medicine, Detroit, MI 48202, USA. Electronic address: strimpin@chem.wayne.edu.
authors.forename: Sarah
authors.initials: S
authors.lastname: Trimpin
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Shameemah
authors.initials: S
authors.lastname: Thawoos
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Casey D
authors.initials: CD
authors.lastname: Foley
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Woodall
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Ellen D
authors.initials: ED
authors.lastname: Inutan
authors.affiliation: Proteomics Core Facility, Institute of Environmental Health Sciences, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Stemmer
mesh_terms: D007477:Ions / Q000737:chemistry; D007834:Lasers; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D007477:Ions; D011506:Proteins
keywords: High resolution; Ionization; Laserspray; Mass spectrometry; Matrix-assisted; Protein
references.citation: J Proteome Res. 2015 Nov 6;14 (11):4662-73
references.pmid: 26390080
references.citation: Nat Biotechnol. 1999 Jul;17(7):676-82
references.pmid: 10404161
references.citation: Anal Chem. 2010 Jun 15;82(12):4998-5001
references.pmid: 20469839
references.citation: Nat Rev Drug Discov. 2006 Dec;5(12):993-6
references.pmid: 17139284
references.citation: Mol Cell Proteomics. 2013 Mar;12(3):792-6
references.pmid: 23242551
references.citation: J Am Soc Mass Spectrom. 1998 Jan;9(1):1-7
references.pmid: 9679590
references.citation: Biomed Environ Mass Spectrom. 1988 Sep;17(3):229-37
references.pmid: 3214677
references.citation: Anal Chem. 2001 Nov 1;73(21):5005-14
references.pmid: 11721892
references.citation: Mol Cell Proteomics. 2010 Feb;9(2):362-7
references.pmid: 19955086
references.citation: Anal Bioanal Chem. 2015 Apr;407(10):2777-89
references.pmid: 25694144
references.citation: Anal Chem. 2007 Jan 1;79(1):71-8
references.pmid: 17194123
references.citation: Anal Chem. 2010 Jan 1;82(1):11-5
references.pmid: 19904915
references.citation: Anal Chem. 2011 Jul 15;83(14):5469-75
references.pmid: 21678939
references.citation: Anal Chem. 2015 Aug 18;87(16):8360-6
references.pmid: 26176279
references.citation: J Am Soc Mass Spectrom. 2016 Jan;27(1):4-21
references.pmid: 26486514
references.citation: Anal Chem. 2013 Feb 19;85(4):2005-9
references.pmid: 23373868
references.citation: Anal Chem. 2015;87(9):4667-74
references.pmid: 25756217
references.citation: Lab Chip. 2007 Nov;7(11):1394-412
references.pmid: 17960264
references.citation: Science. 1989 Oct 27;246(4929):497-500
references.pmid: 2683073
references.citation: Rapid Commun Mass Spectrom. 2015 Nov 15;29(21):1929-37
references.pmid: 26443390
references.citation: J Am Soc Mass Spectrom. 2013 May;24(5):722-32
references.pmid: 23526166
references.citation: Anal Chem. 1990 May 1;62(9):882-99
references.pmid: 2194402
references.citation: Nat Biotechnol. 2000 Jan;18(1):104-5
references.pmid: 10625404
references.citation: J Am Soc Mass Spectrom. 2012 Oct;23(10):1644-60
references.pmid: 22791582
references.citation: J Am Soc Mass Spectrom. 1999 Jul;10(7):625-34
references.pmid: 10384726