title: Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System.
abstract: The production of recombinant proteins using the baculovirus expression vector system (BEVS) in large-scale agitated bioreactors is discussed in this chapter. Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, methods involved with advanced on-line monitoring and bioreactor control, which have a significant impact on the overall process success, are briefly discussed.
authors.affiliation: Department of Chemical Engineering, Ecole Polytechnique de Montreal, 2500 Chemin de Polytechnique, Montreal, Canada.|National Research Council Canada, Montreal, Canada.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Thompson
authors.affiliation: National Research Council Canada, Montreal, Canada.
authors.forename: Johnny
authors.initials: J
authors.lastname: Montes
authors.affiliation: Department of Chemical Engineering, University of Waterloo, 200 University Ave W., Waterloo, ON, Canada, N2L 3G1. marc.aucoin@uwaterloo.ca.
authors.forename: Marc G
authors.initials: MG
authors.lastname: Aucoin
authors.affiliation: National Research Council Canada, Montreal, Canada.|Department of Bioengineering, McGill University, Montreal, Canada.
authors.forename: Amine A
authors.initials: AA
authors.lastname: Kamen
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D019149:Bioreactors / Q000821:virology*; D002452:Cell Count; D049109:Cell Proliferation; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D009038:Motion; D010100:Oxygen / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D061987:Sf9 Cells; D018411:Spodoptera; D013242:Sterilization
chemical_list: D011994:Recombinant Proteins; D010100:Oxygen
keywords: Baculovirus; Bioreactor; Fed-batch; High cell density; Insect cell; Large-scale; Lytic system; On-line monitoring; Recombinant protein expression; Serum-free medium