title: [USE OF LASER RAMAN-LUMINESCENT TECHNOLOGIES FOR EVALUATION OF QUALITY OF MEAT PRODUCTS AND DETERMINATION OF THE DEGREE OF THEIR BACTERIAL CONTAMINATION].
abstract: OBJECTIVE
  Determination of the effect of microorganisms on spoilage of meat products during various temperature regimes of storage by integral indexes of luminescent lines in their spectra and development of an algorithm of microorganism indication by an express method using laser Raman-luminescent spectroscopy.
  
  
  METHODS
  Minced meat from beef and pork was used. Determination of quantity of mesophilic aerobic and opportunistic-anaerobic microorganisms was carried out by serial 10-fold dilutions with subsequent parallel seeding into Rida count total 24 plates and Petri dishes with 5% blood agar. Sample study was carried out in luminescent software-hardware complex Enspectr L405 (a variant of Enspectr M software-hardware complexes).
  
  
  RESULTS
  Meat spoilage was established to be caused to a large degree by Pseudomonas genus (P. fluorescens, P. putida, P. fragi et al.) bacterial growth. Raman-luminescent spectra of bacteria that compose microflora, characterizing and accompanying beef and pork spoilage, were measured and recorded into a database.
  
  
  CONCLUSIONS
  The results obtained will allow to use this technique in the future for both express-indication and differentiation of microorganisms and express-evaluation of quality of meat products at all stages of their manufacturing, storage, transport and realization.
authors.forename: V I
authors.initials: VI
authors.lastname: Kukushkin
authors.forename: E V
authors.initials: EV
authors.lastname: Satusheva
authors.forename: M T
authors.initials: MT
authors.lastname: Aleksandrov
authors.forename: O A
authors.initials: OA
authors.lastname: Morozova
authors.forename: E P
authors.initials: EP
authors.lastname: Pashkov
authors.forename: O A
authors.initials: OA
authors.lastname: Ambartsumyan
authors.forename: V A
authors.initials: VA
authors.lastname: Amosova
mesh_terms: D000818:Animals; D002417:Cattle; D005516:Food Microbiology; D006801:Humans; D007834:Lasers; D008163:Luminescent Measurements; D011549:Pseudomonas / Q000302:isolation & purification* / Q000472:pathogenicity; D000069466:Red Meat / Q000382:microbiology*; D013059:Spectrum Analysis, Raman; D013552:Swine; D013696:Temperature