title: Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA.
abstract: In this study, new and feasible UV-visible spectrophotometric and multivariate spectrophotometric methods were described for the simultaneous determination of hydrochlorothiazide (HCTZ), hydralazine hydrochloride (H.HCl), and reserpine (RES) in combined pharmaceutical tablets. Methanol was used as a solvent for analysis and the whole UV region was scanned from 200-400 nm. The resolution was obtained by using multivariate methods such as the net analyte signal method (NAS), principal component analysis (PCA), and net analyte signal-principal component analysis (NAS-PCA) applied to the UV spectra of the mixture. The results obtained from all of the three methods were compared. NAS-PCA showed a lot of resolved data as compared to NAS and PCA. Thus, the NAS-PCA technique is a combination of NAS and PCA methods which is advantageous to obtain the information from overlapping results.
authors.affiliation: Department of Pharmaceutical Analysis, Indo-Soviet Friendship College of Pharmacy (ISFCP), Moga 142001, India.
authors.forename: Chetan
authors.initials: C
authors.lastname: Sharma
authors.affiliation: Department of Pharmaceutical Analysis, Indo-Soviet Friendship College of Pharmacy (ISFCP), Moga 142001, India.
authors.forename: Pragya Nand
authors.initials: PN
authors.lastname: Badyal
authors.affiliation: Department of Pharmaceutical Analysis, Indo-Soviet Friendship College of Pharmacy (ISFCP), Moga 142001, India.
authors.forename: Ravindra K
authors.initials: RK
authors.lastname: Rawal
keywords: Net Analyte Signal; Principal Component Analysis; Relative Standard Error of Prediction
references.citation: Talanta. 2014 Jun;123:186-99
references.pmid: 24725882
references.citation: J Pharm Sci. 1978 May;67(5):650-3
references.pmid: 641802
references.citation: Anal Chem. 1972 Mar;44(3):565-70
references.pmid: 5014738
references.citation: Yao Xue Xue Bao. 2004 Aug;39(8):618-20
references.pmid: 15563064
references.citation: Indian J Pharm Sci. 2012 Jan;74(1):18-23
references.pmid: 23204617