title: Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex.
abstract: Tandem repetitive DNA is highly abundant in eukaryotic genomes and contributes to transcription control and genome stability. However, how the individual sequences within tandem repeats behave remains largely unknown. Here we develop a collection of fission yeast strains with a reporter gene inserted at different units in a tandem repeat array. We show that, contrary to what is usually assumed, transcriptional silencing and replication timing among the individual repeats differ significantly. RNAi-mediated H3K9 methylation is essential for the silencing position effect. A short hairpin RNA of ura4(+) induces silencing in trans within the tandem array in a position-dependent manner. Importantly, the position effect depends on the condensin subunit, cut3(+). Cut3 promotes the position effect via interaction with the RNA-induced transcriptional silencing (RITS) complex. This study reveals variations in silencing within tandem DNA repeats and provides mechanistic insights into how DNA repeats at the individual level are regulated.
authors.affiliation: Department of Biology, New York University, New York, NY 10003-6688, USA.
authors.forename: Haijin
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Biology, New York University, New York, NY 10003-6688, USA.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Biology, New York University, New York, NY 10003-6688, USA.
authors.forename: Danni
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Biology, New York University, New York, NY 10003-6688, USA.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hochwagen
authors.affiliation: Department of Biology, New York University, New York, NY 10003-6688, USA. Electronic address: fl43@nyu.edu.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D042522:DNA Replication Timing; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017930:Genes, Reporter; D006657:Histones / Q000378:metabolism; D008745:Methylation; D046912:Multiprotein Complexes / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D034622:RNA Interference; D034743:RNA-Induced Silencing Complex / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D046912:Multiprotein Complexes; D021122:Protein Subunits; D034743:RNA-Induced Silencing Complex; D029702:Schizosaccharomyces pombe Proteins; C108312:condensin complexes; D000251:Adenosine Triphosphatases
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