title: Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
abstract: Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid. Attenuated strains of SG are proven and widely used vaccine candidates because of advantages like induction of strong humoral and cell-mediated immune responses. In the present study, we investigated the interaction of chicken bone marrow-derived dendritic cells (chBM-DCs) with an attenuated SG (JOL1355) strain that secretes a heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated and cultured in the presence of recombinant chicken GM-CSF and IL-4 cytokines. The chBM-DCs were infected with JOL1355 at an multiplicity of infection of 10. JOL1355 was able to invade dendritic cells (DCs); however, the survival of JOL1355 in DCs decreased over time. At 24 h post infection, IL-6, IL-10 and IFN-γ transcript levels were significantly increased in JOL1355-infected DCs compared to non-stimulated DCs. Flow cytometry analysis showed an increased proportion of cells producing CD40, CD80, and MHC class II in the JOL1355-infected cultures compared to the non-stimulated control. In addition, JOL1355-stimulated chBM-DCs could induce significant expression of IL-2 in co-culture with autologous CD4+ T cells. Based on these results, we conclude that chBM-DCs are capable of internalizing the live attenuated SG vaccine candidate and the infected chBM-DCs show signs of maturation as evidenced by the upregulated expression of costimulatory molecules and cytokines.
authors.affiliation: a Department of Veterinary Public Health, College of Veterinary Medicine , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Nitin Machindra
authors.initials: NM
authors.lastname: Kamble
authors.affiliation: a Department of Veterinary Public Health, College of Veterinary Medicine , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Chetan Vilas
authors.initials: CV
authors.lastname: Jawale
authors.affiliation: a Department of Veterinary Public Health, College of Veterinary Medicine , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: John Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D007111:Immunity, Cellular; D011201:Poultry Diseases / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D019779:Salmonella enterica / Q000276:immunology*; D014613:Vaccines, Attenuated
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D004768:Enterotoxins; D014613:Vaccines, Attenuated
keywords: CD4+ T cells; Salmonella Gallinarum; costimulatory molecules; cytokines; dendritic cells; fowl typhoid