title: [A study involving antioxidizability and cytotoxicity of two kinds of phenol from Ajania Salicifolia and their mechanisms of apoptosis].
abstract: OBJECTIVE
  To extract two kinds of phenols 4-hydroxy-3, 5-dimethoxy-4-(2-oxopropyl) cyclohexa-2, 5-dien-l-one and 6-methoxy-5,7-dihydroxy coumarin (named as I and H compounds respectively) from Ajania salicifolia and to investigate their antioxidation and cytotoxicity to tumors and explore their pro-apoptosis mechanism.
  
  
  METHODS
  The antioxidant activities of two compounds were assessed by ABTS and DPPH radical-scavenging assays. Two compounds were evaluated for their cytotoxicity against human chronic myelogenous leukemia (K562) cells using the MIT assay. The expression of NF-kappaB P65 mRNA in K562 apoptotic cells was measured by reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative PCR. In addition, protein expression levels of the NF-ICB P65, p-Akt, Fas, P-catenina and E-cadherin were also measured by Western blot.
  
  
  RESULTS
  (1) We found that compound I displayed significant inoxidizability, while compound II had no obvious antioxidizability. (2) In cytotoxicity experiments, compound I didn't display cytotoxicity while compound H displayed obvious cytotoxicity. (3) Compared with the blank group, the expression of NF-kappaB P65 mRNA in K562 cell after treatment with compound II was obviously up-regulated. (4) Compared with the blank group, the expression levels of NF-kappaB P65, Fas, beta-catenina and E-cadherin were significantly increased in compound II treated groups and it appeared obvious dose-effect relationship between the expression of protein and drug concentration.
  
  
  CONCLUSIONS
  Two phenols have obvious antioxidizability and cytotoxicity respectively. On the one hand, the tumor-suppressing mechanism of compound II maybe act by up-regulation the expression of NF-kappaB P65 and Fas protein; thereby, affecting the classical Fas apoptosis signaling pathways. On the other hand, it can also up-regulate the expression of protein beta-catenin and E-cadherin, which participate in the adhesion between cells, and accordingly, playing an important role in preventing the proliferation and metastasis of cancer cells.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hong-ru
authors.initials: HR
authors.lastname: Wu
authors.forename: Qiang-kun
authors.initials: QK
authors.lastname: Liang
authors.forename: Yun-xia
authors.initials: YX
authors.lastname: Li
authors.forename: Yan-yu
authors.initials: YY
authors.lastname: Lu
authors.forename: Yao
authors.initials: Y
authors.lastname: Long
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hong-fang
authors.initials: HF
authors.lastname: Li
mesh_terms: D015703:Antigens, CD; D017209:Apoptosis; D019659:Asteraceae / Q000737:chemistry*; D015820:Cadherins / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D051058:Oncogene Protein v-akt / Q000378:metabolism; D010636:Phenols / Q000737:chemistry*; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism; D015854:Up-Regulation; D051176:beta Catenin / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; C491901:FAS protein, human; D010636:Phenols; C498143:RELA protein, human; D051996:Transcription Factor RelA; D051176:beta Catenin; D019014:fas Receptor; D051058:Oncogene Protein v-akt