title: Improved Automated Radiosynthesis of [(11)C]PBR28.
abstract: Microglial activation is commonly identified by elevated levels of the 18 kDa translocator protein (TSPO) in response to several inflammatory processes. [(11)C]PBR28 is one of the most promising PET tracers to image TSPO in both human and non-human primates. In this study, we optimized the radiolabeling procedure of [(11)C]PBR28 for higher radiochemical yield, radiochemical purity, and specific activity, which can be easily translated to any automated module for clinical trials. Time-activity curves (TACs) derived from the dynamic PET imaging of male rhesus monkey brains demonstrated that [(11)C]PBR28 had suitable kinetics with radiotracer accumulation observed in the caudate, putamen, cerebellum, and frontal cortex region.
authors.affiliation: Department of Radiology, Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157, USA.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Solingapuram Sai
authors.affiliation: Department of Radiology, Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157, USA.
authors.forename: Don
authors.initials: D
authors.lastname: Gage
authors.affiliation: Department of Physiology and Pharmacology, Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Nader
authors.affiliation: Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Mach
authors.affiliation: Department of Radiology, Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157, USA.
authors.forename: Akiva
authors.initials: A
authors.lastname: Mintz
keywords: Microglial activation; Positron Emission Tomography (PET); Synthesis; TSPO
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