title: Giardia duodenalis and Giardia enterica in children: first evidence of assemblages A and B in Eastern Slovakia.
abstract: For an overview on the occurrence of Giardia assemblages in children in Eastern Slovakia, we examined 259 faecal samples of children from the segregated settlement in Medzev, 30 samples of children from the orphanage in Medzev and 40 samples of children with autism from the Special Elementary School in Košice. Thirty-eight samples (14.67 %) from the segregated settlement, 19 samples (63.33 %) from the orphanage and two samples (5.0 %) from the Special Elementary School were positive for Giardia by flotation. The initial microscopic diagnostics were completed by the genotyping of the triosephosphate isomerase-gene loci (tpi genes) which revealed the existence of two Giardia assemblages in Slovak population, namely Giardia duodenalis (assemblage A) and Giardia enterica (assemblage B). These results represent the first evidence of A and B assemblages in children in Slovakia. Epidemiological significance and the impact on the public health of Giardia infection are highlighted.
authors.affiliation: Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovak Republic.
authors.forename: G
authors.initials: G
authors.lastname: Štrkolcová
authors.affiliation: Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovak Republic. goldova@uvlf.sk.
authors.forename: M
authors.initials: M
authors.lastname: Goldová
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Maďar
authors.affiliation: Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, Šrobárova 2, Košice, 041 80, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Čechová
authors.affiliation: Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, Šrobárova 2, Košice, 041 80, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Halánová
authors.affiliation: Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Mojžišová
mesh_terms: D000818:Animals; D002648:Child; D005243:Feces / Q000469:parasitology; D005838:Genotype; D005872:Giardia / Q000145:classification* / Q000235:genetics; D005873:Giardiasis / Q000453:epidemiology* / Q000469:parasitology*; D006801:Humans; D018154:Slovakia / Q000453:epidemiology
keywords: Assemblages A, B; Children; Giardia duodenalis; Giardia enterica; Slovakia
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