title: Presence of neural progenitors in spontaneous canine gliomas: A histopathological and immunohistochemical study of 20 cases.
abstract: Gliomas are the most common primary brain tumours in humans and are associated with a poor prognosis. An accurate animal model of human glioma tumorigenesis is needed to test new treatment strategies. Dogs represent a promising model because they develop spontaneous diffusely-infiltrating gliomas. This study investigated whether spontaneous canine gliomas contain cancer stem cells previously identified in all grades of human gliomas. Twenty spontaneous cases of canine gliomas were graded according to the human WHO classification. The expression of different markers of lineage differentiation was evaluated with immunohistochemistry as follows: nestin and CD133 for neural stem cells, doublecortin for neuronal progenitor cells, Olig2 for glial progenitor cells, glial fibrillary acidic protein, vimentin and S-100 for mature glial cells, and NeuN and βIII-tubulin for mature neurons. Gliomas were characterised as follows: five grade II (oligodendrogliomas); nine grade III (seven anaplastic oligodendrogliomas, one anaplastic astrocytoma, one anaplastic oligoastrocytoma); six grade IV (glioblastomas). Immunohistochemical evaluation revealed that (1) nestin and CD133 were expressed in all grades of gliomas with a higher proportion of positive cells in high-grade gliomas; (2) the expression of S-100 protein and Olig2 did not differ substantially between astrocytic and oligodendroglial tumours, and (3) all gliomas were negative for mature neuron markers. The results demonstrated the presence of undifferentiated neural progenitors in all grades of spontaneous canine gliomas, confirming the relevance of this animal model for further studies on cancer stem cells.
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain. Electronic address: francisco.fernandez.flores@uab.cat.
authors.forename: Francisco
authors.initials: F
authors.lastname: Fernández
authors.affiliation: Université de Toulouse, INP, ENVT, Unité d'Anatomie - Imagerie - Embryologie, F-31076 Toulouse, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Cérébrale et Handicaps Neurologiques UMR 825, CHU Purpan, Toulouse, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Deviers
authors.affiliation: Laboratoire d'Anatomie Pathologique Vétérinaire du Sud-Ouest (LAPVSO), F-31201 Toulouse, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Dally
authors.affiliation: Université de Toulouse, INP, ENVT, Unité d'Anatomie - Imagerie - Embryologie, F-31076 Toulouse, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Cérébrale et Handicaps Neurologiques UMR 825, CHU Purpan, Toulouse, France.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mogicato
authors.affiliation: Université de Toulouse, INP, ENVT, Unité d'Anatomie Pathologique, F-31076 Toulouse, France; INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Maxence
authors.initials: M
authors.lastname: Delverdier
authors.affiliation: CHV Frégis, F-94110 Arcueil, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Cauzinille
authors.affiliation: Clinique Advetia, F-75012 Paris, France.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Gnirs
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain; Neurology-Neurosurgery Service of the Veterinary Teaching Hospital of the Universitat Autònoma de Barcelona (UAB-FHCV), Barcelona, Spain.
authors.forename: Sònia
authors.initials: S
authors.lastname: Añor
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain; Neurology-Neurosurgery Service of the Veterinary Teaching Hospital of the Universitat Autònoma de Barcelona (UAB-FHCV), Barcelona, Spain.
authors.forename: Cristian
authors.initials: C
authors.lastname: de la Fuente
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
authors.forename: Dolors
authors.initials: D
authors.lastname: Fondevila
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Martí
authors.initials: M
authors.lastname: Pumarola
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000503:physiopathology / Q000662:veterinary*; D019070:Cell Lineage; D004283:Dog Diseases / Q000209:etiology* / Q000503:physiopathology; D004285:Dogs; D005260:Female; D005910:Glioma / Q000209:etiology / Q000503:physiopathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D060787:Neoplasm Grading / Q000662:veterinary; D014411:Neoplastic Stem Cells / Q000145:classification / Q000502:physiology*
keywords: Cancer stem cells; Canine; Glioma; Histopathology; Immunohistochemistry