title: A SNP in pri-miR-10a is associated with recurrent spontaneous abortion in a Han-Chinese population.
abstract: MicroRNA-10a (miR-10a) has a wide range of functions in nearly all mammalian tissues and is involved in the occurrence of many diseases. However, it remains unknown whether miR-10a is associated with human recurrent spontaneous abortion (RSA). In this study, we found that rs3809783 A > T in miR-10a coding region was significantly associated with the increase of the risk of human unexplained RSA (URSA) acquisition in a Han-Chinese population. The T allele of rs3809783 hindered the production of mature miR-10a. A to T substitution in miR-10a rs3809783 repressed cell proliferation and migratory capacity. Further investigation discovered that Bcl-2-interacting mediator (Bim) was the functional target of miR-10a and inversely regulated Bim expression. Dual-luciferase assay indicated that A allele in miR-10a rs3809783 could more effectively suppress Bim expression than T allele. In addition, A to T substitution in miR-10a rs3809783 attenuated the sensibility of cells to progesterone and its antagonist mifepristone. Collectively, our data suggest that rs3809783 A > T in pri-miR-10a may be conductive to the genetic predisposition to RSA by disrupting the production of mature miR-10a and reinforcing the expression of Bim.
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Lv
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Su
authors.affiliation: Haidian Maternal & Child Health Hospital, Beijing, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Tian
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Liu
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.|Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology* / Q000235:genetics*; D017209:Apoptosis; D000072224:Bcl-2-Like Protein 11 / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis*; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002681:China / Q000453:epidemiology; D005260:Female; D005434:Flow Cytometry; D020022:Genetic Predisposition to Disease; D006801:Humans; D007124:Immunoenzyme Techniques; D035683:MicroRNAs / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000072224:Bcl-2-Like Protein 11; D015415:Biomarkers; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: haplotype; miR-10a; recurrent spontaneous abortion; rs3809783
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