title: Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis.
abstract: BACKGROUND
  Adenomyosis (AM) has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs) of AM.
  
  
  METHODS
  Uterine tissue samples from 22 patients (cases) with histologically conﬁrmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K + channel (BKCa)-α/β subunits, voltage-gated potassium channel (Kv) 4.2, and Kv4.3. Student's t-test was used to compare the expression.
  
  
  RESULTS
  The BKCa-α/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-α messenger RNA (mRNA) (0.62 ± 0.19-fold decrease, P < 0.05) and Kv4.3 mRNA (0.67 ± 0.20-fold decrease, P < 0.05) decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA.
  
  
  CONCLUSIONS
  The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Leng
authors.forename: Jing-He
authors.initials: JH
authors.lastname: Lang
mesh_terms: D062788:Adenomyosis / Q000378:metabolism*; D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D051672:Shal Potassium Channels / Q000378:metabolism; D014590:Uterine Contraction / Q000502:physiology; D014594:Uterine Neoplasms / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels
references.citation: Hum Reprod. 2000 Jun;15 Suppl 1:81-9
references.pmid: 10928421
references.citation: Biomech Model Mechanobiol. 2015 Jun;14(3):437-44
references.pmid: 25217062
references.citation: Exp Physiol. 2001 Mar;86(2):255-64
references.pmid: 11429642
references.citation: Fertil Steril. 2002 Jun;77(6):1156-61
references.pmid: 12057721
references.citation: Reprod Biol Endocrinol. 2004 Sep 17;2:66
references.pmid: 15373944
references.citation: Science. 1997 May 23;276(5316):1268-72
references.pmid: 9157888
references.citation: Am J Physiol Endocrinol Metab. 2005 Feb;288(2):E335-41
references.pmid: 15454398
references.citation: Semin Cell Dev Biol. 2007 Jun;18(3):332-9
references.pmid: 17596977
references.citation: Reprod Biol Endocrinol. 2007;5:41
references.pmid: 17980032
references.citation: Magn Reson Imaging Clin N Am. 2008 Nov;16(4):673-84, ix
references.pmid: 18926430
references.citation: Reprod Biol Endocrinol. 2009;7:27
references.pmid: 19344525
references.citation: Arch Gynecol Obstet. 2009 Oct;280(4):529-38
references.pmid: 19644696
references.citation: Methods Mol Biol. 2009;590:295-306
references.pmid: 19763512
references.citation: Fertil Steril. 2010 May 1;93(7):2130-6
references.pmid: 19268938
references.citation: AJR Am J Roentgenol. 2011 May;196(5):1206-13
references.pmid: 21512093
references.citation: Fertil Steril. 2013 Jan;99(1):231-40
references.pmid: 22999795
references.citation: J Physiol Pharmacol. 2013 Dec;64(6):795-805
references.pmid: 24388895
references.citation: Endocrinology. 2014 Aug;155(8):3112-22
references.pmid: 24914944
references.citation: Arch Gynecol Obstet. 2015 Apr;291(4):917-32
references.pmid: 25241270
references.citation: Biol Reprod. 2001 May;64(5):1526-34
references.pmid: 11319161