title: Two genes substitute for the mouse Y chromosome for spermatogenesis and reproduction.
abstract: The mammalian Y chromosome is considered a symbol of maleness, as it encodes a gene driving male sex determination, Sry, as well as a battery of other genes important for male reproduction. We previously demonstrated in the mouse that successful assisted reproduction can be achieved when the Y gene contribution is limited to only two genes, Sry and spermatogonial proliferation factor Eif2s3y. Here, we replaced Sry by transgenic activation of its downstream target Sox9, and Eif2s3y, by transgenic overexpression of its X chromosome-encoded homolog Eif2s3x. The resulting males with no Y chromosome genes produced haploid male gametes and sired offspring after assisted reproduction. Our findings support the existence of functional redundancy between the Y chromosome genes and their homologs encoded on other chromosomes.
authors.affiliation: Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822, USA.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822, USA.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Riel
authors.affiliation: Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822, USA.
authors.forename: Victor A
authors.initials: VA
authors.lastname: Ruthig
authors.affiliation: Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822, USA.
authors.forename: Eglė A
authors.initials: EA
authors.lastname: Ortega
authors.affiliation: Aix-Marseille Université, INSERM, GMGF UMR_S 910, 13385 Marseille, France.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Mitchell
authors.affiliation: Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822, USA. mward@hawaii.edu.
authors.forename: Monika A
authors.initials: MA
authors.lastname: Ward
mesh_terms: D000818:Animals; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D006238:Haploidy; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D027724:Reproductive Techniques, Assisted; D055758:SOX9 Transcription Factor / Q000235:genetics*; D051876:Sex-Determining Region Y Protein / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D014960:X Chromosome / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D015852:Eukaryotic Initiation Factor-2; D055758:SOX9 Transcription Factor; D051876:Sex-Determining Region Y Protein; C527933:Sox9 protein, mouse; C497746:Sry protein, mouse
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