title: Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression.
abstract: The lepidopteran insect cells used with the baculovirus expression vector system (BEVS) are capable of synthesizing and accurately processing foreign proteins. However, proteins expressed in baculovirus-infected cells often fail to be completely processed, or are not processed in a manner that meets a researcher's needs. This chapter discusses a metabolic engineering approach that addresses this problem. Basically, this approach involves the addition of new or enhancement of existing protein processing functions in established lepidopteran insect cell lines. In addition to improvements in protein processing, this approach has also been used to improve protein expression levels obtained with the BEVS. Methods for engineering cell lines and assessing their properties as improved hosts for the BEVS are detailed. Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation, folding/trafficking, and expression are described in detail.
authors.affiliation: Invasive Insect Biocontrol & Behavior Laboratory, USDA, ARS, BARC, Building 007, Room 301, BARC-W, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA. Robert.L.Harrison@ars.usda.gov.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Harrison
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, WY, USA.
authors.forename: Donald L
authors.initials: DL
authors.lastname: Jarvis
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006031:Glycosylation; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology* / Q000821:virology; D014170:Transformation, Genetic
chemical_list: D011994:Recombinant Proteins
keywords: BEVS; Baculovirus; Baculovirus expression vector system; Cell transformation; Genetic engineering; Glycosyltransferase; Insect cells; Metabolic engineering; Protein N-glycosylation; Protein folding; Protein trafficking