title: Matrix Metallopeptidase 14 Plays an Important Role in Regulating Tumorigenic Gene Expression and Invasion Ability of HeLa Cells.
abstract: OBJECTIVE
  The objectives of this study were to investigate the functional effect of matrix metallopeptidase 14 (MMP14) on cell invasion in cervical cancer cells (HeLa line) and to study the underlying molecular mechanisms.
  
  
  METHODS
  Expression vector of short hairpin RNA targeting MMP14 was treated in HeLa cells, and then, transfection efficiency was verified by a florescence microscope. Transwell assay was used to investigate cell invasion ability in HeLa cells. Quantitative polymerase chain reaction and Western blotting analysis were used to detect the expression of MMP14 and relative factors in messenger RNA and protein levels, respectively.
  
  
  RESULTS
  Matrix metallopeptidase 14 short hairpin RNA expression vector transfection obviously decreased MMP14 expression in messenger RNA and protein levels. Down-regulation of MMP14 suppressed invasion ability of HeLa cells and reduced transforming growth factor β1 and vascular endothelial growth factor B expressions. Furthermore, MMP14 knockdown decreased bone sialoprotein and enhanced forkhead box protein L2 expression in both RNA and protein levels.
  
  
  CONCLUSIONS
  Matrix metallopeptidase 14 plays an important role in regulating invasion of HeLa cells. Matrix metallopeptidase 14 knockdown contributes to attenuating the malignant phenotype of cervical cancer cell.
authors.affiliation: *Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, China; and †Department of Biochemical and Molecular of Medical College, Soochow University, Suzhou, China.
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Zhang
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Han-Xi
authors.initials: HX
authors.lastname: Zheng
authors.forename: Lan
authors.initials: L
authors.lastname: Xu
authors.forename: You-Guo
authors.initials: YG
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*; D042561:Vascular Endothelial Growth Factor B / Q000235:genetics / Q000378:metabolism*
chemical_list: C421604:FOXL2 protein, human; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C506895:SPP1 protein, human; D053773:Transforming Growth Factor beta1; C467485:VEGFB protein, human; D042561:Vascular Endothelial Growth Factor B; D053495:Osteopontin; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14