title: Determination of chloramphenicol, thiamphenicol and florfenicol in milk and honey using modified QuEChERS extraction coupled with polymeric monolith-based capillary liquid chromatography tandem mass spectrometry.
abstract: A poly(lauryl methacrylate-co-methacrylic acid-co-ethylene glycol dimethacrylate) [LMA-MAA-EDMA] monolithic column was used to simultaneously determine amphenicol antibiotics (chloramphenicol/CAP, thiamphenicol/TAP, and florfenicol/FF) in milk and honey samples by capillary liquid chromatography tandem mass spectrometry (LC-MS/MS). QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was optimized for sample pretreatment. Good linearity (0.1-15 ng g(-1)) and extraction recoveries (95.8-100.2% and 95.6-99.3% for milk and honey samples, respectively; n=3) with minor matrix effect (≦ 5% ion suppression) were obtained. Limits of detection were estimated at 0.02-0.045 ng g(-1). Good intra-day/inter-day precision (0.2-9.1%/0.3-8.7%) and accuracy (90.5-110.0%/93.4-109.3%) were achieved. With more than 200 analyses of real samples, no noticeable carry-over and deterioration of separation efficiency were observed using the monolithic column. The applicability of the developed QuEChERS-capillary LC-MS/MS method was demonstrated by determining the occurrence of CAP, TAP, and FF in various milk and honey samples.
authors.affiliation: Department of Chemistry, Soochow University, 70 Linhsi Road, Shihlin, 11102 Taipei, Taiwan.
authors.forename: Hsiang-Yu
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Soochow University, 70 Linhsi Road, Shihlin, 11102 Taipei, Taiwan.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Soochow University, 70 Linhsi Road, Shihlin, 11102 Taipei, Taiwan. Electronic address: msfuh@scu.edu.tw.
authors.forename: Ming-Ren
authors.initials: MR
authors.lastname: Fuh
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002701:Chloramphenicol / Q000032:analysis / Q000302:isolation & purification; D002853:Chromatography, Liquid; D003365:Costs and Cost Analysis; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D059022:Food Safety; D006722:Honey / Q000032:analysis*; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012449:Safety; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D011108:Polymers; D002701:Chloramphenicol; C035534:florfenicol; D013839:Thiamphenicol
keywords: Amphenicol antibiotics; Polymeric monolith-based capillary liquid chromatography; QuEChERS (quick; Tandem mass spectrometry (MS/MS); and safe) extraction; cheap; easy; effective; rugged