title: Novel multiresidue method for determination of pesticides in red wine using gas chromatography-mass spectrometry and solid phase extraction.
abstract: A new multiresidue method was developed for determination of 25 pesticide residues in red wine by gas chromatography coupled to mass spectrometry with a single run of 23.63 min. Samples were extracted from wine with solid phase extraction using Oasis HLB. Mixture of methanol and water was used for rinsing, while acetonitrile and n-hexane were used as elution solvents. Method was validated according to SANCO/12571/2013 criteria in wide linearity range (limit of quantification - 400 μg L(-1)). Limits of quantification (LOQ) were well below 10 μg L(-1) for most pesticides and recoveries at 2×LOQ and 10×LOQ concentration levels were in range 70-120%. Precision, expressed as a relative standard deviation, was always under 14%. The method was applied to 32 red wine samples from Croatia. Pesticides were detected in 30 samples with a total of 15 pesticides found, 7 of which were at a high concentration.
authors.affiliation: Croatian Centre for Agriculture, Food and Rural Affairs, Institute for Plant Protection, Rim 98, 10000 Zagreb, Croatia. Electronic address: maja.pelajic@hcphs.hr.
authors.forename: Maja
authors.initials: M
authors.lastname: Pelajić
authors.affiliation: Ministry of Agriculture, Phytosanitary Sector, Department of Plant Protection Products, Grada Vukovara 68, 10000 Zagreb, Croatia.
authors.forename: Gorana
authors.initials: G
authors.lastname: Peček
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Mutavdžić Pavlović
authors.affiliation: Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia.
authors.forename: Dubravka
authors.initials: D
authors.lastname: Vitali Čepo
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010575:Pesticides / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D014920:Wine / Q000032:analysis* / Q000469:parasitology*
chemical_list: D010575:Pesticides
keywords: GC–MS; Multiresidue method; Pesticide residues; Red wine; Solid phase extraction