title: Regulation of ZAT12 protein stability: The role of hydrogen peroxide.
abstract: Signaling mediated by reactive oxygen species (ROS) has emerged as a key component of plants' responses to environmental stress. The ROS-regulated transcription factor ZAT12 was revealed as a negative regulator of iron (Fe) deficiency responses through its direct interaction with the bHLH protein FIT. In the epidermis of the early root differentiation zone, ZAT12 stability depended on the presence of the ZAT12 EAR motif. It was concluded that ZAT12 may be the target of 2 alternative degradation pathways. Here, we present a model aiming to explain the regulatory mechanisms by which ZAT12 could be targeted for degradation and to predict the types of potential regulators involved. In addition to an E3 ubiquitin ligase, we predict 2 critical regulatory factors, namely a protein interacting with the ZAT12 EAR motif and a ROS-responsive regulatory protein.
authors.affiliation: a Institute of Botany, Heinrich-Heine University , Düsseldorf , Germany.
authors.forename: Tzvetina
authors.initials: T
authors.lastname: Brumbarova
authors.affiliation: b Department of Plant Genetics and Breeding, Faculty of Agronomy , Vietnam National University of Agriculture , Trauquy, Gialam , Hanoi , Vietnam.
authors.forename: Cham Thi Tuyet
authors.initials: CT
authors.lastname: Le
authors.affiliation: a Institute of Botany, Heinrich-Heine University , Düsseldorf , Germany.
authors.forename: Rumen
authors.initials: R
authors.lastname: Ivanov
authors.affiliation: a Institute of Botany, Heinrich-Heine University , Düsseldorf , Germany.|c Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine University , Düsseldorf , Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Bauer
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008954:Models, Biological; D055550:Protein Stability; D059748:Proteolysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C502133:ZAT12 protein, Arabidopsis; D006861:Hydrogen Peroxide
keywords: FIT; Fe acquisition; ROS signaling; ZAT12; hydrogen peroxide
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