title: The prognostic implication of SRSF2 mutations in Chinese patients with acute myeloid leukemia.
abstract: Recently, somatic mutations in SRSF2 gene have been discovered in a proportion of hematologic malignancies including acute myeloid leukemia (AML). This study was aimed to investigate SRSF2 mutations in Chinese AML patients. High-resolution melting analysis (HRMA) was developed to screen SRSF2 mutations in 249 cases with AML, and then direct DNA sequencing was used to verify the results of HRMA. In this study, 3.6 % (9/249) of Chinese AML patients were found with heterozygous SRSF2 mutations. Patients with SRSF2 mutations were older than those with wild-type SRSF2 (P = 0.014). No differences in the sex, blood parameters, French-American-British classification (FAB) subtypes, and karyotypes were observed between AML patients with and without SRSF2 mutations. Although the overall survival (OS) of SRSF2-mutated patients was inferior to those without mutations in both whole AML patients (median 4 vs. 11 months, respectively; P = 0.006) and cytogenetically normal patients (median 2 vs. 12 months, respectively; P = 0.008), multiple analysis disclosed that SRSF2 mutation was not an independent prognostic factor in AML patients. These results suggest that SRSF2 mutation occurs at a low frequency in aged AML patients and might not be associated with adverse prognosis in Chinese AML patients.
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Jing
authors.identifier: http://orcid.org/0000-0001-5855-7379
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Yao
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Ma
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Xiang-Mei
authors.initials: XM
authors.lastname: Wen
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Gao-Fei
authors.initials: GF
authors.lastname: Xiao
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qian
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China. linjiangmail@sina.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd., Zhenjiang, Jiangsu, 212002, People's Republic of China. qianjun0007@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002681:China / Q000453:epidemiology; D005260:Female; D006579:Heterozygote; D006801:Humans; D053208:Kaplan-Meier Estimate; D015470:Leukemia, Myeloid, Acute / Q000208:ethnology / Q000235:genetics* / Q000401:mortality; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis; D000068103:Serine-Arginine Splicing Factors / Q000235:genetics*; D016037:Single-Blind Method; D055815:Young Adult
chemical_list: D009363:Neoplasm Proteins; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors
keywords: Acute myeloid leukemia; High-resolution melting analysis; Prognosis; SRSF2 mutation
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