title: The Chlamydia trachomatis Protease CPAF Contains a Cryptic PDZ-Like Domain with Similarity to Human Cell Polarity and Tight Junction PDZ-Containing Proteins.
abstract: The need for more effective anti-chlamydial therapeutics has sparked research efforts geared toward further understanding chlamydial pathogenesis mechanisms. Recent studies have implicated the secreted chlamydial serine protease, chlamydial protease-like activity factor (CPAF) as potentially important for chlamydial pathogenesis. By mechanisms that remain to be elucidated, CPAF is directed to a discrete group of substrates, which are subsequently cleaved or degraded. While inspecting the previously solved CPAF crystal structure, we discovered that CPAF contains a cryptic N-terminal PSD95 Dlg ZO-1 (PDZ) domain spanning residues 106-212 (CPAF106-212). This PDZ domain is unique in that it bears minimal sequence similarity to canonical PDZ-forming sequences and displays little sequence and structural similarity to known chlamydial PDZ domains. We show that the CPAF106-212 sequence is homologous to PDZ domains of human tight junction proteins.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Maksimchuk
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina, United States of America.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Alser
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina, United States of America.
authors.forename: Rui
authors.initials: R
authors.lastname: Mou
authors.affiliation: Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, United States of America.
authors.forename: Raphael H
authors.initials: RH
authors.lastname: Valdivia
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, United States of America.|Department of Chemistry, Duke University, Durham, North Carolina, United States of America.
authors.forename: Dewey G
authors.initials: DG
authors.lastname: McCafferty
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D016764:Cell Polarity; D002692:Chlamydia trachomatis / Q000201:enzymology* / Q000235:genetics; D019295:Computational Biology; D017124:Conserved Sequence; D016680:Genome, Bacterial; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D040681:Structural Homology, Protein; D013329:Structure-Activity Relationship; D019108:Tight Junctions / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010447:Peptide Hydrolases
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