title: Initial research on the relationship between let-7 family members in the serum and massive cerebral infarction.
abstract: Eighty-eight ischemic stroke patients with massive cerebral infarction (MCI) who met our selection criteria were included in this study. MCI was assessed using the Glasgow Coma Scale (GCS) at hospital admission and at 2 weeks. The sera of all patients and controls were sampled at 48 h after the patients' attacks, and the sera of patients with MCI who had no severe cardiopulmonary complications, including those with hemorrhagic transformation (HT), were sampled again at 2 weeks. The relative expression of let-7 miRNA in the serum was determined by real-time qRT-PCR, and the blood levels of lipids, glucose, high-sensitivity C-reactive protein (hs-CRP), homocysteine and blood pressure were measured at admission. Interleukin-6 (IL-6) levels were detected by ELISA, and a luciferase assay was performed to confirm that IL-6 was a gene target of let-7. The relative expression of let-7f was significantly down-regulated in MCI without HT patients compared with controls (P<0.001), and it was positively correlated with GCS (P<0.01) and negatively correlated with hs-CRP (P<0.01). The relative expression of let-7f was significantly up-regulated in MCI patients with HT (P<0.01). IL-6 is a direct target gene for let-7f, and IL-6 expression was increased in MCI without HT patients compared to controls (P<0.01). The expression of let-7f in serum is associated with MCI without HT, which specifically inhibits IL-6. This suggests that let-7f may control inflammation in patients with MCI without HT.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China; Key-Disciplines Laboratory Clinical-Medicine Henan, Zhengzhou, Henan, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China; Key-Disciplines Laboratory Clinical-Medicine Henan, Zhengzhou, Henan, China.
authors.forename: Shaoyun
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Guan
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Clinical Medcine, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Peidong
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Teng
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. Electronic address: realptdq@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Neurology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. Electronic address: jiayanjie1971@aliyun.com.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Jia
mesh_terms: D001786:Blood Glucose / Q000378:metabolism; D002544:Cerebral Infarction / Q000097:blood*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020521:Stroke / Q000097:blood*; D015854:Up-Regulation
chemical_list: D001786:Blood Glucose; D015850:Interleukin-6; D008055:Lipids; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human
keywords: Hemorrhagic transformation; IL-6; Inflammation; Let-7; Massive cerebral infarction; hs-CRP