title: Impacts of ABCB1 (G1199A) polymorphism on resistance, uptake, and efflux to steroid drugs.
abstract: 1. P-glycoprotein (P-gp) substrates, including steroid drugs, involve in the inter-individual differences in resistant phenotype. This study was performed to evaluate whether G1199A polymorphism in ABCB1 gene can alter the sensitivity, accumulation, and transepithelial efflux to steroids in LLC-PK1 cells. 2. The stable recombinant LLC-PK1 cell lines transfected with ABCB1 1199G and ABCB1 1199A were used to assess the sensitivity, accumulation, and transepithelial permeability to steroids. 3. The cells transfected with 1199A allele displayed stronger resistance to aldosterone, dexamethasone, and cortisol (2.5-, 2.0-, and 1.6-fold, respectively) than cells overexpressing 1199G allele, while the two types of recombinant cells showed a similar resistance to corticosterone. The accumulation of aldosterone, dexamethasone, and cortisol in recombinant 1199A cells were significantly decreased when compared to 1199G cells (2.9-, 4.4-, and 3.9-fold, respectively). The net efflux ratios of P-gp-mediated aldosterone, dexamethasone, and cortisol in cells expressing 1199A allele were apparently greater than cells transfected with 1199G allele (3.3-, 3.5-, and 4.0-fold, respectively). 4. The impacts of ABCB1 (G1199A) single nucleotide polymorphism on the efflux of P-gp substrates presented as drug-specific. Overall, the transport ability of P-gp-dependent steroid drugs in recombinant model overexpressing variant 1199A allele is stronger in comparison to cells overexpressing wild-type 1199G allele. Therefore, the ABCB1 (G1199A) polymorphism may affect effective steroids concentration in target cells by regulating the drug transport and distribution.
authors.affiliation: a Department of Pharmacy , Renmin Hospital of Wuhan University , Wuhan , China.
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.affiliation: a Department of Pharmacy , Renmin Hospital of Wuhan University , Wuhan , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Department of Pharmacy , Renmin Hospital of Wuhan University , Wuhan , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Department of Pharmacy , Renmin Hospital of Wuhan University , Wuhan , China.
authors.forename: Dan-Yun
authors.initials: DY
authors.lastname: Wei
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004351:Drug Resistance / Q000235:genetics*; D018374:LLC-PK1 Cells; D020641:Polymorphism, Single Nucleotide; D013256:Steroids / Q000378:metabolism*; D013552:Swine
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D013256:Steroids
keywords: ABCB1 (G1199A); accumulation; polymorphism; resistance; steroids; transepithelial permeability