title: [EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING].
abstract: OBJECTIVE
  Study the effect of lactobacilli exopolysaccharides (EPS)on cytokine and phagocyte activity in vitro and in mice organism during modelling of an infectious process.
  
  
  METHODS
  Lactobacillus delbrueckii subsp. delbrueckii B-1596 (laksaran 1596), L. delbrueckii B-1936 (laksaran 1936) and L. delbrueckii ssp. bulgaricus (laksaran Z) were used in the study. EPS were administered into white mice 1 hour after the Staphylococcus aureus 209-P infection. Index of phagocyte completion and index of killing activation (IKA) were calculated during phagocyte activity study. IL-1α, TNF-α, IFN-γ and IL-4 cytokine content was determined in blood sera and macrophage supernatants.
  
  
  RESULTS
  Laksaran 1596, 1936 and Z had ambiguous effect on cytokine production. Laksaran: Z and 1936, 6 hours after mice infection increased IL-1 content in blood sera. Laksaran Z had the most pronounced effect on macrophages, resulting in an increase of active macrophages, facilitating increased digestion of S. aureus 209-P and IKA increase, stimulated cytokine production.
  
  
  CONCLUSIONS
  The results obtained allow to speak about a possibility of using laksaran Z as a prophylaxis immune modulating preparation for correction of animal cytokine status.
authors.forename: E A
authors.initials: EA
authors.lastname: Gorelnikova
authors.forename: L V
authors.initials: LV
authors.lastname: Karpunina
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007375:Interleukin-1 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007778:Lactobacillus / Q000737:chemistry / Q000276:immunology; D051379:Mice; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D013203:Staphylococcal Infections / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D007375:Interleukin-1; D011135:Polysaccharides, Bacterial; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma