title: Gonadectomy reduces the density of androgen receptor-immunoreactive neurons in male rat's hippocampus: testosterone replacement compensates it.
abstract: BACKGROUND
  In the present study, the role of gonadectomy on memory impairment and the density of androgen receptor-immunoreactive neurons in rats' hippocampus as well as the ability of testosterone to compensate of memory and the density of androgen receptors in the hippocampus was evaluated.
  
  
  METHODS
  Adult male rats (except intact-no testosterone group) were bilaterally castrated, and behavioral tests performed 2 weeks later. Animals bilaterally cannulated into lateral ventricles and then received testosterone (10, 40 and 120 µg/0.5 µl DMSO) or vehicle (DMSO; 0.5 µl) for gonadectomized-vehicle group, 30 min before training in water maze test. The androgen receptor-immunoreactive neurons were detected by immunohistochemical technique in the hippocampal areas.
  
  
  RESULTS
  In the gonadectomized male rats, a memory deficit was found in Morris water maze test on test day (5th day) after DMSO administration. Gonadectomy decreased density of androgen receptor-immunoreactive neurons in the rats' hippocampus. The treatment with testosterone daily for 5 days attenuated memory deficits induced by gonadectomy. Testosterone also significantly increased the density of androgen receptor-immunoreactive neurons in the hippocampal areas. The intermediate dose of this hormone (40 µg) appeared to have a significant effect on spatial memory and the density of androgen receptor-immunoreactive neurons in gonadectomized rats' hippocampus.
  
  
  CONCLUSIONS
  The present study suggests that testosterone can compensate memory failure in gonadectomized rats. Also testosterone replacement can compensate the reduction of androgen receptor-immunoreactive neurons density in the rats' hippocampus after gonadectomy.
authors.affiliation: Neuroscience Research Center, Department of Anatomy, Faculty of Medicine, Golestan University of Medical Sciences, Km 4 Gorgan-Sari Road (Shastcola), P.O. Box 4934174515, Gorgan, Iran. sajad.moghadami@gmial.com.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Moghadami
authors.affiliation: Neuroscience Research Center, Department of Anatomy, Faculty of Medicine, Golestan University of Medical Sciences, Km 4 Gorgan-Sari Road (Shastcola), P.O. Box 4934174515, Gorgan, Iran. mejahanshahi@yahoo.com.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Jahanshahi
authors.affiliation: Neuroscience Research Center, Department of Physiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. hamsep49@yahoo.com.
authors.forename: Hamid
authors.initials: H
authors.lastname: Sepehri
authors.affiliation: Neuroscience Research Center, Department of Pharmacology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. haminhplc@yahoo.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Amini
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D011944:Receptors, Androgen; D013739:Testosterone
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