title: Effects of Bauhinia forficata Tea on Oxidative Stress and Liver Damage in Diabetic Mice.
abstract: This study was designed to evaluate the effects of Bauhinia forficata Link subsp. pruinosa (BF) tea on oxidative stress and liver damage in streptozotocin (STZ)-induced diabetic mice. Diabetic male mice have remained 30 days without any treatment. BF treatment started on day 31 and continued for 21 days as a drinking-water substitute. We evaluated (1) BF chemical composition; (2) glucose levels; (3) liver/body weight ratio and liver transaminases; (4) reactive oxygen species (ROS), lipid peroxidation, and protein carbonylation in liver; (5) superoxide dismutase (SOD) and catalase (CAT) activities in liver; (6) δ-aminolevulinate dehydratase (δ-ALA-D) and nonprotein thiols (NPSH) in liver; (7) Nrf2, NQO-1, and HSP70 levels in liver and pancreas. Phytochemical analyses identified four phenols compounds. Diabetic mice present high levels of NQO-1 in pancreas, increased levels of ROS and lipid peroxidation in liver, and decrease in CAT activity. BF treatment normalized all these parameters. BF did not normalize hyperglycemia, liver/body weight ratio, aspartate aminotransferase, protein carbonyl, NPSH levels, and δ-ALA-D activity. The raised oxidative stress seems to be a potential mechanism involved in liver damage in hyperglycemic conditions. Our results indicated that BF protective effect could be attributed to its antioxidant capacity, more than a hypoglycemic potential.
authors.affiliation: Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Andréia Caroline Fernandes
authors.initials: AC
authors.lastname: Salgueiro
authors.affiliation: Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Vanderlei
authors.identifier: 0000-0001-6940-9080
authors.initials: V
authors.lastname: Folmer
authors.affiliation: Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Marianne Pires
authors.initials: MP
authors.lastname: da Silva
authors.affiliation: Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Andreas Sebastian Loureiro
authors.initials: AS
authors.lastname: Mendez
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, Campus São Gabriel, Avenida Antônio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Ana Paula Pegoraro
authors.initials: AP
authors.lastname: Zemolin
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, Campus São Gabriel, Avenida Antônio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Thaís
authors.initials: T
authors.lastname: Posser
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, Campus São Gabriel, Avenida Antônio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Jeferson Luis
authors.identifier: 0000-0002-3874-8618
authors.initials: JL
authors.lastname: Franco
authors.affiliation: Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Robson Luiz
authors.initials: RL
authors.lastname: Puntel
authors.affiliation: Departamento de Morfologia, Universidade Federal de Santa Maria, Avenida Roraima 1000, Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Gustavo Orione
authors.initials: GO
authors.lastname: Puntel
mesh_terms: D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000201:enzymology / Q000473:pathology*; D018840:HSP70 Heat-Shock Proteins; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000069459:Teas, Herbal / Q000633:toxicity; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D018840:HSP70 Heat-Shock Proteins; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D000069459:Teas, Herbal; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; D000623:Porphobilinogen Synthase
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