title: Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
abstract: The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from "Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET" by Peinado and colleagues, published in Nature Medicine in 2012 (Peinado et al., 2012). The key experiments being replicated are from Figures 4E, as well as Supplementary Figures 1C and 5A. In these experiments, Peinado and colleagues show tumor exosomes enhance metastasis to bones and lungs, which is diminished by reducing Met expression in exosomes (Peinado et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published in eLife.
authors.affiliation: System Biosciences, LLC, Mountain View, United States.
authors.forename: Jake
authors.initials: J
authors.lastname: Lesnik
authors.affiliation: System Biosciences, LLC, Mountain View, United States.
authors.forename: Travis
authors.initials: T
authors.lastname: Antes
authors.affiliation: Transgenic Research Center, Stanford University School of Medicine, Stanford, United States.
authors.forename: Jeewon
authors.initials: J
authors.lastname: Kim
authors.affiliation: University of Virginia, Charlottesville, United States.
authors.forename: Erin
authors.initials: E
authors.lastname: Griner
authors.affiliation: University of Cambridge, Cambridge, United Kingdom.
authors.forename: Luisa
authors.initials: L
authors.lastname: Pedro
mesh_terms: D019046:Bone Marrow Neoplasms / Q000556:secondary*; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D008545:Melanoma / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000473:pathology*; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology*
keywords: MET; Reproducibility Project: Cancer Biology; exosomes; human biology; medicine; metastasis; methodology; mouse; receptor tyrosine kinase
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