title: Screen-printed digital microfluidics combined with surface acoustic wave nebulization for hydrogen-deuterium exchange measurements.
abstract: An inexpensive digital microfluidic (DMF) chip was fabricated by screen-printing electrodes on a sheet of polyimide. This device was manually integrated with surface acoustic wave nebulization (SAWN) MS to conduct hydrogen/deuterium exchange (HDX) of peptides. The HDX experiment was performed by DMF mixing of one aqueous droplet of angiotensin II with a second containing various concentrations of D2O. Subsequently, the degree of HDX was measured immediately by SAWN-MS. As expected for a small peptide, the isotopically resolved mass spectrum for angiotensin revealed that maximum deuterium exchange was achieved using 50% D2O. Additionally, using SAWN-MS alone, the global HDX kinetics of ubiquitin were found to be similar to published NMR data and back exchange rates for the uncooled apparatus using high inlet capillary temperatures was less than 6%.
authors.affiliation: University of Washington, Seattle, WA, USA. Electronic address: lucasm7@uw.edu.
authors.forename: Lucas
authors.initials: L
authors.lastname: Monkkonen
authors.affiliation: University of Washington, Seattle, WA, USA.
authors.forename: J Scott
authors.initials: JS
authors.lastname: Edgar
authors.affiliation: Trioptics GmbH, Wedel, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Winters
authors.affiliation: Deurion, LLC, Seattle, WA, USA; University of Maryland, Baltimore, MD, USA.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Heron
authors.affiliation: University of Edinburgh, Edinburgh, UK.
authors.forename: C Logan
authors.initials: CL
authors.lastname: Mackay
authors.affiliation: University Grenoble-Alpes, Grenoble, France; CEA, iRTSV, Biologie à Grande Echelle, Grenoble, France; INSERM, U1038 Grenoble, France.
authors.forename: Christophe D
authors.initials: CD
authors.lastname: Masselon
authors.affiliation: University of Edinburgh, Edinburgh, UK.
authors.forename: Adam A
authors.initials: AA
authors.lastname: Stokes
authors.affiliation: University of Edinburgh, Edinburgh, UK.
authors.forename: Patrick R R
authors.initials: PRR
authors.lastname: Langridge-Smith
authors.affiliation: Deurion, LLC, Seattle, WA, USA; University of Maryland, Baltimore, MD, USA. Electronic address: dgoodlett@rx.umaryland.edu.
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
mesh_terms: D000809:Angiotensins / Q000737:chemistry; D041961:Deuterium Exchange Measurement; D007700:Kinetics; D013058:Mass Spectrometry; D046210:Microfluidic Analytical Techniques; D010455:Peptides / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D000809:Angiotensins; D010455:Peptides; D025801:Ubiquitin
keywords: Hydrogen-deuterium exchange; Mass spectrometry; Screen-printed digital microfluidics; Surface acoustic wave nebulization