title: [Exploring the active ingredient of Chinese yellow wine which could inhibit the Hcy induced proliferation and migration of vascular smooth muscle cells].
abstract: OBJECTIVE
  To explore the active ingredients in the Chinese yellow wine could inhibit the proliferation and migration of rat vascular smooth muscle cells induced by homocysteine (Hcy).
  
  
  METHODS
  The primary culture and identification of rat vascular smooth muscle cells (VSMCs) was conducted, and the VSMCs in passage 4-7 were used in the following experiments. The VSMCs were divided into 7 groups: control, Hcy (1 mmol/L), Hcy + oligosaccharide, Hcy + polypeptides, Hcy + polyphenols, Hcy + alcohol, Hcy + Chinese yellow wine and were given the corresponding treatment. The proliferation of VSMCs was determined by MTT. Transwell chambers and would healing were employed to test the migratory ability of VSMCs. Wester blot and gelatin zymography were used to investigate the expressions and activities of metal matrix proteinase 2/9 (MMP-2/9) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in VSMCs of each group.
  
  
  RESULTS
  Compared with control group, the proliferation, migration and the expression and activity of MMP-2/9 of VSMCs were significantly increased in the VSMCs of Hcy group (P < 0.01). Compared with Hcy group, the proliferation, migration and the expression and activity of MMP-2/9 of VSMCs were significantly decreases in the VSMCs of polypeptides group, polyphenols group and Chinese yellow wine group. However, the expression of TIMP-2 among each group had no significant difference.
  
  
  CONCLUSIONS
  Polypeptides and polyphenols in the Chinese yellow wine could inhibit the proliferation and migration of VSMCs induced by Hcy.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Meng
authors.forename: Chang-zuan
authors.initials: CZ
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Xiao-ya
authors.initials: XY
authors.lastname: Zhai
authors.forename: Cheng-jian
authors.initials: CJ
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Ju-fang
authors.initials: JF
authors.lastname: Chi
authors.forename: Hang-yuan
authors.initials: HY
authors.lastname: Guo
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006710:Homocysteine; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D010455:Peptides / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D051381:Rats; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D014920:Wine
chemical_list: D010455:Peptides; D059808:Polyphenols; D006710:Homocysteine; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat