title: Hanseniaspora uvarum from Winemaking Environments Show Spatial and Temporal Genetic Clustering.
abstract: Hanseniaspora uvarum is one of the most abundant yeast species found on grapes and in grape must, at least before the onset of alcoholic fermentation (AF) which is usually performed by Saccharomyces species. The aim of this study was to characterize the genetic and phenotypic variability within the H. uvarum species. One hundred and fifteen strains isolated from winemaking environments in different geographical origins were analyzed using 11 microsatellite markers and a subset of 47 strains were analyzed by AFLP. H. uvarum isolates clustered mainly on the basis of their geographical localization as revealed by microsatellites. In addition, a strong clustering based on year of isolation was evidenced, indicating that the genetic diversity of H. uvarum isolates was related to both spatial and temporal variations. Conversely, clustering analysis based on AFLP data provided a different picture with groups showing no particular characteristics, but provided higher strain discrimination. This result indicated that AFLP approaches are inadequate to establish the genetic relationship between individuals, but allowed good strain discrimination. At the phenotypic level, several extracellular enzymatic activities of enological relevance (pectinase, chitinase, protease, β-glucosidase) were measured but showed low diversity. The impact of environmental factors of enological interest (temperature, anaerobia, and copper addition) on growth was also assessed and showed poor variation. Altogether, this work provided both new analytical tool (microsatellites) and new insights into the genetic and phenotypic diversity of H. uvarum, a yeast species that has previously been identified as a potential candidate for co-inoculation in grape must, but whose intraspecific variability had never been fully assessed.
authors.affiliation: Unité de recherche Œnologie, Institut de la Science de la Vigne et du Vin, University BordeauxVillenave d'Ornon, France; ENSCBP, Bordeaux INPPessac, France.
authors.forename: Warren
authors.initials: W
authors.lastname: Albertin
authors.affiliation: Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University Matieland, South Africa.
authors.forename: Mathabatha E
authors.initials: ME
authors.lastname: Setati
authors.affiliation: Unité de recherche Œnologie, Institut de la Science de la Vigne et du Vin, University BordeauxVillenave d'Ornon, France; Institut National de la Recherche Agronomique, Institut de la Science de la Vigne et du Vin, USC 1366 Institut National de la Recherche AgronomiqueVillenave d'Ornon, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Miot-Sertier
authors.affiliation: Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University Matieland, South Africa.
authors.forename: Talitha T
authors.initials: TT
authors.lastname: Mostert
authors.affiliation: Centre de Recherche Pernod Ricard Créteil, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Colonna-Ceccaldi
authors.affiliation: Biolaffort Bordeaux, France.
authors.forename: Joana
authors.initials: J
authors.lastname: Coulon
authors.affiliation: Centre de Recherche Pernod Ricard Créteil, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Girard
authors.affiliation: Biolaffort Bordeaux, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Moine
authors.affiliation: Institut National de la Recherche Agronomique, UMR Biodiversité Gènes et Ecosystèmes, PlateForme Génomique Cestas, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Pillet
authors.affiliation: Institut National de la Recherche Agronomique, UMR Biodiversité Gènes et Ecosystèmes, PlateForme Génomique Cestas, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Salin
authors.affiliation: Unité de recherche Œnologie, Institut de la Science de la Vigne et du Vin, University Bordeaux Villenave d'Ornon, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Bely
authors.affiliation: Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University Matieland, South Africa.
authors.forename: Benoit
authors.initials: B
authors.lastname: Divol
authors.affiliation: Unité de recherche Œnologie, Institut de la Science de la Vigne et du Vin, University BordeauxVillenave d'Ornon, France; Bordeaux Sciences AgroGradignan, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Masneuf-Pomarede
keywords: Hanseniaspora uvarum; intraspecific diversity; microsatellites; phenotypic screening; wine
references.citation: Food Microbiol. 2010 Aug;27(5):559-67
references.pmid: 20510771
references.citation: Antonie Van Leeuwenhoek. 1998 May;73(4):331-71
references.pmid: 9850420
references.citation: Int J Food Microbiol. 2014 Oct 1;188:83-91
references.pmid: 25087208
references.citation: Syst Biol. 2002 Jun;51(3):492-508
references.pmid: 12079646
references.citation: Appl Environ Microbiol. 1993 Jun;59(6):1838-41
references.pmid: 16348961
references.citation: Appl Environ Microbiol. 1987 Mar;53(3):495-9
references.pmid: 16347298
references.citation: Appl Environ Microbiol. 1995 Dec;61(12):4251-7
references.pmid: 8534092
references.citation: Int J Food Microbiol. 2014 Nov 17;191:1-9
references.pmid: 25218463
references.citation: Syst Appl Microbiol. 2002 Aug;25(2):287-93
references.pmid: 12353885
references.citation: Int J Food Microbiol. 1999 Feb 2;46(2):105-12
references.pmid: 10728611
references.citation: J Virol. 1994 Mar;68(3):1765-72
references.pmid: 8107238
references.citation: Lett Appl Microbiol. 2010 Jan;50(1):50-6
references.pmid: 19874485
references.citation: Int J Food Microbiol. 2005 Jun 25;102(1):73-83
references.pmid: 15925004
references.citation: Int J Food Microbiol. 2003 May 25;83(1):49-61
references.pmid: 12672592
references.citation: Int J Food Microbiol. 2010 Mar 31;138(1-2):85-90
references.pmid: 20116124
references.citation: Yeast. 1989 Apr;5 Spec No:S479-83
references.pmid: 2750315
references.citation: Food Microbiol. 2007 Feb;24(1):25-31
references.pmid: 16943091
references.citation: Appl Environ Microbiol. 2012 Oct;78(19):6838-49
references.pmid: 22820332
references.citation: Mycologia. 2007 Nov-Dec;99(6):842-58
references.pmid: 18333508
references.citation: J Appl Microbiol. 2001 Jul;91(1):182-90
references.pmid: 11442729
references.citation: Food Microbiol. 2015 Sep;50:70-7
references.pmid: 25998817
references.citation: Bioinformatics. 2010 Feb 1;26(3):419-20
references.pmid: 20080509
references.citation: Nat Commun. 2014 Jun 02;5:4044
references.pmid: 24887054
references.citation: Heredity (Edinb). 2001 Apr;86(Pt 4):469-79
references.pmid: 11520347
references.citation: FEMS Yeast Res. 2015 Aug;15(5):fov045
references.pmid: 26071435
references.citation: FEMS Yeast Res. 2006 Jan;6(1):102-11
references.pmid: 16423075
references.citation: Biotechnol Lett. 2007 Jul;29(7):1057-63
references.pmid: 17440687
references.citation: FEMS Microbiol Lett. 2015 Oct;362(20):null
references.pmid: 26391524
references.citation: World J Microbiol Biotechnol. 1991 Jul;7(4):485-9
references.pmid: 24425134
references.citation: Yeast. 2015 Aug;32(8):559-65
references.pmid: 26095929
references.citation: Yeast. 2004 May;21(7):549-56
references.pmid: 15164358
references.citation: Heredity (Edinb). 2004 Jun;92(6):508-18
references.pmid: 15014426
references.citation: J Med Microbiol. 1972 Feb;5(1):21-30
references.pmid: 4553908
references.citation: FEMS Yeast Res. 2010 Feb;10(1):58-71
references.pmid: 19840119
references.citation: Arch Microbiol. 1990;154(2):175-8
references.pmid: 2206104
references.citation: Mikrobiologiia. 2015 May-Jun;84(3):360-8
references.pmid: 26263696
references.citation: Springerplus. 2012 Dec;1(1):73
references.pmid: 23526575
references.citation: Lett Appl Microbiol. 1997 Jan;24(1):37-9
references.pmid: 9024002
references.citation: Int J Food Microbiol. 2014 May 16;178:87-97
references.pmid: 24681710
references.citation: FEMS Yeast Res. 2014 Jun;14(4):536-46
references.pmid: 24479773
references.citation: Int J Food Microbiol. 2008 Aug 15;126(1-2):195-201
references.pmid: 18597878
references.citation: Int J Syst Bacteriol. 1999 Jan;49 Pt 1:329-37
references.pmid: 10028278
references.citation: J Appl Bacteriol. 1990 May;68(5):491-4
references.pmid: 2196256
references.citation: Antonie Van Leeuwenhoek. 1995 Aug;68(2):151-60
references.pmid: 8546452
references.citation: J Agric Food Chem. 2007 Mar 7;55(5):2043-8
references.pmid: 17279767
references.citation: Int J Food Microbiol. 2005 Apr 1;99(3):237-43
references.pmid: 15808358
references.citation: Appl Environ Microbiol. 1995 Apr;61(4):1677
references.pmid: 16535013
references.citation: Antonie Van Leeuwenhoek. 1955;21(2):145-56
references.pmid: 14388635
references.citation: J Ind Microbiol Biotechnol. 2009 Feb;36(2):229-37
references.pmid: 18953586
references.citation: Int J Food Microbiol. 2015 Aug 3;206:67-74
references.pmid: 25956738
references.citation: J Appl Microbiol. 2003;95(3):457-62
references.pmid: 12911692
references.citation: Food Microbiol. 2013 May;34(1):207-14
references.pmid: 23498200
references.citation: FEMS Yeast Res. 2013 Feb;13(1):117-25
references.pmid: 23110448
references.citation: Bull Group Int Rech Sci Stomatol Odontol. 1994 Mar-Jun;37(1-2):13-7
references.pmid: 7994154
references.citation: Antonie Van Leeuwenhoek. 1995 Aug;68(2):91-9
references.pmid: 8546456
references.citation: J Appl Microbiol. 1999 Dec;87(6):949-956
references.pmid: 10692077
references.citation: Nat Biotechnol. 2000 Feb;18(2):233-4
references.pmid: 10657137
references.citation: Mol Biol Evol. 1999 Apr;16(4):525-43
references.pmid: 10331278
references.citation: Mol Ecol. 2004 Jul;13(7):2101-6
references.pmid: 15189230
references.citation: Front Microbiol. 2012 Apr 18;3:135
references.pmid: 22529841
references.citation: Int J Food Microbiol. 1991 Nov;14(2):153-60
references.pmid: 1777384
references.citation: BMC Microbiol. 2014 Sep 19;14:242
references.pmid: 25230758
references.citation: PLoS One. 2014 Apr 09;9(4):e94246
references.pmid: 24718638
references.citation: Lett Appl Microbiol. 2002;34(5):323-8
references.pmid: 11967053
references.citation: Int J Food Microbiol. 2008 Jun 10;124(3):231-8
references.pmid: 18457893
references.citation: J Wildl Dis. 1972 Oct;8(4):365-8
references.pmid: 4564184
references.citation: Int J Food Microbiol. 2006 Apr 25;108(2):239-45
references.pmid: 16487611
references.citation: Antonie Van Leeuwenhoek. 2001 Oct;80(1):85-92
references.pmid: 11761370
references.citation: J Ind Microbiol Biotechnol. 2008 Aug;35(8):851-7
references.pmid: 18425542
references.citation: Food Microbiol. 2014 Sep;42:188-95
references.pmid: 24929736
references.citation: Appl Environ Microbiol. 1985 Sep;50(3):727-8
references.pmid: 16346894
references.citation: J Appl Microbiol. 2005;98(1):136-44
references.pmid: 15610426
references.citation: Mycopathologia. 1998-1999;144(2):73-5
references.pmid: 10481287
references.citation: Bioinformatics. 2006 Jun 15;22(12):1540-2
references.pmid: 16595560