title: UCH-LI acts as a novel prognostic biomarker in gastric cardiac adenocarcinoma.
abstract: Gastric cardiac adenocarcinoma (GCA) accounts for a majority of gastric cancer population and harbors unfavorable outcome. Ubiquitin C-terminal hydrolase L1 (UCH-L1) belongs to the deubiquitinating enzyme family, which could regulate cell growth in human cancers. In the present study, expression of UCH-L1 was evaluated in 196 GCAs by immunohistochemistry using tissue microarray and its function on gastric cancer cells was measured. UCH-L1 expression was increased in GCA specimens, compared with their normal tissues and UCH-L1 overexpression is tightly correlated with tumor size and overall TNM stage. Log-rank analysis showed that UCH-L1 positive is reversely associated with cumulative survival (P<0.001). Multivariate Cox regression model showed that UCH-L1 overexpression is a remarkably negative predictor in GCA prognosis (Hazard Ratio=0.53, P<0.01), along with advanced TNM stage that is a known negative factor in gastric cancers (Hazard Ratio=0.33, P<0.05). Silencing of UCH-L1 reduced the ability of cell proliferation, colony formation, migration and invasion of gastric cancer cells. Our findings suggest that UCH-L1 is a promising prognostic biomarker for GCAs and might play an important role in the carcinogenesis of gastric cancer.
authors.affiliation: Laboratory for Interdisciplinary Research, Institution for Translational Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China; Department of Dermatovenerology, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Laboratory for Interdisciplinary Research, Institution for Translational Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China; Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Dermatovenerology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang Province, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Fang
authors.affiliation: Laboratory for Interdisciplinary Research, Institution for Translational Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China; Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China.
authors.forename: Rongying
authors.initials: R
authors.lastname: Ou
authors.affiliation: Laboratory for Interdisciplinary Research, Institution for Translational Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China; Department of Chemoradiotherapy, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory for Interdisciplinary Research, Institution for Translational Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China; Department of Dermatovenerology, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, Zhejiang Province, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D016009:Chi-Square Distribution; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D034622:RNA Interference; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D046888:Tissue Array Analysis; D014162:Transfection; D016896:Treatment Outcome; D047368:Tumor Burden; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase
keywords: Gastric cardiac adenocarcinoma; UCH-L1; biomarker; survival
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