title: Genome-scale estimate of the metabolic turnover of E. coli from the energy balance analysis.
abstract: In this article the notion of metabolic turnover is revisited in the light of recent results of out-of-equilibrium thermodynamics. By means of Monte Carlo methods we perform an exact sampling of the enzymatic fluxes in a genome scale metabolic network of E. coli in stationary growth conditions from which we infer the metabolites turnover times. However the latter are inferred from net fluxes, and we argue that this approximation is not valid for enzymes working nearby thermodynamic equilibrium. We recalculate turnover times from total fluxes by performing an energy balance analysis of the network and recurring to the fluctuation theorem. We find in many cases values one of order of magnitude lower, implying a faster picture of intermediate metabolism.
authors.affiliation: Institute of Science and Technology Austria (IST Austria), Am Campus 1, Klosterneuburg A-3400, Austria. Center for life nanoscience, Istituto Italiano di Tecnologia, CLNS-IIT, Viale Regina Elena 291, I-00161, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: De Martino
mesh_terms: D004734:Energy Metabolism; D004926:Escherichia coli / Q000378:metabolism*; D016680:Genome, Bacterial; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009010:Monte Carlo Method; D013816:Thermodynamics