title: Oxidative changes in the blood and serum albumin differentiate rats with monoarthritis and polyarthritis.
abstract: Adjuvant arthritis in rats, as rheumatoid arthritis in humans, may be of greater or lesser severity, namely polyarthritis and monoarthritis, respectively. The present study was planned to evaluate the oxidative changes in the blood and specifically in the serum albumin of rats with adjuvant-induced mono- and poly-arthritis. Total antioxidant capacity, thiols, carbonyl groups, albumin, uric acid and ascorbic acid were measured in the total serum. The specific oxidative status of albumin was also measured after separation by affinity chromatography. All serum oxidative parameters were close to normal in monoarthritic rats with the exception of the ascorbic acid concentration, which was 23 % lower, and albumin carbonyl groups, which were 64 % higher. Many modifications were found in polyarthritic rats, specially the ascorbic acid concentration (35 % lower) and albumin carbonyl groups (102 % higher). The results revealed that the levels of ascorbic acid in the serum and carbonyl groups in the albumin molecule can be regarded as indicators of the severity of arthritis since they were modified by both monoarthritis and polyarthritis, but to different degrees.
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Adelar
authors.initials: A
authors.lastname: Bracht
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Sandra Silva
authors.initials: SS
authors.lastname: Silveira
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Cristiane Vizioli
authors.initials: CV
authors.lastname: Castro-Ghizoni
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Anacharis Babeto
authors.initials: AB
authors.lastname: Sá-Nakanishi
authors.affiliation: Department of Clinical Analysis, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Márcia Rosângela Neves
authors.initials: MR
authors.lastname: Oliveira
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Ciomar Aparecida
authors.initials: CA
authors.lastname: Bersani-Amado
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Rosane Marina
authors.initials: RM
authors.lastname: Peralta
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá, 87020900 Brazil.
authors.forename: Jurandir Fernando
authors.initials: JF
authors.lastname: Comar
keywords: Adjuvant-induced arthritis; Antioxidant status; Chronic inflammation; Serum albumin oxidation
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