title: Absence of human rhinovirus and respiratory syncytial virus from bronchoalveolar lavage and bronchial biopsies of selected patients with stable chronic obstructive pulmonary disease.
abstract: Previous studies have reported very different rates of human rhinovirus (HRV) and respiratory syncytial virus (RSV) genome detection in nasal and sputum samples, but not in bronchoalveolar lavage (BAL) and bronchial biopsy samples. Our study aimed to investigate the presence of HRV and RSV in the lungs of 31 consecutive patients with stable COPD (11 GOLD stage I, 11 II, and 9 III) and 22 control subjects (12 current or past smokers, and 10 non-smokers), who underwent diagnostic (e.g., lung cancer) and/or therapeutic (e.g., hemoptysis) fibreoptic bronchoscopy in a university hospital in Athens, Greece. Viral RNA of HRV and RSV were not detected in any of the samples of COPD patients or control subjects after being processed with real-time PCR.
authors.affiliation: 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. stelagiannakaki@hotmail.com.
authors.forename: Styliani
authors.initials: S
authors.lastname: Giannakaki
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece. lpoliti@med.uoa.gr.
authors.forename: Lida
authors.initials: L
authors.lastname: Politi
authors.affiliation: 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. kantonogiannaki@gmail.com.
authors.forename: Elvira Markella
authors.initials: EM
authors.lastname: Antonogiannaki
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece. nspan@med.uoa.gr.
authors.forename: Nick
authors.initials: N
authors.lastname: Spanakis
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece. georgearsenis@yahoo.com.
authors.forename: Georgios
authors.initials: G
authors.lastname: Arsenis
authors.affiliation: 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. filaditaki@yahoo.com.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Filaditaki
authors.affiliation: 1st Department of Critical Care and Pulmonary Services, Evangelismos Hospital, Medical School, University of Athens, Athens, Greece. szakynthinos@yahoo.com.
authors.forename: Spyridon
authors.initials: S
authors.lastname: Zakynthinos
authors.affiliation: 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. nap@karagianidis.gr.
authors.forename: Napoleon
authors.initials: N
authors.lastname: Karagiannidis
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece. atsakris@med.uoa.gr.
authors.forename: Athanassios
authors.initials: A
authors.lastname: Tsakris
mesh_terms: D000368:Aged; D001980:Bronchi / Q000473:pathology / Q000821:virology*; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012229:Rhinovirus / Q000302:isolation & purification*; D012720:Severity of Illness Index; D013183:Sputum / Q000821:virology
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