title: Magnetically Responsive Bone Marrow Mesenchymal Stem Cell-Derived Smooth Muscle Cells Maintain Their Benefits to Augmenting Elastic Matrix Neoassembly.
abstract: Abdominal aortic aneurysms (AAA) represent abnormal aortal expansions that result from chronic proteolytic breakdown of elastin and collagen fibers by matrix metalloproteases. Poor elastogenesis by adult vascular smooth muscle cells (SMCs) limits regenerative repair of elastic fibers, critical for AAA growth arrest. Toward overcoming these limitations, we recently demonstrated significant elastogenesis by bone marrow mesenchymal stem cell-derived SMCs (BM-SMCs) and their proelastogenesis and antiproteolytic effects on rat aneurysmal SMCs (EaRASMCs). We currently investigate the effects of super paramagnetic iron oxide nanoparticle (SPION) labeling of BM-SMCs, necessary to magnetically guide them to the AAA wall, on their functional benefits. Our results indicate that SPION-labeling is noncytotoxic and does not adversely impact the phenotype and elastogenesis by BM-SMCs. In addition, SPION-BM-SMCs showed no changes in the ability of the BM-SMCs to stimulate elastin regeneration and attenuate proteolytic activity by EaRASMCs. Together, our results are promising toward the utility of SPIONs for magnetic targeting of BM-SMCs for in situ AAA regenerative repair.
authors.affiliation: 1 Department of Biomedical Engineering, Cleveland Clinic , Cleveland, Ohio.|2 Department of Biology, University of Akron , Akron, Ohio.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Swaminathan
authors.affiliation: 1 Department of Biomedical Engineering, Cleveland Clinic , Cleveland, Ohio.
authors.forename: Balakrishnan
authors.initials: B
authors.lastname: Sivaraman
authors.affiliation: 1 Department of Biomedical Engineering, Cleveland Clinic , Cleveland, Ohio.
authors.forename: Lee
authors.initials: L
authors.lastname: Moore
authors.affiliation: 1 Department of Biomedical Engineering, Cleveland Clinic , Cleveland, Ohio.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zborowski
authors.affiliation: 1 Department of Biomedical Engineering, Cleveland Clinic , Cleveland, Ohio.
authors.forename: Anand
authors.initials: A
authors.lastname: Ramamurthi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D004547:Elastic Tissue / Q000378:metabolism*; D004548:Elasticity; D005109:Extracellular Matrix / Q000378:metabolism; D060526:Magnetic Fields; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
references.citation: J Neurosci. 2013 Nov 27;33(48):18880-92
references.pmid: 24285894
references.citation: FASEB J. 2008 Dec;22(12):4239-47
references.pmid: 18725459
references.citation: J Surg Res. 1993 Jun;54(6):571-4
references.pmid: 8412067
references.citation: Biomaterials. 2013 Mar;34(8):1987-94
references.pmid: 23237516
references.citation: Nat Mater. 2014 Feb;13(2):125-38
references.pmid: 24452356
references.citation: J Cell Biochem. 2003 Mar 1;88(4):660-72
references.pmid: 12577300
references.citation: Bioconjug Chem. 2008 Mar;19(3):740-50
references.pmid: 18288791
references.citation: Tissue Eng Part A. 2009 Mar;15(3):501-11
references.pmid: 18847364
references.citation: Tissue Eng Part A. 2011 Jul;17(13-14):1699-711
references.pmid: 21341992
references.citation: Tissue Eng Part A. 2011 Aug;17(15-16):1945-58
references.pmid: 21417692
references.citation: J Tissue Eng Regen Med. 2012 Oct;6(9):673-86
references.pmid: 21953981
references.citation: J Mol Med (Berl). 2005 May;83(5):377-85
references.pmid: 15688234
references.citation: Int J Nanomedicine. 2014;9:273-87
references.pmid: 24421639
references.citation: J Biol Chem. 1989 Nov 5;264(31):18272-5
references.pmid: 2681193
references.citation: Acta Biomater. 2013 May;9(5):6511-25
references.pmid: 23376127
references.citation: Circ Res. 1995 Dec;77(6):1107-13
references.pmid: 7586222
references.citation: Clin Sci (Lond). 1991 Aug;81(2):233-9
references.pmid: 1653668
references.citation: Circulation. 1990 Sep;82(3):973-81
references.pmid: 2144219
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):10211-6
references.pmid: 17553967
references.citation: Magn Reson Med. 2006 Jan;55(1):203-9
references.pmid: 16315206
references.citation: Circ Cardiovasc Imaging. 2012 Sep 1;5(5):559-65
references.pmid: 22875883
references.citation: Br J Surg. 1999 Aug;86(8):1053-8
references.pmid: 10460642
references.citation: J Vasc Surg. 1996 Feb;23(2):336-46
references.pmid: 8637112
references.citation: Differentiation. 1991 Jul;47(2):99-105
references.pmid: 1955111
references.citation: Acta Biomater. 2014 Jan;10(1):163-72
references.pmid: 24016842
references.citation: Methods Mol Biol. 2012;906:239-52
references.pmid: 22791437
references.citation: Biotechnol Bioeng. 2001 Dec 20;75(6):642-55
references.pmid: 11745142
references.citation: J Nanobiotechnology. 2011;9:4
references.pmid: 21542946
references.citation: J Cardiovasc Magn Reson. 2014;16:7
references.pmid: 24406054
references.citation: BMC Biotechnol. 2008;8:47
references.pmid: 18466619
references.citation: J Orthop Res. 2012 Nov;30(11):1725-31
references.pmid: 22618690
references.citation: Ann Surg. 1979 Feb;189(2):158-64
references.pmid: 154875
references.citation: J Tissue Eng Regen Med. 2014 Nov 6;:null
references.pmid: 25376929
references.citation: Circ Res. 1992 Sep;71(3):711-9
references.pmid: 1339321
references.citation: Acc Chem Res. 2011 Oct 18;44(10):853-62
references.pmid: 21528865
references.citation: Acta Biomater. 2013 Dec;9(12):9292-302
references.pmid: 23917150
references.citation: Biomaterials. 2007 Jun;28(16):2572-81
references.pmid: 17320946
references.citation: J Mater Sci Mater Med. 2012 Jul;23(7):1685-95
references.pmid: 22481628
references.citation: Cardiovasc Res. 2002 Sep;55(4):850-63
references.pmid: 12176134
references.citation: Physiol Rev. 2004 Jul;84(3):767-801
references.pmid: 15269336
references.citation: Anal Biochem. 1980 Mar 1;102(2):344-52
references.pmid: 6158890
references.citation: Physiol Rev. 2009 Jul;89(3):957-89
references.pmid: 19584318
references.citation: In Vitro Cell Dev Biol. 1985 Dec;21(12):674-80
references.pmid: 3908442