title: Determination of some psychotropic drugs in serum and saliva samples by HPLC-DAD and HPLC MS.
abstract: A simple, rapid and sensitive HPLC-DAD method has been developed and validated for the simultaneous determination of seven psychotropic drugs (risperidone, citalopram, clozapine,quetiapine, levomepromazine, perazine and aripiprazole) in human serum or saliva samples. The chromatographic analyses were performed on a XSELECT CSH Phenyl-Hexyl column with a mobile phase containing methanol, acetate buffer at pH 3.5 and 0.025mL(-1) diethylamine. The influence of concentration of methanol in injection samples and injection volume on peak symmetry and system efficiency was examined.The full separation of all investigated drugs, good peaks' symmetry and simultaneously high systems efficiency were obtained in applied chromatographic system. The method is suitable for the analysis of investigated drugs in human plasma or saliva for psychiatric patients for control of pharmacotherapy, particularly in combination therapy. HPLC-MS was applied for verification of the presence of drugs and their metabolites in serum and saliva samples from patients.
authors.affiliation: Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a 20-093 Lublin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Petruczynik
authors.affiliation: Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a 20-093 Lublin, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Wróblewski
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Faculty of Chemistry Gagarina 7, PL-87-100 Torun, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Szultka-Młyńska
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Faculty of Chemistry Gagarina 7, PL-87-100 Torun, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Buszewski
authors.affiliation: Department of Clinical Neuropsychiatry, Medical University of Lublin, Głuska 2, 20-439 Lublin, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Karakuła-Juchnowicz
authors.affiliation: Department of Clinical Neuropsychiatry, Medical University of Lublin, Głuska 2, 20-439 Lublin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Gajewski
authors.affiliation: Department of Clinical Neuropsychiatry, Medical University of Lublin, Głuska 2, 20-439 Lublin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Morylowska-Topolska
authors.affiliation: Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a 20-093 Lublin, Poland. Electronic address: monika.hajnos@umlub.pl.
authors.forename: M
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D011619:Psychotropic Drugs / Q000032:analysis / Q000097:blood* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*
chemical_list: D011619:Psychotropic Drugs
keywords: HPLC–DAD; HPLC–MS; Human saliva; Human serum; Psychotropic drugs