title: Genetic analysis of TNFST15 variants in ankylosing spondylitis.
abstract: OBJECTIVE
  The purpose of this study was to explore the role of TNF-like ligand 1A (TL1A) gene (TNFST15) polymorphisms (rs3810936, rs7848647, and rs6478109) in the generation of ankylosing spondylitis (AS).
  
  
  METHODS
  Polymerase chain reaction (PCR) and sequencing were used to conduct the genotyping of TNFSF15 polymorphisms in 113 AS patients and 120 healthy persons as the case and control groups. The frequencies comparison was performed by chi-square or t test between the two groups. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated to represent the correlation between TNFSF15 polymorphism and AS. Besides, genotypes distribution of the former in controls was checked by Hardy-Weinberg equilibrium (HWE).
  
  
  RESULTS
  There was statistically significant difference in AS patients and controls based on family history. Among TNFSF15 polymorphisms, only TT genotype frequency of rs3810936 in cases was obviously high, compared with the controls (P=0.04), the results indicated that TT was a high-risk genotype (OR=2.31, 95% CI=1.03-5.20). However, both of rs6478109, rs7848647 polymorphisms didn't show any association with AS.
  
  
  CONCLUSIONS
  Rs3810936 of TNFSF15 were related to the risk of AS and we should pay more attention to the role of TNFSF15 polymorphisms in the pathogenesis of AS in the future.
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Nai-Guo
authors.initials: NG
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Tan
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Ze-Nong
authors.initials: ZN
authors.lastname: Yuan
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Wang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D013167:Spondylitis, Ankylosing / Q000235:genetics*; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15 / Q000235:genetics*
chemical_list: C117026:TNFSF15 protein, human; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15
keywords: TNFSF15; ankylosing spondylitis; polymorphism
references.citation: Arthritis Rheum. 1983 Feb;26(2):186-90
references.pmid: 6600615
references.citation: Natl Med J India. 2013 Nov-Dec;26(6):338-9
references.pmid: 25073991
references.citation: Int J Clin Exp Med. 2015 May 15;8(5):7679-83
references.pmid: 26221317
references.citation: PLoS One. 2014;9(9):e108321
references.pmid: 25251497
references.citation: Cytokine. 2013 Oct;64(1):234-42
references.pmid: 23859810
references.citation: Immunity. 2002 Mar;16(3):479-92
references.pmid: 11911831
references.citation: Ann Rheum Dis. 2004 Dec;63(12):1601-4
references.pmid: 15297280
references.citation: Curr Allergy Asthma Rep. 2015 Jan;15(1):489
references.pmid: 25447326
references.citation: J Immunol. 1993 May 1;150(9):4168-78
references.pmid: 8473755
references.citation: Cytokine. 2014 Sep;69(1):125-30
references.pmid: 24832108
references.citation: Rev Med Chil. 2014 Sep;142(9):1165-73
references.pmid: 25517057
references.citation: Turk J Med Sci. 2014;44(4):595-600
references.pmid: 25551928
references.citation: Bull NYU Hosp Jt Dis. 2010;68(3):171-4
references.pmid: 20969547
references.citation: Br J Rheumatol. 1997 Jul;36(7):766-71
references.pmid: 9255111
references.citation: Semin Arthritis Rheum. 2007 Aug;37(1):39-47
references.pmid: 17350674
references.citation: Nat Genet. 2004 Jul;36(7):664
references.pmid: 15226743
references.citation: Scand J Rheumatol. 1998;27(3):219-24
references.pmid: 9645418
references.citation: Rheumatol Int. 2015 Jun;35(6):945-51
references.pmid: 25385438
references.citation: Drug Dev Res. 2014 Nov;75 Suppl 1:S15-9
references.pmid: 25381967
references.citation: Physiotherapy. 2016 Jun;102(2):202-9
references.pmid: 26119877
references.citation: Biosci Trends. 2013 Apr;7(2):89-92
references.pmid: 23612078
references.citation: Arthritis Rheum. 2013 Jun;65(6):1522-9
references.pmid: 23508523
references.citation: Mediators Inflamm. 2013;2013:258164
references.pmid: 24453414
references.citation: J Rheumatol. 1988 Jul;15(7):1109-14
references.pmid: 3262757
references.citation: Tumour Biol. 2010 Oct;31(5):443-9
references.pmid: 20567955
references.citation: PLoS One. 2015;10(6):e0130982
references.pmid: 26103568
references.citation: PLoS One. 2013;8(9):e74794
references.pmid: 24069348
references.citation: Rheumatology (Oxford). 2013 Mar;52(3):448-51
references.pmid: 23204549
references.citation: Z Rheumatol. 2010 Jul;69(5):397-402
references.pmid: 20544353
references.citation: Mucosal Immunol. 2014 Nov;7(6):1492-503
references.pmid: 24850426
references.citation: Genet Test Mol Biomarkers. 2015 Jan;19(1):37-43
references.pmid: 25494292