title: Roles of Cx43 and AKAP95 in ovarian cancer tissues in G1/S phase.
abstract: OBJECTIVE
  The purpose of this study was to investigate the expression of A-kinase anchor protein 95 (AKAP95), cell cycle protein E1 (cyclinE1) and D1 (cyclinD1), and gap junction protein connexin 43 (Cx43) in ovarian cancer tissues, the relationship between four proteins and clinicopathologic parameters, and the correlation between these proteins.
  
  
  METHODS
  The expression of proteins in 54 cases of ovarian cancer tissues was detected by immunohistochemical method.
  
  
  RESULTS
  The positive expression rates of AKAP95, cyclinD1 and cyclinE1 in ovarian cancer tissues were 72.22%, 66.67% and 79.63%, respectively, which were higher than that of ovarian pericarcinoma tissues expressing as 33.33%, 25% and 8.30% (P<0.05). The positive expression rate of Cx43 in ovarian cancer tissues was 40.74%, which was lower than that of ovarian pericarcinoma tissues expressing as 75%; respectively, and the difference was statistically significant between groups (P<0.05). The expression of cyclinD1 in ovarian cancer tissues was related to the histologic type (P<0.05) while it showed no correlation with the degree of differentiation (P>0.05). Additionally, the expression of AKAP95, Cx43 and cyclinE1 in ovarian cancer tissues showed no correlation with the degree of differentiation or the histologic type (P>0.05). Protein expressions of AKAP95, Cx43 and cyclinE1 were correlated with each other (P<0.05), and the expressions of cyclinD1, cyclinE1 and Cx43 were also correlated with each other (P<0.05). However, AKAP95 and cyclinD1 showed no correlation (P>0.05).
  
  
  CONCLUSIONS
  AKAP95, cyclinD1 and cyclinE1 play an important role in promoting the process of ovarian cancer formation. The tumor inhibitory effects of Cx43 protein on the pathogenesis of ovarian cancer were weakened. The expression of cyclinD1 in ovarian cancer tissues is related to the histologic type while it shows no correlation with the degree of differentiation. Additionally, the expression of AKAP95, Cx43 and cyclinE1 in ovarian cancer tissues shows no correlation with the degree of differentiation or the histologic type. AKAP95 expression is correlated with Cx43 and cyclinE1 expression; Cx43 expression is correlated with AKAP95, cyclinD1 and cyclinE1 expression; cyclinE1 expression is correlated with AKAP95, Cx43, cyclinD1 expression; cyclinD1 expression is correlated with Cx43 and cyclinE1 expression, while AKAP95 and cyclinD1 show no correlation.
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University Dalian 116001, PR China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University Dalian 116001, PR China.
authors.forename: Suhang
authors.initials: S
authors.lastname: Hua
authors.affiliation: School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University Shenyang 110016, Liaoning, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Bogang
authors.initials: B
authors.lastname: Teng
authors.affiliation: The First Affiliated Hospital of Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Xiuyi
authors.initials: X
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University Xiamen 361102, Fujian, PR China.
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D054758:A Kinase Anchor Proteins / Q000032:analysis*; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D002454:Cell Differentiation; D018031:Connexin 43 / Q000032:analysis*; D019938:Cyclin D1 / Q000032:analysis; D019927:Cyclin E / Q000032:analysis; D005260:Female; D016193:G1 Phase; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D015513:Oncogene Proteins / Q000032:analysis; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology; D016196:S Phase
chemical_list: D054758:A Kinase Anchor Proteins; C485099:AKAP8 protein, human; D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; C475820:CCNE1 protein, human; D018031:Connexin 43; D019927:Cyclin E; C500216:GJA1 protein, human; D015513:Oncogene Proteins; D019938:Cyclin D1
keywords: AKAP95; Cx43; correlation; cyclinD1; cyclinE1; ovarian cancer
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