title: The carbonic anhydrase domain of plant mitochondrial complex I.
abstract: The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling gamma-type carbonic anhydrases. The Arabidopsis genome codes for five complex I-integrated gamma-type carbonic anhydrases (γCA1, γCA2, γCA3, γCAL1, γCAL2), but only three copies of this group of proteins form an individual extra domain. Biochemical analyses revealed that the domain is composed of one copy of either γCAL1 or γCAL2 plus two copies of the γCA1/γCA2 proteins. Thus, the carbonic anhydrase domain can have six distinct subunit configurations. Single and double mutants with respect to the γCA/γCAL proteins were employed to genetically dissect the function of the domain. New insights into complex I biology in plants will be reviewed and discussed.
authors.affiliation: Institut für Pflanzengenetik, Leibniz Universität Hannover, Hannover, 30419, Germany.|Institut für Botanik, Leibniz Universität Hannover, Hannover, 30419, Germany.
authors.forename: Steffanie
authors.initials: S
authors.lastname: Fromm
authors.affiliation: Institut für Pflanzengenetik, Leibniz Universität Hannover, Hannover, 30419, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Senkler
authors.affiliation: Instituto de Investigaciones Biológicas IIB, CONICET, National University of Mar del Plata, Mar del Plata, 7600, Argentina.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Zabaleta
authors.affiliation: Institut für Botanik, Leibniz Universität Hannover, Hannover, 30419, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Peterhänsel
authors.affiliation: Institut für Pflanzengenetik, Leibniz Universität Hannover, Hannover, 30419, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Braun
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D042967:Electron Transport Complex I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation
chemical_list: D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; D002256:Carbonic Anhydrases; D042967:Electron Transport Complex I