title: Effect of A One-Week Balanced Diet on Expression of Genes Related to Zinc Metabolism and Inflammation in Type 2 Diabetic Patients.
abstract: To evaluate the effect of diet on metabolic control and zinc metabolism in patients with type 2 diabetes mellitus (T2DM). One-week balanced diet was provided to 10 Brazilians patients with T2DM. Nutritional assessment, laboratorial parameters and expression of zinc transporter and inflammatory genes in peripheral blood mononuclear cells (PBMC) were performed. Healthy non-diabetic subjects of the same demographic were recruited to provide baseline data. Diabetic patients had higher body mass index and greater fasting plasma glucose, plasma tumor necrosis factor α (TNFα) and plasma interleukin 6 (IL6) levels compared with healthy subjects. In addition, the expression of transporters 4 (ZnT4) mRNA was lower and IL6 mRNA was higher in PBMC of these diabetic patients than in healthy subject. One week after a balanced diet was provided, fasting plasma glucose decreased significantly as did TNFα, IL6 and Metallothionein 1 (MT1) mRNAs. No change was observed in zinc transporter expression in PBMC after the dietary intervention. A healthy eating pattern maintained for one week was able to improve metabolic control of diabetic patients by lowering fasting plasma glucose. This metabolic control may be related to down-regulation of zinc-related transcripts from PBMCs, as TNFα, IL6 and MT1 mRNA.
authors.affiliation: Department of Nutrition, Federal University of Rio Grande do Norte, Natal 59084-100, Brazil.
authors.forename: Lucia Leite
authors.identifier: 0000-0002-8061-7048
authors.initials: LL
authors.lastname: Lais
authors.affiliation: Department of Nutrition, Federal University of Rio Grande do Norte, Natal 59084-100, Brazil.
authors.forename: Sancha Helena
authors.initials: SH
authors.lastname: de Lima Vale
authors.affiliation: Department of Nutrition, Potiguar University, Natal 59056-000, Brazil.
authors.forename: Camila Alves
authors.initials: CA
authors.lastname: Xavier
authors.affiliation: Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa 58051-900, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: de Araujo Silva
authors.affiliation: Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, United States.
authors.forename: Tolunay Beker
authors.initials: TB
authors.lastname: Aydemir
authors.affiliation: Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, United States.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Cousins
keywords: Diet; Nutrigenomics; Type 2 diabetes mellitus; Zinc
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