title: F-127-PEI co-delivering docetaxel and TFPI-2 plasmid for nasopharyngeal cancer therapy.
abstract: The co-delivery of drug and gene has become the primary strategy in cancer therapy. However, to construct one safe co-delivering system with higher drug loading and gene transfection efficiency for cancer therapy is still challenging. Herein, a novel degradable nanocarriers were synthesized and characterized in this study, which was composed of polyethylenimine (PEI)-linked PEO-PPO-PEO (Pluronic F127), called F127-PEI. Then the nanocarrier was used for hydrophobic docetaxel (DOC) and functional gene (TFPI-2 plasmid) co-delivery to treat nasopharyngeal cancer (NPC). The results indicated that F127-PEI nanocarriers had higher DOC loading amount and possessed good gene delivery effect in vitro. For co-delivery analysis, the obtained F127-PEI/DOC/TFPI-2 complexes could induce a more significant apoptosis than DOC or TFPI-2 alone, and decreased invasive capacity of NPC HNE-1 cells more obviously. Moreover, the F127-PEI copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k by the hemolysis and MTT assays, which suggests a promising potential for NPC therapy.
authors.affiliation: Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Electronic address: taoliu18@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangxi Key Laboratory of Hematological Oncology and Cell Biology, Jiangxi Provincial People's Hospital, Nanchang 330006, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ke
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Jiangxi Institute of Materia Medica,Nanchang 330029, China.
authors.forename: Xidong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Light Chemical Engineering, Guangdong Polytechnic, Foshan 528041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, Shenzhen 518036, China. Electronic address: nghui@21cn.com.
authors.forename: Guohui
authors.initials: G
authors.lastname: Nie
mesh_terms: D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D010957:Plasmids / Q000737:chemistry / Q000494:pharmacology; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011409:Propylene Glycols / Q000737:chemistry / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; C116176:PEO-PPO-PEO; D011409:Propylene Glycols; C021240:tissue-factor-pathway inhibitor 2; D020442:Poloxamer; D011092:Polyethylene Glycols
keywords: Co-delivery; Docetaxel; Nasopharyngeal cancer; TFPI-2