title: Design and Characterization of a Novel p1025 Peptide-Loaded Liquid Crystalline System for the Treatment of Dental Caries.
abstract: Dental caries, mainly caused by the adhesion of Streptococcus mutans to pellicle-coated tooth surfaces, is an important public health problem worldwide. A synthetic peptide (p1025) corresponding to residues 1025-1044 of the adhesin can inhibit this binding. Peptides are particularly susceptible to the biological environment; therefore, a p1025 peptide-loaded liquid crystalline system (LCS) consisting of tea tree oil as the oil phase, polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl alcohol as the surfactant, and water or 0.5% polycarbophil polymer dispersions as the aqueous phase was employed as a drug delivery platform. This system exhibited anticaries and bioadhesive properties and provided a protective environment to p1025 at the site of action, thereby modulating its action, prolonging its contact with the teeth, and decreasing the frequency of administration. LCSs were characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), and rheological, texture, and bioadhesive tests. PLM and SAXS revealed the presence of hexagonal liquid crystalline phases and microemulsions. Rheological analyses demonstrated that the addition of polymer dispersions favored characteristics such as shear thinning and thixotropy, hence improving buccal application. Bioadhesion tests showed that polymer dispersions contributed to the adhesion onto the teeth. Taken together, LCS could provide a novel pharmaceutical nanotechnology platform for dental caries treatment.
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Campus Araraquara, Araraquara, SP 14800-850, Brazil. calixtogmf@fcfar.unesp.br.
authors.forename: Giovana Maria Fioramonti
authors.initials: GM
authors.lastname: Calixto
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Campus Araraquara, Araraquara, SP 14800-850, Brazil. matheusgarcia3@hotmail.com.
authors.forename: Matheus Henrique
authors.initials: MH
authors.lastname: Garcia
authors.affiliation: Instituto de Química, UNESP-Universidade Estadual Paulista, Campus Araraquara, Araraquara, SP 14800-900, Brazil. eduardocilli@gmail.com.
authors.forename: Eduardo Maffud
authors.initials: EM
authors.lastname: Cilli
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Campus Araraquara, Araraquara, SP 14800-850, Brazil. leila@fcfar.unesp.br.
authors.forename: Leila Aparecida
authors.initials: LA
authors.lastname: Chiavacci
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Campus Araraquara, Araraquara, SP 14800-850, Brazil. chorilli@fcfar.unesp.br.
authors.forename: Marlus
authors.initials: M
authors.lastname: Chorilli
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D003731:Dental Caries / Q000188:drug therapy* / Q000382:microbiology; D016503:Drug Delivery Systems; D015195:Drug Design; D006801:Humans; D050866:Liquid Crystals / Q000737:chemistry*; D036103:Nanotechnology; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D012212:Rheology; D012463:Saliva / Q000187:drug effects*; D053838:Scattering, Small Angle; D013295:Streptococcus mutans / Q000187:drug effects; D013501:Surface-Active Agents / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D018829:Adhesins, Bacterial; D010455:Peptides; D013501:Surface-Active Agents
keywords: bioadhesive polymers; dental caries; liquid crystalline system; nanostructured drug delivery systems; tea tree oil
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