title: SATB1 expression is correlated with β-catenin associated epithelial-mesenchymal transition in colorectal cancer.
abstract: SATB1, a global gene regulator, has been implicated in the growth and metastasis of multiple cancers, including colorectal cancer. While the understanding about the role of SATB1 in CRC remains limited. The aim of our study is to investigate the expression of SATB1 in CRC, and the relationship between SATB1 expression pattern and clinicopathological variables. A further aim is to analyze the correlation between SATB1 expression and epithelial-mesenchymal transition in CRC. Immunohistochemical expression of SATB1, β-catenin, E-cadherin, CK20, Vimentin, SMA, and desmin were assessed in a cohort of 200 patients using tissue microarrays. SATB1 was expressed in 133 (66.5%) CRC primary lesions, 14 (28%) adjacent colorectal mucosa specimens, and 60 (75%) corresponding lymph node metastases. The expression level of SATB1 was significantly higher in lymph node metastases than in CRC primary lesions and normal mucosa (P = 0.000). High expression of SATB1 in CRC was strongly correlated with poor differentiation of tumor tissues (P = 0.000). High expression of SATB1 was significantly correlated with aberrant expression of β-catenin (P = 0.0005), low expression of E-cadherin (P = 0.000) and CK20 (P = 0.000) and with high expression of Vimentin (P = 0.001). No SMA or desmin protein was expressed in the CRC cells. Our results suggested that high expression of SATB1 is significantly correlated with poor differentiation of CRC. SATB1 might promote the epithelial-mesenchymal transition by increasing the aberrant expression of β-catenin.
authors.affiliation: a Department of Pathology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.|b Department of Pathology , the Suzhou Hospital Affiliated to Nanjing Medical University , Suzhou , China.
authors.forename: Jing-huan
authors.initials: JH
authors.lastname: Lv
authors.affiliation: b Department of Pathology , the Suzhou Hospital Affiliated to Nanjing Medical University , Suzhou , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: b Department of Pathology , the Suzhou Hospital Affiliated to Nanjing Medical University , Suzhou , China.
authors.forename: Ming-hong
authors.initials: MH
authors.lastname: Shen
authors.affiliation: a Department of Pathology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Department of Pathology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human; D051176:beta Catenin
keywords: Colorectal cancer; SATB1; epithelial-mesenchymal transition; immunochemistry; β-catenin
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