title: In vivo analyses of the effects of polyamidoamine dendrimer on dentin biomineralization and dentinal tubules occlusion.
abstract: This study evaluated the biomineralization and dentinal tubules occlusion abilities of the carboxyl-terminated polyamidoamine dendrimer (PAMAM-COOH) on human demineralized dentin in vivo at different time points. Demineralization dentin model with and without treated with PAMAM-COOH were sutured to the interior side of the rat's cheeks, that was incubated in the rats' saliva for 2, 4 and 6 weeks respectively. Finally, the newly formed precipitates were characterized by SEM, EDS, XRD and microhardness test. The hydroxyapatite (HA) on the dentin treated with PAMAM-COOH were formed gradually with the time going by, and the regenerated HA has a similar crystal structure with natural dentin, whereas the crystallites did not exist on the control group. The microhardness of PAMAM-COOH-applied specimens had a significantly higher than those without application. These results suggest that the PAMAM-COOH promoted the biomineralization of demineralized dentin and displayed favourable effects on blocking the open dentinal tubules.
authors.affiliation: Department of Endodontics, Stomatological Hospital, Guangxi Medical University.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Xie
authors.forename: Xiaolang
authors.initials: X
authors.lastname: Wei
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Zhou
mesh_terms: D000818:Animals; D050091:Dendrimers / Q000494:pharmacology*; D003804:Dentin / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000517:prevention & control*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011073:Polyamines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012463:Saliva / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D017001:Tooth Demineralization / Q000188:drug therapy*; D014961:X-Ray Diffraction
chemical_list: D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines