title: Antisense expression of Gossypium barbadense UGD6 in Arabidopsis thaliana significantly alters cell wall composition.
abstract: Uridine diphosphate-glucose dehydrogenase (UGD, EC1.1.1.22 oxidizes UDP-Glc (UDP-D-glucose) to UDP-GlcA (UDP-D-glucuronate), a critical precursor of cell wall polysaccharides. GbUGD6 from Gossypium barbadense is more highly expressed late in the elongation of cotton fibers (15 d post-anthesis (DPA)) and during the stage of secondary cell wall thickening (30 DPA). Subcellular localization analysis in onion epidermis revealed that fluorescently labeled GbUGD6 protein was distributed throughout the cell membrane, as well as the nucleus and vacuoles. Examination of UGD function in Arabidopsis revealed that the antisense GbUGD6 lines had shorter roots, deferred blossoming, compared to wild-type plants. Activities of associated enzymes were also affected by UGD reduction, and biochemical analysis of cell wall samples showed an increase in cellulose levels and a decrease in UGP-GlcA contents. The results of the present study as well as previous studies on UGD support the conclusion that UGD plays a major role in synthesizing polysaccharides synthesis in the cell wall.
authors.affiliation: North China Key Laboratory for Germplasm Resources of Education Ministry; Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: North China Key Laboratory for Germplasm Resources of Education Ministry; Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, 071001, China.|North China University Science and Technology, Tangshan, 063009, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Pan
authors.affiliation: North China Key Laboratory for Germplasm Resources of Education Ministry; Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Xingfen
authors.initials: X
authors.lastname: Wang
authors.affiliation: North China Key Laboratory for Germplasm Resources of Education Ministry; Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: North China Key Laboratory for Germplasm Resources of Education Ministry; Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, 071001, China. mzhy@hebau.edu.cn.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Ma
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002473:Cell Wall / Q000378:metabolism*; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D049452:Green Fluorescent Proteins
keywords: Gossypium; UDP-GlcA; UDP-glucose dehydrogenase; fiber