title: Determination of Bile Acids in Piglet Bile by Solid Phase Extraction and Liquid Chromatography-Electrospray Tandem Mass Spectrometry.
abstract: An LC/MS/MS-based method was developed for the determination of individual bile acids (BA) and their conjugates in porcine bile samples. The C18-based solid-phase extraction (SPE) procedure was optimized so that all 19 target BA and their glycine and taurine conjugates were collected with high recoveries for standards (89.1-100.2%). Following this, all 19 compounds were separated and quantified in a single 12 min chromatographic run. The method was validated in terms of linearity, sensitivity, accuracy, precision, and recovery. An LOD in the low ppb range with measured precisions in the range of 0.5-9.3% was achieved. The recoveries for all of the 19 analytes in bile samples were all >80%. The validated method was successfully applied to the profiling of BA and their conjugates in the bile from piglets treated with exogenous glucagon-like peptide-2 (GLP-2) in a preclinical model of neonatal parenteral nutrition-associated liver disease (PNALD). The method developed is rapid and could be easily implemented for routine analysis of BA and their conjugates in other biofluids or tissues.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, 3-60D South Academic Building, Edmonton, AB, T6G 2P5, Canada.
authors.forename: Si
authors.initials: S
authors.lastname: Mi
authors.affiliation: Department of Surgery, University of Alberta, Edmonton, AB, Canada.
authors.forename: David W
authors.initials: DW
authors.lastname: Lim
authors.affiliation: Department of Pediatrics, University of Alberta, Edmonton, AB, Canada.
authors.forename: Justine M
authors.initials: JM
authors.lastname: Turner
authors.affiliation: Department of Surgery, Hospital for Sick Children and University of Toronto, Toronto, ON, Canada.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wales
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, 3-60D South Academic Building, Edmonton, AB, T6G 2P5, Canada. jonathan.curtis@ales.ualberta.ca.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Curtis
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D011786:Quality Control; D052616:Solid Phase Extraction; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry
chemical_list: D001647:Bile Acids and Salts
keywords: Bile acids; GLP-2; LC-ESI-MS/MS; PNALD; Piglet bile; SPE; Validation
references.citation: J Lipid Res. 2001 Jan;42(1):114-9
references.pmid: 11160372
references.citation: Anal Chem. 2010 Jun 15;82(12):5282-9
references.pmid: 20469835
references.citation: Life Sci. 1991;49(18):1301-8
references.pmid: 1833605
references.citation: Anal Biochem. 2011 Jan 15;408(2):242-52
references.pmid: 20875389
references.citation: World J Gastroenterol. 2009 Feb 21;15(7):804-16
references.pmid: 19230041
references.citation: JPEN J Parenter Enteral Nutr. 2016 Jan;40(1):14-21
references.pmid: 25280755
references.citation: J Proteome Res. 2009 May;8(5):2495-500
references.pmid: 19253995
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jun 6;837(1-2):11-7
references.pmid: 16651035
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15;871(2):306-13
references.pmid: 18511357
references.citation: JPEN J Parenter Enteral Nutr. 2016 Jan;40(1):22-35
references.pmid: 26220199
references.citation: Steroids. 2012 Jun;77(7):745-55
references.pmid: 22521565
references.citation: J Chromatogr A. 2013 Jul 19;1299:103-9
references.pmid: 23768533
references.citation: Lipids. 2002 Nov;37(11):1093-100
references.pmid: 12558060
references.citation: J Lipid Res. 1978 May;19(4):510-3
references.pmid: 659989
references.citation: World J Gastroenterol. 2012 Mar 14;18(10):1067-76
references.pmid: 22416181
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 1;873(2):209-17
references.pmid: 18801708
references.citation: Lipids. 2004 Aug;39(8):703-22
references.pmid: 15638239
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Sep 1;878(25):2307-13
references.pmid: 20674519