title: The inhibitory effect of soybean and soybean isoflavone diets on 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice.
abstract: Murine contact hypersensitivity (CHS) is one of the most frequently used animal models of human allergic contact dermatitis. We investigated the inhibitory effects of soybean and soy isoflavone (SI) diets on 2,4-dinitrofluorobenzene- (DNFB) induced CHS in mice. The DNFB-induced ear swelling was inhibited in the soy- and SI-treated groups. Histopathological investigations revealed that oral feeding of soybean and SI attenuated ear tissue edema and reduced the number of Gr-1(+) cell infiltrations into ear tissues. DNA microarray analysis showed that the expression of Ccl24, Xcl1, Ifng, and Ccl17 in the ear tissues was lower in the soy-treated mice than in the positive controls. In addition, CCL24 mRNA and protein expression in the ear tissues were more highly suppressed in the soy- and SI-treated groups. These results suggest that soybean and SI consumption downregulated the gene and protein expression of CCL24, thereby affording protection against CHS in mice.
authors.affiliation: a Department of Clinical Nutrition, Faculty of Health Science and Technology , Kawasaki University of Medical Welfare , Okayama , Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Nagano
authors.affiliation: a Department of Clinical Nutrition, Faculty of Health Science and Technology , Kawasaki University of Medical Welfare , Okayama , Japan.
authors.forename: Woruna
authors.initials: W
authors.lastname: Wu
authors.affiliation: b Fuji Oil Co., Ltd. , Osaka , Japan.
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Tsumura
authors.affiliation: b Fuji Oil Co., Ltd. , Osaka , Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yonemoto-Yano
authors.affiliation: c Division of Gastroenterology, Department of Internal Medicine , Kawasaki Medical School , Okayama , Japan.
authors.forename: Tomoari
authors.initials: T
authors.lastname: Kamada
authors.affiliation: c Division of Gastroenterology, Department of Internal Medicine , Kawasaki Medical School , Okayama , Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D054414:Chemokine CCL17 / Q000235:genetics / Q000276:immunology; D054423:Chemokine CCL24 / Q000235:genetics / Q000276:immunology; D019744:Chemokines, C / Q000235:genetics / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000178:diet therapy* / Q000209:etiology / Q000276:immunology / Q000473:pathology; D004032:Diet; D004139:Dinitrofluorobenzene / Q000633:toxicity; D004195:Disease Models, Animal; D004423:Ear / Q000098:blood supply / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000178:diet therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007529:Isoflavones / Q000008:administration & dosage* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D013025:Glycine max / Q000737:chemistry*
chemical_list: D018926:Anti-Allergic Agents; C499538:Ccl17 protein, mouse; C515485:Ccl24 protein, mouse; D054414:Chemokine CCL17; D054423:Chemokine CCL24; D019744:Chemokines, C; C503133:Gr-1 protein, mouse; C000611649:IFNG protein, mouse; D007529:Isoflavones; D019707:Receptors, Chemokine; C486249:Xcl1 protein, mouse; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene
keywords: chemokine (C-C motif) ligand 24; contact hypersensitivity; isoflavones; soybean