title: Evaluation of Limited Sampling Methods for Oral Busulfan Pharmacokinetic Monitoring in Adult Patients Undergoing Hematopoietic Stem Cell Transplantation.
abstract: Monitoring busulfan area under the plasma concentration-time curve (AUC) to establish the dose regimen for stem cell transplantation desirable to achieve efficacy while avoiding toxicity.
  
  
  
  Our objective was to compare AUCs calculated by 18 limited sampling methods (LSMs) from 2 to 5 samples to reference AUCs determined from 10 samples in a retrospective study of 103 adult patients receiving oral busulfan. LSMs using 2 or 3 samples were ineffective.
  
  
  
  Four LSMs using 4 or 5 blood samples that accurately characterized busulfan AUC were identified. The best 2 methods were obtained with sampling at 0.5, 1, 2, 4, and 6 hours and after 1, 1.5, 2, 4, and 6 hours postdose. For these LSMs, the incidence of 20% difference between AUCs from LSMs and reference AUCs was less than 1.3%.
  
  
  
  Effective and safe determination of AUC for oral busulfan can be made with strategies using only 4 or 5 concentration timepoints.
authors.affiliation: *Department of Pharmacy, Hôpital Maisonneuve-Rosemont; †Faculty of Pharmacy, Université de Montréal; ‡CRCHUM, Centre hospitalier de l'Université de Montréal, Quebec, Canada.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouchard
authors.forename: Sarah
authors.initials: S
authors.lastname: Bilodeau
authors.forename: Karine
authors.initials: K
authors.lastname: Alain
authors.forename: Barbara
authors.initials: B
authors.lastname: Vadnais
authors.forename: Martin
authors.initials: M
authors.lastname: Franco
authors.forename: Jacques
authors.initials: J
authors.lastname: Turgeon
authors.forename: Veronique
authors.initials: V
authors.lastname: Michaud
mesh_terms: D000284:Administration, Oral; D000328:Adult; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002066:Busulfan / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D016903:Drug Monitoring / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D012189:Retrospective Studies; D013997:Time Factors
chemical_list: D018906:Antineoplastic Agents, Alkylating; D002066:Busulfan