title: The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis.
abstract: Heterologous biosynthesis of natural products is meant to enable access to the vast array of valuable properties associated with these compounds. Often motivated by limitations inherent in native production hosts, the heterologous biosynthetic process begins with a candidate host regarded as technically advanced relative to original producing organisms. Given this requirement, E. coli has been a top choice for heterologous biosynthesis attempts as associated recombinant tools emerged and continue to develop. However, success requires overcoming challenges associated with natural product formation, including complex biosynthetic pathways and the need for metabolic support. These two challenges have been heavily featured in cellular engineering efforts completed to position E. coli as a viable surrogate host. This chapter outlines steps taken to engineer E. coli with an emphasis on genetic manipulations designed to support the heterologous production of polyketide, nonribosomal peptide, and similarly complex natural products.
authors.affiliation: Department of Chemical and Biological Engineering, The State University of New York at Buffalo, 904 Furnas Hall, Buffalo, NY, 14260, USA.
authors.forename: Haoran
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biological Engineering, The State University of New York at Buffalo, 904 Furnas Hall, Buffalo, NY, 14260, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.affiliation: EarthGenes Pharmaceuticals, Lexington, MA, 02421, USA.|Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, 02111, USA.
authors.forename: Marcia S
authors.initials: MS
authors.lastname: Osburne
authors.affiliation: Department of Chemical and Biological Engineering, The State University of New York at Buffalo, 904 Furnas Hall, Buffalo, NY, 14260, USA. blainepf@buffalo.edu.
authors.forename: Blaine A
authors.initials: BA
authors.lastname: Pfeifer
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001688:Biological Products / Q000378:metabolism*; D053898:Biosynthetic Pathways; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D043586:Methylmalonyl-CoA Decarboxylase / Q000235:genetics / Q000378:metabolism; D009876:Operon; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D061065:Polyketides / Q000378:metabolism*; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001688:Biological Products; D029968:Escherichia coli Proteins; D061065:Polyketides; C067405:TrfA protein, E coli; D011938:Rec A Recombinases; D043586:Methylmalonyl-CoA Decarboxylase
keywords: Cellular design; E. coli; Heterologous biosynthesis; Natural products; Nonribosomal peptide; Polyketide
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