title: Repair of rabbit cartilage defect based on the fusion of rabbit bone marrow stromal cells and Nano-HA/PLLA composite material.
abstract: Objective To assess the effect of the fusion of rabbit bone marrow stromal cells (rBMSCs) and Nano-hydroxyapatite/poly (l-lactic acid) (Nano-HA/PLLA) in repairing the rabbit knee joint with full-thickness cartilage defect. Method The rBMSCs were isolated and cultured in vitro, and the third generation of rBMSCs was co-cultured with the Nano-HA/PLLA to construct the tissue-engineered cartilage (TEC). Eighteen New Zealand white rabbits were selected and randomly divided into three groups, namely, TEC group, Nano-HA/PLLA group, and control group. A cartilage defect model with the diameter of 4.5 mm and depth of 5 mm was constructed on the articular surface of medial malleolus of rabbit femur. General observation, histological observation, and Wakitani's histological scoring were conducted in the 12th and 24th week postoperatively. Results The results of TEC group indicated that new cartilage tissue was formed on the defect site and subchondral bone achieved physiological integration basically. Histological and immunohistochemical analyses indicated the generation of massive extracellular matrix. In contrast, limited regeneration and reconstruction of cartilage was achieved in the Nano-HA/PLLA group and control group, with a significant difference from the TEC group (p < 0.05). Moreover, the effect of cartilage repair was positively correlated with time. Conclusion The porous Nano-HA/PLLA combined with BMSCs promoted the repair of weight-bearing bone of adult rabbit's knee joint with cartilage defect.
authors.affiliation: a Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , PR China.|b Guangzhou Medical University , Guangzhou , PR China.|c Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Shenzhen University , Shenzhen , PR China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: a Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , PR China.
authors.forename: Daiqi
authors.initials: D
authors.lastname: Guo
authors.affiliation: a Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , PR China.
authors.forename: Liangquan
authors.initials: L
authors.lastname: Peng
authors.affiliation: b Guangzhou Medical University , Guangzhou , PR China.
authors.forename: Yun Fang
authors.initials: YF
authors.lastname: Chen
authors.affiliation: b Guangzhou Medical University , Guangzhou , PR China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Cui
authors.affiliation: c Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Shenzhen University , Shenzhen , PR China.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Xiong
authors.affiliation: c Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Shenzhen University , Shenzhen , PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: c Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Shenzhen University , Shenzhen , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.affiliation: c Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Shenzhen University , Shenzhen , PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Chen
authors.affiliation: d Biomechanics and Medical Information Institute, Beijing University of Technology , Beijing , PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: a Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , PR China.|c Department of Sports Medical , the Affiliated Clinical College Shenzhen Second People's Hospital, Shenzhen University , Shenzhen , PR China.
authors.forename: Daping
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D007718:Knee Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D007719:Knee Joint / Q000378:metabolism* / Q000473:pathology; D008297:Male; D053761:Nanocomposites / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011817:Rabbits; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D011091:Polyesters; C033616:poly(lactide); D017886:Durapatite
keywords: Bone marrow stromal cells; porous composite scaffold material; tissue-engineered cartilage