title: Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome.
abstract: Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that IE1-CTD specifically interacts with the H2A-H2B acidic patch and impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Qianglin
authors.initials: Q
authors.lastname: Fang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Fang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Li
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Rui-Ming
authors.identifier: http://orcid.org/0000-0002-6537-4623
authors.initials: RM
authors.lastname: Xu
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D003587:Cytomegalovirus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry* / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002843:Chromatin; C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; D009707:Nucleosomes
keywords: biochemistry; biophysics; chromatin; crystal structure; cytomegalovirus; human; nucleosome; structural biology; virus
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