title: Histone deacetylase 1 is required for the development of the zebrafish inner ear.
abstract: Histone deacetylase 1 (HDAC1) has been reported to be important for multiple aspects of normal embryonic development, but little is known about its function in the development of mechanosensory organs. Here, we first confirmed that HDAC1 is expressed in the developing otic vesicles of zebrafish by whole-mount in situ hybridization. Knockdown of HDAC1 using antisense morpholino oligonucleotides in zebrafish embryos induced smaller otic vesicles, abnormal otoliths, malformed or absent semicircular canals, and fewer sensory hair cells. HDAC1 loss of function also caused attenuated expression of a subset of key genes required for otic vesicle formation during development. Morpholino-mediated knockdown of HDAC1 resulted in decreased expression of members of the Fgf family in the otic vesicles, suggesting that HDAC1 is involved in the development of the inner ear through regulation of Fgf signaling pathways. Taken together, our results indicate that HDAC1 plays an important role in otic vesicle formation.
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, P.R. China.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: He
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, P.R. China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Tang
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, P.R. China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.affiliation: Co-innovation Center of Neuroregeneration, Key Laboratory for Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing, Jiangsu, 210096, P.R. China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Chai
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, P.R. China.|Key Laboratory of Hearing Medicine of National Health and Family Planning Commission, Shanghai, P.R. China.|Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, P.R. China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D007758:Ear, Inner / Q000196:embryology*; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins; D005346:Fibroblast Growth Factors; C488363:HDAC1 protein, zebrafish; D056284:Histone Deacetylase 1
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