title: Effective Synthesis of Fluorescently Labeled Morpholino Nucleoside Triphosphate Derivatives.
abstract: Morpholino nucleoside triphosphates (A, U, G, C, T) bearing the active functional amino group tethered to morpholine residue and their fluorescently labeled derivatives were synthesized. All compounds were characterized by (1)H, (13)C, and (31)P NMR, and mass spectrometry. A possibility of using fluorescently labeled morpholino nucleoside triphosphates as chain terminators in DNA sequencing is discussed.
authors.affiliation: a Institute of Chemical Biology and Fundamental Medicine SB RAS , Lavrent'ev Ave 8, Novosibirsk 630090 , Russian Federation.
authors.forename: Yuliya V
authors.initials: YV
authors.lastname: Tarasenko
authors.affiliation: a Institute of Chemical Biology and Fundamental Medicine SB RAS , Lavrent'ev Ave 8, Novosibirsk 630090 , Russian Federation.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Abramova
authors.affiliation: b Institute of Organic Chemistry SB RAS , Lavrent'ev Ave 9, Novosibirsk 630090 , Russian Federation.
authors.forename: Viktor I
authors.initials: VI
authors.lastname: Mamatuk
authors.affiliation: a Institute of Chemical Biology and Fundamental Medicine SB RAS , Lavrent'ev Ave 8, Novosibirsk 630090 , Russian Federation.|c NanoTech-S LLS , Lavrent'ev Ave 8, Novosibirsk 630090 , Russian Federation.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Silnikov
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry*; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009025:Morpholines; D009711:Nucleotides
keywords: Morpholino nucleosides; fluorescent labeling; triphosphates