title: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer.
abstract: Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. It was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.
authors.affiliation: Chemical Engineering, Louisiana Tech University, 600 W. Arizona, Ruston, LA USA.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Mechanical Engineering, Louisiana Tech University, 600 W. Arizona, Ruston, LA USA.
authors.forename: Hisham E
authors.initials: HE
authors.lastname: Hegab
authors.affiliation: Chemistry, Louisiana Tech University, 600 W. Arizona, Ruston, LA USA.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Lvov
authors.affiliation: Chemistry, Louisiana Tech University, 600 W. Arizona, Ruston, LA USA.
authors.forename: L
authors.initials: L
authors.lastname: Dale Snow
authors.affiliation: Chemical Engineering, Louisiana Tech University, 600 W. Arizona, Ruston, LA USA.
authors.forename: James
authors.initials: J
authors.lastname: Palmer
keywords: (3-Aminopropyl) triethoxy-silane; Cellulase; Glucose; Glutaraldehyde; Silica gel
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