title: JadX is a Disparate Natural Product Binding Protein.
abstract: We report that JadX, a protein of previously undetermined function coded for in the jadomycin biosynthetic gene cluster of Streptomyces venezuelae ISP5230, affects both chloramphenicol and jadomycin production levels in blocked mutants. Characterization of recombinant JadX through protein-ligand interactions by chemical shift perturbation and WaterLOGSY NMR spectroscopy resulted in the observation of binding between JadX and a series of jadomycins and between JadX and chloramphenicol, another natural product produced by S. venezuelae ISP5230. These results suggest JadX to be an unusual class of natural product binding protein involved in binding structurally disparate natural products. The ability for JadX to bind two different natural products in vitro and the ability to affect production of these secondary metabolites in vivo suggest a potential role in regulation or signaling. This is the first example of functional characterization of these JadX-like proteins, and provides insight into a previously unobserved regulatory process.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Robertson
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Forget
authors.forename: Camilo F
authors.initials: CF
authors.lastname: Martinez-Farina
authors.forename: Nicole E
authors.initials: NE
authors.lastname: McCormick
authors.affiliation: Institute for Marine Biosciences, National Research Council of Canada , Halifax, Nova Scotia B3H 3Z1, Canada.
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Syvitski
authors.forename: David L
authors.initials: DL
authors.lastname: Jakeman
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002701:Chloramphenicol / Q000737:chemistry / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry / Q000378:metabolism; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001688:Biological Products; D002352:Carrier Proteins; D007546:Isoquinolines; D011994:Recombinant Proteins; C000609367:jadomycin DS; D002701:Chloramphenicol