title: The Impact of Simulated Weightlessness on Endothelium-Dependent Angiogenesis and the Role of Caveolae/Caveolin-1.
abstract: OBJECTIVE
  The potential role of caveolin-1 in modulating angiogenesis in microgravity environment is unexplored.
  
  
  METHODS
  Using simulated microgravity by clinostat, we measured the expressions and interactions of caveolin-1 and eNOS in human umbilical vein endothelial cells.
  
  
  RESULTS
  We found that decreased caveolin-1 expression is associated with increased expression and phosphorylation levels of eNOS in endothelial cells stimulated by microgravity, which causes a dissociation of eNOS from caveolin-1 complexes. As a result, microgravity induces cell migration and tube formation in endothelial cell in vitro that depends on the regulations of caveolin-1.
  
  
  CONCLUSIONS
  Our study provides insight for the important endothelial functions in altered gravitational environments.
authors.affiliation: Department of Aerospace Biodynamics, Faculty of Aerospace Medicine, The Fourth Military Medical University, Xi'an, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
authors.forename: Tian-Zhi
authors.initials: TZ
authors.lastname: Zhao
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Wang
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Cao
authors.forename: Chang-Bin
authors.initials: CB
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Cheng-Fei
authors.initials: CF
authors.lastname: Li
authors.forename: Jiang-Dong
authors.initials: JD
authors.lastname: Zhao
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Xi-Qing
authors.initials: XQ
authors.lastname: Sun
mesh_terms: D021941:Caveolae / Q000378:metabolism*; D051242:Caveolin 1 / Q000032:analysis / Q000378:metabolism*; D002465:Cell Movement; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018919:Neovascularization, Physiologic; D052250:Nitric Oxide Synthase Type III / Q000032:analysis / Q000378:metabolism*; D060066:Protein Interaction Maps; D018474:Weightlessness Simulation
chemical_list: D051242:Caveolin 1; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III