title: [SYNTHESIS OF PHYTOHORMONES BY NOCARDIA VACCINII IMV B-7405--PRODUCER OF SURFACTANTS].
abstract: OBJECTIVE
  To study the synthesis of phytohormones (auxins, cytokinins, abscisic acid) under cultivation of Nocardia vaccinii IMV B-7405 (surfactants producer) in media containing different carbon sources (glycerol, refined sunflower oil, as well as waste oil after frying potatoes and meat).
  
  
  METHODS
  Phytohormones were extracted from supernatants of culture liquid (before or after surfactant separation) by ethylacetate (auxins, abscisic acid) and n-butanol (cytokinins), concentrated and purified by thin-layer chromatography, then quantitative determination was performed using a scanning Sorbfil spectrodensitometer.
  
  
  RESULTS
  While growing in medium with refined oil IMV B-7405 strain synthesized 1.8 ± 0.09 g/l extracellular surfactant, also maximum amount of auxins (245-770 µ/l) and cytokinins (134-348 µl). Cultivation of N. vaccini LMV B-7405 on waste oil was accompanied by decreasing amount of phytohormones to 23-84 µ/l (auxins) and 16-90 µ/l (cytokinins) and increasing surfactant concentration to 2.3-2.6 g/l. The level of abscisic acid synthesis was practically not dependent on the nature of growth substrate, was substantially lower than that of auxins and cytokinins and ranged from 2 to 12 µ/l.
  
  
  CONCLUSIONS
  Obtained data demonstrate the possibility of using oil-containing industrial waste for the simultaneous synthesis of both surfactants and phytohormones, and indicate the need for studies of the effect of producer cultivation conditions on the biological properties of the target products of microbial synthesis.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: N O
authors.initials: NO
authors.lastname: Leonova
authors.forename: T A
authors.initials: TA
authors.lastname: Shevchuk
authors.forename: E V
authors.initials: EV
authors.lastname: Panasuk
authors.forename: K A
authors.initials: KA
authors.lastname: Beregovaya
authors.forename: G A
authors.initials: GA
authors.lastname: Iutynskaya
mesh_terms: D020001:1-Butanol; D000040:Abscisic Acid / Q000096:biosynthesis* / Q000302:isolation & purification; D000085:Acetates; D003470:Culture Media / Q000737:chemistry; D003583:Cytokinins / Q000096:biosynthesis* / Q000302:isolation & purification; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D007210:Indoleacetic Acids / Q000302:isolation & purification / Q000378:metabolism*; D007218:Industrial Microbiology; D007219:Industrial Oils / Q000032:analysis; D007220:Industrial Waste / Q000032:analysis; D009615:Nocardia / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000302:isolation & purification / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism; D012997:Solvents; D000074242:Sunflower Oil; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000085:Acetates; D003470:Culture Media; D003583:Cytokinins; D007210:Indoleacetic Acids; D007220:Industrial Waste; D010937:Plant Growth Regulators; D010938:Plant Oils; D012997:Solvents; D000074242:Sunflower Oil; D013501:Surface-Active Agents; D000040:Abscisic Acid; C007650:ethyl acetate; D020001:1-Butanol; D005990:Glycerol