title: Revisiting the neural role of estrogen receptor beta in male sexual behavior by conditional mutagenesis.
abstract: Estradiol derived from neural aromatization of gonadal testosterone plays a key role in the perinatal organization of the neural circuitry underlying male sexual behavior. The aim of this study was to investigate the contribution of neural estrogen receptor (ER) β in estradiol-induced effects without interfering with its peripheral functions. For this purpose, male mice lacking ERβ in the nervous system were generated. Analyses of males in two consecutive tests with a time interval of two weeks showed an effect of experience, but not of genotype, on the latencies to the first mount, intromission, pelvic thrusting and ejaculation. Similarly, there was an effect of experience, but not of genotype, on the number of thrusts and mating length. Neural ERβ deletion had no effect on the ability of males to adopt a lordosis posture in response to male mounts, after castration and priming with estradiol and progesterone. Indeed, only low percentages of both genotypes exhibited a low lordosis quotient. It also did not affect their olfactory preference. Quantification of tyrosine hydroxylase- and kisspeptin-immunoreactive neurons in the preoptic area showed unaffected sexual dimorphism of both populations in mutants. By contrast, the number of androgen receptor- and ERα-immunoreactive cells was significantly increased in the bed nucleus of stria terminalis of mutant males. These data show that neural ERβ does not play a crucial role in the organization and activation of the neural circuitry underlying male sexual behavior. These discrepancies with the phenotype of global ERβ knockout models are discussed.
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
authors.forename: Lydie
authors.initials: L
authors.lastname: Naulé
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Marie-Luce
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Parmentier
authors.affiliation: UMR 85, Institut National de la Recherche Agronomique, Nouzilly, France; UMR7247, Centre National de la Recherche Scientifique, Nouzilly, France; Université François Rabelais, Tours, France; Institut Français du Cheval et de l'Equitation, Nouzilly, France.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Martini
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Albac
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
authors.forename: Anne-Charlotte
authors.initials: AC
authors.lastname: Trouillet
authors.affiliation: UMR 85, Institut National de la Recherche Agronomique, Nouzilly, France; UMR7247, Centre National de la Recherche Scientifique, Nouzilly, France; Université François Rabelais, Tours, France; Institut Français du Cheval et de l'Equitation, Nouzilly, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Keller
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Hardin-Pouzet
authors.affiliation: Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France. Electronic address: sakina.mhaouty-kodja@upmc.fr.
authors.forename: Sakina
authors.initials: S
authors.lastname: Mhaouty-Kodja
mesh_terms: D000818:Animals; D002872:Chromosome Deletion; D047629:Estrogen Receptor beta / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000235:genetics; D007032:Hypothalamus, Anterior / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016296:Mutagenesis / Q000235:genetics*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011247:Pregnancy; D011301:Preoptic Area / Q000502:physiology; D012686:Septal Nuclei / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000502:physiology*
chemical_list: D047629:Estrogen Receptor beta
keywords: Conditional mutagenesis; Estradiol; Estrogen receptor beta; Male reproduction; Nervous system; Sex steroid hormones; Sexual behavior; Sexual dimorphism