title: Polysaccharides from Rhizoma Panacis Majoris and its anti-oxidant activity.
abstract: Response surface method (RSM) was employed to optimize the extraction conditions of polysaccharides from Rhizoma Panacis Majoris (the rhizomes of Panax japonicus C. A. Mey. var. major (Burk.) C. Y. Wu et K. M. Feng) (RPMP), a well-known Chinese traditional medicine. In order to obtain the optimal processing parameters, a three-variable Box-Behnken designs (BBD) were applied for experimental designs. RSM analysis indicated the good correspondence between experimental and predicted values, the optimal conditions for the yield of polysaccharides were as follows: the ultrasound time is 31.15 min, extraction temperature is 92.50 °C, and the ratio of water to raw material is 40 mL/g. The maximum value (13.87 ± 0.16%) of the yield of polysaccharides was obtained under these optimal conditions. The molecular weight (MW) was determined to be 1.48 × 10(5)(±0.39%)Da by HPSEC-MALLS-RID chromatography system. FT-IR spectra demonstrated obvious characteristic peaks of polysaccharides. The antioxidant activities of RPMP were investigated including scavenging activity of hydrogen radicals, ABTS radicals, and free radicals of superoxide anion in vitro, and the results exhibited that RPMP had a good potential for antioxidant.
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Rufeng
authors.initials: R
authors.lastname: Wang
authors.affiliation: Hubei Institute for Food and Drug Control, Wuhan 430064, China.
authors.forename: Danping
authors.initials: D
authors.lastname: Li
authors.affiliation: Hubei Institute for Food and Drug Control, Wuhan 430064, China.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: chenpingvip24@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
mesh_terms: D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification*; D005609:Free Radicals / Q000737:chemistry; D008970:Molecular Weight; D005894:Panax / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides
keywords: Antioxidant activity; Polysaccharide; Rhizoma Panacis Majoris