title: Self-Assembly-Assisted Biomolecule-Enriched Surface and High Selectivity Performance of Simple Solution-Coatable Biomimicking Brush Copolymers.
abstract: Poly(oxy(11-(biotinyl)undecylthiomethyl)ethylene-co-oxy(11-phosphoryl-cholineundecylthiomethyl)ethylene)s (PECH-BTmPCn: m = 0-100 mol % biotin (BT)-containing bristle; n = 100-0 mol % phosphorylcholine (PC)-containing bristle) were newly synthesized. All polymers exhibited excellent solution processability. They favorably self-assembled horizontal multibilayer structures in thin films with BT- and PC-enriched surfaces, which were driven by the lateral ordering of the fully extended upright bristles and the partial interdigitation between the BT and PC end groups of the bristles. Both hydrophilicity and water sorption of the films increased with the PC content. The PECH-BT100 films revealed remarkably distinctive sensitivity, selectivity, and adsorption ability for avidin against other proteins. Such remarkable performance was further significantly enhanced on the PECH-BTmPCn films in which PC moieties were incorporated to the BT-rich surface; in particular, the PECH-BT75PC25 films demonstrated the highest performance. Overall, the self-assembly brush copolymers of this study are very suitable for use in the high performance detection, adsorption, and separation of proteins and receptors, including avidin, which can reveal high affinity and selectivity to BT moiety.
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang Accelerator Laboratory, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology , Pohang 790-784, Republic of Korea.
authors.forename: Kyungho
authors.initials: K
authors.lastname: Kwon
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang Accelerator Laboratory, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology , Pohang 790-784, Republic of Korea.
authors.forename: Changsub
authors.initials: C
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang Accelerator Laboratory, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology , Pohang 790-784, Republic of Korea.
authors.forename: Jongchan
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Dongguk Medical Institute, Dongguk University College of Medicine , Gyeongju 780-714, Republic of Korea.
authors.forename: Heesoo
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang Accelerator Laboratory, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology , Pohang 790-784, Republic of Korea.
authors.forename: Moonhor
authors.initials: M
authors.lastname: Ree
mesh_terms: D000327:Adsorption; D001360:Avidin / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D012680:Sensitivity and Specificity
chemical_list: D011108:Polymers; C000612954:poly(oxy(11-(biotinyl)undecylthiomethyl)ethylene-co-oxy(11-phosphorylcholineundecylthiomethyl)ethylene); D010767:Phosphorylcholine; D001360:Avidin; D011092:Polyethylene Glycols; D001710:Biotin