title: Analyses of Long Non-Coding RNA and mRNA profiling using RNA sequencing during the pre-implantation phases in pig endometrium.
abstract: Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated the expression profiles of endometrial tissue on Days 9, 12 and 15 of pregnancy and on Day 12 of non-pregnancy in Yorkshire, and performed a comprehensive analysis of long non-coding RNAs (lncRNAs) in endometrial tissue samples by using RNA sequencing. As a result, 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison. In agreement with previous reports, lncRNAs shared similar characteristics, such as shorter in length, lower in exon number, lower at expression level and less conserved than protein coding transcripts. Bioinformatics analysis showed that DEGs were involved in protein binding, cellular process, immune system process and enriched in focal adhesion, Jak-STAT, FoxO and MAPK signaling pathway. We also found that lncRNAs TCONS_01729386 and TCONS_01325501 may play a vital role in embryo pre-implantation. Furthermore, the expression of FGF7, NMB, COL5A3, S100A8 and PPP1R3D genes were significantly up-regulated at the time of maternal recognition of pregnancy (Day 12 of pregnancy). Our results first identified the characterization and expression profile of lncRNAs in pig endometrium during pre-implantation phases.
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Songyi
authors.initials: S
authors.lastname: Xue
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: National Animal Husbandry Services Ministry of Agriculture, Beijing, PR China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong (Central China) Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Minggang
authors.initials: M
authors.lastname: Lei
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D011247:Pregnancy / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger
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