title: Epithelial cells from oral mucosa: How to cultivate them?
abstract: Epithelial cells from oral mucosa (EOM) are responsible for important functions, like the primary protection of oral mucosa against external aggressions building a mechanical barrier against microorganisms, mechanical damage, toxic material, thermal regulation and secretion of different classes of inflammatory mediators. EOM could be an interesting tool for cellular and molecular biology research. Usually, EOM are collected by a painful and invasive process. In this study, we propose an alternative method to cultivate EOM collected by non-invasive scraping method of oral mucosa. Papanicolaou staining showed mainly two kinds of epithelial cell population after EOM scraping. As result of the five culture methods tested here, our results revealed that the EOM were successfully cultured on a murine feeder layer. In addition, EOM could be frozen and thawed, without morphology changes and loss of viability. Our findings suggest that EOM can be considered as a good cell source for many purposes, such as genetic studies, diagnosis and cell therapy.
authors.affiliation: Stem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil. fabielerusso@gmail.com.
authors.forename: F B
authors.initials: FB
authors.lastname: Russo
authors.affiliation: Stem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.|Center for Cellular and Molecular Therapy (NETCEM), School of Medicine, University of São Paulo, 455 Dr. Arnaldo Av., São Paulo, 01246-903, Brazil.
authors.forename: G C
authors.initials: GC
authors.lastname: Pignatari
authors.affiliation: Stem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.
authors.forename: I R
authors.initials: IR
authors.lastname: Fernandes
authors.affiliation: Stem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.
authors.forename: J L R M
authors.initials: JL
authors.lastname: Dias
authors.affiliation: Stem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.|Center for Cellular and Molecular Therapy (NETCEM), School of Medicine, University of São Paulo, 455 Dr. Arnaldo Av., São Paulo, 01246-903, Brazil.|Obstetrics Department, School of Arts, Sciences and Humanities, University of São Paulo, 100 Arlindo Béttio Av., São Paulo, 03828-100, Brazil.
authors.forename: P C B
authors.initials: PC
authors.lastname: Beltrão-Braga
keywords: Cell culture; Epithelial cells; Epithelial cells from oral mucosa; Non-invasive method; Oral mucosa
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