title: Stimulatory effect of an algal fucoidan on the release of vascular endothelial tissue-type plasminogen activator as a mechanism of fucoidan-mediated thrombolysis.
abstract: Identifying a pharmacological means for increasing the production of tissue-type plasminogen activator (t-PA) is always desirable to cure impaired production of this enzyme. An algal fucoidan has been shown to exhibit both novel thrombolytic and synergistic stimulatory effects in a mouse thrombosis model. The plasma levels of active t-PA were measured in mouse arterial thrombus models that were treated with various fucoidans to investigate the mechanism of thrombolysis. The mean plasma level of active t-PA after the infusion of fucoidan was 2.136 ± 0.231 ng/ml for nonthrombolytic Fucus fucoidan and 3.917 ± 0.0.529 ng/ml for thrombolytic Undaria fucoidan, which resulted in a 1.56-2.29-fold increase compared with the healthy control group (1.706 ± 0.194 ng/ml) and the untreated thrombus group (2.506 ± 0.301 ng/ml) (P < 0.01). An algal fucoidan has demonstrated to exert a thrombolytic and stimulatory effect via the induction of t-PA release in a dose-dependent manner in an arterial thrombosis model.
authors.affiliation: aDepartment of Radiology bDepartment of Anatomy, School of Medicine, Catholic University of Daegu cDepartment of Thoracic and Cardiovascular Surgery, Samsung Medical Centre, School of Medicine, Sungkyunkwan University, Seoul dDepartment of Biomedical Engineering and Radiology, School of Medicine, Catholic University of Daegu, Daegu, Korea (Republic of).
authors.forename: Soon-Ki
authors.initials: SK
authors.lastname: Min
authors.forename: Sung-Mi
authors.initials: SM
authors.lastname: Han
authors.forename: Jae-Seok
authors.initials: JS
authors.lastname: Jang
authors.forename: Jong-Ki
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005342:Fibrinolysis / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D015912:Thrombolytic Therapy; D013927:Thrombosis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000378:metabolism; D044648:Undaria / Q000737:chemistry*
chemical_list: D005343:Fibrinolytic Agents; D011134:Polysaccharides; C007789:fucoidan; D010959:Tissue Plasminogen Activator