title: Repeated cultivation: non-cell disruption extraction of astaxanthin for Haematococcus pluvialis.
abstract: The operation of cell disruption is indispensable but cost much in microalgae industry. To be simplified, two different reaction mechanisms await in the cell to respond to moderated or stressed environment. The physical and chemical changes of enzyme and turgor pressure of cell in this conversion play an important role in the enhancement of biomass and metabolites. Repeated turgor pressure (based on the structure and mechanics of cell wall) and converted enzyme system (based on photosynthesis) were used to loosen cell wall and then repeated cultivation of Haematococcus pluvialis for astaxanthin extraction was proposed. There was no significant difference of extraction yield between the broken cell (94.75 ± 3.13%) and non-broken cell (92.32 ± 3.24%) treated by the repeated cultivation. Meanwhile, fed-batch culture according to the relationship among pH and nutrient concentration was used to enhance the biomass of Haematococcus pluvialis with the dry cell weight of 1.63 ± 0.07 g/L.
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Han
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Guan
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Geng
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Wang
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D002473:Cell Wall / Q000502:physiology; D000460:Chlorophyta / Q000737:chemistry / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D059828:Plant Cells / Q000378:metabolism; D024341:Xanthophylls / Q000302:isolation & purification
chemical_list: D024341:Xanthophylls; C005948:astaxanthine
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