title: Capturing in Vivo Plant Metabolism by Real-Time Analysis of Low to High Molecular Weight Volatiles.
abstract: We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day-night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, ∼1200 VOCs were monitored displaying different kinetics. To validate the soundness of our in vivo measurements, we fully characterized some key compounds via tandem mass spectrometry (MS/MS) and confirmed their expected behavior based on prior gas chromatography/mass spectrometry (GC/MS) studies. For example, β-caryophyllene, which is directly related to photosynthesis, was found to show a periodic day-night pattern with highest concentrations during the day. We conclude that the capability of SESI-MS to capture highly dynamic VOC emissions and wide analyte coverage makes it an attractive tool to complement GC/MS in plant studies.
authors.affiliation: Department of Energy Engineering and Fluid Dynamics, University of Valladolid , 47002 Valladolid, Spain.|SEADM S.L., 28036 Madrid, Spain.|Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland.
authors.forename: César
authors.initials: C
authors.lastname: Barrios-Collado
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland.
authors.forename: Diego
authors.initials: D
authors.lastname: García-Gómez
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland.
authors.forename: Renato
authors.initials: R
authors.lastname: Zenobi
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland.|Fossil Ion Technology S.L., 28036 Madrid, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Vidal-de-Miguel
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland.|Science Zurich - Zurich PhD Program Molecular Life Sciences, 8093 Zurich, Switzerland.
authors.forename: Alfredo J
authors.initials: AJ
authors.lastname: Ibáñez
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland.
authors.forename: Pablo
authors.initials: P
authors.lastname: Martinez-Lozano Sinues
mesh_terms: D031112:Begoniaceae / Q000737:chemistry* / Q000378:metabolism*; D008027:Light; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation; D013997:Time Factors; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D055549:Volatile Organic Compounds