title: Anti-Proliferative Activity and Apoptosis Induction of an Ethanolic Extract of Boesenbergia pandurata (Roxb.) Schlecht. against HeLa and Vero Cell Lines.
abstract: Rhizomes of Boesenbergia pandurata (Roxb.) Schlecht have been reported to contain active compounds with anticancer properties. This research was carried out to examine anti-proliferative and apoptotic induction against HeLa and Vero cells-line. Dried powder of B. pandurata rhizomes was extracted by a maceration method using 90% ethanol. Cytotoxic assays to determine IC50 and anti-proliferative effects were carried out by MTT methods. Observation of apoptosis was achieved with double staining using acridine orange and ethidium bromide. The results showed that ethanolic extract of B. pandurata was more cytotoxic against HeLa cells (IC50 of 60 μg/ mL) than Vero cells (IC50 of 125 μg/mL). The extract had higher anti-proliferative activity as well as apoptotic induction in HeLa than Vero cells. Therefore, it was concluded that the ethanolic extract of B. pandurata had anti-proliferative as well as apoptosis induction activity dependent on the cell type.
authors.affiliation: Doctoral Program of Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia E-mail : sismindari@ugm.ac.id.
authors.forename: Shanti
authors.initials: S
authors.lastname: Listyawati
authors.lastname: Sismindari
authors.forename: Sofia
authors.initials: S
authors.lastname: Mubarika
authors.forename: Yosi Bayu
authors.initials: YB
authors.lastname: Murti
authors.forename: Muthi
authors.initials: M
authors.lastname: Ikawati
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D000431:Ethanol / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D014709:Vero Cells; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D000431:Ethanol