title: Tumor Suppressor DLEC1 can Stimulate the Proliferation of Cancer Cells When AP-2ɑ2 is Down-Regulated in HCT116.
abstract: BACKGROUND
  The molecular mechanisms of tumor suppressor gene DLEC1 are largely unknown.
  
  
  OBJECTIVE
  In this study, we established DLEC1 over-expression stable clones to study the cellular function of DLEC1 in the colorectal cancer cell line, HCT116.
  
  
  METHODS
  Stable clones with DLEC1 over-expression were first established by the transfection of DLEC1 expression construct pcDNA31DLEC1 in HCT116. On G418 selection, positive stable clones were screened for DLEC1 expression level by conventional reverse transcription-polymerase chain reaction (RT-PCR), and verified by real-time RT-PCR and Western blotting. Subsequently, these stable clones were subjected to colony formation and cell cycle analyses and identification of factors involved in G1 arrest. Lastly, three stable clones, DLEC1-7 (highest DLEC1 expression), DLEC1-3 (lowest expression) and pcDNA31 vector control, were employed to analyze cell proliferation and cell cycle after AP-2α2 knockdown by siRNAs.
  
  
  RESULTS
  The DLEC1 over-expression was found to reduce the number of colonies in colony formation and to induce G1 arrest in seven clones, and apoptosis in one clone in the cell cycle analysis. Furthermore, regardless of the different cell cycle defects in all eight stable clones, the expression level of transcriptional factor AP-2α2 was found to be elevated. More interestingly, we found that when AP-2α2 was knocked down, DLEC1 over-expression neither suppressed cancer cell growth nor induced G1 arrest, yet, instead promoted cell growth and decreased cells in the G1 fraction. This promotion of cell proliferation and release of G1 cells also seemed to be proportional to DLEC1 expression levels in DLEC1 stable clones.
  
  
  CONCLUSIONS
  DLEC1 suppresses tumor cell growth the presence of AP-2α2 and stimulates cell proliferation in the down-regulation of AP-2α2 in DLEC1 over-expression stable clones of HTC116.
authors.affiliation: Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, PR China; Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore, Republic of Singapore.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Qiu
authors.affiliation: Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore, Republic of Singapore.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Xie
authors.affiliation: Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, PR China.
authors.forename: Linhong
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore, Republic of Singapore.
authors.forename: Shing Chuan
authors.initials: SC
authors.lastname: Hooi
keywords: AP-2ɑ; Cell Proliferation; DLEC1; Human; Promotion
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