title: Identification and characterization of Theileria ovis surface protein (ToSp) resembled TaSp in Theileria annulata.
abstract: Ovine theileriosis is an important hemoprotozoal disease of sheep and goats in tropical and subtropical regions which caused high economic loses in the livestock industry. Theileria annulata surface protein (TaSp) was used previously as a tool for serological analysis in livestock. Since the amino acid sequences of TaSp is, at least, in part very conserved in T. annulata, Theileria lestoquardi and Theileria china I and II, it is very important to determine the amino acid sequence of this protein in Theileria ovis as well, to avoid false interpretation of serological data based on this protein in small animal. In the present study, the nucleotide sequence and amino acid sequence of T. ovis surface protein (ToSp) were determined. The comparison of the nucleotide sequence of ToSp showed 96, 96, 99, and 86 % homology to the corresponding nucleotide sequence of TaSp genes by T. annulata, T. China I, T. China II and T. lestoquardi, previously registered in GenBank under accession nos. AJ316260.1, AY274329.1, DQ120058.1, and EF092924.1 respectively. The amino acid sequence analysis showed 95, 81, 98 and 70 % homology to the corresponding amino acid sequence of T. annulata, T chinaI, T china II and T. lestoquardi, registered in GenBank under accession nos. CAC87478.1, AAP36993.1, AAZ30365.1 and AAP36999.11, respectively. Interestingly, in contrast to the C terminus, a significant difference in amino acid sequence in the N teminus of the ToSp protein could be determined compared to the other known corresponding TaSp sequences, which make this region attractive for designing of a suitable tool for serological diagnosis.
authors.affiliation: Center for Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University Of Tehran, Tehran, Iran. pshayan@ut.ac.ir.
authors.forename: P
authors.initials: P
authors.lastname: Shayan
authors.affiliation: Center for Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University Of Tehran, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Jafari
authors.affiliation: Center for Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University Of Tehran, Tehran, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Fattahi
authors.affiliation: Center for Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University Of Tehran, Tehran, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Ebrahimzade
authors.affiliation: Center for Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University Of Tehran, Tehran, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Amininia
authors.affiliation: Department of Parasitology, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Changizi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D008565:Membrane Proteins / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*; D016794:Theileria / Q000145:classification* / Q000235:genetics*; D013801:Theileriasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins
keywords: PCR; Sequencing; TaSp gene; Theileriosis; ToSp gene
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