title: Influence of Dietary Capsaicin on Redox Status in Red Blood Cells During Human Aging.
abstract: OBJECTIVE
  Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is a major pungent compound found in hot peppers of the plant genus Capsicum. In vitro effects of dietary capsaicin on redox status in red blood cells during human aging have been explored.
  
  
  METHODS
  Total antioxidant potential of capsaicin was evaluated using Ferric reducing ability of plasma (FRAP) assay. GSH was measured as per standard protocol. The in vitro effect of capsaicin was evaluated by incubation of the cells in the assay medium with 10(-5)M capsaicin (final concentration) for 60 min at 37°C.
  
  
  RESULTS
  Treatment with capsaicin (10(-5)M) caused a significant (p < 0.01) increase in GSH level in all age groups. Reduced glutathione (GSH) / Oxidized glutathione (GSSG) ratio measures the redox status of the red blood cell. Significant increase in GSH level due to capsaicin, shift the GSH/GSSG ratio, thus alters the redox status of the cell.
  
  
  CONCLUSIONS
  The results conclusively prove the efficacy of the antioxidant property of capsaicin and its role in modulating the redox status of red blood cells. This evidence suggests that dietary factors that act as antioxidants to increase GSH level may contribute to a protective effect against age related diseases. This antioxidant effect may, in part, explain the high consumption of capsicum in certain regions of the world.
authors.affiliation: Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201301, India.
authors.forename: Prabhanshu
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, India.
authors.forename: Subhash
authors.initials: S
authors.lastname: Chand
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India.
authors.forename: Pranjal
authors.initials: P
authors.lastname: Chandra
authors.affiliation: Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201301, India. ; Interdisciplinary Laboratory of Clinical Neuroscience (LINC), Department of Psychiatry, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Pawan Kumar
authors.initials: PK
authors.lastname: Maurya
keywords: Aging; Capsaicin; Erythrocyte; Glutathione; Human; Redox status
references.citation: Indian J Med Res. 2010 May;131:682-91
references.pmid: 20516541
references.citation: Pain Med. 2011 Jan;12(1):99-109
references.pmid: 21087403
references.citation: Cell Death Differ. 1999 Feb;6(2):155-65
references.pmid: 10200562
references.citation: Adv Drug Deliv Rev. 2008 Oct-Nov;60(13-14):1545-52
references.pmid: 18652861
references.citation: Phytother Res. 2006 Apr;20(4):303-6
references.pmid: 16557614
references.citation: Cell Mol Biol Lett. 2003;8(4):919-25
references.pmid: 14668915
references.citation: Indian J Biochem Biophys. 2009 Feb;46(1):130-2
references.pmid: 19374267
references.citation: Rejuvenation Res. 2006 Summer;9(2):169-81
references.pmid: 16706639
references.citation: Adv Pharm Bull. 2014 Oct;4(Suppl 1):443-7
references.pmid: 25364660
references.citation: Eur J Pharmacol. 2013 Nov 15;720(1-3):55-62
references.pmid: 24211679
references.citation: Free Radic Biol Med. 1999 Nov;27(9-10):922-35
references.pmid: 10569625
references.citation: Rejuvenation Res. 2008 Jun;11(3):661-5
references.pmid: 18593284
references.citation: Anal Biochem. 1996 Jul 15;239(1):70-6
references.pmid: 8660627
references.citation: Rejuvenation Res. 2013 Jun;16(3):179-84
references.pmid: 23442131
references.citation: Biochim Biophys Acta. 1995 Mar 22;1234(2):225-34
references.pmid: 7696298