title: Effects of ovariectomy on periodontal tissues following tooth replantation.
abstract: OBJECTIVE
  The aim of the study was to analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation by histomorphometric, immunohistochemistry, and μCT analysis.
  
  
  METHODS
  Eighty wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: ovariectomized (OVX) and Sham. Two months after surgery, the rats' upper right incisor was extracted followed by immediate reimplantation. The animals were sacrificed after 28, 45, and 60 days healing time. Histomorphometric and immunohistochemical analysis were performed by evaluation of PCNA and TRAP straining.
  
  
  RESULTS
  The periodontal ligament was reinserted into the bone and cementum in the both groups. The immunohistochemical analysis revealed PCNA positive cells on the periodontal ligament in both groups at 28 days. Root resorption was noted at 45 days with immunoreactive cells for TRAP present in bone and tooth surface however no statistical differences between the groups were noticed. Histomorphometric analysis showed significant difference between groups in the periodontal ligament and root resorption parameters for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p<0.01), the organization of the periodontal ligament at 28 days (p<0.05), depth of root resorption at 45 days (p<0.05) and at 60 days (p<0.001). The μCT analysis showed multiple areas of bone resorption in association with OVX at 28 and 60 days with no significant differences between times in vivo.
  
  
  CONCLUSIONS
  The ovariectomy did not have significant influence in periodontal tissue parameters following tooth reimplantation.
authors.affiliation: Department of Surgery and Integrated Clinics, Aracatuba Dental School, Univ Estadual Paulista-UNESP, Rua José Bonifácio, 1193-Vila Mendonça, Aracatuba, SP 16015-050, Brazil. Electronic address: heloisafonsecamarao@yahoo.com.br.
authors.forename: Heloisa Fonseca
authors.initials: HF
authors.lastname: Marão
authors.affiliation: Center for Craniofacial Regeneration, College of Dental Medicine, Columbia University, 630 West 168th St., New York, NY 10032, USA.
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Mao
authors.affiliation: Department of Basic Science, Aracatuba Dental School, Univ Estadual Paulista-UNESP, Rodovia Marechal Rondon, KM 527/528, Campus Universitário, Aracatuba, SP 16018-805, Brazil; Biosciences Institute of Botucatu, UNESP Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Cláudio Aparecido
authors.initials: CA
authors.lastname: Casatti
authors.affiliation: Department of Biomaterials and Biomimetics, New York University College of Dentistry, 433 1st Ave., New York, NY 10010, USA; Department of Periodontology and Implant Dentistry, New York University College of Dentistry, 345 E 24th St, New York, NY 10010, USA; Division of Engineering, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates.
authors.forename: Paulo G
authors.initials: PG
authors.lastname: Coelho
authors.affiliation: Department of Basic Science, Aracatuba Dental School, Univ Estadual Paulista-UNESP, Rodovia Marechal Rondon, KM 527/528, Campus Universitário, Aracatuba, SP 16018-805, Brazil.
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
authors.affiliation: Center for Craniofacial Regeneration, College of Dental Medicine, Columbia University, 630 West 168th St., New York, NY 10032, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Surgery and Integrated Clinics, Aracatuba Dental School, Univ Estadual Paulista-UNESP, Rua José Bonifácio, 1193-Vila Mendonça, Aracatuba, SP 16015-050, Brazil.
authors.forename: Vanessa Ferreira
authors.initials: VF
authors.lastname: da Silva
authors.affiliation: Department of Surgery and Integrated Clinics, Aracatuba Dental School, Univ Estadual Paulista-UNESP, Rua José Bonifácio, 1193-Vila Mendonça, Aracatuba, SP 16015-050, Brazil.
authors.forename: Sônia Regina
authors.initials: SR
authors.lastname: Panzarini
mesh_terms: D000818:Animals; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D007150:Immunohistochemistry; D007180:Incisor / Q000473:pathology / Q000601:surgery; D010010:Osteoclasts / Q000166:cytology; D010052:Ovariectomy / Q000379:methods; D010053:Ovary / Q000502:physiology* / Q000601:surgery; D010513:Periodontal Ligament / Q000473:pathology / Q000502:physiology*; D010519:Periodontium / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D012391:Root Resorption / Q000473:pathology; D014090:Tooth Replantation; D014945:Wound Healing / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D004967:Estrogens
keywords: Immunohistochemistry; Micro-ct; Osteoclast; Ovariectomy; Tooth replantation