title: Comparison of the Proteome Profiling of Iranian isolates of Leishmania tropica, L. major and L. infantum by Two-Dimensional Electrophoresis (2-DE) and Mass-spectrometry.
abstract: BACKGROUND
  The mechanisms of virulence and species differences of Leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. In Iran, L. major and L. tropica are known as principal agents of cutaneous leishmaniasis, while L. infantum causes visceral leishmaniasis.
  
  
  METHODS
  As a preliminary study, we compared the proteome mapping of the above three Iranian isolates of Leishmania species through the 2-dimension electrophoresis (2-DE), and identified the prominent proteins by Liquid Chromatography (LC) mass spectrometry.
  
  
  RESULTS
  We reproducibly detected about 700 protein spots in each species by using the Melanie software. Totally, 264 proteins exhibited significant changes among 3 species. Forty nine protein spots identified in both L. tropica and L. major were similar in position in the gel, whereas only 35 of L. major proteins and 10 of L. tropica proteins were matched with those of L. infantum. Having identified 24 proteins in the three species, we sought to provide possible explanations for their differential expression patterns and discuss their relevance to cell biology.
  
  
  CONCLUSIONS
  The comparison of proteome profiling pattern of the 3 species identified limit up and limit down regulated or absent /present proteins. In addition, the LC-MS data analysis showed that most of the protein spots with differential abundance in the 3 species are involved in cell motility and cytoskeleton, cell signaling and vesicular trafficking, intracellular survival / proteolysis, oxidative stress defense, protein synthesis, protein ubiquitination / proteolysis, and stress related proteins. Differentially proteins distributed among the species maybe implicated in host pathogenecity interactions and parasite tropism to cutaneous or visceral tissue macrophages.
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Homa
authors.initials: H
authors.lastname: Hajjaran
authors.affiliation: Agriculture Institute, Iranian Research Organization for Science and Technology, Tehran, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Mohammadi Bazargani
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mohebali
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Science, University of Glasgow, United Kingdom.
authors.forename: Richard
authors.initials: R
authors.lastname: Burchmore
authors.affiliation: Agricultural Biotechnology Research Institute of Iran, (ABRI), Karaj, Iran.
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran; Dept. of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Kazemi-Rad
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, TUMS, Tehran, Iran; Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tums, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Khoramizadeh
keywords: 2-Dimension electrophoresis; L. infantum; L. major; L. tropica; Liquid chromatography; Mass spectrometry; Proteome
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