title: NCR1+ cells appear early in GALT development of the ovine foetus and acquire a c-kit+ phenotype towards the end of gestation.
abstract: The amount, distribution and phenotype of ovine NCR1+ cells were investigated during developing GALT from day 70 of gestation. Antibodies against CD3 and CD79 were used to identify the compartments of GALT, and the localization of NCR1+ cells were correlated within these structures. Markers CD34 and c-kit, in addition to Ki67, were used to investigate possible origin and the stage of development of the NCR1+ cells. NCR1+ cells were present as single cells in the subepithelial tissue as early as 70 days of gestation, and were predominantly present in the T cell rich IFAs and domes as these intestinal wall compartments developed. While NCR1+ cells proliferated more intensively at mid-gestation (70-104 days), the number of NCR1+ cells also expressing c-kit, increased at the end of gestation. In conclusion, NCR1+ cells appeared early in T cell areas of the gut and displayed a phenotype consistent with intermediate stages of cNK cells and/or a subpopulation of ILC22.
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: l.olsen@nmbu.no.
authors.forename: Line
authors.initials: L
authors.lastname: Olsen
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: carolinepiercey.akesson@nmbu.no.
authors.forename: Caroline Piercey
authors.initials: CP
authors.lastname: Åkesson
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: mona.aleksandersen@nmbu.no.
authors.forename: Mona
authors.initials: M
authors.lastname: Aleksandersen
authors.affiliation: Department of Food Safety & Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: preben.boysen@nmbu.no.
authors.forename: Preben
authors.initials: P
authors.lastname: Boysen
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: charles.press@nmbu.no.
authors.forename: Charles McL
authors.initials: CM
authors.lastname: Press
authors.affiliation: Institut National de la Recherche Agronomique, UMR1282, Infectiologie et Santé Publique, Laboratoire Apicomplexes et Immunité Muqueuse, Nouzilly, France. Electronic address: francoise.drouet@tours.inra.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Drouet
authors.affiliation: Department of Food Safety & Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: anne.storset@nmbu.no.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Storset
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: arild.espenes@nmbu.no.
authors.forename: Arild
authors.initials: A
authors.lastname: Espenes
mesh_terms: D000818:Animals; D007413:Intestinal Mucosa / Q000166:cytology / Q000196:embryology; D007422:Intestines / Q000166:cytology / Q000196:embryology*; D016131:Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000196:embryology*; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000096:biosynthesis*; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis*; D012756:Sheep
chemical_list: D055627:Natural Cytotoxicity Triggering Receptor 1; D019009:Proto-Oncogene Proteins c-kit
keywords: Foetus; GALT; Innate lymphoid cells; NCR1; NK cells; Ovine