title: Correlation of interactions between NOS3 polymorphisms and oxygen therapy with retinopathy of prematurity susceptibility.
abstract: OBJECTIVE
  This study was aimed to detect the correlation of nitric oxide synthase 3 (NOS3) gene polymorphisms (T-786C and G894T) and retinopathy of prematurity (ROP) susceptibility. Interaction between NOS3 gene polymorphisms and the duration of oxygen therapy was also explored in ROP babies.
  
  
  METHODS
  Genotypes of NOS3 gene polymorphisms were genotyped by MassArray method. Hardy-Weinberg equilibrium (HWE) was used to calculate the representativeness of the cases and controls. Crossover analysis was utilized to explore the gene environment interactions. Relative risk of ROP was presented by odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs).
  
  
  RESULTS
  Among the subject features, oxygen therapy had obvious difference between case and control groups (P<0.05). There existed significant association between-786C allele and ROP susceptibility (P=0.049, OR=0.669, 95% CI=0.447-0.999). Genotypes of T-786C polymorphism and genotypes and alleles of G894T polymorphism did not related to the susceptibility of ROP. Interactions were existed between NOS3 gene polymorphisms and oxygen therapy duration. When the duration of oxygen therapy was less than 17 days, both -786CC genotype and 894GT genotype were correlated with ROP susceptibility (P=0.020, OR=0.115, 95% CI=0.014-0.960; P=0.011, OR=0.294, 95% CI=0.100-0.784).
  
  
  CONCLUSIONS
  -786C allele might have a protective effect for ROP. Interactions of -786CC and 894GT genotype with oxygen therapy duration (less than 17 days) were both protection factors of ROP.
authors.affiliation: The Children's Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Ophthalmology, The Affiliated Eye Hospital of Nanchang University Nanchang, Jiangxi, China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Yi
authors.affiliation: The Children's Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yin
authors.affiliation: The Children's Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: The Children's Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Nanchang University Nanchang, Jiangxi, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Li
mesh_terms: D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D059647:Gene-Environment Interaction; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016017:Odds Ratio; D010102:Oxygen Inhalation Therapy / Q000009:adverse effects*; D010641:Phenotype; D011110:Polymorphism, Genetic; D065840:Protective Factors; D012178:Retinopathy of Prematurity / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: NOS3 gene; ROP; interaction; oxygen therapy; polymorphisms
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