title: Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
abstract: Among-individual variation in antibody-associated immunity to gastrointestinal nematode parasites (GIN) is known be associated with life-history traits and vital rates in wild vertebrate systems. To date, measurement of levels of antibodies against GIN antigens in natural populations has exclusively been based on invasive blood sampling techniques. Previous work in laboratory rodents and ruminant livestock suggests that antibody measures from feces may provide a viable noninvasive approach. We measured total and anti-GIN antibodies of different isotypes (immunoglobulin (Ig) G, IgA and IgE) from paired samples of plasma and feces from free-living Soay sheep of different ages and sexes. We tested the correlations among these measures as well as their associations with body mass and Strongyle nematode fecal egg counts (FEC). Significant positive correlations were present among plasma and fecal anti-GIN antibody levels for IgG and IgA. Generally, correlations between total antibody levels in plasma and feces were weaker and not significant. No significant relationships were found between any antibody measures and body mass; however, fecal anti-GIN antibody levels were significantly negatively correlated with FEC. Our data clearly demonstrate the feasibility of measuring anti-GIN antibodies from fecal samples collected in natural populations. Although associations of fecal antibody levels with their plasma counterparts and FEC were relatively weak, the presence of significant correlations in the predicted direction in a relatively small and heterogeneous sample suggests fecal antibody measures could be a useful, noninvasive addition to current eco-immunological studies.
authors.affiliation: Institutes of Evolutionary Biology and Immunology and Infection Research School of Biological Sciences University of Edinburgh West Mains Road Edinburgh UK.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Watt
authors.affiliation: Institutes of Evolutionary Biology and Immunology and Infection Research School of Biological Sciences University of Edinburgh West Mains Road Edinburgh UK.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Nussey
authors.affiliation: Institutes of Evolutionary Biology and Immunology and Infection Research School of Biological Sciences University of Edinburgh West Mains Road Edinburgh UK.
authors.forename: Rachel
authors.initials: R
authors.lastname: Maclellan
authors.affiliation: Institutes of Evolutionary Biology and Immunology and Infection Research School of Biological Sciences University of Edinburgh West Mains Road Edinburgh UK.
authors.forename: Jill G
authors.initials: JG
authors.lastname: Pilkington
authors.affiliation: Moredun Research Institute Pentlands Science Park Bush Loan Midlothian UK.
authors.forename: Tom N
authors.initials: TN
authors.lastname: McNeilly
keywords: Fecal egg counts; Ovis aries; Soay sheep; Teladorsagia circumcincta; immunoglobulin; strongyle nematode
references.citation: J Comp Pathol. 1985 Apr;95(2):235-45
references.pmid: 3837794
references.citation: Immunology. 1971 Mar;20(3):277-88
references.pmid: 5551353
references.citation: Cell Host Microbe. 2008 Oct 16;4(4):362-73
references.pmid: 18854240
references.citation: Immunol Rev. 2005 Aug;206:32-63
references.pmid: 16048541
references.citation: Nature. 2005 Feb 17;433(7027):737-41
references.pmid: 15716952
references.citation: Infect Immun. 1998 Jul;66(7):3311-6
references.pmid: 9632600
references.citation: J Immunol Methods. 2004 Oct;293(1-2):153-68
references.pmid: 15541285
references.citation: Parasitology. 1992 Dec;105 ( Pt 3):493-503
references.pmid: 1461688
references.citation: Parasitology. 2006 Oct;133(Pt 4):485-96
references.pmid: 16817995
references.citation: Parasite Immunol. 1995 Dec;17(12):643-52
references.pmid: 8834764
references.citation: Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):160-7
references.pmid: 17544516
references.citation: Parasite Immunol. 2001 May;23(5):227-35
references.pmid: 11309133
references.citation: J Immunol Methods. 2004 Jan;284(1-2):1-6
references.pmid: 14736411
references.citation: Evolution. 2003 Jul;57(7):1670-8
references.pmid: 12940369
references.citation: Parasitology. 2008 Jan;135(Pt 1):71-80
references.pmid: 17903317
references.citation: Front Immunol. 2013 Aug 06;4:222
references.pmid: 23964273
references.citation: Parasitology. 2014 Jun;141(7):875-9
references.pmid: 24534018
references.citation: Allergy. 1992 Dec;47(6):594-8
references.pmid: 1285567
references.citation: Parasite Immunol. 2005 Jun;27(6):213-8
references.pmid: 16048640
references.citation: Vet Parasitol. 1995 Sep;59(2):107-18
references.pmid: 7483234
references.citation: Vaccine. 2008 Dec 9;26(52):6784-90
references.pmid: 18951939
references.citation: J Anim Ecol. 2008 Mar;77(2):370-7
references.pmid: 18028357
references.citation: Proc Biol Sci. 2002 Aug 7;269(1500):1625-32
references.pmid: 12184833
references.citation: J Immunol. 2005 Apr 15;174(8):5024-32
references.pmid: 15814733
references.citation: Infect Immun. 2000 Jul;68(7):3830-9
references.pmid: 10858191
references.citation: J Comp Pathol. 1984 Oct;94(4):591-601
references.pmid: 6512030
references.citation: Vet Immunol Immunopathol. 1983 Mar;4(1-2):43-152
references.pmid: 6223434
references.citation: Immunol Rev. 2014 Jul;260(1):76-85
references.pmid: 24942683
references.citation: Parasitology. 2002 May;124(Pt 5):545-52
references.pmid: 12049417
references.citation: Infect Immun. 1992 May;60(5):2023-9
references.pmid: 1563793
references.citation: J Immunol Methods. 2000 May 26;239(1-2):53-62
references.pmid: 10821947
references.citation: Aging Cell. 2012 Feb;11(1):178-80
references.pmid: 22107028
references.citation: Parasite Immunol. 1993 Feb;15(2):61-7
references.pmid: 8446465
references.citation: Am Nat. 2014 Aug;184 Suppl 1:S58-76
references.pmid: 25061678
references.citation: Parasitology. 2001 May;122(Pt 5):571-82
references.pmid: 11393831
references.citation: J Parasitol. 1985 Feb;71(1):62-9
references.pmid: 3981350
references.citation: J Immunol. 1986 Oct 15;137(8):2555-60
references.pmid: 3531336
references.citation: J Evol Biol. 2011 Aug;24(8):1664-76
references.pmid: 21658142
references.citation: Parasitology. 1992 Dec;105 ( Pt 3):481-92
references.pmid: 1461687
references.citation: Proc Biol Sci. 2009 Oct 7;276(1672):3477-85
references.pmid: 19586947
references.citation: J Anim Ecol. 2012 Jan;81(1):139-49
references.pmid: 21883202
references.citation: Am Nat. 2014 Dec;184(6):764-76
references.pmid: 25438176
references.citation: Parasite Immunol. 2005 Mar;27(3):79-88
references.pmid: 15882234
references.citation: Proc Biol Sci. 2014 Feb 05;281(1779):20132931
references.pmid: 24500168