title: EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge.
abstract: BACKGROUND
  Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection.
  
  
  RESULTS
  EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1-VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge.
  
  
  CONCLUSIONS
  Co-expression of four EV71 structural proteins VP1-VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, People's Republic of China. wxw_wen@163.com.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, People's Republic of China. xiaoxq@bjut.edu.cn.
authors.forename: Xiangqian
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, People's Republic of China. zmxb129@163.com.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, People's Republic of China. liuwei1@bjut.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China. panglin306@sina.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Pang
authors.affiliation: Research Center for Life Science, Beihua University, Jilin, People's Republic of China. sunxinbh@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Virology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Science, Beijing, People's Republic of China. shancen@hotmail.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, 2075 Bayview Avenue, Toronto, M4N 3M5, Canada. byang@sri.utoronto.ca.
authors.forename: Burton B
authors.initials: BB
authors.lastname: Yang
authors.affiliation: Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China. hyming@medmail.com.cn.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, People's Republic of China. shengwang@bjut.edu.cn.
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, People's Republic of China. zengyicdc@sina.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D057134:Antibodies, Neutralizing / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006232:Hand, Foot and Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D056724:Immunity, Humoral / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010957:Plasmids / Q000737:chemistry / Q000276:immunology; D020033:Protein Isoforms / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014611:Vaccination; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; C442459:VP2 protein, Foot-and-mouth disease virus; C442460:VP3 protein, Foot-and-mouth disease virus; D058425:Vaccines, Virus-Like Particle; D014765:Viral Vaccines
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