title: Analysis on correlation between SP and NK-1R and intranasal mucosal contact point headache.
abstract: CONCLUSIONS
  Distribution of SP and NK-1R, especially NK-1R, in nasal mucosal tissue at contact point was higher compared with non-contact point. It was suggested that SP and NK-1R were associated with mucosal contact point headache.
  
  
  OBJECTIVE
  To observe difference of substance P (SP) and NK-1 receptor (NK-1R) expression in tissues at contact point and non-contact point among the patients with intranasal mucosal contact point headache and speculate the role of SP and NK-1R in mucosal contact point headache.
  
  
  METHODS
  SP and NK-1R in tissues of contact point and non-contact point among 40 patients with intranasal mucosal contact point headache were stained histologically by immunohistochemistry, and the mRNA level was detected by RT-PCR.
  
  
  RESULTS
  SP was located in cytoplasm of acini epithelial cells, distributed in nasal mucosa tissues at both contact point and non-contact point. However, stain intensity was significantly increased at contact point (Z = -2.554, p < 0.05). NK-1R was located in the cytoplasm of acinar epithelial cells, inflammatory cells, and nerve fibers of contact point; only in cytoplasm of acinar epithelial cells and nerve fibers of non-contact point. Tinctorial rate of NK-1R increased significantly at contact point (χ(2) = 40.438, p < 0.01). mRNA level of SP and NK-1R was up-regulated in nasal mucosa at contact point compared with non-contact point.
authors.affiliation: a Department of Otolaryngology Head & Neck Surgery , Peking University Ninth School of Clinical Medicine, Beijing Shijitan Hospital , Beijng , PR China ;
authors.forename: Guomin
authors.initials: G
authors.lastname: Zhao
authors.affiliation: b Department of Otolaryngology Head & Neck Surgery , Beijing Shijitan Hospital, Capital Medical University , Beijing , PR China ;
authors.forename: Jinshu
authors.initials: J
authors.lastname: Yin
authors.affiliation: b Department of Otolaryngology Head & Neck Surgery , Beijing Shijitan Hospital, Capital Medical University , Beijing , PR China ;
authors.forename: Hong
authors.initials: H
authors.lastname: Peng
authors.affiliation: c Central Laboratory , Beijing Shijitan Hospital, Capital Medical University , Beijng , PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006261:Headache / Q000209:etiology* / Q000378:metabolism / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D057605:Natural Orifice Endoscopic Surgery; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018040:Receptors, Neurokinin-1; D013373:Substance P
keywords: Headache; NK-1 receptor; contact point; substance P