title: Does hydration status affect MRI measures of brain volume or water content?
abstract: To determine whether differences in hydration state, which could arise from routine clinical procedures such as overnight fasting, affect brain total water content (TWC) and brain volume measured with magnetic resonance imaging (MRI).
  
  
  
  Twenty healthy volunteers were scanned with a 3T MR scanner four times: day 1, baseline scan; day 2, hydrated scan after consuming 3L of water over 12 hours; day 3, dehydrated scan after overnight fasting of 9 hours, followed by another scan 1 hour later for reproducibility. The following MRI data were collected: T2 relaxation (for TWC measurement), inversion recovery (for T1 measurement), and 3D T1 -weighted (for brain volumes). Body weight and urine specific gravity were also measured. TWC was calculated by fitting the T2 relaxation data with a nonnegative least-squares algorithm, with corrections for T1 relaxation and image signal inhomogeneity and normalization to ventricular cerebrospinal fluid. Brain volume changes were measured using SIENA. TWC means were calculated within 14 tissue regions.
  
  
  
  Despite indications of dehydration as demonstrated by increases in urine specific gravity (P = 0.03) and decreases in body weight (P = 0.001) between hydrated and dehydrated scans, there was no measurable change in TWC (within any brain region) or brain volume between hydration states.
  
  
  
  We demonstrate that within a range of physiologic conditions commonly encountered in routine clinical scans (no pretreatment with hydration, well hydrated before MRI, and overnight fasting), brain TWC and brain volumes are not substantially affected in a healthy control cohort. J. Magn. Reson. Imaging 2016;44:296-304.
authors.affiliation: Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Meyers
authors.affiliation: MS/MRI Research Group, University of British Columbia, Vancouver, BC, Canada.|Radiology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Roger
authors.initials: R
authors.lastname: Tam
authors.affiliation: Radiology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Jimmy S
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Shannon H
authors.initials: SH
authors.lastname: Kolind
authors.affiliation: Radiology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Irene M
authors.initials: IM
authors.lastname: Vavasour
authors.affiliation: Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Emilie
authors.initials: E
authors.lastname: Mackie
authors.affiliation: Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Yinshan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Radiology, University of British Columbia, Vancouver, BC, Canada.|Pathology & Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.|International Collaboration on Repair Discoveries, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Laule
authors.affiliation: Philips Healthcare, Hamburg, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Mädler
authors.affiliation: MS/MRI Research Group, University of British Columbia, Vancouver, BC, Canada.|Radiology, University of British Columbia, Vancouver, BC, Canada.|Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: David K B
authors.initials: DK
authors.lastname: Li
authors.affiliation: Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada.|Radiology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Alex L
authors.initials: AL
authors.lastname: MacKay
authors.affiliation: Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Traboulsee
mesh_terms: D000328:Adult; D001834:Body Water / Q000000981:diagnostic imaging*; D001921:Brain / Q000033:anatomy & histology / Q000000981:diagnostic imaging* / Q000502:physiology*; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D004326:Drinking / Q000502:physiology*; D060766:Drinking Water; D005215:Fasting / Q000502:physiology*; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008875:Middle Aged; D009929:Organ Size / Q000502:physiology; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014868:Water Deprivation / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D060766:Drinking Water
keywords: T2 relaxation; brain volume; hydration; magnetic resonance; proton density; water content