title: Partially reduced graphene oxide as highly efficient DNA nanoprobe.
abstract: This work investigates the effect of reduction degree on graphene oxide (GO)-DNA interaction and the fluorescence quenching mechanism. Partial reduced graphene oxide (pRGO), which maintains well water-dispersibility, is synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room temperature. The fluorescence quenching enhances with the restoration degree of sp(2) carbon bonds and follows the static quenching mechanism. The binding constant values imply that pRGO has much stronger affinity with ssDNA than GO. Utilizing this highly efficient nanoprobe, a universal sensing strategy is proposed for homogeneous detection of DNA. Compared with the reported GO-based DNA, this present strategy has obvious advantages such as requirement of low nanoprobe dosage, significantly reduced background, fast fluorescence quenching, and improved sensitivity. Even without any amplification process, the limit of detection can reach as low as 50 pM.
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Hao-Hua
authors.initials: HH
authors.lastname: Deng
authors.affiliation: Department of Laboratory Medicine, Second Hospital of Fuzhou, Fuzhou 350007, China.
authors.forename: Yin-Huan
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Xiao-Qiong
authors.initials: XQ
authors.lastname: Shi
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Peng
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China. Electronic address: 13600885383@139.com.
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Hong
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China. Electronic address: chenandhu@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D002244:Carbon; D006108:Graphite; D004247:DNA
keywords: DNA; Fluorescence; Partially reduced graphene oxide; Quenching; Sensor