title: In vitro antibacterial activity of selected medicinal plants traditionally used in Vietnam against human pathogenic bacteria.
abstract: BACKGROUND
  Medicinal plants are widely used for the treatment of different infectious diseases. Infectious diseases caused by bacteria have a large impact on public health. This study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in Vietnam against the bacterial strains associated with infectious diseases.
  
  
  METHODS
  Methanol extracts of twelve Vietnamese medicinal plants were tested for their antibacterial activity against five bacterial species including Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using the broth microdilution method.
  
  
  RESULTS
  All the plant extracts showed antibacterial activity, especially against Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus). Baeckea frutescens extract revealed a potent activity against the Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 62.5 μg/ml. High activity against all the three Gram-positive bacteria was also observed for the extracts of Cratoxylum formosum ssp. pruniflorum, Pogostemon cablin, and Pedilanthus tithymaloides with MICs of 125, 125 and 250 μg/ml and MBCs of 125-250, 125-250 and 250-500 μg/ml, respectively. The extracts of C. formosum ssp. pruniflorum and P. tithymaloides showed a broad-spectrum antibacterial activity against all the bacteria tested with the MICs of 125-2,000 μg/ml.
  
  
  CONCLUSIONS
  This study indicates clear evidence supporting the traditional use of the plants in treating infectious diseases related to bacteria. In particular, these plant species showed moderate to high antibacterial activity against the Gram-positive bacteria tested.
authors.affiliation: Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, 217 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-333, Republic of Korea.|Division of Bio and Drug Discovery, Korea Research Institute of Chemical Technology, 141 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Thuy Thu
authors.initials: TT
authors.lastname: Vu
authors.affiliation: Division of Green Chemistry and Engineering, Korea Research Institute of Chemical Technology, 141 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Hyungrok
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Pharmaceutical Chemistry and Pesticides Technology, School of Chemical Engineering, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hai Ba Trung, Hanoi, Vietnam.
authors.forename: Vu Khac
authors.initials: VK
authors.lastname: Tran
authors.affiliation: Research and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry (VIIC), No. 2 Pham Ngu Lao, Hoan Kiem, Hanoi, Vietnam.
authors.forename: Quang
authors.initials: Q
authors.lastname: Le Dang
authors.affiliation: Research and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry (VIIC), No. 2 Pham Ngu Lao, Hoan Kiem, Hanoi, Vietnam.
authors.forename: Hoa Thi
authors.initials: HT
authors.lastname: Nguyen
authors.affiliation: Division of Bio and Drug Discovery, Korea Research Institute of Chemical Technology, 141 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Hun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-Ro, Buk-Gu, Gwangju, 500-757, Republic of Korea.
authors.forename: In Seon
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, 217 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-333, Republic of Korea. kjchoi@krict.re.kr.|Division of Bio and Drug Discovery, Korea Research Institute of Chemical Technology, 141 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-600, Republic of Korea. kjchoi@krict.re.kr.
authors.forename: Gyung Ja
authors.initials: GJ
authors.lastname: Choi
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-Ro, Buk-Gu, Gwangju, 500-757, Republic of Korea. kjinc@jnu.ac.kr.
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D014744:Vietnam
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts
references.citation: J Ethnopharmacol. 2012 Sep 28;143(2):631-8
references.pmid: 22867638
references.citation: Fitoterapia. 2006 Jan;77(1):43-6
references.pmid: 16253438
references.citation: BMC Complement Altern Med. 2013 Nov 25;13:329
references.pmid: 24267469
references.citation: Fitoterapia. 2004 Sep;75(6):596-8
references.pmid: 15351117
references.citation: BMC Complement Altern Med. 2014 Jul 14;14:241
references.pmid: 25023038
references.citation: Nat Prod Res. 2014;28(23):2169-72
references.pmid: 24934340
references.citation: J Ethnopharmacol. 2014 Aug 8;155(1):123-31
references.pmid: 24786571
references.citation: Int J Appl Basic Med Res. 2013 Jul;3(2):111-6
references.pmid: 24083146
references.citation: J Ethnopharmacol. 1996 Apr;51(1-3):29-38
references.pmid: 9213624
references.citation: Iran J Pharm Res. 2013 Summer;12(3):307-16
references.pmid: 24250637
references.citation: J Ethnopharmacol. 2010 Oct 28;132(1):101-8
references.pmid: 20678568
references.citation: Molecules. 2013 May 10;18(5):5434-54
references.pmid: 23666009
references.citation: J Ethnopharmacol. 2012 Jun 26;142(1):265-73
references.pmid: 22583961
references.citation: Ann Clin Microbiol Antimicrob. 2014 Oct 30;13:48
references.pmid: 25359605
references.citation: Phytother Res. 2012 Feb;26(2):186-90
references.pmid: 21604309
references.citation: BMC Complement Altern Med. 2014 Apr 04;14:122
references.pmid: 24708514
references.citation: Clin Microbiol Rev. 1999 Oct;12(4):564-82
references.pmid: 10515903
references.citation: J Ethnopharmacol. 2012 Aug 1;142(3):714-22
references.pmid: 22683906
references.citation: Plant Dis. 2013 Dec;97(12):1593-1598
references.pmid: 30716836
references.citation: J Ethnopharmacol. 2014 Aug 8;155(1):852-5
references.pmid: 24945400
references.citation: J Nat Prod. 2008 Aug;71(8):1427-30
references.pmid: 18681481
references.citation: Eur J Microbiol Immunol (Bp). 2013 Dec;3(4):272-4
references.pmid: 24294497