title: Decreased Expression of a Gene Caused by a T-DNA Insertion in an Adjacent Gene in Arabidopsis.
abstract: ALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a T-DNA insertion in one gene can have effects on the expression of neighboring genes.
authors.affiliation: Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Tamura
authors.affiliation: Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Kawabayashi
authors.affiliation: Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shikanai
authors.affiliation: Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Hara-Nishimura
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D060365:Chloroplast Proteins / Q000378:metabolism; D002736:Chloroplasts / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D016254:Mutagenesis, Insertional / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D028861:Nuclear Pore Complex Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C000609086:ALADIN protein, Arabidopsis; D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D004269:DNA, Bacterial; D028861:Nuclear Pore Complex Proteins; C000609087:PSRP5 protein, Arabidopsis; D012269:Ribosomal Proteins; C036483:T-DNA
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