title: Comparison of antioxidant and antiproliferative activity between Kunlun Chrysanthemum flowers polysaccharides (KCCP) and fraction PII separated by column chromatography.
abstract: The aim of the present study was to compare the antioxidant and antiproliferative effects on cancer cells between Kunlun Chrysanthemum flowers polysaccharides (KCCP) and its fraction PII that were separated by Biologic low pressure (LP) chromatography system followed by DEAE cellulose column chromatography. Results of in vitro experiments showed that the reducing power and the scavenging capacity of KCCP towards hydroxyl radicals (OH) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals increased in a concentration dependent manner and were stronger than that of fraction PII. Results of the antiproliferative effect of KCCP and fraction PII on cervical cancer HeLa cells, esophagus cancer Eca109 cells, and mouse ascites hepatomas H22 cells indicated that both KCCP and its fraction PII possessed inhibitory activity on all the tested cancer cells at a dose- and time-dependent manner, with KCCP showing higher inhibitory activity than that of fraction PII. The present study demonstrates that KCCP and its fraction PII have antioxidant properties that may help fight cancers.
authors.affiliation: College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu 214122, China. Electronic address: jingsiqun@163.com.
authors.forename: Siqun
authors.initials: S
authors.lastname: Jing
authors.affiliation: College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chai
authors.affiliation: College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China.
authors.forename: Gai
authors.initials: G
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu 214122, China. Electronic address: xmzhang@jiangnan.edu.cn.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu 214122, China. Electronic address: daihplc@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Pharmaceutical Sciences, UNT System College of Pharmacy, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA. Electronic address: liang-jun.yan@unthsc.edu.
authors.forename: Liang-Jun
authors.initials: LJ
authors.lastname: Yan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002845:Chromatography; D031202:Chrysanthemum / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D006131:Growth Inhibitors; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Analytical; Antioxidant; Antiproliferative effect; Fraction PII; KCCP