title: Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice.
abstract: Attenuated Salmonella typhimurium (S. typhimurium) was selected as a transgenic vehicle for the development of live mucosal vaccines against transmissible gastroenteritis virus (TGEV) based on the M gene. An approximate 1.0 kb DNA fragment, encoding for glycoprotein M, was amplified by RT-PCR and cloned into eukaryotic expression vector pVAX1. The recombinant plasmid pVAX-M was transformed by electroporation into attenuated S. typhimurium SL7207, and the expression and translation of the pVAX-M delivered by recombinant S. typhimurium SL7207 (pVAX-M) was detected both in vitro and in vivo. BALB/c mice were inoculated orally with SL7207 (pVAX-M) at different dosages to evaluate safety of the vaccines. The bacterium was safe to mice at a dosage of 2 × 10(9) CFU, almost eliminated from the spleen and liver at week 4 post-immunization and eventually cleared at week 6. Mice immunized with 1 × 10(9) CFU of SL7207 (pVAX-M) elicited specific anti-TGEV local mucosal and humoral responses including levels of IgA, IgG, IL-4, and IFN-γ as measured by indirect ELISA assay. Moreover, the control groups (pVAX group, PBS group) maintained at a normal level during week 4-8 post-immunization. The results indicated that attenuated S. typhimurium could be used as a delivery vector for oral immunization of TGEV M gene vaccine.
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qing
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.|Sichuan Key Laboratory of Conservation Biology for Endangered Wild Life, Chengdu Research Base of Giant Panda Breeding, Chengdu, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Liu
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. rsghb110@126.com.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Yudi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Qigui
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D000086862:Coronavirus M Proteins; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D005761:Gastroenteritis, Transmissible, of Swine / Q000517:prevention & control; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D018928:Immunity, Mucosal; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D051379:Mice; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology*; D013552:Swine; D014169:Transformation, Bacterial; D005760:Transmissible gastroenteritis virus / Q000235:genetics / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics* / Q000276:immunology*; D019444:Vaccines, DNA; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D000086862:Coronavirus M Proteins; D016207:Cytokines; D007070:Immunoglobulin A; C000706238:M protein, Transmissible gastroenteritis virus; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014763:Viral Matrix Proteins; D014765:Viral Vaccines
keywords: Attenuated Salmonella typhimurium; M gene; Transmissible gastroenteritis virus
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