title: Effects of multiple inhibitory components on anaerobic treatment processes in municipal solid waste incineration leachate.
abstract: This study served to investigate the comparative and combined effects of calcium, ammonia nitrogen, and aquatic humic substances (AHS) on specific methanogenic activity (SMA) in municipal solid waste leachate at mesophilic conditions. Using orthogonal experiments, anaerobic granular sludge was cultured with different concentrations combinations of the three added components for 13 days. The combination of 6000 mg/L calcium, 400 mg/L ammonia nitrogen, and 4000 mg/L AHS was the most inhibitory combination on the SMA of granular sludge, with a calculated 4.49 mL (standard temperature and atmospheric pressure) (STP) CH4/(gVSS·d) of SMA. The SMA with the addition of the inhibitory components was much lower than the control group's (1000 mg/L calcium, 200 mg/L ammonia nitrogen and 2000 mg/L AHS) with a calculated 12.97 mL (STP) CH4/(gVSS·d) of SMA. Calcium was the major inhibitor among the three components followed by AHS. High concentrations of calcium significantly inhibited the utilization of propionate and butyrate in the substrate and further affected the methanogenic process.
authors.affiliation: Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation,College of Environmental Science & Engineering, Beijing Forestry University, 35Tsinghua East Road, Beijing, 100083, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation,College of Environmental Science & Engineering, Beijing Forestry University, 35Tsinghua East Road, Beijing, 100083, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dang
authors.affiliation: Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation,College of Environmental Science & Engineering, Beijing Forestry University, 35Tsinghua East Road, Beijing, 100083, China.
authors.forename: Zhangheng
authors.initials: Z
authors.lastname: Lan
authors.affiliation: Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation,College of Environmental Science & Engineering, Beijing Forestry University, 35Tsinghua East Road, Beijing, 100083, China. sdzlab@126.com.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Sun
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002087:Butyrates / Q000737:chemistry; D002118:Calcium / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D005232:Fatty Acids, Volatile / Q000737:chemistry; D006812:Humic Substances / Q000032:analysis* / Q000382:microbiology; D017745:Incineration; D008697:Methane / Q000737:chemistry; D011422:Propionates / Q000737:chemistry; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D062611:Solid Waste / Q000032:analysis
chemical_list: D002087:Butyrates; D003470:Culture Media; D005232:Fatty Acids, Volatile; D006812:Humic Substances; D011422:Propionates; D012722:Sewage; D062611:Solid Waste; D000641:Ammonia; D008697:Methane; D002118:Calcium
keywords: Anaerobic granular sludge; Biological inhibition; Incineration leachate; Influential compositions; Methanogenic process