title: Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies.
abstract: Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to <10 %. Detergent decellularization completely removed (<5 %) all these activities. These results demonstrate high intensity of extracellular nucleotide metabolism on valve surface and indicate that various valve decellularization techniques differently affect ecto-enzyme activities that could be important in the development of improved valve prostheses.
authors.affiliation: Department of Biochemistry, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kutryb-Zajac
authors.affiliation: Heart Science Centre, Imperial College London, London, UK.
authors.forename: Ada H Y
authors.initials: AH
authors.lastname: Yuen
authors.affiliation: University of Arizona, Tucson, AZ, USA.
authors.forename: Zain
authors.initials: Z
authors.lastname: Khalpey
authors.affiliation: Department of Biochemistry, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Zukowska
authors.affiliation: Department of Biochemistry, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Slominska
authors.affiliation: Heart Science Centre, Imperial College London, London, UK.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Taylor
authors.affiliation: CryoLife, Inc, Kennesaw, GA, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Goldstein
authors.affiliation: CryoLife, Inc, Kennesaw, GA, USA.
authors.forename: Albert E
authors.initials: AE
authors.lastname: Heacox
authors.affiliation: University Milano-Bicocca, Milan, Italy.
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Lavitrano
authors.affiliation: Heart Science Centre, Imperial College London, London, UK.
authors.forename: Adrian H
authors.initials: AH
authors.lastname: Chester
authors.affiliation: Heart Science Centre, Imperial College London, London, UK.
authors.forename: Magdi H
authors.initials: MH
authors.lastname: Yacoub
authors.affiliation: Department of Biochemistry, Medical University of Gdansk, Gdańsk, Poland. rt.smolenski@gmail.com.
authors.forename: Ryszard T
authors.initials: RT
authors.lastname: Smolenski
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000201:enzymology; D001021:Aortic Valve / Q000166:cytology / Q000201:enzymology* / Q000637:transplantation; D001705:Bioprosthesis; D002851:Chromatography, High Pressure Liquid; D003641:Deamination; D003850:Deoxyribonuclease I / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D006350:Heart Valve Prosthesis; D064593:Heterografts; D006868:Hydrolysis; D007038:Hypotonic Solutions; D007700:Kinetics; D009708:Nucleotidases / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013552:Swine; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D003902:Detergents; D007038:Hypotonic Solutions; D012967:Sodium Dodecyl Sulfate; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic; D009708:Nucleotidases; D000241:Adenosine
keywords: (Max 10) heart valves; ATP; Adenosine; Nucleotide metabolism; Valve prostheses; Xenografts
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