title: The nuclear protein GmbZIP110 has transcription activation activity and plays important roles in the response to salinity stress in soybean.
abstract: Plant basic-leucine zipper (bZIP) transcription factors play important roles in many biological processes and are involved in the regulation of salt stress tolerance. Previously, our lab generated digital gene expression profiling (DGEP) data to identify differentially expressed genes in a salt-tolerant genotype of Glycine soja (STGoGS) and a salt-sensitive genotype of Glycine max (SSGoGM). This DGEP data revealed that the expression (log2 ratio) of GmbZIP110 was up-regulated 2.76-fold and 3.38-fold in SSGoGM and STGoGS, respectively. In the present study, the salt inducible gene GmbZIP110 was cloned and characterized through phylogenetic analysis, subcellular localization and in silico transcript abundance analysis in different tissues. The functional role of this gene in salt tolerance was studied through transactivation analysis, DNA binding ability, expression in soybean composite seedlings and transgenic Arabidopsis, and the effect of GmbZIP110 on the expression of stress-related genes in transgenic Arabidopsis was investigated. We found that GmbZIP110 could bind to the ACGT motif, impact the expression of many stress-related genes and the accumulation of proline, Na(+) and K(+), and enhanced the salt tolerance of composite seedlings and transgenic Arabidopsis. Integrating all these results, we propose that GmbZIP110 plays a critical role in the response to salinity stress in soybean and has high potential usefulness in crop improvement.
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Zhaolong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Punjab, Pakistan.
authors.forename: Zulfiqar
authors.initials: Z
authors.lastname: Ali
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: He
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Yi
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Shao
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Ma
authors.affiliation: Provincial Key Laboratory of Agrobiology, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D054712:Salinity; D013025:Glycine max / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013312:Stress, Physiological; D015533:Transcriptional Activation
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D010940:Plant Proteins
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