title: Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size.
abstract: Coffee is one of the most important crops for developing countries. Coffee classification for trading is related to several factors, including grain size. Larger grains have higher market value then smaller ones. Coffee grain size is determined by the development of the perisperm, a transient tissue with a highly active metabolism, which is replaced by the endosperm during seed development. In this study, a proteomics approach was used to identify differentially accumulated proteins during perisperm development in two genotypes with regular (IPR59) and large grain sizes (IPR59-Graudo) in three developmental stages. Twenty-four spots were identified by MALDI-TOF/TOF-MS, corresponding to 15 proteins. We grouped them into categories as follows: storage (11S), methionine metabolism, cell division and elongation, metabolic processes (mainly redox), and energy. Our data enabled us to show that perisperm metabolism in IPR59 occurs at a higher rate than in IPR59-Graudo, which is supported by the accumulation of energy and detoxification-related proteins. We hypothesized that grain and fruit size divergences between the two coffee genotypes may be due to the comparatively earlier triggering of seed development processes in IPR59. We also demonstrated for the first time that the 11S protein is accumulated in the coffee perisperm.
authors.affiliation: Biotechnology Laboratory, Instituto Agronomico do Parana , Londrina, Parana 86047-902, Brazil.|Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina , P.O. Box 6001, Londrina, Parana 86051-990, Brazil.
authors.forename: Leonardo Cardoso
authors.initials: LC
authors.lastname: Alves
authors.affiliation: Biotechnology Laboratory, Instituto Agronomico do Parana , Londrina, Parana 86047-902, Brazil.
authors.forename: Diogo Maciel De
authors.initials: DM
authors.lastname: Magalhães
authors.affiliation: Max Feffer Plant Genetics Laboratory, ESALQ, Universidade de Sao Paulo , Piracicaba, Sao Paulo, Brazil.
authors.forename: Mônica Teresa Veneziano
authors.initials: MT
authors.lastname: Labate
authors.affiliation: Max Feffer Plant Genetics Laboratory, ESALQ, Universidade de Sao Paulo , Piracicaba, Sao Paulo, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Guidetti-Gonzalez
authors.affiliation: Max Feffer Plant Genetics Laboratory, ESALQ, Universidade de Sao Paulo , Piracicaba, Sao Paulo, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Labate
authors.affiliation: Biotechnology Laboratory, Instituto Agronomico do Parana , Londrina, Parana 86047-902, Brazil.
authors.forename: Douglas Silva
authors.initials: DS
authors.lastname: Domingues
authors.affiliation: Biotechnology Laboratory, Instituto Agronomico do Parana , Londrina, Parana 86047-902, Brazil.
authors.forename: Tumoru
authors.initials: T
authors.lastname: Sera
authors.affiliation: Biotechnology Laboratory, Instituto Agronomico do Parana , Londrina, Parana 86047-902, Brazil.
authors.forename: Luiz Gonzaga Esteves
authors.initials: LG
authors.lastname: Vieira
authors.affiliation: Biotechnology Laboratory, Instituto Agronomico do Parana , Londrina, Parana 86047-902, Brazil.|EMBRAPA Café , Brasilia, DF, Brazil.
authors.forename: Luiz Filipe Protasio
authors.initials: LF
authors.lastname: Pereira
mesh_terms: D040503:Coffea / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D003069:Coffee / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003069:Coffee; D010940:Plant Proteins
keywords: grain size; perisperm metabolism; proteomics; quality; seed development