title: Efficacy of Human Adipose Tissue-Derived Stem Cells on Neonatal Bilirubin Encephalopathy in Rats.
abstract: Kernicterus is a neurological syndrome associated with indirect bilirubin accumulation and damages to the basal ganglia, cerebellum and brain stem nuclei particularly the cochlear nucleus. To mimic haemolysis in a rat model such that it was similar to what is observed in a preterm human, we injected phenylhydrazine in 7-day-old rats to induce haemolysis and then infused sulfisoxazole into the same rats at day 9 to block bilirubin binding sites in the albumin. We have investigated the effectiveness of human adiposity-derived stem cells as a therapeutic paradigm for perinatal neuronal repair in a kernicterus animal model. The level of total bilirubin, indirect bilirubin, brain bilirubin and brain iron was significantly increased in the modelling group. There was a significant decreased in all severity levels of the auditory brainstem response test in the two modelling group. Akinesia, bradykinesia and slip were significantly declined in the experience group. Apoptosis in basal ganglia and cerebellum were significantly decreased in the stem cell-treated group in comparison to the vehicle group. All severity levels of the auditory brainstem response tests were significantly decreased in 2-month-old rats. Transplantation results in the substantial alleviation of walking impairment, apoptosis and auditory dysfunction. This study provides important information for the development of therapeutic strategies using human adiposity-derived stem cells in prenatal brain damage to reduce potential sensori motor deficit.
authors.affiliation: Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Amini
authors.affiliation: Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Iranian National Center for Addiction Studies (INCAS), Iranian Institute for Reduction of High-Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Vousooghi
authors.affiliation: Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahmoudreza
authors.initials: M
authors.lastname: Hadjighassem
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Bakhtiyari
authors.affiliation: Department of Cellular and Molecular Nutrition, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Mousavi
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Hosein
authors.initials: H
authors.lastname: Safakheil
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Jafari
authors.affiliation: Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Sarveazad
authors.affiliation: Department of Anatomy, Alborz University of Medical Sciences, Karaj, Iran.
authors.forename: Abazar
authors.initials: A
authors.lastname: Yari
authors.affiliation: Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Ramezani
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Faghihi
authors.affiliation: Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. mt.joghataei@yahoo.com.|Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. mt.joghataei@yahoo.com.|Neuroscience Department, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. mt.joghataei@yahoo.com.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Joghataei
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000831:Animals, Newborn; D000890:Anti-Infective Agents / Q000633:toxicity; D015703:Antigens, CD / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006801:Humans; D007501:Iron / Q000378:metabolism; D007647:Kernicterus / Q000139:chemically induced / Q000150:complications / Q000601:surgery*; D008297:Male; D016877:Oxidants / Q000633:toxicity; D010659:Phenylhydrazines / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D055139:Sensory Gating / Q000187:drug effects; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000502:physiology*; D013444:Sulfisoxazole / Q000633:toxicity
chemical_list: D000890:Anti-Infective Agents; D015703:Antigens, CD; D016877:Oxidants; D010659:Phenylhydrazines; C030299:phenylhydrazine; D013444:Sulfisoxazole; D007501:Iron
keywords: Kernicterus; Phenyl hydrazine; Stem cell; Sulfisoxazole
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