title: Proteomic Analysis of Rhizoctonia solani Identifies Infection-specific, Redox Associated Proteins and Insight into Adaptation to Different Plant Hosts.
abstract: Rhizoctonia solaniis an important root infecting pathogen of a range of food staples worldwide including wheat, rice, maize, soybean, potato and others. Conventional resistance breeding strategies are hindered by the absence of tractable genetic resistance in any crop host. Understanding the biology and pathogenicity mechanisms of this fungus is important for addressing these disease issues, however, little is known about howR. solanicauses disease. This study capitalizes on recent genomic studies by applying mass spectrometry based proteomics to identify soluble, membrane-bound and culture filtrate proteins produced under wheat infection and vegetative growth conditions. Many of the proteins found in the culture filtrate had predicted functions relating to modification of the plant cell wall, a major activity required for pathogenesis on the plant host, including a number found only under infection conditions. Other infection related proteins included a high proportion of proteins with redox associated functions and many novel proteins without functional classification. The majority of infection only proteins tested were confirmed to show transcript up-regulation during infection including a thaumatin which increased susceptibility toR. solaniwhen expressed inNicotiana benthamiana In addition, analysis of expression during infection of different plant hosts highlighted how the infection strategy of this broad host range pathogen can be adapted to the particular host being encountered. Data are available via ProteomeXchange with identifier PXD002806.
authors.affiliation: From the ‡CSIRO Agriculture, Floreat, Western Australia; §The University of Western Australia Institute of Agriculture, Crawley, Western Australia;
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Anderson
authors.affiliation: From the ‡CSIRO Agriculture, Floreat, Western Australia;
authors.forename: James K
authors.initials: JK
authors.lastname: Hane
authors.affiliation: ¶QIMR Berghofer Medical Research Institute, Herston, QLD, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Stoll
authors.affiliation: From the ‡CSIRO Agriculture, Floreat, Western Australia;
authors.forename: Nicholas
authors.initials: N
authors.lastname: Pain
authors.affiliation: ¶QIMR Berghofer Medical Research Institute, Herston, QLD, Australia.
authors.forename: Marcus L
authors.initials: ML
authors.lastname: Hastie
authors.affiliation: From the ‡CSIRO Agriculture, Floreat, Western Australia;
authors.forename: Parwinder
authors.initials: P
authors.lastname: Kaur
authors.affiliation: ¶QIMR Berghofer Medical Research Institute, Herston, QLD, Australia.
authors.forename: Christine
authors.initials: C
authors.lastname: Hoogland
authors.affiliation: ¶QIMR Berghofer Medical Research Institute, Herston, QLD, Australia.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Gorman
authors.affiliation: From the ‡CSIRO Agriculture, Floreat, Western Australia; §The University of Western Australia Institute of Agriculture, Crawley, Western Australia; Karam.Singh@csiro.au.
authors.forename: Karam B
authors.initials: KB
authors.lastname: Singh
mesh_terms: D000222:Adaptation, Physiological; D005656:Fungal Proteins / Q000378:metabolism; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry / Q000379:methods; D010084:Oxidation-Reduction; D010935:Plant Diseases / Q000382:microbiology; D040901:Proteomics / Q000379:methods*; D012232:Rhizoctonia / Q000378:metabolism / Q000472:pathogenicity*; D014908:Triticum / Q000382:microbiology*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D037521:Virulence Factors
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