title: Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers.
abstract: In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562).
authors.forename: Gerardo
authors.initials: G
authors.lastname: Perozziello
authors.forename: Patrizio
authors.initials: P
authors.lastname: Candeloro
authors.forename: Antonio
authors.initials: A
authors.lastname: De Grazia
authors.forename: Francesco
authors.initials: F
authors.lastname: Esposito
authors.forename: Marco
authors.initials: M
authors.lastname: Allione
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Coluccio
authors.forename: Rossana
authors.initials: R
authors.lastname: Tallerico
authors.forename: Immanuel
authors.initials: I
authors.lastname: Valpapuram
authors.forename: Luca
authors.initials: L
authors.lastname: Tirinato
authors.forename: Gobind
authors.initials: G
authors.lastname: Das
authors.forename: Andrea
authors.initials: A
authors.lastname: Giugni
authors.forename: Bruno
authors.initials: B
authors.lastname: Torre
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Veltri
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kruhne
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Della Valle
authors.forename: Enzo
authors.initials: E
authors.lastname: Di Fabrizio
mesh_terms: D019281:Dimerization; D006801:Humans; D020014:K562 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D055620:Optical Phenomena; D059010:Single-Cell Analysis / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*