title: P15, MDM2, NF-κB, and Bcl-2 expression in primary bone tumor and correlation with tumor formation and metastasis.
abstract: Primary bone tumor is one of the most common malignant tumors in skeletal system. It seriously affected bone movement and development with unclear pathogenesis. In this paper, rabbit VX-2 malignant bone tumor model was applied to explore apoptotic genes P15, MDM2, NF-κB and Bcl-2 correlation with primary bone tumor occurrence and metastasis. 0.3 ml rabbit VX-2 tumor cell suspension (1×10(6)/ml) was injected to the marrow cavity of the right tibia condyle to establish the rabbit malignant bone tumor model, while equal amount of the saline was injected to the left tibia as control. Real-time PCR was applied to determine P15, MDM2, NF-κB and Bcl-2 expression level. Immunohistochemistry was performed to detect the abovementioned genes expression in lung, stomach, kidney and bladder. Compared with control, P15 expression level in the inoculation site surrounding tissues decreased obviously following the inoculate time elongation (P<0.05), while Bcl-2, MDM2 and NF-κB expression significantly increased (P<0.05). Bcl-2 showed significant correlation with MDM2 and NF-κB (P<0.05). At the 2, 4, 6 weeks, Bcl-2, MDM2 and NF-κB in lung, Bcl-2 in kidney, and Bcl-2 and MDM2 in bladder positively expressed (P<0.05), whereas P15 gene exhibited no significant positive expression in these tissues (P>0.05). P15, MDM2, NF-κB, and Bcl-2 genes expression levels can effectively reflect malignant bone tumor growth of rabbit tibia. MDM2, NF-κB and Bcl-2 genes involved in primary bone tumors metastasis directly. It has important clinical significance for early diagnosis and treatment of primary bone tumor.
authors.affiliation: Department of Orthopaedics, The Forth Affiliated Hospital of Harbin Medical University Harbin 150000, Heilongjiang, China.
authors.forename: Guibin
authors.initials: G
authors.lastname: Qian
authors.affiliation: Department of Orthopaedics, The Fifth Hospital of Harbin Harbin 150000, Heilongjiang, China.
authors.forename: Songnan
authors.initials: S
authors.lastname: Hao
authors.affiliation: Department of Orthopaedics, The Forth Affiliated Hospital of Harbin Medical University Harbin 150000, Heilongjiang, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, The First Hospital of Harbin Harbin 150000, Heilongjiang, China.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D014408:Biomarkers, Tumor / Q000032:analysis*; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D064593:Heterografts; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000032:analysis / Q000096:biosynthesis; D009361:Neoplasm Invasiveness / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis / Q000096:biosynthesis; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D051736:Proto-Oncogene Proteins c-mdm2
keywords: Bcl-2; Bone tumor; MDM2; NF-ΚB; P15; apoptosis
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