title: [MOLECULAR MECHANISMS OF ERK1/2 KINASES REGULATION IN THE GLUTAMATE- AND GABA-ERGIC NEURONS DURING SEIZURE EXPRESSION IN KRUSHINSKY-MOLODKINA RATS].
abstract: The aim of the present study was to analyze a role of the ERK1/2 signaling pathway in the regulation of excitation and inhibitory neurons in the hippocampus and the temporal cortex of Krushinsky-Molodkina rats during seizure development finalizing with ataxia. Analysis was done by Western bloting as well as by immunohistochemistry. The results demonstrated significant up-regulation of ERK1/2 activity in the hippocampus in several seconds after sound stimulation. At the same time increased ERK1/2 activity was correlated with enhanced level of SNARE protein SNAP-25 and activation of synapsin I, the proteins which regulate exocytosis machinery. Decreased level of VGLUT2 associated with activation of ERK1/2 and exocytosis proteins supposed activation of glutamate release in the hippocampus, while in the temporal cortex diminished activity of ERK1/2 and synapsin I associated with VGLUT2 up-regulation assumed inhibition of glutamatergic transmission. Our data let us supposed that decreasing of glutamate release in th& temporal cortex could be a trigger for the inhibition of hippocampal glutamatergic system and the beginning of further ataxia stage. Our data demonstrated correlation between expression and activity of exocytosis proteins and ERK1/2 mainly in the glutamategic neurons of the hippocampus and the temporal cortex that let us proposed significant role of ERK1/2 kinases as a positive regulator of glutamate release and as a result initiation of seizure expression.
authors.forename: A A
authors.initials: AA
authors.lastname: Korotkov
authors.forename: M V
authors.initials: MV
authors.lastname: Glazova
authors.forename: L S
authors.initials: LS
authors.lastname: Nikitina
authors.forename: N A
authors.initials: NA
authors.lastname: Dorofeeva
authors.forename: O D
authors.initials: OD
authors.lastname: Kirillova
authors.forename: E V
authors.initials: EV
authors.lastname: Chernigovskaya
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D017849:Animals, Inbred Strains; D001259:Ataxia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020195:Epilepsy, Reflex / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005089:Exocytosis / Q000235:genetics; D059330:GABAergic Neurons / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics* / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D012640:Seizures / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction; D016704:Synapsins / Q000235:genetics / Q000378:metabolism; D050825:Synaptosomal-Associated Protein 25 / Q000235:genetics / Q000378:metabolism; D013702:Temporal Lobe / Q000378:metabolism / Q000473:pathology; D050599:Vesicular Glutamate Transport Protein 2 / Q000235:genetics / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C493475:Slc17a6 protein, rat; C494210:Snap25 protein, rat; D016704:Synapsins; D050825:Synaptosomal-Associated Protein 25; D050599:Vesicular Glutamate Transport Protein 2; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; C535149:Mapk1 protein, rat; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3