title: Temperature and pH dependence of DNA ejection from archaeal lemon-shaped virus His1.
abstract: The archaeal virus His1 isolated from a hypersaline environment infects an extremely halophilic archaeon Haloarcula hispanica. His1 features a lemon-shaped capsid, which is so far found only in archaeal viruses. This unique capsid can withstand high salt concentrations, and can transform into a helical tube, which in turn is resistant to extremely harsh conditions. Hypersaline environments exhibit a wide range of temperatures and pH conditions, which present an extra challenge to their inhabitants. We investigated the influence of pH and temperature on DNA ejection from His1 virus using single-molecule fluorescence experiments. The observed number of ejecting viruses is constant in pH 5 to 9, while the ejection process is suppressed at pH below 5. Similarly, the number of ejections within 15-42 °C shows only a minor increase around 25-37 °C. The maximum velocity of single ejected DNA increases with temperature, in qualitative agreement with the continuum model of dsDNA ejection.
authors.affiliation: Department of Physics, University of Helsinki, Helsinki, Finland. kalle.hanhijarvi@helsinki.fi.
authors.forename: K J
authors.initials: KJ
authors.lastname: Hanhijärvi
authors.affiliation: Department of Biosciences, University of Helsinki, Helsinki, Finland.
authors.forename: G
authors.initials: G
authors.lastname: Ziedaite
authors.affiliation: Department of Physics, University of Helsinki, Helsinki, Finland.
authors.forename: E
authors.initials: E
authors.lastname: Hæggström
authors.affiliation: Institute of Biotechnology and Department of Biosciences, University of Helsinki, Helsinki, Finland.
authors.forename: D H
authors.initials: DH
authors.lastname: Bamford
mesh_terms: D023641:Archaeal Viruses / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002213:Capsid / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D023281:Genomics; D054884:Host-Pathogen Interactions; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D004279:DNA, Viral
keywords: Archaeal virus His1; DNA ejection; DNA mobility; Fluorescence microscopy
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