title: DAZL and CPEB1 regulate mRNA translation synergistically during oocyte maturation.
abstract: Meiotic progression requires exquisitely coordinated translation of maternal messenger (m)RNA that has accumulated during oocyte growth. A major regulator of this program is the cytoplasmic polyadenylation element binding protein 1 (CPEB1). However, the temporal pattern of translation at different meiotic stages indicates the function of additional RNA binding proteins (RBPs). Here, we report that deleted in azoospermia-like (DAZL) cooperates with CPEB1 to regulate maternal mRNA translation. Using a strategy that monitors ribosome loading onto endogenous mRNAs and a prototypic translation target, we show that ribosome loading is induced in a DAZL- and CPEB1-dependent manner, as the oocyte reenters meiosis. Depletion of the two RBPs from oocytes and mutagenesis of the 3' untranslated regions (UTRs) demonstrate that both RBPs interact with the Tex19.1 3' UTR and cooperate in translation activation of this mRNA. We observed a synergism between DAZL and cytoplasmic polyadenylation elements (CPEs) in the translation pattern of maternal mRNAs when using a genome-wide analysis. Mechanistically, the number of DAZL proteins loaded onto the mRNA and the characteristics of the CPE might define the degree of cooperation between the two RBPs in activating translation and meiotic progression.
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Joao P
authors.initials: JP
authors.lastname: Sousa Martins
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
authors.forename: Ashwini
authors.initials: A
authors.lastname: Oke
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Ripla
authors.initials: R
authors.lastname: Arora
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, 20133, Milano, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Franciosi
authors.affiliation: Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale INSERM U964, Centre National de Recherche Scientifique CNRS UMR 1704, Université de Strasbourg, Illkirch 67404, France Centre Hospitalier Universitaire, Strasbourg F-67000, France.
authors.forename: Stephan
authors.initials: S
authors.lastname: Viville
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Diana J
authors.initials: DJ
authors.lastname: Laird
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Fung
authors.affiliation: Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA contim@obgyn.ucsf.edu.
authors.forename: Marco
authors.initials: M
authors.lastname: Conti
mesh_terms: D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008540:Meiosis; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D009866:Oogenesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C490387:Cpeb1 protein, mouse; C508515:DAZL protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors
keywords: CPEB; DAZL; Oocyte maturation; RBPs; Translation control
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