title: Genotyping of single nucleotide polymorphisms related to attention-deficit hyperactivity disorder.
abstract: Pharmacological treatment of several diseases, such as attention-deficit hyperactivity disorder (ADHD), presents marked variability in efficiency and its adverse effects. The genotyping of specific single nucleotide polymorphisms (SNPs) can support the prediction of responses to drugs and the genetic risk of presenting comorbidities associated with ADHD. This study presents two rapid and affordable microarray-based strategies to discriminate three clinically important SNPs in genes ADRA2A, SL6CA2, and OPRM1 (rs1800544, rs5569, and rs1799971, respectively). These approaches are allele-specific oligonucleotide hybridization (ASO) and a combination of allele-specific amplification (ASA) and solid-phase hybridization. Buccal swab and blood samples taken from ADHD patients and controls were analyzed by ASO, ASA, and a gold-reference method. The results indicated that ASA is superior in genotyping capability and analytical performance.
authors.affiliation: Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Tortajada-Genaro
authors.affiliation: Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Mena
authors.affiliation: Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: Regina
authors.initials: R
authors.lastname: Niñoles
authors.affiliation: AB-Biotics S.A., Edifici Eureka, Campus UAB, 08193, Barcelona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Puigmule
authors.affiliation: AB-Biotics S.A., Edifici Eureka, Campus UAB, 08193, Barcelona, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Viladevall
authors.affiliation: Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain. amaquieira@qim.upv.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
mesh_terms: D000483:Alleles; D001289:Attention Deficit Disorder with Hyperactivity / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide
keywords: Attention-deficit hyperactivity disorder; Low-cost microarray technology; Pharmacogenetics; SNP genotyping