title: Morphological and molecular characterization of Isospora manorinae n. sp. in a yellow-throated miner (Manorina flavigula wayensis) (Gould, 1840).
abstract: A new Isospora (Apicomplexa:Eimeriidae) species is described from a single yellow-throated miner bird (Manorina flavigula) (subspecies M. f. wayensis) in Western Australia. Sporulated oocysts (n = 32) of this isolate are spherical to subspherical, 22.8 (20.3-23.8) × 18.3 (17.7-18.7) μm, with a shape index (length/width) of 1.25 (1.2-1.3); and a smooth and bilayered oocyst wall, 1.3 μm thick (outer layer 0.9 μm, inner 0.4 μm). A polar granule is present, but the micropyle and oocyst residuum are absent. The sporocysts are lemon-shaped, 15.5 (14.6-15.8) × 9.5 (9.5-10.2) μm, with a shape index of 1.6. Stieda and substieda bodies are present, the Stieda body being knob-like and the substieda body being subspherical-shaped. A sporocyst residuum is present and composed of numerous granules of different size scattered among the sporozoites, a spheroid or subspheroid refractile body is present in the sporozoite. Morphologically, the oocysts from this isolate are different from those of all known valid Isospora spp. Molecular analysis was conducted at 3 loci; the 18S and 28S ribosomal RNA and the mitochondrial cytochrome oxidase (COI) gene. At the 18S locus, this new isolate exhibited 99.2% similarity to Isospora gryphoni and three other Isospora spp. Further analysis of a subgroup of 300 bp long 18S sequences (8), including Isospora anthochaerae was conducted. This new isolate grouped in a clade with I. anthochaerae and exhibited 99.3% similarity. At the 28S locus, this new isolate grouped with I. anthochaerae with which it shared 99.1% similarity. At the COI locus, this new isolate exhibited 96.8% similarity to Isospora sp. JCI-2015 from a spectacled warbler (Sylvia conspicillata) in Spain. Further analysis from a subgroup of shorter COI sequences (n = 13) was performed and this new isolate exhibited 99.1% similarity to I. anthochaerae. Based on morphological and molecular data, this isolate is a new species of Isospora, which is named Isospora manorinae n. sp. after its host, the yellow-throated miner (Manorina flavigula wayensis).
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia. Electronic address: R.Yang@murdoch.edu.au.
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.affiliation: Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Brice
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, 450002, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Jian
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000469:parasitology*; D003576:Electron Transport Complex IV / Q000235:genetics; D019143:Evolution, Molecular; D005243:Feces / Q000469:parasitology; D007549:Isospora / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D021865:Isosporiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008928:Mitochondria / Q000201:enzymology; D033661:Oocysts / Q000648:ultrastructure; D046109:Passeriformes / Q000469:parasitology*; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D016415:Sequence Alignment; D014904:Western Australia / Q000453:epidemiology
chemical_list: D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D003576:Electron Transport Complex IV
keywords: 18S rRNA; 28S rRNA; COI; Isospora; Morphology; Phylogeny; Yellow-throated miner