title: Assessment of Matrix Metalloproteinases by Gelatin Zymography.
abstract: Matrix metalloproteinases are endopeptidases responsible for remodeling of the extracellular matrix and have been identified as critical contributors to breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression. In this approach, enzymes are resolved electrophoretically on a sodium dodecyl sulfate-polyacrylamide gel copolymerized with the substrate for the MMP of interest. Post electrophoresis, the enzymes are refolded in order for proteolysis of the incorporated substrate to occur. This assay yields valuable information about MMP isoforms or changes in activation and can be used to analyze the role of MMPs in normal versus pathological conditions.
authors.affiliation: Department of Cellular and Molecular Pharmacology, Stony Brook University, 100 Nicolls Road, Stony Brook, NY, 11794, USA. Jillian.Cathcart@StonyBrook.edu.
authors.forename: Jillian
authors.initials: J
authors.lastname: Cathcart
mesh_terms: D053000:Analytic Sample Preparation Methods; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*; D005780:Gelatin; D006801:Humans; D006868:Hydrolysis; D020782:Matrix Metalloproteinases / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D005780:Gelatin; D020782:Matrix Metalloproteinases
keywords: MMPs; Matrix metalloproteinases; Zymogram; Zymography