title: Enhanced poly(L-malic acid) production from pretreated cane molasses by Aureobasidium pullulans in fed-batch fermentation.
abstract: Poly(L-malic acid) (PMA) is a natural polyester with many attractive properties for biomedical application. However, the cost of PMA production is high when glucose is used as a carbon source. To solve this problem, cane molasses as a low-cost feedstock was applied for the production of PMA. Six pretreatment methods were applied to cane molasses before fermentation. Pretreatment with combined tricalcium phosphate, potassium ferrocyanide, and sulfuric acid (TPFSA) removed significant amounts of metal ions from cane molasses. The PMA concentration increased from 5.4 g/L (untreated molasses) to 36.9 g/L (TPFSA-pretreated molasses) after fermentation in shake flasks. A fed-batch fermentation strategy was then developed. In this method, TPFSA-pretreated cane molasses solution was continuously fed into the fermentor to maintain the total sugar concentration at 20 g/L. This technique generated approximately 95.4 g/L PMA with a productivity of 0.57 g/L/hr. The present study indicated that fed-batch fermentation using pretreated cane molasses is a feasible technique for producing high amounts of PMA.
authors.affiliation: a Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering , Huaiyin Normal University , Huai'an , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.affiliation: a Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering , Huaiyin Normal University , Huai'an , China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Xu
authors.affiliation: a Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering , Huaiyin Normal University , Huai'an , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hu
authors.affiliation: a Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering , Huaiyin Normal University , Huai'an , China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D008293:Malates / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D031786:Saccharum / Q000378:metabolism*
chemical_list: D008293:Malates; D011108:Polymers; C121060:poly(malic acid)
keywords: Aureobasidium pullulans; cane molasses; poly(L-malic acid); pretreatment methods