title: Architectural Analysis of Picrosirius Red Stained Collagen in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma using Polarization Microscopy.
abstract: BACKGROUND
  Collagen degradation is important both for carcinogenesis and in its progression. Research regarding the co-relation of collagen with Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) is less explored.
  
  
  OBJECTIVE
  To elucidate the nature of collagen in Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) using Picrosirius Red Stain (PSR) under polarizing microscopy.
  
  
  METHODS
  The study consisted of a total 40 samples which were divided into three groups. Group I included buccal mucosa as negative and irritation fibroma as positive control, group II consisted of OED and group III consisted of Oral Squamous Cell Carcinoma (OSCC). A histochemical analysis was conducted using PSR-polarization method by two independent observers.
  
  
  RESULTS
  The control group shows predominantly reddish-orange birefringence. In OED with the advancement of grades, the colour changed from yellowish-orange colour to yellow-greenish with progressive increase in greenish hue. As OSCC regresses from well to poorly differentiated, the colour changed from reddish-orange to yellowish orange to greenish-yellow suggesting a transition from mature to immature collagen.
  
  
  CONCLUSIONS
  An observable gradual change in collagen of both OED and OSCC was noted as they were proceeding from benign to critical step. Thus, PSR is a useful tool for studying stromal changes as supporting collagen shows the transition in the form besides the alterations in epithelial cells.
authors.affiliation: Senior Lecturer, Department Oral Pathology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, Haryana, India .
authors.forename: Rashi
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Reader, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research , Faridabad, Haryana, India .
authors.forename: Shweta
authors.initials: S
authors.lastname: Rehani
authors.affiliation: Reader, Department of Oral Pathology, ITS Dental College , Greater Noida, Uttar Pradesh, India .
authors.forename: Monica
authors.initials: M
authors.lastname: Mehendiratta
authors.affiliation: Senior Lecturer, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research , Faridabad, Haryana, India .
authors.forename: Priyanka
authors.initials: P
authors.lastname: Kardam
authors.affiliation: Professor, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, Haryana, India .
authors.forename: Madhumani
authors.initials: M
authors.lastname: Kumra
authors.affiliation: Professor and Head, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research , Faridabad, Haryana, India .
authors.forename: Yulia
authors.initials: Y
authors.lastname: Mathias
authors.affiliation: Senior Lecturer, Department of Oral Pathology and Mirobiology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, India .
authors.forename: Jyoti
authors.initials: J
authors.lastname: Yadav
authors.affiliation: Senior Lecturer, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, Haryana, India .
authors.forename: Khushboo
authors.initials: K
authors.lastname: Sahay
keywords: Birefringence; Greenish yellow; Reddish orange; Yellowish orange
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