title: CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography).
abstract: FINCH holographic fluorescence microscopy creates high resolution super-resolved images with enhanced depth of focus. The simple addition of a real-time Nipkow disk confocal image scanner in a conjugate plane of this incoherent holographic system is shown to reduce the depth of focus, and the combination of both techniques provides a simple way to enhance the axial resolution of FINCH in a combined method called "CINCH". An important feature of the combined system allows for the simultaneous real-time image capture of widefield and holographic images or confocal and confocal holographic images for ready comparison of each method on the exact same field of view. Additional GPU based complex deconvolution processing of the images further enhances resolution.
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University, 9605 Medical Center Drive, Rockville, MD 20850 USA; Microscopy Center, Johns Hopkins University Montgomery County Campus, Rockville, MD 20850 USA.
authors.forename: Nisan
authors.initials: N
authors.lastname: Siegel
authors.affiliation: Department of Physiology and Biophysics University of Arkansas for Medical Sciences, Little Rock, AR 72205 USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Storrie
authors.affiliation: Microvolution, Danville, CA, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Bruce
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University, 9605 Medical Center Drive, Rockville, MD 20850 USA; Microscopy Center, Johns Hopkins University Montgomery County Campus, Rockville, MD 20850 USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Brooker
keywords: FINCH; Fresnel incoherent correlation holography; GPU; Holography; Nipkow disk; confocal microscopy; deconvolution; fluorescence microscopy; super-resolution
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