title: Lotus japonicus plants of the Gifu B-129 ecotype subjected to alkaline stress improve their Fe(2+) bio-availability through inoculation with Pantoea eucalypti M91.
abstract: Inoculation assays with Pantoea eucalypti M91 were performed on Lotus japonicus ecotype Gifu. Under alkaline conditions, this ecotype is characterized by the development of interveinal chlorosis of the apical leaves due to low mobilization of Fe(2+). Inoculation with P. eucalypti M91, a plant growth-promoting bacterial strain capable of producing pyoverdine-like and pyochelin-like siderophores under alkaline growth conditions, alters the root, resulting in a herringbone pattern of root branching. Additional features include improvement in Fe(2+) transport to the shoots, acidification of the hydroponic solution of the plant cultures, and an accompanying increase in the efficiency of the PSII parameters. In addition, there was an increase in the expression of the FRO1 and IRT1 genes, accompanied by a significant increase in FRO activity. Results showed that P. eucalypti M91 has a beneficial effect on the Fe acquisition machinery of Strategy I, as described for non-graminaceous monocots and dicots, suggesting its potential as an inoculant for legume crops cultivated in alkaline soils.
authors.affiliation: Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8,2 CC 164, B7130IWA Chascomús, Argentina. Electronic address: mpcampestre@intech.gov.ar.
authors.forename: María Paula
authors.initials: MP
authors.lastname: Campestre
authors.affiliation: Laboratorio de Microbiología del Suelo, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8,2 CC 164, B7130IWA Chascomús, Argentina. Electronic address: ncastagno@intech.gov.ar.
authors.forename: Luis Nazareno
authors.initials: LN
authors.lastname: Castagno
authors.affiliation: Laboratorio de Microbiología del Suelo, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8,2 CC 164, B7130IWA Chascomús, Argentina. Electronic address: estrella@intech.gov.ar.
authors.forename: María Julia
authors.initials: MJ
authors.lastname: Estrella
authors.affiliation: Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8,2 CC 164, B7130IWA Chascomús, Argentina. Electronic address: ruiz@intech.gov.ar.
authors.forename: Oscar Adolfo
authors.initials: OA
authors.lastname: Ruiz
mesh_terms: D018556:Crops, Agricultural; D060146:Ecotype; D018506:Gene Expression Regulation, Plant; D018527:Hydroponics; D000070116:Lotus / Q000235:genetics / Q000502:physiology*; D020636:Pantoea; D018515:Plant Leaves; D018517:Plant Roots / Q000378:metabolism; D017262:Siderophores / Q000235:genetics / Q000378:metabolism
chemical_list: D017262:Siderophores
keywords: Alkaline soils; Fe deficiency; Lotus japonicus; PGPB; Pantoea sp.