title: Analysis of Invadopodia Formation in Breast Cancer Cells.
abstract: Metastasis is the major cause of breast cancer deaths. To spread from the primary tumor sites to distant tissues, solid tumor cells need to degrade the surrounding extracellular matrix (ECM). The protrusive membrane structures named invadopodia have been shown to play a critical role in the degradation of the ECM and invasion of invasive cancer cells. In this chapter, we describe a detailed protocol to examine invadopodia in human breast cancer cells.
authors.affiliation: Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, 6431 Fannin St, Houston, TX, 77030, USA.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, 6431 Fannin St, Houston, TX, 77030, USA.|Shanghai Institute of Digestive Disease, Shanghai Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200001, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, 6431 Fannin St, Houston, TX, 77030, USA.|Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430022, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cai
authors.affiliation: Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, 6431 Fannin St, Houston, TX, 77030, USA. guangwei.du@uth.tmc.edu.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Du
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D009361:Neoplasm Invasiveness; D000069261:Podosomes / Q000473:pathology*
chemical_list: D005456:Fluorescent Dyes; D005780:Gelatin
keywords: Breast cancer; Extracellular matrix; Invadopodia; Invasion; Metastasis
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