title: Differential Isotope Labeling of 38 Dietary Polyphenols and Their Quantification in Urine by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry.
abstract: A large number of polyphenols are consumed with the diet and may contribute to the prevention of chronic diseases such as cardiovascular diseases, diabetes, cancers, and neurodegenerative diseases. More comprehensive methods are needed to measure exposure to this complex family of bioactive plant compounds in epidemiological studies. We report here a novel method enabling the simultaneous measurement in urine of 38 polyphenols representative of the main classes and subclasses found in the diet. This method is based on differential (12)C-/(13)C-isotope labeling of polyphenols through derivatization with isotopic dansyl chloride reagents and on the analysis of the labeled polyphenols by tandem mass spectrometry. This derivatization approach overcomes the need for costly labeled standards. Different conditions for enzyme hydrolysis of polyphenol glucuronides and sulfate esters, extraction, and dansylation of unconjugated aglycones were tested and optimized. Limits of quantification varied from 0.01 to 1.1 μM depending on polyphenols. Intrabatch coefficients of variation varied between 3.9% and 9.6%. Interbatch variations were lower than 15% for 31 compounds and lower than 29% for 6 additional polyphenols out of the 38 tested. Thirty seven polyphenols were validated and then analyzed in 475, 24 h urine samples from the European Prospective Investigation on Cancer and Nutrition (EPIC) study. Thirty four polyphenols could be detected and successfully estimated and showed large interindividual variations of concentrations (2-3 orders of magnitude depending on the compound), with median concentrations spanning from 0.01 to over 1000 μM for all 34 compounds.
authors.affiliation: Biomarkers Group, Nutrition and Metabolism Section, International Agency for Research on Cancer (IARC) , 150 cours Albert Thomas, Lyon 69372 Cedex 08, France.
authors.forename: David
authors.initials: D
authors.lastname: Achaintre
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université de Lyon 1, ENS-Lyon, 5 rue de la Doua, Villeurbanne 69100, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Buleté
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université de Lyon 1, ENS-Lyon, 5 rue de la Doua, Villeurbanne 69100, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Cren-Olivé
authors.affiliation: Department of Chemistry, University of Alberta , Edmonton, Alberta, T6G 2G2, Canada.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Biomarkers Group, Nutrition and Metabolism Section, International Agency for Research on Cancer (IARC) , 150 cours Albert Thomas, Lyon 69372 Cedex 08, France.
authors.forename: Sabina
authors.initials: S
authors.lastname: Rinaldi
authors.affiliation: Biomarkers Group, Nutrition and Metabolism Section, International Agency for Research on Cancer (IARC) , 150 cours Albert Thomas, Lyon 69372 Cedex 08, France.
authors.forename: Augustin
authors.initials: A
authors.lastname: Scalbert
mesh_terms: D002250:Carbon Radioisotopes; D002853:Chromatography, Liquid; D003619:Dansyl Compounds / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D057230:Limit of Detection; D059808:Polyphenols / Q000737:chemistry / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002250:Carbon Radioisotopes; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D059808:Polyphenols