title: Sequencing ebola and marburg viruses genomes using microarrays.
abstract: Periodic outbreaks of Ebola and Marburg hemorrhagic fevers have occurred in Africa over the past four decades with case fatality rates reaching as high as 90%. The latest Ebola outbreak in West Africa in 2014 raised concerns that these infections can spread across continents and pose serious health risks. Early and accurate identification of the causative agents is necessary to contain outbreaks. In this report, we describe sequencing-by-hybridization (SBH) technique using high density microarrays to identify Ebola and Marburg viruses. The microarrays were designed to interrogate the sequences of entire viral genomes, and were evaluated with three species of Ebolavirus (Reston, Sudan, and Zaire), and three strains of Marburgvirus (Angola, Musoke, and Ravn). The results showed that the consensus sequences generated with four or more hybridizations had 92.1-98.9% accuracy over 95-99% of the genomes. Additionally, with SBH microarrays it was possible to distinguish between different strains of the Lake Victoria Marburgvirus. J. Med. Virol. 88:1303-1308, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Medicine, Johns Hopkins University, Baltimore, Maryland.
authors.forename: Justin
authors.initials: J
authors.lastname: Hardick
authors.affiliation: Bundeswehr Institute of Microbiology, Munich, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Woelfel
authors.affiliation: United States Army Research Development and Engineering Command, Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland.
authors.forename: Warren
authors.initials: W
authors.lastname: Gardner
authors.affiliation: United States Army Research Development and Engineering Command, Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland.
authors.forename: Sofi
authors.initials: S
authors.lastname: Ibrahim
mesh_terms: D000354:Africa, Western / Q000453:epidemiology; D000810:Angola / Q000453:epidemiology; D000818:Animals; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D004196:Disease Outbreaks; D029043:Ebolavirus / Q000235:genetics*; D016679:Genome, Viral; D019142:Hemorrhagic Fever, Ebola / Q000453:epidemiology / Q000821:virology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008379:Marburg Virus Disease / Q000453:epidemiology; D029024:Marburgvirus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013397:Sudan / Q000453:epidemiology
keywords: Ebola virus; Marburg virus; microarrays