title: Association between FEN1 Polymorphisms -69G>A and 4150G>T with Susceptibility in Human Disease: A Meta-Analysis.
abstract: BACKGROUND
  As a DNA repair protein, flap endonuclease 1 is a key enzyme in maintaining genomic instability and preventing carcinogenesis. Two single nucleotide polymorphisms (SNPs), -69G>A and 4150G>T are associated with DNA damage. This meta-analysis is to evaluate the genetic effects of FEN1 gene SNPs (-69G/A and 4150G/T) and the susceptibility to diseases, including glioma risk, breast cancer, lung cancer, keratoconus (KC) and fuchs' endothelial corneal dystrophy (FECD).
  
  
  METHODS
  A literature search of PubMed and Embase was conducted to identify all eligible published studies. Five case-control studies were included with a total of 5612 cases and 6703 controls in this meta-analysis. Crude odds ratios (ORs) with their corresponding confidence intervals (95%CI) were used to assess the strength of the association.
  
  
  RESULTS
  The FEN1 -69G/A and 4150G/T polymorphisms were significantly associated with the disease risk. Our meta-analysis showed the FEN1 -69GG genotype was correlated to increase risk for the contained diseases compared with the -69AG genotype (OR=0.77, 95%CI=0.71∼0.83). Moreover, the FEN1 4150GG genotype could increase diseases risk compared with the 4150TG genotype (OR=0.81, 95%CI=0.75∼0.87).
  
  
  CONCLUSIONS
  The variant genotypes of the FEN1 -69G/A and FEN1 4150G/T polymorphisms may be associated with diseases susceptibility. However, more studies are needed to detect the disease risk in different ethnic populations.
authors.affiliation: Inst. of Biomedical Engineering, Hangzhou Dianzi University, Hangzhou, China; Inst. of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Nanjiao
authors.initials: N
authors.lastname: Ying
authors.affiliation: Inst. of Biomedical Engineering, Hangzhou Dianzi University, Hangzhou, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Inst. of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Inst. of Biomedical Engineering, Hangzhou Dianzi University, Hangzhou, China.
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Wang
keywords: Disease; Flap endonuclease 1; Genetic variants; SNPs; Susceptibility
references.citation: Mol Cell Biol. 2003 Aug;23(15):5346-53
references.pmid: 12861020
references.citation: EMBO J. 2003 Jul 1;22(13):3451-60
references.pmid: 12840007
references.citation: J Biol Chem. 1997 Feb 21;272(8):4647-50
references.pmid: 9081985
references.citation: Genome Res. 1998 Dec;8(12):1229-31
references.pmid: 9872978
references.citation: Hum Mutat. 2009 Sep;30(9):1320-8
references.pmid: 19618370
references.citation: Mol Cell Biol. 2005 Aug;25(16):6948-55
references.pmid: 16055708
references.citation: Nucleic Acids Res. 2011 Feb;39(3):781-94
references.pmid: 20929870
references.citation: Science. 2002 Jul 26;297(5581):552-7
references.pmid: 12142524
references.citation: Oncogene. 2003 Oct 16;22(46):7243-6
references.pmid: 14562054
references.citation: Biochem Soc Trans. 2010 Apr;38(2):433-7
references.pmid: 20298197
references.citation: Clin Cancer Res. 2005 Jan 15;11(2 Pt 1):473-82
references.pmid: 15701830
references.citation: Am J Hum Genet. 2001 May;68(5):1229-37
references.pmid: 11294658
references.citation: Oncogene. 2011 Mar 3;30(9):1072-81
references.pmid: 20972458
references.citation: Carcinogenesis. 2012 Jan;33(1):119-23
references.pmid: 22072618
references.citation: Bioessays. 2005 Jul;27(7):717-29
references.pmid: 15954100
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9924-9
references.pmid: 12119409
references.citation: Int J Mol Med. 2014 May;33(5):1268-74
references.pmid: 24590400
references.citation: JAMA. 2006 Feb 8;295(6):676-80
references.pmid: 16467236
references.citation: EMBO J. 1994 Mar 1;13(5):1235-46
references.pmid: 8131753
references.citation: J Neurooncol. 2013 Jan;111(2):145-51
references.pmid: 23184144
references.citation: Gene. 2014 Mar 15;538(1):42-5
references.pmid: 24440783
references.citation: Cell. 1997 Jan 24;88(2):253-63
references.pmid: 9008166
references.citation: Stat Med. 2012 Dec 20;31(29):3805-20
references.pmid: 22763950
references.citation: Nat Med. 2007 Jul;13(7):812-9
references.pmid: 17589521
references.citation: Int J Mol Sci. 2014 Aug 22;15(8):14786-802
references.pmid: 25153632
references.citation: Bioessays. 1997 Mar;19(3):233-40
references.pmid: 9080773
references.citation: J Biol Chem. 2015 Jun 12;290(24):15133-45
references.pmid: 25922071
references.citation: EMBO Rep. 2005 Jan;6(1):83-9
references.pmid: 15592449