title: MicroRNA-377 inhibits non-small-cell lung cancer through targeting AEG-1.
abstract: Non-small-cell lung cancer (NSCLC) is the leading cause of cancer-related deaths. MicroRNAs (miRNAs) have been reported to be involved in tumorigenesis. However, the underlying mechanisms of microRNA-377 (miR-377) in NSCLC remain unknown. Hence, in the present study, we aimed to explore the role of miR-377 in the development of NSCLC, with identifying its target genes. The results showed that miR-377 expression was significantly decreased in NSCLC tissues as well as in NSCLC cell lines. Moreover, high expression of miR-377 could markedly inhibit the viability, proliferation, migration and invasion of NSCLC cells. The bioinformatics analysis results showed that astrocyte elevated gene-1 (AEG-1), an oncogene as previously reported, was a potential target gene of miR-377, which was further validated by dual-luciferase reporter assay. Besides, the expression of AEG-1 in protein level was decreased by miR-377 overexpression, but not in mRNA level. In addition, AEG-1 overexpression could reverse the inhibitory effects on NSCLC cells caused by miR-377 transfection. In conclusion, our results suggested that miR-377 played an important role in the development of NSCLC by regulating AEG-1 and may be a potential therapeutic target for NSCLC.
authors.affiliation: Department of Oncology, Tianjin Medical University General Hospital No. 154, Anshan Road, Heping District, Tianjin 300052, P. R. China.
authors.forename: Fanlu
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of Oncology, Tianjin Medical University General Hospital No. 154, Anshan Road, Heping District, Tianjin 300052, P. R. China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Tianjin Medical University General Hospital No. 154, Anshan Road, Heping District, Tianjin 300052, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Oncology, Tianjin Medical University General Hospital No. 154, Anshan Road, Heping District, Tianjin 300052, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Oncology, Tianjin Medical University General Hospital No. 154, Anshan Road, Heping District, Tianjin 300052, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D014162:Transfection
chemical_list: D015815:Cell Adhesion Molecules; C534063:MIRN377 microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: AEG-1; Non-small-cell lung cancer; miR-377; miRNAs; proliferation
references.citation: Oncogene. 2014 Jun 12;33(24):3119-28
references.pmid: 23851509
references.citation: Adv Cancer Res. 2013;120:1-38
references.pmid: 23889986
references.citation: BMC Cancer. 2014;14:703
references.pmid: 25249344
references.citation: Neuro Oncol. 2014 Nov;16(11):1510-22
references.pmid: 24951112
references.citation: Tumour Biol. 2014 Oct;35(10):10019-23
references.pmid: 25012245
references.citation: Med Oncol. 2015 Jan;32(1):361
references.pmid: 25432696
references.citation: Eur J Cell Biol. 2015 Jan;94(1):22-31
references.pmid: 25484183
references.citation: PLoS One. 2015;10(3):e0117714
references.pmid: 25739101
references.citation: Clin Sci (Lond). 2015 May 1;128(10):619-34
references.pmid: 25760961
references.citation: Biomed Pharmacother. 2015 Mar;70:64-71
references.pmid: 25776481
references.citation: BMC Cancer. 2015;15:107
references.pmid: 25880337
references.citation: Mol Cancer. 2015;14:68
references.pmid: 25889255
references.citation: Mol Cancer. 2015;14:73
references.pmid: 25889562
references.citation: Cancer Lett. 2015 Jul 1;362(2):208-17
references.pmid: 25843294
references.citation: Eur J Med Res. 2015;20:50
references.pmid: 25903369
references.citation: Tumour Biol. 2015 May;36(5):3911-7
references.pmid: 25577249
references.citation: Tumour Biol. 2015 Jun;36(6):4357-65
references.pmid: 25874488
references.citation: Tumour Biol. 2015 Aug;36(8):6497-505
references.pmid: 25833694
references.citation: Oncogene. 2002 May 16;21(22):3592-602
references.pmid: 12032861
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: Genome Res. 2004 Oct;14(10A):1902-10
references.pmid: 15364901
references.citation: RNA. 2005 Dec;11(12):1753-61
references.pmid: 16314451
references.citation: Oncogene. 2006 Oct 9;25(46):6163-9
references.pmid: 17028595
references.citation: Nat Rev Cancer. 2006 Nov;6(11):857-66
references.pmid: 17060945
references.citation: Pharmacol Ther. 2007 May;114(2):155-70
references.pmid: 17397930
references.citation: Virchows Arch. 2008 Jan;452(1):1-10
references.pmid: 18040713
references.citation: Nat Cell Biol. 2009 Mar;11(3):228-34
references.pmid: 19255566
references.citation: J Pathol. 2009 Nov;219(3):317-26
references.pmid: 19644957
references.citation: CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90
references.pmid: 21296855
references.citation: Med Oncol. 2011 Jun;28(2):455-62
references.pmid: 20300973
references.citation: Cell. 2012 Mar 16;148(6):1172-87
references.pmid: 22424228
references.citation: Med Oncol. 2013 Mar;30(1):383
references.pmid: 23307243
references.citation: PLoS One. 2013;8(1):e53906
references.pmid: 23372675
references.citation: Trends Mol Med. 2014 Aug;20(8):460-9
references.pmid: 25027972