title: Rapid Identification of Candidate Genes for Seed Weight Using the SLAF-Seq Method in Brassica napus.
abstract: Seed weight is a critical and direct trait for oilseed crop seed yield. Understanding its genetic mechanism is of great importance for yield improvement in Brassica napus breeding. Two hundred and fifty doubled haploid lines derived by microspore culture were developed from a cross between a large-seed line G-42 and a small-seed line 7-9. According to the 1000-seed weight (TSW) data, the individual DNA of the heaviest 46 lines and the lightest 47 lines were respectively selected to establish two bulked DNA pools. A new high-throughput sequencing technology, Specific Locus Amplified Fragment Sequencing (SLAF-seq), was used to identify candidate genes of TSW in association analysis combined with bulked segregant analysis (BSA). A total of 1,933 high quality polymorphic SLAF markers were developed and 4 associated markers of TSW were procured. A hot region of ~0.58 Mb at nucleotides 25,401,885-25,985,931 on ChrA09 containing 91 candidate genes was identified as tightly associated with the TSW trait. From annotation information, four genes (GSBRNA2T00037136001, GSBRNA2T00037157001, GSBRNA2T00037129001 and GSBRNA2T00069389001) might be interesting candidate genes that are highly related to seed weight.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Geng
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
authors.forename: Chenghong
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wu
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wei
mesh_terms: D000483:Alleles; D029688:Brassica napus / Q000033:anatomy & histology / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000737:chemistry*; D003433:Crosses, Genetic; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D005819:Genetic Markers; D006238:Haploidy; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D010641:Phenotype; D000069600:Plant Breeding; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000033:anatomy & histology / Q000235:genetics*
chemical_list: D005819:Genetic Markers
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