title: Acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in Bacillus amyloliquefaciens.
abstract: Lysine acetylation is a major post-translational modification that plays an important regulatory role in almost every aspects in both eukaryotes and prokaryotes. Bacillus amyloliquefaciens, a Gram-positive bacterium, is very effective for the control of plant pathogens. However, very little is known about the function of lysine acetylation in this organism. Here, we conducted the first lysine acetylome in B. amyloliquefaciens through a combination of highly sensitive immune-affinity purification and high-resolution LC-MS/MS. Overall, we identified 3268 lysine acetylation sites in 1254 proteins, which account for 32.9% of the total proteins in this bacterium. Till date, this is the highest ratio of acetylated proteins that have been identified in bacteria. Acetylated proteins are associated with a variety of biological processes and a large fraction of these proteins are involved in metabolism. Interestingly, for the first time, we found that about 71.1% (27/38) and 78.6% (22/28) of all the proteins tightly related to the synthesis of three types of pepketides and five families of lipopeptides were acetylated, respectively. These findings suggest that lysine acetylation plays a critical role in the regulation of antibiotics biosynthesis. These data serves as an important resource for further elucidation of the physiological role of lysine acetylation in B. amyloliquefaciens.
authors.affiliation: Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Song
authors.affiliation: The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Binna
authors.initials: B
authors.lastname: Lv
authors.affiliation: The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Liang
mesh_terms: D000107:Acetylation; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000069977:Bacillus amyloliquefaciens / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008239:Lysine
references.citation: J Proteome Res. 2014 Jul 3;13(7):3294-302
references.pmid: 24874924
references.citation: J Proteome Res. 2013 Sep 6;12(9):3952-68
references.pmid: 23901841
references.citation: Trends Microbiol. 2008 Mar;16(3):115-25
references.pmid: 18289856
references.citation: Int J Biochem Cell Biol. 2015 Feb;59:193-202
references.pmid: 25456444
references.citation: Science. 2010 Feb 19;327(5968):1000-4
references.pmid: 20167786
references.citation: Mol Cell Proteomics. 2009 Feb;8(2):215-25
references.pmid: 18723842
references.citation: J Bacteriol. 2004 Feb;186(4):1084-96
references.pmid: 14762003
references.citation: Annu Rev Microbiol. 2004;58:453-88
references.pmid: 15487945
references.citation: Nat Prod Rep. 2007 Oct;24(5):1073-109
references.pmid: 17898898
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1506-9
references.pmid: 17234808
references.citation: J Biotechnol. 2009 Mar 10;140(1-2):38-44
references.pmid: 19061923
references.citation: Med Res Rev. 2016 Jan;36(1):4-31
references.pmid: 24866700
references.citation: J Bacteriol. 2006 Jun;188(11):4024-36
references.pmid: 16707694
references.citation: Appl Environ Microbiol. 2005 Aug;71(8):4577-84
references.pmid: 16085851
references.citation: J Proteome Res. 2013 Feb 1;12(2):844-51
references.pmid: 23294111
references.citation: Chembiochem. 2010 Jul 26;11(11):1501-4
references.pmid: 20578118
references.citation: J Proteomics. 2014 Jun 25;106:260-9
references.pmid: 24768905
references.citation: Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1786-801
references.pmid: 20817842
references.citation: Int J Mol Sci. 2010;12(1):141-72
references.pmid: 21339982
references.citation: J Environ Sci (China). 2011 Jun;23 Suppl:S63-5
references.pmid: 25084596
references.citation: Appl Microbiol Biotechnol. 2015 Feb;99(3):1399-413
references.pmid: 25487885
references.citation: J Environ Manage. 2013 Sep 30;127:96-102
references.pmid: 23685270
references.citation: Microb Cell Fact. 2009;8:63
references.pmid: 19941639
references.citation: Proteomics. 2013 Aug;13(15):2278-82
references.pmid: 23696451
references.citation: Mol Cell Proteomics. 2012 Nov;11(11):1510-22
references.pmid: 22865919
references.citation: Cell Rep. 2013 Aug 29;4(4):842-51
references.pmid: 23954790
references.citation: J Proteomics. 2013 Feb 21;79:60-71
references.pmid: 23234799
references.citation: Curr Opin Biotechnol. 2007 Dec;18(6):513-20
references.pmid: 17997093
references.citation: Proteomics. 2015 Jul;15(13):2152-7
references.pmid: 25900529
references.citation: Appl Environ Microbiol. 2006 May;72(5):3448-57
references.pmid: 16672490
references.citation: J Proteome Res. 2015 Feb 6;14(2):1275-86
references.pmid: 25621733
references.citation: Proteomics. 2013 May;13(10-11):1726-36
references.pmid: 23468065
references.citation: FEMS Microbiol Rev. 2010 Nov;34(6):1037-62
references.pmid: 20412310
references.citation: Environ Microbiol. 2007 Apr;9(4):1084-90
references.pmid: 17359279
references.citation: Chem Rev. 1997 Nov 10;97(7):2463-2464
references.pmid: 11851465