title: Characterization and Inducing Melanoma Cell Apoptosis Activity of Mannosylerythritol Lipids-A Produced from Pseudozyma aphidis.
abstract: Mannosylerythritol lipids (MELs) are natural glycolipid biosurfactants which have potential applications in the fields of food, cosmetic and medicine. In this study, MELs were produced from vegetable oil by Pseudozyma aphidis. Their structural data through LC/MS, GC/MS and NMR analysis revealed that MEL-A with two acetyls was the major compound and the identified homologs of MEL-A contained a length of C8 to C14 fatty acid chains. This glycolipid exhibited a surface tension of 27.69 mN/m at a critical micelle concentration (CMC), self-assembling into particles in the water solution. It was observed to induce cell growth-inhibition and apoptosis of B16 melanoma cells in a dose-dependent manner, as well as cause cell cycle arrest at the S phase. Further quantitative RT-PCR analysis and western blotting revealed an increasing tendency of both mRNA and protein expressions of Caspase-12, CHOP, GRP78 and Caspase-3, and a down-regulation of protein Bcl-2. Combined with the up regulation of signaling IRE1 and ATF6, it can be speculated that MEL-A-induced B16 melanoma cell apoptosis was associated with the endoplasmic reticulum stress (ERS).
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Fan
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Hongji
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Yongwu
authors.initials: Y
authors.lastname: Niu
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P. R. China.|Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Qihe
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002853:Chromatography, Liquid; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006017:Glycolipids / Q000494:pharmacology*; D013058:Mass Spectrometry; D008546:Melanoma, Experimental / Q000235:genetics / Q000473:pathology*; D051379:Mice; D041681:NIH 3T3 Cells; D066244:Proton Magnetic Resonance Spectroscopy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012996:Solutions; D014587:Ustilaginales / Q000737:chemistry*; D014867:Water
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D005227:Fatty Acids; D006017:Glycolipids; C000717248:Hspa5 protein, mouse; D012333:RNA, Messenger; D012996:Solutions; C439671:mannosylerythritol lipid; D014867:Water
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