title: Meeting report: GARNet/OpenPlant CRISPR-Cas workshop.
abstract: Targeted genome engineering has been described as a "game-changing technology" for fields as diverse as human genetics and plant biotechnology. One technique used for precise gene editing utilises the CRISPR-Cas system and is an effective method for genetic engineering in a wide variety of plants. However, many researchers remain unaware of both the technical challenges that emerge when using this technique or of its potential benefits. Therefore in September 2015, GARNet and OpenPlant organized a two-day workshop at the John Innes Centre that provided both background information and hands-on training for this important technology.
authors.affiliation: GARNet, School of Biosciences, Cardiff University, Cardiff, UK.
authors.forename: Geraint
authors.initials: G
authors.lastname: Parry
authors.affiliation: OpenPlant, John Innes Centre, Norwich Research Park, Norwich, UK.|Synthetic Biology Laboratory, The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Norwich, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Patron
authors.affiliation: GARNet, School of Biosciences, Cardiff University, Cardiff, UK.
authors.forename: Ruth
authors.initials: R
authors.lastname: Bastow
authors.affiliation: OpenPlant, John Innes Centre, Norwich Research Park, Norwich, UK.
authors.forename: Colette
authors.initials: C
authors.lastname: Matthewman
keywords: CRISPR; Cas9; Gene Editing; Genetic Engineering
references.citation: Plant Biotechnol J. 2016 Feb;14(2):510-8
references.pmid: 26251102
references.citation: New Phytol. 2015 Oct;208(1):13-9
references.pmid: 26171760
references.citation: Genome Biol. 2015 Nov 30;16:258
references.pmid: 26616834
references.citation: Nat Biotechnol. 2013 Aug;31(8):691-3
references.pmid: 23929340
references.citation: ACS Synth Biol. 2014 Nov 21;3(11):839-43
references.pmid: 24933124
references.citation: PLoS Biol. 2014 Jun 10;12(6):e1001877
references.pmid: 24915127
references.citation: Science. 2014 Jan 3;343(6166):80-4
references.pmid: 24336569
references.citation: PLoS Genet. 2015 May 28;11(5):e1005084
references.pmid: 26020919
references.citation: Public Underst Sci. 2010 Jan;19(1):98-114
references.pmid: 20533793
references.citation: Plant J. 2014 Jul;79(2):348-59
references.pmid: 24836556
references.citation: PLoS One. 2013 Jun 05;8(6):e64879
references.pmid: 23755155
references.citation: Nucleic Acids Res. 2014;42(17):10903-14
references.pmid: 25200087
references.citation: Bioeng Bugs. 2012 Jan 1;3(1):38-43
references.pmid: 22126803
references.citation: Biotechnol Adv. 2015 Jan-Feb;33(1):41-52
references.pmid: 25536441
references.citation: Nat Biotechnol. 2013 Aug;31(8):688-91
references.pmid: 23929339
references.citation: Nat Biotechnol. 2013 Sep;31(9):833-8
references.pmid: 23907171
references.citation: Plant Methods. 2013 Oct 11;9(1):39
references.pmid: 24112467
references.citation: Plant Physiol. 2015 Oct;169(2):931-45
references.pmid: 26269544
references.citation: Nucleic Acids Res. 1993 Nov 11;21(22):5034-40
references.pmid: 8255757