title: Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines.
abstract: Although human pluripotent stem cells (hPSCs) can proliferate robustly on the feeder-free culture system, genetic instability of hPSCs has been reported in such environment. Alternatively, feeder cells enable hPSCs to maintain their pluripotency. The feeder cells are usually grown in a culture medium containing fetal bovine serum (FBS) prior to coculture with hPSCs. The use of FBS might limit the clinical application of hPSCs. Recently, human cord blood-derived serum (hUCS) showed a positive effect on culture of mesenchymal stem cells. It is interesting to test whether hUCS can be used for culture of feeder cells of hPSCs. This study was aimed to replace FBS with hUCS for culturing the human foreskin fibroblasts (HFFs) prior to feeder cell preparation. The results showed that HFFs cultured in hUCS-containing medium (HFF-hUCS) displayed fibroblastic features, high proliferation rates, short population doubling times, and normal karyotypes after prolonged culture. Inactivated HFF-hUCS expressed important genes, including Activin A, FGF2, and TGFβ1, which have been implicated in the maintenance of hPSC pluripotency. Moreover, hPSC lines maintained pluripotency, differentiation capacities, and karyotypic stability after being cocultured for extended period with inactivated HFF-hUCS. Therefore, the results demonstrated the benefit of hUCS for hPSCs culture system.
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Ruttachuk
authors.initials: R
authors.lastname: Rungsiwiwut
authors.affiliation: Stem Cell and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Praewphan
authors.initials: P
authors.lastname: Ingrungruanglert
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pranee
authors.initials: P
authors.lastname: Numchaisrika
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pramuan
authors.initials: P
authors.lastname: Virutamasen
authors.affiliation: Stem Cell Research and Development Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Tatsanee
authors.initials: T
authors.lastname: Phermthai
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Kamthorn
authors.initials: K
authors.lastname: Pruksananonda
references.citation: Int J Dev Biol. 2008;52(4):353-63
references.pmid: 18415935
references.citation: Tissue Eng Part A. 2010 Nov;16(11):3467-84
references.pmid: 20572797
references.citation: J Cell Mol Med. 2012 Jan;16(1):72-82
references.pmid: 21418515
references.citation: J Biol Chem. 2013 Jun 28;288(26):18716-31
references.pmid: 23658023
references.citation: PLoS One. 2012;7(6):e35325
references.pmid: 22745653
references.citation: Cell Biol Int. 2006 Dec;30(12):1041-7
references.pmid: 17074515
references.citation: Science. 1998 Nov 6;282(5391):1145-7
references.pmid: 9804556
references.citation: Stem Cells. 2006 Oct;24(10):2170-6
references.pmid: 16741223
references.citation: Stem Cells Dev. 2010 Apr;19(4):547-56
references.pmid: 19883201
references.citation: Blood Cells. 1994;20(2-3):492-7
references.pmid: 7749116
references.citation: Thromb Haemost. 2015 Apr;113(4):792-805
references.pmid: 25518736
references.citation: Hum Reprod. 2009 Oct;24(10):2567-81
references.pmid: 19556288
references.citation: Int Rev Cell Mol Biol. 2011;292:153-96
references.pmid: 22078961
references.citation: Stem Cells. 2007 May;25(5):1270-8
references.pmid: 17255520
references.citation: Lancet. 2015 Feb 7;385(9967):509-16
references.pmid: 25458728
references.citation: Mol Reprod Dev. 2008 Oct;75(10):1523-32
references.pmid: 18318041
references.citation: Stem Cells Dev. 2012 Jul 20;21(11):2036-48
references.pmid: 22149941
references.citation: Biores Open Access. 2012 Aug;1(4):166-73
references.pmid: 23514952
references.citation: Nat Biotechnol. 2006 Feb;24(2):185-7
references.pmid: 16388305
references.citation: Cell Biol Int. 2007 Mar;31(3):293-8
references.pmid: 17208468
references.citation: Lancet. 2012 Feb 25;379(9817):713-20
references.pmid: 22281388
references.citation: Mol Cancer. 2008 Oct 03;7:76
references.pmid: 18834512
references.citation: Cytotherapy. 2012 Sep;14(8):948-53
references.pmid: 22587592
references.citation: Cytotherapy. 2014 May;16(5):683-91
references.pmid: 24119645
references.citation: Stem Cell Res Ther. 2012 Mar 28;3(2):12
references.pmid: 22472092
references.citation: Stem Cells. 2005 Apr;23(4):544-9
references.pmid: 15790775
references.citation: Acta Medica (Hradec Kralove). 2013;56(4):142-9
references.pmid: 24693795
references.citation: Int J Clin Exp Pathol. 2014 Jul 15;7(8):4661-73
references.pmid: 25197338
references.citation: Head Neck. 2014 Mar;36(3):401-10
references.pmid: 23765508
references.citation: J Cell Biochem. 2011 May;112(5):1353-63
references.pmid: 21337383
references.citation: Cell. 2007 Nov 30;131(5):861-72
references.pmid: 18035408
references.citation: Nat Rev Cancer. 2011 Apr;11(4):268-77
references.pmid: 21390058
references.citation: Hum Reprod. 2003 Jul;18(7):1404-9
references.pmid: 12832363
references.citation: Stem Cells Dev. 2009 May;18(4):559-71
references.pmid: 18754716
references.citation: PLoS One. 2013 Oct 02;8(10):e76205
references.pmid: 24098444