title: Production of a therapeutic protein by fusing it with two fragments of the carboxyl-terminal peptide of human chorionic gonadotropin β-subunit in Pichia pastoris.
abstract: OBJECTIVE
  To produce a therapeutic protein (endostatin) by fusion with two fragments of the carboxyl-terminal peptide (CTP) of the human chorionic gonadotropin β-subunit in Pichia pastoris.
  
  
  RESULTS
  Two CTP sequences were fused to the C-terminal of human endostatin, and the fusion protein (endo-CTP) was expressed by P. pastoris. Endo-CTP inhibited proliferation of endothelial cells with an IC50 of 7 μg ml(-1), and 30 % of cells were annexin V-positive after treatment with 20 μg endo-CTP ml(-1) for 48 h. Migration of endothelial cells was inhibited by endo-CTP in a concentration-dependent manner. The half-life of endo-CTP in Sprague-Dawley rats was much longer than that of its commercial counterpart (Endostar).
  
  
  CONCLUSIONS
  A long-acting endostatin can be produced using CTP technology.
authors.affiliation: Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China.
authors.forename: Baolong
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biochemical Engineer, Faculty of Engineer, University College London, London, WC1E 6BT, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Wayne
authors.affiliation: Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China.
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.affiliation: Shandong Simcere-Medgenn Bio-pharmaceutical Co., Ltd., Yantai, 264000, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Shandong Simcere-Medgenn Bio-pharmaceutical Co., Ltd., Yantai, 264000, People's Republic of China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China. fmq@fudan.edu.cn.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Feng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism* / Q000493:pharmacokinetics; D049109:Cell Proliferation / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000235:genetics / Q000378:metabolism*; D043169:Endostatins / Q000235:genetics / Q000378:metabolism* / Q000493:pharmacokinetics; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006207:Half-Life; D020128:Inhibitory Concentration 50; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D006063:Chorionic Gonadotropin; D043169:Endostatins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins
keywords: Cancer angiogenesis; Carboxyl-terminal peptide; Endostatin; Endothelial cells; Pichia pastoris