title: Comparative proteomics of Acidithiobacillus ferrooxidans grown in the presence and absence of uranium.
abstract: Acidithiobacillus ferrooxidans is an acidophile that thrives in metal-contaminated environments and tolerates high levels of uranium. To gain a better understanding of the processes involved in U(VI) resistance, comparative proteomics was used. The proteome of A. ferrooxidans was grown in the presence and absence of 0.5 mM U(VI); expression of 17 proteins was upregulated and one was downregulated. Most proteins with increased expression are part of the general stress response or are involved in reactive oxygen species detoxification. Four novel proteins showed increased expression in the presence of U(VI) and may contribute to U(VI) resistance via thiol homoeostasis and U(VI) binding.
authors.affiliation: Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK. Electronic address: l.dekker@imperial.ac.uk.
authors.forename: Linda
authors.initials: L
authors.lastname: Dekker
authors.affiliation: Génétique Moléculaire, Génomique et Microbiologie, UMR 7156 CNRS and Université de Strasbourg, 28 rue Goethe, 67000 Strasbourg, France. Electronic address: ploetze@unistra.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Arsène-Ploetze
authors.affiliation: Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK. Electronic address: j.santini@ucl.ac.uk.
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Santini
mesh_terms: D042763:Acidithiobacillus / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D004361:Drug Tolerance; D020543:Proteome / Q000032:analysis*; D014501:Uranium / Q000378:metabolism*
chemical_list: D020543:Proteome; D014501:Uranium
keywords: Acidithiobacillus ferrooxidans; Acidophile; Proteome; Stress; Uranium resistance