title: Methods to Study Metastasis in Genetically Modified Mice.
abstract: Metastasis is often modeled by xenotransplantation of cell lines in immunodeficient mice. A wealth of information about tumor cell behavior in the new environment is obtained from these efforts. Yet by design, this approach is "tumor-centric," as it focuses on cell-autonomous determinants of human tumor dissemination in mouse tissues, in effect using the animal body as a sophisticated "Petri dish" providing nutrients and support for tumor growth. Transgenic or gene knockout mouse models of cancer allow the study of tumor spread as a systemic disease and offer a complimentary approach for studying the natural history of cancer. This introduction is aimed at describing the overall methodological approach to studying metastasis in genetically modified mice, with a particular focus on using animals with regulated expression of potent human oncogenes in the breast.
authors.affiliation: Institut de Recherches Cliniques de Montréal, Montréal, Quebec H2W 1R7, Canada; Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 1A3, Canada;
authors.forename: Farhia
authors.initials: F
authors.lastname: Kabeer
authors.affiliation: Department of Medicine, University of Louisville, Louisville, Kentucky 40202; Department of Pharmacology and Toxicology, University of Louisville, Louisville, Kentucky 40202;
authors.forename: Levi J
authors.initials: LJ
authors.lastname: Beverly
authors.affiliation: Université Pierre et Marie Curie, Université Paris 06, Unité Mixte de Recherche 7211; INSERM, Unité Mixte de Researche S959, R3, F-75013 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Hôpital Necker-Enfants Malades, Faculté de Médecine Paris Desecartes; INSERM, Unité 1013, F-75015 Paris, France; Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Darrasse-Jèze
authors.affiliation: Institut de Recherches Cliniques de Montréal, Montréal, Quebec H2W 1R7, Canada; Pathologie et Biologie Cellulaire, Université de Montréal, Montréal, Quebec H3T 1J4, Canada; Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 1A3, Canada;
authors.forename: Katrina
authors.initials: K
authors.lastname: Podsypanina
mesh_terms: D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D009362:Neoplasm Metastasis / Q000473:pathology*