title: Level of circulated microRNA-421 in gastric carcinoma and related mechanisms.
abstract: As one of the most popular and deadly malignant tumors, gastric cancer still has difficulty in early-diagnosis. Recently the level of circulated DNA related with tumors can be used for diagnosis. MicroRNA-421 (miR-421) has been found to be up-regulated in tumor cells. Whether peripheral miR-421 can be used as a marker for diagnosis of gastric carcinoma, however, remains unclear. The expression level of miR-421 in both gastric cancer and normal people were firstly quantified. We then performed in vitro transfection of gastric carcinoma cell line to potentiate or silence miR-421 level. Cell apoptosis and apoptotic protein levels were quantified by flow cytometry and Western blotting, respectively. MiR-421 level in the peripheral blood of gastric cancer patients was significantly elevated. In gastric cancer cell line, the up-regulation of miR-421 significantly inhibited cell apoptosis. The silencing of miR-421 promoted cell apoptosis. Such anti-apoptotic role of miR-421 was accomplished by inhibiting caspase 3, up-regulating Bcl-2 and inhibiting Bax. MiR-421 was up-regulated in both tumor tissue and peripheral blood, and can modulate cell apoptosis. Circulated miR-421 can work as a serological marker for early diagnosis of gastric cancer.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Obstetrics, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Hui
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002277:Carcinoma / Q000097:blood* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D055088:Early Detection of Cancer; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D011237:Predictive Value of Tests; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D014162:Transfection; D015854:Up-Regulation
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C547665:MIRN421 microRNA, human; D035683:MicroRNAs
keywords: Bax and Bcl-2; Gastric carcinoma; caspase 3; cell apoptosis; circulated microRNA-421
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