title: Gene expression profiling and mechanism study of neural stem cells response to surface chemistry.
abstract: To declare the mechanisms of neural stem cells (NSCs) in response to material surface chemistry, NSCs were exposed to the self-assemble monolayers of alkanethiolates on gold surfaces terminated with amine (NH2), hydroxyl (OH) and methyl (CH3) for analysis. The morphological responses of NSCs were recorded; the gene expression profilings were detected by genechips; the gene expressions data of NSCs responded to different chemical groups were declared through the gene ontology term and pathway analyses. It showed that cells behaved dissimilar on the three chemical groups, the adhesion, proliferation and migration were easier on the NH2 and OH groups; the gene expressions of NSCs were induced differently, either, involved in several functional processes and signaling pathways. CH3 group induced genes enriched much in chemistry reactions and death processes, whereas many genes of cellular nucleotide metabolism were down-regulated. NH2 group induced NSCs to express many genes of receptors on membrane, and participated in cellular signal transduction of cell adhesion and interactions, or associated with axon growth. OH group was similar to NH2 group to induce the membrane response, but it also down regulated metabolism of cells. Therefore, it declared the chemical groups affected NSCs through inner way and the NH2, OH and CH3 groups triggered the cellular gene expression in different signaling pathways.
authors.affiliation: Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
authors.forename: Shenglian
authors.initials: S
authors.lastname: Yao
authors.affiliation: Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yu
authors.affiliation: Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
keywords: biomaterial; chemical group; gene expression; neural stem cell; receptor; signaling pathway
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