title: Human mesenchymal and murine stromal cells support human lympho-myeloid progenitor expansion but not maintenance of multipotent haematopoietic stem and progenitor cells.
abstract: A major goal in haematopoietic stem cell (HSC) research is to define conditions for the expansion of HSCs or multipotent progenitor cells (MPPs). Since human HSCs/MPPs cannot be isolated, NOD/SCID repopulating cell (SRC) assays emerged as the standard for the quantification of very primitive haematopoietic cell. However, in addition to HSCs/MPPs, lympho-myeloid primed progenitors (LMPPs) were recently found to contain SRC activities, challenging this assay as clear HSC/MPP readout. Because our revised model of human haematopoiesis predicts that HSCs/MPPs can be identified as CD133(+)CD34(+) cells containing erythroid potentials, we investigated the potential of human mesenchymal and conventional murine stromal cells to support expansion of HSCs/MPPs. Even though all stromal cells supported expansion of CD133(+)CD34(+) progenitors with long-term myeloid and long-term lymphoid potentials, erythroid potentials were exclusively found within erythro-myeloid CD133(low)CD34(+) cell fractions. Thus, our data demonstrate that against the prevailing assumption co-cultures on human mesenchymal and murine stromal cells neither promote expansion nor maintenance of HSCs and MPPs.
authors.affiliation: a Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen , Essen , Germany.|b Clinical Research Division, Fred Hutchinson Cancer Research Center , Seattle , WA , USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Radtke
authors.affiliation: a Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen , Essen , Germany.
authors.forename: André
authors.initials: A
authors.lastname: Görgens
authors.affiliation: c 307-Ivy Translational Medicine Center, Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences , Beijing , China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: a Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen , Essen , Germany.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Horn
authors.affiliation: a Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen , Essen , Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Giebel
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D018920:Coculture Techniques / Q000379:methods*; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000378:metabolism
keywords: CD133; EMPs; HSCs; LMPPs; MPPs; MSCs; Prominin-1; haematopoiesis; hematopoiesis
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