title: Amiloride suppresses pilocarpine-induced seizures via ASICs other than NHE in rats.
abstract: OBJECTIVE
  Although recent studies have indicated that acid-sensing ion channels (ASICs) may play an important role in suppressing status epilepticus (SE) in rats, the precise mechanism is unclear. We attempted to investigate the antiepileptic effect of amiloride in SE rats and its mechanism.
  
  
  METHODS
  Rats with seizures induced by Li-pilocarpine were randomly divided into four groups, phosphate buffer saline (PBS) group, amiloride group, levetiracetam group and acidic liquid group, respectively. The electroencephalogram (EEG) of each group was recorded. Then rats treated with different drugs (2 h after amiloride or PBS injection or 1 h after PBS injection) and a normal control group was selected for reverse transcription-polymerase chain reaction (RT-PCR). The expression of ASIC1a, ASIC3 and sodium-hydrogen exchanger (NHE) in each group was detected.
  
  
  RESULTS
  Amiloride reduced the frequency of discharge in 60~90 min after injection significantly. In acidic liquid group, the epileptic discharge was increased in 0~30 min. Moreover, the expression of ASIC1a, ASIC3 and NHE was obviously increased in the SE groups. Compared with SE groups, the expression of ASIC1a and ASIC3 mRNA in amiloride group decreased significantly. While NHE mRNA expression in the SE groups showed no significant difference.
  
  
  CONCLUSIONS
  Amiloride inhibited pilocarpine-induced SE and the anti-epileptic mechanism was associated with deactivation of the ASIC1a and ASIC3 instead of NHE in rats.
authors.affiliation: Department of Neurology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. C.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liang
authors.affiliation: Department of Neurology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. C.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Huang
authors.affiliation: Department of Neurology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. C.
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Chen
authors.affiliation: Department of Neurology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. C.
authors.forename: Song-Qing
authors.initials: SQ
authors.lastname: Pan
authors.affiliation: Department of Neurology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. C.
authors.forename: Zu-Neng
authors.initials: ZN
authors.lastname: Lu
authors.affiliation: Department of Neurology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. C.
authors.forename: Zhe-Man
authors.initials: ZM
authors.lastname: Xiao
mesh_terms: D062646:Acid Sensing Ion Channel Blockers / Q000494:pharmacology*; D062565:Acid Sensing Ion Channels / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D004569:Electroencephalography; D008297:Male; D010862:Pilocarpine; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D017923:Sodium-Hydrogen Exchangers / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: C568269:ASIC3 protein, rat; D062646:Acid Sensing Ion Channel Blockers; D062565:Acid Sensing Ion Channels; D000927:Anticonvulsants; C568268:Asic1 protein, rat; D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers; D010862:Pilocarpine; D000584:Amiloride
keywords: ASICs; Amiloride; EEG; NHE; epilepsy; pilocarpine
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