title: The E-selectin S149R polymorphisms in breast cancer in a northern Iran population.
abstract: Breast cancer is a complex polygenic disorder that genetic factors play an important role in disease etiology. E-selectin mediates the adhesion of tumor cells to endothelial cells. This interaction is associated with metastatic dissemination. Expression of E-selectin on the endothelium is a hallmark of inflammation. This study was performed to evaluate the association of E-selectin S149R polymorphisms and the risk of breast cancer. A total of 100 patients with breast cancer and 120 healthy sample donors (controls) were recruited in this study. Genomic DNA was extracted from fresh blood samples and genotyping of the E-selectin genes was performed using PCR-restriction fragment length polymorphism (PCR-RFLP). The frequencies of the CC, AC and AA genotypes were 2%, 84%, and 14% in patients and 2%, 12%, and 86% in controls, respectively, while the A and C allelic frequency was 56%, 44% in patients and 92%, 8% in controls, respectively. Statistical analysis showed that there is a significant difference between two groups (P<0.0001). It is concluded that the E-selectin S149R polymorphisms is associated with the oncogenesis of breast cancer in a population in northern Iran.
authors.affiliation: University of Guilan Department of Cell and Molecular Biology Rasht Iran.
authors.forename: G
authors.initials: G
authors.lastname: Golnarnik
authors.affiliation: University of Guilan Department of Biology, Faculty of Sciences Rasht Iran mashayekhi@;guilan.ac.ir.
authors.forename: F
authors.initials: F
authors.lastname: Mashayekhi
authors.affiliation: Guilan University of Medical Sciences Faculty of Medicine Rasht Iran.
authors.forename: H S
authors.initials: HS
authors.lastname: Saedi
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001943:Breast Neoplasms / Q000209:etiology* / Q000235:genetics*; D016022:Case-Control Studies; D019040:E-Selectin / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D007492:Iran; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D012307:Risk Factors
chemical_list: D019040:E-Selectin; C546936:SELE protein, human