title: Hypoxia induced upregulation of miR-301a/b contributes to increased cell autophagy and viability of prostate cancer cells by targeting NDRG2.
abstract: OBJECTIVE
  Previous studies reported that NDRG2 might be a tumor suppressor of prostate cancer. In this study, we investigated the hypoxia-induced expression change of miR-301a/b in prostate cancer cells and explored its regulation on NDRG2 in autophagy and viability of prostate cancer cells.
  
  
  METHODS
  MiR-301a/b expression in hypoxia and normoxia cultured prostate cancer cells was measured. Its regulation on autophagy was measured by quantifying expression change of LC3B and p62. The direct binding between miR-301a/b and 3'UTR of NDRG2 was verified using dual luciferase, qRT-PCR and Western blot assay. The influence of miR-301a/b-NDRG2 axis on autophagy, viability and apoptosis of prostate cancer cells was further investigated.
  
  
  RESULTS
  Hypoxia induced a significant upregulation of miR-301a/b in prostate cancer cells. Enhanced miR-301a/b expression significantly weakened autophagy of prostate cancer cells. Both miR-301a and miR-301b could directly target 3'UTR of NDRG2 and decrease its expression. Decreased NDRG2 expression directly resulted in increased autophagy and cell viability and reduced cell apoptosis.
  
  
  CONCLUSIONS
  Taken together, we demonstrated that miR-301a/b-NDRG2 might be an important axis modulating autophagy and viability of prostate cancer cells under hypoxia.
authors.affiliation: Department of Urology, General Hospital of Beijing Military Region, PLA, Beijing, China. yaweiguan1@tom.com.
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Guo
authors.forename: J-X
authors.initials: JX
authors.lastname: Liu
authors.forename: Y-W
authors.initials: YW
authors.lastname: Guan
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001343:Autophagy / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D006801:Humans; D000860:Hypoxia / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000503:physiopathology; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C550735:MIRN301A microRNA, human; D035683:MicroRNAs; C466228:NDRG2 protein, human; D025521:Tumor Suppressor Proteins