title: Vasorelaxation of goat mesenteric artery is mediated by endothelial Na(+)-K(+)-ATPase.
abstract: OBJECTIVE
  To examine the role of Na(+)-K(+)-ATPase and K(+) channels in mediating vasorelaxation in the superior mesenteric artery of Capra hircus.
  
  
  METHODS
  Goat superior mesenteric artery (GSMA) was cut into 1.5-2 mm circular rings and mounted in a thermostatically controlled (37°C ± 0.5°C) organ bath containing 20 ml of modified Krebs-Henseleit saline (MKHS) (pH 7.4), with continuous aeration under 1.5 g tension for 90 min. Endothelium-intact (ED+) or endothelium-denuded (ED-) GSMA ring was contracted with phenylephrine (PE) or 5-hydroxytryptamine (5-HT) (1 μM-0.1 mM) in the absence or presence of ouabain (0.1 μM). KCl (1 μM-10 mM) was added cumulatively to K(+)-free MKHS-pre-contracted (ED+/-) rings in the absence or presence of ouabain (0.1 μM) or barium (1 μM) or 4-aminopyridine (1 μM).
  
  
  RESULTS
  Ouabain did not alter the basal tone of the arterial ring. The contractile response induced by PE (Emax: 50.46 ± 2.68, pD2: 5.53 ± 0.04) and 5-HT (Emax: 30.86 ± 1.33, pD2: 6.17 ± 0.03) in ED+ ring was significantly (P < 0.001) augmented in ED- rings (PE: Emax: 93.30 ± 2.11, pD2: 6.41 ± 0.04; 5-HT: Emax: 95.07 ± 0.99, pD2: 6.27 ± 0.03). The contractile response induced by PE and 5-HT in ED+ or ED- rings in the presence of ouabain was almost identical with that of ED- rings. Vasorelaxation of KCl (Emax: 2.90 ± 1.14, pD2: 3.9 ± 0.03) was significantly attenuated in the presence of ouabain (Emax: 73.8 ± 5.16, pD2: 4.3 ± 0.04), Ba(2+) (Emax: 16.34 ± 4.7, pD2: 3.22 ± 0.02), 4-AP (Emax: 18.16 ± 2.4, pD2: 3.68 ± 0.03), ouabain and Ba(2+) (Emax: 70.09 ± 3.66, pD2: 4.41 ± 0.04), and ouabain and 4-AP (Emax: 66.98 ± 4.61, pD2: 4.13 ± 0.06).
  
  
  CONCLUSIONS
  The vasorelaxation in GSMA is mediated by the endothelium-derived hyperpolarizing factor (EDHFs) such as ouabain-sensitive Na(+)-K(+)-ATPase, KIR and Kv channels.
authors.affiliation: Department of Pharmacology and Toxicology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India.
authors.forename: R
authors.initials: R
authors.lastname: Sathiskumar
authors.affiliation: FREM Division, Central Inland Fisheries Research Institute, ICAR, Kolkata, West Bengal, India.
authors.forename: Bimal Prasanna
authors.initials: BP
authors.lastname: Mohanty
authors.affiliation: Department of Pharmacology and Toxicology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India.
authors.forename: Subas Chandra
authors.initials: SC
authors.lastname: Parija
keywords: EDHF; KIR; KV; goat; mesenteric artery; ouabain; pump; sodium pump
references.citation: Am J Physiol Cell Physiol. 2001 Oct;281(4):C1336-43
references.pmid: 11546672
references.citation: Nature. 1983 Oct 13-19;305(5935):627-30
references.pmid: 6621711
references.citation: Br J Pharmacol. 2002 Feb;135(3):771-81
references.pmid: 11834625
references.citation: Br J Pharmacol. 2002 Jul;136(6):918-26
references.pmid: 12110616
references.citation: Br J Pharmacol. 1999 May;127(1):27-34
references.pmid: 10369452
references.citation: PLoS One. 2014 Sep 04;9(9):e106345
references.pmid: 25187951
references.citation: Chin J Physiol. 2005 Jun 30;48(2):86-92
references.pmid: 16201453
references.citation: Br J Pharmacol. 2000 Jul;130(5):1174-82
references.pmid: 10882404
references.citation: J Physiol. 2005 Nov 15;569(Pt 1):243-56
references.pmid: 16166162
references.citation: J Clin Invest. 1992 Sep;90(3):727-32
references.pmid: 1325996
references.citation: Am J Med Sci. 1993 Mar;305(3):157-63
references.pmid: 8383425
references.citation: Br J Pharmacol. 2011 Oct;164(3):839-52
references.pmid: 21133895
references.citation: Pflugers Arch. 2010 May;459(6):863-79
references.pmid: 20383718
references.citation: Biochim Biophys Acta. 2011 Jun;1808(6):1684-700
references.pmid: 21334305
references.citation: J Hypertens. 2000 Jul;18(7):877-84
references.pmid: 10930185
references.citation: Blood Vessels. 1978;15(1-3):198-207
references.pmid: 147117
references.citation: Braz J Med Biol Res. 2009 Nov;42(11):1058-67
references.pmid: 19820882
references.citation: Circ Res. 1992 Oct;71(4):943-50
references.pmid: 1516165
references.citation: Indian J Pharmacol. 2009 Apr;41(2):68-71
references.pmid: 20336220
references.citation: Am J Physiol Heart Circ Physiol. 2010 Jan;298(1):H263-74
references.pmid: 19897708
references.citation: Br J Pharmacol. 1986 Dec;89(4):819-30
references.pmid: 3028554
references.citation: Br J Pharmacol. 2000 Feb;129(3):605-11
references.pmid: 10711361
references.citation: J Cardiovasc Pharmacol. 1993;22 Suppl 2:S99-101
references.pmid: 7508044
references.citation: Br J Pharmacol. 1988 Oct;95(2):531-43
references.pmid: 2852524
references.citation: Nature. 1971 Jan 29;229(5283):344-5
references.pmid: 4925786
references.citation: Am J Physiol Heart Circ Physiol. 2000 Dec;279(6):H2704-12
references.pmid: 11087224
references.citation: Physiol Rev. 1997 Oct;77(4):1165-232
references.pmid: 9354814
references.citation: Cardiovasc Res. 1998 Mar;37(3):780-90
references.pmid: 9659463
references.citation: J Cardiovasc Pharmacol. 2008 Aug;52(2):167-75
references.pmid: 18670362