title: [The role of cell apoptosis mediated by endoplasmic reticulum stress (ERS) of deep tissue injury of pressure ulcer of rats].
abstract: OBJECTIVE
  To observe the the expression of endoplasmic reticulum stress (ERS) related factors in deep tissue injury (DTI) at pressure ulcer rat and to investigate the ERS mechanism of DTI in muscle tissue and protective effect of 4-phenylbutyric acid (4-PBA) in local tissue.
  
  
  METHODS
  Fifty male SD rats were randomly devided into control group, model group, experimental group NS group and PBA group, the experimental groups were divided into 4 d, 7 d, 14 d and 21 d group according to the observation time (n = 5). Rats in the PBA group were administrated with gastric perfusion of 4-PBA after the modeling; the NS group was given normal saline of the same quantity. Using HE staining to observe morphologic character. The expression of glucose regulated protein 78 (GRP78), CHOP, Caspase 12 were detected by immunohistochernical staining. Cell apoptosis was detected by TUNEL assay.
  
  
  RESULTS
  HE staining results showed that each group demonstrated compression injury compared with control group: cellular swelling, ompaction of nuclear, and apoptosis in muscle tissue. The new muscle fiber in 4-PBA group fused faster than those in NS group. The number of TUNEL positive cells peaked at 4 day after compression, then got decreased on day 7 in muscle tissue, apoptosis positive cells were diminished after 4-PBA treatment. The immunohistochemical staining results showed that the expression of protein GRP78, CHOP, Caspase 12 peakd 4 d after modeling and decreased gradually. The GRP78, CHOP, Caspase 12 protein expression were significantly higher than those of PBA group at all time points (P < 0.05).
  
  
  CONCLUSIONS
  Cell apoptosis induced by endoplasmic reticulum stress took part in deep tissue injury resulting of pressure ulcer, which mechanism might be related to reducing apoptosis mediated by CHOP, Caspase 12.
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Pan
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Mao
authors.forename: Hao-huang
authors.initials: HH
authors.lastname: Xie
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.forename: Xiao-kun
authors.initials: XK
authors.lastname: Li
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Jiang
mesh_terms: D000818:Animals; D017209:Apoptosis; D053454:Caspase 12 / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D006360:Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000473:pathology; D010654:Phenylbutyrates / Q000494:pharmacology; D003668:Pressure Ulcer / Q000503:physiopathology*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D051742:Transcription Factor CHOP / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D053454:Caspase 12; D006360:Heat-Shock Proteins; D010654:Phenylbutyrates; D051742:Transcription Factor CHOP; C497251:Ddit3 protein, rat; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP; C506443:Casp12 protein, rat