title: A higher frequency of IL-10-producing B cells in Hepatitis B virus-associated membranous nephropathy.
abstract: The purpose of this study is to elucidate the potential role of interleukin (IL)-10(+) regulatory B cells and other B cell subsets in the development of hepatitis B virus-associated membranous nephropathy (HBV-MN). A total of 14 patients with new onset HBV-MN, 12 individuals with immune-tolerant HBV infection (HBV-IT), and 12 healthy controls (HC) were examined for the percentages of CD38(+) , CD86(+) , CD27(+) , CD95(+) and IL-10(+) B cells by flow cytometry. Serum IL-10 concentration was examined by enzyme-linked immunosorbent assay (ELISA). The percentages of CD38(+) CD19(+) , CD86(+) CD19(+) , CD38(+) CD86(+) CD19(+) , and CD95(+) CD19(+) B cells were significantly higher in HBV-MN patients than the HBV-IT and HC. The percentages of CD5(+) CD19(+) , IL-10(+) CD19(+) B cells and serum IL-10 level in HBV-MN patients were significantly higher than the HC, and lower than the HBV-IT. Percentages of CD38(+) CD19(+) , and CD86(+) CD19(+) B cells were reduced after treatment, while the percentages of CD5(+) CD1d(+) CD19(+) , CD5(+) CD1d(+) IL-10(+) CD19(+) , and IL-10(+) CD19(+) B cells were increased. The 24 h urinary protein concentration was positively correlated with the percentage of CD38(+) CD19(+) , and negatively correlated with the percentage of IL-10(+) CD19(+) B cells and serum IL-10 level. Similarly, the value of eGFR was negatively correlated with the percentage of CD38(+) CD19(+) , and positively correlated with the percentage of IL-10(+) CD19(+) B cells and serum IL-10 level. Serum IL-10 level and the percentage of IL-10(+) CD19(+) were negatively correlated with the percentages of CD38(+) CD19(+) , and CD86(+) CD19(+) B cells. These results suggest that CD86(+) CD19(+) , CD38(+) CD86(+) CD19(+) , CD95(+) CD19(+) , and especially CD38(+) CD19(+) and IL-10(+) CD19(+) cells may participate in the pathogenesis of HBV-MN.
authors.affiliation: Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, China.
authors.forename: Xintong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Changchun, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, China.
authors.forename: Huimao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Crew
authors.affiliation: Department of Medical Laboratory Sciences, Haramaya University, Dire Dawa, Ethiopia.
authors.forename: Desalegn Admassu
authors.initials: DA
authors.lastname: Ayana
authors.affiliation: Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, China.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, China.|Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000368:Aged; D018941:Antigens, CD19 / Q000378:metabolism; D060151:B-Lymphocytes, Regulatory / Q000166:cytology* / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000097:blood / Q000188:drug therapy / Q000276:immunology* / Q000821:virology*; D006509:Hepatitis B / Q000276:immunology; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D018941:Antigens, CD19; C508609:IL10 protein, human; D016753:Interleukin-10
keywords: HBV-MN; IL-10; breg cell; plasma cell