title: Decreased expression of long non-coding RNA MEG3 acts as a potential predictor biomarker in progression and poor prognosis of osteosarcoma.
abstract: BACKGROUND
  Long non-coding RNA MEG3 (lncRNA MEG3) has been showed to involve in a variety of cancers. However, the association between lncRNA MEG3 expression level and the prognosis of osteosarcoma is still unclear.
  
  
  METHODS
  The expression levels of lncRNA MEG3 in osteosarcoma tissues and adjacent non-tumor tissues were detected using quantitative real-time PCR (qRT-PCR). Differences in patient survival were determined using the Kaplan-Meier method and a log-rank test. A Cox proportional hazards regression analysis was used for univariate and multivariate analyses of prognostic values.
  
  
  RESULTS
  Our findings showed that expression of lncRNA MEG3 was clearly lower in osteosarcoma tissues compared with adjacent non-tumor tissues. The expression of lncRNA MEG3 was associated with clinical stage and distant metastasis (P<0.05). Kaplan-Meier analysis showed that patients with low lncRNA MEG3 expression had a shorter overall survival (log-rank test, P<0.05). Furthermore, multivariate analysis revealed that decreased expression of lncRNA MEG3, advanced clinical stage and distant metastasis were all independent predictors to overall survival of osteosarcoma patients.
  
  
  CONCLUSIONS
  Downregulation of lncRNA MEG3 was associated with poor overall survival of osteosarcoma. LncRNA MEG3 could be a useful biomarker for progression and prognosis of osteosarcoma.
authors.affiliation: Department of Orthopedics, Xinxiang Central Hospital Xinxiang 453000, China.
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Tian
authors.affiliation: Department of Orthopedics, Xinxiang Central Hospital Xinxiang 453000, China.
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, Xinxiang Central Hospital Xinxiang 453000, China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Xinxiang Central Hospital Xinxiang 453000, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002648:Child; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D012516:Osteosarcoma / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C498907:MEG3 non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: lncRNA MEG3; osteosarcoma; prognosis; progression
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