title: Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy.
abstract: Abscisic acid (ABA) is an important phytohormone controlling seed dormancy. AFPs (ABA INSENSITIVE FIVE BINDING PROTEINS) are reported to be negative regulators of the ABA signaling pathway. The involvement of AFPs in dormant vegetative organs remains poorly understood. Here, we isolated and characterized a novel AFP family member from Gladiolus dormant cormels, GhAFP-like, containing three conserved domains of the AFP family. Quantitative PCR analysis revealed that GhAFP-like was expressed in dormant organs and its expression was down-regulated along with corm storage. GhAFP-like was verified to be a nuclear-localized protein. Overexpressing GhAFP-like in Arabidopsis thaliana not only showed weaker seed dormancy with insensitivity to ABA, but also changed the expression of some ABA related genes. In addition, a primary root elongation assay showed GhAFP-like may involve in auxin signaling response. The results in this study indicate that GhAFP-like acts as a negative regulator in ABA signaling and is related to dormancy.
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Seng
authors.affiliation: Department of Biological Sciences, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Carina
authors.initials: C
authors.lastname: Carianopol
authors.affiliation: College of Biology, Fuyang Normal College, Fuyang, Anhui, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Sui
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China.
authors.forename: Huiru
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China. Electronic address: hejunna@cau.edu.cn.
authors.forename: Junna
authors.initials: J
authors.lastname: He
authors.affiliation: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China. Electronic address: ymfang@cau.edu.cn.
authors.forename: Mingfang
authors.initials: M
authors.lastname: Yi
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D003001:Cloning, Molecular; D018525:Germination / Q000502:physiology; D027621:Iridaceae / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D057445:Plant Dormancy / Q000502:physiology*; D035281:Plant Tubers
chemical_list: C409743:ABI5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D000040:Abscisic Acid; C106443:ABI2 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases
keywords: AFP family; AFP-like; Corm dormancy; Gladiolus hybridus