title: Measuring Exocytosis Rate Using Corrected Fluorescence Recovery After Photoconversion.
abstract: Exocytosis plays crucial roles in regulating the distribution and function of plasma membrane (PM) and extracellular matrix proteins. However, measuring the exocytosis rate of a specific protein by conventional methods is very difficult because of exocytosis-independent trafficking such as endocytosis, which also affects membrane protein distribution. Here, we describe a novel method, corrected fluorescence recovery after photoconversion, in which exocytosis-dependent and -independent trafficking events are measured simultaneously to accurately determine exocytosis rate. In this method, the protein-of-interest is tagged with Dendra2, a green-to-red photoconvertible fluorescent protein. Following the photoconversion of PM-localized Dendra2, both the recovery of the green signal and the changes in the photoconverted red signal are measured, and the rate of exocytosis is calculated from the changing rates of these two signals.
authors.affiliation: Center for Plant Cell Biology, Institute of Integrated Genome Biology, University of California, Riverside, CA, USA.|Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Luo
authors.affiliation: Center for Plant Cell Biology, Institute of Integrated Genome Biology, University of California, Riverside, CA, USA.|Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521, USA.|Current address: Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: An
authors.initials: A
authors.lastname: Yan
authors.affiliation: Center for Plant Cell Biology, Institute of Integrated Genome Biology, University of California, Riverside, CA, USA.|Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521, USA.
authors.forename: Zhenbiao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D005089:Exocytosis; D005453:Fluorescence; D008027:Light; D018613:Microscopy, Confocal
keywords: confocal microscopy; exocytosis; fluorescence recovery after photobleaching; membrane proteins; photoconversion; photoconvertible fluorescent protein
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