title: A Multi-Camera System for Bioluminescence Tomography in Preclinical Oncology Research.
abstract: Bioluminescent imaging (BLI) of cells expressing luciferase is a valuable noninvasive technique for investigating molecular events and tumor dynamics in the living animal. Current usage is often limited to planar imaging, but tomographic imaging can enhance the usefulness of this technique in quantitative biomedical studies by allowing accurate determination of tumor size and attribution of the emitted light to a specific organ or tissue. Bioluminescence tomography based on a single camera with source rotation or mirrors to provide additional views has previously been reported. We report here in vivo studies using a novel approach with multiple rotating cameras that, when combined with image reconstruction software, provides the desired representation of point source metastases and other small lesions. Comparison with MRI validated the ability to detect lung tumor colonization in mouse lung.
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. Matthew.Lewis@UTSouthwestern.edu.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Lewis
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. richer@lyle.smu.edu.|Department of Mechanical Engineering, Southern Methodist University, Dallas, TX 75275, USA. richer@lyle.smu.edu.
authors.forename: Edmond
authors.initials: E
authors.lastname: Richer
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. Nikolai.Slavine@UTSouthwestern.edu.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Slavine
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. Vikram.Kodibagkar@asu.edu.|School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA. Vikram.Kodibagkar@asu.edu.
authors.forename: Vikram D
authors.initials: VD
authors.lastname: Kodibagkar
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. Todd.Soesbe@UTSouthwestern.edu.|Advanced Imaging Research Center, UT Southwestern, Dallas, TX 75390, USA. Todd.Soesbe@UTSouthwestern.edu.
authors.forename: Todd C
authors.initials: TC
authors.lastname: Soesbe
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. antichino@earthlink.net.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Antich
authors.affiliation: Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA. Ralph.Mason@UTSouthwestern.edu.
authors.forename: Ralph P
authors.initials: RP
authors.lastname: Mason
keywords: MRI; bioluminescence; imaging systems; lung cancer; medical and biological imaging; tomography
references.citation: J Biophotonics. 2011 Nov;4(11-12):824-39
references.pmid: 21987294
references.citation: J Biomed Opt. 2008 Sep-Oct;13(5):054035
references.pmid: 19021415
references.citation: Acad Radiol. 2004 Sep;11(9):1029-38
references.pmid: 15350584
references.citation: Comput Math Methods Med. 2012;2012:494808
references.pmid: 22927887
references.citation: Biomed Opt Express. 2012 Nov 1;3(11):2794-808
references.pmid: 23162719
references.citation: Med Phys. 2006 Jan;33(1):61-8
references.pmid: 16485410
references.citation: Nature. 2008 Apr 3;452(7187):580-9
references.pmid: 18385732
references.citation: Rev Sci Instrum. 2012 Apr;83(4):043708
references.pmid: 22559540
references.citation: Methods Mol Biol. 2009;574:37-45
references.pmid: 19685298
references.citation: Indian J Exp Biol. 2007 Jan;45(1):58-63
references.pmid: 17249328
references.citation: Mol Imaging. 2004 Jan;3(1):9-23
references.pmid: 15142408
references.citation: Neoplasia. 2000 Jan-Apr;2(1-2):26-40
references.pmid: 10933066
references.citation: Front Biosci. 2008 Jan 01;13:1281-93
references.pmid: 17981629
references.citation: Med Phys. 2003 May;30(5):901-11
references.pmid: 12772999
references.citation: Opt Lett. 2006 Feb 1;31(3):365-7
references.pmid: 16480210
references.citation: Phys Med Biol. 2006 Apr 21;51(8):2045-53
references.pmid: 16585844
references.citation: Med Phys. 2004 Aug;31(8):2289-99
references.pmid: 15377096
references.citation: Cancer Res. 2004 Apr 15;64(8):2740-2
references.pmid: 15087388
references.citation: J Biomed Opt. 2006 Jul-Aug;11(4):041119
references.pmid: 16965147
references.citation: Mol Imaging. 2004 Apr;3(2):117-24
references.pmid: 15296676
references.citation: J Nucl Med. 2011 Aug;52(8):1268-75
references.pmid: 21810591
references.citation: Opt Express. 2009 Apr 27;17(9):7571-85
references.pmid: 19399136
references.citation: Curr Opin Chem Biol. 2010 Feb;14(1):80-9
references.pmid: 19962933
references.citation: Appl Opt. 2007 May 10;46(14):2778-84
references.pmid: 17446927
references.citation: Phys Med Biol. 2008 Jul 21;53(14 ):3921-42
references.pmid: 18591735
references.citation: Appl Opt. 2008 Feb 20;47(6):799-806
references.pmid: 18288229
references.citation: Opt Express. 2006 Aug 21;14(17):7852-71
references.pmid: 19529153
references.citation: Opt Express. 2008 Sep 29;16(20):15640-54
references.pmid: 18825203
references.citation: Phys Med Biol. 2007 Aug 7;52(15):4497-512
references.pmid: 17634646
references.citation: J Pathol. 2010 Feb;220(3):317-27
references.pmid: 19967724
references.citation: Opt Express. 2009 Nov 23;17(24):21925-34
references.pmid: 19997437
references.citation: Opt Lett. 2001 Jun 15;26(12):893-5
references.pmid: 18040483
references.citation: Med Phys. 2011 Nov;38(11):5933-44
references.pmid: 22047358
references.citation: Med Phys. 2008 Nov;35(11):4863-71
references.pmid: 19070220
references.citation: J Biomed Opt. 2004 May-Jun;9(3):578-86
references.pmid: 15189096
references.citation: Opt Express. 2010 Mar 29;18(7):6477-91
references.pmid: 20389671
references.citation: Opt Express. 2006 Sep 4;14(18):8211-23
references.pmid: 19529195
references.citation: Nat Photonics. 2007;1(9):526-530
references.pmid: 18974848
references.citation: J Biomed Opt. 2001 Oct;6(4):432-40
references.pmid: 11728202
references.citation: Opt Express. 2009 May 11;17(10):8062-80
references.pmid: 19434138
references.citation: PLoS One. 2010 Aug 06;5(8):e12024
references.pmid: 20700459
references.citation: Annu Rev Biomed Eng. 2007;9:321-49
references.pmid: 17461729
references.citation: ACS Chem Biol. 2012 Nov 16;7(11):1848-57
references.pmid: 22894855
references.citation: Opt Express. 2005 Sep 5;13(18):6756-71
references.pmid: 19498692
references.citation: Appl Opt. 2007 May 1;46(13):2540-7
references.pmid: 17429468
references.citation: J Surg Res. 2004 Aug;120(2):249-55
references.pmid: 15234220
references.citation: Appl Opt. 2012 Jul 1;51(19):4501-12
references.pmid: 22772124
references.citation: Opt Express. 2008 Feb 4;16(3):1719-32
references.pmid: 18542251
references.citation: Phys Med Biol. 2005 Dec 7;50(23):5421-41
references.pmid: 16306643
references.citation: J Biomed Opt. 2007 Mar-Apr;12(2):024007
references.pmid: 17477722
references.citation: Phys Med Biol. 2005 Sep 7;50(17):4225-41
references.pmid: 16177541
references.citation: PLoS One. 2012;7(9):e46106
references.pmid: 23029403
references.citation: Opt Express. 2004 Aug 23;12(17):3996-4000
references.pmid: 19483937
references.citation: J Comput Math. 2008;26(3):324-335
references.pmid: 20617105