title: Determination of Chondroitin Sulfate Content in Raw Materials and Dietary Supplements by High-Performance Liquid Chromatography with UV Detection After Enzymatic Hydrolysis: Single-Laboratory Validation First Action 2015.11.
abstract: A previously validated method for determination of chondroitin sulfate in raw materials and dietary supplements was submitted to the AOAC Expert Review Panel (ERP) for Stakeholder Panel on Dietary Supplements Set 1 Ingredients (Anthocyanins, Chondroitin, and PDE5 Inhibitors) for consideration of First Action Official Methods(SM) status. The ERP evaluated the single-laboratory validation results against AOAC Standard Method Performance Requirements 2014.009. With recoveries of 100.8-101.6% in raw materials and 105.4-105.8% in finished products and precision of 0.25-1.8% RSDr within-day and 1.6-4.72% RSDr overall, the ERP adopted the method for First Action Official Methods status and provided recommendations for achieving Final Action status.
authors.affiliation: Brunelle Biotech Consulting, 14104 194th Ave NE, Woodinville, WA 98077, USA.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Brunelle
mesh_terms: D002809:Chondroitin Sulfates / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis; D007753:Laboratories; D014466:Ultraviolet Rays
chemical_list: D004798:Enzymes; D002809:Chondroitin Sulfates
references.citation: J AOAC Int. 2007 May-Jun;90(3):659-69
references.pmid: 17580617