title: Role of dietary flavonoids in amelioration of sugar induced cataractogenesis.
abstract: Sugar induced cataractogenesis and visual impairment is more prominent ophthalmic problem in humans suffering from diabetes. Flavonoids have been identified as one of the therapeutically important class of phytochemicals possessing myriad of biological activities. Analyzing the anti-cataract effects of flavonoids from natural sources is an important aspect owing to their bioavailability in variety of dietary sources. In the present study a panel of ten dietary flavonoids like 3, 6-dihydroxy flavone, 3, 7-dihydroxy flavone, chrysin, 3-hydroxy-7-methoxy flavone, apigenin, genistein, baicalein, galangin, Biochanin-A, and diosmin were evaluated for their anti-cataract effects in sugar induced lens model studies. Series of parameters like role of flavonoids in glycation induced lens opacity, protein aggregation measurements, carbonyl group formation: a biochemical marker of glycation reaction, non-tryptophan fluorescence: a marker of formation of advanced glycation end products (AGEs) and assessment of (experimental and in silico) aldose reductase inhibition: a key enzyme of polyol pathway involved in cataractogenesis. The results of the study clearly demonstrated the impressive anti-cataract activity of chrysin followed by significant activity by apigenin, baicalein and genistein. The results of the present study may find applications in formulation of functional foods and neutraceuticals for the management of diabetic cataract.
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Kapil K
authors.initials: KK
authors.lastname: Patil
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Rohan J
authors.initials: RJ
authors.lastname: Meshram
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India; Department of Biochemistry, Savitribai Phule Pune University, Pune-7, MS, India.
authors.forename: Nagesh A
authors.initials: NA
authors.lastname: Dhole
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India. Electronic address: rngacche@rediffmail.com.
authors.forename: Rajesh N
authors.initials: RN
authors.lastname: Gacche
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors / Q000737:chemistry; D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000188:drug therapy*; D003198:Computer Simulation; D004040:Dietary Carbohydrates; D005136:Eye Proteins / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose; D006031:Glycosylation; D006041:Goats; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D062105:Molecular Docking Simulation; D064209:Phytochemicals / Q000737:chemistry / Q000494:pharmacology*; D066329:Protein Aggregates; D050050:Protein Carbonylation; D013329:Structure-Activity Relationship
chemical_list: D004040:Dietary Carbohydrates; D005136:Eye Proteins; D005419:Flavonoids; D064209:Phytochemicals; D066329:Protein Aggregates; D000449:Aldehyde Reductase; D005947:Glucose
keywords: Cataractogenesis; Eye lens; Flavonoids; Glycation; Protein aggregation