title: Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head.
abstract: Autologous implantation of bone mesenchymal stem cells (BMSCs) has achieved promising clinical efficacy for the treatment of early-stage osteonecrosis of the femoral head (ONFH). However, the underlying mechanisms are not completely elucidated. Here, we investigated the effect of BMSCs on the early ONFH in vitro and in vivo. In co-cultured system, primary BMSCs enhanced the activity and inhibited the apoptosis of primary OB. The concentrations of VEGF and BMP-2 in the co-cultured medium were significantly higher than those without co-culture. Importantly, BMSCs implantation increased OB, capillaries and VEGF and BMP-2 expressions of the necrotic areas of femoral head in the ONFH rabbits. In conclusion, our results indicated that BMSCs treated the early ONFH possibly through increasing OB and capillaries, as well as VEGF and BMP-2 expression in the femoral head. These results provided possible mechanisms for the treatment of early-stage ONFH with BMSCs transplantation.
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University Xi'an 710004, Shaanxi Province, China.
authors.forename: Huanjin
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Orthopedics, Xi'an Honghui Hospital Xi'an 710054, Shaanxi Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.affiliation: Department of Clinical Hematology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University Xi'an 710004, Shaanxi Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University Xi'an 710004, Shaanxi Province, China.
authors.forename: Weizhuo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Clinical Oncology, First Affiliated Hospital, College of Medicine of Xi'an Jiaotong University Xi'an 710061, Shaanxi Province, China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Division of Endocrinology, Gerontology and Metabolism, School of Medicine, Stanford University Stanford, CA, USA.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Clinical Hematology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University Xi'an 710004, Shaanxi Province, China.
authors.forename: Fuling
authors.initials: F
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis; D060833:Cellular Microenvironment; D018920:Coculture Techniques; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005271:Femur Head Necrosis / Q000378:metabolism / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D010006:Osteoblasts / Q000378:metabolism*; D011817:Rabbits; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D055396:Bone Morphogenetic Protein 2; D042461:Vascular Endothelial Growth Factor A
keywords: BMP-2; BMSCs; OB; ONFH; VEGF
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