title: Use of an Electrochemical Split Cell Technique to Evaluate the Influence of Shewanella oneidensis Activities on Corrosion of Carbon Steel.
abstract: Microbially induced corrosion (MIC) is a complex problem that affects various industries. Several techniques have been developed to monitor corrosion and elucidate corrosion mechanisms, including microbiological processes that induce metal deterioration. We used zero resistance ammetry (ZRA) in a split chamber configuration to evaluate the effects of the facultatively anaerobic Fe(III) reducing bacterium Shewanella oneidensis MR-1 on the corrosion of UNS G10180 carbon steel. We show that activities of S. oneidensis inhibit corrosion of steel with which that organism has direct contact. However, when a carbon steel coupon in contact with S. oneidensis was electrically connected to a second coupon that was free of biofilm (in separate chambers of the split chamber assembly), ZRA-based measurements indicated that current moved from the S. oneidensis-containing chamber to the cell-free chamber. This electron transfer enhanced the O2 reduction reaction on the coupon deployed in the cell free chamber, and consequently, enhanced oxidation and corrosion of that electrode. Our results illustrate a novel mechanism for MIC in cases where metal surfaces are heterogeneously covered by biofilms.
authors.affiliation: Department of Biology, The University of Akron, Akron, OH 44325, United States of America.|Integrated Bioscience Program, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: Robert Bertram
authors.initials: RB
authors.lastname: Miller
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: Anwar
authors.initials: A
authors.lastname: Sadek
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Rodriguez
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: Mariano
authors.initials: M
authors.lastname: Iannuzzi
authors.affiliation: Department of Geosciences, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: Carla
authors.initials: C
authors.lastname: Giai
authors.affiliation: Department of Biology, The University of Akron, Akron, OH 44325, United States of America.|Integrated Bioscience Program, The University of Akron, Akron, OH 44325, United States of America.|Department of Geosciences, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: John M
authors.initials: JM
authors.lastname: Senko
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, United States of America.
authors.forename: Chelsea N
authors.initials: CN
authors.lastname: Monty
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism; D003343:Corrosion; D004560:Electricity; D055664:Electrochemical Techniques; D004579:Electron Transport; D005290:Ferric Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D020592:Shewanella / Q000737:chemistry* / Q000378:metabolism; D013232:Steel / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D013232:Steel; D002244:Carbon; D010100:Oxygen
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