title: Effect of Shenfu injection on immune function of mice bearing Lewis lung sarcoma with chemotherapy.
abstract: Chemotherapy damages immune system and has a lot of side effects. In this study, we investigate the function of Shenfu injection to reduce these unfavorable effects induced by chemotherapy on mice bearing Lewis lung sarcoma. Mice inoculated with Lewis lung sarcoma cells were divided into five groups: Lewis lung sarcoma control group, cyclophosphamide (CTX) group, and Shenfu injection (high, moderate, and low dose) + CTX group. After a 14-day treatment, the counting of peripheral blood cells, CD3+, CD4+, and CD8+ T lymphocytes were done, immunoglobulin (Ig) was measured, coefficients of spleen and thymus were calculated, and spleen T cell proliferation was evaluated in vitro. The CD4+/CD8+ and CD3+ T cells in high- and moderate-dose Shenfu groups were more than the CTX group (p < 0.05); spleen T cell proliferation of mice in high-dose Shenfu + CTX group is more prominent than the CTX group (p < 0.05); coefficients of spleen and thymus, WBC, and platelet (PLT) counting of mice in the CTX group were lower than control and high and moderate dose Shenfu + CTX groups. The level of serous IgG and IgM of all test groups shows no significant difference. Shenfu injection can improve cellular immune function and reduce myolosuppression of mice delivered with chemotherapy.
authors.affiliation: Department of Radiation Oncology, Sichuan Cancer Hospital and Institute, Chengdu, Sichuan, 610041, China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Thoracic Surgery, Sichuan Cancer Hospital and Institute, No.55 Section 4 of Renmin South Rd. Wuhou, Chengdu, Sichuan, 610041, People's Republic of China. wangkangning123@gmail.com.
authors.forename: Kangning
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Sichuan Cancer Hospital and Institute, No.55 Section 4 of Renmin South Rd. Wuhou, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, Sichuan Cancer Hospital and Institute, Chengdu, Sichuan, 610041, China.
authors.forename: Jinyi
authors.initials: J
authors.lastname: Lang
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000627:therapeutic use / Q000633:toxicity*; D001855:Bone Marrow Diseases / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000276:immunology; D003520:Cyclophosphamide / Q000627:therapeutic use / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D007107:Immune System / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D018906:Antineoplastic Agents, Alkylating; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C492793:Shen-Fu; D003520:Cyclophosphamide
keywords: Chemotherapy; Immune function; Lewis lung sarcoma; Mice; Shenfu injection
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