title: [COLD-ADAPTED A/KRASNODAR/101/35/59 (H2N2) STRAIN--A PROMISING STRAIN-DONOR OF ATTENUATION FOR PROCURATION OF LIVE INFLUENZA VACCINES].
abstract: OBJECTIVE
  Study of ts, ca, att phenotype, immunogenicity and protective effectiveness of reassortants obtained by a way of recombination of a new influenza cold-adapted (ca) strain donor of attenuation A/Krasnodar/101/35/59 (H2N2) and virulent strain of influenza virus.
  
  
  METHODS
  Viruses were used: ca strain A/Krasnodar/101/35.59 (H2N2), virulent strains: A/Kumamoto/102/02 (H3N2) and A/Bern/07/95. For determination of ts and ca phenotype, titration of viruses in chicken embryos was carried out simultaneously at optimal, decreased and increased temperature. Protective effect of immunization was evaluated during intranasal infection of mice with a virulent strain of influenza virus.
  
  
  RESULTS
  All the obtained reassortants possessed 6 internal genes from strain-donor of attenuation and 2 genes, coding HA and NA-proteins from virulent strains. Ca reassortants were characterized by ts and ca phenotype, had antigenic specificity and good immunogenicity, had high protective effectiveness.
  
  
  CONCLUSIONS
  The data obtained indicate on the perspectiveness of ca strain A/Krasnodar/101/35/59 (H2N2)as a donor of attenuation for live influenza vaccines.
authors.forename: S G
authors.initials: SG
authors.lastname: Markushin
authors.forename: T M
authors.initials: TM
authors.lastname: Tsfasman
authors.forename: A V
authors.initials: AV
authors.lastname: Terekhov
authors.forename: K V
authors.initials: KV
authors.lastname: Lisovskaya
authors.forename: I I
authors.initials: II
authors.lastname: Akopova
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003080:Cold Temperature; D006801:Humans; D007114:Immunization; D053121:Influenza A Virus, H2N2 Subtype / Q000276:immunology / Q000472:pathogenicity; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology* / Q000627:therapeutic use; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D051379:Mice; D014613:Vaccines, Attenuated / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated