title: Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis.
abstract: BACKGROUND
  Technique failure in peritoneal dialysis (PD) due to fibrosis and angiogenesis is complicated by peritonitis. Staphylococcus aureus infection is one of the most common causes of peritonitis in PD. The heparan sulfate proteoglycan, syndecan-1 (CD138), was reported to regulate fibrosis, angiogenesis, inflammation and S. aureus infection. The objectives of this study were to examine the effects of syndecan-1 on S. aureus infection and histopathology in a PD model.
  
  
  RESULTS
  Syndecan-1(-/-) and wild type mice were dialyzed for 4 weeks and infected intraperitoneally with S. aureus. Tissues were collected after 4 h for histomorphometric analysis. Intravital microscopy was used to observe leukocyte recruitment and to quantify syndecan-1 in the parietal peritoneum microcirculation. The dialyzed syndecan-1(-/-) mice were more susceptible to S. aureus infection than undialyzed syndecan-1(-/-) controls and wild type animals. However, peritoneal fibrosis and neovascularization due to PD did not differ between syndecan-1(-/-) and wild type mice. Intravital microscopy showed that in S. aureus infection, syndecan-1 was removed from the subendothelial layer of peritoneal venules but syndecan-1 deficiency did not affect leukocyte recruitment.
  
  
  CONCLUSIONS
  This study indicates that, while syndecan-1 is important for providing a barrier to acute S. aureus infection in PD, it does not affect peritoneal fibrosis and angiogenesis.
authors.affiliation: Thrombosis and Atherosclerosis Research Institute and the Department of Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: Paulina M
authors.initials: PM
authors.lastname: Kowalewska
authors.affiliation: Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Diseases Research, McMaster University, Hamilton, ON, Canada.
authors.forename: Uyen T
authors.initials: UT
authors.lastname: Nguyen
authors.affiliation: Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Diseases Research, McMaster University, Hamilton, ON, Canada.
authors.forename: Lori L
authors.initials: LL
authors.lastname: Burrows
authors.affiliation: Thrombosis and Atherosclerosis Research Institute and the Department of Medicine, McMaster University, Hamilton, ON, Canada. afoxrob@mcmaster.ca.|David Braley Cardiac, Vascular and Stroke Research Institute, C5-106, 237 Barton Street East, Hamilton, ON, L8L 2X2, Canada. afoxrob@mcmaster.ca.
authors.forename: Alison E
authors.initials: AE
authors.lastname: Fox-Robichaud
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D056627:Peritoneal Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D013203:Staphylococcal Infections / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013211:Staphylococcus aureus / Q000378:metabolism*; D053668:Syndecan-1 / Q000172:deficiency*
chemical_list: C508242:Sdc1 protein, mouse; D053668:Syndecan-1
references.citation: Microcirculation. 2011 Nov;18(8):613-22
references.pmid: 21790840
references.citation: Perit Dial Int. 2016 Jan-Feb;36(1):7-15
references.pmid: 26475840
references.citation: J Immunol. 2002 May 1;168(9):4650-8
references.pmid: 11971014
references.citation: Adv Perit Dial. 2011;27:38-42
references.pmid: 22073826
references.citation: Mol Biol Cell. 2005 Aug;16(8):3678-91
references.pmid: 15930135
references.citation: Perit Dial Int. 2007 Jul-Aug;27(4):375-90
references.pmid: 17602143
references.citation: Hypertension. 2010 Feb;55(2):249-56
references.pmid: 20048198
references.citation: Adv Perit Dial. 2009;25:80-4
references.pmid: 19886323
references.citation: Microcirculation. 2004 Dec;11(8):679-87
references.pmid: 15726835
references.citation: Perit Dial Int. 2010 May-Jun;30(3):284-93
references.pmid: 20150585
references.citation: Curr Eye Res. 2005 Jun;30(6):417-22
references.pmid: 16020273
references.citation: J Anat. 1991 Aug;177:41-6
references.pmid: 1769897
references.citation: Circulation. 2007 Jan 30;115(4):475-82
references.pmid: 17242279
references.citation: J Biol Chem. 2007 Jan 5;282(1):159-67
references.pmid: 17098735
references.citation: J Immunol. 2009 Apr 15;182(8):4985-93
references.pmid: 19342678
references.citation: J Biol Chem. 2011 Oct 14;286(41):35891-8
references.pmid: 21878647
references.citation: Shock. 2008 Dec;30(6):623-7
references.pmid: 18497712
references.citation: Int J Mol Sci. 2013;14(5):10369-82
references.pmid: 23685870
references.citation: J Am Soc Nephrol. 2005 Feb;16(2):425-36
references.pmid: 15590759
references.citation: Perit Dial Int. 2007 Jun;27 Suppl 2:S110-5
references.pmid: 17556288
references.citation: J Invest Dermatol. 2008 Jan;128(1):26-34
references.pmid: 17625591
references.citation: Infect Immun. 2012 May;80(5):1670-80
references.pmid: 22354024
references.citation: Nephrol Dial Transplant. 2004 Oct;19(10):2584-91
references.pmid: 15304559
references.citation: J Biol Chem. 2012 Oct 12;287(42):34927-35
references.pmid: 22936802
references.citation: Expert Rev Anti Infect Ther. 2015 May;13(5):605-13
references.pmid: 25746414
references.citation: Int J Gynecol Cancer. 2010 Jul;20(5):751-6
references.pmid: 20973264
references.citation: Cytokine. 2003 Mar 7;21(5):224-33
references.pmid: 12824007
references.citation: J Mater Sci Mater Med. 1999 Oct-Nov;10(10/11):579-88
references.pmid: 15347970
references.citation: Kidney Int. 2007 Nov;72(10):1204-15
references.pmid: 17805240
references.citation: Infect Immun. 2005 Dec;73(12):7914-21
references.pmid: 16299282
references.citation: Immunity. 2001 Jun;14(6):705-14
references.pmid: 11420041
references.citation: J Am Soc Nephrol. 2002 Feb;13(2):470-9
references.pmid: 11805177
references.citation: Mol Biol Cell. 1995 May;6(5):559-76
references.pmid: 7545031
references.citation: Kidney Int Suppl. 2003 Dec;(88):S43-9
references.pmid: 14870877
references.citation: J Cell Sci. 2002 Dec 1;115(Pt 23):4517-31
references.pmid: 12414997
references.citation: J Clin Invest. 2008 Jan;118(1):229-38
references.pmid: 18064305
references.citation: Kidney Int. 1998 Dec;54(6):2207-17
references.pmid: 9853287
references.citation: Br J Haematol. 1997 Nov;99(2):368-71
references.pmid: 9375756
references.citation: Invest Ophthalmol Vis Sci. 2002 Apr;43(4):1135-41
references.pmid: 11923257
references.citation: Nature. 2001 May 3;411(6833):98-102
references.pmid: 11333985
references.citation: J Biol Chem. 2011 Feb 4;286(5):3288-97
references.pmid: 21127056
references.citation: Perit Dial Int. 2010 Mar-Apr;30(2):170-7
references.pmid: 20124193
references.citation: J Biol Chem. 2012 Jan 6;287(2):1478-88
references.pmid: 22102278
references.citation: Microvasc Res. 2013 May;87:83-91
references.pmid: 23428342
references.citation: Cell. 2002 Nov 27;111(5):635-46
references.pmid: 12464176
references.citation: J Immunol. 2013 Nov 1;191(9):4551-61
references.pmid: 24078687
references.citation: FASEB J. 2015 Feb;29(2):711-23
references.pmid: 25416549
references.citation: Arterioscler Thromb Vasc Biol. 2015 Feb;35(2):332-40
references.pmid: 25550207
references.citation: Nephrol Dial Transplant. 2011 Sep;26(9):2995-3005
references.pmid: 21324976
references.citation: PLoS One. 2014;9(9):e104537
references.pmid: 25184228
references.citation: Br J Pharmacol. 2010 Aug;160(7):1577-9
references.pmid: 20649561
references.citation: Perit Dial Int. 2003 Sep-Oct;23(5):456-9
references.pmid: 14604197
references.citation: J Biol Chem. 2004 Jan 2;279(1):251-8
references.pmid: 14573623