title: Simultaneous determination of benznidazole and itraconazole using spectrophotometry applied to the analysis of mixture: A tool for quality control in the development of formulations.
abstract: The aim of this work was the development of an analytical procedure using spectrophotometry for simultaneous determination of benznidazole (BNZ) and itraconazole (ITZ) in a medicine used for the treatment of Chagas disease. In order to achieve this goal, the analysis of mixtures was performed applying the Lambert-Beer law through the absorbances of BNZ and ITZ in the wavelengths 259 and 321 nm, respectively. Diverse tests were carried out for development and validation of the method, which proved to be selective, robust, linear, and precise. The lower limits of detection and quantification demonstrate its sensitivity to quantify small amounts of analytes, enabling its application for various analytical purposes, such as dissolution test and routine assays. In short, the quantification of BNZ and ITZ by analysis of mixtures had shown to be efficient and cost-effective alternative for determination of these drugs in a pharmaceutical dosage form.
authors.affiliation: Laboratory of Food, Drugs and Cosmetics, University of Brasília (UnB), 70910-900, Brasília-DF, Brazil.
authors.forename: Ludmila A G
authors.initials: LA
authors.lastname: Pinho
authors.affiliation: Faculty of Ceilândia, University of Brasília (UnB), 72220-900, Brasília-DF, Brazil.
authors.forename: Lívia C L
authors.initials: LC
authors.lastname: Sá-Barreto
authors.affiliation: Faculty of Health Sciences, University of Brasília (UnB), 70910-900, Brasília-DF, Brazil.
authors.forename: Carlos M C
authors.initials: CM
authors.lastname: Infante
authors.affiliation: Laboratory of Food, Drugs and Cosmetics, University of Brasília (UnB), 70910-900, Brasília-DF, Brazil. Electronic address: marciliofarm@hotmail.com.
authors.forename: Marcílio S S
authors.initials: MS
authors.lastname: Cunha-Filho
mesh_terms: D000935:Antifungal Agents / Q000032:analysis*; D014355:Chagas Disease / Q000188:drug therapy; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004338:Drug Combinations; D006801:Humans; D017964:Itraconazole / Q000032:analysis*; D057230:Limit of Detection; D009593:Nitroimidazoles / Q000032:analysis*; D011786:Quality Control; D013053:Spectrophotometry / Q000379:methods*; D014344:Trypanocidal Agents / Q000032:analysis*
chemical_list: D000935:Antifungal Agents; D004338:Drug Combinations; D009593:Nitroimidazoles; D014344:Trypanocidal Agents; D017964:Itraconazole; C009999:benzonidazole
keywords: Benznidazole; Chagas disease; Itraconazole; Spectrophotometric analysis of mixtures