title: Downregulation of let-7e-5p contributes to endothelial progenitor cell dysfunction in deep vein thrombosis via targeting FASLG.
abstract: This study aimed to evaluate the role of let-7e-5p in endothelial progenitor cells (EPCs) function and explore its therapeutic potential for deep vein thrombosis (DVT). We performed miRNAs screening and found that let-7e-5p was downregulated in DVT patients compared to control subjects. By using let-7e-5p agomir and antagomir, we demonstrated that let-7e-5p increased the migration and tube formation of human and rat EPCs. Based on bioinformatics, luciferase reporter assay and gene expression analysis, we identified Fas ligand (FASLG) as the target of let-7e-5p, and FASLG knockdown increased the migration and tube formation of EPCs. Furthermore, EPCs overexpressing let-7e-5p exhibited enhanced ability of homing and thrombus revascularization inrat model of venous thrombosis. In conclusion, let-7e-5p regulates the function of EPCs and is a potential therapeutic target in DVT treatment.
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Lingshang
authors.initials: L
authors.lastname: Kong
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Wendong
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhuang
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China. Electronic address: 563677272@qq.com.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China. Electronic address: ning2005@139.com.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D015536:Down-Regulation; D066026:Endothelial Progenitor Cells / Q000378:metabolism / Q000473:pathology*; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D020246:Venous Thrombosis / Q000235:genetics* / Q000473:pathology
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human
keywords: Deep vein thrombosis; Endothelial progenitor cells; Fas ligand; Let-7e-5p