title: [Effects of inward rectifier potassium channel blockers on EPCs function].
abstract: OBJECTIVE
  To investigate the effects of inward rectifier potassium channel blockers (BaCl2, CsCl) on the functions of endothelial progenitor cells (EPCs).
  
  
  METHODS
  Density gradient centrifugation-isolated rat hone marrow mononuclear cells were cultured in vitro. EPCs were harvested and seeded on six culture dish when cells grew to 3-5 passages. Before testing the EPCs were synchronized with M199, which contain 2% fetal calf serum. In the end, EPCs were treated with different intervention. The experiment mainly included two parts: (1) BaCl2 (100 micromol/L) and free BaC2 of Tyrodes solution; (2) CsCl (1 mmol/L) and control. Cell pretreated with blockers above mentioned for 12 h, then the gene expression of stromal cell-derived factor-1 (SDF-1), epoprotenol (PGI2) were assessed, beyond that the ability of adhesion, migration were assayed with different tests. In addition, the medium was collected when EPCs were treated for 3 days. The levels of SDF-1 were measured by sandwich enzyme-linked immunosorbent assay (ELISA). Going even further, EPCs were treated with the signal pathway blockers in advance, after repeat the above steps, in order to analyze the change of SDF-1 and then discuss its mechanism.
  
  
  RESULTS
  Compared with control group, BaCl2, CsCl could increase EPC adhesion and migration to same extent. Moreover, the gene expression of SDF-1, PGI2 was significantly up-regulated and the production of SDF-1 increased evidently. Furthermore, the mechanism of SDF-1 secretion increasing mainly was associated with eNOS signaling pathways.
  
  
  CONCLUSIONS
  Ba2+ and Cs+ play important roles in increasing EPCs functions, such as adhesion, migration and secretion.
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Li
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Cui
authors.forename: Ning-ning
authors.initials: NN
authors.lastname: Hou
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
mesh_terms: D000818:Animals; D017609:Barium Compounds / Q000494:pharmacology; D002478:Cells, Cultured; D002586:Cesium / Q000494:pharmacology; D054377:Chemokine CXCL12 / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D024661:Potassium Channels, Inwardly Rectifying / Q000037:antagonists & inhibitors* / Q000502:physiology; D051381:Rats; D013234:Stem Cells / Q000166:cytology*
chemical_list: D017609:Barium Compounds; C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; D002712:Chlorides; D024661:Potassium Channels, Inwardly Rectifying; C024986:barium chloride; D002586:Cesium; C028019:cesium chloride