title: Anti-tumor effect of ultrasound-induced Nordy-loaded microbubbles destruction.
abstract: Synthesized dl-Nordihydroguaiaretic acid (dl-NGDA or "Nordy") can inhibit the growth of malignant human tumors, especially the tumor angiogenesis. However, its liposoluble nature limits its in vivo efficacy in the hydrosoluble circulation of human.
  
  
  
  We tried to use the ultrasonic microbubble as the carrier and the ultrasound-induced destruction for the targeted release of Nordy and evaluate its in vitro and in vivo anti-tumor effect.
  
  
  
  Nordy-loaded lipid microbubbles were prepared by mechanical vibration. Effects of ultrasound-induced Nordy-loaded microbubbles destruction on proliferation of human umbilical vein endothelial cells (HUVECs), tumor derived endothelial cells (Td-ECs), and rabbit transplanted VX2 tumor models were evaluated.
  
  
  
  The ultrasound-induced Nordy-loaded microbubbles destruction inhibited the proliferations of HUVECs and Td-ECs in vitro, and inhibited the tumor growth and the microvasculature in vivo. Its efficacy was higher than those of Nordy used only and Nordy with ultrasound exposure.
  
  
  
  Ultrasonic microbubbles can be used as the carrier of Nordy and achieve its targeted release with improved anti-tumor efficacy in the condition of ultrasound-induced microbubbles destruction.
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Xing
authors.initials: X
authors.lastname: Hua
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ding
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: a Third Military Medical University, Southwest Hospital , Chongqing , China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Chen
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage* / Q000627:therapeutic use; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009637:Masoprocol / Q000008:administration & dosage* / Q000627:therapeutic use; D045423:Microbubbles; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000188:drug therapy; D015612:Phonophoresis / Q000379:methods*; D011817:Rabbits; D000069453:Ultrasonic Waves
chemical_list: D020533:Angiogenesis Inhibitors; D009637:Masoprocol
keywords: Anti-tumor effect; Nordy; drug-targeted delivery; microbubbles; ultrasound