title: Differential loss of natural killer cell activity in patients with acute myocardial infarction and stable angina pectoris.
abstract: BACKGROUND
  To evaluate the activity of natural killer cells through their inhibitory and activating receptors and quantity in peripheral blood mononuclear cells extracted from patients with acute myocardial infarction, stable angina pectoris and the controls.
  
  
  METHODS
  100 patients with myocardial infarction, 100 with stable angina, and 20 healthy volunteers were recruited into the study. 20 randomly chosen people per group were examined for the whole human genome microarray analysis to detect the gene expressions of all 40 inhibitory and activating natural killer cell receptors. Flow cytometry analysis was applied to all 200 patients to measure the quantity of natural killer cells.
  
  
  RESULTS
  In myocardial infarction group, the mRNA expressions of six inhibitory receptors KIR2DL2, KIR3DL3, CD94, NKG2A, KLRB1, KLRG1, and eight activating receptors KIR2DS3, KIR2DS5, NKp30, NTB-A, CRACC, CD2, CD7 and CD96 were significantly down-regulated (P<0.05) compared with both angina patients and the controls. There was no statistical difference in receptor expressions between angina patients and control group. The quantity of natural killer cells was significantly decreased in both infarction and angina patients compared with normal range (P<0.001).
  
  
  CONCLUSIONS
  The significant mRNAs down-regulation of several receptors in myocardial infarction group and reduction in the quantity of natural killer cells in both myocardial infarction and angina patients showed a quantitative loss and dysfunction of natural killer cells in myocardial infarction patients.
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Siwan
authors.initials: S
authors.lastname: Wen
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Qianglin
authors.initials: Q
authors.lastname: Duan
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Yongyan
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine 389 Xincun Rd, Putuo District, Shanghai 200065, China.
authors.forename: Lemin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D060050:Angina, Stable / Q000276:immunology / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000276:immunology / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D059467:Transcriptome
keywords: Myocardial infarction; gene expression; natural killer cell activating receptors; natural killer cell inhibitory receptors; natural killer cells; stable angina pectoris
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