title: Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration.
abstract: Analysis of thousands of Δmsh-2 octads using our fluorescent recombination system indicates that, as in other filamentous fungi, symmetric heteroduplex is common in the his-3 region of Neurospora crassa. Symmetric heteroduplex arises from Holliday junction migration, and we suggest this mechanism explains the high frequency of His+ spores in heteroallelic crosses in which recombination is initiated cis to the his-3 allele further from the initiator, cog+. In contrast, when recombination is initiated cis to the his-3 allele closer to cog+, His+ spores are mainly a result of synthesis-dependent strand annealing, yielding asymmetric heteroduplex. Loss of Msh-2 function increases measures of allelic recombination in both his-3 and the fluorescent marker gene, indicating that mismatches in asymmetric heteroduplex, as in Saccharomyces cerevisiae, tend to be repaired in the direction of restoration. Furthermore, the presence of substantial numbers of conversion octads in crosses lacking Msh-2 function suggests that the disjunction pathway described in S. cerevisiae is also active in Neurospora, adding to evidence for a universal model for meiotic recombination.
authors.affiliation: School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia.
authors.forename: Patricia Jane
authors.initials: PJ
authors.lastname: Yeadon
authors.affiliation: School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia.
authors.forename: Frederick James
authors.initials: FJ
authors.lastname: Bowring
authors.affiliation: School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia.
authors.forename: David E A
authors.initials: DE
authors.lastname: Catcheside
mesh_terms: D000483:Alleles; D045643:DNA Repair Enzymes / Q000235:genetics / Q000378:metabolism; D045566:DNA, Cruciform / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D059765:Homologous Recombination; D008540:Meiosis; D009492:Neurospora crassa / Q000235:genetics*; D010641:Phenotype
chemical_list: D045566:DNA, Cruciform; D005656:Fungal Proteins; D045643:DNA Repair Enzymes
references.citation: Proc Natl Acad Sci U S A. 1981 Dec;78(12):7648-51
references.pmid: 6950408
references.citation: Genetics. 2008 Mar;178(3):1141-5
references.pmid: 18385109
references.citation: Genetics. 2013 Jun;194(2):327-34
references.pmid: 23733849
references.citation: Microbiol Mol Biol Rev. 1999 Jun;63(2):349-404
references.pmid: 10357855
references.citation: Annu Rev Genet. 1990;24:579-613
references.pmid: 2150906
references.citation: Aust J Biol Sci. 1970 Dec;23(6):1229-40
references.pmid: 5496221
references.citation: Cell. 2001 Nov 16;107(4):537-48
references.pmid: 11719193
references.citation: Proc Natl Acad Sci U S A. 1955 Apr 15;41(4):215-20
references.pmid: 16589648
references.citation: Genetics. 2010 Oct;186(2):515-36
references.pmid: 20679514
references.citation: Nucleic Acids Res. 2003 May 15;31(10):2576-85
references.pmid: 12736307
references.citation: Genetics. 2002 Aug;161(4):1483-95
references.pmid: 12196394
references.citation: Cell. 2001 Dec 28;107(7):905-16
references.pmid: 11779466
references.citation: Genetics. 2002 Oct;162(2):747-53
references.pmid: 12399385
references.citation: Nature. 2007 May 3;447(7140):102-5
references.pmid: 17410126
references.citation: Genetics. 1992 Sep;132(1):113-23
references.pmid: 1398048
references.citation: Science. 2012 Jun 22;336(6088):1588-90
references.pmid: 22723424
references.citation: Genetics. 1978 Jul;89(3):467-97
references.pmid: 17176535
references.citation: Genetics. 2004 Jul;167(3):1143-53
references.pmid: 15280230
references.citation: Genetics. 1967 Dec;57(4):767-82
references.pmid: 6082621
references.citation: Genetics. 1998 Jan;148(1):113-22
references.pmid: 9475725
references.citation: Genetics. 1996 May;143(1):129-36
references.pmid: 8722768
references.citation: PLoS Biol. 2007 Dec;5(12):e324
references.pmid: 18076285
references.citation: Fungal Genet Biol. 2012 Aug;49(8):619-25
references.pmid: 22691725
references.citation: Fungal Genet Biol. 2004 Oct;41(10):897-910
references.pmid: 15341912
references.citation: Genetics. 1994 May;137(1):19-39
references.pmid: 8056309
references.citation: Mol Genet Genomics. 2012 Feb;287(2):155-65
references.pmid: 22203161
references.citation: Nature. 1989 Mar 2;338(6210):35-9
references.pmid: 2537472
references.citation: Fungal Genet Biol. 2010 Oct;47(10):847-54
references.pmid: 20627132
references.citation: Genetics. 1992 Dec;132(4):975-85
references.pmid: 1334021
references.citation: Mol Cell Biol. 2000 Nov;20(21):7839-44
references.pmid: 11027255
references.citation: Curr Genet. 2004 May;45(5):289-301
references.pmid: 15007624
references.citation: Genetics. 1970 Nov;66(3):429-47
references.pmid: 5519650
references.citation: Aust J Biol Sci. 1974 Apr;27(2):219-29
references.pmid: 4277978
references.citation: BMC Genomics. 2009;10:475
references.pmid: 19832984
references.citation: Curr Genet. 1999 Jul;35(6):631-7
references.pmid: 10467008
references.citation: Curr Genet. 1995 Jul;28(2):155-63
references.pmid: 8590467
references.citation: Fungal Genet Biol. 2007 Jul;44(7):575-84
references.pmid: 17475521
references.citation: Plant Cell. 2012 Apr;24(4):1448-64
references.pmid: 22547783
references.citation: Cell. 1991 Mar 22;64(6):1155-61
references.pmid: 2004421
references.citation: Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1325-41
references.pmid: 290446
references.citation: Genetics. 1999 Nov;153(3):1271-83
references.pmid: 10545458
references.citation: Genes Dev. 2004 Jan 15;18(2):117-25
references.pmid: 14752007
references.citation: Cell. 1983 May;33(1):25-35
references.pmid: 6380756
references.citation: Genetics. 2000 Apr;154(4):1913-6
references.pmid: 10950640
references.citation: Nature. 2008 Jul 24;454(7203):479-85
references.pmid: 18615017
references.citation: PLoS Genet. 2006 Sep 22;2(9):e155
references.pmid: 17002499
references.citation: Mutat Res. 2000 Jun 30;451(1-2):129-50
references.pmid: 10915869
references.citation: Genetics. 2008 Mar;178(3):1251-69
references.pmid: 18385111
references.citation: Cell. 2007 Jul 27;130(2):259-72
references.pmid: 17662941
references.citation: Nucleic Acids Res. 2002 Jan 15;30(2):E2
references.pmid: 11788728
references.citation: Nature. 1989 Jul 27;340(6231):318-20
references.pmid: 2546083
references.citation: Genetics. 2003 May;164(1):81-94
references.pmid: 12750322
references.citation: Genetics. 1974 Mar;76(3):453-75
references.pmid: 4208856
references.citation: Science. 2012 Dec 7;338(6112):1363-5
references.pmid: 23224558
references.citation: PLoS Genet. 2011 Sep;7(9):e1002305
references.pmid: 21980306
references.citation: Genetics. 2004 Dec;168(4):1805-16
references.pmid: 15611158
references.citation: Genetics. 2004 Sep;168(1):35-48
references.pmid: 15454525
references.citation: Genetics. 2001 Dec;159(4):1511-25
references.pmid: 11779793
references.citation: Genetics. 2001 Oct;159(2):571-9
references.pmid: 11606534
references.citation: Gene. 1994 Dec 2;150(1):159-62
references.pmid: 7959044