title: Functional analysis of three BrMYB28 transcription factors controlling the biosynthesis of glucosinolates in Brassica rapa.
abstract: Glucosinolates (GSLs) are secondary metabolites that have anticarcinogenic activity and play defense roles in plants of the Brassicaceae family. MYB28 is known as a transcription factor that regulates aliphatic GSL biosynthesis in Arabidopsis thaliana. Brassicaceae plants have three orthologous copies of AtMYB28 derived from recent genome triplication. These BrMYB28 genes have a high level of sequence homology, with 81-87% similarities in the coding DNA sequence compared to Arabidopsis. Overexpression of three paralogous BrMYB28 genes in transgenic Chinese cabbage increased the total GSL content in all T1 generation plants and in two inbred lines of homozygous T2 plants. The highest total GSL contents were detected in homozygous T2 lines overexpressing BrMYB28.1, which showed an approximate fivefold increase compared to that of nontransgenic plants. The homozygous T2 lines with overexpressed BrMYB28.1 also showed an increased content of aliphatic, indolic, and aromatic GSLs compared to that of nontransgenic plants. Furthermore, all of the three BrMYB28 genes were identified as negative regulators of BrAOP2 and positive regulators of BrGSL-OH in the homozygous T2 lines. These data indicate the regulatory mechanism of GSL biosynthesis in B. rapa is unlike that in A. thaliana. Our results will provide useful information for elucidating the regulatory mechanism of GSL biosynthesis in polyploid plants.
authors.affiliation: Genomics Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration (RDA), Wansan-gu, Jeonju, Korea. sms1030@korea.kr.
authors.forename: Mi-Suk
authors.initials: MS
authors.lastname: Seo
authors.affiliation: Genomics Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration (RDA), Wansan-gu, Jeonju, Korea. genemina@korea.kr.
authors.forename: Mina
authors.initials: M
authors.lastname: Jin
authors.affiliation: Department of Biological Environment and Chemistry, College of Agriculture and Life Science, Chungnam National University, Yuseong-gu, Daejeon, Korea. slaldinv2@gmail.com.
authors.forename: Jin-Hyuk
authors.initials: JH
authors.lastname: Chun
authors.affiliation: Department of Biological Environment and Chemistry, College of Agriculture and Life Science, Chungnam National University, Yuseong-gu, Daejeon, Korea. kimsunju@cnu.ac.kr.
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Genomics Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration (RDA), Wansan-gu, Jeonju, Korea. pbeom@korea.kr.
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Park
authors.affiliation: Genomics Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration (RDA), Wansan-gu, Jeonju, Korea. sohnseonghan@korea.kr.
authors.forename: Seong-Han
authors.initials: SH
authors.lastname: Shon
authors.affiliation: Genomics Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration (RDA), Wansan-gu, Jeonju, Korea. jsnkim@korea.kr.
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D006720:Homozygote; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Brassica rapa; Chinese cabbage; Glucosinolates; Transcription factor BrMYB28s; Transgenic plats
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