title: Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.
abstract: BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. BacMams have become an integral part of the recombinant mammalian gene expression toolbox in research labs worldwide. Construction of transfer vectors is straightforward using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV.
authors.affiliation: Platform Technology and Science, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA, 19426, USA. Jim.A.Fornwald@gsk.com.
authors.forename: James A
authors.initials: JA
authors.lastname: Fornwald
authors.affiliation: Platform Technology and Science, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA, 19426, USA.
authors.forename: Quinn
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Boyce
authors.affiliation: Platform Technology and Science, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA, 19426, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Ames
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000302:isolation & purification; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D061987:Sf9 Cells; D018411:Spodoptera; D014170:Transformation, Genetic
chemical_list: D018076:DNA, Complementary; D004262:DNA Restriction Enzymes
keywords: BacMam; Co-expression; Mammalian expression; Modified baculovirus; Pseudotyping; Transient expression; Viral transduction; Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE)