title: E-cadherin maintains the activity of neural stem cells and inhibits the migration.
abstract: Cadherins are important adhesion molecules that mediate adhesions and communications between cells. These molecules participate in the formation and maintenance of multicellular organisms including the stem cells. E-cadherin is one of the classic cadherins which is reported to be essential for the survival and self-renewal of embryonic stem cells. Moreover, it could induce cell proliferation inhibitory signaling to regulate cell proliferation. In our study, we over-expressed and silenced E-cadherin in NSCs by lentiviral ways. Transgenic cells were confirmed by both quantitative RT-PCR and western blot. Results of MTT assay showed that over-expression of E-cadherin could enhance the cell activity. Furthermore, we performed Transwell chamber assay to analyze its role in regulation of cell migration. The results showed that the migration percent of over-expression cells was lower than control. Our results indicated that E-caherin would maintain the stemness of NSCs and reduce cells migration.
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Lin-Jie
authors.initials: LJ
authors.lastname: Luo
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Qian
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism*; D010641:Phenotype; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D015820:Cadherins; C000611838:Cdh1 protein, mouse; C489083:Cdh2 protein, mouse
keywords: Cadherins; E-cadherin; N-cadherin; cell activity; cell migration; neural stem cell
references.citation: Cell Adh Migr. 2012 Jan-Feb;6(1):59-70
references.pmid: 22647941
references.citation: Dev Cell. 2010 Mar 16;18(3):472-9
references.pmid: 20230753
references.citation: F1000Res. 2015 Aug 12;4(F1000 Faculty Rev):550
references.pmid: 26339481
references.citation: Mol Biol Cell. 2007 Jun;18(6):2013-25
references.pmid: 17392517
references.citation: Nat Biotechnol. 2007 Jun;25(6):681-6
references.pmid: 17529971
references.citation: Carcinogenesis. 2014 Apr;35(4):747-59
references.pmid: 24531939
references.citation: J Cell Biol. 2011 Jun 27;193(7):1137-46
references.pmid: 21708975
references.citation: EMBO J. 2007 Nov 14;26(22):4744-55
references.pmid: 17948051
references.citation: J Cell Biol. 2001 May 28;153(5):1049-60
references.pmid: 11381089
references.citation: J Clin Invest. 2002 Apr;109(8):987-91
references.pmid: 11956233
references.citation: Curr Top Dev Biol. 2015;112:129-96
references.pmid: 25733140
references.citation: Cancer Res. 2007 Dec 1;67(23):11254-62
references.pmid: 18056451
references.citation: J Cell Sci. 2014 Jun 15;127(Pt 12):2603-13
references.pmid: 24931943
references.citation: Cell Commun Adhes. 2013 Dec;20(6):189-99
references.pmid: 24205986
references.citation: J Cell Biol. 1994 Oct;127(1):247-56
references.pmid: 7929567