title: Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain.
abstract: Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.
authors.affiliation: Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.affiliation: Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Stephany Y
authors.initials: SY
authors.lastname: Tzeng
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Tammia
authors.affiliation: Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
authors.forename: Yu-Hao
authors.initials: YH
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Rolfe
authors.affiliation: Department of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Jordan J
authors.initials: JJ
authors.lastname: Green
authors.affiliation: Department of Chemical and Life Science Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA. Electronic address: xwen@vcu.edu.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wen
authors.affiliation: Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA. Electronic address: hmao@jhu.edu.
authors.forename: Hai-Quan
authors.initials: HQ
authors.lastname: Mao
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D002454:Cell Differentiation / Q000235:genetics; D002470:Cell Survival; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation*; D009474:Neurons / Q000166:cytology*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011923:Rats, Nude; D033581:Stem Cell Transplantation; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D011108:Polymers; C507253:poly(beta-amino ester)
keywords: Nanoparticles; Neurogenin-2; Neuronal differentiation; Transfection; Traumatic brain injury
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