title: Performance of rapid diagnostic tests for the detection of antibodies to hepatitis C virus in whole blood collected on dried blood spots.
abstract: Rapid diagnostic tests (RDTs) represent an attractive alternative to enzyme immunoassays. A total of 207 individuals, including 68 HCV-seronegative subjects, 10 patients with resolved infection and 129 patients with chronic HCV infection, were studied. The specificity of RDT detection of anti-HCV antibodies in whole blood was 100% with the four RDTs tested: OraQuick(®) HCV Rapid Antibody Test, First Response HCV Card Test, ASSURE HCV Rapid Test and MultiSure HCV Antibody Assay. Their diagnostic sensitivity varied between 98.6% and 100%. RDT detection of anti-HCV antibody in whole blood collected on dried blood spots appears to be a promising new tool for broadscale screening of HCV infection in high- to medium-risk populations.
authors.affiliation: National Reference Center for Viral Hepatitis B, C and delta, Department of Virology, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.|INSERM U955, Créteil, France.
authors.forename: L
authors.initials: L
authors.lastname: Poiteau
authors.affiliation: National Reference Center for Viral Hepatitis B, C and delta, Department of Virology, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.|INSERM U955, Créteil, France.
authors.forename: A
authors.initials: A
authors.lastname: Soulier
authors.affiliation: Department of Hepatology and Gastroenterology, Centre Intercommunal de Créteil, Créteil, France.
authors.forename: I
authors.initials: I
authors.lastname: Rosa
authors.affiliation: INSERM U955, Créteil, France.|Department of Public Health, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.
authors.forename: F
authors.initials: F
authors.lastname: Roudot-Thoraval
authors.affiliation: INSERM U955, Créteil, France.|Department of Hepatology and Gastroenterology, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.
authors.forename: C
authors.initials: C
authors.lastname: Hézode
authors.affiliation: National Reference Center for Viral Hepatitis B, C and delta, Department of Virology, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.|INSERM U955, Créteil, France.
authors.forename: J-M
authors.initials: JM
authors.lastname: Pawlotsky
authors.affiliation: National Reference Center for Viral Hepatitis B, C and delta, Department of Virology, Hôpital Henri Mondor, Université Paris-Est, Créteil, France.|INSERM U955, Créteil, France.
authors.forename: S
authors.initials: S
authors.lastname: Chevaliez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002846:Chromatography, Affinity / Q000379:methods*; D003890:Desiccation; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D018937:Hepatitis C Antibodies / Q000097:blood*; D019698:Hepatitis C, Chronic / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D055815:Young Adult
chemical_list: D018937:Hepatitis C Antibodies
keywords: anti-HCV antibodies; hepatitis C; rapid diagnostic test; screening