title: Differential Protein Expression Profiles in Glaucomatous Trabecular Meshwork: An Evaluation Study on a Small Primary Open Angle Glaucoma Population.
abstract: BACKGROUND
  Primary open angle glaucoma (POAG) is a progressive optic neuropathy characterized by impaired aqueous outflow and extensive remodeling in the trabecular meshwork (TM). The aim of this study was to characterize and compare the expression patterns of selected proteins belonging to the tissue remodeling, inflammation and growth factor pathways in ex vivo glaucomatous and post-mortem TMs using protein-array analysis.
  
  
  METHODS
  TM specimens were collected from 63 white subjects, including 40 patients with glaucoma and 23 controls. Forty POAG TMs were collected at the time of surgery and 23 post-mortem specimens were from non-glaucomatous donor sclerocorneal tissues. Protein profiles were evaluated using a chip-based array consisting of 60 literature-selected antibodies.
  
  
  RESULTS
  A different expression of some factors was observed in POAG TMs with respect to post-mortem specimens, either in abundance (interleukin [IL]10, IL6, IL5, IL7, IL12, IL3, macrophage inflammatory protein [MIP]1δ/α, vascular endothelial growth factor [VEGF], transforming growth factor beta 1 [TGFβ1], soluble tumor necrosis factor receptor I [sTNFRI]) or in scarcity (IL16, IL18, intercellular adhesion molecule 3 [ICAM3], matrix metalloproteinase-7 [MMP7], tissue inhibitor of metalloproteinase 1 [TIMP1]). MMP2, MMP7, TGFβ1, and VEGF expressions were confirmed by Western blot, zymography, and polymerase chain reaction. No difference in protein profile expression was detected between glaucomatous subtypes.
  
  
  CONCLUSIONS
  The analysis of this small TM population highlighted some proteins linked to POAG, some previously reported and others of new detection (IL7, MIPs, sTNFαRI). A larger POAG population is required to select promising disease-associated biomarker candidates.
  
  
  BACKGROUND
  This study was partially supported by the Fondazione Roma, the Italian Ministry of Health and the "National 5xMille 2010 tax donation to IRCCS-G.B. Bietti Foundation".
authors.affiliation: IRCCS-G.B. Bietti Foundation, Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Micera
authors.affiliation: Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, Section of Ophthalmology, University of Brescia, Brescia, Italy. luciano.quaranta@unibs.it.
authors.forename: Luciano
authors.initials: L
authors.lastname: Quaranta
authors.affiliation: IRCCS-G.B. Bietti Foundation, Rome, Italy.
authors.forename: Graziana
authors.initials: G
authors.lastname: Esposito
authors.affiliation: Laboratory of Clinical Research, IRCCS, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Floriani
authors.affiliation: San Giovanni Hospital Complex, Eye Bank, Rome, Italy.
authors.forename: Augusto
authors.initials: A
authors.lastname: Pocobelli
authors.affiliation: Ophthalmology Unit, Department of Head and Neck Pathologies, St. Martino Hospital, Genoa, Italy.
authors.forename: Sergio Claudio
authors.initials: SC
authors.lastname: Saccà
authors.affiliation: Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, Section of Ophthalmology, University of Brescia, Brescia, Italy.
authors.forename: Ivano
authors.initials: I
authors.lastname: Riva
authors.affiliation: Department of Clinical Science and Translational Medicine, University Tor Vergata, Rome, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Manni
authors.affiliation: IRCCS-G.B. Bietti Foundation, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Oddone
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D005260:Female; D005902:Glaucoma, Open-Angle / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D040081:Protein Array Analysis; D014129:Trabecular Meshwork / Q000378:metabolism*
chemical_list: D018836:Inflammation Mediators
keywords: Extracellular matrix; Intraocular pressure; Primary open angle glaucoma; Trabecular meshwork
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