title: Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats.
abstract: The noninvasive assessment of flap viability in autologous reconstruction surgery is still an unmet clinical need. To cope with this problem, we developed a proof-of-principle fully automatized setup for fast time-gated diffuse optical tomography exploiting Mellin-Laplace transform to obtain three-dimensional tomographic reconstructions of oxy- and deoxy-hemoglobin concentrations. We applied this method to perform preclinical tests on rats inducing total venous occlusion in the cutaneous abdominal flaps. Notwithstanding the use of just four source-detector couples, we could detect a spatially localized increase of deoxyhemoglobin following the occlusion (up to 550 μM in 54 min). Such capability to image spatio-temporal evolution of blood perfusion is a key issue for the noninvasive monitoring of flap viability.
authors.affiliation: Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Di Sieno
authors.affiliation: Hôpital d'Annecy, 1 avenue de l'hôpital, Metz Tessy, BP 90074-74374 Pringy cedex 9, FrancecUniversity Grenoble Alpes-site santé, Institut Albert Bonniot, INSERM U1209, Domaine de la merci, 38000, La Tronche, FrancedUniversity Grenoble Alpes, BP53, 38041 G.
authors.forename: Georges
authors.initials: G
authors.lastname: Bettega
authors.affiliation: CEA-LETI, Minatec Campus, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Berger
authors.affiliation: University Grenoble Alpes-site santé, Institut Albert Bonniot, INSERM U1209, Domaine de la merci, 38000, La Tronche, FrancedUniversity Grenoble Alpes, BP53, 38041 Grenoble, FrancefCentre Hospitalier Universitaire-Grenoble, Boulevard de la Chantourne, 3870.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Hamou
authors.affiliation: University Grenoble Alpes-site santé, Institut Albert Bonniot, INSERM U1209, Domaine de la merci, 38000, La Tronche, FrancedUniversity Grenoble Alpes, BP53, 38041 Grenoble, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Aribert
authors.affiliation: Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Alberto Dalla
authors.initials: AD
authors.lastname: Mora
authors.affiliation: University Grenoble Alpes-site santé, Institut Albert Bonniot, INSERM U1209, Domaine de la merci, 38000, La Tronche, FrancedUniversity Grenoble Alpes, BP53, 38041 Grenoble, FranceeCEA-LETI, Minatec Campus, 17 rue des Martyrs, 38054 Grenoble Cedex 9, Franc.
authors.forename: Agathe
authors.initials: A
authors.lastname: Puszka
authors.affiliation: CEA-LETI, Minatec Campus, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Grateau
authors.affiliation: Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Contini
authors.affiliation: CEA-LETI, Minatec Campus, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Hervé
authors.affiliation: University Grenoble Alpes-site santé, Institut Albert Bonniot, INSERM U1209, Domaine de la merci, 38000, La Tronche, FrancedUniversity Grenoble Alpes, BP53, 38041 Grenoble, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Coll
authors.affiliation: CEA-LETI, Minatec Campus, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Dinten
authors.affiliation: Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, 20133 Milano, ItalygIstituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pifferi
authors.affiliation: CEA-LETI, Minatec Campus, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Planat-Chrétien
mesh_terms: D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013524:Surgical Flaps / Q000502:physiology*; D041622:Tomography, Optical / Q000379:methods*