title: Distinctive effects of CD34- and CD133-specific antibody-coated stents on re-endothelialization and in-stent restenosis at the early phase of vascular injury.
abstract: It is not clear what effects of CD34- and CD133-specific antibody-coated stents have on re-endothelialization and in-stent restenosis (ISR) at the early phase of vascular injury. This study aims at determining the capabilities of different coatings on stents (e.g. gelatin, anti-CD133 and anti-CD34 antibodies) to promote adhesion and proliferation of endothelial progenitor cells (EPCs). The in vitro study revealed that the adhesion force enabled the EPCs coated on glass slides to withstand flow-induced shear stress, so that allowing for the growth of the cells on the slides for 48 h. The in vivo experiment using a rabbit model in which the coated stents with different substrates were implanted showed that anti-CD34 and anti-CD133 antibody-coated stents markedly reduced the intima area and restenosis than bare mental stents (BMS) and gelatin-coated stents. Compared with the anti-CD34 antibody-coated stents, the time of cells adhesion was longer and earlier present in the anti-CD133 antibody-coated stents and anti-CD133 antibody-coated stents have superiority in re-endothelialization and inhibition of ISR. In conclusion, this study demonstrated that anti-CD133 antibody as a stent coating for capturing EPCs is better than anti-CD34 antibody in promoting endothelialization and reducing ISR.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Xue
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Tieying
authors.initials: T
authors.lastname: Yin
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Tang
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Junli
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Deng
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Donghong
authors.initials: D
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400044, China, School of Biological Science and Medical Engineering, Beihang University, No. 37 XueYuan Road, HaiDian District, Beijing 100191, China and Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Fredrik Bajers Vej 7, Building H, 9220, Aalborg, Denmark.
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
keywords: CD133; CD34; endothelial progenitor cells; re-endothelialization; stent
references.citation: Microvasc Res. 2010 May;79(3):207-16
references.pmid: 20188747
references.citation: Circulation. 1997 Jan 21;95(2):438-48
references.pmid: 9008462
references.citation: Cardiovasc Res. 2010 May 1;86(2):302-10
references.pmid: 20032081
references.citation: Circulation. 2008 Nov 25;118(22):2277-85, 7p following 2285
references.pmid: 19001019
references.citation: J Vasc Surg. 2008 Mar;47(3):662-70
references.pmid: 17950562
references.citation: Biomaterials. 2012 Dec;33(35):8917-27
references.pmid: 22981075
references.citation: Clin Res Cardiol. 2013 Jun;102(6):413-25
references.pmid: 23397592
references.citation: N Engl J Med. 2004 Jan 15;350(3):221-31
references.pmid: 14724301
references.citation: Am Heart J. 2008 Jan;155(1):128-32
references.pmid: 18082503
references.citation: Science. 1997 Feb 14;275(5302):964-7
references.pmid: 9020076
references.citation: J Biomed Mater Res A. 2008 Dec 15;87(4):964-71
references.pmid: 18257077
references.citation: Circulation. 2003 Mar 4;107(8):1164-9
references.pmid: 12615796
references.citation: N Engl J Med. 2002 Jun 6;346(23 ):1773-80
references.pmid: 12050336
references.citation: Circulation. 2003 Jan 28;107(3):461-8
references.pmid: 12551872
references.citation: J Biomed Mater Res A. 2014 Feb;102(2):588-609
references.pmid: 23520056
references.citation: Basic Res Cardiol. 2010 Nov;105(6):713-24
references.pmid: 20830586
references.citation: Circulation. 2004 Apr 13;109 (14 ):1769-75
references.pmid: 15066951
references.citation: Catheter Cardiovasc Interv. 2008 Apr 1;71(5):600-4
references.pmid: 18360849
references.citation: J Appl Biomater Biomech. 2011 Sep-Dec;9(3):193-8
references.pmid: 21445830
references.citation: J Vasc Surg. 2011 Feb;53(2):461-71
references.pmid: 21129910
references.citation: J Biomed Mater Res A. 2014 Oct;102(10):3754-72
references.pmid: 24243819
references.citation: J Biomed Mater Res A. 2013 Apr;101(4):1144-57
references.pmid: 23045161
references.citation: Am J Cardiol. 1984 Jun 15;53(12 ):77C-81C
references.pmid: 6233894
references.citation: Biochem Biophys Res Commun. 1994 Jul 29;202(2):866-72
references.pmid: 7519425
references.citation: Circulation. 1998 Jul 21;98(3):224-33
references.pmid: 9697822
references.citation: Biochim Biophys Acta. 2012 Oct;1821(10 ):1361-9
references.pmid: 22841831
references.citation: Circ Res. 2003 Jul 25;93(2):e17-24
references.pmid: 12829619
references.citation: J Am Coll Cardiol. 2005 May 17;45(10):1574-9
references.pmid: 15893169