title: Bioadsorption of Rare Earth Elements through Cell Surface Display of Lanthanide Binding Tags.
abstract: With the increasing demand for rare earth elements (REEs) in many emerging clean energy technologies, there is an urgent need for the development of new approaches for efficient REE extraction and recovery. As a step toward this goal, we genetically engineered the aerobic bacterium Caulobacter crescentus for REE adsorption through high-density cell surface display of lanthanide binding tags (LBTs) on its S-layer. The LBT-displayed strains exhibited enhanced adsorption of REEs compared to cells lacking LBT, high specificity for REEs, and an adsorption preference for REEs with small atomic radii. Adsorbed Tb(3+) could be effectively recovered using citrate, consistent with thermodynamic speciation calculations that predicted strong complexation of Tb(3+) by citrate. No reduction in Tb(3+) adsorption capacity was observed following citrate elution, enabling consecutive adsorption/desorption cycles. The LBT-displayed strain was effective for extracting REEs from the acid leachate of core samples collected at a prospective rare earth mine. Our collective results demonstrate a rapid, efficient, and reversible process for REE adsorption with potential industrial application for REE enrichment and separation.
authors.affiliation: Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory , Livermore, California 92550, United States.
authors.forename: Dan M
authors.initials: DM
authors.lastname: Park
authors.affiliation: Idaho National Laboratory , Idaho Falls, Idaho 83415, United States.
authors.forename: David W
authors.initials: DW
authors.lastname: Reed
authors.affiliation: Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory , Livermore, California 92550, United States.
authors.forename: Mimi C
authors.initials: MC
authors.lastname: Yung
authors.affiliation: OLI Systems, Inc. , 240 Cedar Knolls Road, Suite 301, Cedar Knolls, New Jersey 07927, United States.
authors.forename: Ali
authors.initials: A
authors.lastname: Eslamimanesh
authors.affiliation: OLI Systems, Inc. , 240 Cedar Knolls Road, Suite 301, Cedar Knolls, New Jersey 07927, United States.
authors.forename: Malgorzata M
authors.initials: MM
authors.lastname: Lencka
authors.affiliation: OLI Systems, Inc. , 240 Cedar Knolls Road, Suite 301, Cedar Knolls, New Jersey 07927, United States.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Anderko
authors.affiliation: Idaho National Laboratory , Idaho Falls, Idaho 83415, United States.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fujita
authors.affiliation: Department of Materials Science and Engineering, Rutgers, The State University of New Jersey , 607 Taylor Road, Piscataway, New Jersey 08855, United States.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Riman
authors.affiliation: Peter A. Rock Thermochemistry Laboratory and NEAT ORU, University of California Davis , Davis, California 95616, United States.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Navrotsky
authors.affiliation: Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory , Livermore, California 92550, United States.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Jiao
mesh_terms: D000327:Adsorption; D016935:Caulobacter crescentus / Q000235:genetics / Q000378:metabolism*; D019343:Citric Acid / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods; D028581:Lanthanoid Series Elements / Q000378:metabolism*; D008674:Metals, Rare Earth / Q000302:isolation & purification / Q000378:metabolism*; D008906:Mining / Q000379:methods; D013725:Terbium / Q000378:metabolism
chemical_list: D028581:Lanthanoid Series Elements; D008674:Metals, Rare Earth; D013725:Terbium; D019343:Citric Acid
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