title: Thermal and chemical unfolding pathways of PaSdsA1 sulfatase, a homo-dimer with topologically interlinked chains.
abstract: Understanding the mechanisms as to how interlinked proteins entangle and fold is a challenge. PaSdsA1 sulfatase is a homo-dimer containing two zinc atoms per monomer. The monomer chains are interlinked in a dimerization domain. To study the unfolding pathways denaturation experiments were performed. In the native protein three forms coexist in chemical equilibrium, each with a different number of zinc atoms. In the chemical unfolding of the holo-dimers the entanglement of the chains is preserved and acts as a 'folding seed', allowing the unfolding process to be reversible. Thermal irreversible unfolding of the holo-dimers favours dissociation, producing monomers that are SDS-stabilized. The thermal unfolding of these monomers is reversible. However, it is not possible to form dimers from unfolded monomers.
authors.affiliation: Laboratorio de Biofisicoquímica, Departamento de Fisicoquímica, Facultad de Química, Universidad Nacional Autónoma de México, México D. F, México.
authors.forename: César
authors.initials: C
authors.lastname: Aguirre
authors.affiliation: Protein Folding Laboratory, Institute for Protein Research, Osaka University, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Laboratorio de Biofisicoquímica, Departamento de Fisicoquímica, Facultad de Química, Universidad Nacional Autónoma de México, México D. F, México.
authors.forename: Miguel
authors.initials: M
authors.lastname: Costas
mesh_terms: D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D019281:Dimerization; D008958:Models, Molecular; D011487:Protein Conformation; D058767:Protein Unfolding; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013429:Sulfatases / Q000737:chemistry*; D013696:Temperature
chemical_list: D012967:Sodium Dodecyl Sulfate; D013429:Sulfatases
keywords: PaSdsA1 sulfatase; entangled chains; interlinked chains; stabilization with SDS; unfolding pathways