title: Antimicrobial compounds from endophytic Streptomyces sp. BCC72023 isolated from rice (Oryza sativa L.).
abstract: An endophytic actinomycete strain BCC72023 was isolated from rice (Oryza sativa L.) and identified as the genus Streptomyces, based on phenotypic, chemotaxonomic and 16S rRNA gene sequence analyses. The strain showed 99.80% similarity compared with Streptomyces samsunensis M1463(T). Chemical investigation led to the isolation of three macrolides, efomycins M (1), G (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-O-methylabierixin (5). To our knowledge, this is the first report of efomycin M being isolated from a natural source. The compounds were identified using spectroscopic techniques and comparison with previously published data. All compounds exhibited antimalarial activity against the Plasmodium falciparum, K-1 strain, a multidrug-resistant strain, with IC50 values in a range of 1.40-5.23 μg/ml. In addition, these compounds were evaluated for biological activity against Mycobacterium tuberculosis, Bacillus cereus, Colletotrichum gloeosporioides and Colletotrichum capsici, as well as cytotoxicity against both cancerous (MCF-7, KB, NCI-H187) and non-cancerous (Vero) cells.
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand; Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Khomsan
authors.initials: K
authors.lastname: Supong
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand; Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Paholyothin Road, Klong Luang, Pathumthani 12120 Thailand.
authors.forename: Wilunda
authors.initials: W
authors.lastname: Choowong
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand; Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Chokchai
authors.initials: C
authors.lastname: Kittiwongwattana
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Dusanee
authors.initials: D
authors.lastname: Thanaboripat
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Chamroon
authors.initials: C
authors.lastname: Laosinwattana
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Prommart
authors.initials: P
authors.lastname: Koohakan
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Nonglak
authors.initials: N
authors.lastname: Parinthawong
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Paholyothin Road, Klong Luang, Pathumthani 12120 Thailand. Electronic address: pattamap@biotec.or.th.
authors.forename: Pattama
authors.initials: P
authors.lastname: Pittayakhajonwut
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D000970:Antineoplastic Agents / Q000032:analysis; D015373:Bacterial Typing Techniques; D001688:Biological Products / Q000032:analysis*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000737:chemistry*; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013057:Spectrum Analysis; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D001688:Biological Products; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Antimicrobial; Efomycins; Endophytic actinomycete; Macrolides; Secondary metabolites; Streptomyces