title: Bax inhibiting peptide reduces apoptosis in neonatal rat hypoxic-ischemic brain damage.
abstract: Neonatal hypoxic ischemic encephalopathy (HIE) has been reported to induce apoptosis in neonates. We, therefore, analyzed the ability of Bax-inhibiting peptide (BIP) to provide neuroprotective effects during hypoxic-ischemic brain damage (HIBD). Seven-day-old wistar rat pups (n = 198) were randomly divided into a sham-operated group (Group S, n = 18), saline group (Group C, n = 90) and BIP group (Group B, n = 90). Pathological changes in the cerebral tissues of rat pups were analyzed using hematoxylin and eosin stain, TUNEL and Western blot. The expression of cytochrome c and caspase-3 was determined using western blot technique. Rat pups demonstrated neurobehavioral alteration in Groups C and B. TUNEL-positive cells in the left hippocampus were significantly increased in Group C and Group B after HIBD (P < 0.01) when compared with Group S. There was a marked reduction in TUNEL positive cells in subgroups B1 through B4 when compared with the respective subgroups C1 through C5. Compared with Group S, the expression of caspase-3 and cytochrome c was significantly increased in Groups C and B (P < 0.01). The difference in expression of caspase-3 and cytochrome c between subgroups B1 through B4 and C1 through C4 was significant (P < 0.01). In conclusions, the neuro-protective effect of BIP was due to a reduction of nerve cell apoptosis in our neonatal HIE rat model. We propose that BIP has potential as a neuro-protective drug in neonatal HIE cases.
authors.affiliation: The Affiliated Hospital of Qingdao University 16th on Jiangsu Road, Shinan District, Qingdao 266003, Shandong Province, China.
authors.forename: Meng-Ya
authors.initials: MY
authors.lastname: Sun
authors.affiliation: Women and Children's Hospital of Qingdao 6th on Tongfu Road, Shibei District, Qingdao 266034, Shandong Province, China.
authors.forename: Kai-Jie
authors.initials: KJ
authors.lastname: Cui
authors.affiliation: Eighth People's Hospital of Qingdao No. 84 Fengshan Road, Licang District, Qingdao, Shandong Province, China.
authors.forename: Mao-Min
authors.initials: MM
authors.lastname: Yu
authors.affiliation: The Affiliated Hospital of Taian Medical College 706 on Mount Street, Tai'an 271000, Shandong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Affiliated Hospital of Qingdao University 16th on Jiangsu Road, Shinan District, Qingdao 266003, Shandong Province, China.
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Peng
authors.affiliation: The Affiliated Hospital of Qingdao University 16th on Jiangsu Road, Shinan District, Qingdao 266003, Shandong Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D007276:Injections, Intraventricular; D008297:Male; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C539791:Bax-inhibiting peptide, BIP; D018696:Neuroprotective Agents; D009842:Oligopeptides
keywords: Neonatal hypoxic ischemic encephalopathy; apoptosis; bax-inhibiting peptide; hypoxic-ischemic brain damage; necrosis
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