title: The effect of low-intensity pulsed ultrasound on bone-tendon junction healing: Initiating after inflammation stage.
abstract: The purpose of this study was to explore the effect of low-intensity pulsed ultrasound (LIPUS) treatment initiating after inflammation stage on the process of bone-tendon junction (BTJ) healing in a rabbit model. Thirty-six rabbits undergoing partial patellectomy were randomly divided into two groups: control and LIPUS. The period of initial inflammatory stage is 2 weeks. So LIPUS treatment was initiated at postoperative week 2 and continued until the patella-patellar tendon (PPT) complexes were harvested at postoperative weeks 4, 8, and 16. At each time point, the PPT complexes were harvested for qRT-PCR, histology, radiographs, synchroton radiation micro computed tomography (SR-µCT), and biomechanical testing. The qRT-PCR results showed that LIPUS treatment beginning at postoperative week 2 played an anti-inflammatory role in BTJ healing. Histologically, the LIPUS group showed more advanced remodeling of the lamellar bone and marrow cavity than the control group. The area and length of the new bone in the LIPUS group were significantly greater than the control group at postoperative weeks 8 and 16. SR-µCT demonstrated that new bone formation and remodeling in the LIPUS group were more advanced than the control group. Biomechanical test results demonstrated that the failure load, ultimate strength and energy at failure were significantly higher than those of the control group. In conclusion, LIPUS treatment beginning at postoperative week 2 was able to accelerate bone formation during the bone-tendon junction healing process and significantly improved the healing quality of BTJ injury. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1697-1706, 2016.
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Huabin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qu
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Daqi
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Jingyong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Spine Surgery, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. jianzhonghu@hotmail.com.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000528:radiation effects; D064594:Bone-Patellar Tendon-Bone Grafts / Q000528:radiation effects*; D005260:Female; D016133:Polymerase Chain Reaction; D011817:Rabbits; D011897:Random Allocation; D012038:Regeneration / Q000528:radiation effects*; D013710:Tendons / Q000528:radiation effects; D000069453:Ultrasonic Waves; D055114:X-Ray Microtomography
keywords: bone-tendon junction (BTJ); low-intensity pulsed ultrasound (LIPUS); synchroton radiation micro computed tomography (SR-µCT)
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