title: Incorporation of omics analyses into artificial gravity research for space exploration countermeasure development.
abstract: The next major steps in human spaceflight include flyby, orbital, and landing missions to the Moon, Mars, and near earth asteroids. The first crewed deep space mission is expected to launch in 2022, which affords less than 7 years to address the complex question of whether and how to apply artificial gravity to counter the effects of prolonged weightlessness. Various phenotypic changes are demonstrated during artificial gravity experiments. However, the molecular dynamics (genotype and molecular phenotypes) that underlie these morphological, physiological, and behavioral phenotypes are far more complex than previously understood. Thus, targeted molecular assessment of subjects under various G conditions can be expected to miss important patterns of molecular variance that inform the more general phenotypes typically being measured. Use of omics methods can help detect changes across broad molecular networks, as various G-loading paradigms are applied. This will be useful in detecting off-target, or unanticipated effects of the different gravity paradigms applied to humans or animals. Insights gained from these approaches may eventually be used to inform countermeasure development or refine the deployment of existing countermeasures. This convergence of the omics and artificial gravity research communities may be critical if we are to develop the proper artificial gravity solutions under the severely compressed timelines currently established. Thus, the omics community may offer a unique ability to accelerate discovery, provide new insights, and benefit deep space missions in ways that have not been previously considered.
authors.affiliation: Sovaris Aerospace, LLC, Advanced Pattern Analysis & Countermeasures Group, Research Innovation Center, Colorado State University, 3185 Rampart Road, Fort Collins, CO 80521 USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Schmidt
authors.affiliation: Disease Modeling and Tissue Analogues Laboratory, Biomedical Research and Environmental Sciences Division, NASA Lyndon B. Johnson Space Center, Houston, TX 77058 USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Goodwin
authors.affiliation: Chief Medical Officer, NASA Ames Research Center, Moffett Field, CA USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Pelligra
keywords: Artificial gravity; Astronaut; Countermeasures; Gravity; Omics; Space flight
references.citation: Neurosci Lett. 2003 Jan 23;336(3):147-50
references.pmid: 12505614
references.citation: Environ Health Perspect. 2012 Dec;120(12):1671-7
references.pmid: 23008272
references.citation: J Appl Physiol (1985). 2009 Jul;107(1):39-46
references.pmid: 19286573
references.citation: J Gravit Physiol. 1994 May;1(1):P100-1
references.pmid: 11538729
references.citation: J Leukoc Biol. 2012 Dec;92(6):1133-45
references.pmid: 22750545
references.citation: J Physiol. 2010 Sep 15;588(Pt 18):3567-92
references.pmid: 20660569
references.citation: Aerosp Med. 1965 Aug;36:733-54
references.pmid: 14334708
references.citation: Physiologist. 1992 Feb;35(1 Suppl):S49-50
references.pmid: 1589533
references.citation: J Mol Biol. 1999 Jul 9;290(2):447-57
references.pmid: 10390343
references.citation: J Endocrinol. 2000 Dec;167(3):417-28
references.pmid: 11115768
references.citation: Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2555-62
references.pmid: 24311596
references.citation: Int J Biochem Cell Biol. 2013 Oct;45(10):2163-72
references.pmid: 23806868
references.citation: J Biol Chem. 2011 Jul 1;286(26):23054-62
references.pmid: 21561855
references.citation: Eur J Appl Physiol. 2011 Jul;111(7):1235-60
references.pmid: 21161267
references.citation: Physiol Genomics. 2015 Apr;47(4):113-28
references.pmid: 25649141
references.citation: Annu Rev Biochem. 2001;70:369-413
references.pmid: 11395412
references.citation: Nucleic Acids Res. 2009 Feb;37(3):702-11
references.pmid: 19188255
references.citation: J Nutr. 2005 Mar;135(3):437-43
references.pmid: 15735075
references.citation: J Membr Biol. 1999 Mar 1;168(1):77-89
references.pmid: 10051691
references.citation: J Gravit Physiol. 1996 Sep;3(2):61-2
references.pmid: 11540284
references.citation: Nat Rev Cancer. 2013 May;13(5):315-27
references.pmid: 23584334
references.citation: J Biol Chem. 2007 Oct 19;282(42):30577-85
references.pmid: 17724035
references.citation: J Gravit Physiol. 2004 Mar;11(1):75-80
references.pmid: 16145813
references.citation: J Bone Miner Res. 2013 Apr;28(4):875-85
references.pmid: 23165526
references.citation: Nature. 2008 Feb 21;451(7181):949-52
references.pmid: 18288185
references.citation: Chem Soc Rev. 2009 Apr;38(4):1119-38
references.pmid: 19421584
references.citation: Aviat Space Environ Med. 1997 Sep;68(9):829-37
references.pmid: 9293353
references.citation: Acta Astronaut. 1980 Jan;7(1):113-21
references.pmid: 11542643
references.citation: Toxicol Sci. 2014 Jan;137(1):1-2
references.pmid: 24213143
references.citation: Exp Brain Res. 2001 Jun;138(4):410-8
references.pmid: 11465738
references.citation: Nat Protoc. 2011 Jun 30;6(7):1060-83
references.pmid: 21720319
references.citation: Oncogene. 2002 Dec 16;21(58):9022-32
references.pmid: 12483517
references.citation: Aviat Space Environ Med. 2014 Jul;85(7):694-9
references.pmid: 25022156
references.citation: Mutat Res. 2001 Apr 18;475(1-2):113-21
references.pmid: 11295157
references.citation: Semin Cancer Biol. 2005 Oct;15(5):353-64
references.pmid: 15982899
references.citation: Crit Rev Oncol Hematol. 2002 Apr;42(1):79-91
references.pmid: 11923070
references.citation: Cell Metab. 2009 Sep;10 (3):189-98
references.pmid: 19723495
references.citation: Biochim Biophys Acta. 2011 Jul;1813(7):1269-78
references.pmid: 20933024
references.citation: Biometals. 2002 Sep;15(3):225-35
references.pmid: 12206389
references.citation: Q Rev Biophys. 2008 Feb;41(1):41-101
references.pmid: 18755053
references.citation: Gravit Space Biol Bull. 1997 Jun;10(2):137-43
references.pmid: 11540114
references.citation: Am J Physiol. 1998 Sep;275(3 Pt 2):R879-88
references.pmid: 9728087
references.citation: Stress. 2008 Sep;11(5):339-47
references.pmid: 18800307
references.citation: Nutrients. 2015 Dec 08;7(12):10209-22
references.pmid: 26670248
references.citation: Arch Neurol. 1960 Jul;3:55-73
references.pmid: 13829134
references.citation: Aviat Space Environ Med. 2013 Nov;84(11):1191-5
references.pmid: 24279234
references.citation: Acta Astronaut. 2005 May-Jun;56(9-12):867-76
references.pmid: 15835033
references.citation: Trends Endocrinol Metab. 2012 Sep;23(9):459-66
references.pmid: 22817841
references.citation: Bull Exp Biol Med. 2013 Dec;156(2):201-4
references.pmid: 24319748
references.citation: Eur J Neurosci. 2004 Feb;19(3):667-77
references.pmid: 14984417
references.citation: J Gravit Physiol. 2007 Jul;14(1):P31-3
references.pmid: 18372689
references.citation: Am J Kidney Dis. 2001 Sep;38(3):668-74
references.pmid: 11532704
references.citation: Aging (Albany NY). 2014 Jan;6(1):9-25
references.pmid: 24457528
references.citation: PLoS One. 2014 Mar 24;9(3):e92664
references.pmid: 24664102
references.citation: J Affect Disord. 2013 Nov;151(2):679-85
references.pmid: 23992681
references.citation: J Strength Cond Res. 2015 Mar;29(3):863-8
references.pmid: 25226319
references.citation: J Bone Miner Res. 2005 Feb;20(2):208-18
references.pmid: 15647814
references.citation: Metabolomics. 2013;9(6):1134-1156
references.pmid: 24273472
references.citation: Nat Rev Neurol. 2009 Jun;5(6):311-22
references.pmid: 19498435
references.citation: Analyst. 2005 Apr;130(4):442-65
references.pmid: 15776152
references.citation: Physiol Genomics. 2000 Sep 08;3(3):163-73
references.pmid: 11015612
references.citation: Front Syst Neurosci. 2015 Jun 17;9:92
references.pmid: 26136665
references.citation: Int J Epidemiol. 2012 Feb;41(1):24-32
references.pmid: 22296988
references.citation: Science. 2010 Feb 12;327(5967):863-6
references.pmid: 20075215
references.citation: J Interferon Cytokine Res. 2014 Oct;34(10):778-86
references.pmid: 24702175
references.citation: Nat Med. 1999 Apr;5(4):359
references.pmid: 10202906