title: Isolation of Human Basophils.
abstract: Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population. We describe herein laboratory protocols that have been refined over the past ∼25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: John T
authors.initials: JT
authors.lastname: Schroeder
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: Anja P
authors.initials: AP
authors.lastname: Bieneman
mesh_terms: D000423:Alcian Blue; D001491:Basophils / Q000166:cytology*; D002469:Cell Separation; D002498:Centrifugation; D004396:Coloring Agents; D006801:Humans; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: D004396:Coloring Agents; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D000423:Alcian Blue
keywords: immune disease; immunologic studies in humans; in vitro assays for immune cell function; innate immunity
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