title: The Physiologic Effects of Isoflurane, Sevoflurane, and Hypothermia Used for Anesthesia in Neonatal Rats (Rattus norvegicus).
abstract: Information regarding effective anesthetic regimens for neonatal rat pups is limited. Here we investigated whether isoflurane or sevoflurane anesthesia maintains physiologic parameters more consistently than does hypothermia anesthesia in neonatal rat pups. Rat pups (age, 4 d) were randomly assigned to receive isoflurane, sevoflurane, or hypothermia. Physiologic parameters monitored at 1, 5, 10, and 15 min included heart rate (HR), respiratory rate (RR), and oxygen saturation (%SpO2). Other parameters evaluated were loss and return of righting reflex, paw withdrawal reflex, and maternal acceptance. Corticosterone and glucose were sampled at 20 min and 24 h after anesthesia induction. Once a surgical plane of anesthesia was achieved, a skin incision was made on the right lateral thigh. After the procedure, all pups were accepted and cared for by their dam. Isoflurane- and sevoflurane-treated pups maintained higher HR, RR, %SpO2, and glucose levels than did hypothermia-treated pups. For both the isoflurane and sevoflurane groups, HR and RR were significantly lower at 10 and 15 min after anesthesia than at 1 min. Compared with hypothermia, isoflurane and sevoflurane anesthesia provided shorter times to loss of and return of the righting reflex. Although corticosterone did not differ among the groups, glucose levels were higher at 20 min after anesthesia induction than at 24 h in all anesthetic groups. We conclude that both isoflurane and sevoflurane anesthesia maintain physiologic parameters (HR, RR, %SpO2) more consistently than does hypothermia anesthesia in 4-d-old rat pups.
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, California;, Email: monikag@stanford.edu.
authors.forename: Monika K
authors.initials: MK
authors.lastname: Huss
authors.affiliation: Department of Laboratory Animal Resources, University of California Berkeley, Berkeley, California.
authors.forename: Helen H
authors.initials: HH
authors.lastname: Chum
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, California.
authors.forename: Angela G
authors.initials: AG
authors.lastname: Chang
authors.affiliation: Department of Mathematics, Naresuan University, Phitsanulok, Thailand.
authors.forename: Katechan
authors.initials: K
authors.lastname: Jampachairsi
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, California.
authors.forename: Cholawat
authors.initials: C
authors.lastname: Pacharinsak
mesh_terms: D000769:Anesthesia, Inhalation; D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D007035:Hypothermia; D007530:Isoflurane / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D012018:Reflex / Q000187:drug effects; D056152:Respiratory Rate / Q000187:drug effects; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D007530:Isoflurane
references.citation: Crit Care Med. 2009 Mar;37(3):1101-20
references.pmid: 19237924
references.citation: Brain Res. 1986 Mar;396(1):64-76
references.pmid: 3011218
references.citation: J Comp Psychol. 1988 Mar;102(1):44-8
references.pmid: 3365944
references.citation: Anesthesiology. 2001 Sep;95(3):734-9
references.pmid: 11575548
references.citation: Lab Anim. 2000 Jul;34(3):260-4
references.pmid: 11037119
references.citation: Am J Physiol. 1984 Jul;247(1 Pt 1):E47-52
references.pmid: 6377920
references.citation: Br J Anaesth. 2000 Aug;85(2):305-7
references.pmid: 10992843
references.citation: Minerva Anestesiol. 2000 May;66(5):264-7
references.pmid: 10965699
references.citation: Exp Anim. 2004 Jan;53(1):63-5
references.pmid: 14993745
references.citation: J Clin Invest. 1941 Sep;20(5):521-33
references.pmid: 16694857
references.citation: Physiol Behav. 1990 Jun;47(6):1117-24
references.pmid: 2395915
references.citation: Br J Anaesth. 2005 Sep;95(3):393-9
references.pmid: 15994850
references.citation: Endocrinology. 1991 Mar;128(3):1385-95
references.pmid: 1847856
references.citation: Lab Anim Sci. 1997 Aug;47(4):386-95
references.pmid: 9306312
references.citation: Anesthesiology. 2009 Nov;111(5):1044-51
references.pmid: 19809283
references.citation: Respir Physiol Neurobiol. 2003 Aug 14;137(1):29-40
references.pmid: 12871675
references.citation: Dev Psychobiol. 1992 Nov;25(7):511-28
references.pmid: 1459345
references.citation: Respir Physiol. 1996 Feb;103(2):105-12
references.pmid: 8833542
references.citation: Behav Brain Res. 2005 Feb 10;157(1):45-53
references.pmid: 15617770
references.citation: J Neurosci Methods. 1993 Apr;47(1-2):105-14
references.pmid: 8321010
references.citation: Can J Anaesth. 1996 Nov;43(11):1090-4
references.pmid: 8922762
references.citation: Anesth Analg. 2006 Jan;102(1):75-80
references.pmid: 16368807
references.citation: Dev Psychobiol. 1984 May;17(3):255-62
references.pmid: 6539261
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 1999 Jul;123(3):221-34
references.pmid: 10501017
references.citation: Neuropsychopharmacology. 1996 Dec;15(6):587-94
references.pmid: 8946433
references.citation: Nat Protoc. 2006;1(6):3166-73
references.pmid: 17406580
references.citation: Cell Signal. 2014 May;26(5):1001-10
references.pmid: 24463006
references.citation: Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):187-92
references.pmid: 23248315
references.citation: Brain Res. 2010 Jan 11;1307:43-52
references.pmid: 19840780
references.citation: Neuroendocrinology. 1986;43(3):383-90
references.pmid: 3016585
references.citation: Paediatr Anaesth. 2005 Jul;15(7):574-9
references.pmid: 15960641
references.citation: Science. 2011 Feb 25;331(6020):1078-80
references.pmid: 21350179
references.citation: Plast Reconstr Surg. 1992 Jan;89(1):11-7; discussion 18-20
references.pmid: 1727240
references.citation: Neuroendocrinology. 1986;42(2):137-42
references.pmid: 3005896
references.citation: Anesthesiology. 1997 Apr;86(4):957-65
references.pmid: 9105240