title: Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis.
abstract: Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-γ and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.
authors.affiliation: 1 Department of Immunology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Stanisavljević
authors.affiliation: 2 Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia.
authors.forename: J
authors.initials: J
authors.lastname: Lukić
authors.affiliation: 1 Department of Immunology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Momčilović
authors.affiliation: 2 Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Miljković
authors.affiliation: 1 Department of Immunology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: B
authors.initials: B
authors.lastname: Jevtić
authors.affiliation: 2 Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Kojić
authors.affiliation: 2 Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia.
authors.forename: N
authors.initials: N
authors.lastname: Golić
authors.affiliation: 3 Institute for Microbiology and Immunology, School of Medicine, University of Belgrade, dr. Subotica 1, 11000 Belgrade, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Mostarica Stojković
authors.affiliation: 1 Department of Immunology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Miljković
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D000068536:Bacillota / Q000145:classification / Q000302:isolation & purification; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000276:immunology* / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000382:microbiology; D008198:Lymph Nodes / Q000276:immunology; D010581:Peyer's Patches / Q000276:immunology*; D051381:Rats; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D016207:Cytokines
keywords: DGGE; animal models; cytokine; faeces; gut-associated lymphoid tissues; immune system