title: Aberrant expression of angiopoietin-like proteins 1 and 2 in cumulus cells is potentially associated with impaired oocyte developmental competence in polycystic ovary syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine disorder associated with obesity, insulin resistance, hyperandrogenism, alterations in ovarian angiogenesis and impaired oocyte competence. Emerging evidence demonstrates that angiopoietin-like protein 1 (ANGPTL1) and angiopoietin-like protein 2 (ANGPTL2) have an important influence on angiogenesis, androgen biosynthesis, insulin resistance and adipocytes function. In this study, we set out to determine the potential relationship between ANGPTL1, ANGPTL2 and oocyte competence in PCOS through analyzing the expression levels and dynamic pattern of the two genes in cumulus cells (CCs) during different phases of nuclear maturation of PCOS patients and control groups undergoing controlled ovarian hyperstimulation (COH) for in vitro fertilization and embryo transfer. We found that the relative abundance of ANGPTL1 and ANGPTL2 transcripts in CCs from patients with PCOS showed dynamic changes during oocyte maturation. Specifically, their expressions were increased significantly at the Metaphase II stage. In summary, the present novel evidence indicates that the expression patterns of ANGPTL1 and ANGPTL2 mRNAs are disordered during oocyte maturation in PCOS, which were potentially related to aberrant oocyte quality and developmental potency, at least in part, via pathological angiogenesis and metabolism.
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China .
authors.forename: Zhenteng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: b Medical College of Shandong University , Jinan , Shandong , China , and.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China .
authors.forename: Cuifang
authors.initials: C
authors.lastname: Hao
authors.affiliation: c Department of Gynecology , Qingdao Women and Children Health Care Center , Qingdao , Shandong , China.
authors.forename: Qianwen
authors.initials: Q
authors.lastname: Xue
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China .
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China .
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China .
authors.forename: Hongchu
authors.initials: H
authors.lastname: Bao
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China .
authors.forename: Qinglan
authors.initials: Q
authors.lastname: Qu
mesh_terms: D000328:Adult; D000091304:Angiopoietin-Like Protein 1; D000091302:Angiopoietin-Like Protein 2; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000378:metabolism*; D054885:Cumulus Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D009866:Oogenesis / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*
chemical_list: C117578:ANGPTL1 protein, human; C485792:ANGPTL2 protein, human; D000091304:Angiopoietin-Like Protein 1; D000091302:Angiopoietin-Like Protein 2; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins
keywords: ANGPTL1; ANGPTL2; oocyte competence; polycystic ovary syndrome