title: Fish gelatin combined with chitosan coating inhibits myofibril degradation of golden pomfret (Trachinotus blochii) fillet during cold storage.
abstract: Coating of gelatin and chitosan can improve fish fillet's quality, but the mechanism is not clear. Chitosan/gelatin coatings significantly prevented deterioration of golden pomfret fillet at 4 °C. Chitosan with 7.2% gelatin group showed the best effect on preserving the length of myofibril, which remained greater than 15 μm at day 17 of storage, while for control, chitosan and chitosan combined with 3.6% gelatin group, it was 5.03, 10.04 and 9.02 μm, respectively. The MALDI-TOF MS result revealed that the coatings slowed down the protein deterioration of fillet. On days 13 and 17, the myosin light chain and myoglobin in control group degraded, while the two proteins still existed in chitosan/gelatin coated groups. Overall, the chitosan with 7.2% gelatin coating had the best effect on preserving fillet's quality during storage. The coating may exert its protective effect via inhibiting myofibril degradation within fillet.
authors.affiliation: Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, 117543 Singapore, Singapore; National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Brisbane 4072, Australia.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Bansal
authors.affiliation: Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, 117543 Singapore, Singapore; National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China. Electronic address: chmynghs@nus.edu.sg.
authors.forename: Hongshun
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D048271:Chitosan / Q000008:administration & dosage*; D005519:Food Preservation; D005780:Gelatin / Q000008:administration & dosage*; D009210:Myofibrils / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D017747:Seafood / Q000032:analysis*
chemical_list: D005780:Gelatin; D048271:Chitosan
keywords: Atomic force microscopy (AFM); Chitosan; Edible coating; Gelatin; Matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF-MS); Myofibril; Myoglobin; Myosin light chain