title: Association Between Serum Levels of Testosterone and Estradiol With Meibomian Gland Assessments in Postmenopausal Women.
abstract: OBJECTIVE
  The aims of this analysis were to determine if there is an association between serum levels of testosterone and estradiol with meibomian gland (MG) morphology and lipid layer thickness.
  
  
  METHODS
  The data used for this analysis were collected from postmenopausal women with and without dry eye disease. Meibography was used to assess MG dropout on the central two-thirds of the eyelid and biomicroscopy was used for assessing MG expressibility and meibum quality. Venous blood samples were drawn for serum hormone level analysis. The Kruskal-Wallis test and Spearman correlations were used for statistical analysis.
  
  
  RESULTS
  One hundred ninety-eight postmenopausal women with an average age of 61.2 (± 9.1) years were included in this analysis. Testosterone levels showed significant differences between MG dropout grades 1 and 4 (P = 0.002) and grades 2 and 4 (P = 0.01), whereas estradiol levels were different based on MG dropout (P = 0.53). No significant correlations were found between testosterone (r = 0.10, P = 0.17) or estradiol (r = 0.05, P = 0.45) and lipid layer thickness.
  
  
  CONCLUSIONS
  Testosterone levels were increased with MG dropout, which was significant between the mild and severe dropout groups, whereas no significant differences were found with estradiol and any MG assessment. Although the literature suggests an association of serum hormone levels and pathogenesis of dry eye disease in postmenopausal women, analysis of active sex steroid precursors and local tissue hormone levels may prove more useful.
authors.forename: Anna F
authors.initials: AF
authors.lastname: Ablamowicz
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Nichols
authors.forename: Kelly K
authors.initials: KK
authors.lastname: Nichols
mesh_terms: D000368:Aged; D015352:Dry Eye Syndromes / Q000097:blood*; D004958:Estradiol / Q000097:blood*; D005141:Eyelid Diseases / Q000097:blood / Q000000981:diagnostic imaging / Q000473:pathology*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008537:Meibomian Glands / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D017698:Postmenopause / Q000097:blood*; D011859:Radiography; D011795:Surveys and Questionnaires; D013666:Tears / Q000378:metabolism; D013739:Testosterone / Q000097:blood*
chemical_list: D013739:Testosterone; D004958:Estradiol
references.citation: Invest Ophthalmol Vis Sci. 2014 Apr;55(4):2614-8
references.pmid: 24692127
references.citation: Ophthalmologe. 2013 Mar;110(3):230-8
references.pmid: 23224122
references.citation: Br J Ophthalmol. 2000 Jan;84(1):76-84
references.pmid: 10611104
references.citation: Arch Ophthalmol. 1995 Oct;113(10):1266-70
references.pmid: 7575257
references.citation: J Clin Diagn Res. 2013 Oct;7(10):2207-10
references.pmid: 24298477
references.citation: Cornea. 1991 Jul;10(4):277-85
references.pmid: 1889213
references.citation: Eye (Lond). 1991;5 ( Pt 4):395-411
references.pmid: 1743355
references.citation: Optom Vis Sci. 2012 Mar;89(3):E310-5
references.pmid: 22246333
references.citation: Optom Vis Sci. 2008 Aug;85(8):675-83
references.pmid: 18677234
references.citation: Br J Ophthalmol. 2014 Mar;98(3):371-6
references.pmid: 24390165
references.citation: ISRN Ophthalmol. 2011 Oct 20;2011:465198
references.pmid: 24533183
references.citation: Ocul Surf. 2003 Jul;1(3):107-26
references.pmid: 17075643
references.citation: Invest Ophthalmol Vis Sci. 2011 Mar;52(4):1994-2005
references.pmid: 21450917
references.citation: Cornea. 1991 Jul;10(4):286-90
references.pmid: 1832371
references.citation: Invest Ophthalmol Vis Sci. 2010 May;51(5):2418-23
references.pmid: 20019370
references.citation: Ophthalmology. 2000 Jan;107(1):180-4
references.pmid: 10647739
references.citation: J Clin Endocrinol Metab. 2000 Dec;85(12):4874-82
references.pmid: 11134156
references.citation: Invest Ophthalmol Vis Sci. 2011 Mar;52(4):1930-7
references.pmid: 21450914
references.citation: Ophthalmology. 1985 Oct;92(10):1423-6
references.pmid: 4069605
references.citation: Invest Ophthalmol Vis Sci. 2011 Mar;52(4):1938-78
references.pmid: 21450915
references.citation: Curr Eye Res. 2008 May;33(5):405-20
references.pmid: 18568877
references.citation: Eye Contact Lens. 2009 Jul;35(4):203-8
references.pmid: 19516145
references.citation: J Clin Endocrinol Metab. 1976 Feb;42(2):247-53
references.pmid: 177438
references.citation: Cornea. 2010 Dec;29(12):1333-45
references.pmid: 20847669
references.citation: Invest Ophthalmol Vis Sci. 2013 Oct;54(11):TFOS37-70
references.pmid: 24058138
references.citation: Curr Eye Res. 2015 Feb;40(2):162-75
references.pmid: 25290221
references.citation: Optom Vis Sci. 2012 May;89(5):788-94
references.pmid: 22525129
references.citation: Am J Med. 2005 Dec 19;118 Suppl 12B:3-7
references.pmid: 16414321
references.citation: Cornea. 2005 May;24(4):382-8
references.pmid: 15829792
references.citation: Adv Exp Med Biol. 2002;506(Pt B):989-98
references.pmid: 12614022
references.citation: Am J Ophthalmol. 2003 Aug;136(2):318-26
references.pmid: 12888056
references.citation: Graefes Arch Clin Exp Ophthalmol. 2003 Sep;241(9):705-9
references.pmid: 12904971
references.citation: Eye Contact Lens. 2011 Sep;37(5):298-301
references.pmid: 21617536
references.citation: Ocul Surf. 2004 Apr;2(2):92-123
references.pmid: 17216082
references.citation: Br J Ophthalmol. 1984 Aug;68(8):524-8
references.pmid: 6743618
references.citation: Invest Ophthalmol Vis Sci. 2011 Mar;52(4):2006-49
references.pmid: 21450918
references.citation: Clin Ophthalmol. 2009;3:405-12
references.pmid: 19688028
references.citation: Invest Ophthalmol Vis Sci. 2008 May;49(5):1797-808
references.pmid: 18436814
references.citation: Invest Ophthalmol Vis Sci. 2003 Feb;44(2):533-9
references.pmid: 12556380
references.citation: Invest Ophthalmol Vis Sci. 2000 Nov;41(12):3732-42
references.pmid: 11053270
references.citation: Gynecol Endocrinol. 2005 May;20(5):289-98
references.pmid: 16019376
references.citation: Endocr Rev. 2003 Apr;24(2):152-82
references.pmid: 12700178
references.citation: Surv Ophthalmol. 2007 Jul-Aug;52(4):369-74
references.pmid: 17574063
references.citation: Exp Eye Res. 2006 Aug;83(2):291-6
references.pmid: 16579987
references.citation: J Clin Endocrinol Metab. 1974 Dec;39(6):1020-4
references.pmid: 4430702
references.citation: Ophthalmology. 1998 Aug;105(8):1485-8
references.pmid: 9709762