title: Characterization of Mycolic Acids in Total Fatty Acid Methyl Ester Fractions from Mycobacterium Species by High Resolution MALDI-TOFMS.
abstract: Mycolic acids (MAs) are characteristic components of bacteria in the suborder Corynebacterineae, such as Mycobacterium. MAs are categorized into subclasses based on their functional bases (cyclopropane ring, methoxy, keto, and epoxy group). Since MAs have heterogeneity among bacterial species, analyzing of MAs are required in the chemotaxonomic field. However, their structural analysis is not easy because of their long carbon-chain lengths and several functional groups. In this study, total fatty acid (FA) methyl ester (ME) fraction of M. tuberculosis H37Rv was analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOFMS) with a spiral ion trajectory (MALDI spiral-TOFMS). The distributions of carbon-chain length and their relative peak intensities were confirmed with those obtained by analysis of each subclass fraction which was separated from total FA ME fraction using thin-layer chromatography (TLC). The observed major peaks were reliably assigned as MAs owing to the high mass accuracy (error<3 ppm). The types of MA subclasses, their distributions of carbon-chain lengths, their relative peak intensities, and the ratio of even- and odd-numbered carbon-chain MAs for the total FA ME fraction were consistent with those of MA subclass fractions. To visualize whole MAs, contour maps of relative peak intensities for whole MAs were created. The contour maps indicated the MA subclasses and their distributions of carbon-chains with relative peak intensities at a glance. Our proposed method allows simple characterization in a short time and thus enables the analysis of large numbers of samples, and it would contribute to the chemotaxonomy.
authors.affiliation: Advanced and Fundamental Technology Center, JEOL Ltd.
authors.forename: Kanae
authors.initials: K
authors.lastname: Teramoto
authors.affiliation: Advanced and Fundamental Technology Center, JEOL Ltd.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Suga
authors.affiliation: MS Business Unit, JEOL Ltd.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of International Health, Institute of Tropical Medicine, Nagasaki University.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Wada
authors.affiliation: Osaka City Institute of Public Health and Environmental Sciences.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Department of Food and Nutrition, Faculty of Contemporary Human Life Science, Tezukayama University.
authors.forename: Nagatoshi
authors.initials: N
authors.lastname: Fujiwara
keywords: MALDI spiral-TOFMS; Mycobacterium; chemotaxonomy; contour map; mycolic acid
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