title: Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells.
abstract: Expression of recombinant proteins in insect cells infected with baculoviruses is commonplace. This system provides an easy way to generate a significant amount of properly folded, functional protein with proper posttranslational modifications and can be used to effectively produce multi-protein complexes. This chapter describes an alternative method of expressing high order protein complexes in insect cell culture. Specific examples involving the expression of 5- and 8-protein complexes are discussed.
authors.affiliation: Biological Sciences, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA, 19426, USA. Stephanie.B.Chen@gsk.com.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology* / Q000821:virology
chemical_list: D011994:Recombinant Proteins
keywords: BIIC; Baculovirus; Baculovirus infected insect cells; Co-expression; Multicomponent protein; Recombinant co-expression; Sf-9; Spodoptera frugiperda