title: Effect of tumor-associated macrophages on gastric cancer stem cell in omental milky spots and lymph node micrometastasis.
abstract: We observed whether the effect of tumor-associated macrophages on gastric cancer stem cell in omental milky spots and lymph nodes micrometastasis and research its possible mechanism. Macrophage THP-1 cells and Human gastric adenocarcinoma SGC-7901 cells were collectively cultivated in vivo. We found macrophage could suppress the proliferation and accelerated cell death of MFC cell. Meanwhile, these effects may be concerned with many signaling pathways, and we detected MCP-1 and COX-2 miRNA expressions, PGE-2 release levels, IL-4 and IL-10 activities, and TGF-β, IFN-γ, VEGF, MMP-2 and MMP-9 protein expressions in collectively cultivated cell. We found that MCP-1 and COX-2 miRNA expressions, and PGE-2 release levels were suppressed, IL-4 activity was inhibited and IL-10 activity was activated in collectively cultivated cell. Meanwhile, TGF-β, MMP-2 and MMP-9 protein expressions were inhibited and IFN-γ and VEGF protein expressions were activated in collectively cultivated cell. Taken together, these results suggest that the effect of tumor-associated macrophages on gastric cancer stem cell in omental milky spots and lymph nodes micrometastasis via COX-2/PGE-2/TGF-β/VEGF signal pathways.
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.affiliation: Liaoning Laboratory of Cancer Genetics and Epigenetics, Department of Cell Biology, Dalian Medical University Dalian, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Zheng-Lin
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Huan-Ran
authors.initials: HR
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey Dalian, China.
authors.forename: Xun-Guo
authors.initials: XG
authors.lastname: Yin
authors.affiliation: Department of Hepatobiliary Pancreas Surgery, The Second Affiliated Hospital of Dalian Medical University Dalian, China.
authors.forename: Cheng-Yong
authors.initials: CY
authors.lastname: Dong
authors.affiliation: Department of Hepatobiliary Pancreas Surgery, The Second Affiliated Hospital of Dalian Medical University Dalian, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D016923:Cell Death; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D061206:Neoplasm Micrometastasis; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D009852:Omentum / Q000378:metabolism* / Q000473:pathology; D019899:Paracrine Communication; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016212:Transforming Growth Factor beta; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone
keywords: COX-2; Tumor-associated macrophages; VEGF; gastric cancer stem cell
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