title: Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes.
abstract: Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from Enterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anti correlated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of E. faeciumon the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action of E. faeciumon immune cell regulation in the small intestine.
authors.affiliation: Faculty of Life Sciences, Thaer Institute, Breeding Biology and Molecular Genetics, Humboldt Universität zu Berlin, Berlin, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Kreuzer-Redmer
authors.affiliation: Faculty of Life Sciences, Thaer Institute, Breeding Biology and Molecular Genetics, Humboldt Universität zu Berlin, Berlin, Germany.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Bekurtz
authors.affiliation: Faculty of Life Sciences, Thaer Institute, Breeding Biology and Molecular Genetics, Humboldt Universität zu Berlin, Berlin, Germany.
authors.forename: Danny
authors.initials: D
authors.lastname: Arends
authors.affiliation: Faculty of Life Sciences, Thaer Institute, Breeding Biology and Molecular Genetics, Humboldt Universität zu Berlin, Berlin, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Bortfeldt
authors.affiliation: Department of Veterinary Medicine, Institute of Veterinary Biochemistry, Freie Universität Berlin, Berlin, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kutz-Lohroff
authors.affiliation: Department of Veterinary Medicine, Institute of Veterinary Biochemistry, Freie Universität Berlin, Berlin, Germany.
authors.forename: Soroush
authors.initials: S
authors.lastname: Sharbati
authors.affiliation: Department of Veterinary Medicine, Institute of Veterinary Biochemistry, Freie Universität Berlin, Berlin, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Einspanier
authors.affiliation: Faculty of Life Sciences, Thaer Institute, Breeding Biology and Molecular Genetics, Humboldt Universität zu Berlin, Berlin, Germany gudrun.brockmann@agrar.hu-berlin.de.
authors.forename: Gudrun A
authors.initials: GA
authors.lastname: Brockmann
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015536:Down-Regulation; D016984:Enterococcus faecium / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007136:Immunoglobulins / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine
chemical_list: D007136:Immunoglobulins; D035683:MicroRNAs
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