title: Cryptosporidium infections of ring-necked pheasants (Phasianus colchicus) from an intensive artificial breeding programme in the Czech Republic.
abstract: From July to November 2012, preliminary coprological examinations were carried out on 85 pooled faecal samples of different aged ring-necked pheasants (Phasianus colchicus) (hatches from May until July) from an intensive artificial breeding programme in the Czech Republic. Cryptosporidium oocysts were detected in 12 samples (14.1 %) of ages >12 weeks (August-September). These results were supported by findings of Cryptosporidium baileyi and Cryptosporidium meleagridis oocysts in intestinal, or cloacal contents, and/or the bursa of Fabricius in 9 from 36 examined dead pheasants (prevalence 25 %). We describe in detail the various age groups of pheasants after hatching and present graphically the overall results of coprological examinations, showing pathways of infection of C. baileyi and C. meleagridis during the full rearing seasons of 2013 and 2014. We found very similar mean proportions of Cryptosporidium-positive samples over the entire 2013 period in pheasantry (173 pooled samples tested, 25 positive, 14.5 %) and 2014 (238 samples tested, 43 positive, 18.1 %). All tests were verified as being Cryptosporidium positive in 9 from 219 (prevalence 4.1 %) and 4 from 168 (prevalence 2.4 %) post-mortem examinations. Significantly, C. baileyi was found more frequently in faeces, with positivities ranging from 11.1 to 100 % (4->16-week-old pheasants). Oocysts of C. meleagridis were detected at ages 6->15 weeks ranging from 7.1 to 100 % in faeces during the rearing seasons. The burdens of C. baileyi (7 of 14 and 10 of 16) and C. meleagridis (5 of 14 and 7 of 16) for each year, in monitored brooder houses, flight pens and spread across all open areas were recorded. Oocysts of C. baileyi and C. meleagridis obtained from this study, and Cryptosporidium galli (obtained in another aviary from 36-week-old pheasants), were sequenced, and we characterized the highly variable 60-kDa glycoprotein gene of C. meleagridis. These results highlight the real risk of transmission of Cryptosporidium to susceptible wild birds and other potential hosts after termination of rearing and release.
authors.affiliation: Department of Pathology and Parasitology, State Veterinary Institute Prague, Sídlištní 136/24, 165 03, Prague 6, Czech Republic. biola.biola@seznam.cz.|Department of Animal Science and Food Processing in the Tropics and Subtropics, Faculty of Tropical AgriSciences, Czech University of Life Sciences, Prague, Czech Republic. biola.biola@seznam.cz.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Máca
authors.affiliation: Department of Pathology and Parasitology, State Veterinary Institute Prague, Sídlištní 136/24, 165 03, Prague 6, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Pavlásek
mesh_terms: D000375:Aging; D000818:Animals; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D003458:Cryptosporidium / Q000235:genetics; D018153:Czech Republic / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D046094:Galliformes; D007422:Intestines / Q000469:parasitology; D033661:Oocysts; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*
keywords: C. baileyi; C. galli; Cryptosporidium meleagridis; Dynamics and transmission; GP60; Prevalence
references.citation: Aust Vet J. 1985 Aug;62(8):284-5
references.pmid: 4062742
references.citation: Folia Parasitol (Praha). 1989;36(3):287-8
references.pmid: 2583618
references.citation: Avian Pathol. 2007 Aug;36(4):275-8
references.pmid: 17620172
references.citation: Int J Parasitol. 2012 Oct;42(11):963-7
references.pmid: 23022616
references.citation: Vet Parasitol. 2012 Jul 6;187(3-4):534-7
references.pmid: 22377447
references.citation: J Clin Microbiol. 2014 Jul;52(7):2311-9
references.pmid: 24740082
references.citation: Appl Environ Microbiol. 2006 Dec;72(12):7548-53
references.pmid: 17028234
references.citation: Vet Rec. 1991 Oct 12;129(15):337-8
references.pmid: 1750176
references.citation: J Wildl Dis. 1983 Apr;19(2):152-3
references.pmid: 6887440
references.citation: Avian Dis. 1966 Nov;10(4):428-37
references.pmid: 6008334
references.citation: J Comp Pathol. 1955 Jul;65(3):262-6
references.pmid: 13242675
references.citation: Vet Parasitol. 1987 Dec;26(1-2):1-11
references.pmid: 2964117
references.citation: J Protozool. 1986 May;33(2):289-96
references.pmid: 3735157
references.citation: Vet Parasitol. 2015 Apr 30;209(3-4):164-8
references.pmid: 25814162
references.citation: Int J Parasitol. 2001 Mar;31(3):289-96
references.pmid: 11226456
references.citation: Vet Med (Praha). 1993;38(10):629-38
references.pmid: 8259642
references.citation: Int J Parasitol. 2014 Jul;44(8):515-21
references.pmid: 24727090
references.citation: Appl Environ Microbiol. 2005 Aug;71(8):4446-54
references.pmid: 16085835
references.citation: Vet Rec. 1986 Feb 22;118(8):211
references.pmid: 3716166
references.citation: Am Nat. 2003 Apr;161(4):657-71
references.pmid: 12778891
references.citation: J Helminthol. 2006 Sep;80(3):313-8
references.pmid: 16923277
references.citation: Appl Environ Microbiol. 2003 Jul;69(7):4302-7
references.pmid: 12839819