title: Evaluation of a fluorescent immunoassay rapid test (Sofia™) for detection of influenza A+B and RSV in a tertiary pediatric setting.
abstract: In response to the lack of sensitivity and reproducibility of previously marketed rapid antigen detection tests, a novel fluorescent immunoassay was recently developed. This new assay offers rapidity and automated reading. More characterization of this assay is needed. The aim of this study was to assess diagnostic performance of Sofia influenza A+B and respiratory syncytial virus (RSV) while compared to traditional viral cell culture. A total of 416 respiratory samples were analyzed prospectively with both methods in a tertiary pediatric center. Sensitivity and specificity of the Sofia™ test were 90.0% and 98.0% for influenza A, 90.9% and 98.9% for influenza B, and 87.7% and 94.7% for RSV compared to traditional cell culture. Overall, Sofia influenza A+B and RSV assays performed well in comparison to culture in a pediatric population.
authors.affiliation: Département de pédiatrie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada.
authors.forename: Pierre-Philippe
authors.initials: PP
authors.lastname: Piché-Renaud
authors.affiliation: Département de microbiologie et immunologie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Turcot
authors.affiliation: Département de pédiatrie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada.
authors.forename: Caroline
authors.initials: C
authors.lastname: Chartrand
authors.affiliation: Département de pédiatrie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada.
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Gravel
authors.affiliation: Département de microbiologie et immunologie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada.
authors.forename: Manon
authors.initials: M
authors.lastname: Labrecque
authors.affiliation: Département de microbiologie et immunologie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada.
authors.forename: Émilie
authors.initials: É
authors.lastname: Vallières
authors.affiliation: Département de microbiologie et immunologie, Centre Hospitalier Universitaire Ste-Justine, 3175 Chemin de la Côte-Sainte-Catherine, Montreal, H3T 1C4, QC, Canada. Electronic address: christian.renaud@umontreal.ca.
authors.forename: Christian
authors.initials: C
authors.lastname: Renaud
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D008297:Male; D011446:Prospective Studies; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D062606:Tertiary Care Centers; D013997:Time Factors
keywords: Influenza; Rapid antigen detection test; Respiratory syncytial virus; Respiratory viruses; Sofia; Viral culture