title: Ru(bpy)3(2+)/nanoporous silver-based electrochemiluminescence immunosensor for alpha fetoprotein enhanced by gold nanoparticles decorated black carbon intercalated reduced graphene oxide.
abstract: A highly sensitive sandwich-type electrochemiluminescence (ECL) immunosensor was proposed for the quantitative determination of alpha fetoprotein (AFP) using gold nanoparticles decorated black carbon intercalated reduced graphene oxide (Au-rGO@CB) as sensing platform and nanoporous silver (NPS) loaded Ru(bpy)3(2+) as labels. In this work, intercalation of CB inhibited the accumulation of rGO and Au-rGO@CB was firstly used to immobilize primary antibody (Ab1) in ECL system. NPS prepared by the dealloying of binary alloy has high pore volume and surface areas, which was used to load amount of secondary antibodies (Ab2) and Ru(bpy)3(2+), which could greatly enhance the ECL intensity. Under optimal conditions, the designed immunosensor exhibited wider linear range from 0.0001 to 30 ng/mL with a relative lower detection limit of 33 fg/mL for AFP detection. Overall, the designed immunosensor exhibited high sensitivity and selectivity, good repeatability and stability. This proposed method provided a potential application for clinical monitoring of AFP.
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Lv
authors.affiliation: School of Material Science and Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Hu
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010087:Oxides; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D000509:alpha-Fetoproteins
chemical_list: D010087:Oxides; D000509:alpha-Fetoproteins; D012834:Silver; D006046:Gold; D006108:Graphite
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