title: Perturbation of nucleo-cytoplasmic transport affects size of nucleus and nucleolus in human cells.
abstract: Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30-80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo-cytoplasmic transport causes the KR value to increase by 30-50% in immortalized cell lines. Nucleolus volumes are significantly increased in transformed cell lines and the attenuation of nucleo-cytoplasmic transport causes a significant increase in the nucleolus volume of immortalized cell lines. A cytosol and nuclear fraction swapping experiment emphasizes the potential role of unknown cytosolic factors in nuclear and nucleolar size regulation.
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Abira
authors.initials: A
authors.lastname: Ganguly
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Chumki
authors.initials: C
authors.lastname: Bhattacharjee
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Madhura
authors.initials: M
authors.lastname: Bhave
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Vaishali
authors.initials: V
authors.lastname: Kailaje
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Bhawik K
authors.initials: BK
authors.lastname: Jain
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Isha
authors.initials: I
authors.lastname: Sengupta
authors.affiliation: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science Bangalore, India.
authors.forename: Annapoorni
authors.initials: A
authors.lastname: Rangarajan
authors.affiliation: Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Bhattacharyya
mesh_terms: D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism*; D053145:Cell Nucleus Size; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D028961:beta Karyopherins / Q000235:genetics; D020931:ran GTP-Binding Protein / Q000235:genetics
chemical_list: D028961:beta Karyopherins; D020931:ran GTP-Binding Protein
keywords: Ran; cancer; importin beta; karyoplasmic ratio; nuclear transport; nucleolus size; nucleus size; organelle size