title: The modified corneocyte surface area measurement as an index of epidermal barrier properties: inverse correlation with transepidermal water loss.
abstract: BACKGROUND
  Corneocyte surface area (CSA) is as established parameter for skin barrier function in humans. Measurement of canine CSA has been previously reported but has not been validated.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the validity of CSA as a barrier function parameter in dogs.
  
  
  METHODS
  Six clinically normal beagle dogs.
  
  
  METHODS
  CSA was measured and compared with transepidermal water loss (TEWL) using sequential tape stripping of the stratum corneum as a model of acute barrier disruption. Then, CSA and TEWL were measured at four anatomical sites (groin, lower back, nasal bridge and pinna). The correlation between the two indices was also evaluated.
  
  
  RESULTS
  From the results of sequential tape stripping, CSA values gradually decreased with increasing number of tape strippings. The CSA values were inversely correlated with the TEWL ones. The two indices at different sites were variable and were strongly correlated.
  
  
  CONCLUSIONS
  Canine CSA was demonstrated to be a useful parameter for the canine skin barrier function. The results from the anatomical sites imply that the cephalic sites (nasal bridge and pinna) were lower than others in skin barrier function.
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Momota
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Shimada
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Noguchi
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Saito
authors.affiliation: Laboratory of Biomolecular Chemistry, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nozawa
authors.affiliation: Veterinary Medical Teaching Hospital, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Niina
authors.affiliation: Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Tani
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
mesh_terms: D000818:Animals; D004285:Dogs / Q000502:physiology*; D004817:Epidermis / Q000502:physiology*; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena; D014870:Water Loss, Insensible / Q000502:physiology*