title: The VP3 structural protein of foot-and-mouth disease virus inhibits the IFN-β signaling pathway.
abstract: Foot-and-mouth disease is a frequently occurring disease of cloven-hoofed animals that is caused by infection with the foot-and-mouth virus (FMDV). FMDV circumvents the type-I IFN response by expressing proteins that antagonize cellular innate immunity, such as leader protease and 3C protease. We identified the FMDV structural protein VP3 as a negative regulator of the virus-triggered IFN-β signaling pathway. Expression of FMDV VP3 inhibited the Sendai virus-triggered activation of IFN regulatory factor-3 and the expression of retinoic acid-inducible gene-I/melanoma differentiation-associated protein-5. Transient transfection and coimmunoprecipitation confirmed that the structural protein VP3 interacts with virus-induced signaling adapter (VISA), which is dependent on the C-terminal aa 111-220 of VP3. In addition, we found that FMDV VP3 inhibits the expression of VISA by disrupting its mRNA. Taken together, our findings reveal a novel strategy used by the structural VP3 protein of FMDV to evade host innate immunity.-Li, D., Yang, W., Yang, F., Liu, H., Zhu, Z., Lian, K., Lei, C., Li, S., Liu, X., Zheng, H., Shu, H. The VP3 structural protein of foot-and-mouth disease virus inhibits the IFN-β signaling pathway.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Wenping
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Huanan
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Zixiang
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Kaiqi
authors.initials: K
authors.lastname: Lian
authors.affiliation: Collaborative Innovation Center for Viral Immunology, Medical Research Institute, Wuhan University, Wuhan, China.
authors.forename: Caoqi
authors.initials: C
authors.lastname: Lei
authors.affiliation: Collaborative Innovation Center for Viral Immunology, Medical Research Institute, Wuhan University, Wuhan, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and haixuezheng@163.com.
authors.forename: Haixue
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Collaborative Innovation Center for Viral Immunology, Medical Research Institute, Wuhan University, Wuhan, China. shuh@whu.edu.cn.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Shu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate; D016899:Interferon-beta / Q000502:physiology*; D011485:Protein Binding; D000072417:Protein Domains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013552:Swine
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D036022:Capsid Proteins; C505371:MAVS protein, human; D012333:RNA, Messenger; C442460:VP3 protein, Foot-and-mouth disease virus; D016899:Interferon-beta
keywords: Aphthovirus; innate immunity; pathogen