title: [Expression and clinical significance of ING4 and HIF-1 alpha in brain astrocytoma].
abstract: OBJECTIVE
  To study the mRNA expression level of growth inhibition factor 4 (ING4) and hypoxia inducing factor 1 alpha (HIF-1 alpha), and their relationship with tumor malignant degree or the pathology classification in human brain astrocytoma.
  
  
  METHODS
  The mRNA expression levels of ING4 and HIF-1 alpha were detected by RT-PCR method in 45 cases of grade I-IV human brain astrocytoma and 11 cases of control brain tissues from January 2009 to June 2010 in the Second Affiliated Hospital of Zhengzhou University, and their correlation was also analyzed.
  
  
  RESULTS
  In the non-tumor brain tissue, the expression level of ING4 mRNA was 1.19 ± 0.22, while they were 0.91 ± 0.19, 0.74 ± 0.28, 0.54 ± 0.33 and 0.22 ± 0.19 in I-IV grade astrocytoma, respectively. Compared with the non-tumor control, the mRNA expression level of ING4 gene decreased significantly in the astrocytoma (P<0.05). And the expression of ING4 gradually reduced with the increase of the pathological classification of the astrocytoma.In the non-tumor brain tissue, the expression level of HIF-1 alpha mRNA was 0.26 ± 0.16, and they were 0.34 ± 0.19, 0.50 ± 0.23, 0.96 ± 0.15 and 1.04 ± 0.15 in I-IV grade astrocytoma, respectively.For HIF-1 alpha gene, the mRNA expression level increased significantly in the astrocytoma. Meanwhile, the expression gradually increased with the increase of the pathological classification of the astrocytoma (P<0.05). The mRNA expression showed a negative correlation between ING4 and HIF-1 alpha with the increase of the tumor malignant degree.
  
  
  CONCLUSIONS
  The ING4 and HIF-1 alpha genes play a role in the tumorigenesis and development of human brain astrocytoma, and closely associate with the malignant degree of astrocytoma.
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China.
authors.forename: Shupeng
authors.initials: S
authors.lastname: Zhao
authors.forename: Cailing
authors.initials: C
authors.lastname: Jin
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhao
authors.forename: Baozhe
authors.initials: B
authors.lastname: Jin
authors.forename: Lei
authors.initials: L
authors.lastname: Hui
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhou
authors.forename: Guangming
authors.initials: G
authors.lastname: Niu
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China, Email: tao2000zz@163.com.
authors.forename: Shengzhong
authors.initials: S
authors.lastname: Tao
mesh_terms: D001254:Astrocytoma; D001932:Brain Neoplasms; D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins
chemical_list: D018797:Cell Cycle Proteins; C497442:HIF1A protein, human; D018398:Homeodomain Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C483230:ING4 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins