title: Preparation of albumin based nanoparticles for delivery of fisetin and evaluation of its cytotoxic activity.
abstract: Fisetin is a well known flavonoid that shows several properties such as antioxidant, antiviral and anticancer activities. Its use in the pharmaceutical field is limited due to its poor aqueous solubility which results in poor bioavailability and poor permeability. The aim of our present study is to prepare fisetin loaded human serum albumin nanoparticles to improve its bioavailability. The nanoparticles were prepared by a desolvation method and characterized by spectroscopic and microscopic techniques. The particles were smooth and spherical in nature with an average size of 220 ± 8 nm. The encapsulation efficiency was found to be 84%. The in vitro release profile showed a biphasic pattern and the release rate increases with increase in ionic strength of solution. We have also confirmed the antioxidant activity of the prepared nanoparticles by a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Further its anticancer activity was evaluated using MCF-7 breast cancer cell lines. Our findings suggest that fisetin loaded HSA nanoparticles could be used to transfer fisetin to target areas under specific conditions and thus may find use as a delivery vehicle for the flavonoid.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Singha Roy
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Susmitnarayan
authors.initials: S
authors.lastname: Chaudhury
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Saikat Kumar
authors.initials: SK
authors.lastname: Jana
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Koel
authors.initials: K
authors.lastname: Chaudhury
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address: swagata@chem.iitkgp.ernet.in.
authors.forename: Swagata
authors.initials: S
authors.lastname: Dasgupta
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity*; D065546:Drug Liberation; D005419:Flavonoids / Q000737:chemistry*; D044948:Flavonols; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity*; D010316:Particle Size; D010851:Picrates / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D004337:Drug Carriers; D005419:Flavonoids; D044948:Flavonols; D010851:Picrates; D012709:Serum Albumin; C004931:1,1-diphenyl-2-picrylhydrazyl; C017875:fisetin
keywords: Cytotoxic; Hemolytic; Nanoparticles