title: [The effects and mechanisms of high glucose on the phenotype transformation of rat vascular smooth muscle cells].
abstract: OBJECTIVE
  To investigate the effects and mechanisms of high glucose on the phenotype transformation of rat vascular smooth muscle cells (VSMCs).
  
  
  METHODS
  VSMCs ere isolated from rat thoracic aorta and the 3rd-5th VSMCs were incubated with normal glucose (5.5 mmol/L), high glucose (25 mmol/L), or high glucose (25 mmol/L) + P38 inhibitor (25 mmol/L +SB203580) for another 24 hours. Then the gene expression of osteopontin (OPN), alpha smooth-actin (alpha-SMA), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9(MMP-9) were assayed by real time RT-PCR, the protein expression of P38 were assayed by Western blot.
  
  
  RESULTS
  (1) High glucose promoted the phenotype transformation of VSMCs and up-regulated the expression of MMP-2 and MMP-9. (2) High glucose promoted the phosphorylation of P38. (3) SB203580, the inhibitor of P38/MAPK signal pathway, inhibited the effects of high glucose on phenotype transformation and expression of MMP-2 and MMP-9.
  
  
  CONCLUSIONS
  High glucose may promote phenotype transformation of VSMCs via the signal pathway of P38/MAPK.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Chu
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D015153:Blotting, Western; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects; D053495:Osteopontin / Q000378:metabolism; D010641:Phenotype; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D005947:Glucose; D007093:Imidazoles; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D053495:Osteopontin; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; C506897:Spp1 protein, rat; C498862:Acta2 protein, rat; C522363:Mmp2 protein, rat; C572337:Mmp9 protein, rat; C093642:SB 203580