title: Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo.
abstract: Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D-hep cells, Hepa1-6 cells treated with DMSO for 7 days, could restore to the higher proliferation rate in DMSO-free medium, but alteration of gene expression profile was irreversible. Interestingly, tumors from D-hep cells, not Hepa1-6 cells, regressed in wild-type C57BL/6 mice whereas D-hep cells exhibited similar tumorigenesis as Hep1-6 cells in immunodeficient mice. As expected, additional Hepa1-6 cells failed to form tumors in the D-hep-C57 mice in which D-hep cells were eliminated. Further research confirmed that D-hep-C57 mice established anti-tumor immunity against Hepa1-6 cells. Our research proposed viable tumor cells with altered biological features by DMSO-treatment could induce anti-tumor immunity in vivo.
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.|Department of Anesthesiology, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Training Department, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Minjun
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Xinlu
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Clinic Medicine, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Pan
authors.affiliation: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, P.R. China.|Center for Stem Cell and Medicine, The Graduate School, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology; D007167:Immunotherapy / Q000379:methods; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008819:Mice, Nude; D016513:Mice, SCID; D055611:Natural Killer T-Cells / Q000187:drug effects / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*
chemical_list: D004121:Dimethyl Sulfoxide
keywords: DMSO; anti-tumor immunity; cancer immunotherapy
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