title: miR-203 Functions as a Tumor Suppressor by Inhibiting Epithelial to Mesenchymal Transition in Ovarian Cancer.
abstract: OBJECTIVE
  Ovarian cancer is a gynecological malignancy that has a high mortality rate in women due to metastatic progression and recurrence. miRNAs are small, endogenous, noncoding RNAs that function as tumor suppressors or oncogenes in various human cancers by selectively suppressing the expression of target genes. The objective of this study is to investigate the role of miR-203 in ovarian cancer.
  
  
  METHODS
  miR-203 was expressed in ovarian cancer SKOV3 and OVCAR3 cells using lentiviral vector and cell proliferation, migration, invasion were examined using MTT, transwell and Matrigel assays, respectively. Tumor growth was examined using Xenograft mouse model.
  
  
  RESULTS
  miR-203 expression was downregulated, whereas expression of its target gene Snai2 was upregulated in human ovarian serous carcinoma tissue as compared to normal ovaries. In addition, high miR-203 expression was associated with long-term survival rate of ovarian cancer patients. miR-203 overexpression inhibited cell proliferation, migration, and invasion of SKOV3 and OVCAR3 ovarian cancer cells. Furthermore, miR-203 overexpression inhibited the epithelial to mesenchymal transition (EMT) in ovarian cancer cells. Silencing Snai2 with lentiviral short hairpin (sh) RNA mimics miR-203-mediated inhibition of EMT and tumor cell invasion. Xenografts of miR-203-overexpressing ovarian cancer cells in immunodeficient mice exhibited a significantly reduced tumor growth.
  
  
  CONCLUSIONS
  miR-203 functions as a tumor suppressor by down regulating Snai2 in ovarian cancer.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA; The Third Affiliated Hospital, Zhengzhou University, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Zhao
authors.affiliation: The Third Affiliated Hospital, Zhengzhou University, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA; Southern Medical University, Guangzhou, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Chuanhe
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Dudas
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Ziyun
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA; Southern Medical University, Guangzhou, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Harbin Medical University, P. R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, USA.
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Szabo
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, USA.
authors.forename: Sue-Chin
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Sims
authors.affiliation: Southern Medical University, Guangzhou, China.
authors.forename: Weiwang
authors.initials: W
authors.lastname: Gu
authors.affiliation: West Cancer Center, Memphis, TN, USA.
authors.forename: Todd
authors.initials: T
authors.lastname: Tillmanns
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Pfeffer
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, USA.
authors.forename: Gabor
authors.initials: G
authors.lastname: Tigyi
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, USA; Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Junming
authors.initials: J
authors.lastname: Yue
keywords: Epithelial to mesenchymal transition; Ovarian cancer; miR-203
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