title: Food intake inhibition in rainbow trout induced by activation of serotonin 5-HT2C receptors is associated with increases in POMC, CART and CRF mRNA abundance in hypothalamus.
abstract: In rainbow trout, the food intake inhibition induced by serotonin occurs through 5-HT2C and 5-HT1A receptors, though the mechanisms involved are still unknown. Therefore, we assessed if a direct stimulation of 5-HT2C and 5-HT1A serotonin receptors (resulting in decreased food intake in rainbow trout), affects gene expression of neuropeptides involved in the control of food intake, such as pro-opiomelanocortin (POMC), cocaine- and amphetamine-regulated transcript (CART), corticotrophin releasing factor (CRF), and agouti-related peptide (AgRP). In a first set of experiments, the injection of the 5-HT2C receptor agonists MK212 (60 μg kg(-1) icv) and WAY 161503 (1 mg kg(-1) ip), and of the 5-HT1A receptor agonist 8-OH-DPAT (1 mg kg(-1) ip and 30 μg kg(-1) icv) induced food intake inhibition. In a second set of experiments, we observed that the injection of MK212 or WAY 161503 (1 and 3 mg kg(-1)) significantly increased hypothalamic POMC mRNA abundance. CART mRNA abundance in hypothalamus was enhanced by treatment with MK212 and unaffected by WAY 161503. The administration of the 5-HT1A receptor agonist 8-OH-DPAT did not induce any significant variation in the hypothalamic POMC or CART mRNA levels. CRF mRNA abundance was only affected by MK212 that increased hypothalamic values. Finally, hypothalamic AgRP mRNA abundance was only evaluated with the agonist 5-HT2C MK212 resulting in no significant effects. The results show that the reduction in food intake mediated by 5-HT2C receptors is associated with increases in hypothalamic POMC, CART and CRF mRNA abundance.
authors.affiliation: Laboratorio de Fisiología Animal (Instituto de Acuicultura), Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
authors.forename: Jorge J
authors.initials: JJ
authors.lastname: Pérez-Maceira
authors.affiliation: Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310, Vigo, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Otero-Rodiño
authors.affiliation: Laboratorio de Fisiología Animal (Instituto de Acuicultura), Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Mancebo
authors.affiliation: Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310, Vigo, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Soengas
authors.affiliation: Laboratorio de Fisiología Animal (Instituto de Acuicultura), Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain. manuelalejo.aldegunde@usc.es.
authors.forename: Manuel
authors.initials: M
authors.lastname: Aldegunde
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D054369:Agouti-Related Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D004435:Eating / Q000502:physiology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000502:physiology*; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D011719:Pyrazines / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism; D044405:Receptor, Serotonin, 5-HT2C / Q000378:metabolism*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: C502777:8,9-dichloro-2,3,4,4a-tetrahydro-1H-pyrazino(1,2-a)quinoxalin-5(6H)-one; D054369:Agouti-Related Protein; D029941:Fish Proteins; D009419:Nerve Tissue Proteins; D011719:Pyrazines; D011810:Quinoxalines; D012333:RNA, Messenger; D044405:Receptor, Serotonin, 5-HT2C; D017366:Serotonin Receptor Agonists; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D044282:Receptor, Serotonin, 5-HT1A; C014896:6-chloro-2-(1-piperazinyl)pyrazine; D011333:Pro-Opiomelanocortin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D003346:Corticotropin-Releasing Hormone
keywords: Food intake; Hypothalamus; Neuropeptides; Rainbow trout; Serotonin receptors
references.citation: Physiol Behav. 1993 Mar;53(3):517-20
references.pmid: 8451317
references.citation: Rev Esp Fisiol. 1995 Jun;51(2):71-5
references.pmid: 8532953
references.citation: Neuroreport. 2002 May 24;13(7):945-51
references.pmid: 12004196
references.citation: Am J Physiol. 1999 Sep;277(3 Pt 2):R802-11
references.pmid: 10484498
references.citation: Gen Comp Endocrinol. 2005 May 15;142(1-2):3-19
references.pmid: 15862543
references.citation: Peptides. 1998;19(3):505-11
references.pmid: 9533638
references.citation: Regul Pept. 2003 Sep 15;115(2):101-13
references.pmid: 12972325
references.citation: Front Endocrinol (Lausanne). 2012 Dec 27;3:169
references.pmid: 23293630
references.citation: Ann N Y Acad Sci. 2009 Apr;1163:241-50
references.pmid: 19456345
references.citation: Peptides. 2008 Nov;29(11):1930-6
references.pmid: 18656512
references.citation: Am J Physiol Regul Integr Comp Physiol. 2000 Jul;279(1):R47-52
references.pmid: 10896863
references.citation: Front Neurosci. 2013 May 30;7:91
references.pmid: 23754974
references.citation: Eur J Pharmacol. 2011 Jun 11;660(1):53-60
references.pmid: 21208603
references.citation: J Exp Biol. 2012 Sep 1;215(Pt 17):2969-79
references.pmid: 22875766
references.citation: J Endocrinol. 2008 Mar;196 (3):637-48
references.pmid: 18310459
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2009 May;153(1):8-12
references.pmid: 19087889
references.citation: Brain Res. 1991 Aug 2;555(2):245-50
references.pmid: 1933336
references.citation: J Chem Neuroanat. 2010 Jul;39(4):235-41
references.pmid: 20080175
references.citation: PLoS One. 2013;8(2):e55217
references.pmid: 23460782
references.citation: J Am Board Fam Pract. 1999 Mar-Apr;12(2):137-42
references.pmid: 10220237
references.citation: Neuron. 2008 Nov 26;60(4):582-9
references.pmid: 19038216
references.citation: Pharmacol Biochem Behav. 2011 Apr;98(2):311-5
references.pmid: 21281662
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2014 Apr;161:1-6
references.pmid: 24365333
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2011 Jan;158(1):79-86
references.pmid: 20840870
references.citation: Cell Metab. 2007 Nov;6(5):352-61
references.pmid: 17983581
references.citation: J Endocrinol. 2014 Jun;221(3):441-55
references.pmid: 24741070
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: PLoS One. 2013 Jun 06;8(6):e65317
references.pmid: 23762342
references.citation: Neuropeptides. 2014 Oct;48(5):313-8
references.pmid: 25130282
references.citation: Endocrinology. 2008 Mar;149(3):1323-8
references.pmid: 18039773
references.citation: J Neurosci. 2003 Aug 27;23(21):7863-72
references.pmid: 12944516
references.citation: Appetite. 2003 Dec;41(3):335-7
references.pmid: 14637334
references.citation: Recent Prog Horm Res. 1996;51:287-317; discussion 318
references.pmid: 8701084
references.citation: Nat Neurosci. 2012 Oct;15(10):1343-9
references.pmid: 23007189
references.citation: Cell Metab. 2007 Nov;6(5):398-405
references.pmid: 17983585
references.citation: Front Neurosci. 2013 Oct 29;7:196
references.pmid: 24194695
references.citation: J Endocrinol. 2006 Feb;188(2):355-63
references.pmid: 16461561
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2013 Sep;166(1):186-90
references.pmid: 23752124
references.citation: Pharmacol Biochem Behav. 2002 Apr;71(4):709-16
references.pmid: 11888562
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2000 Aug;126(4):415-34
references.pmid: 10989336
references.citation: Brain Res. 2006 May 4;1087(1):83-6
references.pmid: 16626640
references.citation: Regul Pept. 2003 Sep 15;115(2):73-80
references.pmid: 12972322
references.citation: J Exp Biol. 2010 Nov 15;213(Pt 22):3858-65
references.pmid: 21037065
references.citation: Nutr Metab Cardiovasc Dis. 2008 Feb;18(2):158-68
references.pmid: 18061414
references.citation: Gen Comp Endocrinol. 2009 Jan 15;160(2):134-8
references.pmid: 19027741
references.citation: Physiol Behav. 2008 Oct 20;95(3):484-91
references.pmid: 18694771
references.citation: Mol Pharmacol. 2007 Oct;72(4):885-96
references.pmid: 17622577
references.citation: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2002 Jul;188(6):479-84
references.pmid: 12122466
references.citation: Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):2036-40
references.pmid: 9050900
references.citation: Prog Mol Biol Transl Sci. 2011;98:235-92
references.pmid: 21199774
references.citation: J Exp Biol. 2010 Jun 1;213(11):1813-21
references.pmid: 20472767
references.citation: Gen Comp Endocrinol. 2013 Oct 1;192:246-55
references.pmid: 24013027
references.citation: Regul Pept. 2009 Jun 5;155(1-3):99-104
references.pmid: 19344674
references.citation: Neuroendocrinology. 2001 Apr;73(4):248-60
references.pmid: 11340339
references.citation: Front Neurosci. 2013 Mar 27;7:36
references.pmid: 23543912
references.citation: Science. 2002 Jul 26;297(5581):609-11
references.pmid: 12142539
references.citation: Eur J Pharmacol. 2000 Nov 24;408(3):273-6
references.pmid: 11090644
references.citation: J Physiol. 2009 Jan 15;587(1):49-60
references.pmid: 19029184
references.citation: Behav Brain Res. 1996;73(1-2):37-42
references.pmid: 8788474
references.citation: Neuroscience. 1996 May;72(2):557-66
references.pmid: 8737424
references.citation: Rev Assoc Med Bras (1992). 2011 Jan-Feb;57(1):74-7
references.pmid: 21390463
references.citation: Neuron. 2006 Jul 20;51(2):239-49
references.pmid: 16846858
references.citation: Physiol Behav. 2010 Apr 19;99(5):632-7
references.pmid: 20138904
references.citation: Am J Physiol Regul Integr Comp Physiol. 2012 Jun;302(11):R1340-50
references.pmid: 22496361
references.citation: Endocrinology. 1998 Oct;139(10):4428-31
references.pmid: 9751529
references.citation: Methods Find Exp Clin Pharmacol. 2007 May;29(4):269-72
references.pmid: 17609739
references.citation: Peptides. 2006 Apr;27(4):719-27
references.pmid: 16253390
references.citation: J Pharm Pharmacol. 1992 Aug;44(8):696-8
references.pmid: 1359101
references.citation: Br J Pharmacol. 1998 Aug;124(7):1507-15
references.pmid: 9723965
references.citation: Diabetes. 2001 Feb;50(2):248-54
references.pmid: 11272133
references.citation: Gen Comp Endocrinol. 2014 Sep 1;205:36-48
references.pmid: 24530522