title: Analysis of lignans in Magnoliae Flos by turbulent flow chromatography with online solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry.
abstract: In this study, a method coupling turbulent flow chromatography with online solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry was developed for analyzing the lignans in Magnoliae Flos. By the online pretreatment of turbulent flow chromatography solid-phase extraction, the impurities removal and analytes concentration were automatically processed, and the lignans were separated rapidly and well. Seven lignans of Magnoliae Flos including epieudesmin, magnolin, 1-irioresinol-B-dimethyl ether, epi-magnolin, fargesin aschantin, and demethoxyaschantin were identified by comparing their retention behavior, UV spectra, and mass spectra with those of reference substances or literature data. The developed method was validated, and the good results showed that the method was not only automatic and rapid, but also accurate and reliable. The turbulent flow chromatography with online solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry method holds a high potential to become an effective method for the quality control of lignans in Magnoliae Flos and a useful tool for the analysis of other complex mixtures.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.
authors.forename: Fenyun
authors.initials: F
authors.lastname: Song
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, PR China.|Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, PR China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.|School of Public Health, Guangdong Pharmaceutical University, Guangzhou, PR China.
authors.forename: Fuhai
authors.initials: F
authors.lastname: Wu
mesh_terms: D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D017705:Lignans / Q000032:analysis*; D031566:Magnolia / Q000737:chemistry*; D008968:Molecular Conformation; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D017705:Lignans
keywords: Lignans; Magnoliae Flos; Solid-phase extraction; Turbulent flow chromatography