title: Novel Imaging Methods for Analysis of Tissue Resident Cells in HIV/SIV.
abstract: The use of advanced tissue-imaging methodologies has greatly facilitated the study of molecular mechanisms and cellular interactions in humans and animal models of disease. Particularly, in HIV research, there is an ever-increasing demand for a comprehensive analysis of immune cell dynamics at tissue level stemming from the need to advance our understanding of those interactions that regulate the generation of adaptive antigen-specific immune responses. The latter is critical for the development of vaccines to elicit broadly neutralizing antibodies as well as for the discovery of novel targets for immuno-therapies to strengthen the cytolytic arm of the immune system at local level. In this review, we focus on current and emerging imaging technologies, discuss their strengths and limitations, and examine how such technologies can inform the development of new treatments and vaccination strategies. We also present some perspective on the future of the technology development.
authors.affiliation: Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, 33136-1013, USA.
authors.forename: Eirini
authors.initials: E
authors.lastname: Moysi
authors.affiliation: AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.
authors.forename: Jacob D
authors.initials: JD
authors.lastname: Estes
authors.affiliation: Immunology Laboratory, Vaccine Research Center, NIAID, National Institutes of Health, Building 40, 40 Convent Drive, Bethesda, MD, 20892-3005, USA. petrovasc@mail.nih.gov.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Petrovas
mesh_terms: D000818:Animals; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D018613:Microscopy, Confocal / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology*; D015302:Simian Immunodeficiency Virus; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: HIV; HIV pathogenesis; Imaging; Review; SIV; Tissues
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