title: Differential Modulation of Lipopolysaccharide-Induced Inflammatory Cytokine Production by and Antioxidant Activity of Fomentariol in RAW264.7 Cells.
abstract: Medicinal mushrooms have been used worldwide to treat cancer and modulate the immune system. Over the last several years, there has been increasing interest in isolating bioactive compounds from medicinal mushrooms and evaluating their health beneficial effects. Fomes fomentarius is used in traditional oriental medicine and is known to possess antioxidant, anti-inflammatory, antidiabetic, and antitumor effects. In the present study, we isolated fomentariol from Fomes fomentarius and investigated its anti-inflammatory effect in murine macrophages (RAW264.7 cells) stimulated with lipopolysaccharides. Fomentariol inhibited the production of nitric oxide and intracellular reactive oxygen species triggered by lipopolysaccharides. Interestingly, fomentariol differentially regulated cytokine production triggered by lipopolysaccharides. Fomentariol effectively suppressed the production of interleukin-1β and interleukin-6 but not tumor necrosis factor-α. The inhibitory effect of fomentariol against nitric oxide, interleukin-1β, and interleukin-6 production was possibly mediated by downregulation of the extracellular signal-regulated kinase signaling pathway. Taken together, our results suggest that fomentariol differentially modulated inflammatory responses triggered by lipopolysaccharides in macrophages and is one of the bioactive compounds that mediate the physiological effects of Fomes fomentarius.
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Yi
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Myeong-Seok
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Sang-Myeong
authors.initials: SM
authors.lastname: Lee
keywords: ERK signaling pathway; Fomentariol; Fomes fomentarius; LPS-induced inflammatory responses; RAW264.7 cells
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