title: Macrophageal infiltration and microvessel density in laryngeal carcinoma: study of 52 cases.
abstract: Angiogenesis is one of the six originally constituted hallmarks of cancer that has been extensively studied in the last two decades. The aim of our study is to assess the microvessel and macrophageal density in laryngeal carcinoma and its clinicopathological correlations. We immunohistochemically assessed microvessel density (CD34) and macrophage count (CD68) using microarray techniques and then looked for clinicopathological correlations. The mean micro-vessel density in the study group was 14.27 ± 12.92 vessels in a ×200 field with a mean macrophageal infiltration density of 5.19 ± 4.32. Median microvessel density was significantly higher in patients with metastasis than in patients without metastasis. Additionally, linear regression established that macrophageal infiltration density could predict microvessel density in laryngeal carcinoma. We found no association between either factor and recurrence rate or other clinical characteristics. Our study adds additional data to a problem that has been widely studied during the last two decades, even if controversies in this area still remain.
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: T M
authors.initials: TM
authors.lastname: Popov
authors.affiliation: Department of Pathology, Medical University of Sofia, Bulgaria.
authors.forename: T
authors.initials: T
authors.lastname: Dikov
authors.affiliation: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Bulgaria;
authors.forename: T E
authors.initials: TE
authors.lastname: Goranova
authors.affiliation: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Bulgaria;
authors.forename: G
authors.initials: G
authors.lastname: Stancheva
authors.affiliation: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Bulgaria;
authors.forename: R P
authors.initials: RP
authors.lastname: Kaneva
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: S
authors.initials: S
authors.lastname: Todorov
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: O
authors.initials: O
authors.lastname: Stoyanov
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: J
authors.initials: J
authors.lastname: Rangachev
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: T
authors.initials: T
authors.lastname: Marinov
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: D P
authors.initials: DP
authors.lastname: Popova
authors.affiliation: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Bulgaria;
authors.forename: V I
authors.initials: VI
authors.lastname: Mitev
authors.affiliation: Department of ENT, Medical University of Sofia, Bulgaria;
authors.forename: D
authors.initials: D
authors.lastname: Konov
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000098:blood supply*; D055806:Microvessels; D009364:Neoplasm Recurrence, Local; D009389:Neovascularization, Pathologic; D011379:Prognosis
keywords: CD31; CD68; Laryngeal carcinoma; Macrophage count; Micro-vessel density
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