title: Isolation, complete genome sequencing, and phylogenetic analysis of the first Chuzan virus in China.
abstract: A Chuzan virus (CHUV), defined as GX871 here, was isolated from blood from a sentinel cattle firstly in China, and its full-length genome was sequenced in this study. The GX871 genome included 10 segments and 18914 bp, one base fewer than the CHUV prototype strain K-47 due to a one-base deletion in the 5' non-coding region of segment 8. A frameshift mutation was detected in a short coding region (1010-1026 nt) corresponding to the VP1 protein; this frameshift resulted in a five-amino acid mutation from 336CVLSY340 to 336YGAKL340. In addition, there were a one-base deletion at 1713 nt and a one-base insertion at 1682 nt in the 3' non-coding region of segment 5. Based on phylogenetic analysis of the deduced VP2 amino acid sequences, Palyam serogroup viruses were classified into three groups. The Chinese CHUV isolate GX871 was categorized into the same group as CHUV prototype strain K-47. The phylogenetic tree was divided into three clusters according to the geographical distribution of the partial nucleotide sequences of VP7, and this arrangement might define the geographical gene pool of CHUV.
authors.affiliation: Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China. wfangemail@aliyun.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Guangxi Veterinary Research Institute, Nanning, People's Republic of China. spirit1899@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China. changjitao_2005@126.com.
authors.forename: Jitao
authors.initials: J
authors.lastname: Chang
authors.affiliation: Guangxi Veterinary Research Institute, Nanning, People's Republic of China. 2317104460@qq.com.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Guangxi Veterinary Research Institute, Nanning, People's Republic of China. 0134130120@163.com.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Qin
authors.affiliation: Guangxi Veterinary Research Institute, Nanning, People's Republic of China. wu_jm20@163.com.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China. liyu@hvri.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D002417:Cattle / Q000821:virology*; D002681:China; D016679:Genome, Viral; D030081:Palyam Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny
keywords: Chuzan virus; Complete genome; Isolation in China; Phylogenetic analysis
references.citation: J Gen Virol. 1999 Jan;80 ( Pt 1):205-8
references.pmid: 9934703
references.citation: Virology. 1989 Jun;170(2):561-5
references.pmid: 2543130
references.citation: J Vet Med Sci. 2015 Feb;77(2):247-50
references.pmid: 25342637
references.citation: J Virol Methods. 2007 Aug;143(2):132-9
references.pmid: 17433453
references.citation: Nihon Juigaku Zasshi. 1990 Aug;52(4):689-94
references.pmid: 2167994
references.citation: Aust Vet J. 1994 Feb;71(2):57
references.pmid: 8166617
references.citation: PLoS Pathog. 2008 Sep 26;4(9):e1000163
references.pmid: 18818738
references.citation: J Clin Microbiol. 2004 Oct;42(10):4610-4
references.pmid: 15472317
references.citation: J Vet Sci. 2007 Mar;8(1):45-9
references.pmid: 17322773
references.citation: Am J Vet Res. 1988 Dec;49(12):2022-5
references.pmid: 3239836
references.citation: J Gen Virol. 2014 Feb;95(Pt 2):434-41
references.pmid: 24189621
references.citation: J Gen Virol. 2009 Jun;90(Pt 6):1423-32
references.pmid: 19264638
references.citation: J Gen Virol. 1999 Apr;80 ( Pt 4):937-41
references.pmid: 10211963
references.citation: Nihon Juigaku Zasshi. 1988 Jun;50(3):632-7
references.pmid: 2850386