title: Preparation of molecularly imprinted polymers using theanine as dummy template and its application as SPE sorbent for the determination of eighteen amino acids in tobacco.
abstract: In this paper, a novel dummy template molecularly imprinted polymer (DMIP) based on a vinyl-SiO2 microspheres surface for the simultaneous selective recognition and enrichment of 18 amino acids was prepared via a surface molecular imprinting technique using theanine as a dummy template. Compared to the imprinted polymers prepared using traditional polymerization techniques, the obtained DMIPs exhibited a regular spherical shape and were relatively monodisperse. The maximal sorption capacity (Qmax) of the resulting DMIPs for the 18 amino acids was up to 1444.3 mg g(-1). A kinetic binding study showed that the sorption capacity reached 85.40% of Qmax in 25 min and sorption equilibrium at 30 min. The imprint factors of the sorbents ranged from 2.86 to 6.9 for the 18 amino acids, which indicated that the DMIP sorbents have high selectivity. An HPLC-UV method for the simultaneous determination of 18 amino acids in tobacco and tobacco smoke was developed using the DMIPs as sorbents for solid phase extraction (SPE) in the sample pretreatment procedure. Under the optimum experimental conditions, the materials had enrichment factors of up to 200 for the amino acids, and the recoveries of the 18 amino acids in tobacco smoke were in the range from 79% to 104% with relative standard deviations of less than 7.4%. It indicated that the obtained DMIP sorbents could specifically recognize the amino acids from complicated samples.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, Sichuan 610066, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, Sichuan 610066, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, Sichuan 610066, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Tan
authors.affiliation: Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, Sichuan 610066, China.
authors.forename: Guanglin
authors.initials: G
authors.lastname: Feng
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address: liusm@ustc.edu.cn.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Liu
authors.affiliation: Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, Sichuan 610066, China. Electronic address: dycy@263.net.
authors.forename: Ya
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Research and Development Center, China Tobacco Anhui Industrial Corporation, Hefei, Anhui 230088, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005971:Glutamates / Q000737:chemistry*; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D005971:Glutamates; D011108:Polymers; D012822:Silicon Dioxide; C026166:theanine
keywords: Amino acids; Dummy template; Solid phase extraction; Surface molecularly imprinted polymers; Tobacco