title: Flavobacterium brevivitae sp. nov., isolated from river water.
abstract: A bacterial strain, designated TTM-43T, was isolated from a water sample taken from the Caohu River in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TTM-43T were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and surrounded by a thick capsule. The strain formed bright-yellow colonies. Growth occurred at 15-30 °C (optimum, 20 °C), at pH 5-9 (optimum, pH 6) and with 0-0.3% NaCl (optimum growth in the absence of added NaCl). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-43T belonged to the genus Flavobacterium and was most closely related to Flavobacterium terrae R2A1-13T, with 97.8% sequence similarity. Strain TTM-43T contained iso-C15:0 (24.9%), iso-C15:1 G (12.6%), summed feature 9 (iso-C17:1ω9c and/or 10-methyl C16:0; 12.2%), iso-C17:0 3-OH (10.9%), iso-C15:0 3-OH (7.3%) and iso-C16:0 (6.7%) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The genomic DNA G+C content of strain TTM-43T was 31 mol%. DNA-DNA relatedness of strain TTM-43T with respect to type strains of recognized species of the genus Flavobacterium was less than 70%. On the basis of phylogenetic inference and phenotypic data, strain TTM-43T should be assigned to a novel species, for which the name Flavobacterium brevivitae sp. nov. is presented. The type strain is TTM-43T (=BCRC 80913T=KCTC 42744T=LMG 29004T).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000737:chemistry; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6; D013095:Spermidine