title: Biodegradable Self-Assembled Nanoparticles of Galactose-Containing Amphiphilic Triblock Copolymers for Targeted Delivery of Paclitaxel to HepG2 Cells.
abstract: Biodegradable self-assembled polymeric nanoparticles (NPs) composed of poly(6-O-methacryloyl-D-galactopyranose)-b-poly(L-lactide)-b-poly(6-O-methacryloyl-D-galactopyranose) (PMAGP-b-PLA-b-PMAGP) are prepared as carriers for the hydrophobic anticancer drug paclitaxel (PTX), to achieve target delivery to hepatoma cells. PTX can be encapsulated by the NPs with various molar ratios of L-lactide (LA) and 6-O-methacryloyl-D-galactopyranose (MAGP) during the process of self-assembly, and the resulting NPs exhibit high drug loading efficacy and substantial stability in aqueous solution. The size, size distribution, and morphology of the NPs are characterized using a Zetasizer Nano ZS and transmission electron microscopy. The hemolysis assay and cell cytotoxicity assay indicate that the polymeric NPs are biocompatible and non-toxic. The cellular uptake assay demonstrates that the galactose-containing NPs can be selectively recognized and subsequently accumulate in HepG2 cells. All of these results demonstrate that galactose-containing polymeric NPs are potential carriers for hepatoma-targeted drug delivery and liver cancer therapy in clinical medicine.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
authors.forename: Tieshi
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Material Science and Engineering, Shandong Jiaotong University, Jinan, 250023, China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Tang
authors.affiliation: School of Medicine, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Jingtian
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Medicine, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
authors.forename: Meishan
authors.initials: M
authors.lastname: Pei
mesh_terms: D016503:Drug Delivery Systems; D005690:Galactose / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D011108:Polymers; D013501:Surface-Active Agents; D017239:Paclitaxel; D005690:Galactose
keywords: biodegradable copolymers; galactose-containing nanoparticles; liver cancer; specific recognition; targeted delivery