title: ANKRD53 interacts with DDA3 and regulates chromosome integrity during mitosis.
abstract: Spindle dynamics drives chromosome movement and mitotic progression during mitosis. Microtubule (MT)-associated proteins (MAPs) regulate MT stabilization/destabilization and MT polymerization/depolymerization for congression of sister chromatids at the mitotic equator and subsequent segregation toward the spindle poles. Here, we identified ANKRD53 as a novel DDA3-interacting protein through proteomic analysis. Based on expression profiles, ANKRD53 is phosphorylated by mitotic kinases during mitosis. In ANKRD53-depleted HeLa cells, the progression of mitosis was delayed and the number of unaligned chromosomes increased substantially. In addition, spindle MT polymerization decreased and the spindle assembly checkpoint (SAC) was concomitantly activated by the decreased spindle dynamics in ANKRD53-depleted cells. Although ANKRD53 is recruited to the mitotic spindle by DDA3, it counteracts the activity of DDA3 for spindle MT polymerization. Furthermore, ANKRD53 depletion increased the number of bi-nuclei and polylobed nuclei. Thus, ANKRD53 is recruited to the mitotic spindle by DDA3 and acts as a regulator of spindle dynamics and cytokinesis.
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors.forename: Seul
authors.initials: S
authors.lastname: Kim
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea. Electronic address: cyjang@sookmyung.ac.kr.
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Jang
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002875:Chromosomes / Q000502:physiology* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000502:physiology* / Q000648:ultrastructure
chemical_list: C584515:ANKRD55 protein, human; D002352:Carrier Proteins; C485740:PSRC1 protein, human; D010750:Phosphoproteins
keywords: ANKRD53; Chromosome movement; DDA3; Mitotic spindle; Spindle dynamics