title: Wood reinforcement of poplar by rice NAC transcription factor.
abstract: Lignocellulose, composed of cellulose, hemicellulose, and lignin, in the secondary cell wall constitutes wood and is the most abundant form of biomass on Earth. Enhancement of wood accumulation may be an effective strategy to increase biomass as well as wood strength, but currently only limited research has been undertaken. Here, we demonstrated that OsSWN1, the orthologue of the rice NAC Secondary-wall Thickening factor (NST) transcription factor, effectively enhanced secondary cell wall formation in the Arabidopsis inflorescence stem and poplar (Populus tremula×Populus tremuloides) stem when expressed by the Arabidopsis NST3 promoter. Interestingly, in transgenic Arabidopsis and poplar, ectopic secondary cell wall deposition in the pith area was observed in addition to densification of the secondary cell wall in fiber cells. The cell wall content or density of the stem increased on average by up to 38% and 39% in Arabidopsis and poplar, respectively, without causing growth inhibition. As a result, physical strength of the stem increased by up to 57% in poplar. Collectively, these data suggest that the reinforcement of wood by NST3pro:OsSWN1 is a promising strategy to enhance wood-biomass production in dicotyledonous plant species.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Sakamoto
authors.affiliation: Forestry and Forest Products Research Institute, Forest Bio-Research Center, Hitachi, Ibaraki 319-1301, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Takata
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Oshima
authors.affiliation: Technology Center, Taisei Corporation, Nase-cho 344-1, Totsuka-ku, Yokohama, Kanagawa 245-0051, Japan.
authors.forename: Kouki
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Forestry and Forest Products Research Institute, Forest Bio-Research Center, Hitachi, Ibaraki 319-1301, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Taniguchi
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D055595:Mechanical Phenomena; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics* / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014934:Wood
chemical_list: D014157:Transcription Factors
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