title: Proposal of a new method for subtyping of Mycobacterium kansasii based upon PCR restriction enzyme analysis of the tuf gene.
abstract: Within this study, a new, rapid method for subtyping of Mycobacterium kansasii was developed based on the sequence analysis of the tuf gene coding for the Tu (thermo-unstable) elongation factor (EF-Tu). The method involves PCR amplification of ca. 740-bp tuf gene fragment, followed by digestion with the MvaI restriction endonuclease.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096, Warsaw, Poland. Electronic address: zofiabakula@biol.uw.edu.pl.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Bakuła
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096, Warsaw, Poland. Electronic address: magda.modrzejewska@wp.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Modrzejewska
authors.affiliation: Department of Internal Medicine, Pulmonology, and Allergology, Warsaw Medical University, Żwirki i Wigury 61, 02-091, Warsaw, Poland. Electronic address: aleksandra.safianowska@wum.edu.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Safianowska
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, PO Box 9101, 6500HB, Nijmegen, The Netherlands. Electronic address: vaningen.jakko@gmail.com.
authors.forename: Jakko
authors.initials: J
authors.lastname: van Ingen
authors.affiliation: Department of Internal Medicine, Pulmonology, and Allergology, Warsaw Medical University, Żwirki i Wigury 61, 02-091, Warsaw, Poland. Electronic address: m.proboszcz@wp.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Proboszcz
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096, Warsaw, Poland. Electronic address: jbielecki@biol.uw.edu.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bielecki
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096, Warsaw, Poland. Electronic address: t.jagielski@biol.uw.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Jagielski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods*; D019909:Mycobacterium kansasii / Q000145:classification* / Q000235:genetics*; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D055815:Young Adult
chemical_list: D010444:Peptide Elongation Factor Tu
keywords: Mycobacterium kansasii; PCR restriction enzyme analysis; Subtyping; tuf