title: Avian leukosis virus subgroup J triggers caspase-1-mediated inflammatory response in chick livers.
abstract: Many pathogens trigger caspase-1-mediated innate immune responses. Avian leukosis virus subgroup J (ALV-J) causes serious immunosuppression and diverse tumors in chicks. The caspase-1 inflammasome mechanism of response to ALV-J invading remains unclear. Here we investigated the expression of caspase-1, the inflammasome adaptor NLRP3, IL-1β and IL-18 in response to ALV-J infection in the liver of chick. We found caspase-1 mRNA expression was elevated at 5 dpi and peaked at 7 dpi in ALV-J infected animals. Corresponding to this, the expressions of NLRP3 and proinflammatory cytokines IL-1β and IL-18 were significantly increased at 5 or 7 dpi. In addition, caspase-1 protein expression and inflammatory cell infiltration were induced after virus infection. These results indicated that ALV-J infection could trigger the caspase-1- mediated inflammatory response in chicks. Thus, an understanding of the inflammatory responses can provide a better insight into the pathogenicity of ALV-J and a possible anti-virus target for ALV-J infection.
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China. Electronic address: liuxuelan203@163.com.
authors.forename: Xue-lan
authors.initials: XL
authors.lastname: Liu
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Shan
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Jia
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Jin-jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Ya-rong
authors.initials: YR
authors.lastname: Wu
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Fa-zhi
authors.initials: FZ
authors.lastname: Xu
authors.affiliation: College of Animal Science & Technology, Anhui Agricultural University, 130Changjiang Ave, Hefei 230036, Anhui China.
authors.forename: Jin-nian
authors.initials: JN
authors.lastname: Li
mesh_terms: D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics / Q000472:pathogenicity*; D020170:Caspase 1 / Q000032:analysis*; D020869:Gene Expression Profiling; D005838:Genotype; D007249:Inflammation / Q000473:pathology*; D020382:Interleukin-18 / Q000032:analysis; D053583:Interleukin-1beta / Q000032:analysis; D008099:Liver / Q000473:pathology*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013997:Time Factors
chemical_list: D020382:Interleukin-18; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D012333:RNA, Messenger; D020170:Caspase 1
keywords: ALV-J; Caspase-1; Inflammatory response; Trigger