title: Myosin IXa Binds AMPAR and Regulates Synaptic Structure, LTP, and Cognitive Function.
abstract: Myosin IXa (Myo9a) is a motor protein that is highly expressed in the brain. However, the role of Myo9a in neurons remains unknown. Here, we investigated Myo9a function in hippocampal synapses. In rat hippocampal neurons, Myo9a localizes to the postsynaptic density (PSD) and binds the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) GluA2 subunit. Myo9a(+/-) mice displayed a thicker PSD and increased levels of PSD95 and surface AMPAR expression. Furthermore, synaptic transmission, long-term potentiation (LTP) and cognitive functions were impaired in Myo9a(+/-) mice. Together, these results support a key role for Myo9a in controlling the molecular structure and function of hippocampal synapses.
authors.affiliation: CNR Institute of NeuroscienceMilano, Italy; Institute of Biophysics, Medical University of GrazGraz, Austria.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Folci
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Murru
authors.affiliation: Department of Medical Biotechnologies and Translational Medicine (BIOMETRA), Università degli Studi di MilanoMilano, Italy; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di MilanoMilano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Vezzoli
authors.affiliation: Department of Medical Biotechnologies and Translational Medicine (BIOMETRA), Università degli Studi di Milano Milano, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ponzoni
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Gerosa
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Moretto
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Longo
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Zapata
authors.affiliation: Department of Medical Biotechnologies and Translational Medicine (BIOMETRA), Università degli Studi di Milano Milano, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Braida
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pistillo
authors.affiliation: Institute of Molecular Cell Biology, Westfälische Wilhelms-Universität Münster Münster, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Bähler
authors.affiliation: CNR Institute of NeuroscienceMilano, Italy; Department of Medical Biotechnologies and Translational Medicine (BIOMETRA), Università degli Studi di MilanoMilano, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Francolini
authors.affiliation: CNR Institute of NeuroscienceMilano, Italy; Department of Medical Biotechnologies and Translational Medicine (BIOMETRA), Università degli Studi di MilanoMilano, Italy.
authors.forename: Mariaelvina
authors.initials: M
authors.lastname: Sala
authors.affiliation: CNR Institute of Neuroscience Milano, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bassani
keywords: AMPAR; LTP; Myosin IXa; PSD; hippocampal synapses
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