title: Maxillary sinus floor augmentation and dental implant placement using dentin matrix protein-1 gene-modified bone marrow stromal cells mixed with deproteinized boving bone: A comparative study in beagles.
abstract: OBJECTIVE
  The aim of the study was to evaluate the effects of the combined use of dentin matrix protein-1 (DMP1) gene-modified bone marrow stromal cells (BMSCs) and Bio-Oss(®) for maxillary sinus floor augmentation (MSFA) implant placement in dogs.
  
  
  METHODS
  BMSCs were derived from bone marrow of six beagles and cultured. The cells were transduced with a lentiviral vector overexpressing the DMP1 gene and enhanced green fluorescent protein (EGFP) gene (Lenti-DMP1/EGFP) in test group, and with a lentiviral vector encoding EGFP gene (Lenti-EGFP) in control group. Six dogs received sinus augmentations using the bilateral approach with a simultaneous implant placement at each site respectively. At the same concentration, 2×10(7) cells/ml, one sinus was grafted using a mixture of autologous DMP1/EGFP gene-modified BMSCs and Bio-Oss(®) (DMP1 group), and the contralateral sinus was grafted with autologous EGFP gene-modified bMSCs and Bio-Oss(®) (EGFP group). After a 3 month healing period, bone regeneration and osseointegration were evaluated using histologic and histomorphometric methods.
  
  
  RESULTS
  The bone-implant contact (BIC) and the bone area fraction in the DMP1 group (BIC: 34.67%±8.23%, bone area fraction: 35.16%±3.32%) were significantly greater compared with the EGFP group (BIC: 26.06%±5.16%, bone area fraction: 20.74%±1.63%) (P<0.05). No significant difference between the residual bone substitute material volume (BSMV) in the DMP1 group (35.86±7.35) and the EGFP group (32.16±9.16) was found in our study (P>0.05).
  
  
  CONCLUSIONS
  BMSCs modified with the DMP1 gene can be used as an adjunct to Bio-Oss(®) to enhance new bone formation and the osseointegration of dental implants in MSFA of dogs.
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, Department of Implantation, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China. Electronic address: xindyliu@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, Department of Implantation, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China. Electronic address: qiong1988@gmail.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Li
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, Department of Implantation, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China. Electronic address: w_fang168@hotmail.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, Department of Implantation, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China. Electronic address: zuolin@mail.tongji.edu.cn.
authors.forename: Zuolin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000627:therapeutic use; D016025:Bone Transplantation / Q000379:methods*; D003758:Dental Implantation, Endosseous / Q000379:methods*; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000627:therapeutic use; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008437:Maxilla / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D008903:Minerals; D016348:Osseointegration; D010012:Osteogenesis; D059546:Sinus Floor Augmentation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D016326:Extracellular Matrix Proteins; D008903:Minerals; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Bone marrow stromal cells; Dental implant; Dentin matrix protein 1; Gene therapy; Maxillary sinus floor augmentation