title: Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals.
abstract: Understanding the role of protein turnover in the maintenance of proteostasis requires accurate measurements of the rates of replacement of proteins in complex systems, such as intact animals. Moreover, any investigation of allometric scaling of protein turnover is likely to include species for which fully annotated proteomes are not available. We have used dietary administration of stable isotope labeled lysine to assess protein turnover rates for proteins from four tissues in the bank vole,Myodes glareolus The annotated genome for this species is not available, so protein identification was attained through cross-species matching to the mouse. For proteins for which confident identifications were derived, the pattern of lysine incorporation over 40 days was used to define the rate of synthesis of individual proteins in the four tissues. The data were heavily filtered to retain a very high quality dataset of turnover rates for 1088 proteins. Comparative analysis of the four tissues revealed different median rates of degradation (kidney: 0.099 days(-1); liver 0.136 days(-1); heart, 0.054 days(-1), and skeletal muscle, 0.035 days(-1)). These data were compared with protein degradation rates from other studies on intact animals or from cells in culture and indicate that both cell type and analytical methodology may contribute to variance in turnover data between different studies. These differences were not only due to tissue-specific proteins but were reflected in gene products common to all tissues. All data are available via ProteomeXchange with identifier PXD002054.
authors.affiliation: From the ‡Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L697ZB, UK;
authors.forename: Dean E
authors.initials: DE
authors.lastname: Hammond
authors.affiliation: From the ‡Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L697ZB, UK;
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Claydon
authors.affiliation: From the ‡Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L697ZB, UK;
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Simpson
authors.affiliation: §Mammalian Behaviour and Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, CH64 7TE, UK.
authors.forename: Dominic
authors.initials: D
authors.lastname: Edward
authors.affiliation: §Mammalian Behaviour and Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, CH64 7TE, UK.
authors.forename: Paula
authors.initials: P
authors.lastname: Stockley
authors.affiliation: §Mammalian Behaviour and Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, CH64 7TE, UK.
authors.forename: Jane L
authors.initials: JL
authors.lastname: Hurst
authors.affiliation: From the ‡Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L697ZB, UK; r.beynon@liverpool.ac.uk.
authors.forename: Robert J
authors.identifier: http://orcid.org/0000-0003-0857-495X
authors.initials: RJ
authors.lastname: Beynon
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000378:metabolism*; D007553:Isotope Labeling; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008239:Lysine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity; D059748:Proteolysis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D014018:Tissue Distribution
chemical_list: D020543:Proteome; D008239:Lysine
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