title: Influence of Solution Chemistry and Soft Protein Coronas on the Interactions of Silver Nanoparticles with Model Biological Membranes.
abstract: The influence of solution chemistry and soft protein coronas on the interactions between citrate-coated silver nanoparticles (AgNPs) and model biological membranes was investigated by assembling supported lipid bilayers (SLBs) composed of zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) on silica crystal sensors in a quartz crystal microbalance with dissipation monitoring (QCM-D). Our results show that the deposition rates of AgNPs on unmodified silica surfaces increased with increasing electrolyte concentrations under neutral pH conditions. Similar trends were observed when AgNPs were deposited on SLBs, hence indicating that the deposition of AgNPs on model cell membranes was controlled by electrostatic interactions. In the presence of human serum albumin (HSA) proteins at both pH 7 and pH 2, the colloidal stability of AgNPs was considerably enhanced due to the formation of HSA soft coronas surrounding the nanoparticles. At pH 7, the deposition of AgNPs on SLBs was suppressed in the presence of HSA due to steric repulsion between HSA-modified AgNPs and SLBs. In contrast, pronounced deposition of HSA-modified AgNPs on SLBs was observed at pH 2. This observation was attributed to the reduction of electrostatic repulsion as well as conformation changes of adsorbed HSA under low pH conditions, resulting in the decrease of steric repulsion between AgNPs and SLBs.
authors.affiliation: School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University , 1239 Siping Road, Shanghai 200092, PR China.|Department of Geography and Environmental Engineering, Johns Hopkins University , Baltimore, Maryland 21218-2686, United States.
authors.forename: Qiaoying
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Geography and Environmental Engineering, Johns Hopkins University , Baltimore, Maryland 21218-2686, United States.
authors.forename: Myunghee
authors.initials: M
authors.lastname: Lim
authors.affiliation: Department of Geography and Environmental Engineering, Johns Hopkins University , Baltimore, Maryland 21218-2686, United States.
authors.forename: Xitong
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University , 1239 Siping Road, Shanghai 200092, PR China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Geography and Environmental Engineering, Johns Hopkins University , Baltimore, Maryland 21218-2686, United States.
authors.forename: Kai Loon
authors.initials: KL
authors.lastname: Chen
mesh_terms: D019343:Citric Acid / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000737:chemistry; D000066970:Protein Corona / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D012709:Serum Albumin / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D012996:Solutions; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D000066970:Protein Corona; D012709:Serum Albumin; D012996:Solutions; D019343:Citric Acid; D012834:Silver; D012822:Silicon Dioxide; C017251:1,2-oleoylphosphatidylcholine