title: Expression of uPAR in human trophoblast and its role in trophoblast invasion.
abstract: Placental trophoblast cells differentiate into invasive trophoblasts or syncytiotrophoblasts. Abnormal trophoblast invasion results in pregnancy-associated disease and abortion. uPAR is a cell membrane-bound glycosylated protein, involved in physiological and pathological processes. However, uPAR expression in villi during threatened abortion and its role in trophoblast differentiation are unclear. We determined that, uPAR expression in the villi was reduced in threatened abortion patients than that in normal pregnancy. uPARsiRNA inhibited the potential for trophoblast migration and invasion in explants culture and HTR8/SVneo cells. It also enhanced forskolin-induced fusion of HTR8/SVneo cells. Overall, this study provides a possible reason for abortion.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian 116044, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and GlycoengineeringDalian 116044, People's Republic of China; College of Laboratory Medicine, Dalian Medical UniversityDalian, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian 116044, People's Republic of China.
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Cui
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian 116044, People's Republic of China.
authors.forename: Kui-Xing
authors.initials: KX
authors.lastname: Dai
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian 116044, People's Republic of China.
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Yang
authors.affiliation: College of Laboratory Medicine, Dalian Medical University Dalian, People's Republic of China.
authors.forename: Fa-Sheng
authors.initials: FS
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian 116044, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yan
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D016022:Case-Control Studies; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D002465:Cell Movement; D005576:Colforsin / Q000494:pharmacology; D010064:Embryo Implantation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D011247:Pregnancy; D034622:RNA Interference; D055293:Receptors, Urokinase Plasminogen Activator / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D046509:Tissue Culture Techniques; D014162:Transfection; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: C524898:PLAUR protein, human; D055293:Receptors, Urokinase Plasminogen Activator; D005576:Colforsin
keywords: abortion; migration/invasion; syncytialization; trophoblast; uPAR
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