title: Knockdown of USP39 by lentivirus-mediated RNA interference suppresses the growth of oral squamous cell carcinoma.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) is a frequently diagnosed life-threatening oral cancer worldwide and has become one of the leading causes of cancer-related mortality. However, the pathogenesis of this disease is very limited.
  
  
  OBJECTIVE
  In this study, we aimed to investigate the functional relationship between OSCC and a potential tumor related gene ubiquitin-specific proteases 39 (USP39).
  
  
  METHODS
  The lentivirus-based RNA interference was utilized to knock down USP39 expression in human OSCC CAL27 cells. The effect of USP39 on cell proliferation was detected by MTT and colony formation assays.
  
  
  RESULTS
  The results uncovered that the proliferation rate was significantly decreased in specific USP39-targeting lentivirus infected cells compared to control lentivirus infected cells. The colony formation capacity was also attenuated in CAL27 cells after USP39 knockdown. Moreover, knockdown of USP39 arrested CAL27 cells in S and G1/M phases of the cell cycle. Furthermore, USP39 silencing induced apoptosis of CAL27 cells via activations of Caspase 3 and PARP.
  
  
  CONCLUSIONS
  In conclusion, the inhibition of USP39 in CAL27 cells suppressed cell growth probably via induction cell cycle arrest and apoptosis. USP39 might act as an oncogenic factor in OSCC and could be a potential molecular target for OSCC gene therapy.
authors.affiliation: Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong, China.|Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.|Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Ke-Yi
authors.initials: KY
authors.lastname: Li
authors.affiliation: Department of Pathology, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.|Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Jiang
authors.affiliation: Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong, China.|Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.|Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.|Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Chun-Peng
authors.initials: CP
authors.lastname: Xia
authors.affiliation: Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.|Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Chen
authors.affiliation: Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.|Key Lab of Oral and Maxillofacial Medicine, Liaocheng People's Hospital, Liaocheng, Shandong, China.
authors.forename: Qiao-Zhi
authors.initials: QZ
authors.lastname: Yang
authors.affiliation: Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Beijing Pediatric Hospital, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D064570:Ubiquitin-Specific Proteases / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C579218:USP39 protein, human; D064570:Ubiquitin-Specific Proteases
keywords: Drug target; RNA interference; oral squamous cell carcinoma; ubiquitin-specific proteases 39