title: Enzymatic hydrolysis of biomass from wood.
abstract: Current research and development in cellulosic ethanol production has been focused mainly on agricultural residues and dedicated energy crops such as corn stover and switchgrass; however, woody biomass remains a very important feedstock for ethanol production. The precise composition of hemicellulose in the wood is strongly dependent on the plant species, therefore different types of enzymes are needed based on hemicellulose complexity and type of pretreatment. In general, hardwood species have much lower recalcitrance to enzymes than softwood. For hardwood, xylanases, beta-xylosidases and xyloglucanases are the main hemicellulases involved in degradation of the hemicellulose backbone, while for softwood the effect of mannanases and beta-mannosidases is more relevant. Furthermore, there are different key accessory enzymes involved in removing the hemicellulosic fraction and increasing accessibility of cellulases to the cellulose fibres improving the hydrolysis process. A diversity of enzymatic cocktails has been tested using from low to high densities of biomass (2-20% total solids) and a broad range of results has been obtained. The performance of recently developed commercial cocktails on hardwoods and softwoods will enable a further step for the commercialization of fuel ethanol from wood.
authors.affiliation: Department of Biotechnology, Abengoa Research, Campus Palmas Altas, C/Energía Solar no. 1, Seville, 41014, Spain.
authors.forename: Consolación
authors.initials: C
authors.lastname: Álvarez
authors.affiliation: Department of Biotechnology, Abengoa Research, Campus Palmas Altas, C/Energía Solar no. 1, Seville, 41014, Spain.
authors.forename: Francisco Manuel
authors.initials: FM
authors.lastname: Reyes-Sosa
authors.affiliation: Department of Biotechnology, Abengoa Research, Campus Palmas Altas, C/Energía Solar no. 1, Seville, 41014, Spain.
authors.forename: Bruno
authors.initials: B
authors.lastname: Díez
mesh_terms: D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D009097:Multienzyme Complexes / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D014934:Wood / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D011134:Polysaccharides; C007916:hemicellulose; D006026:Glycoside Hydrolases; C023305:hemicellulase
references.citation: Bioresour Technol. 2010 May;101(9):3106-14
references.pmid: 20061141
references.citation: Carbohydr Res. 2000 Dec 1;329(4):807-15
references.pmid: 11125823
references.citation: Fungal Genet Biol. 2014 Nov;72:82-90
references.pmid: 24853079
references.citation: J Biol Chem. 1993 Jun 15;268(17):12274-81
references.pmid: 8509364
references.citation: Biotechnol Bioeng. 2008 Sep 1;101(1):39-48
references.pmid: 18421796
references.citation: Carbohydr Res. 2003 Mar 14;338(6):525-34
references.pmid: 12668108
references.citation: Nature. 2014 Mar 13;507(7491):152-3
references.pmid: 24622185
references.citation: Fungal Genet Biol. 2014 Nov;72:10-20
references.pmid: 24881579
references.citation: Biotechnol Prog. 2009 Jul-Aug;25(4):1086-93
references.pmid: 19551888
references.citation: Annu Rev Microbiol. 2013;67:477-98
references.pmid: 23808333
references.citation: Front Plant Sci. 2013 May 21;4:118
references.pmid: 23734153
references.citation: Science. 1983 Aug 12;221(4611):661-3
references.pmid: 17787736
references.citation: Trends Biotechnol. 2001 May;19(5):172-7
references.pmid: 11301129
references.citation: Bioresour Technol. 2002 May;83(1):1-11
references.pmid: 12058826
references.citation: Biotechnol Biofuels. 2011 Dec 20;4(1):60
references.pmid: 22185437
references.citation: Appl Environ Microbiol. 1999 Mar;65(3):916-22
references.pmid: 10049842
references.citation: FEBS Lett. 2006 Aug 21;580(19):4597-601
references.pmid: 16876163
references.citation: Bioresour Technol. 2009 Dec;100(23):5890-7
references.pmid: 19643602
references.citation: Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5
references.pmid: 24270786
references.citation: Bioresour Technol. 2008 Jan;99(1):137-45
references.pmid: 17223555
references.citation: Mycologia. 2013 Nov-Dec;105(6):1412-27
references.pmid: 23935027
references.citation: Bioresour Technol. 2010 Jul;101(13):4992-5002
references.pmid: 19969450
references.citation: Nat Biotechnol. 2008 Feb;26(2):169-72
references.pmid: 18259168
references.citation: Bioresour Technol. 2009 Apr;100(8):2411-8
references.pmid: 19119005
references.citation: Biotechnol Biofuels. 2013 Mar 21;6(1):41
references.pmid: 23514094
references.citation: Met Ions Biol Syst. 2000;37:559-86
references.pmid: 10693145
references.citation: Appl Environ Microbiol. 1994 Dec;60(12):4509-16
references.pmid: 7811086
references.citation: J Agric Food Chem. 2010 Jun 23;58(12):7233-8
references.pmid: 20509690
references.citation: Appl Environ Microbiol. 2012 Mar;78(5):1596-600
references.pmid: 22210217
references.citation: FEBS Lett. 1996 Aug 5;391(1-2):144-8
references.pmid: 8706903
references.citation: Appl Biochem Biotechnol. 2005 Spring;121-124:1069-79
references.pmid: 15930582
references.citation: Bioresour Technol. 2012 Oct;121:61-7
references.pmid: 22854131
references.citation: Biotechnol Biofuels. 2009 Oct 01;2(1):24
references.pmid: 19796378
references.citation: Microbiol Mol Biol Rev. 2001 Dec;65(4):497-522, table of contents
references.pmid: 11729262
references.citation: Biotechnol Prog. 1999 Oct 1;15(5):804-816
references.pmid: 10514250