title: Characterization of enzymatic profiles of Aedes aegypti strains from the State of Rio Grande do Norte, Brazil.
abstract: This study was conducted in four strains of Aedes aegypti mosquitoes to evaluate the enzymatic activity profiles in the city of Mossoró, Rio Grande do Norte, and correlate them with biochemical mechanisms of resistance to insecticides. Mosquitos were used to quantify the following detoxification enzymes: Mixed-Function Oxidase (MFO), PNPA-esterase (PNPA-EST), and Acetylcholinesterase (AChE). The profiles were compared statistically with profiles from the Rockefeller strain, through the Kruskal-Wallis test and Dunn's multiple comparisons (p < 0.05). The 99 percentile of the values of enzyme activity from the reference strain was calculated for each enzyme, and the percentage of individuals above the 99 percentile was quantified. The enzyme activities were classified as "Unchanged" (< 15%), "Identified change" (> 15% and < 50%), and "Substantially changed" (> 50%). The statistical analysis revealed significant differences in the MFO and AChE profiles, which are fundamental in the determination of profiles of resistance to insecticides. Three populations were classified as "Substantially changed" for MFO. The altered enzymatic activity showed that the changes could have an important role in exposing resistance to insecticides.
authors.affiliation: Faculdade de Ciências da Saúde, Universidade do Estado do Rio Grande do Norte, Mossoró, RN, Brasil, renanunes@uol.com.br.
authors.forename: Renan Flávio de França
authors.initials: RF
authors.lastname: Nunes
authors.affiliation: Faculdade de Ciências da Saúde, Universidade do Estado do Rio Grande do Norte, Mossoró, RN, Brasil, renanunes@uol.com.br.
authors.forename: Marcos Antonio de
authors.initials: MA
authors.lastname: Souza
authors.affiliation: Universidade Federal Rural do Semi-Árido, Brasil.
authors.forename: Jéssica Costa de
authors.initials: JC
authors.lastname: Oliveira
authors.affiliation: Faculdade de Ciências da Saúde, Universidade do Estado do Rio Grande do Norte, Mossoró, RN, Brasil, renanunes@uol.com.br.
authors.forename: Richardeson Fagner de Oliveira
authors.initials: RF
authors.lastname: Grangeiro
authors.affiliation: Faculdade de Ciências da Saúde, Universidade do Estado do Rio Grande do Norte, Mossoró, RN, Brasil, renanunes@uol.com.br.
authors.forename: Maria Jocileide de Medeiros
authors.initials: MJ
authors.lastname: Marinho
authors.affiliation: Faculdade de Ciências da Saúde, Universidade do Estado do Rio Grande do Norte, Mossoró, RN, Brasil, renanunes@uol.com.br.
authors.forename: Wogelsanger Oliveira
authors.initials: WO
authors.lastname: Pereira
mesh_terms: D000330:Aedes / Q000201:enzymology*; D000818:Animals; D001938:Brazil; D007305:Insecticide Resistance; D007306:Insecticides
chemical_list: D007306:Insecticides