title: Design and synthesis of fluorescent and biotin tagged probes for the study of molecular actions of FAF1 inhibitor.
abstract: To study the molecular action of ischemic Fas-mediated cell death inhibitor, we prepared fluorescent-tagged and biotin-tagged probes of the potent inhibitor, KR-33494, of ischemic cell death. We used the molecular modeling technique to find the proper position for attaching those probes with minimum interference in the binding process of probes with Fas-mediated cell death target, FAF1.
authors.affiliation: Graduate School of New Drug Discovery and Development, Chungnam National University, Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Sung-eun
authors.initials: SE
authors.lastname: Yoo
authors.affiliation: Department of Bioscience and Biotechnology, Chungnam National University, Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Changsun
authors.initials: C
authors.lastname: Yu
authors.affiliation: Graduate School of New Drug Discovery and Development, Chungnam National University, Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: SeoHee
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Bioscience and Biotechnology, Chungnam National University, Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Eunhee
authors.initials: E
authors.lastname: Kim
authors.affiliation: Graduate School of New Drug Discovery and Development, Chungnam National University, Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Nam Sook
authors.initials: NS
authors.lastname: Kang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins; D001665:Binding Sites; D001710:Biotin / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015195:Drug Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D062105:Molecular Docking Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C490780:FAF1 protein, human; D005456:Fluorescent Dyes; D001710:Biotin; D006861:Hydrogen Peroxide
keywords: Biotin tagged probe; FAF1; Fas-mediated cell death pathways; Fluorescence tagged probe; KR-33493