title: Effectiveness of rosiglitazone in reducing flexion contracture in a rabbit model of arthrofibrosis with surgical capsular release: A biomechanical, histological, and genetic analysis.
abstract: OBJECTIVE
  Animal models have been developed that allow simulation of post-traumatic joint contracture. One such model involves contracture-forming surgery followed by surgical capsular release. This model allows testing of antifibrotic agents, such as rosiglitazone.
  
  
  METHODS
  A total of 20 rabbits underwent contracture-forming surgery. Eight weeks later, the animals underwent a surgical capsular release. Ten animals received rosiglitazone (intramuscular initially, then orally). The animals were sacrificed following 16 weeks of free cage mobilisation. The joints were tested biomechanically, and the posterior capsule was assessed histologically and via genetic microarray analysis.
  
  
  RESULTS
  There was no significant difference in post-traumatic contracture between the rosiglitazone and control groups (33° (standard deviation (sd) 11) vs 37° (sd14), respectively; p = 0.4). There was no difference in number or percentage of myofibroblasts. Importantly, there were ten genes and 17 pathways that were significantly modulated by rosiglitazone in the posterior capsule.
  
  
  CONCLUSIONS
  Rosiglitazone significantly altered the genetic expression of the posterior capsular tissue in a rabbit model, with ten genes and 17 pathways demonstrating significant modulation. However, there was no significant effect on biomechanical or histological properties.Cite this article: M. P. Abdel. Effectiveness of rosiglitazone in reducing flexion contracture in a rabbit model of arthrofibrosis with surgical capsular release: A biomechanical, histological, and genetic analysis. Bone Joint Res 2016;5:11-17. doi: 10.1302/2046-3758.51.2000593.
authors.affiliation: Ohio State Orthopedic Surgery, 915 Olentangy River Rd, Suite 3200 Columbus, OH 43212, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Barlow
authors.affiliation: Mayo Clinic, 200 First Street SW, Rochester, MN 55901, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Morrey
authors.affiliation: San Antonio Orthopaedic Group and Burkhart Research Institute for Orthopaedics, 150 E. Sonterra Blvd. Suite 300 San Antonio, Texas 78258, USA.
authors.forename: R U
authors.initials: RU
authors.lastname: Hartzler
authors.affiliation: Stanford University School of Medicine, 450 Broadway St. MC: 6342, Redwood City, CA 94063, USA.
authors.forename: D
authors.initials: D
authors.lastname: Arsoy
authors.affiliation: Mayo Clinic, 200 First Street SW, Rochester, MN 55901, USA.
authors.forename: S
authors.initials: S
authors.lastname: Riester
authors.affiliation: Mayo Clinic, 200 First Street SW, Rochester, MN 55901, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: van Wijnen
authors.affiliation: Mayo Clinic, 200 First Street SW, Rochester, MN 55901, USA.
authors.forename: B F
authors.initials: BF
authors.lastname: Morrey
authors.affiliation: Mayo Clinic, 200 First Street SW, Rochester, MN 55901, USA.
authors.forename: J
authors.initials: J
authors.lastname: Sanchez-Sotelo
authors.affiliation: Mayo Clinic, 200 First Street SW, Rochester, MN 55901, USA abdel.matthew@mayo.edu.
authors.forename: M P
authors.initials: MP
authors.lastname: Abdel
keywords: arthrofibrosis; joint contractures; knee; rosiglitazone; surgical release
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