title: [The effect of signal transduction pathway of triggering receptor-1 expressed on myeloid cells in acute lung injury induced by paraquat in rats].
abstract: OBJECTIVE
  To investigate the transduction pathway of triggering receptor-1 expressed on myeloid cells (TREM-1) in acute lung injure induced by paraquat in rats through the activating or blocking TREM-1, to observe the effect of signal transduction pathway in the acute lung injure induced-paraquat.
  
  
  METHODS
  80 SD rats were randomly divided into normal saline control group (n=20) , PQ poisoning group (n=20) , antibody group (n=20) , and LP17 group (n=20). poisoning group, antibody group and LP17 group were given saline diluting PQ 80 mg/kg of disposable lavage after 2 h, a single set of intraperitoneal injection of anti-TREM-1 mAb (250 g/kg) , tail intravenous LP17 group synthetic peptide (3.5 mg/kg) , poisoning group was given equal normal saline intraperitoneal injection, control group given normal saline 1 mg/kg after 2 h after lavage, given the amount of intraperitoneal injection of saline solution. The expression of NF-κB in lung tissue was determined by immunohistochemistry.The levels of TNF-a, IL-10, TREM-1, and soluble TREM-1 (sTREM-1) in lung tissue and serum were measured by ELISA. Pathology changes of lung were observed under light microscope, and lung score of pathology was compared.
  
  
  RESULTS
  Administration of anti-TREM-1 mAb after PQ poisoning modeling significantly increased the NF-κB expression in lung tissue at 48 h, resulting in a large number of pro-inflammatory cytokines releasing in the lung tissue and serum and lung pathology injury score increasing.Administration of LP17 after modeling significantly down-·regulated the expressions of NF-κB and proinflammatory cytokines, while led to a slight increase of anti-inflammatory cytokines and a decline of lung pathology injury score.
  
  
  CONCLUSIONS
  TREM-1 may involve in inflammatory response by promoting the generation of inflammatory factors via NF-κB pathway, thus lead to lung pathological changes.
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; E-mail: Tuling@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Tu
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Diming
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Department of Clinical Laboratory, The fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D016753:Interleukin-10 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010269:Paraquat / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000378:metabolism*; D015398:Signal Transduction; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D011971:Receptors, Immunologic; C547511:TREM1 protein, rat; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D010269:Paraquat