title: An optimized method for neurotransmitters and their metabolites analysis in mouse hypothalamus by high performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry.
abstract: Neurotransmitters (NTs) and their metabolites are known to play an essential role in maintaining various physiological functions in nervous system. However, there are many difficulties in the detection of NTs together with their metabolites in biological samples. A new method for NTs and their metabolites detection by high performance liquid chromatography coupled with Q Exactive hybrid quadruple-orbitrap high-resolution accurate mass spectrometry (HPLC-HRMS) was established in this paper. This method was a great development of the applying of Q Exactive MS in the quantitative analysis. This method enabled a rapid quantification of ten compounds within 18min. Good linearity was obtained with a correlation coefficient above 0.99. The concentration range of the limit of detection (LOD) and the limit of quantitation (LOQ) level were 0.0008-0.05nmol/mL and 0.002-25.0nmol/mL respectively. Precisions (relative standard deviation, RSD) of this method were at 0.36-12.70%. Recovery ranges were between 81.83% and 118.04%. Concentrations of these compounds in mouse hypothalamus were detected by Q Exactive LC-MS technology with this method.
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
authors.forename: Zong-Lin
authors.initials: ZL
authors.lastname: Yang
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China; Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: syliu@ciac.jl.cn.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007031:Hypothalamus / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051379:Mice; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D018377:Neurotransmitter Agents
keywords: Hypothalamus; Mouse; Neurotransmitters (NTs); Q Exactive MS; Quantitative detection