title: Nitrate repletion strategy for enhancing lipid production from marine microalga Tetraselmis sp.
abstract: The cell growth rate and cellular lipid content of microalgae are affected by the nitrogen levels during cultivation. The growth rate and lipid content of marine microalga Tetraselmis sp. was found to increase under nitrate replete conditions, but not under deplete conditions. Thus, in order to enhance the lipid productivity of Tetraselmis sp., a two-stage culture process utilizing nitrate replete condition was applied. When the cells were cultivated in F/2 medium for five days in the first stage, the obtained lipid content and productivity were 22.4% and 26.7mgL(-1)d(-1), respectively. After second stage of cultivation for a further 36h under nitrate replete conditions with 8.82mM NaNO3, increased biomass concentration of 1.32gL(-1) and lipid content of 30.5% were obtained, with an enhanced lipid productivity of 47.3mgL(-1)d(-1).
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, Yongin 449-728, Republic of Korea.
authors.forename: Garam
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, Yongin 449-728, Republic of Korea.
authors.forename: Jinsung
authors.initials: J
authors.lastname: Bae
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, Yongin 449-728, Republic of Korea. Electronic address: kisay@mju.ac.kr.
authors.forename: Kisay
authors.initials: K
authors.lastname: Lee
mesh_terms: D059001:Aquatic Organisms / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000460:Chlorophyta / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D009566:Nitrates; D009584:Nitrogen
keywords: FAME profiles; Lipid productivity; Nitrate repletion; Tetraselmis; Two-stage process