title: TatC-dependent translocation of pyoverdine is responsible for the microbial growth suppression.
abstract: Infections are often not caused by a colonization of Pseudomonas aeruginosa alone but by a consortium of other bacteria. Little is known about the impact of P. aeruginosa on the growth of other bacteria upon coinfection. Here, cell-ree culture supernatants obtained from P. aeruginosa suppressed the growth of a number of bacterial strains such as Corynebacterium glutamicum, Bacillus subtilis, Staphylococcus aureus, and Agrobacterium tumefaciens, but had little effect on the growth of Escherichia coli and Salmonella Typhimurium. The growth suppression effect was obvious when P. aeruginosa was cultivated in M9 minimal media, and the suppression was not due to pyocyanin, a well-known antimicrobial toxin secreted by P. aeruginosa. By performing transposon mutagenesis, PA5070 encoding TatC was identified, and the culture supernatant of its mutant did not suppress the growth. HPLC analysis of supernatants showed that pyoverdine was a secondary metabolite present in culture supernatants of the wild-type strain, but not in those of the PA5070 mutant. Supplementation of FeCl2 as a source of iron compromised the growth suppression effect of supernatants and also recovered biofilm formation of S. aureus, indicating that pyoverdine-mediated iron acquisition is responsible for the growth suppression. Thus, this study provides the action of TatC-dependent pyoverdine translocation for the growth suppression of other bacteria, and it might aid understanding of the impact of P. aeruginosa in the complex community of bacterial species upon coinfection.
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700, Republic of Korea.
authors.forename: Yeji
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700, Republic of Korea.
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea.|New Drug Discovery Lab, Hyundai Pharmaceutical Co., Ltd., Yongin, 449-060, Republic of Korea.
authors.forename: Hyung Eun
authors.initials: HE
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea.
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA.
authors.forename: Shouguang
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong, 339-700, Republic of Korea. haunhwan@korea.ac.kr.
authors.forename: Un-Hwan
authors.initials: UH
authors.lastname: Ha
mesh_terms: D000898:Antibiosis; D004251:DNA Transposable Elements; D007501:Iron / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009842:Oligopeptides / Q000378:metabolism*; D021381:Protein Transport; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D004251:DNA Transposable Elements; D026901:Membrane Transport Proteins; D009842:Oligopeptides; C042453:pyoverdin; D007501:Iron
keywords: TatC; growth suppression; pyoverdine
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