title: Characterization and determination of isomers in plants using trace matrix solid phase dispersion via ultrahigh performance liquid chromatography coupled with an ultraviolet detector and quadrupole time-of-flight tandem mass spectrometry.
abstract: Here, a novel β-cyclodextrin (β-CD) based trace matrix solid-phase dispersion (MSPD) method was developed to extract isomers from different honeysuckle samples using ultrahigh performance liquid chromatography. The structures of the analytes were characterized and determined via quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance. Under optimum conditions, 25mg of sample was dispersed with 75mg of β-CD, and 0.5mL of methanol was used as the elution solvent. The proposed method produced a good linearity (r(2)>0.99) for the isomers, with limits of detection ranging from 1.62 to 3.33ng/mL. The average recovery values that were obtained from analyzing the spiked samples were calculated to be in the range of 87.04-105.20%. Moreover, the trace MSPD method required less reagents and a shorter extraction time in comparison with the traditional pharmacopoeia method and other reported approaches. The developed method was successfully applied for the determination of three real honeysuckle samples that were collected from different cultivated areas.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Peng
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Qiong-Yao
authors.initials: QY
authors.lastname: Zhu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007536:Isomerism; D031239:Lonicera / Q000737:chemistry*; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D012997:Solvents; D053719:Tandem Mass Spectrometry / Q000379:methods; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012997:Solvents; D047392:beta-Cyclodextrins; D000432:Methanol
keywords: Honeysuckle; Isomer; Quadrupole time-of-flight tandem mass spectrometry; Trace matrix solid phase dispersion; Ultrahigh performance liquid chromatography; β-Cyclodextrin