title: Prevalence of bla(PenA) and bla(OXA) in Burkholderia pseudomallei Isolated from Patients at Sappasitthiprasong Hospital and Their Susceptibility to Ceftazidime and Carbapenems.
abstract: BACKGROUND
  Burkholderia pseudomallei is a causative agent of melioidosis. Ceftazidime is the preferred drug of choice for treatment. However, the motility rate is high in endemic areas.
  
  
  OBJECTIVE
  This study aimed to determine the susceptibility tofour different antimicrobial agents and to detect the β-lactamase genes in B. pseudomallei isolates from patients admitted to Sappasitthiprasong Hospital.
  
  
  METHODS
  85 B. pseudomallei isolates from patients admitted to Sappasitthiprasong Hospital between November 2010 and May 2011 were determined for antimicrobial susceptibility by standard disk diffusion and minimum inhibitory concentration (MIC). Real-time polymerase chain reaction (PCR) was used for the detection of bla(penA) and bla(OXA) in β-lactamase genes.
  
  
  RESULTS
  Almost all of the clinical isolates ofB. pseudomallei were susceptible to ceftazidime and imipenem. Cefatzidime MIC was ≤ 1-16 μg/ml and imipenem MIC was ≤ 1-4 μg/ml. The real-time PCR revealed that more than 90% of B. pseudomallei isolates carried bla(PenA) and bla(OXA).
  
  
  CONCLUSIONS
  Although the clinical isolates of B. pseudomallei were susceptible to ceftazidime and imipenem, this study showed B. pseudomallei had a gene that produced beta-lactamase enzyme and may be poorly effective in the use of beta-lactam drugs.
authors.forename: Marutpong
authors.initials: M
authors.lastname: Panya
authors.forename: Suthinee
authors.initials: S
authors.lastname: Thirat
authors.forename: Surasak
authors.initials: S
authors.lastname: Wanram
authors.forename: Pawana
authors.initials: P
authors.lastname: Panomket
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Nilsakul
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016957:Burkholderia pseudomallei / Q000187:drug effects*; D015780:Carbapenems / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008554:Melioidosis / Q000188:drug therapy* / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D013785:Thailand / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D015378:Imipenem; D002442:Ceftazidime; D001618:beta-Lactamases