title: Evaluation of antinociceptive and anti-inflammatory properties of methanolic crude extract of Lophopetalum javanicum (bark).
abstract: BACKGROUND
  The aim of the current study was to investigate the scientific basis of the traditional application of Lophopetalum javanicum for measuring anti-inflammatory and analgesic activity and phytochemical screening.
  
  
  METHODS
  Present study includes the preliminary screening of the phytochemical composition and in vivo analgesic and anti-inflammatory activity of methanolic extract of L. javanicum (MELJ). Hot-plate test and tail immersion method were used to investigate acute analgesic effects of L. javanicum, and the potency in inhibition of chronic inflammation in mice was tested by carrageenan-induced paw edema and formalin-induced edema method.
  
  
  RESULTS
  One hour after the administration of carrageenan, rat's paw was inflamed, and after treating it with 500 mg/kg dose, increase in the significant inhibitory effect on paw was observed. At the third hour after carrageenan injection, extreme inhibition (55.61%±0.015%; p<0.001) resulted by methanolic extract. By using hot plate method, it was found that L. javanicum increases pain tolerance time up to 17.89±0.079 min, whereas the compared standard's interval was 21.48±0.397 min. In tail immersion method, the pain threshold was 3.02±0.074 (p<0.001) at 400 mg/kg by L. javanicum at 90 min of experiment.
  
  
  CONCLUSIONS
  This study manifested that the methanolic extract of L. javanicum is efficient in inhibiting pain mediators to release, and conceivably, this report should get priority while searching for a new analgesic and anti-inflammatory agent.
authors.forename: Prawej
authors.initials: P
authors.lastname: Ansari
authors.forename: Sanjeeda Sarmin
authors.initials: SS
authors.lastname: Badhan
authors.forename: Shofiul
authors.initials: S
authors.lastname: Azam
authors.forename: Nasrin
authors.initials: N
authors.lastname: Sultana
authors.forename: Sabbir
authors.initials: S
authors.lastname: Anwar
authors.forename: Mohamed Sheikh
authors.initials: MS
authors.lastname: Mohamed Abdurahman
authors.forename: J M A
authors.initials: JM
authors.lastname: Hannan
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D002351:Carrageenan / Q000494:pharmacology; D029753:Celastraceae / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D000432:Methanol; D051379:Mice; D010146:Pain / Q000188:drug therapy; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D002351:Carrageenan; D000432:Methanol