title: Lactobacillus plantarum NCU116 Attenuates Cyclophosphamide-Induced Immunosuppression and Regulates Th17/Treg Cell Immune Responses in Mice.
abstract: The balance of T helper cells 17 (Th17)/regulatory T cells (Treg) plays a key role in maintaining a normal immune response. It is well-known that cyclophosphamide (CTX) applied at high dose often damages the immune system by inhibiting immune cell proliferation. In this study, the immunomodulating effects of Lactobacillus plantarum NCU116 in CTX-induced immunosuppression mice were investigated. Results showed that the levels of cytokines interleukin (IL)-17 and IL-21 were significantly increased after 10 days of treatment with a high dose of NCU116 (46.92 ± 4.28 and 119.92 ± 10.89, respectively) compared with the model group (36.20 ± 2.63, 61.00 ± 6.92, respectively), and the levels of cytokines IL-23 and TGF-β3 of the three NCU116 treatment groups were significantly higher than that of the model group (90.48 ± 6.33 and 140.45 ± 14.30, respectively) (p < 0.05) and close to 62 and 69% of the normal group's level (140.98 ± 14.74 and 266.95 ± 23.11, respectively) at 10 days. The bacterium was also found to increase the expression levels of Th17 immune response and Treg immune response specific transcription factors RORγt and Foxp3. In addition, the bacterium significantly increased the number of CD4(+)T cells and dendrtic cells (DCs) and up-regulated mRNA expression of Toll-like receptors (TLRs). These findings demonstrated that NCU116 has the potential ability to enhance intestinal mucosa immunity and regulate the Th17/Treg balance, which may be attributed to the TLR pathway in DCs.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.|School of Biological Sciences, The University of Auckland , Private Bag 92019, Auckland, New Zealand.
authors.forename: Deming
authors.initials: D
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Xie
mesh_terms: D000818:Animals; D003520:Cyclophosphamide / Q000009:adverse effects; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D007421:Intestine, Small / Q000187:drug effects / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000494:pharmacology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology*
chemical_list: D016207:Cytokines; D003520:Cyclophosphamide
keywords: Lactobacillus plantarum NCU116; Th17/Treg balance; Toll-like receptor; dendritic cells; immunosuppression