title: Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells.
abstract: The effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods. Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose (HG) growth conditions as compared to isoosmotic/normal glucose control (NG(⁎)) conditions. Unique protein expression changes at 2 h HG treatment were measured for 51 protein spots. These proteins could be broadly grouped into two categories: (1) proteins involved in cell survival/cell signaling and (2) proteins involved in stress response. Immunoblot experiments for a protein belonging to both categories, prohibitin (PHB), supported a trend for increased total expression as well as significant increases in an acidic PHB isoform. Additional studies confirmed the regulation of proteasomal subunit alpha-type 2 and the endoplasmic reticulum chaperone and oxidoreductase PDI (protein disulfide isomerase), suggesting altered ER protein folding capacity and proteasomal function in response to acute HG. We conclude that short term high glucose induces subtle changes in protein abundances suggesting posttranslational modifications and regulation of pathways involved in proteostasis.
authors.affiliation: Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Michelle T
authors.initials: MT
authors.lastname: Barati
authors.affiliation: Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA; Harvard Medical School, Boston, MA 02115, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Gould
authors.affiliation: Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA; Tuskegee University School of Veterinary Medicine, Tuskegee, AL 36088, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Salyer
authors.affiliation: Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Isaacs
authors.affiliation: Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Wilkey
authors.affiliation: Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Merchant
mesh_terms: D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D005947:Glucose / Q000633:toxicity*; D006706:Homeostasis; D006943:Hyperglycemia / Q000378:metabolism*; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism; D000091402:Prohibitins; D060066:Protein Interaction Maps; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: C000717332:Phb protein, rat; D000091402:Prohibitins; D011506:Proteins; D005947:Glucose
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