title: Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus).
abstract: Golden pompano is a commercially important marine fish that is widely cultured in China, Japan, and Southeast Asian countries but has been seriously threatened by pathogen. A novel cell line (TOGB) derived from the brain of golden pompano Trachinotus ovatus was established and characterized in this study. TOGB cell line showed high virus susceptibility, especially grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). As one of the most devastating viruses in marine fish aquaculture, nervous necrosis virus (NNV) causes high mortality rates exceeding 95% in severe outbreaks. Then, TOGB cell line was a useful tool for propagating viruses and provides a potentially valuable resource for the study of viral pathogenesis, the development of antiviral strategies. The TOGB cell lines showed potential application in environmental monitoring. The extracellular products from Vibrio anguillarum and Vibrio alginolyticus demonstrated cytotoxic effects in TOGB cells. TOGB cells grew most rapidly at 28°C, with an optimal concentration of 10% fetal bovine serum in L-15 medium. TOGB cells were diploid (2N = 54). The transfection efficiencies of TOGB cells were 8.6% at the 15th passage and 64.8% at the 45th passage, indicating that the cells are suitable for foreign gene expression.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Songwei
authors.initials: S
authors.lastname: Ni
authors.affiliation: State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources, College of Marine Science, Hainan University, Haikou, 570228, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Yepin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cai
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China. weisn@scsio.ac.cn.
authors.forename: Shina
authors.initials: S
authors.lastname: Wei
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China. qinqw@scsio.ac.cn.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Qin
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology*; D016923:Cell Death; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism; D005110:Extracellular Space / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007700:Kinetics; D008297:Male; D030381:Nodaviridae / Q000502:physiology / Q000648:ultrastructure; D010473:Perciformes / Q000378:metabolism*; D013696:Temperature; D014162:Transfection; D014779:Virus Replication
chemical_list: D049452:Green Fluorescent Proteins
keywords: Brain cell line; Trachinotus ovatus; Transfection efficiency; Virus susceptibility
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