title: Impacts of Rho kinase inhibitor Fasudil on Rho/ROCK signaling pathway in rabbits with optic nerve injury.
abstract: OBJECTIVE
  The aim of this study was to study the impacts of Rho kinase inhibitor Fasudil on expressions of Rho/ROCK signaling pathway associated genes in rabbits with optic nerve injury (ONI), and to explore the therapeutic mechanisms towards ONI.
  
  
  METHODS
  The rabbit ONI model was established, then the rabbits were divided into model group (treated with saline), control group (treated with dexamethasone, Dex), and intervention group (treated with Fasudil, Fas). The eyeball and optic nerve were sampled at 3, 7, 14 and 21 days after injury. The morphological changes of retina and optic nerve were observed. The expressions of RhoA, Caspase-3, Rock 2 and Nogo-A gene were determined by immunohistochemistry and real-time polymerase chain reaction (RT-PCR) methods.
  
  
  RESULTS
  At different time after injury, there were significant differences of RhoA, Caspase-3, Rock 2 and Nogo-A gene expression among three groups (P < 0.05).
  
  
  CONCLUSIONS
  After ONI, Fas can decrease the expression of Caspase-3 gene, and down-regulate the expressions of Nogo-A and Rock 2 gene. Therefore, it can treat ONI through affecting the Rho/ROCK signaling pathway.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830063, Xinjiang, P.R. China; Department of Neurosurgery, People's Hospital of Xinjiang Bazhou RegionKorla 841000, Xinjiang, P.R. China.
authors.forename: Jianglong
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Xinjiang Medical University Urumqi, 830000, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical University Urumqi 830063, Xinjiang, P.R. China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Luan
authors.affiliation: Department of Neurosurgery, People's Hospital of Xinjiang Bazhou Region Korla 841000, Xinjiang, P.R. China.
authors.forename: Xiepan
authors.initials: X
authors.lastname: Jing
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical University Urumqi 830063, Xinjiang, P.R. China.
authors.lastname: Maierab
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D020221:Optic Nerve Injuries / Q000201:enzymology* / Q000473:pathology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D047428:Protein Kinase Inhibitors; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D054460:rho-Associated Kinases; C049347:fasudil
keywords: Fasudil; Nogo-A; Rock 2; optic nerve injury
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