title: Electron probe X-ray microanalysis of boar and inobuta testes after the Fukushima accident.
abstract: We aimed to investigate the effect of chronic radiation exposure associated with the Fukushima Daiichi Nuclear Power Plant (FNPP) accident on the testes of boar and inobuta (a hybrid of Sus scrofa and Sus scrofa domestica). This study examined the contamination levels of radioactive caesium (Cs), especially (134)Cs and (137)Cs, in the testis of both boar and inobuta during 2012, after the Fukushima accident. Morphological analysis and electron-probe X-ray microanalysis (EPMA) were also undertaken on the testes. The (134)Cs and (137)Cs levels were 6430 ± 23 and 6820 ± 32 Bq/kg in the boar testes, and 755 ± 13 and 747 ± 17 Bq/kg in the inobuta testes, respectively. The internal and external exposure of total (134)Cs and (137)Cs in the boar testes were 47.1 mGy and 176.2 mGy, respectively, whereas in the inobuta testes, these levels were 6.09 mGy and 59.8 mGy, respectively. Defective spermatogenesis was not detected by the histochemical analysis of radiation-exposed testes for either animal. In neither animal were Cs molecules detected, using EPMA. In conclusion, we showed that adverse radiation-induced effects were not detected in the examined boar and inobuta testes following the chronic radiation exposure associated with the FNPP accident.
authors.affiliation: Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yamashiro
authors.affiliation: Research Centre for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Abe
authors.affiliation: Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Gohei
authors.initials: G
authors.lastname: Hayashi
authors.affiliation: Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Urushihara
authors.affiliation: Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuwahara
authors.affiliation: Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: School of Food, Agricultural and Environmental Sciences, Miyagi University, Sendai, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Science, Tohoku University, Sendai, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kino
authors.affiliation: Graduate School of Agricultural Sciences, Tohoku University, Sendai, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Bin
authors.initials: B
authors.lastname: Tong
authors.affiliation: Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Sachio
authors.initials: S
authors.lastname: Takino
authors.affiliation: Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Yukou
authors.initials: Y
authors.lastname: Sugano
authors.affiliation: Institute of Agriculture, Department of Biological Production, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sugimura
authors.affiliation: Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Graduate School of Agricultural Sciences, Tohoku University, Sendai, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Isogai
authors.affiliation: Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan fukumoto@idac.tohoku.ac.jp.
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukumoto
mesh_terms: D000818:Animals; D002588:Cesium Radioisotopes / Q000032:analysis; D004577:Electron Probe Microanalysis; D062406:Fukushima Nuclear Accident; D008297:Male; D011834:Radiation Monitoring; D013552:Swine; D013737:Testis / Q000737:chemistry* / Q000528:radiation effects*
chemical_list: D002588:Cesium Radioisotopes
keywords: EPMA; Fukushima Daiichi Nuclear Power Plant; boar; inobuta; radioactive caesium; testis
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