title: Cilostazol Improves Developmental Competence of Pig Oocytes by Increasing Intraoocyte Cyclic Adenosine Monophosphate Level and Delaying Meiotic Resumption.
abstract: Cilostazol (CLZ) is a cyclic adenosine monophosphate (cAMP) modulator that influences the steady state of the meiotic stage. This study was conducted to determine the effects of CLZ treatment during in vitro maturation (IVM) on developmental competence of pig oocytes. Immature oocytes were exposed to 0 (control), 0.5, 2 and 4 μm CLZ during the first 22 h of IVM. Nuclear maturation, intraoocyte glutathione content and embryo cleavage after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) were not influenced by CLZ at any concentrations. However, 4 μm CLZ significantly (p < 0.05) improved blastocyst formation after PA (52.1% vs 38.7-46.0%) and SCNT relative to other concentrations (40.8% vs 25.0-30.7%). The mean cell numbers of SCNT blastocysts were significantly increased by 4 μm CLZ compared to the control (42.6 cells vs 35.3 cells/blastocyst). CLZ treatment significantly increased the intraoocyte cAMP level and effectively arrested oocytes at the germinal vesicle (GV) and GV break down stages compared to the control (74.5% vs 45.4%). Our results demonstrated that improved developmental competence of PA and SCNT pig embryos occurred via better synchronization of nuclear and cytoplasmic maturation induced by increased cAMP and delayed meiotic resumption after CLZ treatment.
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors.forename: F
authors.initials: F
authors.lastname: Elahi
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors.forename: B
authors.initials: B
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.affiliation: Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Hyun
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.|Institute of Veterinary Science, Kangwon National University, Chuncheon, Korea.
authors.forename: E
authors.initials: E
authors.lastname: Lee
mesh_terms: D000818:Animals; D000077407:Cilostazol; D000242:Cyclic AMP / Q000378:metabolism*; D046149:Embryo Culture Techniques / Q000662:veterinary; D008540:Meiosis / Q000187:drug effects* / Q000502:physiology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D058987:Phosphodiesterase 3 Inhibitors / Q000494:pharmacology; D013552:Swine / Q000196:embryology; D013777:Tetrazoles / Q000494:pharmacology*
chemical_list: D058987:Phosphodiesterase 3 Inhibitors; D013777:Tetrazoles; D000242:Cyclic AMP; D000077407:Cilostazol