title: [ANTI-Hsp60 ANTIBODIES IN ARTERIAL HYPERTENTION DIFERENT DEGREE OF SEVERITY].
abstract: More than 12.1 million people with hypertension (32.2% of the adult population) were registered in Ukraine according to the official statistics on 1 January 2011. The etiopathogenesis of AH is not fully established. Hsp60 is the molecular chaperon/chaperonin, and it's expression significantly increases in response to different kinds of stress (emotional stress, infections, smoking etc). Elevated blood pressure is a mechanical stress to the endothelium and it can induce expression of heat-shock protein 60 (Hsp60) on the endothelial cell surface. Endothelial cells in the vessel wall can be damaged by (auto) immune reactions to Hsp60 present on the cell surface. Elevation of anti-Hsp60 in the circulation is associated with the presence and severity of coronary heart disease, atherosclerosis development, pathological changes in the small vessels of the brain etc etc. Specificity of the anti-Hsp60 antibodies and their role in the pathogenesis of AH has not been established. The aim of this work was to identify the level of anti-Hsp60 antibodies in the sera of patients with AH. 128 patients with AH were examined. To define level of anti-Hsp60 antibodies the sera 39 patients with AH, including 12 clinically healthy individuals (the family history are included the AH cases)--1 group, 19 patients with stage 2--2 group and 8 patients with stage 3--3 group were examined. The control group included 112 blood donors. Anti-Hsp60 antibodies in sera were determined by ELISA and immunobloting (Western-blotting). Recombinant piotein GroEL Escherihia coli (prokaryotic homologue of human Hsp60) and human Hsp60 were used as antigens. Average of levels of antibodies against GroEL and human Hsp60 in the serum of all groups twice exeeded the value of the control (P < 0.001). Antibodies to prokaryotic Hsp60 were prevailed in patients with AH. The seropositive serum to Hsp60 were detectived in patients, that had the risk of the AH complications by ELISA and immunoblotting. In addition, highly reactive IgG anti-Hsp60 antibodies purified by affinity chromatography from human sera of patients with AH recognized GroEL and human Hsp60 in immunoblotting. Elevated levels of anti-Hsp60 antibody in sera of patients with AH stage 3 correlated with pronounced changes in the target organs such as a massive recurrent hemorrhage into the retina, acute ischemic stroke, cardiosclerosis and angionephrosclerosis. It may indicate the involvement of anti-Hsp60 antibodies in the development of the target organ damage.
authors.forename: L F
authors.initials: LF
authors.lastname: Yakovenko
authors.forename: Y V
authors.initials: YV
authors.lastname: Smalyuk
authors.forename: L M
authors.initials: LM
authors.lastname: Kapustyan
authors.forename: S A
authors.initials: SA
authors.lastname: Chornyy
authors.forename: A P
authors.initials: AP
authors.lastname: Pogrebnaya
authors.forename: V N
authors.initials: VN
authors.lastname: Granich
authors.forename: A S
authors.initials: AS
authors.lastname: Dobrochod
authors.forename: E A
authors.initials: EA
authors.lastname: Torbas
authors.forename: A D
authors.initials: AD
authors.lastname: Radchenko
authors.forename: Yu N
authors.initials: YN
authors.lastname: Sirenko
authors.forename: L L
authors.initials: LL
authors.lastname: Sidorik
mesh_terms: D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000096:biosynthesis*; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D040941:Heredity / Q000276:immunology*; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012720:Severity of Illness Index
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D018834:Chaperonin 60; C060293:HSPD1 protein, human; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins