title: Three new steroidal saponins from Helleborus thibetanus.
abstract: Three new steroidal saponins including two spirostanol glycosides (1-2) and one furostanol glycoside 1-sulphate (3) were isolated from the dried roots and rhizomes of Helleborus thibetanus. Structures of the compounds were determined on the basis of extensive use of 1-D and 2-D NMR experiments, together with HR-ESI-MS and IR measurements, as well as the results of acid hydrolysis. Compounds 1-2 represented steroidal saponins with an unusual substitution pattern, which possessed a double bond at C-25 and were glycosylated at 1-OH.
authors.affiliation: a Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin , P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin , P.R. China.|b Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin , P.R. China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Su
authors.affiliation: a Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin , P.R. China.|c Pharmaceutical Engineering Department, School of Biological Science and Technology , University of Jinan , Jinan , P.R. China.
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Yang
mesh_terms: D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D039322:Helleborus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D006027:Glycosides; D012503:Saponins; D013150:Spirostans; D013261:Sterols; C574436:furostanol glycoside
keywords: Helleborus; Ranunculaceae; furostanol; spirostanol; steroidal saponins