title: The wheat homolog of putative nucleotide-binding site-leucine-rich repeat resistance gene TaRGA contributes to resistance against powdery mildew.
abstract: Powdery mildew, one of the most destructive wheat diseases worldwide, is caused by Blumeria graminis f. sp. tritici (Bgt), a fungal species with a consistently high mutation rate that makes individual resistance (R) genes ineffective. Therefore, effective resistance-related gene cloning is vital for breeding and studying the resistance mechanisms of the disease. In this study, a putative nucleotide-binding site-leucine-rich repeat (NBS-LRR) R gene (TaRGA) was cloned using a homology-based cloning strategy and analyzed for its effect on powdery mildew disease and wheat defense responses. Real-time reverse transcription-PCR (RT-PCR) analyses revealed that a Bgt isolate 15 and salicylic acid stimulation significantly induced TaRGA in the resistant variety. Furthermore, the silencing of TaRGA in powdery mildew-resistant plants increased susceptibility to Bgt15 and prompted conidia propagation at the infection site. However, the expression of TaRGA in leaf segments after single-cell transient expression assay highly increased the defense responses to Bgt15 by enhancing callose deposition and phenolic autofluorogen accumulation at the pathogen invading sites. Meanwhile, the expression of pathogenesis-related genes decreased in the TaRGA-silenced plants and increased in the TaRGA-transient-overexpressing leaf segments. These results implied that the TaRGA gene positively regulates the defense response to powdery mildew disease in wheat.
authors.affiliation: College of Life Science, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.|National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Wang
authors.affiliation: National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mei
authors.affiliation: National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China. hsdong@njau.edu.cn.
authors.forename: Hansong
authors.initials: H
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D000090263:Leucine-Rich Repeat Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D011506:Proteins / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D004718:Saccharomycetales / Q000254:growth & development / Q000472:pathogenicity; D020156:Salicylic Acid / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins; D011994:Recombinant Proteins; D020156:Salicylic Acid
keywords: Functional identification; NBS-LRR; Resistance gene; Wheat
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