title: In situ plasma fabrication of ceramic-like structure on polymeric implant with enhanced surface hardness, cytocompatibility and antibacterial capability.
abstract: Polymeric materials are commonly found in orthopedic implants due to their unique mechanical properties and biocompatibility but the poor surface hardness and bacterial infection hamper many biomedical applications. In this study, a ceramic-like surface structure doped with silver is produced by successive plasma implantation of silicon (Si) and silver (Ag) into the polyamine 66 (PA66) substrate. Not only the surface hardness and elastic modulus are greatly enhanced due to the partial surface carbonization and the ceramic-like structure produced by the reaction between energetic Si and the carbon chain of PA66, but also the antibacterial activity is improved because of the combined effects rendered by Ag and SiC structure. Furthermore, the modified materials which exhibit good cytocompatibility upregulate bone-related genes and proteins expressions of the contacted bone mesenchymal stem cells (BMSCs). For the first time, it explores out that BMSCs osteogenesis on the antibacterial ceramic-like structure is mediated via the iNOS and nNOS signal pathways. The results reveal that in situ plasma fabrication of an antibacterial ceramic-like structure can endow PA66 with excellent surface hardness, cytocompatibility, as well as antibacterial capability.
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Haigang
authors.initials: H
authors.lastname: Shi
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Gexia
authors.initials: G
authors.lastname: Wang
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Pingli
authors.initials: P
authors.lastname: Wang
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Ji
authors.affiliation: Department of Physics & Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000517:prevention & control; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology; D002460:Cell Line; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology; D055119:Elastic Modulus; D006244:Hardness; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D019736:Prostheses and Implants; D012825:Silicon / Q000737:chemistry* / Q000494:pharmacology; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011073:Polyamines; D011108:Polymers; D012834:Silver; D012825:Silicon
keywords: antibacterial activity; ceramic-like structure; osteogenic activity; surface hardness