title: In vivo dermal absorption of pyrethroid pesticides in the rat.
abstract: Exposure to pyrethroid pesticides is a potential cause for concern. The objective of this study was to examine the in vivo dermal absorption of bifenthrin, deltamethrin, and permethrin in the rat. Dorsal hair on adult male Long-Evans rats was removed. The next day, the skin was dosed with 1750 nmol (312.5 nmol/cm(2)) of radiolabeled (5 µCi) bifenthrin, deltamethrin, or permethrin in acetone. A nonoccluding plastic cover was glued over the dosing site. The animals were placed in metabolism cages to collect excreta. At 24 h postdosing, the skin was washed with soap and water, and rats in one group were euthanized and their tissues were collected. The skin was removed and tape stripped. The remaining animals were returned to the metabolism cages after the wash for 4 d. These rats were then euthanized and handled as already described. Excreta, wash, tape strips, tissues, and carcass were analyzed for pyrethroid-derived radioactivity. The wash and tape strips removed >50% of the dose and skin retained 9-24%. Cumulative radioactivity in excreta was 0.5-7% at 24 h and 3-26% at 120 h. Radioactivity in tissues was <0.3% of the dose, while carcass retained 2 to 5%. Assuming absorption equals cumulative recovery in skin (washed and tape stripped), excreta, tissues, and carcass, absorption was permethrin ~ bifenthrin > deltamethrin at 24 h and permethrin > deltamethrin > bifenthrin at 120 h. Using the parallelogram approach with published in vitro data, human dermal absorption of these pyrethroids was estimated to be <10% of the dose.
authors.affiliation: a U.S. Environmental Protection Agency , Research Triangle Park, North Carolina , USA.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Hughes
authors.affiliation: a U.S. Environmental Protection Agency , Research Triangle Park, North Carolina , USA.
authors.forename: Brenda C
authors.initials: BC
authors.lastname: Edwards
mesh_terms: D000818:Animals; D001822:Body Burden; D005243:Feces / Q000737:chemistry; D007306:Insecticides / Q000493:pharmacokinetics* / Q000652:urine; D007553:Isotope Labeling; D008297:Male; D009570:Nitriles / Q000493:pharmacokinetics / Q000652:urine; D026023:Permethrin / Q000493:pharmacokinetics / Q000652:urine; D011722:Pyrethrins / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D020318:Rats, Long-Evans; D012869:Skin Absorption; D014018:Tissue Distribution
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D026023:Permethrin; C099952:bifenthrin