title: Inactivation of Escherichia coli, Listeria monocytogenes, and Salmonella Enteritidis by Cymbopogon citratus D.C. Stapf. Essential Oil in Pineapple Juice.
abstract: In the present study, the efficacy of Cymbopogon citratus D.C. Stapf. essential oil (CCEO) to provoke a 5-log CFU/ml (5-log) inactivation in a mixed composite of Escherichia coli, Listeria monocytogenes, and Salmonella enterica serovar Enteritidis in pineapple (Ananas comosus (L.) Merril) juice (4°C) was assessed. Moreover, the effects of CCEO on the physicochemical and sensory quality parameters of pineapple juice were evaluated. The MIC of CCEO was 5 μl/ml against the composite mix examined. For L. monocytogenes and E. coli inoculated in juice containing CCEO (5, 2.5, and 1.25 μl/ml), a ≥5-log reduction was detected after 15 min of exposure. This same result was obtained for Salmonella Enteritidis incubated alone in pineapple juice containing CCEO at 5 and 2.5 μl/ml. Overall, Salmonella Enteritidis was the most tolerant and L. monocytogenes was the most sensitive to CCEO. The physicochemical properties (pH, titratable acidic [citric acid per 100 g], and soluble solids) of pineapple juice containing CCEO (2.5 and 1.25 μl/ml) were maintained. Juice containing CCEO (2.5 and 1.25 μl/ml) exhibited similar scores for odor, appearance, and viscosity compared with juice without CCEO. However, unsatisfactory changes in taste and aftertaste were observed in juices containing CCEO. These results suggest that CCEO could be used as an alternative antimicrobial compound to ensure the safety of pineapple juice, although CCEO at the tested concentrations negatively impacted its taste. Therefore, further studies are needed to determine the balance between microbial safety and taste acceptability of pineapple juice containing CCEO.
authors.affiliation: Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paráıba, João Pessoa, 58051900, Brazil.
authors.forename: Caroline Junqueira Barcellos
authors.initials: CJ
authors.lastname: Leite
authors.affiliation: Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paráıba, João Pessoa, 58051900, Brazil.
authors.forename: Jossana Pereira
authors.initials: JP
authors.lastname: de Sousa
authors.affiliation: Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paráıba, João Pessoa, 58051900, Brazil.
authors.forename: José Alberto da Costa
authors.initials: JA
authors.lastname: Medeiros
authors.affiliation: Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paráıba, João Pessoa, 58051900, Brazil.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: da Conceição
authors.affiliation: Laboratory of Genetics of Microorganisms, Department of Molecular Biology, Center of Exact and Natural Sciences, Federal University of Paráıba, João Pessoa, 58051900, Brazil.
authors.forename: Vivyanne
authors.initials: V
authors.lastname: dos Santos Falcão-Silva
authors.affiliation: Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paráıba, João Pessoa, 58051900, Brazil. evandroleitesouza@ccs.ufpb.br.
authors.forename: Evandro Leite
authors.initials: EL
authors.lastname: de Souza
mesh_terms: D031230:Ananas / Q000737:chemistry / Q000382:microbiology; D001628:Beverages / Q000382:microbiology*; D031743:Cymbopogon / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005503:Food Additives / Q000302:isolation & purification / Q000494:pharmacology*; D005506:Food Contamination / Q000032:analysis; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development
chemical_list: D005503:Food Additives; D009822:Oils, Volatile; D010936:Plant Extracts