title: Preparation of Cu(2+)-mediated magnetic imprinted polymers for the selective sorption of bovine hemoglobin.
abstract: In this work, a novel Cu(2+)-mediated core-shell bovine hemoglobin imprinted superparamagnetic polymers were synthesized. First, carboxyl group directly-functionalized Fe3O4 nanoparticles were produced by a facile one-pot hydrothermal method. Next, copper ions were introduced to chelate with carboxyl groups and further bonded with template bovine hemoglobin as co-functional monomer. Then, functional monomers 3-aminopropyltriethoxylsilane and octyltrimethoxysilane were adopted to form the thin polymer layers. Finally, after removal of the templates, the imprinting shells with specific recognition cavities for bovine hemoglobin were obtained on Fe3O4 nanoparticles. The resultant molecularly imprinted polymers have high adsorption capacity and satisfactory selectivity for bovine hemoglobin with the help of copper ions. The obtained magnetic nanomaterials were characterized by transmission electron microscopy, Fourier-transform infrared spectra, X-ray diffraction, and vibrating sample magnetometer. The measurements demonstrated that the as-synthesized nanomaterials exhibited good dispersion, high crystallinity, and satisfactory superparamagnetic properties. The feasibility of this method was further confirmed by using the imprinted nanomaterials to specifically extract bovine hemoglobin from real bovine blood samples.
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: ruixiagao@mail.xjtu.edu.cn.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Gao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Cui
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Gaiyan
authors.initials: G
authors.lastname: He
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: tyh57@mail.xjtu.edu.cn.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D013696:Temperature
chemical_list: D002614:Chelating Agents; D006454:Hemoglobins; D058185:Magnetite Nanoparticles; D011108:Polymers; D003300:Copper
keywords: Copper ions; Magnetic separation; Molecularly imprinted polymers