title: The Influence of C-Ions and X-rays on Human Umbilical Vein Endothelial Cells.
abstract: Damage to the endothelium of blood vessels, which may occur during radiotherapy, is discussed as a potential precursor to the development of cardiovascular disease. We thus chose human umbilical vein endothelial cells as a model system to examine the effect of low- and high-linear energy transfer (LET) radiation. Cells were exposed to 250 kV X-rays or carbon ions (C-ions) with the energies of either 9.8 MeV/u (LET = 170 keV/μm) or 91 MeV/u (LET = 28 keV/μm). Subculture of cells was performed regularly up to 46 days (~22 population doublings) post-irradiation. Immediately after exposure, cells were seeded for the colony forming assay. Additionally, at regular intervals, mitochondrial membrane potential (MMP) (JC-1 staining) and cellular senescence (senescence-associated β-galactosidase staining) were assessed. Cytogenetic damage was investigated by the micronucleus assay and the high-resolution multiplex fluorescence in situ hybridization (mFISH) technique. Analysis of radiation-induced damage shortly after exposure showed that C-ions are more effective than X-rays with respect to cell inactivation or the induction of cytogenetic damage (micronucleus assay) as observed in other cell systems. For 9.8 and 91 MeV/u C-ions, relative biological effectiveness values of 2.4 and 1.5 were obtained for cell inactivation. At the subsequent time points, the number of micronucleated cells decreased to the control level. Analysis of chromosomal damage by mFISH technique revealed aberrations frequently involving chromosome 13 irrespective of dose or radiation quality. Disruption of the MMP was seen only a few days after exposure to X-rays or C-ions. Cellular senescence was not altered by radiation at any time point investigated. Altogether, our data indicate that shortly after exposure C-ions were more effective in damaging endothelial cells than X-rays. However, late damage to endothelial cells was not found for the applied conditions and endpoints.
authors.affiliation: Department of Biophysics, GSI Helmholtz Centre for Heavy Ion Research , Darmstadt , Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Helm
authors.affiliation: Department of Biophysics, GSI Helmholtz Centre for Heavy Ion Research , Darmstadt , Germany.
authors.forename: Ryonfa
authors.initials: R
authors.lastname: Lee
authors.affiliation: Department of Biophysics, GSI Helmholtz Centre for Heavy Ion Research, Darmstadt, Germany; Department of Condensed Matter Physics, Technical University of Darmstadt, Darmstadt, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Durante
authors.affiliation: Department of Biophysics, GSI Helmholtz Centre for Heavy Ion Research , Darmstadt , Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Ritter
keywords: carbon ion therapy; carbon ions; cardiovascular disease; chromosome 13; endothelial cells; high-LET radiation; micronucleus formation; senescence-associated β-galactosidase
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