title: Exercise-Induced Changes in Caveolin-1, Depletion of Mitochondrial Cholesterol, and the Inhibition of Mitochondrial Swelling in Rat Skeletal Muscle but Not in the Liver.
abstract: The reduction in cholesterol in mitochondria, observed after exercise, is related to the inhibition of mitochondrial swelling. Caveolin-1 (Cav-1) plays an essential role in the regulation of cellular cholesterol metabolism and is required by various signalling pathways. Therefore, the aim of this study was to investigate the effect of prolonged swimming on the mitochondrial Cav-1 concentration; additionally, we identified the results of these changes as they relate to the induction of changes in the mitochondrial swelling and cholesterol in rat skeletal muscle and liver. Male Wistar rats were divided into a sedentary control group and an exercise group. The exercised rats swam for 3 hours and were burdened with an additional 3% of their body weight. After the cessation of exercise, their quadriceps femoris muscles and livers were immediately removed for experimentation. The exercise protocol caused an increase in the Cav-1 concentration in crude muscle mitochondria; this was related to a reduction in the cholesterol level and an inhibition of mitochondrial swelling. There were no changes in rat livers, with the exception of increased markers of oxidative stress in mitochondria. These data indicate the possible role of Cav-1 in the adaptive change in the rat muscle mitochondria following exercise.
authors.affiliation: Department of Bioenergetics and Physiology of Exercise, Medical University of Gdansk, 80-210 Gdansk, Poland; Department of Bioenergetics and Nutrition, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland.
authors.forename: Damian Jozef
authors.initials: DJ
authors.lastname: Flis
authors.affiliation: Department of Bioenergetics and Nutrition, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland.
authors.forename: Robert Antoni
authors.initials: RA
authors.lastname: Olek
authors.affiliation: Department of Physiotherapy, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland.
authors.forename: Jan Jacek
authors.initials: JJ
authors.lastname: Kaczor
authors.affiliation: Department of Bioenergetics and Nutrition, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Rodziewicz
authors.affiliation: Department of Physiotherapy, Medical University of Gdansk, 80-210 Gdansk, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Halon
authors.affiliation: Department of Biochemistry, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland.
authors.forename: Jedrzej
authors.identifier: 0000-0002-7871-6469
authors.initials: J
authors.lastname: Antosiewicz
authors.affiliation: Department of Medical Chemistry, Medical University of Gdansk, 80-210 Gdansk, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Wozniak
authors.affiliation: School of Pharmacy, University of Camerino, 62032 Camerino, Italy.
authors.forename: Rosita
authors.initials: R
authors.lastname: Gabbianelli
authors.affiliation: Department of Bioenergetics and Nutrition, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland.
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Ziolkowski
mesh_terms: D000818:Animals; D001835:Body Weight; D051242:Caveolin 1 / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D008933:Mitochondrial Swelling; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D052097:Quadriceps Muscle / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D057185:Sedentary Behavior; D015398:Signal Transduction; D013550:Swimming
chemical_list: C495537:Cav1 protein, rat; D051242:Caveolin 1; D017382:Reactive Oxygen Species; D002784:Cholesterol
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