title: Lignocellulosic ethanol production by starch-base industrial yeast under PEG detoxification.
abstract: Cellulosic ethanol production from lignocellulosic biomass offers a sustainable solution for transition from fossil based fuels to renewable alternatives. However, a few long-standing technical challenges remain to be addressed in the development of an economically viable fermentation process from lignocellulose. Such challenges include the needs to improve yeast tolerance to toxic inhibitory compounds and to achieve high fermentation efficiency with minimum detoxification steps after a simple biomass pretreatment. Here we report an in-situ detoxification strategy by PEG exo-protection of an industrial dry yeast (starch-base). The exo-protected yeast cells displayed remarkably boosted vitality with high tolerance to toxic inhibitory compounds, and with largely improved ethanol productivity from crude hydrolysate derived from a pretreated lignocellulose. The PEG chemical exo-protection makes the industrial S. cerevisiae yeast directly applicable for the production of cellulosic ethanol with substantially improved productivity and yield, without of the need to use genetically modified microorganisms.
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Liaoyuan
authors.initials: L
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Peifang
authors.initials: P
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Z Conrad
authors.initials: ZC
authors.lastname: Zhang
mesh_terms: D018533:Biomass; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism; D013213:Starch / Q000737:chemistry
chemical_list: C036909:lignocellulose; D000431:Ethanol; D011092:Polyethylene Glycols; D013213:Starch; D008031:Lignin
references.citation: Appl Microbiol Biotechnol. 2006 Nov;73(1):27-36
references.pmid: 17028874
references.citation: Bioresour Technol. 2009 Jan;100(1):10-8
references.pmid: 18599291
references.citation: Biotechnol Biofuels. 2013 Oct 20;6(1):151
references.pmid: 24139317
references.citation: Biotechnol Bioeng. 2007 Apr 1;96(5):862-70
references.pmid: 16865734
references.citation: Sci Rep. 2015;5:9593
references.pmid: 25901788
references.citation: Environ Health Perspect. 1990 Jul;87:213-8
references.pmid: 2176586
references.citation: Biotechnol Bioeng. 2006 Apr 20;93(6):1196-206
references.pmid: 16470880
references.citation: Appl Microbiol Biotechnol. 2004 Nov;66(1):10-26
references.pmid: 15300416
references.citation: Biotechnol Bioeng. 1999 Apr 5;63(1):46-55
references.pmid: 10099580
references.citation: Science. 2006 Jan 27;311(5760):506-8
references.pmid: 16439656
references.citation: Appl Microbiol Biotechnol. 2009 Mar;82(4):625-38
references.pmid: 19184597
references.citation: Bioresour Technol. 2011 Dec;102(23):10868-74
references.pmid: 21983414
references.citation: Trends Biotechnol. 2014 Jan;32(1):46-53
references.pmid: 24231155
references.citation: Bioresour Technol. 2002 May;83(1):1-11
references.pmid: 12058826
references.citation: Biotechnol Bioeng. 2013 Oct;110(10):2616-23
references.pmid: 23613173
references.citation: Biotechnol Biofuels. 2011 Nov 10;4(1):49
references.pmid: 22074982
references.citation: FEMS Yeast Res. 2012 Mar;12(2):171-82
references.pmid: 22136139
references.citation: Bioresour Technol. 2009 Dec;100(24):6378-84
references.pmid: 19683434
references.citation: Appl Biochem Biotechnol. 2005 Spring;121-124:923-34
references.pmid: 15930571
references.citation: Biotechnol Biofuels. 2013 Jan 28;6(1):16
references.pmid: 23356676
references.citation: Nat Commun. 2013;4:2580
references.pmid: 24105024
references.citation: J Appl Microbiol. 2001 Feb;90(2):208-15
references.pmid: 11168723
references.citation: Biotechnol Adv. 2008 Jan-Feb;26(1):89-105
references.pmid: 17964107
references.citation: Bioresour Technol. 2014 Jul;164:331-7
references.pmid: 24874873
references.citation: J Biosci Bioeng. 2008 Aug;106(2):128-33
references.pmid: 18804054
references.citation: Bioresour Technol. 2013 Apr;133:455-61
references.pmid: 23454802
references.citation: Biotechnol Lett. 2010 Nov;32(11):1655-61
references.pmid: 20574836