title: Photoinhibition and photoinhibition-like damage to the photosynthetic apparatus in tobacco leaves induced by pseudomonas syringae pv. Tabaci under light and dark conditions.
abstract: BACKGROUND
  Pseudomonas syringae pv. tabaci (Pst), which is the pathogen responsible for tobacco wildfire disease, has received considerable attention in recent years. The objective of this study was to clarify the responses of photosystem I (PSI) and photosystem II (PSII) to Pst infection in tobacco leaves.
  
  
  RESULTS
  The net photosynthetic rate (Pn) and carboxylation efficiency (CE) were inhibited by Pst infection. The normalized relative variable fluorescence at the K step (W k) and the relative variable fluorescence at the J step (V J) increased while the maximal quantum yield of PSII (F v/F m) and the density of Q A-reducing PSII reaction centers per cross section (RC/CSm) decreased, indicating that the reaction centers, and the donor and acceptor sides of PSII were all severely damaged after Pst infection. The PSI activity decreased as the infection progressed. Furthermore, we observed a considerable overall degradation of PsbO, D1, PsaA proteins and an over-accumulation of reactive oxygen species (ROS).
  
  
  CONCLUSIONS
  Photoinhibition and photoinhibition-like damage were observed under light and dark conditions, respectively, after Pst infection of tobacco leaves. The damage was greater in the dark. ROS over-accumulation was not the primary cause of the photoinhibition and photoinhibition-like damage. The PsbO, D1 and PsaA proteins appear to be the targets during Pst infection under light and dark conditions.
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China. 819296624@qq.com.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Cheng
authors.affiliation: State Key Lab of Crop Biology, College of Life Sciences, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, China. zhangzishantaian@163.com.
authors.forename: Zi-Shan
authors.initials: ZS
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China. 290216154@qq.com.
authors.forename: Xing-Bin
authors.initials: XB
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China. 82191513@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China. sungy@vip.sina.com.
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China. Fred.Chow@anu.edu.au.|Division of Plant Science, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Acton, ACT 2601, Australia. Fred.Chow@anu.edu.au.
authors.forename: Wah Soon
authors.initials: WS
authors.lastname: Chow
mesh_terms: D003624:Darkness; D008027:Light; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D044224:Pseudomonas syringae / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology
chemical_list: D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D017382:Reactive Oxygen Species
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