title: Effects of triclosan on host response and microbial biomarkers during experimental gingivitis.
abstract: This exploratory randomized, controlled clinical trial sought to evaluate anti-inflammatory and -microbial effects of triclosan during experimental gingivitis as assessed by host response biomarkers and biofilm microbial pathogens.
  
  
  
  Thirty participants were randomized to triclosan or control dentifrice groups who ceased homecare for 21 days in an experimental gingivitis (EG) protocol. Plaque and gingival indices and saliva, plaque, and gingival crevicular fluid (GCF) were assessed/collected at days 0, 14, 21 and 35. Levels and proportions of 40 bacterial species from plaque samples were determined using checkerboard DNA-DNA hybridization. Ten biomarkers associated with inflammation, matrix degradation, and host protection were measured from GCF and saliva and analysed using a multiplex array. Participants were stratified as "high" or "low" responders based on gingival index and GCF biomarkers and bacterial biofilm were combined to generate receiver operating characteristic curves and predict gingivitis susceptibility.
  
  
  
  No differences in mean PI and GI values were observed between groups and non-significant trends of reduction of host response biomarkers with triclosan treatment. Triclosan significantly reduced levels of A. actinomycetemcomitans and P. gingivalis during induction of gingivitis.
  
  
  
  Triclosan reduced microbial levels during gingivitis development (ClinicalTrials.gov NCT01799226).
authors.affiliation: Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Brooke A
authors.initials: BA
authors.lastname: Pancer
authors.affiliation: Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Kott
authors.affiliation: Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, MI, USA.
authors.forename: James V
authors.initials: JV
authors.lastname: Sugai
authors.affiliation: Colgate-Palmolive Technology Center, Piscataway, NJ, USA.
authors.forename: Fotinos S
authors.initials: FS
authors.lastname: Panagakos
authors.affiliation: Biostatistics Department, School of Public Health, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Braun
authors.affiliation: Department of Periodontology, School of Dentistry, University of North Carolina, Chapel Hill, NC, USA.|Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA.
authors.forename: Ricardo P
authors.initials: RP
authors.lastname: Teles
authors.affiliation: Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, MI, USA.
authors.forename: William V
authors.identifier: 0000-0002-7102-9746
authors.initials: WV
authors.lastname: Giannobile
authors.affiliation: Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Janet S
authors.initials: JS
authors.lastname: Kinney
mesh_terms: D000891:Anti-Infective Agents, Local; D015415:Biomarkers; D003773:Dental Plaque; D003774:Dental Plaque Index; D003802:Dentifrices; D004311:Double-Blind Method; D005891:Gingivitis; D006801:Humans; D010512:Periodontal Index; D014260:Triclosan
chemical_list: D000891:Anti-Infective Agents, Local; D015415:Biomarkers; D003802:Dentifrices; D014260:Triclosan
keywords: anti-inflammatory agents; bacteria; dental plaque/microbiology; dentifrices; gingivitis; inflammation mediators/analysis