title: Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus).
abstract: BACKGROUND
  The liver plays important roles in nutrient metabolism, detoxification and immunity. Enterococcus faecium (E. faecium) is a probiotic that has been shown to have positive effects on broiler production. However, its molecular effects on liver metabolism have not been characterized. This study aims to further identify the biological roles of E. faecium by characterizing the hepatic proteomic changes of broilers (Gallus gallus) fed E. faecium using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) and mass spectrometry (MS).
  
  
  RESULTS
  Thirty-three proteins (50 protein spots) involved in nutrient metabolism, immunity and the antioxidant system were shown to be differentially expressed in the liver of broilers fed E. faecium than from birds not fed the probiotic. The biological processes of sulphur amino acids, vitamin and cellular hormone metabolism, sulphur compound biosynthesis and protein tetramerization were enhanced in the liver of broilers fed E. faecium. However, proteins involved in calcium ion flux, cell redox homeostasis and platelet activation related to hepatic immune responses were down-regulated in broilers fed E. faecium. These results indicate that the supplementation of poultry feed with E. faecium may alter the partitioning of nutrients and promote optimal nutrient utilization.
  
  
  CONCLUSIONS
  This study assists in unraveling the molecular effects of the dietary probiotic, E. faecium, in the liver of broiler chickens. It shows that the probiotic improves the metabolism of nutrients and decreases inflammatory responses. Our findings extend previous knowledge of the mechanism of dietary probiotic action and provide new findings for research and future probiotic development.
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. zhengaijuan@caas.cn.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. luojianjie521@163.com.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. mengkun@caas.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Meng
authors.affiliation: Key Laboratory of Pollinating Insect Biology of Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, 100093, People's Republic of China. apislijk@163.com.
authors.forename: Jianke
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Agriculture and Food Sciences, University of Queensland, Gatton, QLD, 4343, Australia. w.bryden@uq.edu.au.
authors.forename: Wayne L
authors.initials: WL
authors.lastname: Bryden
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. changwenhuan@caas.cn.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Chang
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. zhangshu@caas.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Agriculture and Food Sciences, University of Queensland, Gatton, QLD, 4343, Australia. cena_wang@hotmail.com.
authors.forename: L X N
authors.initials: LX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. liuguohua@caas.cn.
authors.forename: Guohua
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. binyao@caas.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D004734:Energy Metabolism; D063990:Gene Ontology; D008099:Liver / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D019936:Probiotics; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D020543:Proteome
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