title: [Measurement of Nicotine, Tobacco-Specific Nitrosamines, and Additives in the Filler and Gas from Japanese Brand Snuff].
abstract: OBJECTIVE
  The World Health Organization (WHO) Framework Convention on Tobacco Control (FCTC) reported that understanding the use and impact of smokeless tobacco (SLT) products is complicated by product diversity. Many different SLT products with different characteristics are used worldwide. ZERO STYLE STIX(TM) (sold by Japan Tobacco Inc.) is a brand of snuff, a type of smokeless tobacco. Our objective was to determine the constituents of the gas from SLT and analyze the ingredients in tobacco fillers.
  
  
  METHODS
  ZERO STYLE STIX smokeless tobacco was released in the Japanese market in 2010. Nicotine, menthol, and tobacco-specific nitrosamines in the smokeless tobacco fillers were determined by gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-tandem mass spectrometry (LC/MS/MS). The gaseous compounds were collected by a smoking machine using two smoking protocols, i.e., the ISO and Health Canada Intense methods. Nicotine and menthol in the gas were determined by GC/MS.
  
  
  RESULTS
  Nicotine, menthol, and the total tobacco-specific nitrosamines were detected in the tobacco fillers. The level of menthol in the snuff was more than ten times that of nicotine. The determined levels of the two components of the gas from the snuff were higher when using the Health Canada Intense protocol than when using the ISO protocol. In addition, flavors other than menthol were emitted from the smokeless tobacco.
  
  
  CONCLUSIONS
  The new type of snuff introduced in the Japanese market in 2010 contained added flavors, and was attractive smokeless tobacco. Flavors in tobacco products need to regulate on the basis of FCTC 9 and 10 in JAPAN.
authors.affiliation: Department of Environmental Health, National Institute of Public Health.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Inaba
authors.forename: Rika
authors.initials: R
authors.lastname: Utsugi
authors.forename: Tadamichi
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ohta
authors.forename: Naoki
authors.initials: N
authors.lastname: Kunugita
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D009538:Nicotine / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014030:Tobacco, Smokeless
chemical_list: D009602:Nitrosamines; D009538:Nicotine