title: Circulating Neutrophil MicroRNAs as Biomarkers for the Detection of Lung Cancer.
abstract: Peripheral neutrophils are the predominant circulating leukocytes and an important component of innate and adaptive immune systems, which is a primary defense against cancer. MicroRNAs (miRNAs) can modulate neutrophil functions and play important roles in cancer pathogenesis by regulating neutrophil gene expression. To investigate if assessment of differential miRNA levels of peripheral neutrophils has the potential for diagnosis of non-small-cell lung cancer (NSCLC), we examine neutrophils of 15 patients with stage I NSCLC and 15 smokers without cancer. We identify five neutrophil miRNAs that have an abnormal level in patients with NSCLC versus smokers without cancer. In a training set of 82 patients with lung cancer and 73 controls, a set of two genes (miRs-26a-2-3p and 574-3p) are developed, producing 77.8% sensitivity and 78.1% specificity for NSCLC detection. Furthermore, in a testing set of 60 patients with lung cancer and 58 smokers, the performance of analyzing the two miRNAs for lung cancer detection is confirmed. This study for the first time shows that a neutrophil miRNA profile may serve as a new category of circulating biomarkers for the detection of NSCLC.
authors.affiliation: Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.; Department of Clinical Biochemistry, Jiangsu University School of Medicine, Zhenjiang, Jiangsu Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.; College of Chinese Traditional Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Chhavi
authors.initials: C
authors.lastname: Gupta
authors.affiliation: Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
keywords: biomarkers; diagnosis; lung cancer; microRNA; peripheral neutrophils
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