title: TD-GC-MS Investigation of the VOCs Released from Blood Plasma of Dogs with Cancer.
abstract: An analytical TD-GC-MS method was developed and used for the assessment of volatile organic compounds (VOCs) released from the blood plasma of dogs with/without cancer. VOCs released from 40 samples of diseased blood and 10 control samples were compared in order to examine the difference between both sample groups that were showing qualitatively similar results independent from the disease's presence. However, mild disturbances in the spectra of dogs with cancer in comparison with the control group were observed, and six peaks (tentatively identified by comparison with mass spectral library as hexanal, octanal, toluene, 2-butanone, 1-octen-3-ol and pyrrole) revealed statistically significant differences between both sample groups, thereby suggesting that these compounds are potential biomarkers that can be used for cancer diagnosis based on the blood plasma TD-GC-MS analysis. Statistical comparison with the application of principal component analysis (PCA) provided accurate discrimination between the cancer and control groups, thus demonstrating stronger biochemical perturbations in blood plasma when cancer is present.
authors.affiliation: Graduate School of Environmental Engineering, the University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan. romanselyanchyn@gmail.com.
authors.forename: Roman
authors.initials: R
authors.lastname: Selyanchyn
authors.affiliation: Graduate School of Environmental Engineering, the University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan. s1maa007@eng.kitakyu-u.ac.jp.
authors.forename: Takuma
authors.initials: T
authors.lastname: Nozoe
authors.affiliation: Shinkou Seiki Co. Ltd., 1-18-3, Maidashi, Higashi-ku, Fukuoka 812-8581, Japan. matsui@shinkouseiki.co.jp.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, 582, Midorimachi, Bunkyodai, Ebetsu 069-8501, Japan. kado@rakuno.ac.jp.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kadosawa
authors.affiliation: Graduate School of Environmental Engineering, the University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan. leesw@kitakyu-u.ac.jp.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Lee
keywords: TD-GC-MS; cancer markers; dog blood plasma; volatile organic compounds
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