title: [Application of fluorescent in situ hybridization in primary diagnosis of aplastic anemia by morphology].
abstract: OBJECTIVE
  To investigate the clinical application of fluorescent in situ hybridization (FISH) for the differential diagnosis of myelodysplastic syndromes (MDS) and aplastic anemia (AA).
  
  
  METHODS
  A FISH kit capable of detecting the chromosomal abnormalities related to MDS was used to analyze 94 patients who were suspected to have AA by bone marrow morphology.
  
  
  RESULTS
  Cytogenetic abnormalities were detected in 11 of the 94 patients, which included trisomy 8 (5 cases), 20q- (1 case) and -Y (1 case). There were 4 cases related to MDS, which included 3 cases of 5q-, in which 1 case carry 20q- at the same time, and 7q- (1 case). No significant difference was found between the MDS and AA groups in terms of age, sex or routine blood examination including absolute neutrophil count, hemoglobin content and platelet count.
  
  
  CONCLUSIONS
  FISH can detect certain cytogenetic abnormalities related to MDS in patients morphologically diagnosed as AA.
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, China. huanghuif@126.com.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Lin
authors.forename: Wanzi
authors.initials: W
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Jiadi
authors.initials: J
authors.lastname: Chen
authors.forename: Huifang
authors.initials: H
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000175:diagnosis* / Q000235:genetics*; D001854:Bone Marrow Cells / Q000166:cytology*; D002648:Child; D002869:Chromosome Aberrations; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D014314:Trisomy / Q000235:genetics