title: Transcriptomic Sequencing Reveals a Set of Unique Genes Activated by Butyrate-Induced Histone Modification.
abstract: Butyrate is a nutritional element with strong epigenetic regulatory activity as a histone deacetylase inhibitor. Based on the analysis of differentially expressed genes in the bovine epithelial cells using RNA sequencing technology, a set of unique genes that are activated only after butyrate treatment were revealed. A complementary bioinformatics analysis of the functional category, pathway, and integrated network, using Ingenuity Pathways Analysis, indicated that these genes activated by butyrate treatment are related to major cellular functions, including cell morphological changes, cell cycle arrest, and apoptosis. Our results offered insight into the butyrate-induced transcriptomic changes and will accelerate our discerning of the molecular fundamentals of epigenomic regulation.
authors.affiliation: United States Department of Agriculture, Agriculture Research Service, Animal Genomics and Improvement Laboratory, Beltsville, MD, USA.
authors.forename: Cong-Jun
authors.initials: CJ
authors.lastname: Li
authors.affiliation: United States Department of Agriculture, Agriculture Research Service, Animal Genomics and Improvement Laboratory, Beltsville, MD, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Li
authors.affiliation: United States Department of Agriculture, Agriculture Research Service, Animal Genomics and Improvement Laboratory, Beltsville, MD, USA.
authors.forename: Ransom L
authors.initials: RL
authors.lastname: Baldwin
authors.affiliation: United States Department of Agriculture, Agriculture Research Service, Animal Biosciences and Biotechnology Laboratory, Beltsville, MD, USA.
authors.forename: Le Ann
authors.initials: le A
authors.lastname: Blomberg
authors.affiliation: Informatics Group, J. Craig Venter Institute, La Jolla, CA. USA.
authors.forename: Sitao
authors.initials: S
authors.lastname: Wu
authors.affiliation: Informatics Group, J. Craig Venter Institute, La Jolla, CA. USA.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Li
keywords: bovine cells; butyrate; gene regulation; histone modification; transcriptomic
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