title: Expression of programmed death ligand-1 on tumor cells varies pre and post chemotherapy in non-small cell lung cancer.
abstract: The effects of treatments to programmed death ligand-1 (PD-L1) expression is unknown. The aim of this study was to investigate the impact of neoadjuvant chemotherapy (NACT) on PD-L1 expression in non-small cell lung cancer (NSCLC) patients. PD-L1 expression was detected by immunohistochemistry (IHC) method in 32 paired tumor specimens pre and post-NACT. The positivity of PD-L1 on tumor cells (TCs) changed from 75% to 37.5% after NACT (p = 0.003). Cases with IHC score of 1, 2, 3 all underwent apparent decrease (p = 0.007). However, no significant changes were observed on tumour-infiltrating immune cells (ICs) (p = 0.337). Subgroup and semiquantitative analyses all presented similar results. Moreover, patients with response to NACT presented significantly reduced PD-L1 expression on TCs (p = 0.004). Although it was not confirmed by the Cox proportional hazard regression model, there was an apparent difference in disease-free-survival (DFS) between negative-to-positive switch of PD-L1 status and the contrary group (median DFS: 9.6 versus 25.9, p = 0.005). Our data revealed that antecedent chemotherapy for NSCLC may results in inconsistency of PD-L1 expression. PD-L1 expression is suggested to be monitored around treatment and on serial samples, at least, on the latest tumor specimen.
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sheng
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Fang
authors.affiliation: Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Medical Oncology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhan
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.lastname: Yanhuang
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.|Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.|Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D060890:B7-H1 Antigen; D014408:Biomarkers, Tumor
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