title: Insight into the possible mechanism of the summer diapause of Delia antiqua (Diptera: Anthomyiidae) through digital gene expression analysis.
abstract: The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic resources. To gain insight into the possible mechanism of summer diapause (SD), high-throughput RNA-Seq data were generated from non-diapause (ND) and SD (initial, maintenance and quiescence phase) pupae. Three pair-wise comparisons were performed and identified, 1380, 1471 and 435, and were significantly regulated transcripts. Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation, cell cycle, carbon hydrate and lipid metabolism, innate immune and stress responses, various signalling transductions, ubiquitin-dependent proteosome, and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD. Global characterization of transcriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD. Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Hao
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China.
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Si
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China.
authors.forename: Dan-Ying
authors.initials: DY
authors.lastname: Fu
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China.
authors.forename: Zheng-Bo
authors.initials: ZB
authors.lastname: He
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
mesh_terms: D000818:Animals; D064666:Diapause, Insect; D004175:Diptera / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism; D011679:Pupa / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D012621:Seasons
chemical_list: D007605:Juvenile Hormones
keywords: Delia antiqua; RNA-Seq; digital gene expression; possible mechanism; qPCR; summer diapause