title: 2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis.
abstract: We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was improved by adding purified beta-glucosidase from Thermobifida fusca YX (Tfu_0937) in the fermentation. Encouraged by these findings, we found that hydrolysis of cellobiose to glucose was an important reaction of 23BD biosynthesis in B. subtilis using cellobiose. Hence, we created efficient 23BD production from cellobiose using exogenous Tfu_0937-expressing B. subtilis. Using the engineered strain, 21.2 g L(-1) of 23BD was produced after 72 h of cultivation. The productivity and yield were 0.294 g L(-1) h(-1) and 0.35 g 23BD/g cellobiose, respectively. We successfully demonstrated efficient 23BD production from cellobiose by using BGL-expressing B. subtilis.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada, Kobe, 657-8501, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tanimura
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada, Kobe, 657-8501, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Takashima
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1, Rokkodai-cho, Nada, Kobe, 657-8501, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada, Kobe, 657-8501, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada, Kobe, 657-8501, Japan. akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002072:Butylene Glycols / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; D002475:Cellobiose; C026978:2,3-butylene glycol; D001617:beta-Glucosidase; D005947:Glucose
keywords: 2,3-Butanediol; B. subtilis; Beta-glucosidase; Cellobiose