title: [Chromosome examination of missed abortion patients].
abstract: OBJECTIVE
  To investigate the relationship between the missed abortion and chromosome abnormality and guide the healthy birth.
  
  
  METHODS
  From June 2014 to April 2015 in Tianjin central hospital of gynecology and obstetrics, we examined venous blood from 90 missed abortion couples for chromosome karyotype by lymphocyte culture method and we also examined their chromosome karyotype of abortion villus samples by high-throughput sequencing technologies.
  
  
  RESULTS
  Out of the 90 couples' blood chromosome examinations, 7 were abnormal, and the abnormal rate was 3.89%, including 3 cases reciprocal translocation, 2 cases robertsonian translocation and 2 cases inversion. Abortion villus samples from the same population were also checked, of which 85 cases succeeded, with the success rate of 94.4%. Among them, villi chromosome abnormalities were found in 50 cases, including 39 cases with abnormal chromosome numbers, 11 cases with abnormal chromosome structure, and the total abnormal rate was 58.8%. In addition, the villi chromosome abnormality rate of patients with recurrent missed abortion (≥2 times) and first missed abortion were 61.7% and 55.2%, respectively, and the difference was not significant (P>0.05). The villi chromosome abnormality rate of pregnant women with age≥35 years old was 71.1%, while the pregnant women with aged <35 years old was 45% (P<0.05).
  
  
  CONCLUSIONS
  Chromosome abnormality is an important cause of missed abortion; villi chromosome abnormality rate has nothing to do with the number of missed abortion; pregnant woman with age≥35 years old is risk factor of the villi chromosome abnormality.
authors.affiliation: Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, China.
authors.forename: Haomei
authors.initials: H
authors.lastname: Hu
authors.affiliation: Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, China; Email: huayangnv@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, China.
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000030:Abortion, Missed; D000328:Adult; D025063:Chromosome Disorders; D007446:Chromosome Inversion; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D007621:Karyotyping; D011247:Pregnancy; D012307:Risk Factors; D014178:Translocation, Genetic