title: Porous biodegradable EW62 medical implants resist tumor cell growth.
abstract: Magnesium alloys have been widely investigated for biodegradable medical applications. However, the shielding of harmful cells (eg. bacteria or tumorous cells) from immune surveillance may be compounded by the increased porosity of biodegradable materials. We previously demonstrated the improved corrosion resistance and mechanical properties of a novel EW62 (Mg-6%Nd-2%Y-0.5%Zr)) magnesium alloy by rapid solidification followed by extrusion (RS) compared to its conventional counterpart (CC). The present in vitro study evaluated the influence of rapid solidification on cytotoxicity to murine osteosarcoma cells. We found that CC and RS corrosion extracts significantly reduced cell viability over a 24-h exposure period. Cell density was reduced over 48 h following direct contact on both CC and RS surfaces, but was further reduced on the CC surface. The direct presence of cells accelerated corrosion for both materials. The corroded RS material exhibited superior mechanical properties relative to the CC material. The data show that the improved corrosion resistance of the rapidly solidified EW62 alloy (RS) resulted in a relatively reduced cytotoxic effect on tumorous cells. Hence, the tested alloy in the form of a rapidly solidified substance may introduce a good balance between its biodegradation characteristics and cytotoxic effect towards cancerous and normal cells.
authors.affiliation: Department of Materials Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors.forename: O
authors.initials: O
authors.lastname: Hakimi
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Ventura
authors.affiliation: Biomedical Engineering Department, Michigan Technological University, Houghton, MI 49931, USA. Electronic address: jgoldman@mtu.edu.
authors.forename: J
authors.initials: J
authors.lastname: Goldman
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Vago
authors.affiliation: Department of Materials Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Aghion
mesh_terms: D020341:Absorbable Implants; D000497:Alloys / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003343:Corrosion; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D016062:Porosity
chemical_list: D000497:Alloys; D008274:Magnesium
keywords: Corrosion; Cytotoxicity; Magnesium alloy; Tumor cells