title: Reorganization of Basolateral Amygdala-Subiculum Circuitry in Mouse Epilepsy Model.
abstract: In this study, we investigated the reorganized basolateral amygdala (BLA)-subiculum pathway in a status epilepticus (SE) mouse model with epileptic episodes induced by pilocarpine. We have previously observed a dramatic loss of neurons in the CA1-3 fields of the hippocampus in epileptic mice. Herein, we observed a 43-57% reduction in the number of neurons in the BLA of epileptic mice. However, injection of an anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHA-L) into the BLA indicated 25.63% increase in the number of PHA-L-immunopositive terminal-like structures in the ventral subiculum (v-Sub) of epileptic mice as compared to control mice. These data suggest that the projections from the basal nucleus at BLA to the vSub in epileptic mice are resistant to epilepsy-induced damage. Consequently, these epileptic mice exhibit partially impairment but not total loss of context-dependent fear memory. Epileptic mice also show increased c-Fos expression in the BLA and vSub when subjected to contextual memory test, suggesting the participation of these two brain areas in foot shock-dependent fear conditioning. These results indicate the presence of functional neural connections between the BLA-vSub regions that participate in learning and memory in epileptic mice.
authors.affiliation: Program in Neuroscience and Behavioral Disorder, Duke-NUS Graduate Medical School Singapore, Singapore.
authors.forename: Dong Liang
authors.initials: DL
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, China.
authors.forename: Jian Qiang
authors.initials: JQ
authors.lastname: Qu
authors.affiliation: Program in Neuroscience and Behavioral Disorder, Duke-NUS Graduate Medical SchoolSingapore, Singapore; Department of Physiology, Yong Loo Lin School of Medicine, National University of SingaporeSingapore, Singapore; KK Research Center, KK Women's and Children's HospitalSingapore, Singapore.
authors.forename: Eyleen L K
authors.initials: EL
authors.lastname: Goh
authors.affiliation: Temasek Laboratories, National University of SingaporeSingapore, Singapore; Singapore Nuclear Research and Safety Initiative, National University of SingaporeSingapore, Singapore.
authors.forename: Feng Ru
authors.initials: FR
authors.lastname: Tang
keywords: basolateral amygdala; c-Fos; contextual fear memory test; epilepsy; mouse; pilocarpine; reorganization; subiculum
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