title: Effect of collagen sponge and fibrin glue on bone repair.
abstract: The ability of hemostatic agents to promote bone repair has been investigated using in vitro and in vivo models but, up to now, the results are inconclusive. Objective In this context, the aim of this study was to compare the potential of bone repair of collagen sponge with fibrin glue in a rat calvarial defect model.
  
  
  METHODS
  Defects of 5 mm in diameter were created in rat calvariae and treated with either collagen sponge or fibrin glue; untreated defects were used as control. At 4 and 8 weeks, histological analysis and micro-CT-based histomorphometry were carried out and data were compared by two-way ANOVA followed by Student-Newman-Keuls test when appropriated (p≤0.05).
  
  
  RESULTS
  Three-dimensional reconstructions showed increased bone formation in defects treated with either collagen sponge or fibrin glue compared with untreated defects, which was confirmed by the histological analysis. Morphometric parameters indicated the progression of bone formation from 4 to 8 weeks. Additionally, fibrin glue displayed slightly higher bone formation rate when compared with collagen sponge.
  
  
  CONCLUSIONS
  Our results have shown the benefits of using collagen sponge and fibrin glue to promote new bone formation in rat calvarial bone defects, the latter being discreetly more advantageous.
authors.affiliation: Laboratório de Cultura de Células, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Thiago de Santana
authors.initials: Tde S
authors.lastname: Santos
authors.affiliation: Laboratório de Cultura de Células, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Rodrigo Paolo Flores
authors.initials: RP
authors.lastname: Abuna
authors.affiliation: Laboratório de Cultura de Células, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Adriana Luisa Gonçalves de
authors.initials: AL
authors.lastname: Almeida
authors.affiliation: Laboratório de Cultura de Células, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Marcio Mateus
authors.initials: MM
authors.lastname: Beloti
authors.affiliation: Laboratório de Cultura de Células, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Adalberto Luiz
authors.initials: AL
authors.lastname: Rosa
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D017102:Fracture Healing / Q000187:drug effects; D006490:Hemostatics / Q000494:pharmacology*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012886:Skull / Q000187:drug effects / Q000293:injuries; D013552:Swine; D013997:Time Factors; D016896:Treatment Outcome; D055114:X-Ray Microtomography
chemical_list: D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D003094:Collagen
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