title: Homocysteine, endothelin-1 and nitric oxide in patients with hypertensive disorders complicating pregnancy.
abstract: OBJECTIVE
  To investigate the change of level of serum homocysteine (Hcy), endothelin-1 (ET-1) and nitric oxide (NO) and clinical significance in patients with HDCP.
  
  
  METHODS
  Two hundred and thirty nine patients with HDCP (137 patients with mild preeclampsia, 102 patients with severe preeclampsia) who were hospitalized between June 2012 and June 2015 and 200 normal pregnancy women in outpatient department were enrolled in our study were divided into HDCP group and control group. Serum Hcy concentration was measured by enzymatic cycling assay. ET-1 concentration was measured by enzyme linked immunosorbent assay. And no concentration was measured by nitrate reductase assay.
  
  
  RESULTS
  Serum Hcy and ET-1 in HDCP group were significantly higher as compared to control group (P<0.05). Level of serum NO in HDCP group was significantly lower than in the control group (P<0.05). Level of serum Hcy and ET-1 in mild and severe preeclampsia group were significantly higher as compared to control group, respectively (P<0.05). Level of serum NO in mild and severe preeclampsia group were significantly lower than in the control group' respectively (P<0.05). Level of serum Hcy and ET-1 in severe preeclampsia group were significantly higher as compared to mild preclampsia group (P<0.05). Level of serum NO in severe preeclampsia group were significantly lower than in mild preeclampsia group (P<0.05). Spearman rank correlation analysis showed that level of serum Hcy and ET-1 was positively correlated with severity of diseases (r=0.689, 0.718, P<0.05). Level of serum NO was negatively correlated with severity of diseases (r=-0.702, P<0.05).
  
  
  CONCLUSIONS
  Serum Hcy, ET-1 and NO were associated with pathogenesis of HDCP. Comprehensively measurement of them could effectively evaluate the incidence and progress of HDCP.
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, PR China.
authors.forename: Yachang
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, PR China.
authors.forename: Mujun
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, PR China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Wang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D016022:Case-Control Studies; D019332:Endothelin-1 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D009569:Nitric Oxide / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology; D011237:Predictive Value of Tests; D011247:Pregnancy; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D019332:Endothelin-1; D006710:Homocysteine; D009569:Nitric Oxide
keywords: Gestational hypertension; endothelin-1; homocysteine; nitric oxide
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