title: Hymenobacter rubidus sp. nov., bacterium isolated from a soil.
abstract: Strain DG7B(T) was isolated from a soil sample collected in Seoul, Republic of Korea and was observed to be a gram-negative, short-rod shaped and non-motile bacterium. Its 16S rRNA gene sequence is closely related to those of Hymenobacter terrae DG7A(T) (97.8 % similarity), H. soli PB17(T) (97.5 %), H. glaciei VUG-A130(T) (96.4 %), H. saemangeumensis GSR0100(T) (95.7 %), H. ruber PB156(T) (95.3 %), and H. antarcticus VUG-A42aa(T) (95.3 %). The low levels of DNA-DNA relatedness (<50.3 %) with the above species identified strain DG7B(T) as a novel species in the genus Hymenobacter. The genomic DNA G+C content was determined to be 54.9 %. Growth of strain DG7B(T) was observed at 12-30 °C (optimum at 25 °C) and pH 6.0-11.0 (optimum at pH 7). The cells tolerate <0.5 % NaCl. A UV-visible scan of an ethanol extract of the whole cell pigment showed absorbance peaks at 264.5, 320.0, and 481.5 nm, so the pigment type was determined to be 2'-hydroxyflexixanthin. Chemotaxonomic data showed that strain DG7B(T) possesses menaquinone-7 as the predominant isoprenoid quinone, sym-homospermidine as the major polyamine, phosphatidylethanolamine as the predominant polar lipid and iso-C15:0, anteiso-C15:0 and summed feature 3 (C16:1 ω7c/C16:1 ω7c) as the major fatty acids. Strain DG7B(T) showed low-level resistance to ultraviolet C. Based on the polyphasic analysis, it is concluded that strain DG7B(T) (=KCTC 32553(T) = KEMB 9004-166(T) = JCM 30008(T)) should be classified as the type strain of a novel Hymenobacter species, for which the name Hymenobacter rubidus sp. nov. is proposed.
authors.affiliation: Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea.
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Joo
authors.affiliation: Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea.
authors.forename: Eun Bit
authors.initials: EB
authors.lastname: Kim
authors.affiliation: Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea.
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Jeon
authors.affiliation: Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.affiliation: Division of Environmntal & Life Science, Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul, 139-774, Republic of Korea. biotech@swu.ac.kr.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002693:Chlamydiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects; D016680:Genome, Bacterial; D050296:Microbial Viability / Q000528:radiation effects; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011836:Radiation Tolerance / Q000528:radiation effects; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014466:Ultraviolet Rays
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Cytophagaceae; Hymenobacter; Taxonomic