title: Spectral karyotyping: an unique technique for the detection of complex genomic rearrangements in leukemia.
abstract: Spectral karyotyping (SKY) is a novel cytogenetic technique, has been developed to unambiguously display and identify all 24 humans chromosomes at one time without a priori knowledge of any abnormalities involved. SKY can discern the aberrations that can't be detected very well by conventional banding technique and Fluorescent in situ hybridization (FISH). So SKY is hyper accurate, hypersensitive, and hyper intuitionist. We will review the elements and application of SKY in leukemia.
authors.affiliation: 1 Department of Hematology, 2 Department of Hematology, General Hospital of PLA, Beijing 100853, China ; 3 Western Sydney Genomic Diagnostics, Western Sydney Genetics Program, The Children's Hospital at Westmead, NSW, Australia.
authors.forename: Bo
authors.initials: B
authors.lastname: Guo
authors.affiliation: 1 Department of Hematology, 2 Department of Hematology, General Hospital of PLA, Beijing 100853, China ; 3 Western Sydney Genomic Diagnostics, Western Sydney Genetics Program, The Children's Hospital at Westmead, NSW, Australia.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Han
authors.affiliation: 1 Department of Hematology, 2 Department of Hematology, General Hospital of PLA, Beijing 100853, China ; 3 Western Sydney Genomic Diagnostics, Western Sydney Genetics Program, The Children's Hospital at Westmead, NSW, Australia.
authors.forename: Zhanhe
authors.initials: Z
authors.lastname: Wu
authors.affiliation: 1 Department of Hematology, 2 Department of Hematology, General Hospital of PLA, Beijing 100853, China ; 3 Western Sydney Genomic Diagnostics, Western Sydney Genetics Program, The Children's Hospital at Westmead, NSW, Australia.
authors.forename: Wanming
authors.initials: W
authors.lastname: Da
authors.affiliation: 1 Department of Hematology, 2 Department of Hematology, General Hospital of PLA, Beijing 100853, China ; 3 Western Sydney Genomic Diagnostics, Western Sydney Genetics Program, The Children's Hospital at Westmead, NSW, Australia.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhu
keywords: Spectral karyotyping (SKY); chromosome analysis; leukemia
references.citation: Genes Chromosomes Cancer. 1999 Dec;26(4):336-45
references.pmid: 10534769
references.citation: Genet Test. 2000;4(1):9-14
references.pmid: 10794355
references.citation: Clin Genet. 2001 Feb;59(2):65-73
references.pmid: 11260203
references.citation: Br J Haematol. 2001 May;113(2):305-17
references.pmid: 11380393
references.citation: Genes Chromosomes Cancer. 2002 Jun;34(2):137-53
references.pmid: 11979548
references.citation: Acta Haematol. 2002;107(4):224-9
references.pmid: 12053151
references.citation: Leukemia. 2002 Nov;16(11):2222-7
references.pmid: 12399965
references.citation: Genes Chromosomes Cancer. 2003 Nov;38(3):249-52
references.pmid: 14506699
references.citation: Leuk Res. 2004 Mar;28(3):275-84
references.pmid: 14687623
references.citation: Diagn Mol Pathol. 2004 Sep;13(3):190-5
references.pmid: 15322432
references.citation: Cancer Genet Cytogenet. 2005 Jan 1;156(1):59-61
references.pmid: 15588857
references.citation: Semin Oncol. 2008 Aug;35(4):365-77
references.pmid: 18692687
references.citation: Cancer Genet Cytogenet. 2009 Apr 1;190(1):26-32
references.pmid: 19264230
references.citation: Cancer Genet Cytogenet. 2010 Jun;199(2):134-8
references.pmid: 20471517
references.citation: Cancer Genet Cytogenet. 2010 Sep;201(2):111-5
references.pmid: 20682395
references.citation: Pathology. 2011 Feb;43(2):180-2
references.pmid: 21233687
references.citation: Hum Pathol. 2013 Oct;44(10):2365-9
references.pmid: 23759653
references.citation: EXCLI J. 2012 Mar 01;11:89-92
references.pmid: 27298606
references.citation: Nat Genet. 1996 Apr;12(4):368-75
references.pmid: 8630489
references.citation: Science. 1996 Jul 26;273(5274):494-7
references.pmid: 8662537
references.citation: Nat Genet. 1997 Apr;15(4):406-10
references.pmid: 9090389
references.citation: Hum Genet. 1997 Dec;101(3):255-62
references.pmid: 9439652