title: Low-intensity treadmill exercise promotes rat dorsal wound healing.
abstract: In order to investigate the promoting effect of low-intensity treadmill exercise on rat dorsal wound healing and the mechanism, 20 Sprague-Dawley rats were randomly divided into two groups: exercise group (Ex) and non-exercise group (non-ex). The rats in Ex group were given treadmill exercise for one month, and those in non-ex group raised on the same conditions without treadmill exercise. Both groups received dorsal wound operation with free access to food and water. By two-week continuous observation and recording of the wound area, the healing rate was analyzed. The blood sample was collected at day 14 post-operation via cardiac puncture for determination of the number of endothelial progenitor cells (EPCs) by flow cytometry, and the concentrations of relevant cytokines such as basic fibroblast growth factor (bFGF), endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) were measured by ELISA. The skin tissue around the wound was dissected to observe the vascular density under the microscope after HE staining, to detect the mRNA level of VEGFR2 and angiopoietin-1 (Ang-1) receptor using RT-qPCR, and protein expression of a-smooth muscle actin (αSMA) and type III collagen (ColIII) using Western blotting. It was found that the wound area in Ex group was smaller at the same time point than in non-ex group. The number of circulating EPCs was greater and the concentrations of vasoactive factors such as VEGF, eNOS and bFGF were higher in Ex group than in non-ex group. HE staining displayed a higher vessel density in Ex group than in non-ex group. Moreover, the mRNA expression of VEGFR2 and Ang-1 detected in the wound tissue in Ex group was higher than in non-ex group. Meanwhile, the protein expression of αSMA and ColIII was more abundant in Ex group than in non-ex group. Conclusively, the above results demonstrate Ex rats had a higher wound healing rate, suggesting low-intensity treadmill exercise accelerates wound healing. The present work may provide some hint for future study of treating refractory wound.
authors.affiliation: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. liuguohui@medmail.com.cn.
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. shuhua_yang@yeah.net.
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Fourth Military Medical College Affiliated Tangdu Hospital, Xi'an, 710032, China.
authors.forename: Bo-Bin
authors.initials: BB
authors.lastname: Mi
authors.affiliation: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Shu-Nan
authors.initials: SN
authors.lastname: Ye
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D024061:Collagen Type III / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D066026:Endothelial Progenitor Cells / Q000166:cytology; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000097:blood; D005082:Physical Exertion; D012333:RNA, Messenger / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D042810:Receptor, TIE-1 / Q000378:metabolism; D012420:Running; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000097:blood; D014945:Wound Healing
chemical_list: D000199:Actins; D024061:Collagen Type III; D016207:Cytokines; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger; D042810:Receptor, TIE-1; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2; C498862:Acta2 protein, rat; C000602029:Kdr protein, rat
keywords: angiopoietin; endothelial progenitor cells; treadmill exercise; vascular endothelial growth factor; wound healing
references.citation: Biomaterials. 2011 Oct;32(29):7096-105
references.pmid: 21741704
references.citation: Arthritis Res. 2002;4(3):201-8
references.pmid: 12010571
references.citation: Life Sci. 2009 Feb 13;84(7-8):240-9
references.pmid: 19135459
references.citation: J Cell Mol Med. 2013 Mar;17(3):386-97
references.pmid: 23414097
references.citation: Acta Odontol Scand. 2008 Feb;66(1):31-7
references.pmid: 18320416
references.citation: Adv Skin Wound Care. 2013 Nov;26(11):512-24; quiz 225-6
references.pmid: 24149666
references.citation: Atherosclerosis. 2005 Aug;181(2):305-10
references.pmid: 16039284
references.citation: Cardiovasc Diabetol. 2013 Feb 19;12:34
references.pmid: 23421785
references.citation: Tissue Eng Part A. 2009 Jun;15(6):1269-80
references.pmid: 18939935
references.citation: Stem Cells. 2005 Nov-Dec;23(10):1571-8
references.pmid: 16081667
references.citation: Gene. 2013 Aug 10;525(2):220-8
references.pmid: 23566831
references.citation: Am J Pathol. 2005 Nov;167(5):1257-66
references.pmid: 16251410
references.citation: J Am Coll Cardiol. 2004 Jun 16;43(12):2314-8
references.pmid: 15193699
references.citation: Ann Surg. 2011 Dec;254(6):1066-74
references.pmid: 21832942
references.citation: Cell Biol Int. 2012 Oct 1;36(10):945-50
references.pmid: 22731705
references.citation: Med Sci Sports Exerc. 2012 Oct;44(10):1846-54
references.pmid: 22543735
references.citation: Am J Physiol Regul Integr Comp Physiol. 2008 Jan;294(1):R179-84
references.pmid: 18003791
references.citation: J Sports Med Phys Fitness. 1987 Sep;27(3):380-4
references.pmid: 3123800
references.citation: Cytokine. 2011 Jul;55(1):110-5
references.pmid: 21524919
references.citation: Bone. 2010 Jan;46(1):32-40
references.pmid: 19747991
references.citation: Acta Biomater. 2013 Aug;9(8):7814-21
references.pmid: 23603001