title: Subgroup J avian leukosis virus infection of chicken dendritic cells induces apoptosis via the aberrant expression of microRNAs.
abstract: Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus that causes immunosuppression and enhances susceptibility to secondary infection. The innate immune system is the first line of defense in preventing bacterial and viral infections, and dendritic cells (DCs) play important roles in innate immunity. Because bone marrow is an organ that is susceptible to ALV-J, the virus may influence the generation of bone marrow-derived DCs. In this study, DCs cultured in vitro were used to investigate the effects of ALV infection. The results revealed that ALV-J could infect these cells during the early stages of differentiation, and infection of DCs with ALV-J resulted in apoptosis. miRNA sequencing data of uninfected and infected DCs revealed 122 differentially expressed miRNAs, with 115 demonstrating upregulation after ALV-J infection and the other 7 showing significant downregulation. The miRNAs that exhibited the highest levels of upregulation may suppress nutrient processing and metabolic function. These results indicated that ALV-J infection of chicken DCs could induce apoptosis via aberrant microRNA expression. These results provide a solid foundation for the further study of epigenetic influences on ALV-J-induced immunosuppression.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China.|Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China.|Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou, People's Republic of China.
authors.forename: Manman
authors.initials: M
authors.lastname: Dai
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China.|Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China.|Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou, People's Republic of China.|South China Collaborative Innovation Center for Prevention and Control of Poultry Infectious Diseases and Safety of Poultry Products, Guangzhou, People's Republic of China.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China.|Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou, People's Republic of China.|South China Collaborative Innovation Center for Prevention and Control of Poultry Infectious Diseases and Safety of Poultry Products, Guangzhou, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001353:Avian Leukosis / Q000235:genetics* / Q000821:virology*; D001354:Avian Leukosis Virus / Q000502:physiology*; D002454:Cell Differentiation; D048430:Cell Shape; D002478:Cells, Cultured; D002645:Chickens / Q000821:virology*; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics
chemical_list: D035683:MicroRNAs
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