title: C/EBPα inhibits proliferation of breast cancer cells via a novel pathway of miR-134/CREB.
abstract: C/EBPα plays an important role in the modulation of cell proliferation, differentiation or apoptosis in various tissues. Most recently, reduced expression of C/EBPα and growth inhibitory effect was found in primary mammary carcinomas. However, the underlying mechanism is still not fully aware. Here, we firstly identified miR-134 as a target of C/EBPα in MCF7 breast cancer cell lines. C/EBPα overexpression promoted miR-134 expression, causing suppression of apoptosis- protective genes CREB and Bcl-2, and resulted in the proliferation inhibition of MCF7 cells. Moreover, anti-miR-134 rescued the proliferation inhibition of MCF7 cells and the suppression of anti-apoptotic genes CREB and Bcl-2 caused by C/EBPα overexpression. Collectively, C/EBPα inhibited cell growth in breast cancer cells via a novel pathway miR-134/CREB.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Shoujun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Integrated TCM & Western Medicine, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Yuanting
authors.initials: Y
authors.lastname: Gu
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: C000595707:BCL2 protein, human; D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C569273:MIRN134 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: C/EBPα; CREB; breast cancer cell; miR-134; proliferation
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