title: Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov., two tremellaceous yeast species from Brazil.
abstract: Two yeast species, Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov., in the family Rhynchogastremataceae of the Tremellales are proposed. The two species are related to six species of the genus Papiliotrema: Papiliotrema aureus, P. flavescens, P. terrestris, P. baii, P. ruineniae and P. wisconsinensis. The novel species are proposed on the basis of the sequence-based phylogenetic species concept with analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. A total of 16 strains of Papiliotrema leoncinii sp. nov. were obtained from freshwater and bromeliad leaves collected in Brazil. Papiliotrema leoncinii sp. nov. differs by 11, 12, 16, 14, 11 and 13 substitutions in the D1/D2 domain from the related species P. aureus, P. flavescens, P. terrestris, P. baii, P. ruineniae and P. wisconsinensis, respectively. Differences of 11 substitutions and 21 or more substitutions in ITS regions were found when the sequences of Papiliotrema leoncinii sp. nov. were compared with P. wisconsinensis and its closest relatives. The type strain of Papiliotrema leoncinii sp. nov. is UFMG-CM-Y374T (=CBS 13918T). Papiliotrema miconiae sp. nov. is represented by two strains isolated from a flower of Miconia sp. and a water sample in Brazil. Papiliotrema miconiae sp. nov. differs from the related species P. aureus and P. ruineniae by eight substitutions, from P. flavescens and P. terrestris by 11 substitutions, from P. baii by 10 substitutions and from P. wisconsinensis by 6 substitutions in the D1/D2 domain, and by 7 substitutions from P. wisconsinensis and more than 19 substitutions in the ITS region from its closest relatives. The type strain of Papiliotrema miconiae sp. nov. is CBS 8358T (ML 3666T=DBVPG-4492T). The MycoBank numbers for Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov. are MB 813594 and MB 814882, respectively.
authors.affiliation: Setor de Genética, Universidade Federal de Alagoas, ICBS, Maceió - AL, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Machado Pagani
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Luciana R
authors.initials: LR
authors.lastname: Brandão
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Ana Raquel O
authors.initials: ARO
authors.lastname: Santos
authors.affiliation: Setor de Genética, Universidade Federal de Alagoas, ICBS, Maceió - AL, Brazil.
authors.forename: Ciro R
authors.initials: CR
authors.lastname: Felix
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Jesus
authors.initials: J
authors.lastname: Pais Ramos
authors.affiliation: Setor de Genética, Universidade Federal de Alagoas, ICBS, Maceió - AL, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Broetto
authors.affiliation: Rosenstiel School of Marine and Atmospheric Science, University of Miami, Key Biscayne, FL, 33149, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Scorzetti
authors.affiliation: Rosenstiel School of Marine and Atmospheric Science, University of Miami, Key Biscayne, FL, 33149, USA.
authors.forename: Jack W
authors.initials: JW
authors.lastname: Fell
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG, 31270-901, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Augusto Rosa
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Valente
authors.affiliation: Setor de Genética, Universidade Federal de Alagoas, ICBS, Maceió - AL, Brazil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Fontes Landell
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D035264:Flowers / Q000382:microbiology; D020459:Genes, rRNA; D035322:Melastomataceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal