title: Glucosyl Rubusosides by Dextransucrases Improve the Quality of Taste and Sweetness.
abstract: Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59-66% as analyzed by TLC and HPLC. Evaluation of the sweetness of these glucosyl rubusosides showed that their quality of taste, in particular, was superior to that of rubusoside. These results indicate that transglucosylation at the 13-O-glucosyl moiety of rubusoside by different regioselective dextransucrases can be applicable for increasing its sweetness and quality of taste.
authors.affiliation: Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Ko
authors.affiliation: Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
authors.forename: Young Bae
authors.initials: YB
authors.lastname: Ryu
authors.affiliation: Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
authors.forename: Cha Young
authors.initials: CY
authors.lastname: Kim
authors.affiliation: Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
authors.forename: Joong Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology and BK21 Plus Program, Graduate School of Chonnam National University and Bioenergy Research Center, Gwangju 61186, Republic of Korea.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Nam
authors.affiliation: Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology and BK21 Plus Program, Graduate School of Chonnam National University and Bioenergy Research Center, Gwangju 61186, Republic of Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D055162:Biocatalysis; D045786:Diterpenes, Kaurane / Q000737:chemistry* / Q000378:metabolism; D005421:Flavoring Agents / Q000737:chemistry* / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry* / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007934:Leuconostoc / Q000201:enzymology*; D013649:Taste
chemical_list: D001426:Bacterial Proteins; D045786:Diterpenes, Kaurane; D005421:Flavoring Agents; D005960:Glucosides; D005964:Glucosyltransferases; C020025:dextransucrase; C056177:rubusoside
keywords: Acceptor reaction; Leuconostoc citreum; Leuconostoc lactis; dextransucrase; rubusoside