title: [The effect of noninvasive positive pressure ventilation on the expression of ubiquitin-proteasome of skeletal muscle in patients with AECOPD].
abstract: OBJECTIVE
  To investigate the effect of noninvasive positive pressure ventilation( NIPPy) on the gene and protein expression of biquitin-proteasome of skeletal muscle in patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).
  
  
  METHODS
  Seven patients with AECOPD by NIPPV were used as the study group, meanwhile, 6 patients with AECOPD who refused NIPPV was the control group. The blood gas analysis, heart rate (HR) and mean arterial pressure (MBp) were monitored before and 14 days after treatment. A skeletal muscle biopsy was performed after 14 days of therapy. The mRNA expression of ribosomal protein S21 (RPS21), Ubiquitin, Ubiquitin combined with enzyme E2 (E2), Ubiquitin ligase E3 (E3) in skeletal muscle cell were measured by RT-PCR. The protein expression of mitochondrial aconitase (AC02), protease C3 (C3), ribosomal protein SLC16 (SLC16) were detected by Western blot.
  
  
  RESULTS
  Forteen days after treatment, the patients in NIPPV group got much better improvement in PaCO2, PaO2 and HR than that of the patients.in the control group (P < 0.05). The gene expression of RPS21,Ubiquitin, E2 and E3 in skeletal muscle cell on patients with NIPPV were obviously lower than that of the control group (P < 0.05, P < 0.01). Compared with that of the control group, the protein expression of C3 and AC2 increased significantly in the NIPPV group (P < 0.01). The protein expression of SLC16 was significantly lowered in the treated group (P < 0.01).
  
  
  CONCLUSIONS
  NIPPV can ameliorate the proteasome pathway and energy metabolic disorders in patients with AECOPD.
authors.forename: Xi-yuan
authors.initials: XY
authors.lastname: Xu
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Yang
authors.forename: Tie-ying
authors.initials: TY
authors.lastname: Tian
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Ri-na
authors.initials: RN
authors.lastname: Wu
authors.forename: Hui-fang
authors.initials: HF
authors.lastname: Song
mesh_terms: D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism*; D011175:Positive-Pressure Respiration; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000628:therapy*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex