title: Effects on the ubiquitin proteasome system after closed soft-tissue trauma in rat skeletal muscle.
abstract: Previous studies have suggested that an increased catabolic stage of skeletal muscle in pathological situations is mainly a reflection of ubiquitin-proteasome system-controlled proteolysis. The proteolytic mechanisms that occur after local muscle trauma are poorly defined. We investigated the effects of closed soft-tissue trauma on ubiquitin-proteasome dependent protein breakdown in rats (n = 25). The enzymatic activities of the ubiquitination and proteasome reactions were both reduced (p < 0.05) immediately after contusion of the hind limb musculus extensor digitorum longus. The same effect was observed in extracts of lung tissue from the injured animals. Cellular levels of free and protein-conjugated ubiquitin were significantly elevated upon decreased proteolytic activity. Our data support an early-state anti-proteolytic role of the ubiquitin-proteasome pathway after local injury. This further implies that there is a yet-to-be elucidated complex regulatory mechanism of muscle regeneration that involves various proteolytic systems.
authors.affiliation: Trauma Research Laboratory, Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls-University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany. norbert.ponelies@medma.uni-heidelberg.de.
authors.forename: N
authors.initials: N
authors.lastname: Ponelies
authors.affiliation: Trauma Research Laboratory, Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls-University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Gosenca
authors.affiliation: Trauma Research Laboratory, Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls-University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Ising
authors.affiliation: Institute for Experimental Surgery, University of Rostock, Schillingallee, 18055, Rostock, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schoen
authors.affiliation: Trauma Research Laboratory, Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls-University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Ruppel
authors.affiliation: Institute for Experimental Surgery, University of Rostock, Schillingallee, 18055, Rostock, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Vollmar
authors.affiliation: Trauma Research Laboratory, Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls-University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Obertacke
keywords: Closed soft tissue trauma; Inflammatory myopathies; Muscular dystrophy; Skeletal muscle regeneration; Ubiquitin–proteasome system
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