title: The function of feline stimulator of interferon gene (STING) is evolutionarily conserved.
abstract: Stimulator of interferon gene (STING) mediates the induction of type I IFN responses. In this study, feline STING was cloned. Full-length STING contains 1134bp and encodes a 377 amino acid product that shares the highest similarity with bovine STING. STING is primarily expressed in the spleen, lungs and lymph nodes. An examination of its cellular localization indicated that STING is localized in the endoplasmic reticulum (ER) and contains two ER retention motifs, RPR and KKNF. Overexpressing STING induced the IFN response via the IRF3, NF-κB and AP-1 pathways. Moreover, the C-terminus of STING was required for the activation of IRF3 and AP-1. Knockdown of STING impaired the IFN-β response triggered by poly(dA:dT), poly(I:C) or SeV. Finally, STING activated the ISRE promoter and increased the expression of ISG15 and viperin. Collectively, our findings indicate that STING is involved in the regulation of the IFN-β pathway in felines.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
authors.forename: Xiaozhan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
authors.forename: Chunguo
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China; College of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China; College of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
authors.forename: Shaopo
authors.initials: S
authors.lastname: Zu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China. Electronic address: tj6049345@126.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China. Electronic address: qld@hvri.ac.cn.
authors.forename: Liandong
authors.initials: L
authors.lastname: Qu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, 510642, People's Republic of China. Electronic address: shoujunli@scau.edu.cn.
authors.forename: Shoujun
authors.initials: S
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000235:genetics*; D002417:Cattle; D004268:DNA-Binding Proteins / Q000235:genetics*; D019143:Evolution, Molecular; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D020539:Sequence Analysis, Protein; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D050838:Interferon Regulatory Factor-3; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D016899:Interferon-beta
keywords: Activation; Feline; STING; Type I IFN