title: A new kinetic-mechanistic approach to elucidate electrooxidation of doxorubicin hydrochloride in unprocessed human fluids using magnetic graphene based nanocomposite modified glassy carbon electrode.
abstract: A novel magnetic nanocomposite was synthesized in one step using polymerization of magnetic graph oxide grafted with chlorosulfonic acid (Fe3O4-GO-SO3H) in the presence of polystyrene. The prepared magnetic nanocomposite was characterized using transmission electron microscopy (TEM), dynamic differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), (Thermo-gravimetric/differential thermal analysis (DTA)), Fourier transform infrared (FTIR), and UV-Vis techniques. Magnetic nanocomposite was casted on the surface of the glassy carbon electrode (PS/Fe3O4-GO-SO3H/GCE) and used for the detection and determination of doxorubicin hydrochloride (DOX) in human biological fluids. The cyclic voltammograms (CVs) of the modified electrode in aqueous solution displayed a pair of well-defined, stable and irreversible reductive/oxidation redox systems. CV study indicated that the oxidation process is irreversible and adsorption controlled. In addition, CV results indicated that DOX is oxidized via two electrons and three protons which is an unusual approach for the oxidation of DOX. A sensitive and time-saving procedure was developed for the analysis of DOX in plasma, cerebrospinal fluid, and urine with detection limit of 4.9 nM, 14 nM and 4.3 nM, respectively.
authors.affiliation: Hematology-Oncology Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Soleymani
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Mhmmd_hasanzadeh@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hasanzadeh
authors.affiliation: Department of Nanochemistry, Nano Technology Center, and Faculty of Chemistry, Urmia University, Urmia, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Shadjou
authors.affiliation: Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Khoubnasab Jafari
authors.affiliation: Hematology-Oncology Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Jalil Vaez
authors.initials: JV
authors.lastname: Gharamaleki
authors.affiliation: Department of Organic Chemistry, Faculty of Chemistry, Tabriz University, Tabriz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Yadollahi
authors.affiliation: Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Jouyban
mesh_terms: D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism*; D004317:Doxorubicin / Q000032:analysis* / Q000493:pharmacokinetics*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D053761:Nanocomposites / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D006108:Graphite; D004317:Doxorubicin
keywords: Biological fluids; Doxorubicin; Electrochemical sensor; Graphene oxide; Magnetic nanocomposite; Polymerization