title: Application of Deuteporfin in the Metastatic Lymph Node Mapping of Pancreatic Cancer: An in vivo Study.
abstract: For most cancer patients, the presence of metastatic lymph nodes usually indicates regional recurrence and poor prognosis. Therefore, lymph node mapping is a requisite for disease staging, prognosis prediction and decision making in the treatment of cancer. Deuteporfin, a second-generation photosensitizer, has a maximum excitation wavelength that can reach the near infrared (NIR) region (650-700 nm). We aimed to take advantage of these aspects of deuteporfin and use it as a fluorescent probe for metastatic lymph node mapping in vivo using NIR fluorescent imaging. In our study, we further investigated whether a photosensitizer could be used as a tracer for metastatic lymph node mapping of pancreatic cancer based on previous reports. Compared to normal tissues, tumor tissues including primary tumors and metastatic lymph nodes had a higher uptake ability of deuteporfin (P < 0.05). Our research confirmed this targeting property of deuteporfin using in vivo fluorescent imaging. Consistent with observations from in vivo imaging experiments, frozen sections of metastatic lymph nodes intuitively displayed significantly higher and wider distributions of deuteporfin than normal sections.
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Xinzhe
authors.initials: X
authors.lastname: Yu
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Lie
authors.initials: L
authors.lastname: Yao
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Di
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Hang
authors.initials: H
authors.lastname: He
authors.affiliation: Key Laboratory of Smart Drug Delivery, Fudan University, Shanghai, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Smart Drug Delivery, Fudan University, Shanghai, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Fu
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jin
authors.affiliation: Pancreatic Surgery Department, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D051379:Mice; D009362:Neoplasm Metastasis / Q000000981:diagnostic imaging*; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011897:Random Allocation
chemical_list: D017319:Photosensitizing Agents