title: Cellular Synthesis of Protein Catenanes.
abstract: Direct cellular production of topologically complex proteins is of great interest both in supramolecular chemistry and protein engineering. We describe the first cellular synthesis of protein catenanes through the use of the p53 dimerization domain to guide the intertwining of two protein chains and SpyTag-SpyCatcher chemistry for efficient cyclization. The catenane topology was unambiguously proven by SDS-PAGE, SEC, and partial digestion experiments and was shown to confer enhanced stability toward trypsin digestion relative to monomeric control mutants. The assembly-reaction synergy enabled by protein folding and genetically encoded protein chemistry offers a convenient yet powerful approach for creating mechanically interlocked, complex protein topologies in vivo.
authors.affiliation: Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, P.R. China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
authors.affiliation: Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, P.R. China. wenbin@pku.edu.cn.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Zhang
mesh_terms: D000873:Anthracenes / Q000378:metabolism*; D003500:Cyclization; D019281:Dimerization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry
chemical_list: D000873:Anthracenes; D016159:Tumor Suppressor Protein p53; C467767:catenane
keywords: catenanes; protein folding; protein structures; supramolecular chemistry; topology