title: Correlation between microRNA-421 expression level and prognosis of gastric cancer.
abstract: The expression of microRNA-421 (miR-421) is significantly elevated in gastric carcinoma cells, thus may play an important role in tumor occurrence. This study thus aimed to further illustrate the correlation between miR-421 expression level and the progression and prognosis of gastric carcinoma. A total of 96 gastric carcinoma tissue samples were quantified for miR-421 expression level using quantitative PCR (qPCR) method. Kaplan-Meier survival curve was further deployed to analyze the postoperative survival of all patients. No significant correlation existed between miR-421 level and general information of patients such as age, sex, tumor size, location, invasion depth, TNM stage, differentiation stage and metastasis. However, miR-421 was significantly up-regulated in those tumors with lymph node metastasis (P<0.05), while those tumors with no lymph node metastasis had normal miR-421 level as those in control group (P>0.05). These results suggested the correlation between miR-421 up-regulation and lymph node metastasis. Those patients with high miR-421 expression had significantly shorter survival time compared to normal miR-421 patients (median: 37.34 months vs. 54.23 months, P<0.01), suggesting the correlation between miR-421 expression and prognosis of gastric cancer. MiR-421 level is correlated with lymph node metastasis and prognosis of gastric carcinoma, and is worth for further investigations.
authors.affiliation: Department of Anatomy, College of Basic Medical Sciences, Jilin University Changchun, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Colorectal and Anal surgery, China-Japan Union Hospital, Jilin University Changchun, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Gastrointestinal Colorectal and Anal surgery, China-Japan Union Hospital, Jilin University Changchun, China.
authors.forename: Defeng
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Gastrointestinal Colorectal and Anal surgery, China-Japan Union Hospital, Jilin University Changchun, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Colorectal and Anal surgery, China-Japan Union Hospital, Jilin University Changchun, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C547665:MIRN421 microRNA, human; D035683:MicroRNAs
keywords: Gastric carcinoma; MicroRNA-421; correlation analysis; prognosis prediction
references.citation: Breast Cancer Res Treat. 2011 Dec;130(3):735-45
references.pmid: 21409395
references.citation: Eur J Cancer. 2011 Nov;47(17):2552-9
references.pmid: 21684154
references.citation: Tumour Biol. 2012 Dec;33(6):2349-55
references.pmid: 22926798
references.citation: Science. 2002 Dec 6;298(5600):1912-34
references.pmid: 12471243
references.citation: Biomarkers. 2015 Feb;20(1):58-63
references.pmid: 25510566
references.citation: Biochem Biophys Res Commun. 2013 Jun 14;435(4):745-50
references.pmid: 23707940
references.citation: Nat Genet. 2005 May;37(5):495-500
references.pmid: 15806104
references.citation: Nat Methods. 2006 Nov;3(11):881-6
references.pmid: 17060911
references.citation: Breast Cancer Res. 2009;11(1):R13
references.pmid: 19243594
references.citation: J Gastroenterol. 2009;44(6):556-61
references.pmid: 19363643
references.citation: Int J Cancer. 2010 Jan 1;126(1):114-24
references.pmid: 19569050
references.citation: J Gastroenterol. 2010;45(1):17-23
references.pmid: 19802518
references.citation: Lancet Oncol. 2010 Feb;11(2):136-46
references.pmid: 20022810
references.citation: PLoS One. 2010;5(3):e9500
references.pmid: 20209161
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6982-7
references.pmid: 20351277
references.citation: Exp Mol Med. 2010 Apr 30;42(4):233-44
references.pmid: 20177143
references.citation: Cell Mol Life Sci. 2010 Aug;67(15):2605-18
references.pmid: 20238234
references.citation: Gynecol Oncol. 2011 Jan;120(1):56-62
references.pmid: 21035172
references.citation: Eur J Immunol. 2011 Feb;41(2):549-51
references.pmid: 21268023
references.citation: Cancer Res. 2011 Nov 1;71(21):6836-47
references.pmid: 21890822
references.citation: Int J Oncol. 2012 Jun;40(6):1839-48
references.pmid: 22426824