title: Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-Chaperone System.
abstract: Protein aggregation is a common problem in protein biochemistry and is linked to many cellular pathologies and human diseases. The molecular chaperone ClpB can resolubilize and reactivate aggregated proteins. This unit describes the procedure for following reactivation of an aggregated enzyme glucose-6-phosphate dehydrogenase mediated by ClpB from Escherichia coli in cooperation with another molecular chaperone, DnaK. The procedures for purification of these chaperones are also described.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas.
authors.forename: Michal
authors.initials: M
authors.lastname: Zolkiewski
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana.
authors.forename: Liudmila S
authors.initials: LS
authors.lastname: Chesnokova
authors.affiliation: Department of Biochemistry and Molecular Biology and Department of Pharmacology, Toxicology and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, Louisiana.
authors.forename: Stephan N
authors.initials: SN
authors.lastname: Witt
mesh_terms: D049071:Endopeptidase Clp; D004789:Enzyme Activation; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066329:Protein Aggregates; D012995:Solubility
chemical_list: D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D066329:Protein Aggregates; D005954:Glucosephosphate Dehydrogenase; D049071:Endopeptidase Clp; C063842:dnaK protein, E coli; C069775:ClpB protein, E coli
keywords: ClpB; DnaK; molecular chaperone; protein aggregation; protein misfolding
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