title: Molecular diversity of bacteria in commercially available "Spirulina" food supplements.
abstract: The cyanobacterium Arthrospira is among the most well-known food supplements worldwide known as "Spirulina." While it is a widely recognized health-promoting natural product, there are no reports on the molecular diversity of commercially available brands of "Spirulina" supplements and the occurrence of other cyanobacterial and heterotrophic bacterial microorganisms in these products. In this study, 454-pyrosequencing analysis of the total bacterial occurrence in 31 brands of "Spirulina" dietary supplements from the Greek market was applied for the first time. In all samples, operational taxonomic units (OTUs) of Arthrospira platensis were the predominant cyanobacteria. Some products contained additional cyanobacterial OTUs including a few known potentially toxic taxa. Moreover, 469 OTUs were detected in all 31 products collectively, with most of them being related to the Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria and Verrucomicrobia. All samples included heterotrophic bacterial OTUs, ranging from 9-157 per product. Among the most common OTUs were ones closely related to taxa known for causing health issues (i.e., Pseudomonas, Flavobacterium, Vibrio, Aeromonas, Clostridium, Bacillus, Fusobacterium, Enterococcus). The observed high cyanobacterial and heterotrophic bacterial OTUs richness in the final product is a point for further research on the growth and processing of Arthrospira biomass for commercial purposes.
authors.affiliation: Department of Nutrition and Dietetics, Alexander Technological Educational Institute of Thessaloniki , Thessaloniki , Greece.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Vardaka
authors.affiliation: Department of Ichthyology and Aquatic Environment, University of Thessaly , Volos , Greece.
authors.forename: Konstantinos A
authors.initials: KA
authors.lastname: Kormas
authors.affiliation: Department of Nutrition and Dietetics, Alexander Technological Educational Institute of Thessaloniki, Thessaloniki, Greece; School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Matina
authors.initials: M
authors.lastname: Katsiapi
authors.affiliation: School of Biology, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors.forename: Savvas
authors.initials: S
authors.lastname: Genitsaris
authors.affiliation: School of Biology, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Moustaka-Gouni
keywords: Arthrospira; Bacteria; Cyanobacteria; Food supplements; Pyrosequencing; Spirulina
references.citation: Am J Gastroenterol. 2002 Dec;97(12):3212-3
references.pmid: 12492223
references.citation: Nucleic Acids Res. 2007;35(21):7188-96
references.pmid: 17947321
references.citation: Phytomedicine. 2008 Jun;15(6-7):525-7
references.pmid: 18434120
references.citation: BMC Microbiol. 2008 Jul 24;8:125
references.pmid: 18652685
references.citation: J Hepatol. 2009 Jan;50(1):111-7
references.pmid: 19010564
references.citation: Nat Methods. 2009 Sep;6(9):639-41
references.pmid: 19668203
references.citation: Environ Microbiol. 2010 Jan;12(1):118-23
references.pmid: 19725865
references.citation: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references.pmid: 19801464
references.citation: Mol Ecol. 2010 Mar;19 Suppl 1:21-31
references.pmid: 20331767
references.citation: PLoS One. 2011 Apr 04;6(4):e18169
references.pmid: 21483744
references.citation: J Appl Phycol. 2011 Apr;23(2):265-269
references.pmid: 21544188
references.citation: Nucleic Acids Res. 2013 Jan 7;41(1):e1
references.pmid: 22933715
references.citation: PLoS One. 2014 Feb 07;9(2):e87624
references.pmid: 24516555
references.citation: J Phycol. 2008 Apr;44(2):260-8
references.pmid: 27041182
references.citation: Int J Food Microbiol. 1998 Jun 30;42(1-2):1-7
references.pmid: 9706793