title: Marinobacter strain NNA5, a newly isolated and highly efficient aerobic denitrifier with zero N2O emission.
abstract: An efficient aerobic denitrification bacterium, strain NNA5, was isolated and identified as Marinobacter sp. NNA5. NNA5 did not perform heterotrophic nitrification. GC/IRMS analysis revealed that (15)N2 was produced from Na(15)NO2 and K(15)NO3. GC/MS and quantitative analyses showed that no N2O emission occurred when nitrite or nitrate was used as substrate. Single factor experiments indicated that optimal conditions for aerobic denitrification were: sodium succinate or sodium pyruvate as carbon source, temperature 35 °C, NaCl concentration 2-4%, C/N ratio 6-8, pH 7.5, rotation speed 150 rpm (giving dissolved oxygen concentration 6.08 mg/L), NO3(-)-N concentration ranging from 140 to 700 mg/L. NNA5 displayed highly efficient aerobic denitrifying ability, with maximal NO3(-)-N removal rate 112.8 mg/L/d. In view of its ability to perform aerobic denitrification with zero N2O emission, NNA5 has great potential for future application in aerobic denitrification processes in industrial and aquaculture wastewater treatment systems.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Ai
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Miao
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: liuzhp@sun.im.ac.cn.
authors.forename: Zhi-Pei
authors.initials: ZP
authors.lastname: Liu
mesh_terms: D000332:Aerobiosis; D017756:Aquaculture; D058440:Denitrification; D053520:Marinobacter / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009609:Nitrous Oxide / Q000032:analysis*; D013696:Temperature; D062065:Wastewater
chemical_list: D009566:Nitrates; D009573:Nitrites; D009609:Nitrous Oxide; D062065:Wastewater
keywords: Aerobic denitrification; Marinobacter strain NNA5; N(2)O emission; Nitrogen removal; Wastewater treatment