title: Caviibacter abscessus gen. nov., sp. nov., a member of the family Leptotrichiaceae isolated from guinea pigs (Cavia porcellus).
abstract: A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was originally isolated from the mandibular lymph node of a guinea pig and deposited as Streptobacillus moniliformis CCUG 39713 in 1998. A second strain, 151011837, was isolated from an identical lesion in a guinea pig in Germany in 2015. On the basis of 16S rRNA gene sequence analyses, these strains displayed highest sequence similarities with Sneathia sanguinegens NTS65407T (93.4%) and 'Sneathia amnii' Sn35 (93.2%), followed by Streptobacillus moniliformis DSM 12112T (91.3%), 'Streptobacillus ratti' OGS16 (91.2%), Streptobacillus notomytis AHL370-1T (91.0%), Streptobacillus hongkongensis HKU33T (90.9%) and Streptobacillus felis 131000547T (90.9%). Levels of sequence similarity with all other members of the family Leptotrichiaceae were <89%. Results of phylogenetic analyses of strains CCUG 39713T and 151011837, based on gyrB, groEL and recA nucleotide and deduced amino acid sequences, were highly similar, as the topologies of all trees were virtually identical. DNA relatedness values derived from average nucleotide identities calculated for comparisons between strain CCUG 39713T and the type strains of Sneathia sanguinegens and Streptobacillus moniliformis, respectively, were 72.05 and 70.42%. The genomes of CCUG39713T and 151011837 shared 99.57% average nucletide identity. The chemotaxonomic and physiological data for strains CCUG 39713T and 151011837 were in congruence with other closely related members of the family Leptotrichiaceae, with highly similar enzyme activities and fatty acid profiles. Matrix-assisted laser desorption ionization time-of-flight MS analysis was capable of clearly discriminating strains CCUG 39713T and 151011837 from all taxa of the family Leptotrichiaceae with validly published names. On the basis of these data, the novel taxon Caviibacter abscessus gen. nov., sp. nov. is proposed. The type strain of Caviibacter abscessus is CCUG 39713T (=DSM 101949T); 151011837 (DSM 101950) is an additional strain of the species.
authors.affiliation: Landesbetrieb Hessisches Landeslabor, D-35392 Giessen, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Ewers
authors.affiliation: Robert Koch-Institut, D-13353 Berlin, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Semmler
authors.affiliation: Kleintierpraxis Dr. Drescher, D-70599 Stuttgart, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Drescher
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045182:Fusobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D005858:Germany; D006168:Guinea Pigs / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S