title: Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2.
abstract: MicroRNAs (miRNAs) are a class of small non-coding RNAs and closely related to the pathogenesis of cancers. Increasing evidence indicates that miR-30a plays a profound role during the development of cancers. However, the functions of miR-30a in non-small-cell lung cancer (NSCLC) are still ambiguous. Here we found that miR-30a was decreased in lung adenocarcinoma A549 cells and in tissue samples from 14 patients by qRT-PCR, and also found that overexpression of miR-30a in A549 cells inhibited migration and invasion but not cell proliferation and cell cycle progression by wound-healing assay, matrigel invasion assay, MTS-based cell proliferation assay, and flow cytometry-based cell cycle analysis, respectively. We further explored the potential mechanism of miR-30a-mediated gene regulation in lung adenocarcinoma cell lines. EYA2 is a predicted target of miR-30a, and it has been found that EYA2 expression is inhibited by miR-30a in breast cancer cells. We demonstrated that EYA2 is a direct target of miR-30a by using the dual-luciferase reporter assay in A549 cells and showed that EYA2 protein levels are inversely correlated with miR-30a expression in A549 and BEAS-2B cells. In addition, we also confirmed the rescue effects of EYA2 overexpression in A549 cells by cotransfection with EYA2 expression vector and miR-30a mimics. Taken together, our results demonstrate that overexpression of miR-30a in lung adenocarcinoma A549 cells can inhibit cell migration and invasion, which is partially attributed to the decrease of EYA2 expression. Our findings suggest that miR-30a may be used as a new potential target for the treatment of lung adenocarcinoma in the future.
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Yuncang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Shangyong
authors.initials: S
authors.lastname: Zheng
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Xiang
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Tangxin
authors.initials: T
authors.lastname: Gao
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Pengzhan
authors.initials: P
authors.lastname: Ran
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xie
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, China chjxiao@ynu.edu.cn.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Xiao
mesh_terms: D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D047908:Intracellular Signaling Peptides and Proteins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; C106938:EYA2 protein, human; D017027:Protein Tyrosine Phosphatases
keywords: EYA2; invasion; lung adenocarcinoma; miR-30a; migration
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