title: Trichoderma longibrachiatum Evx1 is a fungal biocatalyst suitable for the remediation of soils contaminated with diesel fuel and polycyclic aromatic hydrocarbons.
abstract: Trichoderma sp. strain Evx1 was isolated from a semi-deciduous forest soil in Southern Italy. It decolorizes polynuclear organic dyes and tolerates high concentrations of phenanthrene, anthracene, fluoranthene, and pyrene. The ability of this ascomycete fungus to degrade polycyclic aromatic hydrocarbons was verified in vitro and confirmed by its strong phenoloxidase activity in the presence of gallic acid. Phylogenetic characterization of Trichoderma sp. Evx1 positioned this strain within the species Trichoderma longibrachiatum. The potential use of this species for the bioremediation of contaminated environmental matrices was tested by inoculating diesel-spiked soil with a dense mycelial suspension. The biodegradation percentage of the C12-40 hydrocarbon fraction in the inoculated soil rose to 54.2 ± 1.6 %, much higher than that in non-inoculated soil or soil managed solely by a combination of watering and aeration. The survival and persistence of T. longibrachiatum Evx1 throughout the bioremediation trial was monitored by PCR-DGGE analysis. The fungal strain was still present in the soil 30 days after bioaugmentation. These findings indicate that T. longibrachiatum Evx1 may be a suitable inoculum in bioremediation protocols for the reclamation of soils contaminated by complex mixtures of hydrocarbons.
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Andreolli
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. silvia.lampis@univr.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lampis
authors.affiliation: Eurovix SpA - Viale Enrico Mattei 17, 24060, Entratico, BG, Italy.
authors.forename: Pierlorenzo
authors.initials: P
authors.lastname: Brignoli
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. giovanni.vallini@univr.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vallini
mesh_terms: D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005449:Fluorenes; D065928:Forests; D005742:Gasoline / Q000032:analysis* / Q000382:microbiology; D006838:Hydrocarbons / Q000378:metabolism; D007558:Italy; D010616:Phenanthrenes; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D014242:Trichoderma / Q000378:metabolism / Q000502:physiology*
chemical_list: D005449:Fluorenes; D005742:Gasoline; D006838:Hydrocarbons; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants; C007738:fluoranthene; C031181:phenanthrene
keywords: Bioremediation; Diesel fuel; Hydrocarbon polluted soil; Polycyclic aromatic hydrocarbons; Polynuclear organic dye decolorization; Trichoderma longibrachiatum Evx1
references.citation: Fungal Genet Biol. 2012 May;49(5):358-68
references.pmid: 22405896
references.citation: Fundam Appl Toxicol. 1989 Oct;13(3):545-53
references.pmid: 2612787
references.citation: Mycobiology. 2008 Jun;36(2):114-20
references.pmid: 23990745
references.citation: J Hazard Mater. 2009 Aug 30;168(1):406-15
references.pmid: 19286314
references.citation: Microbiology. 2008 Nov;154(Pt 11):3447-59
references.pmid: 18957598
references.citation: Sci Total Environ. 2014 Apr 1;476-477:561-7
references.pmid: 24496029
references.citation: J Microbiol Methods. 2014 Feb;97:56-62
references.pmid: 24374215
references.citation: Environ Sci Pollut Res Int. 2015 Jan;22(2):1034-42
references.pmid: 25106516
references.citation: J Microbiol Methods. 2006 Apr;65(1):63-75
references.pmid: 16102860
references.citation: Chemosphere. 2013 Apr;91(5):711-6
references.pmid: 23402919
references.citation: Appl Microbiol Biotechnol. 2010 Jul;87(3):787-99
references.pmid: 20461510
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Bioresour Technol. 2001 Sep;79(3):251-62
references.pmid: 11499579
references.citation: Curr Microbiol. 2011 Feb;62(2):351-7
references.pmid: 20644933
references.citation: Environ Pollut. 2003;125(3):361-8
references.pmid: 12826414
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: J Hazard Mater. 2012 Oct 30;237-238:186-93
references.pmid: 22959131
references.citation: J Appl Microbiol. 2010 Jun;108(6):2030-40
references.pmid: 19922595
references.citation: PLoS One. 2010 Feb 12;5(2):e9191
references.pmid: 20169200
references.citation: J Ind Microbiol Biotechnol. 2009 Oct;36(10 ):1249-56
references.pmid: 19543759
references.citation: Bioprocess Biosyst Eng. 2012 Jun;35(5):851-5
references.pmid: 22170303
references.citation: Fungal Genet Biol. 2010 May;47(5):458-67
references.pmid: 20144725
references.citation: Stud Mycol. 2009;63:1-91
references.pmid: 19826500
references.citation: Bioresour Technol. 2011 Sep;102(17):8063-70
references.pmid: 21683591
references.citation: J Zhejiang Univ Sci B. 2008 Oct;9(10):753-63
references.pmid: 18837102
references.citation: Fungal Divers. 2012 Jul 1;55(1):77-108
references.pmid: 22956918
references.citation: J Environ Manage. 2012 Mar;95 Suppl:S291-9
references.pmid: 20869805
references.citation: Biodegradation. 2015 Jun;26(3):259-69
references.pmid: 25929330
references.citation: Curr Opin Microbiol. 2005 Jun;8(3):268-75
references.pmid: 15939349
references.citation: Environ Microbiol. 2000 Apr;2(2):179-90
references.pmid: 11220304
references.citation: J Microbiol Methods. 2000 Dec 15;43(2):133-51
references.pmid: 11121612
references.citation: FEMS Microbiol Lett. 2013 Oct;347(2):123-9
references.pmid: 23909277
references.citation: J Environ Manage. 2015 Apr 15;153:121-31
references.pmid: 25688477
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: FEMS Microbiol Lett. 2011 Jun;319(1):11-8
references.pmid: 21388438