title: Identification of an Interfering Ligand Aptamer for EphB2/3 Receptors.
abstract: The Eph receptors are transmembrane proteins that belong to the receptor tyrosine kinases superfamily. Elevated Eph/ephrin expression levels have been associated with angiogenesis and tumor vasculature in many types of human cancers, including breast, lung, and prostate cancers, melanoma, and leukemia. In glioblastoma (GBM), the dysregulated expression of Eph receptors and of corresponding ephrin ligands has been associated with higher tumor grade and poor prognosis making them effective targets for therapeutic drugs. In this study, we describe the GL43.T, an anti-Eph aptamer, able to bind at high-affinity EphB3 and EphB2. Moreover, the GL43.T aptamer inhibits the glioma cell vitality and interferes with ephrine-B1 inhibition of chemotactic serum-stimulated cell migration. GL43.T aptamer represents a promising therapeutic molecule for EphB3-dependent cancers.
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale , CNR, Naples, Italy .
authors.forename: Paola
authors.initials: P
authors.lastname: Amero
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale , CNR, Naples, Italy .
authors.forename: Carla Lucia
authors.initials: CL
authors.lastname: Esposito
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale , CNR, Naples, Italy .
authors.forename: Anna
authors.initials: A
authors.lastname: Rienzo
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale , CNR, Naples, Italy .
authors.forename: Fortunato
authors.initials: F
authors.lastname: Moscato
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale , CNR, Naples, Italy .
authors.forename: Silvia
authors.initials: S
authors.lastname: Catuogno
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale , CNR, Naples, Italy .
authors.forename: Vittorio
authors.initials: V
authors.lastname: de Franciscis
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000235:genetics* / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites; D002465:Cell Movement; D049109:Cell Proliferation; D004354:Drug Screening Assays, Antitumor; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D034622:RNA Interference; D036183:Receptor, EphB2 / Q000096:biosynthesis / Q000235:genetics*; D036223:Receptor, EphB3 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; C586310:EPHB2 protein, human; D036183:Receptor, EphB2; D036223:Receptor, EphB3