title: 4-Hydroxy cinnamic acid as mushroom preservation: Anti-tyrosinase activity kinetics and application.
abstract: Tyrosinase is a key enzyme in post-harvest browning of fruit and vegetable. To control and inhibit its activity is the most effective method for delaying the browning and extend the shelf life. In this paper, the inhibitory kinetics of 4-hydroxy cinnamic acid on mushroom tyrosinase was investigated using the kinetics method of substrate reaction. The results showed that the inhibition of tyrosinase by 4-hydroxy cinnamic acid was a slow, reversible reaction with fractional remaining activity. The microscopic rate constants were determined for the reaction on 4-hydroxy cinnamic acid with tyrosinase. Furthermore, the molecular docking was used to simulate 4-hydroxy cinnamic acid dock with tyrosinase. The results showed that 4-hydroxy cinnamic acid interacted with the enzyme active site mainly through the hydroxy competed with the substrate hydroxy group. The cytotoxicity study of 4-hydroxy cinnamic acid indicated that it had no effects on the proliferation of normal liver cells. Moreover, the results of effects of 4-hydroxy cinnamic acid on the preservation of mushroom showed that it could delay the mushroom browning. These results provide a comprehensive underlying the inhibitory mechanisms of 4-hydroxy cinnamic acid and its delaying post-harvest browning, that is beneficial for the application of this compound.
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Hu
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China; Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China.
authors.forename: Qing-Xi
authors.initials: QX
authors.lastname: Chen
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Huan-Juan
authors.initials: HJ
authors.lastname: Gao
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Yu
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China.
authors.forename: Chong-Ling
authors.initials: CL
authors.lastname: Yan
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361005, China. Electronic address: qwang@xmu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000201:enzymology* / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D003373:Coumaric Acids / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D005519:Food Preservation / Q000379:methods*; D061353:Food Storage; D007700:Kinetics; D008099:Liver / Q000166:cytology; D062105:Molecular Docking Simulation; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011422:Propionates; D011487:Protein Conformation; D013696:Temperature
chemical_list: D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D011422:Propionates; D014442:Monophenol Monooxygenase; C495469:p-coumaric acid
keywords: 4-Hydroxy cinnamic acid; Inhibitory kinetics; Mushroom preservation