title: DACH1 inhibits the proliferation and invasion of lung adenocarcinoma through the downregulation of peroxiredoxin 3.
abstract: In this study, we found the expression of Dachshund 1 (DACH1) is downregulated while peroxiredoxin 3 (PRX3) upregulated in both lung adenocarcinoma tissues and cells. Transfection of DACH1 can significantly downregulate PRX3 expression in targeting lung adenocarcinoma cells. Further experimental results demonstrated the evidence that overexpression of DACH1 resulted in significant retardation of in vitro proliferation and invasion of lung adenocarcinoma cells. Direct upregulation of PRX3 by co-transfection of PRX3 messenger RNA (mRNA) can prevent the above alteration caused by DACH1 transfection. Besides, lower DACH1 expression significantly correlated with tumor diameter and tumor invasion in all the 36 patients diagnosed with lung adenocarcinoma in our hospital during the past months. In conclusion, DACH1 can inhibit the proliferation and invasion of lung adenocarcinoma through the downregulation of PRX3. Decreased expression of DACH1 is involved in the initiation and development of lung cancer, which might be an adverse prognostic factor of lung adenocarcinoma.
authors.affiliation: Department of Cardiothoracic Surgery, Changhai Hospital Affiliated to the Second Military Medical University, 168 Changhai Road, 200433, Shanghai, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Diagnosis, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wu
authors.affiliation: International Joint Cancer Institute, Translational Medicine Research Institute, The Second Military Medical University, Shanghai, China.
authors.forename: Huafei
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Cardiothoracic Surgery, Changhai Hospital Affiliated to the Second Military Medical University, 168 Changhai Road, 200433, Shanghai, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Cardiothoracic Surgery, Changhai Hospital Affiliated to the Second Military Medical University, 168 Changhai Road, 200433, Shanghai, People's Republic of China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Changhai Hospital Affiliated to the Second Military Medical University, 168 Changhai Road, 200433, Shanghai, People's Republic of China. drzhaotiejun@126.com.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Cardiothoracic Surgery, Changzheng Hospital Affiliated to the Second Military Medical University, 415 Fengyang Road, 200433, Shanghai, People's Republic of China. jibinx@yahoo.com.
authors.forename: Jibin
authors.initials: J
authors.lastname: Xu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D061105:Peroxiredoxin III / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; C486747:DACH1 protein, human; D005136:Eye Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III
keywords: Cell cycle; DACH1; Lung adenocarcinoma; PRX3
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