title: A gradient of maternal Bicaudal-C controls vertebrate embryogenesis via translational repression of mRNAs encoding cell fate regulators.
abstract: Vertebrate Bicaudal-C (Bicc1) has important biological roles in the formation and homeostasis of multiple organs, but direct experiments to address the role of maternal Bicc1 in early vertebrate embryogenesis have not been reported. Here, we use antisense phosphorothioate-modified oligonucleotides and the host-transfer technique to eliminate specifically maternal stores of both bicc1 mRNA and Bicc1 protein from Xenopus laevis eggs. Fertilization of these Bicc1-depleted eggs produced embryos with an excess of dorsal-anterior structures and overexpressed organizer-specific genes, indicating that maternal Bicc1 is crucial for normal embryonic patterning of the vertebrate embryo. Bicc1 is an RNA-binding protein with robust translational repression function. Here, we show that the maternal mRNA encoding the cell-fate regulatory protein Wnt11b is a direct target of Bicc1-mediated repression. It is well established that the Wnt signaling pathway is crucial to vertebrate embryogenesis. Thus, the work presented here links the molecular function of Bicc1 in mRNA target-specific translation repression to its biological role in the maternally controlled stages of vertebrate embryogenesis.
authors.affiliation: Department of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Sookhee
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Susanne
authors.initials: S
authors.lastname: Blaser
authors.affiliation: Department of Biology, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Marchal
authors.affiliation: Department of Biology, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Douglas W
authors.initials: DW
authors.lastname: Houston
authors.affiliation: Department of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA mdsheets@wisc.edu.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Sheets
mesh_terms: D000818:Animals; D019070:Cell Lineage; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D035683:MicroRNAs / Q000378:metabolism; D009154:Mutation; D016376:Oligonucleotides, Antisense / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D023121:RNA, Messenger, Stored / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic; D051153:Wnt Proteins / Q000378:metabolism*; D029867:Xenopus Proteins / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: C453440:BICC1 protein, Xenopus; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D023121:RNA, Messenger, Stored; D016601:RNA-Binding Proteins; D051153:Wnt Proteins; D029867:Xenopus Proteins; C512785:wnt11b protein, Xenopus
keywords: Maternal mRNA; Regulated translation; Wnt; Xenopus
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