title: Fish Oil Supplementation Increases Event-Related Posterior Cingulate Activation in Older Adults with Subjective Memory Impairment.
abstract: OBJECTIVE
  To determine the effects of long-chain omega-3 (LCn-3) fatty acids found in fish oil, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on cortical blood oxygen level-dependent (BOLD) activity during a working memory task in older adults with subjective memory impairment.
  
  
  METHODS
  Randomized, double-blind, placebo-controlled study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Healthy older adults (62-80 years) with subjective memory impairment, but not meeting criteria for mild cognitive impairment or dementia.
  
  
  METHODS
  Fish oil (EPA+DHA: 2.4 g/d, n=11) or placebo (corn oil, n=10) for 24 weeks.
  
  
  METHODS
  Cortical BOLD response patterns during performance of a sequential letter n-back working memory task were determined at baseline and week 24 by functional magnetic resonance imaging (fMRI).
  
  
  RESULTS
  At 24 weeks erythrocyte membrane EPA+DHA composition increased significantly from baseline in participants receiving fish oil (+31%, p ≤ 0.0001) but not placebo (-17%, p=0.06). Multivariate modeling of fMRI data identified a significant interaction among treatment, visit, and memory loading in the right cingulate (BA 23/24), and in the right sensorimotor area (BA 3/4). In the fish oil group, BOLD increases at 24 weeks were observed in the right posterior cingulate and left superior frontal regions during memory loading. A region-of-interest analysis indicated that the baseline to endpoint change in posterior cingulate cortex BOLD activity signal was significantly greater in the fish oil group compared with the placebo group during the 1-back (p=0.0003) and 2-back (p=0.0005) conditions. Among all participants, the change in erythrocyte EPA+DHA during the intervention was associated with performance in the 2-back working memory task (p = 0.01), and with cingulate BOLD signal during the 1-back (p = 0.005) with a trend during the 2-back (p = 0.09). Further, cingulate BOLD activity was related to performance in the 2-back condition.
  
  
  CONCLUSIONS
  Dietary fish oil supplementation increases red blood cell omega-3 content, working memory performance, and BOLD signal in the posterior cingulate cortex during greater working memory load in older adults with subjective memory impairment suggesting enhanced neuronal response to working memory challenge.
authors.affiliation: Robert Krikorian, PhD, Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati Academic Health Center, PO Box 670559, Cincinnati, OH 45267-0559, PH: 513-558-2455, FX: 513-558-0877, EM: robert.krikorian@uc.edu.
authors.forename: E L
authors.initials: EL
authors.lastname: Boespflug
authors.forename: R K
authors.initials: RK
authors.lastname: McNamara
authors.forename: J C
authors.initials: JC
authors.lastname: Eliassen
authors.forename: M D
authors.initials: MD
authors.lastname: Schidler
authors.forename: R
authors.initials: R
authors.lastname: Krikorian
mesh_terms: D000368:Aged; D000818:Animals; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D004311:Double-Blind Method; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005395:Fish Oils / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D006179:Gyrus Cinguli / Q000187:drug effects*; D006801:Humans; D008297:Male; D008569:Memory Disorders / Q000097:blood / Q000188:drug therapy*; D008570:Memory, Short-Term / Q000187:drug effects*; D008875:Middle Aged; D010100:Oxygen / Q000097:blood
chemical_list: D005395:Fish Oils; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D010100:Oxygen
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