title: Characterization of extracellular amylase produced by haloalkalophilic strain Kocuria sp. HJ014.
abstract: The haloalkaliphilic bacterium Kocuria sp. (HJ014) has the ability to produce extracellular amylase. The aim of this study was to purify and characterize this protein. The amylase enzyme with a specific activity of 753,502 U/mg was purified 5.7- fold using Sepharose 4B and Sephacryl S-300 gel filtration columns. The molecular weight of the enzyme was 45,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The amylase showed maximum activity at pH 9 and 50°C in the presence of 3.5 M NaCl. The Km was 3.0 mg/ml and Vmax 90.09 U/ml. It was found that extracellular amylase from Kocuria sp. has a high industrial potential.
authors.affiliation: a Instituto de Ingeniería y Tecnología , Universidad Autónoma de Ciudad Juárez , Chihuahua , Mexico.|b Biotecnología y Ciencias Alimentarias , Instituto Tecnológico de Sonora , Obregón , Mexico.
authors.forename: Marisela Y
authors.initials: MY
authors.lastname: Soto-Padilla
authors.affiliation: b Biotecnología y Ciencias Alimentarias , Instituto Tecnológico de Sonora , Obregón , Mexico.
authors.forename: Pablo
authors.initials: P
authors.lastname: Gortáres-Moroyoqui
authors.affiliation: b Biotecnología y Ciencias Alimentarias , Instituto Tecnológico de Sonora , Obregón , Mexico.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Cira-Chávez
authors.affiliation: c Biotechnologie des Champignons Filamenteux , INRA , Marseille , France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Levasseur
authors.affiliation: d Cinvestav , ABACUS , Mexico City , Mexico.
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
authors.affiliation: b Biotecnología y Ciencias Alimentarias , Instituto Tecnológico de Sonora , Obregón , Mexico.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Estrada-Alvarado
mesh_terms: D000681:Amylases / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008835:Micrococcaceae / Q000201:enzymology*; D008970:Molecular Weight
chemical_list: D000681:Amylases
keywords: Haloalkaliphilic; enzyme molecular weight; industrial potential; specific activity