title: Effects of cell phone radiation on lipid peroxidation, glutathione and nitric oxide levels in mouse brain during epileptic seizure.
abstract: The objective of the this study was to evaluate the effects of cellular phone radiation on oxidative stress parameters and oxide levels in mouse brain during pentylenetetrazole (PTZ) induced epileptic seizure. Eight weeks old mice were used in the study. Animals were distributed in the following groups: Group I: Control group treated with PTZ, Group II: 15min cellular phone radiation+PTZ treatment+30min cellular phone radiation, Group III: 30min cellular phone radiation+PTZ treatment+30min cellular phone radiation. The RF radiation was produced by a 900MHz cellular phone. Lipid peroxidation, which is the indicator of oxidative stress was quantified by measuring the formation of thiobarbituric acid reactive substances (TBARS). The glutathione (GSH) levels were determined by the Ellman method. Tissue total nitric oxide (NOx) levels were obtained using the Griess assay. Lipid peroxidation and NOx levels of brain tissue increased significantly in group II and III compared to group I. On the contrary, GSH levels were significantly lower in group II and III than group I. However, no statistically significant alterations in any of the endpoints were noted between group II and Group III. Overall, the experimental findings demonstrated that cellular phone radiation may increase the oxidative damage and NOx level during epileptic activity in mouse brain.
authors.affiliation: Department of Biophysics, Gazi University, Ankara, Turkey. Electronic address: mericarda@yahoo.com.
authors.forename: Meric Arda
authors.initials: MA
authors.lastname: Esmekaya
authors.affiliation: Department of Biophysics, Gazi University, Ankara, Turkey.
authors.forename: Mehmet Zahid
authors.initials: MZ
authors.lastname: Tuysuz
authors.affiliation: Department of Biophysics, Gazi University, Ankara, Turkey.
authors.forename: Arın
authors.initials: A
authors.lastname: Tomruk
authors.affiliation: Department of Biophysics, Gazi University, Ankara, Turkey.
authors.forename: Ayse G
authors.initials: AG
authors.lastname: Canseven
authors.affiliation: Department of Neurosurgery, Baskent University, Alanya Training and Research Hospital, Antalya, TURKEY.
authors.forename: Engin
authors.initials: E
authors.lastname: Yücel
authors.affiliation: Department of Medical Pharmacology, Kırıkkale University, Kırıkkale, TURKEY.
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Aktuna
authors.affiliation: Department of Neurosurgery, Kırıkkale University, Kırıkkale, TURKEY.
authors.forename: Semih
authors.initials: S
authors.lastname: Keskil
authors.affiliation: Department of Biophysics, Gazi University, Ankara, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Seyhan
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000528:radiation effects*; D040421:Cell Phone; D005260:Female; D005978:Glutathione / Q000378:metabolism / Q000528:radiation effects*; D015227:Lipid Peroxidation / Q000502:physiology / Q000528:radiation effects*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism / Q000528:radiation effects*; D012640:Seizures / Q000378:metabolism
chemical_list: D009569:Nitric Oxide; D005978:Glutathione
keywords: Cell phone; Mice; Pentylenetetrazole; Radiation; Seizure