title: shot regulates the microtubule reorganization required for localization of axis-determining mRNAs during oogenesis.
abstract: The Drosophila mid-oogenesis stages are notable as the time when most maternal mRNAs become localized at discrete regions of the oocyte. Microtubule rearrangement occurs during this period and is critical for the localization of axis-determining maternal mRNAs. We have identified shot as a key player in establishing the cytoskeletal arrangement required for the spatial localization of axis-determining maternal mRNAs. We also found that the spatial distribution of the Shot protein is regulated by its mRNA localization. Our results suggest that the RNA localization mechanism is used not only for restricted accumulation of patterning molecules but also for the microtubule organization that leads to the initial development of oocyte polarity.
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Gwangjin-gu, Seoul, South Korea.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Gwangjin-gu, Seoul, South Korea.
authors.forename: Sujung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Gwangjin-gu, Seoul, South Korea.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Gwangjin-gu, Seoul, South Korea.
authors.forename: Hyeran
authors.initials: H
authors.lastname: Shim
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Gwangjin-gu, Seoul, South Korea.
authors.forename: Jeongsil
authors.initials: J
authors.lastname: Kim-Ha
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D016764:Cell Polarity; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006579:Heterozygote; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009865:Oocytes / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009866:Oogenesis / Q000235:genetics / Q000502:physiology*; D023121:RNA, Messenger, Stored / Q000378:metabolism*
chemical_list: C061998:BicD protein, Drosophila; D029721:Drosophila Proteins; D008840:Microfilament Proteins; D023121:RNA, Messenger, Stored; C450828:shot protein, Drosophila
keywords: Drosophila; mRNA localization; microtubule organizing center; oocyte polarity; short stop; spectraplakin