title: An in depth proteomic analysis based on ProteoMiner, affinity chromatography and nano-HPLC-MS/MS to explain the potential health benefits of bovine colostrum.
abstract: Bovine colostrum (BC), the initial milk secreted by the mammary gland immediately after parturition, is widely used for several health applications. We here propose an off-target method based on proteomic analysis to explain at molecular level the potential health benefits of BC. The method is based on the set-up of an exhaustive protein data bank of bovine colostrum, including the minor protein components, followed by a bioinformatic functional analysis. The proteomic approach based on ProteoMiner technology combined to a highly selective affinity chromatography approach for the immunoglobulins depletion, identified 1786 proteins (medium confidence; 634 when setting high confidence), which were then clustered on the basis of their biological function. Protein networks were then created on the basis of the biological functions or health claims as input. A set of 93 proteins involved in the wound healing process was identified. Such an approach also permits the exploration of novel biological functions of BC by searching in the database the presence of proteins characterized by innovative functions. In conclusion an advanced approach based on an in depth proteomic analysis is reported which permits an explanation of the wound healing effect of bovine colostrum at molecular level and allows the search of novel potential beneficial effects.
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Altomare
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milan, Via Mancinelli 7, 20131 Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Fasoli
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Colzani
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Ximena Maria
authors.initials: XM
authors.lastname: Paredes Parra
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Ferrari
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cilurzo
authors.affiliation: Department of Pharmacology and Biomolecular Sciences, Università degli Studi di Milano, Via Trentacoste 2, 20133 Milan, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Rumio
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy; Systems Biology Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Luca
authors.initials: L
authors.lastname: Cannizzaro
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Carini
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milan, Via Mancinelli 7, 20131 Milan, Italy.
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy. Electronic address: giancarlo.aldini@unimi.it.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Aldini
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003126:Colostrum / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods*; D011247:Pregnancy; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020543:Proteome
keywords: Bovine colostrum proteome; Combinatorial peptide ligand libraries; Low-abundance proteins; Mass spectrometry; Wound healing