title: Cellular transcriptomics: gelsolin negatively regulates the expression of apoptosis-associated genes and inhibits apoptosis in hepatocarcinoma cells.
abstract: Gelsolin (GSN), which is a Ca(2+)-dependent actin filament severing and capping protein, plays a critical role in the cancer progress and has the potential for providing a novel thread for cancer therapy. In current study, we demonstrate the roles of GSN on anti-apoptosis of hepatocarcinoma cells by transcriptome RNA-seq method. Then flow cytometry (FCM), in-cell immunoblotting and transmission electron microscopy (TEM) were used to examine the GSN regulatory cell apoptosis. The results revealed GSN significantly suppresses apoptosis-associated functional categories through down-regulating apoptosis-associated genes in 5 apoptosis terms and 6 relevant KEGG pathways. FCM showed a significant lower apoptotic rate in GSN-SMMC7721 (P<0.05). In-cell immunoblotting detected discrepant expression of the apoptosis factors among GSN expressed/shRNA transfectants (P<0.05). TEM observed the discernible apoptosis morphology. Above results suggest a negative relationship between GSN expression and hepatocarcinoma cell apoptosis. GSN overexpression suppresses apoptosis while down-regulated GSN promotes apoptosis. The possible mechanism could be associated with the regulation of GSN on the apoptosis-associated pathways and the apoptosis factors caspase 3 and bcl-2.
authors.affiliation: Medical Scientific Research Center of Guangxi Medical UniversityNanning 530021, P. R. China; Key Laboratory of High-Incidence-Tumor Prevention & Treatment (Guangxi Medical University), Ministry of EducationNanning 530021, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Public Health, Guangxi Medical University Nanning 530021, P. R. China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Diabetes and Metabolic Diseases, Beckman Research Institute of The City of Hope Duarte, CA 91010, USA.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Medical Scientific Research Center of Guangxi Medical UniversityNanning 530021, P. R. China; Key Laboratory of High-Incidence-Tumor Prevention & Treatment (Guangxi Medical University), Ministry of EducationNanning 530021, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zang
authors.affiliation: Medical Scientific Research Center of Guangxi Medical University Nanning 530021, P. R. China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Li
authors.affiliation: Medical Scientific Research Center of Guangxi Medical University Nanning 530021, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Medical Scientific Research Center of Guangxi Medical UniversityNanning 530021, P. R. China; Key Laboratory of High-Incidence-Tumor Prevention & Treatment (Guangxi Medical University), Ministry of EducationNanning 530021, P. R. China.
authors.forename: Danrong
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Public Health, Guangxi Medical University Nanning 530021, P. R. China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Diabetes and Metabolic Diseases, Beckman Research Institute of The City of Hope Duarte, CA 91010, USA.
authors.forename: Wendong
authors.initials: W
authors.lastname: Huang
authors.affiliation: School of Public Health, Guangxi Medical UniversityNanning 530021, P. R. China; Key Laboratory of High-Incidence-Tumor Prevention & Treatment (Guangxi Medical University), Ministry of EducationNanning 530021, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D045744:Cell Line, Tumor; D018260:Gelsolin / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D034622:RNA Interference; D015398:Signal Transduction; D014162:Transfection
chemical_list: D051017:Apoptosis Regulatory Proteins; D018260:Gelsolin
keywords: Hepatocarcinoma; RNA-seq; anti-apoptosis; gelsolin; transcriptomics
references.citation: FEBS Lett. 1997 Apr 7;406(1-2):49-55
references.pmid: 9109384
references.citation: Urology. 2003 Apr;61(4):845-50
references.pmid: 12670587
references.citation: Cancer. 1999 Jan 1;85(1):47-57
references.pmid: 9921973
references.citation: Prostate. 1999 Jun 15;40(1):14-9
references.pmid: 10344719
references.citation: Eur J Cancer. 2005 Feb;41(3):461-9
references.pmid: 15691647
references.citation: Oral Oncol. 2006 Jul;42(6):599-606
references.pmid: 16753328
references.citation: Endocrinology. 2007 Mar;148(3):1306-12
references.pmid: 17170101
references.citation: Trends Genet. 2008 Mar;24(3):133-41
references.pmid: 18262675
references.citation: Science. 2008 Jun 6;320(5881):1344-9
references.pmid: 18451266
references.citation: Nat Methods. 2008 Jul;5(7):621-8
references.pmid: 18516045
references.citation: Science. 2008 Aug 15;321(5891):956-60
references.pmid: 18599741
references.citation: Hum Pathol. 2008 Oct;39(10):1420-30
references.pmid: 18656242
references.citation: Mol Med. 2008 Sep-Oct;14(9-10):582-9
references.pmid: 18584046
references.citation: Nat Rev Genet. 2009 Jan;10(1):57-63
references.pmid: 19015660
references.citation: Nature. 2009 Mar 5;458(7234):97-101
references.pmid: 19136943
references.citation: N Engl J Med. 2009 Jun 25;360(26):2719-29
references.pmid: 19516027
references.citation: Nat Rev Genet. 2010 Jan;11(1):31-46
references.pmid: 19997069
references.citation: J Neurosci Methods. 2010 Nov 30;193(2):189-202
references.pmid: 20800617
references.citation: Genome Res. 2011 Jan;21(1):56-67
references.pmid: 21036922
references.citation: Nat Rev Genet. 2011 Feb;12(2):87-98
references.pmid: 21191423
references.citation: Biochem Biophys Res Commun. 2011 Aug 19;412(1):44-9
references.pmid: 21801713
references.citation: Annu Rev Genomics Hum Genet. 2011;12:407-30
references.pmid: 21639794
references.citation: PLoS One. 2011;6(10):e26168
references.pmid: 22043308
references.citation: Adv Exp Med Biol. 2012;723:519-25
references.pmid: 22183372
references.citation: Cancer. 2002 Sep 15;95(6):1247-57
references.pmid: 12216092
references.citation: Ann Surg. 2015 Jan;261(1):56-66
references.pmid: 25072444
references.citation: Int J Mol Med. 2003 Sep;12(3):341-7
references.pmid: 12883650
references.citation: Biochem Biophys Res Commun. 2003 Dec 26;312(4):1335-41
references.pmid: 14652020
references.citation: J Gastroenterol Hepatol. 2004 Feb;19(2):218-24
references.pmid: 14731134
references.citation: Nat Rev Cancer. 2004 Apr;4(4):253-65
references.pmid: 15057285
references.citation: J Biol Chem. 1988 Sep 25;263(27):13857-62
references.pmid: 2843540
references.citation: J Biol Chem. 2000 Feb 11;275(6):3761-6
references.pmid: 10660524
references.citation: Oncogene. 2000 Oct 5;19(42):4807-14
references.pmid: 11039896
references.citation: EMBO J. 2000 Oct 2;19(19):5123-34
references.pmid: 11013215
references.citation: Breast Cancer Res Treat. 2001 Jan;65(1):11-21
references.pmid: 11245335
references.citation: Clin Cancer Res. 2001 Aug;7(8):2415-24
references.pmid: 11489821
references.citation: Am J Pathol. 1989 Mar;134(3):597-603
references.pmid: 2538057
references.citation: Eur J Biochem. 1990 Jul 5;190(3):559-65
references.pmid: 2164930
references.citation: Oncogene. 1993 Sep;8(9):2531-6
references.pmid: 8395682
references.citation: Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5682-5
references.pmid: 8202548
references.citation: Cancer Res. 1995 Aug 1;55(15):3228-32
references.pmid: 7614452
references.citation: Exp Cell Res. 1995 Aug;219(2):384-91
references.pmid: 7641789
references.citation: Genome Res. 2012 Feb;22(2):299-306
references.pmid: 22009989
references.citation: Cell Res. 2012 May;22(5):806-21
references.pmid: 22349460
references.citation: PLoS One. 2012;7(5):e37159
references.pmid: 22606345
references.citation: Med Res Rev. 2012 Sep;32(5):999-1025
references.pmid: 22886630
references.citation: J Proteomics. 2013 Jan 14;78:362-73
references.pmid: 23085225
references.citation: BMC Genomics. 2013;14:543
references.pmid: 23937650
references.citation: Neoplasma. 2014;61(1):17-26
references.pmid: 24195504
references.citation: Anticancer Res. 2014 Aug;34(8):4399-410
references.pmid: 25075077
references.citation: Cancer Res. 1998 Jan 15;58(2):322-7
references.pmid: 9443412