title: Decreased Expression of Caveolin-1 and E-Cadherin Correlates with the Clinicopathologic Features of Gastric Cancer and the EMT Process.
abstract: BACKGROUND
  Recent researches and relevant patents have been reported to prove the significant value of Caveolin-1 (Cav-1) in cancer diagnosis and treatment.
  
  
  OBJECTIVE
  Our study aimed to study the role of Cav-1 in gastric cancer progression and investigate the relationship between Cav-1/E-cadherin expression and the clinical status of gastric cancer.
  
  
  METHODS
  Immunohistochemistry was applied to detect the expression of Cav-1 and E-cadherin in gastric cancer in a tissue microarray. Real-time PCR was used to further detect the mRNA expression of Cav-1 and E-cadherin in tumor-derived and peritumoral tissues and in different gastric cancer cell lines. The expression of E-cadherin was analyzed by Western Blot and the cell migration ability was examined by Transwell migration assays after downregulation of Cav-1 using siRNA.
  
  
  RESULTS
  The staining of Cav-1 and E-cadherin were both strong in all 5 of the normal gastric tissues, while in gastric cancer tissues the staining of Cav-1 and E-cadherin were downregulated (44&33 negative, 21&22weak and 5&15 strong). And their level was correlated with tumor clinical stage, pathological grade, and metastasis status. Moreover, there was a positive correlation between Cav-1 expression and E-cadherin expression in gastric cancer tissues(r = 0.42, P < 0.05). Knockdown of Cav-1 resulted in decreased expression of E-cadherin, cell morphology changes and elevated migration ability of gastric cancer cells.
  
  
  CONCLUSIONS
  Decreased expression of Cav-1 and E-cadherin may play an important role in the progression of gastric cancer; Knockdown of Cav-1 may promote EMT of gastric cancer by targeting E-cadherin.
authors.forename: Kundong
authors.initials: K
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Weidong
authors.initials: W
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Xia
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Kejan
authors.initials: K
authors.lastname: Huang
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, Shanghai Jiaotong University Affiliated First Peoples hospital, 100 Haining Road, Shanghai 200080, P.R China. richard-hc@hotmail.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D051242:Caveolin 1 / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016037:Single-Blind Method; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C495540:CAV1 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D051242:Caveolin 1