title: Biophysical properties of dermal building-blocks affects extra cellular matrix assembly in 3D endogenous macrotissue.
abstract: The fabrication of functional tissue units is one of the major challenges in tissue engineering due to their in vitro use in tissue-on-chip systems, as well as in modular tissue engineering for the construction of macrotissue analogs. In this work, we aim to engineer dermal tissue micromodules obtained by culturing human dermal fibroblasts into porous gelatine microscaffold. We proved that such stromal cells coupled with gelatine microscaffolds are able to synthesize and to assemble an endogenous extracellular matrix (ECM) resulting in tissue micromodules, which evolve their biophysical features over the time. In particular, we found a time-dependent variation of oxygen consumption kinetic parameters, of newly formed ECM stiffness and of micromodules self-aggregation properties. As consequence when used as building blocks to fabricate larger tissues, the initial tissue micromodules state strongly affects the ECM organization and maturation in the final macrotissue. Such results highlight the role of the micromodules properties in controlling the formation of three-dimensional macrotissue in vitro, defining an innovative design criterion for selecting tissue-building blocks for modular tissue engineering.
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, I-80125 Napoli, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Urciuolo
authors.forename: A
authors.initials: A
authors.lastname: Garziano
authors.forename: G
authors.initials: G
authors.lastname: Imparato
authors.forename: V
authors.initials: V
authors.lastname: Panzetta
authors.forename: S
authors.initials: S
authors.lastname: Fusco
authors.forename: C
authors.initials: C
authors.lastname: Casale
authors.forename: P A
authors.initials: PA
authors.lastname: Netti
mesh_terms: D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000502:physiology* / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008904:Miniaturization; D009924:Organ Culture Techniques / Q000295:instrumentation; D066330:Printing, Three-Dimensional; D012867:Skin / Q000166:cytology / Q000254:growth & development*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds