title: Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium.
abstract: Non-equilibrium atmospheric pressure plasmas generate a high electron density (on the order of 10(16) electrons per cm(-3)) using Ar gas. Culture medium in air at room temperature was plasma-irradiated for several hundred seconds. Tens of micromolar hydrogen peroxide (H2O2) and millimolar levels of nitrous ion (NO2(-)) were detected in the plasma-irradiated culture medium (plasma activated medium; PAM) and selectively induced the apoptotic death of glioblastoma tumor cells, but did not kill normal mammary epithelial cells. A similar antitumor effect was induced by spiking the medium with comparable concentrations of H2O2 and NO2(-). The PAM remained still a somewhat difference that it should also be assessed for understanding other latent mechanisms.
authors.affiliation: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8603, Japan. Electronic address: kurake.naoyuki@f.mbox.nagoya-u.ac.jp.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kurake
authors.affiliation: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8603, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8603, Japan. Electronic address: ishikawa.kenji@nagoya-u.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: Toyama University, 2630 Sugitani, Toyama, 930-0194, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8603, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sekine
authors.affiliation: Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa, Nagoya, 466-8560, Japan.
authors.forename: Kae
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa, Nagoya, 466-8560, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kajiyama
authors.affiliation: Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa, Nagoya, 466-8560, Japan.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kikkawa
authors.affiliation: Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa, Nagoya, 466-8560, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Mizuno
authors.affiliation: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8603, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Hori
mesh_terms: D000818:Animals; D017209:Apoptosis; D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry*; D004583:Electrons; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D009566:Nitrates / Q000737:chemistry; D009573:Nitrites / Q000737:chemistry*; D009585:Nitrogen Dioxide / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D058626:Plasma Gases; D026361:Reactive Nitrogen Species / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D003470:Culture Media; D009566:Nitrates; D009573:Nitrites; D016877:Oxidants; D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D009585:Nitrogen Dioxide
keywords: Apoptosis; Glioblastoma; Hydrogen peroxide; Nitrites; Non-equilibrium atmospheric pressure plasma; Plasma activated medium