title: Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages.
abstract: A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5'-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.|State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Haimin
authors.initials: H
authors.lastname: Feng
authors.affiliation: Hubei Center for Disease Control and Prevention, Wuhan, 430079, Hubei, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Da
authors.affiliation: Hubei Center for Disease Control and Prevention, Wuhan, 430079, Hubei, People's Republic of China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Lang
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Xie
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.|State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Huang
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.|State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Xiong
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China.|State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Kang
authors.affiliation: Department of Zoology, College of Life Sciences, Nanjing Agriculture University, Nanjing, 210095, Jiangsu, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Microbiology and Immunology, Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX, 77555, USA.
authors.forename: Haitao
authors.initials: H
authors.lastname: Hu
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China. houwei@whu.edu.cn.|State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China. houwei@whu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuchang, Wuhan, 430070, Hubei, People's Republic of China. yongfeng@whu.edu.cn.|State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China. yongfeng@whu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Feng
mesh_terms: D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D016325:HIV Long Terminal Repeat / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000821:virology*; D035683:MicroRNAs / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication / Q000502:physiology*
chemical_list: D035683:MicroRNAs