title: Mcy protein, a potential antidiabetic agent: evaluation of carbohydrate metabolic enzymes and antioxidant status.
abstract: The objective of the present study is to elucidate the long-term effects of anti-hyperglycemic active principle, Mcy protein (MCP), isolated from the fruits of Momordica cymbalaria on carbohydrate metabolism and oxidative stress in experimental diabetic rats. We used streptozotocin induced diabetic rats for the current studies. Our studies showed that MCP (2.5mg/kg.b.w) treatment significantly normalized the deranged activities of critical carbohydrate metabolizing enzymes, hexokinase, glucose-6-phosphate dehydrogenase, glucose-6-phosphatase and fructose-1,6-bis phosphatase. In addition MCP showed inhibitory activity on α-glucosidase and aldose reductase enzymes in in vitro assays. Further MCP treatment improved the antioxidant defensive mechanism by preventing deleterious oxidative products of cellular metabolism, which initiates the lipid peroxidation and by normalizing the antioxidant enzyme (catalase, superoxide dismutase, glutathione peroxidase) activities. Additional structural studies using circular dichroism spectroscopy indicate that MCP contains majorly α-helix. Our findings suggest MCP regulates blood glucose and better manage diabetes mellitus associated complications by regulating carbohydrate metabolism and by protecting from the deleterious effects of oxidative stress.
authors.affiliation: Department of Biochemistry, S.V. University, Tirupati 517502, India.
authors.forename: Saritha
authors.initials: S
authors.lastname: Marella
authors.affiliation: Department of Biochemistry, S.V. University, Tirupati 517502, India.
authors.forename: Dilip Rajasekhar
authors.initials: DR
authors.lastname: Maddirela
authors.affiliation: Department of Biochemistry, S.V. University, Tirupati 517502, India; Department of Endocrinology, SVIMS, Tirupati 517502, India.
authors.forename: E G T V
authors.initials: EG
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, S.V. University, Tirupati 517502, India.
authors.forename: Thandaiah Krishna
authors.initials: TK
authors.lastname: Tilak
authors.affiliation: Department of Biology, Savannah State University, Savannah, GA, USA. Electronic address: badrik@savannahstate.edu.
authors.forename: Kameswara Rao
authors.initials: KR
authors.lastname: Badri
authors.affiliation: Department of Biochemistry, S.V. University, Tirupati 517502, India. Electronic address: chippadar@yahoo.com.
authors.forename: Apparao
authors.initials: A
authors.lastname: Chippada
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism; D005215:Fasting / Q000097:blood; D006003:Glycogen / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D029775:Momordica / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010940:Plant Proteins; D006003:Glycogen; D000449:Aldehyde Reductase; D000520:alpha-Glucosidases
keywords: Carbohydrate metabolism enzymes; Diabetes mellitus; Lipid peroxidation; Mcy protein; Momordica cymbalaria; Streptozotocin