title: Coronary leukocyte activation in relation to progression of coronary artery disease.
abstract: Leukocyte activation has been linked to atherogenesis, but there is little in vivo evidence for its role in the progression of atherosclerosis. We evaluated the predictive value for progression of coronary artery disease (CAD) of leukocyte activation markers in the coronary circulation. Monocyte and neutrophil CD11b, neutrophil CD66b expression and intracellular neutrophil myeloperoxidase (MPO) in the coronary arteries were determined by flow cytometry in patients undergoing coronary angiography. The primary outcome included fatal and nonfatal myocardial infarction or arterial vascular intervention due to unstable angina pectoris. In total 99 subjects who were included, 70 had CAD at inclusion (26 patients had single-vessel disease, 18 patients had twovessel disease and 26 patients had three-vessel disease). The median follow-up duration was 2242 days (interquartile range: 2142-2358). During follow-up, 13 patients (13%) developed progression of CAD. Monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO measured in blood obtained from the coronary arteries were not associated with the progression of CAD. These data indicate that coronary monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO do not predict the risk of progression of CAD.
authors.affiliation: Department of Internal Medicine, Centre for Diabetes and Vascular Medicine, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands. m.devries@franciscus.nl.
authors.forename: Marijke A
authors.initials: MA
authors.lastname: de Vries
authors.affiliation: Department of Internal Medicine, Centre for Diabetes and Vascular Medicine, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Arash
authors.initials: A
authors.lastname: Alipour
authors.affiliation: Department of Statistics and Education, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Erwin
authors.initials: E
authors.lastname: Birnie
authors.affiliation: Department of Internal Medicine, Centre for Diabetes and Vascular Medicine, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Andrew
authors.initials: A
authors.lastname: Westzaan
authors.affiliation: Department of Internal Medicine, Centre for Diabetes and Vascular Medicine, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Selvetta
authors.initials: S
authors.lastname: van Santen
authors.affiliation: Department of Clinical Chemistry, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Ellen
authors.initials: E
authors.lastname: van der Zwan
authors.affiliation: Department of Cardiology, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Anho H
authors.initials: AH
authors.lastname: Liem
authors.affiliation: Department of Internal Medicine, Centre for Diabetes and Vascular Medicine, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Noëlle
authors.initials: N
authors.lastname: van der Meulen
authors.affiliation: Department of Internal Medicine, Centre for Diabetes and Vascular Medicine, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands.
authors.forename: Manuel Castro
authors.initials: MC
authors.lastname: Cabezas
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D039481:CD11b Antigen / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D003324:Coronary Artery Disease / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000032:analysis; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; C509295:ITGAM protein, human; D009195:Peroxidase
keywords: coronary artery disease; inflammation; integrin; leukocyte activa; myeloperoxidase
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