title: Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology.
abstract: Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values (98.61 ± 0.61).
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; College of Traditional Chinese Medicine, Yunnan College of Traditional Chinese Medicine, Kunming 650500, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Chu
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tian
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; College of Traditional Chinese Medicine, Yunnan College of Traditional Chinese Medicine, Kunming 650500, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Wanyi
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Yuanzhong
authors.identifier: 0000-0001-5376-757X
authors.initials: Y
authors.lastname: Wang
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