title: Irradiation with narrowband-ultraviolet B suppresses phorbol ester-induced up-regulation of H1 receptor mRNA in HeLa cells.
abstract: Conclusion These findings suggest that low dose irradiation with 310 nm NB-UVB specifically suppressed the up-regulation of H1R gene expression without inducing apoptosis and that UVB of shorter or longer wavelength than 310 nm NB-UVB had no such effects. Objective To develop a narrowband-ultraviolet B(NB-UVB) phototherapy for allergic rhinitis, this study investigated the effects of irradiation with NB-UVB at wavelength of 310 nm on phorbol-12-myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) mRNA in HeLa cells. Methods The mRNA levels of H1R in HeLa cells were measured using real-time RT-PCR. Apoptosis were evaluated with DNA fragmentation assay. Results PMA induced a significant increase in H1R mRNA expression in HeLa cells. Irradiation with 305 nm UVB and 310 nm NB-UVB, but not with 315 nm UVB at doses of 200 and 300 mJ/cm(2) significantly suppressed PMA-induced up-regulation of H1R mRNA. At a dose of 200 mJ/cm(2), irradiation with 305 nm UVB, but not with 310 nm NB-UVB, induced apoptosis, although exposure of the cells to both 305 and 310 nm UVB induced apoptosis at a dose of 300 mJ/cm(2) after PMA treatment in HeLa cells. Conversely, irradiation with 315 nm UVB at doses of 200 and 300 mJ/cm(2) did not induce apoptosis.
authors.affiliation: a Department of Otolaryngology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kitamura
authors.affiliation: b Department of Molecular Pharmacology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.affiliation: b Department of Molecular Pharmacology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Kentaro
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: b Department of Molecular Pharmacology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Mika
authors.initials: M
authors.lastname: Kitayama
authors.affiliation: a Department of Otolaryngology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujii
authors.affiliation: c Nichia Corporation , Tokushima , Japan ;
authors.forename: Akira
authors.initials: A
authors.lastname: Fujioka
authors.affiliation: c Nichia Corporation , Tokushima , Japan ;
authors.forename: Toshio
authors.initials: T
authors.lastname: Matsushita
authors.affiliation: c Nichia Corporation , Tokushima , Japan ;
authors.forename: Takashi
authors.initials: T
authors.lastname: Mukai
authors.affiliation: d Department of Dermatology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kubo
authors.affiliation: e Miki Medical Corporation SunField Stem Cell Processing Center , Tokyo , Japan ;
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kubo
authors.affiliation: f Department of Molecular Studies for Incurable Diseases , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukui
authors.affiliation: a Department of Otolaryngology , Institute of Medical Biosciences, Tokushima University Graduate School , Tokushima , Japan ;
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takeda
mesh_terms: D004847:Epithelial Cells / Q000378:metabolism / Q000528:radiation effects*; D006367:HeLa Cells; D006801:Humans; D010703:Phorbol Esters; D011969:Receptors, Histamine H1 / Q000378:metabolism*; D065631:Rhinitis, Allergic / Q000532:radiotherapy*; D014467:Ultraviolet Therapy
chemical_list: D010703:Phorbol Esters; D011969:Receptors, Histamine H1
keywords: Histamine H1 receptor; allergic rhinitis; irradiation; narrowband-ultraviolet B; phototherapy