title: Wound healing and antimicrobial activity of two classical formulations of Laghupanchamula in rats.
abstract: BACKGROUND
  Wounds affect a large number of patients and seriously reduce the quality of life. The wound as a medical problem was first discussed by Maharshi Agnivesha in Agnivesha Samhita (later known as Charaka Samhita) as Vrana. Laghupanchamula denotes a combination of the roots of five herbs. However, in Ayurvedic classics, besides four common herbs viz. Kantakari, Brihati, Shalaparni and Prinshniparni, the fifth one is either Gokshura (LPG) or Eranda (LPE), and both formulations have been documented to have wound healing (Vrana) activity.
  
  
  OBJECTIVE
  The present study was undertaken to determine the in vivo wound healing activity and in vitro antimicrobial activity of 50% ethanolic extract of Laghupanchamula containing Gokshura (LPGE) and Laghupanchamula containing Eranda (LPEE) in rats with acute toxicity in mice.
  
  
  METHODS
  LPGE and LPEE (1000 mg/kg) was administered orally, once daily for 10 days (incision wound model) or for 24 days (excision wound model) in rats. LPGE and LPEE was studied for its in vitro antimicrobial and in vivo wound breaking strength (WBS) (incision model) and rate of contraction, period of epithelization and histology of skin (excision model).
  
  
  CONCLUSIONS
  LPGE and LPEE showed antimicrobial activity against skin pathogens, enhanced WBS, rate of contraction, skin collagen tissue formation and early epithelization period with low scar area indicating enhanced healing with histological evidence of more collagen formation in skin tissues. LPGE and LPEE also showed anti-bacterial activity and seemed to be safe, and use of both formulations in Laghupanchamula for their wound healing and anti-microbial activities is thus authenticated.
authors.affiliation: Department of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Shivani
authors.initials: S
authors.lastname: Ghildiyal
authors.affiliation: Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Manish K
authors.initials: MK
authors.lastname: Gautam
authors.affiliation: Department of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Joshi
authors.affiliation: Department of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Raj K
authors.initials: RK
authors.lastname: Goel
keywords: Antimicrobial; Laghupanchamula; excision wound; incision wound; minimum inhibitory concentration
references.citation: Fitoterapia. 2010 Sep;81(6):503-8
references.pmid: 20083169
references.citation: Pharmacogn Rev. 2011 Jul;5(10):120-37
references.pmid: 22279370
references.citation: J Toxicol. 2014;2014:135654
references.pmid: 24616736
references.citation: J Ayurveda Integr Med. 2013 Jan;4(1):23-7
references.pmid: 23741158
references.citation: J Cell Commun Signal. 2009 Dec;3(3-4):287-310
references.pmid: 19838819
references.citation: Biomed Res Int. 2013;2013:972028
references.pmid: 23984424
references.citation: Clin Microbiol Rev. 2001 Apr;14(2):244-69
references.pmid: 11292638
references.citation: Nat Protoc. 2008;3(2):163-75
references.pmid: 18274517
references.citation: Phytochemistry. 2007 Jun;68(12):1692-7
references.pmid: 17540419
references.citation: J Ethnopharmacol. 2010 May 4;129(1):106-14
references.pmid: 20132876
references.citation: Toxicol Mech Methods. 2011 Mar;21(3):246-50
references.pmid: 21142779
references.citation: Yakugaku Zasshi. 1996 Mar;116(3):238-43
references.pmid: 8721352
references.citation: BMC Complement Altern Med. 2011 Oct 07;11:86
references.pmid: 21982053
references.citation: J Ethnopharmacol. 2009 Jul 15;124(2):311-5
references.pmid: 19397984
references.citation: Wound Repair Regen. 2003 Mar;11 Suppl 1:S1-28
references.pmid: 12654015
references.citation: Int J Low Extrem Wounds. 2003 Mar;2(1):25-39
references.pmid: 15866825
references.citation: Anc Sci Life. 2007 Jan;26(3):45-51
references.pmid: 22557241
references.citation: ScientificWorldJournal. 2014 Mar 04;2014:740107
references.pmid: 24737990
references.citation: J Cell Physiol. 2002 Nov;193(2):173-9
references.pmid: 12384994
references.citation: Lett Appl Microbiol. 2012 May;54(5):475-82
references.pmid: 22394164