title: Germinating Peanut (Arachis hypogaea L.) Seedlings Attenuated Selenite-Induced Toxicity by Activating the Antioxidant Enzymes and Mediating the Ascorbate-Glutathione Cycle.
abstract: Selenite can enhance the selenium nutrition level of crops, but excessive selenite may be toxic to plant growth. To elucidate the mechanisms underlying the role of selenite in production and detoxification of oxidative toxicity, peanut seedlings were developed with sodium selenite (0, 3, and 6 mg/L). The effects of selenite on antioxidant capacity, transcript levels of antioxidant enzyme genes, and enzyme activities in hypocotyl were investigated. The CuZn-SOD, GSH-Px, GST, and APX gene expression levels and their enzyme activities in selenite treatments were 1.0-3.6-fold of the control. Selenite also significantly increased the glutathione and ascorbate concentrations by mediating the ascorbate-glutathione cycle, and the selenite-induced hydrogen peroxide may act as a second messenger in the signaling pathways. This work has revealed a complex antioxidative response to selenite in peanut seedling. Understanding these mechanisms may help future research in increasing selenite tolerance and selenium accumulation in peanut and other crops.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou 510640, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou 510640, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou 510640, China.
authors.forename: Furao
authors.initials: F
authors.lastname: Lai
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou 510640, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D010367:Arachis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D018525:Germination / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D020887:Selenious Acid / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D020887:Selenious Acid; D005978:Glutathione; D001205:Ascorbic Acid
keywords: antioxidant enzyme; ascorbate−glutathione cycle; gene expression; peanut; selenite; selenium