title: In vitro antimicrobial activities of different solvent extracted samples from Iris germinica.
abstract: Antibacterial and antifungal activities of different solvents extracted samples of Iris germinica were carried out through disc diffusion assay. For this purpose five different solvent extracts were prepared with two concentrations (1 and 2 mg disc(-1)) and their antimicrobial activity was tested using disc diffusion assay against eight pathogenic bacteria viz. Staphylococcus aureus, B. subtilis, Bacillus atrophaeus, Escherichia coli, Pseudomonas aeruginosa, Erwinia carotovara, Klebsiella pneumoniae, Salmonella typhi and one fungal specie (Candida albicans). Butanol and ethyl acetate fraction were more effective to control the growth of different pathogens followed by chloroform, hexane and aqueous fractions respectively. C. albicans, S. aureus E. carotovara, B. atrophaeus and E. coli were comparatively susceptible inhibited by all extracts of I. germinica compared with the rest of microbes. Maximum activity was shown by ethyl acetate extracted samples against B. atrophaeus followed by the same solvent against E. carotovara. Butanol extracted samples were effective against B. subtilis showing 62% reduction in growth at 1 or 2mg disc(-1) concentration. From these results it can be concluded that different solvent extracted samples from the leaves of I. germinica possess varying degree of antimicrobial against different micro-organisms and can be a good sources of antibiotics for the treatment of certain bacterial and fungal diseases.
authors.affiliation: Department of Botany, Islamia College Peshawar, KPK, Pakistan.
authors.forename: Amjed
authors.initials: A
authors.lastname: Uzair
authors.affiliation: Institute of Biotechnology & Genetic Engineering, The University of Agriculture, Peshawar, KPK Pakistan.
authors.forename: Jehan
authors.initials: J
authors.lastname: Bakht
authors.affiliation: Department of Botany, Islamia College Peshawar, KPK, Pakistan.
authors.forename: Arshad
authors.initials: A
authors.lastname: Iqbal
authors.affiliation: Department of Agriculture, University of Haripur, KPK, Pakistan.
authors.forename: Khalid
authors.initials: K
authors.lastname: Naveed
authors.affiliation: Department of Agriculture, University of Haripur, KPK, Pakistan.
authors.forename: Naushad
authors.initials: N
authors.lastname: Ali
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D007498:Iris; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts