title: An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens.
abstract: The ability to take targeted multiple cores from a single frozen biospecimen would enable several research projects to be fueled from one biospecimen, a small piece of tissue to be quality-control tested, and for pathologically-discrete areas of a biospecimen (e.g., tumor, stromal, and normal tissue) to be selectively sampled for comparative analyses. CryoXtract Instruments' CXT350 Frozen Sample Aliquotter can potentially achieve this by producing multiple cores from one cryopreserved biospecimen without thawing either the parent biospecimen or its daughter cores. It therefore has the potential to add significant value to a tissue banking workflow. We have evaluated its performance while using 614 cores from fecal, liver, kidney, lung, heart, and colon biospecimens. Coring densities of up to five complete and four fragmentary cores per cm(3) are achievable using 3 mm coring probes. Median core weights for tissue were 14.1-17.2 mg (depending on tissue type) and cores ≤325 mg could be taken from fecal biospecimens (depending on the fill-depth of the tube). The coefficient of variation for multiple cores taken from a fecal biospecimen was 11.7%. Between-sample contamination did not occur. RNA Integrity numbers and qRT-PCR analysis demonstrated that coring induced a statistically significant impact on RNA quality that was inconsequential in magnitude and in our view does not represent a barrier for the effective utilization of the technology.
authors.affiliation: Integrated Biobank of Luxembourg , Luxembourg .
authors.forename: William
authors.initials: W
authors.lastname: Mathieson
authors.affiliation: Integrated Biobank of Luxembourg , Luxembourg .
authors.forename: Ignacio
authors.initials: I
authors.lastname: Sanchez
authors.affiliation: Integrated Biobank of Luxembourg , Luxembourg .
authors.forename: Kathleen
authors.initials: K
authors.lastname: Mommaerts
authors.affiliation: Integrated Biobank of Luxembourg , Luxembourg .
authors.forename: Sonia
authors.initials: S
authors.lastname: Frasquilho
authors.affiliation: Integrated Biobank of Luxembourg , Luxembourg .
authors.forename: Fay
authors.initials: F
authors.lastname: Betsou
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D011786:Quality Control; D013048:Specimen Handling / Q000379:methods; D014015:Tissue Banks