title: MicroRNA-7 downregulates the oncogene VDAC1 to influence hepatocellular carcinoma proliferation and metastasis.
abstract: Recent studies have been shown that voltage-dependent anion channel 1 (VDAC1) plays an important role in carcinogenesis. However, its molecular biological function in hepatocellular carcinoma (HCC) has not been entirely clarified. This study investigated the expression of VDAC1 in HCC and its prognostic value for HCC patients. Furthermore, we also identify the relevant VDAC1 direct target. Western blot, real-time quantitative PCR (qRT-PCR), and immunohistochemical (IHC) staining were performed to detect the expression of VDAC1 in HCC. Furthermore, the relationship between the VDAC1 level and clinicopathological features and prognostic values was explored. The effects of VDAC1 on HCC cell proliferation, migration, and invasion were also investigated in vitro. Predicted target gene of VDAC1 was determined by dual-luciferase reporter assay, qRT-PCR, and Western blot analyses. Our results revealed elevated VDAC1 messenger RNA (mRNA) (P = 0.0020) and protein (P = 0.0035) expression in tumor tissue samples compared with paired adjacent non-tumorous tissue samples. High VDAC1 expression was correlated with distant metastasis (P = 0.025), differentiation (P = 0.002), and advanced tumor stage (P = 0.004) in HCC patients. Kaplan-Meier survival analysis demonstrated that high expression of VDAC1 was significantly correlated with a poor prognosis for HCC patients (P < 0.001). The multivariate analysis revealed that VDAC1 expression was an independent prognostic factor of the overall survival rate of HCC patients. Furthermore, knockdown of VDAC1 inhibits HCC cell proliferation, migration, and invasion in vitro. Moreover, further study revealed that miR-7 was a putative target of VDAC1. Our study suggested that miR-7 suppressed the expression of VDAC1. VDAC1 plays an important role in tumor progression and may be used as a potential role in the prognosis of HCC patients.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Feiran
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The First People's Hospital of Jingmen, Dongbao District, Jingmen, Hubei, 448000, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qiang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Yasu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Yinda
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Xian
authors.initials: X
authors.lastname: Shao
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, People's Republic of China. chen316nt@sina.com.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Xi Si Road, Nantong, Jiangsu Province, 226001, People's Republic of China. Chenzgs@163.com.|Department of Hepatobiliary Surgery, Affiliated Hospital of Nantong University and Research Institute of Hepatobiliary Surgery of Nantong University, 20 Xisi Road, Nantong, Jiangsu, 226001, People's Republic of China. Chenzgs@163.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D050995:Voltage-Dependent Anion Channel 1 / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D050995:Voltage-Dependent Anion Channel 1; C549242:MIRN7-1 microRNA, human; C494762:VDAC1 protein, human
keywords: Hepatocellular carcinoma; MiR-7; Prognosis; VDAC1
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