title: Selective sensitization of C-fiber nociceptors by hydrogen sulfide.
abstract: We examined the effects of intraplantar (i.pl.) administration of NaHS, an H2S donor, known to cause T-type Ca(2+) channel (T-channel)-dependent mechanical hyperalgesia, on responsiveness to electric stimulation with 5, 250 and 2000 Hz sine waves (SW) that selectively excites C, Aδ and Aβ fibers, respectively. NaHS, given i.pl., caused behavioral hypersensitivity to SW stimulation at 5 Hz, but not 250 or 2000 Hz, in rats. NaHS also enhanced phosphorylation of spinal ERK following 5 Hz SW stimulation. Three distinct T-channel blockers abolished the NaHS-induced behavioral hypersensitivity to 5 Hz SW stimulation. Thus, H2S selectively sensitizes C-fiber nociceptors via T-channels.
authors.affiliation: Laboratory of Pharmacology & Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Aoki
authors.affiliation: Laboratory of Pharmacology & Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Tsubota
authors.affiliation: Laboratory of Pharmacology & Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Nishimoto
authors.affiliation: Laboratory of Pharmacology & Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Maeda
authors.affiliation: Laboratory of Pharmacology & Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Sekiguchi
authors.affiliation: Laboratory of Pharmacology & Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan. Electronic address: kawabata@phar.kindai.ac.jp.
authors.forename: Atsufumi
authors.initials: A
authors.lastname: Kawabata
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology* / Q000627:therapeutic use; D020747:Calcium Channels, T-Type / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology*; D008297:Male; D009619:Nociceptors / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D017208:Rats, Wistar; D013116:Spinal Cord / Q000201:enzymology; D013440:Sulfides / Q000008:administration & dosage / Q000009:adverse effects
chemical_list: D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type; D013440:Sulfides; D048049:Extracellular Signal-Regulated MAP Kinases; C025451:sodium bisulfide; D006862:Hydrogen Sulfide
keywords: C-fiber nociceptor; Hydrogen sulfide; T-type calcium channel