title: Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification.
abstract: Malaria remains one of the most prevalent infectious diseases and results in significant mortality. Isothermal amplification (loop-mediated isothermal amplification) is used to detect malarial DNA at levels of ~1 parasite/µL blood in ≤30 minutes without the isolation of parasite nucleic acid from subject's blood or saliva. The technique targets the mitochondrial cytochrome oxidase subunit 1 gene and is capable of distinguishing Plasmodium falciparum from Plasmodium vivax. Malarial diagnosis by the gold standard microscopic examination of blood smears is generally carried out only after moderate-to-severe symptoms appear. Rapid diagnostic antigen tests are available but generally require infection levels in the range of 200-2,000 parasites/µL for a positive diagnosis and cannot distinguish if the disease has been cleared due to the persistence of circulating antigen. This study describes a rapid and simple molecular assay to detect malarial genes directly from whole blood or saliva without DNA isolation.
authors.affiliation: Department of Basic Science, New York University College of Dentistry, New York, NY, USA.
authors.forename: Sayli S
authors.initials: SS
authors.lastname: Modak
authors.affiliation: Department of Basic Science, New York University College of Dentistry, New York, NY, USA.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Barber
authors.affiliation: Department of Basic Science, New York University College of Dentistry, New York, NY, USA.
authors.forename: Eran
authors.initials: E
authors.lastname: Geva
authors.affiliation: Department of Basic Science, New York University College of Dentistry, New York, NY, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Abrams
authors.affiliation: Department of Basic Science, New York University College of Dentistry, New York, NY, USA.; Department of Medicine, New York University Langone School of Medicine, New York, NY, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Malamud
authors.affiliation: Department of Basic Science, New York University College of Dentistry, New York, NY, USA.
authors.forename: Yhombi Serge Yvon
authors.initials: YS
authors.lastname: Ongagna
keywords: Plasmodium falciparum; Plasmodium vivax; loop-mediated isothermal amplification (LAMP); malaria; molecular detection; point-of-care (POC)
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