title: Yeast Associated with the Ambrosia Beetle, Platypus koryoensis, the Pest of Oak Trees in Korea.
abstract: Oak tree death caused by symbiosis of an ambrosia beetle, Platypus koryoensis, and an ophiostomatoid filamentous fungus, Raffaelea quercus-mongolicae, has been a nationwide problem in Korea since 2004. In this study, we surveyed the yeast species associated with P. koryoensis to better understand the diversity of fungal associates of the beetle pest. In 2009, a total of 195 yeast isolates were sampled from larvae and adult beetles (female and male) of P. koryoensis in Cheonan, Goyang, and Paju; 8 species were identified by based on their morphological, biochemical and molecular analyses. Meyerozyma guilliermondii and Candida kashinagacola were found to be the two dominant species. Among the 8 species, Candida homilentoma was a newly recorded yeast species in Korea, and thus, its mycological characteristics were described. The P. koryoensis symbiont R. quercusmongolicae did not show extracelluar CM-cellulase, xylanase and avicelase activity that are responsible for degradation of wood structure; however, C. kashinagacola and M. guilliermondii did show the three extracellular enzymatic activities. Extracelluar CM-cellulase activity was also found in Ambrosiozyma sp., C. homilentoma, C. kashinagacola, and Candida sp. Extracelluar pectinase activity was detected in Ambrosiozyma sp., C. homilentoma, Candida sp., and M. guilliermondii. All the 8 yeast species displayed compatible relationships with R. quercus-mongolicae when they were co-cultivated on yeast extract-malt extract plates. Overall, our results demonstrated that P. koryoensis carries the yeast species as a symbiotic fungal associate. This is first report of yeast diversity associated with P. koryoensis.
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
authors.forename: Yeo Hong
authors.initials: YH
authors.lastname: Yun
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
authors.forename: Dong Yeon
authors.initials: DY
authors.lastname: Suh
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
authors.forename: Hun Dal
authors.initials: HD
authors.lastname: Yoo
authors.affiliation: Department of NanoBioMedical Science, Dankook University, Cheonan 31116, Korea.
authors.forename: Man Hwan
authors.initials: MH
authors.lastname: Oh
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
keywords: Oak tree death; Platypus koryoensis; Raffaelea quercus-mongolicae; Yeast
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