title: Notch signaling in the epididymal epithelium regulates sperm motility and is transferred at a distance within epididymosomes.
abstract: Spermatozoa undergo sequential maturation changes during their transit along the epididymis. These changes are modulated by the epididymal epithelium and require a finely tuned gene expression. The Notch cell signaling pathway is a major regulator of cell fate decisions in several tissues, including the testis. Here, we evaluated the transcription and expression patterns of Notch components (Notch1-3, Dll1, Dll4, and Jagged1) and effectors (Hes1-2 and Hes5) in the adult mouse epididymis, and evaluated the role of Notch signaling in the epididymis through its in vivo blockade following administration of an inhibitor (DAPT). Notch components and effectors were dynamically transcribed and expressed in the epididymis and vas deferens, each segment exhibiting a specific combination of epithelial receptor/ligand/effector expression patterns. Nuclear detection of Notch effectors indicates that Notch signaling was active. Notch components (but not effectors) were identified in the cytoplasmic droplet of spermatozoa, in a dynamic and specific pattern along the epididymis. In addition, Notch components were identified within large and small vesicles in the epididymal lumen. A purified population of these membranous vesicles from different epididymal segments was obtained, and through dot blot analysis, it was confirmed that Notch components were carried within these vesicles in a dynamic pattern along the epididymal lumen. We hypothesize that these vesicles (epididymosomes) allow Notch signaling at distance from epididymal epithelial cells to spermatozoa. DAPT-induced in vivo Notch signaling blockade, although showing a low efficiency, disrupted the expression patterns of Notch components and effectors in the epididymal epithelium and in spermatozoa, and significantly decreased sperm motility, although not affecting male fertility. These results prompt for a regulatory role of Notch signaling in epididymal epithelial function and sperm maturation.
authors.affiliation: Reproduction and Development, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.|CBIOS - Research Centre for Biosciences and Health Technologies, Faculty of Veterinary Medicine, Lusófona University of Humanities and Technologies, Lisboa, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Murta
authors.affiliation: Reproduction and Development, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Batista
authors.affiliation: Reproduction and Development, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Silva
authors.affiliation: Reproduction and Development, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.|Gulbenkian Institute of Science, Oeiras, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Trindade
authors.affiliation: Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Henrique
authors.affiliation: Reproduction and Development, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.|Gulbenkian Institute of Science, Oeiras, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Duarte
authors.affiliation: Reproduction and Development, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Lopes-da-Costa
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D004151:Dipeptides / Q000494:pharmacology; D004822:Epididymis / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D051880:Receptors, Notch / Q000378:metabolism*; D013079:Sperm Maturation / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology*; D014649:Vas Deferens / Q000378:metabolism
chemical_list: D053829:Amyloid Precursor Protein Secretases; D051792:Basic Helix-Loop-Helix Proteins; D004151:Dipeptides; D051880:Receptors, Notch; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester
keywords: DAPT; Notch; epididymis; mouse; spermatozoa