title: Electroactive polyurethane/siloxane derived from castor oil as a versatile cardiac patch, part II: HL-1 cytocompatibility and electrical characterizations.
abstract: In first part of this experiment, biocompatibility of the newly developed electroactive polyurethane/siloxane films containing aniline tetramer moieties was demonstrated with proliferation and differentiation of C2C12 myoblasts. Here we further assessed the cytocompatibility of the prepared samples with HL1-cell line, the electrophysiological properties and the patch clamp recording of the seeded cells over the selected electroactive sample. Presence of electroactive aniline tetramer in the structure of polyurethane/siloxane led to the increased expression of cardiac-specific genes of HL-1 cells involved in muscle contraction and electrical coupling. Our results showed that expression of Cx43, TrpT-2, and SERCA genes was significantly increased in conductive sample compared to tissue culture plate and the corresponding non-conductive analogous. The prepared materials were not only biocompatible in terms of cellular toxicity, but did not alter the intrinsic electrical characteristics of HL-1 cells. Embedding the electroactive moiety into the prepared films improved the properties of these polymeric cardiac construct through the enhanced transmission of electrical signals between the cells. Based on morphological observation, calcium imaging and electrophysiological recordings, we demonstrated the potential applicability of these materials for cardiac tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1398-1407, 2016.
authors.affiliation: Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Baheiraei
authors.affiliation: Department of Polyurethane, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Gharibi
authors.affiliation: Department of Polyurethane, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Yeganeh
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Milan, Italy.|CERT, Center of Excellence for Toxicological Research, Dept. of Clinical and Experimental Medicine, University of Parma, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Miragoli
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Milan, Italy.|Institute of Genetic and Biomedical Research-UOS Milan, National Research Council, Milan, Italy.
authors.forename: Nicolò
authors.initials: N
authors.lastname: Salvarani
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Milan, Italy.|Institute of Genetic and Biomedical Research-UOS Milan, National Research Council, Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Di Pasquale
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Milan, Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Condorelli
mesh_terms: D000818:Animals; D002368:Castor Oil / Q000737:chemistry*; D002460:Cell Line; D048430:Cell Shape; D004553:Electric Conductivity; D055724:Electrophysiological Phenomena; D005786:Gene Expression Regulation; D006321:Heart / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D011140:Polyurethanes / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D012833:Siloxanes / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D011140:Polyurethanes; D012833:Siloxanes; D002368:Castor Oil
keywords: cardiac patch; electroactivity; electrophysiology; myocardial infarction; polyurethane; tissue engineering