title: Gene expression and proteomic analysis of shoot apical meristem transition from dormancy to activation in Cunninghamia lanceolata (Lamb.) Hook.
abstract: In contrast to annual plants, in perennial plants, the shoot apical meristem (SAM) can undergo seasonal transitions between dormancy and activity; understanding this transition is crucial for understanding growth in perennial plants. However, little is known about the molecular mechanisms of SAM development in trees. Here, light and transmission electron microscopy revealed that evident changes in starch granules, lipid bodies, and cell walls thickness of the SAM in C. lanceolata during the transition from dormancy to activation. HPLC-ESI-MS/MS analysis showed that levels of indole-3-acetic acid (IAA) increased and levels of abscisic acid (ABA) decreased from dormant to active stage. Examination of 20 genes and 132 differentially expressed proteins revealed that the expression of genes and proteins potentially involved in cell division and expansion significantly increased in the active stage, whereas those related to the abscisic acid insensitive 3(ABI3), the cytoskeleton and energy metabolism decreased in the dormant stage. These findings provide new insights into the complex mechanism of gene and protein expression and their relation to cytological and physiological changes of SAM in this coniferous species.
authors.affiliation: Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Dechang
authors.initials: D
authors.lastname: Cao
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Science, Taizhou University, Zhejiang 318000, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wei
authors.affiliation: Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA.
authors.forename: Rebecca Ann
authors.initials: RA
authors.lastname: Stevenson
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA.
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lin
mesh_terms: D032486:Cunninghamia / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018519:Meristem / Q000378:metabolism*; D057445:Plant Dormancy / Q000502:physiology*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics
chemical_list: D010940:Plant Proteins
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