title: Determination of malachite green in aquatic products based on magnetic molecularly imprinted polymers.
abstract: Magnetic molecularly imprinted polymers (MMIPs) were synthesized through precipitation polymerization using malachite green (MG) as template, methacrylic acid as monomer, ethylene dimethacrylate as crosslinker, and Fe3O4 magnetite as magnetic component. MMIPs were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, and vibrating sample magnetometry. Under the optimum condition, the MMIPs obtained exhibited quick binding kinetics and high affinity to MG in the solution. Scatchard plot analysis revealed that the MMIPs contained only one type of binding site with dissociation constant of 24.0 μg mL(-1). The selectivity experiment confirmed that the MMIPs exhibited higher selective binding capacity for MG than its structurally related compound (e.g., crystal violet). As a sorbent for the extraction of MG in sample preparation, MMIPs together with the absorbed analytes could easily be separated from the sample matrix with an external magnet. After elution with methanol/acetic acid (9:1, v/v), MG in the eluent was determined by high-performance liquid chromatography coupled with UV detector with recoveries of 94.0-115%. Results indicated that the as-prepared MMIPs are promising materials for MG analysis in aquatic products.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China. Electronic address: linzz@jmu.edu.cn.
authors.forename: Zheng-zhong
authors.initials: ZZ
authors.lastname: Lin
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address: 1010180258@qq.com.
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China. Electronic address: ahpeng@jmu.edu.cn.
authors.forename: Ai-hong
authors.initials: AH
authors.lastname: Peng
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address: linyid@qq.com.
authors.forename: Yi-dong
authors.initials: YD
authors.lastname: Lin
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address: 776823248@qq.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361102, China. Electronic address: zhyhuang@jmu.edu.cn.
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000737:chemistry*
chemical_list: D011108:Polymers; D012394:Rosaniline Dyes; C005095:malachite green
keywords: 2,2′-Azobis(2-methylpropionitrile) (PubChem CID: 6547); Aquatic products; Ethylene dimethacrylate (PubChem CID: 7355); High-performance liquid chromatography; Magnetic molecularly imprinted polymers; Malachite green; Malachite green (PubChem CID: 11294); Methacrylic acid (PubChem CID: 4093)