title: First detection of koi herpesvirus from koi, Cyprinus carpio L. experiencing mass mortalities in Iran: clinical, histopathological and molecular study.
abstract: Koi herpesvirus (KHV) is the aetiological agent of an emerging disease (KHVD) associated with mass mortalities in koi and common carp and reported from at least 30 countries. We report the first detection of KHV from koi in Iran using clinical, histopathological and molecular studies. KHV-infected fish showed reduced swimming activity, sunken eyes and increased mucus production on skin and fins. On post-mortem examination, gill necrosis was observed in the majority of fish. Histopathologically, the gill showed diffuse necrosis of the branchial epithelial cells. Margination of chromatin was detected in gills, kidney, heart, spleen, intestine and brain. In addition, sequence analyses of the TK gene, ORF 136 and marker I and II, demonstrates that Iranian KHV isolates were identical and classified as variant A1 of TUSMT1 (J strain) and displayed the I(++) II(+) allele of this Asian genotype.
authors.affiliation: Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Rahmati-Holasoo
authors.affiliation: Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Zargar
authors.affiliation: Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Ahmadivand
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Shokrpoor
authors.affiliation: Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Ezhari
authors.affiliation: Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: H A
authors.initials: HA
authors.lastname: Ebrahimzadeh Mousavi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps; D005260:Female; D005393:Fish Diseases / Q000473:pathology* / Q000821:virology; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D007492:Iran; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Iran; TK gene; gill necrosis; koi herpesvirus