title: Review of lignocellulolytic enzyme activity analyses and scale-down to microplate-based assays.
abstract: With the increasing use of enzymes in environmental applications, there is a need for analytical methods adapted to large factorial experiments. Existing reference methods are chemical and labor intensive and unsuitable to analyze in parallel a large number of samples. Based on an extensive literature review and on experimental results, this work compares reference and microplate adapted methods to define the most adequate filter paper, carboxymethylcellulase, β-glucosidase and xylanase activity tests. In the adapted methods, the total reaction volume was reduced from 2.2-24.5 mL to 0.21-0.24 mL. Statistical analysis of the activities measured on enzyme mixtures by applying the 96-well plate reduced methods showed that they were not significantly different to the activities obtained with reference tests.
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France. Electronic address: alicia.mansour@veolia.com.
authors.forename: A A
authors.initials: AA
authors.lastname: Mansour
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France.
authors.forename: A
authors.initials: A
authors.lastname: Da Costa
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France.
authors.forename: T
authors.initials: T
authors.lastname: Arnaud
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France.
authors.forename: T A
authors.initials: TA
authors.lastname: Lu-Chau
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Fdz-Polanco
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France.
authors.forename: M T
authors.initials: MT
authors.lastname: Moreira
authors.affiliation: VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France.
authors.forename: J A
authors.initials: JA
authors.lastname: Cacho Rivero
mesh_terms: D001681:Biological Assay; D002480:Cellulase / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D008851:Micropore Filters; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase; D043364:Endo-1,4-beta Xylanases
keywords: 96-well plate; Carboxymethylcellulase; Filter paper activity; Reference methods; Xylanase; β-glucosidase