title: [Drug-resistance characteristics of CRF01_AE and CRF07_BC subtypes of HIV-1 strains in Sichuan province].
abstract: OBJECTIVE
  To investigate the drug resistance of HIV patients to the HIV-1 CRF01_AE and CRF07_BC strains in Sichuan province during 2010 to 2013.
  
  
  METHODS
  1.5 ml of plasma were collected from AIDS patients who had been receiving anti-retroviral treatment for over 6 months but still had a HIV-1 virus load of over 1 000 copies/ml from January 1, 2010 to December 31, 2013 in Sichuan province. Genetic analysis of the HIV-1 pol gene was performed using self-established method, and patients with a positive drug-resistant HIV-1 pol gene mutation were included. HIV-1 poly gene was successfully sequenced for a total of 1 213 patients. Drug resistance of different HIV-1 strains was compared with χ2 test or Fisher exact test.
  
  
  RESULTS
  558 cases (46.0%) of the 1 213 successfully sequenced patients were infected by HIV-1-strains with drug-resistant mutations, including 327 cases (58.6%) infected by CRF01_AE strain, 126 (22.6%) by CRF07_BC strain, 46 (8.2%) by CRF08_BC strain, 33 (5.9%) by B strain, 4 (0.7%) by C strain, 1 (0.2%) by CRF02_AG strain, and 21 (3.8%) by unidentified strains. Drug-resistant mutation analysis revealed that L33, F116, L74, Q151, and T69 resistance mutations occurred only in the CRF01_AE strain, while A71, K43, and Q58 resistance mutations occurred only in the CRF07_BC strain; in nuclear nucleoside reverse transcriptase inhibitors (NRTIs) and non nucleoside reverse transcriptase inhibitors (NNRTIs), CRF01_AE subtype strains showed highly resistant rate were higher than CRF07_BC, CRF08_BC and B subtype strains, with the differences were statistically significant (P<0.05).
  
  
  CONCLUSIONS
  The drug-resistant HIV-1 strains in Sichuan mainly included the CRF01_AE and CRF07_BC strains, which had different resistance mutations.
authors.affiliation: Institute of STD/AIDS Control and Prevention, Sichuan Center for Disease Control and Prevention, Chengdu 610041, China; West China School of Public Heath, Sichuan University, Chengdu 610041, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yuan
authors.forename: Ling
authors.initials: L
authors.lastname: Su
authors.forename: Honglu
authors.initials: H
authors.lastname: Liu
authors.forename: Hang
authors.initials: H
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.forename: Dongbing
authors.initials: D
authors.lastname: Wei
authors.affiliation: Email: xxpeiscu@163.com.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Pei
authors.affiliation: Email: liangshu523@163.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Liang
mesh_terms: D001483:Base Sequence; D024882:Drug Resistance, Viral; D015751:Genes, pol; D015658:HIV Infections; D015497:HIV-1; D006801:Humans; D009154:Mutation; D018894:Reverse Transcriptase Inhibitors; D019562:Viral Load
chemical_list: D018894:Reverse Transcriptase Inhibitors