title: Development of meniscus substitutes using a mixture of biocompatible polymers and extra cellular matrix components by electrospinning.
abstract: Despite the significant advances in the meniscus tissue engineering field, it is difficult to recreate the complex structure and organization of the collagenous matrix of the meniscus. In this work, we developed a meniscus prototype to be used as substitute or scaffold for the regeneration of the meniscal matrix, recreating the differential morphology of the meniscus by electrospinning. Synthetic biocompatible polymers were combined with the extracellular matrix component, collagen and used to replicate the meniscus. We studied the correlation between mechanical and structural properties of the polymer blend as a function of collagen concentration. Fibers were collected on a surface of a rapidly rotating precast mold, to accurately replicate each sectional morphology of the meniscus; different electro-tissues were produced. Detailed XRD analyses exhibited structural changes developed by electrospinning. We achieved to integrate all these electro-tissues to form a complete synthetic meniscus. Vascularization tests were performed to assess the potential use of our novel polymeric blend for promising meniscus regeneration.
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México, Campus Juriquilla, Blvd. Juriquilla 3000, C.P. 76230, Querétaro, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: López-Calzada
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México, Campus Juriquilla, Blvd. Juriquilla 3000, C.P. 76230, Querétaro, Mexico.
authors.forename: A R
authors.initials: AR
authors.lastname: Hernandez-Martínez
authors.affiliation: Universidad del Valle de México, Campus Querétaro, Blvd. Villas del Mesón 1000, C.P 76230, Querétaro, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Cruz-Soto
authors.affiliation: Ciencias de la Tierra, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hgo, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Ramírez-Cardona
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México, Campus Juriquilla, Blvd. Juriquilla 3000, C.P. 76230, Querétaro, Mexico.
authors.forename: D
authors.initials: D
authors.lastname: Rangel
authors.affiliation: Licenciatura en Tecnología, Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México, Campus Juriquilla, Blvd. Juriquilla 3000, C.P. 76230, Querétaro, Mexico.
authors.forename: G A
authors.initials: GA
authors.lastname: Molina
authors.affiliation: Centro de Investigación y de Estudios Avanzados del IPN, (CINVESTAV), Unidad Querétaro, Libramiento Norponiente #2000, Real de Juriquilla, C.P. 76230, Querétaro, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Luna-Barcenas
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México, Campus Juriquilla, Blvd. Juriquilla 3000, C.P. 76230, Querétaro, Mexico. Electronic address: miries@fata.unam.mx.
authors.forename: M
authors.initials: M
authors.lastname: Estevez
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D019245:Compressive Strength; D055119:Elastic Modulus; D005260:Female; D007668:Kidney / Q000473:pathology; D019344:Lactic Acid / Q000737:chemistry; D008592:Menisci, Tibial / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen
keywords: Biological implants; Collagen; Electrospinning; Meniscus; Tissue-based implants