title: Expression and Presence of OPG and RANKL mRNA and Protein in Human Periodontal Ligament with Orthodontic Force.
abstract: OBJECTIVE
  The objective of this study is to investigate the expression and concentration of ligand receptor activator of NFkB (RANKL) and osteoprotegerin (OPG) in human periodontal ligament (hPDL) with orthodontic forces of different magnitudes.
  
  
  METHODS
  Right premolars in 32 patients were loaded with 4oz or 7oz of orthodontic force for 7 days. Left first premolars were not loaded. After 7 days, premolars were extracted for treatment as indicated. OPG and RANKL mRNA expressions were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and ELISA was used to assess OPG and RANKL protein concentration in compression and tension sides of PDL. Data were subjected to analysis of variance and Tukey tests.
  
  
  RESULTS
  There was statistically significant difference in RANKL concentration on comparing control teeth with tension and compression sides of the experimental teeth (P < 0.0001). The expression of mRNA RANKL was increased in the tension and compression sides with 4oz (P < 0.0001). OPG did not show statistically significant association with any group. Changes in RANKL/OPG protein ratio in experimental and control groups showed statistically significant difference (P < 0.0001).
  
  
  CONCLUSIONS
  RANKL protein levels are elevated in hPDL loaded with orthodontic forces, suggesting that RANKL protein contributes to bone modeling in response to the initial placement of orthodontic force.
authors.affiliation: Director, Dental Center Research, Pontificia Universidad Javeriana, Bogotá, Colombia.; Professor, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Liliana
authors.initials: L
authors.lastname: Otero
authors.affiliation: Professor, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Dabeiba Adriana
authors.initials: DA
authors.lastname: García
authors.affiliation: Master of Science Student, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Liseth
authors.initials: L
authors.lastname: Wilches-Buitrago
keywords: OPG; RANKL; bone modeling; orthodontic forces
references.citation: J Electron Microsc (Tokyo). 2001;50(4):365-9
references.pmid: 11592682
references.citation: Angle Orthod. 2009 Nov;79(6):1108-13
references.pmid: 19852601
references.citation: Exp Mol Pathol. 2004 Jun;76(3):205-11
references.pmid: 15126102
references.citation: J Cell Biochem. 2010 May 15;110(2):304-10
references.pmid: 20225238
references.citation: Eur J Oral Sci. 2007 Oct;115(5):355-62
references.pmid: 17850423
references.citation: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2001 Dec;145(2):61-4
references.pmid: 12426773
references.citation: Angle Orthod. 2013 Jan;83(1):63-9
references.pmid: 22716279
references.citation: Trends Mol Med. 2006 Jan;12(1):17-25
references.pmid: 16356770
references.citation: Clin Oral Investig. 2016 Apr;20(3):621-9
references.pmid: 26243456
references.citation: J Orofac Orthop. 2012 Sep;73(5):397-412
references.pmid: 22955577
references.citation: Cell Metab. 2005 Dec;2(6):344-5
references.pmid: 16330319
references.citation: Am J Orthod Dentofacial Orthop. 2006 Apr;129(4):469.e1-32
references.pmid: 16627171
references.citation: Arch Oral Biol. 2012 Oct;57(10):1377-84
references.pmid: 22884709
references.citation: Orthod Craniofac Res. 2009 Aug;12(3):236-42
references.pmid: 19627526
references.citation: Angle Orthod. 2014 Jan;84(1):120-6
references.pmid: 23687969
references.citation: Prog Orthod. 2014 Dec 09;15:65
references.pmid: 25487828
references.citation: J Cell Physiol. 2008 Jan;214(1):117-25
references.pmid: 17620297
references.citation: Am J Orthod Dentofacial Orthop. 2013 Aug;144(2):203-9
references.pmid: 23910201
references.citation: Am J Orthod Dentofacial Orthop. 2013 Jan;143(1):92-100
references.pmid: 23273364
references.citation: Anat Rec. 2002 Apr 1;266(4):218-25
references.pmid: 11920384
references.citation: Am J Pathol. 2007 Feb;170(2):427-35
references.pmid: 17255310
references.citation: Gene Ther. 2006 Apr;13(8):678-85
references.pmid: 16397507
references.citation: Connect Tissue Res. 2010 Oct;51(5):359-69
references.pmid: 20497006
references.citation: Arch Oral Biol. 2007 Mar;52(3):244-50
references.pmid: 17101113
references.citation: J Cell Biochem. 2015 Dec;116(12 ):2724-9
references.pmid: 26096594
references.citation: Stomatologija. 2010;12(4):99-104
references.pmid: 21266833
references.citation: J Dent Res. 2004 Dec;83(12 ):920-5
references.pmid: 15557398
references.citation: J Periodontal Res. 2004 Feb;39(1):42-9
references.pmid: 14687227
references.citation: Oral Dis. 2002 May;8(3):147-59
references.pmid: 12108759
references.citation: Arch Biochem Biophys. 2008 May 15;473(2):139-46
references.pmid: 18395508
references.citation: Eur J Orthod. 2013 Oct;35(5):644-51
references.pmid: 22987319
references.citation: J Cell Biochem. 2011 Oct;112(10):2891-901
references.pmid: 21618593
references.citation: Bone. 2003 Jan;32(1):1-7
references.pmid: 12584029
references.citation: Orthod Craniofac Res. 2009 May;12 (2):113-9
references.pmid: 19419454
references.citation: Am J Orthod Dentofacial Orthop. 2008 May;133(5):699-707
references.pmid: 18456143
references.citation: Am J Orthod Dentofacial Orthop. 2012 Jan;141(1):30-40
references.pmid: 22196183
references.citation: Orthod Craniofac Res. 2006 Aug;9(3):137-42
references.pmid: 16918678
references.citation: Angle Orthod. 2008 Nov;78(6):988-93
references.pmid: 18947279
references.citation: Orthod Craniofac Res. 2006 May;9(2):63-70
references.pmid: 16764680
references.citation: J Bone Miner Metab. 2000;18(4):177-84
references.pmid: 10874596