title: Enhanced butanol production by increasing NADH and ATP levels in Clostridium beijerinckii NCIMB 8052 by insertional inactivation of Cbei_4110.
abstract: Clostridium beijerinckii is identified as a promising Clostridium strain for industrialization of acetone and butanol (AB) fermentation. It has been reported that high reducing power levels are associated with high butanol yield. In this study, we regulated reducing power by blocking NAD(P)H consumption in C. beijerinckii NCIMB 8052. Gene Cbei_4110, encoding NADH-quinone oxidoreductase (nuoG), is a subunit of the electron transport chain complex I. After inactivation of gene Cbei_4110, the generated mutant strain exhibited a remarkable increase in glucose utilization ratio and enhanced butanol production to 9.5 g/L in P2 medium containing 30 g/L of glucose. NAD(P)H and ATP levels were also increased by one to two times and three to five times, respectively. Furthermore, a comparative transcriptome analysis was carried out in order to determine the mechanism involved in the enhanced activity of the Cbei_4110-inactivated mutant strain. This strategy may be extended for making industrial bio-butanol more economically attractive.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China. guoting@hotmail.com.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China. guoting@hotmail.com.|Guangzhou Sugarcane Industry Research Institute, Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangdong Engineering Research & Development Center for Comprehensive Utilization of Plant Fiber, Guangzhou Key Laboratory for Comprehensive Utilization of Plant Fiber, No. 10, Shiliu gang Road, Guangzhou, 510316, China. guoting@hotmail.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China.
authors.forename: Huanqing
authors.initials: H
authors.lastname: Niu
authors.affiliation: Guangzhou Sugarcane Industry Research Institute, Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangdong Engineering Research & Development Center for Comprehensive Utilization of Plant Fiber, Guangzhou Key Laboratory for Comprehensive Utilization of Plant Fiber, No. 10, Shiliu gang Road, Guangzhou, 510316, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China. yinghanjie@njtech.edu.cn.|National Engineering Technique Research Center for Biotechnology, Nanjing, 211816, People's Republic of China. yinghanjie@njtech.edu.cn.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D042967:Electron Transport Complex I / Q000235:genetics*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007218:Industrial Microbiology; D016254:Mutagenesis, Insertional; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D059467:Transcriptome
chemical_list: D000440:Butanols; D003470:Culture Media; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D042967:Electron Transport Complex I
keywords: AB fermentation; ATP; C. beijerinckii; NAD(P)H; hydA; nuoG