title: Bt proteins Cry1Ah and Cry2Ab do not affect cotton aphid Aphis gossypii and ladybeetle Propylea japonica.
abstract: Plant varieties expressing the Bt (Bacillus thuringiensis) insecticidal proteins Cry1Ah and Cry2Ab have potential commercialization prospects in China. However, their potential effects on non-target arthropods (NTAs) remain uncharacterized. The cotton aphid Aphis gossypii is a worldwide pest that damages various important crops. The ladybeetle Propylea japonica is a common and abundant natural enemy in many cropping systems in East Asia. In the present study, the effects of Cry1Ah and Cry2Ab proteins on A. gossypii and P. japonica were assessed from three aspects. First, neither of the Cry proteins affected the growth or developmental characteristics of the two test insects. Second, the expression levels of the detoxification-related genes of the two test insects did not change significantly in either Cry protein treatment. Third, neither of the Cry proteins had a favourable effect on the expression of genes associated with the amino acid metabolism of A. gossypii and the nutrition utilization of P. japonica. In conclusion, the Cry1Ah and Cry2Ab proteins do not appear to affect the cotton aphid A. gossypii or the ladybeetle P. japonica.
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Lv
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Jin-Jie
authors.initials: JJ
authors.lastname: Cui
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Lei
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001042:Aphids / Q000187:drug effects* / Q000502:physiology*; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D001517:Coleoptera / Q000187:drug effects* / Q000502:physiology*; D004731:Endotoxins / Q000008:administration & dosage / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D017344:Genes, Insect; D003368:Gossypium / Q000235:genetics / Q000469:parasitology; D006460:Hemolysin Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D008658:Inactivation, Metabolic / Q000235:genetics; D007300:Insect Control; D030821:Plants, Genetically Modified
chemical_list: D000596:Amino Acids; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
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