title: Baicalin protects against thrombin induced cell injury in SH-SY5Y cells.
abstract: Baicalin, an extract from the dried root of Scutellaria baicalensis Georgi, was shown to be neuroprotective. However, the precise mechanisms are incompletely known. In this study, we determined the effect of baicalin on thrombin induced cell injury in SH-SY5Y cells, and explored the possible mechanisms. SH-SY5Y cells was treated with thrombin alone or pre-treated with baicalin (5, 10, 20 μM) for 2 h followed by thrombin treatment. Cells without thrombin and baicalin treatment were used as controls. Cell viability was detected by MTT assay. Cell apoptosis was analyzed by flow cytometry. Real-time PCR was performed to determine the mRNA expression of protease-activated receptor-1 (PAR-1). Western blotting was conducted to determine the protein expression of PAR-1, Caspase-3 and NF-κB. Baicalin reduced cell death following thrombin treatment in a dose-dependent manner, with concomitant inhibition of NF-κB activation and suppression of PAR-1 expression. In addition, baicalin reduced Caspase-3 expression. The above findings indicated that baicalin prevents against cell injury after thrombin stimulation possibly through inhibition of PAR-1 expression and NF-κB activation.
authors.affiliation: Department of Neurology, The Second Hospital of Shandong University Jinan 250033, China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Ju
authors.affiliation: Department of Endocrinology, Qingdao Municipal Hospital Qingdao 260071, China.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Mu
authors.affiliation: Department of Endocrinology, Qingdao Municipal Hospital Qingdao 260071, China.
authors.forename: Yuan-Tao
authors.initials: YT
authors.lastname: Liu
authors.affiliation: Department of Neurology, People's Hospital of Yishui City Yishui 276400, China.
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Wang
authors.affiliation: Central Laboratory, The Second Hospital of Shandong University Jinan 250033, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Kong
authors.affiliation: Central Laboratory, The Second Hospital of Shandong University Jinan 250033, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Neurology, The Second Hospital of Shandong University Jinan 250033, China.
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D044463:Receptor, PAR-1 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013917:Thrombin / Q000633:toxicity*; D010975:Platelet Aggregation Inhibitors
chemical_list: D005419:Flavonoids; D016328:NF-kappa B; D018696:Neuroprotective Agents; D044463:Receptor, PAR-1; C038044:baicalin; D013917:Thrombin; C505407:CASP3 protein, human; D053148:Caspase 3; D010975:Platelet Aggregation Inhibitors
keywords: Baicalin; neuroprotection; protease-activated receptor-1; thrombin
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