title: Porcine parvovirus as a contaminant in cell cultures and laboratory supplies.
abstract: Although PPV has been described as a cellular contaminant, few recent studies about the presence of this virus in cell cultures, serum, and trypsin were found in the literature. The purpose of this study was to detect the presence of porcine parvovirus (PPV) by polymerase chain reaction (PCR) in cell cultures, serum, and trypsin used in official public laboratories of educational institutes and research centers. We tested samples of cell cultures (88), batches of trypsin (10), and fetal bovine serum (13) from different manufacturers. The PCR for beta-actin and GAPDH was used to evaluate the efficiency of DNA extraction from samples. The PPV DNA was detected in 52 of 88 (59.1%) cell culture samples. One in ten batches of trypsin tested for PPV DNA was positive. In no sample of fetal bovine serum, amplification of PPV DNA was observed. Positive samples were tested and confirmed by another analyst. In addition, all positive samples were sequenced. Our results indicate that regular PCR testing for PPV in cell cultures and their supplies is important.
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Tatiana Flávia
authors.initials: TF
authors.lastname: Pinheiro de Oliveira
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Antônio Augusto
authors.initials: AA
authors.lastname: Fonseca Júnior
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Marcelo Fernandes
authors.initials: MF
authors.lastname: Camargos
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Anapolino Macedo
authors.initials: AM
authors.lastname: de Oliveira
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Nayara Fernanda
authors.initials: NF
authors.lastname: Lima
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Michele Eduardo
authors.initials: ME
authors.lastname: Freitas
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Estefânia
authors.initials: E
authors.lastname: de Oliveira Guedes
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Isabela Ciarlini
authors.initials: IC
authors.lastname: de Azevedo
authors.affiliation: Laboratório Nacional Agropecuário de Minas Gerais (LANAGRO-MG), Pedro Leopoldo, MG, Brazil.
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Pinto Cottorello
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brazil. Electronic address: marcosbryan@usp.br.
authors.forename: Marcos Bryan
authors.initials: MB
authors.lastname: Heinemann
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D004279:DNA, Viral / Q000235:genetics*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000235:genetics*; D028323:Parvovirus, Porcine / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral
keywords: Cell culture; PCR; Phylogenetic analysis; Porcine parvovirus; Trypsin