title: Lycopene Prevents Amyloid [Beta]-Induced Mitochondrial Oxidative Stress and Dysfunctions in Cultured Rat Cortical Neurons.
abstract: Brains affected by Alzheimer's disease (AD) show a large spectrum of mitochondrial alterations at both morphological and genetic level. The causal link between β-amyloid (Aβ) and mitochondrial dysfunction has been established in cellular models of AD. We observed previously that lycopene, a member of the carotenoid family of phytochemicals, could counteract neuronal apoptosis and cell damage induced by Aβ and other neurotoxic substances, and that this neuroprotective action somehow involved the mitochondria. The present study aims to investigate the effects of lycopene on mitochondria in cultured rat cortical neurons exposed to Aβ. It was found that lycopene attenuated Aβ-induced oxidative stress, as evidenced by the decreased intracellular reactive oxygen species generation and mitochondria-derived superoxide production. Additionally, lycopene ameliorated Aβ-induced mitochondrial morphological alteration, opening of the mitochondrial permeability transition pores and the consequent cytochrome c release. Lycopene also improved mitochondrial complex activities and restored ATP levels in Aβ-treated neuron. Furthermore, lycopene prevented mitochondrial DNA damages and improved the protein level of mitochondrial transcription factor A in mitochondria. Those results indicate that lycopene protects mitochondria against Aβ-induced damages, at least in part by inhibiting mitochondrial oxidative stress and improving mitochondrial function. These beneficial effects of lycopene may account for its protection against Aβ-induced neurotoxicity.
authors.affiliation: Center for Diseases Prevention and Control of the Rocket Force of PLA, Beijing, 100094, People's Republic of China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Qu
authors.affiliation: Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Center for Diseases Prevention and Control of the Rocket Force of PLA, Beijing, 100094, People's Republic of China. liaoyuanxiangsci@163.com.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Center for Diseases Prevention and Control of the Air Force of PLA, Beijing, 100076, People's Republic of China.
authors.forename: Zhenyao
authors.initials: Z
authors.lastname: Song
authors.affiliation: Center for Diseases Prevention and Control of the Rocket Force of PLA, Beijing, 100094, People's Republic of China.
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Nan
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D002338:Carotenoids / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D000077276:Lycopene; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D002338:Carotenoids; D000077276:Lycopene
keywords: Alzheimer’s disease; Lycopene; Mitochondria; Neurons; β-Amyloid
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