title: The effect of ozonated sterile saline irrigation on the endometrium - A preliminary study.
abstract: The objective of this study was to evaluate the effect of sterile ozonated saline endometrial irrigation on sonographic and histological endometrial parameters. This prospective investigation was performed in 12 healthy, ovulating women over three consecutive menstrual cycles: control cycle (endometrial irrigation with 10 cc of normal saline at day 10), no intervention cycle and study cycle (irrigation with 10 cc of sterile ozonated saline at day 10). Endometrial thickness was measured by transvaginal ultrasound at days 10 and 12 of the control and study cycles, and endometrial samplings were obtained from the participants two days after the irrigations (i.e. on day 12) for histological evaluation. Ozonated saline irrigation, compared to normal saline irrigation, resulted in a statistically significant elevation of the columnar epithelial height (30.30 ± 3.04 vs. 25.82 ± 3.28 μm, p < 0.003), increased number of endometrial blood vessels (30.48 ± 11.38 vs. 19.12 ± 8.74, p < 0.005) and increased number of stromal cells (191.30 ± 34.40 vs. 151.29 ± 29.98, p < 0.01). In conclusion, sterile ozonated saline irrigation of the endometrium has a significant favourable effect on various histological endometrial parameters. Further studies are needed to evaluate the effect of these changes on endometrial receptivity and pregnancy rates.
authors.affiliation: a IVF Unit, Department of Obstetrics and Gynecology, Bnai-Zion Medical Center and the B. Rappaport Faculty of Medicine , The Technion , Haifa , Israel .
authors.forename: Ilan
authors.initials: I
authors.lastname: Calderon
authors.affiliation: a IVF Unit, Department of Obstetrics and Gynecology, Bnai-Zion Medical Center and the B. Rappaport Faculty of Medicine , The Technion , Haifa , Israel .
authors.forename: Max
authors.initials: M
authors.lastname: Cohen
authors.affiliation: b Department of Obstetrics and Gynecology , Carmel Medical Center , Haifa , Israel .
authors.forename: Lena
authors.initials: L
authors.lastname: Sagi-Dain
authors.affiliation: c Department of Dermatology , Sheba Medical Center , Tel Hashomer, Haifa , Israel , and.
authors.forename: Ofir
authors.initials: O
authors.lastname: Artzi
authors.affiliation: d Department of Pathology , Bnai-Zion Medical Center , Haifa , Israel.
authors.forename: Jacob
authors.initials: J
authors.lastname: Bejar
authors.affiliation: a IVF Unit, Department of Obstetrics and Gynecology, Bnai-Zion Medical Center and the B. Rappaport Faculty of Medicine , The Technion , Haifa , Israel .
authors.forename: Shlomi
authors.initials: S
authors.lastname: Sagi
mesh_terms: D000293:Adolescent; D000328:Adult; D004717:Endometrium / Q000187:drug effects* / Q000502:physiology; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008597:Menstrual Cycle; D010060:Ovulation; D010126:Ozone / Q000008:administration & dosage*; D011446:Prospective Studies; D012965:Sodium Chloride / Q000008:administration & dosage*; D007507:Therapeutic Irrigation / Q000379:methods*; D055815:Young Adult
chemical_list: D012965:Sodium Chloride; D010126:Ozone
keywords: Ozone irrigation; endometrial histology; endometrial receptivity; endometrial thickness