title: Synthesis and osteo-compatibility of novel reduced graphene oxide-aminosilica hybrid nanosheets.
abstract: Combination of silica component with other materials is one of the current strategies to design bone regenerative materials. In this study, novel reduced graphene oxide (RGO)-aminosilica hybrid nanosheets with enhanced osteo-compatibility were synthesized from a mixture of 3-aminopropyltriethoxysilane (APTES), graphene oxides (GO) and water. The presence of APTES in the mixture not only caused the conversion of GO to RGO, but also led to the hydrolysis and condensation of itself. It was for the first time reported the reducing role of APTES in the conversion of GO to RGO. It was found that the silicon (IV) ions were released from the hybrid nanosheets in a sustained way. The in vitro osteo-compatibility was evaluated by incubating the hybrid nanosheets with osteoblast MC3T3-E1 cells. A water soluble tetrazolium salt assay quantitatively indicated that the hybrid nanosheets had no significant toxicity and exhibited good biocompatibility. An alkaline phosphatase assay quantitatively indicated that the hybrid nanosheets enhanced the osteoblast differentiation compared to the GO nanosheets. An immunochemical assay further qualitatively indicated that the hybrid nanosheets stimulated the production of osteopontin as typical marker for osteoblast differentiation. Thus, the resultant hybrids nanosheets had a potential application in the bone regeneration.
authors.affiliation: College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Biomaterials Unit, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 3050047, Japan. Electronic address: chensong2009@126.com.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Du
authors.affiliation: College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Jia
authors.affiliation: Center for Joining and Electronic Packaging, State Key Laboratory of Material Processing and Die & Mold Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology (HUST), Wuhan 430074, China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Chang
authors.affiliation: Graduate School of Science & Engineering, Tokyo Institute of Technology, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ikoma
authors.affiliation: Biomaterials Unit, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 3050047, Japan. Electronic address: HANAGATA.Nobutaka@nims.go.jp.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hanagata
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011437:Propylamines / Q000737:chemistry / Q000494:pharmacology; D012821:Silanes / Q000737:chemistry / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D011437:Propylamines; D012821:Silanes; D012822:Silicon Dioxide; D006108:Graphite; C477625:amino-propyl-triethoxysilane
keywords: Graphene oxides; Hybrids; Osteo-compatibility; Silica