title: Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells.
abstract: BACKGROUND
  The intestinal cells of Caenorhabditis elegans are filled with heterogeneous granular organelles that are associated with specific organ functions. The best studied of these organelles are lipid droplets and acidified gut granules associated with GLO-1, a homolog of the small GTPase Rab38. In this study, we characterized a subset of the intestinal granules in which HAF-4 and HAF-9 localize on the membrane. HAF-4 and HAF-9 are ATP-binding cassette (ABC) transporter proteins that are homologous to the mammalian lysosomal peptide transporter TAPL (transporter associated with antigen processing-like, ABCB9).
  
  
  RESULTS
  Using transgenic worms expressing fluorescent protein-tagged marker proteins, we demonstrated that the HAF-4- and HAF-9-localizing organelles are not lipid droplets and do not participate in yolk protein transport. They were also ruled out as GLO-1-positive acidified gut granules. Furthermore, we clarified that the late endosomal protein RAB-7 localizes to the HAF-4- and HAF-9-localizing organelles and is required for their biogenesis.
  
  
  CONCLUSIONS
  Our results indicate that the HAF-4- and HAF-9-localizing organelles are distinct intestinal organelles associated with the endocytic pathway.
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. ttanji@iwate-med.ac.jp.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tanji
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. knishiko@iwate-med.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Nishikori
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. s.haga0911@gmail.com.
authors.forename: Syoko
authors.initials: S
authors.lastname: Haga
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. yukikanno.p@gmail.com.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kanno
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. kobayasi504@yahoo.co.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. mai_takaya_imu@yahoo.co.jp.
authors.forename: Mai
authors.initials: M
authors.lastname: Takaya
authors.affiliation: Department of Physiology, School of Medicine, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan. andok@mail.saitama-u.ac.jp.|Present address: Saitama University Brain Science Institute, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan. andok@mail.saitama-u.ac.jp.
authors.forename: Keiko
authors.initials: K
authors.lastname: Gengyo-Ando
authors.affiliation: Department of Physiology, School of Medicine, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan. mitani.shohei@twmu.ac.jp.
authors.forename: Shohei
authors.initials: S
authors.lastname: Mitani
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. hshirais@iwate-med.ac.jp.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Shiraishi
authors.affiliation: Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, Shiwa-gun, Iwate, 028-3694, Japan. aohashi@iwate-med.ac.jp.
authors.forename: Ayako
authors.initials: A
authors.lastname: Ohashi-Kobayashi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D015388:Organelles / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D018528:ATP-Binding Cassette Transporters; D029742:Caenorhabditis elegans Proteins; C543630:HAF-4 protein, C elegans; C543631:HAF-9 protein, C elegans
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