title: Effect of GO-Fe3O4 and rotating magnetic field on cellular metabolic activity of mammalian cells.
abstract: The effect of hybrid material-graphene flakes with Fe3O4 nanospheres (GO-Fe3O4), graphene oxide (GO) and magnetite nanospheres (Fe3O4) in rotating magnetic field on mammalian cells metabolism has been studied. Several reports shown that exposure to magnetic field may have influence on cellular membrane permeability. Thus, the aim of presented study was to determine the cellular response of L929 fibroblast cells to nanomaterials and rotating magnetic field for 8-h exposure experiment. The GO had tendency to adsorb proteins, thus cell metabolism was decreased and the effect of that mechanism was enhanced by impact of nanospheres and rotating magnetic field. The highest reduction of cellular metabolism was recorded for WST-1 and NR assays at concentration 100 µg/mL of all tested nanomaterials and magnetic induction value 10.06 mT. The lactate dehydrogenase leakage assay has shown significant changes in membrane permeability. Further studies need to be carried out to precisely determine the mechanism of that process.
authors.affiliation: Department of Environmental and Chemical Engineering, West Pomeranian University of Technology, Szczecin, Poland kurbas@zut.edu.pl.
authors.forename: Karolina
authors.initials: K
authors.lastname: Urbas
authors.affiliation: Laboratory of Cytogenetics, West Pomeranian University of Technology, Szczecin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jedrzejczak-Silicka
authors.affiliation: Institute of Chemical Engineering and Environmental Protection Process, West Pomeranian University of Technology, Szczecin, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Rakoczy
authors.affiliation: Laboratory of Biostatistics, West Pomeranian University of Technology, Szczecin, Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Zaborski
authors.affiliation: Department of Environmental and Chemical Engineering, West Pomeranian University of Technology, Szczecin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Mijowska
mesh_terms: D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010087:Oxides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D058185:Magnetite Nanoparticles; D010087:Oxides; D006108:Graphite; D007770:L-Lactate Dehydrogenase
keywords: Magnetite nanospheres; graphene oxide; hyperthermia; magnetoporation; rotating magnetic field