title: Climbing Fiber Regulation of Spontaneous Purkinje Cell Activity and Cerebellum-Dependent Blink Responses(1,2,3).
abstract: It has been known for a long time that GABAergic Purkinje cells in the cerebellar cortex, as well as their target neurons in the cerebellar nuclei, are spontaneously active. The cerebellar output will, therefore, depend on how input is integrated into this spontaneous activity. It has been shown that input from climbing fibers originating in the inferior olive controls the spontaneous activity in Purkinje cells. While blocking climbing fiber input to the Purkinje cells causes a dramatic increase in the firing rate, increased climbing fiber activity results in reduced Purkinje cell activity. However, the exact calibration of this regulation has not been examined systematically. Here we examine the relation between climbing fiber stimulation frequency and Purkinje cell activity in unanesthetized decerebrated ferrets. The results revealed a gradual suppression of Purkinje cell activity, starting at climbing fiber stimulation frequencies as low as 0.5 Hz. At 4 Hz, Purkinje cells were completely silenced. This effect lasted an average of 2 min after the stimulation rate was reduced to a lower level. We also examined the effect of sustained climbing fiber stimulation on overt behavior. Specifically, we analyzed conditioned blink responses, which are known to be dependent on the cerebellum, while stimulating the climbing fibers at different frequencies. In accordance with the neurophysiological data, the conditioned blink responses were suppressed at stimulation frequencies of ≥4 Hz.
authors.affiliation: Synthetic Perceptive Emotive and Cognitive Systems Laboratory, Center for Autonomous Systems and Neuro-robotics, Information and Communication Technologies Department, Universitat Pompeu Fabra , 08002 Barcelona, Spain.
authors.forename: Riccardo
authors.identifier: 0000-0003-4808-6010
authors.initials: R
authors.lastname: Zucca
authors.affiliation: Department of Neuroscience, Erasmus Medical Center , 3000 DR, Rotterdam, The Netherlands.
authors.forename: Anders
authors.identifier: 0000-0003-4477-369X
authors.initials: A
authors.lastname: Rasmussen
authors.affiliation: Department of Experimental Medical Science, Section for Neurophysiology, Lund University , SE-221 84 Lund, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Bengtsson
mesh_terms: D000200:Action Potentials; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D003215:Conditioning, Eyelid / Q000502:physiology*; D005289:Ferrets; D008297:Male; D011689:Purkinje Cells / Q000502:physiology*
keywords: Purkinje cell; climbing fibers; eyeblink conditioning; simple spikes; spontaneous background firing
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