title: Resveratrol and its methoxy-derivatives as modulators of DNA damage induced by ionising radiation.
abstract: Various naturally occurring stilbene-like compounds that are related to resveratrol (RSV) possess some of the beneficial effects of the parent molecule and provide even further benefits. Therefore, a series of methoxylated analogues of RSV were prepared with the aim of increasing antitumour and proapoptotic activity. In a previous article, we studied two methoxy-derivatives, pterostilbene (PTERO) and trimethoxystilbene (TRIMETHOXY), in which the first was formed by the substitution of two hydroxyl groups with two methoxy groups (trans-3,5-dimethoxy-4'-hydroxystilbene) and the second was formed by the replacement of all three OH groups with methoxy groups (trans-3,5,4'-trimethoxystilbene). Both methoxy-derivatives showed stronger antioxidant activity when compared with RSV. In the present article, we focused on the analysis of the ability of RSV and its two methoxylated derivatives to protect proliferating non-tumoural cells from the damage induced by ionising radiation (IR). First we showed that the methoxy derivatives, contrary to their parental compound, are unable to affect topoisomerase enzyme and consequently are not clastogenic per se Second we showed that both PTERO and TRIMETHOXY more efficiently reduce the chromosome damage induced by IR. Furthermore, TRIMETHOXY, but not PTERO, causes a delay in cell proliferation, particularly in mitosis progression increasing the number of cells in metaphase at the expense of prophases and ana/telophases.
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G.Marconi, 446, 00146 Roma, Italy.
authors.forename: Gianandrea
authors.initials: G
authors.lastname: Traversi
authors.affiliation: Istituto di Biologia Molecolare e Patologia CNR, Via degli Apuli, 4, 00185 Roma, Italy and.
authors.forename: Mario
authors.initials: M
authors.lastname: Fiore
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G.Marconi, 446, 00146 Roma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Leone
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G.Marconi, 446, 00146 Roma, Italy.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Basso
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G.Marconi, 446, 00146 Roma, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Di Muzio
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G.Marconi, 446, 00146 Roma, Italy, Istituto Nazionale di Fisica Nucleare, Sezione di Roma Tre, Via della Vasca Navale, 84, 00146 Roma, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Polticelli
authors.affiliation: Istituto di Biologia Molecolare e Patologia CNR, Via degli Apuli, 4, 00185 Roma, Italy and.
authors.forename: Francesca
authors.initials: F
authors.lastname: Degrassi
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G.Marconi, 446, 00146 Roma, Italy, renata.cozzi@uniroma3.it.
authors.forename: Renata
authors.initials: R
authors.lastname: Cozzi
mesh_terms: D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D003412:Cricetulus / Q000235:genetics / Q000502:physiology; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D008938:Mitosis / Q000187:drug effects; D011839:Radiation, Ionizing; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000633:toxicity; D059003:Topoisomerase Inhibitors / Q000494:pharmacology
chemical_list: C497286:3,4',5-trimethoxystilbene; D013267:Stilbenes; D059003:Topoisomerase Inhibitors; C107773:pterostilbene; D004247:DNA; D000077185:Resveratrol