title: Pseudomonas populisp. nov., an endophytic bacterium isolated fromPopulus euphratica.
abstract: A Gram-stain negative, aerobic, non-motile, short-rod-shaped bacterium, designated KBL-4-9T, was isolated from the storage liquid in the stems ofPopulus euphraticatrees in Xinjiang, PR China. Strain KBL-4-9Tgrew at 4-45 °C (optimum 37 °C), 1-3 % (w/v) NaCl (optimum 1 %, w/v) and pH 5.5-9.5 (optimum pH 7.5). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9Tbelonged to the genusPseudomonasand showed the highest 16S rRNA gene sequence similarity of 97.36 % toPseudomonas pelagiaCL-AP6T, followed byPseudomonas xinjiangensisS3-3T(97.16 %),Pseudomonas sabulinigriJ64T(97.15 %) andPseudomonas xiamenensisC10-2T(96.47 %). Analysis of strain KBL-4-9Tbased on the three housekeeping genes,rpoB,rpoDandgyrB, further confirmed the phylogenetic assignment of the isolates. The DNA G+C content was 61.6 mol% (sd = 2.19). DNA-DNA hybridization withP. pelagiaCL-AP6T,P. xinjiangensisS3-3TandP. sabulinigriJ64Trevealed 49.3 % (sd = 3.04), 41.2 % and 52.5 % (sd = 4.45) relatedness, respectively. The major cellular fatty acids of strain KBL-4-9Twere C16 : 0, C12 : 0, summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c) and C17 : 0cyclo. The major isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain KBL-4-9Tis considered to represents a novel species of the genusPseudomonas, for which the namePseudomonas populisp. nov. is proposed. The type strain is KBL-4-9T( = JCM 19138T= CCTCC AB 2013069T= NRRL B-59988T).
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Nusratgul
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authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
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authors.affiliation: Department of Microbiology, Al-Farabi Kazakh National University, Almaty 480078, Kazakhstan.
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authors.lastname: Zayadan
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
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authors.lastname: Abdurahman
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Buayshem
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authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Raziya
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authors.lastname: Erkin
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
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authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Manziram
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authors.lastname: Rozahon
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Hormathan
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authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Erkin
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