title: Anti-laminin-1 antibodies in serum and follicular fluid of women with Hashimoto's thyroiditis undergoing in vitro fertilization.
abstract: The aim of this study is to evaluate the presence of anti-laminin-1 antibodies (aLN-1) in sera and follicular fluid (FF) of infertile women affected by Hashimoto's thyroiditis (HT) undergoing in vitro fertilization (IVF) and its impact on oocyte maturation and IVF outcome. aLN-1 were measured by a home-made enzyme linked immunosorbent assay (ELISA) in: (1) sera and FF from 44 infertile women affected by HT (HTIW) with tubal factor or male factor as primary cause of infertility; (2) in sera and FF from 28 infertile women without HT, with tubal factor or male factor as cause of infertility (infertile controls-ICTR); and (3) in sera from 50 fertile women (FW). aLN-1 serum levels were significantly higher in HTIW when compared with both fertile women and ICTR (P <0.001and P <0.01, respectively). Assuming as cutoff the 99th percentile of values obtained in sera of FW, 43.2% of HTIW and 3.6% of ICTR were aLN-1 positive (P = 0.0001). Also aLN-1 detected in FF from HTIW were significantly higher in comparison with those found in FF of ICTR (P = 0.006). In HTIW, metaphase II oocyte count showed inverse correlation with both serum and FF aLN-1 levels (r = 0.34, P = 0.02 and r = 0.33, P = 0.03, respectively). Implantation and pregnancy rates were significantly lower in HTIW (7.9% and 9.1%, respectively) when compared with ICTR (23% and 31.1%, respectively) (P = 0.015 and P = 0.03, respectively). Our results demonstrated for the first time the presence of aLN-1 in a relevant percentage of HTIW and suggest that these auto-antibodies may impair IVF outcome.
authors.affiliation: Department of Biomedical Sciences and Human Oncology, Section of Immunology, University of Bari "Aldo Moro", University Hospital, Italy domenico.caccavo@uniba.it.
authors.forename: Domenico
authors.initials: D
authors.lastname: Caccavo
authors.affiliation: Department of Biomedical Sciences and Human Oncology, Section of Immunology, University of Bari "Aldo Moro", University Hospital, Italy.
authors.forename: Nelly M
authors.initials: NM
authors.lastname: Pellegrino
authors.affiliation: Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Nardelli
authors.affiliation: Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Vergine
authors.affiliation: Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Leone
authors.affiliation: Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Marolla
authors.affiliation: Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy.
authors.forename: Margherita P
authors.initials: MP
authors.lastname: Vacca
authors.affiliation: Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Depalo
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D050031:Hashimoto Disease / Q000097:blood* / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000378:metabolism; D007797:Laminin / Q000037:antagonists & inhibitors*; D008297:Male; D009865:Oocytes / Q000378:metabolism; D011247:Pregnancy; D018873:Pregnancy Rate; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D007797:Laminin; C121346:laminin 1
keywords: Hashimoto’s thyroiditis; anti-laminin-1 Ab; in vitro fertilization (IVF) outcome
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