title: Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model.
abstract: Models of tumor angiogenesis have played a critical role in understanding the mechanisms involved in the recruitment of vasculature to the tumor mass, and have also provided a platform for testing antiangiogenic potential of new therapeutics that combat the development of malignant growth. In this regard, the chorioallantoic membrane (CAM) of the developing chick embryo has proven to be an elegant model for investigation of angiogenic processes. Here, we describe methods for effectively utilizing the preestablished vascular network of the chick CAM to investigate and quantify tumor-associated angiogenesis in a breast tumor model.
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME, 04074, USA.|Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, 04469, USA.
authors.forename: Jacquelyn J
authors.initials: JJ
authors.lastname: Ames
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME, 04074, USA.
authors.forename: Terry
authors.initials: T
authors.lastname: Henderson
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME, 04074, USA.
authors.forename: Lucy
authors.initials: L
authors.lastname: Liaw
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME, 04074, USA. brookp1@mmc.org.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Brooks
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D009389:Neovascularization, Pathologic; D055114:X-Ray Microtomography
keywords: 4T1 tumor cells; Angiogenesis; Breast tumors; Chick chorioallantoic membrane (CAM); Immunofluorescence (IF); Microcomputed tomography (microCT); Microfil®; Tumor angiogenesis; von Willebrand factor (vWF)