title: Ordered self-assembly of the collagenous domain of adiponectin with noncovalent interactions via glycosylated lysine residues.
abstract: Adiponectin, an anti-atherogenic and insulin-sensitizing adipokine, forms multiple isoforms including a trimer, a hexamer and heavier oligomers (mainly octadecamer) that determine their biological activities. We designed 89-residue peptides containing modifications found in the collagenous domain of native adiponectin. Circular dichroism and analytical ultracentrifugation measurements showed that the peptide bearing glucosyl-galactosyl-hydroxylysine residues forms a stable collagen-like triple helical structure and spontaneously assembled into an octadecamer. An assembly model mediated by noncovalent interactions via glycosylated lysine residues for the octadecamer was constructed. Our findings clarified an essential role of glycosyl modifications to coordinate the ordered self-assembly of adiponectin.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Takuwa
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Maruno
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kawahara
authors.affiliation: Peptide Institute Inc., SAITO Res Ctr, Ibaraki, Osaka, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: Peptide Institute Inc., SAITO Res Ctr, Ibaraki, Osaka, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nishiuchi
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: Tadayasu
authors.initials: T
authors.lastname: Ohkubo
mesh_terms: D052242:Adiponectin / Q000737:chemistry*; D019540:Area Under Curve; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry*; D006031:Glycosylation; D008239:Lysine / Q000737:chemistry*
chemical_list: D052242:Adiponectin; D003094:Collagen; D008239:Lysine
keywords: adiponectin; collagen; glycosylation; octadecamer; self-assembly