title: A Simple Method for Labeling Human Embryonic Stem Cells Destined to Lose Undifferentiated Potency.
abstract: Mitochondrial oxidative phosphorylation is a major source of cellular ATP. Its usage as an energy source varies, not only according to the extracellular environment, but also during development and differentiation, as indicated by the reported changes in the flux ratio of glycolysis to oxidative phosphorylation during embryonic stem (ES) cell differentiation. The fluorescent probe JC-1 allows visualization of changes in the mitochondrial membrane potential produced by oxidative phosphorylation. Strong JC-1 signals were localized in the differentiated cells located at the edge of H9 ES colonies that expressed vimentin, an early differentiation maker. The JC-1 signals were further intensified when individual adjacent colonies were in contact with each other. Time-lapse analyses revealed that JC-1-labeled H9 cells under an overconfluent condition were highly differentiated after subculture, suggesting that monitoring oxidative phosphorylation in live cells might facilitate the prediction of induced pluripotent stem cells, as well as ES cells, that are destined to lose their undifferentiated potency.
authors.affiliation: Laboratory of Cell Signaling and Metabolic Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Kumagai
authors.affiliation: Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Suga
authors.affiliation: Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Yanagihara
authors.affiliation: Laboratory of Cell Signaling and Metabolic Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Itoh
authors.affiliation: Laboratory of Cell Signaling and Metabolic Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan takemori@nibiohn.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takemori
authors.affiliation: Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
authors.forename: Miho K
authors.initials: MK
authors.lastname: Furue
mesh_terms: D000255:Adenosine Triphosphate; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D058948:Cell Tracking / Q000379:methods; D004734:Energy Metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D005456:Fluorescent Dyes; D014746:Vimentin; D000255:Adenosine Triphosphate
keywords: Embryonic stem cells; JC-1-labeled cells; Oxidative phosphorylation; Undifferentiated potency
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