title: Yamadazyma kitorensis f.a., sp. nov. and Zygoascus biomembranicola f.a., sp. nov., novel yeasts from the stone chamber interior of the Kitora tumulus, and five novel combinations in Yamadazyma and Zygoascus for species of Candida.
abstract: Analysis of D1/D2 large-subunit (LSU) rRNA gene sequences predicted that 17 yeast isolates, mainly from viscous gels (biofilms) taken from the stone chamber interior of the Kitora tumulus in Nara, Japan, were placed in the Yamadazyma and Zygoascus clades. Polyphasic characterization, including morphological, physiological and chemotaxonomic characteristics, multigene sequence divergence and DNA-DNA hybridization, strongly suggested the assignment of one novel species to each of the clades; these are Yamadazyma kitorensis f.a., sp. nov., with the type strain JCM 31005T (ex-type CBS 14158T=isolate K8617-6-8T), and Zygoascus biomembranicola f.a., sp. nov., with the type strain JCM 31007T (ex-type CBS 14157T=isolate K61208-2-11T). Furthermore, the transfer of five known species of the genus Candida as novel combinations to the genera Yamadazyma and Zygoascus is proposed; these are Yamadazyma olivae f.a., comb. nov. (type strain CBS 11171T=ATCC MYA-4568T), Yamadazyma tumulicola f.a., comb. nov. (type strain JCM 15403T=ex-type CBS 10917T=isolate T6517-9-5T), Yamadazyma takamatsuzukensis f.a., comb. nov. (type strain JCM 15410T=CBS 10916T = isolate T4922-1-1T), Zygoascus polysorbophila f.a., comb. nov. (type strain NRRL Y-27161T=CBS 7317T) and Zygoascus bituminiphila f.a., comb. nov. (type strain CBS 8813T=MUCL 41424T).
authors.affiliation: TechnoSuruga Laboratory Co., Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka, 424-0065, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nagatsuka
authors.affiliation: TechnoSuruga Laboratory Co., Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka, 424-0065, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Ninomiya
authors.affiliation: TechnoSuruga Laboratory Co., Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka, 424-0065, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kiyuna
authors.affiliation: Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo, 13-43 Ueno Park, Taito-ku, Tokyo 110-8713, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Kigawa
authors.affiliation: Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo, 13-43 Ueno Park, Taito-ku, Tokyo 110-8713, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Sano
authors.affiliation: TechnoSuruga Laboratory Co., Ltd, Chiba Branch Office, 3-1532-13 Hasama-cho, Funabashi-shi, Chiba 274-0822, Japan.
authors.forename: Junta
authors.initials: J
authors.lastname: Sugiyama
mesh_terms: D001482:Base Composition; D002175:Candida / Q000145:classification; D004271:DNA, Fungal / Q000235:genetics; D007564:Japan; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009693:Nucleic Acid Hybridization; D010151:Paintings; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D012335:RNA, Ribosomal