title: Curcumin inhibits the proliferation and invasion of human osteosarcoma cell line MG-63 by regulating miR-138.
abstract: OBJECTIVE
  In this study, we screened the different human osteosarcoma cell line MG-63 miRNAs after the treatment of curcumin and explored the effects of curcumin on MG-63 cells and its mechanism.
  
  
  METHODS
  Affemitrix miRNA chip was used to detect the changes of miRNA expression profile in MG-63 cells before and after curcumin treatment, and screen different expression of miRNAs. The target gene of miRNA was analyzed by bioinformatics. The expression levels of miRNA-138 target genes Smad4, NFκB p65 and cyclin D3 were detected. MTT and Transwell Cell invasion assays were used to observe the effects of curcumin on MG-63 cells.
  
  
  RESULTS
  Curcumin could significantly inhibit the proliferation of MG-63 cells and the expression levels of miRNA-138 target genes Smad4, NFκB p65 and cyclin D3 in MG-63 cells (P<0.05); overexpression of hsa-miR-138 down-regulated the expression levels of Smad4, NFκB p65 and cyclin D3 compared with the treatment of curcumin, while inhibition of hsa-miR-138 up-regulated the expression levels of Smad4, NFκB p65 and cyclin D3.
  
  
  CONCLUSIONS
  Curcumin could increase the expression of hsa-miR-138, hsa-miR-138 inhibited cell proliferation and invasive ability by inhibition of its target genes.
authors.affiliation: Department of Orthopedic and Traumatic Surgery, General Hospital of Jinan Military CommandJinan, Shandong, China; Department of Hand Surgery, 401 Hospital of PLAQingdao, Shandong, China.
authors.forename: Dazhi
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Hand Surgery, 401 Hospital of PLA Qingdao, Shandong, China.
authors.forename: Fengmei
authors.initials: F
authors.lastname: An
authors.affiliation: Department of Hand Surgery, 401 Hospital of PLA Qingdao, Shandong, China.
authors.forename: Xu
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Orthopedic and Traumatic Surgery, General Hospital of Jinan Military Command Jinan, Shandong, China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Cao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D003474:Curcumin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D012516:Osteosarcoma / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; C546110:MIRN138 microRNA, human; D035683:MicroRNAs; D003474:Curcumin
keywords: Curcumin; MG-63 cells; invasion; miR-138; miRNAs; proliferation
references.citation: Front Cell Infect Microbiol. 2014;4:165
references.pmid: 25426450
references.citation: Exp Ther Med. 2015 Jun;9(6):2374-2378
references.pmid: 26136990
references.citation: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references.pmid: 15761078
references.citation: Methods Mol Biol. 2016;1375:1-10
references.pmid: 25971910
references.citation: Cancer Genet. 2011 Mar;204(3):138-46
references.pmid: 21504713
references.citation: J Transl Med. 2012;10:228
references.pmid: 23164426
references.citation: Mol Med Rep. 2015 Sep;12(3):4554-9
references.pmid: 26081423
references.citation: J Biol Chem. 2015 Apr 17;290(16):10518-34
references.pmid: 25716318
references.citation: Biosci Rep. 2011 Jun;31(3):185-97
references.pmid: 20815812
references.citation: Anticancer Res. 2015 Jun;35(6):3235-43
references.pmid: 26026083
references.citation: Nat Methods. 2006 Nov;3(11):881-6
references.pmid: 17060911
references.citation: Tumour Biol. 2015 Jan;36(1):111-9
references.pmid: 25464861
references.citation: Biochem Biophys Res Commun. 2010 Sep 17;400(2):236-40
references.pmid: 20727858
references.citation: Int J Mol Sci. 2014;15(11):20134-57
references.pmid: 25380521
references.citation: Cell Tissue Bank. 2016 Mar;17(1):91-104
references.pmid: 26084504
references.citation: Anticancer Res. 2015 Jul;35(7):3867-73
references.pmid: 26124332
references.citation: Dev Biol. 2015 Apr 1;400(1):132-8
references.pmid: 25641697
references.citation: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4
references.pmid: 16381832
references.citation: Pharm Biol. 2016 Aug;54(8):1303-11
references.pmid: 26134921