title: Characterization of human adipose tissue-derived stem cells with enhanced angiogenic and adipogenic properties.
abstract: Autologous fat grafting is a popular method for soft tissue reconstructions but graft survival remains highly unpredictable. Supplementation of the graft with the stromal vascular fraction (SVF) or cultured adipose tissue-derived stem cells (ASCs) can enhance graft viability. In this study we have examined the phenotypic properties of a selected population of cells isolated from ASCs, with a view to determining their suitability for transplantation into grafts. ASCs were isolated from the SVF of human abdominal fat (n = 8 female patients) and CD146+cells were selected using immunomagnetic beads. The angiogenic and adipogenic properties of the positively selected cells were compared with the negative fraction. CD146+cells expressed the immunophenotypic characteristics of pericytes. With prolonged in vitro expansion, CD146-cells exhibited increased population doubling times and morphological signs of senescence, whereas CD146+cells did not. CD146+cells expressed higher levels of the angiogenic molecules VEGF-A, angiopoietin-1 and FGF-1. Conditioned medium taken from CD146+cells significantly increased formation of in vitro endothelial cell tube networks, whereas CD146-cells did not. CD146+cells could be differentiated into adipocytes in greater numbers than CD146-cells. Consistent with this, differentiated CD146+cells expressed higher levels of the adipocyte markers adiponectin and leptin. These results suggest that CD146+cells selected from a heterogeneous mix of ASCs have more favourable angiogenic and adipogenic properties, which might provide significant benefits for reconstructive and tissue-engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Integrative Medical Biology, Section for Anatomy, Umeå University, Sweden.|Department of Surgical and Perioperative Sciences, Section for Hand and Plastic Surgery, Umeå University, Sweden.
authors.forename: Anne Therese
authors.initials: AT
authors.lastname: Lauvrud
authors.affiliation: Department of Integrative Medical Biology, Section for Anatomy, Umeå University, Sweden.
authors.forename: Peyman
authors.initials: P
authors.lastname: Kelk
authors.affiliation: Department of Integrative Medical Biology, Section for Anatomy, Umeå University, Sweden.|Department of Surgical and Perioperative Sciences, Section for Hand and Plastic Surgery, Umeå University, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Wiberg
authors.affiliation: Department of Integrative Medical Biology, Section for Anatomy, Umeå University, Sweden.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kingham
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation; D002469:Cell Separation; D006801:Humans; D018919:Neovascularization, Physiologic; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation
keywords: Adipogenesis; adult stem cell; angiogenesis; fat grafting; pericyte; regeneration