title: [Analysis of the association of human leukocyte antigen DQ gene polymorphisms with unexplained recurrent spontaneous abortion among ethnic Han Chinese from Wenzhou region].
abstract: OBJECTIVE
  To assess the association of human leukocyte antigen DQ gene polymorphisms with unexplained recurrent spontaneous abortion (URSA) among ethnic Han Chinese from Wenzhou region.
  
  
  METHODS
  Fifty couples with URSA (URSA group) and 66 couples with normal pregnancy history (control group) were recruited. The alleles of HLA-DQA1 and HLA-DQB1 were analyzed by polymerase chain reaction with specific sequence primers (PCR-SSP) in all subjects. The frequency distribution of HLA-DQ alleles, odds ratios (OR) between each group and sharing of HLA-DQ alleles were calculated.
  
  
  RESULTS
  The frequency distribution of HLA-DQB1*03:03 allele in the females with URSA was significantly higher than that healthy females (21.00% vs. 9.85%, OR=2.433, 95%CI: 1.232-4.894, χ(2)=5.657, P<0.05). The HLA-DQB1*05:03 allele was present among the healthy females with a frequency of 3.03%, and was not detected among females with URSA. For both males and females, the HLA-DQB1*05:02 allele were only typed in control group with frequencies of 6.06% and 5.30%, respectively. The sharing of HLA-DQA1 alleles in couples with URSA was increased compared with the control group (70.27% vs. 44.64%, OR=2.931, 95%CI: 1.216-7.067, P<0.05).
  
  
  CONCLUSIONS
  The increased sharing of HLA-DQA1 alleles may contribute to the susceptibility of URSA among ethnic Han Chinese from Wenzhou region. The allele of HLA-DQB1*03:03 in the females may be predisposing factor for URSA. However, the HLA-DQB1*05:02 allele in both gender and HLA-DQB1*05:03 allele in females may confer a protective effect.
authors.affiliation: Department of Gynecology and Obstetrics, Wenzhou People's Hospital, Wenzhou Maternal and Child Health Care Hospital, Wenzhou City Key Laboratory of Gynecology and Obstetrics, Wenzhou, Zhejiang 325000, China. wryzheng@126.com.
authors.forename: Jiayong
authors.initials: J
authors.lastname: Zheng
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Xu
authors.forename: Weide
authors.initials: W
authors.lastname: Ma
authors.forename: Jianxin
authors.initials: J
authors.lastname: Li
authors.forename: Shuqi
authors.initials: S
authors.lastname: Xia
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Shen
mesh_terms: D000022:Abortion, Spontaneous / Q000208:ethnology / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics; D059848:HLA-DQ alpha-Chains / Q000235:genetics*; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D011247:Pregnancy
chemical_list: D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen