title: In Situ Hybridization of Breast Cancer Markers.
abstract: In situ hybridization is an important technique in breast cancer research, which is widely applied in detection of specific nucleic acid sequences. Here, we describe the detailed protocol of fluorescence in situ hybridization and chromogenic in situ hybridization in detection of gene HER2/neu amplification in breast cancer tissues.
authors.affiliation: Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, 52# Fu-Cheng Road, Haidian District, Beijing, 100142, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Min
authors.affiliation: Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, 52# Fu-Cheng Road, Haidian District, Beijing, 100142, People's Republic of China. cshou@vip.sina.com.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Shou
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: Breast cancer; CISH; FISH; HER2; In situ hybridization