title: Sulfated polysaccharide isolated from the sea cucumber Stichopus japonicas promotes the SDF-1α/CXCR4 axis-induced NSC migration via the PI3K/Akt/FOXO3a, ERK/MAPK, and NF-κB signaling pathways.
abstract: The present study describes a positive regulatory loop between SJP and the SDF-1α/CXCR4 axis in NSC migration. The treatment of NSCs with SJP and SDF-1α increases the cell migration capacity and promotes cell migration from the neurospheres. These effects are accompanied by the up-regulation of Nestin, N-cadherin, TLR4, TNF-α, Cyclin D1, EGFR, Alpha 6 integrin, MMP-2, MMP-9, and iNOS, including SDF-1α and CXCR4 themselves. However, these effects are blocked by AMD3100, LY294002, U0126, and PDTC. SJP enhances the SDF-1α/CXCR4 axis-induced MMP-2 and MMP-9 secretion and NO release. Results demonstrate that interaction of SJP with the SDF-1α/CXCR4 axis regulates NSC migration via the PI3K/Akt/FOXO3a, ERK-MAPK, and NF-κB signaling pathways.
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China; School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Cui
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Ningshan
authors.initials: N
authors.lastname: Cui
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Song
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China; School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China. Electronic address: jiaiguo@sdu.edu.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Ji
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D039503:Integrin alpha6 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D017208:Rats, Wistar; D019718:Receptors, CXCR4 / Q000378:metabolism*; D012616:Sea Cucumbers / Q000737:chemistry*; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015820:Cadherins; D054377:Chemokine CXCL12; D051858:Forkhead Transcription Factors; D039503:Integrin alpha6; D016328:NF-kappa B; D064231:Nestin; D011134:Polysaccharides; D019718:Receptors, CXCR4; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D019938:Cyclin D1; D009569:Nitric Oxide; D019869:Phosphatidylinositol 3-Kinases; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Migration; NSC; SDF-1α; SJP