title: Assessing early fitness consequences of exotic gene flow in the wild: a field study with Iberian pine relicts.
abstract: Gene flow from plantations of nonlocal (genetically exotic) tree provenances into natural stands of the same species is probably a widespread phenomenon, but its effects remain largely unexamined. We investigated early fitness consequences of intraspecific exotic gene flow in the wild by assessing differences in survival among native, nonlocal, and F1 intraspecific hybrid seedlings naturally established within two native pine relicts (one of Pinus pinaster and the other of P. sylvestris) surrounded by nonlocal plantations. We obtained broad-scale temporally sequential genotypic samples of a cohort of recruits in each pine relict, from seeds before dispersal to established seedlings months after emergence, tracking temporal changes in the estimated proportion of each parental cross-type. Results show significant proportions of exotic male gametes before seed dispersal in the two pine relicts. Subsequently to seedling establishment, the frequency of exotic male gametes became nonsignificant in P. pinaster, and dropped by half in P. sylvestris. Exotic zygotic gene flow was significantly different from zero among early recruits for P. sylvestris, decreasing throughout seedling establishment. Seedling mortality resulted in small late sample sizes, and temporal differences in exotic gene flow estimates were not significant, so we could not reject the null hypothesis of invariant early viability across parental cross types in the wild.
authors.affiliation: Department of Forest Ecology & Genetics INIA-CIFOR Madrid Spain.
authors.forename: Gregor M
authors.initials: GM
authors.lastname: Unger
authors.affiliation: Department of Forest Ecology & Genetics INIA-CIFOR Madrid Spain; INRA UMR 1202 BIOGECO Cestas France; University of Bordeaux UMR 1202 BIOGECO Talence France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Heuertz
authors.affiliation: Institute of Biosciences and Bioresources National Research Council Sesto Fiorentino (FI) Italy.
authors.forename: Giovanni G
authors.initials: GG
authors.lastname: Vendramin
authors.affiliation: Department of Forest Ecology & Genetics INIA-CIFOR Madrid Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Robledo-Arnuncio
keywords: Pinus pinaster; Pinus sylvestris; early fitness; gene flow; introgression; plantations; pollen dispersal; seed dispersal
references.citation: Theor Popul Biol. 1999 Jun;55(3):283-96
references.pmid: 10366553
references.citation: Genetics. 2000 Jun;155(2):945-59
references.pmid: 10835412
references.citation: Trends Ecol Evol. 2001 Feb 1;16(2):62-63
references.pmid: 11165697
references.citation: Genetics. 2002 Mar;160(3):1217-29
references.pmid: 11901135
references.citation: Genetics. 2003 Mar;163(3):1177-91
references.pmid: 12663554
references.citation: Mol Ecol. 2003 Nov;12(11):3157-64
references.pmid: 14629394
references.citation: Proc Biol Sci. 2005 Jul 7;272(1570):1333-8
references.pmid: 16006327
references.citation: Biometrics. 1992 Jun;48(2):361-72
references.pmid: 1637966
references.citation: J Evol Biol. 2006 Jan;19(1):203-15
references.pmid: 16405592
references.citation: Trends Ecol Evol. 2004 Sep;19(9):489-96
references.pmid: 16701312
references.citation: Trends Ecol Evol. 2005 Mar;20(3):136-42
references.pmid: 16701357
references.citation: Proc Biol Sci. 2007 Sep 22;274(1623):2357-64
references.pmid: 17623641
references.citation: J Evol Biol. 2008 Jan;21(1):294-309
references.pmid: 17995948
references.citation: Mol Ecol. 2008 Jan;17(1):314-24
references.pmid: 18173503
references.citation: Genetics. 2008 Mar;178(3):1491-504
references.pmid: 18245344
references.citation: J Hered. 2009 Jan-Feb;100(1):106-13
references.pmid: 18936113
references.citation: Mol Ecol. 2009 Mar;18(6):1048-60
references.pmid: 19222755
references.citation: Mol Ecol. 2008 Sep;17(18):4015-26
references.pmid: 19238703
references.citation: Heredity (Edinb). 2009 Nov;103(5):385-93
references.pmid: 19603058
references.citation: Proc Biol Sci. 2009 Oct 22;276(1673):3601-10
references.pmid: 19640880
references.citation: Proc Natl Acad Sci U S A. 2009 Nov 17;106 Suppl 2:19685-92
references.pmid: 19805104
references.citation: Mol Ecol. 2010 May;19(9):1787-800
references.pmid: 20345684
references.citation: Trends Ecol Evol. 2010 Sep;25(9):520-9
references.pmid: 20688414
references.citation: Mol Ecol. 2010 Sep;19(18):3845-52
references.pmid: 20735737
references.citation: Heredity (Edinb). 2011 Mar;106(3):500-10
references.pmid: 21224876
references.citation: Mol Ecol Resour. 2010 May;10(3):564-7
references.pmid: 21565059
references.citation: Mol Ecol Resour. 2012 Mar;12(2):299-311
references.pmid: 22085307
references.citation: Mol Ecol. 2012 Jan;21(2):300-13
references.pmid: 22151559
references.citation: Ecol Lett. 2012 Mar;15(3):251-9
references.pmid: 22248112
references.citation: Ecol Lett. 2012 Apr;15(4):378-92
references.pmid: 22372546
references.citation: Proc Biol Sci. 2013 Jan 7;280(1750):20122058
references.pmid: 23173202
references.citation: Ecol Lett. 2014 Mar;17(3):369-79
references.pmid: 24354456
references.citation: Mol Ecol. 2014 Nov;23(22):5435-47
references.pmid: 25277767
references.citation: PLoS One. 2014 Oct 06;9(10):e109132
references.pmid: 25286410
references.citation: Trends Ecol Evol. 2015 Jan;30(1):42-9
references.pmid: 25435267
references.citation: Tree Physiol. 2014 Dec;34(12):1376-87
references.pmid: 25466514
references.citation: Proc Biol Sci. 2015 Mar 07;282(1802):null
references.pmid: 25608883
references.citation: Evolution. 1983 Nov;37(6):1210-1226
references.pmid: 28556011
references.citation: Evolution. 1992 Jun;46(3):616-626
references.pmid: 28568666
references.citation: Evolution. 1997 Feb;51(1):21-28
references.pmid: 28568782