title: The "Liquid Biopsy": the Role of Circulating DNA and RNA in Central Nervous System Tumors.
abstract: The detection of tumor-derived circulating nucleic acids in patients with cancer, known as the "liquid biopsy," has expanded from use in plasma to other bodily fluids in an increasing number of malignancies. Circulating nucleic acids could be of particular use in central nervous system tumors as biopsy carries a 5-7 % risk of major morbidity. This application presents unique challenges that have limited the use of cell-free DNA and RNA in the diagnosis and monitoring of CNS tumors. Recent work suggests that cerebrospinal fluid may be a useful source of CNS tumor-derived circulating nucleic acids. In this review, we discuss the available data and future outlook on the use of the liquid biopsy for CNS tumors.
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA. iancon16@stanford.edu.|Stanford University Medical Center, 1201 Welch Rd, MSLS P151, Stanford, CA, 94305, USA. iancon16@stanford.edu.|Stanford University School of Medicine, 291 Campus Drive, Stanford, 94305, CA, USA. iancon16@stanford.edu.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Connolly
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA. yingmeil@stanford.edu.|Stanford University Medical Center, 1201 Welch Rd, MSLS P151, Stanford, CA, 94305, USA. yingmeil@stanford.edu.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA. mghayden@stanford.edu.|Stanford University Medical Center, 1205 Welch Rd, MSLS R307, Stanford, CA, 94305, USA. mghayden@stanford.edu.
authors.forename: Melanie Hayden
authors.initials: MH
authors.lastname: Gephart
authors.affiliation: Department of Neurology, Division of Neuro-oncology, Stanford University School of Medicine, Stanford, CA, USA. snagpal@stanford.edu.|Stanford University Medical Center, Cancer Center, 875 Blake Wilbur Dr, CC2221, Stanford, CA, 94305, USA. snagpal@stanford.edu.
authors.forename: Seema
authors.initials: S
authors.lastname: Nagpal
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002474:Cell-Free System; D016543:Central Nervous System Neoplasms / Q000737:chemistry* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D012313:RNA / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm; D012313:RNA
keywords: Brain tumor; Circulating tumor cells; Liquid biopsy; cfDNA; miRNA
references.citation: Cancer Res. 2014 Apr 15;74(8):2152-9
references.pmid: 24525740
references.citation: Mol Neurobiol. 2015;51(3):1452-61
references.pmid: 25081587
references.citation: World J Surg. 2004 Jul;28(7):721-6
references.pmid: 15185002
references.citation: Clin Cancer Res. 2009 Feb 1;15(3):1032-8
references.pmid: 19188176
references.citation: J Clin Oncol. 2008 Jul 1;26(19):3213-21
references.pmid: 18591556
references.citation: J Clin Oncol. 2015 Apr 20;33(12):1348-55
references.pmid: 25800753
references.citation: Neuro Oncol. 2010 Jun;12(6):540-8
references.pmid: 20154338
references.citation: J Clin Oncol. 2010 Apr 1;28(10):1721-6
references.pmid: 20194856
references.citation: Clin Chem. 2013 Jan;59(1):110-8
references.pmid: 23014601
references.citation: N Engl J Med. 2004 Aug 19;351(8):781-91
references.pmid: 15317891
references.citation: Cancer Genomics Proteomics. 2009 Sep-Oct;6(5):281-95
references.pmid: 19996134
references.citation: J Neurol Sci. 2015 Jan 15;348(1-2):181-7
references.pmid: 25510379
references.citation: Lancet. 1990 Feb 24;335(8687):440-1
references.pmid: 1968171
references.citation: RNA. 2013 May;19(5):712-22
references.pmid: 23525801
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16368-73
references.pmid: 16258065
references.citation: Neuro Oncol. 2010 Feb;12(2):173-80
references.pmid: 20150384
references.citation: J Infect Dis. 1995 Apr;171(4):857-63
references.pmid: 7706811
references.citation: Clin Chem. 2004 Mar;50(3):564-73
references.pmid: 14718398
references.citation: Cancer Discov. 2015 Nov;5(11):1164-1177
references.pmid: 26410082
references.citation: Obstet Gynecol. 2015 Sep;126(3):691-692
references.pmid: 26287784
references.citation: Nat Rev Cancer. 2011 Jun;11(6):426-37
references.pmid: 21562580
references.citation: Clin Cancer Res. 2000 Mar;6(3):1119-24
references.pmid: 10741742
references.citation: J Dent Res. 2004 Mar;83(3):199-203
references.pmid: 14981119
references.citation: Nat Rev Clin Oncol. 2013 Aug;10(8):472-84
references.pmid: 23836314
references.citation: Clin Cancer Res. 2003 Apr;9(4):1461-8
references.pmid: 12684420
references.citation: Cancer Discov. 2014 Nov;4(11):1299-309
references.pmid: 25139148
references.citation: Cancer Discov. 2014 Jun;4(6):650-61
references.pmid: 24801577
references.citation: N Engl J Med. 2012 Mar 8;366(10):883-892
references.pmid: 22397650
references.citation: Cancer Res. 1977 Mar;37(3):646-50
references.pmid: 837366
references.citation: Cancer Lett. 2008 May 18;263(2):312-20
references.pmid: 18280643
references.citation: Clin Cancer Res. 2000 Oct;6(10):3823-6
references.pmid: 11051224
references.citation: J Cell Mol Med. 2015 Oct;19(10):2307-23
references.pmid: 26119132
references.citation: Clin Chem. 2015 Mar;61(3):514-22
references.pmid: 25605683
references.citation: Br J Haematol. 2008 May;141(5):672-5
references.pmid: 18318758
references.citation: Mol Ther Nucleic Acids. 2013 Jul 23;2:e109
references.pmid: 23881452
references.citation: Res Rep Urol. 2012 Jun 27;4:33-40
references.pmid: 24199178
references.citation: Nat Med. 2014 May;20(5):548-54
references.pmid: 24705333
references.citation: Neuro Oncol. 2012 Jun;14(6):689-700
references.pmid: 22492962
references.citation: Clin Chem. 1999 Aug;45(8 Pt 1):1292-4
references.pmid: 10430801
references.citation: Sci Transl Med. 2014 Feb 19;6(224):224ra24
references.pmid: 24553385
references.citation: Sci Transl Med. 2014 Jul 30;6(247):247ra101
references.pmid: 25080476
references.citation: Cancer Res. 2003 Jul 15;63(14):3866-71
references.pmid: 12873974
references.citation: C R Seances Soc Biol Fil. 1948 Feb;142(3-4):241-3
references.pmid: 18875018
references.citation: Anal Biochem. 2013 Feb 15;433(2):227-34
references.pmid: 22750103
references.citation: Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9704-9
references.pmid: 26195750
references.citation: Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10513-8
references.pmid: 18663219
references.citation: Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4589-94
references.pmid: 9539782
references.citation: Neuro Oncol. 2012 Jan;14(1):29-33
references.pmid: 21937590
references.citation: Ann N Y Acad Sci. 2008 Aug;1137:18-26
references.pmid: 18837919
references.citation: Immunology. 2005 May;115(1):55-62
references.pmid: 15819697
references.citation: Clin Cancer Res. 1999 Aug;5(8):1961-5
references.pmid: 10473072
references.citation: PLoS One. 2008;3(11):e3694
references.pmid: 19002258
references.citation: J Clin Microbiol. 1991 Jul;29(7):1513-6
references.pmid: 1653267
references.citation: Nat Rev Clin Oncol. 2013 Jul;10(7):377-89
references.pmid: 23712187
references.citation: Blood. 2011 Mar 17;117(11):3140-6
references.pmid: 21200023
references.citation: Ann N Y Acad Sci. 2001 Sep;945:179-88
references.pmid: 11708476