title: Photoperiod regulates growth of male accessory glands through juvenile hormone signaling in the linden bug, Pyrrhocoris apterus.
abstract: Adult reproductive diapause is characterized by lower behavioral activity, ceased reproduction and absence of juvenile hormone (JH). The role of JH receptor Methoprene-tolerant (Met) in female reproduction is well established; however, its function in male reproductive development and behavior is unclear. In the bean bug, Riptortus pedestris, circadian genes are essential for mediating photoperiodically-dependent growth of the male accessory glands (MAGs). The present study explores the role of circadian genes and JH receptor in male diapause in the linden bug, Pyrrhocoris apterus. These data indicate that circadian factors Clock, Cycle and Cry2 are responsible for photoperiod measurement, whereas Met and its partner protein Taiman participate in JH reception. Surprisingly, knockdown of the JH receptor neither lowered locomotor activity nor reduced mating behavior of males. These data suggest existence of a parallel, JH-independent or JH-upstream photoperiodic regulation of reproductive behavior.
authors.affiliation: Institute of Entomology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Urbanová
authors.affiliation: Institute of Entomology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Sciences, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Bazalová
authors.affiliation: Institute of Entomology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic.
authors.forename: Hanka
authors.initials: H
authors.lastname: Vaněčková
authors.affiliation: Institute of Entomology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Sciences, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic. Electronic address: david.dolezel@entu.cas.cz.
authors.forename: David
authors.initials: D
authors.lastname: Dolezel
mesh_terms: D000818:Animals; D020062:Heteroptera / Q000254:growth & development* / Q000502:physiology; D007605:Juvenile Hormones / Q000378:metabolism*; D008124:Locomotion; D008297:Male; D008726:Methoprene / Q000378:metabolism; D017440:Photoperiod; D012726:Sexual Behavior, Animal; D015398:Signal Transduction
chemical_list: D007605:Juvenile Hormones; D008726:Methoprene
keywords: Circadian genes; Diapause; Locomotor activity; Mating behavior; Methoprene-tolerant; bHLH-PAS