title: Quantitative proteomics analysis reveals glutamine deprivation activates fatty acid β-oxidation pathway in HepG2 cells.
abstract: Glutamine, a multifunctional amino acid, functions in nutrient metabolism, energy balance, apoptosis, and cell proliferation. Lipid is an important nutrient and controls a broad range of physiological processes. Previous studies have demonstrated that glutamine can affect lipolysis and lipogenesis, but the effect of glutamine on the detailed lipid metabolism remains incompletely understood. Here, we applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. The results showed that there were 212 differentially abundant proteins in response to glutamine deprivation, including 150 significantly increased proteins and 62 significantly decreased proteins. Interestingly, functional classification showed that 43 differentially abundant proteins were related to lipid metabolism. Further bioinformatics analysis and western blotting validation revealed that lipid accumulation may be affected by β-oxidation of fatty acid induced by glutamine deprivation in HepG2 cells. Together, our results may provide the potential for regulating lipid metabolism by glutamine in animal production and human nutrition. The MS data have been deposited to the ProteomeXchange Consortium with identifier PXD003387.
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors.forename: Baisheng
authors.initials: B
authors.lastname: Long
authors.affiliation: Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, 50275, Central Java, Indonesia.
authors.forename: Rodiallah
authors.initials: R
authors.lastname: Muhamad
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors.forename: Guokai
authors.initials: G
authors.lastname: Yan
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Fan
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors.forename: Zhichang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors.forename: Xiuzhi
authors.initials: X
authors.lastname: Li
authors.affiliation: Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, 50275, Central Java, Indonesia.
authors.forename: Agung
authors.initials: A
authors.lastname: Purnomoadi
authors.affiliation: Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, 50275, Central Java, Indonesia.
authors.forename: Joelal
authors.initials: J
authors.lastname: Achmadi
authors.affiliation: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. xhyan@mail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China. xhyan@mail.hzau.edu.cn.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Yan
mesh_terms: D005227:Fatty Acids / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D005227:Fatty Acids; D011506:Proteins; D005973:Glutamine
keywords: Fatty acid; Glutamine; HepG2 cells; Lipid metabolism; Quantitative proteomics