title: Relationship of Grafted FGF-2-Overexpressing Neural Stem/Progenitor Cells With the Vasculature in the Cerebral Cortex.
abstract: Neural progenitor cells (NPCs) overexpressing fibroblast growth factor 2 (FGF-2) have the distinct tendency to associate with the vasculature and establish multiple proliferative clusters in the perivascular environment after transplantation into the cerebral cortex. Strikingly, the vascular clusters of progenitor cells give rise to immature neurons after ischemic injury, raising prospects for the formation of ectopic neurogenic niches for repair. We investigated the spatial relationship of perivascular clusters with the host vascular structures. FGF-2-GFP-transduced NPCs were transplanted into the intact somatosensory rat cortex. Confocal microscopic analysis revealed that grafted cells preferentially contacted venules at sites with aquaporin-4-positive astrocytic endfeet and avoided contacts with desmin-positive pericytes. Electron microscopic analysis confirmed that grafted cells preferentially made contact with astroglial endfeet, and only a minority of them reached the endothelial basal lamina. These results provide new insights into the fine structural and anatomical relationship between grafted FGF-2-transduced NPCs and the host vasculature.
authors.affiliation: Department of Cytology, Bogomoletz Institute of Physiology, Kyiv, Ukraine.
authors.forename: Oleg
authors.initials: O
authors.lastname: Tsupykov
authors.forename: Michiko
authors.initials: M
authors.lastname: Kanemitsu
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Smozhanik
authors.forename: Galina
authors.initials: G
authors.lastname: Skibo
authors.forename: Alexandre G
authors.initials: AG
authors.lastname: Dayer
authors.forename: Jozsef Z
authors.initials: JZ
authors.lastname: Kiss
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology; D001808:Blood Vessels / Q000166:cytology / Q000648:ultrastructure; D002449:Cell Aggregation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply* / Q000166:cytology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D058953:Neural Stem Cells / Q000378:metabolism* / Q000637:transplantation*; D020286:Pericytes / Q000166:cytology; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000166:cytology
chemical_list: D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins