title: Wolbachia in European Populations of the Invasive Pest Drosophila suzukii: Regional Variation in Infection Frequencies.
abstract: The invasive pest Drosophila suzukii is characterized by a specific fresh-fruit targeting behavior and has quickly become a menace for the fruit economy of newly infested North American and European regions. D. suzukii carries a strain of the endosymbiotic bacterium Wolbachia, named wSuz, which has a low infection frequency and no reproductive manipulation capabilities in American populations of D. suzukii. To further understand the nature of wSuz biology and assess its utility as a tool for controlling this pest's populations, we investigated the prevalence of Wolbachia in 23 European D. suzukii populations, and compared our results with those available in American populations. Our data showed a highly variable infection frequency with a mean prevalence of 46%, which is significantly higher than the 17% found in American populations. Based on Multilocus Sequence Typing analysis, a single wSuz strain was diagnosed in all European populations of D. suzukii. In agreement with American data, we found no evidence of cytoplasmic incompatibility induced by wSuz. These findings raise two questions: a) why Wolbachia is maintained in field populations of D. suzukii and b) what are the selective forces responsible for the variation in prevalence within populations, particularly between European and American continents? Our results provide new insights into the D. suzukii-Wolbachia association and highlight regional variations that await further investigation and that should be taken into account for using Wolbachia-based pest management programs.
authors.affiliation: Université de Lyon, Université Lyon1, Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Cattel
authors.affiliation: Chemical Ecology lab, Department of Sustainable Agro-ecosystems and Bio-resources, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy.|Laboratory of Genome Dynamics, Department of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.
authors.forename: Rupinder
authors.initials: R
authors.lastname: Kaur
authors.affiliation: Université de Lyon, Université Lyon1, Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gibert
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Julien
authors.initials: J
authors.lastname: Martinez
authors.affiliation: Institut de Systématique, Évolution, Biodiversité ISYEB - UMR 7205 - CNRS, MNHN, UPMC, EPHE Muséum national d'Histoire naturelle, Sorbonne Universités57 rue Cuvier, CP50F-75005, Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Fraimout
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Francis
authors.initials: F
authors.lastname: Jiggins
authors.affiliation: Université de Lyon, Université Lyon1, Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Andrieux
authors.affiliation: Chemical Ecology lab, Department of Sustainable Agro-ecosystems and Bio-resources, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy.|Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Stefanos
authors.initials: S
authors.lastname: Siozios
authors.affiliation: Chemical Ecology lab, Department of Sustainable Agro-ecosystems and Bio-resources, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Anfora
authors.affiliation: Laboratory of Genome Dynamics, Department of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Miller
authors.affiliation: Chemical Ecology lab, Department of Sustainable Agro-ecosystems and Bio-resources, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy.
authors.forename: Omar
authors.initials: O
authors.lastname: Rota-Stabelli
authors.affiliation: Université de Lyon, Université Lyon1, Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Mouton
mesh_terms: D000818:Animals; D004330:Drosophila / Q000382:microbiology*; D005060:Europe; D058885:Multilocus Sequence Typing; D009656:North America; D020577:Wolbachia / Q000145:classification / Q000235:genetics / Q000502:physiology*
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