title: Green synthesis of silver nanoparticles and characterization of their inhibitory effects on AGEs formation using biophysical techniques.
abstract: Advanced glycation end-products (AGEs) resulting from non-enzymatic glycation are one of the major factors implicated in secondary complications of diabetes. Scientists are focusing on discovering new compounds that may be used as potential AGEs inhibitors without affecting the normal structure and function of biomolecules. A number of natural and synthetic compounds have been proposed as AGE inhibitors. In this study, we investigated the inhibitory effects of AgNPs (silver nanoparticles) in AGEs formation. AgNPs (~30.5 nm) synthesized from Aloe Vera leaf extract were characterized using UV-Vis spectroscopy, energy-dispersive X-ray spectroscopy (EDX), high resolution-transmission electron microscopy, X-ray diffraction and dynamic light scattering (DLS) techniques. The inhibitory effects of AgNPs on AGEs formation were evaluated by investigating the degree of reactivity of free amino groups (lysine and arginine residues), protein-bound carbonyl and carboxymethyl lysine (CML) content, and the effects on protein structure using various physicochemical techniques. The results showed that AgNPs significantly inhibit AGEs formation in a concentration dependent manner and that AgNPs have a positive effect on protein structure. These findings strongly suggest that AgNPs may play a therapeutic role in diabetes-related complications.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Jalaluddin M
authors.initials: JM
authors.lastname: Ashraf
authors.affiliation: Nanotechnology and Antimicrobial Drug Resistance Research Laboratory, Department of Microbiology, Jawaharlal Nehru Medical College &Hospital, Aligarh Muslim University, Aligarh-202002, U.P., India.|Department of Medical Laboratories, College of Applied Medical Science, Buraydah Colleges, Buraydah 51452, Saudi Arabia.
authors.forename: Mohammad Azam
authors.initials: MA
authors.lastname: Ansari
authors.affiliation: Nanotechnology and Antimicrobial Drug Resistance Research Laboratory, Department of Microbiology, Jawaharlal Nehru Medical College &Hospital, Aligarh Muslim University, Aligarh-202002, U.P., India.
authors.forename: Haris M
authors.initials: HM
authors.lastname: Khan
authors.affiliation: Department of Medical Laboratories, College of Applied Medical Science, Buraydah Colleges, Buraydah 51452, Saudi Arabia.|Department of Medical Laboratories, College of Medical Science, Qassim University, Saudi Arabia.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Alzohairy
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D017127:Glycation End Products, Advanced; D012834:Silver
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