title: [Effect of 10-hydroxycamptotbecine on the expression of VEGF in rheumatoid arthritis synovial fibroblasts].
abstract: OBJECTIVE
  To study the effect of 10-Hydroxycamptotbecine (10-HCPT) on the expression of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblasts (RASFs).
  
  
  METHODS
  RASFs were isolated form synovial tissue of RA patients and cultured in vitro. 10-HCPT (3.0×10(-6) mol/L, 3.0×10(-5) mol/L) and Methotrexate (MTX) (2.0×10(-6) mol/L, 2.0×10(-5) mol/L) with different concentrations were used to treat RASFs for 48 hours, and RASFs without 10-HCPT and MTX treatment were served as the control group. The level of VEGF in the supernatant of RASFs culture was measured by enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Compared with control group, high and low dose 10-HCPT groups showed significant values on the effect of inhibiting the expression of VEGF in RASFs. Compared with low-dose MTX group, both high and low dose 10-HCPT groups showed significant difference (P<0.05); compared with high-dose MTX group, the high-dose 10-HCPT group had significant difference (P<0.05).
  
  
  CONCLUSIONS
  Compared with MTX, 10-HCPT showed significant effect on inhibiting the expression of VEGF in RASFs.
authors.affiliation: Department of Rheumatology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ren
authors.affiliation: Email: wangyanh_1963@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Sun
authors.forename: Shaoting
authors.initials: S
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
mesh_terms: D001172:Arthritis, Rheumatoid; D002166:Camptothecin / Q000031:analogs & derivatives; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D006801:Humans; D008727:Methotrexate; D013583:Synovial Membrane; D042461:Vascular Endothelial Growth Factor A
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C028098:10-hydroxycamptothecin; D002166:Camptothecin; D008727:Methotrexate