title: Zinc finger protein x-linked (ZFX) contributes to patient prognosis, cell proliferation and apoptosis in human laryngeal squamous cell carcinoma.
abstract: Zinc finger protein, X-linked (ZFX) gene locus on the human X chromosome is structurally similar to the zinc finger protein, Y-linked gene. Its role in human laryngeal squamous cell carcinoma (LSCC) is still not clearly defined. This study was focused on investigating the role of zinc-finger protein X-linked (ZFX) in human LSCC. Expression levels of ZFX were examined in LSCC tissues, corresponding adjacent non-tumoral tissues and vocal leukoplakia tissues by immunohistochemistry (IHC). The association with the expression level of ZFX and LSCC clincopathological parameters was analyzed. The prognostic value of ZFX expression was also analyzed. Lentivirus-mediated RNA interference was applied to silence ZFX expression and the effects of ZFX knockdown on the growth of human LSCC primary cells was investigated. Overexpression of ZFX was found in LSCC tissues. The expression of ZFX was associated with the clinical stage of LSCC. Patients with higher level of ZFX experienced a poorer prognosis compared to those with lower level of ZFX. Knockdown of ZFX inhibited cell proliferation, colony formation and migration of LSCC primary cells. Moreover, ZFX silencing induced cell apoptosis. These results provide the convincing evidence for the first time that ZFX plays an important role in LSCC development and could be a potential therapeutic target or prognostic predictor for LSCC.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University Beijing 100029, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityBeijing 100730, China; Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical UniversityBeijing 100730, China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityBeijing 100730, China; Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical UniversityBeijing 100730, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University Beijing 100730, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Lian
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University Beijing 100730, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical University Beijing 100730, China.
authors.forename: Erzhong
authors.initials: E
authors.lastname: Fan
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityBeijing 100730, China; Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical UniversityBeijing 100730, China; Beijing Key Laboratory of Head and Neck Molecular Diagnostic PathologyBeijing 100730, China.
authors.forename: Jugao
authors.initials: J
authors.lastname: Fang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D006801:Humans; D053208:Kaplan-Meier Estimate; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D034622:RNA Interference; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck; D013997:Time Factors; D014162:Transfection; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: D051741:Kruppel-Like Transcription Factors; C062076:zinc finger protein, X-linked
keywords: Laryngeal squamous cell carcinoma (LSCC); apoptosis; prognosis; proliferation; zinc finger protein x-linked (ZFX)
references.citation: Int J Clin Exp Pathol. 2014;7(6):3150-7
references.pmid: 25031734
references.citation: Cancer Chemother Pharmacol. 2014 Mar;73(3):569-76
references.pmid: 24448637
references.citation: Int J Cancer. 2014 Oct 15;135(8):1790-9
references.pmid: 24585547
references.citation: J Exp Clin Cancer Res. 2011;30:114
references.pmid: 22185393
references.citation: Laryngoscope. 2012 Feb;122(2):336-42
references.pmid: 22252855
references.citation: J Biosci. 2012 Mar;37(1):85-90
references.pmid: 22357206
references.citation: J Clin Invest. 2012 Mar;122(3):833-43
references.pmid: 22307325
references.citation: J Neurooncol. 2013 Mar;112(1):17-25
references.pmid: 23322077
references.citation: Cancer Lett. 2013 Sep 1;337(2):293-300
references.pmid: 23587796
references.citation: Gene. 2013 Oct 10;528(2):261-6
references.pmid: 23860324
references.citation: Apoptosis. 2007 Sep;12(9):1543-68
references.pmid: 17573556
references.citation: CA Cancer J Clin. 2008 Mar-Apr;58(2):71-96
references.pmid: 18287387
references.citation: Laryngoscope. 2008 Jun;118(6):1003-13
references.pmid: 18388773
references.citation: Otolaryngol Clin North Am. 2008 Aug;41(4):673-95, v
references.pmid: 18570953
references.citation: Drug Resist Updat. 2008 Aug-Oct;11(4-5):123-51
references.pmid: 18718806
references.citation: Genes Dev. 2009 Apr 1;23(7):837-48
references.pmid: 19339689
references.citation: Mol Cell Biochem. 2012 Jan;360(1-2):301-7
references.pmid: 22009483
references.citation: Cancer Genet. 2014 Oct-Dec;207(10-12):461-6
references.pmid: 25441684
references.citation: RNA. 2003 Apr;9(4):493-501
references.pmid: 12649500
references.citation: Nature. 1989 Dec 7;342(6250):708-11
references.pmid: 2512506
references.citation: Laryngoscope. 2006 Sep;116(9 Pt 2 Suppl 111):1-13
references.pmid: 16946667
references.citation: Oncotarget. 2013 Dec;4(12):2512-22
references.pmid: 24318272
references.citation: Int J Clin Exp Pathol. 2013;6(11):2460-7
references.pmid: 24228108
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):973-8
references.pmid: 17210912
references.citation: J Cancer Res Ther. 2013 Nov;9 Suppl:S150-2
references.pmid: 24516051
references.citation: Stem Cells. 2014 Aug;32(8):2033-47
references.pmid: 24831540