title: Changes in intestinal microflora of Caenorhabditis elegans following Bacillus nematocida B16 infection.
abstract: The effect of pathogenic bacteria on a host and its symbiotic microbiota is vital and widespread in the biotic world. The soil-dwelling opportunistic bacterium Bacillus nematocida B16 uses a "Trojan horse" mechanism to kill Caenorhabditis elegans. The alterations in the intestinal microflora that occur after B16 infection remain unknown. Here, we analyzed the intestinal bacteria presented in normal and infected worms. The gut microbial community experienced a complex change after B16 inoculation, as determined through marked differences in species diversity, structure, distribution and composition between uninfected and infected worms. Regardless of the worm's origin (i.e., from soil or rotten fruits), the diversity of the intestinal microbiome decreased after infection. Firmicutes increased sharply, whereas Proteobacteria, Actinobacteria, Cyanobacteria and Acidobacteria decreased to different degrees. Fusobacteria was only present 12 h post-infection. After 24 h of infection, 1228 and 1109 bacterial species were identified in the uninfected and infected groups, respectively. The shared species reached 21.97%. The infected group had a greater number of Bacillus species but a smaller number of Pediococcus, Halomonas, Escherichia and Shewanella species (P < 0.01). Therefore, this study provides the first evaluation of the alterations caused by pathogenic bacteria on symbiotic microbiota using C. elegans as the model species.
authors.affiliation: China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473000, P.R. China.|Laboratory for Conservation and Utilization of Bio-Resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P. R. China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Niu
authors.affiliation: China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473000, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P. R. China.
authors.forename: Keqin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P. R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Huang
authors.affiliation: China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473000, P.R. China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Hui
authors.affiliation: China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473000, P.R. China.
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Kan
authors.affiliation: China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473000, P.R. China.
authors.forename: Lunguang
authors.initials: L
authors.lastname: Yao
mesh_terms: D000818:Animals; D001407:Bacillus / Q000502:physiology*; D001424:Bacterial Infections / Q000382:microbiology*; D017173:Caenorhabditis elegans / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome; D007422:Intestines / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D010802:Phylogeny; D012987:Soil; D013045:Species Specificity
chemical_list: D012987:Soil
references.citation: Postepy Hig Med Dosw (Online). 2013 Apr 15;67:288-303
references.pmid: 23619228
references.citation: J Mol Microbiol Biotechnol. 2012;22(4):258-67
references.pmid: 23037141
references.citation: J Clin Gastroenterol. 2013 Jan;47(1):9-11
references.pmid: 23222210
references.citation: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references.pmid: 17586664
references.citation: Bioinformatics. 2010 Oct 1;26(19):2460-1
references.pmid: 20709691
references.citation: Infect Immun. 2013 Feb;81(2):514-20
references.pmid: 23230286
references.citation: Bioinformatics. 2011 Nov 1;27(21):2957-63
references.pmid: 21903629
references.citation: Nature. 2009 Jan 22;457(7228):480-4
references.pmid: 19043404
references.citation: Appl Environ Microbiol. 2011 May;77(10):3202-10
references.pmid: 21421777
references.citation: Genetics. 1974 May;77(1):71-94
references.pmid: 4366476
references.citation: Nutr Hosp. 2015 Feb 07;31 Suppl 1:3-9
references.pmid: 25659048
references.citation: Cell. 2006 Feb 24;124(4):837-48
references.pmid: 16497592
references.citation: Arch Microbiol. 2006 Jun;185(6):439-48
references.pmid: 16775750
references.citation: PLoS Pathog. 2010 Sep 09;6(9):e1001097
references.pmid: 20844578
references.citation: J Gastrointest Cancer. 2013 Jun;44(2):121-31
references.pmid: 23180023
references.citation: Cell Microbiol. 2008 Jun;10(6):1259-73
references.pmid: 18221392
references.citation: Science. 2001 May 11;292(5519):1115-8
references.pmid: 11352068
references.citation: BMC Biol. 2012 Jun 25;10:59
references.pmid: 22731941
references.citation: Nat Rev Immunol. 2004 Jun;4(6):478-85
references.pmid: 15173836
references.citation: PLoS Pathog. 2010 Jan;6(1):e1000711
references.pmid: 20062525
references.citation: Syst Appl Microbiol. 2005 Jun;28(4):323-7
references.pmid: 15997705
references.citation: ISME J. 2013 Apr;7(4):880-4
references.pmid: 23235292
references.citation: Clin Exp Immunol. 2010 Apr;160(1):48-57
references.pmid: 20415851
references.citation: Appl Microbiol Biotechnol. 2007 May;75(1):141-8
references.pmid: 17458548
references.citation: BMC Biol. 2012 Jul 31;10:67
references.pmid: 22849329
references.citation: Cell. 2014 Dec 4;159(6):1277-89
references.pmid: 25480293
references.citation: World J Gastroenterol. 2014 May 21;20(19):5849-58
references.pmid: 24914345
references.citation: PLoS One. 2013 May 07;8(5):e61628
references.pmid: 23667443
references.citation: BMC Microbiol. 2012 Mar 27;12:49
references.pmid: 22452899
references.citation: PLoS One. 2013 Jul 22;8(7):e67425
references.pmid: 23894287
references.citation: Microbiology (Reading). 1997 Aug;143(8):2593-2604
references.pmid: 33657731
references.citation: Appl Microbiol Biotechnol. 2006 Feb;69(6):722-30
references.pmid: 16025329
references.citation: EMBO Mol Med. 2013 Sep;5(9):1300-10
references.pmid: 23913848
references.citation: Nature. 2007 Oct 18;449(7164):804-10
references.pmid: 17943116
references.citation: Methods Cell Biol. 1995;48:3-29
references.pmid: 8531730
references.citation: Appl Environ Microbiol. 2012 Aug;78(16):5529-35
references.pmid: 22660707
references.citation: BMC Microbiol. 2013 Jan 29;13:19
references.pmid: 23356904
references.citation: Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16631-6
references.pmid: 20733068
references.citation: Nat Methods. 2010 May;7(5):335-6
references.pmid: 20383131
references.citation: Cell. 2012 Aug 3;150(3):470-80
references.pmid: 22863002
references.citation: J Bacteriol. 2013 Feb;195(3):411-6
references.pmid: 23144247
references.citation: Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):3047-52
references.pmid: 21282636