title: Microenvironmental interactions and expression of molecular markers associated with epithelial-to-mesenchymal transition in colorectal carcinoma.
abstract: The tumor microenvironment is known to play a critical role in tumor progression, invasion and metastasis. The epithelial-to-mesenchymal transition (EMT) is understood as a process of tumor invasion and metastasis. Therefore, we investigated the relation between the EMT and the microenvironment of colorectal carcinoma (CRC). The histological features and expression of EMT markers in tumor cells and surrounded stromal cells were obtained from the surgically resected tissues of 39 patients using microscopic review and immunohistochemistry. The loss of expression of E-cadherin was more prominent in the invasive front of tumor than the surface, where α-smooth muscle actin-positive carcinoma-associated fibroblasts (CAFs) are accumulated. The signaling molecules of the Wnt and TGF-β1-Smad pathway were expressed more frequently in the tumor cells and/or CAFs of the invasive margin than those of the tumor surface. The expressions of related transcription factors, such as SNAIL and ZEB1, were increased in the tumor cells and CAFs. The process of EMT may be activated in the tumor margin of CRC under the control of CAFs. Related signaling molecules and transcription factors might be induced by paracrine effects of the surrounding CAFs.
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Sun-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Surgery, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Chun-Seok
authors.initials: CS
authors.lastname: Yang
authors.affiliation: Department of Surgery, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Dae-Dong
authors.initials: DD
authors.lastname: Kim
authors.affiliation: Department of Pathology, Keimyung University School of Medicine Republic of Korea.
authors.forename: Yu-Na
authors.initials: YN
authors.lastname: Kang
authors.affiliation: Department of Medical Statistics, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Kwak
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Jae-Bok
authors.initials: JB
authors.lastname: Park
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Cho
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu Republic of Korea.
authors.forename: Kwan-Kyu
authors.initials: KK
authors.lastname: Park
mesh_terms: D000199:Actins / Q000032:analysis; D000368:Aged; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000032:analysis*; D015820:Cadherins / Q000032:analysis; D002277:Carcinoma / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005347:Fibroblasts / Q000737:chemistry* / Q000473:pathology; D018398:Homeodomain Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D019899:Paracrine Communication; D051785:Smad Proteins / Q000032:analysis; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000032:analysis; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D059016:Tumor Microenvironment; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D018398:Homeodomain Proteins; D051785:Smad Proteins; D000071250:Snail Family Transcription Factors; C508960:TGFB1 protein, human; D014157:Transcription Factors; D053773:Transforming Growth Factor beta1; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1
keywords: Colorectal carcinoma; carcinoma-associated fibroblasts; epithelial-to-mesenchymal transition; microenvironment
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