title: Accumulation of cells expressing macrophage colony-stimulating factor receptor gene in the ovary of a pregnant viviparous fish, Neoditrema ransonnetii (Perciformes, Embiotocidae).
abstract: Macrophage colony-stimulating factor receptor (M-CSFR), a member of the group of type III protein tyrosine kinase receptors, is expressed primarily by monocyte/macrophage lineage cells. In order to describe the distribution of macrophages at the maternal-fetal interface in Neoditrema ransonnetii, a viviparous fish species, M-CSFR cDNA was sequenced. Two sequences were obtained: NrM-CSFR1 (4381 bp, encoding 980 amino acids), and NrM-CSFR2 (3573 bp, encoding 1016 amino acids). Both the genes were expressed in the ovary of pregnant females. In situ hybridization revealed that a number of cells that were positive for NrM-CSFR1 and/or NrM-CSFR2 populated the ovigerous lamellae of the ovary during pregnancy. Following parturition, M-CSFR-positive cells disappeared from the subepithelial region of ovigerous lamellae, and were localized in perivascular tissues. These results suggest the role of M-CSFR-positive cells, which appear to be macrophages, in N. ransonnetii during pregnancy.
authors.affiliation: School of Marine Biosciences, Kitasato University, Kanagawa, 252-0373, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Ueda
authors.affiliation: School of Marine Biosciences, Kitasato University, Kanagawa, 252-0373, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Saito
authors.affiliation: School of Marine Biosciences, Kitasato University, Kanagawa, 252-0373, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Iwasaki
authors.affiliation: School of Marine Biosciences, Kitasato University, Kanagawa, 252-0373, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tsutsui
authors.affiliation: Fisheries Laboratory, the University of Tokyo, Shizuoka, 431-0214, Japan.
authors.forename: Aoi
authors.initials: A
authors.lastname: Nozawa
authors.affiliation: Fisheries Laboratory, the University of Tokyo, Shizuoka, 431-0214, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: School of Marine Biosciences, Kitasato University, Kanagawa, 252-0373, Japan. Electronic address: naosamu@kitasato-u.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D010473:Perciformes; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D052286:Viviparity, Nonmammalian
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D016186:Receptor, Macrophage Colony-Stimulating Factor
keywords: Embiotocidae; Macrophage; Macrophage colony-stimulating factor receptor; Maternal–fetal interface; Neoditrema ransonnetii; Viviparity