title: Small molecule diselenide additives for in vitro oxidative protein folding.
abstract: The in vitro oxidative folding of disulfide-rich proteins can be challenging. Here we show a new class of small molecule diselenides, which can be easily prepared from inexpensive starting materials, used to enhance oxidative protein folding. These compounds were tested on a model protein, bovine pancreatic trypsin inhibitor. Two of the tested diselenides showed considerable improvement over glutathione and were on par with the previously described selenoglutathione.
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. metanis@mail.huji.ac.il.
authors.forename: Post Sai
authors.initials: PS
authors.lastname: Reddy
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. metanis@mail.huji.ac.il.
authors.forename: Norman
authors.initials: N
authors.lastname: Metanis
mesh_terms: D066298:In Vitro Techniques; D007700:Kinetics; D017510:Protein Folding; D012643:Selenium / Q000737:chemistry*
chemical_list: D012643:Selenium