title: Chiral Differentiation of Amino Acids by In-Source Collision-Induced Dissociation Mass Spectrometry.
abstract: Chiral recognition of d- and l-amino acids is achieved by a method combining electrospray ionization (ESI) and in-source collision-induced dissociation (CID) mass spectrometry (MS). Trimeric cluster ions [Cu(II)(A)(ref)2-H](+) are formed by ESI of mixtures of d- or l-analyte amino acid (A), chiral reference (ref) and CuSO4. By increasing the applied voltage in the ESI source region, the trimeric ions become unstable and dissociate progressively. Thus chiral differentiation of the analyte can be achieved by comparing the dependence of their relative intensities to a reference ion on applied voltages. The method does not need MS/MS technique, thus can be readily performed on single-stage MS instruments by turning the voltage of sampling cone.
authors.affiliation: State Key Laboratory and Institute of Elemento-Organic Chemistry, Nankai University.
authors.forename: Xianglei
authors.initials: X
authors.lastname: Kong
authors.affiliation: State Key Laboratory and Institute of Elemento-Organic Chemistry, Nankai University.
authors.forename: Zhaiyi
authors.initials: Z
authors.lastname: Huo
authors.affiliation: State Key Laboratory and Institute of Elemento-Organic Chemistry, Nankai University.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhai
keywords: amino acids; chiral differentiation; cone voltage; electrospray ionization; in-source CID
references.citation: Chem Commun (Camb). 2002 Oct 7;(19):2242-3
references.pmid: 12397997
references.citation: J Am Soc Mass Spectrom. 2006 Aug;17(8):1050-62
references.pmid: 16750381
references.citation: J Am Soc Mass Spectrom. 2006 Mar;17(3):442-52
references.pmid: 16469502
references.citation: J Am Chem Soc. 2005 Aug 31;127(34):11912-3
references.pmid: 16117509
references.citation: Anal Chem. 1999 Oct 1;71(19):4427-9
references.pmid: 21662869
references.citation: J Am Soc Mass Spectrom. 2007 Aug;18(8):1516-24
references.pmid: 17588770
references.citation: Anal Chem. 2003 Jan 1;75(1):25A-31A
references.pmid: 12530808
references.citation: Rapid Commun Mass Spectrom. 2008 May;22(9):1393-8
references.pmid: 18384196
references.citation: Anal Chem. 2005 Sep 15;77(18):5777-84
references.pmid: 16159106
references.citation: Anal Chem. 2009 Sep 15;81(18):7839-43
references.pmid: 19691295
references.citation: J Mass Spectrom. 2011 May;46(5):478-88
references.pmid: 21500306
references.citation: Mass Spectrom Rev. 1997 Mar-Apr;16(2):73-90
references.pmid: 9414491
references.citation: Anal Chem. 2000 Nov 1;72(21):5383-93
references.pmid: 11080891
references.citation: J Am Soc Mass Spectrom. 2003 Jun;14(6):571-80
references.pmid: 12781458
references.citation: Anal Chem. 2003 Feb 1;75(3):678-84
references.pmid: 12585501
references.citation: Rapid Commun Mass Spectrom. 2012 Apr 15;26(7):870-3
references.pmid: 22368068
references.citation: Rapid Commun Mass Spectrom. 2012 Jun 15;26(11):1385-91
references.pmid: 22555933
references.citation: Science. 1989 Oct 6;246(4926):64-71
references.pmid: 2675315
references.citation: Mass Spectrom Rev. 2005 Jul-Aug;24(4):566-87
references.pmid: 15317019