title: TFF3 knockout in human pituitary adenoma cell HP75 facilitates cell apoptosis via mitochondrial pathway.
abstract: Trefoil factor 3 (TFF3), a regulatory protein composed of 59 amino acids, has been suggested to be involved in pathogenesis, proliferation, differentiation, invasion, migration and apoptosis in multiple malignant tumors. This study thus investigated the effect of TFF3 knockout in human pituitary adenoma cell line HP75 on cell apoptosis and related pathways. RNA interference approach was used to knock down the expression of TFF3 protein. The gene silencing was validated by RNA denaturing gel electrophoresis and Western blotting. The effect of TFF3 knockout on cell apoptosis was analyzed by Western blotting and flow cytometry. TFF3 protein level in pituitary adenoma was about 3.61 ± 0.48 folds of that in normal tissues (P < 0.01). After transfecting with small interference RNA (siRNA) against TFF3, the apoptotic ration was significantly elevated (P < 0.01). Apoptosis related protein Bcl-2 and caspase-3 levels were remarkably depressed after siRNA transfection, while Bax and cleaved caspase-3 levels were elevated. TFF3 protein knockout can facilitate apoptosis of human pituitary adenoma HP75 cells via mitochondrial pathway.
authors.affiliation: Department of Neurosurgery, People's Hospital of Anqiu City Anqiu 262100, Shandong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, People's Hospital of Anqiu City Anqiu 262100, Shandong, China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Weifang Medical University Weifang 261031, Shandong, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, People's Hospital of Anqiu City Anqiu 262100, Shandong, China.
authors.forename: Huanzhi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D055786:Gene Knockout Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering; D014162:Transfection; D000071165:Trefoil Factor-3
chemical_list: D010455:Peptides; D034741:RNA, Small Interfering; C082495:TFF3 protein, human; D000071165:Trefoil Factor-3
keywords: HP75 cell; RNA interference; Trefoil factor 3; cell apoptosis; pituitary adenoma
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