title: Antimicrobial Properties of a Potential Probiotic Lactobacillus from Thai Newborn Feces.
abstract: BACKGROUND
  Probiotics are increasingly used to treat infectious diarrhea and antibiotic-associated diarrhea. Many probiotic bacteria are classified in general such as Lactobacillus and are able to colonize the gastrointestinal tracts of infants.
  
  
  OBJECTIVE
  This study was performed to detect antimicrobial substances and activity in 200 Lactobacillus isolates obtained from healthy Thai newborn feces.
  
  
  METHODS
  Reuterin production was detected by the spot overlay technique and colorimetric assay. Antimicrobial activity was tested by using a well diffusion, agar method.
  
  
  RESULTS
  Lactobacillus strain MSMC64-1 produced reuterin and demonstrated potent antimicrobial activity against seven pathogenic indicator strains with very strong inhibitory activities against Salmonella typhi DMST 5784 and methicillin resistant Staphylococcus aureus (MRSA) DMST 20651. There was strong inhibitory activity against methicillin resistant Staphylococcus aureus (MRSA) DMST20654, Vibrio parahaemolyticus DMST 5665 and Shigella dysenteriae DMST 15111. There was moderate to weak inhibitory activities against Vibrio cholerae DMST 2873 and Helicobacter pylori (H40). The Lactobacillus strain MSMC 64-1 showed resistance to acidic pH (pH 2, 3, 4) and tolerance to 1%, 2%, 3%, and 4% bile concentrations. Sequencing of the 16S ribosomal DNA identified the candidate's strain as Lactobacillus reuteri with 98% sequence homology.
  
  
  CONCLUSIONS
  The active isolate could potentially be used as a probiotic to prevent and treat enteric infections.
authors.forename: Juntana
authors.initials: J
authors.lastname: Chimchang
authors.forename: Talent
authors.initials: T
authors.lastname: Theparee
authors.forename: Boonyarut
authors.initials: B
authors.lastname: Ladda
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.forename: Benjamas Thanomsub
authors.initials: BT
authors.lastname: Wongsatayanon
authors.forename: Malai
authors.initials: M
authors.lastname: Taweechotipatr
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D005243:Feces / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D007778:Lactobacillus / Q000302:isolation & purification*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D019936:Probiotics / Q000302:isolation & purification / Q000494:pharmacology*; D011407:Propane / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D011407:Propane