title: Advances in genetic engineering of the avian genome: "Realising the promise".
abstract: This review provides an historic perspective of the key steps from those reported at the 1st Transgenic Animal Research Conference in 1997 through to the very latest developments in avian transgenesis. Eighteen years later, on the occasion of the 10th conference in this series, we have seen breakthrough advances in the use of viral vectors and transposons to transform the germline via the direct manipulation of the chicken embryo, through to the establishment of PGC cultures allowing in vitro modification, expansion into populations to analyse the genetic modifications and then injection of these cells into embryos to create germline chimeras. We have now reached an unprecedented time in the history of chicken transgenic research where we have the technology to introduce precise, targeted modifications into the chicken genome, ranging from; new transgenes that provide improved phenotypes such as increased resilience to economically important diseases; the targeted disruption of immunoglobulin genes and replacement with human sequences to generate transgenic chickens that express "humanised" antibodies for biopharming; and the deletion of specific nucleotides to generate targeted gene knockout chickens for functional genomics. The impact of these advances is set to be realised through applications in chickens, and other bird species as models in scientific research, for novel biotechnology and to protect and improve agricultural productivity.
authors.affiliation: Australian Animal Health Laboratory, CSIRO Health and Biosecurity, Private Bag 24, Geelong, VIC, 3220, Australia. timothy.doran@csiro.au.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Doran
authors.affiliation: Australian Animal Health Laboratory, CSIRO Health and Biosecurity, Private Bag 24, Geelong, VIC, 3220, Australia.
authors.forename: Caitlin A
authors.initials: CA
authors.lastname: Cooper
authors.affiliation: Australian Animal Health Laboratory, CSIRO Health and Biosecurity, Private Bag 24, Geelong, VIC, 3220, Australia.
authors.forename: Kristie A
authors.initials: KA
authors.lastname: Jenkins
authors.affiliation: Australian Animal Health Laboratory, CSIRO Health and Biosecurity, Private Bag 24, Geelong, VIC, 3220, Australia.
authors.forename: Mark L V
authors.initials: ML
authors.lastname: Tizard
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001709:Biotechnology / Q000639:trends*; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D005818:Genetic Engineering / Q000639:trends*; D016678:Genome; D005854:Germ Cells; D006801:Humans
keywords: Avian; Genetic engineering; Genome engineering; Poultry; Transgenic chicken
references.citation: Trends Biotechnol. 2006 Mar;24(3):99-101
references.pmid: 16445998
references.citation: PLoS One. 2012;7(12):e50817
references.pmid: 23251391
references.citation: Dev Biol. 2007 May 15;305(2):616-24
references.pmid: 17362912
references.citation: Biol Reprod. 2014 Jan 23;90(1):15
references.pmid: 24337317
references.citation: Nat Biotechnol. 2013 Mar;31(3):230-2
references.pmid: 23360966
references.citation: Drug Discov Today. 2005 Feb 1;10(3):191-6
references.pmid: 15708533
references.citation: FASEB J. 2008 Jul;22(7):2435-44
references.pmid: 18263695
references.citation: Nat Methods. 2009 Jun;6(6):415-22
references.pmid: 19478801
references.citation: Nat Biotechnol. 2014 Jul;32(7):599-601
references.pmid: 25004207
references.citation: Cell Biol Int. 1997 Aug;21(8):495-9
references.pmid: 9451806
references.citation: Gene. 1998 Dec 28;225(1-2):17-22
references.pmid: 9931412
references.citation: Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):E1466-72
references.pmid: 22586100
references.citation: Nature. 1994 Apr 28;368(6474):856-9
references.pmid: 8159246
references.citation: Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12716-21
references.pmid: 25139993
references.citation: Mol Reprod Dev. 1994 Jul;38(3):268-74
references.pmid: 7917278
references.citation: Nature. 2012 Nov 1;491(7422):114-8
references.pmid: 23000899
references.citation: Biol Reprod. 2010 Jun;82(6):1057-64
references.pmid: 20147736
references.citation: Biol Reprod. 2003 May;68(5):1657-62
references.pmid: 12606438
references.citation: Nat Biotechnol. 2009 Feb;27(2):173-81
references.pmid: 19151699
references.citation: EMBO Rep. 2003 Nov;4(11):1054-60
references.pmid: 14566324
references.citation: Transgenic Res. 2013 Dec;22(6):1257-64
references.pmid: 23807321
references.citation: Development. 1996 Aug;122(8):2339-48
references.pmid: 8756279
references.citation: Bioessays. 2009 Apr;31(4):459-65
references.pmid: 19274658
references.citation: Comp Immunol Microbiol Infect Dis. 2009 Mar;32(2):61-80
references.pmid: 18249442
references.citation: PLoS One. 2014 Jun 25;9(6):e100905
references.pmid: 24964090
references.citation: J Exp Zool. 2001 Dec 1;290(7):691-9
references.pmid: 11748617
references.citation: Methods Cell Biol. 2011;104:51-8
references.pmid: 21924156
references.citation: Cell. 2013 May 9;153(4):910-8
references.pmid: 23643243
references.citation: EMBO Rep. 2004 Jul;5(7):728-33
references.pmid: 15192698
references.citation: Science. 2002 Feb 1;295(5556):868-72
references.pmid: 11786607
references.citation: Science. 1996 Apr 12;272(5259):263-7
references.pmid: 8602510
references.citation: Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20170-5
references.pmid: 24282302
references.citation: Nat Biotechnol. 2002 Sep;20(9):889-94
references.pmid: 12172556
references.citation: Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2140-5
references.pmid: 11854510
references.citation: Biochem Biophys Res Commun. 2001 Aug 24;286(3):456-63
references.pmid: 11511080
references.citation: Nat Biotechnol. 2013 Mar;31(3):227-9
references.pmid: 23360964
references.citation: Theor Appl Genet. 1989 Apr;77(4):457-61
references.pmid: 24232709
references.citation: J Immunol. 2013 Feb 15;190(4):1481-90
references.pmid: 23303672
references.citation: PLoS One. 2010 Nov 29;5(11):e15518
references.pmid: 21124737
references.citation: Poult Sci. 2003 Jun;82(6):927-30
references.pmid: 12817447
references.citation: Nature. 1981 Nov 5;294(5836):92-4
references.pmid: 6945481
references.citation: Dev Dyn. 2003 Mar;226(3):439-45
references.pmid: 12619130
references.citation: Virology. 1989 Sep;172(1):156-69
references.pmid: 2549707
references.citation: Dev Dyn. 2004 Mar;229(3):414-21
references.pmid: 14991696
references.citation: J Reprod Fertil. 1994 Nov;102(2):321-5
references.pmid: 7861384
references.citation: Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1156-60
references.pmid: 8577732
references.citation: Science. 2013 Feb 15;339(6121):819-23
references.pmid: 23287718
references.citation: Science. 1981 Dec 11;214(4526):1244-6
references.pmid: 6272397
references.citation: Genome Res. 2014 Jan;24(1):125-31
references.pmid: 24253447
references.citation: Trends Mol Med. 2005 Jun;11(6):293-8
references.pmid: 15949771
references.citation: Science. 2013 Feb 15;339(6121):823-6
references.pmid: 23287722
references.citation: J Virol. 1989 Jun;63(6):2680-9
references.pmid: 2542581
references.citation: Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):12013-7
references.pmid: 21730124
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1771-6
references.pmid: 17259305
references.citation: Nature. 2006 Jun 8;441(7094):766-9
references.pmid: 16760981
references.citation: Nat Rev Genet. 2003 Oct;4(10):825-33
references.pmid: 14526378
references.citation: Theriogenology. 2002 Nov;58(8):1531-9
references.pmid: 12374123
references.citation: Neural Dev. 2012 Jun 27;7:22
references.pmid: 22738172
references.citation: Anim Genet. 2008 Apr;39(2):180-6
references.pmid: 18318790
references.citation: Poult Sci. 1999 Feb;78(2):230-4
references.pmid: 10051036
references.citation: Mol Reprod Dev. 2008 Jul;75(7):1163-75
references.pmid: 18213680
references.citation: Nat Biotechnol. 2011 Jan;29(1):64-7
references.pmid: 21151125
references.citation: Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9337-41
references.pmid: 22645326
references.citation: PLoS One. 2015 Apr 13;10(4):e0124768
references.pmid: 25867079
references.citation: Science. 2011 Jan 14;331(6014):223-6
references.pmid: 21233391