title: Designed synthesis of Graphene @titania @mesoporous silica hybrid material as size-exclusive metal oxide affinity chromatography platform for selective enrichment of endogenous phosphopeptides.
abstract: In this work, a novel size-exclusive metal oxide affinity chromatography (SE-MOAC) platform was built for phosphoproteome research. The operation for preparing graphene @titania @mesoporous silica nanohybrids (denoted as G@TiO2@mSiO2) was facile and easy to conduct by grafting titania nanoparticles on polydopamine (PD)-covered graphene, following a layer of mesoporous silica was coated on the outermost layer. The G@TiO2@mSiO2 nanohybrids exhibited high sensitivity with a low detection limit of 5 amol/μL (a total amount of 1 fmol) and high selectivity for phosphopeptides at a mass ratio of phosphopeptides to non-phosphopeptides (1:1000). The size-exclusive capability of the nanohybrids were also demonstrated by enriching the phosphopeptides from the mixture of Bovine Serum Albumin (BSA), α-casein, and β-casein digests with a high mass ratio (β-casein digests: α-casein: BSA, 1:500:500), which was attributed to the large surface area and ordered mesoporous channels. In addition, the G@TiO2@mSiO2 nanohybrids were employed to capture the endogenous phosphopeptides from human serum successfully.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Jizong
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Nianrong
authors.initials: N
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Zhang
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D002846:Chromatography, Affinity / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010748:Phosphopeptides / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D002364:Caseins; D007211:Indoles; D010748:Phosphopeptides; D011108:Polymers; C568283:polydopamine; C009495:titanium dioxide; D012822:Silicon Dioxide; D006108:Graphite; D014025:Titanium
keywords: Endogenous phosphopeptides; MALDI-TOF MS; Mesoporous silica; Size-exclusive; Titania