title: Focal effector accumulation in a biotrophic interface at the primary invasion sites of Colletotrichum orbiculare in multiple susceptible plants.
abstract: We identified virulence-related effectors of a hemibiotrophic fungal pathogen Colletotrichum orbiculare, and found that a novel interface was generated by a biotrophic interaction between C. orbiculare and the host cucumber, in which the effectors secreted from the pathogen accumulated preferentially. The interface was located around the biotrophic primary hyphal neck. Here, we showed that C. orbiculare also developed this interface in a biotrophic interaction with melon, which belongs to Cucurbitaceae. Furthermore, C. orbiculare developed interface in the interaction with a susceptible plant, Nicotiana benthamiana, which is distantly related to Cucurbitaceae, suggesting that the spatial regulation strategy for effectors in C. orbiculare is not specific to cucumber; rather, it is conserved among the various plants that are susceptible to this pathogen.
authors.affiliation: a Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Irieda
authors.affiliation: a Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Suthitar
authors.initials: S
authors.lastname: Ogawa
authors.affiliation: a Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Takano
mesh_terms: D020231:Colletotrichum / Q000472:pathogenicity / Q000502:physiology*; D018553:Cucumis sativus / Q000382:microbiology; D019662:Cucurbitaceae / Q000382:microbiology*; D004198:Disease Susceptibility; D054884:Host-Pathogen Interactions; D008954:Models, Biological; D010935:Plant Diseases / Q000382:microbiology*; D014026:Nicotiana / Q000382:microbiology; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D037521:Virulence Factors
keywords: Biotrophic interface; Colletotrichum orbiculare; Nicotiana benthamiana; effector; hyphal neck; melon; phytopathogenic fungus; secretion
references.citation: Curr Opin Plant Biol. 2008 Aug;11(4):404-11
references.pmid: 18499508
references.citation: Curr Opin Plant Biol. 2007 Aug;10(4):358-65
references.pmid: 17611143
references.citation: Curr Opin Plant Biol. 2012 Aug;15(4):477-82
references.pmid: 22658704
references.citation: Mol Plant Microbe Interact. 2006 Mar;19(3):270-9
references.pmid: 16570657
references.citation: PLoS Pathog. 2012;8(5):e1002711
references.pmid: 22589729
references.citation: Mol Microbiol. 2006 Apr;60(1):81-92
references.pmid: 16556222
references.citation: Plant Cell. 2006 Jan;18(1):243-56
references.pmid: 16326930
references.citation: Mol Plant Microbe Interact. 2012 May;25(5):625-36
references.pmid: 22352720
references.citation: Plant Cell. 2010 Apr;22(4):1388-403
references.pmid: 20435900
references.citation: Plant Cell. 2009 Apr;21(4):1273-90
references.pmid: 19357089
references.citation: Nature. 2012 Apr 11;484(7393):186-94
references.pmid: 22498624
references.citation: Cell Microbiol. 2011 Dec;13(12):1849-57
references.pmid: 21848815
references.citation: PLoS Pathog. 2012;8(4):e1002643
references.pmid: 22496661
references.citation: New Phytol. 2013 Mar;197(4):1236-49
references.pmid: 23252678
references.citation: Plant Cell. 2014 May 21;26(5):2265-2281
references.pmid: 24850852
references.citation: Cell Microbiol. 2011 Feb;13(2):210-26
references.pmid: 20880355
references.citation: Biotechniques. 1997 Oct;23(4):686-90
references.pmid: 9343693
references.citation: Cell Host Microbe. 2012 Oct 18;12(4):484-95
references.pmid: 23084917
references.citation: Fungal Genet Biol. 1999 Jul-Aug;27(2-3):186-98
references.pmid: 10441444