title: High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy.
abstract: We describe the construction of a prismless widefield surface plasmon microscope; this has been applied to imaging of the interactions of protein and antibodies in aqueous media. The illumination angle of spatially incoherent diffuse laser illumination was controlled with an amplitude spatial light modulator placed in a conjugate back focal plane to allow dynamic control of the illumination angle. Quantitative surface plasmon microscopy images with high spatial resolution were acquired by post-processing a series of images obtained as a function of illumination angle. Experimental results are presented showing spatially and temporally resolved binding of a protein to a ligand. We also show theoretical results calculated by vector diffraction theory that accurately predict the response of the microscope on a spatially varying sample thus allowing proper quantification and interpretation of the experimental results.
authors.affiliation: Intellectual Property Office Ministry of Economic Affairs, Da-an, Taipei, Taiwan.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Tan
authors.affiliation: Department of Electronic and Information Engineering, the Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.
authors.forename: Suejit
authors.initials: S
authors.lastname: Pechprasarn
authors.affiliation: Keele University, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke-on-Trent, Staffordshire, ST4 7QB UK.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Natural Power, The Old Barns, Fair Oaks Farm, Hollybush, HR8 1EU, UK.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Pitter
authors.affiliation: Department of Electronic and Information Engineering, the Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Somekh
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