title: Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging.
abstract: Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives.
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, F-75005, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Quérard
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, F-75005, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Le Saux
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, F-75005, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Gautier
authors.affiliation: INSERM U1065, C3M, 151 route Saint Antoine de Ginestière, BP 2 3194, F-06204, Nice Cedex 3, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Alcor
authors.affiliation: Ecole Normale Supérieure, Département de Physique and Département de Biologie, Laboratoire de Physique Statistique UMR CNRS-ENS 8550, 24 rue Lhomond, F-75005, Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Croquette
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Laboratoire de Physique Théorique de la Matière Condensée, 4 place Jussieu, case courrier 121, 75252, Paris cedex 05, France.|CNRS, UMR 7600 LPTMC, 75005, Paris, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Lemarchand
authors.affiliation: Laboratoire de Photonique et de Nanostructures, LPN-CNRS, route de Nozay, 91460, Marcoussis, France.
authors.forename: Charlie
authors.initials: C
authors.lastname: Gosse
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, F-75005, Paris, France. Ludovic.Jullien@ens.fr.|Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005, Paris, France. Ludovic.Jullien@ens.fr.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France. Ludovic.Jullien@ens.fr.
authors.forename: Ludovic
authors.identifier: 0000-0001-8561-8077
authors.initials: L
authors.lastname: Jullien
mesh_terms: D007700:Kinetics; D057054:Molecular Imaging; D011312:Pressure; D013057:Spectrum Analysis / Q000379:methods; D013696:Temperature
keywords: cell imaging; chemical kinetics; dynamic contrast; imaging protocols; reactive modules