title: A Specific Transcriptome Signature for Guard Cells from the C4 Plant Gynandropsis gynandra.
abstract: C4 photosynthesis represents an excellent example of convergent evolution that results in the optimization of both carbon and water usage by plants. In C4 plants, a carbon-concentrating mechanism divided between bundle sheath and mesophyll cells increases photosynthetic efficiency. Compared with C3 leaves, the carbon-concentrating mechanism of C4 plants allows photosynthetic operation at lower stomatal conductance, and as a consequence, transpiration is reduced. Here, we characterize transcriptomes from guard cells in C3 Tareneya hassleriana and C4 Gynandropsis gynandra belonging to the Cleomaceae. While approximately 60% of Gene Ontology terms previously associated with guard cells from the C3 model Arabidopsis (Arabidopsis thaliana) are conserved, there is much less overlap between patterns of individual gene expression. Most ion and CO2 signaling modules appear unchanged at the transcript level in guard cells from C3 and C4 species, but major variations in transcripts associated with carbon-related pathways known to influence stomatal behavior were detected. Genes associated with C4 photosynthesis were more highly expressed in guard cells of C4 compared with C3 leaves. Furthermore, we detected two major patterns of cell-specific C4 gene expression within the C4 leaf. In the first, genes previously associated with preferential expression in the bundle sheath showed continually decreasing expression from bundle sheath to mesophyll to guard cells. In the second, expression was maximal in the mesophyll compared with both guard cells and bundle sheath. These data imply that at least two gene regulatory networks act to coordinate gene expression across the bundle sheath, mesophyll, and guard cells in the C4 leaf.
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementales, 13108 Saint-Paul-lez-Durance, France (O.A., B.G.).
authors.forename: Sylvain
authors.identifier: http://orcid.org/0000-0002-7598-3609
authors.initials: S
authors.lastname: Aubry
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementales, 13108 Saint-Paul-lez-Durance, France (O.A., B.G.).
authors.forename: Olga
authors.initials: O
authors.lastname: Aresheva
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementales, 13108 Saint-Paul-lez-Durance, France (O.A., B.G.).
authors.forename: Ivan
authors.initials: I
authors.lastname: Reyna-Llorens
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementales, 13108 Saint-Paul-lez-Durance, France (O.A., B.G.).
authors.forename: Richard D
authors.identifier: http://orcid.org/0000-0001-8721-7197
authors.initials: RD
authors.lastname: Smith-Unna
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementales, 13108 Saint-Paul-lez-Durance, France (O.A., B.G.) jmh65@cam.ac.uk bernard.genty@cea.fr.
authors.forename: Julian M
authors.identifier: http://orcid.org/0000-0003-0662-7958
authors.initials: JM
authors.lastname: Hibberd
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementales, 13108 Saint-Paul-lez-Durance, France (O.A., B.G.) jmh65@cam.ac.uk bernard.genty@cea.fr.
authors.forename: Bernard
authors.initials: B
authors.lastname: Genty
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics; D031485:Cleome / Q000166:cytology* / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D019684:Magnoliopsida / Q000166:cytology / Q000235:genetics; D058503:Mesophyll Cells / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D054046:Plant Stomata / Q000166:cytology / Q000378:metabolism; D015398:Signal Transduction; D013045:Species Specificity; D059467:Transcriptome
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