title: Overexpression of a Stress-Responsive NAC Transcription Factor Gene ONAC022 Improves Drought and Salt Tolerance in Rice.
abstract: The NAC transcription factors play critical roles in regulating stress responses in plants. However, the functions for many of the NAC family members in rice are yet to be identified. In the present study, a novel stress-responsive rice NAC gene, ONAC022, was identified. Expression of ONAC022 was induced by drought, high salinity, and abscisic acid (ABA). The ONAC022 protein was found to bind specifically to a canonical NAC recognition cis-element sequence and showed transactivation activity at its C-terminus in yeast. The ONAC022 protein was localized to nucleus when transiently expressed in Nicotiana benthamiana. Three independent transgenic rice lines with overexpression of ONAC022 were generated and used to explore the function of ONAC022 in drought and salt stress tolerance. Under drought stress condition in greenhouse, soil-grown ONAC022-overexpressing (N22oe) transgenic rice plants showed an increased drought tolerance, leading to higher survival ratios and better growth than wild-type (WT) plants. When grown hydroponically in Hogland solution supplemented with 150 mM NaCl, the N22oe plants displayed an enhanced salt tolerance and accumulated less Na(+) in roots and shoots as compared to WT plants. Under drought stress condition, the N22oe plants exhibited decreased rates of water loss and transpiration, reduced percentage of open stomata and increased contents of proline and soluble sugars. However, the N22oe lines showed increased sensitivity to exogenous ABA at seed germination and seedling growth stages but contained higher level of endogenous ABA. Expression of some ABA biosynthetic genes (OsNCEDs and OsPSY), signaling and regulatory genes (OsPP2C02, OsPP2C49, OsPP2C68, OsbZIP23, OsAP37, OsDREB2a, and OsMYB2), and late stress-responsive genes (OsRAB21, OsLEA3, and OsP5CS1) was upregulated in N22oe plants. Our data demonstrate that ONAC022 functions as a stress-responsive NAC with transcriptional activator activity and plays a positive role in drought and salt stress tolerance through modulating an ABA-mediated pathway.
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Hong
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Song
keywords: NAC transcription factor; ONA022; abscisic acid (ABA); drought tolerance; rice (Oryza sativa L.); salt tolerance
references.citation: Mol Genet Genomics. 2008 Dec;280(6):547-63
references.pmid: 18813954
references.citation: Mol Genet Genomics. 2010 Sep;284(3):173-83
references.pmid: 20632034
references.citation: Biochim Biophys Acta. 2012 Feb;1819(2):120-8
references.pmid: 21964328
references.citation: Plant Physiol. 2010 May;153(1):185-97
references.pmid: 20335401
references.citation: Trends Plant Sci. 2012 Jun;17(6):369-81
references.pmid: 22445067
references.citation: Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):10013-8
references.pmid: 24951508
references.citation: Cell Res. 2008 Apr;18(4):508-21
references.pmid: 18071364
references.citation: Anal Sci. 2012;28(11):1081-7
references.pmid: 23149609
references.citation: Trends Plant Sci. 2007 Sep;12(9):419-26
references.pmid: 17698401
references.citation: Mol Plant Microbe Interact. 2007 Jul;20(7):740-50
references.pmid: 17601162
references.citation: Mol Plant Microbe Interact. 2014 Oct;27(10 ):1027-34
references.pmid: 25014590
references.citation: Annu Rev Plant Biol. 2014;65:715-41
references.pmid: 24313844
references.citation: PLoS One. 2014 Mar 25;9(3):e92913
references.pmid: 24667379
references.citation: J Exp Bot. 2000 Sep;51(350):1563-74
references.pmid: 11006307
references.citation: Biochim Biophys Acta. 2012 Feb;1819(2):86-96
references.pmid: 21867785
references.citation: Annu Rev Plant Biol. 2002;53:247-73
references.pmid: 12221975
references.citation: Plant Mol Biol. 1994 Aug;25(5):791-8
references.pmid: 8075396
references.citation: Front Plant Sci. 2015 Sep 09;6:676
references.pmid: 26442000
references.citation: Biochem Biophys Res Commun. 2009 Feb 20;379(4):985-9
references.pmid: 19135985
references.citation: Front Microbiol. 2013 Sep 03;4:248
references.pmid: 24058359
references.citation: J Plant Physiol. 2010 May 1;167(7):571-7
references.pmid: 19962211
references.citation: Front Plant Sci. 2014 Apr 22;5:151
references.pmid: 24795738
references.citation: Plant Sci. 2014 Jun;223:1-7
references.pmid: 24767109
references.citation: BMC Genomics. 2010 Jul 16;11:435
references.pmid: 20637108
references.citation: Plant Physiol. 2010 Nov;154(3):1304-18
references.pmid: 20852032
references.citation: Plant Physiol. 2008 Dec;148(4):1938-52
references.pmid: 18931143
references.citation: Theor Appl Genet. 2007 Jun;115(1):35-46
references.pmid: 17426956
references.citation: DNA Res. 2003 Dec 31;10(6):239-47
references.pmid: 15029955
references.citation: Int J Mol Sci. 2015 Feb 17;16(2):4306-26
references.pmid: 25690040
references.citation: Annu Rev Plant Biol. 2006;57:781-803
references.pmid: 16669782
references.citation: Front Plant Sci. 2014 May 16;5:170
references.pmid: 24904597
references.citation: Planta. 2011 Aug;234(2):331-45
references.pmid: 21448719
references.citation: Trends Plant Sci. 2005 Feb;10(2):88-94
references.pmid: 15708346
references.citation: J Exp Bot. 2007;58(2):221-7
references.pmid: 17075077
references.citation: New Phytol. 2013 Aug;199(3):639-49
references.pmid: 24010138
references.citation: Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15898-903
references.pmid: 12456878
references.citation: Plant Biotechnol J. 2012 Sep;10(7):792-805
references.pmid: 22551450
references.citation: Trends Plant Sci. 2005 Feb;10(2):79-87
references.pmid: 15708345
references.citation: Plant Cell. 2004 Sep;16(9):2481-98
references.pmid: 15319476
references.citation: Genes Dev. 2000 Dec 1;14 (23 ):3024-36
references.pmid: 11114891
references.citation: Plant J. 2009 Feb;57(4):615-25
references.pmid: 18980655
references.citation: Plant Mol Biol. 2013 Jan;81(1-2):41-56
references.pmid: 23103994
references.citation: Plant Cell. 2002;14 Suppl:S165-83
references.pmid: 12045276
references.citation: Biochem J. 1958 Apr;68(4):669-72
references.pmid: 13522678
references.citation: Plant Physiol. 2009 Jul;150(3):1368-79
references.pmid: 19429605
references.citation: Plant Mol Biol. 2002 Sep;50(2):237-48
references.pmid: 12175016
references.citation: PLoS One. 2014 Jan 28;9(1):e86895
references.pmid: 24489802
references.citation: Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15354-61
references.pmid: 10611388
references.citation: BMC Plant Biol. 2006 Oct 12;6:25
references.pmid: 17038189
references.citation: BMC Plant Biol. 2013 Sep 12;13:132
references.pmid: 24028154
references.citation: Gene. 2010 Oct 1;465(1-2):30-44
references.pmid: 20600702
references.citation: EMBO J. 2009 Apr 8;28(7):926-36
references.pmid: 19229294
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):12987-92
references.pmid: 16924117
references.citation: Front Plant Sci. 2015 Oct 29;6:902
references.pmid: 26579152
references.citation: PLoS One. 2012;7(9):e45117
references.pmid: 23024799
references.citation: Plant Biotechnol J. 2012 Jan;10(1):2-11
references.pmid: 21696534
references.citation: Plant Cell Physiol. 2014 Mar;55(3):604-19
references.pmid: 24399239
references.citation: Plant J. 2001 Aug;27(4):325-33
references.pmid: 11532178
references.citation: PLoS One. 2015 Feb 03;10(2):e0116646
references.pmid: 25647508
references.citation: Plant Physiol. 1997 Jun;114(2):591-6
references.pmid: 9193091
references.citation: Plant J. 2003 Feb;33(4):751-63
references.pmid: 12609047
references.citation: Plant J. 2007 Aug;51(4):617-30
references.pmid: 17587305
references.citation: J Exp Bot. 2012 Apr;63(7):2541-56
references.pmid: 22301384
references.citation: DNA Res. 2011 Aug;18(4):263-76
references.pmid: 21685489
references.citation: Plant Biotechnol J. 2013 Jan;11(1):101-14
references.pmid: 23094910
references.citation: J Exp Bot. 2013 Jan;64(2):569-83
references.pmid: 23202132
references.citation: Plant Mol Biol. 2008 May;67(1-2):169-81
references.pmid: 18273684