title: Characterization of basic amino acids-conjugated PAMAM dendrimers as gene carriers for human adipose-derived mesenchymal stem cells.
abstract: Since mesenchymal stem cells (MSCs) can self-renew and differentiate into multiple cell types, the delivery of genes to this type of cell can be an important tool in the emerging field of tissue regeneration and engineering. However, development of more efficient and safe nonviral vectors for gene delivery to stem cells in particular still remains a great challenge. In this study, we describe a group of nonviral gene delivery vectors, conjugated PAMAM derivatives (PAMAM-H-R, PAMAM-H-K, and PAMAM-H-O), displaying affinity toward human adipose-derived mesenchymal stem cells (AD-MSCs). Transfection efficiency using pDNA encoding for luciferase (Luc) and enhanced green fluorescent protein (EGFP), and cytotoxicity assays were performed in human AD-MSCs. The results show that transfection efficiencies of conjugated PAMAM derivatives are improved significantly compared to native PAMAM dendrimer, and that among PAMAM derivatives, cytotoxicity of PAMAM-H-K and PAMAM-H-O were very low. Also, treatment of human AD-MSCs to polyplex formation in conjugated PAMAM derivatives, their cellular uptake and localization were analyzed by flow cytometry and confocal microscopy.
authors.affiliation: Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan 614-735, Republic of Korea.
authors.forename: Yoonhee
authors.initials: Y
authors.lastname: Bae
authors.affiliation: Cell Engineering for Origin Research Center, 46-21, Susong-dog, Jongno-gu, Seoul 110-140, Republic of Korea.
authors.forename: Sunray
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biology, Salt Lake Community College, Salt Lake City, UT 84123, USA.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Green
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Sanggye Paik Hospital, Cardiovascular and Metabolic Disease Center, Inje University, Seoul 139-707, Republic of Korea.
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Ko
authors.affiliation: Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan 614-735, Republic of Korea. Electronic address: phyhanj@inje.ac.kr.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: joonsig@cnu.ac.kr.
authors.forename: Joon Sig
authors.initials: JS
authors.lastname: Choi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D024361:Amino Acids, Basic / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D001692:Biological Transport; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D050091:Dendrimers / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D010957:Plasmids
chemical_list: D024361:Amino Acids, Basic; D050091:Dendrimers; C104700:PAMAM Starburst; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA; D008156:Luciferases
keywords: Cellular uptake; Conjugated PAMAM dendrimer; Differentiation; Human AD-MSC; Transfection