title: Diterpenes from the Trunk of Abies holophylla and Their Potential Neuroprotective and Anti-inflammatory Activities.
abstract: Eleven new abietane-type diterpenes, holophyllins D-N (1-11), and 17 known analogues (12-28), were isolated from a MeOH extract of the trunk of Abies holophylla. The chemical structures of 1-11 were determined through spectroscopic data analysis, including NMR ((1)H and (13)C NMR, DEPT, (1)H-(1)H COSY, HMQC, HMBC, and NOESY) and HRFABMS methods. All isolated compounds (1-28) were evaluated for their cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-116), for their potential neuroprotective effects through induction of nerve growth factor in C6 glioma cells, and for their effects on nitric oxide levels in lipopolysaccharide-stimulated murine microglia BV2 cells.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
authors.forename: Chung Sub
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.|College of Pharmacy, Gachon University , #191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
authors.forename: Lalita
authors.initials: L
authors.lastname: Subedi
authors.affiliation: Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.|College of Pharmacy, Gachon University , #191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Chemical Technology , Daejeon 34114, Republic of Korea.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D028202:Abies / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects; D015394:Molecular Structure; D020932:Nerve Growth Factor / Q000187:drug effects; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009569:Nitric Oxide / Q000032:analysis; D018547:Plant Stems / Q000737:chemistry; D056910:Republic of Korea
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D009569:Nitric Oxide; D020932:Nerve Growth Factor