title: An insight into insulin-like factor 3 regulate its receptor RXFP2 in mouse gubernaculum testis cells.
abstract: The etiology of testicular dysgenesis syndrome is multifactorial and involves abnormalities in the anatomical structures and endocrine factors. Several studies have shown that the abnormal development of the gubernaculum may affect testicular descent, and the insulin-like factor 3 (INSL3) appears to play an important role in development of the gubernaculum have been proved. INSL3 binds its specific receptor (Relaxin family peptide 2, RXFP2), which was highly expressed in gubernaculum, to produce a crucial effect in the first transabdominal descent stage, but its mechanism still remain unclear. In this study, in order to explore how does INSL3 regulate its receptor RXFP2, we cultured mouse gubernaculum testis cells in vitro, which was treated by INSL3, and examined the expression of RXFP2 in mouse gubernaculum testis cells. The results displayed that INSL3 changed RXFP2 expression, and we found that low dose INSL3 can increase RXFP2 expression, the mechanism of above-mentioned might be related with the hormesis of INSL3.
authors.affiliation: Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical CollegeShantou, Guangdong, China; Department of Radiology, The First Affiliated Hospital of Shantou University Medical CollegeShantou, Guangdong, China; Guangdong Key Laboratory of Medical Molecular ImagingShantou, Guangdong, China.
authors.forename: Shouxing
authors.initials: S
authors.lastname: Duan
authors.affiliation: Department of Pediatric Surgery, The Affiliated Maternal and Child Health Hospital of Shenzhen University Medical College Shenzhen, Guangdong, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Surgery, The Affiliated Maternal and Child Health Hospital of Shenzhen University Medical College Shenzhen, Guangdong, China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Shantou University Medical CollegeShantou, Guangdong, China; Guangdong Key Laboratory of Medical Molecular ImagingShantou, Guangdong, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Shantou University Medical CollegeShantou, Guangdong, China; Guangdong Key Laboratory of Medical Molecular ImagingShantou, Guangdong, China.
authors.forename: Zongbo
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Shantou University Medical CollegeShantou, Guangdong, China; Guangdong Key Laboratory of Medical Molecular ImagingShantou, Guangdong, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003456:Cryptorchidism / Q000378:metabolism; D005434:Flow Cytometry; D007328:Insulin / Q000378:metabolism*; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000254:growth & development*
chemical_list: D007328:Insulin; C084797:Leydig insulin-like protein; D011506:Proteins; C555886:RXFP2 protein, mouse; D043562:Receptors, G-Protein-Coupled
keywords: INSL3; RXFP2; gubernaculums; mouse
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