title: Dynamics of circulating microparticles in obesity after weight loss.
abstract: A definitive relationship between adiposity and MP production is yet to be demonstrated. The aim of our study was to prospectively evaluate the levels of microparticles (MP) in a group of 20 III degree obese patients before and after weight loss. Plasma levels of annexin V-MP, endothelial-derived MP, platelet-derived MP (CD61+ and P-Selectin+), leukocyte-derived MP, tissue factor-bearing (TF+) and CD36+MP were prospectively measured in 20 patients with III degree obesity (BMI ≥ 40 kg/m(2)) before (T0) and 3 (T3) and 12 (T12) months after sleeve gastrectomy (SLG). Obese patients had lost 18 % of their body weight at T3 and 41 % at T12. We find that considering all MP, except for endothelial-derived MP, which had significantly decreased at T3, all MP subtypes had significantly decreased at T12. At T12, subjects showed a higher median level of all types of MP, except endothelial-derived MP, compared to T3, but without a statistically significant difference. The percentages of reduction of all the MP were significantly correlated with the percentage of reduction of BMI. The reductions of leukocyte-derived, TF+ and CD36+MP were significantly correlated with the reduction of leptin. Moreover, the reductions of leukocyte-derived and CD36+MP were significantly correlated with hs-CRP decrease. The decrease of BMI post-SLG in morbid obesity was matched with a decrease of circulating MP of endothelial, platelet, leukocyte origin, TF+ and CD36+. A trend of slight increase in all MP subtypes, except endothelial-derived, was detected 12 months after gastrectomy, indicating a possible underlying slow low-grade inflammatory/hypercoagulability state from adipose tissue before the potential overt weight gain.
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy. elena.campello83@gmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Campello
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Eva
authors.initials: E
authors.lastname: Zabeo
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Radu
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Spiezia
authors.affiliation: Week Surgery, Bariatric Unit, University of Padua, Padua, Italy.
authors.forename: Mirto
authors.initials: M
authors.lastname: Foletto
authors.affiliation: Week Surgery, Bariatric Unit, University of Padua, Padua, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Prevedello
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gavasso
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Bulato
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Vettor
authors.affiliation: Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Simioni
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D000328:Adult; D001777:Blood Coagulation / Q000502:physiology; D015992:Body Mass Index; D055252:Cell-Derived Microparticles / Q000502:physiology*; D005260:Female; D005743:Gastrectomy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000503:physiopathology; D011446:Prospective Studies; D015431:Weight Loss / Q000502:physiology*
keywords: Hypercoagulability; Inflammation; Microparticles; Obesity; Tissue factor
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