title: Preparation of a novel pH optical sensor using orange (II) based on agarose membrane as support.
abstract: A novel and cost effective optical pH sensor was prepared using covalent immobilization of orange (II) indicator on the agarose membrane as solid support. The fabricated optical sensor was fixed into a sample holder of a spectrophotometer instrument for pH monitoring. Variables affecting sensor performance including pH of dye bonding to agarose membrane and dye concentration were optimized. The sensor responds to the pH changes in the range of 3.0-10.0 with a response time of 2.0 min and appropriate reproducibility (RSD ≤ 0.9%). No significant variation was observed on sensor response after increasing the ionic strength in the range of 0.0-0.5M of sodium chloride. Determination of pH using the proposed optical sensor is quick, simple, inexpensive, selective and sensitive in the pH range of 3.0-10.0.
authors.affiliation: Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, PO Box 68149-89468, Khorramabad, Iran. Electronic address: rouhollahheydari@yahoo.com.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Heydari
authors.affiliation: Young Researchers and Elite Club, Arak Branch, Islamic Azad University, Arak, Iran. Electronic address: smhosseini2007@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Department of Chemistry, Faculty of Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
authors.forename: Ahmadreza
authors.initials: A
authors.lastname: Amraei
authors.affiliation: Young Researchers and Elite Club, Arak Branch, Islamic Azad University, Arak, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mohammadzadeh
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004396:Coloring Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D009994:Osmolar Concentration; D012685:Sepharose / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D004396:Coloring Agents; D008567:Membranes, Artificial; D012685:Sepharose
keywords: Agarose membrane; Covalent immobilization; Orange (II); pH optical sensor