title: Characterization of Cyclooxygenase-2 and its induction pathways in response to high lipid diet-induced inflammation in Larmichthys crocea.
abstract: The present study was conducted to investigate the effects of a high-lipid diet (HLD) on cyclooxygenase (Cox)-2 expression and the signalling pathways related to low-grade inflammation in the large yellow croaker (Larmichthys crocea). An isolated 2508 bp cDNA clone of cox-2 contained an open reading frame spanning 1827 bp encoding a protein with 608 amino acid residues. The over-expression of cox-2 was consistent with the activation of c-Jun N-terminal kinases (JNKs) and p38 mitogen-activated protein kinase (MAPK) in HLD-fed fish. The activation of the activator protein-1 (AP-1) and the nuclear transcription factor kappa-B (NF-κB) signalling pathways in HLD-fed fish and the significant increase of cox-2 promoter-luciferase activity in vitro indicated that AP-1 and NF-κB could combine cox-2 promoter to promote its transcription, respectively. Together, HLD-induced inflammation up-regulates cox-2 expression via JNKs and p38 MAPK-dependent NF-κB and AP-1 pathways. The present study provides important insight into the signal transduction pathways involved in HLD-induced inflammation, which is detrimental to the health and production of fish as well as to the health of fish consumers.
authors.affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Ai
authors.affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Kangsen
authors.initials: K
authors.lastname: Mai
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004041:Dietary Fats / Q000009:adverse effects* / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005393:Fish Diseases / Q000139:chemically induced / Q000201:enzymology*; D029941:Fish Proteins / Q000096:biosynthesis*; D005399:Fishes / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology
chemical_list: D004041:Dietary Fats; D029941:Fish Proteins; D051546:Cyclooxygenase 2
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