title: Structure of OxyA tei: completing our picture of the glycopeptide antibiotic producing Cytochrome P450 cascade.
abstract: Cyclization of glycopeptide antibiotic precursors occurs in either three or four steps catalyzed by Cytochrome P450 enzymes. Three of these enzymes have been structurally characterized to date with the second enzyme along the pathway, OxyA, escaping structural analysis. We are now able to present the structure of OxyAtei involved in teicoplanin biosynthesis - the same enzyme recently shown to be the first active OxyA homolog. In spite of the hydrophobic character of the teicoplanin precursor, the polar active site of OxyAtei and its affinity for certain azole inhibitors hint at its preference for substrates with polar decorations.
authors.affiliation: Max Planck Institute for Medical Research, Heidelberg, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Haslinger
authors.affiliation: Max Planck Institute for Medical Research, Heidelberg, Germany.
authors.forename: Max J
authors.identifier: http://orcid.org/0000-0002-9739-6157
authors.initials: MJ
authors.lastname: Cryle
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D003500:Cyclization; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D008848:Micromonosporaceae / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017334:Teicoplanin / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017334:Teicoplanin; D003577:Cytochrome P-450 Enzyme System
keywords: Cytochrome P450; glycopeptide antibiotics; nonribosomal peptide synthesis; phenolic coupling; teicoplanin