title: Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway.
abstract: BACKGROUND
  Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays key role in the progression of some human cancers. However, the role of NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We therefore investigated the expression and function of NEAT1 in LSCC.
  
  
  METHODS
  NEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was analyzed.
  
  
  RESULTS
  NEAT1 level was significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with neck nodal metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was mediated by miR-107.
  
  
  CONCLUSIONS
  NEAT1 plays an oncogenic role in the tumorigenesis of LSCC and may serve as a potential target for therapeutic intervention.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. 350239591@qq.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. tianshiyi.hot@163.com.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. zhou15153887577@163.com.
authors.forename: Han
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. 826108898@qq.com.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. drhgq@hotmail.com.
authors.forename: Guoqing
authors.initials: G
authors.lastname: He
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. haoyang1028@yeah.net.
authors.forename: Haoyang
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. xuanlijia1992@126.com.
authors.forename: Lijia
authors.initials: L
authors.lastname: Xuan
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. 546181026@qq.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. 76202920@qq.com.
authors.forename: Linli
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. hydeyebh@sina.com.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, China. liuming002@outlook.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Fifth Affiliated Hospital, Harbin Medical University, No. 213, Jianshe Road, Daqing, China. qlm8977976@163.com.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Qu
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009368:Neoplasm Transplantation; D062085:RNA, Long Noncoding / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C549045:MIRN107 microRNA, human; D035683:MicroRNAs; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6
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