title: Terasakiella brassicae sp. nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella.
abstract: A Gram-stain-negative, motile, polyhydroxybutyrate-accumulating, aerobic, S-shaped bacterium, designated B3T, was isolated from the wastewater of a pickle-processing factory. 16S rRNA gene sequence similarity analysis showed that it was most closely related to the type strain, Terasakiella pusilla (96.6% similarity). Strain B3T was able to grow at 4-40 °C (optimum 32-37 °C), pH 5.5-9.0 (optimum 6.5-7.5) and with 0.5-8% (w/v) NaCl present (optimum 1-2%, w/v). Chemotaxonomic analysis showed that the respiratory quinone was ubiquinone Q-10, the major fatty acids included C16:0, C18:1ω7c and C16:1ω7c and/or iso-C15:2-OH. The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, aminophospholipid and three uncharacterized phospholipids. The genomic DNA G+C content of strain B3T was 42.3 mol%. The DNA-DNA relatedness value between B3T and T. pusilla DSM 9263T was 23.9%. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics of strain B3T, it represents a novel species of the genus Terasakiella, for which the name Terasakiella brassicae sp. nov. is proposed. The type strain is B3T (=KCTC 42652T=CGMCC 1.15254T). Emended descriptions of T. pusilla and the genus Terasakiella are also presented.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Shuai-Bo
authors.initials: SB
authors.lastname: Han
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Rui-Jun
authors.initials: RJ
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Biology, Xinjiang Normal University, Urumqi 830054, PRChina.
authors.forename: Dildar
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PRChina.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019649:Food Industry; D006885:Hydroxybutyrates / Q000737:chemistry; D042083:Methylocystaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D062065:Wastewater; C025204:Ubiquinone Q2