title: miR-34a inhibits the apoptosis of MDSCs by suppressing the expression of N-myc.
abstract: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of early myeloid progenitors, which possess strong immunosuppressive functions. MDSCs are found in increased numbers in infectious and inflammatory pathological conditions. However, whether microRNAs have a role in the expansion remains unclear. Here in our study, we found that overexpression of miR-34a could induce the expansion of MDSCs in the bone marrow and spleen both in chimera and transgenic mice. And further experiments demonstrated that miR-34a inhibited the apoptosis through reduced translation of N-myc without affecting the proliferation. Luciferase assay and western blotting experiments implied that N-myc is the direct target of miR-34a in MDSCs. Overexpressed mir-34a changes the cytokine expression profile in MDSCs and skewed the MDSCs to M1 phenotype. And miR-34a-overexpressed MDSCs significantly slowed down the tumor growth. Taken together, miR-34a contributes to the expansion of MDSCs by inhibiting the apoptosis via suppressing the expression of N-myc.
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Anfei
authors.initials: A
authors.lastname: Huang
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Huo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xia
authors.affiliation: Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jin
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002678:Chimera; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000378:metabolism*; D000072737:Myeloid-Derived Suppressor Cells / Q000166:cytology* / Q000378:metabolism*; D000071447:N-Myc Proto-Oncogene Protein / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D016207:Cytokines; C551848:MIRN34a microRNA, mouse; D035683:MicroRNAs; D000071447:N-Myc Proto-Oncogene Protein
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