title: Mitochondria Related Pathway Is Essential for Polysaccharides Purified from Sparassis crispa Mediated Neuro-Protection against Glutamate-Induced Toxicity in Differentiated PC12 Cells.
abstract: The present study aims to explore the neuro-protective effects of purified Sparassis crispa polysaccharides against L-glutamic acid (L-Glu)-induced differentiated PC12 (DPC12) cell damages and its underlying mechanisms. The Sparassis crispa water extract was purified by a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column. A fraction with a molecular weight of 75 kDa and a diameter of 88.9 nm, entitled SCWEA, was obtained. SCWEA was identified with a triple helix with (1→3)-linked Rha in the backbone, and (1→2) linkages and (1→6) linkages in the side bone. Our results indicated that the pre-treatment of DPC12 cells with SCWEA prior to L-Glu exposure effectively reversed the reduction on cell viability (by 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay) and reduced L-Glu-induced apoptosis (by Hoechst staining). SCWEA decreased the accumulation of intracellular reactive oxygen species, blocked Ca(2+) influx and prevented depolarization of the mitochondrial membrane potential in DPC12 cells. Furthermore, SCWEA normalized expression of anti-apoptotic proteins in L-Glu-explored DPC12 cells. These results suggested that SCWEA protects against L-Glu-induced neuronal apoptosis in DPC12 cells and may be a promising candidate for treatment against neurodegenerative disease.
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. summerhu1990@outlook.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Hu
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. xiaowangdi@outlook.com.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. archer707@163.com.
authors.forename: Junrong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. jludumy@126.com.
authors.forename: Mengyan
authors.initials: M
authors.lastname: Du
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. chengyk@jlu.edu.cn.
authors.forename: Yingkun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. liuyaorui@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. zhangning1313@mails.jlu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China. jluwangdi@outlook.com.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Science, Jilin University, Changchun 130021, China. wuyi@jlu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D018698:Glutamic Acid / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018696:Neuroprotective Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; D011134:Polysaccharides; D018698:Glutamic Acid; D002118:Calcium
keywords: Sparassis crispa; glutamate; mitochondria; neuro-protection; purified polysaccharides
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