title: Molecular Study of the G1 Haplotypes of Echinococcus granulosus from Iran Based on Cytochrome C Oxidase (Subunit 1) Sequence.
abstract: OBJECTIVE
  In this study, we attempted to identify new Echinococcus granulosus isolates in the North West provinces of Iran based on the mitochondrial cytochrome c oxidase subunit 1 (CO1) sequence.
  
  
  METHODS
  Twenty-nine hydatid cysts from sheep and goats were collected. Genomic DNAs were extracted, and a partial sequence of the CO1 gene was amplified. Polymerase chain reaction products were cloned and sequenced with M13 primers in both directions.
  
  
  RESULTS
  All Iranian isolates were located in G1 and G3 genotypes. For the first time, a new G1 haplotype in two Iranian isolates were identified.
  
  
  CONCLUSIONS
  It seems that this new haplotype was transmitted from Jordan to Iran or vice versa.
authors.affiliation: Department of Central Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran. m.esmaelizad@rvsri.ac.ir.
authors.forename: Majid
authors.initials: M
authors.lastname: Esmaelizad
authors.forename: H
authors.initials: H
authors.lastname: Zeinedin
authors.forename: Naser
authors.initials: N
authors.lastname: Razmaraii
authors.forename: Ali
authors.initials: A
authors.lastname: Mirjalili
mesh_terms: D000818:Animals; D001483:Base Sequence; D003575:Cytochromes c1 / Q000737:chemistry / Q000235:genetics*; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D004444:Echinococcosis, Hepatic / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics*; D005838:Genotype; D060005:Genotyping Techniques; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D006239:Haplotypes; D007492:Iran; D008928:Mitochondria / Q000201:enzymology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*
chemical_list: D018509:DNA, Helminth; D003575:Cytochromes c1; D003576:Electron Transport Complex IV