title: Characterization of Mycobacterium Abscessus Subtypes in Shanghai of China: Drug Sensitivity and Bacterial Epidemicity as well as Clinical Manifestations.
abstract: The aim of the study was to investigate the epidemic characteristics of Mycobacterium abscessus in Shanghai.Fifty-five strains from 55 M. abscessus pulmonary disease patients were isolated. Drug sensitivity was measured by a broth microdilution method. Subtypes of M. abscessus were identified by DNA sequencing. Multilocus sequence typing (MLST), mining spanning tree (MST), and pulsed-field gel electrophoresis (PFGE) were used to analyze sequence types (ST) and clonal complexes (CC). Clinical manifestations were assessed by CT imaging.We identified 42 A isolates, 11 M, and 2 B-subtypes. A and M were highly sensitive to tigecycline and amikacin (97.6-100%). The A-type easily developed drug resistance against clarithromycin. Both types were highly resistance to sulfonamides, moxifloxacin, doxycycline, imipenem, and tobramycin. MLST analysis identified 41 STs including 32 new STs. The MST algorithm distributed 55 isolates into 12 separate CC. The PFGE analysis exhibited 53 distinct restriction patterns and the M-type was closely clustered according to their ST and CC numbers. CT imaging showed that tree-in-bud and patch shadow were commonly observed in M-type, whereas pulmonary cavities were often found in A-type infection patients (P < 0.001).ST1 in A and ST23 in M-type were the main epidemic strains in Shanghai. The M-type appeared to be prone to epidemic nosocomial transmission.
authors.affiliation: From the Department of Clinical Laboratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine (LL); Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine (BL, HC, ZZ, JZ, TG, LX, LZ); Department of Clinical Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine (DH); Department of Radiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine (XS); and Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China (HX).
authors.forename: Liulin
authors.initials: L
authors.lastname: Luo
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Haiqing
authors.initials: H
authors.lastname: Chu
authors.forename: Dongdong
authors.initials: D
authors.lastname: Huang
authors.forename: Zhemin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Gui
authors.forename: Liyun
authors.initials: L
authors.lastname: Xu
authors.forename: Lan
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiwen
authors.initials: X
authors.lastname: Sun
authors.forename: Heping
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D058893:DNA Barcoding, Taxonomic; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D009165:Mycobacterium Infections, Nontuberculous / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D012189:Retrospective Studies; D014057:Tomography, X-Ray Computed
chemical_list: D000900:Anti-Bacterial Agents
references.citation: Pediatr Surg Int. 1998 Jul;13(5-6):406-10
references.pmid: 9639628
references.citation: J Clin Microbiol. 2003 Dec;41(12):5699-708
references.pmid: 14662964
references.citation: Annu Rev Microbiol. 1998;52:453-90
references.pmid: 9891805
references.citation: Infect Genet Evol. 2013 Dec;20:292-7
references.pmid: 24055961
references.citation: Int J Syst Evol Microbiol. 2011 Sep;61(Pt 9):2311-2313
references.pmid: 21037035
references.citation: Sci Rep. 2014 Feb 11;4:4061
references.pmid: 24515248
references.citation: Clin Microbiol Rev. 2002 Oct;15(4):716-46
references.pmid: 12364376
references.citation: J Clin Microbiol. 1993 Feb;31(2):175-8
references.pmid: 8381805
references.citation: Clin Infect Dis. 1997 Jun;24(6):1147-53
references.pmid: 9195073
references.citation: Curr Opin Infect Dis. 2014 Dec;27(6):506-10
references.pmid: 25268925
references.citation: J Clin Microbiol. 2012 Nov;50(11):3556-61
references.pmid: 22915613
references.citation: Am J Respir Crit Care Med. 2011 Feb 1;183(3):405-10
references.pmid: 20833823
references.citation: Clin Microbiol Infect. 2009 Oct;15(10):906-10
references.pmid: 19845702
references.citation: J Cyst Fibros. 2016 Mar;15(2):179-85
references.pmid: 26072272
references.citation: Lancet. 2013 May 4;381(9877):1551-60
references.pmid: 23541540
references.citation: Am J Respir Crit Care Med. 1997 Aug;156(2 Pt 2):S1-25
references.pmid: 9279284
references.citation: J Microbiol Methods. 2014 Sep;104:19-25
references.pmid: 24918987
references.citation: Am J Respir Crit Care Med. 2007 Feb 15;175(4):367-416
references.pmid: 17277290
references.citation: Clin Infect Dis. 2003 Apr 15;36(8):954-62
references.pmid: 12684906
references.citation: Respir Med. 2014 Nov;108(11):1706-12
references.pmid: 25245792