title: Fast and Reliable Quantitative Peptidomics with labelpepmatch.
abstract: The use of stable isotope tags in quantitative peptidomics offers many advantages, but the laborious identification of matching sets of labeled peptide peaks is still a major bottleneck. Here we present labelpepmatch, an R-package for fast and straightforward analysis of LC-MS spectra of labeled peptides. This open-source tool offers fast and accurate identification of peak pairs alongside an appropriate framework for statistical inference on quantitative peptidomics data, based on techniques from other -omics disciplines. A relevant case study on the desert locust Schistocerca gregaria proves our pipeline to be a reliable tool for quick but thorough explorative analyses.
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Rik
authors.initials: R
authors.lastname: Verdonck
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: De Haes
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Dries
authors.initials: D
authors.lastname: Cardoen
authors.affiliation: Research Group of Bio-informatics and Computational Genomics, Ghent University , Ghent, Belgium.
authors.forename: Gerben
authors.initials: G
authors.lastname: Menschaert
authors.affiliation: Chemistry Department, University of Konstanz , Konstanz, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Huhn
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Landuyt
authors.affiliation: CFP/CeProMa, Antwerp University , Antwerp, Belgium.|Applied Bio & Molecular Systems, Vito , Mol, Belgium.
authors.forename: Geert
authors.initials: G
authors.lastname: Baggerman
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Kurt
authors.initials: K
authors.lastname: Boonen
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Wenseleers
authors.affiliation: Biology Department, KU Leuven , Leuven, Belgium.
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D006110:Grasshoppers; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013058:Mass Spectrometry; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D012984:Software
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides
keywords: PBAN; TMAB; hugin; isotope tag; label; locust; neuropeptide; peptide; peptidomics; pyrokinin