title: First report of human parvovirus 4 detection in Iran.
abstract: Parvovirus 4 (PARV4) is an emerging and intriguing virus that currently received many attentions. High prevalence of PARV4 infection in high-risk groups such as HIV infected patients highlights the potential clinical outcomes that this virus might have. Molecular techniques were used to determine both the presence and the genotype of circulating PARV4 on previously collected serum samples from 133 HIV infected patients and 120 healthy blood donors. Nested PCR was applied to assess the presence of PARV4 DNA genome in both groups. PARV4 DNA was detected in 35.3% of HIV infected patients compared to 16.6% healthy donors. To genetically characterize the PARV4 genotype in these groups, positive samples were randomly selected and subjected for sequencing and phylogenetic analysis. All PARV4 sequences were found to be genotype 1 and clustered with the reference sequences of PARV4 genotype 1. J. Med. Virol. 88:1314-1318, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Virology, School of Public Health (SPH), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Asiyabi
authors.affiliation: Department of Virology, School of Public Health (SPH), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Nejati
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Shoja
authors.affiliation: Department of Virology, School of Public Health (SPH), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Shahmahmoodi
authors.affiliation: Department of Virology, School of Public Health (SPH), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Jalilvand
authors.affiliation: Department of Virology, School of Public Health (SPH), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farahmand
authors.affiliation: Department of Bacteriology and Virology, School of Medicine Shiraz, University of Medical Sciences, Shiraz, Iran.
authors.forename: Ali-Akbar
authors.initials: AA
authors.lastname: Gorzin
authors.affiliation: Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Najafi
authors.affiliation: Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Haji Mollahoseini
authors.affiliation: Department of Virology, School of Public Health (SPH), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Sayed Mahdi
authors.initials: SM
authors.lastname: Marashi
mesh_terms: D000328:Adult; D001782:Blood Donors; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications / Q000821:virology; D064368:Healthy Volunteers; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000150:complications / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D017992:Parvovirus / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: HIV; Iran; Parvovirus 4; genotype