title: Molecular analyses of H3N2 canine influenza viruses isolated from Korea during 2013-2014.
abstract: Canine influenza A virus (CIV) causes a respiratory disease among dog populations and is prevalent in North America and Asia. Recently, Asian H3N2 CIV infection has been of particular concern, with recent reports related to reassortants with pandemic 2009 strains, direct transmission from a human H3N2, a possibility of H3N2 CIV transmission to other mammals, and even the first outbreak of H3N2 CIVs in North America in April 2015. However, despite these global concerns, our understanding of how influenza A virus transmission impacts the overall populations of H3N2 CIVs remains incomplete. Hence, we investigated the evolutionary history of the most recent two Korean CIV isolates, A/canine/Korea/BD-1/2013 and A/canine/Korea/DG1/2014, along with 57 worldwide CIVs, using comprehensive molecular analyses based on genomic genotyping. This study presents that the new Korean CIV isolates are closely related to the predominantly circulating H3N2 CIVs with genotypes K, G, E, 3B, F, 2D, F, and 1E, carrying several mutations in antigenic and host determinant sites. Also, our findings show that the genome-wide genetic variations within the H3N2 CIVs are low; however, two antigenic protein (HA and NA) analysis demonstrates genetic diversification of the H3N2 CIVs, which evolves independently between Korea and China.
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, 175 Anayngro, Anyang, Gyeonggido, 430-757, Republic of Korea.
authors.forename: EunJung
authors.initials: E
authors.lastname: Lee
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, 175 Anayngro, Anyang, Gyeonggido, 430-757, Republic of Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, 175 Anayngro, Anyang, Gyeonggido, 430-757, Republic of Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Kim
authors.affiliation: Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggido, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, 175 Anayngro, Anyang, Gyeonggido, 430-757, Republic of Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, 175 Anayngro, Anyang, Gyeonggido, 430-757, Republic of Korea. shinyk2009@korea.kr.
authors.forename: Yeun-Kyung
authors.initials: YK
authors.lastname: Shin
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D019143:Evolution, Molecular; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D058889:Molecular Typing; D009976:Orthomyxoviridae Infections / Q000662:veterinary*; D010802:Phylogeny; D012367:RNA, Viral; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: Canine influenza virus; H3N2 CIV; Korea; Molecular analysis; Phylogenetic analysis
references.citation: Bioinformatics. 2006 Nov 1;22(21):2688-90
references.pmid: 16928733
references.citation: Nat Rev Genet. 2007 Mar;8(3):196-205
references.pmid: 17262054
references.citation: Infect Genet Evol. 2013 Mar;14:444-9
references.pmid: 23261544
references.citation: Virus Genes. 2014 Feb;48(1):56-63
references.pmid: 24101341
references.citation: Virology. 2013 Jan 20;435(2):301-7
references.pmid: 23063406
references.citation: Emerg Infect Dis. 2010 Dec;16(12):2019-21
references.pmid: 21122255
references.citation: Virus Res. 2015 Jan 2;195:135-40
references.pmid: 25218482
references.citation: Nat Rev Microbiol. 2011 Aug;9(8):590-603
references.pmid: 21747392
references.citation: Emerg Infect Dis. 2008 Mar;14(3):461-4
references.pmid: 18325262
references.citation: Syst Biol. 2012 May;61(3):539-42
references.pmid: 22357727
references.citation: Mol Biol Evol. 2013 Apr;30(4):772-80
references.pmid: 23329690
references.citation: Emerg Infect Dis. 2006 Sep;12(9):1353-60
references.pmid: 17073083
references.citation: Infect Genet Evol. 2010 Dec;10(8):1286-8
references.pmid: 20732458
references.citation: Mol Biol Evol. 2012 Jun;29(6):1695-701
references.pmid: 22319168
references.citation: J Gen Virol. 2013 Feb;94(Pt 2):293-7
references.pmid: 23329681
references.citation: Virol J. 2015;12:10
references.pmid: 25645259
references.citation: Genome Announc. 2014 Oct 02;2(5):null
references.pmid: 25278543
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Bioinformatics. 2010 Jun 15;26(12):1569-71
references.pmid: 20421198
references.citation: Infect Genet Evol. 2013 Dec;20:471-5
references.pmid: 24161411
references.citation: Vet Microbiol. 2013 Aug 30;165(3-4):281-6
references.pmid: 23618838
references.citation: Vet Immunol Immunopathol. 2010 Mar 15;134(1-2):54-60
references.pmid: 19896216
references.citation: Virus Genes. 2014 Dec;49(3):393-9
references.pmid: 25056577
references.citation: Vet Microbiol. 2013 Mar 23;162(2-4):345-52
references.pmid: 23107656
references.citation: J Gen Virol. 2015 Feb;96(Pt 2):254-8
references.pmid: 25312584
references.citation: Influenza Other Respir Viruses. 2013 May;7(3):265-70
references.pmid: 22616918
references.citation: Virus Genes. 2013 Jun;46(3):558-62
references.pmid: 23463173
references.citation: Vet Microbiol. 2012 Mar 23;155(2-4):409-16
references.pmid: 22033043
references.citation: J Virol. 2015 May;89(10):5406-18
references.pmid: 25740996
references.citation: J Gen Virol. 2012 Mar;93(Pt 3):551-4
references.pmid: 22131311
references.citation: Emerg Infect Dis. 2010 Apr;16(4):699-702
references.pmid: 20350392
references.citation: PLoS One. 2014;9(1):e84638
references.pmid: 24416256
references.citation: Mol Biol Evol. 1999 Jan;16(1):37-48
references.pmid: 10331250
references.citation: J Gen Virol. 2012 Jan;93(Pt 1):119-23
references.pmid: 21976611
references.citation: Emerg Infect Dis. 2008 May;14(5):741-6
references.pmid: 18439355
references.citation: Annu Rev Anim Biosci. 2013 Jan;1:21-42
references.pmid: 25387011
references.citation: Avian Dis. 2010 Mar;54(1 Suppl):738-48
references.pmid: 20521725
references.citation: Nat Methods. 2012 Aug;9(8):772
references.pmid: 22847109
references.citation: Anim Health Res Rev. 2010 Jun;11(1):43-51
references.pmid: 20426896
references.citation: Science. 2005 Oct 21;310(5747):482-5
references.pmid: 16186182
references.citation: Bioinformatics. 2009 Jun 1;25(11):1451-2
references.pmid: 19346325
references.citation: J Biol Chem. 2010 Sep 10;285(37):28411-7
references.pmid: 20538599
references.citation: Arch Virol. 2001 Dec;146(12):2275-89
references.pmid: 11811679
references.citation: Vet Res. 2015;46:33
references.pmid: 25888728
references.citation: Influenza Other Respir Viruses. 2012 Nov;6(6):404-16
references.pmid: 22260278
references.citation: Virol J. 2011;8:395
references.pmid: 21824442
references.citation: J Gen Virol. 2011 Oct;92(Pt 10):2350-5
references.pmid: 21715595
references.citation: Virus Genes. 2014 Oct;49(2):342-7
references.pmid: 24928168
references.citation: Vet Microbiol. 2014 Jan 10;168(1):193-6
references.pmid: 24239170
references.citation: Vet Microbiol. 2015 Jan 30;175(1):17-25
references.pmid: 25480165
references.citation: Virus Genes. 2013 Jun;46(3):554-7
references.pmid: 23355070
references.citation: J Vet Sci. 2012 Sep;13(3):219-22
references.pmid: 23000577
references.citation: J Virol. 2012 Sep;86(17):9548-9
references.pmid: 22879618
references.citation: Annu Rev Microbiol. 2008;62:403-24
references.pmid: 18785841
references.citation: Emerg Infect Dis. 2009 Jan;15(1):56-8
references.pmid: 19116051
references.citation: Vet Rec. 2012 Nov 10;171(19):477
references.pmid: 22952134
references.citation: J Vet Med Sci. 2011 Jan;73(1):55-63
references.pmid: 20798477
references.citation: Arch Virol. 2007;152(10):1925-33
references.pmid: 17577611
references.citation: J Virol. 2012 Sep;86(18):10238
references.pmid: 22923798
references.citation: Nucleic Acids Res. 2007 Jul;35(Web Server issue):W275-9
references.pmid: 17537820
references.citation: Vet Microbiol. 2012 Aug 17;158(3-4):247-58
references.pmid: 22397933
references.citation: Nature. 2005 Oct 6;437(7060):889-93
references.pmid: 16208372