title: Cell Wall N-Linked Mannoprotein Biosynthesis Requires Goa1p, a Putative Regulator of Mitochondrial Complex I in Candida albicans.
abstract: The Goa1p of Candida albicans regulates mitochondrial Complex I (CI) activities in its role as a putative CI accessory protein. Transcriptional profiling of goa1∆ revealed a down regulation of genes encoding β-oligomannosyl transferases. Herein, we present data on cell wall phenotypes of goa1∆ (strain GOA31). We used transmission electron microscopy (TEM), GPC/MALLS, and NMR to compare GOA31 to a gene-reconstituted strain (GOA32) and parental cells. We note by TEM a reduction in outer wall fibrils, increased inner wall transparency, and the loss of a defined wall layer close to the plasma membrane. GPC-MALLS revealed a reduction in high and intermediate Mw mannan by 85% in GOA31. A reduction of β-mannosyl but not α-mannosyl linkages was noted in GOA31 cells. β-(1,6)-linked glucan side chains were branched about twice as often but were shorter in length for GOA31. We conclude that mitochondrial CI energy production is highly integrated with cell wall formation. Our data also suggest that not all cell wall biosynthetic processes are dependent upon Goa1p even though it provides high levels of ATP to cells. The availability of both broadly conserved and fungal-specific mutants lacking CI subunit proteins should be useful in assessing functions of fungal-specific functions subunit proteins.
authors.affiliation: Georgetown University Medical Center, Department of Microbiology & Immunology, Washington, DC, 20057, United States of America.|Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS), Jiangsu Key laboratory of Molecular Biology for Skin Disease and STIs, Nanjing, 210029, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: She
authors.affiliation: Georgetown University Medical Center, Department of Microbiology & Immunology, Washington, DC, 20057, United States of America.
authors.forename: Richard
authors.initials: R
authors.lastname: Calderone
authors.affiliation: Department of Surgery, Department of Biomedical Sciences and Center of Excellence in Inflammation, Infectious Diseases, and Immunity, East Tennessee State University, Johnson City, Tennessee, 37614, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Kruppa
authors.affiliation: Department of Surgery, Department of Biomedical Sciences and Center of Excellence in Inflammation, Infectious Diseases, and Immunity, East Tennessee State University, Johnson City, Tennessee, 37614, United States of America.
authors.forename: Douglas
authors.initials: D
authors.lastname: Lowman
authors.affiliation: Department of Surgery, Department of Biomedical Sciences and Center of Excellence in Inflammation, Infectious Diseases, and Immunity, East Tennessee State University, Johnson City, Tennessee, 37614, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Williams
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS), Jiangsu Key laboratory of Molecular Biology for Skin Disease and STIs, Nanjing, 210029, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS), Jiangsu Key laboratory of Molecular Biology for Skin Disease and STIs, Nanjing, 210029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Georgetown University Medical Center, Department of Microbiology & Immunology, Washington, DC, 20057, United States of America.
authors.forename: Kasra
authors.initials: K
authors.lastname: Khamooshi
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS), Jiangsu Key laboratory of Molecular Biology for Skin Disease and STIs, Nanjing, 210029, China.
authors.forename: Weida
authors.initials: W
authors.lastname: Liu
authors.affiliation: Georgetown University Medical Center, Department of Microbiology & Immunology, Washington, DC, 20057, United States of America.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
mesh_terms: D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D042967:Electron Transport Complex I / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*
chemical_list: D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C025363:mannoproteins; D042967:Electron Transport Complex I
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