title: Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury.
abstract: Key clinical features of carpal tunnel syndrome and other types of cumulative trauma disorders of the hand and wrist include pain and functional disabilities. Mechanistic details remain under investigation but may involve tissue inflammation and/or fibrosis. We examined the effectiveness of modeled manual therapy (MMT) as a treatment for sensorimotor behavior declines and increased fibrogenic processes occurring in forearm tissues of rats performing a high repetition high force (HRHF) reaching and grasping task for 12 weeks. Young adult, female rats were examined: food restricted control rats (FRC, n=12); rats that were trained for 6 weeks before performing the HRHF task for 12 weeks with no treatment (HRHF-CON, n=11); and HRHF task rats received modeled manual therapy (HRHF-MMT, n=5) for 5 days/week for the duration of the 12-week of task. Rats receiving the MMT expressed fewer discomfort-related behaviors, and performed progressively better in the HRHF task. Grip strength, while decreased after training, improved following MMT. Fibrotic nerve and connective tissue changes (increased collagen and TGF-β1 deposition) present in 12-week HRHF-CON rats were significantly decreased in 12-week HRHF-MMT rats. These observations support the investigation of manual therapy as a preventative for repetitive motion disorders.
authors.affiliation: Department of Biomedical Sciences, University of New England College of Osteopathic Medicine, 11 Hills Beach Rd, Biddeford, ME 04005, USA.
authors.forename: Geoffrey M
authors.initials: GM
authors.lastname: Bove
authors.affiliation: Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA, USA.
authors.forename: Michele Y
authors.initials: MY
authors.lastname: Harris
authors.affiliation: Department of Clinical Sciences and Biostatistical Consulting Center, Temple University School of Medicine, Philadelphia, PA, USA.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA, USA. Electronic address: mary.barbe@temple.edu.
authors.forename: Mary F
authors.initials: MF
authors.lastname: Barbe
mesh_terms: D000818:Animals; D003238:Connective Tissue / Q000473:pathology*; D012090:Cumulative Trauma Disorders / Q000097:blood / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis / Q000097:blood / Q000473:pathology / Q000628:therapy*; D005552:Forelimb / Q000473:pathology*; D053580:Muscle Strength / Q000502:physiology; D026201:Musculoskeletal Manipulations / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000097:blood; D016896:Treatment Outcome
chemical_list: D053773:Transforming Growth Factor beta1
keywords: Collagen; Cumulative trauma disorder; Fibrosis; Massage; Mobilization; Overuse injury; Repetitive strain injury; TGF-β1
references.citation: Clin Biomech (Bristol, Avon). 2014 Jun;29(6):671-8
references.pmid: 24861827
references.citation: Infect Immun. 2001 Mar;69(3):1847-55
references.pmid: 11179363
references.citation: Arthritis Rheum. 2007 Apr 15;57(3):495-500
references.pmid: 17394178
references.citation: Arthritis Rheum. 2007 Nov;56(11):3626-33
references.pmid: 17968889
references.citation: BMC Complement Altern Med. 2014 Oct 13;14:393
references.pmid: 25310893
references.citation: Ann Intern Med. 2002 Mar 19;136(6):471-6
references.pmid: 11900500
references.citation: J Cell Commun Signal. 2015 Mar;9(1):37-54
references.pmid: 25617052
references.citation: Clin Orthop Relat Res. 1980 Nov-Dec;(153):283-9
references.pmid: 7449228
references.citation: Cochrane Database Syst Rev. 2012 Jun 13;(6):CD009899
references.pmid: 22696387
references.citation: Folia Histochem Cytobiol. 2015;53(2):145-51
references.pmid: 26150286
references.citation: J Physiol Pharmacol. 2010 Oct;61(5):559-63
references.pmid: 21081799
references.citation: J Orthop Res. 2001 Jul;19(4):565-72
references.pmid: 11518263
references.citation: Med Sci Sports Exerc. 2013 Jun;45(6):1105-12
references.pmid: 23274593
references.citation: J Orthop Res. 2006 Mar;24(3):393-400
references.pmid: 16479573
references.citation: J Physiol. 2007 Aug 1;582(Pt 3):1303-16
references.pmid: 17540706
references.citation: PLoS One. 2013 Aug 28;8(8):e71875
references.pmid: 24015193
references.citation: J Bone Joint Surg Am. 2004 Jul;86(7):1458-66
references.pmid: 15252093
references.citation: J Sports Med Phys Fitness. 2005 Sep;45(3):370-80
references.pmid: 16230990
references.citation: J Exerc Rehabil. 2014 Apr 30;10(2):136-40
references.pmid: 24877051
references.citation: Spine (Phila Pa 1976). 2007 Feb 15;32(4):413-6
references.pmid: 17304130
references.citation: Br J Sports Med. 2008 Oct;42(10):834-8
references.pmid: 18385196
references.citation: PLoS One. 2012;7(5):e38359
references.pmid: 22675458
references.citation: Br J Sports Med. 2003 Feb;37(1):72-5
references.pmid: 12547748
references.citation: J Neurotrauma. 2003 Jul;20(7):681-95
references.pmid: 12908929
references.citation: J Neurophysiol. 2003 Sep;90(3):1949-55
references.pmid: 12724363
references.citation: Ann Occup Environ Med. 2014 Jun 24;26:14
references.pmid: 25089204
references.citation: Ergonomics. 2012;55(2):173-82
references.pmid: 21846285
references.citation: J Reconstr Microsurg. 2012 Nov;28(9):581-7
references.pmid: 22711205
references.citation: J Altern Complement Med. 2008 Apr;14(3):259-67
references.pmid: 18370581
references.citation: Ann Biomed Eng. 2014 Jan;42(1):36-49
references.pmid: 23943071
references.citation: J Orthop Res. 2010 Mar;28(3):298-307
references.pmid: 19743505
references.citation: J Bodyw Mov Ther. 2011 Jul;15(3):335-42
references.pmid: 21665110
references.citation: Oman Med J. 2011 Nov;26(6):385-91
references.pmid: 22253945
references.citation: Ann Plast Surg. 1987 Nov;19(5):430-5
references.pmid: 3688790
references.citation: Clin Sci (Lond). 2007 Mar;112(5):305-14
references.pmid: 17064252
references.citation: J Musculoskelet Neuronal Interact. 2014 Mar;14(1):78-94
references.pmid: 24583543
references.citation: BMC Musculoskelet Disord. 2013 Oct 25;14:303
references.pmid: 24156755
references.citation: J Pathol. 2004 Dec;204(5):605-12
references.pmid: 15538733
references.citation: Arch Phys Med Rehabil. 2014 Jun;95(6):1127-34
references.pmid: 24583315
references.citation: Br J Sports Med. 2013 Jan;47(2):83-8
references.pmid: 22736207
references.citation: Neuroscience. 2010 Oct 27;170(3):929-41
references.pmid: 20673790
references.citation: Exp Neurol. 2009 Sep;219(1):223-7
references.pmid: 19477176
references.citation: Am J Ind Med. 2002 Apr;41(4):221-35
references.pmid: 11920966
references.citation: Ann N Y Acad Sci. 2013 Apr;1282:1-11
references.pmid: 23496247
references.citation: Biomed Res Int. 2014;2014:878095
references.pmid: 24900996
references.citation: Cytokine. 2011 May;54(2):149-53
references.pmid: 21371906
references.citation: Int Arch Occup Environ Health. 2011 Oct;84(7):745-51
references.pmid: 21153734
references.citation: J Cell Physiol. 2008 Feb;214(2):389-95
references.pmid: 17654495
references.citation: Am Fam Physician. 2005 Sep 1;72(5):811-8
references.pmid: 16156339
references.citation: J Biomech. 2012 Oct 11;45(15):2630-6
references.pmid: 22944344
references.citation: J Mot Behav. 2011;43(6):465-76
references.pmid: 22087754
references.citation: J Neuroimmunol. 2009 Aug 18;213(1-2):26-30
references.pmid: 19564053
references.citation: Neuroscience. 2009 Jan 23;158(2):922-31
references.pmid: 19032977
references.citation: J Orthop Sports Phys Ther. 2004 May;34(5):244-53
references.pmid: 15189016
references.citation: Physiol Rep. 2015 Feb 22;3(2):
references.pmid: 25713324
references.citation: Neurosci Lett. 2009 Apr 17;454(1):33-7
references.pmid: 19429049
references.citation: J Neurophysiol. 2013 Oct;110(7):1663-71
references.pmid: 23864372
references.citation: J Appl Physiol (1985). 2007 Feb;102(2):755-61
references.pmid: 17068209
references.citation: PLoS One. 2012;7(1):e29831
references.pmid: 22238664
references.citation: J Hand Surg Am. 1982 Mar;7(2):170-5
references.pmid: 7069172
references.citation: J Cell Physiol. 2010 Oct;225(1):152-67
references.pmid: 20458732
references.citation: Sci Rep. 2015 Jul 06;5:11774
references.pmid: 26145718
references.citation: J Electromyogr Kinesiol. 2004 Feb;14(1):71-5
references.pmid: 14759752
references.citation: Scand J Gastroenterol. 2015 Mar;50(3):312-20
references.pmid: 25581784
references.citation: J Athl Train. 2005 Jul-Sep;40(3):174-80
references.pmid: 16284637
references.citation: J Can Chiropr Assoc. 2012 Sep;56(3):192-200
references.pmid: 22997469
references.citation: J Orthop Res. 2014 Jan;32(1):116-22
references.pmid: 24014274
references.citation: Med Sci Sports Exerc. 2006 May;38(5):840-6
references.pmid: 16672835
references.citation: Physiol Rev. 2004 Apr;84(2):649-98
references.pmid: 15044685
references.citation: BMC Gastroenterol. 2009 Oct 31;9:81
references.pmid: 19878580
references.citation: J Bodyw Mov Ther. 2013 Jul;17(3):332-8
references.pmid: 23768278
references.citation: Microsurgery. 2002;22(8):378-85
references.pmid: 12497576
references.citation: BMC Musculoskelet Disord. 2007 Aug 03;8:73
references.pmid: 17683540
references.citation: J Strength Cond Res. 2003 Feb;17(1):53-9
references.pmid: 12580656