title: Microbial population dynamics in response to bioaugmentation in a constructed wetland system under 10°C.
abstract: Compound microbial inocula were enriched and applied to a pilot-scale constructed wetland system to investigate their bioaugmentation effect on nitrogen removal under cold temperature (10°C). The results showed a 10% higher removal efficiency of ammonia and total nitrogen compared to a control (unbioaugmented) group. The microbial community structures before and after the bioaugmentation were analyzed through high throughput sequencing using Miseq Illumina platform. A variation of species richness and community equitability was observed in both systems. It is demonstrated that, based on the response of both the performance and microbial community, bioaugmentation using compound microbial inocula can fine tune the bacterial population and enhance the nitrogen removal efficiency of a constructed wetland system.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
authors.forename: Jixian
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
authors.forename: Shunwen
authors.initials: S
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China. Electronic address: wanglihit@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D059827:Agricultural Inoculants; D000641:Ammonia / Q000378:metabolism*; D058440:Denitrification; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D058465:Nitrification; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism*; D010865:Pilot Projects; D013696:Temperature; D018508:Water Purification / Q000379:methods; D053833:Wetlands
chemical_list: D000641:Ammonia; D009584:Nitrogen
keywords: Bacterial community; Bioaugmentation; Constructed wetland; Low temperature