title: Staphylococcus aureus and Staphylococcus epidermidis Virulence Strains as Causative Agents of Persistent Infections in Breast Implants.
abstract: Staphylococcus epidermidis and Staphylococcus aureus are currently considered two of the most important pathogens in nosocomial infections associated with catheters and other medical implants and are also the main contaminants of medical instruments. However because these species of Staphylococcus are part of the normal bacterial flora of human skin and mucosal surfaces, it is difficult to discern when a microbial isolate is the cause of infection or is detected on samples as a consequence of contamination. Rapid identification of invasive strains of Staphylococcus infections is crucial for correctly diagnosing and treating infections. The aim of the present study was to identify specific genes to distinguish between invasive and contaminating S. epidermidis and S. aureus strains isolated on medical devices; the majority of our samples were collected from breast prostheses. As a first step, we compared the adhesion ability of these samples with their efficacy in forming biofilms; second, we explored whether it is possible to determine if isolated pathogens were more virulent compared with international controls. In addition, this work may provide additional information on these pathogens, which are traditionally considered harmful bacteria in humans, and may increase our knowledge of virulence factors for these types of infections.
authors.affiliation: Microbiology and Immunology Unit, Department of Biomedical Science, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Chessa
authors.affiliation: Microbiology and Immunology Unit, Department of Biomedical Science, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Ganau
authors.affiliation: Microbiology and Immunology Unit, Department of Biomedical Science, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Luisella
authors.initials: L
authors.lastname: Spiga
authors.affiliation: Plastic Surgery Unit, Department of Surgical, Microsurgical and Medical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bulla
authors.affiliation: Microbiology and Immunology Unit, Department of Biomedical Science, School of Medicine, University of Sassari, Sassari, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Mazzarello
authors.affiliation: Plastic Surgery Unit, Department of Surgical, Microsurgical and Medical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Gian Vittorio
authors.initials: GV
authors.lastname: Campus
authors.affiliation: Microbiology and Immunology Unit, Department of Biomedical Science, School of Medicine, University of Sassari, Sassari, Italy.|Department of Infection and Immunity, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Rubino
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D019928:Breast Implantation / Q000009:adverse effects; D018427:Breast Implants / Q000382:microbiology*; D018938:Caco-2 Cells; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors
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