title: Pathogenic Free-Living Amoebae Isolated From Contact Lenses of Keratitis Patients.
abstract: BACKGROUND
  Free-living amoeba (FLA)-related keratitis is a progressive infection of the cornea with poor prognosis. The present study aimed to investigates the contact lenses of patients with keratitis for pathogenic free-living amoebae.
  
  
  METHODS
  Overall, 62 contact lenses and their paraphernalia of patients with keratitis cultured and tested for the presence of free-living amoebae using morphological criteria. Unusual plates including plates containing mix amoebae and Vermamoeba were submitted to molecular analysis.
  
  
  RESULTS
  Out of 62 plates, 11 revealed the outgrowth of free living amoeba of which 9 were Acanthamoeba, one plates contained mix amoebae including Acanthamoeba and Vermamoeba and one showed the presence of Vermamoeba. These two latter plates belonged to patients suffered from unilateral keratitis due to the misused of soft contact lenses. One of the patients had mix infection of Acanthamoeba (T4) and V. vermiformis meanwhile the other patient was infected with the V. vermiformis.
  
  
  CONCLUSIONS
  Amoebic keratitis continues to rise in Iran and worldwide. To date, various genera of free-living amoebae such as Vermamoeba could be the causative agent of keratitis. Soft contact lens wearers are the most affected patients in the country, thus awareness of high-risk people for preventing free-living amoebae related keratitis is of utmost importance.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hajialilo
authors.affiliation: Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Niyyati
authors.affiliation: Farabi Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Solaymani
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaeian
keywords: Acanthamoeba; Keratitis; Vermamoeba
references.citation: Case Rep Ophthalmol. 2011 May;2(2):291-5
references.pmid: 21941509
references.citation: Iran J Parasitol. 2012;7(2):47-52
references.pmid: 23109945
references.citation: Iran J Parasitol. 2014 Mar;9(1):14-9
references.pmid: 25642255
references.citation: Lancet. 1995 Jan 14;345(8942):136
references.pmid: 7815880
references.citation: Hum Pathol. 2013 May;44(5):918-22
references.pmid: 23332929
references.citation: Microb Pathog. 2015 Mar;80:14-20
references.pmid: 25697664
references.citation: Am J Ophthalmol. 1998 May;125(5):721-3
references.pmid: 9625566
references.citation: Cont Lens Anterior Eye. 2014 Jun;37(3):224-7
references.pmid: 24355444
references.citation: Exp Parasitol. 2014 Nov;145 Suppl:S127-30
references.pmid: 24594260
references.citation: Am J Ophthalmol. 1987 May 15;103(5):615-25
references.pmid: 3555095
references.citation: Parasitol Res. 2007 Dec;102(1):167-9
references.pmid: 17899193
references.citation: Ophthalmology. 1992 Feb;99(2):185-91
references.pmid: 1553206
references.citation: Exp Parasitol. 2010 Sep;126(1):89-90
references.pmid: 19857491
references.citation: Parasitol Res. 2011 Sep;109(3):575-80
references.pmid: 21365453
references.citation: Iran J Parasitol. 2015 Apr-Jun;10(2):157-63
references.pmid: 26246812
references.citation: Am J Ophthalmol. 2008 Jan;145(1):130-5
references.pmid: 17996208
references.citation: Ophthalmology. 1996 Mar;103(3):485-94
references.pmid: 8600427
references.citation: Parasitol Res. 2014 Jun;113(6):2395-400
references.pmid: 24802868
references.citation: J Clin Microbiol. 2001 May;39(5):1903-11
references.pmid: 11326011
references.citation: Clin Infect Dis. 1998 Nov;27(5):1337-8
references.pmid: 9827306
references.citation: Trends Parasitol. 2013 Apr;29(4):181-7
references.pmid: 23433689
references.citation: Iran J Parasitol. 2013 Jul;8(3):481-5
references.pmid: 24454444
references.citation: Parasitol Res. 2015 May;114(5):1907-12
references.pmid: 25704644
references.citation: Diagn Pathol. 2014 Jun 03;9:105
references.pmid: 24894486