title: Field-Based High-Throughput Plant Phenotyping Reveals the Temporal Patterns of Quantitative Trait Loci Associated with Stress-Responsive Traits in Cotton.
abstract: The application of high-throughput plant phenotyping (HTPP) to continuously study plant populations under relevant growing conditions creates the possibility to more efficiently dissect the genetic basis of dynamic adaptive traits. Toward this end, we employed a field-based HTPP system that deployed sets of sensors to simultaneously measure canopy temperature, reflectance, and height on a cotton (Gossypium hirsutum L.) recombinant inbred line mapping population. The evaluation trials were conducted under well-watered and water-limited conditions in a replicated field experiment at a hot, arid location in central Arizona, with trait measurements taken at different times on multiple days across 2010-2012. Canopy temperature, normalized difference vegetation index (NDVI), height, and leaf area index (LAI) displayed moderate-to-high broad-sense heritabilities, as well as varied interactions among genotypes with water regime and time of day. Distinct temporal patterns of quantitative trait loci (QTL) expression were mostly observed for canopy temperature and NDVI, and varied across plant developmental stages. In addition, the strength of correlation between HTPP canopy traits and agronomic traits, such as lint yield, displayed a time-dependent relationship. We also found that the genomic position of some QTL controlling HTPP canopy traits were shared with those of QTL identified for agronomic and physiological traits. This work demonstrates the novel use of a field-based HTPP system to study the genetic basis of stress-adaptive traits in cotton, and these results have the potential to facilitate the development of stress-resilient cotton cultivars.
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853.
authors.forename: Duke
authors.initials: D
authors.lastname: Pauli
authors.affiliation: Department of Agricultural and Biosystems Engineering, University of Arizona, Maricopa Agricultural Center, Arizona 85138.
authors.forename: Pedro
authors.initials: P
authors.lastname: Andrade-Sanchez
authors.affiliation: Arid-Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Maricopa, Arizona 85138.
authors.forename: A Elizabete
authors.initials: AE
authors.lastname: Carmo-Silva
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853.
authors.forename: Elodie
authors.initials: E
authors.lastname: Gazave
authors.affiliation: Arid-Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Maricopa, Arizona 85138.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: French
authors.affiliation: Department of Agricultural and Biosystems Engineering, University of Arizona, Maricopa Agricultural Center, Arizona 85138.
authors.forename: John
authors.initials: J
authors.lastname: Heun
authors.affiliation: Arid-Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Maricopa, Arizona 85138.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Hunsaker
authors.affiliation: Department of Crop Sciences, University of Illinois, Urbana, Illinois 61801.
authors.forename: Alexander E
authors.initials: AE
authors.lastname: Lipka
authors.affiliation: Soil and Crop Sciences Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853.
authors.forename: Tim L
authors.initials: TL
authors.lastname: Setter
authors.affiliation: Arid-Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Maricopa, Arizona 85138.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Strand
authors.affiliation: Arid-Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Maricopa, Arizona 85138.
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Thorp
authors.affiliation: School of Plant Sciences, University of Arizona, Maricopa Agricultural Center, Arizona 85138.
authors.forename: Sam
authors.initials: S
authors.lastname: Wang
authors.affiliation: Arid-Land Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Maricopa, Arizona 85138.
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: White
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853 mag87@cornell.edu.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gore
mesh_terms: D000465:Algorithms; D002874:Chromosome Mapping; D016000:Cluster Analysis; D056726:Genetic Association Studies; D008040:Genetic Linkage; D003368:Gossypium / Q000235:genetics*; D008957:Models, Genetic; D015233:Models, Statistical; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D013312:Stress, Physiological / Q000235:genetics*
keywords: NDVI; QTL; canopy temperature; field-based HTPP; stress response
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