title: Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae.
abstract: Monochamus alternatus Hope is the main vector in China of the Pine Wilt Disease caused by the pine wood nematode Bursaphelenchus xylophilus. Although chemical control is traditionally used to prevent pine wilt disease, new strategies based in biological control are promising ways for the management of the disease. However, there is no deep sequence analysis of Monochamus alternatus Hope that describes the transcriptome and no information is available about gene function of this insect vector. We used next generation sequencing technology to sequence the whole fourth instar larva transcriptome of Monochamus alternatus Hope and successfully built a Monochamus alternatus Hope transcriptome database. In total, 105,612 unigenes were assigned for Gene Ontology (GO) terms, information for 16,730 classified unigenes was obtained in the Clusters of Orthologous Groups (COGs) database, and 13,024 unigenes matched with 224 predicted pathways in the Kyoto Encyclopedia of Genes and Genome (KEGG). In addition, genes related to putative insecticide resistance-related genes, RNAi, the Bt receptor, intestinal digestive enzymes, possible future insect control targets and immune-related molecules are described. This study provides valuable basic information that can be used as a gateway to develop new molecular tools for Monochamus alternatus Hope control strategies.
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Songqing
authors.initials: S
authors.lastname: Wu
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Ensi
authors.initials: E
authors.lastname: Shao
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, 92697, United States of America.
authors.forename: Carballar-Lejarazú
authors.initials: CL
authors.lastname: Rebeca
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Yueting
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Yani
authors.initials: Y
authors.lastname: Mou
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Runxue
authors.initials: R
authors.lastname: Xu
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Liang
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Shuangquan
authors.initials: S
authors.lastname: Zou
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.|Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
authors.affiliation: College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
authors.forename: Feiping
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D019476:Insect Proteins / Q000235:genetics; D007303:Insect Vectors / Q000235:genetics*; D007305:Insecticide Resistance; D007814:Larva / Q000235:genetics; D028223:Pinus / Q000469:parasitology*; D010935:Plant Diseases / Q000209:etiology / Q000469:parasitology*; D059467:Transcriptome; D017187:Tylenchida / Q000502:physiology*
chemical_list: D019476:Insect Proteins
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