title: 3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012.
abstract: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) was identified in 2012 as the causative agent of a severe, lethal respiratory disease occurring across several countries in the Middle East. To date there have been over 1600 laboratory confirmed cases of MERS-CoV in 26 countries with a case fatality rate of 36%. Given the endemic region, it is possible that MERS-CoV could spread during the annual Hajj pilgrimage, necessitating countermeasure development. In this report, we describe the clinical and radiographic changes of rhesus monkeys following infection with 5×10(6) PFU MERS-CoV Jordan-n3/2012. Two groups of NHPs were treated with either a human anti-MERS monoclonal antibody 3B11-N or E410-N, an anti-HIV antibody. MERS-CoV Jordan-n3/2012 infection resulted in quantifiable changes by computed tomography, but limited other clinical signs of disease. 3B11-N treated subjects developed significantly reduced lung pathology when compared to infected, untreated subjects, indicating that this antibody may be a suitable MERS-CoV treatment.
authors.affiliation: Emerging Viral Pathogens Section National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA. Electronic address: johnsonreed@mail.nih.gov.
authors.forename: Reed F
authors.initials: RF
authors.lastname: Johnson
authors.affiliation: Center for Infectious Disease Imaging, National Institutes of Health Clinical Center, Bethesda MD 20892, USA; Center for Research in Computer Vision (CRCV), Department of Electrics Electronics and Computer Science, University of Central Florida, Orlando, FL 32816, USA.
authors.forename: Ulas
authors.initials: U
authors.lastname: Bagci
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Keith
authors.affiliation: Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Tang
authors.affiliation: Center for Infectious Disease Imaging, National Institutes of Health Clinical Center, Bethesda MD 20892, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Mollura
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Larry
authors.initials: L
authors.lastname: Zeitlin
authors.affiliation: Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Qin
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Louis
authors.initials: L
authors.lastname: Huzella
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Bartos
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Natasha
authors.initials: N
authors.lastname: Bohorova
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Ognian
authors.initials: O
authors.lastname: Bohorov
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Goodman
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Do H
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Paulty
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Jesus
authors.initials: J
authors.lastname: Velasco
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego CA 92121, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Whaley
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Johnson
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: James
authors.initials: J
authors.lastname: Pettitt
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Britini L
authors.initials: BL
authors.lastname: Ork
authors.affiliation: Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research,Frederick, MD 21702-USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Solomon
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Oberlander
authors.affiliation: Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Jiusong
authors.initials: J
authors.lastname: Sun
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Holbrook
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Gene G
authors.initials: GG
authors.lastname: Olinger
authors.affiliation: Department of Microbiology and Immunology, Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Baric
authors.affiliation: Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Hensley
authors.affiliation: Emerging Viral Pathogens Section National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Jahrling
authors.affiliation: Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Marasco
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000914:Antibodies, Viral / Q000008:administration & dosage*; D018352:Coronavirus Infections / Q000188:drug therapy* / Q000473:pathology* / Q000821:virology; D004195:Disease Models, Animal; D006801:Humans; D008168:Lung / Q000473:pathology* / Q000821:virology; D008253:Macaca mulatta; D008297:Male; D065207:Middle East Respiratory Syndrome Coronavirus / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
keywords: Animal model, MERS; Antibody therapy; Human monoclonal antibody therapy; MERS-CoV; Respiratory syndrome
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