title: miR-708/LSD1 axis regulates the proliferation and invasion of breast cancer cells.
abstract: Breast cancer is one of the most common malignant tumors in women worldwide. The microRNAs (miRNAs) are small, noncoding RNAs that regulate various biological processes, including breast cancer. miR-708 played an important role in a variety of cancers. However, its involvement in breast cancer remains largely unclear. In this study, we found that forced the expression of miR-708 in breast cancer cell lines decreased cell proliferation and invasion, whereas inhibition of miR-708 increased cell growth and invasion. miR-708 could directly target the LSD1 3'UTR to downregulate the expression. Further studies suggested that inhibition of LSD1 could phenocopied function of the miR-708 overexpression in MDA-MB-231 cells .Overexpression of LSD1 could counteract the effects of miR-708 on the proliferation and invasion. Taken together, the results indicate that miR-708 may function as a tumor suppressor gene in breast cancer development, and miR-708/LSD1 axis may be a therapeutic intervention in breast cancer in the future.
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University, School of Medicine, Shanghai, 200065, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Oncology, The Center Hospital of Zaozhuang Mining Group, Zaozhuang, 277000, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Ma
authors.affiliation: Vocational College of Zaozhuang, Zaozhuang, 277000, China.
authors.forename: Guimei
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou City, Henan Province, 450007, China.
authors.forename: Longqiu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Oncology, The Center Hospital of Zaozhuang Mining Group, Zaozhuang, 277000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Center Hospital of Zaozhuang Mining Group, Zaozhuang, 277000, China.
authors.forename: Qingting
authors.initials: Q
authors.lastname: Yi
authors.affiliation: Department of Oncology, The Center Hospital of Zaozhuang Mining Group, Zaozhuang, 277000, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Cheng
mesh_terms: D020413:3' Untranslated Regions; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056466:Histone Demethylases / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C568528:MIRN708 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D056466:Histone Demethylases; C495195:KDM1A protein, human
keywords: Breast cancer; LSD1; invasion; miR-708; proliferation
references.citation: Nature. 2007 Oct 11;449(7163):682-8
references.pmid: 17898713
references.citation: PLoS One. 2015 Feb 13;10(2):e0118002
references.pmid: 25679396
references.citation: Cell. 2004 Dec 29;119(7):941-53
references.pmid: 15620353
references.citation: Cancer Med. 2016 Apr;5(4):684-92
references.pmid: 26833707
references.citation: Cell Rep. 2013 Oct 17;5(1):224-36
references.pmid: 24075993
references.citation: Oncol Rep. 2013 Aug;30(2):870-6
references.pmid: 23754151
references.citation: Carcinogenesis. 2010 Mar;31(3):512-20
references.pmid: 20042638
references.citation: Cell. 2009 Aug 21;138(4):660-72
references.pmid: 19703393
references.citation: Oncol Lett. 2014 Oct;8(4):1768-1774
references.pmid: 25202407
references.citation: Int J Cancer. 2011 Feb 1;128(3):574-86
references.pmid: 20333681
references.citation: Int J Cancer. 2015 Mar 1;136(5):E359-86
references.pmid: 25220842
references.citation: Cancer Res. 2013 Nov 15;73(22):6554-62
references.pmid: 24204025
references.citation: Cancer Res. 2011 Nov 1;71(21):6899-908
references.pmid: 21937684
references.citation: J Biol Chem. 2008 Nov 7;283(45):31079-86
references.pmid: 18790736
references.citation: Cancer Res. 2011 Oct 1;71(19):6208-19
references.pmid: 21852381
references.citation: Amino Acids. 2012 Feb;42(2-3):887-98
references.pmid: 21805138
references.citation: RNA. 2008 Nov;14(11):2348-60
references.pmid: 18812439
references.citation: Cell. 1993 Dec 3;75(5):843-54
references.pmid: 8252621
references.citation: Oncogene. 2008 Sep 18;27(42):5643-7
references.pmid: 18504431
references.citation: Nature. 2005 Sep 15;437(7057):436-9
references.pmid: 16079795
references.citation: Cancer Res. 2006 Aug 1;66(15):7390-4
references.pmid: 16885332
references.citation: Oncogenesis. 2015 Jun 15;4:e158
references.pmid: 26075749
references.citation: Biochim Biophys Acta. 2013 May;1829(5):480-6
references.pmid: 23507259
references.citation: Clin Cancer Res. 2012 Jul 1;18(13):3658-67
references.pmid: 22573352
references.citation: J Biol Chem. 2008 Jan 11;283(2):1026-33
references.pmid: 17991735
references.citation: Cancer Res. 2010 Apr 15;70(8):3119-27
references.pmid: 20354188
references.citation: J Cancer Res Clin Oncol. 2013 Jul;139(7):1189-98
references.pmid: 23568547
references.citation: Carcinogenesis. 2013 Jun;34(6):1196-207
references.pmid: 23354309
references.citation: Biochem Biophys Res Commun. 2012 Oct 19;427(2):336-42
references.pmid: 23000163
references.citation: Stem Cell Reports. 2014 Dec 9;3(6):1103-17
references.pmid: 25458897
references.citation: Clin Sci (Lond). 2015 Aug;129(4):305-17
references.pmid: 25728779
references.citation: Cancer Res. 2012 Jul 15;72(14):3618-30
references.pmid: 22552290
references.citation: Nat Rev Genet. 2009 Oct;10(10):704-14
references.pmid: 19763153
references.citation: J Bone Miner Res. 2001 Aug;16(8):1486-95
references.pmid: 11499871
references.citation: Cancer Res. 2007 Nov 15;67(22):11001-11
references.pmid: 18006846
references.citation: Biomed Pharmacother. 2015 Jul;73:154-9
references.pmid: 26211597
references.citation: Mol Cancer Ther. 2008 Jul;7(7):2152-9
references.pmid: 18645025
references.citation: BMC Cancer. 2014 Aug 20;14:604
references.pmid: 25139823
references.citation: Breast Cancer Res. 2012 Sep 17;14(5):112
references.pmid: 22992372