title: [Epidemiologic and etiologic characteristics of diarrheagenic Escherichia coli infection in population in Shenzhen].
abstract: OBJECTIVE
  To understand the epidemiologic and etiologic characteristics of diarrheagenic Escherichia (E.) coli infections in Shenzhen.
  
  
  METHODS
  Stool samples were collected from acute diarrheal patients in four sentinel hospitals in Shenzhen and diarrheagenic E. coli strains were isolated and identified with multiplex real-time PCR. Serotyping and pulsed-field gel electrophoresis (PFGE) typing were conducted for the diarrheagenic E. coli isolates.
  
  
  RESULTS
  A total of 74 diarrheagenic E. coli strains were isolated from 1 823 stool samples (4.06%). The patients were mainly young children aged <3 years and adults aged 20-39 years, and the infections mainly occurred during May-September of a year. Enterotoxigenic E. coli (ETEC) and enteropathognic E. coli (EPEC) were predominant (45.9% and 31.1%). Serogroups and PFGE patterns varied among the diarrheagenic E. coli isolates. However, serogroup O159 were predominant in ETEC and there were 5 clusters with ≥2 strains sharing same PFGE patterns.
  
  
  CONCLUSIONS
  ETEC and EPEC were predominant in diarrheagenic E. coli strains isolated from diarrheal patients in Shenzhen. Age and season specific characteristics of diarrheagenic E. coli infections were observed. The serotypes and PFGE patterns of diarrheagenic E. coli strains varied. Close attention should be paid to the possible ETEC outbreak.
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Huixia
authors.initials: H
authors.lastname: Xian
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Yiman
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Qiongcheng
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Shenzhen University, Shenzhen 518060, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Hu
authors.affiliation: Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China; School of Life Sciences, Shenzhen University, Shenzhen 518060, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000328:Adult; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D054307:Enterotoxigenic Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D012703:Serotyping; D055815:Young Adult