title: RNA biochemistry. Transcriptome-wide distribution and function of RNA hydroxymethylcytosine.
abstract: Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts of many genes, notably in coding sequences, and identify consensus sites for hydroxymethylation. We found that RNA hydroxymethylation can favor mRNA translation. Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Delatte
authors.affiliation: Waksman Institute, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Biology of the Gene, Faculty of Sciences, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Long Vo
authors.initials: LV
authors.lastname: Ngoc
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Collignon
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Elise
authors.initials: E
authors.lastname: Bonvin
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Rachel
authors.initials: R
authors.lastname: Deplus
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Emilie
authors.initials: E
authors.lastname: Calonne
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Bouchra
authors.initials: B
authors.lastname: Hassabi
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Pascale
authors.initials: P
authors.lastname: Putmans
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Awe
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA.
authors.forename: Collin
authors.initials: C
authors.lastname: Wetzel
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Kreher
authors.affiliation: Laboratory of Molecular Biology of the Gene, Faculty of Sciences, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Romuald
authors.initials: R
authors.lastname: Soin
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Creppe
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA.
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Limbach
authors.affiliation: Laboratory of Molecular Biology of the Gene, Faculty of Sciences, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Cyril
authors.initials: C
authors.lastname: Gueydan
authors.affiliation: Laboratory of Molecular Biology of the Gene, Faculty of Sciences, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Véronique
authors.initials: V
authors.lastname: Kruys
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Brehm
authors.affiliation: Waksman Institute, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Minakhina
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Defrance
authors.affiliation: Waksman Institute, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
authors.forename: Ruth
authors.initials: R
authors.lastname: Steward
authors.affiliation: Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Université Libre de Bruxelles (ULB), Brussels, Belgium. ffuks@ulb.ac.be.
authors.forename: François
authors.initials: F
authors.lastname: Fuks
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D000818:Animals; D001921:Brain / Q000002:abnormalities* / Q000378:metabolism; D002460:Cell Line; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008745:Methylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D049308:Dioxygenases