title: Polyphenols and Polypeptides in Chinese Rice Wine Inhibit Homocysteine-induced Proliferation and Migration of Vascular Smooth Muscle Cells.
abstract: The beneficial effect of Chinese rice wine on atherosclerosis has been proved, but the exact components that have the cardiovascular protective effect are still unknown. This study aimed to explore the exact ingredients in Chinese rice wine that could inhibit homocysteine (Hcy)-induced vascular smooth muscle cell (VSMC) proliferation and migration. VSMCs were divided into 7 groups: control, Hcy (1 mmol/L), Hcy + oligosaccharide, Hcy + polypeptides, Hcy + polyphenols, Hcy + alcohol, and Hcy + Chinese rice wine. methyl thiazolyl tetrazolium (MTT) assay, Transwell chambers, and wound-healing assay were used to test the proliferation and migratory ability of the VSMCs. Western blot and gelatin zymography were used to investigate the expressions and activities of metal matrix proteinase 2/9 (MMP-2/9) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in VSMCs. Polypeptides and polyphenols in the Chinese rice wine reduced the proliferation and migration ability of the VSMCs. Furthermore, they also decreased the expression and activity of MMP-2/9 but had no obvious impact on the expression of TIMP-2 in each group. This study further confirms that polypeptides and polyphenols in the Chinese rice wine could inhibit Hcy-induced proliferation and migration of VSMCs and maintain the balance between matrix metalloproteinases (MMPs) and TIMPs.
authors.affiliation: *Department of Cardiology, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing, China; and †The First Clinical Medical College, Wenzhou Medical University, Wenzhou, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Meng
authors.forename: Longbin
authors.initials: L
authors.lastname: Liu
authors.forename: Changzuan
authors.initials: C
authors.lastname: Zhou
authors.forename: Sunlei
authors.initials: S
authors.lastname: Pan
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Zhai
authors.forename: Chengjian
authors.initials: C
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ji
authors.forename: Jufang
authors.initials: J
authors.lastname: Chi
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
authors.forename: Hangyuan
authors.initials: H
authors.lastname: Guo
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D006710:Homocysteine / Q000037:antagonists & inhibitors*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009844:Oligosaccharides; D012275:Oryza; D010455:Peptides / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis; D014920:Wine
chemical_list: D009844:Oligosaccharides; D010455:Peptides; D059808:Polyphenols; D006710:Homocysteine; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9