title: Protective effect of ferulic acid against 2,2'-azobis(2-amidinopropane) dihydrochloride-induced oxidative stress in PC12 cells.
abstract: Oxidative stress is closely related to the pathogenesis of neurodegenerative disorders such as Parkinson's disease and Alzheimer's disease. However, the underlying antioxidant mechanisms of ferulic acid (FA) aganist oxidantive stress are poorly understood. We evaluated the potential protective effects of FA against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced damage in PC12 cells. Our results indicated that pretreatment with FA prior to AAPH exposure significantly increased PC12 cell survival, and also increased catalase and superoxide dismutase activity. Furthermore, FA treatment reduced cellular lactate dehydrogenase release and malondialdehyde levels. It attenuated AAPH-induced apoptosis in PC12 cells, as determined by flow cytometric detection of annexin V. Reductions in mitochondrial membrane potential and accumulation of intracellular Ca2+ were also inhibited by FA treatment. These findings suggested that FA protected PC12 cells against AAPH-induced oxidative stress, and may be a neuroprotective agent.
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China zhanghuigwzx@gmail.com.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: X
authors.initials: X
authors.lastname: Miao
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Jiangnan University State Key Laboratory of Food Science and Technology, School of Food Science and Technology Wuxi China.
authors.forename: X
authors.initials: X
authors.lastname: Qi
mesh_terms: D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D019636:Neurodegenerative Diseases / Q000188:drug therapy / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000578:Amidines; D017304:Annexin A5; D000975:Antioxidants; D003373:Coumaric Acids; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; C046728:2,2'-azobis(2-amidinopropane); C004999:ferulic acid; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D002118:Calcium