title: Coupling Electrochemistry with Probe Electrospray Ionization Mass Spectrometry.
abstract: A new coupling of electrochemistry with mass spectrometry (MS) using probe electrospray ionization (PESI) is presented. Due to the high salt tolerance of PESI, the detection of electrochemical reaction products in room-temperature ionic liquids (RTILs) is realized for the first time. Furthermore, PESI-MS allows the analysis of electrochemical reaction products on different or multiple electrode surfaces. In addition, peptides and proteins fractionated through isoelectric focusing (IEF) in the presence of an external electric field can also be directly analyzed by using PESI-MS, suggesting a new and rapid characterization means for the IEF technique. This study reveals the versatility of EC/PESI-MS, which could have an impact in electrochemistry and bioanalysis fields.
authors.affiliation: Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Liu
authors.affiliation: Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Held
authors.affiliation: Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA.
authors.forename: Howard D
authors.initials: HD
authors.lastname: Dewald
authors.affiliation: Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA. chenh2@ohio.edu.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
mesh_terms: D055664:Electrochemical Techniques; D004566:Electrodes; D052578:Ionic Liquids / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013499:Surface Properties
chemical_list: D052578:Ionic Liquids
keywords: analytical methods; electrochemical reactions; ionic liquids; mass spectrometry; probe electrospray ionization