title: Cobalt chloride-mediated protein kinase Cα (PKCα) phosphorylation induces hypoxia-inducible factor 1α (HIF1α) in the nucleus of gastric cancer cell.
abstract: Hypoxia promotes cancer progression, and metastasis. The major protein expressed in hypoxic solid cancer is hypoxia-inducible factor 1 (HIF1). We show that enhanced phosphorylation of a conventional protein kinase C isoform, PKCα, at threonine 638 (T(638)) by hypoxia-mimetic cobalt chloride induces HIF1α in nuclei of gastric epithelial cells (GECs). Moreover, phospho-T(638)-PKCα (P-PKCα) interacts with p300-HIF1α complex in the nuclei of hypoxic GECs and PKCα phosphorylation at T(638) enhances transcriptional activity of HIF1α. High P-PKCα expression in neoplastic gastric cancer biopsy samples as compared to nonneoplastic samples suggests that P-PKCα might act as an indicator of gastric cancer progression.
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Suvasmita
authors.initials: S
authors.lastname: Rath
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Anand
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Nilabh
authors.initials: N
authors.lastname: Ghosh
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Lopamudra
authors.initials: L
authors.lastname: Das
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Shrikant B
authors.initials: SB
authors.lastname: Kokate
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Pragyesh
authors.initials: P
authors.lastname: Dixit
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India.
authors.forename: Swetapadma
authors.initials: S
authors.lastname: Majhi
authors.affiliation: Department of Oncopathology, Acharya Harihar Regional Cancer Centre, Cuttack, 753007, Odisha, India.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Rout
authors.affiliation: Department of Gastroenterology, SCB Medical College, Cuttack, 753007, Odisha, India.
authors.forename: Shivaram P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: National Institute of Science Education and Research (NISER), School of Biological Sciences, PO. Bhimpur-Padanpur, Via- Jatni, Dist. Khurda, 752050, Odisha, India. Electronic address: asima@niser.ac.in.
authors.forename: Asima
authors.initials: A
authors.lastname: Bhattacharyya
mesh_terms: D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D003035:Cobalt / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects / Q000502:physiology; D051571:Protein Kinase C-alpha / Q000378:metabolism*
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D003035:Cobalt; D051571:Protein Kinase C-alpha; C018021:cobaltous chloride
keywords: Cobalt chloride; Gastric epithelial cell; HIF1α; Hypoxia; PKC; p300