title: Selective Precipitation of Proteins.
abstract: Selective precipitation of proteins can be used as a bulk method to recover the majority of proteins from a crude lysate, as a selective method to fractionate a subset of proteins from a protein solution, or as a very specific method to recover a single protein of interest from a purification step. This unit describes a number of methods suitable for selective precipitation. In each of the protocols that are outlined, the physical or chemical basis of the precipitation process, the parameters that can be varied for optimization, and the basic steps for developing an optimized precipitation are described.
authors.affiliation: Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.
authors.forename: Daumantas
authors.initials: D
authors.lastname: Matulis
mesh_terms: D005590:Fractional Precipitation / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility
chemical_list: D011506:Proteins
keywords: coprecipitation; matrix ligand; protein precipitation; protein purification; protein stability; salting out
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