title: Combined identification of long non-coding RNA CCAT1 and HOTAIR in serum as an effective screening for colorectal carcinoma.
abstract: Long non-coding RNAs (lncRNAs) CCAT1 and HOTAIR have been shown to play an important regulatory role in cancer biology, and CCAT1 and HOTAIR are upregulated in several cancers, however, its value in the diagnosis of colorectal cancer (CRC) is unclear. Therefore, the aim of this study is to evaluate the clinical significance of plasma CCAT1 and HOTAIR as a biomarker in the screening of CRC. In our study, we found that the levels of HOTAIR (P < 0.05) and CCAT1 (P < 0.05) were significantly higher in plasma of CRC patients than that of the healthy control. Moreover, the levels of lincRNA-p21 (P < 0.05) were obviously decreased in plasma of CRC patients as compared to those of healthy control. There was highly correlated for CCAT1 (R = 0.752, mean differences = -0.06 ± 1.20), HOTAIR (R = 0.739, mean differences = -0.26 ± 0.76) and lincRNA-p21 (R = 0.848, mean differences = -0.41 ± 0.89) in plasma and serum. By receiver operating characteristic curve (ROC) analysis, plasma CCAT1 provided the higher diagnostic performance for detection of CRC (the area under the ROC curve (AUC), 0.836; P < 0.001; sensitivity, 75.7%; specificity, 85.3%). Moreover, CCAT1 combining with HOTAIR could provide a more effective diagnosis performance (AUC, 0.954, P < 0.001, sensitivity, 84.3%; specificity, 80.2%). Most importantly, this combination was effective to detect CRC at an early stage (85%). In conclusion, our results demonstrated that increased plasma HOTAIR and CCAT1 could be used as a predictive biomarker for CRC screening, and that combination of HOTAIR and CCAT1 had a higher positive diagnostic rate of CRC than HOTAIR or CCAT1 alone.
authors.affiliation: Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Xinjiang Medical University Urumuqi 830011, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Oncological Surgery, The Second Affiliated Hospital of Xinjiang Medical University Urumqi 830019, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Song
authors.affiliation: Emergency Center of The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oncological Surgery, The Second Affiliated Hospital of Xinjiang Medical University Urumqi 830019, China.
authors.forename: Mulati
authors.initials: M
authors.lastname: Kuerban
authors.affiliation: Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Xinjiang Medical University Urumuqi 830011, China.
authors.forename: Haijiang
authors.initials: H
authors.lastname: Wang
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002277:Carcinoma / Q000097:blood* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D055088:Early Detection of Cancer / Q000379:methods; D006801:Humans; D053208:Kaplan-Meier Estimate; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C579842:CCAT1 long noncoding RNA, human; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding
keywords: CCAT1; Colorectal carcinoma; HOTAIR; long non-coding RNA; tumor biomarker
references.citation: Oncotarget. 2015 Sep 8;6(26):22513-25
references.pmid: 26068968
references.citation: Mol Cancer. 2011;10:38
references.pmid: 21489289
references.citation: BMC Cancer. 2013;13:543
references.pmid: 24215576
references.citation: J Gene Med. 2005 Mar;7(3):366-74
references.pmid: 15521051
references.citation: Mol Carcinog. 2015 Sep;54(9):742-50
references.pmid: 24519959
references.citation: Anticancer Res. 2013 Aug;33(8):3185-93
references.pmid: 23898077
references.citation: J Clin Endocrinol Metab. 2003 Nov;88(11):5119-26
references.pmid: 14602737
references.citation: Tumour Biol. 2015 Mar;36(3):2007-12
references.pmid: 25391424
references.citation: Int J Oncol. 2012 Jul;41(1):276-84
references.pmid: 22576688
references.citation: J Cancer Res Ther. 2014 Dec;10 Suppl:307-9
references.pmid: 25693941
references.citation: Asian Pac J Cancer Prev. 2014;15(19):8455-60
references.pmid: 25339046
references.citation: Tumour Biol. 2014 Dec;35(12):12181-8
references.pmid: 25185650
references.citation: Anticancer Res. 2015 Mar;35(3):1385-8
references.pmid: 25750289
references.citation: Cell Res. 2014 May;24(5):513-31
references.pmid: 24662484
references.citation: Tumour Biol. 2015 Sep;36(9):7175-83
references.pmid: 25894381
references.citation: Eur J Cancer. 2013 Sep;49(13):2949-59
references.pmid: 23726266
references.citation: Mol Cell. 2013 Mar 28;49(6):1083-96
references.pmid: 23395002
references.citation: Clin Lab. 2014;60(3):397-405
references.pmid: 24697115
references.citation: Clin Colorectal Cancer. 2013 Dec;12(4):261-6
references.pmid: 24012455
references.citation: Mod Pathol. 2013 Feb;26(2):155-65
references.pmid: 22996375
references.citation: Cancer. 2014 Nov 1;120(21):3320-8
references.pmid: 24986041
references.citation: Genes Cancer. 2011 Aug;2(8):829-40
references.pmid: 22393467
references.citation: Sci Rep. 2015;5:11516
references.pmid: 26096073
references.citation: Breast Cancer Res Treat. 2015 Jul;152(1):199-208
references.pmid: 26033707
references.citation: Eur Urol. 2014 Jun;65(6):1140-51
references.pmid: 24373479
references.citation: Oncol Rep. 2014 Apr;31(4):1839-45
references.pmid: 24573322
references.citation: Br J Cancer. 2013 Jun 25;108(12):2419-25
references.pmid: 23660942
references.citation: Nat Genet. 2007 Aug;39(8):989-94
references.pmid: 17618283
references.citation: Mol Cancer. 2015;14:3
references.pmid: 25608466
references.citation: Biochim Biophys Acta. 2014 Oct;1842(10):1910-1922
references.pmid: 24667321
references.citation: Eur J Gastroenterol Hepatol. 2009 Jun;21(6):688-92
references.pmid: 19445043
references.citation: Oncotarget. 2014 Nov 30;5(22):11091-102
references.pmid: 25526029
references.citation: Medicine (Baltimore). 2014 Dec;93(28):e303
references.pmid: 25526476
references.citation: Int J Cancer. 2012 Apr 1;130(7):1598-606
references.pmid: 21547902