title: Pneumococcus with the "6E" cps Locus Produces Serotype 6B Capsular Polysaccharide.
abstract: Genetic studies of serogroup 6 isolates ofStreptococcus pneumoniaeidentified putative serotype 6E. Although its capsular polysaccharide structure has not been elucidated, putative serotype 6E is described in an increasing number of studies as a potentially new serotype. We show here that SPEC6B, which is widely used as a target strain for serotype 6B opsonophagocytosis assays, has the genetic features of the putative serotype 6E but produces capsular polysaccharide identical to 6B capsular polysaccharide as determined by one-dimensional (1D) and 2D nuclear magnetic resonance (NMR). Thus, putative serotype 6E is a mere genetic variant of serotype 6B. Also, SPEC6B is appropriate as a target strain for serotype 6B opsonophagocytosis assays. This example illustrates the difficulties of assigning new bacterial serotypes based on genetic findings alone.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Burton
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: K Aaron
authors.initials: KA
authors.lastname: Geno
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Jamil S
authors.initials: JS
authors.lastname: Saad
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA nahm@uab.edu.
authors.forename: Moon H
authors.initials: MH
authors.lastname: Nahm
mesh_terms: D005838:Genotype; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry*; D065288:Serogroup; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial
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