title: Southern blight disease of tomato control by 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing Paenibacillus lentimorbus B-30488.
abstract: Tomato cultivation is highly susceptible for soil born diseases and among them southern blight disease caused by Scelerotium rolfsii is very common. For its management use of chemical fungicides is not very successful as their spores are able to survive for many years in the soil. As an alternative eco-friendly approach to control the disease antagonistic microbes are being characterized.Among them plant growth promoting rhizobacteria Paenibacillus lentimorbus B-30488 (B-30488) with antagonistic properties, multiple PGP attributes stress tolerance and ACC deaminase enzyme activity is characterized to decipher its mode of action against S. rolfsii under in vitro and in vivo conditions. In vitro results obtained from this study clearly demonstrate that B-30488 has ability to show antagonistic properties under different abiotic stresses against S. rolfsii. Similar results were also obtained from in vivo experiments where B-30488 inoculation has efficiently controlled the disease caused by S. rolfsii and improve the plant growth. Deleterious enhanced ethylene level in S. rolfsii infected plants was also ameliorated by inoculation of ACC deaminase producing B-30488. The ACC accumulation, ACO and ACS activities were also modulated in S. rolfsii infected plants. Results from defense enzymes and other biochemical attributes were also support the role of B-30488 inoculation in ameliorating the biotic stress caused by S. rolfsii in tomato plants. These results were further validated by pathogen related gene expression analysis by real time PCR. Overall results from the present study may be concluded that ACC deaminase producing B-30488 has ability to control the southern blight disease caused by S. rolfsii and commercial bioinoculant package may be developed.
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Ritu
authors.initials: R
authors.lastname: Dixit
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Lalit
authors.initials: L
authors.lastname: Agrawal
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Swati
authors.initials: S
authors.lastname: Gupta
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Yadav
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Puneet Singh
authors.initials: PS
authors.lastname: Chauhan
authors.affiliation: a CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Nautiyal
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001487:Basidiomycota; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D015227:Lipid Peroxidation; D018551:Solanum lycopersicum / Q000378:metabolism / Q000382:microbiology*; D056507:Paenibacillus / Q000201:enzymology*; D009195:Peroxidase / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D011392:Proline / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005030:Ethylenes; D002734:Chlorophyll; C036216:ethylene; D011392:Proline; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: ACC deaminase; Paenibacillus lentimorbus; Scelerotium rolfsii; biocontrol; biotic stress
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