title: Biomolecular functionalization for enhanced cell-material interactions of poly(methyl methacrylate) surfaces.
abstract: The integration of implants or medical devices into the body tissues requires of good cell-material interactions. However, most polymeric materials used for these applications lack on biological cues, which enhanced mid- and long-term implant failure due to weak integration with the surrounding tissue. Commonly used strategies for tissue-material integration focus on functionalization of the material surface by means of natural proteins or short peptides. However, the use of these biomolecules involves major drawbacks such as immunogenic problems and oversimplification of the constructs. Here, designed elastin-like recombinamers (ELRs) are used to enhance poly(methyl methacrylate) surface properties and compared against the use of short peptides. In this study, cell response has been analysed for different functionalization conditions in the presence and absence of a competing protein, which interferes on surface-cell interaction by unspecific adsorption on the interface. The study has shown that ELRs can induce higher rates of cell attachment and stronger cell anchorages than short peptides, being a better choice for surface functionalization.
authors.affiliation: Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona 08028, Spain,; CIBER en Bioingenería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Punet
authors.affiliation: Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona 08028, Spain.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Mauchauffé
authors.affiliation: CIBER en Bioingenería, Biomateriales y Nanomedicina (CIBER-BBN), Spain,; G.I.R. Bioforge, Universidad Valladolid (UVA), Valladolid 47011, Spain and.
authors.forename: José C
authors.initials: JC
authors.lastname: Rodríguez-Cabello
authors.affiliation: CIBER en Bioingenería, Biomateriales y Nanomedicina (CIBER-BBN), Spain,; G.I.R. Bioforge, Universidad Valladolid (UVA), Valladolid 47011, Spain and.
authors.forename: Matilde
authors.initials: M
authors.lastname: Alonso
authors.affiliation: Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona 08028, Spain,; CIBER en Bioingenería, Biomateriales y Nanomedicina (CIBER-BBN), Spain,; Department of Material Science and Metallurgical Engineering, Technical University of Catalonia (UPC), Barcelona 08028, Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engel
authors.affiliation: Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona 08028, Spain,; CIBER en Bioingenería, Biomateriales y Nanomedicina (CIBER-BBN), Spain,; Department of Material Science and Metallurgical Engineering, Technical University of Catalonia (UPC), Barcelona 08028, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Mateos-Timoneda
keywords: PMMA; RGD peptide; cell adhesion; elastin-like recombinamer; poly(methyl methacrylate); surface modification
references.citation: Biomaterials. 2011 Jun;32(18):4195-7
references.pmid: 21515166
references.citation: J Spinal Disord Tech. 2012 Apr;25(2):E28-35
references.pmid: 22454185
references.citation: J Colloid Interface Sci. 2014 Oct 1;431:1-7
references.pmid: 24972346
references.citation: Adv Drug Deliv Rev. 2010 Dec 30;62(15):1479-85
references.pmid: 20385185
references.citation: J Mater Sci Mater Med. 2004 Apr;15(4):479-84
references.pmid: 15332621
references.citation: J Oral Maxillofac Surg. 2013 Feb;71(2):e81-8
references.pmid: 23351772
references.citation: Biomaterials. 2007 Jul;28(20):3074-82
references.pmid: 17428532
references.citation: Acta Biomater. 2014 Jun;10(6):2824-33
references.pmid: 24525034
references.citation: Biomaterials. 2003 Nov;24(24):4385-415
references.pmid: 12922151
references.citation: J Mater Sci Mater Med. 1999 Dec;10(12):837-9
references.pmid: 15347961
references.citation: Colloids Surf B Biointerfaces. 2014 Feb 1;114:225-33
references.pmid: 24200950
references.citation: Biomaterials. 2011 Jun;32(18):4211-4
references.pmid: 21515169
references.citation: J Long Term Eff Med Implants. 2005;15(6):629-39
references.pmid: 16393131
references.citation: Biomacromolecules. 2013 Aug 12;14 (8):2690-702
references.pmid: 23805782
references.citation: J Prosthodont. 2009 Feb;18(2):106-11
references.pmid: 19254299
references.citation: Orthopade. 2003 Jan;32(1):41-50
references.pmid: 12557085
references.citation: J Biosci Bioeng. 2001;91(3):233-44
references.pmid: 16232982
references.citation: J Oral Maxillofac Surg. 2005 Jul;63(7):1048-51
references.pmid: 16003639
references.citation: Nat Mater. 2008 Oct;7(10):816-23
references.pmid: 18724374
references.citation: J Spinal Disord Tech. 2011 Aug;24(6):E49-56
references.pmid: 21685804
references.citation: Ann Plast Surg. 1991 Jan;26(1):57-63
references.pmid: 1994814
references.citation: Clin Oral Implants Res. 2013 Aug;24 Suppl A100:100-9
references.pmid: 22150934