title: Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.
abstract: Proteolysis has been cited as an important contributor to cancer initiation and progression. One can take advantage of tumor-associated proteases to selectively deliver imaging agents. Protease-activated imaging systems have been developed using substrates designed for hydrolysis by members of the matrix metalloproteinase (MMP) family. We presently describe approaches by which one can optically image matrix metalloproteinase activity implicated in breast cancer progression, with consideration of selective versus broad protease probes.
authors.affiliation: Department of Chemistry & Biochemistry, Florida Atlantic University, Jupiter, FL, 33458, USA. fieldsg@fau.edu.|Department of Chemistry, The Scripps Research Institute/Scripps Florida, Jupiter, FL, 33458, USA. fieldsg@fau.edu.|Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987, USA. fieldsg@fau.edu.
authors.forename: Gregg B
authors.initials: GB
authors.lastname: Fields
authors.affiliation: Department of Chemistry & Biochemistry, Florida Atlantic University, Jupiter, FL, 33458, USA.
authors.forename: Maciej J
authors.initials: MJ
authors.lastname: Stawikowski
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D006801:Humans; D020782:Matrix Metalloproteinases / Q000737:chemistry / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary
chemical_list: D010446:Peptide Fragments; D020782:Matrix Metalloproteinases
keywords: Collagen; Imaging agent; Matrix metalloproteinase; Near infrared; Triple-helix