title: TRPV6 plays a new role in predicting survival of patients with esophageal squamous cell carcinoma.
abstract: BACKGROUND
  TRPV6 is over-expressed and promotes the proliferation and invasion in many cancers. The association between the expression of TRPV6 and clinical outcome in esophageal squamous cell carcinoma (ESCC) has not been studied yet. We aim to elucidate the role of TRPV6 in predicting prognosis of patients with ESCC.
  
  
  METHODS
  In the retrospective study, mRNA level of TRPV6 was examined in patients (N = 174) from Sun Yat-sen University Cancer Center (mRNA cohort) and protein level of TRPV6 was examined in patients (N = 218) from Linzhou Cancer Hospital (protein cohort). Statistical analysis was performed to test the clinical and prognostic significance of TRPV6.
  
  
  RESULTS
  TRPV6 was down-regulated in ESCC tissues and cell lines. Patients with downregulation of TRPV6 trended to have a higher rate of advanced pT stage in both mRNA cohort (P = 0.089) and protein cohort (P = 0.073), though not statistically significant. No significant association was observed between TRPV6 expression and disease-specific survival (DSS) in both two cohorts. However, stratified survival analysis based on the gender showed that in mRNA cohort, downregulation of TRPV6 was associated with an unfavorable 3-year DSS in patients with male (47.3 % vs 63.6 %, P = 0.027) and with favorable 3-year DSS in patients with female (66.7 % vs 43.0 %, P = 0.031). The result was confirmed in protein cohort. Male patients with downregulation of TRPV6 had a poor 3-year DSS (20.0 % vs 57.1 %,P < 0.001) while female counterparts showed an enhanced 3-year DSS (56.1 % vs 28.6 %, P = 0.005).
  
  
  CONCLUSIONS
  TRPV6 is down-regulated in ESCC. As a predictive biomarker, TRPV6 plays a Janus-like role in predicting survival of male and female ESCC patients.
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. zhangshuish@163.com.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. zhangshuish@163.com.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. zhangshuish@163.com.|Department of Thoracic Surgery, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China. zhangshuish@163.com.
authors.forename: Shui-Shen
authors.initials: SS
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China. xiexuan@sysucc.org.cn.
authors.forename: Xuan
authors.initials: X
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. wenjing@sysucc.org.cn.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. wenjing@sysucc.org.cn.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. wenjing@sysucc.org.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. luokj@sysucc.org.cn.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. luokj@sysucc.org.cn.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. luokj@sysucc.org.cn.
authors.forename: Kong-Jia
authors.initials: KJ
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. liuqw@sysucc.org.cn.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. liuqw@sysucc.org.cn.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. liuqw@sysucc.org.cn.
authors.forename: Qian-Wen
authors.initials: QW
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. hongyang@sysucc.org.cn.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. hongyang@sysucc.org.cn.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. hongyang@sysucc.org.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. huyi@sysucc.org.cn.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. huyi@sysucc.org.cn.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. huyi@sysucc.org.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China. fu_jianhua@126.com.|Guangdong Esophageal Cancer Research Institute, Guangzhou, People's Republic of China. fu_jianhua@126.com.|Department of Thoracic Oncology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People's Republic of China. fu_jianhua@126.com.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Fu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D002681:China; D018572:Disease-Free Survival; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics; D012189:Retrospective Studies; D012307:Risk Factors; D012737:Sex Factors; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D015220:Calcium Channels; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C422827:TRPV6 protein, human
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