title: Influence of Melaleuca alternifolia oil nanoparticles on aspects of Pseudomonas aeruginosa biofilm.
abstract: The Pseudomonas aeruginosa is a gram-negative bacillus and frequent cause of infection. This microorganism is resistant intrinsically to various drugs. The P. aeruginosa is associated with the biofilm formation, which causes worsen the prognosis and difficulty the treatment. The influence of Melaleuca alternifolia oil or "tree of tee" oil (TTO) and TTO nanoparticles on adhesion of P. aeruginosa in buccal epithelial cells was investigated. Also was determined the antimicrobial and antibiofilm activity against this microorganism. The TTO nanoparticles were produced by deposition of preformed polymer and the physic-chemical properties of nanoparticles were measured by electrophoresis and dynamic light scattering. The characterization of nanoparticle showed acceptable values for diameter and zeta potential. The evaluation of antimicrobial and antibiofilm activity against P. aeruginosa PAO1 was performed by microdilution indicating the minimal inhibitory concentration, and the potential antibiofilm. It was verified the action on virulence factors such the motility, besides the influence on adhesion in buccal epithelial cells. Both oil and nanoparticles showed a decrease in adhesion of microorganisms to buccal cells, decrease of biofilm and interfering on P. aeruginosa PAO1 motility. The nanostructuration of TTO, shows be a viable alternative against formed biofilm microorganisms.
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Comin
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil. Electronic address: leonardoquintanalopes@gmail.com.
authors.forename: Leonardo Q S
authors.initials: LQ
authors.lastname: Lopes
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Priscilla M
authors.initials: PM
authors.lastname: Quatrin
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Márcia E
authors.initials: ME
authors.lastname: de Souza
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Pauline C
authors.initials: PC
authors.lastname: Bonez
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Francieli G
authors.initials: FG
authors.lastname: Pintos
authors.affiliation: Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Renata P
authors.initials: RP
authors.lastname: Raffin
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Rodrigo de A
authors.initials: Rde A
authors.lastname: Vaucher
authors.affiliation: Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), 13084-970, Campinas-SP, Brazil.
authors.forename: Diego S T
authors.initials: DS
authors.lastname: Martinez
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil; Laboratory of Microbiology, Microbiology and Parasitology Department, Health Sciences Center, Universidade Federal de Santa Maria, Brazil.
authors.forename: Roberto C V
authors.initials: RC
authors.lastname: Santos
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D031650:Melaleuca / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009061:Mouth Mucosa / Q000382:microbiology; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D020947:Tea Tree Oil / Q000737:chemistry / Q000494:pharmacology*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D037521:Virulence Factors; D020947:Tea Tree Oil
keywords: Biofilm; Melaleuca alternifolia; Nanoparticles; Pseudomonas aeruginosa PAO1