title: Aβ 1-40 enhances the proliferation of human diploid fibroblasts.
abstract: There is a vast literature on the role of beta amyloid (Aβ) peptides in the pathogenesis of Alzheimer's disease. However, there is a paucity of research on the potential physiological functions of these evolutionarily conserved products of the Aβ precursor protein. Based on previous studies in neuroblastoma cells, we hypothesized that Aβ may contribute to the proliferation of somatic cells. We present evidence supporting this hypothesis for the case of cultured human skin fibroblasts immortalized with the catalytic subunit of human telomerase (hTERT). Optimal concentrations ranged from 100 pM-10 nM, depending on the nature of the assay.
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Theda
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA.
authors.forename: Michelle K
authors.initials: MK
authors.lastname: Drews
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA.
authors.forename: Galynn
authors.initials: G
authors.lastname: Zitnik
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA; Department of Medicine, Chiba University, Chiba, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Oshima
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA. Electronic address: gmmartin@u.washington.edu.
authors.forename: George M
authors.initials: GM
authors.lastname: Martin
mesh_terms: D016229:Amyloid beta-Peptides / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D010446:Peptide Fragments / Q000502:physiology*; D012867:Skin / Q000166:cytology; D019098:Telomerase
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D019098:Telomerase
keywords: Alzheimer's disease; Beta amyloid; Cell culture
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