title: Functional Characterization of a Dihydroflavanol 4-Reductase from the Fiber of Upland Cotton (Gossypium hirsutum).
abstract: Dihydroflavanol 4-reductase (DFR) is a key later enzyme involved in two polyphenols' (anthocyanins and proanthocyanidins (PAs)) biosynthesis, however it is not characterized in cotton yet. In present reports, a DFR cDNA homolog (designated as GhDFR1) was cloned from developing fibers of upland cotton. Silencing GhDFR1 in cotton by virus-induced gene silencing led to significant decrease in accumulation of anthocyanins and PAs. More interestingly, based on LC-MS analysis, two PA monomers, (-)-epicatachin and (-)-epigallocatachin, remarkably decreased in content in fibers of GhDFR1-silenced plants, but two new monomers, (-)-catachin and (-)-gallocatachin were present compared to the control plants infected with empty vector. The ectopic expression of GhDFR1 in an Arabidopsis TT3 mutant allowed for reconstruction of PAs biosynthesis pathway and led to accumulation of PAs in seed coat. Taken together, these data demonstrate that GhDFR1 contributes to the biosynthesis of anthocyanins and PAs in cotton.
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. wangle510@foxmail.com.|Hunan Provincial Key Laboratory of Plant Resources Conservation and Utilization, College of Biology and Environmental Sciences, Jishou University, No. 120 Ren Min South Road, Jishou 416000, Hunan, China. wangle510@foxmail.com.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. imzhuyue@foxmail.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. wangpeng27085@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. fanqiang1022@foxmail.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fan
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. wuyao@im.ac.cn.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Hunan Provincial Key Laboratory of Plant Resources Conservation and Utilization, College of Biology and Environmental Sciences, Jishou University, No. 120 Ren Min South Road, Jishou 416000, Hunan, China. qzpengjsu@163.com.
authors.forename: Qing-Zhong
authors.initials: QZ
authors.lastname: Peng
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. xiagx@im.ac.cn.
authors.forename: Gui-Xian
authors.initials: GX
authors.lastname: Xia
authors.affiliation: The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. wujiahe@im.ac.cn.
authors.forename: Jia-He
authors.initials: JH
authors.lastname: Wu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D000872:Anthocyanins / Q000096:biosynthesis; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis / Q000096:biosynthesis; D003001:Cloning, Molecular / Q000379:methods*; D032561:Cotton Fiber; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D044945:Proanthocyanidins / Q000096:biosynthesis
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D044945:Proanthocyanidins; D002392:Catechin; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase; C057580:gallocatechol
keywords: anthocyanins; dihydroflavanol-4-reductase (DFR); flavan-3-ols; proanthocyanidins (PAs); upland cotton (Gossypium hirsutum)
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