title: Early Molecular Events in Murine Gastric Epithelial Cells Mediated by Helicobacter pylori CagA.
abstract: BACKGROUND
  Murine models of Helicobacter pylori infection are used to study host-pathogen interactions, but lack of severe gastritis in this model has limited its usefulness in studying pathogenesis. We compared the murine gastric epithelial cell line GSM06 to the human gastric epithelial AGS cell line to determine whether similar events occur when cultured with H. pylori.
  
  
  METHODS
  The lysates of cells infected with H. pylori isolates or an isogenic cagA-deficient mutant were assessed for translocation and phosphorylation of CagA and for activation of stress pathway kinases by immunoblot.
  
  
  RESULTS
  Phosphorylated CagA was detected in both cell lines within 60 minutes. Phospho-ERK 1/2 was present within several minutes and distinctly present in GSM06 cells at 60 minutes. Similar results were obtained for phospho-JNK, although the 54 kDa phosphoprotein signal was dominant in AGS, whereas the lower molecular weight band was dominant in GSM06 cells.
  
  
  CONCLUSIONS
  These results demonstrate that early events in H. pylori pathogenesis occur within mouse epithelial cells similar to human cells and therefore support the use of the mouse model for the study of acute CagA-associated host cell responses. These results also indicate that reduced disease in H. pylori-infected mice may be due to lack of the Cag PAI, or by differences in the mouse response downstream of the initial activation events.
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Aditi
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Department of Microbiology, Rastraguru Surendra Nath College, Barrackpore, India.
authors.forename: Malini
authors.initials: M
authors.lastname: Basu
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD, USA. tblanchard@peds.umaryland.edu.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Blanchard
authors.affiliation: Covance, Inc., Indianapolis, IN, USA.
authors.forename: Subba R
authors.initials: SR
authors.lastname: Chintalacharuvu
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Guang
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Erik P
authors.initials: EP
authors.lastname: Lillehoj
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Czinn
mesh_terms: D000328:Adult; D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology* / Q000502:physiology*; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D015151:Immunoblotting; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D015398:Signal Transduction
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori; D011494:Protein Kinases
keywords: CagA; Helicobacter pylori; translocation; type 4 secretion system
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