title: Sequence-Specific Recognition of MicroRNAs and Other Short Nucleic Acids with Solid-State Nanopores.
abstract: The detection and quantification of short nucleic acid sequences has many potential applications in studying biological processes, monitoring disease initiation and progression, and evaluating environmental systems, but is challenging by nature. We present here an assay based on the solid-state nanopore platform for the identification of specific sequences in solution. We demonstrate that hybridization of a target nucleic acid with a synthetic probe molecule enables discrimination between duplex and single-stranded molecules with high efficacy. Our approach requires limited preparation of samples and yields an unambiguous translocation event rate enhancement that can be used to determine the presence and abundance of a single sequence within a background of nontarget oligonucleotides.
authors.affiliation: Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest University School of Medicine , Winston-Salem, North Carolina 27101, United States.
authors.forename: Osama K
authors.initials: OK
authors.lastname: Zahid
authors.affiliation: Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest University School of Medicine , Winston-Salem, North Carolina 27101, United States.
authors.forename: Fanny
authors.initials: F
authors.lastname: Wang
authors.affiliation: Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro , Greensboro, North Carolina 27401, United States.
authors.forename: Jan A
authors.initials: JA
authors.lastname: Ruzicka
authors.affiliation: Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro , Greensboro, North Carolina 27401, United States.
authors.forename: Ethan W
authors.initials: EW
authors.lastname: Taylor
authors.affiliation: Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest University School of Medicine , Winston-Salem, North Carolina 27101, United States.|Comprehensive Cancer Center, Wake Forest University School of Medicine , Winston-Salem, North Carolina 27101, United States.
authors.forename: Adam R
authors.initials: AR
authors.lastname: Hall
mesh_terms: D004247:DNA / Q000032:analysis / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008958:Models, Molecular; D058608:Nanopores / Q000648:ultrastructure; D009369:Neoplasms / Q000235:genetics; D009693:Nucleic Acid Hybridization
chemical_list: D035683:MicroRNAs; D004247:DNA
keywords: Nanopore; cancer; detection; microRNA; sequence
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