title: A fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection.
abstract: A fluorescent immunochromatographic strip test (ICST) based on the use of Quantum Dots (QD) was developed and applied to detect fumonisins in maize samples. A limit of detection for fumonisin B1 of 2.8 µg L(-1) was achieved, with an analytical working range of 3-350 µg L(-1), corresponding to 30-3500 µg kg(-1) in maize flour samples, according with the extraction procedure. The time required to perform the analysis was 22 min, including sample preparation. Recovery values in the range from 91.4% to 105.4% with coefficients of variation not exceeding 5% were obtained for fortified and naturally contaminated maize flour samples. To evaluate the possible improvements due to the use of QD for ICST technology, we performed a direct comparison of the proposed QD-ICST to a gold nanoparticles- and a chemiluminescent-ICST previously developed for fumonisins detection, in which the same immunoreagents were employed.
authors.affiliation: Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Di Nardo
authors.affiliation: Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy. Electronic address: laura.anfossi@unito.it.
authors.forename: L
authors.initials: L
authors.lastname: Anfossi
authors.affiliation: Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Giovannoli
authors.affiliation: Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Passini
authors.affiliation: Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia; Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: V V
authors.initials: VV
authors.lastname: Goftman
authors.affiliation: Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia.
authors.forename: I Y
authors.initials: IY
authors.lastname: Goryacheva
authors.affiliation: Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Baggiani
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003101:Collodion / Q000737:chemistry; D005433:Flour / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D008567:Membranes, Artificial; D045663:Quantum Dots / Q000737:chemistry*; D011934:Reagent Strips / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D037341:Fumonisins; D008567:Membranes, Artificial; D011934:Reagent Strips; C056933:fumonisin B1; D003101:Collodion
keywords: Fumonisin B1; Immunoassay; Immunochromatography; Maize; Quantum Dot