title: Detection of Helicobacter pylori in Nasal Polyps.
abstract: To detect the presence of Helicobacter pylori in nasal polyps. A case-control study was conducted enrolling 35 patients with nasal polyps (cases) and patients undergoing septoplasty (controls). Fresh tissue samples were used for urea broth test and imprint cytology, while formalin fixed tissue sections were used for morphology, special stains and immunohistochemistry for H. pylori. Fresh stool samples from both groups were tested to correlate the gastrointestinal status. H. pylori was detected in 40.0 % (14/35) of cases and 8.5 % of controls (3/35) (p = 0.004) by immunohistochemistry. Amongst cases, eight were positive with urea broth test, six with imprint cytology (Giemsa stain), three with H & E, and nine with modified McMullen's stain. Hyperplasia of the lining epithelium and lymphoid aggregates were significantly noticed in nasal polyps positive for H. pylori. Stool antigen test was positive in subjects who were positive for H. pylori in the nasal mucosa. There appears to be an association between H. pylori and nasal polyps. Immunohistochemistry is more sensitive and specific method to detect H. pylori. H. pylori induced inflammatory tissue reaction pattern indicates a possible causal association. Further studies are needed to prove the causal relationship between H. pylori and nasal polyps.
authors.affiliation: Department of Pathology, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, 110095, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Bansal
authors.affiliation: Department of Pathology, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, 110095, India. sonald76@gmail.com.
authors.forename: Sonal
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Pathology, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, 110095, India.
authors.forename: Sarla
authors.initials: S
authors.lastname: Agarwal
authors.affiliation: Department of Microbiology, University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, India.
authors.forename: Rumpa
authors.initials: R
authors.lastname: Saha
authors.affiliation: Department of Otorhinolaryngology, University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, India.
authors.forename: Neelima
authors.initials: N
authors.lastname: Gupta
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009297:Nasal Mucosa / Q000382:microbiology; D009298:Nasal Polyps / Q000150:complications* / Q000382:microbiology*
keywords: Chronic rhinosinusitis; Giemsa stain; Helicobacter pylori; Immunohistochemistry; Imprint cytology; Modified McMullen’s stain; Nasal polyps; Stool antigen test; Urea broth test
references.citation: Med Glas (Zenica). 2012 Aug;9(2):281-6
references.pmid: 22926364
references.citation: Helicobacter. 2010 Sep;15 Suppl 1:60-8
references.pmid: 21054655
references.citation: Pol Merkur Lekarski. 2005 Sep;19(111):309-11
references.pmid: 16358853
references.citation: J Coll Physicians Surg Pak. 2010 Nov;20(11):773-5
references.pmid: 21078257
references.citation: J Laryngol Otol. 2003 Jan;117(1):1-9
references.pmid: 12590849
references.citation: Lancet. 1983 Jun 4;1(8336):1273-5
references.pmid: 6134060
references.citation: Laryngoscope. 2004 Nov;114(11):1941-4
references.pmid: 15510018
references.citation: Clin Microbiol Rev. 2006 Jul;19(3):449-90
references.pmid: 16847081
references.citation: Acta Cytol. 2000 Mar-Apr;44(2):124-7
references.pmid: 10740594
references.citation: Laryngoscope. 2003 Apr;113(4):679-82
references.pmid: 12671428
references.citation: Indian J Otolaryngol Head Neck Surg. 2005 Jul;57(3):199-201
references.pmid: 23120171
references.citation: Jundishapur J Microbiol. 2015 Mar 21;8(3):e20783
references.pmid: 25973159
references.citation: Laryngoscope. 2005 Jun;115(6):946-57
references.pmid: 15933499
references.citation: Auris Nasus Larynx. 2009 Aug;36(4):427-30
references.pmid: 19010623
references.citation: Am J Rhinol Allergy. 2015 May-Jun;29(3):e70-4
references.pmid: 25975241
references.citation: Laryngoscope. 2003 Sep;113(9):1557-63
references.pmid: 12972933
references.citation: Laryngoscope. 2001 Dec;111(12):2183-6
references.pmid: 11802022
references.citation: Otolaryngol Head Neck Surg. 2006 Jan;134(1):67-72
references.pmid: 16399183
references.citation: J Otolaryngol Head Neck Surg. 2008 Apr;37(2):192-5
references.pmid: 19128611
references.citation: J Laryngol Otol. 2003 Feb;117(2):118-21
references.pmid: 12625884
references.citation: BMC Res Notes. 2010 Jul 09;3:186
references.pmid: 20618972
references.citation: Arch Med Res. 2011 Nov;42(8):686-9
references.pmid: 22222490
references.citation: Indian J Pathol Microbiol. 2008 Jan-Mar;51(1):26-9
references.pmid: 18417845
references.citation: Gastroenterol Clin North Am. 2000 Dec;29(4):879-84
references.pmid: 11190072
references.citation: Acta Otorhinolaryngol Ital. 2011 Dec;31(6):372-7
references.pmid: 22323848
references.citation: Postgrad Med J. 1993 Apr;69(810):291-5
references.pmid: 8321793
references.citation: J Clin Pathol. 2000 Oct;53(10):756-9
references.pmid: 11064668
references.citation: Aliment Pharmacol Ther. 2001 Dec;15(12):1913-9
references.pmid: 11736722
references.citation: Eur Arch Otorhinolaryngol. 2012 May;269(5):1457-61
references.pmid: 22116383
references.citation: Otolaryngol Head Neck Surg. 2009 Jul;141(1):131-5
references.pmid: 19559972
references.citation: Otolaryngol Head Neck Surg. 2007 Mar;136(3):390-5
references.pmid: 17321865
references.citation: IARC Monogr Eval Carcinog Risks Hum. 1994;61:1-241
references.pmid: 7715068
references.citation: Acta Otolaryngol. 2002 Mar;122(2):179-82
references.pmid: 11936910
references.citation: World J Gastroenterol. 2014 May 21;20(19):5639-53
references.pmid: 24914323
references.citation: Am J Rhinol Allergy. 2013 Nov-Dec;27(6):473-8
references.pmid: 24274222