title: Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses.
abstract: A considerable amount of work has been undertaken to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50 % of infected larvae (LC50 or LD50), median survival of larvae infected (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and the corresponding data analyses for assessment of the insecticidal activity of baculovirus insecticides.
authors.affiliation: USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, USA. hpopham@agbitech.com.|AgBiTech, 1601 S. Providence Rd., Columbia, MO, 65211, USA. hpopham@agbitech.com.
authors.forename: Holly J R
authors.initials: HJ
authors.lastname: Popham
authors.affiliation: Department of Statistics, University of Missouri, Columbia, MO, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Ellersieck
authors.affiliation: Dow AgroSciences Discovery Research, Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Huarong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Entomology, Iowa State University, Ames, IA, USA.
authors.forename: Bryony C
authors.initials: BC
authors.lastname: Bonning
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D018929:Cell Culture Techniques; D004274:DNA, Recombinant / Q000235:genetics*; D007814:Larva / Q000821:virology; D007928:Lethal Dose 50; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D010572:Pest Control, Biological / Q000379:methods*; D018570:Risk Assessment
chemical_list: D004274:DNA, Recombinant
keywords: Diet plug bioassay; Droplet feeding; Feeding damage; Host range; Insecticidal efficacy; LC50; LD50; Microapplicator; Recombinant baculovirus; ST50