title: Isolation, Identification, and Evaluation of Novel Probiotic Strains Isolated from Feces of Breast-Fed Infants.
abstract: OBJECTIVE
  To isolate, identify, and evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from the feces of breast-fed infants.
  
  
  METHODS
  The probiotic tests included investigation of hemolysis activity, survival in simulated gastrointestinal tract conditions (acid and bile salt tolerance), susceptibility to antibiotics, and ability to inhibit selected bacterial pathogens (Escherichia coli O157:H7, Vibrio cholerae and Salmonella enterica subsp enterica serovar Typhimurium). The bacterial species identification was performed by both carbohydrate utilization and partial 16S ribosomal RNA sequencing.
  
  
  RESULTS
  Five of fifty LAB isolates (UBU-03, UBU-06, UBU-09, UBU-34, and UBU-37) showed good probiotic properties. These five isolates showed non-hemolysis type (gamma-hemolysis), susceptibility to all antibiotics tested except for vancomycin, ability to survive in the simulated gastrointestinal conditions of both acid and bile salt solution, and ability to inhibit growth of E. coli O157: H7 and V. cholerae. Bacterial species identification revealed that all five isolates were firmly identified as Lactobacillus rhamnosus species.
  
  
  CONCLUSIONS
  The L. rhamnosus strains that were isolated and characterized in this study could be considered as probiotic strains, and then used for further probiotic characterization in human cell cultures or animal models.
authors.forename: Marutpong
authors.initials: M
authors.lastname: Panya
authors.forename: Viraphong
authors.initials: V
authors.lastname: Lulitanond
authors.forename: Pongsak
authors.initials: P
authors.lastname: Rattanachaikunsopon
authors.forename: Thanyakarn
authors.initials: T
authors.lastname: Srivoramas
authors.forename: Tarinee
authors.initials: T
authors.lastname: Chaiwong
mesh_terms: D001942:Breast Feeding; D004269:DNA, Bacterial / Q000032:analysis; D019453:Escherichia coli O157 / Q000187:drug effects; D005243:Feces / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D007231:Infant, Newborn; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D008297:Male; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000302:isolation & purification / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects; D014734:Vibrio cholerae / Q000187:drug effects
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S