title: Small-Scale Production of Recombinant Proteins Using the Baculovirus Expression Vector System.
abstract: Numerous technological improvements, including progress in vector design, simplification of virus isolation techniques, and advancements in molecular biology and cell culture technologies, have greatly facilitated the use of the baculovirus-insect cell system for routine production of recombinant proteins. This chapter outlines the basic techniques for small-scale protein production using the Baculovirus Expression Vector System (BEVS), including protocols for titer estimation in 96-well plates, expression optimization in 24-well plates, and recombinant protein expression from adherent and suspension cultures in six-well plates and in 50 mL insect cell cultures.
authors.affiliation: Synthetic Biology, Life Science Solution, ThermoFisher Scientific, 5781 Van Allen Way, Carlsbad, CA, 92008, USA. jianping.yang@thermofisher.com.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Yang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002021:Buffers; D018929:Cell Culture Techniques; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology / Q000821:virology
chemical_list: D002021:Buffers; D003902:Detergents; D011994:Recombinant Proteins
keywords: Baculovirus expression vector system (BEVS); End-point dilution assay; Recombinant protein expression