title: [Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].
abstract: BACKGROUND
  Extracellular vesicles (EVs) are released to the bloodstream by certain cell types due to transport, activation and cell death processes. Blood count of EVs from platelet and endothelial origin has been proved to be a cardiovascular risk biomarker. Thus, EVs proteome might reflect the underlying cellular processes in hypertensive patients with albuminuria.
  
  
  METHODS
  Protein content of circulating EVs was analyzed by liquid chromatography coupled to mass spectrometry. EVs were isolated by an ultracentrifugation protocol optimized in order to avoid contamination by blood plasma proteins. Purity of the isolated fraction was verified by electronic and confocal microscopy, and by flow cytometry.
  
  
  RESULTS
  We hereby show a method to isolate circulating EVs from hypertensive patients with/without albuminuria with high yield and purity. Besides, we provide a reference proteome of the EVs of these patients, composed of 2,463 proteins, and prove that the proteins carried by these vesicles are associated with crucial processes involved in the inherent cardiovascular risk.
  
  
  CONCLUSIONS
  The proteome of circulating EVs is an interesting source of indicators in the evaluation of cardiovascular risk in hypertensive patients with renin-angiotensin system blockage.
authors.affiliation: Laboratorio de Fisiopatología Vascular, Hospital Nacional de Parapléjicos (HNP), Servicio de Salud de Castilla la Mancha (SESCAM), Toledo, España. Electronic address: ferdela@sescam.jccm.es.
authors.forename: F
authors.initials: F
authors.lastname: de la Cuesta
authors.affiliation: Laboratorio de Fisiopatología Vascular, Hospital Nacional de Parapléjicos (HNP), Servicio de Salud de Castilla la Mancha (SESCAM), Toledo, España.
authors.forename: M
authors.initials: M
authors.lastname: Baldan-Martin
authors.affiliation: Laboratorio de Fisiopatología Vascular, Hospital Nacional de Parapléjicos (HNP), Servicio de Salud de Castilla la Mancha (SESCAM), Toledo, España.
authors.forename: L
authors.initials: L
authors.lastname: Mourino-Alvarez
authors.affiliation: Laboratorio de Fisiopatología Vascular, Hospital Nacional de Parapléjicos (HNP), Servicio de Salud de Castilla la Mancha (SESCAM), Toledo, España.
authors.forename: T
authors.initials: T
authors.lastname: Sastre-Oliva
authors.affiliation: Departamento de Inmunología, IIS-Fundación Jiménez Díaz, Madrid, España.
authors.forename: G
authors.initials: G
authors.lastname: Alvarez-Llamas
authors.affiliation: Departamento de Inmunología, IIS-Fundación Jiménez Díaz, Madrid, España.
authors.forename: L
authors.initials: L
authors.lastname: Gonzalez-Calero
authors.affiliation: Departamento de Riesgo Cardiovascular e Hipertensión, IIS-Hospital 12 de Octubre, Madrid, España.
authors.forename: G
authors.initials: G
authors.lastname: Ruiz-Hurtado
authors.affiliation: Unidad de Hipertensión, Hospital 12 de Octubre, Madrid, España.
authors.forename: J
authors.initials: J
authors.lastname: Segura
authors.affiliation: Departamento de Inmunología, IIS-Fundación Jiménez Díaz, Madrid, España.
authors.forename: F
authors.initials: F
authors.lastname: Vivanco
authors.affiliation: Departamento de Riesgo Cardiovascular e Hipertensión, IIS-Hospital 12 de Octubre, Madrid, España.
authors.forename: L M
authors.initials: LM
authors.lastname: Ruilope
authors.affiliation: Laboratorio de Fisiopatología Vascular, Hospital Nacional de Parapléjicos (HNP), Servicio de Salud de Castilla la Mancha (SESCAM), Toledo, España.
authors.forename: M G
authors.initials: MG
authors.lastname: Barderas
mesh_terms: D001792:Blood Platelets; D001798:Blood Proteins; D002318:Cardiovascular Diseases / Q000453:epidemiology*; D002853:Chromatography, Liquid; D000067128:Extracellular Vesicles; D005434:Flow Cytometry; D006801:Humans; D020543:Proteome; D012084:Renin-Angiotensin System; D012307:Risk Factors; D022142:Secretory Vesicles; D022161:Transport Vesicles
chemical_list: D001798:Blood Proteins; D020543:Proteome
keywords: Albuminuria; Bloqueo sistema renina-angiotensina; Extracellular vesicles; Proteomics; Proteómica; Renin-angiotensin system blockage; Vesículas extracelulares