title: Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA.
abstract: Previously, we demonstrated specific accumulation into bacteria of a 12-mer phosphorodiamidate morpholino (MORF) oligomer complementary to a ribosomal RNA (rRNA) segment found in all bacteria using the universal probe called Eub338 (Eub). Here, two MORF oligomers Eco and Kpn with sequences specific to the rRNA of Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) were investigated along with Eub and control (nonEub).
  
  
  
  To determine bacterial rRNA binding, oligomers were tagged with Alexa Fluor 633 (AF633) for fluorescence in situ hybridization (FISH) and fluorescence microscopy, and radiolabeled with technetium-99m (Tc-99m) for biodistribution and SPECT imaging in infected mice.
  
  
  
  By both FISH and fluorescence microscopy, Eub showed a positive signal in both E. coli and K. pneumoniae as expected, and Kpn showed significantly higher accumulation in K. pneumoniae with near background in E. coli (p < 0.01). Conversely, Eco was positive in both E. coli and K. pneumoniae, hence nonspecific. As determined by biodistribution, the accumulation of [(99m)Tc]Kpn was higher in the thigh infected with live K. pneumoniae than with live E. coli (p = 0.05), and significantly higher than with heat-killed K. pneumoniae (p = 0.02) in the target thigh. By SPECT imaging, the accumulation of [(99m)Tc]Kpn was obviously higher in its specific target of K. pneumoniae compared to an E. coli infected thigh.
  
  
  
  Kpn complementary to the rRNA of K. pneumoniae, labeled with Tc-99m or AF633, demonstrated specific binding to fixed and live K. pneumoniae in culture and in infected mice such that Tc-99m-labeled Kpn as the MORF oligomer may be useful for K. pneumoniae infection detection through imaging.
authors.affiliation: Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
authors.forename: Guozheng
authors.initials: G
authors.lastname: Liu
authors.affiliation: Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
authors.forename: Shuping
authors.initials: S
authors.lastname: Dou
authors.affiliation: Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: ImmunoGen Inc, Waltham, MA, 02451, USA.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, , Shanghai, 201800, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA. mary.rusckowski@umassmed.edu.
authors.forename: Mary
authors.initials: M
authors.lastname: Rusckowski
mesh_terms: D000818:Animals; D017404:In Situ Hybridization, Fluorescence; D007710:Klebsiella Infections / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000473:pathology; D007711:Klebsiella pneumoniae / Q000302:isolation & purification*; D051379:Mice; D008856:Microscopy, Fluorescence; D060172:Morpholinos / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000378:metabolism; D013667:Technetium / Q000378:metabolism; D014018:Tissue Distribution / Q000187:drug effects; D015899:Tomography, Emission-Computed, Single-Photon; D014057:Tomography, X-Ray Computed
chemical_list: D060172:Morpholinos; D016376:Oligonucleotides, Antisense; D012335:RNA, Ribosomal; D019275:Radiopharmaceuticals; D013667:Technetium
keywords: Antisense MORF; Bacterial infection imaging; Bacterial ribosomal RNA
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