title: Overexpression of MicroRNA-221 is associated with poor prognosis in non-small cell lung cancer patients.
abstract: The abnormal expression of microRNA-221 was detected in several cancers and some studies had indicated that microRNA-221 was associated with cancer prognosis. This study was aimed to evaluate the prognostic significance of microRNA-221 in non-small cell lung cancer (NSCLC). Quantitative real-time polymerase chain reaction (qRT-PCR) was used for detecting the relative expression levels of microRNA-221 in the pathological tissues and corresponding normal tissues of 104 NSCLC patients. The relationship between the expression levels and the clinical features was estimated by Chi-square method and the overall survival of patients at different expression levels was demonstrated by Kaplan-Meier method. Cox regression analysis was used to evaluate the prognostic significance of microRNA-221. The relative expression levels of microRNA-221 in the pathological tissues were remarkably higher than that in the corresponding normal tissues (1.71 vs 1.07, P = 0.000). The expression level was associated with lymph node metastasis (P = 0.001). The results of Kaplan-Meier method indicated that patients with high expression level of microRNA-221 had shorter overall survival time than those with low expression level (36.8 vs 45.2 months, P = 0.001). Moreover, Cox regression analysis suggested that microRNA-221 was a useful independent biomarker for NSCLC prognosis (HR = 1.873, 95 % CI = 1.267-2.768, P = 0.002). The aberrant expression of microRNA-221 is associated with NSCLC progression and it might be a potential biomarker for NSCLC prognosis.
authors.affiliation: Medical College, Nankai University, Tianjin, 300071, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Zhefeng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Medical College, Nankai University, Tianjin, 300071, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing, 100853, China. jiaosc@vip.sina.com.
authors.forename: Shunchang
authors.initials: S
authors.lastname: Jiao
mesh_terms: D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012334:RNA, Neoplasm; C525709:MIR221, human
keywords: MicroRNA-221; Non-small cell lung cancer; Prognosis
references.citation: Oncotarget. 2015 Feb 28;6(6):3709-21
references.pmid: 25686829
references.citation: Expert Rev Mol Diagn. 2010 May;10(4):435-44
references.pmid: 20465498
references.citation: Oncol Rep. 2015 Mar;33(3):1221-6
references.pmid: 25586265
references.citation: Front Biosci (Landmark Ed). 2015 Jun 01;20:1092-103
references.pmid: 25961547
references.citation: Oncotarget. 2014 Oct 15;5(19):9410-24
references.pmid: 25238260
references.citation: Oncol Rep. 2015 Jun;33(6):2853-62
references.pmid: 25963391
references.citation: PLoS One. 2011;6(11):e26646
references.pmid: 22114662
references.citation: Tumour Biol. 2015 Jun;36(6):4357-65
references.pmid: 25874488
references.citation: Int J Clin Exp Med. 2015 Feb 15;8(2):2024-30
references.pmid: 25932132
references.citation: Mayo Clin Proc. 2008 Mar;83(3):355-67
references.pmid: 18316005
references.citation: Ann Transl Med. 2015 Apr;3(5):68
references.pmid: 25992367
references.citation: Oncotarget. 2015 Jun 30;6(18):16043-58
references.pmid: 25965824
references.citation: Lab Med. 2015 Spring;46(2):118-22
references.pmid: 25918190
references.citation: J Natl Compr Canc Netw. 2015 May;13(5):515-24
references.pmid: 25964637
references.citation: World J Gastroenterol. 2014 Jun 28;20(24):7894-913
references.pmid: 24976726
references.citation: FEBS Lett. 2014 Jan 3;588(1):99-104
references.pmid: 24269686
references.citation: Cancer Lett. 2015 Jul 1;362(2):208-17
references.pmid: 25843294
references.citation: Ann Oncol. 2011 Feb;22(2):376-82
references.pmid: 20699278
references.citation: Acta Biochim Biophys Sin (Shanghai). 2015 Jun;47(6):431-40
references.pmid: 25935837
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438
references.citation: Front Physiol. 2015 Feb 20;6:50
references.pmid: 25750628
references.citation: Mayo Clin Proc. 2008 May;83(5):584-94
references.pmid: 18452692
references.citation: Am J Cancer Res. 2015 Jan 15;5(2):802-11
references.pmid: 25973317
references.citation: Int J Oncol. 2015 Mar;46(3):927-32
references.pmid: 25571912
references.citation: Mini Rev Med Chem. 2015;15(6):467-74
references.pmid: 25807941
references.citation: Tumour Biol. 2015 Aug;36(8):6497-505
references.pmid: 25833694
references.citation: Cancer Sci. 2015 Jul;106(7):819-24
references.pmid: 25940592
references.citation: CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90
references.pmid: 21296855
references.citation: Nat Rev Cancer. 2009 Apr;9(4):293-302
references.pmid: 19262572
references.citation: PLoS One. 2015 May 11;10(5):e0124428
references.pmid: 25961751
references.citation: Exp Mol Med. 2015 May 08;47:e162
references.pmid: 25952770
references.citation: Int J Clin Exp Pathol. 2015 Feb 01;8(2):1967-72
references.pmid: 25973090
references.citation: Cancer Med. 2015 Apr;4(4):551-64
references.pmid: 25641933