title: Serum Glycans as Risk Markers for Non-Small Cell Lung Cancer.
abstract: Previous studies have suggested occurrence of altered serum glycan profiles in patients with lung cancer. Here, we aimed to determine the predictive value of serum glycans to distinguish non-small cell lung cancer (NSCLC) cases from controls in prediagnostic samples using a previously validated predictive protein marker pro-SFTPB, as anchor. Blinded prediagnostic serum samples were obtained from the Carotene and Retinol Efficacy Trial (CARET), and included a discovery set of 100 NSCLC cases and 199 healthy controls. A second test set consisted of 108 cases and 216 controls. Cases and controls were matched for age at baseline (5-year groups), sex, smoking status (current vs. former), study enrollment cohort, and date of blood draw. Serum glycan profiles were determined by mass spectrometry. Twelve glycan variables were identified to have significant discriminatory power between cases and controls in the discovery set (AUC > 0.6). Of these, four were confirmed in the independent validation set. A combination marker yielded AUCs of 0.74 and 0.64 in the discovery and test set, respectively. Four glycan variables exhibited significant incremental value when combined with pro-SFTPB compared with pro-SFTPB alone with AUCs of 0.73, 0.72, 0.72, and 0.72 in the test set, indicating that serum glycan signatures have relevance to risk assessment for NSCLC.
authors.affiliation: Department of Chemistry, University of California Davis, Davis, California. Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, Texas. lrruhaak@gmail.com.
authors.forename: L Renee
authors.initials: LR
authors.lastname: Ruhaak
authors.affiliation: Department of Chemistry, University of California Davis, Davis, California. Division of Hematology and Oncology, University of California Davis Comprehensive Cancer Center, Sacramento, California.
authors.forename: Carol
authors.initials: C
authors.lastname: Stroble
authors.affiliation: Department of Biostatistics, University of Texas MD Anderson Cancer Center, Houston, Texas.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Dai
authors.affiliation: Division of Public Health Sciences, Fred Hutchison Cancer Research Center, Seattle, Washington.
authors.forename: Matt
authors.initials: M
authors.lastname: Barnett
authors.affiliation: Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, Texas.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Taguchi
authors.affiliation: Division of Public Health Sciences, Fred Hutchison Cancer Research Center, Seattle, Washington.
authors.forename: Gary E
authors.initials: GE
authors.lastname: Goodman
authors.affiliation: Division of Hematology and Oncology, University of California Davis Comprehensive Cancer Center, Sacramento, California.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Miyamoto
authors.affiliation: Division of Hematology and Oncology, University of California Davis Comprehensive Cancer Center, Sacramento, California.
authors.forename: David
authors.initials: D
authors.lastname: Gandara
authors.affiliation: Department of Biostatistics, University of Texas MD Anderson Cancer Center, Houston, Texas.
authors.forename: Ziding
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Department of Chemistry, University of California Davis, Davis, California.
authors.forename: Carlito B
authors.initials: CB
authors.lastname: Lebrilla
authors.affiliation: Department of Clinical Cancer Prevention - Research, Clinical Cancer Prevention, University of Texas MD Anderson Cancer Center, Houston, Texas.
authors.forename: Samir
authors.initials: S
authors.lastname: Hanash
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011134:Polysaccharides / Q000097:blood*; D011237:Predictive Value of Tests; D011498:Protein Precursors / Q000097:blood*; D037661:Pulmonary Surfactant-Associated Proteins / Q000097:blood*; D016032:Randomized Controlled Trials as Topic; D018570:Risk Assessment; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D011134:Polysaccharides; D011498:Protein Precursors; D037661:Pulmonary Surfactant-Associated Proteins; C586477:SFTPB protein, human
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