title: Pore formation mechanism of porous poly(DL-lactic acid) matrix membrane.
abstract: Porous PLA structure has been widely used in cell transplantation, drug carrier and wound dressing. The porous structure can be controlled depending on the choice of the polymer, solvent, nonsolvent and preparation parameters. In this study, the porous PLA matrix membranes were prepared by adding PEG 400 in PLA solution using dichloromethane (DCM) as solvent prior to casting. The influence of other liquids as co-solvent on pore formation and the structural change during membrane formation were evaluated. The co-solvents affected both porous topography and mechanical properties of PLA membrane. The porous matrix were produced when the non-solvent of PLA was used as co-solvent. Cryo-SEM micrographs revealed that PEG 400 still remained in the PLA porous matrix membrane. From the tracking of the structural change during film formation, the PLA-PEG solution changed into porous structure by liquid liquid phase separation and solidification processes, respectively. Thermogravimetric analysis revealed that PLA-PEG in DCM solution exhibited the two-step of weight loss, the first step occurred from DCM evaporation and the second step occurred from the degradation of PLA-PEG matrix. The liquid-liquid phase separation and solidification started when the amount of DCM was higher than PEG 400 for 2.67 folds and DCM amount was equal to that of PEG 400, respectively. These results could clarify the pore formation mechanism of porous PLA membrane and will be useful for the further investigation and application.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand. Electronic address: tphaechamud011@yahoo.com.
authors.forename: Thawatchai
authors.initials: T
authors.lastname: Phaechamud
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand. Electronic address: sasi_toey@hotmail.com.
authors.forename: Sasiprapa
authors.initials: S
authors.lastname: Chitrattha
mesh_terms: D020285:Cryoelectron Microscopy; D019344:Lactic Acid / Q000737:chemistry*; D008752:Methylene Chloride / Q000737:chemistry; D044367:Phase Transition; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013500:Surface Tension; D013718:Tensile Strength; D013818:Thermogravimetry
chemical_list: D011091:Polyesters; D011108:Polymers; D012997:Solvents; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D008752:Methylene Chloride; C000595213:polyethylene glycol 400
keywords: Mechanism; Poly(DL-lactic acid); Pore formation; Porous membrane