title: Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes.
abstract: Hypercholesterolemia drives the development of cardiovascular disease, the leading cause of mortality in western society. Supplementation with probiotics that interfere with cholesterol metabolism may provide a contribution to disease prevention. Lactobacillus plantarum CUL66 (NCIMB 30280) has been assessed in vitro for its ability to impact cholesterol absorption. L. plantarum CUL66 tested positive for bile salt hydrolase activity and the ability to assimilate cholesterol from culture media. RT-qPCR analysis showed that the bacterium significantly decreased the expression of Niemann-Pick C1-like 1 and ATP-binding cassette transporter-1 in polarised Caco-2 cells after 6 h exposure. Conversely, the expression of ATP-binding cassette sub-family G member (ABCG)-5 and ABCG-8, and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased. Using a radiolabelled assay, we also observed significant reductions in the uptake and basolateral efflux of cholesterol by Caco-2 cells exposed to L. plantarum CUL66. This in vitro study identified L. plantarum CUL66 as a cholesterol lowering bacteria by highlighting its ability to beneficially regulate multiple in vitro events associated with intestinal cholesterol metabolism and provides evidence of efficacy for its inclusion in future in vivo studies.
authors.affiliation: 1 Cultech Limited, Unit 2 Christchurch Road, Baglan Industrial Park, Port Talbot SA12 7BZ, United Kingdom.
authors.forename: D R
authors.initials: DR
authors.lastname: Michael
authors.affiliation: 2 Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, United Kingdom.
authors.forename: J W E
authors.initials: JW
authors.lastname: Moss
authors.affiliation: 1 Cultech Limited, Unit 2 Christchurch Road, Baglan Industrial Park, Port Talbot SA12 7BZ, United Kingdom.
authors.forename: D Lama
authors.initials: DL
authors.lastname: Calvente
authors.affiliation: 1 Cultech Limited, Unit 2 Christchurch Road, Baglan Industrial Park, Port Talbot SA12 7BZ, United Kingdom.
authors.forename: I
authors.initials: I
authors.lastname: Garaiova
authors.affiliation: 1 Cultech Limited, Unit 2 Christchurch Road, Baglan Industrial Park, Port Talbot SA12 7BZ, United Kingdom.
authors.forename: S F
authors.initials: SF
authors.lastname: Plummer
authors.affiliation: 2 Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, United Kingdom.
authors.forename: D P
authors.initials: DP
authors.lastname: Ramji
mesh_terms: D018938:Caco-2 Cells; D002784:Cholesterol / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism* / Q000382:microbiology*; D020869:Gene Expression Profiling; D006706:Homeostasis; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D002784:Cholesterol
keywords: L. plantarum; efflux; enterocyte; hypercholesterolemia; uptake