title: Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells.
abstract: We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.
authors.affiliation: Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary.
authors.forename: Badri L
authors.initials: BL
authors.lastname: Aekbote
authors.affiliation: Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Fekete
authors.affiliation: University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020 Linz, Austria.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Jacak
authors.affiliation: Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary.
authors.forename: Gaszton
authors.initials: G
authors.lastname: Vizsnyiczai
authors.affiliation: Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary.
authors.forename: Pál
authors.initials: P
authors.lastname: Ormos
authors.affiliation: Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary.
authors.forename: Lóránd
authors.initials: L
authors.lastname: Kelemen
keywords: (140.7090) Ultrafast lasers; (160.1435) Biomaterials; (160.5470) Polymers; (180.2520) Fluorescence microscopy; (230.4000) Microstructure fabrication; (230.6120) Spatial light modulators; (310.0310) Thin films; (350.3390) Laser materials processing; (350.4855) Optical tweezers or optical manipulation
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