title: Primers for low-copy nuclear genes in the Melastomataceae.
abstract: OBJECTIVE
  Low-copy nuclear gene primers were developed for phylogenetic studies across the Melastomataceae.
  
  
  RESULTS
  Total genomic libraries from eight species in the Melastomataceae along with one transcriptome were used for marker identification and primer design. Eight exon-primed intron-crossing markers were amplified with success in taxa of nine tribes in the Melastomataceae. The new markers were directly sequenced for eight samples of closely related species of Miconia (Chaenanthera clade) in the tribe Miconieae. The DNA sequences for the eight loci ranged from 660 to 818 aligned base pairs. Compared with four commonly used markers in other studies, the loci developed here had a higher number of variable sites than plastid spacers (7-16 vs. 26-45) and comparable variation to the ribosomal spacers (28-39).
  
  
  CONCLUSIONS
  The novel primer pairs should be useful for a broad range of studies of systematics and evolution in the diverse Melastomataceae.
authors.affiliation: Institute of Systematic Botany, The New York Botanical Garden, Bronx, New York 10458 USA.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Reginato
authors.affiliation: Institute of Systematic Botany, The New York Botanical Garden, Bronx, New York 10458 USA.
authors.forename: Fabián A
authors.initials: FA
authors.lastname: Michelangeli
keywords: Melastomataceae; Miconieae; phylogeny; single copy; systematics
references.citation: Mol Biol Evol. 2013 Apr;30(4):772-80
references.pmid: 23329690
references.citation: Genetics. 2006 Nov;174(3):1407-20
references.pmid: 16951058
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Mol Phylogenet Evol. 2015 Jan;82 Pt A:289-313
references.pmid: 25451807
references.citation: Am J Bot. 2001 Mar;88(3):486-98
references.pmid: 11250827