title: Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb(2+) using molecular beacon and DNAzyme based amplification strategy.
abstract: An enzyme-free and label-free colorimetric Pb(2+) sensor based on DNAzyme and molecular beacon (MB) has been developed and demonstrated by recycle using enzyme strand for signal amplification. The substrate strand DNA (S-DNA) of DNAzyme could be converted into MB structure with base pairs of stem part at the both ends. The MB could hybridize with enzyme strand DNA (E-DNA) to form DNAzyme, and be activated and cleaved in the presence of Pb(2+). The cleaved MB is much less stable, releasing from the DNAzyme as two product pieces. The product pieces of MB are flexible and could bind to unmodified AuNPs to effectively stabilize them against salt-induced aggregation. Then, the E-DNA is liberated to catalyze the next reaction and amplify the response signal. By taking advantage of repeated using of E-DNA, our proposed method exhibited high sensitive for Pb(2+) detection in a linear range from 0.05 to 5 nM with detection limit of 20 pM by UV-vis spectrometer. Moreover, this method was also used for determination of Pb(2+) in river water samples with satisfying results. Importantly, this strategy could reach high sensitivity without any modification and complex enzymatic or hairpins based amplification procedures.
authors.affiliation: Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yun
authors.affiliation: Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.
authors.forename: Dingzhou
authors.initials: D
authors.lastname: Cai
authors.affiliation: Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.
authors.forename: JiaoLai
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.
authors.forename: Pengxiang
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, No. 266 Fangzheng Avenue, Shuitu Hi-Tech Park, Shuitu Town, Beibei District, Chongqing 400714, PR China. Electronic address: huangyu@cigit.ac.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China. Electronic address: yunwenyunwen@qq.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Sang
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D004247:DNA / Q000737:chemistry; D021881:DNA, Catalytic / Q000737:chemistry; D007854:Lead / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D021881:DNA, Catalytic; D007854:Lead; D004247:DNA
keywords: DNAzyme; Gold nanoparticles; Molecular beacon; Pb(2+) detection