title: Effect of oleic acid on store-operated calcium entry in immune-competent cells.
abstract: OBJECTIVE
  To study the mechanism by which oleic acid (OA) (C18:1) exerts its beneficial effects on immune-competent cells. Since store-operated Ca2+entry (SOCE) is a Ca2+influx pathway involved in the control of multiple physiological processes including cell proliferation, we studied the effect of OA in Ca2+signals of Jurkat T cells and THP-1 monocytes, paying particular attention to SOCE.
  
  
  METHODS
  Changes in [Ca2+]iwere measured using the Fura-2 fluorescence dye. Mn2+uptake was monitored as a rate of quenching of Fura-2 fluorescence measured at the Ca2+-insensitive wavelengths. Thapsigargin was used to induce SOCE in Fura-2-loaded cells.
  
  
  RESULTS
  We showed a clear dose-dependent SOCE-inhibitory effect of OA in both cell lines. Such an inhibitory effect was PKC independent and totally restored by albumin, suggesting that OA exerts its effect somewhere in the membrane. We also demonstrated that OA induces increases in [Ca2+]ipartly mediated by an extracellular Ca2+influx through econazole-insensitive channels. Finally, we compared the effect of OA with stearic acid (C18:0), assuming the emerged evidence concerning the link between saturated fats and inflammation disorders. Stearic acid failed to inhibit SOCE, independently on the concentration tested, thus intensifying the physiological relevance of our findings.
  
  
  CONCLUSIONS
  We suggest a physiological pathway for the beneficial effects of OA in inflammation.
authors.affiliation: Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain. ccarrillo@ubu.es.
authors.forename: Celia
authors.initials: C
authors.lastname: Carrillo
authors.affiliation: Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Giraldo
authors.affiliation: Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: M Mar
authors.initials: MM
authors.lastname: Cavia
authors.affiliation: Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: Sara R
authors.initials: SR
authors.lastname: Alonso-Torre
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004464:Econazole / Q000494:pharmacology; D016257:Fura-2 / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D008345:Manganese / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology*; D019284:Thapsigargin / Q000494:pharmacology*
chemical_list: D019301:Oleic Acid; D008345:Manganese; D019284:Thapsigargin; D004464:Econazole; D002118:Calcium; D016257:Fura-2
keywords: Ca2+ signals; Immune-competent cells; Oleic acid; SOCE
references.citation: Mini Rev Med Chem. 2013 Feb;13(2):201-10
references.pmid: 23278117
references.citation: Biochim Biophys Acta. 1997 Sep 4;1328(2):207-13
references.pmid: 9315617
references.citation: Nutrients. 2014 Sep 04;6(9):3474-500
references.pmid: 25192027
references.citation: Biochim Biophys Acta. 2015 Apr;1851(4):469-84
references.pmid: 25149823
references.citation: Eur J Nutr. 2012 Sep;51(6):677-84
references.pmid: 21956128
references.citation: Biochem Biophys Res Commun. 1989 Jul 14;162(1):24-9
references.pmid: 2546547
references.citation: Vet Immunol Immunopathol. 2013 Feb 15;151(3-4):275-84
references.pmid: 23267746
references.citation: Biochim Biophys Acta. 2012 Apr;1821(4):618-26
references.pmid: 22306362
references.citation: Biochem J. 1991 Jul 1;277 ( Pt 1):73-9
references.pmid: 1906712
references.citation: Inflammation. 2014 Apr;37(2):467-77
references.pmid: 24221358
references.citation: Ann Rheum Dis. 2015 Jan;74(1):303-10
references.pmid: 24285492
references.citation: Biochem J. 1992 Feb 15;282 ( Pt 1):33-9
references.pmid: 1311559
references.citation: Ann Nutr Metab. 2009;55(1-3):123-39
references.pmid: 19752539
references.citation: J Rheumatol Suppl. 2010 May;85:2-10
references.pmid: 20436161
references.citation: Atherosclerosis. 2009 May;204(1):147-55
references.pmid: 18842266
references.citation: Cell Calcium. 1990 Nov-Dec;11(10):641-6
references.pmid: 2095981
references.citation: Mol Pharmacol. 2007 Dec;72(6):1545-56
references.pmid: 17878267
references.citation: Physiol Rev. 2005 Apr;85(2):757-810
references.pmid: 15788710
references.citation: Arterioscler Thromb Vasc Biol. 2012 Jul;32(7):1687-95
references.pmid: 22556332
references.citation: Cell Calcium. 1997 May;21(5):375-85
references.pmid: 9174650
references.citation: Cell Physiol Biochem. 2011;27(3-4):373-80
references.pmid: 21471726
references.citation: J Physiol. 2006 Feb 15;571(Pt 1):57-73
references.pmid: 16339178
references.citation: Proc Natl Acad Sci U S A. 1990 Apr;87(7):2466-70
references.pmid: 2138778
references.citation: Cell Physiol Biochem. 2011;28(2):329-38
references.pmid: 21865741
references.citation: Biochem Biophys Res Commun. 2011 Jun 3;409(2):280-6
references.pmid: 21575602
references.citation: Nutr J. 2014 Jun 17;13:61
references.pmid: 24939238
references.citation: FEBS Lett. 1994 Oct 17;353(2):167-70
references.pmid: 7926044
references.citation: Int J Cancer. 2001 Jun 15;92(6):877-82
references.pmid: 11351310
references.citation: Mol Cell Endocrinol. 1994 Jul;103(1-2):125-32
references.pmid: 7958389
references.citation: J Biol Chem. 1985 Mar 25;260(6):3440-50
references.pmid: 3838314
references.citation: Biochem Biophys Res Commun. 1997 Dec 18;241(2):312-6
references.pmid: 9425268
references.citation: Pharmacol Ther. 2014 Mar;141(3):272-82
references.pmid: 24201219
references.citation: Br J Pharmacol. 2008 Sep;155(1):4-16
references.pmid: 18552881
references.citation: J Biol Chem. 1990 Oct 25;265(30):18059-62
references.pmid: 2145278
references.citation: Acta Diabetol. 2015 Apr;52(2):315-22
references.pmid: 25182144
references.citation: Endocr Rev. 2003 Dec;24(6):719-36
references.pmid: 14671000
references.citation: PLoS One. 2014 Feb 21;9(2):e89292
references.pmid: 24586666
references.citation: J Biol Chem. 2004 Mar 26;279(13):12427-37
references.pmid: 14718545
references.citation: Front Biosci (Elite Ed). 2012 Jan 01;4:2253-68
references.pmid: 22202035
references.citation: J Biol Chem. 1993 Oct 5;268(28):20812-7
references.pmid: 8407910
references.citation: Nutr Hosp. 2012 Jul-Aug;27(4):978-90
references.pmid: 23165533
references.citation: Crit Rev Food Sci Nutr. 2016;56(5):788-801
references.pmid: 25118147
references.citation: Pflugers Arch. 1995 Oct;430(6):923-35
references.pmid: 8594545
references.citation: J Lipid Res. 2000 Feb;41(2):277-84
references.pmid: 10681412
references.citation: Am J Respir Cell Mol Biol. 2014 Jul;51(1):68-76
references.pmid: 24471656
references.citation: J Biol Chem. 1986 Nov 25;261(33):15424-9
references.pmid: 3096987
references.citation: Cell Calcium. 1986 Feb;7(1):1-12
references.pmid: 2420465
references.citation: Pflugers Arch. 2014 Apr;466(4):689-700
references.pmid: 24531745
references.citation: Biochem J. 1990 May 1;267(3):727-32
references.pmid: 2140257
references.citation: Bioessays. 1995 Jun;17(6):491-500
references.pmid: 7575490
references.citation: Biochem J. 1991 Dec 15;280 ( Pt 3):783-9
references.pmid: 1764041