title: Relationship of Fas, FasL, p53 and bcl-2 expression in human non-small cell lung carcinomas.
abstract: OBJECTIVE
  Lack of surface Fas expression is a main route for apoptotic resistance which is considered an important mechanism of tumorigenesis and tumor progression. Fas and FasL expression in 110 non-small cell lung carcinomas (NSCLCs) were investigated to evaluate their roles in pulmonary carcinogenesis and to examine the clinicopathologic significance of Fas expression with its relationship with p53 and bcl-2 over- expression.
  
  
  METHODS
  Immunohistochemical analysis using tissue microarray demonstrated that a large proportion of NSCLC patients (60%) showed lack of membranous Fas expression. The Fas-negative cases revealed the significantly lower survival rate than Fas-positive ones. Also, the loss of Fas receptor expression was found more frequently in advanced stage and higher nodal status. FasL protein was increased in most NSCLCs (89%) compared to normal lungs.
  
  
  RESULTS
  p53 and bcl-2 overexpression showed no association with Fas expression. Conclusively, reduced membranous Fas expression as a mechanism of apoptotic resistance is considered to play an important part of the pulmonary carcinogenesis, which may predict poor survival and have a negative prognostic influence.
  
  
  CONCLUSIONS
  Increased FasL expression is thought to be a basis for the immune evasion in NSCLCs. The rare bcl-2 overexpression suggests that this anti-apoptotic protein is unlikely to play a role in the apoptotic resistance of NSCLCs.
authors.affiliation: Department of Thoracic Surgery, Tumor Hospital of Henan Zhengzhou 450008, Henan Province, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Huai'an First People's Hospital, Nanjing Madical University Huaian, 223300, Jiangsu Province, China.
authors.forename: Ke-Ping
authors.initials: KP
authors.lastname: Xu
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Soochow University Suzhou 215006, Jiangsu Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Soochow University Suzhou 215006, Jiangsu Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Soochow University Suzhou 215006, Jiangsu Province, China.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Ge
authors.affiliation: Department of Cardio-Thoracic Surgery, First Affiliated Hospital of Soochow University Suzhou 215006, Jiangsu Province, China.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000276:immunology / Q000401:mortality / Q000473:pathology / Q000601:surgery; D053222:Fas Ligand Protein / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000737:chemistry* / Q000276:immunology / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D013997:Time Factors; D046888:Tissue Array Analysis; D019139:Tumor Escape; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D019014:fas Receptor / Q000032:analysis*
chemical_list: C000595707:BCL2 protein, human; D014408:Biomarkers, Tumor; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor
keywords: Antigens; CD95; FasL protein; carcinogenesis; carcinoma; non-small-cell lung
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