title: [Protective effect of magnesium isoglycyrrhizinate combinated with verapamil on liver ischemia-reperfusion injury after semi-hepatectomy in rats].
abstract: OBJECTIVE
  To investigate protective effect of isoglycyrrhizinate combinated with verapamil on liver ischemia-reperfusion injury after semi-hepatectomy in rats.
  
  
  METHODS
  Male SD rats were randomly divided into five groups: false operation (group A); rats subjected to 50％hepatectomy and 45 min of hepatic ischemia (group B); rats subjected to 50% hepatectomy and 45 min of hepatic ischemia, and treated with isoglycyrrhizinate (group C); rats subjected to 50% hepatectomy and 45 min of hepatic ischemia, and treated with verapamil (group D); rats subjected to 50% hepatectomy and 45 min of hepatic ischemia, and treated with isoglycyrrhizinate and verapamil (group E). Liver function, as well as morphology, SOD activity, FasL, caspase 3, and β-catenin expression of rats were analyzed.
  
  
  RESULTS
  Compared to group B, the levels of serum ALT of group E were significantly lower (P<0.05), SOD activity of liver tissue (98.8±7.1) were significantly higher (P<0.01), pathological changes were milder, FasL gene (1.327±0.193) and protein (0.010 9±0.001 4) expression were significantly lower (P<0.01). Caspase 3 protein expression levels (0.141 0±0.005 3) were significantly lower (P<0.001), β-catenin protein expression levels (0.079 1±0.008 2) were higher significantly (P<0.01).
  
  
  CONCLUSIONS
  Isoglycyrrhizinate combinated with verapamil can effectively reduce rats liver ischemia-reperfusion injury. The protective effect on liver cells may be mediated by inhibiting apoptosis caused by Fas/FasL system, increasing β-catenin protein expression, promoting liver regeneration and relieving liver peroxidation damage.
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China; Email: chenzubing@aliyun.com.
authors.forename: Zubing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Guan
authors.initials: G
authors.lastname: Tan
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
mesh_terms: D000818:Animals; D053148:Caspase 3; D006498:Hepatectomy; D022781:Hepatocytes; D007511:Ischemia; D008099:Liver; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D012503:Saponins; D014315:Triterpenes; D014700:Verapamil; D051176:beta Catenin
chemical_list: C521270:18alpha,20beta-hydroxy-11-oxo-norolean-12-en-3beta-yl-2-O-beta-D-glucopyranurosyl-alpha-D-glucopyranosiduronate magnesium tetrahydrate; D012503:Saponins; D014315:Triterpenes; D051176:beta Catenin; D014700:Verapamil; C505410:Casp3 protein, rat; D053148:Caspase 3